LOCUS       DAOSWR010000001        47049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_474_length_47049_cov_5.413372, whole genome shotgun sequence.
ACCESSION   DAOSWR010000001 DAOSWR010000000
VERSION     DAOSWR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 47049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47049
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_47049_cov_5.413372"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK513_00005"
     CDS             <1..721
                     /locus_tag="PK513_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOO80872.1"
                     /translation="TRAMKAQNQNQFIVCGEAPDVDKAKQIDKSYLQKGDLEIHKIIQ
                     GGKGLEVRFTWKNVTYNVEAPLFGIHHGHNLAMAFVAAFELGVSSKDIQAALPSLPQI
                     EHRLEVRKQSGGLTIIDDAYNSNPLGFQAALGLLSQIGTGRKILITPGMVELGKIHDE
                     AHKSIGAYAGQVCDVAIVVKGKRIPTFISAFKETGASKQLHEVESFDEAQKWIAKNKQ
                     DGDVVLIENDLPDIYERVPKI"
     gene            complement(797..1807)
                     /gene="murB"
                     /locus_tag="PK513_00010"
     CDS             complement(797..1807)
                     /gene="murB"
                     /locus_tag="PK513_00010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOO80873.1"
                     /translation="MKGIRGTLTENAPLGAQSWFRCGGHADLLFQPADADDLAVFLKV
                     WPLTPKKSPPGEGRGSTKTPLPLSGGGLGWGGKSAWGGNLTIIGGMANTIVRDGGIRG
                     ATIQLGKPFAEVKVLDGRYIQAGCGALNGNVAAAAVKAGIGGLEFLSGIPGTLGGALR
                     MNAGAYGSEIKDVLIGVNAITRAGHEIRIVLEDLKMSYRHTELPEEMIFTSAILKGVA
                     EDYETVKARITEIKKKRNDTQPIKEKTGGSTFANPPPAELRKAGLPEDTRAWQVVEKV
                     GARGLRVGGAQMSKKHCNFMINTGDATARDLEMLGEEIRKRAKHDLGLTLHWEIQRVG
                     EV"
     gene            2076..2252
                     /locus_tag="PK513_00015"
     CDS             2076..2252
                     /locus_tag="PK513_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80874.1"
                     /translation="MNTDQHGYYSIHCEGAKTRSGSFDFVQYVSFLVHRWNIARINPD
                     YQIPAQGGDDGDDN"
     gene            complement(2313..3755)
                     /gene="murC"
                     /locus_tag="PK513_00020"
     CDS             complement(2313..3755)
                     /gene="murC"
                     /locus_tag="PK513_00020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOO80875.1"
                     /translation="MQHGLPKDIGALHFIGIGGIGMSGIAEILNSLDYTVRGSDMAEG
                     PNVKRLRETGIEVKVGHAPENLKAADGNLPAAVVISSAIKKTNPELQAARDLGLPIVR
                     RAEMLAELMRLKWAVAIGGTHGKTTTTSMVGQMLETAGFDPTIINGGIVNAYGTNTRL
                     GASDIMVVEADESDGTFTRLPASVCVVTNMDPEHMEHYGTFEAVKDAYRQFIHNVPFY
                     GYAVLCIDHPEVQALIPSVKDRKIITYGFNPQADVQVSNVKSGPDGSVFDVSLPDERM
                     LRDVHLPMLGQHNILNALAALCIAYEMNVAAPIMKKALEGFTGVKRRFTKTGVSHDIT
                     VIDDYAHHPIEIETVLKAARMAVAENNARVIAVMQPHRYTRLHNLFDEFCKCFNDADA
                     VIIADIYAAGEDPIEGTDKRSLVEGIKNHGHREVMALPDRKELAHMIARLARPGDYVI
                     CMGAGDITGWAYDLPAQLDTELDKAKGNAA"
     gene            complement(3905..4585)
                     /locus_tag="PK513_00025"
     CDS             complement(3905..4585)
                     /locus_tag="PK513_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80876.1"
                     /translation="MSLKNRVMALSMLFGATQLSGCATSLYGDKGQELTIAFGNPEGA
                     EKIIAIDHRVVRIPSYDNDAFSIYGGKVKQDTDVGISTEASIAIEGEQAQAQGVGRSP
                     EVEADNLYHAGAADADTNRILIANTYVNIDLAPDYGELACKETLLKFNEITNTLSGVA
                     HLVDDADCADMKYYKNPQGYNFEFGGITGCFASKKVSPERLKASPNGQVVVALDSVPV
                     ALCYEYVR"
     gene            complement(4663..5766)
                     /gene="murG"
                     /locus_tag="PK513_00030"
     CDS             complement(4663..5766)
                     /gene="murG"
                     /locus_tag="PK513_00030"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOO80877.1"
                     /translation="MSDPLIILSAGGTGGHIMPAQALALDLISRGYRVEMVTDSRGMK
                     YAELFKDIPLHEIKAGTLGAGIKGKIKGAFDLGLGILQARALLKKTRPAIVVGFGGYP
                     SFPAVYAAQGMKIPTILHEQNAVIGKANEMLAPKAARIASSTPSLRGLEEAEKIHTIF
                     TGNPVRPEIAALYTKPYPKLDHDGELRILVMGGSLGAHVFSEVLPKALAGLPADHRAR
                     LHIVQQCRQEDLQETKKRYDEAKINAHLSPFIDNVAEELEHCHLFIGRSGASTVAEMS
                     AAGRPAIFVPYPHHKDQQQKMNAEIVSDAGGGWVMAESGFTSEALLARIETFLQNPQI
                     LFSAAEKARECGRPDAARKLGNLVTAIMSGWNE"
     gene            complement(5763..6932)
                     /locus_tag="PK513_00035"
     CDS             complement(5763..6932)
                     /locus_tag="PK513_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOO80878.1"
                     /translation="MSLFARTDQSPLGQWWWTVDRAMLAALITLAVFGIALVATASPP
                     VAERIGLENYHFLKRHLVILVPAIGMLIGFSFLGQRNIRRLAALAFVGSAIAMIAVLV
                     TGMEIKGAQRWIHIFGFSLQPSEFIKPAFAVLAAWFMAMQKEKEKFPGNAITAGLYFL
                     TITLLLLQPDLGMTVIVTAIWAAQIFLAGFPFRLLAVLLVLGVGGLVTAYAAFDHVQS
                     RIDRYLHPEAGDTYQIDKSLQAFQNGGLFGTGPGQGTVKMGLPDAHADFIFSVAAEEM
                     GLVFVLIILGVYGFILLRGYNRLMDSHDMFVVLAVGGLLTMFGLQALVHMGSSLHLLP
                     TKGMTLPLISYGGSSLLSVGFSMGIVLALTRRSRRTGISKGGLSLATSKTQGAAP"
     gene            complement(6929..8362)
                     /gene="murD"
                     /locus_tag="PK513_00040"
     CDS             complement(6929..8362)
                     /gene="murD"
                     /locus_tag="PK513_00040"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOO80879.1"
                     /translation="MIDCTKFIETLDGKPVAVFGLGLSGISTIKALVKAGAKVKAWDD
                     LADTHDAASETGAEITHLDQKNLQECALLVLAPGVPLFYPRPHQVVMTARKLGMEIIS
                     DLEILHRCNHGCKTIGITGTNGKSTTTALIGYILESAGCSVAMGGNIGRPVLDLDLPK
                     KDGFFVLEISSYQMDLCTTFTPDISVLLNITPDHLDRHGNFKNYAAAKERIFKGEGLA
                     IISLDDEPCRRIYERMMAYETGQRRVRPLSFEGAVENGVFVKDGILFDARKGASREIG
                     SIKGFASLRGIHNHQNAAAAYAVCASQDVALEPDVILEAMKTFPGLPHRQFQTRVING
                     IPYINDSKATNVEATSKALSAYRNIYWIAGGRPKGDNLEDISSLMSNVRHAFLIGEAM
                     IAYAGWLDQYSVPYTLCGDLENAVNEAHNLAQSERGRPGGGGVVMLSPACASYDQFRS
                     FEERGEYFTNYVNALDETQTSVDGAAA"
     gene            complement(8366..9451)
                     /gene="mraY"
                     /locus_tag="PK513_00045"
     CDS             complement(8366..9451)
                     /gene="mraY"
                     /locus_tag="PK513_00045"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOO80880.1"
                     /translation="MLYHLLFPLSEQFILFNLFQYLTFRTGGAILTALLICFLIAPRM
                     IGWLKAKQKEGQPIREDGPETHFAKAGTPTMGGLMILISVTLSTLLWADLSNQFVWIA
                     LLVMVGYGLIGFGDDYLKLTKRNTKGLPGKLKLAAQILIGSIAAIWIMNALPGHINQH
                     IAIPVLKDIYIYIGLLFIPWALLVMVGSSNAVNLTDGLDGLAIVPVAIAAGVFGMISY
                     LVGNEVFSEYLRIPYVVGTGELSILCGALIGGAMGFLWYNAPPAMIFMGDTGSLAMGG
                     VLGTIAVIVKHELVLAIVGGLFVLETVSVIVQVVSFKLTGKRVFAMAPLHHHFEKKGW
                     SEPTIVIRFWIIAFILALVGLATLKLR"
     gene            complement(9491..9931)
                     /locus_tag="PK513_00050"
     CDS             complement(9491..9931)
                     /locus_tag="PK513_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80881.1"
                     /translation="MLGSNSLNWKAVRLAGVTVSVQPRPRTPYSVLKVGGLVDTGNAQ
                     QTVILQNALPSEWEIDLENKTKSSKGLSLPRRIANLNCIYTGRSFGVVADVTDAVRAV
                     TRNAKLAAIVPEVVAPGDFMIFKHVGPRFRIRFAGMLRAVPQMR"
     gene            complement(9931..11382)
                     /locus_tag="PK513_00055"
     CDS             complement(9931..11382)
                     /locus_tag="PK513_00055"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOO80882.1"
                     /translation="MNFDVSTIKGLSEDSRAVKKGYLFAALPGCKVDGRDYINAAIEH
                     GASVILAPEGTQLPPGVKDVKLLTDPNPRQALAKLAAQFYARQPEHIAVVSGTNGKTS
                     TTLFVQQLWTAAGYNAASLGTLGVRCAGVVKSGAMTTPGPVALQAGLAELAAEGVTHL
                     AMEASSHGLDQYRLDGVEFEAAGFTNLSHDHLDYHGSMEEYFTAKARLFEELLPESGV
                     AVLNADIPEFEKLQEICKRRGVKILSYGHHGKDLKILSVKPAGHGQDLELGITGAHKD
                     ITLPLVGEFQVMNALCALGLVIANDPAKKEIYIEALEKLQGAPGRLQLVPGHPGAAVY
                     VDYAHTPDALENILKALRPHTKGKLVCVFGCGGNRDKAKRPVMGAVAGQYADLTIVTD
                     DNPRGENPADIRAAILSSVPSSQQIADRREAITTAITQLETGDVLVIAGKGHEQGQIF
                     ASHTTHFDDVEEAGKAIAYLEHQEPSKEEEKKV"
     gene            complement(11382..13109)
                     /locus_tag="PK513_00060"
     CDS             complement(11382..13109)
                     /locus_tag="PK513_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOO80883.1"
                     /translation="MIFSQRLRRNTVKIEGERSSALDLARGRLVLMSGFFILVYMVFA
                     VRAFDLAIIQGHAIAPDNGQIAESNMLNINGETPRRADITDRNGALLATTLKTASLYA
                     DPRLIADADAAAKKLIIIFPDLKYGDILQKLQSQKRFVWLQRNVSPDQQYQVLQIGEP
                     GLEFEEGYKRFYPQGALAAHLLGYGNVDAQGLAGIERSFNKHLGGGHDLKLTLDIRLQ
                     HVLRREMLKAVEEFEAIGAAGAIMDVTTGEILAGVSLPDFNPHDPGRADKDAIFNRLT
                     LGAYELGSVFKIFSTAAFFEAHDVPMSTTFDASEPIKIGRHTIHDYHAEDRVLTIPEV
                     FMYSSNIGSALMGRAVGTDALRSFYRDLGLLDPLDFEVREVTRPLVPHPWREVNTLTA
                     SYGHGVSTTPLQLITAVSSIVNGGYLVKPSLVLDEQSDEEEPGKDIRIVSAETAQRMR
                     QLLRLVVTDGTAKKAEVKGYRVGGKTGTAEKIADGRYDSKKKISSFVGIFPMDEPRYA
                     VFIMVDEPKGQKHTWNYATGGWVAAPAVARVIASMAAILGISPAGDNTPENHFGESLK
                     QFVSTKEEH"
     gene            complement(13106..13612)
                     /locus_tag="PK513_00065"
     CDS             complement(13106..13612)
                     /locus_tag="PK513_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80884.1"
                     /translation="MKLLKPRTFIIFALVGLAGAVLLHTSQNVQHAQERLEALEQSVQ
                     REEEKIRMLKAEWETLNRPERLERLANEFLDLVPPSPDQMTSEEVHLPESVPSEFVLP
                     EVESFPPPVLQPVTFQSAGQEKESAPIPVQKPQRADPSMRHNKPVKEKAFGDLLNELG
                     QSKNGGVP"
     gene            complement(13609..14553)
                     /gene="rsmH"
                     /locus_tag="PK513_00070"
     CDS             complement(13609..14553)
                     /gene="rsmH"
                     /locus_tag="PK513_00070"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOO80885.1"
                     /translation="MKGLKRLSHYPVMLNEVLAALSPVDGGVYVDGTFGAGGYSRAIL
                     DAADCTVVAIDRDPDAAKRAEDLSRNYAGRLIFLRGCFGDVESLLAGAGIEKVDGFVL
                     DLGVSSFQLDQAERGFSFRADAPLDMRMDPESGKGAADLIAQMDEKELADLIYKYGEE
                     RKSRQIARRIVEMRRAAPIETTGQLAEIVRCCVPKSRDGIDPATRTFQALRIAVNDEL
                     GELERALNAAENILKPGGRMVVISFHSLEDGLVKRFFRSQSGAEAVSRYLPQTENDPA
                     KLRLLTRKAVKPGEQEIAENSRSRSARLRGAQYIGGAS"
     gene            14540..14824
                     /locus_tag="PK513_00075"
     CDS             14540..14824
                     /locus_tag="PK513_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80886.1"
                     /translation="MSPFIESKQCVTQRCENRFWIRKEAYEQALTLLEVSEEEEIRQF
                     IRRVAPRVVPIYLFVEESFLYFILSFSMKKSAQTPRNLSRNGSRGPQSWA"
     gene            14873..15237
                     /gene="rnpB"
                     /locus_tag="PK513_00080"
     ncRNA           14873..15237
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK513_00080"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(15385..15828)
                     /locus_tag="PK513_00085"
                     /pseudo
     CDS             complement(15385..15828)
                     /locus_tag="PK513_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            15911..16570
                     /gene="msrA"
                     /locus_tag="PK513_00090"
     CDS             15911..16570
                     /gene="msrA"
                     /locus_tag="PK513_00090"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOO80887.1"
                     /translation="MNNFFVPVLMFAGLVVLLWFAASPQRSMAEKGLGRAHLGGDLME
                     MKPEGYPVAIFAGGCFWCTESEYRSQPGVLYTRVGYIGGHMDNPKYEDTHGSKSGHAE
                     AVEVTYDPDKTDFRALLMFFFTQAHDPTQLNRQGPDVGTQYRSAVFYTDEAQKRQAQE
                     VIDELTAAKRFAKPIVTTLEPAGTFWEAEDYHQRYYEKYEAKTGRPHINDWLARQRRG
                     D"
     gene            complement(16773..17465)
                     /locus_tag="PK513_00095"
     CDS             complement(16773..17465)
                     /locus_tag="PK513_00095"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOO80888.1"
                     /translation="MNTRMKMDIEALKKTYVKPDKVQAQKILRYVKDFENEKDRFAFI
                     GMGGTISSGYNPNKESLFPFPCHVSRTLIEDLQGKFGINTDTFASAELSSKDSRDLEN
                     ADLEALLDFVSSIENERILITCGTYLLTKIAEILIDTDVVQDKIVAVTGSMLPTGFTF
                     SDSGMNIMSAITAVDLAKKHELKPSHNVFVVFHGQVYTTLEEIKALNLHNEATDKLVI
                     EYPLTSVPYKRG"
     gene            complement(17524..20385)
                     /gene="ileS"
                     /locus_tag="PK513_00100"
     CDS             complement(17524..20385)
                     /gene="ileS"
                     /locus_tag="PK513_00100"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOO80889.1"
                     /translation="MNKDSDNKDKYKDTVFLPKTDFPMRGGLPQKEPEILQQWQDMDL
                     YGQIRENTKGKERWVLHDGPPYANGDIHMGHALNKILKDVVVKSYSMLGYDAPYVPGW
                     DCHGLPIEWKIEEQYRNAGKDKDEVAPLAFRDECRKFAQGWVETQSAQFQRLGVSGDW
                     KDPYLTMTKRAEGKIVREIHEFVRNGGLYKGAKPVMWSVVEKTALAEAEVEYKEHKSI
                     TIWIKFPIIDCPNEPLLEGANVVIWTTTPWTMPSNRAIAFGEIHYALYEVEEVAEDSR
                     AIVGERLLLAENLAEDVKEKAKIIRWSKLRAWGNLNPVGDGEGSGLFYNTTCQHPLHG
                     QGYDFDVPLLFGNFVTEDAGTGFVHIAPGHGEDDFYLGTANGIEVTDNVADDGKFRPH
                     VPLFAGLEVYTQDGKLGDGNFAVLKAMDEAGKLLAKGSLKHEYPHSWRSKAPLIFRTT
                     PQWFISMDKNDLRGKALTAIGDTKWVPAKGEARIRAMIAGRPDWCISRQRAWGVPIAL
                     FVHKETDEILRDETVLNRIGDIFEAEGSDAWWSRNPQDFLGNDYNAADYTQIFDIVDV
                     WFESGSTHAFVCEDRDDLRRPAGKRPANLYLEGSDQHRGWFHSSLLESCGTRGTAPYD
                     TVLTHGFVLDEKGYKMSKSLGNVVDPLKLMEQSGADILRLWALTCDFSEDIRIGKDSI
                     KTTGDLYRRIRNTLRFLLGALDGFTEAERVDLSDERVPELERLVLHQLTEMDTKLRTC
                     IEKYDFMRLTKALHDFCNEELSAFYFDIRKDRLYCDEPASFERRATRTVMAEIFGCLV
                     SWLAPVLVFTAEEAWSHRPAGVFGDEESIHLREFPTISESWKNPELAEKWAEIMTIRK
                     SVLEAIEPLRADKTLGSSLEALPTISTQSHYVKSIAGEMADICIVSDLQVKEGDPSVK
                     IEKAPGQKCSRCWKVLPEVSQNGDLCNRCTDVVKEAKAA"
     gene            complement(20581..20805)
                     /locus_tag="PK513_00105"
     CDS             complement(20581..20805)
                     /locus_tag="PK513_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80890.1"
                     /translation="MMTAQAGAVPLRQAAFPQAAQGMEGETVTRPMMDGMPLSSGQTY
                     PGQYASTASPNMQKKANPFAMTFDGYPLNF"
     assembly_gap    20880..20979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20980..21191
                     /locus_tag="PK513_00110"
     CDS             <20980..21191
                     /locus_tag="PK513_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80891.1"
                     /translation="LAVKNSEDYESKGRTGQAFSGSFLTTPHFFKNVSFPSLRGAIGD
                     KAIQEIRVFWIAALPLVARDDEIEG"
     gene            complement(21243..22163)
                     /locus_tag="PK513_00115"
     CDS             complement(21243..22163)
                     /locus_tag="PK513_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOO80892.1"
                     /translation="MDIFTTLFTNLIPLYMLIGLGWIAGRYFDVDRQSLGSLGIYILM
                     PVVAFGFVAKIELQPVYILLPIIVFALFSWLTLLYYMLAQRIYDDHRANLLAMCGGAG
                     NTGYFGLPIVLLLFDPEWVGVYIFALVGGLVYEATVMYYIANRGRFNMRDSLIKLFKF
                     PSIYAVIAGLGVNMAGTELPALFNNYWEHFKGAYVLVGMMIIGASLAKAEKLIIAPKF
                     LALTFVGKFIIWPAAAYGLILFDQGVTGWFGPEIHKMVFLMAIVPPAANIAAFAAKLD
                     MSPEKAATTILIGTIFALFYIPLILLVSGL"
     gene            complement(22255..23190)
                     /locus_tag="PK513_00120"
     CDS             complement(22255..23190)
                     /locus_tag="PK513_00120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOO80893.1"
                     /translation="MEVFERLENITDSAKGAVIAIGNFDGVHRGHQALLGRAKDIAVS
                     EGKSFGVLTFEPHPRRLFRPDDPPSRITPPEVKHWRLAEFGVDFVVSLPFDWDFASQS
                     AADFITGVLLGGLHPHHIVIGEDFRFGQLRKGEPEDIRDAGIALTTVEKIVGDDGKVF
                     SSSAVRSALRIGDLGAANENLGWNWEIRGRVVKGDQRGRQLGYPTANVALHETVHPAY
                     GVYATHVQIEGEEEWHNAATNIGIRPMFEVPTAQVESFIFDFDREIYGKTLRIRPVQR
                     LRSEAKFTSLDALIDQMEKDCAQAREILSIHTPPP"
     gene            23350..23697
                     /locus_tag="PK513_00125"
     CDS             23350..23697
                     /locus_tag="PK513_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HOO80894.1"
                     /translation="MVSKKAVNTLLASAVALTVAGGIGAANAMDEGTEKCFGVVKAGH
                     NDCGNADKTHSCAGQAAEDGAGNEWIALPTGICEKLIGGSLEPTSGSADDVMHDEDMH
                     EDNMHDEHEGEGH"
     gene            23701..24606
                     /locus_tag="PK513_00130"
     CDS             23701..24606
                     /locus_tag="PK513_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOO80895.1"
                     /translation="MTSHSTITNRADQLFPPAKIPYEPVGIGFRTPHYEEVLETDLNL
                     GWLEVHPENYFGGGVHRHFLSEVRRKYQISLHAVGLSLGSDQPVSEDHLARVRELIDI
                     YDPFSVSDHASWSASGNAHLNDLLPLPYTQASLNKLARNVERAQEALGRRMLIENPST
                     YLAFKGNEMHEDTFMNKLADMTGCGILLDLNNIYVQAHNHDYDAWSYVDMIEAHHVGE
                     MHLAGHIEQTAGEGTLLVDTHSRPVKGDVWGLYEHAIKRIGVVPTLIEWDNDIPDLAT
                     LVSEAAKARAIIQKVRAEDLPDAAE"
     gene            24723..25499
                     /locus_tag="PK513_00135"
     CDS             24723..25499
                     /locus_tag="PK513_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOO80896.1"
                     /translation="MRLNEFQKRFKDLMLDHPDALHAPPEDLVDFCRSGDIALPERLK
                     VYRNNIVGSLTDVMLATFPVMEKLVGKEFLEMMARSFILKNPPNHGCLSLYGEGFAEF
                     IEGFELAKSLPYLPDVARFELALNSAYYAADDRPLTAEALGRIAPEHLENLKLVLRDS
                     VQLLRSRYPLSAIQDFCMSGAQEGTLNLDQGGEHLMVFRPGLNTQTVLLDEDEYLMLV
                     LLADGQTLGAAVEKVLETHPDFDFQNFLQKHMSLETFRAL"
     gene            25618..26169
                     /gene="lspA"
                     /locus_tag="PK513_00140"
     CDS             25618..26169
                     /gene="lspA"
                     /locus_tag="PK513_00140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO80897.1"
                     /translation="MQKFPYLILALVIFTTDQISKWFVTEVMIKVVVEKPMPSTLGFF
                     EWLSSAPARLPPVSIEILPFFNIVMVWNQGVSFGMFSNGLHWGPWFLSGLSVIISIGF
                     LIWLSRSFSRLQSVAIVMVIAGALGNVVDRMRFGAVIDFLDVHALGYHWPAFNIADSA
                     ICIGVFLLIIQSFFFETGGKNAK"
     gene            26198..26716
                     /locus_tag="PK513_00145"
     CDS             26198..26716
                     /locus_tag="PK513_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HOO80898.1"
                     /translation="MKSFNTIFLVLVGLVALSACETAQEQLGLTKEAPDEFKVVKRAP
                     LEMPPNYTLRPPSPGAARPQEQETAEEARKTVLGNASAQNTYTPSGSEAALLQAAGAT
                     QTDPAIRRKVDEETANMRDENEPVIEKIMSIGRDRPPPAKVVDAAKEAQRLQKNQAEG
                     KPVTSGETPSVE"
     gene            26734..28185
                     /locus_tag="PK513_00150"
     CDS             26734..28185
                     /locus_tag="PK513_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO80899.1"
                     /translation="MFYSLRLCKSLLVLSVLVASILPFTALAEKQELPDERAATPDAP
                     QEERRRVFNAESFTLDNGMEVVVIPNDRAPVVTHMVWYKFGAADEPIGKSGIAHFLEH
                     LMFKGSAQVGGRDLDPGEFSEIIRSMGGNDNAFTSQDYISYFQSVPAQHLETVMRMEA
                     GRINDINPPADEVLSERKVILEERKQRTDNDPRGQFGEQLAAAAFVNHPYGIPVIGWK
                     HEMDELSYEDAKAFYDRWYAPNNAILVVSGDVTPGRVFQLAIDIYGQLPREDVPKRER
                     TRSPALNSHTSVTLEHPAIREPLVQTLFRVPSARQNKEESLALQVLEEILGGGPTSRL
                     YKSLVVDQKIASSAGISYNSAAWDDSSLWIYATPLQGKTLSDIQTALMDELRKLIKDG
                     MREKELGDAKSRMSDEAVYARDSLTGPAMVIGYGLATGQSLDDIEYWPYDISEVTADQ
                     IKAVAEKYLNPDAPGEHPPVSGYLLPQAEGDKK"
     gene            28182..29612
                     /locus_tag="PK513_00155"
     CDS             28182..29612
                     /locus_tag="PK513_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO80900.1"
                     /translation="MITRLSLQGGQSPTRQSKLVFYPKGHKWIASLALAMTLFWVMPA
                     QAEDGLLDIQEVTSESGISAWLVEDHSIPVIAVKFGWRGAGSARDPEDKQGLARMLSN
                     TMDEGAGSLNSQAFQKELRDLVISLSFSASRDDFTGSLKTLTKNKDRAFELLEMALTK
                     PRFDKEAVERMRQANQSRIRSSLSDPGWMAARLLNDKAFEGHPYALNSGGTLSSLERI
                     ESADLKKLHKSVLGRNNLSVAVAGDITAEELAGVLDQVFSDLPDILLDDLANLEIQNA
                     GKTFLYKRDIPQSVVEILQPGIGRDDPRFHSAQVMNFVLGSSGFGSRLTEEIREKRGL
                     TYGIYSYFYDLQHLKGLAVSTSTANENVPEMLKLIKDEFNKMVSSPIGEKELRDAKSY
                     LIGSLPLSLTSTDKIAGLMNSLQLDGRPVDYLDQREKAIEETSIEDIQTLATELLDPE
                     QFITIIVGEPEKLKDVIEVKSLPNVE"
     gene            29602..31242
                     /gene="pgi"
                     /locus_tag="PK513_00160"
     CDS             29602..31242
                     /gene="pgi"
                     /locus_tag="PK513_00160"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOO80901.1"
                     /translation="MSNSPAHMPVHERPEWEVLQAHQREISNMHMRDLFANDPQRFEN
                     FSAKIPGLLLDYSKHRITDETMNALLALARACNIERQRDAMFSGNKINSSENRAVLHT
                     ALRRSADEKIILDGEDIMSFVYKTLFRMRDFSDAVRSGTWTGCTGRRIKTVINIGVGG
                     SDLGPQMVCEALKPYAERMAESCGQTVDLAVRFVSNIDATMLHDTLQDCDPETTLFIV
                     ASKSFTTQETITNALGVKAWLLDTLSDETAIKRHFVALSANEEAAMQFGIPPENIFPM
                     KDWVGGRYSLWGAIGLSICCAAGFDVFQDLLAGASAMDHHFQSTPLDKNIPVIMAILG
                     IWNRNFQGYPAQAILPYAHHLQRFSTYIQQLDMESNGKGVTADGRPVSVASGPIIFGE
                     VGTNAQHTFMQLLHQSPEVIPADFIMVAKPVPPYENQHKKLLANALAQSKSLMEGQEN
                     SEEPHRNFPGNRPSSCVVLDTLDAYHLGLLLALYEHKIFVQGVIWGINSFDQWGVELG
                     KTNAKHIIDAFETHKKIDTLDSSTAGILRHLTQKFIKF"
     gene            31331..32632
                     /locus_tag="PK513_00165"
     CDS             31331..32632
                     /locus_tag="PK513_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOO80902.1"
                     /translation="MRASIPDPNELTRLDQSKLDEALRKHDMYLRGQIGGARCVLKYH
                     NLSELDFKQYDLSQADFTGSLLVEADLSYGVFISTCFFACDMRNANMHNADFSRADFR
                     GAYVAGANLSGANLSDVDLREGKIMKRDDKGVLEDRKRSGGTGSRAILSGAKMSDTNM
                     SRARAMSADFSDSDMSGVTMTDANLSGASFEGANLADADLSGSDLSSTNMRQSIMTGT
                     ILERTEQLDMDDTAAVTERDMGNKLENLGKSLPELLEEHTLWVATAGRAGRQLDLSGY
                     DLRDVLDLKRFPLTAIQCTGANFLNQDLRSAELQSGVFDRSDFRDCDMTEADLRGSSF
                     KYAQMARVNLTGALMCPLEFKKDMGHSTLRRVDFSGANFRFSNLRGADLRDAILMGVD
                     LTNAVLNDCDLRRADFTGAILSGVKMENCRLDEAIIDFKAL"
     gene            32708..34527
                     /gene="mutL"
                     /locus_tag="PK513_00170"
                     /pseudo
     CDS             32708..34527
                     /gene="mutL"
                     /locus_tag="PK513_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
     gene            34529..35074
                     /locus_tag="PK513_00175"
     CDS             34529..35074
                     /locus_tag="PK513_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80903.1"
                     /translation="MNEQPQSHGRKSVLNLYAALGASLILSVLPSVTAAGLSLVFFLG
                     VLIAAYHMRGKVEEHSLIENHATFIIRTLWIGAFLTLPTMGLATAYMMSGIDYAPFEV
                     CAQALANKGVAWMESAGMMEVYGLVEPCVEGFITENKVLFINAVVIAGAPVVIYMTYR
                     LIKGLSRASKGYRLAKPKGWF"
     gene            complement(35083..35364)
                     /locus_tag="PK513_00180"
     CDS             complement(35083..35364)
                     /locus_tag="PK513_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80904.1"
                     /translation="MSEDTGYEKGTDLVIKGEVGVNAQSNLQELFRENCQLTQLGMIF
                     ASDGSYVIEKDCGSAEVPEGVTASELEQANEVLKRVNEELQTPDPHRAF"
     gene            complement(35455..36165)
                     /locus_tag="PK513_00185"
     CDS             complement(35455..36165)
                     /locus_tag="PK513_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HOO80905.1"
                     /translation="MKKFYKLVSVHQEVSGWAVHLDSRPVKTPLKKTLLASSETMATA
                     IQEEWAAQGETIDPETMPLTQILSTQIDRVSEQRAAMSAELLKFLDTDLICYRAPKDQ
                     PAGQAEKQAESWDRWLTWFEDKFDCALQTTTDLAALKQPAAAHTAVQTKTDALDDARF
                     TALQLVVPLSGSLVLGLAFIEGAITPQEIYNAARVEEHFKDEIYNAEKYGRDPLSEKK
                     DAAMLRDLEAAAAFLNLL"
     gene            complement(36162..38027)
                     /locus_tag="PK513_00190"
     CDS             complement(36162..38027)
                     /locus_tag="PK513_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOO80906.1"
                     /translation="MSEENAPQVPEYKGWESVVLRILKWLAIIGVILFILATALSRLG
                     GNSPVLRESIEEFLTDTTPYTARIGTLNSMRFFPNIAIDITDVQLRGGDDGMGETMIR
                     IGAARLVMPFFDALFRPGHFKDIHIENLMAKPGSILNKSIEVSAMNVREQDARATFIV
                     EGAIDNSPLHAKFGLQTKGKPGRHTYVIGRQRDFEISLADLTARGSIHNTVIGGYKFE
                     NIVLLNKQKEVLHGHIDMDRASSGKLEFNGQLRLEPGKTRIDPDVTLDWTGETPLIFG
                     KVTSNELILSDVTGESPAQRTIDALDKIFGEQNAALDLSGLTLDLNLDVQKFKVGAVE
                     LGKLKAPLILEEGDLHIGPLEGKIVGGELSGHVDLLTSKSPAKLIQKIAVKGFDYRAL
                     QKQLSTGEVTIEGHGDILIDLSSQSTSMDGLIEGLSGKVGFVGGQGKMRAGLLDLWGS
                     GLLNALLPSFEKESDLNMNCVIVNLDVKNLKGHSDSVFVDTKSITLYGEGTYDFKADQ
                     LEMVLEPKPKNVAIGDIASAINVSGPLPDLKVSPNVFDLGKRVGGLLLGAVNPAFYAV
                     TLADFGLAQDHPCKAFIIEKEFLPAPEKPEVEAVPEAPDSKPQEAGEGQAKDLNK"
     gene            complement(38024..39043)
                     /locus_tag="PK513_00195"
     CDS             complement(38024..39043)
                     /locus_tag="PK513_00195"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO80907.1"
                     /translation="MSNVRQIEVKADDDGQRLDRWLKKNVPELPYGLAQKLIRKGQIR
                     ADGKRIKADAKLKAGQEIRIPPLEHKSIDEKVKITDKDKAFIKSMVIYEDADVIALNK
                     PAGLASQGGSKTKYHIDGLLEALTGKDGVKPRLVHRLDKDTSGILLLARNAKAAKAMG
                     DAFKTRKVKKIYWAVVCPAPDRPDGTIKAPLIKAGGNNKERMVVDEKEGKSAITDFIV
                     LESALHSAAFVAFWPRTGRTHQIRVHSELIGCPIVGDKKYARLPEQEETHEARRKAEA
                     DLKALNLADRLHLHARRIILPHPSGRKAPLDVTAPLPPELKKTWKALGFSPSLKADPF
                     EDTKS"
     gene            complement(39040..39420)
                     /gene="crcB"
                     /locus_tag="PK513_00200"
     CDS             complement(39040..39420)
                     /gene="crcB"
                     /locus_tag="PK513_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOO80908.1"
                     /translation="MNAILAIAAGGALGAVMRHGVNVGAVKLLGHGFPYGTLTVNIVG
                     SFIMGLLIAIFAHYWHPSETMRMFIVTGFLGAFTTFSTFSLDFVNLWERGSYLYSALY
                     LGGSVILSIAGLFAAMAIVRACVS"
     gene            39471..40361
                     /locus_tag="PK513_00205"
     CDS             39471..40361
                     /locus_tag="PK513_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO80909.1"
                     /translation="MFNHLHPSHKGIVLAFIGFTAFAFSDVVSKWLLQYYSTYQVVFI
                     NHSTACLYLLAALHMSASLKTLKRSTKISLHLFRGVLNTCVALSVIFSFSRLPIADVY
                     AFLFTVPFFSALLGVAFYKQAIGLHRGLAIVIGFTGVVIGLQPGSEGFSPWLIVPILA
                     GFLIALKFTISRSLEGENTLLLGLWPLLVSAPLAGLLSISSFEMLNFTHFAMHLFTGL
                     CIATGLVCVSQAFRIAPAAAVSPILYTEMIWALLFGFLIFGDAPDLMMMVGAGIIILS
                     GIYLVETERRNGRSNIKVKS"
     gene            40495..41226
                     /locus_tag="PK513_00210"
     CDS             40495..41226
                     /locus_tag="PK513_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005185835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOO80910.1"
                     /translation="MDWLFSVDLWMSFLTLVVLEIILGIDNIIFLSIVASRLPAEKQK
                     AARLIGLAFALLMRIALLLSISWLTHITVIVLSIAGHDVSWRDLVLGLGGLFLLYKGT
                     REIHSTVEGDDHDTANISKAATFGSVITQIVIIDMVFSLDSVITAVGMTDHVPVMIAA
                     VAIAIVMMMFAAEPASRFVQENLSIKMLALSFLLLVGVALIADAMHFHIPRGYLYFAI
                     AFSLGVESLTLLAQKRSEKRKAARA"
     gene            complement(41230..42519)
                     /locus_tag="PK513_00215"
     CDS             complement(41230..42519)
                     /locus_tag="PK513_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO80911.1"
                     /translation="MGDLFAVDQEGAALDERRPLADRLRPHCIEDVVGQDHIVGKGAP
                     LRKMLDGGHLSSLIFWGPPGCGKTTLARILAHHTDLHFEQLSAIFSGVGDLRKVFDAA
                     KIRKQNGRSTLLFVDEIHRFNKSQQDAFLPVMEDGTITLIGATTENPSFELNAALLSR
                     AQVFVLKRLDDEALEALLQKAEKDAPLPLAPEARALLKTMADGDGRYALSMAEQIIAQ
                     GEILDEEGLLALVQRRAPLYDKGNEAHYNLLSAFHKSLRASDVDGALYWMARMLVGGE
                     DPATILRRMTCMASEDISNADPQALVIANNAWTAYERLGLPEGELAMAQACTYLACAP
                     KSNASYMALKTAKRAAQETGSLMPPKHAMNAPTKLMKSEGYGLGYQYDHDTLEGFSGQ
                     SYFPDGLKRHEFYQPAERGFEREIKKRLEYWEKRRKS"
     gene            42610..43620
                     /gene="nadA"
                     /locus_tag="PK513_00220"
     CDS             42610..43620
                     /gene="nadA"
                     /locus_tag="PK513_00220"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOO80912.1"
                     /translation="MSAERLDYTGEVAKATTPLYERLKKQYSPERWQTLAPYIHEINR
                     LKKEKNAVILAHNYMTPDVFYGVGDVMGDSLMLAQKAAQSTADIIIQCGVHFMAETSK
                     ILCPDKKVIIPDMKAGCSLAESITGEDVRRLKEQHPGIPVVSYVNTSADVKAESDVCC
                     TSSNALKIVNALGEQGHDTVIMTPDKFLAQNVAAETDVKIIIWDGTCMVHERFTAEDV
                     KKMRADHPGIFVLAHPECPPEVMAEADYAGSTAQMDDYIKEHQPKMAVLYTECSMSDN
                     IAAANPGVEMIGTCQMCPHMKRITLPKILNALQDEGPEVEIDAEIAERAKAPIERMLE
                     LS"
     gene            43729..44574
                     /gene="nadC"
                     /locus_tag="PK513_00225"
     CDS             43729..44574
                     /gene="nadC"
                     /locus_tag="PK513_00225"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOO80913.1"
                     /translation="MTLSPIIIEDIVKTALKEDLGHGFDITSNLLIPAGQSARGVLRA
                     RQDGRLAGLIVALSTFSLTDIDFDMTVMTEDGADLSAGDVIAEIEGPVRSLLTAERVA
                     LNFMSHMSGVATLTARYVEKVKDTGVEICDTRKTLPGLRLLQKYAVEIGGGANHRFGL
                     DDAILIKDNHIAAVGSIDEALNQAHMLAGHTKKIEIEVDTLAQLEDVLANGKADIVML
                     DNFGLKDLKKAVQLCKKQITSEASGSVNLETVRTIAETGVDYISIGALTHSAPALDIA
                     LDIDV"
     gene            44567..44845
                     /locus_tag="PK513_00230"
     CDS             44567..44845
                     /locus_tag="PK513_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOO80914.1"
                     /translation="MCKTILQVKLTPNSTKNAVLGWEDGADGEKVLKCCVTAAPEKGK
                     ANNALISLLATHYGIPKRRLSLLHGEKSRIKIIEIQDIEGISNIAFKR"
     gene            44927..45511
                     /locus_tag="PK513_00235"
     CDS             44927..45511
                     /locus_tag="PK513_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80915.1"
                     /translation="MTLRVFKNHALITGLGAFMFFAHGSACRAAGNIETLTKQNVKEF
                     IEDTTDITTTNTQALSPEKIQQYLETHLDKKARFKSVMKYNIPGMPPQEAALSLDKEE
                     FMKSVVQGAEAVDGYETLVEITDIKISSDGEKAFVKTQNTEYATMPVATETGSVENVP
                     MEGVSECTQILLLKKGVIMMYNANCVTNIEFMEY"
     gene            complement(45515..46306)
                     /locus_tag="PK513_00240"
     CDS             complement(45515..46306)
                     /locus_tag="PK513_00240"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOO80916.1"
                     /translation="MAQFTLPANSKITAGQTHDVAGDAKRKKTFKIYRWNPDDEANPR
                     LDSFTIDLDKCGPMVLDALLYIKNNIDSGLTLRRSCREGICGSCAMNIDGTNTLACTK
                     AIDDCSGDIKVTPLPHMPVVKDLVPDLNHVYAQYTSIEPWLKADSPAPGRERLQSPED
                     ANILNGDDGRGPAGCILCFCCSTSCPSYWWNGDRFMGPAILLQAYRWIADTRDEATGD
                     RLDNLEDPFRLYRCHTIMNCTNTCPKGLNPAKAIAEIKKLMVMRR"
     gene            complement(46416..>47049)
                     /locus_tag="PK513_00245"
     CDS             complement(46416..>47049)
                     /locus_tag="PK513_00245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO80917.1"
                     /translation="QEDGNKAQGEKTLIQQLEQANQRASVDRNTSIRDSLTGLYNRRY
                     FDDALPKTLTKLRRSGGRIALIMVDIDHFKKFNDTYGHQVGDAVLRKAAEHLQESVRA
                     DDIVARYGGEEMAVIMEVNNGNDVDPAFTRLAGEMCAFTIEHKGEEYKIGSSIGIGIS
                     GPAITPENIIDAADKALYRVKQTTRGRMEIYDLSVFERRQTLDVKPDAPE"
BASE COUNT        11053 a        11420 c        12680 g        11796 t
ORIGIN      
        1 cacacgcgcg atgaaagcgc aaaaccagaa ccaattcatt gtttgcggcg aagccccgga
       61 tgtcgataaa gcaaaacaga tcgacaaatc ctatttgcaa aaaggcgatc tggagattca
      121 taaaattatt cagggcggta aagggcttga agtgcgtttt acgtggaaga acgtcactta
      181 caatgtcgaa gcgccgctgt tcggcatcca tcacggccac aatcttgcga tggcctttgt
      241 ggcggcgttc gagcttggcg tttcgtcgaa agatattcag gccgccttgc cgagtttacc
      301 gcaaatcgag caccgattag aggtgcgtaa acaatcgggc ggtttgacga ttatcgatga
      361 tgcgtataat tccaatccgc ttgggtttca ggccgcgctg ggattgttgt cgcaaatcgg
      421 cacagggcgc aaaattctga ttacgccggg catggttgag ctgggcaaaa ttcacgatga
      481 agcgcataaa agcatcggtg cctatgccgg acaagtttgt gatgtggcga ttgtcgttaa
      541 aggtaagcgg attccgacct ttatcagcgc gtttaaagaa acgggagcca gcaagcaact
      601 gcatgaggtt gagagctttg acgaggcgca aaaatggatt gcgaagaaca agcaggatgg
      661 cgatgtggtg ctgattgaaa acgatctgcc ggatatttac gagcgcgtgc cgaagattta
      721 aggctttcat cgtttgattt ggggtgtaat atttctggat tgccgcgctc ccgttggtcg
      781 ctcgcaatga cagcagtcac acctctccaa cccgttgaat ttcccaatgc agggtgaggc
      841 cgaggtcgtg ctttgcgcgt ttgcggattt cctcgccgag catttcgagg tcgcgggcag
      901 tggcatcgcc ggtattgatc ataaagttgc agtgtttttt gctcatttgt gcgccgccaa
      961 ccctgaggcc gcgcgcgccg actttttcga ccacctgcca ggcgcgggtg tcttcgggca
     1021 atcccgcttt gcggagttcg gcaggcggag gattggcgaa ggttgaaccg ccggtttttt
     1081 cctttatggg ctgggtatca ttacgcttct ttttgatctc ggtgatgcgg gcctttaccg
     1141 tttcataatc ttctgcgacg cctttgagga tggcgctggt gaaaatcatc tcttcgggca
     1201 attcggtatg gcgatagctc attttgagat cttcgagcac gatacgaatt tcgtgaccag
     1261 cgcgggttat tgcattgacc ccgatcagca cgtcttttat ttccgagccg taagctccgg
     1321 cattcatgcg caatgcgccg cccaatgtgc cgggaatgcc ggagaggaat tcgaggccgc
     1381 ctattccggc tttcacagct gcggcggcga cattgccgtt tagcgcgccg cacccggcct
     1441 ggatgtagcg tccatccaga acttttacct ccgcgaaggg cttgccgagc tggatggtgg
     1501 cgccgcgaat gccgccgtca cgcacgatgg tattggccat gccgccgatg atagtgaggt
     1561 tgccccccca cgcgctttta cccccccacc ctaaccctcc ccccgagagg gggaggggag
     1621 ttttcgtgct cccccgaccc tcccccggag gggatttttt gggagtcagc ggccaaacct
     1681 tgagaaagac agcaagatca tcggcatcgg cgggttgaaa cagcagatcg gcatggccgc
     1741 cgcagcgaaa ccagctttgc gcgcccagag gggcgttttc ggtgagagtg ccgcggattc
     1801 ccttcacacg tcattgcgag cacagcgaag caatccaact cttatagatg gattgccgcg
     1861 tcgggcttcg ccctcctcgc aatgacattt tggaaacata gcttctaaca tactgttttt
     1921 actcaaaatt ctttatatcc ttcgctttgt ttttcatttt tgccggggtg ggggggcttt
     1981 cgctttttgg catgtttttt gtgttcgttt ttcttttaaa gattaggaaa aaattcttat
     2041 tttgtcaatg ccggggtaaa aattatgaac cacagatgaa cacagatcaa cacgggtatt
     2101 attcaattca ctgcgaaggc gcaaagacgc gaagcggttc atttgatttc gttcagtatg
     2161 tatcgtttct tgtccatcgc tggaatatag ccagaataaa tccagattac cagatccccg
     2221 cacaaggcgg ggatgacggg gatgacaatt gactgtggat tgcttccccc gccttcgcgg
     2281 gggcaggctt cctcgcaatg acgatacagc tatcaagctg catttccttt ggctttatcc
     2341 agttcggtgt caagctgggc gggcaggtcg taggcccagc cggtaatgtc gcccgcgccc
     2401 atgcagatga cgtagtcgcc ggggcgggcg aggcgcgcga tcatatgggc gagttccttg
     2461 cgatcgggca gcgccatgac ttcacggtgg ccgtggtttt ttataccttc gacgaggctg
     2521 cgtttatccg tgccttctat cgggtcttca ccggctgcgt aaatatcggc gatgatgaca
     2581 gcatcggcgt cgttgaagca tttgcaaaat tcgtcgaaca gattatgcag gcgggtatag
     2641 cgatggggct gcatgacggc aatgacgcga gcattatttt ccgccacggc catgcgcgca
     2701 gctttgagca ctgtttcgat ttcgatcggg tggtgcgcgt aatcatcaat gacggtgatg
     2761 tcgtggctga cgccggtttt ggtgaagcgg cgctttacgc cggtgaaacc ttccagcgct
     2821 tttttcatga tcggggcggc aacgttcatt tcgtaagcta tgcacagcgc ggccagcgcg
     2881 tttaaaatgt tgtgctgccc taacattggc aggtgaacat cgcgcagcat gcgctcatca
     2941 ggcaggctga catcaaacac agagccgtcg gggccgcttt tgacattgct gacttgtaca
     3001 tcggcctgag ggttaaagcc gtaagtgatg attttgcggt ctttcacgga agggatgagg
     3061 gcctgaacct ccgggtggtc gatgcacagc acggcgtagc cgtaaaacgg gacgttatgg
     3121 atgaactggc gataggcgtc tttgacagct tcaaacgtgc cgtaatgttc catatgttcg
     3181 gggtccatat tggtgaccac gcagacgctt gcgggcagac gggtaaaggt gccgtcgctt
     3241 tcatcggcct ccacaaccat gatgtcgctg gcgcccaggc gggtgtttgt gccataggca
     3301 ttgacgatgc cgccattgat gattgtgggg tcgaacccgg cggtctccaa catttgaccg
     3361 accattgagg tggtggtggt tttgccgtga gtgccgccga tggccaccgc ccatttcagg
     3421 cgcatcaact cggccagcat ttcggcgcgg cgaacaatgg gcaggccgag gtcgcgggcg
     3481 gcttgcagct ctggattggt tttcttgatg gcggaggaaa tcacgactgc ggcgggcaag
     3541 ttgccatcgg cggctttgag gttttcaggc gcgtggccga ctttgacttc aatgccggtt
     3601 tcgcgcaggc gctttacgtt cgggccctcg gccatgtcag aaccgcggac ggtgtaatcc
     3661 agcgaattca ggatttcggc gatgccgctc atcccgatgc cgccgatgcc gatgaaatga
     3721 agcgcgccaa tgtctttggg cagtccgtgt tgcatgtttg tttcctttta accacgaatg
     3781 gacacaaata aacacgaatg gttctagcaa aaaccataag gtgagacaaa ggaaaacact
     3841 gttttaacac tgaaaacaaa aaccccggaa cgtttccggg gtttcatggg atgattttta
     3901 tattttaccg gacatattca taacataggg caacagggac agagtctaat gcgaccacta
     3961 cttgtccgtt aggggatgct ttgagcctct ctggtgagac ttttttcgag gcaaaacagc
     4021 ctgttatccc gccaaattca aaattgtagc cttgagggtt tttataatat ttcatatccg
     4081 cgcaatcggc gtcatcgacc agatgagcga cgccgcttag ggtgtttgtt atttcattaa
     4141 actttaaaag tgtttccttg cacgcaagct ctccgtagtc aggagcaaga tcaatgttga
     4201 catatgtatt cgcgattaaa atcctgttgg tatctgcatc tgctgcaccg gcatggtaaa
     4261 ggttgtctgc ctctacctca ggggatcttc ccacaccttg agcttgagct tgttcccctt
     4321 ctattgcaat gctcgcttcg gtactgattc caacatctgt atcctgtttg acctttcccc
     4381 cgtaaattga gaacgcatcg ttatcgtaag aaggaatgcg gactacgcgg tgatcaatag
     4441 cgataatttt ctccgcacct tcaggatttc caaaagcaat cgtcagctct tgccctttat
     4501 ctccgtataa agatgttgca cagccggaca gttgtgttgc accaaatagc atagacagcg
     4561 ccataactct atttttcaac gacataatat tctcctcgcc actcaaaaac tgaggatgct
     4621 tttacattgt tattaaatgt tatgtcaata tttttatgaa aactactcat tccagccgct
     4681 catgatggcg gtgacgaggt tgccgagttt tcgcgcggcg tcggggcggc cacattcgcg
     4741 ggccttttcc gcagcgctaa acaatatttg cggattttgc aggaaagttt caatgcgcgc
     4801 aagcagcgct tcggacgtaa agccgctttc ggccatcacc cagccgccgc cggcatcgga
     4861 gacgatttcg gcgttcattt tttgttgctg gtctttatgg tgcggatagg gcacgaaaat
     4921 tgccgggcgt ccggcggcgc tcatttccgc gacggtgctg gcgccggaac ggccaataaa
     4981 caggtggcaa tgctccaatt cttcggcgac attgtcgatg aacggggaaa gatgcgcatt
     5041 aattttggct tcgtcatagc gcttttttgt ttcttgtaaa tcttcctggc gacattgctg
     5101 gactatatgc aggcgcgcac ggtgatcggc gggcaatccg gcaagcgcct tgggtaaaac
     5161 ttcactaaac acatgtgcgc cgagggatcc gcccatcacc aaaatgcgta attccccgtc
     5221 atggtcgagt ttaggatagg gctttgtata aagtgcagcg atctccgggc gcaccgggtt
     5281 gccggtaaag atggtgtgga ttttttcagc ctcttccagc ccgcgtagcg acggcgttga
     5341 cgaagcaatg cgcgcagcct ttggtgccaa catttcattg gcttttccga taaccgcgtt
     5401 ttgctcgtgc aagatggtgg ggattttcat cccttgggcc gcatagacag ctgggaagga
     5461 cgggtagccg ccaaagccga cgacgattgc gggccgggtt tttttgagca gagcgcgagc
     5521 ctgtaaaatg ccaagcccca gatcaaatgc gccttttatt ttgcccttaa tcccggcgcc
     5581 gagcgttccg gcttttattt cgtgtaaagg gatgtcttta aacagctcgg catatttcat
     5641 gccgcgcgaa tccgtgacca tctcgacgcg atagccgcgc gagatcagat caagcgccag
     5701 agcctgagcg ggcatgatat gaccgccggt gccgcctgcg ctgagtatta tcaatggatc
     5761 actcatggcg cagccccttg tgtcttggag gttgccagcg acagcccgcc cttggaaatg
     5821 ccggtgcggc gactacgccg cgtgagcgcc agcacaatgc ccatcgaaaa accgacggat
     5881 agcaaggacg agccgccata gcttatcagc ggcaaggtca tgcctttggt cggcaggagg
     5941 tggaggctgg agcccatatg caccaaggcc tgcagaccga acattgtgag caacccgccc
     6001 acggcgagga ccacaaacat atcatgcgaa tccatcaacc ggttatagcc gcgcagcaag
     6061 ataaatccgt agacgccgag gataatcagt acgaagacca gccccatttc ttcggcagca
     6121 acagaaaaaa taaaatcggc atgcgcatcg ggcaggccca tttttacggt cccctgccca
     6181 ggtccggtgc cgaacaagcc gccattttgg aaggcctgca aagatttatc gatttggtag
     6241 gtatcaccgg cttcgggatg aagatagcga tcaatgcggc tttggacgtg gtcaaatgcg
     6301 gcataagctg tgacaagacc gccgactccc aaaaccagca aaaccgcgag caggcgaaaa
     6361 gggaaaccgg ccagaaaaat ctgcgcggcc caaatggcgg tgacgatcac ggtcatgcct
     6421 aaatccggtt gaagtagcaa caatgtgatg gtcaggaaat acaggccggc ggtgatggcg
     6481 ttgccgggga atttttcctt ttccttttgc atagccataa accaggccgc cagtacggca
     6541 aaagcgggct tgataaattc gctgggttgg agagaaaaac caaaaatatg aatccagcgc
     6601 tgcgcacctt taatctccat gccagtgacc agcaccgcga tcattgcaat ggcactgccc
     6661 acaaaagcga gcgcggccag cctgcgaata ttgcgctgac cgagaaaaga aaagccaatc
     6721 aacatgccga ttgccggaac cagaatgacg agatgacgtt tgagaaaatg gtaattttct
     6781 aaaccgatgc gctcggccac aggcgggctg gccgtggcga ccagtgcaat gccgaataca
     6841 gccagcgtta taagcgcggc cagcatagcg cgatcaacgg tccaccacca ctggccgaga
     6901 ggagattggt cggtgcgggc aaagaggctc atgccgcggc tccgtctacc gaggtttgtg
     6961 tttcatcaag tgcgttgaca tagtttgtaa aatattcacc gcgctcttcg aaggatctga
     7021 actggtcgta agaggcacac gcaggagaca gcattactac gccgccaccg ccggggcgtc
     7081 cgcgttcgga ctgcgccaga ttgtgggctt cgttgacggc gttttccaaa tccccgcaca
     7141 gcgtgtaggg taccgaatat tgatcaagcc agcccgcgta ggcgatcatt gcctcaccaa
     7201 ttaaaaatgc atggcgtaca ttcgacatga gagaagaaat atcctcaaga ttatcgcctt
     7261 tgggccgacc gcctgcgatc caataaatat tgcggtaagc cgagagcgct ttgctggttg
     7321 cttcaacatt cgtggcctta ctgtcattaa tgtacgggat accgttgata acgcgcgttt
     7381 gaaactggcg gtgcggcaaa ccgggaaatg ttttcattgc ttccagaata acatctggtt
     7441 ccaatgctac atcctgagag gcacacactg cgtaagcagc agcagcgttt tggtggttgt
     7501 ggataccacg cagggaagca aaacctttta tcgaaccgat ctcgcgagac gctcctttgc
     7561 gcgcatcaaa caagatgccg tctttgacga aaacaccgtt ttcaacagcg ccttcaaaag
     7621 atagagggcg gacgcggcgc tggccggttt cataagccat catacgttcg taaatccggc
     7681 ggcagggttc atcatcaagg ctgataattg cgaggccttc tcctttaaaa atgcgttctt
     7741 tggcggcggc ataatttttg aaattgccat gccggtccag atgatcgggc gtaatattca
     7801 gcagcacaga aatatcgggc gtaaatgtcg tgcataaatc catctggtac gatgaaattt
     7861 ccagcacaaa gaatccgtct ttttttggca aatccagatc aagcacggga cgaccaatat
     7921 taccgcccat ggcaacgctg caccctgcgc tttcaaggat ataaccgatc agcgcggtgg
     7981 ttgttgattt tccgtttgtg ccggttatgc cgatggtttt gcaaccatgg ttacagcgat
     8041 gcagaatttc cagatcactg ataatttcca tgcccagttt tcgcgcggtc atgacaacct
     8101 gatgcggcct gggataaaag agcggcacgc ccggagcaag aaccaacaac gcacactctt
     8161 gcaaattctt ttgatcaaga tgtgtaattt cagcgccggt ttcactggcc gcatcgtgcg
     8221 tatcggcaag atcgtcccag gccttcacct ttgcaccagc tttgaccagc gctttaatgg
     8281 ttgaaatacc agaaagccca agaccaaata cggctacggg tttgccatcg agtgtttcaa
     8341 tgaattttgt acaatcaatc atacgctatc taagcttgag tgttgccaag ccgaccaatg
     8401 ccaaaataaa tgcaataatc cagaagcgta taacgattgt gggttccgac caaccctttt
     8461 tctcaaaatg atgatgcaaa ggcgccatgg cgaagactcg cttgccggtg agtttaaaag
     8521 atacaacctg cacaatcacc gaaacggtct cgagcacgaa caggccgccg acaatggcga
     8581 gtactaactc atgctttaca atcaccgcga ttgtaccgag aacacctccc atcgccaatg
     8641 agccggtatc gcccataaag atcatcgccg ggggcgcgtt gtaccataaa aaccccatcg
     8701 cgccgccgat gagggcacca cacagaatgg aaagttcgcc ggtgccgacg acgtaaggaa
     8761 tgcgcagata ttccgaaaag acttcgttgc cgacgagata gctgatcatt ccaaacacgc
     8821 ctgcggcgat agcgaccggg acgatggcca gcccgtccag tccgtctgtc aggttcacgg
     8881 cgtttgatga tcccaccatt accagcaagg cccaggggat gaacagcaag ccgatataga
     8941 tgtagatgtc tttgaggaca gggatcgcga tatgctgatt aatatggccg ggcagcgcgt
     9001 tcataatcca tattgcagct atactgccaa tcaggatttg tgcggccagc ttgagttttc
     9061 cgggcaggcc cttagtgtta cgctttgtca gtttgagata atcatcgccg aaaccaatca
     9121 ggccatatcc gaccatgacc agcagcgcga tccagacaaa ctgattgcta agatccgccc
     9181 aaagaagcgt tgaaagtgtc acggagatca aaatcatcag cccgcccatg gtcggagttc
     9241 ctgcctttgc aaaatgcgtc tccggaccat cttcgcggat gggctgacct tccttttgtt
     9301 tggcctttaa ccagccaatc atgcgcgggg caatcaggaa gcagatgagc agcgccgtca
     9361 gaatcgctcc gccggtgcgg aaggtcagat actgaaacag gttaaagaga atgaactgtt
     9421 cggacagcgg gaaaaggaga tgatagagca tacagtgtaa aaccttctat tctttagtta
     9481 cccagcgacc ctagcgcatt tgaggaaccg cgcgcaacat gccagcaaag cgaatgcgaa
     9541 accgcgggcc gacatgctta aagatcataa aatctcctgg tgcaaccact tcgggaacaa
     9601 ttgcggcaag cttggcattg cgggttacgg cgcgcactgc atcggtgaca tcggcgacta
     9661 cgccaaaaga acgcccggtg taaatacagt tgagattggc aatccggcgc ggcaacgaaa
     9721 gcccttttga ggacttggtt ttattttcca gatcaatttc ccattcggag ggcagcgcgt
     9781 tttgcaaaat taccgtttgc tgggcgtttc ccgtgtcgac aagtccgccg accttaagaa
     9841 cagaatatgg tgttctgggc cggggctgga cactcacggt tacacctgcg aggcgcacgg
     9901 ccttccagtt taaactattg cttcctaaca ttatactttc ttttcttcct ctttacttgg
     9961 ttcttgatgt tcgagatacg caatggcttt accggcctct tcaacatcat cgaaatgagt
    10021 cgtatggctg gcaaaaatct gtccttgttc gtgacctttt ccggcgatga ccagcacatc
    10081 gccggtttcg agctgtgtga tggctgtagt aatagcttcg cggcgatcgg caatttgttg
    10141 tgaggacgga acactgctga gaatagcagc acgaatatcg gcggggtttt cgccccgcgg
    10201 attgtcatct gtaacaatag tcaggtcggc atattgccct gcaaccgctc ccatgaccgg
    10261 gcgcttggcc ttatcgcgat taccgccgca gccaaagacg caaaccagct ttccttttgt
    10321 atgcggacgc aaggccttaa gaatattttc cagcgcatcg ggcgtatggg cataatcgac
    10381 atagactgcc gccccgggat gaccgggcac gagttggagg cgtcccggcg caccttggag
    10441 tttttccaac gcttcaatgt agatttcttt tttcgccggg tcatttgcga tgaccagccc
    10501 aagagcgcaa agcgcgttca tgacctgaaa ctcgccgacc agtggaagcg tgatgtcttt
    10561 atgcgcaccg gtaattccca gttctaaatc ctgcccgtga ccggcgggct taacggatag
    10621 aattttaagg tctttaccat ggtggccgta actcaaaatt tttacgccgc gccgtttgca
    10681 gatttcctgc agcttttcga attcgggaat gtcggcattg agtacagcca caccgctttc
    10741 tggcagaagc tcttcaaaca ggcgggcttt ggccgtgaaa tattcttcca tggagccgtg
    10801 gtaatcgagg tgatcgtggc tgagattggt aaaaccggcg gcttcaaact ctacaccgtc
    10861 aagacgatat tgatccagcc catgcgaaga ggcttccatt gccagatgcg tcacgccttc
    10921 ggcggcgagt tcggccagcc cggcctgcag cgcaacagga ccaggcgttg tcatcgcgcc
    10981 agacttcaca acgccagcac agcgtacacc cagcgttccg agagaggcgg cattataccc
    11041 ggctgcagtc caaagctgct gtacaaacag cgtggtagag gttttgccat ttgttccact
    11101 cacaactgcg atgtgctcag gctggcgtgc ataaaactgc gctgccaatt tggcaagggc
    11161 ttgccgcggg ttaggatctg tgagcagttt gacatccttt acgcccggtg gaagctgtgt
    11221 accttcggga gcaagaatca cactcgcacc gtgctcaatc gcggcgttaa tataatcacg
    11281 accatccact ttgcacccgg gcagcgctgc aaacaggtag ccctttttaa ccgcgcgact
    11341 atcttcgctc agacctttga tggtggagac atcaaaattc atcaatgctc ctcctttgta
    11401 ctaacaaatt gtttcaagga ctcaccgaag tggttttctg gtgtattatc tcctgctggt
    11461 gaaatgccaa ggatagcggc catagaggcg attacccgcg ctacggccgg agcggcaacc
    11521 cagccgccag tggcgtaatt ccatgtatgt ttttgacctt taggttcgtc aaccataatg
    11581 aaaaccgcat agcgcggttc atccattggg aaaataccaa caaaggatga aatctttttc
    11641 ttgctatcat aacggccatc ggcaattttt tcggcggtgc cggttttacc accaacgcga
    11701 taacccttca cttcggcttt tttggctgtg ccgtcggtca caacaagcct gagcaattga
    11761 cgcatacgct gggcggtttc agccgaaaca atgcgaatat ctttgccagg ctcttcttca
    11821 tcgctttgct catcaaggac gaggctgggt tttaccaaat agccgccatt gacgatggag
    11881 gatacggctg tgataagctg aagcggcgtt gtggatacgc catgaccata ggaagcggtg
    11941 agtgtgttga cctcgcgcca gggatggggc acaagcggac gcgtaacctc acgcacctca
    12001 aaatcgagcg ggtctaacag gccaagatca cgatagaaac tgcgcaaagc atccgtgccg
    12061 acggcgcggc ccatcagcgc ggagccaata tttgaggaat acataaacac ttcgggaatg
    12121 gtcagaaccc ggtcttctgc gtgataatca tggatcgtat ggcggccgat tttaatcggt
    12181 tcactggcat caaaggtcgt gctcatcggc acatcatgcg cctcgaaaaa tgcggcggtg
    12241 gaaaaaatct tgaacacaga tccgagttca taagctccca gggtcaggcg attaaaaatc
    12301 gcatctttat cggcgcggcc cggatcatgc ggattaaaat ctggcaaaga gacaccagcg
    12361 agaatttcac ctgtggttac gtccatgatt gcgccggctg cgccgatggc ctcaaattct
    12421 tccacggctt taagcatctc acggcgcagc acatgttgga ggcgaatatc caatgttaat
    12481 ttaagatcgt gtccgccgcc gagatgtttg ttaaaactgc gctcaattcc ggcaagccct
    12541 tgcgcatcaa cattaccata gccgagcaaa tgcgcggcca gcgccccttg cgggtaaaaa
    12601 cgtttatagc cctcctcaaa ctcaagcccc ggctctccga tttgcaagac ctgatattgc
    12661 tggtcagggc ttacgttgcg ctggagccag acgaagcgtt tctggctttg cagcttttga
    12721 aggatatcgc catatttcaa gtcaggaaaa attattatca gctttttggc agctgcatcc
    12781 gcatcggcga ttaagcgtgg atctgcatag agcgaagcgg ttttgagtgt ggtggcaagc
    12841 aacgcgccgt tgcgatcagt aatgtcggcg cggcgcggcg tttcgccgtt tatatttagc
    12901 atattgcttt cagcaatctg cccgttatcg ggtgcaatgg catgcccttg aataatcgcc
    12961 aggtcgaagg cacgcacggc gaacaccata tagaccagga taaaaaaacc actcatcaaa
    13021 acaagacgcc cgcgcgccag atccagagcc gaactgcgct ctccttcaat tttgactgta
    13081 tttcgacgta aacgctggga aaagatcatg gcacaccgcc atttttgctt tgacccaatt
    13141 cattcagcaa atcgccaaag gctttttcct ttacgggctt attgtgccgc attgatggat
    13201 ccgcgcgctg tggcttttgc acggggatcg gtgcgctttc cttttcctgg ccagcacttt
    13261 gaaatgtgac aggctgcaag accggggggg ggaagctttc aacttccggc agcacaaact
    13321 cagagggaac agattcaggg agatgcactt cctcgctggt catttgatca ggcgatggcg
    13381 gcaccaaatc aaggaactcg ttagccaaac gctcgaggcg ttcagggcga ttgagtgttt
    13441 cccattcagc tttcagcatg cgtatttttt cttcttcacg ctgaactgat tgctccagcg
    13501 cctcgagacg ctcctgagca tgttggacat tttgcgaggt gtggagcagt acggcgccag
    13561 ccagcccgac cagggcgaaa attataaaag tacggggctt gagcagtttc atgatgctcc
    13621 cccgatatat tgagcgccgc gcagccgggc tgaacgcgaa cgcgagtttt cggcgatttc
    13681 ctgctctccg ggttttacag ccttgcgggt gaggagtctg agttttgcgg gatcgttttc
    13741 agtttgcggc aaatagcgtg agactgcctc agcaccggat tgagaacgga aaaagcgttt
    13801 gacaaggcca tcctcaagag aatggaagga tataacaacc atgcgcccgc caggcttaag
    13861 aatgttttca gccgcattta atgcgcgctc cagctcgccc agttcgtcat tcaccgcgat
    13921 gcgcagcgcc tggaatgtac gggtcgccgg gtcaatgccg tcacggcttt tggggacaca
    13981 acaacgaaca atttcggcaa gctggcctgt agtttctatg ggcgcggcac ggcgcatctc
    14041 aacaatgcgg cgagcaatct ggcgagattt gcgctcctcg ccatatttat aaatcagatc
    14101 ggccagctct ttttcgtcca tttgcgcgat cagatccgct gcgcctttac cgctttcggg
    14161 gtccatgcgc atatccagcg gcgcatcagc gcgaaaagaa aaacctcgct cagcctgatc
    14221 aagctggaag gacgataccc cgagatcaag cacgaatcca tcaaccttct caatcccagc
    14281 tcccgccaac aagctttcta catcgccgaa gcaaccacgc aggaagatta gacgcccggc
    14341 ataattcctg ctcaaatcct ccgcacgttt ggctgcatcg ggatcacgat caatcgcgac
    14401 gaccgtacaa tcggctgcat ccaaaatggc cctgctatac ccacctgcac caaaggtccc
    14461 atccacgtaa acaccgccat ccacagggga tagcgccgcc aaaacttcat ttagcataac
    14521 aggataatgg ctcaagcgct tgagtccttt tattgaatca aaacaatgtg ttacgcagcg
    14581 atgtgagaat cgattctgga ttcgaaaaga ggcttatgaa caagccttga ctcttttaga
    14641 agtatcagag gaagaagaga ttcgtcaatt tatccgccga gtcgcaccta gagttgtccc
    14701 catttatctt tttgtggagg aatcgttttt gtattttatt ctttcatttt caatgaaaaa
    14761 gagcgctcaa acgccgcgca atctttcgcg aaacggttcg cgcggcccgc agtcgtgggc
    14821 ttgataagag ataaacttgc gttttgcggt caaaatcggc atagtgcgcc cttcagaaga
    14881 tcgggcagcc gctggccacg ctttaaacag tggggcgaga ggaaagtccg ggcttcacgg
    14941 aaaaacggcg ccgggtaact cccggggggc gcgagcctac ggacagtgca acagaaagca
    15001 aaccgcctga ttattcaggt aagggtgaaa gggtgcggta agagcgcacc gcgtgcatgg
    15061 taacatcgca cggcacggta aaccccgccg gaagcaagac cgaataggaa cgcatgatgg
    15121 cgtttttccg cgccgtgttc gggttggtcg cttaagcgtt tcagtaatga tgcgcgtaga
    15181 tgaatggttg ccgctccttt gggagtacag gacccggctt atagattttc tgagctttat
    15241 gattggccgc cgcggggaaa ctccggcggc ctttcttctt tgtcattgct tcaccgccgc
    15301 tggcggttcg caatgacgga tgctgatggg ccagaaactt tattcagaat gaatattcgg
    15361 tacgccatgc tgatcaagcc agaaccaatc aaaaagctcg ccgggatctt gtttgcggcc
    15421 cggggcgacg tcttcgtggc cgagcacggt tttgatttcg tggcgggaca tgatgtcctt
    15481 gcataatttg gccaatgcgg ccatttgttc atgcggaaac gggtgatagc cgaattcatg
    15541 gcccgggttg accagttcga tgccgatgct ggcggagttg atatccgtac agccttccca
    15601 ctctgacacg cccgcatgcc aggcgcgttt atcctcgtcc acgagttggt agagagtgcc
    15661 gtcttcgtca atgacgtaat gggcgctaac ctcagcctct ggattgcaga gccgctgaag
    15721 cgcggcttgg gcggttttca tgccggtata atggatgaca atcatcgacg gcgcgcagtc
    15781 atcggcgcgg tcgttgtagt tgggcgaagt ataatcgttt aaaatcatgt aacggtttcc
    15841 aataaactta cgaattcgat tgtaacagat ccctgctttt gcggggatga caaaatggaa
    15901 agactatgaa atgaacaatt tttttgtgcc ggttttgatg tttgcgggcc ttgtggtttt
    15961 gctatggttt gcggccagcc cgcagcggag catggccgaa aaaggcttag gccgcgcgca
    16021 tttaggaggt gatctgatgg aaatgaaacc cgaaggctat ccggtggcga tttttgccgg
    16081 tggatgtttt tggtgcacgg aaagcgaata tcgcagccag ccgggtgtgc tgtatacacg
    16141 cgtgggctat attggcgggc atatggacaa tccgaaatat gaagatacgc atggttccaa
    16201 atccggccat gccgaggccg ttgaggtgac gtatgatccg gataagaccg attttcgggc
    16261 gttgctgatg tttttcttta cccaagccca cgacccgacg cagctcaacc gtcaggggcc
    16321 ggatgtcggg acgcagtatc gctcagcggt tttttatacc gatgaggcgc aaaagcgcca
    16381 agcgcaggag gtgattgatg agcttacggc ggcgaagcgg tttgcgaagc cgattgtgac
    16441 gacgctggag cccgccggca cgttctggga ggccgaggat tatcatcagc gctattacga
    16501 gaaatatgag gcaaaaaccg gccgcccgca tattaatgac tggctggcgc ggcaacggcg
    16561 gggggattag gctcccccct tatgaagtca ttcccgcgca ggcgggaatc cgccgctcag
    16621 ttgaaaactg gatccctgct ttcgcaggga tgacttcgca gagattaagc ggctttaacc
    16681 tgcccccggc ttgaacgggg gcggcaattc agccattatt tcaagagatg gattgcttcg
    16741 tcgcttacgc tcctcgcaat gacgttttga ccctagcccc gcttatacgg tacagatgtt
    16801 aacgggtatt caatgaccag tttatccgtt gcttcgttgt ggagattgag ggcctttatt
    16861 tcttccaaag ttgtatagac ctggccatgg aaaacaacga aaacattatg agagggcttc
    16921 aattcatgtt tcttcgccaa atcaaccgcg gtgattgcag acatgatatt cattcctgaa
    16981 tccgagaagg tgaaaccggt tggtaacatg ctccctgtta cggcaacaat tttgtcttga
    17041 acaacgtcgg tatcgattag gatctctgcg attttcgtga gcagatatgt tccgcatgtg
    17101 atcaaaatgc gctcattctc aatggaggat acaaaatcaa gcaatgcttc caagtccgca
    17161 ttttccaggt cgcggctgtc ttttgaggat agttctgccg atgcaaatgt atcggtgtta
    17221 atgccgaatt taccctgaag gtcctcaatc aaggttctgg aaacatggca tggaaacggg
    17281 aaaagagatt ctttattcgg attatagccc gacgaaattg taccgcccat accgataaag
    17341 gcgaagcggt ctttctcgtt ttcaaaatct ttgacgtatc tgaggatttt ctgcgcctgc
    17401 accttatcag gctttacata ggttttcttt aaagcctcta tatccatctt cattctggta
    17461 ttcatggcat ttccttcctg catagccaaa aatttataat atcagagtgc ttctcatgca
    17521 atctcaggcg gctttggcct ctttcaccac gtcggtgcag cggttgcaca gatcaccgtt
    17581 ttgagagacc tcgggcagga ctttccagca gcgggaacat ttctggccgg gggctttttc
    17641 gattttcacg gacggatcgc cttctttcac ctgaagatcg gaaacgatgc agatatcggc
    17701 catttccccg gctattgatt ttacataatg cgattgtgtg gagatggtgg ggagagcctc
    17761 gagggaggag ccgagggttt tatcggcgcg caggggttcg atggcttcaa gaaccgattt
    17821 gcgaattgtc atgatttcgg cccacttctc ggccaactcc gggtttttcc agctttcgga
    17881 gatagttggg aattcgcgca ggtggatgga ttcctcatct ccaaacactc ccgcagggcg
    17941 gtgggaccac gcctcttcgg cggtgaagac cagcacgggg gccagccagc ttaccaagca
    18001 tccgaagatt tcggccatga cggtgcgggt tgcgcggcgt tcaaaagagg ctggctcatc
    18061 gcaatagagg cggtccttgc ggatgtcgaa ataaaaggcg ctgagttctt cgttgcagaa
    18121 atcgtgcagg gctttggtca ggcgcatgaa gtcatatttt tcaatgcagg tgcgcagttt
    18181 ggtatccatt tcggtgagct ggtgcaagac gaggcgttcg agctcgggca cgcgttcgtc
    18241 gctcaaatcg acgcgttcgg cctcggtgaa gccgtcgagc gcaccgagca agaagcgcag
    18301 cgtgttgcgg atgcggcgat agaggtcgcc ggtggtttta atgctgtcct tgccgatgcg
    18361 gatatcttcg gagaaatcgc aagtcagcgc ccaaaggcgc aggatgtcag caccagattg
    18421 ctccataagt ttgagcgggt cgacgacatt gcccagcgat ttggacattt tatagccctt
    18481 ttcgtcgagc acgaaaccgt gggtcagcac ggtgtcatag ggcgcggttc cacgtgtgcc
    18541 gcagctttcg agcagggatg aatggaacca gccgcggtgt tggtccgaac cttcgaggta
    18601 gaggttagcc gggcgcttgc ctgccgggcg gcgcagatcg tcgcggtctt cgcagacaaa
    18661 ggcgtgggtg gagccggact caaaccagac atcgacgatg tcgaagattt gcgtgtaatc
    18721 ggcggcgttg tagtcattac ccaagaaatc ctgcggattg cgcgaccacc aggcatctga
    18781 accttcggcc tcgaaaatat cgccgatgcg gttgaggacg gtttcatcgc gtaagatttc
    18841 gtcggtttct ttatgcacga acagagcaat cggcacgccc cacgcgcgct ggcgtgagat
    18901 gcaccagtca gggcggcccg cgatcatcgc gcggatgcgg gcctcgccct tggccgggac
    18961 ccattttgtg tcgccgatgg cggtgagggc tttaccccgc agatcgtttt tgtccatgga
    19021 aataaaccat tgcggggtgg tgcggaagat cagcggggct ttggagcgcc agctatgcgg
    19081 gtattcatgt ttgagcgagc ctttggccag aagtttgccg gcttcgtcca tggctttgag
    19141 cacagcgaag ttgccgtcgc cgagcttgcc gtcttgcgtg tagacctcaa gccccgcaaa
    19201 aagcggaacg tgggggcgaa atttcccatc atcggcgaca ttatcggtga cttctatgcc
    19261 gttggcggtg ccgagataaa agtcgtcttc cccatggccg ggggcgatgt ggacaaagcc
    19321 cgttccggca tcttcggtga cgaaatttcc aaacagtaaa ggaacatcaa aatcgtagcc
    19381 ttgcccatga agaggatgtt ggcaggttgt attataaaac agacctgaac cttctccgtc
    19441 acctacagga ttaaggtttc cccatgctct taacttggac catctaataa ttttagcttt
    19501 ttcttttaca tcctctgcga gattttcggc taagagtaac ctttcaccta caatagcacg
    19561 gctgtcttcg gctacttcct ctacttcata tagcgcataa tggatttcgc caaaggcgat
    19621 ggcgcggttg gagggcatgg tccaaggcgt agtcgtccag atcacgacat tagcaccctc
    19681 caataatggc tcgttggggc aatcaataat aggaaactta atccagatgg tgatggattt
    19741 gtgttcttta tattcgactt cggcctcggc cagtgcggtt ttttcaacca ccgaccacat
    19801 caccggcttg gcgcctttat acaggccgcc gttacgcacg aattcatgga tttcgcgtac
    19861 gattttgcct tcggcgcgct tggtcatggt cagatacggg tctttccaat cgccgctgac
    19921 gccgaggcgc tgaaactggg cgctttgcgt ctcaacccag ccctgggcga atttgcggca
    19981 ttcatcgcgg aaggccagtg gggcgacctc gtctttgtcc ttgccggcgt tacggtattg
    20041 ttcctcgatc ttccattcga tcggcaggcc gtgacagtcc cagcccggca cgtagggcgc
    20101 atcatagccg agcattgaat aggatttcac gacaacatcc ttgaggattt tgttcagcgc
    20161 gtggcccata tgaatatcac cgttggcgta tggcgggccg tcatgcagga cccagcgctc
    20221 tttgcctttt gtgttttcgc ggatttggcc atagagatcc atgtcctgcc attgttgcag
    20281 gatttcgggt tctttttgcg gcagcccgcc gcgcatcgga aagtcagttt tgggcaggaa
    20341 caccgtgtct ttgtatttgt ctttattgtc gctgtcttta ttcatttttc ataatccgct
    20401 ttctatcggg ggcgggtttg tgatggtgtt tcacgtttta atgttgatgg attgtatatg
    20461 caagaactcc cggtctctta agtcagagcc gggatgataa ttcggataat tatggcgttt
    20521 tctgcgtgca tcggcgcact atagggtaat tactgcgggc aaatcaagca cggaaattta
    20581 ttagaaattc aatggatacc catcaaacgt catggcaaaa ggattggctt ttttctgcat
    20641 attcggtgat gcagtgctcg catattggcc gggataggtc tggccactcg acagcggcat
    20701 tccatccatc attggtcgcg ttactgtttc accctccatt ccttgtgcag cttgaggaaa
    20761 agctgcttgt cttaagggaa ccgccccagc ttgcgctgtc atcatttttc tccgtaaaat
    20821 ttaccgttgg ccgtcaaaaa ttccgaggac tatgaatcaa aagggagaac gggtcaagcn
    20881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gttggccgtc aaaaattccg
    21001 aggactatga atcaaaaggg agaacgggtc aagctttttc gggcagcttt cttaccaccc
    21061 ctcatttttt caaaaacgtt agttttccgt cattgcgagg agccataggc gacaaagcaa
    21121 tccaggaaat ccgggtgttc tggatcgccg cgctccctct ggtcgctcgc gatgacgaaa
    21181 ttgaggggta agagcaattt tgctctatcg tcacacagaa actttatggt aaattctggt
    21241 gtttaaaggc cggaaaccag cagaattaga gggatgtaaa acagggcgaa aatggtgccg
    21301 atgagaatcg ttgtggccgc tttttcgggg ctcatgtcga gctttgcggc aaaggcggcg
    21361 atattggcgg cgggcggaac gatcgccatc aggaagacca ttttgtgaat ttccgggcca
    21421 aaccatccgg tgacgccttg atcgaacaag atcaggccat aggccgctgc gggccaaatg
    21481 atgaattttc cgacaaaggt cagcgccaga aatttcgggg cgatgatcag cttttccgct
    21541 ttggccagag aggcgccgat aatcatcatg ccgaccagca cgtaagcgcc tttgaaatgc
    21601 tcccagtaat tgttaaacag ggccgggagc tccgtgcccg ccatattgac gccaagtcca
    21661 gcgattacgg cataaatact ggggaacttg aaaagcttga tcaggctgtc gcgcatattg
    21721 aagcgaccgc gattggcgat gtaatacatc accgtggctt cgtaaaccaa tccgccgacc
    21781 agtgcgaaaa tgtaaacgcc gacccattcc ggatcaaaca gcagcaggac tatcggcagg
    21841 ccgaaatagc cggtgttccc tgccccgccg cacatcgcca gcaaattggc gcggtggtcg
    21901 tcatatatcc tttgcgccag catgtaataa agcagggtca gccatgaaaa cagcgcaaag
    21961 acgatgatcg gcagaaggat gtaaaccggc tggagttcga tttttgcgac aaaaccgaaa
    22021 gcgacgaccg gcatgaggat gtatatgccg agactgccga gactttggcg atcgacatcg
    22081 aaatagcgtc cggcgatcca gcccaggcca atcagcatat aaagcgggat gagattggtg
    22141 aaaagtgtgg tgaaaatgtc catgtttttt atcttttctt cttaatgctt atttccgtta
    22201 aaaatctgac atattcttct tatagactgg acacccccac ccctgccctc cccctcaagg
    22261 gggaggggtg tggatggaga ggatttcacg ggcttgggcg cagtcttttt ccatctggtc
    22321 gatcagggcg tcgagcgagg tgaatttggc ctcgctgcgc agacgttgaa ccggccggat
    22381 gcgcagtgtc ttgccataga tttcacggtc aaagtcgaaa atgaagcttt caacctgcgc
    22441 ggtcggaact tcaaacatcg ggcgaatgcc gatattggtg gcggcattat gccactcttc
    22501 ctccccttcg atttgcacat gcgtggcgta aacgccatag gccgggtgaa cggtttcatg
    22561 cagggcaacg ttggccgtgg ggtaaccgag ctggcggccc cgttgatcgc ctttaacgac
    22621 ccgtccgcgt atttcccagt tccagcccag gttttcattg gcagcaccga ggtctccgat
    22681 gcgcagggcg ctgcgcacgg cgctggagga aaatactttg ccgtcatcac cgacaatttt
    22741 ttcaaccgtt gtgagtgcga tgcctgcgtc cctgatatcc tcaggctctc ccttgcggag
    22801 ctggccgaag cggaaatcct cgccaatgac gatatgatgg gggtgcaggc cgcccagcaa
    22861 aacaccggtg ataaaatccg ccgcgctttg cgaggcaaag tcccagtcaa agggtaaaga
    22921 cactacaaaa tcaacgccaa attcagccag acgccagtgt ttcacttcgg gtggtgtaat
    22981 acggctgggc ggatcgtcag gacggaacaa gcggcgcgga tgcggttcga aggtcagcac
    23041 gccgaagctt ttcccttcgc tgaccgcgat gtcttttgcc cggcccaaaa gcgcctgatg
    23101 cccgcgatgt acaccatcga aattaccgat tgcgatgacc gcgcctttgg ccgaatccgt
    23161 tatattttcc aagcgttcaa aaacttccat gatgtccgtt atgaaatgtt tttgattgaa
    23221 agtccatcta cggactcgct ttttttataa aagcttgtaa cctttcatct gctgcgttcg
    23281 aagacacgac catacgaggg ggacatccct ttgcatgaac aaacctaacc attgaaggag
    23341 atatattaaa tggtttcgaa gaaagctgta aatacactct tggcttctgc tgttgcgctt
    23401 actgttgccg gtggtatcgg tgctgccaac gctatggatg aaggcacaga gaaatgcttt
    23461 ggcgttgtaa aagccgggca caatgactgc ggcaatgccg ataagacgca ttcctgtgcc
    23521 ggacaggcgg ccgaagatgg cgctggcaat gagtggattg ctctgccaac cggtatttgt
    23581 gagaaattga tcggtggttc tttggagcca acatccggca gcgcagatga cgtgatgcat
    23641 gatgaagata tgcatgaaga caacatgcat gatgaacatg aaggcgaagg ccactaagat
    23701 atgacgtcgc atagcacgat tacgaaccgt gctgatcaac tattcccacc ggctaaaatt
    23761 ccttatgaac cggtgggaat tggttttcgg acaccccatt atgaagaggt ccttgagact
    23821 gatttaaatc tgggatggct ggaagttcat cctgaaaatt attttggcgg tggtgtgcat
    23881 cgccattttc tttctgaagt gcgcaggaaa tatcagatca gtctgcatgc ggttggcctg
    23941 tctttgggtt ctgaccagcc ggtttcggaa gatcatctgg cgcgtgttcg cgagctcatc
    24001 gatatttatg atccgtttag cgtgtccgac catgcgtcat ggagcgcaag cggcaatgcg
    24061 catttaaacg atcttcttcc cctgccctat acacaggcca gtctcaacaa gttggcgcgc
    24121 aatgttgagc gcgcgcaaga agcgctgggg cggcggatgc tgattgaaaa cccttcgact
    24181 tatctggcgt tcaaaggcaa tgagatgcat gaagacacgt tcatgaataa actggcagac
    24241 atgacggggt gcgggatttt gctggacctc aataacatct atgtgcaggc gcataatcac
    24301 gattacgatg cttggagcta tgttgatatg attgaagcgc accatgtcgg cgagatgcat
    24361 ctggccggac atattgaaca gacggccgga gagggaacgc ttttggttga tacgcatagc
    24421 cgtccggtta aaggggatgt gtgggggtta tatgaacatg ccatcaagcg tattggcgtc
    24481 gtgccgaccc tcatcgaatg ggacaacgat atccccgatt tggcgacgtt ggtgagcgag
    24541 gcggcgaaag cacgggcgat tatacaaaaa gtacgcgcag aagatcttcc cgatgcagcg
    24601 gaataattta aatacgtcat tgcaccctct ggggcataaa cttcgcgaag caatccaaat
    24661 atgtggattg ccgcgtcggc ctatcggcct cctcgcaatg acaagacagg aaatacagtt
    24721 caatgcggct gaatgaattt caaaaacgct ttaaggattt aatgctagat cacccggatg
    24781 ctttgcatgc gccgccggag gatctggtgg atttttgccg gtccggtgat attgcacttc
    24841 ctgagcggct gaaagtgtat cgcaataata tcgtcggcag tctcactgac gttatgctgg
    24901 caacatttcc ggtgatggaa aaattggtcg gcaaagaatt tctggaaatg atggcgcgca
    24961 gctttattct taaaaacccg ccgaaccatg gttgtttaag cctttatgga gaggggttcg
    25021 cagaatttat tgaggggttt gaactggcca aatccctacc ctatctgcct gatgttgcac
    25081 ggtttgagtt ggcgctaaac agcgcttatt acgcagcgga tgacaggcct ttgaccgccg
    25141 aagcgctggg gcggattgcg cctgaacatc tggagaacct gaaacttgtc cttagagaca
    25201 gtgtacaact tttgcgctcg cgttatccgc tcagcgcgat tcaggatttc tgtatgagcg
    25261 gtgcgcaaga gggtacgctc aatcttgatc aaggtggtga gcacttgatg gtttttcggc
    25321 ccggtttgaa tacgcaaact gtcttgctgg atgaggatga gtatctgatg ctggtactgt
    25381 tggctgacgg gcagacgctg ggcgcggcgg ttgaaaaagt tttggagacg catccagact
    25441 ttgattttca aaatttctta caaaaacata tgtcgcttga aacttttcgt gctctttaag
    25501 cacacatttc cctaataaat tgtattttgg tcattgcgag cggcagcgaa gcaatctaaa
    25561 gtctggattg cttcgtaagc cttacggctt tctcgcaatg acgacttaag tgggaccatg
    25621 caaaaatttc cttacctgat ccttgcgctt gttatcttta cgactgacca aatcagcaaa
    25681 tggtttgtga ccgaagttat gatcaaggtg gtggttgaaa aacccatgcc gagtacgcta
    25741 ggcttttttg agtggctttc ctctgctccg gcaaggctgc ctcctgtatc gatagaaatc
    25801 ctgccctttt tcaacattgt gatggtgtgg aatcagggtg tgagttttgg catgttcagc
    25861 aatggcttgc attgggggcc atggtttctc agcggtttat cggtgataat ttcgattggg
    25921 tttttgattt ggttgtcgcg cagcttctca cggctgcaat ccgttgccat tgtgatggtt
    25981 attgccggag cactaggcaa tgtggtagac cggatgcggt ttggcgcagt gattgatttt
    26041 ctggacgtgc acgcgctggg ctatcactgg ccggctttta atatcgccga tagcgctatt
    26101 tgcatcgggg tttttttgtt gatcatacag tccttctttt ttgaaactgg tggaaaaaat
    26161 gctaaataac aatcatggca aatgtaagag tgacaaaatg aagagtttta acactatttt
    26221 tcttgtgctt gtggggctgg tggcgcttag cgcttgcgaa accgcgcaag agcagttggg
    26281 ccttactaaa gaggctcctg acgaatttaa ggtcgttaaa cgcgcgccat tagaaatgcc
    26341 gccaaactat accttgcgcc cgccaagccc cggtgcggcg cgtccgcaag aacaggaaac
    26401 ggcagaagag gcgagaaaaa cggttttggg taatgcgtcg gcgcaaaata cgtatacgcc
    26461 cagcggttca gaggccgctt tgctgcaagc agccggagca acacaaaccg atcccgctat
    26521 tcgccgcaaa gtcgatgagg aaacggccaa tatgagagat gaaaatgagc cggtcattga
    26581 gaaaattatg agcatcggca gagacaggcc accgcctgca aaagttgtcg atgcggccaa
    26641 ggaagcgcag cgtctacaga aaaaccaagc ggagggtaag ccggtgactt ccggtgaaac
    26701 cccttctgtt gaataggtaa ggatttcaag agcatgttct attctttaag gctttgtaag
    26761 tctttgcttg ttttgagcgt tcttgttgcc agcatcttgc ccttcacggc gcttgccgag
    26821 aagcaagaac taccggatga acgcgccgcc acgcctgatg cgccgcagga agaacgccgc
    26881 agggttttca atgctgaaag ctttacgctt gataatggta tggaggttgt ggtcattccc
    26941 aatgaccgcg cgccggtggt tacacatatg gtgtggtata aattcggcgc ggcggacgag
    27001 ccgattggca agtcaggcat tgcgcatttt cttgaacatt tgatgtttaa gggttctgcc
    27061 caagttggcg ggcgcgatct tgatcccgga gagttttcag aaatcatccg ttccatgggc
    27121 ggaaatgaca acgcgtttac gagccaagat tatatttcct atttccagtc cgttccggcc
    27181 caacatcttg aaaccgtaat gcggatggag gctggacgga taaacgatat caatccgcca
    27241 gccgacgaag tcctgtctga gcgcaaggtt attttggaag aacgcaaaca acgcacggat
    27301 aatgatccgc gcgggcaatt tggcgaacaa ttggcggcgg cggcgtttgt aaaccaccct
    27361 tacggcattc cggtgattgg ctggaagcat gagatggatg agctgtctta tgaagatgcc
    27421 aaagcgtttt atgatcgctg gtatgcgccg aataacgcga ttttggtggt ttcaggcgac
    27481 gtgacgcccg gcagggtttt tcagctggcg attgatattt acgggcagtt gccgcgtgag
    27541 gatgtgccta aacgtgaacg aacccgctca cctgcgctca attcccatac cagcgtaacg
    27601 cttgagcacc cggctattcg tgaaccgctg gtacaaacgt tgttccgcgt tccatcggcg
    27661 cgtcaaaaca aagaagagtc tttggctctg caggttttgg aagaaatact gggcggtgga
    27721 ccaacttcgc ggctgtataa atccctggtt gtcgatcaaa aaatcgcgtc gagtgccggg
    27781 atcagctata actcagctgc atgggatgat tccagcctgt ggatttatgc gacgccattg
    27841 caggggaaaa ctctgagcga tattcaaacc gcgttaatgg atgagctgcg caagctcatt
    27901 aaagatggga tgcgcgaaaa agagctgggg gacgccaaat cgcggatgag cgatgaagcc
    27961 gtttatgccc gtgacagttt gaccggcccg gcgatggtga ttgggtacgg gctggcgaca
    28021 ggccaaagcc tggacgatat tgaatattgg ccctatgaca tcagcgaagt cacagctgac
    28081 caaatcaagg cggtcgctga gaaatacctc aaccccgatg cgccgggcga gcatcccccg
    28141 gtgagcggtt atttgttacc gcaggccgaa ggagacaaaa aatgattaca cgtttgtcat
    28201 tgcaaggagg gcaaagcccg acgcggcaat ctaaacttgt tttctatcct aaagggcaca
    28261 aatggattgc ttcgctggcg ctcgcaatga cattgttctg ggtgatgccg gcgcaggctg
    28321 aggacggtct gcttgatatt caggaggtta ccagtgaaag cggcatttct gcctggcttg
    28381 tggaagatca cagcataccg gtgattgctg tgaaattcgg ctggcgcggg gcgggatcgg
    28441 cgcgcgaccc ggaagataag caaggccttg cgcgtatgct gtccaatacg atggatgaag
    28501 gcgcgggctc tctcaattca caggctttcc aaaaagaatt gcgcgatctg gtgatttctt
    28561 taagtttttc cgccagccgc gatgatttta ccggctcact gaaaactctt accaaaaaca
    28621 aagaccgcgc tttcgagctt ttagagatgg cgctcacaaa gccgcgtttt gataaagagg
    28681 cggtggagcg gatgcgccaa gccaatcaga gccgcattcg ttcttccctt tccgatccgg
    28741 gctggatggc ggcgcgactg ctcaacgata aagcctttga gggacaccct tatgccttga
    28801 atagcggtgg aacgctatca tcattagagc ggattgagtc tgcggatctt aaaaagctgc
    28861 ataaaagcgt tttgggacgc aataacttga gcgtcgcggt agcaggcgat attacagccg
    28921 aagaactcgc tggcgtttta gaccaggttt tcagtgattt gccggatatc cttttggatg
    28981 accttgccaa tctggaaata caaaacgccg gaaagacctt tctttacaaa cgtgatattc
    29041 cacaaagcgt ggttgaaata ctacagcccg gcattggccg cgatgatcca cgtttccaca
    29101 gtgcacaagt gatgaacttt gttttagggt cgtctggatt tggttcacgt ttgacggaag
    29161 aaattcgtga aaagcgcggt ttgacttatg ggatttattc ttacttttac gatcttcagc
    29221 acctaaaagg gctggccgtt tcaacatcga cggcgaatga aaacgtacct gagatgctta
    29281 agcttatcaa agatgaattt aacaaaatgg tctcaagccc gatcggtgag aaagagctgc
    29341 gcgacgcaaa atcatatttg attggctcgc tgcccttgtc gctcacctca actgacaaaa
    29401 ttgccgggct catgaactca ctgcagcttg acggccgacc ggttgattat cttgaccaac
    29461 gtgaaaaagc cattgaggaa accagtattg aggacataca aacgcttgca acggaactgc
    29521 ttgaccctga acagtttatc acgattatag tgggcgagcc cgaaaagctt aaagacgtca
    29581 tagaagtgaa atccttgccg aatgtcgaat agtcccgccc atatgcccgt tcatgagcgc
    29641 ccggaatggg aagtgcttca agcgcaccaa cgggaaattt cgaacatgca tatgcgtgat
    29701 ttatttgcca atgatccgca gcgttttgag aattttagcg ctaagattcc cggcctgcta
    29761 ttggattatt caaagcatag aattacagat gaaacgatga atgctctgct ggcgctggcg
    29821 cgagcctgta atattgaacg tcaacgcgat gctatgtttt ccggtaacaa aatcaatagc
    29881 tctgaaaacc gtgctgtact tcatacggcg ctgcgccgat ctgcggatga aaaaatcatc
    29941 ctggatggcg aagatattat gtcttttgta tataaaacct tgtttcggat gcgtgatttc
    30001 agtgatgctg tgcgttcggg cacatggaca ggctgtacag gcaggcgtat aaaaactgtt
    30061 atcaatatag gcgttggcgg ctcggatttg ggaccgcaga tggtttgcga agctttaaag
    30121 ccctacgcag aacgaatggc agagtcttgt gggcaaaccg ttgaccttgc tgtgcgcttt
    30181 gtttctaata tcgacgcaac gatgcttcat gacaccctgc aagattgtga tcccgaaaca
    30241 acattgttta tcgttgcatc caagagtttt acaacgcagg aaaccataac caacgcttta
    30301 ggggtcaagg cctggctgct ggatactctc tcagatgaga cggctatcaa gcgtcatttt
    30361 gttgcacttt ctgccaacga agaggccgcc atgcagttcg gtattccacc ggaaaatatc
    30421 ttcccgatga aggactgggt tggcggacgc tacagtttgt ggggcgccat cggtctatca
    30481 atttgctgtg cagcagggtt tgatgtattt caggaccttt tagccggtgc aagcgccatg
    30541 gaccaccatt ttcagagtac gccgctggat aaaaacatac ctgtaatcat ggctattttg
    30601 gggatctgga accgaaattt tcagggctat ccggcgcagg cgattctgcc ctatgcccac
    30661 catttacagc gtttttccac ttatatccag cagcttgata tggaatcaaa cggcaagggt
    30721 gttacggcgg acggacggcc tgtgagcgtg gccagcgggc cgatcatttt tggagaggtc
    30781 ggcacgaatg cccagcatac atttatgcag cttttgcatc aaagtccgga ggttattcca
    30841 gcggatttta ttatggtggc aaagcctgtc cccccctatg aaaaccaaca taaaaagttg
    30901 ctcgccaatg cgctggcgca atccaaatcc ctgatggaag gacaggaaaa ctcagaggaa
    30961 ccacaccgta attttcctgg aaaccggccc agttcctgcg ttgttttgga cacgcttgat
    31021 gcttatcatt taggattgct gctggcgctt tacgagcata aaatctttgt tcagggcgta
    31081 atttggggca ttaacagctt tgatcaatgg ggcgttgagc ttggaaagac caacgcaaaa
    31141 catattatcg atgcctttga aactcataaa aaaatagata cactcgattc atcaacggct
    31201 ggaattttgc gccacctgac gcagaaattc ataaaatttt agaggccgtt cctgtaaaat
    31261 atacactctg cgtacaaaca agaattaacg gcaggcaatg ccgtttttgc atgcgaagaa
    31321 ggcgcaaagc atgagagcaa gcattcctga tccgaatgaa cttactcggc ttgaccagtc
    31381 aaagctggat gaggcgttgc gtaaacacga catgtatttg cgtggccaga ttggtggggc
    31441 gcgctgcgtt ctcaaatatc acaacctatc agagctggat tttaagcaat atgacctttc
    31501 acaggctgat tttaccggtt ctttgctggt tgaggctgat ctttcatatg gcgtttttat
    31561 tagcacctgt ttttttgctt gcgatatgcg caatgcaaac atgcataatg cagatttttc
    31621 acgggctgat tttcgcgggg cctatgtcgc aggggccaat ttgtccggcg ctaatctgag
    31681 tgacgtggat ttgcgtgaag gcaaaatcat gaagcgcgat gacaaaggtg tattggaaga
    31741 ccgtaaacgc tcaggtggaa cgggttcacg cgcaattctc tcaggcgcta agatgagcga
    31801 tacgaatatg agcagggcgc gcgctatgtc tgctgatttt tccgattcag atatgagcgg
    31861 cgtcactatg accgatgcca atttgtccgg cgcgagtttt gaaggcgcga acctggcgga
    31921 tgcagatctg agcgggtcgg acctatcaag tacaaatatg cgccaatcca ttatgaccgg
    31981 tacgattctg gaacgcaccg aacaacttga tatggacgat acggcagcag tgactgaaag
    32041 agatatgggc aacaagttag agaatcttgg taaaagcctg cctgaattac tggaggaaca
    32101 tacgctctgg gtcgctacag ccggacgtgc cggacggcaa ctggatttga gcggttatga
    32161 tttgcgtgat gtgctggatc tgaagagatt tcctcttacc gccattcaat gtacgggggc
    32221 aaattttctc aatcaggatt tgcgcagcgc cgaattgcaa agcggcgtat ttgaccgcag
    32281 cgattttcgc gattgcgata tgacggaggc cgatttgcgt ggctcgagct ttaaatatgc
    32341 acaaatggca cgcgttaatc ttaccggagc gcttatgtgt ccgctggagt ttaaaaagga
    32401 tatgggccac agcacattgc gccgcgttga ttttagcggg gcaaattttc gtttttcaaa
    32461 tttacgcggg gccgatttgc gcgatgctat tttgatggga gtcgatctga ccaatgctgt
    32521 tcttaatgat tgtgacttgc ggcgcgctga ttttaccggg gcaatcctga gcggagtcaa
    32581 gatggaaaat tgccgtcttg atgaagctat cattgatttt aaagcgctgt aaaagttatc
    32641 cacttccccc ttttccctgc ttgcttaaaa acctgacaac cggcgtttgt gacgctagtc
    32701 ttcttttatg tctatacgtt atttaccgga aacgctgatc aatcagattg cggcgggcga
    32761 agtgattgag cgcccgtttg cagccgtgaa agagctggtt gaaaatgcga ttgatgcaca
    32821 ggcaagccgc atcgatgtat cactgcaagc cgggggtaag agcctgattg tcgtgagtga
    32881 tgacggcaag ggtatgggcc gcgtggattt gattgcggcg gtagatcgtc atgcaacctc
    32941 aaagttgccc ggcgatgatc tggtgcatat agagcatttg ggttttcgtg gagaggcctt
    33001 ggcctcgatt gctgcggtgg cgcgcctgaa cattcaatcg cgcgcggaag gcgcgggcga
    33061 ggcgtgggaa atttgcgtcg agggcgggca gaaatttgat ccaactccgg cggcgcatcc
    33121 ggaaggtacg cagattgaag tgcgcgattt gtttttctcc acgccagcgc ggctgaaatt
    33181 ccttaaaacc gaccgggctg aatttatggc ggtgaaagat acgctcacgc ggctggccat
    33241 ggcgaatcca gaaattgctt ttacgctgag ccatgatgga aaaatatcgt taaaacttcc
    33301 tgccgccggt gatgtgcaag aacggatgag cgcgatattg ggccgcgatt ttggtgagaa
    33361 tgccatgccg attgaagcgg agcgtgaagg gattcggctt tcggggatgg cgagtctgcc
    33421 gacatatcac cgcgcgacga cagggtatca atatctgttc gtcaatggac gggccgtgcg
    33481 tgacaagctg ttgcatggct gcattcgcgg ggcgtattca gatgtattgc attcggggcg
    33541 gcatccggtg gcggcgctgt ttatcgacct gcctgcggcg gaagtggatg tcaatgtaca
    33601 cccggcaaag gccgaagtgc ggtttcgtga tccaggtctc gtacgcgggc tgatcgtgag
    33661 cgcgcttaaa cacgcgatcc atgagcatgg cggacaggcg gcgagcagtg tctcgaccgg
    33721 gatgctgggg cgttttcgtc cggctggtag tggcaatgcc ctgccctgcc cttgcatcgc
    33781 ggatcctctg cacctgtgct ttcgtcttat gcttatggca acatggccga agcggtgcat
    33841 aatgcttata cgctgcagcc aagcgttcag agttcaatgg cggtcgatat ggtgccggcg
    33901 gcgcggatgg aagtgcatga agcccccgta gaaacagtgc aaaattttcc tttgggcgcg
    33961 gcgcgggcgc aaattcatga gaattatatc attgcgcaaa atgccgatgg gctggtgatt
    34021 gttgatcagc atgcggcgca tgaacggctg gtttatgagc gctttaaagc gcagatgcgc
    34081 gagcggggta ttgagaagca agggttatta tcacctgaga ttgtcgagct ggaagaaagt
    34141 gaagcggagc gtttgctgaa atttgcgccg gaattggcca agctggggct ggagattgag
    34201 ccgtttggtg gaatggctgt ggcggtgcaa tcggtgccaa cattgttggg acagcgagcc
    34261 gatattcagg ggttaatccg cgatctggcc gatgagatta cggaagcggg cagcgcgcag
    34321 gggctggaag agcggctgaa tgaaattcta tcgacgatgg cgtgtcacgg ctcggtgcgt
    34381 tcggggcggc ggatgaatgt ggatgaaatg aacgcattat tgcgccagat ggaagctacg
    34441 ccgctttcag ggcaatgcaa tcatggacgc ccgacctctg ttgcactatc tttaaaggat
    34501 attgagcgtc tattcgggag aagatagctt gaatgaacaa ccacaaagtc acggtcggaa
    34561 atccgttttg aatttgtatg ctgcgctggg ggcaagcctg attctttcgg tcctgccttc
    34621 tgtgacggcg gcagggttat cgctggtgtt ttttctgggt gtattgattg cggcttatca
    34681 tatgcgcggt aaagttgaag aacacagcct gattgaaaat catgcgactt ttattatccg
    34741 cacgctgtgg atcggagcat ttttgacgct gccaaccatg ggactggcaa cggcctatat
    34801 gatgagtggt attgattacg cgccatttga ggtgtgcgcg caggcgctgg cgaataaggg
    34861 tgttgcgtgg atggaaagcg ccgggatgat ggaagtttac ggccttgttg agccttgcgt
    34921 tgagggtttt attactgaga ataaagtgtt gtttatcaat gcggtggtga ttgccggagc
    34981 gccggtggtc atctatatga cttatcggct gattaaaggg ttaagccggg ccagcaaagg
    35041 ctatcggctg gcgaagccta aagggtggtt ttgattttaa aattagaatg ccctgtgagg
    35101 atctggagtc tgcaattctt cattgacacg tttcagaacc tcgtttgcct gctctagctc
    35161 tgacgctgta acaccctccg ggacttctgc gctaccacag tccttttcaa ttacataaga
    35221 tccatcactc gcaaaaatca ttcccagttg agtgagctga caattttcac gaaacaattc
    35281 ttgaaggttc gattgtgcgt ttacacctac ctccccctta attacaagat ctgttccctt
    35341 ttcataccct gtatcttcgc tcatcacact ctccaaatct ttaaaagata ttagatttag
    35401 agtgtaccgt attatggtta attaattttt aatttctggc ggtggcgcga acatttaaag
    35461 caggttcaga aaggcggcag cggcttcgag gtcgcggagc atggcggcgt ctttcttttc
    35521 ggatagcgga tcgcggccat atttttccgc attgtaaatt tcatctttga agtgttcttc
    35581 gacgcgcgcg gcgttgtaaa tctcttgcgg ggtgattgcg ccttcgataa aggcgaggcc
    35641 gagcaccagc gaaccggata aaggaacaac cagttgtaga gcggtaaagc gggcatcgtc
    35701 cagcgcatcg gtctttgttt gcacggctgt gtgcgcggcg gctggttgtt tgagcgcagc
    35761 cagatccgtg gtggtttgca aggcgcaatc aaacttatct tcaaaccatg tgagccagcg
    35821 gtcccagctt tcggcctgct tttcggcttg tccggctggc tgatcttttg gtgcgcggta
    35881 gcagatcagg tctgtatcga ggaatttgag gagttcggcg ctcatcgcag cgcgctgctc
    35941 agacacccga tctatttgcg tgctgaggat ttgtgtcagc ggcattgttt cgggatcaat
    36001 ggtttcgcct tgcgcggccc attcttcctg gatagctgtc gccatagtct cgctggacgc
    36061 cagaagcgtt ttctttagcg gagtttttac cgggcggcta tcgagatgca ccgcccagcc
    36121 actgacttcc tgatgcacag agacaagttt ataaaatttt ttcatttatt gaggtccttg
    36181 gcttgcccct ctccggcttc ctgcggtttt gaatccggcg cttcgggaac agcttcgact
    36241 tcaggttttt ccggtgcggg caggaattct ttttcaatta tgaaagcttt gcagggatgg
    36301 tcttgcgcca aaccgaaatc agccagcgtc acggcataga aagccgggtt cacagcgcct
    36361 aaaagcagac caccgacacg cttgcctaga tcaaaaacat tcggggagac tttcagatcc
    36421 ggaagaggac cggatacatt aatggctgaa gcaatatcgc caatcgccac attctttggt
    36481 tttggttcca gaaccatttc aagctggtca gccttaaaat cgtaggtacc ctcaccataa
    36541 agtgtaatgc ttttggtatc gacaaagaca gagtcgctat gtcccttgag gtttttgaca
    36601 tcgagattga cgatgacgca gttcatattg agatcgctct ctttttcaaa gcttggcagc
    36661 aaagcattca gcaaaccgct gccccaaaga tcaagcagcc cggcgcgcat cttaccttgc
    36721 ccgccgacaa aaccaacttt tcccgaaagt ccttctatca aaccatccat gcttgtgctt
    36781 tgcgaagaca agtctattaa aatatcgccg tgaccttcga tagtgacttc accggtgctg
    36841 agttgttttt gaagagctct gtaatcaaag cctttgactg caattttctg tatcaatttg
    36901 gccgggcttt tggaggtcag caaatcgacg tggccactga gttctccccc cacgattttc
    36961 ccctccaagg ggccgatatg aagatctcct tcttctagga tcagcggggc ttttaatttc
    37021 ccaagctcga ctgccccgac cttaaatttt tgcacatcca gattcaagtc cagcgtcagg
    37081 ccactcaagt ccagagcggc gttttgctcg ccaaaaattt tatcaagagc atcaatggtc
    37141 ctttgcgcag gagattctcc agtaacatcg cttaagatta attcattgct ggttacttta
    37201 ccgaaaatca agggcgtttc acccgtccaa tccagagtaa catccggatc aatccgggtt
    37261 ttgcccggtt caaggcggag ctgcccgtta aactccaatt tcccggaact ggctcggtcc
    37321 atgtcgatat gaccatgcag cacttctttt tgtttattta gcaggacaat attttcaaat
    37381 ttatagccgc caataacggt attatgaatt gacccccgtg cggtcaaatc ggccaacgaa
    37441 atttcaaaat cgcgctggcg gccaattacg taggtatgcc ttccgggttt tccttttgtt
    37501 tgcagcccaa atttggcatg aagcggcgaa ttatcaattg ccccttcaac gatgaaggtg
    37561 gcccgcgcat cctgttcacg cacgttcata gcgcttactt ctatgctttt gttcagtatg
    37621 ctgcccggct tagccattag gttttcaatg tgaatatctt tgaaatgacc tggccggaac
    37681 agtgcatcga aaaagggcat caccagacgc gctgcaccga tgcggatcat tgtttcgccc
    37741 ataccatcat caccgccgcg cagctgtacg tcggtaatat caatggcaat attggggaaa
    37801 aaacgcatag aattgagtgt gccgatgcga gcggtatagg gcgtcgtgtc ggtgaggaat
    37861 tcctctatgg attcgcgcag aaccgggctg ttaccgccta ggcgcgagag cgcggttgct
    37921 aaaataaaca aaatcacgcc aatgatggcc agccatttta aaatgcgcaa gacgacagat
    37981 tcccagccct tatattccgg cacttggggg gcgttttctt cgctcatgat ttcgtatcct
    38041 caaacggatc ggccttcagg ctagggctaa agcctagcgc tttccaggtt tttttcaact
    38101 caggagggag tggcgccgtt acatcaagcg gagctttacg tccggaaggg tggggcagaa
    38161 taatgcggcg cgcgtgcaaa tggaggcgat cggcaaggtt gagcgccttg agatcggctt
    38221 cggccttgcg gcgcgcttcg tgggtttctt cctgttcggg cagacgggca tattttttgt
    38281 cgccaacaat cgggcaaccg atcaattcag aatgaacgcg gatctggtgg gtgcggccgg
    38341 tgcgcggcca aaaagcgaca aaggcggcgc tatgcaacgc gctttccagc acaatgaaat
    38401 cggtgatggc ggatttgcct tctttttcat cgacgaccat gcgctctttg ttattgccgc
    38461 ctgccttgat cagcggggct ttgatcgtgc cgtcagggcg gtcgggcgcc gggcatacaa
    38521 ccgcccagta aatttttttg actttacggg ttttaaatgc atcgcccatg gctttggcgg
    38581 cttttgcatt gcgggccagc aagaggatgc cgctggtgtc tttgtcgagg cgatgaacga
    38641 ggcgcggctt gacgccgtcc ttaccggtca gggcctccag cagcccatcg atatggtatt
    38701 tggttttgct gccgccttgg gaggctaacc ctgcgggctt attcagcgcg atcacatcgg
    38761 catcttcgta aatgaccatc gatttgatga aggctttatc cttatcggtg atttttactt
    38821 tctcatctat ggatttatgt tcgagtggcg gaatgcggat ttcctgaccg gctttaagct
    38881 tggcgtcagc ttttattcgt ttgccatcgg cgcgaatctg acctttacgg atcagctttt
    38941 gcgccaaccc atagggcagt tcgggcacgt tttttttcag ccagcgatcc aggcgctggc
    39001 catcatcatc ggctttgact tcgatttgcc ggacgttgct catgacacac atgctcgcac
    39061 aatggccatg gcagcgaaca acccggcgat ggacaggatt acggagccgc cgagatatag
    39121 cgcactgtaa agataggacc cgcgttccca caagttcacg aaatccagcg agaaggtgga
    39181 aaaggttgtg aaagcgccga gaaagccggt gacgataaac atgcgcatgg tttcggaagg
    39241 atgccagtaa tgggcgaata tggcgatcag caggcccata atgaaggagc caacgatatt
    39301 caccgttaat gtgccgtagg gaaagccatg gccgagcagc ttcaccgcgc ccacattgac
    39361 gccgtggcgc attacagcgc ctaaagcgcc tccggcggca attgcgagta ttgcgttcat
    39421 cgaaaattct ccttacaaag cattatcagg ttgtaatgga gttcagccat atgttcaatc
    39481 atcttcatcc atcacataag ggaatcgttt tagcctttat cggctttacg gcctttgcct
    39541 ttagcgatgt ggtttcgaaa tggctgctgc aatattattc gacctatcag gtggttttta
    39601 taaatcacag tacggcctgc ctttatttgc tggcggcttt acacatgtct gcgagcctaa
    39661 agactctgaa gcgcagcacc aaaatctctt tacacctctt tcgcggggtg ctgaatacct
    39721 gcgttgccct ttcggtgatt ttttccttct cgcgattgcc gattgccgat gtgtatgcgt
    39781 ttttattcac ggtgccgttt ttttcggcgc ttttgggggt ggcgttttac aaacaggcta
    39841 tcggcctgca tagaggcctt gcgattgtta taggctttac gggggtcgtt atcggtctac
    39901 agccgggcag cgaagggttt agtccatggc tgattgttcc tattttggca gggtttctga
    39961 ttgccctgaa atttacaatt tccagatctt tggaggggga aaacacctta ctgttgggcc
    40021 tttggccttt gctggtgtct gcgccgcttg caggactgct cagcatcagc agttttgaaa
    40081 tgctgaactt tacgcatttt gccatgcatc tgtttaccgg gctttgtatt gcaaccgggc
    40141 ttgtttgcgt ttcgcaggcc tttcggattg cccctgcggc cgccgtttca ccgattttgt
    40201 atacagaaat gatctgggcg ctgttgtttg gttttctgat ttttggagat gcgcccgatc
    40261 ttatgatgat ggtcggcgcg gggattatca ttttaagcgg gatttatctt gtcgaaacag
    40321 agcggcggaa tggccgcagc aatattaaag taaaatctta ataattgctt ttttataaat
    40381 caatacgata tgtttcgtat tgattgtaag ccttgctgag cctgtattaa accgtagaaa
    40441 ataccttagt aaagagttat agttgctctt tgagtatttt agaaagacaa caaaatggac
    40501 tggctgttta gtgtcgattt atggatgagt tttcttacgc tggtcgtact tgaaatcatt
    40561 ttgggtatcg ataacatcat ttttctttcc attgttgcat ccagactccc tgcagaaaaa
    40621 caaaaagcag cccgattgat cggcctagcg ttcgcccttc tcatgcgcat cgcgctgtta
    40681 ctcagcattt catggctgac ccacatcacg gtcattgtac ttagtattgc cggtcatgat
    40741 gtctcgtggc gcgatcttgt tttaggactt ggcggattgt ttctgcttta caaaggcaca
    40801 cgcgagattc atagcactgt agagggcgac gaccatgata cggcgaacat ctctaaagcc
    40861 gcaactttcg gcagtgtgat cacccagatt gtcatcatcg atatggtttt ttcccttgat
    40921 tccgtgatta cggccgtggg gatgacagat catgtcccgg tcatgatcgc ggctgtagcc
    40981 atcgcaatcg tgatgatgat gtttgccgca gagcctgcca gccgtttcgt tcaggaaaat
    41041 ttatccatca agatgctcgc cctcagtttc ctgctgctgg ttggggtggc cctgatcgcc
    41101 gatgcgatgc atttccacat tccgcgcggt tatctgtatt ttgcgattgc cttctctctc
    41161 ggtgtagaat cgttaaccct gctcgctcaa aaaagaagtg aaaagagaaa agccgcgcgg
    41221 gcatagaggt taagacttac ggcgtttttc ccaatactcc agcctctttt tgatttcgcg
    41281 ttcaaaaccg cgttcggcgg gctggtaaaa ttcatgacgt ttcaaaccgt ctgggaaata
    41341 gctttggccg gaaaaacctt cgagggtgtc gtggtcatat tgataaccca aaccgtaacc
    41401 ttcgcttttc atcaacttgg tcggtgcgtt catcgcgtgt ttgggcggca tcaatgagcc
    41461 tgtttcctga gcggcacgtt tggcagtctt cagagccata tagctggcgt tggatttggg
    41521 ggcacatgcg agataagtgc aggcctgagc cattgccagc tccccttccg gcaagccgag
    41581 gcgctcataa gctgtccatg cattgtttgc aattacaagg gcttgcggat cggcgttaga
    41641 aatatcctcg ctggccatgc aggtcatacg gcgcagaatg gtcgccggat cttccccacc
    41701 aacaagcata cgggccatcc aatacagcgc tccatcaacg tccgaggcac gcagcgattt
    41761 atgaaaggcg ctcaagaggt tataatgcgc ttcgttacct ttatcgtaaa gtggggcgcg
    41821 gcgctgaacc aaagccaata aaccttcttc gtccagtatt tcaccttggg cgatgatttg
    41881 ttcggccatg gagagcgcat agcggccatc gccatcagcc atggttttaa gaagtgcgcg
    41941 cgcttcgggt gcaagaggta gcggcgcgtc tttttccgcc ttttgcaaca aagcttcaag
    42001 agcttcatca tccaagcgtt taagcacaaa aacctgcgca cggctgagca gcgcggcatt
    42061 gagttcaaaa gacgggtttt ctgtggtcgc gccaatcagg gtgatggtgc catcttccat
    42121 aacgggcaga aaggcatctt gctgcgattt attaaaacgg tggatttcgt ctacgaatag
    42181 caatgtgctc cgcccgtttt gttttcggat tttcgctgca tcgaaaactt tacgcaaatc
    42241 tccaacgcca gagaaaatcg ctgagagctg ctcaaaatgc agatcggtat gatgggctaa
    42301 aatccgcgcc agcgtggttt taccgcagcc cggcggcccc cagaaaatca gagacgacag
    42361 gtgcccaccg tccagcattt tacgcaaagg ggcacctttg ccaacgatat gatcttgccc
    42421 gacaacatct tctatgcaat gcgggcgcag tcgatcggcc agcggcctac gctcgtcgag
    42481 agcggcacct tcctgatcta ctgcgaataa atcccccatt ttttataccc cttacgcttg
    42541 accttgaccg tttaaatatg tgagatatct ttttatatca tcaggcgcga acatttaaag
    42601 aacaaactta tgtcagcaga acggttagac tatacgggtg aagttgcgaa agcgaccacg
    42661 ccgctttatg agcgactgaa aaagcagtac agtcctgagc gctggcaaac tcttgctccc
    42721 tatattcacg agatcaaccg gctaaaaaaa gaaaagaatg cggtcattct ggcgcataac
    42781 tatatgacgc cggatgtgtt ttacggggtt ggcgacgtga tgggcgattc cctgatgctg
    42841 gcgcaaaaag cggcgcagag cacggctgat attattatcc aatgcggtgt gcatttcatg
    42901 gctgagacct cgaagatcct gtgccctgat aaaaaagtca ttattccgga tatgaaagcc
    42961 ggatgctcac tggctgaaag catcaccggc gaggatgtgc gcaggctgaa agaacagcat
    43021 ccaggcattc cggtcgtatc gtatgtgaac acgagcgccg atgtaaaggc ggagagcgat
    43081 gtgtgctgca cgtcttcgaa tgcattgaag atcgtcaatg cgctgggcga acaagggcat
    43141 gacacagtga ttatgacgcc ggataagttt ttggcacaaa atgtggccgc cgagacggat
    43201 gtaaagatta tcatctggga cggaacctgc atggtgcatg agcgtttcac cgccgaagac
    43261 gtcaagaaaa tgcgcgccga tcacccgggg atttttgtgc tggcgcatcc tgaatgccca
    43321 ccagaagtga tggcggaggc cgattatgcg ggctccactg cgcagatgga tgattatatt
    43381 aaggagcacc aacctaaaat ggcagtgctg tatacggaat gttcgatgag tgacaatatc
    43441 gccgcggcca atccgggcgt tgagatgatc ggcacctgcc agatgtgtcc gcatatgaag
    43501 cgcattacct tgccgaaaat ccttaatgcg ctgcaagatg aaggcccgga agttgagatt
    43561 gatgcggaga tagccgagcg cgccaaggca ccgattgagc ggatgctgga gttgagttag
    43621 cacagtgtca ttgtgaggag gcacggccac gaagcaatcc ataatatttg tacttgtgac
    43681 cctggattgc ttcgcttacg ctcgcaatga cgaaaggtaa aaatatttat gactttatcc
    43741 cccatcatca tcgaagatat tgtcaaaaca gccctgaagg aagatttggg acatgggttt
    43801 gatattacgt caaacctgct gatcccggct ggacaatcgg cgcgcggggt tttgcgggcg
    43861 cggcaagatg ggcggctggc ggggctgatt gttgcgcttt ccactttctc tctcaccgat
    43921 attgattttg acatgacggt catgaccgag gacggggcgg atttgagcgc cggggacgtg
    43981 atcgccgaaa ttgaagggcc ggtgcgcagc cttcttacag ctgagcgggt cgcattgaat
    44041 tttatgagcc atatgagcgg cgtggcgacg ctgacggccc gctatgtcga aaaggtaaaa
    44101 gatacgggcg ttgagatttg tgatacgcgt aaaactttgc cggggctgcg cttgctgcag
    44161 aaatacgccg tggagatcgg tggtggggcg aaccaccgtt tcgggctgga tgatgccatt
    44221 ttgattaaag acaaccatat cgcggcggtt ggcagcattg atgaggcgct aaatcaggct
    44281 catatgctgg ccgggcatac caagaaaata gagattgagg ttgatactct ggcgcagctt
    44341 gaagatgttt tggctaacgg caaagccgat attgtgatgc ttgataattt tgggctgaaa
    44401 gatctcaaaa aagccgtgca gctttgcaag aagcaaatta ccagcgaggc ctctgggagt
    44461 gtaaaccttg aaactgtacg cacaatcgcc gaaaccgggg ttgattatat ctcgattggc
    44521 gctttgaccc atagcgcccc ggcgctggat atcgcgctgg atatagatgt gtaagactat
    44581 ccttcaggtt aaactcaccc ctaattctac gaaaaatgct gttttgggct gggaagacgg
    44641 agcagatggc gaaaaggtcc ttaaatgctg cgtcacggct gcacctgaga agggtaaagc
    44701 gaacaacgct ttaatctctt tactggctac acattatggc attcccaaga gacgactttc
    44761 acttttacat ggagaaaaat cgcgtataaa aattattgaa atacaagaca ttgaaggtat
    44821 ttctaatata gccttcaaaa gatgataaat tttagctttc ccttcacttt ttaatggtat
    44881 aattatatga caactatctt tgggacgggc aggcaaattt aggcttatga ctttaagagt
    44941 tttcaaaaac catgctttga taacgggatt gggtgcgttt atgttttttg cgcatggctc
    45001 ggcgtgccgg gcggctggaa acatagaaac gctcacaaag cagaatgtta aagaatttat
    45061 tgaagatact acggatatta ccacgacgaa tacgcaagcg ctgtctcctg aaaaaattca
    45121 gcaatatctt gaaacacatc ttgataaaaa ggcgcgtttt aaaagcgtta tgaaatacaa
    45181 tataccgggc atgccgccgc aagaggccgc cttaagcctt gataaagaag agtttatgaa
    45241 atctgttgtt caaggtgccg aggccgttga tggctatgaa acacttgttg aaatcacgga
    45301 tattaagatt tcttcagatg gcgaaaaagc ctttgtgaaa acccaaaata ctgaatatgc
    45361 caccatgcct gtagcgacag aaaccggtag tgttgagaat gtgcctatgg aaggtgtttc
    45421 tgaatgtacg caaatcctgc tgctcaaaaa aggtgtcatt atgatgtata atgctaattg
    45481 cgtgaccaat attgagttta tggaatatta aaagctagcg acgcatcacc atcagttttt
    45541 tgatctcggc gatggctttg gccgggttga ggcctttagg gcatgtgttg gtgcaattca
    45601 taattgtatg acagcgatag agacgaaacg ggtcttcgag attatcaaga cgatcgccgg
    45661 tggcttcatc gcgcgtatcg gcgatccagc gataggcttg caggaggatc gccgggccca
    45721 tgaaacggtc gccattccac caatagctgg ggcagctggt cgaacagcaa aagcacagga
    45781 tgcagccggc gggacctcgg ccatcatcac cgttgagaat attggcatct tcggggcttt
    45841 gcagacgctc gcgccctggg gcggggctgt cggccttcag ccaaggctca attgaggtat
    45901 attgcgcata aacgtggttg aggtcgggca caaggtcctt aaccacaggc atatgcggca
    45961 gcggcgtgac cttgatatcg ccggagcaat cgtcaatcgc cttggtgcat gccagtgtat
    46021 tcgtgccgtc gatattcatc gcgcaggacc cgcaaatccc ctcgcggcaa gagcggcgca
    46081 aggtcaggcc agagtcgata ttatttttga tgtaaagcag cgcgtcgagc accatcggac
    46141 cgcatttatc gagatcgatg gtgaaggaat cgagacgcgg attggcttca tcatccgggt
    46201 tccagcgata gattttgaag gttttcttgc gtttggcatc tccggccaca tcgtgggttt
    46261 gaccggcagt gatttttgaa tttgcgggaa gtgtgaattg ggccatctgt gcgctcttta
    46321 tgcgttggtt ctatctgcca atcagaataa gcgcttaagg gcaaaatgtg aaggggcaat
    46381 ccttcacatg ttagaaggag acagtgctgt tgaaattatt cgggagcatc aggtttcacg
    46441 tctaatgttt gccgtctctc aaatactgac aaatcgtaaa tctccatacg cccgcgcgtt
    46501 gtttgcttta ctctgtagag cgctttgtca gcggcatcta ttatattttc aggcgttata
    46561 gccggtccag atattccgat ccctatgctt gagccaattt tatattcttc gcctttgtgc
    46621 tcaattgtga atgcgcacat ttcaccagcg agcctggtaa acgccgggtc aacgtcattg
    46681 ccattattca cttccatgat gacggccatt tcctcgccgc cataacgagc gacaatatcg
    46741 tctgcacgga cagactcctg cagatgctct gcggcttttc ttaagaccgc gtccccaacc
    46801 tgatgaccgt atgtgtcgtt aaatttttta aaatgatcga tatcgaccat aatcaacgct
    46861 atgcgtccac cggaccgcct cagtttcgtc agcgttttag gcagggcatc atcgaaatag
    46921 cggcgattat aaagcccggt taaagagtct ctaatagagg tatttcgatc aactgatgct
    46981 cgctgatttg cctgttccaa ctgttggatg agtgtctttt caccttgagc tttgtttcca
    47041 tcttcttgg
//
LOCUS       DAOSWR010000002        45461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_510_length_45461_cov_6.491631, whole genome shotgun sequence.
ACCESSION   DAOSWR010000002 DAOSWR010000000
VERSION     DAOSWR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 45461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45461
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_510_length_45461_cov_6.491631"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1251)
                     /locus_tag="PK513_00250"
     CDS             complement(70..1251)
                     /locus_tag="PK513_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80918.1"
                     /translation="MVRISSDNKKAKKILQNIAETLTANGAYIDPDIEIRCQNNSISA
                     HTDQNLKIRLAFQIPHELLLPYDLFEIGIKKDQFILKSTASKASPVQTKLIKAMLDLY
                     NATDQLTTHRTACPWLFYANQPAIIKMLLKAREGDDTNALRKLLKDPDQNRDHLELLT
                     FFKTRLLRCNLHKKWRKPSATLLPLVDSLNHHILGTHYNHIYTPNKGMLSLTARCPKG
                     DGQCMASYGRLDSLDSYLHYAFIDKDAPFVRSIPATINLDNEITIEIHGLNVGINQED
                     LPEQLKGLDFYIPAIEIHHEARRIIFSHLFIPQDAAKLSMRRILGYSLKTLDSKINEE
                     KYKQTLLQTEHKIVTTNNAYYQKLLNLYESVEHNQKNTDLKKLAQIQLKKIKNYMNFT
                     S"
     gene            1375..3768
                     /locus_tag="PK513_00255"
     CDS             1375..3768
                     /locus_tag="PK513_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO80919.1"
                     /translation="MKVPNMEQKAISLEEASTLAVQHMKRKNYRVAEMVLQDILSSVP
                     GHGDSLYLLGLARYYMGNLDGAIEQIEKAVEDEEAYAEWWCNYGIMLTECARYDEAIK
                     AFDKAIATDADYCESYWNQSHAYWLAGEYEKAEKTARKGVDLKPDSAEAWLNMGTAVV
                     RLGRLEEAVECWEKALEIQPDFVFAWNNLGNVLRELGRLEEAADKCRKALEIQPDYPQ
                     AQNNLANALMDLGDLQESEQLYRQAVTGKPDYAQAHNNLAINLIKQCRFAEAIQQARV
                     ALSYQKDYFEALLSLSTACRAVGQLEEAEKAVHRATLINPESAEVRIDLADLLYMQDR
                     YGDAEIELERARRLAPDTPRLYLKLADVLQRGNKVEEALQAVDKAAQLNPEMPEAFLK
                     KGMICHISNRIEEAKENFDKTLEMNPKAAGAFLAMAELSLSLGEKEQAYDYVCRAQEL
                     APDLPALFLTISKTKKFTEDDEDFQKMVKLEQSARNFGIDQASALNFALFSAYEDIGD
                     GDKAFEHLQKGNQLKRQLIPYDPAQQEASFKALQEVYSSENLKAMEGKGFETELPVFI
                     VGMPRSGTTLTEQIISSHADVFGAGELMEIGTLDMEFGFLSAENALRQGQRYIEQVKA
                     RDPSGKAKRITDKMPGNFSHLGKIVSILPQAKIIHTRRNPIDTCLSCYKQNFAMGQYW
                     SYDLEELARYYNNYLELMAYWREVLPERFIEIDYEETVSEFEPQARALIDYIGLPWDD
                     ACLAPHKQKRAVLTASKQQVTKPIYTSSVKSWKKYEKQLAPLIEGLASGPAKDLLDL"
     gene            3838..4326
                     /locus_tag="PK513_00260"
     CDS             3838..4326
                     /locus_tag="PK513_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOO80920.1"
                     /translation="MLADYKHKNPSKYLLVKLSLATVLSVFPGEVSAQSLNCFESLIF
                     GEFVTCGAAGSVTVRPDNSTTSSCVTVGSAPQTRGRCVVTQSFPFRPIQVNVSGTTTI
                     NSGGNSMSVTNVNIVTNNGGTGTTVTAPFLNVPIGATLNVGATQASGTYTGALGVTAI
                     LQ"
     gene            4364..5125
                     /locus_tag="PK513_00265"
     CDS             4364..5125
                     /locus_tag="PK513_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80921.1"
                     /translation="MAVSKSILTVSALAFALMFGGGVYVLSQLNVLAKPLVEKIASDA
                     LGADVSIGAMDISLPEKRVRVRDIHIANPEGFSKPHALGVGNIEVGLRSVSKGLIDFE
                     NIDVGAVQVFLEVQPDGTNLHKLKSGIKGGKEEAGKEPLKVIIRHFVLEGAQVYPSVT
                     LLDVQDLKPVKVAPVRLNGIGVKENGILAREAVAQVMAPLLETFSQAAGDAGFYQGMS
                     PGALKEMGMDQLEVIKEQIRQEVDNIGEGLKKIFD"
     gene            5623..7314
                     /locus_tag="PK513_00270"
     CDS             5623..7314
                     /locus_tag="PK513_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOO80922.1"
                     /translation="MHKILILTALFFIPFTNVAFADEIPVPSTLKAATVFADRATLTR
                     SVKIDLPAGKHVVVFENLPANLFPDSLRVEGKGTAKATLGALSHKIVNSVELTSEREQ
                     ELTANLETLENQKKIIAAEKQALSQKRQFLESLAARAVEKEDEAIAEFNLNTESWLAG
                     AETIYTGISEILRADLEKNKQIEDLQKQINKIVQDLNQRRTGQKQTTQVRLPVEATGA
                     GTLTLALDYQIGGVSWHPVYDARLDTQNGALELIQYGSVSQRTGEDWSNIELTLSTAQ
                     PHRGATAPNLPPMWVSLYDPTIARRQSVGDFGYGMAKSVVPADVMETEERTEMDASAP
                     MPVEAMKETPMRTATIDTGGFTAEYKIPGLSSIPADGTQTKVLIAPFETETKLEVHIK
                     PQANTNAYLIAKTTLKGEAPILPGPAFLFRDGAFVGQTGLPLLRPGGEHDLSFGIDDQ
                     IEVAHKTLKDERDEEGIIMNKTKSVERHTVTEIHNLRSKSVNIVLMQMLPSAKDKNIK
                     LNILTEKTTPGLETDHDKIKGLSVWDFALDPGAKKDVKLGWKLSWPQDKTLTGLR"
     gene            7384..9777
                     /locus_tag="PK513_00275"
     CDS             7384..9777
                     /locus_tag="PK513_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HOO80923.1"
                     /translation="MVNKTTSKQTLSLTNRMLAAFGRGSLITEKARLEAFLKAYPGEY
                     CGWSSDGSVVYSQSFCDVLGIKSIDKLIDIQGHLAPSDAAALEGLFERLEKNAIPFTT
                     HAKSHDKTKIFKITGSQGQDTENKDRFNILWLEDVTESFKTRETFEHDAQIKAHQLEQ
                     LQAAFHALPQPRWLRDKNGEIIWINQAYSDAAGLTISTILNEQKELPASTRKLKDKKD
                     ASLPPGPELAACALSSGEKQETQAHINIGGKRLLMKIIEMPLPELNMTLGLCEDISRI
                     EELETALKRYQASNIELLEQLRTAIAIYSNDARIEFYNSAFSQLWGLEDGWLNTKPKL
                     GEIMEKLRETRRLPEQADFRKFKQSWLDMFTSLIGPHDEMLYLPDGSALRMLVVPHSM
                     GGIMMTFEDVTSRLELESSYNTLIAVQKETLDNLGEAVAVYGGDGRLKLWNPAFGRLW
                     NLNPEDLDGKPHVSKIVEKLSGFFTAEEWPRRKEELTAKALDRTMHEGRLRRANETLI
                     DFTTVPLPDGGVLITYIDVTDTVRVENALREKNAALEAAEQLKLDFLANVSYQLRTPL
                     NAIMGFNEILDQEYFGKLNDKQKEYTRDTRKASEQLLDLINDILDLATLEAGYMTLQR
                     SDIGIYDFISNIQKRVSDWARKAEIEVHIKCAKNIGKLSADEKRLKQAIINVIRNAIN
                     FTPAHGEITLCAKRKKEGIEFTVQDTGIGIRKEDKLRIFEPFERAGIGEANSKKGHSG
                     AGLGLSLVKNIVALHGGNVNLESAQGKGTCVTLFIPFTSIETPLKLPLDKVKKAAKN"
     gene            complement(9793..10221)
                     /locus_tag="PK513_00280"
     CDS             complement(9793..10221)
                     /locus_tag="PK513_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80924.1"
                     /translation="MPQIAAAQNENLDIVQEPVCFAVRNAADYKVYGTIATEQFMRPD
                     GIIARHRSNFRLDEAGGIDEKTGEASDRVEFCSYGPFFLNRMLIVTLRTLFPVFECKT
                     RVDTGKEIVIRGARRADDSGVITWAECFQADGTPTGKPPE"
     gene            10364..10792
                     /gene="tsaE"
                     /locus_tag="PK513_00285"
     CDS             10364..10792
                     /gene="tsaE"
                     /locus_tag="PK513_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOO80925.1"
                     /translation="MKHISDNEKDTSRIAAALAEDLQPGNVLLLHGTLGMGKTVFARA
                     LIRALTNQHDIDVPSPTFTLVQLYDGLTCPIYHYDLYRIEHPDEIFELGWDDALADGI
                     TIVEWPDRLGPYKPPLSLDITLSNADNDPNKREILIEKNA"
     gene            10789..11523
                     /locus_tag="PK513_00290"
     CDS             10789..11523
                     /locus_tag="PK513_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO80926.1"
                     /translation="MTTAAKSMSDRAFILAAGFGKRLRPYTNDIPKPMVEVGNGAMID
                     QALDKLGAIGIRSCVINTHYKAEILQRHLQTRRRPEVILSHEPDLLDTGGGIKAAISH
                     FDAPFLVLSGDSVWEDAPGQNTLETLAAYWDPARMDILILLQAIETMTLTHALGDYDL
                     DEDGHAHRSINKTGRYMWTSIRINAPEIFGNAPDGPFSYLDLLDTAQAKGRLYGLVHH
                     GEWHHISTPADLDAVNAALKTRSGQS"
     gene            11520..14531
                     /gene="addB"
                     /locus_tag="PK513_00295"
     CDS             11520..14531
                     /gene="addB"
                     /locus_tag="PK513_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HOO80927.1"
                     /translation="MNALNAPAKNITTISAGQPFSRLLADRLLEDYAGREESLSQTLI
                     LLPTRRACRVLQDSFLALREAKPLILPRMQPLGDLDEEELSLSITGQTGQDHAWSLPA
                     ALPDLQRQILLAKLIRQIHTDDSLYSASHEQTLKLAQALGMFMDHVYTENLDMADLAN
                     LVPEDFAEHWQITLKFLEILSEHWPKILAEKGVIDQADRRNRLILALAKHWQACPPKT
                     PVIGAGSTGSIPATARLLSVIAELPEGEVILPGFDLDLDEDSWNALGETHPQFGFKQL
                     LGRMKIDRETVKPFKNVQNNPHNNNRQILARQLMRPAETAQRWAEIAECAQSSAALKN
                     ALDDLSLISCQHLREEADVIALLMRQTLETPDKTACLITPDRELAARVAASCQRWNIT
                     IDDSAGQNLRHLPIGIFILAVLEAISEDFSPIALLSLLKQGFCQMGKNVQAHVCQTTA
                     LDFVLRGNKPSAGFTGLKKHIEKQESLDENLKAEVLGLLSELEDIFTPLLALQAEPKT
                     FRQILQTHLHICESLCASEDREGAQNLWHGEIGRHASRFFSELFDHAHALDTLNLRDY
                     AAVLAHFMNQTQIRPAFGTHPRLQILGTLEARLIDADLVILGGLNEGVWPPEPATDPW
                     MSRPMRKEFGLPAPERGIGLAAHDFVQGLCAPCVILTRAEKQDGAPTVPSRWLQRLEA
                     VLHAANLETPQGGNILHWARLLDHTSEVKPIDMPSPCPPVEMRPRHLSVTKIETWLRD
                     PYAIYARYILGLKILDPLEKPVDVAQRGQLLHKILERYVLETRDALPDGSAALALKIA
                     REEIDRRGDDPHLWSFWWPRFAKTAQWLDSHERGLREATRNLAVEARGEIDIKAPGGD
                     FNLTAIADRIDLLPDGKAALIDYKSGGTYSAKAIENGTLPQLPLEALILEKGGFKNLK
                     PYVTGSLQYWVLNGTGDGAKLTIYDNALDELVKRTAENLTALITSYDNPDRAYLCQPR
                     PDQAPRFNDYEHFERVKEWAISADSEETEAA"
     gene            14528..18058
                     /gene="addA"
                     /locus_tag="PK513_00300"
     CDS             14528..18058
                     /gene="addA"
                     /locus_tag="PK513_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HOO80928.1"
                     /translation="MSAQVAPRKQTETVREVEPNVLQRRASRPDASVWVSASAGTGKT
                     KVLTDRVLRLLLPREERPGQIIPGAPAHKILCLTFTKAAASEMALRISKTLSAWAIMP
                     LDGPEGTKTLYAELRGLLGRAPSTIEIKAARKLFADTIDVPGGLKIMTIHAFCQSILG
                     RFPLEAGINPHLTILEDSASEALLTAARNRAFANAEKQPGSELGQALERIAATINEDQ
                     FLSLLNDITRERRQLARLKARYFDLEGLYSALCVHLGIAPHTTPQNLLNDACSDESFN
                     ANALRSAALTMQKGKGQDQTNAEIIFNWLAASTEQRMQDFSLYQSAFLKKSDGQIKAR
                     LALKNSIEANPEILDILTREGERLIALKDAMAASSSAALTRDILILGESILDEYEILK
                     TGQGALDFDDLILKTLDLLRGKSMGLNAVNAGSWVHYKLDQGLDHILIDEAQDTNPEQ
                     WQIIEALCDEFYSNAPDNNMTRTVFTVGDEKQSIYSFQRASPEEFARMQSDFERKTKN
                     ALQTWDSVPMNISFRSTSSVLNAVDSVFAAAHTRKGLSALPIEHTAFRRGQAGLVELW
                     PLFESDECGQTDLWTPLTSAEEAQTGRKKLSVHIAKTIKTWLSTQEILPSKACPIRPG
                     DIMILVRTRSNLVNQIAYELKNLNVPVSGLDRMVLSQELAVQDLLAAAECALQPLDDL
                     TLASVLKSPLIGLNEDEIFNLSAYRGKKSLWESLQASDHQKITAYLKNLLTLSKKTTP
                     FEFFSHILQSPCPTDTISARRAIMRRLGKDTLDPLDELLSLARTVEKQSPPSLQKFIH
                     DRRASHQEIKREHHSDNGETDGEVRIMTIHGSKGLQAPIVILPDTITSAAKEAARAEK
                     RLLWPNQTGLNLPLWSPRKECDYQAYREAMGALNQRLDEEYRRLLYVAMTRAEDRLYI
                     GGALGKSQSPEKLPGGCWYDLIAQGLQNLEDIEDLKNGGLRLTNPQIHEPEKPKVPGK
                     TAPLIEPTPQWLHTHPPSESQTTTIIRPSHIADTAISPLQGTNNQRFVRGNLTHKLLQ
                     LLPTIPRKNWENTAQAFLKRYAKTLSAEIRGEIACETLKVLNHPDFAPLFGPGSMAEV
                     PISGHLEGKGLISGQIDRLYIDENTISIIDYKTNRPPPENPAQIPEIYRSQMHTYAAV
                     LKKIYPGRTIHAALLWTDGPILMPLDLKIM"
     gene            18109..18429
                     /gene="trxA"
                     /locus_tag="PK513_00305"
     CDS             18109..18429
                     /gene="trxA"
                     /locus_tag="PK513_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOO80929.1"
                     /translation="MPSVAVTDSNFDSEVLQSDKPVIVDFWAEWCGPCKQLSPVIDEL
                     AEDLGGAVKVVKVNIDEAPEAPTKYGVRGVPTLMIFKEGQVVDTRVGGMPKSQLQEWI
                     EGQV"
     gene            complement(18471..19007)
                     /gene="infC"
                     /locus_tag="PK513_00310"
     CDS             complement(18471..19007)
                     /gene="infC"
                     /locus_tag="PK513_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023003529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOO80930.1"
                     /translation="MNNRPPRKDDGPRINDDITASEVRVVDADGEMQGVMSVAEAIKL
                     AEEAGLDLVEVSPNASPPVCKILDFGKYKYEQQKKASEARKKQKIVDVKEVKIRPGIE
                     EHDYNVKMRSARKFLKNGDKVKVTMRFRGREMAHQDIGFDLLKRMTEELADVGKVDLQ
                     PKMEGRQIIMVLSSEVQK"
     gene            complement(19141..19602)
                     /locus_tag="PK513_00315"
     CDS             complement(19141..19602)
                     /locus_tag="PK513_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOO80931.1"
                     /translation="MKYIAPTLWILVFQVIGWAIGLLTRQNMGWYDTLIKSPFNPPDI
                     VFPIVWSTLYALLALAGWLVWNARKKPGGNQAHNLYWMQMFLNWGWTFIFFEFHLILL
                     GFFWILAYLVAMLAFVAAAWTVERRASLLVIPTIVWGCFAAYLNYSIWTLN"
     gene            complement(19609..21612)
                     /gene="thrS"
                     /locus_tag="PK513_00320"
     CDS             complement(19609..21612)
                     /gene="thrS"
                     /locus_tag="PK513_00320"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO80932.1"
                     /translation="MEQKAEKQIQITLPDGAVRTYASGVTALDIAEDISKSLAKAVIA
                     VQIDGELSDLSLPITGDASIALVKRNGDDDKDIALEMIRHDAAHVMAQAVQSLFPGTQ
                     VTIGPAIENGFYYDFARNEPFSTEDLEAIEKEMRKIVERGAPFEREVWDRGAAIAYFK
                     DKGEYYKAELIEDLPEGEEIKIYRQGQGENGWLDLCRGPHMPTTRHVGTAFKLLKVAG
                     AYWRGDSDKAMLTRIYGTAWRDDKELKAYLTQLEEAEKRDHRKLGNEMDLFHFQPEAQ
                     GSCFWHPKGYVIYHALEDYIRRRVNDDGYNEIKTPQLIDNKLWEKSGHWGKYRENMFV
                     VPDEIPSGEDDGPVVSDKAFEKLMALKPMNCPAHVQVFNQGIKSYRDLPIRLAEFGCC
                     HRNEAHGALHGLMRVRQMTQDDAHIFCREDQITEESIRFCNLIKRVYTDLGFEDVRVV
                     LALRPEMRAGSDEVWDKAENGLRKALNEAGLEFEEEEGEGAFYGPKVEYHIRDAIGRS
                     WQCGTLQLDFVLPERLDASYIGEDGAKHRPVMLHRAVLGTLERFIGILIESTGGKLPL
                     WLAPVQCVVATITGSADDYAKDVCAALKAAGIRADLDIRNEKINYKVREHSVAKVPNL
                     FVVGAREAEERTVAIRRLGSNDQNVDPLDDAISALVQEAQPPF"
     gene            complement(21625..23040)
                     /locus_tag="PK513_00325"
     CDS             complement(21625..23040)
                     /locus_tag="PK513_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOO80933.1"
                     /translation="MASPSGQGRPIYDDKKTINDTDEWQGVRDSWAKEQAGKVHARAL
                     AVFDTDDDMPLSKHLLFMAITSFFVIFIVWANFASLDEVTRGNGKVIPSSDIQALQSL
                     DAGVVEKILVHEGDLVEAGQALMQLDQIEAESDLGANKARYLGLLASITRLSAEAEGK
                     GTVEFPQEVMKGAPQSVTEELNAFRANQQSMNGQRNVLQQQMSQRESELREVGTRIAD
                     TRGVIALQRQEAGMVRPLVEKGSAPKMELLQLERTIKEKNAELNSYTSSVPRIKASIE
                     EARGRIAELETNMKAQAQAELSAKLIEMNEIKERLAALKQRKGRTVLKSPVNGTIQEI
                     TVSTIGGVVRPGEDIIKIVPKDDQLIVEAKVSPKDRAFIFPGQKAVIKLTAYDFSIYG
                     GLDGELVDISADTIEDQEGNSFYRVKLRTDKTELVRKGEVLPITTGMVASVDILTGKK
                     TVMQYLLKPFIKTLDNAMNER"
     gene            complement(23124..25439)
                     /locus_tag="PK513_00330"
     CDS             complement(23124..25439)
                     /locus_tag="PK513_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOO80934.1"
                     /translation="MVNDKSSKESKDVQEPQDAPDNGVAVNIVDKEGDPWPLQADRMA
                     IRMPLVDCLRIMAGHYGRRTSNDALVAGLPIPNKGITPSLFIRAAERAGMQAHLADRS
                     LDSLAIAPNLPCILALEEGQACILWDIKYPEKHTPKKRDNQNVVIHPETAFIVQFPET
                     PDERQKLTLEQLKNLYSGYAFFIRPVARVDDRAGPAEIDTGRDWFWSAFWKNKTLYKE
                     VAVAAIMINMFGIAGSLFIMNVYDRVVPNNAFETLWVLAAGILIVYFFDFMLRNLRAH
                     FLDYAGRKADVVISSALFEQMMGMSMEARPASAGVLAANMKEFETLRDFFTSATMVAL
                     IDLPFVLLFIAIIAVLGGPIAFVPLAAIPLIVGMGFFLQKPLQKVIKASLQESALKNA
                     LLFETIIGLETIKVQAAEGHTQRSWEELTDKASRTSVKSRQIAAFAQNWAMFVQQAVS
                     AVIVIVGVYLIAAGSLSMGGLIACVILSGRAMGPLAQVAGLMTRFSQSHEALKQLDDL
                     MKKPVERPAGKHFVTLPHIRGQLEFRDVVFHYPGQSTPALNHISLTIEPGEKVGIIGA
                     VGSGKTTLERLLINLYAPASGSVQIDGTDVRQIDPGDLRRNVGSVQQSPQLFFGSVRQ
                     NITMGHETAPDRAVLRAAQLSGVMEFLKDSQAGLDTQVGERGEALSGGQRQAVAIARS
                     LLYDPPILVLDEPTASMDPASENRLKKRLDEICRDKTVMLITHKGAMLDLVDKLILID
                     RGRLVAYGPKDEVIRKLQARQYGTEAENTQV"
     gene            complement(25510..26925)
                     /locus_tag="PK513_00335"
     CDS             complement(25510..26925)
                     /locus_tag="PK513_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOO80935.1"
                     /translation="MILKKNSIRMLCLTVSSVALLGLSAGSALSQDEALHQSLSVSLK
                     DAVATGVSTNPEYGVVAASRRATDEELKQGRALYFPSIDMRADAGFEHSDDPATRGGT
                     DSDDEENLLRKEVSLTLTQMLFDGFDTKYEVERQKARVNSSANRVRETAELVGLSIVE
                     SYLEVLRQRQLLVISRQNVADHISILDQIQDGVDAGRSTQADMEQAKARLAAAKATES
                     STRQALRNAESQYRSDVGDAPGQLSMPVIPYDVLSASAEQEVEKVLAYSPTLDIFESD
                     IEVAYAEAHQTRSTFYPQVDFQLNGRTGHDINGVEGTDTSASALVVVNWNLYRGGADM
                     ARSREFLHRHQQSKESRAEAARQVENDVRQTWAAMVSAGERAREFAAQADANVEVVKA
                     YKDQFNLDRRTLLDVLDAQNELFVSRSNTINAEFLEMFAVYRLMALKGSLLPTLGVEY
                     PRESVVAADDSWSTSKKMEAR"
     gene            complement(27197..33967)
                     /locus_tag="PK513_00340"
     CDS             complement(27197..33967)
                     /locus_tag="PK513_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5801 repeats-in-toxin domain-containing
                     protein"
                     /protein_id="HOO80936.1"
                     /translation="MANENADASQPSETVTNTEENQVNVTLVDGVCVVTTEDATETLT
                     APIIIEKPAAGKTTQIDVAAGQKYFFDFDKNNVQSFEQDGDNLTLRFADGSAVILNGF
                     GVAASSVLPATLTFGNTLSADELKRLIKVVDTTPGEEELQEPQAEIREVAKEASSTEK
                     TTEGEGKDTQVASVEPASGEQSAQQLAQIEPAAGEQPGGGIQNTGYGFNSSPEITPVG
                     PLAAVGPIDPTALQYGIEFQNDTLFLEEEPDDQPEVAGPQKHILDETNLNLSQSGKID
                     AEFGNDGPGVIIPNGAFAVSGSLLGGVLSSGGQAITITPTANGYVGTINGGATTVFEL
                     VINPATGEYIYNQLLPFDHADATNPNDAVTLDFGVIAKDSDGDTATTKIIITVLDDAP
                     VMADPDDESINENALSGGPVTVGDTLNIDYGNDTPGEMVQNGTSSATGALLGGVLSSG
                     GQAITITPTANGYVGTINGGATTVFKLVITNATTGTYEFTQFAPLDHSAAGTNIDLNF
                     GVNVVDYDGDALDTTITIHITDSVPDINDKPEVGKGIETVDETNLPGVSVSGKLDIDF
                     GTDAPGVLAPNGSTNSSVPLTSHGQPVTITQTTTGYVGSTAAGDVFTLTIQPDGSYTF
                     NLLGVLDHPDATNPDDAIQLVFGVTASDSDGDTDTGTITINVKDDGPIAYDDCNEFSL
                     QAINKDFNVVLVLDISGSMAGERLTLLKAAVANLLGDFNDYKGGEIKVHIVPFSTDAG
                     AGQTFDVSTNTGFNAAVNYLNGLNADGLTNYEAPLASAIDWLQGASANDPIPGADTYT
                     YFVSDGAPNRYLDDLGNVVNPPGTDAEEQAILQAEITGTSVTTSDGTFGDNSDEVGTL
                     QSLSKEVIGVGIGVSGTTLARLGVIDSDGNALDVQDPNDLDAALQGTNPLKGSATGNV
                     ITGENGGPNATDDLSEDVQNTVTKIAFKGTVVVVDPVNGATIDGDHGTLKIFADGSYT
                     YTVISGSAKNDIVEQFVYTLTDYDDDSREATLTLKGNVPLSIDADELLVDESDLNPTD
                     TDSGVVTGNFAPGVTVDYDSDGGFSSSVPLTSQGQPVTVTVVGNDYVGKVGTEIIFTL
                     ILNQTTGAYTFTLIGVLDHPDATNPNDPITLSFGVTANASDGDSDSGFIQVNVLDDGP
                     VAVNDGNTVLQNTTTVNGNVLANDDGGEDVVATVTNVRFNGVDHAVVNGIPTVVNGQY
                     GVLTINANGSYTYVSNGTNPNAVQESFTYTLTDFDGDSDTANLSISIDDVDTRPEVGP
                     TEVSVDETDLNPTDSASNVVDGNFGSDGPGTYVVTGAGTFGFIGAENNQLTSGGIPVA
                     ISVVGNSYVGKAGADTIFTLVLNETTGAYTFTLIGTLDHADETNPDDVIKLTFGVTAE
                     DADGDTDTGFITVNVYDDGPVAVDDTGTVAQNTTTVNGNVLANDDGGEDVVATVTNVR
                     FNGVDHAVVNGIPTVVNGQYGVLTINANGSYTYVSNGTNPNAVQESFTYTLTDFDGDS
                     DTANLSISIDDVDTRPEVGPTEVSVDETDLNPTDEASNVVDGNFGNDGPGTFTVTGAG
                     TFGFMGAENNQLTSGGVPVTVSVVGNSYVGKAGADTIFTLVLNETTGAYTFTLIGTLD
                     HADETNPDDVIKLTFGVTAEDSDGDTDTGTITVNVKDDGPVAVDDGALVDQGQTTING
                     NVLANDDSGADVPASVISVSFGGADVPVVAGTPSVINGQYGTLSINADGSYSYVSNNT
                     NTTQVQDVFGYTMADYDGDPDSALLTITVADVPELFIVGNNVDDIDGQTTPWVIGSGS
                     EAIIGGAGADVLVGDYGGSQVVNQTQDYNFVYILDTSGSMGTNNPADGVTSRVEIMLE
                     AVKNQMAQFDAYQNGQIKVHLVSFSTDVKSTFTVDFASTTALADVTAWLDAQTGTGLT
                     NYESPLQAANAWLSGTEPLGGNAITTTYFISDGEPNRYVNDQGTVLNPPGNAAEEDAI
                     IRAEITGTTVTTSDGTFGDDSNEVGALKALSDDVVAVGIDVPDNQNLNLIDSDASATY
                     IDDANDLQAVLAGNNPLNLLGSVGNDDIQGGNRYDLIFGDTLNTDDLADTQGLATNDG
                     AGFEVFERLENGEGSDTGWTRADTINYIRANAESLAVESVNTQGQGRQGGDDTITGGA
                     GDDVIFGQEGNDRITGGEGSDTLYGGSGQDDFIFEAITDGVDTIKDFNVAEGDVLDVS
                     ALLNGYDALQDSINDFVFATEVAGNTVIYVDANGSGNIANATQIAVLEAVTGLDLTLA
                     TNNGETTV"
     gene            35215..35715
                     /locus_tag="PK513_00345"
     CDS             35215..35715
                     /locus_tag="PK513_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO80937.1"
                     /translation="MAFQFEKLSVLIVEDTIPMLKLVSSVLDTLGVGKIYTASNGDEG
                     YKIYCEHNPDIVVTDWHMSPISGIELVDKMRNNSSSPNRMIPIVMMTGFSAMPRVAQA
                     RDTGVTEFLVKPFSANDLARRIAYVINRPRDFIETPDYFGPDRRRRKGENYKGPYRRA
                     SDLLVP"
     gene            35832..36335
                     /locus_tag="PK513_00350"
     CDS             35832..36335
                     /locus_tag="PK513_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80938.1"
                     /translation="MSKTNNSENKPRIYKADTHLQNKVGTGPLDEKAIERCQNVMNNN
                     DVDFAPLAMEFLEKLENAIKSAIARKKSKEESIQEMTNAVMQLKANASTFHYTLIGNM
                     ANIMLSFLEGIQEIDNDAIAIVNAHHQTLKAIVLKKMSGDGGAIGKQMEDELKAACKR
                     YFAKRMR"
     gene            complement(36395..36775)
                     /locus_tag="PK513_00355"
     CDS             complement(36395..36775)
                     /locus_tag="PK513_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80939.1"
                     /translation="MSKSETGAKSDAKPLQEEFSGAAAVVERLLQDLCVRMDEMLARQ
                     DTAFEEIKEQLGGMNRILDRTEQHIAALREGSGGRSCRTEVSEDFTNSAPYPGACFPK
                     PRCTLDRLGEIVRLSNGGPFLRIP"
     gene            complement(36851..38428)
                     /gene="serA"
                     /locus_tag="PK513_00360"
     CDS             complement(36851..38428)
                     /gene="serA"
                     /locus_tag="PK513_00360"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOO80940.1"
                     /translation="MAKVLISDKLDPLAVEIFEKNGIEVDFKPGLSPEEQLAIIDQYD
                     GLAVRSATTATPELIRAGKKLKVIGRAGIGVDNIDIPTATENGVVVMNTPFGNSITTA
                     EHAIAMMFALARQIPQANESTHAGKWEKKKFMGTELYNKTLGVIGCGNIGGIAATRGV
                     GLKMHVIAFDPYLTEERAADLGVTKVELDELLENADFITIHVPKNDKTANLINKDSIA
                     KMKDGVRIINCARGGIVNEADLKVALESGKVAGAALDVFEKEPATNNPLFGMDNVVCT
                     PHLGAATTEAQVNVALQVAEQMSDYLNTGAVMNAINMASISAEDAPKLKPYLKLAEQL
                     GSFAGQISNTAIKKIEIEYEGTITQVNTDPITSTLLASLLGQQTESVNMVNAMQVAHA
                     RGIELKQSYNDESKNWRSMINVIVTSEERTRNVTGTLFTGKEPRIVNIEGVPIEVALK
                     PHMIFIRNDDQPGMIGELGTLLGQEGLNIADFRLGRKEDASGAICLVALDGPVNDEVF
                     AKIKAQKQITSAKRLSF"
     gene            38594..39226
                     /locus_tag="PK513_00365"
     CDS             38594..39226
                     /locus_tag="PK513_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80941.1"
                     /translation="MLKGTDIKKGFLRARYKVATSLLNINEQHVVRLASAMAPPTEDI
                     GRWEETVAIHEAGHAVTAMALPHISVSYINILRETTAVNYGNSKQELGDAIISLLAGG
                     EAVKMSKRTRHMTTGDGQDLHLAEIYARTLLIVEGHNQSAPDFERSVRRMLATAKMKA
                     WEILMQHWPAVEAIAEVTRILPILDQRKLHHLFEEHRQQKLEGISADCSV"
     gene            complement(39236..40414)
                     /locus_tag="PK513_00370"
     CDS             complement(39236..40414)
                     /locus_tag="PK513_00370"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOO80942.1"
                     /translation="MMEKPALKPKNPCFSSGPCAKRPGWSFDNLSHALLGRSHRAGPL
                     KKQLKDVIEKQKELLGIPQDYVVGIVPGSDTGAFEMALWSLLGPRGIDVFAWESFSSG
                     WLSDITKQLKLENVNAYEAEYGQLPDLSQTDPANDCVFVWNGTTSGVRVPNGDWISAS
                     REGLTFCDATSAVFAYDLPWDKLDVTTWSWQKVLGGEAAHGMLVLSPRAVERLESYTP
                     AWPLPKVFRLTKKGALIGGIFQGETINTPSMLAVADALDALRWVENIGGLRTTIARAQ
                     ENLKAIENWVAKTDGFEFLAEDPASRSATSVCLKITDAWFQGLPEDEKTPFIKAMCKL
                     LEEEHVAYDIAGYRDAPAGLRIWCGATVSGDDVEALMPWIGWAFAAAKMEKQRQSEAA
                     "
     gene            40539..41441
                     /gene="serB"
                     /locus_tag="PK513_00375"
     CDS             40539..41441
                     /gene="serB"
                     /locus_tag="PK513_00375"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOO80943.1"
                     /translation="MSTVLTLVASDPRRPLCDGHTREVIKILGAYNVQPTCQPIWIKP
                     KKVAEIGVSDNTSSALFMHLRDFLEKDAIDVFITPIENRRKKLVLADMDSTIVRGETL
                     DDLAEHAGIKDQIAEITARAMNGELDFHAALNERVGLLKGLGIEALDKTLSTMIINPG
                     AENFIRTMHKHGAHCVLVSGGFTFFTSAVAKQVGFQAHHGNVLEIEDGALTGKVKPPI
                     LDKHAKVECLRTYMDRFNLKPKDCLTIGDGANDIPMLKTAGLGIGYKPKPAVANQIDN
                     LIIHGDLSAALYAQGFTDSDFYLT"
     gene            41571..42527
                     /locus_tag="PK513_00380"
     CDS             41571..42527
                     /locus_tag="PK513_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO80944.1"
                     /translation="MSEQWSYSVTNNELEELFRLYCTDFTTLAKQGRYGPITGRDKEV
                     DETILILMQKGRKNAVLLAPAGVGKTAMVVALAQKIAADDVPDYLLDARVIEIDMARM
                     AAGTNSPAEFQERFIPICKGVAERYHNPENPRIILFMDEIHQIMPGCVGSSYAGLSDV
                     MKPYLTAGDLMVIGATTLDEFRMYIAEDAALERRFQKVFLSVPNTAETYAILKALRPS
                     HEKHHGVTISNNNLMTIVQLTDEHMRKRNQPDKSIITMDSAMAYHVKTYGRNSEVTLD
                     SIYYMIGRETGLNKQALHDEKKIQRIMKQVREIEGEGYTYYI"
     gene            complement(42713..43750)
                     /locus_tag="PK513_00385"
     CDS             complement(42713..43750)
                     /locus_tag="PK513_00385"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO80945.1"
                     /translation="MALLDDAALQILFYPFESGALPWPSAGQKTLFLNAQVTLALRQF
                     DQCDLTLQQYFKPYESALQAQGYTALPGLPDEGTSYDVALLAAPKNQIETLYLMAYAL
                     EILKSGGLLVVAAGNKAGGGRLAKNMDTLGLYDICSESRHKARVVWAYKDNVDKAARK
                     KALAAGQPKEVIDGTFRSWPGIYGWNKVDQGSALLAQHLPNDLQGKGADFGCGYGYLA
                     RRVLAHCAVKSLACLDADWRALEMARQNLARPDISAALEFLWCDCTKHSLRGLDFIVM
                     NPPFHEGKSADITIGEDFIVNACHSLKPGGALWMVANKHLPYEAILKQHFKAFEKIFE
                     AQGFKVFCAQK"
     gene            complement(43796..>45461)
                     /locus_tag="PK513_00390"
     CDS             complement(43796..>45461)
                     /locus_tag="PK513_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOO80946.1"
                     /translation="GYNMATDGTDIIFFTGTLQQITVTLVNPYSSKSYDVDEEFRVNS
                     SAYDGLGGTDFLFMTDDGDFLSLTDNVGMQVVHNIEQFFASNGGDVINLADANTTYGD
                     VIILGGAGDDILWANIGNDQIFGSYGNDIIDGGPGNDILNGNDDNDQIFGGQGDDRLD
                     GGAGDDILYGGTDLGLMDLDKDFIDNITFPELIEGTNIQSLIPPGTPALGINSDNLTV
                     SYDASATLTFRDGFAGYNNTLGIYSITADGTIEMASVLWANVKDAGINIDHQIELPVG
                     EAGGQFGFFIIADGDRVNSEYLGLDITGDNVISFVYDYGGVNERAATINDDGNLVSVV
                     YNDGTTEQVLNGYHYHTTPRGDSPDINWDGDTHAVSGLLDLANQNVLRIGFEDLPNLG
                     DADYEDVLFDLDIDRIRVDASEQGSDTLIGGLGNDILYGEGGDDVLFIGEGFDQAYGG
                     TGSDTFVMLLPDALADKIFDFEAGVGGDILNIADILVGYSTVDDALSDFVQLVQNGSD
                     TELHINADGDIGGTFEAIAVFDGGLNATLADLIADGNIEAETSIPV"
BASE COUNT        11871 a        12277 c        10130 g        11183 t
ORIGIN      
        1 atagacgtac aacggtagtt tttttatcta tcctaaaggg ttatacgtct tctgtaagcc
       61 ttttccactc tagcttgtaa agttcatata atttttgatt ttttttaatt gaatttgtgc
      121 caattttttt aaatcggtat ttttttgatt gtgttctacg ctctcgtaca ggtttagcaa
      181 tttttggtag taggcgttgt ttgttgttac aatcttatgt tctgtttgaa gtaatgtttg
      241 tttgtatttc tcttcattta ttttcgaatc cagtgttttc aggctatagc ctaaaatacg
      301 ccgcatggat agttttgctg cgtcttgagg tataaagagg tggctgaaaa taatgcggcg
      361 ggcttcatgg tgtatttcga ttgccggtat gtagaagtcc aagcctttga gctgttctgg
      421 cagatcttcc tgattgatgc caacatttaa tccatgaatt tcaatggtta tttcgttatc
      481 taaatttatt gttgccggga tggatcggac aaagggcgcg tctttatcta taaaggcgta
      541 gtggagatag ctgtcaagag aatcgagacg gccatagctt gccatgcatt gtccatcgcc
      601 tttgggacaa cgcgcagtca gtgagagcat tcctttattt ggcgtgtaaa tatgattgta
      661 gtgtgtgccc aggatatgat ggttaagaga atcaactagc ggtagcaaag tggcgctggg
      721 ttttcgccat tttttatgaa ggttgcagcg taaaagtcgt gttttgaaaa aagtcagaag
      781 ttcaagatga tcacggtttt ggtcgggatc tttgaggagt ttgcgcagtg catttgtgtc
      841 atcaccctct cgtgctttaa gcaacatttt tattattgca ggctggtttg cgtaaaaaag
      901 ccagggacat gctgtgcggt gtgttgtgag ctggtctgtc gcattataaa gatccagcat
      961 cgcctttatg agcttggttt gaacgggact ggctttagat gcagttgact tgagtataaa
     1021 ctggtctttt tttataccaa tctcgaataa atcgtagggt agtaaaagtt cgtgagggat
     1081 ttggaaggct agacgaattt taaggttttg gtctgtgtgg gctgagatag agttgttttg
     1141 acagcgaatt tcaatgtcag gatctatata ggcaccattg gcggtgagcg tttctgcgat
     1201 gttttgcaga atttttttgg cttttttatt gtcggaacta atgcgaacca agatttagta
     1261 tatccttgtt acaaacttaa aaattatatg cgtatgcttt cttacgtgag attttcagct
     1321 atagtctttt tattctatag ttcaaatgtt aaagcgcaat atacacgtca atatatgaaa
     1381 gtcccaaaca tggaacaaaa agccatatca cttgaagaag ccagtacttt ggctgttcag
     1441 cacatgaaac gtaaaaatta ccgtgtggcg gaaatggtgt tgcaggatat tttatcctct
     1501 gtgccgggtc atggtgacag tctctatttg ctgggtcttg cgcggtatta tatgggtaat
     1561 ctggatggtg cgattgagca gattgaaaag gctgtggaag atgaagaggc gtatgcggag
     1621 tggtggtgta attatggcat tatgctgacg gagtgcgcgc gctatgatga ggcgatcaag
     1681 gcttttgata aagcaatcgc aacggatgcg gactactgcg aatcttattg gaaccagtcg
     1741 catgcttatt ggctggctgg tgagtatgaa aaggccgaga aaactgcgcg taaaggggtt
     1801 gatcttaaac ctgacagtgc cgaggcgtgg ctcaatatgg gtacggccgt tgtgcggctg
     1861 ggacgtttgg aagaagctgt tgagtgctgg gaaaaggctt tagagattca gcccgatttt
     1921 gtttttgcgt ggaataatct gggtaatgtt ttacgtgagt tggggcggct tgaagaagct
     1981 gcggacaaat gccgtaaagc gctggaaatt cagcctgatt atccgcaagc gcaaaataat
     2041 ctggcgaatg ctttgatgga tctgggagat ctgcaggagt cagagcaatt ataccgccaa
     2101 gctgttactg gtaaacctga ttatgcgcag gcacacaata atctggcgat caatttgatt
     2161 aagcaatgcc ggtttgcaga ggcgatacag caggcgcggg tcgcgctatc ttatcaaaag
     2221 gactatttcg aggctctgtt gtccttaagt acagcttgcc gggctgttgg acagcttgag
     2281 gaagctgaaa aagctgtgca tcgggcaacg ttgattaatc ctgaaagtgc agaagtacgg
     2341 attgatttgg cggacttgct atatatgcaa gatcgttacg gcgatgcgga gattgaactg
     2401 gaacgtgcgc ggcggttggc tccggatacg ccgcggcttt atcttaaact tgctgatgtt
     2461 ttgcaacgcg ggaataaggt cgaggaggct ttgcaggcgg tcgataaagc tgctcagctt
     2521 aatccggaaa tgcctgaggc ctttttgaaa aaaggtatga tctgtcatat ctccaatcgg
     2581 attgaagagg cgaaggagaa ttttgataaa acgctggaaa tgaatccaaa agcagcgggc
     2641 gcatttttgg ccatggcaga acttagtttg tctttgggag aaaaagagca ggcttatgat
     2701 tatgtgtgtc gggcgcagga acttgcgcca gacttaccag cgttgttcct gacgatcagt
     2761 aaaactaaaa aatttactga ggatgatgaa gattttcaaa agatggtcaa gttggaacaa
     2821 tcagcacgga atttcgggat tgatcaggcc agcgctttga attttgcctt gttttcggct
     2881 tatgaagata ttggtgatgg cgataaagcc tttgaacatt tgcaaaaagg caatcagctt
     2941 aaacgccagc ttattccgta tgatccggcg cagcaggagg cgtcttttaa ggcgctgcag
     3001 gaagtgtatt cttctgaaaa tttaaaagcg atggagggta agggttttga aacggagctt
     3061 ccagtcttca ttgttggtat gccgcgttcg ggtacgacac tgactgaaca aattatatca
     3121 tcccatgctg atgtgtttgg tgcaggtgag ctgatggaaa ttggtacgct ggatatggag
     3181 tttggctttc ttagcgcgga gaatgctttg cggcaggggc agcgttatat tgaacaggtg
     3241 aaagcccgtg atccgagcgg gaaagccaag cggattactg ataaaatgcc gggaaatttt
     3301 tctcatttgg gaaaaattgt ttcgatcttg ccacaggcca aaattattca tacacgccgt
     3361 aatcctattg atacttgttt gtcttgttat aagcagaatt ttgcgatggg gcagtattgg
     3421 tcttacgatt tggaagaact cgcgcgttat tataataatt atctggagct gatggcttat
     3481 tggcgtgaag tgcttcctga acggtttata gaaattgatt atgaggaaac ggtgagtgaa
     3541 tttgaacctc aggctcgtgc cttgatcgat tatataggtt tgccgtggga tgatgcctgt
     3601 ttggcgccgc ataagcaaaa acgcgctgtg cttacggcga gtaaacagca ggtgaccaag
     3661 ccgatttata caagctcggt gaagtcctgg aaaaagtatg aaaagcagct cgcgccgctt
     3721 attgaaggac ttgcctccgg tcctgctaag gatttactcg atctttagaa gggaaaatgg
     3781 cgggcgtttt gataaaaacc ttgcgtggag tatatgaaat gctcaatatt catcactatg
     3841 ctggcagact ataaacataa aaatccatcg aaatacctgt tggtcaagtt gtctttggca
     3901 acggtgctga gcgtttttcc aggtgaggtt tctgcgcaaa gcttgaattg tttcgagagt
     3961 ttgatttttg gtgaatttgt tacgtgcgga gcggcgggca gtgtaacggt tcggcccgat
     4021 aattcaacaa cttcgtcgtg cgttacggtt gggtctgcgc cgcagacgcg tgggcgttgt
     4081 gttgttacgc agtctttccc ttttcgtccg attcaggtga atgtatcagg aaccaccacg
     4141 attaatagtg gtgggaattc gatgagtgta acgaatgtta atatcgtgac gaataatggg
     4201 ggaacgggta caactgtgac ggcgccgttt ttgaatgttc ctatcggggc gacgcttaat
     4261 gtcggggcga cgcaggcgag tggaacatat accggcgctc ttggtgtgac cgcaatttta
     4321 caatagttta taagtcgaac ccttaaatac aggttttaag atcatggccg ttagtaaatc
     4381 tattctcaca gtttcagcgc tcgcttttgc tttgatgttt ggcggcgggg tttatgtgtt
     4441 gagccagctt aatgttctgg ccaagccgct tgtggaaaag attgcatctg acgcgctggg
     4501 cgctgatgtg agtattggcg cgatggatat ttctttgccg gaaaagcgtg tgcgtgtgcg
     4561 tgatattcac attgctaatc cggaagggtt ttcaaaaccg catgcgctgg gcgttggaaa
     4621 tatagaggtt ggtttacgct cagtgtcaaa ggggttgatt gattttgaaa atattgatgt
     4681 tggcgctgtg caggtatttt tggaagtgca gcccgatggg acgaatttgc acaaactgaa
     4741 atccggcatc aaaggtggaa aagaggaagc agggaaagaa ccgctcaagg ttattattcg
     4801 ccattttgtt ttggagggtg cgcaggttta tccgtctgtg acgttgctgg atgtgcagga
     4861 tttgaagccg gttaaggttg cgcctgtgcg gttgaacggg attggtgtga aggagaacgg
     4921 aattttggcg cgcgaagcgg tggcgcaggt tatggcgcca ttgctggaga ctttttcgca
     4981 agctgccgga gatgctggat tttatcaggg catgtcgccc ggggcgttga aagagatggg
     5041 catggaccag cttgaggtta tcaaagagca aattcgtcag gaagtcgata atatcggcga
     5101 gggacttaaa aagatttttg attaggcgtc tggattatcc aagcgggcga tttgtgcgcg
     5161 gttgtgttgg cgtgcactga gaatgtgtaa gaaggctctg gcgaagaggt cggctgcagc
     5221 aatgggggat atcccaaggg gcaaggaatt aaacgcgaaa gaaaaaaatc cttgacgaaa
     5281 taggaataat atcctataca ggaagccgta tactttgtgc gatgggctga ttgtccacac
     5341 caccggcgcg aatccgcccg gtccctttga aaaaaacaaa gggcaagcca acagggtgaa
     5401 agaccggctc caaagcacgc gacaacgttg ttgtgatagc gctttgatac cgggcgcccg
     5461 tatctttatg ttttgaaaaa gctaaagaat gcgaaagaac atctttcaga tgctaaaacg
     5521 cgctccttcg gctcgtaacg aaatatcttc gaaccccttg ctatgcctgc tcgcctttgg
     5581 ctatagttaa ggacgcttta ttaatcctat aagaggcgca ccatgcacaa aattttgatc
     5641 ctcaccgcac tgtttttcat cccgttcaca aacgtagcct tcgccgatga aattccggtc
     5701 ccatctacgc tgaaagccgc tacagttttt gctgatcgtg cgactcttac ccgcagcgtc
     5761 aaaatagatc ttcccgccgg taaacatgtc gtggtttttg aaaatcttcc ggcgaatctt
     5821 ttccccgact ctttgcgcgt tgagggaaaa ggaacggcca aagccactct tggcgcactc
     5881 tcgcacaaaa tcgttaacag cgttgaactc acctctgaac gtgaacagga actgaccgcc
     5941 aatctcgaaa ccctcgaaaa ccagaaaaaa attatcgctg cagaaaaaca ggccctgagt
     6001 caaaaacgcc agtttctcga atcgctcgcc gcacgggcgg ttgaaaaaga ggatgaagca
     6061 atcgccgaat ttaatctcaa tacagaaagc tggctggccg gagccgagac aatttacact
     6121 ggaattagcg agatcctgag agcagatctg gaaaaaaata aacagattga agatttgcaa
     6181 aaacaaatca acaaaatcgt gcaagatctg aatcaacgcc gcactggcca gaaacaaacc
     6241 acgcaagtcc gcttgcccgt tgaggccacc ggcgccggca cgcttaccct cgcactagac
     6301 tatcagatcg gcggcgtaag ctggcacccg gtttatgatg cgcgcttgga tacccaaaac
     6361 ggtgcgctcg aactcatcca atacggttcg gtatcccaac gcactggcga ggactggagc
     6421 aatatcgaac tgaccctgtc tactgcccaa cctcaccgcg gcgcaaccgc cccgaacctc
     6481 ccgccgatgt gggtcagcct gtatgaccca actatagcca gacgtcaaag cgtcggtgat
     6541 tttggatatg gtatggctaa aagcgttgtc ccagccgatg ttatggaaac tgaagaacgc
     6601 acagaaatgg atgcttcggc tccaatgcct gttgaagcga tgaaagaaac tcccatgcgc
     6661 actgctacta ttgacaccgg cggtttcacg gctgaataca aaatccccgg cctgtcctcg
     6721 attcccgccg atggcacgca aacaaaagtc ctcatcgccc cgtttgaaac cgagacaaag
     6781 cttgaagtcc acatcaaacc gcaagccaat accaacgcct acctgatcgc caaaaccaca
     6841 cttaagggcg aagccccgat cctgcccggc ccggccttcc tcttccgcga cggcgctttc
     6901 gtcggccaga caggcctgcc tttgctgcgc cccggcggcg aacatgattt atccttcggc
     6961 atcgacgacc agattgaagt cgctcataaa accctcaaag acgaacgcga tgaggagggc
     7021 atcatcatga acaaaaccaa aagcgttgag cgccatacgg tcaccgaaat tcacaatttg
     7081 cgctcaaaat ccgtcaatat tgttctcatg caaatgctgc ccagtgccaa agacaagaat
     7141 atcaagctca acattctcac cgagaaaact acgcctgggc ttgaaaccga tcatgataaa
     7201 atcaaaggcc tgagcgtctg ggattttgcg cttgatccgg gcgcaaaaaa agacgtcaaa
     7261 ctcggctgga aactctcgtg gccgcaggat aaaacgctca cagggttgcg ctaaaagcgg
     7321 agaattttcc tccatgggca aagaacgatt gtcctttaac ctgcttttcc ctacacttct
     7381 tccatggtga acaagaccac gtccaaacaa accctttccc tgacaaaccg tatgcttgcc
     7441 gcattcggtc gcgggtcctt gatcacagaa aaagcccgcc ttgaagcctt tttaaaagct
     7501 tatccgggcg aatattgcgg ctggtccagc gacggcagtg ttgtttactc gcagagcttt
     7561 tgcgatgtgc tcggtatcaa atccatcgac aaacttatcg atattcaagg gcacctcgct
     7621 cccagcgatg ccgctgcatt ggaagggctg ttcgaacggc ttgaaaaaaa tgccattcct
     7681 tttaccactc atgcaaaaag ccacgataaa accaaaatct ttaaaatcac gggctcgcaa
     7741 ggtcaggata ctgaaaacaa agatcgcttt aatattctct ggcttgaaga tgttacagaa
     7801 agttttaaaa cccgcgagac tttcgaacat gatgcccaaa tcaaagccca ccagctcgag
     7861 cagcttcagg ctgcttttca tgcgttgccc caaccacgct ggctgcgcga taagaacggt
     7921 gaaattatct ggatcaatca agcctatagt gatgctgccg ggctcaccat ttctactatt
     7981 ttaaatgagc aaaaggaact ccccgcatcc acacgcaaac tcaaagacaa aaaagacgcg
     8041 tcccttccgc ccggaccgga actggccgcc tgcgctcttt ccagcggtga aaaacaggaa
     8101 acacaggccc atatcaatat cggcggcaaa cgcctgctca tgaaaatcat cgaaatgcct
     8161 cttccagagc tcaatatgac gctcggccta tgcgaagaca tttcccgcat cgaagaacta
     8221 gaaaccgccc tcaaacgcta tcaggcctcc aacattgaat tgctcgaaca actccgcact
     8281 gctattgcaa tttacagcaa tgacgctcga atcgaatttt acaattcagc cttttcccaa
     8341 ctttgggggc tggaggatgg ctggctaaac accaaaccca agcttggcga gatcatggaa
     8401 aaactgcgcg aaacacgccg cttgccggaa caagctgatt ttcgcaaatt caagcaaagc
     8461 tggctcgata tgttcacctc attgataggg ccgcatgatg aaatgctcta tctgcccgat
     8521 ggcagtgctt tgcgtatgtt ggtcgtcccc cattccatgg gcggcattat gatgaccttc
     8581 gaagatgtca ccagccgcct cgaacttgaa tcctcttaca acacccttat tgccgtacaa
     8641 aaagaaacgc ttgataatct cggcgaagcc gtggcggtgt atggcggcga tggccgtctc
     8701 aagctgtgga atccggcctt tggccgcctc tggaacctca atccagaaga cctagacggc
     8761 aagccgcatg tctccaaaat tgtcgaaaaa ctgagcggct tttttaccgc cgaagaatgg
     8821 ccgcggcgca aagaagaact caccgccaaa gcccttgacc gcacgatgca tgaaggccgt
     8881 ctccggcgcg caaacgaaac actgattgat tttaccaccg tgcccctgcc ggatggcggc
     8941 gtactcatca cctatatcga tgtcaccgac acagtgcgcg ttgaaaacgc cctgcgcgaa
     9001 aaaaatgctg cgctcgaagc tgccgaacag ctcaaacttg actttctggc caatgtctcc
     9061 taccagcttc gtacaccgct caacgctatc atgggtttta acgaaatcct tgatcaggaa
     9121 tatttcggca agctcaacga caaacaaaag gaatataccc gcgatacgcg caaagccagc
     9181 gaacagcttc tcgatctcat caatgatatt cttgatctgg caacgcttga agccggctat
     9241 atgaccctac aacgcagtga tatcggcatt tatgacttca tatcaaatat ccagaaacgt
     9301 gtcagcgatt gggctcgcaa agctgaaatc gaagtccaca ttaagtgtgc taaaaatatt
     9361 ggtaaactca gcgccgatga aaaacgcctc aaacaagcca tcatcaacgt gattcgcaac
     9421 gctattaact tcacgcccgc tcacggcgaa ataacactct gcgccaaacg caaaaaagaa
     9481 ggcatagaat ttacagtcca ggatacgggc attggcatcc gcaaggaaga caaacttcgc
     9541 atctttgagc cgtttgagcg cgccgggatt ggtgaagcca attccaaaaa agggcattcc
     9601 ggcgctggac tgggtctttc actggttaaa aacattgtcg cactgcacgg cggcaatgtt
     9661 aatcttgaaa gtgcgcaagg caagggtact tgcgttactc tgttcattcc cttcacatcc
     9721 atcgaaacac ctctcaaact cccgctcgat aaagttaaaa aagcggcgaa gaattgaaaa
     9781 gatgtgtacg gtctattctg gaggcttgcc tgttggtgtc ccatccgctt gaaaacattc
     9841 tgcccaggta atcacgccgc tatcatcggc gcggcgtgca cctctaatga caatttcctt
     9901 gcccgtatcc acgcgggttt tacactcaaa caccggaaag agcgttcgta gcgttacaat
     9961 taacatccgg ttgaggaaaa acggaccata agaacaaaat tcaacgcgat ccgatgcctc
    10021 acctgttttt tcatcaatcc ctccggcctc atccagtcga aaatttgaac gatgacgcgc
    10081 tataattcca tcaggacgca taaattgttc ggtagcgatg gtaccataga ctttataatc
    10141 cgcagcattg cgcaccgcaa agcaaacagg ctcttgaaca atatcaagat tttcattctg
    10201 tgctgcagca atctgcggca ttaaaataag aaaaacaaaa atgaataaag cccgtctcat
    10261 agggccatta tatcacgcaa gcagctcaaa acctaatatg aatccttatg cggcgtacgc
    10321 aaaaaatatt gcattttcag ttatttaaac atatacaaaa cttatgaaac acatcagcga
    10381 taatgaaaaa gatacaagcc gtatcgctgc cgctttggcc gaagatttac agcctggcaa
    10441 tgtacttttg ctgcacggaa cactcggcat gggcaaaacc gttttcgcac gcgcgctcat
    10501 tcgcgccctt accaaccagc acgatataga cgtgcccagt ccaacattca cgcttgtcca
    10561 actctatgac ggcctgacct gcccgattta tcattatgat ctctaccgca tcgaacaccc
    10621 ggatgaaatt ttcgagcttg gctgggacga tgctctggct gatggcatca caatcgtcga
    10681 atggcccgat cgtttaggtc cctacaaacc tcccctttct ctggacatta ccctttcgaa
    10741 tgctgacaat gatccgaata aacgagagat tctgattgag aaaaacgcat gactacagcc
    10801 gctaagtcca tgtccgatag agcgtttatc ctggccgcag ggttcggcaa acgcctgcgt
    10861 ccctacacca acgatattcc aaagccgatg gtcgaagtgg gcaacggtgc aatgattgat
    10921 caggctctcg ataaactcgg cgctatcggc atcaggtcct gcgtcatcaa tacccattac
    10981 aaggctgaaa ttctccagcg ccatctgcaa acaaggcgca ggcctgaagt cattctctct
    11041 cacgaaccgg atctgctcga caccggcggc ggcatcaaag cggccatcag ccatttcgat
    11101 gccccgtttc ttgttctcag tggcgattcc gtatgggaag atgctcccgg ccaaaatact
    11161 ctcgaaacac tagccgcata ttgggatccg gcgcgcatgg acatcctcat actgcttcag
    11221 gcgatcgaaa ccatgaccct tacccacgcg ctgggtgatt atgatctgga cgaagatggt
    11281 cacgctcatc gctccatcaa taaaaccgga cgttatatgt ggacaagcat tcgcatcaat
    11341 gccccggaaa tcttcggaaa cgccccagac gggccttttt catatctgga tttactcgac
    11401 accgcccagg caaaggggcg cctctacggc cttgtccatc acggcgaatg gcatcatatc
    11461 tctacgcctg cagatctcga tgctgtcaat gccgccctga aaaccagatc cggtcaatca
    11521 tgaatgctct aaatgcgcca gctaaaaata tcaccaccat ttccgccggg cagccctttt
    11581 ctcgcctgct ggctgaccgt ctgctcgaag actacgctgg tcgcgaagaa agcttaagcc
    11641 aaacacttat cttgctgcct actcgccgcg cctgccgcgt cctgcaagac agctttctag
    11701 ccctaagaga agccaaaccg cttattctgc cgcgtatgca accccttggc gatcttgatg
    11761 aagaagagct ttccctgtcc atcaccggtc aaaccggcca agatcacgcc tggtccttgc
    11821 ctgccgctct tcccgacttg cagcgtcaaa tcctgctggc aaaactcatt cgccagatcc
    11881 atactgacga tagcctctac agcgcttcgc atgaacaaac cctgaagctg gcgcaggcgc
    11941 ttgggatgtt tatggatcac gtctatactg aaaatctcga tatggccgat ctggctaatc
    12001 ttgtgcccga agacttcgcc gagcattggc aaatcactct aaagtttctt gaaattctga
    12061 gcgagcactg gccaaaaatt cttgctgaaa aaggcgttat tgaccaagct gaccgacgaa
    12121 accgcctcat tcttgcactg gccaaacact ggcaagcttg tccgccgaaa acgcccgtta
    12181 taggcgcagg ctcgaccggc tctattcctg caacagcgcg attgctctct gtcattgccg
    12241 agttgccaga aggtgaggtc attctacccg gctttgacct ggatctcgat gaagacagct
    12301 ggaacgctct tggcgaaacc cacccgcaat ttggttttaa acagcttctc gggcgtatga
    12361 aaattgatcg cgaaacggta aagcccttta aaaacgtgca aaacaacccg cacaataata
    12421 accgtcaaat ccttgccagg cagctcatgc gccctgccga aaccgcacag cgttgggccg
    12481 agattgctga gtgcgcgcaa agctccgctg cgctgaaaaa cgcgctggat gatctttctc
    12541 tcatctcctg ccaacattta cgcgaagaag ccgacgtcat agctctgctc atgcggcaaa
    12601 cgctggaaac accggacaaa acggcctgtc ttatcacgcc tgatcgtgaa ctcgccgctc
    12661 gcgttgccgc ttcttgccaa cgttggaata tcacaatcga cgattcagcc ggccaaaatc
    12721 tccgccattt acctatcggc atattcatac tcgctgttct ggaagctata agcgaagatt
    12781 tttctcccat cgcgctgctt agtctgctta aacaaggctt ttgccagatg ggtaaaaacg
    12841 tacaggcgca tgtctgccaa accacagcgt tagactttgt cctgcgtggc aataaaccgt
    12901 ctgcgggttt tacgggccta aaaaagcata ttgaaaaaca agagagtttg gacgaaaacc
    12961 tcaaagccga agtgctgggc ctcttgtctg aactcgaaga catattcacg cctcttcttg
    13021 cgctgcaagc tgagccgaaa acattcaggc aaatcctgca aactcatctg catatatgtg
    13081 aatctttatg cgccagtgaa gaccgggaag gcgcacaaaa tctctggcac ggcgaaattg
    13141 gccgccatgc cagccgcttt ttcagcgaat tatttgacca tgctcacgcg ctggacacac
    13201 taaatttgcg cgattatgct gcagtgctcg cacatttcat gaaccaaacc cagatccgcc
    13261 cggcctttgg cacccacccg cgcttgcaaa ttctcggcac attggaagct cgcctgattg
    13321 atgctgatct ggtcattctt ggtggcctca atgaaggtgt atggccgcct gaaccggcta
    13381 ctgatccatg gatgtcccgg cctatgcgca aagaattcgg cctgcccgca cctgaacgcg
    13441 gcatcggcct ggccgcccat gatttcgtac aaggcctgtg cgctccttgt gttatcctca
    13501 cccgcgccga aaaacaagat ggtgcgccta ccgtcccttc gcgctggttg caacgactgg
    13561 aagccgtcct gcatgcagca aatcttgaaa ccccgcaagg cggtaatatt ctgcactggg
    13621 caagattgct ggatcacaca agcgaagtga aaccgattga catgccttct ccttgtcctc
    13681 ctgtcgagat gcgcccgcgc catctttccg tcacaaaaat tgaaacctgg ctgcgcgatc
    13741 cttatgcaat ctatgcccgc tacatccttg gtctcaaaat cctcgatccg cttgaaaaac
    13801 ctgtcgatgt ggcccagcgc ggtcaactcc ttcataaaat cctcgaacgc tacgttcttg
    13861 aaacgcgcga tgctctgccc gatggtagcg ccgctctcgc cttgaaaatt gctcgcgaag
    13921 aaatagaccg gcgcggcgat gatccgcatc tgtggagttt ctggtggccg cgctttgcaa
    13981 agacggccca gtggctggat tctcacgagc gcggcttgcg tgaagctacc cgtaatctgg
    14041 ctgtcgaagc tcgcggtgaa atcgacatca aagcgccggg cggcgatttt aatctcaccg
    14101 ctattgccga tcgcatagac ctgcttccgg atggcaaagc cgcgctgata gactataaat
    14161 ccggcggcac atacagcgcc aaagccatcg aaaatggcac cctgccgcaa ctcccgcttg
    14221 aagcccttat tctcgaaaaa ggcggattca aaaacctgaa accatatgta accggctcac
    14281 tacaatactg ggttctcaat ggcacaggcg acggcgcgaa gctcacaatc tatgacaatg
    14341 ctttagatga actcgtaaaa cgaaccgccg agaaccttac cgcgctcatt accagttacg
    14401 ataaccctga tagggcctat ctatgtcagc cccgcccgga tcaggctccg cgctttaatg
    14461 attatgaaca tttcgaacgc gttaaggaat gggcaatcag cgctgactct gaagaaacgg
    14521 aggccgcatg agcgcgcagg tcgcacccag gaaacaaacc gaaaccgttc gtgaagtcga
    14581 acccaatgtt ttgcaacgca gggcttcacg ccccgacgcc tcagtctggg tcagcgcctc
    14641 agccggaacc ggcaaaacca aggttctgac cgatcgtgtt ttgcgcttgc ttttgccgcg
    14701 tgaagaacgc cccggccaga ttatccccgg cgccccggct cataaaatcc tgtgcctcac
    14761 cttcaccaag gccgcagcca gcgaaatggc cctgcgcatc tcaaaaaccc tcagcgcatg
    14821 ggctatcatg ccgcttgatg gaccggaagg cacaaaaacc ctgtatgccg aattgcgcgg
    14881 tcttctgggc cgtgcgccca gtacaatcga aatcaaagcc gcacgcaaac tttttgccga
    14941 cacaatcgat gtgcctggcg gtctcaaaat catgaccatc catgcctttt gccagtcgat
    15001 cctcggccgt ttcccccttg aagccggtat caacccgcac ctcaccattc ttgaagattc
    15061 cgccagtgaa gcgcttttaa cagccgcgcg caaccgcgcc ttcgccaacg ccgaaaaaca
    15121 acccggctca gaactcggac aagcccttga gcgcatcgct gccaccatca acgaagatca
    15181 gttcctcagc ctgctcaatg atattactcg tgaacgtcgt caacttgcgc gcctgaaagc
    15241 ccgttatttt gaccttgagg gtctctacag cgctctatgc gtacatttgg gcattgcccc
    15301 tcatacaacg cctcaaaatc ttctcaacga tgcctgtagc gacgaatcct ttaacgctaa
    15361 tgcgctgcgc agtgcagcgc tgaccatgca aaagggcaaa ggccaggacc aaactaacgc
    15421 cgaaatcatc tttaactggc ttgccgcctc tacagagcaa cgcatgcagg acttttccct
    15481 gtatcaaagc gccttcctga aaaaatccga cgggcagatt aaagcccggc tggcgctaaa
    15541 aaacagcatt gaggccaacc ccgaaatcct tgacattctc acccgcgagg gagagcgcct
    15601 catcgccctg aaagacgcta tggccgccag ctccagcgct gcacttaccc gtgatattct
    15661 gattttaggg gaaagtattt tagacgaata tgaaattctc aaaaccggcc agggcgcgct
    15721 tgatttcgat gatctcattc tgaaaaccct cgacctgctt agaggtaaaa gcatggggct
    15781 aaacgctgtc aacgctggaa gttgggtcca ttacaaactc gaccagggtc ttgaccatat
    15841 cttgatcgac gaagcccagg acaccaatcc agaacaatgg caaatcatag aagctctttg
    15901 tgacgagttc tacagcaatg ctcctgataa caatatgaca cgaaccgtat ttacggtggg
    15961 tgatgaaaag caatctatat acagcttcca gcgcgcttct cccgaagaat tcgcccgcat
    16021 gcaaagcgat ttcgaacgta aaaccaaaaa tgcgctgcaa acatgggaca gcgtccccat
    16081 gaacatctca ttccgctcta ccagcagtgt tctaaacgct gtcgattcgg ttttcgccgc
    16141 tgcacacact cgcaaaggtc tcagcgccct gcctattgag cacacagcct ttcgccgcgg
    16201 ccaggctggc cttgtcgaac tttggcccct ctttgagagc gacgagtgcg gccagacaga
    16261 tctctggact ccgcttacca gcgctgaaga agctcaaact ggtcgcaaaa aactcagcgt
    16321 tcatatcgct aaaaccatca aaacatggtt aagcacccaa gaaatactcc cctcaaaagc
    16381 gtgtcccatc cgccccgggg atattatgat tctggtgcgt acccgctcaa accttgtaaa
    16441 ccagatcgct tacgagctca aaaacctgaa tgttcctgtc agcggtctgg accgcatggt
    16501 tttgtctcag gaacttgccg tacaagatct gctggcggcg gcagaatgcg cgctccagcc
    16561 tctggatgac ctgacgcttg ccagtgttct taaatctccg ctcatcggcc tcaatgaaga
    16621 cgaaatcttc aacctcagcg cctaccgcgg caaaaaaagc ctctgggaaa gtttgcaagc
    16681 cagcgatcat cagaaaatca ccgcctattt aaaaaacctg cttaccttgt caaaaaagac
    16741 aacgccattc gaatttttct cccacattct tcaatccccc tgcccgacag acacaatcag
    16801 cgctcggcgt gccatcatgc ggcgtttagg aaaggatact ctcgatcccc tcgacgaact
    16861 cttaagtctc gcacgcacgg ttgaaaaaca atccccgccc tctttgcaaa aattcatcca
    16921 tgacagacgt gccagccatc aggaaatcaa acgcgagcat cacagcgata acggcgaaac
    16981 tgacggcgaa gtgcgtatca tgaccatcca cggctccaaa ggcctgcagg cccccattgt
    17041 tatcctgccc gatacgatca ccagcgcggc gaaagaggct gcccgtgctg aaaaacgcct
    17101 gctctggcca aaccagaccg gccttaacct gccgctatgg tctccacgca aagaatgcga
    17161 ttaccaggcc tatcgtgaag ccatgggcgc gcttaaccag cgtcttgacg aagaatatcg
    17221 ccgcttgctc tatgtcgcca tgacccgcgc cgaagaccgc ctttatattg gcggtgcgct
    17281 gggcaaaagc caaagccctg aaaaattgcc cggtggttgc tggtacgatc ttattgcgca
    17341 aggcctgcaa aacctcgaag atatcgaaga cctgaaaaac ggcggtttgc gcctgaccaa
    17401 tccgcagatt catgaaccgg aaaaacccaa agtaccggga aaaaccgctc cactgataga
    17461 gcctacgcca caatggctgc acacacatcc gccatcagaa agtcagacaa caaccattat
    17521 ccgcccctca catattgccg acactgccat ctccccactt caaggcacca acaatcaacg
    17581 ttttgtgcgt gggaacctga ctcacaagct gttacagctt ctcccgacta tccctcggaa
    17641 aaactgggaa aataccgctc aggctttcct gaagcgctac gcaaaaaccc taagcgctga
    17701 aattcggggc gaaattgcct gcgaaacact caaagtttta aatcacccgg attttgctcc
    17761 attatttggt cccggttcca tggccgaagt cccgatcagc ggccatttag aaggaaaagg
    17821 tctcatcagc ggccaaatcg accggctcta tattgatgaa aatacaatct cgattatcga
    17881 ttacaaaacc aatcgccctc caccggaaaa tccggcgcaa atcccggaaa tctaccgcag
    17941 tcaaatgcat acctacgccg ccgtgctcaa aaaaatctac cccggccgca ccatccacgc
    18001 ggctttgttg tggacagacg gcccaattct catgcccctt gatctcaaaa tcatgtagcc
    18061 tatagtcaat gaagtgctca tattgcgtag ttttgaaagg ataaaaacat gcccagcgta
    18121 gccgttacag attcgaattt tgatagtgaa gtgctccaat ctgacaagcc cgtcatcgtt
    18181 gatttctggg cagagtggtg cggaccttgt aagcagctct cacccgtaat tgacgaattg
    18241 gccgaagatt taggcggtgc ggtgaaagtc gtgaaagtga atatcgacga agccccggaa
    18301 gcgccaacca aatatggcgt acgcggcgtt ccgactttga tgattttcaa ggaaggccaa
    18361 gtggttgaca cccgcgtcgg cggcatgccc aaatcccagc ttcaggaatg gatcgaaggt
    18421 caggtttaaa aataagaagg aagaagataa gagggaagag ttcttccttc ttacttctgg
    18481 acttccgagg acagcaccat aataatctga cgtccttcca ttttgggctg tagatcgacc
    18541 ttgccgacat cggcaagctc ttcggtcatg cgcttaagca ggtcaaaacc aatatcctgg
    18601 tgagccattt cacggccgcg aaaacgcatt gtcaccttaa ccttgtcgcc atttttcagg
    18661 aatttccgcg cactgcgcat tttcacatta tagtcgtgct cttcaatgcc ggggcggatt
    18721 tttacctctt taacatcaac aatcttctgt tttttccgtg cctcagaagc ttttttctgc
    18781 tgctcgtatt tatacttgcc aaaatcgaga atcttgcaaa ccggcggaga tgcattcggg
    18841 gaaacctcaa ctaaatccag accggcttct tcagccagtt ttatagcctc cgcaacagac
    18901 ataacgccct gcatttcacc atccgcatca acaacgcgaa cttctgaagc tgtaatatca
    18961 tcgttaatcc ggggcccgtc atctttgcgc ggcgggcggt tattcactgg tctggcgatg
    19021 gtttagtctc ctgtaattgt taaaacgtta ttcaaaatca taatttataa tggtttggaa
    19081 atcattaata cggatttttc gcttaattcg caagcgtttc ccatcggcgc acacaacgca
    19141 tcaattcagg gtccatatag agtaattcag atacgcggca aaacagcccc acacaattgt
    19201 cggaataacc aacaggcttg cccggcgctc aacagtccaa gccgcagcaa caaaagccaa
    19261 catggccacc aagtacgcca gaatccagaa gaaccccaac agtatcagat gaaattcaaa
    19321 aaatatgaat gtccatcccc agtttaaaaa catttgcatc caatacagat tatgcgcttg
    19381 gttaccgccc ggtttctttc gtgcattcca caccagccat ccggccagcg ccagcaaagc
    19441 atagagcgtg ctccagacaa tcggaaacac aatatcaggt ggattgaacg gcgatttaat
    19501 cagcgtgtcg taccagccca tattctggcg cgtcagtaga ccgatcgccc agccaatcac
    19561 ctgaaacacc aaaatccata atgttggagc gatatatttc atatttcttt aaaacggagg
    19621 ttgagcttct tgcactaacg cagagattgc atcatccaga ggatcaacgt tctggtcatt
    19681 cgagcccaac cggcgaatag ccactgtgcg ctcttcggct tcacgcgcac cgaccacaaa
    19741 caaattcggt actttggcaa cgctatgctc acgcactttg taattaattt tctcgttgcg
    19801 aatatccaga tcggcgcgta tgcccgccgc tttcagagcc gcgcacacat cctttgcata
    19861 atcatcggcg ctgccggtaa tcgttgccac cacgcactgc accggcgcaa gccataacgg
    19921 caatttcccg cccgtgcttt caatcaatat gccgataaac cgctccagcg ttcccaaaac
    19981 tgcgcggtgc agcatcaccg ggcggtgctt ggccccatcc tcgccaatat agcttgcatc
    20041 caggcgctcc ggcaaaacaa agtcgagctg taatgtcccg cattgccagc tccgcccaat
    20101 cgcatcacga atatgatatt cgaccttcgg cccgtaaaat gcaccttcgc cttcttcttc
    20161 ctcaaattcg aggccagctt cattcagcgc cttacgcaaa ccattttcag ccttgtccca
    20221 aacctcatcg ctgcctgcac gcatttccgg gcgcaaagcc agcactacac gtacatcctc
    20281 aaagcccaga tccgtataaa cgcgcttgat caggttacaa aagcgaatcg attcttcggt
    20341 aatttgatcc tcgcggcaga aaatatgcgc atcatcctgc gtcatttgcc gtacgcgcat
    20401 cagcccgtgc agcgctccat gcgcttcatt tcggtggcaa cagccaaact cagccagacg
    20461 gatcggtaaa tcgcggtagg acttaatccc ctgattaaac acctgcacat gcgccgggca
    20521 gttcatcggc ttcaaggcca tcagcttttc aaacgccttg tccgatacca ccggcccgtc
    20581 atcttcgccc gaaggaattt catcgggcac gacgaacata ttttcgcggt atttccccca
    20641 atgcccggat ttttcccaaa gcttgttatc aatcaattgc ggcgtcttga tctcattata
    20701 gccatcatca ttcacccgcc ggcggatata atcttccagc gcatgataaa tcacatagcc
    20761 tttgggatgc cagaaacatg acccttgcgc ttcgggttga aaatggaaca aatccatttc
    20821 attgccaagt ttgcgatgat cgcgtttttc cgcttcttca agctgcgtca gataggcttt
    20881 gagttcctta tcatcgcgcc aggctgtgcc gtaaatccgc gtcagcatcg ccttgtcgct
    20941 gtccccgcgc cagtaggccc cggcgacttt caacaattta aatgccgtac cgacatggcg
    21001 cgttgttggc atatgcggcc cgcggcataa atccagccaa ccattctccc cttggccctg
    21061 ccgataaatt ttgatctctt cgccttctgg caaatcctcg atcagctcgg ccttgtaata
    21121 ctcgcccttg tctttgaaat aggcaatcgc tgcgccgcga tcccaaacct cgcgctcgaa
    21181 aggcgcgccg cgttcaacga ttttgcgcat ttctttttcg atagcctcaa gatcttccgt
    21241 gctgaacggc tcattgcgcg caaaatcata ataaaacccg ttttcaatcg ccggaccgat
    21301 tgtcacctgc gtccccggaa aaagactctg cacggcctgc gccatcacat gcgctgcatc
    21361 atggcggatc atctccaggg caatgtcttt atcatcatcc ccgttgcgct taaccaaagc
    21421 gatagaagca tcgcccgtaa tcggcagtga caaatcgcta agctcgccat caatttgcac
    21481 cgcaatcacc gccttggcca atgatttcga aatatcttcg gcaatgtcga gcgccgtcac
    21541 accggacgca tatgtgcgca ccgctccatc aggcaatgta atttgaattt gtttttctgc
    21601 tttttgttcc ataatcaaag gaaactagcg ttcattcatg gcgttgtcga gagtctttat
    21661 aaagggtttt agcaaatact gcatcactgt cttcttgccc gtcaaaatat cgacgctcgc
    21721 caccatcccg gtcgtaatcg gcagcacctc gcctttgcgc accagctcgg ttttatcggt
    21781 gcgtaatttc acccggtaaa aactgttacc ttcctgatcc tcaatcgtat cggctgaaat
    21841 atcaaccagt tcaccgtcca gcccgccata aatggaaaaa tcataggctg tgagcttaat
    21901 caccgccttt tgccctggaa agataaaggc ccggtccttg gggctgactt tcgcttcgac
    21961 aataagctga tcatccttgg gcacaatctt gatgatgtcc tcacccgggc gcacaacccc
    22021 gccaatcgta ctcaccgtaa tttcctgaat agtcccgttc accggcgatt tcaaaacagt
    22081 gcgtcctttg cgctgtttca gcgccgccag ccgctcctta atctcattca tctcgatcag
    22141 cttggctgaa agctcggctt gggcttgcgc cttcatattc gtctccaact cggcaatccg
    22201 cccgcgcgcc tcttcaatcg aagctttgat ccgcggcaca ctcgacgtat agctgttaag
    22261 ctctgcattt ttctctttaa ttgtgcgctc aagctgcagc aattccatct tcggcgccga
    22321 gcctttttca accaacggac gcaccatacc tgcttcctgg cgctgcaaag caatcacccc
    22381 gcgcgtatcg gcaatgcgcg tgcccacttc acgcagctcg gattcacgtt gactcatctg
    22441 ctgttgcaac acgttacgtt gcccgttcat cgactgctga ttagcccgaa acgcgttgag
    22501 ctcttctgta acgctttgcg gtgcgccctt cataacttct tgaggaaact caaccgtgcc
    22561 tttgccttcc gcttcagcgg aaagccgcgt aatggaggcc agcaaaccca gatagcgcgc
    22621 tttattggcg cccaaatccg actccgcttc aatctgatca agttgcatca aagcctgccc
    22681 ggcctcgaca aggtcgcctt catgcaccaa aattttctca acaacaccgg catccaggct
    22741 ttgcagcgct tgaatatcgc ttgacgggat caccttgcca ttcccgcgcg tgacttcatc
    22801 cagcgaagca aaatttgccc aaacaataaa aattacaaaa aatgatgtga tcgccataaa
    22861 caaaagatgc ttggaaagcg gcatatcatc atcagtatca aacacggcca aagctcgcgc
    22921 atgcaccttt ccggcctgtt ccttggccca gctatcacgg acgccttgcc attcgtcggt
    22981 atcattgatc gtttttttat catcataaat cggtcggccc tgtcccgatg gcgaggccat
    23041 tttaaactcc ttgggcaatc tggatttctc atgctttttc gaaggcgcat tcttcctggt
    23101 tttttttata ttcttgctca tatttacacc tgtgtattct ctgcttctgt cccatactgg
    23161 cgggcctgca atttacggat cacttcatct ttcgggccat acgccaccaa acgtccgcga
    23221 tcgatcagga tcagtttatc aaccagatcc agcatcgcgc ccttatgcgt aatcagcatt
    23281 acggtcttat ccctacaaat ttcatcaaga cgtttcttga gacggttttc tgaagccggg
    23341 tccatcgatg ccgtcggctc atccaaaacc aaaatcggtg ggtcataaag taaagagcgt
    23401 gcaatcgcga ccgcctggcg ctgacccccg gaaagtgctt ccccgcgctc acctacttgc
    23461 gtatctagcc cggcttgaga atctttcaaa aattccatca cgcctgaaag ctgtgccgcc
    23521 cgtaacacgg cccggtccgg cgccgtttca tgccccattg tgatattctg gcgtacagag
    23581 ccaaaaaaca gttgcgggct ttgttgtacg ctcccgacat tccggcgtaa atcacccgga
    23641 tcaatctggc gtacgtcggt cccgtcaatc tgcaccgagc cgcttgccgg cgcataaagg
    23701 ttgatcaaca accgctccag cgtcgtcttc cctgaaccaa ccgcgccaat aatcccgacc
    23761 ttctcacctg gctcgatcgt aaggctgata tggttaagtg ccggcgtaga ctgccccgga
    23821 taatgaaata caacatctcg aaactcaagt tgcccgcgaa tatgcggtaa agttacaaaa
    23881 tgcttgcccg cagggcgctc caccggcttt ttcatcaaat catcaagctg cttcaacgcc
    23941 tcatgggact gactaaagcg cgtcatcaat ccagcaactt gcgcgagcgg ccccatcgcc
    24001 cggcccgata aaatcacgca tgcaatcaat ccccccatgg acagcgaacc ggcggcaatc
    24061 aaatacactc cgacaattac aatcaccgcg cttacagcct gttgaacaaa catcgcccag
    24121 ttctgcgcga acgccgcaat ctgccgcgat ttaacagacg tccgcgatgc cttatcggta
    24181 agctcttccc agctacgctg cgtatggcct tctgcggcct gcactttgat cgtttccagc
    24241 ccgataatcg tttcgaacag caaagcgttt ttcaacgcac tttcctgcaa agacgctttt
    24301 atcaccttct gcaaaggctt ttgcaggaaa aaacccatcc cgacaatcag cggaatggct
    24361 gcgagcggta caaacgcaat cggcccgcct aaaacagcga taatcgcgat aaacagcaat
    24421 acaaaaggca gatcaatcag cgctaccatc gtcgcgctgg taaagaaatc gcgcagcgtt
    24481 tcaaactctt tcatattggc agccagtacg cccgcactgg ccgggcgcgc ttccatactc
    24541 atccccatca tctgttcaaa cagtgctgaa gaaatcacca catcggcttt gcgccccgca
    24601 taatcaagaa aatgcgcccg caaattgcgc agcataaaat caaagaaata aacgatcaaa
    24661 atcccggcgg ccaaaaccca tagcgtttca aacgcattat tcggcaccac ccggtcatac
    24721 acattcatga taaagagtga tccggcaatc ccgaacatat tgatcataat cgccgccacc
    24781 gcgacttctt tatacagcgt tttgttcttc caaaatgccg accagaacca gtcccggccc
    24841 gtatcaattt ccgccggccc ggcccggtca tccacccgcg ccaccggacg aataaaaaac
    24901 gcatagccgc tgtaaagatt tttaagctgc tctaatgtga gtttttggcg ctcatcggga
    24961 gtctcgggaa actggacaat aaacgctgtt tcgggatgaa taacaacatt ctgattatcc
    25021 cgttttttcg gcgtatgttt ttcaggatac ttaatgtccc ataaaatgca tgcctggcct
    25081 tcttccaatg ccagaatgca tggcaggttc ggcgcaatgg ccaaagaatc cagcgaacga
    25141 tccgcaagat gcgcctgcat ccccgcgcgc tcagcggcgc ggataaacag cgatggcgta
    25201 atgcctttat tgggaatggg caagcctgcc accaaagcgt cattgctggt gcggcgccca
    25261 taatgcccgg ccattatacg cagacaatcg accagcggca tccggatcgc catccggtcg
    25321 gcctgcaacg gccatggatc gccttcttta tcaacgatgt ttacagcaac gccgttatca
    25381 ggtgcgtctt gtggttcctg cacgtccttc gattccttcg aactcttatc attcaccatt
    25441 aaaaaaggcc tcttcgctta gggaaaagag gccttgtttg aaacataccg gcatacagag
    25501 ccgccgtgcc tatctggcct ccatcttctt ggatgtgctc cagctatcat cagcagccac
    25561 aacactttct ctaggatact cgacaccaag ggtcgggagc aagctgcctt ttaacgccat
    25621 caagcgataa acggcaaaca tttccagaaa ttcagcatta atcgtgttgg aacgcgaaac
    25681 aaacagctca ttttgtgcat ccaaaacatc caaaagcgtc cggcggtcca aattaaactg
    25741 atctttatac gcttttacaa cctcaacatt ggcatcagcc tgcgctgcaa actcgcgcgc
    25801 gcgttcaccc gctgatacca ttgccgccca cgtctgacgc acatcattct caacctgacg
    25861 agctgcttcc gcgcggcttt ctttagactg ttggtgacga tgcaaaaact cgcgtgagcg
    25921 cgccatatcc gcgccgccgc gatacaaatt ccagttcaca accaccaaag cagatgcgct
    25981 tgtatccgta ccctcaacgc cattaatatc atgtccggtg cgtccattga gttgaaaatc
    26041 aacctgcgga tagaaggtcg agcgtgtttg atgcgcctcg gcataggcca cttcaatatc
    26101 ggattcgaaa atatctaaag tcgggctgta tgccaaaact ttctcaactt cctgttctgc
    26161 acttgcgctc agaacatcat aaggaataac cggcatagag agttgccctg gcgcatcacc
    26221 aacatcggaa cggtactggc tttccgcatt gcgcaaagcc tgacgcgtgc tggattctgt
    26281 cgctttcgcc gccgcaaggc gagctttggc ctgctccata tccgcttgcg ttgagcgtcc
    26341 ggcatcaacg ccatcttgaa tttgatcaag aatagaaatg tgatcggcca cattctgacg
    26401 tgaaattacc agcaattgac gctgacgcag cacttcaaga taagactcaa caatagaaag
    26461 cccgaccagt tcagccgttt cgcgaacgcg gttagcagaa gaatttaccc gcgctttttg
    26521 gcgctcaacc tcatacttcg tatcaaatcc gtcaaacaac atttgtgtca gcgtgaggga
    26581 gacttcttta cgcagcagat tttcttcatc atcactgtcc gttcctccgc gcgttgccgg
    26641 atcatcacta tgctcaaaac cagcatcagc acgcatatca atagaaggga aatacagagc
    26701 ccggccctgt ttaagctctt catccgttgc ccggcggctc gccgcaacaa cgccatattc
    26761 cggatttgta gatacgcccg ttgccacagc atccttcaaa gaaacgctca aagactggtg
    26821 caaagcttca tcctgagata aagcagagcc agcgcttaaa ccaagcaagg ccacacttga
    26881 aaccgtcagg cacaacatac gaatagagtt tttctttaaa atcacgggac tgttcctctc
    26941 actcataaaa attcttaaaa gctcttgggc tgttagcata cttatccaca gcctgaattc
    27001 ttcacagtta tgcctctttt ctctacaaga tgcaagcgct ttgcataatt tttatcgcct
    27061 gaaaccctgt atgaaggctc atttttgcaa cgcgtgttta caaggatggt aaaaacccga
    27121 ccttctataa agaaaaccgg gttttttgga aagaaccgca aaccttaaaa agtctgcatt
    27181 tctgttaaga acctttttat acggttgttt caccattatt ggtggccaat gtcagatcca
    27241 gacctgtaac cgcctccaaa acagcaatct gcgtcgcatt cgcaatattg ccactaccat
    27301 tcgcatcaac ataaattact gtattaccag ccacttcggt agcgaaaaca aagtcgttaa
    27361 tgctgtcttg caaagcgtca taaccgttca gcaacgcaga gacatccaga acgtcgcctt
    27421 cagcaacatt aaagtctttg attgtatcta caccatcagt gatcgcctca aaaatgaagt
    27481 catcctggcc cgatccgcca tacagcgtat cgctaccttc accgccggta atgcgatcat
    27541 tgccttcctg accgaaaata acatcatctc cggccccacc ggtaatcgta tcatcgccgc
    27601 cctgacggcc ctggccttga gtgttcacag actcaacagc caaactttcc gcattggcac
    27661 gaatgtaatt gatcgtatca gcgcgggtcc agccagtatc actaccttcg ccgttttcaa
    27721 ggcgctcaaa gacctcaaaa cctgcaccgt cattcgtggc aaggccttgt gtatcagcca
    27781 gatcatccgt attcagtgtg tcaccaaaga ttaaatcata ccggttgcca ccctgaatat
    27841 cgtcgttgcc aacactgccc agcaagttca atggattatt gcctgccaga acagcctgca
    27901 gatcattggc atcatcaata taagtcgcgc ttgcgtctga atcgatcagg ttgaggtttt
    27961 gattgtcagg cacatcaata ccgacagcca caacatcatc actcagagct ttcaaagcgc
    28021 caacctcgtt gctgtcatcc ccaaacgtac cgtcacttgt tgttacggtc gttccagtga
    28081 tttcagcgcg aataatcgca tcttcttctg ccgcgttacc cggaggattc agaacagtgc
    28141 cctgatcgtt cacatagcgg tttggctcac catcagagat gaaatacgtc gttgtaatcg
    28201 catttccgcc caaaggttca gtaccagata accatgcatt ggctgcttgc aaaggagatt
    28261 cgtagtttgt caagccagtg ccggtttgtg catcaagcca tgccgtcaca tccgctagcg
    28321 cggttgtact tgcaaaatca actgtaaatg ttgattttac gtccgtagaa aaagaaacga
    28381 gatgaacctt gatttgaccg ttctgataag catcaaactg agccatctgg tttttcacag
    28441 cctcaagcat aatttcaacg cggctggtta caccgtcagc cggattattt gtgcccattg
    28501 aacctgacgt atccagaata taaacaaagt tataatcctg cgtctggttt acaacctgcg
    28561 agccgccata atccccaacc agaacgtccg caccggcgcc gccgataatg gcttcactac
    28621 cgctgccaat aacccacggt gtagtctgac cgtcaatgtc atcgacatta ttaccgacaa
    28681 taaacaactc cggcacatcc gcaaccgtaa ttgtcaaaag cgcactatca ggatcgccat
    28741 cataatcagc catcgtgtaa ccaaacacat cctgcacctg agtggtgttg gtattatttg
    28801 acacatagct atatgaacca tccgcattaa tgctcagcgt tccatactgg ccgttaatca
    28861 cgctcggcgt accagcaaca accggaacat ccgcaccgcc aaagctgaca ctgatcacgc
    28921 tcgccggaac atccgcaccg ctatcatcat tagccagaac attaccattg attgtagtct
    28981 gaccctgatc aaccagagcg ccatcatcaa cggccacagg cccgtcatct ttcacattca
    29041 cggtaatcgt gcccgtatcg gtatcaccat cgctatcctc agccgttacg ccaaaggtca
    29101 gcttgatcac atcatccgga ttggtttcat ccgcatgatc taaagtcccg atcaatgtaa
    29161 atgtgtaagc accggttgtt tcattcaaca ccagcgtaaa gatcgtatcc gcgccggctt
    29221 tcccaacata gctgttgcca accacactca ccgtgacagg aacgccgcca gaagtgagct
    29281 gattattctc agcgcccata aagccgaatg ttcccgcgcc cgtaactgta aatgttcccg
    29341 gaccatcatt gccgaagtta ccatcaacaa cattagaagc ttcgtcagtc ggattaagat
    29401 ctgtctcatc aacgctgacc tccgtcgggc caacctcggg acgtgtatcc acatcatcga
    29461 tagaaatcga caaattcgcc gtatcgctat cgccgtcaaa atcggtgagc gtgtaggtga
    29521 agctttcctg aaccgcgttt ggattcgtgc cattagacac atatgtataa gagccgttcg
    29581 cattgatcgt gagcacacca tactggccgt tgacaactgt aggaatgccg ttcacaaccg
    29641 catgatccac gccgttaaaa cgaacatttg tgactgtggc gacaacatct tctccgccgt
    29701 cgtcattggc cagaacattg ccgttaacag tggttgtgtt ttgtgcgacc gtgcctgtat
    29761 catcaacagc caccggaccg tcatcataaa cgttcaccgt gataaagccg gtatcggtat
    29821 cgccgtccgc atcttcagcc gtgacgccga aggtcagttt gatcacatca tccggattgg
    29881 tctcatcggc atgatccagt gtgccgatca gcgtgaacgt gtaggctccc gttgtttcat
    29941 tcaacaccag cgtaaagatc gtatccgcgc cggcctttcc gacatagctg ttgccaacca
    30001 cgctgatcgc tacaggaatg ccgccggagg ttaactggtt attctccgct ccgataaaac
    30061 cgaacgttcc cgcaccggtc accacataag ttcctgggcc gtcactgccg aagttaccat
    30121 caacaacatt cgaagccgaa tctgtcggat taagatctgt ctcatcaacg ctgacctccg
    30181 tcgggccaac ctcgggacgt gtatccacat catcgataga aatcgacaaa ttcgccgtat
    30241 cgctatcgcc gtcaaaatcg gtgagcgtgt aggtgaagct ttcctgaacc gcgtttggat
    30301 tcgtgccatt agacacatat gtataagagc cgttcgcatt gatcgtgagc acaccatact
    30361 ggccgttgac aactgtagga atgccgttca caaccgcatg atccacgccg ttaaaacgaa
    30421 catttgtgac tgtggcgaca acatcttctc cgccgtcgtc attggccaga acattgccgt
    30481 taacagtggt tgtattttgc aaaaccgtat tgccgtcatt cacagccacc ggcccgtcat
    30541 ccagaacatt cacctgaata aagccactgt cggaatcccc gtcactggca tttgctgtta
    30601 cgccaaagct gagcgtaatt ggatcgttcg gatttgtcgc atctggatga tccaaaacac
    30661 cgatcagcgt aaaggtgtaa gcaccggtcg tttggttgag aatcaacgta aagatgattt
    30721 cagtgccaac tttaccgaca taatcattac ccacaaccgt aaccgttaca ggctggcctt
    30781 gtgaagtcaa aggcacagac gagctaaatc caccatcgct gtcataatca acggtaacgc
    30841 caggcgcaaa attaccggtg acaacaccgg aatccgtgtc cgtcggattc agatcgcttt
    30901 catcaaccag caactcatcc gcatcgatgc tcaacggcac attgcccttc agggtcaatg
    30961 tcgcttcacg gctatcgtcg tcatagtctg ttaatgtgta aacaaactgt tcaacaatat
    31021 cgttcttcgc actaccgctt atcactgtgt atgtgtatga accatcagca aaaattttca
    31081 atgttccatg atcaccatca attgttgcac cattaacagg atcgacaaca acgactgtgc
    31141 ctttgaacgc aattttggtg acagtatttt gaacatcttc gctcaaatca tcggtcgcat
    31201 tcggaccgcc gttttcgccg gtaatcacgt taccggttgc gctgcctttc agagggttcg
    31261 tgccctgcaa agctgcatcc agatcattcg ggtcctgaac atccagcgca ttgccatcag
    31321 aatcgatgac acccagacgc gccagtgttg tgccactcac accaattcca acgccgatta
    31381 cttccttgct caaagactgc aaagtcccga cttcgtcact attgtcacca aaggtgccgt
    31441 cactggtcgt tacactcgtc ccggtgatct cggcttgaag aatcgcttgc tcctcggcat
    31501 ctgtccccgg cggattgacg acattaccca aatcatccag ataacgattc ggcgcaccgt
    31561 cactcacaaa atacgtatac gtatccgcgc caggaatcgg atcattggcg cttgcaccct
    31621 gcaaccagtc gatcgctgaa gccagcggcg cttcatagtt cgtcaagccg tcggcattta
    31681 gcccattcaa ataatttacg gctgcgttaa agcccgtatt ggtgctcaca tcaaatgttt
    31741 ggccagcgcc ggcatccgta gaaaacggca cgatatgaac cttgatctcg ccgcctttat
    31801 aatcattgaa atcgccgagc aaattggcaa ctgccgcttt caaaagagtc agcctctcac
    31861 cagccattga acccgaaata tccagaacca gaacaacgtt aaagtcctta ttgatcgcct
    31921 gcaggctaaa ctcattgcaa tcgtcataag cgatcggccc gtcatctttc acgttaatcg
    31981 taatcgtgcc cgtatccgta tcaccgtcgc tatcgctggc cgttacacca aacaccagtt
    32041 gaatcgcatc atctggattc gtggcatccg gatgatccaa aacgcccagc agattaaacg
    32101 tataagatcc atccggctgg atggtcagcg taaacacgtc gcctgctgcc gtcgaaccga
    32161 cataaccggt ggtcgtttgt gtaattgtca ccggctgacc gtgtgaggtc aaaggcacgg
    32221 aagaattcgt tgaaccattc ggagccagaa cgcccggagc atccgtcccg aaatcaatat
    32281 ccagcttgcc gctcactgaa acgcccggca gattggtttc atcaaccgtc tcgatacctt
    32341 tgccaacttc cggcttgtca ttgatatccg gcacactgtc tgtaatgtga attgtaattg
    32401 ttgtgtccag cgcatcaccg tcataatcaa caacattgac gccaaaattc agatcaatat
    32461 tcgtgcctgc cgccgaatga tccaacggcg caaactgtgt aaattcatat gtgcccgtgg
    32521 tcgcattggt aatcaccaat ttaaacaccg ttgttgcgcc gccattaatt gttccaacat
    32581 aaccgtttgc cgttggtgta atcgtaattg cctgcccgcc ggaacttaga accccgccca
    32641 acaaagcacc cgtagcggaa gatgtgccgt tttgcaccat ttcacccggc gtatcattgc
    32701 cgtaatcgat attcagcgta tcgcccacgg taacaggccc gccggacagc gcattttcgt
    32761 taatgctctc atcatccgga tccgccataa ccggcgcatc atccaacacg gtaataataa
    32821 tttttgttgt ggccgtatca ccgtcgctat ccttcgcaat aacgccgaaa tcaagcgtta
    32881 cggcatcatt cggattcgtc gcatcggcat ggtcaaacgg cagaagctga ttataaatat
    32941 attcacccgt tgccggatta atcaccaatt caaacaccgt tgttgcgccg ccattaattg
    33001 tcccaacata accgtttgcc gttggtgtaa tcgtaattgc ctgcccgccg gaacttagaa
    33061 ccccgcccaa caaagaaccg gaaacggcaa aagcaccgtt aggaataatt acgcccggac
    33121 catcattgcc aaattcagca tcaattttac cgctttggga aagatttaag tttgtttcat
    33181 ccaaaatatg tttctgagga ccagccactt ccggttgatc gtcaggttct tcctcaagaa
    33241 acagcgtatc attctgaaac tcgatgccgt actgaagtgc cgtcgggtcg attggcccca
    33301 ccgccgccaa cggcccaacc ggggtaattt caggagaact gttaaaacca taaccggtat
    33361 tttgtatgcc accacccggc tgttcgccgg ctgccggttc aatttgcgca agctgctgcg
    33421 cactctgctc gccagacgca ggctcaacac tggctacttg cgtatctttg ccttcgccct
    33481 cagtagtctt ttcagtggag gaagcctctt tagcaacttc gcgtatttca gcctgaggct
    33541 cttgcaattc ttcttcaccg ggcgttgtat caaccacctt gatcaatctt ttaagctcat
    33601 cagcgctaag cgtattacca aaagtcaaag ttgcgggcaa aacgcttgaa gctgctacgc
    33661 caaatccgtt aagaataacc gcgctgccat cagcaaaacg cagcgtcaaa ttatcgccgt
    33721 cctgctcaaa ggactgcaca ttatttttat caaaatcgaa aaaatatttc tgtcctgcag
    33781 ccacatcaat ctgagtcgtt ttaccagcag cgggcttttc aataatgatt ggggctgtga
    33841 gcgtttccgt cgcatcttct gtggttacaa cgcacacacc gtcaaccaat gtgacattaa
    33901 cctgattttc ttcagtattt gtaactgttt cagacggttg gctggcatcg gcattctcat
    33961 ttgccatggt tctctcctta aatatacgta aaccaaaatc aggcttaacg ccctctcctc
    34021 aattggccgc tcataaacat gtacgagcta cttttaacag gttcttaaca gaatgaaata
    34081 atttttaacc tatcgttaag cctgctgtca acaattatgt taaaaacaag ccgtgtcttg
    34141 aataaatctt caactgccca aaaaataaat tttaagctta aaataccata tagcattcct
    34201 accaaatata cgttccccgc gatctacgcc ctcagctcgt acgaatcgct cgcacgaatc
    34261 actcgtgcga atcgctcgtg cgaatcgctc gtgcgaatca gggttttcaa acaaaaagcc
    34321 ttataaagtt ttacccgtga ttcgttaaaa ttggatgaaa tccaccatga ttttcgcttc
    34381 aaaaagcgct ttattaattc cgacatcaaa ttttataaat ctttggacaa gctgtggata
    34441 attttgtgga ttttctagat tctttgataa aaacaaaacg gttaataaac gtaataatat
    34501 taccattaat tctgctctgg agaaaataaa ttacaaaaat tgacaaaaaa gactcgacat
    34561 caatcaaaat agcgttaaat tgaaaatcaa gttcagacac tggtcgaaag acaaatgact
    34621 gatacgagag ccgtctagtc atcggttcgg gtcttcggaa acccgcactt ttgagaatct
    34681 tcggatttgc aagaggagcg ccggaggctt aaggagcaaa aaagcaaaag acactctttg
    34741 acaaccggac ttgtggtcgc gcacatgaaa acgtgagcag gttacctaag agattaaaag
    34801 tccaagcgtt tgtattttgc tctgaaatgt cagcacactc gcgagatgtg ctgatgagtg
    34861 gttgattgcc acgataaata aagtggatgg gggttcatcc acggatgcgc agaaaaaatg
    34921 taagtcgctc ttacaaactc tgcaaaatct aatctgggcg acacctgtgg ttattacaac
    34981 cgtacttcgt acctgtaata atgcttcagg atcatgcttc ccatcaggcc ccggtattcg
    35041 gtctttcatt atgacttcgg tcaaatggga tttggatgcc ggggttcttt gttatctcaa
    35101 ctcttgttac aattctaaaa ttcacgtaga actatttttg gaattaaaac gcaattcaac
    35161 accattgata taagcaataa acgaatatta acgggtttcg gataatttat atatatggct
    35221 ttccaatttg aaaaactcag tgtcctgatc gtagaagaca ctatccccat gctcaaactt
    35281 gtttcctctg tactcgacac acttggcgtc ggcaaaatat atacggcttc aaatggtgat
    35341 gaaggctata aaatatattg cgaacacaat cctgatattg ttgtcacaga ttggcacatg
    35401 agtcccattt caggtatcga gcttgttgat aaaatgcgca acaatagctc ctctccaaac
    35461 cgtatgattc cgattgtcat gatgacagga tttagcgcaa tgccgcgagt cgctcaagcg
    35521 cgcgataccg gcgtaaccga atttttggtc aagccgttct cggccaatga cctggcccgg
    35581 cgcattgcct acgtcatcaa ccgccctcgc gattttattg aaaccccgga ctatttcggg
    35641 cctgatcgtc gccgccgtaa aggagaaaat tataagggtc cttatcgccg tgccagtgac
    35701 ctgttagtac cctaatttgt tttcccggaa aaattaaacc tctggattat taaagggttt
    35761 tcacttaact tgcagatata tgaacaaaaa taaatacgaa gacagttacc aggaactaag
    35821 tttaccagca aatgtccaag acaaataatt ctgaaaataa accccgcatc tataaggcag
    35881 atacacacct tcagaataaa gttggcacag gcccgcttga tgaaaaggca attgaacgtt
    35941 gccaaaacgt catgaataac aacgacgttg attttgcccc tttagctatg gaattccttg
    36001 aaaagcttga aaatgcgata aaaagtgcca ttgccagaaa gaaatcgaaa gaagaatcca
    36061 tacaagaaat gacaaacgct gtcatgcagc tcaaagccaa cgcttcaacc ttccattata
    36121 ccctgatcgg caatatggct aacattatgc tgagctttct cgaaggtatc caggaaatag
    36181 acaatgacgc tattgcaatt gtcaatgcgc accaccaaac gctcaaagct attgttttga
    36241 aaaaaatgag tggtgatggt ggcgccattg gcaagcaaat ggaagacgag ctcaaagccg
    36301 cctgcaaacg atatttcgct aaacgcatgc gatagaaaag ccgcttacaa ggaaacggct
    36361 cttcacgaag ataaatgctt atggttctca gatcctatgg tatccgaagg aacggtccgc
    36421 cgttcgacag gcgaacgatt tcaccaagac gatcgagagt gcaccgtggt tttggaaaac
    36481 acgctcctgg gtaaggagca gaattggtaa agtcttccga aacctccgtg cggcatgaac
    36541 ggccgccgga accttctcta agagccgcaa tatgttgctc tgtacgatca aggattctat
    36601 tcattccacc aagttgctct ttgatttctt caaaggcggt atcttgtctg gcaagcatct
    36661 cgtccattct gacgcaaaga tcctgcaaga gccgctcaac aaccgccgcc gctcctgaaa
    36721 attcttcctg aagcggtttg gcatcgctct ttgcaccagt ttcgcttttg gacatctctt
    36781 taccccttgt catcaaacac caacgggccg tgtaagaaga aattattttt atgtcaacga
    36841 aatatccggt ctaaaacgac agccgcttgg cgctggtaat ttgcttttgc gccttgatct
    36901 tcgcaaacac ctcgtcattg accgggccgt ccagcgccac cagacaaatt gcgcccgaag
    36961 catcctcctt gcgtcccaag cggaaatcgg caatgttcag gccctcttgc cccagcagcg
    37021 tacccagctc accgatcatg cccggctggt catcgttgcg gatgaagatc atgtgcggtt
    37081 tgagcgccac ctcgatcggc acgccttcga tattgacgat gcgcggctcc ttgccggtaa
    37141 agagcgtgcc cgtcacattg cgcgtgcgtt cctcggatgt cacaatcacg ttaatcatgc
    37201 tgcgccagtt tttcgactcg tcgttatagc tttgcttgag ctcaatcccg cgggcgtgcg
    37261 ccacctgcat cgcattgacc atattcacgc tctcggtttg ctgccccagc aggctcgcca
    37321 gtaaggttga ggtaatcggg tcggtattga cctgcgtaat cgtgccttca tactcaatct
    37381 cgattttctt gatcgccgta ttgctgatct gcccggcaaa actgccaagc tgttcggcca
    37441 atttcagata gggtttcagc ttcggcgcat cttcagcaga aatcgaggcc atattgatcg
    37501 cattcattac cgcgccggta ttgagataat cgctcatctg ctcggccact tgcagcgcga
    37561 cattgacctg cgcttcggtc gtcgccgcgc ccagatgcgg cgtacacacc acattatcca
    37621 tgccaaacag cggattgttc gtcgccggtt ctttttcaaa cacgtccagc gccgcgccgg
    37681 ccactttccc ggattccagc gctactttca gatcggcttc attgacgatc ccgccgcgcg
    37741 cgcagttgat aatccgcacg ccgtctttca ttttcgcgat tgaatctttg ttgatcagat
    37801 tcgccgtctt gtcgtttttc ggcacatgaa tggtgataaa atcggcattt tccaacaatt
    37861 catccaactc gaccttcgta acgcccagat ccgccgcacg ctcttccgta agatacggat
    37921 caaacgcaat cacatgcatt ttaaggccaa cgccgcgggt ggccgcgatg ccgccaatat
    37981 ttccgcagcc gattacgccc agagttttgt tatacagctc tgtgcccatg aattttttct
    38041 tttcccactt tccggcatgc gtcgattcat tggcctgcgg gatctgccgc gccagcgcaa
    38101 acatcatcgc aatcgcgtgt tcagccgtgg ttatagagtt tccaaacggc gtgttcatca
    38161 ccacgacgcc gttttctgtg gcggtaggaa tatcgatatt gtccacgcca atacccgccc
    38221 ggccgatcac tttcagtttc ttgccagccc tgatcaattc gggcgtcgcc gttgtcgcgc
    38281 tgcgcaccgc cagcccgtca tattgatcga taatcgccag ctgttcctcg ggggaaaggc
    38341 cgggtttaaa atcgacttca ataccgtttt tttcgaagat ttcgacagcc agagggtcca
    38401 gcttgtcgct gataagaact ttcgccataa ttttaactcc taattttgtc ttgtttaggc
    38461 aaacaggcgt tcaggagtat cacgtttgga tgggaagtaa agagggattt tacaaaaatc
    38521 gtaaaccaca aaaccggttt tatattttca taatttcatt gacacggcag agccccgccc
    38581 tcataatccc gccatgctta aaggcacaga catcaaaaaa gggtttttaa gagcacgcta
    38641 taaggtggca acatctttgt taaacatcaa cgagcagcat gtcgttcggc tcgcaagtgc
    38701 catggcgcct ccaacggaag atattggcag atgggaagaa actgtggcca tacatgaagc
    38761 cgggcatgct gttactgcta tggcgctacc acatataagc gtttcctata tcaatatttt
    38821 gagagaaaca acggctgtaa actacggaaa cagtaaacaa gaattaggcg atgcaataat
    38881 cagtctttta gcaggtggtg aggcggtgaa aatgtcaaaa cgtacgcggc acatgacgac
    38941 gggcgacggt caagaccttc acctcgctga aatatacgcg cgcacccttc tcattgttga
    39001 agggcacaac caatcagccc ctgattttga gagatctgta agacggatgc ttgccacagc
    39061 aaaaatgaag gcatgggaaa ttttaatgca acactggccc gcagtagaag ccatcgctga
    39121 agttacgcgc atactcccta ttctagatca acgcaaattg caccatcttt tcgaggaaca
    39181 ccgacaacaa aaacttgagg gtatctcagc agattgctca gtctaaaata ctattctaag
    39241 cagcctcact ctggcgttgc ttttccatct tggctgctgc aaacgcccaa ccgatccaag
    39301 gcatcagagc ttcgacatca tcgcctgaga ccgttgctcc gcaccagatg cgcaggcctg
    39361 caggcgcgtc gcgatatccg gcaatatcat aggcaacatg ttcttcctca agcaatttgc
    39421 acatcgcctt gataaacggc gttttctcat cctcgggcaa gccttgaaac catgcatcgg
    39481 tgattttcaa acataccgac gtcgccgaac ggctggccgg atcttcggcc agaaattcaa
    39541 atccgtcggt tttagccacc cagttttcaa tcgccttgag gttttcttgc gcccgcgcga
    39601 ttgtcgtgcg aagcccgccg atattttcca cccaacgcag tgcatccaaa gcatcggcca
    39661 ctgccagcat ggacggcgtg ttgatcgttt cgccctgaaa aatcccgccg atcagtgcgc
    39721 cttttttggt caggcgaaag actttcggca acggccaggc tggcgtatag ctctccagcc
    39781 gctccaccgc gcgcggggac agcaccagca tcccgtgcgc cgcttccccg ccgagcactt
    39841 tctgccagct ccatgttgtc acatccagct tgtcccatgg cagatcatag gcaaacaccg
    39901 ccgaagttgc atcgcagaag gtcagccctt cgcggcttgc ggaaatccag tctccattcg
    39961 gcacccgtac acccgaagtc gtcccgttcc acacaaacac gcaatcattg gccggatcgg
    40021 tctgcgataa atccggtagt tgcccgtatt ccgcttcgta ggcgtttaca ttttcaagtt
    40081 tcaattgctt ggtaatatcg ctcagccacc cggatgaaaa actctcccac gcaaacacat
    40141 caatcccgcg cggccccagc aaagaccaca gcgccatttc gaacgcgccg gtatccgaac
    40201 ccggtacaat ccccaccaca taatcttgcg gaatgcccag caattccttt tgcttttcga
    40261 tcacatcctt gagctgcttt ttaagcggtc ccgcacgatg cgaacgtcct aggagcgcat
    40321 gactaagatt gtcaaagctc catcccggcc gcttggcgca cgggccggaa gaaaaacagg
    40381 gatttttagg tttgagggct ggcttttcca ttatgtctct catttttaac ttgtcattct
    40441 aagcgaagtc gaggaatctt agccttggaa taacgaacaa agatccttcg acttcgtgct
    40501 aacgcacttc gctcaggatg acaagcagga aataacgcat gagtacagtt ttaacccttg
    40561 tcgcctctga ccccaggcgc cccttgtgtg acggacatac gcgcgaagta atcaaaatct
    40621 taggcgctta taacgtccag cccacctgcc agcccatctg gatcaaaccg aaaaaggttg
    40681 ctgaaatcgg cgtgagcgac aacacatcca gcgcgctgtt catgcatttg cgcgattttt
    40741 tagaaaaaga cgccatcgat gttttcatca ctcccatcga aaaccgccgc aaaaagctgg
    40801 tcctggccga tatggattcc accattgtcc gcggtgaaac acttgatgat ctggccgaac
    40861 atgctggcat taaagaccag attgccgaaa ttactgcccg cgccatgaac ggtgagctgg
    40921 acttccacgc ggcactcaat gaacgcgttg gcttgcttaa ggggctggga atcgaagctc
    40981 tggacaaaac cttaagcacc atgatcatca atcccggtgc ggaaaacttt attcggacga
    41041 tgcataagca cggcgctcat tgcgtactgg tttctggcgg tttcactttt ttcaccagcg
    41101 ccgtcgcaaa gcaggtcggc tttcaggctc atcacggcaa cgtattggaa atcgaagacg
    41161 gcgcacttac cggcaaggtc aaaccgccga ttttagacaa acacgccaaa gtcgaatgct
    41221 tgcgtaccta tatggatcgt ttcaacctca aacctaaaga ttgcctcacc attggcgatg
    41281 gcgccaacga catccccatg ttaaaaacgg caggccttgg catcggttat aagccaaaac
    41341 ccgccgtagc aaaccaaatt gacaatctca tcatacatgg tgatctcagc gctgcactct
    41401 atgcgcaagg cttcactgac agcgattttt atttaacata aacaacaaac caagtcttaa
    41461 ccctctaccg ttattatagg gtttgcgcta taatggcgtt gtgcgtgctt gccgtgcgta
    41521 cattttttga gtttctcttg taacttcaac aaacacccag agcaaatatc atgtctgagc
    41581 aatggtcata tagcgttaca aacaacgaac tggaagagtt gttccggctg tattgtaccg
    41641 acttcacaac cctggcaaaa caaggacgtt acgggccgat taccggacgg gacaaggaag
    41701 tggatgaaac catccttatc ctgatgcaaa aaggtcgtaa aaacgctgtc ttgctcgccc
    41761 cggccggggt cggtaaaacc gcaatggttg tcgctctggc tcaaaaaatt gctgctgatg
    41821 acgttccgga ttatctgctg gacgcgcgcg tcatagaaat cgacatggct cgtatggccg
    41881 ccggaaccaa cagcccggca gaatttcagg aacgttttat cccgatttgt aaaggcgtcg
    41941 cggagcgtta tcataaccca gaaaatccgc gtatcattct atttatggat gaaattcacc
    42001 agattatgcc tggttgtgtc ggctcgtcct atgcgggctt gtcagatgtc atgaaacctt
    42061 acctaaccgc aggcgacctg atggtcatcg gcgcgacaac gcttgacgaa tttcgtatgt
    42121 atattgctga agacgccgca ctggagagac gttttcaaaa ggttttctta agtgttccaa
    42181 acactgcgga aacatatgcc attctcaaag ctctgcggcc aagtcatgag aaacaccacg
    42241 gcgttaccat ttcaaacaat aatctcatga caattgttca actgactgat gagcatatgc
    42301 gtaagcgtaa ccaaccggat aaatctatta tcaccatgga ttccgccatg gcctatcacg
    42361 ttaaaaccta tggcagaaac agtgaagtta cactcgattc aatttattat atgattggcc
    42421 gtgaaacagg cctgaacaaa caagcgctgc atgatgaaaa gaaaatccag cgtattatga
    42481 aacaggttcg cgaaattgaa ggtgaaggct acacttatta tatttaatct ctttcaggtt
    42541 taattcctcg ccgcttgcgg cgtaatcgtc atcgcgagcg cagcgaagcg atccagtgcc
    42601 acaaggataa attctggatt gcttcaccgc ctttggcggt tcgcaatgac atgatacctc
    42661 gccccttggg gcggggtgtt cattaaccaa gatttttgct gcgtttcctg cactattttt
    42721 gtgcgcaaaa aaccttaaaa ccctgtgctt caaatatctt ttcaaacgcc ttgaaatgct
    42781 gcttcaaaat agcttcatac ggcaaatgct tgttcgccac catccataat gcgcctccag
    42841 gcttcaggct gtgacacgca ttaacaataa aatcctcccc aattgtgatg tcggcagatt
    42901 ttccttcatg aaacggcgga ttcatcacaa taaaatccaa accccttaaa ctgtgttttg
    42961 tacagtcaca ccacaaaaat tccagcgctg ctgatatatc cggcctcgca agattttgac
    43021 gtgccatctc caaagcccgc caatctgcat ccagacatgc caatgatttt acagcgcaat
    43081 gcgcaagcac acggcgcgcc aaatatccgt aaccacatcc aaaatctgct cctttgccct
    43141 gtaaatcatt tggcaaatgc tgcgctaaaa gcgccgagcc ctggtcaact ttattccagc
    43201 cgtaaatgcc aggccaggag cgaaatgtgc catcaataac ctctttcggc tgcccggcgg
    43261 ccaatgcctt tttacgcgcc gccttatcaa cattgtcttt gtacgcccac acaacgcgcg
    43321 ctttatgacg tgattcactg caaatatcat acaaacccaa agtatccatg ttcttggcca
    43381 atcgtccgcc gccggccttg ttgcccgctg cgacaaccag tagaccgcca gacttaagaa
    43441 tctccagtgc gtacgccatc aaatagagtg tttcaatctg gtttttcggt gcggccaata
    43501 acgcaacatc atacgatgtc ccctcgtcag gaagaccggg aagcgctgta taaccttgcg
    43561 cctgtaatgc gctttcatac ggcttaaaat attgctgcaa ggttaagtcg cactgatcaa
    43621 attgccgcag tgctaacgtg acctgcgcat tcaaaaaaag ggttttctgc ccggccgatg
    43681 gccacggcaa agcaccggac tcaaatggat agaaaagtat ttgcagcgct gcgtcatcaa
    43741 gtaaagccat aggcccaacc atacgcgcaa gaacaatgaa aaaacaggag tttttctaaa
    43801 ctggaattga tgtctcggct tcaatgttac cgtctgcgat caaatccgcc agcgttgcat
    43861 tcaagccgcc atcaaaaacg gcaatggctt caaatgtgcc gccaatatca ccgtcagcgt
    43921 taatatgaag ctccgtatca ctgccatttt gtaccaattg tacaaaatcg cttaaggcat
    43981 catcgacagt actgtaacca accagaatat ctgcgatatt caaaatatct ccgccaacgc
    44041 cagcttcaaa atcaaagatt ttatcggcca acgcatccgg caataacatc acaaatgtat
    44101 cgctccctgt gccgccataa gcctgatcaa aaccttctcc gataaagagg acatcatcgc
    44161 caccctcacc ataaaggata tcgtttccaa gaccgccaat gagcgtatca gagccttgtt
    44221 cactggcatc tacacgaata cggtcaatat ccagatcaaa cagcacgtct tcataatccg
    44281 catcgcccaa attcggcaga tcttcaaaac ctatgcgcag cacattctga ttggccagat
    44341 ccagcaaacc ggatactgcg tgtgtatcgc cgtcccagtt aatgtccgga gaatctccgc
    44401 gcggcgttgt gtgataatga taaccgttta gaacctgctc ggttgtgcca tcattataaa
    44461 caaccgaaac cagattcccg tcatcattaa ttgtcgcagc gcgttcattc acgccaccgt
    44521 aatcatagac aaagcttatg acattatcac cggtaatatc caggccaaga tactcactat
    44581 taacccgatc accgtcggca ataataaaga atccaaactg accaccagct tcgccaactg
    44641 gtaattcaat ctggtgatct atgttgattc ccgcgtcctt cacattcgcc cacagcacag
    44701 atgccatttc gatcgtgccg tctgccgtaa tgctatatat acccagcgta ttattatatc
    44761 cggcaaaacc atcacggaat gtcaatgttg ccgaagcatc ataactgacc gtcaggttat
    44821 cgctgttaat ccccaacgcc ggggtacctg gcgggataag actttgaata ttcgtgcctt
    44881 cgatcaactc tggaaacgta atattgtcga tgaagtcctt atccagatcc atcaacccca
    44941 gatctgtgcc gccataaaga atgtcgtctc cggcgccacc gtcaagccgg tcatctcctt
    45001 gaccgccaaa aatctgatca ttatcgtcat ttccgttcaa aatgtcattt cctggcccgc
    45061 catcaataat atcatttcca tatgatccaa aaatctggtc attcccaata tttgcccaaa
    45121 gaatatcgtc tccggcgcca cccaaaataa ttacatcccc ataggtggta ttcgcatcag
    45181 ccagattgat cacatccccg ccgttacttg caaaaaactg ctcgatatta tgcacaacct
    45241 gcataccaac attgtctgta aggcttaaga aatcaccatc atcggtcatg aataaaaaat
    45301 ctgtaccgcc aagcccgtca tacgcgctcg aattgacacg aaattcttcg tcaacatcat
    45361 aagatttact actgtaagga ttaacaagtg tcactgtaat ttgttgaagc gttccagtaa
    45421 aaaaaattat atctgttccg tcagtcgcca tattatatcc c
//
LOCUS       DAOSWR010000003        39965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_650_length_39965_cov_5.433751, whole genome shotgun sequence.
ACCESSION   DAOSWR010000003 DAOSWR010000000
VERSION     DAOSWR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 39965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39965
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_39965_cov_5.433751"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK513_00395"
     CDS             <1..245
                     /locus_tag="PK513_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOO80947.1"
                     /translation="GKSGVRLETTDNLHMPPPGDVRIDDPQKQKRTYEVAPAGNAVIM
                     EEQLINSGQTVMDYNLMTNLYQKNTGMIYTALGRQR"
     gene            266..682
                     /gene="flgC"
                     /locus_tag="PK513_00400"
     CDS             266..682
                     /gene="flgC"
                     /locus_tag="PK513_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOO80948.1"
                     /translation="MGTELLNAMQISASGMRAQGTRIRVISENVANANTTGETPGADP
                     YRRQTISFKNVLDREAGVKKVEVNKIGEDYEDKFKLKYEPGHPAANEEGYVKYPNVEP
                     LIETMDIREAQRSYEANLGMIEQSRGMIMRTIDLLR"
     gene            720..1061
                     /gene="fliE"
                     /locus_tag="PK513_00405"
     CDS             720..1061
                     /gene="fliE"
                     /locus_tag="PK513_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOO80949.1"
                     /translation="MVDKIIDPNIAMNAYSRSMNIAQGAKGADETDSSGESGGAGGVT
                     FSDFLKQKASESVDTMRKSEEMSARAVTGEADLTDVVQAVTAAELTLQTVVSVRDRMI
                     SAYQEIMRMPI"
     gene            1106..1372
                     /gene="fliQ"
                     /locus_tag="PK513_00410"
     CDS             1106..1372
                     /gene="fliQ"
                     /locus_tag="PK513_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOO80950.1"
                     /translation="MNDEQILETVQQALLIAIQIGAPVLLVGLVVGVTIALVQALTQI
                     QEITLVFVPKILAIFLAMFIFLPSMMKTLSVFMESLADKMIGLG"
     gene            1406..1708
                     /locus_tag="PK513_00415"
                     /pseudo
     CDS             1406..1708
                     /locus_tag="PK513_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            1757..2536
                     /gene="fliR"
                     /locus_tag="PK513_00420"
     CDS             1757..2536
                     /gene="fliR"
                     /locus_tag="PK513_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOO80951.1"
                     /translation="MESAIETFLTTSVLAFMLTFVRLGSAIMIMPGVGDSFVSTRIRL
                     HIGVALSFALFPLAMPYMPDPLPSTFGLLVLIAMEFVIGLFIGTIARVLMSAMDTAGM
                     VISISSGLGNAQVFNPSMATQGSLVGALLSVTGVVMLFQFNLHHLLFIGIMESYEMFP
                     LGAIPDTGSMAELMSRTVSASFSIGVKIAAPFIVMTLLIYVGMGVLSRLMPQIQVFIL
                     ALPMQILLSIALLAMVLSAAVMYWIAQFEQGMVFFLRASGG"
     gene            2745..3821
                     /gene="flhB"
                     /locus_tag="PK513_00425"
     CDS             2745..3821
                     /gene="flhB"
                     /locus_tag="PK513_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOO80952.1"
                     /translation="MSDNQEQDDAQKTEDPTPKKLEEARKKGQVAVSKEINNWLMLFA
                     GTILVALMAPFAFTNLLEILKAFIERSYAFPSAPGGMGVILGGGLKQVLMALALPLAF
                     LFIVAFLAPFVQVGPLFAPEAIKPEISKISPIKGFKRLFSMRSLMEFAKGLLKLAIIG
                     VVGVIIIYPYFDKFEHFIGLPIPLLMGELKSLVVSLMIGVLIVLVVVAVIDLVYQRHE
                     HAKKMRMTKQEVKDEYKQTEGDPHVKARLRQLRSEKARQRMMQAVPSADVVITNPTHY
                     SIALKYDPETMEAPLCVAKGIDEIALRIREVAKQHDIILYENRPLARTLYDTVEIDET
                     IPVEQYQAVAEVISFVFKQKGKLH"
     gene            complement(3827..5758)
                     /locus_tag="PK513_00430"
     CDS             complement(3827..5758)
                     /locus_tag="PK513_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80953.1"
                     /translation="MAASSYDNFTAFYAQDPSVFGNTYETSVKIATVRLLDSQNNDTL
                     NQKINAALHAIDTGQGDPESLINDLQAYVDDRFSAVGGPQLSPQEAVSYKHSHERGVQ
                     DLIKSQHPDVNAREILLSLGRYEDAMPEQDRAQLNNDTKAFTLFTNMLPADNSSQAKF
                     LEAYDHMSTARTNIEQKYQGYDELQVLSHKIGDTDRALTQNFGYQNTLFDVEGHEIVL
                     PTGGYASALLDKRPEVFDTSKPLFDHNAFDAFTQRARTLQQGVENAISAGPPYNNLED
                     LKTGYGTLRGEIRDFSQDAKQNFLNRAQQYYDEELALLKQVHADNPHASSFADVALYL
                     KDVGDHPDITGSAYGRSGYMIEASMNAVRNVRELIGKYDEPAIRKAFDTLKSDSVEVS
                     AAPAAPAAPTEPEPAKPASEPLVIHITGGTEMKLSGRMAIVGRGTQVVEPRTAPSEEV
                     RKMQSALVAAGFSVGTYPDGHPKAGQALTDGQEGDKTRGAALKAAEAMNLTEEQLRTM
                     PIEEFTQKLEDYKLKMEQKHGVVVPDSKSEGTQVAGAEAPLACPVEGGRSPYALDQIL
                     KQSPVGEIRELGRGTPQMCFTDEGQNMLIGGNPASENFQNFAVANPAVVEPPQSSPEL
                     KPDQEAPAVQNDTVTLRLA"
     gene            5861..8410
                     /locus_tag="PK513_00435"
     CDS             5861..8410
                     /locus_tag="PK513_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO80954.1"
                     /translation="MSLDHSEFIRRHHKTAQTQSPPGPVRRRHDGLFAVILFTIFAVF
                     LGYILTLAPLSQDMKIITSMALALCCLVGFILYRGYHNHRQLDEELELVREVMEGSRG
                     ARLIIDSADNTLYYNQKFERLCRGVGPPSFSSLLRLFDYSDEVAAHFRLLTDQAHRGL
                     TDSIELFSRYEDQERWLLITAQPIAGRAGYVHWRIDDVTEKHTADTAIRAEREKLIDF
                     TDNAPVGFFSVDEKGRFVFANATFARWLGEDLQILLESGRLHTYMLNPPEDANPYDLI
                     KEGTARQITEVQMKGTAGKTFLASINQVIVREGAGPDGKQRVRTRAVVHDLTTESEMR
                     QALKASEDRFQRFFDEAPVGIILIDEEGKVADCNAAFAGIIETPIDKIEGQTFNDLID
                     EEDRDEVTGALGRIEKGQRLDAPLEISLKGATGPVETQMHAKKFKLDGRTVLHFIDLT
                     EKKALEVQFVQSQKMQAIGQLAGGVAHDFNNLLTAIIGFCDLLLLRHKPGDPSFGDIM
                     QIKQNSNRAANLVRQLLAFSRQQTLRPRIHDVSDILYELSHLIRRLIGTNIELELIHG
                     EALGPVKVDEGQMEQVLINLAVNARDAMDEHAREGATLTIETQNFTNRKTKKLISEEL
                     PPGHWVTIKVSDTGCGIEKKNLSRIFEPFFTTKQVGEGTGLGLATVYGIIRQTGGFLD
                     VESKVATKDKEGGTSFIIYLPRISKDEAEAEEEHPETEEVEAKDLTGTERILLVEDED
                     AVRTFSERALTNKGYEVLTADSGESALNLMEKQENQKIDLLVTDVVMPNMDGPTLAQR
                     MRQTSPGLKIIFMSGYTEDKLKDHMGENIFFLPKPFTLKQLAAKVKDVLGD"
     gene            8583..9710
                     /gene="recA"
                     /locus_tag="PK513_00440"
     CDS             8583..9710
                     /gene="recA"
                     /locus_tag="PK513_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOO80955.1"
                     /translation="MSVTPLRKDDEMTQNSAEKQKALDAALSQIERAFGKGSIMKLNG
                     EQNTMNVEAISTGSLGLDIGLGIGGLPRGRIIEVYGPESSGKTTLALHVIAEAQKAGG
                     TCAIVDAEHALDPGYAKKLGVKVDELLISQPDAGEQALEITDTLVRSGALDVLVVDSV
                     AALVPRAELEGEMGDAHVGLQARLMSQALRKLTGTIARSRTIVIFINQIRMKIGVMFG
                     SPETTTGGNALKFYSSVRLDIRRIGQIKDKDEVVGNQTRVKVVKNKMAPPFRQVEFDI
                     MYGEGISKMGELLDLGVAGNAVEKSGAWYSYDGQRIGQGRENAKTFLRENPKMAEKLE
                     KQIRANAGLIAEDMLTGPDNDEGPELTAIEPETHPDDKKKA"
     gene            complement(9825..10313)
                     /gene="smpB"
                     /locus_tag="PK513_00445"
     CDS             complement(9825..10313)
                     /gene="smpB"
                     /locus_tag="PK513_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOO80956.1"
                     /translation="MSKKKKSGLLSTNATVADNKRARFDYALEDKFEAGMMLHGTEVK
                     SLRHGQANIKEAYVGPKNGEIWLFNANIPEYQQASQALQHEPKRPRKLLLHKREVDKL
                     LGAVSREGYAIVPLRLYFNARGMAKIEIALAKGKRQYDKRETEKKRDWDKQKGRIMRE
                     KG"
     gene            complement(10350..10664)
                     /locus_tag="PK513_00450"
     CDS             complement(10350..10664)
                     /locus_tag="PK513_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80957.1"
                     /translation="MSLSDDDRKWIEKTIAEKFAEFSPPPKKKKRKKKSPFGNTFKKH
                     SASLYGGGVEPFYGHRRPHNLQNMSFHQIEDHVLSLGIRSQIPEDMRFSWDEIDGNNY
                     KL"
     gene            complement(10729..11625)
                     /gene="dapA"
                     /locus_tag="PK513_00455"
     CDS             complement(10729..11625)
                     /gene="dapA"
                     /locus_tag="PK513_00455"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO80958.1"
                     /translation="MFRGSITALVTPFKDGKIDWKAFDNLVEWQIEQGSHGLVPCGTT
                     GESPTLSHDEHMAIVKRCVDVVKGRIPVIAGTGSNSTSEAIELSVAAKDAGADAHLSV
                     VPYYNKPSQAGMIAHFTAIADAAALPMILYNIPGRSVVDMGNETIATLAKHKHIVGVK
                     DATGNVARVAELVAMAGKDFCQLSGNDDCVAGFLAQGGHGVISVVSNVAPRMHADMVE
                     AWEAGDLKTFAALRDKLAPLARDLFCESSPAPVKYAAQRLGLCRDELRLPLIPASEAA
                     RAKVDAALAHAGLSVASKASAA"
     gene            11638..13677
                     /locus_tag="PK513_00460"
     CDS             11638..13677
                     /locus_tag="PK513_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOO80959.1"
                     /translation="MFHQSKSVHNLSALTRRCKKISALAVIFSIFITASAFPKDSPIN
                     RLQSARAQKQTIRALQLVHQGQWNLAHDAFAETHDPLASKLYYWLIFSQKGDFENYVR
                     LTQFIRNNPEWPGIDELRRRAEEKMPENLRPEEILAWFTDYKPSTARGVDRYMFAMIT
                     TGKTIDAKSFLADWWASTTLSRDDQRMIFRKYNTYLDRAAHLRRFDTMLYRGQNVNAR
                     ALAGVLGQGYPQLAEARIALRAEKKSVSHLINAVPRSLQGDAGLLYERLRWRRKNNLD
                     MRAAEILHRPPPIEKIQNPKDWWTERHILIRRMIEKRQYRSAALLASKHIQKPGSFEY
                     AQGEWMAGWLTLRFQDNPTQAYQHFEALYQNVQTPVSKSRGAYWAGQAAAKFTDPSIS
                     KDWYEKAARHQTTFYGQLAGAKLGQDGAFPGAAPPKLDDAELADFQRTELIQAAQLFA
                     RAGMQEQSSRFLWAFVKHEGSAKAYKYAAELAIKNGQTHEAVRISKEATKKGLFLTAQ
                     SYPVITHQLRDIDLEWALIHALIRQESMFDVKAQSPAGARGLMQLMPATAEETARKMG
                     IAHNTVWLTSNPQHNIRLGAQYLFDMIRRYDGAYPLAIAAYNAGPGRVDQWLKTFGDP
                     RTREVDWIDWIEMIPIYETRNYVQRVMESVYVYRLRLQKVQRPSEAPLHIALTQQ"
     gene            13845..14537
                     /locus_tag="PK513_00465"
     CDS             13845..14537
                     /locus_tag="PK513_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO80960.1"
                     /translation="MKLLLADDHTLFRDALVQYIERSDPDAEVSVAKDFHGAYEHMQK
                     DGKQDLILLDLRMPGMDGLQGFEKMRKAFPETPVALMSGIAEPEDVRAAMDLGAVGYF
                     PKTLSGKALLQAIGRVLEGEIFIPTDHKTQQLMPAYYNDIPLSAPACENNVPPQAKQP
                     EKNFKLTPREQEVLKHLTTGASNKEIANHLGLKVVTVKLHVRGICRKLEAKNRTQAAL
                     IARENGLALGAG"
     gene            14555..14881
                     /locus_tag="PK513_00470"
     CDS             14555..14881
                     /locus_tag="PK513_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80961.1"
                     /translation="MLNFFRRYYHLFLFIPGLIILGVGMLTLHTSIKLSSEQNLLRQH
                     YQMILTQHPGFSDSAAGRKIKILTKGVQTQEKNLYWSVIAIALTGFLLLIINADHLRK
                     LVEANK"
     gene            14915..16546
                     /locus_tag="PK513_00475"
     CDS             14915..16546
                     /locus_tag="PK513_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO80962.1"
                     /translation="MAAIEASFEGIAIVDPQGLLTYMNKALADIYGVAEEQKEDYIGH
                     FWLKLYSDENQAYIKNHVLPQFEREGFWHDTSKLLRRDGRAITAELSMTRLSDGGFIG
                     TARDVTEIEKASAEKKEIQNQLAQAQKMEAIGRLAGGIAHDFNNILAAINGYAEFLTE
                     DLDEDSPQKKYADNILKAGYQARALIDQILAFSRRQDGDVENIDLCGPLNESISMLKA
                     TLPKKIAVHTDIQVMPAPIEGNATQIAQAIMNLCVNARDAMRDDEGVLSISLKTVDVM
                     NDIPMTSVSDDLPAMEDTPLISIEEGEQGQTYLHFGTVSRNHQYIKLSVSDTGTGMSR
                     MIMEHIFEPFFTTKDVHKGTGLGLASVHGTMTSHRGALFIESVLGKGTKFDLLFPLSK
                     VDEQSETDIDMHELETHGGLVLLVEDQDEVREMTSTMLKRLGYDVETARNGLEASDML
                     CEKPGEYDLVLTDQNMPKMTGTELIRQSSFDFPELPFVILTGYSEEKLLEFMQEQPAL
                     KDVLRKPVSQKILAKTLSEVLKGTANSPTKKAAAI"
     gene            complement(16543..18645)
                     /locus_tag="PK513_00480"
     CDS             complement(16543..18645)
                     /locus_tag="PK513_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80963.1"
                     /translation="MNNLSAQSLSLHFTPLIPEAMLCTFGLLALVLVLAKIFTAGRFP
                     IFRALCFAFFLFILAGPSVHTEKREAVPSVVAVVLDESASQDFGQRKVRSLDALEYLQ
                     NTIERNESLEMRLVKGPHDGALASSTNLFESLETALADVPEGRRAGVIIISDGQVHDI
                     PDVEKLQESYGPVHLLLTGEKNETDRRIVVTNAPAFGIVGQDIDVRFRIEDSGALHET
                     HVQVSLQMSDGTVRRDVARVGEEHTMSLPVHHAGQNVFELFVEPLPGELTERNNRAAI
                     DFQGVRDRLRVLLVSGKPHAGGRTWRDLLTGDPSVDLVHFTILREPDKVDATPQNEMS
                     LIAFPFRELFELKLYDFDLIIFDRYRLNRILPDHYFRNIARYVEKGGAFLEASGPAYA
                     SEDSIYYTALRDILPGVPNGQVYEQAYTPKITTLGQGHPVTRSLLWSGGGSMRKDKKT
                     PWGPWLRQIGLQNVKGHVLMHGIDERPLLILNHVGKGRAAQIASDHIWLWSRGYEGGG
                     PHGELLRRVVHWLMKEPELDEQILDVRVDGHTLILRRAAFERSKDVVKMRAPDGREEE
                     LALTPTQNGLLEHFHRAKQNGIYRFETADGGLRFAVVGEVDPPEFRNVLSSAELLQPL
                     LEHSRGKALWLKDTPRPRLRMMRHGPYGGQDWIGLQDTQAFNVIGAEEKPAITLWLSL
                     TLILALMLGTWFYEGRRR"
     gene            complement(18642..21377)
                     /locus_tag="PK513_00485"
     CDS             complement(18642..21377)
                     /locus_tag="PK513_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOO80964.1"
                     /translation="MIGGLFGSLAFLNPWALTALLALPVVYILLRITPPVPKTVAFPA
                     VRLLDGLEPKEHTPSHTPWWILLLRLLMAALLLLALARPVLNPSQSAGTNGPLLLMID
                     NGWGAAQNWGDIQSAAISEIEQAGRQKQNITILTSAPEADTKAPQVYGPVTVSDALSF
                     VRALKPYPWPAALGALRGILPDLSDAHVLWLSDGLEKPGQPALIRALAAQSKNGKVIF
                     HVPAQGSLPLSLKSPEKFHSQPSIMIDGPHNLEPNAPVRVQLLSAQGKILDDKLVETD
                     GQSFPVSVSFDLPQAQRNQASAFRITGQNTAAAHYFMDASGGPKTVAIIAPEEAAQAS
                     QLTEDSFYLIKALEPYTTLLQGPLGEIIDQDPSMIVLPDIGVLPPETLNGLSAWVEAG
                     GLLLRFAGPTMTTQARGQDTLVPTPLRSEARNVKSSLSWDKPLAATPIAPDSPLYGLD
                     ADEDLRVSGQILPEFSSDLEDKTWMQLEDGTPLITAAQRGGGLIVMVHTTASPEWSNL
                     PLSGFYVQLLKHLLKFSGHSGALSLDQSGMLQPVQMLDGFGSLQSPAAHVLPIDAADF
                     EKQTPMPQHPPGFYGRGGLQKALNLGDHLPPLRASLGGDGMVLKTYDRAFEKDLAPLL
                     LSAAFVLFLLDALIMLFLSSAKWPRLSFKGKTVAVLILLAFSAPARAQIPQESQDLTL
                     AYVRTGNVSIDALSEKGLKVLAVALNMRTSAEPKHVRGVSLETDTLVFYPLLYWPISA
                     AQEPPGAEALQNLQAYLDHGGSILVDTRDGQKSSPALRKVLSGLNIPPLSPLPEDHVL
                     NKTFYLLKSYPGRYEGAPLWVEGQSAGGRDGVSSVILGSHDWVGAWAELNIVTRGGQQ
                     YLSGMTPRHEQSLRFGVNLVMYALTGNYKADQVHVPHILERLGRGSPARNGRSHP"
     gene            complement(21374..22228)
                     /locus_tag="PK513_00490"
     CDS             complement(21374..22228)
                     /locus_tag="PK513_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO80965.1"
                     /translation="MRLLPLRSEAERAAAQIPDLRALAVKTAFHVLHGANPRKRPGAG
                     EAFWQFREYQPGDMPCAIDWRQSAKGDRIFIRQKEQHNAQSCLFWTKRNADMNFKSDT
                     CRFDKKHVAGVLALTLALLHSRGGEMIGFAGTQRPGHSEKTLSSFEHILMRTDPCALP
                     RSISIPRHAHFYALSDFLEPIANIRAALKPLAECTRNGWMVVILDPAELALPYEGRIH
                     FEDMAGERRVMIDNARDIREAYQTRMEAHLQDLQALAAGWGWKFIVHSSDRLLEETVL
                     QIWLENAQ"
     gene            complement(22233..23231)
                     /locus_tag="PK513_00495"
     CDS             complement(22233..23231)
                     /locus_tag="PK513_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO80966.1"
                     /translation="MTKAAPKPEEYFDEAAAKLQAAKTEIEKIVIGQSDVVANTLITL
                     ASGGHVLLIGVPGLAKTLLVDTAATALGLDSNRVQCTPDLMPADIVGSEVLEDDNSKE
                     GGGSRAFRFIKGPVFTQLLMADEINRASPRTQSALLQAMQERSVSAGGETHMLPEPFH
                     VLATQNPIEQEGTYPLPEAQLDRFLMQVDVEYPDEQAERDIILATTGTDQASAAQAMS
                     AQDLLNIQTLVRAIPIGEQVVEAILKLVRSARPGESSPELVNEYVSWAPGPRASQALM
                     LATRARALLDGRSAPSIDDVIALAVPVLQHRMALNYKAHSEALDEAYIIRNLSENL"
     gene            23310..24494
                     /locus_tag="PK513_00500"
     CDS             23310..24494
                     /locus_tag="PK513_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO80967.1"
                     /translation="MDQKPVSMLEPQDWMKADETQSVMKALGQGYALFVGGCVRNALL
                     GQSVDDIDIASALTPDEVTKKLEAAGIKVIPTGIDHGTVTAVTGGKPFEITTLRKDVE
                     TDGRRAVVEFSTDWAEDARRRDFTMNTLLMDLEGNIYDPLGCGLKDLHARRVVFVGDP
                     RKRIAEDYLRILRYFRFHALYADGAPDAQALAACRTAADKIAALSKERITQEFFKILS
                     VDDPLTVLILMFENDILKEFKTPEYDERLLKSLCYFQKNYGLVSIAARLLILAGFAEK
                     NLKAFDDFLLIPKVFKKDIENLNQVLALPDLKDDHAVKVAIYKHGRTATAQGLMIELA
                     LDRVMNGYAPQAVKIIQNWDIPDFLLTGEDLIKEGFKPGPQLGEELQRREDAWIKGGF
                     KN"
     gene            complement(24508..24696)
                     /locus_tag="PK513_00505"
     CDS             complement(24508..24696)
                     /locus_tag="PK513_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80968.1"
                     /translation="MSSKFEAASKKLNEDRKTATEPLVLKPVDGTHLADQDGTYYRVV
                     EASHNGVALEEAPGVWNK"
     gene            complement(24823..25722)
                     /gene="hemF"
                     /locus_tag="PK513_00510"
     CDS             complement(24823..25722)
                     /gene="hemF"
                     /locus_tag="PK513_00510"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HOO80969.1"
                     /translation="MNDWDERKAQASVWFRRLRDNICAEFETIEGELSGSNKADLAPG
                     AFERSPWARENNDEPNESGSILKGGGEMSIMRGRVFEKVGVNISEVYGVFSEEFAARV
                     PGANKSGGQFWASGISLVAHMQSPLVPAVHMNTRMIVTSKGWFGGGADLTPMFPNEDD
                     TRAFHGALQECCDRHDPNYYKAHKEWCDRYFFLPHRGEPRGVGGIFYDYLDSSDTGGD
                     WTADFAYTQDVGRTFLAIYPQIVRTHMNEGWSAEERQFQLIKRGRYVEYNLLYDRGTQ
                     FGLKTGGNTEAILMSMPPEVKWP"
     gene            complement(25719..26174)
                     /locus_tag="PK513_00515"
     CDS             complement(25719..26174)
                     /locus_tag="PK513_00515"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOO80970.1"
                     /translation="MLSIALYQPDIPQNVGAMIRLCACMNVGLEVIEPCSFPWDVRKI
                     RQSAMDYIDHVNIVRHTSWDAFTGAHEGRRVVLMSTKAAVPYVDFEFRDDDILLAGSE
                     SAGVPQEVHESVDARVLIPMHGVMRSLNIVNASSMILGESLRQTRWSAV"
     gene            26320..26844
                     /gene="petA"
                     /locus_tag="PK513_00520"
     CDS             26320..26844
                     /gene="petA"
                     /locus_tag="PK513_00520"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOO80971.1"
                     /translation="MTADSKDRRDFLQLTGAAFGAVGIACAAAPMINSLNPAKDTLAL
                     ATTEVDISGIPVGGSKTVMWQGKPVFIKHRTAEQIEEAGEVDISTLIDKQTDEQRVKK
                     PEWLVVVGVCTHLGCIPGGQKPTDAHGEYHGWFCPCHGSHYDTSGRIRKGPAPKNLMV
                     PPYEFISDTVIKIG"
     gene            26872..28158
                     /locus_tag="PK513_00525"
     CDS             26872..28158
                     /locus_tag="PK513_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HOO80972.1"
                     /translation="MGSTERAEFKNPLIGWIDERLPVFTLMQKEYGVFPTPRNFNYFW
                     NFGAIAMFMLITMIVTGIVLAMHYTANTGLSFDSVERIMRDVNGGWLIRYIHMNGASF
                     FFVAVYIHIFRGMYYGSYKRPRELLWILGVAIFLLMMATAFIGYVLPWGQMSLWGATV
                     ITNLFSAIPLIGDSIVTLLWGGFSVDNPTLTRFFALHYLLPFVIFAVVFLHVWALHVT
                     GSNNPLGLEPQSEKDTLPFHPYYTMKDSFGLVVFLVIYCVFIFYMPNALGHPDNYTPA
                     NPLVTPPHIVPEWYFLPFYAILRAITFEIGIPFTEITIISAKLGGVIAMFGSILLWFV
                     MPWLDSHPIRSARFRPLYRLFLLLLLVDFVFLGYVGSQAADAEIFHIPLSYFGLGATG
                     YYFAFFIVILPWLSKFEKGRELPPSIHEAVLAKHAK"
     gene            28175..28936
                     /locus_tag="PK513_00530"
     CDS             28175..28936
                     /locus_tag="PK513_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HOO80973.1"
                     /translation="MYKKSLLILFVLLVLPVSGAFASGGETVHPPSQSWSFDGPFGTF
                     DRAAMQRGLKVYREVCSACHSLKRVAFRNLTDLGYSEDQVKAIAAEYSVMDVPDEEGE
                     VNERTALPSDRFPSPYPNDNAAKAANNALPPDLSLITKARHDGPNYVYAILTGYEEAP
                     AEVELLDGQHYNEYMSGHVIAMAPPLSDGAVQYEDGSLQTTGQYAHDVVQFLTWAAEP
                     SLEARKRTGVKVLLFLLVFASIFYGIKKKIWASVH"
     gene            29047..29394
                     /locus_tag="PK513_00535"
     CDS             29047..29394
                     /locus_tag="PK513_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80974.1"
                     /translation="MSTNAQKTAQIDKECADKTCAKEKLIRAKFYRFMAIGCMLSAFV
                     ISGYIFEILGHGNPAYIVHNPLVVLLLLLPFLPAVFMAILSKSRRAQAIKILQPYYDK
                     KNASDVRCLKESE"
     gene            29421..29972
                     /locus_tag="PK513_00540"
     CDS             29421..29972
                     /locus_tag="PK513_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HOO80975.1"
                     /translation="MRDTQQEIIHFWFEELEPQQWFQSSAAVDAEILERFALTHEMAD
                     DGLCNHWAVDAEGSLALVIVLDQFPRHMYRGQPKSFASDERALLVAKQAVHKGFDQIL
                     EPVKRGFLYLPFQHSESLSDQIKSVELFGAMAEDNPAGDMYARRHYVPIEKFGRFPHR
                     NKILGRESTAEEIEFLKTHKGFL"
     gene            29969..31474
                     /locus_tag="PK513_00545"
     CDS             29969..31474
                     /locus_tag="PK513_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO80976.1"
                     /translation="MSKYENTLKALGVRTDKAGSLEVFSPIDDSSLGKVKEHSSSDIE
                     AIARNAQSAFEDWRKVPAPVRGEFVRLLGEELRTAKDDLGKLTTIECGKILSEGLGEV
                     QEMIDICDFAVGLSRQLYGLTIGSERPEHHMREVWQPLGPVGIISAFNFPVAVWCWNA
                     AIALVCGNSIVWKPSEKTPLTALACQKLFDKAAAKFKVSGHDIPDHLSQVLIGGAQVG
                     QAMVDHPAFSLISATGSVPMGRAVGQAVAKRLGRSLLELGGNNALVVAPSADLDMAVH
                     AILFGAVGTAGQRCTTTRRLIVHEDIYGSLIPALQKAYEQIRVGNPLKDGTLVGPLID
                     HGALDQMMEALEEAIEQGGKILAGGERAHSDEPGTYVHPALVEMPKQTKTVCEETFAP
                     ILYVMKYKTLDEAIALQNAVPQGLSSAIFTNDVREAERFLSATGSDCGIANVNIGTSG
                     AEIGGAFGGEKETGGGRESGSDSWKTYMRRQTQTVNYSDELPLAQGVRFDL"
     gene            complement(31746..32450)
                     /locus_tag="PK513_00550"
     CDS             complement(31746..32450)
                     /locus_tag="PK513_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HOO80977.1"
                     /translation="MATAVNMSGQLLMPKATAVWLIENTALTFRQIAEFTALTEIEIE
                     ALADGEIGRGLVGRNPMESHELTQEELERCQADPSANLKIAKSSLPPVKTRAKGPRYT
                     PVSKRGDKPDAIAYILKHNSEISDAQICKLVGTTKPTITAVRERTHPNTPNLRPRHPA
                     ELGLCTWQEYEKASDKGLKAQGKDPEEVKAQRLAEQQASLSQPEESDDSSSSGGFDFS
                     NFLGDAQKFSSSSEED"
     gene            32613..32795
                     /locus_tag="PK513_00555"
     CDS             32613..32795
                     /locus_tag="PK513_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 family protein"
                     /protein_id="HOO80978.1"
                     /translation="MFDDDLPKPKVAEFPRNLENMSVSEREEYIEELEAEITKVETDI
                     SKKKASHEAAASIFKS"
     gene            32811..33851
                     /locus_tag="PK513_00560"
     CDS             32811..33851
                     /locus_tag="PK513_00560"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005524127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HOO80979.1"
                     /translation="MRIEESTKLDFKDVLIRPKRSTLSSRKNVDIAREFTFKWSGKKF
                     NGVPIIAANMDGVGTFAMAKAFAEDGNGLSVALHKHYPLKELQKFYGDAGHGSVWYSI
                     GMVKEDEEKLDEFMKAGHHPDKICVDVANGYSEHFVDFVKSIRNKYPDLTIMAGNVVT
                     GEMVEELILAGVDVVKVGIGPGSVCTTRKMTGVGYPQLSAIIECADAAHGLGGLICGD
                     GGCTVPGDIAKALAAGADFVMLGGMLAGHDESEQKIVKEGGEKFLQFYGMSSSTAMKK
                     HKGGVAEYRASEGKTVKVPYRGPVEASLQDILGGLRSTCTYVGARQLKELSKRTTFIR
                     VSQQLNTIFGNE"
     gene            33932..34279
                     /locus_tag="PK513_00565"
     CDS             33932..34279
                     /locus_tag="PK513_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80980.1"
                     /translation="MSDEVKQRLQDSTANCLKAYDAWDGDKKNATARETLQDAVHELR
                     KVASRLEIEIAVSDRKQMTQKPMPIPAHRDARGAQKKDDADLSKGNEKKSDNPKPKLK
                     PKKDEKKLEGDEE"
     gene            complement(34283..35002)
                     /gene="gloB"
                     /locus_tag="PK513_00570"
     CDS             complement(34283..35002)
                     /gene="gloB"
                     /locus_tag="PK513_00570"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOO80981.1"
                     /translation="MINVTLIPVHKDNYCYLLEGDDGSVAIVDPGEAGPVIAALEAKG
                     LKPDVILITHHHWDHIDGTSGLLKWHPCPVAGPASETARIPQMDIFLDEDSNFKFGGE
                     ALHVLETPGHTRGAICLYFPENKLVFTGDTLFSMGCGRLFEGTPEQMWHSLQKIAALP
                     DDTQVYCGHEYTLANAEFCLRIAPDNAKLQERVKQVRALRKQGKSTLPVLLKTEKETN
                     VFLRAGSAKDFAKLRALKDQA"
     gene            complement(34999..35808)
                     /locus_tag="PK513_00575"
     CDS             complement(34999..35808)
                     /locus_tag="PK513_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO80982.1"
                     /translation="MVKPNDNTTLFDPDARRVARRRAASSFDQYRFLHDMAWGQILDR
                     LGDVRRDFGARHNLSAHDFDNETEALAFEPESLALITSCLNLHAVNDLPGLLVQIRKS
                     LKPDGLFMAAMFGGETLYELRESLMETELTLKGGASPRVFPFADKQQMGGLLQRAGFA
                     LPVVDSDIVRVSYEDIFKLMHDLRGMGESNVIAARSRINPGKAFFTRAGQYYQEHFTE
                     KDGRITASFEIIYLIGWAPHESQQQPLKPGSAERRLADALMTREEKAGEPL"
     gene            complement(35802..38435)
                     /locus_tag="PK513_00580"
     CDS             complement(35802..38435)
                     /locus_tag="PK513_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO80983.1"
                     /translation="MAEKLETEAESYIEKRNLLLKDIEPAVISKAWFTLSHLLLDEGA
                     HVVDMGCDDGAMTYAMAAMNPKVHFTGLDKAKRQVNKAKEIYKLFNLDFKVGDASSEI
                     FEPESVDAVINSYILHEIFSNSRYSEQTVSDTLRKQFKMLKKGGVMFIRDFAKPPPEE
                     FVLLEMPDEPSTGKTLARLSPPDILVWYAEHARPRQDAGCGGFFLEELPERFPRTRLF
                     RLPYKWAYEFIMRKDDRSHWESELPKEYTFFTPMEFRKELSALGARVQYSGPHWDDDL
                     IKEKFEGRFRLYADDGTPLGDPPTCYVAVAYKMAERKSLHIEERRPSSTDSGKLKITA
                     MRNEKTGEIMDVVSRDIDVSEVIPYRVDEEGRLKIYLHDGVARSITNAVPRGGVNIDE
                     RRWSGHMAEAITVDGEAISSIETFDVKHSVLFARDYLGLKPEANAIIEHGPDYYPAPD
                     YIDERIHTFYLRVCPSKNAVTPKKVIGVEKFQAKGVVREMDAQQVLNAITVGMIPNAR
                     LEMQILSLFHHLRLRAETWTSRQLNLQVSKINKKADVKNLLTKMGEEDFRFKKVKGTT
                     GQLRSVHSTFVEEGQSRGSISGLSAESVDFIVHDGKTINTAVVLPLTKDEKNTVHAGV
                     VTHHLPVPQRHEGNGLTISVPSFNVPAEVTNLKMMKKFLADEFGVTPNKIIKLGESYF
                     THVGMTPHRIHPFVVAAPAEAMKGPNTKFLPFYQLMLLQRLLSKEPHFMLAVARAYRY
                     FHEEMRLDAKRQVKAILQQRFAGQEPDWSIPLNYNSLEVLKAQTQEKLKYQAEVQLKE
                     RLEEKKEDVKKAQHKAFHQQNTPMPIEEKPQDPKPQADEPNYAAEEISPAQEKEFEAE
                     LEAFMDVVENEPDLTPRPEKW"
     gene            38673..39389
                     /locus_tag="PK513_00585"
     CDS             38673..39389
                     /locus_tag="PK513_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOO80984.1"
                     /translation="MQAVDTVLPPRCVVTGETVDQQGMLSPQAWNTLEFITDPVCAQC
                     GFPFDFEVEEGSLCASCLDRPPPFDLARSALKYNDTSRDLVLGFKHGDQTHIARAFVL
                     WLEKAGARILEEADILIPVPLHRWRLLSRRYNQSAIMACALSKAVNIPVEYHALRRTR
                     ATPVQGHLKAAERHKNVKRAFALCPDREKIIHGKTVVLIDDVFTTGATVKECTKTLLK
                     SGAAKVHVLTLTRVVREGFG"
     gene            complement(39434..39604)
                     /locus_tag="PK513_00590"
     CDS             complement(39434..39604)
                     /locus_tag="PK513_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80985.1"
                     /translation="MGSITELCEKSIEPRIPPKKNHKIKSAYDEALYKQRRKVENMLY
                     EIPRRKLLGMTN"
BASE COUNT        10000 a        10652 c         9655 g         9658 t
ORIGIN      
        1 agggcaagtc cggcgttcgt ctcgaaacaa ccgacaatct tcatatgcct ccaccaggcg
       61 atgtgcgcat tgatgacccg caaaagcaga aaagaaccta tgaagtcgct ccggctggta
      121 acgccgtcat catggaagaa cagctcataa attccggtca gacggtgatg gattacaacc
      181 tcatgaccaa tctataccaa aagaacaccg gcatgattta caccgcgctc ggccgccagc
      241 gctaaggcat aaggagggat agataatggg aaccgaactt ttaaacgcta tgcaaatctc
      301 cgccagcggc atgcgcgccc aaggcacgcg catccgtgtc atctcggaaa acgtcgccaa
      361 cgccaataca acgggcgaaa cacccggcgc cgatccttac cgccgccaga ccatttcatt
      421 caaaaatgta ctggatcgtg aggcaggtgt taagaaagtc gaggttaaca agataggcga
      481 ggattacgag gataaattca aactcaaata cgaacccggc cacccggcgg ctaatgaaga
      541 aggctatgtg aaatatccaa atgttgagcc gttgatcgaa accatggaca ttcgcgaggc
      601 tcagcgttca tacgaagcca acctcggaat gattgaacaa tcgcgcggca tgatcatgcg
      661 cacaatcgat ttgctgcgtt aatgattggc gcgtataata aagagcaagg agtaacccca
      721 tggtggacaa gatcatagac ccaaacatag cgatgaacgc ctattcgcgt tcgatgaata
      781 tcgcccaagg ggccaaaggc gcagatgaaa cggactcttc cggcgaaagc ggaggcgctg
      841 gcggcgtcac gttttcagat tttctgaaac aaaaagccag cgaatctgta gatacaatgc
      901 gcaagagtga ggaaatgtct gccagggctg taaccggcga agccgacctc accgatgttg
      961 tgcaggccgt cactgccgcc gaactcaccc tccaaaccgt cgtttccgtc cgcgatcgca
     1021 tgatttcggc ttatcaagag attatgcgca tgccgattta gcagcttgct tgcgcattct
     1081 gttcgattca ggctaacctt ttttcatgaa cgacgaacaa attctcgaaa cagtccagca
     1141 agcgctcttg atcgctatcc agatcggcgc gccggtgctg ctcgttggcc ttgtggtcgg
     1201 ggtgacgatc gcattggtcc aggcccttac gcaaattcag gaaatcaccc tcgttttcgt
     1261 tcccaaaatt ctcgctatat ttctggccat gttcattttc ctcccctcca tgatgaaaac
     1321 tctaagcgtg ttcatggaaa gcctggccga taaaatgatc gggctgggct agcacgtgcg
     1381 tcattgcgag ggaagcaatc cagatatgaa acaaccggct gtttatatat tggcgagtaa
     1441 aagaaacggt acgctttata cgggcgtgac atctgactta gttacaagga tatatcaaca
     1501 taaacaaaaa gcgattgatg gatttaccaa aaaacacaac tgtacattgc tggtatatta
     1561 cgaaatatac gacgatatgg aagaagctat aaaacgcgaa aagcaaatta gaaaagcaaa
     1621 ttaaggtggc tctagaaaga aaaagctggc gcttgttgag ggccagaatg ctcaatggga
     1681 tgatctctat gaaaaaatca cggcatagaa attctggatt gcttcgctcc gctcgcaatg
     1741 acgcagagag gctgtagtgg agtcagccat cgaaacattc ctgaccacct ccgttctggc
     1801 cttcatgttg accttcgtcc gtctgggcag cgcaatcatg atcatgcccg gcgttggcga
     1861 ttccttcgtc tcgacccgca tccgcctgca tataggcgtg gcgctatctt ttgcgctttt
     1921 tccgctggct atgccctata tgcccgatcc gctgccttcg acctttggat tactcgtgct
     1981 aatcgcgatg gaatttgtca ttggcctgtt tatcggcacg attgcgcggg tgctcatgtc
     2041 ggcgatggat accgccggaa tggtgatctc gatctcctcc ggccttggca acgcgcaagt
     2101 ctttaacccc tctatggcca cgcaaggctc gctggttggc gcgctgctct cggtcacagg
     2161 cgtagtcatg ttgtttcagt ttaacctgca tcacctgctg ttcatcggca tcatggaaag
     2221 ctatgagatg tttcctctgg gtgctatccc tgataccggc tcaatggcgg aattaatgtc
     2281 ccgcacagtt tcggccagct tttccatcgg cgtcaaaatt gctgcgccct tcatcgtcat
     2341 gacactgctg atctatgtcg gtatgggcgt tctctctcgg ctgatgccgc aaattcaggt
     2401 cttcattctc gccctgccga tgcagatatt gctctccatt gccttgcttg caatggtact
     2461 ctcagcagcc gtaatgtact ggattgccca gtttgaacaa ggcatggtct ttttcctgcg
     2521 cgcttcgggc ggttaacgct gtcgttatta aacgccccct ctcccttgag ggagagggat
     2581 gctatcgctt atcgaggcaa tgccaagatt agcgtatagc tgggtgaggg ggataaaaca
     2641 aaattcccct tcgaatctcc gcgtaaaaac ttcgcgccct ctgcgtcttt gcggttaata
     2701 taaatttgtg tccatttgcg ttcattcgcg gttaatataa aaacatgagc gacaatcagg
     2761 aacaagacga cgcccagaaa accgaagacc ccaccccaaa aaaactggag gaggctcgca
     2821 agaaaggcca ggtcgcggta tccaaagaaa tcaataactg gcttatgctc tttgctggaa
     2881 caatccttgt cgccctgatg gccccgttcg cgttcacgaa tctgcttgaa atcctcaaag
     2941 cgtttataga gcgttcctat gccttcccct ccgcccccgg cggtatgggt gtaatactgg
     3001 gcggcgggct aaaacaggtt ctaatggcgc tggctctgcc attggctttt ttgtttatcg
     3061 tcgcttttct cgcgccattt gtgcaggtcg gcccgctctt tgcccccgaa gctataaaac
     3121 ccgaaattag caagatttct ccgatcaaag gctttaaacg cctcttttcc atgcgctcgc
     3181 tgatggagtt tgccaaggga ctgcttaaac tcgccatcat tggcgtggtc ggcgtgatta
     3241 tcatttatcc ctactttgac aagttcgaac attttatcgg cctgcccatc ccgctgctga
     3301 tgggagaact taaaagcctc gtcgtgagcc tgatgatcgg cgttttgatc gtgctggtcg
     3361 tcgtagccgt gatcgatctg gtctatcaac gccacgaaca cgccaaaaaa atgcgcatga
     3421 ccaaacagga ggtcaaagac gaatataagc aaaccgaggg cgacccgcat gtcaaagccc
     3481 gactgcgcca gcttcgctct gaaaaagccc gccagcgtat gatgcaggcc gtgccgtctg
     3541 cggatgtcgt catcaccaac ccgacgcact attccatcgc cctcaaatac gaccccgaaa
     3601 ccatggaagc gccgctctgc gtcgccaagg gaattgacga gattgccttg cgcattcgtg
     3661 aagtcgccaa acagcatgat attatcttgt atgaaaaccg gcctctggcc cgcacgctct
     3721 atgacacggt tgaaatcgac gaaactattc ccgttgagca ataccaggcc gtcgccgaag
     3781 tcatctcctt cgtctttaaa caaaaaggaa aattgcacta gacaaattaa gcaaggcgga
     3841 gtgtcacagt gtcgttctgt acagcagggg cttcttgatc cggcttaagt tcaggtgatg
     3901 attgcggagg ctctacaacc gcaggatttg caaccgcaaa attctggaaa ttctcgctgg
     3961 caggattccc cccaataagc atattctgac cttcatctgt aaaacacatt tgcggcgttc
     4021 cccgccccaa ctccctgatc tcaccgaccg gactttgctt tagaatttga tcaagagcat
     4081 acggtgaacg gcccccttca acggggcagg ccaaaggcgc ctcagcaccg gcaacctgtg
     4141 ttccttcaga ctttgaatct ggtacaacaa caccgtgttt ctgttccatc ttcagcttgt
     4201 agtcctcaag cttttgcgtg aattcctcaa tgggcatggt gcgcagctgc tcttcggtca
     4261 agttcatggc ttctgccgcc ttcaacgccg ccccgcgggt tttgtcgcct tcttgcccgt
     4321 cggtcaatgc ctgaccggct tttggatgcc cgtcaggata ggtgccgaca ctaaatccgg
     4381 ccgcgaccag agcgctttgc atcttcctga cttcttcgct gggggcagtg cgcggttcga
     4441 caacctgagt accacgcccg acaatcgcca tcctgccact gagcttcatc tccgtgccgc
     4501 ccgtgatatg gatgacgagt ggttcagatg caggctttgc aggctcgggc tctgttggcg
     4561 cggcaggcgc tgctggggca gcgctaactt caacagaatc cgatttgagc gtatcaaacg
     4621 ctttgcggat tgcgggttca tcgtacttcc caataagctc gcgcacgtta cgcacggcgt
     4681 tcatagaagc ctcgatcatg tacccagaac ggccataagc actcccggtt atatccggat
     4741 gatcaccaac gtctttaaga taaagcgcga cgtcggcaaa agaagaagca tgagggttat
     4801 ctgcgtgtac ttgttttaac agcgctaatt cttcatcata atattgctgt gcgcggttaa
     4861 ggaaattctg cttggcgtct tgtgaaaaat ctctgatttc acctctaagc gtaccatatc
     4921 ctgttttcaa atcttccaaa ttattatagg gcggtccggc ggaaattgcg ttctcaacac
     4981 cctgttgtag tgtgcgggca cgctgagtaa atgcatcaaa ggcattatga tcgaaaagcg
     5041 gcttgcttgt atcaaagacc tcgggacgtt tatcaagcag cgcagacgca tatccgccgg
     5101 tgggcaacac tatttcgtgg ccttcaacat cgaaaagagt attttgataa ccaaaattct
     5161 gggtgagcgc acgatccgtg tctccaattt tatgagataa aacctgcaat tcatcatagc
     5221 cctgatactt ctgctctatg tttgtccgcg cggtggacat atggtcataa gcttccagaa
     5281 attttgcctg agaactgtta tcggcgggaa gcatattggt gaacaacgtg aaggctttgg
     5341 tgtcattatt caattgcgct ctgtcctgct ccggcatggc atcctcatat cgtcccagac
     5401 tgaggagtat ctctcttgca ttaacgtctg ggtgttggga tttaataaga tcctgcaccc
     5461 ccctttcatg actatgcttg tagctgactg cttcttgagg gcttaattga ggtcccccaa
     5521 cagcagaaaa tctgtcatca acatatgctt gaagatcgtt tataagggat tcagggtctc
     5581 cctgtcccgt atcaatggcg tgaagagccg cattgatttt ctgattcaac gtgtcgttgt
     5641 tttgcgaatc tagaagtctc acggttgcaa tttttacgct tgtttcatac gtattgccaa
     5701 atacacttgg atcctgcgcg taaaacgccg tgaaattatc ataggaactt gccgccatct
     5761 ttaccctctt ctcaaataaa actatgtaaa ttgtagaggg aaggggttaa ttttttctta
     5821 acaattccct aacgcactgt tcctgtggta aaaaagacat atgagcctgg atcattctga
     5881 atttatccgc cgccaccata aaacagcgca aacgcaaagc ccgcccgggc ccgttcgccg
     5941 ccgccatgac gggctgttcg ccgtgattct gttcacgatt ttcgccgtgt ttctcggcta
     6001 tatcctcacc cttgcaccgc tctcgcaaga catgaaaatc attacatcga tggctttggc
     6061 tctgtgctgc cttgtcggct ttatccttta tcgcggctat cacaaccacc gccagcttga
     6121 tgaagagctt gagctggtcc gcgaagtcat ggagggctca cgcggcgcgc gcctgatcat
     6181 cgacagcgcc gacaataccc tctattacaa ccaaaaattc gagaggcttt gccgcggcgt
     6241 cggcccgcca agcttttcca gtctgctgcg cctgttcgac tacagcgacg aagtcgccgc
     6301 ccattttcgc cttctcaccg atcaggccca tcgcggccta accgattcga tcgaactgtt
     6361 ctcccgctac gaagatcagg aacgctggct gctcatcacc gcccagccca tcgccgggcg
     6421 tgccggctat gtccattggc gcatcgacga tgtcaccgaa aaacacactg ccgatacggc
     6481 gatccgcgcc gagcgcgaaa aactcatcga cttcaccgac aatgccccgg tcggcttttt
     6541 ctcggtcgat gaaaagggcc gcttcgtctt tgccaatgcc accttcgccc gctggctcgg
     6601 cgaggatttg caaatattgc tcgaatccgg tcggctccac acctacatgc tcaatcctcc
     6661 ggaagacgca aacccctacg acctgatcaa agaaggcacc gcccgccaga tcaccgaagt
     6721 gcaaatgaag ggcacggccg gtaaaacatt cctcgcctcc atcaatcagg tgatcgtgcg
     6781 cgaaggcgct ggccccgatg gaaagcaaag agtgcgtacc cgcgccgttg tccacgacct
     6841 gaccacagaa agcgaaatgc gtcaggccct gaaggcttca gaggaccgtt tccagcgttt
     6901 cttcgacgaa gctcccgttg gcatcatcct cattgatgaa gagggaaaag ttgcagattg
     6961 taacgccgcc ttcgccggca tcatcgaaac gccgattgat aaaatcgaag gacagacctt
     7021 taacgacctc atcgatgaag aagaccgcga cgaagtcaca ggcgcgctgg gccgcattga
     7081 aaaaggccag cgtcttgatg ccccgctcga aatttccctg aaaggcgcca ccggccctgt
     7141 cgaaacgcaa atgcacgcca aaaaattcaa acttgatggc cgcaccgttc tgcattttat
     7201 tgacctgaca gaaaaaaagg cgctcgaagt ccagttcgtc cagtcgcaaa aaatgcaggc
     7261 cataggccag ctcgccggcg gcgtcgccca cgattttaac aacctgctca cggccatcat
     7321 cggcttttgc gatttgctgc tgcttcgcca caaaccgggc gacccgtcct ttggcgatat
     7381 tatgcagatc aagcaaaact ccaaccgcgc cgccaatctc gtgcgccagc ttctcgcttt
     7441 ctcgcgtcag caaaccctgc gcccgcgcat ccatgatgtc agcgatattc tttatgagct
     7501 ctcacacctc attcgccgcc tcatcggcac gaatatagaa ctggaactca ttcacggcga
     7561 agcgcttggc cccgtgaaag tcgacgaagg gcagatggaa caagtgctca tcaaccttgc
     7621 cgtcaacgct cgcgatgcta tggatgaaca tgcgcgcgaa ggtgcaaccc tgaccatcga
     7681 aacgcaaaac tttaccaacc gcaaaaccaa gaagctgatt tccgaagagc tcccgccagg
     7741 ccactgggtt acgattaaag tcagtgatac cggttgcggc attgagaaaa agaacctcag
     7801 tcgtattttc gaacccttct tcactaccaa acaagtcggc gagggcaccg gccttggtct
     7861 ggccactgtc tacggtatta tccgccagac cggcggcttc cttgatgtcg aaagtaaagt
     7921 cgctactaag gacaaagaag gtggcaccag ttttatcatc tacctcccgc gtatcagcaa
     7981 ggatgaagct gaggccgagg aagagcatcc tgaaacagag gaggtcgaag ccaaagacct
     8041 caccggcaca gagcgcatct tgctggtcga agacgaagac gccgtgcgca ccttctccga
     8101 gcgcgcattg accaataaag gctatgaagt cctcaccgca gatagcggcg aatcggcgct
     8161 taacctgatg gaaaaacagg aaaatcaaaa gatcgacctg ctggtgaccg acgttgttat
     8221 gcccaatatg gacggcccaa ctctggccca gcgcatgcgc cagacctcac cagggctcaa
     8281 aatcatcttc atgtccggct ataccgaaga caaactcaaa gaccatatgg gcgaaaacat
     8341 cttcttcctg cccaaacctt tcaccctcaa acaactcgcc gccaaggtga aagacgtact
     8401 gggggattag gtgctgttat tgcgaggcat agccgaagca attcataaat aaaagttctt
     8461 tttttgttct tttttgttga cgagagaaca aaatgtgtac attgttgtcc tcaagattac
     8521 ttgacgcctt ggcggccctg aattgcaacc gctaggaatt tatgcaaaaa aggagagaac
     8581 caatgtccgt aacgccctta agaaaagacg atgaaatgac acaaaattct gccgagaaac
     8641 aaaaagcgct cgacgccgcc ttgtcgcaaa ttgaacgcgc cttcggtaaa ggctcaatta
     8701 tgaagctcaa tggcgaacaa aacaccatga acgtggaagc tatatccacc ggttcactcg
     8761 gcctcgatat tggcttaggg attggcggcc ttcctcgagg gcgtattatc gaagtctatg
     8821 gcccggaaag ttccggtaaa accacgctcg ccctgcatgt gatcgccgaa gcgcaaaagg
     8881 ccggtggtac ttgtgctatc gtcgatgcgg aacacgcgct cgaccccggt tatgccaaaa
     8941 aactcggcgt gaaggtggat gaattgctga tctcccaacc cgatgccggg gagcaagcgc
     9001 tcgagatcac cgatacgctg gtccgttccg gggcgctgga tgtactggtt gtggactcgg
     9061 tcgccgcgct cgtgccgcgc gccgaacttg aaggtgaaat gggcgacgcc cacgtcggct
     9121 tgcaggcccg cctgatgagt caggctttac gcaaactgac cgggaccatt gcccgttcgc
     9181 gcaccattgt aattttcatc aaccagattc gcatgaaaat cggcgtgatg tttggctcac
     9241 ctgaaacgac gactggcggg aatgccctga aattctattc ctctgtgcgt cttgacattc
     9301 gccgtatcgg tcagattaag gataaagacg aggtcgtcgg caaccagacc cgcgtcaaag
     9361 ttgtcaaaaa caaaatggct ccgccatttc gtcaggtcga attcgacatc atgtatggcg
     9421 aaggaatatc caaaatggga gagcttctag accttggtgt ggctggtaac gccgtggaaa
     9481 aatccggcgc ttggtattcc tatgatggcc agcgcatcgg gcagggacgg gaaaacgcca
     9541 agaccttcct gcgggaaaat ccaaaaatgg ccgaaaagct agaaaaacaa attcgtgcca
     9601 atgcggggct cattgctgaa gatatgctca ccggcccgga taatgacgaa ggtccggaat
     9661 taacggcaat cgaaccggaa actcatccgg atgataagaa aaaagcataa attagggtct
     9721 ggacatatgc atccccgcgt aagcggggat ctgatccccc cccccctaaa aaaatcaaaa
     9781 acgaactgaa taactcaaaa agcttttata aaacagtggg ataactagcc tttttcgcgc
     9841 ataatgcgcc ctttttgctt atcccaatcg cgctttttct cggtttcccg cttatcgtat
     9901 tgcctcttcc ctttagccag cgcaatttca atcttcgcca tgccgcgtgc attaaaatac
     9961 agccgcagcg gcacaatcgc gtatccttcc cggctcaccg cgcccagtaa tttatcgact
    10021 tcacgtttat gcaacagcaa tttacgtggc cgctttggct catgctgcaa cgcctggctg
    10081 gcttgttgat actcagggat attcgcatta aacagccaga tttctccatt cttcggcccg
    10141 acataagcct ccttgatatt ggcttgccca tggcgcagtg acttcacttc cgttccatgt
    10201 agcatcatcc cggcctcaaa cttgtcctcc agcgcataat caaaccgcgc ccgcttgtta
    10261 tcggccaccg ttgcattggt cgacagtaat ccggatttct tctttttact catcgtcgag
    10321 taccttcggc aatgccatcg ccaacgactt cataatttat aattgtttcc atcgatctcg
    10381 tcccaactaa atctcatatc ttcgggaatt tgagagcgaa tacccaggct gagaacatgg
    10441 tcttcaattt ggtgaaagct catattttga agattgtgag ggcggcggtg accgtaaaaa
    10501 ggctccaccc cgcctccgta aagagaggcg ctgtgttttt taaaagtgtt cccaaacggg
    10561 cttttctttt tgcgtttttt cttttttggg ggaggggaaa attcagcgaa tttttctgca
    10621 atggtttttt ctatccattt ccggtcgtca tcactgagac tcatatgttt ctcctcgcgt
    10681 tattttttca gaattctcaa aaatacattc ttccgttctg ggttacgttc acgccgccga
    10741 agccttcgac gccaccgaaa gtcccgcatg cgccagcgcc gcatcgactt tcgcacgggc
    10801 ggcctcgctc gccggaatca atggcagacg tagttcatca cgacaaagcc ccaaacgctg
    10861 cgccgcatat ttaaccggcg cgggactgct ctcgcaaaac agatcacgtg ccaacggcgc
    10921 aagcttgtcg cgcagcgctg caaaggtttt aagatcacca gcttcccacg cctcgaccat
    10981 atccgcatgc atgcgcggcg caacattgga tacaacggaa atcacgccat gcccgccttg
    11041 cgccaaaaac ccggccacac aatcatcatt cccggaaagc tgacagaaat ccttgccggc
    11101 catcgccacc agttcagcca cacgcgcaac attcccggtg gcgtctttca cgcccacaat
    11161 atgcttatgt ttagccagtg ttgcgatggt ttcattgccc atatccacaa cgctgcgtcc
    11221 gggaatatta tataggatca tcggcaacgc cgcagcatcg gcaatcgcgg taaaatgcgc
    11281 aatcattccg gcctgtgagg gcttgttata ataaggcaca acgcttaaat gcgcatcggc
    11341 accagcatct ttggctgcaa cagacaattc aatcgcttca cttgtggaat tcgagccggt
    11401 tccggcaata acaggaattc tccccttgac gacatccaca cagcgcttaa ctatggccat
    11461 atgttcatca tgcgacaaag ttggcgattc acccgtagtt ccgcaaggca caagcccgtg
    11521 cgatccttgc tcaatctgcc attccaccag attatcaaac gctttccaat caattttacc
    11581 gtctttaaac ggggtgacca gcgcggtaat agagcctcta aacattgttc gtctttcgtg
    11641 tttcatcagt caaaatcggt ccataatcta tctgctctga cacgcagatg caagaaaatc
    11701 agcgctctag ccgtcatttt cagcatattt attaccgcca gtgcatttcc caaggatagc
    11761 cccatcaacc ggctgcaatc agcgcgcgca caaaaacaga ccattcgcgc actccagctt
    11821 gtccatcagg ggcaatggaa cctggcgcat gatgccttcg ctgaaaccca tgatccgcta
    11881 gcttcaaaac tatattattg gctgattttt tcacaaaaag gtgatttcga aaattatgtc
    11941 cgcctgacac agtttattcg aaacaatccg gaatggcccg gcatagacga actgcgccgc
    12001 agggcggaag aaaaaatgcc ggaaaactta cgtccagagg aaattttagc atggtttaca
    12061 gattacaaac caagtactgc gcgtggcgtt gatcgctata tgtttgcaat gatcaccaca
    12121 gggaaaacaa tcgacgccaa gagcttcctt gccgattggt gggccagcac gacattgtca
    12181 cgcgatgatc agcggatgat tttcaggaaa tacaacacct atctcgatcg cgctgcccat
    12241 ctgcgccgtt ttgataccat gctttatcgt ggacaaaatg tgaacgcgcg cgctttggcc
    12301 ggcgttttag ggcagggcta tccgcaactc gccgaagctc gaatcgccct gcgcgccgaa
    12361 aaaaaatccg taagccatct gatcaacgcc gtaccgcgca gcttgcaagg cgatgccgga
    12421 ttgctttatg agcgcctgcg ctggcggcgc aaaaacaatc tcgatatgcg cgccgctgaa
    12481 atcctccatc gcccgccacc aattgaaaaa attcaaaatc caaaggattg gtggacagag
    12541 cggcatattc tcatccgccg catgatagaa aaacgccaat acagaagcgc ggccctgctg
    12601 gcttccaaac atattcaaaa acccggcagt tttgaatatg cgcaaggaga gtggatggct
    12661 ggctggctga ccttgcgttt tcaagataac cccacacagg cctatcagca cttcgaagcg
    12721 ctctaccaaa atgttcaaac cccggtcagt aagtctcgcg gggcctattg ggccggacaa
    12781 gcggctgcaa aattcacaga cccgtcaatt tctaaagact ggtacgaaaa agccgccaga
    12841 caccaaacaa ccttttatgg ccagcttgca ggcgcaaagc tggggcagga tggcgctttt
    12901 ccgggtgctg cgccgcccaa acttgatgac gccgagcttg ccgattttca gcgcacggag
    12961 cttattcagg ccgcgcagct ttttgcccgt gccggcatgc aagagcaaag cagccgtttc
    13021 ttatgggcat ttgtaaagca tgaagggagt gccaaggcct ataaatatgc tgccgagctt
    13081 gctattaaaa acggtcagac gcacgaagct gtacgtattt cgaaagaggc gactaaaaag
    13141 ggtttattcc tgacggcgca gtcatatcct gttattacac accaattgcg tgatattgat
    13201 ttggagtggg cgcttatcca cgcgcttatc cgtcaggaaa gcatgtttga tgtgaaggcg
    13261 caaagtccgg caggcgctcg cggtttgatg caactcatgc ctgcgacagc cgaggaaaca
    13321 gcacgtaaaa tgggcattgc gcataacacc gtatggctca ccagtaatcc gcagcataat
    13381 attcgtctcg gcgcacagta tttattcgat atgattcggc gttatgacgg tgcctatcct
    13441 cttgcaattg cggcctataa tgccggccca gggcgcgttg accaatggct caaaacattt
    13501 ggcgatccac gtacccgaga ggttgattgg atcgactgga tagaaatgat accgatttat
    13561 gaaacccgta actatgtgca gcgcgttatg gaatcagttt acgtctacag gttgcgattg
    13621 cagaaggttc aaagaccgtc cgaagcgcct cttcatatcg ccctgacgca gcaataaaag
    13681 acttgataaa gcaagggctt aaagaattac ctctgcattt ttatatgtat tttaaataaa
    13741 gcgtatattt tttttatgat tttttcacca aaataagcta aagtataggt tgtggggttc
    13801 gggctttatt tattaagccg cattatattg ggggaaggac tatcatgaag ctgctgctcg
    13861 cagatgatca tacgcttttt cgtgacgcgc ttgttcaata tattgaacgc tctgatcccg
    13921 atgcggaagt atccgtagca aaagatttcc atggggccta tgagcatatg caaaaggacg
    13981 gaaagcagga tcttattttg ctggatcttc gtatgccggg tatggacggg ctgcaaggct
    14041 ttgaaaaaat gcgcaaggcc tttcctgaaa ctccagtagc tttgatgtcc ggcattgcgg
    14101 agcctgaaga tgtccgcgcc gcaatggatt taggcgcggt tggttatttt cccaagacgc
    14161 tttctggaaa agccttgctg caagcaattg gccgcgtttt agagggggaa atttttattc
    14221 ccacagatca taagacgcaa cagttaatgc ccgcatatta caatgatatc ccgctgtcag
    14281 cacctgcctg tgagaataat gtaccgccac aagcaaaaca gccggaaaag aattttaaac
    14341 taacacccag agagcaagaa gttcttaaac acctcacgac tggagcatct aacaaggaaa
    14401 tagccaatca tcttggcctg aaagtggtaa cggtaaagct ccatgtgcgc ggtatttgcc
    14461 gtaaacttga ggcaaaaaat cggacgcagg cagcactcat cgcccgagaa aatggtcttg
    14521 cgctgggcgc agggtaggag cttaagaaag cgcgatgtta aatttttttc gccgctatta
    14581 tcacctcttt ttatttattc ccggtcttat cattctaggt gtagggatgc tcaccttgca
    14641 tacatccata aagctttcat ctgagcaaaa tctgcttcgt cagcactacc agatgatttt
    14701 aacccaacat cctggatttt cagattcggc tgccggacgt aaaataaaaa ttctgactaa
    14761 aggcgtccag actcaagaaa aaaatcttta ttggtctgtt atagcgattg cgctcacagg
    14821 gtttttatta ttgatcataa atgccgatca tctgaggaaa ctggtcgaag ccaataaata
    14881 acggcaaaaa tcgctcaaac ttttagaaca caggttggcg gcgattgaag catctttcga
    14941 aggaattgct atcgtcgacc cgcaagggct cctcacctat atgaacaagg cgttagcgga
    15001 tatttatggc gtagctgagg agcaaaagga agactatatc ggccatttct ggctcaaatt
    15061 atatagcgat gaaaatcagg cttacataaa aaatcacgta ctcccacagt ttgaacgcga
    15121 agggttctgg catgatacat caaaactttt gcgtcgggat ggcagggcaa tcacagcaga
    15181 gttgtctatg acccgtctgt cagatggtgg ctttattggt acggctcgcg atgtaactga
    15241 aattgaaaaa gccagcgccg aaaagaaaga gatacaaaat cagctcgcac aggcccaaaa
    15301 aatggaagcc atagggcgtc ttgccggggg gatcgcacac gactttaata atattttagc
    15361 cgcgataaat ggctatgccg aatttttgac agaagatttg gacgaagata gtccgcagaa
    15421 aaaatatgca gacaatatct taaaagcagg ctatcaggcc cgcgctctca tcgaccagat
    15481 acttgcgttt tcccgccgtc aggatggcga tgttgaaaat attgatttgt gtggaccgtt
    15541 gaatgagagc atctccatgc tgaaagcaac gctgcccaaa aaaatcgcag tacatacgga
    15601 cattcaggtt atgcccgcgc caatagaagg caacgcaaca cagattgcac aagcaattat
    15661 gaatctgtgt gtaaatgcgc gtgacgcaat gcgcgatgat gaaggggttt taagcatatc
    15721 tctgaaaacc gtcgatgtga tgaatgatat tcctatgacg tccgtgagtg atgatctgcc
    15781 tgccatggaa gacacgccgc ttatcagcat tgaagaagga gagcagggcc agacttatct
    15841 gcattttgga acggtaagcc gcaaccatca atatatcaag ctctcagttt ccgataccgg
    15901 cacaggcatg agccgcatga taatggaaca tattttcgag ccgtttttca ccaccaaaga
    15961 cgtgcacaaa ggcactggtc tggggttggc cagtgtacac gggacaatga catcccatcg
    16021 cggcgcattg tttattgaaa gcgtattggg caaaggcacc aaatttgatc ttctgtttcc
    16081 cttaagcaaa gtggatgaac aaagcgaaac agatatagac atgcatgagc tggaaacgca
    16141 tggaggtctt gttttattag tagaggatca agacgaagta cgcgaaatga caagcaccat
    16201 gctcaagcgt ttgggctacg acgttgaaac ggccagaaat gggctggaag cttcagatat
    16261 gctgtgtgaa aagcccggag aatatgatct cgtgctaacc gaccaaaaca tgccaaaaat
    16321 gaccggaact gaactcattc gtcaatccag ttttgatttt cccgaattgc cgtttgttat
    16381 tttgacaggc tatagcgagg aaaagcttct tgaatttatg caagaacaac cggctctaaa
    16441 agacgttttg cgcaaacctg taagccagaa gattttggcc aaaacgctgt ctgaggtttt
    16501 aaaaggaact gcgaacagcc caacgaaaaa agccgccgct atttagcgtc tgcgcccttc
    16561 ataaaaccat gtcccgagca tcagcgcgag tataagcgtc aaggaaagcc aaagcgtgat
    16621 cgcaggcttc tcctcggcgc caatcacatt gaaagcctgg gtatcttgca atccaatcca
    16681 gtcttgcccg ccgtaagggc cgtggcgcat catccgcaga cgtgggcgcg gcgtatcttt
    16741 aagccacaag gcttttcccc ggctatgctc caaaagcggt tgaagcaatt cagcgctact
    16801 caaaacattg cgaaattccg gcggatcaac ttcgccaacc accgcaaaac gtaaaccgcc
    16861 atctgcggtc tcaaaacgat aaatgccgtt ttgtttggcc cggtggaaat gctccagcaa
    16921 accattttgc gtaggggtta aagcaagttc ttcttcacgg ccatcgggcg cgcgcatttt
    16981 gacaacatct ttgctgcgtt caaacgccgc ccggcgtaaa atcaatgtat gcccgtctac
    17041 gcgtacatcc aaaatctgct catcaagctc cggctctttc atcagccagt gcacgacgcg
    17101 ccgtaacagt tcgccgtgtg gcccgccgcc ttcatatccg cgtgaccaca gccagatatg
    17161 atcactggca atctgcgctg cgcgcccctt cccaacatga tttaagatca acaaagggcg
    17221 ctcatcaatg ccgtgcatca acacatgccc tttaacattc tgcaagccaa tctgacgtag
    17281 ccatggcccc cagggcgttt ttttgtcttt gcgcatgctg ccgccccccg accaaagtaa
    17341 actgcgagtt accgggtgac cctggccgag agttgtgatt ttgggagtat aggcctgctc
    17401 ataaacctgg ccattgggaa cgcccggaag aatgtcgcgc agcgccgtat aataaatcga
    17461 atcttcgctg gcataggccg gaccgctggc ctccaaaaag gctccgcctt tctcaacata
    17521 gcgcgcgata ttacggaaat aatgatccgg caaaatacgg ttcagtcggt atcggtcaaa
    17581 aatgatcaga tcaaagtcat agagtttaag ttcgaacaat tcacgaaatg gaaacgcgat
    17641 cagcgacatt tcattttgcg gcgtggcatc gactttatcg ggctcacgca aaatcgtgaa
    17701 atgcaccaga tcaacactgg gatctccggt cagaagatcg cgccatgtac gcccgcccgc
    17761 atgcggcttg cccgaaacca gcaaaacgcg cagccggtca cgcacacctt gaaaatcaat
    17821 tgccgcccgg ttgttgcgtt ctgtcagctc gccaggcaaa ggctcaacaa aaagttcaaa
    17881 cacattttgc cctgcatgat gcaccggcag gctcatcgtg tgctcttcac cgacgcgggc
    17941 tacatcgcgg cgtaccgtac cgtcgctcat ttgcaagctc acctgcacat gcgtttcgtg
    18001 caaagcgccg ctatcctcta tacggaaccg tacatcgatg tcctgcccga caatgccaaa
    18061 ggccggggca ttcgtgacaa caatccgcct gtccgtttcg ttcttttcac cggtgagaag
    18121 cagatgaacc gggccgtagg attcttgaag cttttcaaca tctggaatgt cgtgaacttg
    18181 cccgtcgctg atgataataa cgcctgcacg ccgcccttcc ggcacatcgg ccagagcggt
    18241 ttctaaggac tcaaacaagt tcgtgctgct ggctagcgcg ccatcatgtg gtcccttcac
    18301 caaacgcatc tctaaggatt cattacgctc aattgtattt tgcaaatact ccagagcatc
    18361 aagactgcgc actttgcgtt ggccgaaatc ctgactggcg ctttcatcaa ggacaacggc
    18421 aacgacgctg ggaacagcct ctcgtttctc ggtatgcaca gacggcccgg ccaaaataaa
    18481 gagaaagaat gcaaaacaca gcgcacgaaa aatcggaaag cgcccggcgg taaaaatttt
    18541 ggccaatacc agcaccagcg ccaacaatcc aaacgtacaa agcatcgcct cagggataag
    18601 aggagtgaaa tgcaggctaa gcgattgagc acttaaatta ttcacgggtg gctccttccg
    18661 tttctggcag ggctgccgcg tcccaaacgc tccaggatat gtggaacatg cacttggtca
    18721 gctttataat tacccgtaag cgcatacatc acaaggttaa ccccaaagcg cagcgattgc
    18781 tcatggcgcg gagtcatgcc gctcaaatat tgctgccccc cacgcgtaac gatattaagc
    18841 tctgcccagg cgccaaccca gtcatgagag ccgagaataa cggaagacac gccatcccgt
    18901 ccgcctgcac tttgtccctc aacccaaagc ggcgcgcctt catagcgccc gggataactt
    18961 ttcagcaggt aaaaagtttt attcaaaaca tggtcttccg gtaagggaga aagcggcgga
    19021 atattcaaac cgcttaaaac cttgcgcagg gcaggcgatg atttctgccc gtcgcgcgta
    19081 tcgactaaaa tactgccgcc atgatcaaga taagcctgca ggttttgcag tgcttcggcg
    19141 ccgggcggtt cttgcgcggc gctgatcggc caataaagaa gaggatagaa tacgagcgta
    19201 tctgtttcca gtgaaacccc gcgcacatgt ttgggttcag ccgaagtgcg catattcaaa
    19261 gccaccgcca gaaccttcag ccccttttcg ctgagcgcgt caatactgac attgcctgtg
    19321 cggacataag ccagtgttaa gtcttgcgat tcttgcggaa tttgcgcacg ggccggagcg
    19381 gaaaaagcca gcagtatcaa taccgcaacc gtttttccct taaaagataa gcgcggccat
    19441 tttgcgctcg aaagaaacag cataatcagc gcatcgagca ggaataaaac aaaggctgcg
    19501 ctcagcaaaa gcggcgcaag atccttttca aatgcccggt cataggtttt aagcaccatt
    19561 ccatcaccgc caagacttgc acgcagcggt ggcaaatgat caccgagatt aagcgctttt
    19621 tgcaaaccgc cgcgcccata aaaaccgggc ggatgttgcg gcattggcgt ttgtttctca
    19681 aagtcagccg catcaatcgg caaaacatgc gccgccgggc tttgcaaact gccaaatccg
    19741 tcaagcattt gaaccggctg gagcatgccg ctttgatcaa gactcaaagc cccgctatga
    19801 cctgaaaatt ttaacaaatg tttgagaagc tggacataaa atcctgaaag cggcaggttc
    19861 gaccattccg gcgaggccgt cgtatgaacc ataacgatca gcccgccgcc acgctgcgcc
    19921 gccgtgatca gcggcgttcc gtcttcaagc tgcatccatg tcttgtcctc cagatcggaa
    19981 gaaaattccg gcaggatttg ccccgatacc cgcaaatctt catccgcatc caaaccataa
    20041 agcgggctgt ccggagcaat cggcgtggcg gcaagaggct tatcccaact cagagagctt
    20101 ttaacgttcc gcgcttcgct gcgcaaaggc gtcggcacaa gtgtgtcttg cccgcgcgcc
    20161 tgtgttgtca tggttggtcc ggcaaagcgc agcagcaagc cgccagcctc gacccacgca
    20221 cttaacccgt tgagagtttc cggcggaaga acgccaatat caggcaacac aatcattgac
    20281 ggatcttgat ctataatttc accaagcggc ccttgtaaaa gcgtggtgta aggctccaaa
    20341 gctttaatca ggtaaaaact gtcttcggtc agttgcgacg cctgcgccgc ttcttccggt
    20401 gcgatgatag ccactgtttt cggtccgccg cttgcgtcca tgaaataatg cgccgccgct
    20461 gtattttgtc cggtaatacg aaatgcgctg gcctgatttc tttgggcctg aggcaaatca
    20521 aatgaaacgc taacgggaaa agattgcccg tctgtctcca cgagcttgtc atcaaggatt
    20581 ttcccttgcg cgcttaaaag ctgcacccgc accggtgcgt tcggctccag attatgcggc
    20641 ccatcaatca taattgacgg ctggctatgg aacttctccg ggctttttag actcaagggc
    20701 aaagagccct gcgccggaac gtgaaatatg acttttccat tcttgctctg cgccgccaag
    20761 gccctgatca aggcaggttg tccgggtttt tcaagcccgt cactgagcca taatacgtgc
    20821 gcatcggaaa gatcaggcaa aatgccgcgc agcgctccaa gtgctgcagg ccaggggtat
    20881 ggcttaagag cgcgcacgaa tgacagcgca tcgctcaccg tcaccggccc atagacctgc
    20941 ggggcttttg tatcggcttc aggagcgctg gtaagaatag tgatattttg tttttgtcgt
    21001 cccgcctgtt caatttcgct gatggccgcg ctttgaatgt caccccagtt ctgcgcagcg
    21061 ccccagccgt tatcaatcat tagcaaaagc ggcccattcg tgcctgcgct ttgcgaaggg
    21121 ttcaacaccg gccgcgctaa cgccagcagc aataaagccg ccatcaacag gcgtagcagc
    21181 aaaatccacc acggcgtatg gctcggcgta tgttctttcg gctctaaccc gtccagtaaa
    21241 cgtacagctg gaaaggcgac cgttttgggt actggcggtg taatgcgcag caggatgtaa
    21301 acaacaggca gggccagcag ggccgttaaa gcccaagggt tgagaaatgc cagcgatccg
    21361 aacagcccgc cgatcattgc gcattctcca gccagatctg cagcaccgtt tcctccagaa
    21421 gcctatcgct ggaatgcaca ataaacttcc agccccagcc ggcggccaat gcttgtaaat
    21481 cctgaagatg cgcttccatc cgcgtctgat aagcctcgcg aatatcgcgc gcattatcaa
    21541 tcatgacgcg ccgttcgcct gccatgtctt caaaatgaat gcgcccctca taaggcaggg
    21601 ccagttctgc tggatctaaa atcacaacca tccagccatt gcgtgtgcat tcggccagcg
    21661 gtttaagcgc cgcccttata tttgcaatag gttcgagaaa gtcgcttaag gcataaaaat
    21721 gcgcatggcg tggaatagaa atcgaccggg gcagggcgca tggatctgtg cgcatcaaga
    21781 tatgttcaaa gctgctcagc gttttttcag aatggcctgg tcgctgcgtt ccggcaaaac
    21841 cgatcatctc tccgccgcgg ctatggagta gcgctagcgt cagagctagc acaccggcaa
    21901 catgtttttt atcaaaacga cacgtatcgg atttaaaatt catatctgcg ttgcgcttgg
    21961 tccagaaaag acagctttgt gcgttatgct gctctttctg gcgaataaag attcgatccc
    22021 ccttcgcgct ttgccgccag tcaattgcgc acggcatatc gcccggctga tattcacgga
    22081 attgccagaa cgcttcaccc gcaccggggc gtttgcgcgg attggccccg tgcaaaacat
    22141 gaaacgcagt tttaacggcc agcgcccgca aatcgggaat ctgcgcagca gcgcgttcag
    22201 cttcagatct taaggggagc aagcgcatga gcttataaat tttcgcttaa gttcctgata
    22261 atatacgctt cgtccaaagc ttcgctatga gctttataat ttagcgccat gcggtgttgc
    22321 aaaaccggca cggcaagcgc aatcacgtca tcaatcgaag gcgcgctgcg cccgtcgagc
    22381 aaagcccgcg cccgtgtcgc cagcattaaa gcctgcgaag cgcgcggccc cggcgcccag
    22441 ctcacatact cattaaccaa ttcaggagag ctctcaccgg gccgggcaga gcgcaccagc
    22501 ttcaaaatcg cttcaaccac ttgttcgcca atcggtatcg cccgtacgag agtctgtata
    22561 ttcagcaaat cctgagcgct catcgcttgc gcggcgctgg cttgatctgt gcctgttgtg
    22621 gccaaaataa tatcgcgctc tgcttgttcg tcgggatatt caacatcaac ttgcatcagg
    22681 aatcggtcaa gctgcgcctc gggcaagggg tatgtccctt cctgctcaat cggattttgc
    22741 gtcgccaaaa catggaatgg ttcgggcagc atgtgagttt ctccccccgc actgacgctg
    22801 cgctcttgca tggcttgcag cagagcgctt tgtgtacggg gtgaggcgcg gttgatctca
    22861 tcggccatca gtaattgtgt aaaaaccggc cccttgataa agcgaaaggc gcggctgccc
    22921 ccaccttctt ttgagttgtc atcttccaaa acttccgagc ctacaatatc ggcgggcatc
    22981 aaatccggcg tacattgtac ccggttgctg tccagcccca gcgctgtcgc tgccgtatca
    23041 accagcaggg tttttgccag tcccggcaca ccgatgagca gcacatgccc gccagaagcc
    23101 agcgtaatga gtgtgttggc cacgacatcg ctctgtccga ttacaatctt ctcgatctcg
    23161 gttttggcag cctgcagttt ggcagcggct tcgtcgaaat actcttcagg tttaggggcg
    23221 gctttggtca cggaaaaact cctgctttga atgcgaacaa aaagtatatt aagggtatta
    23281 agagatttga aaagggtgaa cgcacattta tggaccaaaa acctgtatcg atgttagagc
    23341 cgcaagactg gatgaaggca gacgaaacgc agagcgtcat gaaggcgctg gggcaggggt
    23401 atgccttgtt cgttggcgga tgcgtgagga atgcgctgct cggacagtct gtcgatgata
    23461 tcgatatcgc cagcgcctta acgccagacg aagttacaaa aaaactggaa gccgctggca
    23521 tcaaagtgat tcccacagga atcgatcacg gcaccgtaac cgctgtcacg ggcggcaaac
    23581 cgtttgaaat taccaccttg cgcaaagatg ttgaaacaga tggtcgccgt gcggtggtgg
    23641 agttttcaac cgactgggca gaagacgccc gccgccgtga tttcaccatg aacacgctgc
    23701 tgatggatct ggaaggaaat atttacgatc ctttgggctg cgggctcaaa gatctgcacg
    23761 cccggcgcgt cgtgtttgtc ggcgacccgc gcaagcgcat tgccgaggat tatttgcgca
    23821 tcctgcgcta tttccgcttt cacgcgcttt atgctgatgg cgcgcctgac gcgcaagctc
    23881 ttgccgcttg ccgcaccgcc gccgataaaa tcgccgccct gtccaaagag cgtattacgc
    23941 aggaattttt caaaatcctt tctgttgatg atccgctcac ggttttaata ttgatgtttg
    24001 aaaatgatat tttgaaagaa tttaaaacgc ctgaatatga tgagaggctg ctcaaatccc
    24061 tttgttattt ccaaaagaat tacgggcttg tctccatcgc cgcgcgcctg ttgattctcg
    24121 ccggattcgc agaaaaaaac ctcaaagcct tcgatgattt tcttttgatt cccaaagtct
    24181 tcaaaaaaga tattgagaat ttgaatcaag ttttggccct gccggatctt aaagacgatc
    24241 acgccgttaa agtcgccata tataaacatg gccgcaccgc caccgcgcaa ggattgatga
    24301 tcgaactggc tctcgaccgc gtcatgaacg gctatgcccc tcaggccgtc aaaattattc
    24361 agaattggga tattcccgat ttcctgctca ccggcgaaga cctcatcaaa gaaggtttca
    24421 aacccggccc acagctcggc gaagaactcc agcgccgcga agacgcgtgg attaaaggtg
    24481 ggtttaaaaa ttaaagcccc atgaacctta cttgttccaa acccctggcg cctcttcgag
    24541 agcaacgccg ttatggcttg cttcgacaac ccgataatat gtgccatctt gatctgcaag
    24601 gtgtgtgccg tctacgggct ttaaaacaag aggttctgtg gctgtttttc gatcttcatt
    24661 taattttttt gatgctgcct caaattttga actcatttta attaatgcct ccgccgctta
    24721 ttattttcaa taatagtata aaacaggggt gtgaaatagc atgcctcgct caagaaatgc
    24781 aacaaaaaag tataaagtgg tattatttat taactttgat gcctacggcc acttcacttc
    24841 cgggggcatc gacatcagga tcgcctcagt gtttccgccg gttttcaggc cgaattgcgt
    24901 cccgcgatca tacagcaggt tgtattcgac gtagcgtccg cgcttgatca gttgaaattg
    24961 ccgctcttct gcgctccagc cttcattcat gtgcgtacgc acgatttgcg ggtaaatcgc
    25021 cagaaaggtc cgcccaacat cctgtgtata ggcgaaatcc gccgtccaat ccccgccagt
    25081 gtccgaagaa tcgagataat catagaaaat cccgccaacc ccgcgcggct cgccgcgatg
    25141 cggcaggaag aaataccggt cgcaccattc cttatgcgcc ttgtaataat tcggatcatg
    25201 cctatcacag cattcttgca aagcgccgtg aaacgcgcgc gtatcatctt cattcggaaa
    25261 catcggggtt aaatccgccc cgccgccaaa ccatcccttg gaggtcacaa tcatccgcgt
    25321 attcatatgc accgccggca caagagggct ttgcatatgc gccaccagag aaatcccgga
    25381 cgcccagaac tgcccgcctg atttgttggc ccccggcacc cgcgccgcaa actcttcgga
    25441 aaaaacaccg taaacctctg aaatattcac cccgaccttt tcaaacacgc gcccccgcat
    25501 gatagacatt tcaccgccgc ccttcaggat agaacccgat tcattcggct cgtcgttgtt
    25561 ttcccgggcc cacgggctgc gctcaaacgc gccgggcgct agatcggctt tattgctacc
    25621 ggaaagctcg ccttcaatcg tctcaaactc tgcgcaaata ttatcgcgca accgcctgaa
    25681 ccagacgctg gcctgcgcct ttcgttcatc ccaatcgttc atacggcgct ccatcgcgtt
    25741 tgccttaaag attcacccaa aatcatagaa ctcgcattga caatattcaa cgaacgcatc
    25801 accccatgca tcggaatgag cactcgcgca tcgaccgact catgcacctc ttgcggcacg
    25861 cctgcgctct cgctgccggc cagtaaaata tcatcatcac gaaattcaaa atccacatag
    25921 ggcacagccg ctttcgtgct cataagcaca acccgtcgcc cctcatgcgc accggtaaag
    25981 gcatcccatg atgtgtggcg cacaatattg acatggtcaa tataatccat agccgattgg
    26041 cgtattttac gcacgtccca cggaaaacta cacggttcaa taacttccag tcccacattc
    26101 atacaggcgc aaagccggat catggccccg acattttgcg ggatatccgg ttggtaaaga
    26161 gcaattgaga gcataatgac cgcggtcctt catttcattg tgtgtaattt ataaaatata
    26221 tcgatggaat agtgatagag aaggcttgat catttataaa gagagaagta aaaacaatgc
    26281 gttcgaacat tcctaaataa acaaaaaaca gataaaatta tgacagcaga tagcaaagac
    26341 cggcgcgatt tcctgcaact tacaggtgct gcttttggcg ccgtgggaat agcctgtgcc
    26401 gccgcgccga tgatcaattc cctcaaccct gccaaggaca cattggcatt ggcgaccacc
    26461 gaggtggata ttagcggtat tccggttggc ggttccaaaa ccgtgatgtg gcaggggaag
    26521 ccggtcttca tcaaacaccg tacggctgag cagatcgaag aagccggaga ggtcgatatt
    26581 tcgacactaa ttgacaagca aaccgacgaa cagcgtgtaa agaagcctga atggctggtt
    26641 gttgttggtg tatgcaccca ccttggctgt atccccggcg ggcaaaaacc cactgatgcg
    26701 cacggcgaat atcatggctg gttctgccct tgtcacggtt cgcactacga cacatctggt
    26761 cgtattcgca aaggcccggc cccaaagaac ctcatggttc cgccatatga gttcatcagc
    26821 gatactgtga tcaaaatcgg ataaaaataa gagggtataa ggagaaaacc aatgggcagc
    26881 actgaacgcg cagaattcaa aaaccccctt ataggctgga tagacgaacg tctcccggtt
    26941 ttcacgttaa tgcaaaaaga atatggcgta tttccaacgc ccagaaactt taactatttc
    27001 tggaactttg gcgccattgc catgttcatg ctgatcacga tgatcgtcac cggcatcgta
    27061 ctggcgatgc actacacggc caacactggg ctgtcctttg attcggtcga gcgcatcatg
    27121 cgcgatgtga acggcggctg gttgattcgc tatattcaca tgaacggcgc atcgttcttc
    27181 tttgttgcgg tctatatcca tattttccgc ggcatgtatt acggctctta caagcgcccg
    27241 cgggaattgc tatggattct cggtgtggcg atcttcctgc tgatgatggc caccgccttt
    27301 atcggctatg tgctgccatg gggccagatg tccctgtggg gcgccaccgt gatcaccaac
    27361 ctgttctcgg ccattccgct gattggcgac agcatcgtga cactgctctg gggtggtttc
    27421 agcgtcgata acccaactct gacgcgcttc ttcgcgctgc actatctgct gccatttgta
    27481 atttttgcgg tcgtcttctt acatgtctgg gcgctgcatg tcactggctc taacaacccg
    27541 ctgggcctcg aaccgcaatc tgaaaaagac acgctgcctt tccaccctta ttacacaatg
    27601 aaagacagct tcggactggt tgtatttctg gtcatctatt gcgtctttat cttttatatg
    27661 cccaacgcgc tgggccaccc tgataactat acgcccgcca acccgctggt cacccccccg
    27721 catatcgtgc ctgaatggta cttcttgccg ttctatgcga tcctgcgcgc gattaccttt
    27781 gagattggta tcccttttac ggagatcaca atcatttcgg ccaaactcgg cggtgtgatt
    27841 gcgatgtttg gctccatcct gctgtggttt gtgatgccgt ggctcgacag tcacccgatc
    27901 cgcagcgcgc gtttccgccc gctctatcgc ttgttcctgc tgctgttgct ggttgacttt
    27961 gtctttctcg gctacgtggg ttcacaggcc gctgacgcag agatattcca cattccgctc
    28021 agctatttcg gccttggtgc aaccggctat tatttcgcct tcttcatcgt catcctgcca
    28081 tggctcagca agtttgaaaa aggtcgcgaa ttgccgccgt ccatccatga agccgttttg
    28141 gccaaacacg caaaataaaa ggatattaag aacaatgtat aaaaaaagtc tcctcatcct
    28201 ttttgttctg ctggttctac cggtttccgg cgcgtttgcc tctggcggtg aaacggttca
    28261 cccacccagc caaagctgga gctttgacgg tccgttcggc acgtttgacc gggctgcgat
    28321 gcagcgcggc ctcaaagttt accgcgaggt ttgttcggcc tgtcattccc tcaagcgtgt
    28381 tgcttttcgg aatttgaccg atctgggcta tagcgaggat caggttaagg cgattgctgc
    28441 ggaatatagc gtcatggatg tgcctgatga agagggtgag gttaatgagc gtacggcgtt
    28501 gccgtccgat cgctttccct caccataccc gaatgataac gccgccaagg ctgccaacaa
    28561 tgccttgccg ccggacctgt ctttgattac caaggctcgt catgacggac cgaactatgt
    28621 ttacgcaatt ttgaccggct atgaagaagc gccggcagag gtagaacttc tcgacggcca
    28681 gcattataat gaatatatgt ccggccacgt tatcgccatg gccccgcctt tatccgatgg
    28741 cgctgtccag tatgaagacg gaagcttgca aacaactggg caatatgctc acgatgttgt
    28801 gcaattcctt acctgggctg ctgagccgtc tttagaagcg cgtaagcgca caggcgtgaa
    28861 agtcttgctg tttctgctgg tttttgcttc tattttttat ggaattaaaa agaaaatctg
    28921 ggcaagtgta cattaggttg tgccttcact aggctttact aatgaaaagc tggtagtctg
    28981 ataaaaacga atgtttccgg ggctcattcg tgaatatgta cttgcatttg tacggggata
    29041 gttaaaatgt caaccaacgc ccaaaagact gctcaaatcg acaaggaatg tgcagataag
    29101 acatgtgcaa aagaaaagct tattcgcgcc aagttttacc ggtttatggc gataggttgc
    29161 atgttgtctg cattcgtcat ctctggctat atctttgaaa ttttagggca tggaaatccg
    29221 gcctacattg tccacaaccc tcttgttgtt cttttgttgc ttttaccctt tttaccagcg
    29281 gtatttatgg ctatcctgtc gaagagtagg cgagcgcaag ctatcaaaat tttacagcct
    29341 tactatgata aaaagaatgc aagcgatgtg cgctgtctca aggaaagcga ataacacctc
    29401 caacaacaaa gtattttcct atgcgtgata cacagcaaga aattatccac ttctggttcg
    29461 aggagctaga accccaacaa tggttccaat ccagcgcggc tgttgatgcg gaaattttag
    29521 agcgcttcgc ccttacacac gaaatggccg atgatgggct ctgtaatcac tgggcggtgg
    29581 atgcggaagg ctcgttagcg cttgttattg ttctggatca attcccccgc catatgtatc
    29641 gcggccagcc aaaatctttc gccagcgatg agcgcgcgct tctggtcgcc aaacaagccg
    29701 tgcataaagg atttgatcag atattagaac cggtaaaacg cggttttctt tatcttccat
    29761 ttcagcacag cgaatctctt tccgatcaaa tcaaatccgt tgaactgttt ggtgccatgg
    29821 ccgaagacaa ccctgccggg gatatgtatg ccaggcgtca ctacgtaccg atcgaaaaat
    29881 ttggccgttt tccccaccgg aataaaattt taggacggga aagcaccgcc gaagaaatcg
    29941 agtttcttaa aacgcataag gggtttttat gagcaagtat gaaaacacac taaaagcgct
    30001 gggtgtaaga acagacaaag caggatcttt agaagtcttc tcccccattg atgactcgag
    30061 cctcggtaag gtaaaggagc acagctccag cgatattgag gccattgcca ggaatgctca
    30121 aagcgcattt gaagactggc gcaaagttcc ggctcctgtg cgcggcgaat ttgttcgctt
    30181 gctgggagaa gaactgcgca cggccaaaga tgatctcggc aaactcacaa ccattgaatg
    30241 cggcaagatc ttgtccgaag gccttggcga agtccaggaa atgattgata tttgtgactt
    30301 tgccgtgggt ctgtcgcgcc agctctacgg cctgacgatc ggctcggagc gcccagagca
    30361 ccatatgcgt gaagtctggc agcctttagg ccctgtgggc attatttccg cctttaactt
    30421 tcccgtcgcg gtttggtgct ggaatgcggc tatcgcactg gtttgcggca attctatcgt
    30481 ctggaaacca tcggaaaaaa ccccgctcac cgcactggcc tgccaaaagc tgtttgacaa
    30541 agccgccgct aaattcaaag tatccggcca tgacattccc gatcacctct cacaagtttt
    30601 gattggcgga gcgcaggttg gccaagccat ggtcgatcat ccggcatttt cgttgatcag
    30661 cgccacaggc agcgtcccga tgggccgtgc agtggggcag gcggttgcca aaaggctggg
    30721 ccgcagcctt ctggagctgg gcggtaataa cgcgcttgtt gttgcgccaa gtgccgatct
    30781 tgatatggct gtgcatgcca ttctttttgg cgcggttggc acggctggtc agcgctgcac
    30841 taccacccgc cgcctcatcg tacatgagga tatatatggc tcgttaattc cggcgctcca
    30901 aaaagcctat gaacaaatcc gcgtcggcaa cccgctgaaa gacggcacac tggtcggtcc
    30961 gttgatcgac catggcgcgc ttgatcagat gatggaagct cttgaagagg cgatagagca
    31021 gggcggtaag attttggcag gcggtgagcg cgctcacagc gatgagccgg gcacctacgt
    31081 ccatccggca ttggttgaga tgccaaagca gaccaaaact gtttgtgagg aaaccttcgc
    31141 gccgattctc tacgtcatga aatataaaac gctcgacgaa gcgattgctt tgcaaaacgc
    31201 cgtgccgcag gggctatcct cggccatctt taccaatgat gtccgagaag ccgagcgctt
    31261 tctatccgcc acaggcagtg attgtggtat cgccaacgtc aatattggca cctccggcgc
    31321 ggaaatcggc ggcgctttcg gcggtgaaaa ggaaacagga ggagggcgtg aaagcggctc
    31381 tgacagctgg aaaacatata tgcgccgtca gacccaaacc gtcaattatt cagatgaatt
    31441 gccgctggcc cagggcgtgc ggttcgacct ttaatattca tagtgggaga gcaatcatgc
    31501 gtaatatcct gatcattata ctccttgttc tgagcgggcc ttcgactgcc gccgagatat
    31561 catgtcttga aagggtttaa aggctgcatc cataagattt aaaaaaccac agatcaatgc
    31621 agatgaacac ggtttttttg ggggtgtcat tgcgaggaag gccgaaggcc tgacgcggca
    31681 atctatccct gaaatatgta caagagatgg attgcttcgc gtacgctcgc aatgacgaaa
    31741 agaggttaat cttcttcaga cgaagaagaa aacttctgtg catcgccaag aaagtttgaa
    31801 aaatcaaacc cgccggagct ggaagaatca tcgctttcct caggttgaga caatgacgcc
    31861 tgctgctcgg cgaggcgctg ggcttttact tcttcagggt cttttccttg cgccttcagg
    31921 cctttatccg aagccttctc atattcctgc cacgtgcaca gccccagctc tgccgggtgg
    31981 cgtggccgta aattcggcgt atttggatga gtacgctcac gcactgccgt gatggttggc
    32041 ttggttgtgc cgacaagctt gcaaatctgt gcatcggaaa tttcggagtt gtgcttgaga
    32101 atataagcaa tcgcatccgg cttgtcccca cgcttggaaa caggtgtata acgcggtccc
    32161 ttggcacggg tcttcacggg aggcaagctg cttttcgcaa tttttaaatt cgctgatgga
    32221 tctgcctgac agcgctccag ctcttcctgc gtgagttcat ggctttccat cgggttgcgt
    32281 cccacaagcc cgcggccaat ctcgccatca gccagcgcct caatttcaat ttcggtcagc
    32341 gccgtaaatt ccgcaatttg cctaaatgtc aaagccgtat tttcaatcag ccagacagct
    32401 gttgcttttg gcatcaataa ttgtccgctc atattcactg cagttgccat ggtaaaaact
    32461 ccacaaaaat ttgttctcgc cctcgttcca aaaggccagt ttataaacat agtatttaag
    32521 ggaacgcccc atatataagc gcgctttcat aaaattacca gaaaaatttt aaattgattt
    32581 cgcttgctgc catgcgtatc gttttctaag taatgtttga tgatgacctg ccaaagccca
    32641 aagtggctga atttccccgc aatttggaaa acatgtcggt ttccgaacgg gaagaatata
    32701 ttgaggaatt agaggccgaa atcaccaagg tcgaaacaga catttccaaa aagaaagcct
    32761 ctcacgaagc cgccgcttcc atatttaaat cctaatatta aaggtttgcc atgcgcattg
    32821 aagaaagcac gaaactcgat tttaaagatg tcctcatccg gcctaaacgt tcaacgctct
    32881 ccagccgcaa gaatgtcgat attgctcgtg aatttacgtt taaatggagc ggtaagaagt
    32941 tcaatggcgt acccatcatt gccgccaaca tggacggcgt cggcacattt gccatggcca
    33001 aggccttcgc cgaggacgga aatggcttga gcgtagcgct gcacaagcat taccccctca
    33061 aagaactgca aaaattctac ggtgatgccg gtcatggctc ggtctggtat tccatcggca
    33121 tggtcaagga agatgaagaa aaactcgatg agtttatgaa ggctggccac catccggata
    33181 aaatctgcgt tgatgttgcc aatggttatt ccgaacattt tgtcgatttt gtcaaaagca
    33241 tccgcaataa atacccggat ctgaccatta tggctggcaa tgttgtaacc ggtgaaatgg
    33301 ttgaagaact catcctcgca ggcgttgatg ttgtaaaagt cggtatcgga ccgggcagcg
    33361 tctgtacaac acgtaaaatg actggagtag gctatccaca gctctccgcg atcatcgaat
    33421 gcgctgatgc cgcccatggc cttggcggtt tgatctgcgg cgacggtggg tgcaccgtgc
    33481 cgggcgacat cgccaaagcc cttgccgctg gcgcggactt cgttatgctg ggcggcatgc
    33541 tcgccgggca tgatgagagc gaacagaaaa ttgtcaaaga gggcggtgaa aaattcttgc
    33601 aattctatgg aatgagcagt tcgaccgcga tgaaaaaaca taaaggcggc gttgcggaat
    33661 accgtgccag cgaaggcaaa acggttaagg ttccataccg cggtcccgtt gaagccagtc
    33721 tgcaagacat tcttggtggt ctgagatcaa cctgcaccta tgttggtgca cgccagttga
    33781 aagagctttc aaagcgcaca acattcattc gtgtatccca acagctcaac accatttttg
    33841 gaaatgaata aattacctct ttaggaaggt tgcgtatcga cgaagcgctc gttaatctgg
    33901 tatctcattg agtgaaacag ggagacacgc catgtcagat gaagtaaaac agcgcttaca
    33961 agacagcact gccaattgtt tgaaagccta tgatgcctgg gacggagaca agaaaaatgc
    34021 caccgcgcgc gaaactctgc aagacgccgt ccatgagctg cgcaaagtcg cctcgcgtct
    34081 ggaaattgaa attgctgtaa gtgatcgcaa gcagatgaca caaaaaccta tgccgattcc
    34141 cgcccaccgc gatgccagag gcgcgcaaaa gaaagatgat gccgacctga gcaaaggcaa
    34201 cgaaaaaaag tctgataacc ctaagccaaa gctcaaacct aaaaaagatg agaaaaaact
    34261 cgaaggcgat gaagaatagg gtttacgcct gatctttcaa tgcgcgcagt ttggcaaaat
    34321 cttttgccga tccggcgcgt aaaaacacat tggtctcttt ttcagttttt aataaaaccg
    34381 gcagcgttga ttttccttgt ttacgcaagg cacggacctg tttgacgcgc tcttgcagtt
    34441 ttgcattatc tggcgcaatc cttaaacaaa attccgcatt ggccagcgta tattcatgac
    34501 cgcagtaaac ttgcgtatca tcgggaagtg cagcaatctt ttgtaacgaa tgccacattt
    34561 gttcgggcgt gccctcaaac agccgcccac accccatgga aaatagcgta tcacctgtaa
    34621 acaccagctt gttctcagga aaatacaggc aaatagcgcc gcgtgtatgt cccggtgttt
    34681 cgagcacatg caatgcttca ccgccaaatt taaaattgct atcctcatcc agaaaaatat
    34741 ccatctgggg aatgcgggcg gtttcactgg ccggtccggc aaccgggcag ggatgccatt
    34801 tgagcaggcc ggaggtcccg tcaatatgat cccaatgatg atgcgtgatc aaaatcacat
    34861 ctggtttgag acccttcgcc tcaagtgctg caatcactgg cccagcctcg ccgggatcga
    34921 caatcgcaac gctgccgtca tcgccttcta gcaaatagca gtaattatcc ttatgaaccg
    34981 gaatgagtgt gacgttgatc ataggggctc tcccgctttt tcttctctgg tcatgagcgc
    35041 atcagccagc cgtctttccg cactccccgg tttgagaggc tgttgctggc tttcatgcgg
    35101 tgcccagccg atcagatata taatctcaaa gctcgccgtg atccgtccgt ctttttccgt
    35161 aaaatgttcc tgataatact gtcccgcgcg cgtgaaaaac gctttgcctg gattaatgcg
    35221 gctgcgcgcc gcaatcacat tgctctcccc catgccgcgc agatcatgca tgagcttaaa
    35281 tatgtcctca taactcactc gcacaatatc cgaatccacc accggcaggg caaaccccgc
    35341 gcgctgaagc agcccgccca tctgctgctt atccgcaaat ggaaaaaccc gtgggcttgc
    35401 gccgcccttt aaggtcaatt ctgtttccat caggctttcg cgcaactcat aaagcgtttc
    35461 accaccgaac atcgccgcca taaacaaccc gtcgggcttg aggctcttgc gaatctgcac
    35521 tagcaggccc ggcaaatcat tgaccgcatg caggttcaaa caactggtga tcaacgcaag
    35581 gctttcaggt tcaaaggcca gcgcttcagt ctcattgtca aagtcgtgcg cagacaaatt
    35641 atggcgcgca ccaaagtcgc gccgaacatc gcccagccga tcgagaattt gcccccaggc
    35701 catatcatgt aaaaaccgat attgatcaaa gctcgaagca gcgcgccggc gcgctacgcg
    35761 tcgcgcatca gggtcaaaca gagtggtatt gtcattcggc ttcaccattt ttctgggcgc
    35821 ggcgtgaggt caggttcgtt ttctaccaca tccataaacg cctctaactc cgcctcaaat
    35881 tctttttcct gcgctggaga aatctcctct gcagcgtaat taggctcatc agcttgaggt
    35941 ttggggtctt ggggtttttc ttcaatgggc atcggcgtgt tttgctgatg gaaggccttg
    36001 tgctgtgctt tctttacatc ctcttttttc tcttccagcc gttctttgag ctgcacttcg
    36061 gcctgatatt tcagcttttc ctgcgtttgc gctttcagca cttccaaaga attataattc
    36121 agcggaatcg accagtccgg ctcttgcccg gcaaaacgct gctgtaaaat cgctttgacc
    36181 tgccgcttgg catccagacg catttcctca tgaaaatacc ggtaggcccg tgccacagcc
    36241 agcatgaaat gcggttcttt cgagagtaga cgctggagca gcatcaattg ataaaacggc
    36301 aaaaacttgg tgttcggccc cttcatcgct tcggcagggg cggccacaac aaacggatga
    36361 atgcggtgtg gtgtcatgcc gacatgggta aaataactct cccccagctt aataatcttg
    36421 ttcggcgtta cgccaaactc atcagccaga aacttcttca tcattttgag gttggtcact
    36481 tcggccggca cgttaaaact cggcaccgaa attgttagcc cgttgccctc atgccgctgg
    36541 ggtacgggta aatgatgggt aaccacgcct gcatgcacgg tgtttttttc atctttcgtg
    36601 agcggcagca caacagccgt attgattgtc ttaccgtcat gaacgatgaa atcaacgctt
    36661 tcagccgata gccccgatat gctcccgcgg ctttgcccct cttcaacaaa ggtggaatgc
    36721 acggaacgca attgtccggt ggtgccttta acctttttaa agcgaaaatc ttcttcgccc
    36781 atttttgtca gaagattttt tacatcagct tttttattga ttttcgaaac ctgtaaattg
    36841 agttgccttg atgtccacgt ttctgcgcgc agtctcagat gatgaaacaa agacagaatt
    36901 tgcatctcca gccgcgcatt cggaatcatt cccaccgtaa tagcgttcag aacctgctgc
    36961 gcatccattt cgcgcaccac gcctttggcc tgaaattttt ctacgccgat gacctttttc
    37021 ggcgtcaccg catttttgct agggcatacc cgcaggtaaa aagtgtgaat gcgttcatca
    37081 atataatcgg gcgcagggta ataatcaggc ccatgttcga taatcgcatt agcctcaggt
    37141 tttaagccca gataatcacg cgcgaaaaga acgctatgct taacatcaaa cgtttcgatt
    37201 gatgaaatcg cttctccatc gacagtaatg gcttccgcca tatgcccgga ccagcgccgc
    37261 tcatcaatat tcactccgcc gcgcggcacg gcgttggtga tagagcgcgc caccccgtcg
    37321 tgcaggtaaa ttttcagccg cccttcttca tcaacgcggt aaggaatgac ctcgctcaca
    37381 tcaatatccc ggctcacgac atccatgatt tcaccggttt tctcgttgcg catggcggtg
    37441 attttgagct ttccagaatc cgttgaagac ggacggcgct cctcaatatg caggctctta
    37501 cgttctgcca tcttataagc cacagcaaca taacaagttg gcggatcacc caaaggcgta
    37561 ccatcatccg catataggcg aaaacgccct tcaaactttt ccttaatcag atcatcatcc
    37621 caatgtgggc cggaatattg cacccgcgcg ccgagtgcac tgagttcttt acgaaattcc
    37681 attggcgtaa agaacgtgta ttcttttggc agctcacttt cccagtgaga gcgatcatcc
    37741 ttgcgcataa taaattcata ggcccattta tacggcaggc gaaatagccg cgtacgtgga
    37801 aaccgctcag gcagttcttc aagaaaaaac ccgccgcagc ccgcatcttg acggggacgg
    37861 gcatgttctg cataccatac cagaatatcg gggggagaaa gacgcgcaag ggtcttgccc
    37921 gtgcttggct catcaggcat ttcaagcaga acaaattctt ccggcggtgg tttcgcaaaa
    37981 tcgcgaataa acatcacgcc gccttttttc agcatcttga attgcttacg cagcgtatcg
    38041 ctaacagttt gttcgctata acgcgaattc gaaaaaatct cgtgaaggat ataagaattg
    38101 ataaccgcat caacggattc aggctcaaaa atctccgaag aggcatcccc aactttaaaa
    38161 tcgagattaa acagtttata gatctctttg gctttgttca cctgacgttt ggccttgtcc
    38221 agtcctgtga agtgcacctt cggattcatt gccgccatag cataggtcat tgccccatca
    38281 tcgcagccca tatccaccac atgtgcccct tcatccagta acaagtgcga cagtgtaaac
    38341 catgctttac tgatgacggc aggctcaata tctttcagca gcaagttgcg cttctcgata
    38401 taactttccg cttctgtttc cagtttttct gccatagcga cgcaccatag cgattttacg
    38461 caaaatattc aaatatataa gcgttaaaca gcgctttttt cgtcattgcg agccgaaggc
    38521 gaagctatct atctatctgc cattggattg ccgcgtcaga tctgacggcc ttcctcgcaa
    38581 tgatgccccc aaaaaaaccg tgttcatctg cattgatctg tggtttttta aatcttatgg
    38641 atgcagcctt taaacccttt caagacatgc tcatgcaagc ggtagacacc gttttgccgc
    38701 cgcgttgcgt ggtcacgggc gaaacggtcg atcagcaagg tatgctcagc cctcaggcct
    38761 ggaatacgct tgaatttatc accgatcccg tatgcgctca gtgcggcttt ccttttgatt
    38821 ttgaagtcga ggaaggctcg ctgtgcgcct catgccttga tcgcccgcca ccttttgatc
    38881 ttgcccgttc ggctttgaaa tacaacgata cttcacgtga tctggttttg ggctttaaac
    38941 atggcgatca aacccatatt gcgcgggctt ttgtgctatg gctggaaaag gccggggcgc
    39001 gaatactgga agaagccgat atactaattc ccgtgccgtt acaccgctgg cggcttttat
    39061 cccggcgtta taatcagtcc gcaattatgg cctgtgcgct gtcaaaggcg gtcaatattc
    39121 ctgtggaata tcatgctctg cgccgcaccc gcgctacgcc cgtgcagggg catctgaagg
    39181 ccgctgaacg tcacaaaaac gtcaagcgcg cctttgcgct ctgcccggat cgtgaaaaaa
    39241 tcatccacgg aaaaacggtt gtactgatag acgacgtatt caccacgggc gcgaccgtga
    39301 aggaatgcac gaaaaccctg ctcaaatccg gcgcagccaa ggtgcatgtg ctgacattga
    39361 cccgcgttgt acgtgaaggt tttggatagg ccataattat ccatttgcct tggcatgggc
    39421 gctgaactac tttttaattc gtcatcccga ggagtttacg acgagggatc tcatacaaca
    39481 tgttctcgac cttgcggcgc tgtttgtaga gcgcttcgtc atatgcgctt ttgattttat
    39541 ggttcttctt cggcgggatg cgcggctcaa tgcttttctc acacaattct gttatggatc
    39601 ccaagcttag aagacatcaa gcgctgtacg taaaacaacg aaattgccgc ttgtataaaa
    39661 gagatgattc gttaaattga tccacggtaa gtgggctcca agcccttcac caggtatatt
    39721 atataggttc tttgtgaacg taacgcacat tgatgttgat gctggccaga tggaagacaa
    39781 ctataattta aaattagttt catttgtcct agtcataatt ataggcttga tgatgtgcct
    39841 ttattccctt gttgccggtt attgcagaag gaaaaggggg gacgaatata ctttgttaca
    39901 ccaacattac ttagaggtgg tctcggttct tcggccagct ttgcggcgaa gttaagctac
    39961 gatcg
//
LOCUS       DAOSWR010000004        36695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_742_length_36695_cov_5.640311, whole genome shotgun sequence.
ACCESSION   DAOSWR010000004 DAOSWR010000000
VERSION     DAOSWR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 36695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36695
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_36695_cov_5.640311"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK513_00595"
     CDS             complement(<1..749)
                     /locus_tag="PK513_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOO80986.1"
                     /translation="MFFTFTGWSGLALAAGDACEKARSSADVMKCLTRQLDVAQETLN
                     SVFDDLSMQNSGEDLVEIKDIQADWLSYRERECALEIAGLETESLKRLENLRCLNRLT
                     QARIEALRRSLNTQDDFEILGEAAASPRWMNALANDHPDVFWAYGAQIEGDLDCDGEN
                     EHIMSGLRSSEDFMEVFPVVSISESPLTGRPKSTVIGLGFDDESDNVDEERVRCGFVV
                     GLSFAQPPDEAQLPEDKGGEGQVSDAGASSAA"
     gene            complement(848..1984)
                     /locus_tag="PK513_00600"
     CDS             complement(848..1984)
                     /locus_tag="PK513_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80987.1"
                     /translation="MSITGPNGYNADSFQLKGTFGWNPDQRTVMDVTGSALDASFSGG
                     PAQTALTQFTGQNYLKDMPMAVNKAGQNVSANMVSATGAAVDLGQVENRIGVVEQAVT
                     NMQQPFRQAFDVGAEKLGFDRASVGASFEPGPATQMIDVLADAAGMKAGIPAAKAVTT
                     AMNVADAVSSAKTIVGKLSPKNEAKIADMARTLLTPKIDPMTGKEIGAPAIPNSLNTQ
                     SLVSMKPHELVDTMKQIMLPPSEHPEMKQLLALKSELSMDVGVHEGQRNTNEAALAMA
                     EEAGFSLEDLTIDEADIVLNVAEAQDFKDQAAGVQGFLNDGFAGMVSQGEIQVSPEMA
                     AYALKAGGMSSEREKPSIGQDREDELVRLAQAAAQYDSKPMMGA"
     gene            complement(2178..3707)
                     /locus_tag="PK513_00605"
     CDS             complement(2178..3707)
                     /locus_tag="PK513_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOO80988.1"
                     /translation="MVARVNTVAFQGIDVQPVDVQVQISNGLPAFTIVGLPDKAVAES
                     KERVRAALHALGIALPPKRLTVNMAPADLAKEGSHFDLPIALGLLAAMEIIPKEELAE
                     FLVLGELSLDASIRSVTGVLPAAMHAAGNDNKLICPSSCGGEAAWVGNLDILAPKDLL
                     QLINHIKGTQVMGQPEARLTSDGSCAGPDLADVKGQETAKRALEITAAGGHNLLMIGP
                     PGSGKSMLASCLPGILPPLSPREALEVSMIHSLAGSLPDGGLLQQRPFRDPHHSASLP
                     ALIGGGQRVKPGEISLAHNGVLFLDELPEFSRGTLEALRQPLETNEALVARANAHVRY
                     PAKFQLVAAMNPCRCGHLGDPDLECTKAPRCASDYQAKISGPMLDRMDLHVDVSAVTL
                     SDLNASGGERSETVAARVAAAQELQHQRFQALGVEGTLNAYADGAVLEKIAVLSPEAK
                     VLLDKAMETSKLSARGYYRILRVSRTIADLSAVDFGQAFENIEKIHVAEALSYRRAVL
                     V"
     gene            3905..4483
                     /locus_tag="PK513_00610"
     CDS             3905..4483
                     /locus_tag="PK513_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80989.1"
                     /translation="MLSEKELAKLTNTLQIPMIVQGILDGQESFTPDMQYGLHEILSD
                     YQPDSALLSIALGAHKIAAHFQNHSPNMAILKLECERIISDYAEIWVRNAESKTLDDN
                     LVFDTLEQIPEDLETLAELLEINATLLNAHNEELAKICETFALQAHAQVLIADTFIDL
                     MDEGAELAETYAPTTTIYNDNVIPFPGTMNAS"
     gene            complement(4514..6655)
                     /locus_tag="PK513_00615"
     CDS             complement(4514..6655)
                     /locus_tag="PK513_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80990.1"
                     /translation="MSASVESVSLPHILARIVDNPEMQRRWKQHYITRKTDNPGSKAA
                     WELLGLGKDSEVWADKFCQHPQLLRFTREVIEIERGAARSRLRNPLAQQDSELIQKRA
                     GLDQLAGYLSSILPEEEVPPVKYLSEDQQRYLKMLERETAQPMAQPIRRSAQSTARFS
                     AHMWEEIKDHKMALTLSAPVAAYMTWLTFNGKLNVGATKLSVEEAAATGMDATMFLPG
                     GGQPKEIKKDYSLVDPNYTGGCHPHNLVKMIAPDFVADAALSVGLDKVLSQESCMTIN
                     KAALATHGVLNDYREYAVNKGYVNMGKGVDVSMIIKPGTPAADTFVKAIETSQDQVAA
                     VAIGENFALHAAIPSVTYGEGLTAVLAMFAASAVYKKSKIRKAWGRATRPVKELFRQE
                     TELMSFGMRQAVKPVTQRISYGAQSIKEFMAETAVCYAPGLTVNFCRFAEKRSIKKAR
                     IAAFKSYAEHKDDQKSFWKSVGDFAVRSSRKNFLGWAGFIAGAAHVAQESDYIFTLAK
                     GLNAEAAWMGIGGMVGGNTVHKLHKYFTRKAHFERATEGVGEELKAFAFPENTMLDQA
                     GYNIPKLQRKKTDIPLTLVGSGVVTAVSAVDFYCTGGHNFATVAGHAVTWGPWLAINP
                     IEDTTVHGFLGASGYALGAGTGATVALSFGTAEGVYRVGEVFYKAGTGVINQKIQIEP
                     KGNVVLLQDRPRKVETPLPVVDAKNQTGLHL"
     gene            complement(6668..7141)
                     /locus_tag="PK513_00620"
     CDS             complement(6668..7141)
                     /locus_tag="PK513_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80991.1"
                     /translation="MSLRDCFGGALLASLSVLPTFQAQADPIIEHPHGAYTINGKKRL
                     PVDCGEYTRYLDDSIYVSCEAGRQADPNNEKKCTDDPDLTNVSDADKRKSLESSLKEY
                     TIHLARVHGECVPSYTANINSNLRNIPPHYRDNTQCVFTEVLKGCMDAGFQIPRR"
     gene            7305..7700
                     /locus_tag="PK513_00625"
     CDS             7305..7700
                     /locus_tag="PK513_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80992.1"
                     /translation="MEKDKKEKGMLSKVFGGAAKLATIGVAAAVTWQILLDPIFFPII
                     HNLAAHPTAAAWVSFIQDHTAFITDFIGFTGDGGLLQSEWGDALLADYYPDPVSSGTT
                     AVQQGTSVATAVPTPAPKMDATMFLPQPG"
     gene            complement(7708..8073)
                     /locus_tag="PK513_00630"
     CDS             complement(7708..8073)
                     /locus_tag="PK513_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOO80993.1"
                     /translation="MNYDNQNIFAKILRGEIPCDKVYEDEYALAFKDIDPKAPVHILI
                     IPTGRYVSIDDFGKRASVEEVAGFFAAVAKIVNEQGLNKNGFRSIANTGVDGGQEVPH
                     FHLHILGGGPVGPMVSKAG"
     gene            8120..8473
                     /locus_tag="PK513_00635"
     CDS             8120..8473
                     /locus_tag="PK513_00635"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOO80994.1"
                     /translation="MLLRMLKSKLHRATVTQSDLHYEGSITIDPELMNVGEINPYEQV
                     DVLNINNGERFTTYAIEGQRGSGVIGINGAAARLVQAGDLVIICSYCEIEKEKARGHN
                     PTIILLDDNNMPKTT"
     gene            complement(8474..8674)
                     /locus_tag="PK513_00640"
     CDS             complement(8474..8674)
                     /locus_tag="PK513_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80995.1"
                     /translation="MKIKTLSVMFTGMLLSAACATGPEAGRSGAPVVPSDNKPTQQGP
                     PSKYQDTTWGEGAWLTDNRCIV"
     gene            complement(8724..9068)
                     /locus_tag="PK513_00645"
     CDS             complement(8724..9068)
                     /locus_tag="PK513_00645"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOO80996.1"
                     /translation="MSAHILEKLYAVLQERKKADPESSYVASLYDKGSVKISEKIREE
                     AEETIVEGLRLEKSSGDEAIRQALTNESADLVFHLLVLLSYHNIAPEDVFAVLERRFG
                     ISGHEEKASRGH"
     gene            complement(9086..9850)
                     /gene="hisF"
                     /locus_tag="PK513_00650"
     CDS             complement(9086..9850)
                     /gene="hisF"
                     /locus_tag="PK513_00650"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOO80997.1"
                     /translation="MLKTRIIPCLDIDQGRVVKGVNFVDIVDAGDPVEQAKIYNEQGA
                     DELCFLDITATHEGRDTIIHMVEHVANEVFMPMTVGGGISKILHIRNLLSAGADKVSI
                     NSAAVKNPDFVRAAAEKCGSQAIVVAIDAKEKEDGSEGWEIFTHGGRKGTGIDAVEWA
                     VKMAEYGAGEILLTSMDRDGTKIGFNLELTRAVSDAVPIPVIASGGVGNLQHLVDGVK
                     EGHASAVLAASIFHFGEYTIAQAKQAMAEAGIGVRL"
     gene            complement(9896..10210)
                     /locus_tag="PK513_00655"
     CDS             complement(9896..10210)
                     /locus_tag="PK513_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO80998.1"
                     /translation="MSGKYTSVFDAFLAEVLGGASCEEKLAFMAAFHKAQEAMNLKDG
                     LEQNHLKNPNIALALTRNSGLAAFEGFSQAVAGIAVAPTVKENGLTYIVVEDTAPKVP
                     MS"
     gene            complement(10275..11003)
                     /gene="hisA"
                     /locus_tag="PK513_00660"
     CDS             complement(10275..11003)
                     /gene="hisA"
                     /locus_tag="PK513_00660"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOO80999.1"
                     /translation="MIIYPAIDLKGGKCVRLVQGDMTRDTVYNEDPVGQALEWAQTGF
                     SWLHIVDLDGAIEGVPKNHCAVRAILEMVDIPVQLGGGIRNRGQIEHWIEAGVSRVIL
                     GTAAVRDPELVKWACRKFPGQIAVGIDALDGEVMVDGWIDGSNIQASELAAHFEDAGV
                     AAIIYTDIDRDGTGKGVNVEATCALAESTSIPVIASGGVGSIADIYAVNEASKTHGLN
                     GVVVGKALYDKRVDPEEALAVLSS"
     gene            11145..12221
                     /locus_tag="PK513_00665"
     CDS             11145..12221
                     /locus_tag="PK513_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81000.1"
                     /translation="MSNSRLSTLVFDDAKKKVHNASLETLQPVIGRLEETYKEKPKAT
                     ATLINILLEHPSYKVVCALAKSKTLSHFPEAQMNLATHERLKHKSFLAALPGLTDDVQ
                     VHLSRLGFPEAAPSTNSKSNTLALENNNEIVLETGKNSSKTANFNTASHDHNNGYKFE
                     MLPLITQEDKNRRTNAQYIKATKIALAQNPYANSKEAQLNLATNPDEDIQLVLAGRKS
                     PIDEDAQIELSKSPFPSVQALIAGNINLTSQQAQLNLSKSDRQDITAPLAGNISLTNE
                     DAQHNLSKSPWAKTRIALVNNDSLTDPDAQNNLAIDSDESVRSAVINRTKPYKSGHYL
                     GHLHGFAKNYQEYKLKDTLSDLNI"
     gene            12384..12938
                     /locus_tag="PK513_00670"
     CDS             12384..12938
                     /locus_tag="PK513_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81001.1"
                     /translation="MKLQRYNIALLPSDHTLQTTMVHISRLYFLEDQDEYILGPEGLP
                     HITLCQFKAATLENAVTAYNDFLSNESISTIKVIIEKFNIRPGKLVNTGKFIAEYKIQ
                     PHPDLIALQLRCAKKLAAHHLENLTPSEAYSPHITLARLPVMTNKLPTKQDLNCPLYI
                     DAHLSLGLSTEAGVFVKELSAHTL"
     gene            complement(12921..13718)
                     /locus_tag="PK513_00675"
     CDS             complement(12921..13718)
                     /locus_tag="PK513_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81002.1"
                     /translation="MKSNALRGDFSLANIAPTLEQLMSWKEQGFKKIVLFGRLQPPHR
                     GHRALMETLRHSGLDVNLVLNDKTDSVEGDRNPFNPHQRDEMARISMPWLPAENIRHA
                     QVYLGAGGDVGDAVRRLTGIFNSIALPDKLVFAYFEKDEDRKDYLVDGKNIKGVHYVE
                     LVGQPRGLFPIQRITQEMIDAVSEYQPIDAKMFREGVREQDDICYELLDPPVAEYIKE
                     QMELAERNGRLVGDDPSGDKVTLEDLRRENGTAPRESTFELVLEGVS"
     gene            complement(13827..14399)
                     /locus_tag="PK513_00680"
     CDS             complement(13827..14399)
                     /locus_tag="PK513_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81003.1"
                     /translation="MSDQTKLKPLAPVPTVEVIESLGTADLNDLCDATDAAIEGGGGF
                     GWVHLPARDMMERYWQGVLAMPARILLAARLDGVICGTCQLWKPPAHNEAQAHIVTLT
                     TNFVAPWARGHGLAKMLVEKAEEVARAEGFAVINLEVRETMSRAIEIYEALGYVRFGM
                     HPYSVRLPDQILKGYYYYKVIDPSVVEQQS"
     gene            complement(14425..15111)
                     /gene="hisH"
                     /locus_tag="PK513_00685"
     CDS             complement(14425..15111)
                     /gene="hisH"
                     /locus_tag="PK513_00685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOO81004.1"
                     /translation="MSKETIVIIDYGSGNLRSAAKAFEHVIANEAIDAEVQVSGRAED
                     VLRADRVVLPGQGAFGDCMNNLNSTEGIAEALEQRVQKDGKPFLGICVGMQLLATRGL
                     EHGVHAGLNWVPGQVVPLAERINDPALKIPHMGWNQLMLPHSGEQDNRHFVLRSTESS
                     SQGGDHYYFVHSFMFECEYNHHVLGLVDYGGLFPAVVGRDNVVGVQFHPEKSQSAGLR
                     MISDFIGWNP"
     gene            15172..16497
                     /gene="hisD"
                     /locus_tag="PK513_00690"
     CDS             15172..16497
                     /gene="hisD"
                     /locus_tag="PK513_00690"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOO81005.1"
                     /translation="MSINFYRWNEIDETTKAGIMRRSQADIDDMIAIVEPIIKDVKAR
                     GDTALIDYAKKFDKAEITNLKSTAEEFAAAENALDEDLKAAIAHGIRNVQKFHEEQMR
                     RAEKDRSWMVEIEPGVFAGEQINAIESVGLYVPRGKGAFPSALYMLAIPAVIAGVKNV
                     AIVSPPTPEGTTDAATLYTAKLCGIKNVYKCGGAQAIAALAYGTQTIPQVKKVLGPGS
                     PYVAAAKRVCSVIMDPGMPAGPSESIVLADETADPENTCWDILNEGEHGEDSAALLVT
                     HDEKLAKFVQQNLPSRIAELPEPHKTICTKVMGQDGNERGYGGIILTENLSQSIEIAN
                     TYAPEHLLLKIADPDSALHRLTHAGEILIGEYTAFSLGNYATGVNHVLPTGGWAHTYS
                     CTSVWDFLKRTSLSKCTHEGFEAIKNSVITLTDYEGFPAHGDALRKRKI"
     gene            16499..16984
                     /gene="greB"
                     /locus_tag="PK513_00695"
     CDS             16499..16984
                     /gene="greB"
                     /locus_tag="PK513_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOO81006.1"
                     /translation="MSEDIHYMTPAGYKALADEQDHLIHTERPKICEIVSWAAGNGDR
                     SENGDYIYNKKRLREIDRRLRYLKKKLDNCKIVDPKDQQNLTRVYFGATVTYVREDDS
                     KITVHLVGVDEADFSQNKINWLSPVGKALLKHEVGDSVKVRVDGQIEEIEILEIKYMI
                     D"
     gene            complement(16981..18105)
                     /locus_tag="PK513_00700"
     CDS             complement(16981..18105)
                     /locus_tag="PK513_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO81007.1"
                     /translation="MNPFSFGNPVSDEYYLPRPELNSLVRTYLDNRIHVVLMGPRRFG
                     KTSFVQNLLRGFEDTHVCLFMDIFNITSHRDFLQQTLRALRAQSTWGQRFKEWAETIP
                     HLRPRIGWDVSESGQYSFSLSADISDDRDVKETIQDVLSGISSLGERVVFVIDEFQQM
                     AELDDNGWLEATLRTKMQQLGNTVFLFTGSRQSLIHDMLNDQARPFYRSCQQLDFPVF
                     GDEFIDWIIERFASVDVECDRDAVIFLRDEVQNTPNYIQMACFHLVAQGERYIGMGEV
                     KKVLNTIVGQNSYAYQTLLGSLTLMQQRILRLAAIEREQIFLKENLDKYEVTSGAALA
                     SAINALKNKYILDEGSGKGRVIFDDPLFAIWLRKEFSSDI"
     gene            complement(18202..18810)
                     /gene="hisB"
                     /locus_tag="PK513_00705"
     CDS             complement(18202..18810)
                     /gene="hisB"
                     /locus_tag="PK513_00705"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOO81008.1"
                     /translation="MPKDGAKERLGTITRKTKETSVSVTVNLDGRGVYDVSTGIGFFD
                     HMLEQLSKHSLVDLTIACEGDLHIDGHHTAEDVGITLGQAIKEAVGDKKGIRRYGHFD
                     LVMDEARSSVALDLSNRPYLVWDVEFTVERLGEMDTELFEEFFRALVGAAGITVHVSQ
                     LAGTNNHHIIEASFKAFAKALRMALETDPRAADALPSTKGAL"
     gene            18875..20272
                     /locus_tag="PK513_00710"
     CDS             18875..20272
                     /locus_tag="PK513_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOO81009.1"
                     /translation="MKFPYKRLLQTITAVALLTGCGGSIVKEVDYAPTPDIPETAHPT
                     PIKYSGIELLLPPGMNIGEARHGIFCNSPAYPVNRNVIAKEIDHKFLRQGFHDAMEAN
                     GYDVVGSLDIAFDPDDEEQRAEYSIKGKLKDAQLDMCTSQEGALNWATPVPGEHGRMY
                     IAIDWSVYDVLHRTIVYKTRTEGYSNRNIPNTEGMTLLFQDAFEMAVHNLAANEPFRA
                     LVVDGVKPPQKWPGKDMRGEKTDSRPRLFDEDEDVTLSDQPLSRQPFAKTAEDGRKTA
                     VLIEKDGHGSGFFITKQGHILTNAHVVGESRRTRIVTAARKTGITAEILRVDRVRDVA
                     LLKLEEIPEWLDIQTLPVRLDWPKVGEDIYAIGSPKDWKAFKGTISKGIVSAHRRNMK
                     YGGPRSNYIQGDIETHPGSSGGPLLDEYGNIVGLSVKGEPFANENGARIGIGLNLFIP
                     IREALDRLGIAYEGQ"
     gene            20727..21224
                     /locus_tag="PK513_00715"
     CDS             20727..21224
                     /locus_tag="PK513_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81010.1"
                     /translation="MALPKFNKETFRKSVLGAGGTIALALSVSPANAENDICLPLTQI
                     NGITLSADATADRHSRSNPGVISIATLPGGDMRNSPENLTKVFRKKGIGADCFLNNSM
                     ASNYGTEFSLYVAGNPVKFNGKTSFGIQELRDNPEILRTAHAEAIVSKQMLAGTVNTP
                     RLAGQ"
     gene            complement(21232..22032)
                     /locus_tag="PK513_00720"
     CDS             complement(21232..22032)
                     /locus_tag="PK513_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81011.1"
                     /translation="MAITRSQFRENGSDLTTRAEESLRANFATQADPIEEALGLGGEF
                     EKASRQNFYASGSEESFKLHQDEIAVGQGQLERGQVNAQINEARAGIEALRAAQDEKS
                     AQRHGDIMLLMLLDQLHAMEDALAEQYGESFAEDLLSDLQEQGLVDQDEYDRIMGIHD
                     QDERRQAIAKLIQEKLDSGELQSSNLQGHPWAQEWLQQHNKVQQQIQAVAENAVETGR
                     SLESLDKRVINRAEEIKAQQGIEIDRPTVEQKLQFAEANSSPDDGFGY"
     gene            22440..23003
                     /gene="hslV"
                     /locus_tag="PK513_00725"
     CDS             22440..23003
                     /gene="hslV"
                     /locus_tag="PK513_00725"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOO81012.1"
                     /translation="MNNEHQNYANAQSWRGTTILSVRKDGKVVIAGDGQVSMGSTVLK
                     NNARKVRRLGRDDNIIAGFAGSTADAFTLFERLEAKLEAHPGQLTRACVELAKDWRTD
                     KYLRRLEALMAVADKDTSLILTGNGDVVEPQDGIIGIGSGGNYALAAARALYDQKLNA
                     EQIVRKSMQIAADICVYTNENIVVEEL"
     gene            23008..24351
                     /gene="hslU"
                     /locus_tag="PK513_00730"
     CDS             23008..24351
                     /gene="hslU"
                     /locus_tag="PK513_00730"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOO81013.1"
                     /translation="MALKSEKTDAIEIPAFSPREIVSELDRFIIGQSDAKKAVAIALR
                     NRWRRRQLEPALQEEVYPKNILMIGPTGVGKTEIARRLAKLAQAPFVKVEATKFTEVG
                     YVGKDVEQIIRDLVENAIHMTRDSLRKTVQAQAEIYAEERVLDALVGPTAGEGTRNSF
                     RDKLRNGELDEREIEIEVIDNASPMGGMMDIPGMPGASMSMINMSDLFGKAFERTKKK
                     KMRVDESHEILMREESDKLLDEDKITAQAIELAEQNGIVFLDEIDKIAARQDVHGGDV
                     SREGVQRDLLPLIEGTTVTTKYGPVKTDHILFISSGAFHYAKPSDLLAELQGRLPIRV
                     ELRALTQDDFKRILKDTEASLITQYVALIGTEKVTLEFEDEAINEISRLSFEVNENVE
                     NIGARRLHTVMEKLLEDISFTASDRGGETITITADYVRKTVEELVKSADLSKFIL"
     gene            complement(24348..24941)
                     /locus_tag="PK513_00735"
     CDS             complement(24348..24941)
                     /locus_tag="PK513_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81014.1"
                     /translation="MGKTALFFEAALARKAPVMLTLPGDLSMFFGKMAKKGFKHSEIP
                     QTQKWINVRYRFQATKDKAYGRSVYCFLEPVSLLAFENAARGALGVGSRLFVSFADHY
                     IPFGDFKMRSQSLEQVEVGLAENDPKIVLYQHGDYRIGMPRLRKDDMRRSVPFIPYIQ
                     EEQGSVEWLPITEILGEEQVAPSVDSTGRFEPQRVAF"
     gene            25052..26035
                     /locus_tag="PK513_00740"
     CDS             25052..26035
                     /locus_tag="PK513_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO81015.1"
                     /translation="MQKLLIICAILASTLSSAKAQNTPKFSFPLDCTLGTDCWIANYV
                     DVDPTEGVKDFKCTAKTYDGHKGVDFALKSRVIMNKGVNVLAAADGTILRMRDSENDN
                     PKTKQEYDAITAANKDCGNGVILDHGQGLQTFYCHLKNGSIKAAPGQKINAGETLGQV
                     GQSGFAEFPHLHFTVIWEGSHIDPFTGMTKEDGCGKFKQSLWNKEIPYAPYALFNGGF
                     AENVPDFKTLNETGTRPQEPTLTSKAFVFWISLYQMQKGDQITLKVLDPDGKTFIHRE
                     ITQDTNRARQHYYTGRKLKDHSLKPGTYTATATLKTEGYETYTQDFKTTIK"
     gene            26044..27351
                     /locus_tag="PK513_00745"
     CDS             26044..27351
                     /locus_tag="PK513_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81016.1"
                     /translation="MKTTTEQASMQSLSRDYSQDFRPSDVRDNEFQELTVARHAHGRL
                     LLANPCSQLRQDTHHIRSMVLSQKRKGKEANAHQAVHEERPDGCIRLDDQEIDRYDKT
                     SLCFIYVPETEFKKPPSEIKGLGTLRIIPSYSGQLLHAHNVTKKNRPEDLPTAPTYTN
                     QRDPDPLASWIKNAGDRSLSSRTNPFIVEFSRLHISKQRRVETGYPSGISPTEFILAA
                     AIRLTLDMHRVALPTYPGACLEHSVVEPALIKMRKRMNQHPESQGETFEYLGRKRFAV
                     YNVWGCYQDIRKNRPEIFEQMSCFNSEPLFEYLNFGNSLCAAWTNERLKQTEHMLTDE
                     TSVHKLQNRNGKIEAFALRDANDNNRLLAVLFLNAAGNGVQTLWDIDTEKIYINRQAL
                     PVETVETAKNTPYLKALYDDLLKRGTWTESPPIQKYDSNDMAL"
     gene            27335..28123
                     /locus_tag="PK513_00750"
     CDS             27335..28123
                     /locus_tag="PK513_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO81017.1"
                     /translation="MTWHYNTAAGILDYRTLEKPLIQTSRKYMSVTPLDDVTSWLKYR
                     RVIAQPEQHDQSFSKMWFLFRITDFEKNKVYCRLGDKFIHVYSPSFFFIPAFMHAKWH
                     FQPGIYEYEAYTSYADVHDELPARPIFFPYDRYQDIQSYSAVVNLLNGINGPEISLRR
                     KQSYAAEKTKLFLDLYYTEDVGFDDIAKELKLPRSTMSRSFQKFYRITPIAYRNILRT
                     WEAMRLIRNDISVTQAAMDAGFTSLTQYNQHFHHVFDAPPSSFS"
     assembly_gap    28279..28378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28447..29514
                     /locus_tag="PK513_00755"
     CDS             28447..29514
                     /locus_tag="PK513_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO81018.1"
                     /translation="MNVSKTKEIANSAQTRDEDLHNLTMIFGGHIYFQTLYAAIKLDL
                     FSMLADKGPMSQEEIAEALSIESKPARILLLGLSSIRLLKKDGDQYSNMAISDELLCK
                     KSPRNLNKIIEWEHEIVYQGMFYFYESIKANKNLGLEVFKGNEKTLYERLAHVEEKNQ
                     IFQDAMHQISVQANADLVQHVDFSNIGYLVDVGGGDGTNLMQIADSFPHIRGAVFDLS
                     SVCPITEKNLKDKGYSNRIEAISGDCFQDAFPEGADCFLFSHFMTIWSEEKDRTLFQK
                     AYDALDDGGKVMIFNQMQDNDRQGPLFASIGSPYFLTIATGEGMLYTWQEYEDWMKDA
                     GFQDIQSYELPRNHGVIIGTK"
     gene            complement(29519..30058)
                     /locus_tag="PK513_00760"
     CDS             complement(29519..30058)
                     /locus_tag="PK513_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOO81019.1"
                     /translation="MVEDDEEDLWDFVTRDVKPIARNKPLKVVVSHKKSAARRVVDSA
                     GQRQVDELPDQISERDHAGVGLDRKTADRLRKGQIPLEGRLDLHGMNQVQAHKALEVF
                     IMQAQREGRRCVLVITGKGAGEDGRRDPLSSGQGVLKRRVPQWLASGSLQGYVLKSVP
                     AQARHGGDGALYVLLKRQR"
     gene            30159..30725
                     /locus_tag="PK513_00765"
     CDS             30159..30725
                     /locus_tag="PK513_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81020.1"
                     /translation="MFSELSKILPIKPRQAEHADTRQDIQRHDPDYERRHSKKDGPEE
                     QEPYETGATVAVEALQHFLEEFLRNNSEEKEQPGPKKETPPLPETQKQNTALPPSPQA
                     NPAARAASAYQTMAIANEKSTVLLETTDQAGGPPLDLSAADIRTIHTLIEDLKNLNDA
                     GVEILHIERAASFLESLVTAVNKIKTTL"
     gene            complement(30733..31341)
                     /locus_tag="PK513_00770"
     CDS             complement(30733..31341)
                     /locus_tag="PK513_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO81021.1"
                     /translation="MAEKLPILIADSQAVSRAAIQRALTCYEGVCVVDVCAPEDVMDN
                     EAVAERCIALGTEAAAILGSRARVHMAKPLRMGALLDRLIRMGKVDTGRGGEDLLHFS
                     GWILDVAHSALRKEGVAEREVRLTEKELSMLRILHEHTDRSVSREVLLDRVWGYVDGV
                     ETHTLETHIYRLRQKIEDDPAMPRILLTTEAGYQLNSGKPAR"
     gene            31479..32285
                     /locus_tag="PK513_00775"
     CDS             31479..32285
                     /locus_tag="PK513_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81022.1"
                     /translation="MIEGLYSALENPASAQTTPPQKVLRMVKNERMGGFVPMWETPKS
                     GESFGEHIEKSLSEALQKTGPSGPENALGYHGMETSAQTSADPSTKEFGFGDIIDMVN
                     PLHHLPVVGHIYRELTGDEIKPIGQIMGGALFGGPLGAASGLVNVVIQQETGKDVAGN
                     AFALVLNGQAPTYRSQTPPADSAPEIRLAAVQKHAQDPHTAQSSDLPGNLLAFVDMKA
                     QPEIVIERTQAASGRTAGSVAKTAYPTLDQNPLPRREPITQIRLSQNYND"
     gene            complement(32350..33033)
                     /locus_tag="PK513_00780"
     CDS             complement(32350..33033)
                     /locus_tag="PK513_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOO81023.1"
                     /translation="MGQVAENLEIINDRIAKAAREAGRSPDDVQLVAVSKVQPEVRID
                     EALAAGQRVFGENRVQEAQARWQGRRAVTPDLKLHLIGPLQTNKVKTAVALFDVIETV
                     DREKLAQALAKEMAKQEDMNKKNLPCFIQVNTGEEEQKAGIVPADLENFYRFCVEVCG
                     LNIVGLMCIPPVDEPAALHFALLKKYADSLGLSQLSMGMSVDFEKAIALGATYVRVGT
                     GVFGERSVR"
     gene            complement(33361..34557)
                     /locus_tag="PK513_00785"
     CDS             complement(33361..34557)
                     /locus_tag="PK513_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOO81024.1"
                     /translation="MKKVLLSTAAIAGMAMIANPAAAQVKLELGGWFKGYGAYVDQDE
                     DSAAANAREVGVIRHTEVHFGGETTLDNGLTVGAHIEAEADGAGGTGDSFDVEESYVY
                     FSGGWGRVNFGAEDGAQYLLQVAAPSADSNVDGIRQFVQPVNYTSMGSAALAAAITAD
                     GLDYETNATGYSDKLTYLSPIMNGFQGGLSWTPDVSGTAVTSHDTGAPDLSGVLTDDV
                     AESFGAAYEAALRYEGQFNNVGVIAGLGYTHIDIEDTTAGLLTGDASDDRTVWNAGID
                     LDIGPFGIGVSYSEDDYGETEVTGGGTRDDEETLVVGVDYTTGPFKLGASYMNQDGTQ
                     NIVGVTGNDGIETDRYTGGVVYTYGPGMTFRGSVSHVEHDNVAGLTSGDEVDATSVLL
                     GTQINF"
     gene            34924..35493
                     /locus_tag="PK513_00790"
     CDS             34924..35493
                     /locus_tag="PK513_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HOO81025.1"
                     /translation="MNATRKTFLSIAIVGMSALTLSACTTGEEEGGAIKTEAKYPTGM
                     ARGANENDIYSKPASIFGEGGLSFGGKRKDNNEDGASGIAVNSFLWRASLDTVSFMPL
                     ASADPFGGVILTDWYTPAETPDERFKLNVFILSRQLRSDGIQVRVFKQVKSKGSWQDV
                     KPSTETARQLEDSILTRARQLRVAQLDSE"
     gene            35525..36355
                     /locus_tag="PK513_00795"
     CDS             35525..36355
                     /locus_tag="PK513_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HOO81026.1"
                     /translation="MSAGVRMPAQGGHDRNKMIPTFIISLSEETARREAIAAHLKDRG
                     FNFEFFDAVDGRQMNVLNHPDYNAPRRRAAHGRDLKPGELGCFLSHRNFYRFMVKNDI
                     DYALLLEDDARLHESTKATLEALIDKDIAFDIIRLLGSPKVAKGKHRKITPLYKDFHL
                     VRLRTAPGGAHATLISRQGAEKLVRATEKFAFPIDTVLGRGWETGITAFSIQPGLAVQ
                     DLSFDSAIGEERHDKSVTPKGLKFKCTRLAFKISEALGKAWVYWSTRASDLAISKKFS
                     "
     gene            36365..>36695
                     /locus_tag="PK513_00800"
     CDS             36365..>36695
                     /locus_tag="PK513_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO81027.1"
                     /translation="MTQEKYNIKESEARWRSVWNERKCFEVTEDPAKDKYYVLEMFPY
                     PSGRIHVGHVRNYTLGDVVARYRRASGYNVLRPMGWDAFGLPAENAAIERGAHPKDWT
                     LSNTSEMR"
BASE COUNT        10317 a        10449 c         7418 g         8411 t
ORIGIN      
        1 gccgcagacg acgcaccggc atccgaaact tgcccctcac cacctttatc ctctggcaac
       61 tgcgcctcat ccggtggttg cgcaaacgaa agcccgacaa caaacccgca acgtacgcgc
      121 tcctcgtcaa cattatcaga ctcatcatca aaccccaacc ctataaccgt gctcttaggc
      181 cgcccggtaa gcggactctc tgaaatagaa acaacgggaa aaacctccat aaaatcttcg
      241 gaagaccgca aaccgctcat aatatgttcg ttctcaccat cacaatccaa atcaccctca
      301 atttgcgcac catacgccca aaacacatcc ggatggtcat tggccaaagc gttcatccag
      361 cgcggagacg cagcagcctc tccaagaatt tcaaaatcat cctgagtatt taagcttctt
      421 cgtaacgcct caatgcgcgc ctgcgtcaac cggttcagac accgcaaatt ttcaagccgt
      481 ttaagagact ccgtttccag accggctatc tccaacgcac attcacgctc gcgataactc
      541 aaccagtcag cttgaatatc tttaatttca accagatcct ctccggaatt ttgcatgctc
      601 aaatcgtcaa acacgctatt caacgtctct tgcgccacat caagctggcg cgtcaaacat
      661 ttcatcacat ccgccgagct acgcgctttt tcacaagcat cacccgccgc cagcgccaat
      721 ccggaccagc cagtgaacgt aaaaaacata aggagaatca aaaatcgaca catacaaaac
      781 ctcacactca aagcgattga gccctgtata tgttataacc aatgaagaaa aaaaactata
      841 gcaggaatta tgcgcccatc atgggcttgc tatcatactg cgccgctgct tgagcaagcc
      901 gcaccagctc atcttcccgg tcctgaccaa tagatggctt ttcccgttca gatgacatac
      961 caccagcttt taacgcatat gccgccattt caggtgaaac ctgaatctcg ccttggctga
     1021 ccatgcctgc aaaaccgtca tttaaaaagc cctgaacacc ggcagcctga tctttaaaat
     1081 cctgtgcctc cgctacattc agaacaatat ccgcctcatc tatcgtcaga tcctctaaag
     1141 aaaaaccagc ctcttcagcc atcgctaagg ccgcttcgtt tgtatttctc tgcccttcat
     1201 gaacaccgac atccatagac aactcacttt tgagggccaa aagctgtttc atttcagggt
     1261 gctctgaagg cggcaacatg atttgcttca tcgtatcaac cagctcatgc ggtttcatag
     1321 agaccaaact ttgtgtattc agggaattag gaatagcggg cgctccgatt tctttacctg
     1381 tcataggatc tattttgggc gtcagcaacg tccgcgccat atctgcaatt tttgcttcat
     1441 tctttggtga caactttccg acaattgtct tggcgctact aacggcatcg gcaacattca
     1501 ttgcggtcgt taccgctttg gctgccggaa taccagcttt cataccagcc gcatcggcaa
     1561 gaacatcaat catttgcgtc gcgggacccg gctcaaaact cgccccgaca gacgcacgat
     1621 caaaaccaag cttttccgca cccacatcaa acgcttgccg aaaaggttgc tgcatattgg
     1681 tcacagcttg ctccacaacg ccaatacggt tttcaacttg tccaagatca accgccgcac
     1741 ctgtagccga taccatgttc gcactaacat tctgcccggc tttattcact gccattggca
     1801 tgtctttaag ataattttgg ccggtgaatt gtgtaagagc cgtctgcgcc ggcccaccgg
     1861 aaaaagaagc atcaagcgcc gatcccgtca catccataac cgtccgctga tcgggattcc
     1921 aaccgaatgt tcccttcaat tggaagctat ctgcattata accatttgga ccagtaatac
     1981 tcacgtcaca ccctacttgg attattatat ataaaatttt atcttttatt tcttaatttt
     2041 gtcttatcaa aacgctgaaa aaacagcaat ttataggctc accagccttt tctacccgtg
     2101 tcccactcta ataaatatac caagcttatg caaataaata tgttttatgt atatataaca
     2161 aacagttaag ctaaaaatta taccaaaacc gcgcggcggt aactcaatgc ctcagcaaca
     2221 tgaatttttt caatattctc aaatgcttgc ccaaaatcaa ctgcacttaa atccgcaatt
     2281 gtccgtgaaa cgcgtaaaat cctgtaatat ccccgcgcgg aaagcttcga tgtttccatc
     2341 gctttatcca gcaaaacctt cgcctcagga cttaaaaccg caattttctc aagaaccgcc
     2401 ccatccgcat acgcattcag agtcccttca acgcccaacg cctgaaaccg ctgatgctgc
     2461 aattcctgcg ccgctgcaac ccgcgcagcg actgtctccg aacgctcacc tcccgaagca
     2521 ttcaaatccg agagcgtcac cgcagaaaca tccacatgta aatccatccg gtccagcatc
     2581 ggcccggaaa ttttcgcctg ataatcactc gcgcagcgcg gcgctttcgt gcactcaaga
     2641 tcaggatcgc ccaaatgtcc gcaccggcac ggattcatcg ccgcaaccaa ttgaaacttc
     2701 gccggataac gcacatgcgc attggcccgc gccaccagcg cttcattcgt ttccaaaggc
     2761 tgacgcaacg cttctaacgt cccacgtgaa aattccggca actcatcaag aaatagcacc
     2821 ccgttatgcg ccagagaaat ctcccccggc ttcacccgtt gccccccgcc aataagcgcg
     2881 ggcagcgaag ccgaatgatg cggatcacga aacgggcgtt gctgtaacaa tccgccatca
     2941 ggcaaagacc ccgccaacga atgaatcatc gaaacttcca aagcctcgcg cggggacagc
     3001 ggcggtaaaa ttcccggcaa acaagaagcc agcatcgatt taccggagcc cggcggaccg
     3061 atcattagca aattatgccc gccagccgcc gttatctcga gcgcccgttt cgccgtctcc
     3121 tgccccttca catctgccag gtccggccct gcacagctac catcgcttgt aagccgcgct
     3181 tcgggctgcc ccatcacctg cgtcccttta atgtgattaa tcagttgcaa taaatctttt
     3241 ggtgctaaaa tatcaaggtt ccccacccat gcggcctcac cgccgcagct tgagggacaa
     3301 atcaacttgt tatcgtttcc agccgcgtgc attgctgccg gaagtacacc agttaccgaa
     3361 cgaatagagg catctagtga taactcacca agcactaaaa attcagccaa ttcttctttc
     3421 ggaattattt ccatcgcggc caacagtccc agcgcaatgg gaagatcaaa atggctgccc
     3481 tcctttgcca gatccgccgg cgccatattc accgtcaagc gcttgggcgg caaagcaatt
     3541 cctagcgcat gaagcgccgc gcgaacccgc tccttgcttt ccgccacagc cttatccggc
     3601 aagccaacaa tcgtgaacgc aggcagcccg tttgagatct gcacctgcac atccaccggc
     3661 tgtacatcaa tcccctgaaa agcaaccgtg ttaacccgcg caaccatgtc cttttcccct
     3721 tctacacgta tgtccacaga tttcacaaac cgatgaaaat acctgtttct ctgcgcttta
     3781 tagcatatgg ggataaaaaa gaaatggccg ttaagaactt gtttaccttt acccgttaaa
     3841 cacaaggata caagccgcgc agaaatgaaa aaataaagcg cgcaccaaaa gaaggacaaa
     3901 gacaatgttg agtgaaaaag agctggccaa actgacaaat acgctgcaaa tcccgatgat
     3961 tgttcaaggc atcctggacg gacaggaatc attcacgccc gatatgcaat atggcctgca
     4021 cgaaatcctg agcgattacc aaccagattc cgcactgcta agcatcgcgc tgggggcgca
     4081 caagatcgcc gcgcatttcc aaaaccacag ccccaatatg gcgatcctga aactggaatg
     4141 tgaacgcatc atttccgatt acgctgaaat ctgggtgcgc aatgccgaat ccaaaaccct
     4201 cgatgataat ctggtcttcg atacgctgga acaaatcccc gaagaccttg agactctcgc
     4261 cgaactgctg gaaatcaacg ccacgctctt aaacgcgcac aatgaagaac tggctaaaat
     4321 ttgcgaaaca ttcgcgctgc aagcccatgc gcaagtcctg atcgccgata cattcataga
     4381 cttgatggac gaaggcgcag aacttgcaga aacatatgcg cccacgacaa caatttataa
     4441 cgacaatgtc atcccattcc cgggaaccat gaacgcatca taaaagtttc attaacctcc
     4501 aaaaaacggg ctttcagaga tgaaggcccg tttgattttt agcatcaacg acagggaggg
     4561 gtgtctctac tttacgcggt ctgtcctgta ataaaacaac attgcctttg ggttctattt
     4621 gtattttctg attaataaca cccgtccccg ctttgtaaaa aacttcacca acacgataca
     4681 cgccttcagc agttccgaaa cttaatgcta ctgttgcgcc cgtccctgca cccaaagcat
     4741 acccacttgc ccccaaaaaa ccatgcacgg tcgtatcttc aatcggatta atcgccaacc
     4801 acggccccca tgtaacagca tgacccgcaa cggtcgcaaa attgtgcccc ccggtacaat
     4861 aaaaatccac agcgcttacg gccgttacaa caccgcttcc cacaagggta agcggaatat
     4921 ccgttttttt tcgctgcaat ttcggaatat tgtaaccggc ctgatcaagc atcgtatttt
     4981 ctggaaacgc aaaagctttt aactcttcac cgacaccttc cgttgccctt tcaaaatggg
     5041 ccttccgtgt aaaatattta tgaagcttat ggaccgtatt acccccgacc atcccgccaa
     5101 tacccatcca tgctgcttca gcattcaacc ccttcgccag agtaaaaata tagtcactct
     5161 cttgggcaac atgcgctgcg ccagcaataa acccggccca gcccaggaaa ttctttcggc
     5221 tactgcgcac tgcaaaatca ccaacacttt tccaaaaaga tttttgatcg tccttgtgct
     5281 ccgcataaga tttaaacgcg gcaattctgg cttttttgat actgcgcttt tcagcaaaac
     5341 gacaaaagtt gacagttaat cccggtgcat aacaaacagc agtctctgcc ataaattcct
     5401 taatagattg tgctccataa ctgatcctct gcgtcacagg tttcacagcc tgacgcattc
     5461 caaaagacat caattcggtc tcttgcctga atagctcttt aactggacgt gtagccctgc
     5521 cccaggcttt tctgatttta ctttttttat aaacagcgct agctgcaaac attgcaagaa
     5581 ctgccgtcag tccttcccca tatgttactg aaggtatggc agcgtgcagc gcaaaatttt
     5641 caccgatagc gacagccgcc acctggtcct gacttgtttc aatcgcctta acaaaagtgt
     5701 cggctgccgg tgtaccgggt ttgataatca tggagacatc aacacccttg cccatattaa
     5761 cgtaaccctt attcaccgcg tattcgcggt aatcattaag gacaccatgc gtcgcaagcg
     5821 ccgctttatt gatcgtcata caactttcct gcgacaggac tttatccaaa ccgacagaaa
     5881 gtgccgcatc tgcgacaaaa tctggcgcaa tcattttcac aagattatga gggtgacagc
     5941 cacccgtata attcggatca acaaggctat aatctttttt gatttcctta ggctgaccgc
     6001 caccaggcaa aaacattgtt gcatccatcc cggtcgcggc ggcttcttcc acggaaagtt
     6061 ttgtcgctcc aacattcagt ttgccgttaa aagtcagcca cgtcatataa gcagccaccg
     6121 gcgcagataa agtcaacgcc atcttatggt ctttgatttc ctcccacata tgcgcgctaa
     6181 atcttgccgt gctttgcgca ctacgtctta ttggttgtgc catgggttga gcggtttccc
     6241 gctccagcat ttttaaatat cgttgttggt cttcggataa atatttaacc ggcggcacct
     6301 cttcttcggg taagattgaa gataaatacc cggccagttg atccagcccg gcgcgttttt
     6361 ggataagttc ggaatcctgc tgtgctaaag gatttctaag acgtgaacgc gccgctccgc
     6421 gctcaatctc gatcacttca cgggtaaaac gcagcaactg cggatgttga caaaacttat
     6481 cggcccaaac ttcagaatcc ttacctaaac ccagcaattc ccacgctgct tttgatccgg
     6541 gattatccgt tttgcgcgtt atataatgct gtttccacct gcgctgcatt tcaggattat
     6601 caacaatcct ggccaatata tggggtaaag acaccgattc cacagatgca gacattcaat
     6661 agcgccccta acgccgggga atctgaaatc cggcatccat acaacccttg agaacctcgg
     6721 taaaaacaca ctgcgtattg tcccgatagt gggggggaat atttctcagg ttagaattga
     6781 tgttagccgt ataactagga acgcactcgc catgaacgcg ggccaagtga atcgtatatt
     6841 ccttcaaaga agactcaagg cttttacgtt tatccgcatc agacacgttc gtaagatcgg
     6901 gatcatccgt acatttcttt tcattatttg gatcggcctg acgccccgcc tcgcaggaca
     6961 cataaatgct atcgtccaga taccgcgtat attctccgca atcgaccgga agacgctttt
     7021 taccattaat tgtatacgcg ccatgcggat gttcaataat cggatcggcc tgcgcctgaa
     7081 aggttggcaa tacggaaagg ctggcaagca acgcaccgcc aaaacaatct cttaaagaca
     7141 taggttaatt cctcaaatgt taaaaaatta ctcataaatt ttctgaaaag ccttctgata
     7201 caaattaaat aagaatgact ctcatttgct attgactcaa catgagcaat tgataatcat
     7261 tctcattatt aaatcaagtg cttttttgat tgggaatcta aaaaatggaa aaagacaaaa
     7321 aagaaaaagg tatgctgtca aaagtttttg ggggcgctgc caagcttgcc acaatcggcg
     7381 ttgccgctgc tgtgacatgg caaattcttc tggatcctat ctttttcccg atcattcata
     7441 acctggctgc ccacccgacg gcggcagcct gggtttcttt tattcaggat cacacggcct
     7501 ttattacaga ttttatcgga ttcaccggcg acgggggctt attacaatcg gaatggggag
     7561 acgctcttct ggccgactat tacccggacc cggtaagtag cggaacgaca gccgtacaac
     7621 aaggtactag tgtagcaaca gccgttccaa caccggcacc taaaatggat gctacgatgt
     7681 ttttaccgca gccgggctaa actacgatta acctgcctta ctcaccatag gaccaaccgg
     7741 gccgccgccc agaatatgca ggtggaaatg tggcacttcc tgcccgccat cgacccccgt
     7801 attggcaatc gagcgaaaac cgtttttgtt caagccctgc tcatttacga ttttggctac
     7861 ggctgcaaaa aatccggcga cttcctccac gcttgcccgc tttccaaaat catcaattga
     7921 aacataccga ccggtcggta tgataagaat atgcacaggc gcttttggat caatgtcctt
     7981 aaaagccaac gcatactcat cctcatacac cttgtcgcaa ggaatctccc cgcgcagaat
     8041 tttagcaaaa atattttgat tgtcataatt cattataaaa atccttccct tcaataaaaa
     8101 ctcgcactat ataaaacaca tgttgctgag aatgctcaaa tcaaaactcc accgcgccac
     8161 cgtcacacaa agcgatttgc attacgaagg ctcgataacg attgatccgg agttgatgaa
     8221 tgtcggtgaa attaaccctt acgagcaagt tgatgtcctg aacatcaata acggcgagcg
     8281 ctttaccacc tatgccattg aaggacagcg cggttcaggc gtaattggta tcaacggtgc
     8341 cgccgcccgc cttgtccagg ccggtgatct ggtaatcatc tgttcttatt gcgaaatcga
     8401 aaaagaaaaa gcgcgcgggc acaacccgac gattatcctg ctagatgaca ataacatgcc
     8461 gaaaaccaca taatcaaaca atacatcgat tgtctgtaag ccaggcaccc tcaccccacg
     8521 tggtatcctg atatttggag ggtgggcctt gctgcgtcgg cttattatcg ctcggaacaa
     8581 ctggcgcacc cgaacgacct gcttccgggc ccgtcgcaca cgcagcactc aaaagcatcc
     8641 cggtaaacat aacactcaaa gtttttattt tcataatcta attaatagtg ggctcaaatg
     8701 tccatgtcaa gtattataaa ttcctaatgc ccgcggctcg ccttttcctc gtgcccggaa
     8761 atcccgaaac gccgctccag caccgcaaaa acatcttccg gcgcgatatt atgatacgac
     8821 aaaagcacca gcaaatgaaa caccagatcc gccgattcat tggttaaagc ctgacggatt
     8881 gcctcatcgc ctgatgattt ctccaaccgc agcccttcaa caattgtctc ctcggcctct
     8941 tcacgaattt tctcggaaat tttcacactg cccttatcat acagtgaagc cacataagaa
     9001 ctttcaggat cggctttttt ccgttcctgc aaaaccgcat acagcttttc taaaatatgc
     9061 gcactcataa tctaaaaccc ataacctata atcgaacgcc tatacccgcc tcagccatag
     9121 cctgcttggc ctgcgcaatc gtatattccc caaaatgaaa aatcgaagcc gccaagaccg
     9181 cacttgcatg cccctcttta acaccatcga ccagatgctg caaattccca accccgcccg
     9241 aagcaataac cggtatcgga accgcatcgg aaaccgcacg cgtcaactca agattaaatc
     9301 cgatcttcgt cccgtcacga tccatcgagg tcagcagaat ttcccccgcg ccgtactcgg
     9361 ccatcttgac cgcccattca accgcatcga tgcccgtgcc cttgcgccca ccatgcgtaa
     9421 agatttccca gccttcgcta ccatcttctt tctctttggc atcaattgcc acgacaatcg
     9481 cctgcgaccc gcatttttcc gccgctgcac gtacaaaatc ggggtttttc acagccgccg
     9541 aattaatcga aaccttatcc gcgccggcac tcaatagatt acgaatatgc aaaatcttgc
     9601 taatcccgcc acccaccgtc atcggcatga aaacctcatt ggcaacatgc tccaccatat
     9661 gaatgattgt atcacggccc tcatgcgtcg ccgtaatatc aagaaagcac aactcatcgg
     9721 cgccctgctc attataaatc ttggcttgct cgaccggatc accggcatcg acaatatcga
     9781 caaaattcac gcccttgacg acgcggcctt gatcaatatc cagacaggga ataatgcggg
     9841 tttttaacat agagaaaggc ttaaaggttt aactccgtgc gcgcaatata caaatctagc
     9901 tcattggaac ttttggagca gtatcttcaa caacgatata agttaagcca ttttctttta
     9961 ccgtaggcgc cactgctatg cctgcgacag cttgagagaa tccttcaaaa gcagccagcc
    10021 ccgaatttct tgtaagcgcc aaagctatat ttggattctt aagatgattt tgttccaacc
    10081 catcctttaa gttcattgct tcttgtgctt tatgaaacgc cgccataaat gcaagcttct
    10141 cttcacaaga agctcctcct aacacctcag caagaaaagc atcaaacaca cttgtatatt
    10201 tcccagacat actcaattct ccgcaacaat tacacgcggc gttataacga aaaacataaa
    10261 tatgttaatt aaaattaaga agacaaaacc gccagcgctt cttccggatc gacgcgctta
    10321 tcatacaaag ccttcccgac aactacaccg ttgaggccat gcgtcttact ggcctcattc
    10381 accgcataaa tatcggcaat cgagcccact ccacccgaag caatcaccgg aatagacgtg
    10441 ctctccgcca gcgcgcaggt tgcttcaaca ttcacgcctt tacccgtccc gtcacgatca
    10501 atatcggtat atataatcgc cgccacgccc gcatcttcaa aatgcgcggc caattcgctg
    10561 gcctgaatgt tggaaccatc gatccagcca tcaaccatca cctcaccgtc aagagcatca
    10621 atccccacgg cgatctggcc cggaaatttc cggcacgccc attttacgag ttcaggatcg
    10681 cgcacagcgg ccgtgcccaa aatcacccgg cttacgcctg cttcaatcca gtgctcaatc
    10741 tgcccgcgat tgcgaatacc gccaccaagc tgcaccggaa tatcgaccat ctccaaaatc
    10801 gcccgcaccg cacaatgatt tttcggcacg ccttcaatag cgccatccag atcaacaata
    10861 tgtaaccagc taaaccccgt ctgcgcccac tcgagcgcct gccccaccgg atcttcgtta
    10921 taaaccgtgt cacgcgtcat gtcaccttgc acaagacgca cacacttgcc acctttcaaa
    10981 tcaatcgcag gataaattat catgaggaaa agatataaag aaaacgccag cctcgtacaa
    11041 gagaccttca caataacaaa agacaccaaa aagcttgact ttacataaaa atacagctat
    11101 catcccacac agttttacag aatttaaaga aatagggact aaaaatgtca aattctaggc
    11161 tttcaactct tgtatttgat gacgcgaaga aaaaagttca caacgcttcc ttagaaacgc
    11221 tacagccggt cattggccgt ctggaagaaa cttataagga aaaaccaaaa gcaaccgcca
    11281 ctttaatcaa tattttattg gaacacccca gctataaagt cgtctgcgcc cttgcaaaga
    11341 gcaagaccct ctctcatttc cctgaagcgc aaatgaatct ggcaacccac gaaagactaa
    11401 agcacaaatc ctttctcgcc gccttgccgg gcctaacaga tgacgtgcaa gtacacttgt
    11461 cacgtttagg ctttccagaa gcggccccgt caacaaattc taaatcaaac actttagccc
    11521 tcgaaaacaa caatgaaatc gttctggaaa caggaaaaaa ctcctcaaaa acagcaaact
    11581 tcaatactgc aagccacgac cataacaacg gatacaaatt tgaaatgtta cccctcatta
    11641 cacaggagga caaaaaccgg cgcacaaacg cacaatatat aaaagcaacc aaaatcgcat
    11701 tggcacaaaa tccatatgct aacagcaaag aggcacaact caaccttgca acaaacccgg
    11761 atgaagacat tcaactggta ctggctggac gtaaaagtcc gatcgatgaa gacgcccaga
    11821 tcgagctatc caaaagcccg ttcccgagcg tacaggcttt gatcgcaggc aacataaatc
    11881 tgacaagcca acaagcacaa ctcaacctaa gtaaaagcga tcgtcaagac atcacagccc
    11941 cattggctgg aaacatctct ctgaccaacg aagatgcaca gcataattta tctaaaagcc
    12001 cttgggccaa aaccagaatt gcgcttgtga ataatgactc tttaacagac cccgatgcgc
    12061 aaaacaacct cgctatagac tccgatgaat ctgtaagatc tgctgttata aacagaacaa
    12121 aaccatataa aagcggacac tacctgggtc atctgcatgg tttcgccaaa aattaccaag
    12181 aatataaact aaaagacacc ctcagcgatc tgaacatcta aagacaacgc ttcaaacact
    12241 gaaacaagac atcggcagcg ctcagtcaaa ggatttgact gggcagccgc accaaagcaa
    12301 acataattct agaccgtccg accggaccgg tgtactaaat aatattctat attgtccggt
    12361 ttaggatatt ttttaaaaga cgtatgaagc tgcaacgata caacatagca cttttacctt
    12421 cagaccatac tttgcaaaca accatggtcc atatctcaag actctatttt cttgaagatc
    12481 aggatgaata tattcttgga cccgaaggac tgccccatat taccttatgc caatttaaag
    12541 ctgccactct cgaaaatgct gtcaccgctt ataatgactt tttatcaaat gaatccataa
    12601 gcactattaa agtaattatc gagaagttta atataagacc cggtaagctc gttaatactg
    12661 gaaaatttat tgctgagtac aaaattcaac ctcatccaga tttgatcgct ttgcaacttc
    12721 ggtgtgctaa aaaacttgcc gctcaccatc tcgaaaacct tacgccctca gaagcttata
    12781 gtcctcatat caccctggcc agacttcctg tcatgacaaa caaactccct acaaagcagg
    12841 acttaaattg tccgctctat atcgatgctc acttatcgtt gggcttgtct acagaagctg
    12901 gtgttttcgt gaaagagttg tcagctcaca ccctctaaaa ccagttcgaa agtagattcg
    12961 cgcggcgctg taccgttctc tcttcgcaaa tcctccagcg taaccttgtc tcccgaaggg
    13021 tcatccccga ctaggcggcc attgcgctca gccaactcca tctgttcttt tatatactct
    13081 gcgacagggg ggtcgagcaa ctcatagcaa atatcatctt gctcgcgaac gccttcacga
    13141 aacatcttgg catcaatcgg ctgatattcc gaaacagcat caatcatctc ttgtgtgatg
    13201 cgttgaatgg gaaacaaccc acgcggctgc ccaaccagct ccacatagtg aactcctttt
    13261 atgttctttc catcgaccag atagtcctta cggtcctcat ctttttcaaa ataagcaaat
    13321 acaagcttat ccggcagcgc tatggaattg aaaatacctg ttaaccggcg cactgcatcc
    13381 ccgacatcac cgccagcacc cagataaact tgcgcatgcc tgatattttc agcaggaagc
    13441 cacggcatcg aaatgcgggc catttcatct cgttgatgcg ggttaaaagg gttacgatcc
    13501 ccttcaacac tatccgtttt atcattcaac accagattga cgtccaaacc ggaatgacgc
    13561 aacgtttcca tcaaggccct gtgaccgcga tgaggaggct gaaggcggcc aaatagaaca
    13621 attttcttaa acccctgttc tttccatgac attaattgtt caagggtagg cgcgatatta
    13681 gccaaggaaa aatccccgcg caaagcattt gatttcatga ctgttctaaa ccgttatatt
    13741 caatagcatt tcgttctgat cgcgaactta tgcaaagggc aaattcaaat caaccccttt
    13801 tttgaacttt ataaaaactt ccggaactaa ctctgctgct caaccacgct tggatcaatc
    13861 accttataat aataataacc cttgagaatt tgatcgggca accgcacgga ataaggatgc
    13921 atgccaaagc gcacataacc cagcgcctca taaatttcga ttgcccggct catcgtttcg
    13981 cgaacctcga gattaatcac cgcgaaacct tccgcgcgcg caacctcttc agccttttca
    14041 accagcatct tggccaaacc atgcccgcgc gcccacggcg ccacaaaatt tgtcgtcaac
    14101 gtcacaatat gggcctgagc ctcattatga gcaggcggct tccacaactg gcaggttcca
    14161 caaatcaccc cgtcgagccg cgccgccagc aagatccgcg ctggcatggc gagcacgcct
    14221 tgccaatacc gctccatcat atcgcgggcc ggcaaatgca cccagccaaa tccaccgccg
    14281 ccttcaatcg ccgcatcagt ggcatcgcat agatcgttga gatccgctgt acccagcgac
    14341 tcgataactt ccacagtcgg cacaggggcc aaaggcttca attttgtttg atcgctcatg
    14401 tagacctcca ctcctaaaaa cgttttacgg gttccagccg ataaaatcgc taatcatgcg
    14461 caacccagcg ctctggcttt tttccggatg gaattgtacc ccaactacat tatcccggcc
    14521 cacgacagcg ggaaacaatc cgccataatc aaccagtcct aaaacgtgat ggttatactc
    14581 acattcaaac ataaaagaat gaacaaaata gtaatgatcg ccaccttggc tagaactttc
    14641 tgtgctgcgc agcacaaagt gccggttatc ttgttcgccc gaatgtggta gcatcagttg
    14701 attccatccc atatgcggaa tttttaaggc cggatcgtta atccgttcag ccagaggaac
    14761 aacctgtccc ggcacccagt tcaaccccgc atgcacaccg tgctccagcc cccgagtcgc
    14821 gagcagctgc atcccgacgc aaatccccag aaacggctta ccatcttttt gcacgcgttg
    14881 ttcaagcgcc tcggctatcc cctcggtgct attaagatta ttcatacaat cgccaaacgc
    14941 cccttgaccc ggcagcacaa cgcgatcggc ccgcaacaca tcttcggcgc gccccgacac
    15001 ttgtacttca gcatcaatcg cctcattagc aatcacgtgc tcaaacgcct tggccgcgct
    15061 gcgcaaatta cccgatccat aatcgataat gacaatggtt tccttggaca tgagaaggtt
    15121 ttcgatatat atgtctgctt tgtcaatggc taagcaaaaa atagtaaagt aatgagcatt
    15181 aacttttatc gctggaacga aatcgatgag accacaaaag caggtatcat gcgccgctct
    15241 caagccgaca tcgatgacat gatcgccatc gtcgaaccaa tcattaaaga cgtaaaagcg
    15301 cgcggtgaca cggccctgat tgactatgcc aaaaaattcg ataaagctga aatcaccaat
    15361 ctcaaatcca ccgccgaaga attcgctgcc gccgaaaatg ccttggatga agaccttaaa
    15421 gccgcaatcg cccacggcat acgcaacgtc caaaaattcc acgaagaaca gatgcgccgc
    15481 gctgaaaaag accgcagctg gatggtcgaa attgagcccg gcgtttttgc cggtgagcaa
    15541 atcaacgcta ttgaatccgt cggcttgtat gtcccgcgcg gcaagggtgc cttcccttcc
    15601 gcgctctata tgctagccat tcccgccgtc attgcgggcg ttaaaaatgt tgccatcgtt
    15661 tccccaccta cccccgaagg caccaccgat gctgccacac tctacacagc taaattatgt
    15721 ggcatcaaaa atgtctataa atgcggcggc gcccaggcca ttgccgcact ggcctatggc
    15781 acgcaaacaa tcccgcaggt caaaaaagtc ctcggccccg gttcacctta tgtcgccgcc
    15841 gccaagcgcg tctgctccgt tatcatggac ccgggtatgc cggcagggcc ttctgaatcc
    15901 atcgtactgg ctgacgagac cgccgatccc gaaaacacct gctgggatat tctcaatgaa
    15961 ggcgaacatg gcgaagacag cgccgcactt ctagtgacgc atgatgaaaa acttgcaaaa
    16021 tttgtgcagc aaaatttacc gtccagaatc gctgaattac ccgagccaca caaaaccatt
    16081 tgtacaaaag ttatgggcca agacggaaat gagcgcggct atggcggcat tatcctcacc
    16141 gaaaatctga gccaatccat tgagatcgcc aatacctacg cccccgaaca cttgctgctt
    16201 aaaatcgctg atcctgacag cgccctgcac agactaacgc acgctggcga aatcctcatc
    16261 ggtgaataca ctgccttttc acttggcaat tacgccaccg gcgtcaatca cgtcctgccc
    16321 accggcggct gggcccatac ctattcctgc acctccgttt gggactttct caaacgcacg
    16381 tcattgtcga aatgcacaca cgaaggtttt gaagccatca aaaactctgt catcaccctg
    16441 accgattacg aaggcttccc cgcccacggc gacgccttaa gaaaacgcaa aatctaaaat
    16501 gagcgaagat attcattaca tgactcctgc gggctataaa gctctggccg atgagcagga
    16561 tcatctcatc catacagagc gcccgaaaat ctgcgaaatc gtttcctggg ccgcaggcaa
    16621 tggcgaccgc agcgaaaacg gcgattacat ctataacaaa aaacgcctgc gcgaaatcga
    16681 ccgccgcctg cgttatctca aaaagaaact cgacaattgc aaaatcgtcg atccgaaaga
    16741 ccagcaaaat ttaacccgcg tctatttcgg cgccaccgtc acctacgtcc gcgaggatga
    16801 cagcaagatt accgtccatc tggttggcgt tgatgaagcc gatttttcac aaaataaaat
    16861 caactggctt tcccccgtcg gcaaagccct gctcaaacac gaagtcggcg acagcgtcaa
    16921 agtccgtgtc gacggccaaa tcgaagaaat cgaaatcctc gaaatcaaat atatgatcga
    16981 ctaaatatcc gaagaaaact ctttacgaag ccagattgca aaaagaggat catcaaaaat
    17041 aacccgccct ttgccgctac cttcatccaa aatatattta tttttaagcg cattaatggc
    17101 cgaagccaaa gcggccccac tagtaacttc gtatttatca agattttctt taagaaaaat
    17161 ttgctctctt tcaatagcag ctaaacgcaa aatgcgctgc tgcatcaaag tcaaagaccc
    17221 caaaagcgtt tgataagcat aagagttctg accgacaatc gtattcaaaa cctttttaac
    17281 ctcgcccatg ccaatatacc gctcaccctg agccaccaaa tgaaaacacg ccatctggat
    17341 atagttaggc gtattttgca cctcatcacg caaaaagata acagcgtccc gatcacactc
    17401 aacatccaca ctggcaaagc gctctataat ccagtcaata aactcatcgc cgaaaaccgg
    17461 aaaatcgagt tgctgacatg aacgataaaa aggccgcgcc tgatcgttca acatgtcatg
    17521 gataagactc tgacgcgaac ccgtaaacaa aaatacagta ttgcccaact gctgcatttt
    17581 cgtacgcaat gtcgcctcca accatccatt atcatccaat tcagccattt gctgaaattc
    17641 atcaatgaca aaaacaaccc gctcgcccag agacgaaatt cccgaaagca catcttgaat
    17701 ggtttcttta acatcccgat catcagaaat atccgccgat aaagaaaaag aatattgccc
    17761 gctttcagag acgtcccacc caatccgtgg ccgaaggtgg ggaatagttt ccgcccactc
    17821 tttaaagcgc tgcccccacg tactctgcgc ccgcagcgct cttaaagttt gctgtaaaaa
    17881 atcacgatgc gacgtgatat taaaaatatc cataaaaaga caaacatgcg tatcctcaaa
    17941 accccttaac agattttgca caaaagacgt tttaccaaaa cgccgcggac ccatcagcac
    18001 gacatgaatg cgattatcaa gataggtccg gacaagacta ttcaattccg gacgcggcaa
    18061 ataatattca tcggaaacgg gatttccaaa actaaaagga ttcatagcgc tgcaaccaat
    18121 tctaaaactg ttagctttta agcattttaa ttactaacag aactattagt aattcgcaag
    18181 caaaaaagct aaaatccacc cctacaaagc gcctttagtt gacggcaatg cgtccgccgc
    18241 gcgtggatcc gtctccagcg ccatgcgcaa cgctttggca aaggccttaa agctcgcttc
    18301 gataatatgg tgattattgg tcccggccaa ttggctcaca tgcacggtaa tcccagccgc
    18361 acccaccagc gcccggaaaa actcttcaaa cagctccgta tccatttccc ccagccgctc
    18421 cacggtgaac tctacatccc acaccagata aggccggttc gataaatcca gcgcgacgga
    18481 tgaacgcgcc tcgtccatca ccagatcgaa atgcccgtaa cgacgaatgc cttttttatc
    18541 gcccaccgct tctttaatcg cttgacccag cgtaatcccg acatcttcgg cggtgtgatg
    18601 cccgtcaata tgcagatcgc cctcacacgc aatcgtaagg tcaaccagcg aatgcttcga
    18661 aagctgctcc agcatatgat cgaaaaatcc aatacctgtg gagacgtcat aaacaccccg
    18721 accatcgaga ttgaccgtca cggaaaccga tgtttccttc gttttgcggg tgattgtccc
    18781 caatctttct tttgcgccgt ctttaggcat ctttgcatgc tcccgtattt aggctacgct
    18841 tttaaaaacc gcgccaacca tagcacaaag ccgcatgaaa tttccataca aaagactgtt
    18901 acaaaccatt acagccgtag ccctgctgac aggttgcggt ggctccatcg tcaaggaagt
    18961 tgattacgcc cccacacccg acatcccgga aaccgcacat cccacgccga taaaatacag
    19021 cggcatcgaa cttctcctcc cgcccggcat gaatatcggc gaggcgcgcc acggtatttt
    19081 ctgcaattcc ccggcctatc cggtgaaccg caacgttatc gccaaggaaa tcgatcacaa
    19141 attcctccgt cagggcttcc atgatgcgat ggaggccaac ggctacgacg tcgtcggctc
    19201 gctcgacatc gccttcgacc cggatgatga agaacaacgc gccgaatatt cgatcaaagg
    19261 taagctcaaa gacgcccagc tcgatatgtg cacatcacaa gaaggcgcgc tcaactgggc
    19321 cacacccgtt cccggcgaac atggccgcat gtatatcgcg atcgactgga gcgtttacga
    19381 cgttttacac agaacaattg tctacaaaac ccgcaccgaa ggctatagca accggaacat
    19441 ccctaatacc gaaggcatga ccctgctctt ccaggacgcc tttgaaatgg cggtgcataa
    19501 tctggccgca aacgaaccct tccgcgcttt agtcgttgat ggtgtaaagc caccgcaaaa
    19561 atggcccggc aaagacatgc gcggcgaaaa aaccgattct cgcccgcgcc tgtttgatga
    19621 agatgaagac gtcaccctgt ctgaccagcc cctctcgcgc caacccttcg ccaaaacagc
    19681 cgaagacggc cgcaaaactg ccgtcctcat tgaaaaagac ggccacggct ccggcttctt
    19741 tatcaccaaa cagggccaca tcctcaccaa cgcccatgtc gtcggtgaat cccggcgcac
    19801 ccgcatcgtc accgccgcac gaaaaactgg catcaccgcc gagatcctgc gtgttgaccg
    19861 cgtccgcgac gtcgcgctct taaaacttga agaaatcccc gaatggctgg acatccaaac
    19921 cctgcccgtc cgtctcgact ggccaaaggt cggcgaagat atctacgcca tcggtagccc
    19981 caaagactgg aaagccttca aaggcacaat cagcaagggc atcgtatccg cccatcgccg
    20041 gaatatgaaa tatggcggcc cgcgctccaa ctatatccag ggcgacatcg aaacccaccc
    20101 cggctcttcc ggcggcccgt tgctcgatga atacggcaat atcgtgggct taagcgtcaa
    20161 aggcgaaccc ttcgccaacg aaaacggcgc ccgcatcggc atcggcctca acctcttcat
    20221 accgattcgc gaagccctcg accggctcgg catcgcctat gaagggcaat aacccccggt
    20281 gttcgtcatt gcgagcggtc gaagagcgcg cggcaatcca aaaatcctcc ctttttgtct
    20341 ttcccaccca acatgcaaat accaaaaaac gcttttttct ttaaaaaaac cgcccaccca
    20401 aaagcccttt tttataacgt aaaagctatt gtttatcaca aaacgatacg ttttcaaaag
    20461 cgcccaaagc cgctccagtc gcctgctata accccataaa caggcttaaa ggatattttt
    20521 gaagcacgaa gcgctataaa tattgacatt atgcaatatt tcaccaccct cgcgtcgatt
    20581 ttaatcgatc ttgcgcaaac ctccataaat cagactcttt atttcagagt tatgaataac
    20641 atatgtcggt ttttcttgca ttttcttccg tcttttgata cgtatcgtag tgtaatttca
    20701 aacaggatga aaaagggagg ctcgcaatgg ctttacctaa attcaacaaa gaaacgttcc
    20761 gcaaatcagt tctaggtgct ggcggtacga tcgcattagc actgagcgtt tcaccagcaa
    20821 atgctgaaaa cgacatatgt ttacctttga cgcagatcaa tggaattaca ttgagtgcgg
    20881 acgcaacagc agacagacac tcacgctcta accctggcgt tatctcaatt gcaaccttac
    20941 ctggcggaga catgcgtaat agccctgaaa atttaacaaa agttttccga aagaaaggaa
    21001 tcggtgcaga ttgtttttta aataattcta tggcgtctaa ctatggaact gaattttctt
    21061 tgtatgtggc agggaaccct gtgaagttta atggtaaaac gtctttcggt attcaggaat
    21121 taagagacaa tccagaaatt ttaagaacag cacatgcaga agctattgtg tctaaacaaa
    21181 tgctagcagg aacagttaat acgccgcgcc tagcaggcca gtaacgaata attaataacc
    21241 aaaaccatca tctggagagc tattggcttc tgcgaattgt agcttctgtt ctacagtagg
    21301 cctgtcgatt tcaatgccct gctgcgcttt aatctcttcg gcgcgattaa tgaccctttt
    21361 atctaaactc tccaagctcc gaccagtttc aacagcattc tcagcaaccg cctgaatttg
    21421 ctgttgaact ttattatgct gttgcaacca ctcctgtgcc cacgggtgcc cctgcaaatt
    21481 agacgattga agctcaccac tatcgagttt ttcctgaatc agtttcgcaa ttgcctgcct
    21541 gcgctcatcc tgatcatgaa tccccataat gcgatcatat tcatcctgat caaccagccc
    21601 ttgctcctgc aaatcagaaa gcaaatcttc cgcgaagctt tcgccatatt gttcagccaa
    21661 cgcatcttcc attgcatgaa gctgatccaa caacatcagc aacataatat ccccgtggcg
    21721 ctgcgcagat ttttcgtcct gcgcagcgcg gagcgcttca atgcctgcgc gcgcttcatt
    21781 gatctgagca ttaacctgcc cgcgctccaa ctgcccctgc ccgacagcaa tttcatcttg
    21841 gtgaagttta aagctctctt cactgcccga cgcataaaaa ttttgcctgg aggctttctc
    21901 aaattcaccg cccaggccaa gcgcttcctc gatcggatcc gcctgcgtag caaaattcgc
    21961 gcgtaaggat tcctccgcgc gcgtagtcaa atcgcttcca ttttcccgaa actgtgacct
    22021 tgtaatggcc atcttaatcg tccccagttg ttacacacgt tcctgttcac actcgccgcc
    22081 ctgcctttcg cctgttttta aggtctttcc agagcttttt gccgtttgtc cgcccctgtt
    22141 tttattgttt tatcaataag ggttgacgcg caccgccttt atggcttttt ttcttgcgca
    22201 tccctaagct aaagtataag tcttaattaa aagttatgga aaatataaaa ttttaataaa
    22261 attgtatcga cttcacaccc gtttttgcgc tttatagata ttacaattga attttagttt
    22321 gattttgaaa gataatacac tcataagatg taaaaaactt ttttgcattt ttttaaaaaa
    22381 aacatcgatt ttctccatat ggttgacata ctccgcgctt ttgcccatat catgggccta
    22441 tgaataatga acatcaaaac tacgcaaacg cacaaagctg gcgcggcacc accatcctct
    22501 cggtccgtaa agacggaaaa gtcgtcatcg ccggtgacgg ccaggtttcc atgggctcta
    22561 cagtcttaaa aaacaatgct cgcaaggtac gccgtttagg gcgtgatgat aacattatcg
    22621 ccggatttgc tggctccacc gccgacgcct tcaccttgtt tgagcgatta gaagccaagc
    22681 tcgaagctca tcccggccag ctcacccgcg cttgtgtaga actggccaaa gactggcgca
    22741 ccgataaata tttgcggcga ctcgaagcgc tcatggccgt ggctgacaaa gatacctcgc
    22801 tcatcctgac gggaaatggc gatgtcgtag agccgcaaga cgggattatc ggcatcggct
    22861 caggcggcaa ctacgccctc gccgccgccc gcgcgctcta tgaccaaaag ttaaacgccg
    22921 agcaaatcgt gcgcaaatcc atgcaaatcg ccgccgatat ctgcgtttat acgaatgaga
    22981 atatcgtggt ggaagaactt taaaaccatg gctttaaaat ctgaaaaaac agacgcgata
    23041 gaaatcccgg ccttttcccc ccgcgaaatt gtcagcgagc tggaccgctt tatcatcggc
    23101 cagagtgatg ccaaaaaggc cgtggcgatc gccctgcgca atcgctggcg cagaagacag
    23161 cttgagccag ccctgcagga agaagtctac ccaaaaaaca tcctgatgat cggcccgacc
    23221 ggtgtcggca aaaccgaaat tgcacggcgc ctcgccaagc tcgcccaagc cccgttcgtc
    23281 aaggtcgaag ccaccaaatt caccgaggtc ggctatgtcg gcaaggacgt ggagcaaatc
    23341 attcgcgatc tggttgaaaa cgccatccac atgacccgcg actcgttgcg caaaacggtt
    23401 caagcccagg ccgaaattta cgccgaagag cgcgtcctcg acgcccttgt cggcccaact
    23461 gccggggaag gcactcgaaa ttccttccgc gacaaactcc gcaatggtga actcgatgaa
    23521 cgcgaaatcg aaatcgaagt gatcgacaat gctagcccca tgggcggcat gatggatatc
    23581 ccaggcatgc ccggcgcctc gatgagcatg atcaatatgt ccgacctgtt tggcaaagcc
    23641 ttcgagcgca cgaagaaaaa gaaaatgcgt gtcgatgaaa gccacgaaat tttgatgcgc
    23701 gaagaatccg acaaactcct tgatgaagac aagatcaccg cgcaagccat cgaactcgct
    23761 gaacaaaacg gcattgtctt cctcgatgaa atcgacaaaa tcgctgcccg tcaggatgtc
    23821 cacggcggtg acgtcagccg tgaaggcgtc cagcgcgatc tgctcccctt aatagaaggc
    23881 accaccgtca ccaccaaata cggccccgtc aaaactgacc acatcctgtt catctcctcc
    23941 ggcgcattcc actacgccaa gccttcagac ctcctggccg aacttcaagg ccgcttgccg
    24001 atccgcgttg aactacgcgc cctcacgcaa gacgatttca aacgcatcct caaagacacc
    24061 gaggccagct tgataacaca atatgtcgct ctgatcggca cggaaaaagt tacgctggaa
    24121 ttcgaagacg aagccatcaa cgaaatttct cgcctgtcat ttgaggtcaa tgaaaacgtc
    24181 gaaaatatcg gcgcacgccg cctgcatacc gtcatggaaa agctgctcga agacatcagc
    24241 tttacagcca gcgatcgcgg cggcgaaacc atcaccatca ccgccgacta tgtccgcaaa
    24301 accgtcgaag aacttgtcaa atctgcagat ctcagcaagt ttattcttta aaaagccacc
    24361 ctttgtggtt caaagcgacc cgttgaatct accgacgggg caacctgttc ttctcccaaa
    24421 atctctgtaa tcggcaacca ttcaacgctg ccttgctcct cctgaatgta gggaataaaa
    24481 ggaacagagc gccgcatatc atcttttctc aaacgcggca tgccaatccg ataatcacca
    24541 tgctgataca aaactatttt gggatcattt tcagccaaac caacctctac ctgctccaaa
    24601 gattggctcc gcattttaaa atcaccaaac gggatataat gatcggcaaa agacacaaat
    24661 aaccgcgaac caactcctaa cgccccgcgc gccgcattct cgaaagcaag caagctcaca
    24721 ggctccaaga agcaataaac agaccggcca taagccttat ctttagtcgc ctgaaaacga
    24781 tagcgcacat taatccattt ttgtgtttgc ggaatttccg aatgtttaaa acccttcttc
    24841 gccattttac caaaaaacat ggaaagatcg cccggcaaag tcagcatcac cggcgcttta
    24901 cgcgccagag cagcctcaaa gaaaagcgca gttttaccca taacagctca catacatcaa
    24961 ataaaaatcc agaactttct accacaaata aaagactatg taaaatattt taagctggct
    25021 ttttatgaac gcgcacgctt aaggtaaagc catgcaaaaa ctcctcataa tatgcgccat
    25081 tctggcttcg accttaagct ccgcaaaagc gcaaaataca cccaaattta gcttcccgct
    25141 cgactgcaca ctgggcacag attgctggat cgctaactat gttgacgttg acccgaccga
    25201 aggtgtcaaa gattttaaat gcaccgctaa aacttacgat ggtcataaag gcgtcgattt
    25261 cgcactcaaa tcccgcgtta taatgaacaa gggcgtcaat gttctcgccg ccgccgatgg
    25321 caccatccta cgcatgcgtg atagtgaaaa tgataacccc aaaacaaagc aggaatacga
    25381 tgccatcacc gccgccaata aagattgcgg caatggcgtc attctcgacc atggccaagg
    25441 tctgcaaacc ttctattgtc acctcaaaaa cggctccata aaagccgcgc ccggacagaa
    25501 aataaacgcc ggtgaaaccc tcggtcaggt cggccaatcc ggatttgcag aattcccgca
    25561 tctacacttc accgtaatct gggaaggcag ccatatcgac cccttcaccg gcatgaccaa
    25621 agaagatggc tgcggcaaat ttaaacaaag cctgtggaac aaggaaatcc catacgcacc
    25681 atacgcctta ttcaacggtg gttttgcaga aaatgtaccc gatttcaaaa ccctcaatga
    25741 aaccggaacc cggccacaag aaccaaccct gaccagcaaa gcctttgtct tttggatcag
    25801 tctgtatcaa atgcaaaaag gtgatcaaat cacgctcaaa gtcttggacc ccgatggaaa
    25861 aaccttcatc caccgtgaaa taacgcaaga cacaaaccgt gcccgccaac attactacac
    25921 aggcagaaag ctcaaggacc acagcctgaa accgggcaca tacaccgcta ccgccacgct
    25981 caaaaccgaa ggttacgaaa cctacacgca agattttaaa acaacaatta aataaaagag
    26041 aaaatgaaaa caactaccga acaagcttca atgcaatctt tatcgcgtga ctattcgcaa
    26101 gactttcgtc ccagtgatgt ccgggacaac gaatttcaag agctaaccgt tgcacgtcac
    26161 gcccacggtc gtcttctgct ggcaaatcct tgctctcaat taaggcaaga tactcaccac
    26221 attcgcagta tggtcttatc gcaaaaacgc aagggaaaag aagccaatgc gcaccaagca
    26281 gtacatgaag aaaggcccga tggctgtatc cgcctagacg atcaggaaat tgaccgctat
    26341 gacaaaactt ctttgtgctt tatatatgtt ccggagactg aattcaaaaa accgccatcg
    26401 gagattaaag ggttgggaac tcttcgcata attccttcat attccggcca gctgcttcat
    26461 gcgcataatg ttacgaaaaa aaacagaccg gaggatctcc ccaccgcgcc gacctatact
    26521 aaccagcgtg atcctgatcc tctcgcctca tggataaaaa atgcaggtga cagatctcta
    26581 tcctctcgaa ctaacccctt cattgtcgaa ttctcacggc ttcacatttc gaaacagcgg
    26641 cgcgtggaaa caggctatcc ctccggcatt tccccaaccg aatttatact cgccgcagcc
    26701 atccgtttaa ccctcgatat gcaccgcgtt gccttgccaa cctatccggg cgcgtgtttg
    26761 gagcactctg ttgtggagcc tgccctcatc aaaatgcgga aacgtatgaa ccagcatccc
    26821 gaaagccagg gagagacatt tgaatattta ggccggaaga gattcgccgt ctacaatgtg
    26881 tggggttgtt atcaagatat acgtaaaaac cgtcccgaaa ttttcgaaca aatgtcctgt
    26941 ttcaactcag agccattgtt tgagtatcta aatttcggca attctctatg cgccgcatgg
    27001 accaatgaac gtttaaaaca aacggaacat atgctgaccg atgagacgtc cgtgcataaa
    27061 ttgcaaaatc ggaacggcaa aatagaagcc ttcgctctac gagatgcgaa tgataataac
    27121 cgcttgctgg ctgttttgtt tctgaacgcc gccggaaacg gcgttcagac gctttgggat
    27181 atcgatacgg aaaaaattta tataaataga caggctttgc ccgttgaaac ggttgaaacg
    27241 gctaaaaaca ctccatacct taaagctctt tatgatgatt tattgaaaag aggaacatgg
    27301 accgaatcgc cacctataca gaaatatgat tctaatgaca tggcattata acactgcggc
    27361 aggcatactc gactaccgaa cgctggaaaa gccccttatc caaacatcgc gcaaatatat
    27421 gagcgtgacg ccactggatg atgtcacgtc ttggctaaaa taccggcggg tgatcgcgca
    27481 gccggagcaa catgaccagt ctttttccaa aatgtggttt ttgtttcgga tcacggactt
    27541 tgagaaaaac aaagtatatt gccggctggg cgacaaattt attcacgtct atagtcccag
    27601 ttttttcttt attccggcat ttatgcatgc caagtggcat tttcagccgg gtatttacga
    27661 gtatgaagcc tatacatcct acgccgatgt acatgacgaa ctgccggctc gcccgatttt
    27721 tttcccttat gaccgttatc aggatattca aagctatagc gccgtagtaa atcttctaaa
    27781 cggcattaac ggaccagaga tatcgctgcg gcggaagcag tcctatgccg cagagaaaac
    27841 caagttgttt ttagatctgt actatacaga agatgtcggc tttgatgaca tcgccaaaga
    27901 gcttaaactg ccgcgttcta ccatgagccg gtcatttcag aaattctaca ggatcacgcc
    27961 cattgcctac cgcaatattt tgcgaacatg ggaagctatg cgcttgatca gaaacgatat
    28021 aagcgtaacg caagccgcta tggatgccgg tttcaccagc ctaacccaat ataaccagca
    28081 ttttcaccac gttttcgacg cccctccctc ctctttctca taatctcttc tttctgtaag
    28141 cattttacaa aatttcggca aaaatccgaa acttcagcaa aaaccggaag gcgctgctct
    28201 accggtacat gcaatatgga ttgtgtagta atagtagagt atgtagtaat agtagagtat
    28261 gtagtaatag tagagtatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
    28381 tagtaatagt agagtatgta gtaatagtag agtatgtagt aatagtagag tatatggagt
    28441 ttttttatga acgtttccaa aacaaaagag attgcgaaca gcgcgcagac gcgcgatgaa
    28501 gatcttcaca atttaacaat gattttcggt ggacacattt attttcagac gctatatgcg
    28561 gctattaagc tggatctttt cagcatgctt gctgacaaag ggccaatgag tcaggaagag
    28621 atcgccgaag ccttgagcat tgaatcaaag ccagcgcgca ttttgttgtt ggggctgtcc
    28681 tctatcaggc ttctaaaaaa agacggcgac caatattcca atatggctat ttccgacgag
    28741 cttttatgca aaaaatcccc acggaacctg aataagatta ttgaatggga acacgaaatt
    28801 gtttatcagg gcatgtttta tttttatgag tccattaagg ccaataagaa tttgggactc
    28861 gaggttttta aaggaaacga aaaaacgctg tatgagcgcc ttgcgcatgt cgaggagaaa
    28921 aaccagattt tccaggacgc gatgcatcaa atctccgtac aggcaaacgc cgatcttgtc
    28981 caacatgtag atttttccaa tatcggttac cttgtggatg tcggcggcgg cgacggaacc
    29041 aacctgatgc aaatcgccga tagtttcccg cacatccgcg gggcggtttt tgatttgtct
    29101 tctgtttgcc cgatcacaga aaagaatcta aaggataaag gatacagtaa ccgtattgag
    29161 gcaatttcag gagattgttt tcaagacgcg tttcctgaag gcgccgattg tttcctgttc
    29221 tcccatttca tgacgatatg gtcggaggag aaagaccgca cattgttcca aaaagcctac
    29281 gacgcgcttg atgacggtgg aaaggttatg attttcaacc aaatgcagga taatgatcgc
    29341 cagggaccgt tattcgcttc tatcggctcg ccctacttcc tgacaatcgc gacaggcgaa
    29401 gggatgctct atacgtggca ggaatatgaa gactggatga aagatgccgg tttccaggat
    29461 attcaaagct atgaactgcc cagaaatcac ggcgttatta ttggcacgaa atgaaagctc
    29521 accgctgacg cttcaaaaga acatacagcg ccccatcgcc accatgccgc gcctgcgcag
    29581 gtacgctttt aagtacgtat ccttgcaaag acccgctagc caaccattgc ggcacacggc
    29641 gttttaacac cccctgcccg gatgaaagcg gatcgcgccg cccgtcttca ccggccccct
    29701 tccccgtaat caccaaaacg cagcgtctgc cttcacgctg cgcctgcata ataaacactt
    29761 ccaaagcctt atgagcctgc acctgattca tcccgtgcaa atccagccgt ccctcaagcg
    29821 gaatttgacc tttgcgcagt ctatcggccg ttttacgatc cagccccacc cccgcatgat
    29881 cgcgctccga aatctgatcc ggcaattcat ccacctgacg ctgccccgca gaatcaacca
    29941 cccgcctggc cgcagatttt ttatgcgaaa caacaacctt aagcggctta tttcgcgcaa
    30001 tgggctttac atcgcgcgtc acgaaatccc acaaatcctc ttcatcatct tcaaccataa
    30061 tgcgcagtct ataccgcccc ccggcaaaga aaaagtacgg atatggtaca aattcacctt
    30121 tcttgctata atgaaggtgt acaagcatag gaaacaccat gttttcagaa ctatcaaaga
    30181 ttttaccaat caagccccgt caagcggaac acgccgacac acgtcaggat attcagcgcc
    30241 acgaccccga ttatgagcgc cgccacagca aaaaggacgg gccagaagaa caagagccgt
    30301 atgaaaccgg cgccaccgtt gccgtcgaag cgctacaaca ttttttagaa gaatttttgc
    30361 gcaacaactc agaagaaaaa gaacaacccg gtccaaaaaa agaaacgcca cccctccccg
    30421 aaacgcaaaa acaaaatacg gcgctgccgc catcaccaca ggcaaacccg gccgcccgcg
    30481 ccgcctctgc ctatcaaacc atggccatcg caaatgaaaa aagcaccgta ctgctcgaaa
    30541 cgaccgatca agcgggcggc ccgcctctgg acctcagcgc cgccgatatc cgaaccatcc
    30601 atactttgat cgaagattta aaaaatttaa acgatgcagg cgttgaaatc ctccacatcg
    30661 agcgcgccgc cagcttcctc gaaagccttg taaccgccgt gaacaaaatc aaaactacgc
    30721 tctaggatag ccctaacgtg ctggcttccc gctattaagc tgataacctg cctctgtggt
    30781 taataaaatt ctcggcatag ccggatcatc ctcaattttc tgccgaagcc tataaatatg
    30841 agtctctaac gtatgggtct caacaccgtc cacatacccc caaaccctat ctaacaagac
    30901 ctctcgagat accgaccggt cggtatgttc atgtaaaata cgcagcatag aaagctcctt
    30961 ttctgtcaaa cgaacttccc tttctgccac cccttcttta cgcaaagcac tatgagcaac
    31021 atccaaaatc caacccgaaa aatgcaaaag atcctcaccg ccgcgccccg tgtcgacctt
    31081 tcccatccgt ataagccgat caagcaacgc gcccatccgc aacggtttgg ccatatgtac
    31141 ccgcgcccgc gaaccaagaa tcgccgccgc ttccgtcccc agcgcaatac aacgctcagc
    31201 aacagcttca ttatccatca catcctcagg cgcacaaaca tcgacgacgc aaacgccctc
    31261 ataacaagta agcgccctct ggattgcagc acggctcaca gcctgtgaat cagcaatcaa
    31321 aatcggcaat ttctcagcca taactgacaa aaacctcccg gccttttaaa aaacatgaac
    31381 cgccatagta aactcaacat tttgtttttg tgaacacttt tctatagctc atgcttggca
    31441 caacttttgc gaagcttaac gcagacaaag gattttaaat gattgaagga ctttacagcg
    31501 ccctggaaaa tcccgccagc gcacaaacca ccccgccgca aaaagtcctg cgcatggtca
    31561 aaaacgagcg catgggcggt ttcgtgccaa tgtgggaaac gcccaaatcc ggggaaagtt
    31621 ttggcgagca catcgaaaaa tcactctccg aagccctgca aaaaaccggg cccagcgggc
    31681 ctgaaaacgc gctcggctat catggcatgg aaaccagcgc tcaaacatcc gcggacccat
    31741 cgaccaaaga attcggtttt ggagatatta ttgacatggt gaacccgctc caccacctgc
    31801 ccgttgttgg tcatatatac cgcgaactta ccggcgatga aatcaagcca atcggccaga
    31861 tcatgggcgg cgcgctcttt ggcggaccgc tgggcgccgc cagtggatta gtcaatgttg
    31921 ttatacagca ggaaaccggc aaagacgtgg ctggtaatgc ctttgcgctt gtcttaaatg
    31981 gccaggctcc gacctaccgc tcgcaaacac cgccagcaga tagcgccccg gaaattcgcc
    32041 tcgcagcagt gcaaaaacac gcgcaagacc cgcatactgc acaatcaagt gatctgcccg
    32101 gaaaccttct ggcttttgtc gacatgaagg cgcagccaga aatcgtaatt gaacgcacgc
    32161 aagccgcatc tggccgcacc gccggcagcg ttgccaaaac cgcttatcca acgctggatc
    32221 aaaacccgct gccccgccgc gagccaatca cccagatccg cctgagccaa aactacaatg
    32281 attaaatcta tacataccga ccggtcggta tgtattaaaa aagcacctgc aaggctcagg
    32341 acttaacacc taacgcacag aacgctcccc aaacacgccc gttccaacgc gcacatatgt
    32401 tgcgcccaac gcaattgcct tttcaaaatc cacgctcatc cccatactga gctgagaaag
    32461 ccccagcgaa tcggcatatt tcttaagcaa agcaaaatgc agcgccgcag gctcatcaac
    32521 agggggaatg cacatgagcc ccacaatatt gagcccgcac acctcaacgc aaaatcggta
    32581 aaaattctcc aaatccgccg gaacaatccc ggccttttgc tcttcctcgc ctgtattgac
    32641 ctgaataaaa cagggcagat ttttcttatt catatcctcc tgcttggcca tctctttagc
    32701 cagcgcttgc gccagctttt cccggtcaac cgtttcaatc acatcaaaca gtgccacagc
    32761 cgtcttgact ttattggtct gcaacggtcc gattaaatgc agcttcaaat ccggagtaac
    32821 cgcccgccgc ccctgccaac gcgcctgcgc ttcctgcacc cggttctccc caaaaacgcg
    32881 ctggccagcc gccaaagcct catcgatacg cacctcaggc tgcaccttgc tcaccgccac
    32941 caactgcaca tcatccggcg agcgcccggc ttcgcgcgcc gctttcgcga tccggtcatt
    33001 gataatttca agattttcag caacctgccc cataaagcac cttataaccc tttgtattaa
    33061 aattactttt agacttcatc agttcgtttt taatttttat gggggtgccc cgtcccctct
    33121 ggccataacc acaatcagca tgcccgccga caaatgataa taggatatat ttcctattta
    33181 ggcaagggat tacgttgcta ccgtcattgc gaggaatgaa atgacgaagc aatctgcctt
    33241 cccgctctct aaaaagacat aagtggattg cttcgctgcg ctcgcaatga caaaaaacgt
    33301 acaaaagaaa gggcctcaaa cgaggccctt ccaaaatcct tataaaaaat caaactacga
    33361 ttagaagttg atttgtgtac caagcagaac gctcgttgcg tctacttcgt cgcctgatgt
    33421 cagaccggca acattgtcat gctcaacgtg tgatacgctg ccacggaatg tcatgcctgg
    33481 gccgtatgtg tatacgacac cgccagtgta acgatcggtt tcaataccat cattacctgt
    33541 aacaccaaca atattctgcg tgccgtcctg gttcatgtaa gatgcgccaa gcttgaaagg
    33601 accggttgtg tagtcaacac caacaacgag cgtttcttca tcatcgcgtg tgccaccacc
    33661 agtaacttca gtctcaccgt agtcatcttc agagtaagaa acaccgatgc cgaacggacc
    33721 gatatccaga tcgataccag cgttccatac tgtacggtca tcagacgcat cgccagtaag
    33781 caagccagca gttgtgtctt caatgtcaat gtgcgtgtaa ccaagaccgg caatcacacc
    33841 aacattgttg aactgacctt cataacgaag agcagcttcg tatgcagcgc caaagctttc
    33901 ggcaacatca tcagtcagaa cacctgaaag atcaggtgca ccagtatcat gagatgttac
    33961 agctgtaccg ctcacatccg gtgtccaaga cagaccgccc tggaaaccgt tcatgattgg
    34021 agacaggtaa gtcagcttat ctgagtaacc ggttgcattg gtttcatagt caagaccatc
    34081 tgcagtaatc gcagcagcca atgcagctga acccattgaa gtgtagttca caggctgaac
    34141 gaactgacgg ataccgtcaa cgttagagtc tgcagaagga gcggcaacct gcaacaggta
    34201 ttgcgcacca tcttcagcac cgaaattcac acggccccag ccacctgaga agtaaacata
    34261 gctctcttca acatcaaaag agtcacctgt cccaccagcg ccatcagctt ccgcttcgat
    34321 atgcgcacca actgtcagac cgttgtccag agttgtttca ccaccgaagt gaacttccgt
    34381 gtgacgaata acgccaactt cgcgagcatt agccgcagcg gaatcttcgt cttggtctac
    34441 gtatgcaccg tagcctttga accaaccacc aagctcaagt ttaacttgag cagcagccgg
    34501 attagcaatc attgccatac cggcaatagc agctgtgctt aaaagtactt ttttcattat
    34561 cattcctcca ataaacagat gataatcgtt gaacaaaatt gattcgagac tgaatcaaac
    34621 cacccactgt tacccaatgg acacacccaa taagcctgaa tcgcaccatc cgttcaacgt
    34681 gcttttggcg gattgattca tcttttttga aaggcgttgc atttttgcaa caggttaaaa
    34741 cttgctgctc tcgtcattgc gagcgcagcg aagcaatcca tttctaatct ggattgccgc
    34801 gtcagccctg cgggcttcct cgcaatgacg tgggcacaga caatgacgtt aaatggcttg
    34861 agagcaaaag catcccatgc taatcactta caaatatgtt tcacaaaact atgtaggaat
    34921 accatgaacg cgacgcgtaa aacatttctg tccattgcaa ttgtagggat gtcggcactc
    34981 accctaagtg cttgcaccac aggcgaagaa gaaggcggcg caattaaaac cgaagccaaa
    35041 tatccaaccg gtatggcgcg cggcgccaat gaaaatgata tttattcaaa acctgccagc
    35101 atttttggtg aaggtggatt gtcctttggc ggtaaacgca aagataataa tgaagacggc
    35161 gcttccggca ttgccgtgaa cagcttcctc tggcgtgcct cactggatac cgtttccttt
    35221 atgccgctcg caagcgccga tccgtttggt ggcgtgattc taaccgactg gtacacaccg
    35281 gctgaaaccc cggatgagcg ctttaaactc aacgtgttta ttttaagccg ccagctccgc
    35341 tccgacggta ttcaggtccg tgtctttaaa caggtgaaat caaaaggctc atggcaggac
    35401 gttaagccat ccacagaaac cgcccgccaa cttgaagatt cgattctgac ccgcgcccgt
    35461 caattgcgcg tcgcgcagct tgatagtgaa taatttaaca atgtcacccc cgcccatttg
    35521 aaaaatgagt gcgggggtcc ggatgcccgc acaaggcggg catgacagaa ataaaatgat
    35581 cccgaccttc atcatatccc tgtccgaaga aaccgcgcgc cgagaggcaa tcgccgcgca
    35641 cctcaaggat cgcggattca atttcgaatt ttttgatgcc gttgatggcc gccaaatgaa
    35701 tgtgctgaac cacccagatt ataacgctcc gcgccgccgc gccgcccatg gacgcgatct
    35761 caaacccggc gagctgggct gttttctctc ccaccgcaat ttttaccgct ttatggtcaa
    35821 aaacgatatc gactacgcgc tgctccttga agatgacgcc cgcctgcatg aaagcaccaa
    35881 ggccacactt gaagccctca tagataagga tatcgctttt gacatcatcc gcctgctcgg
    35941 cagccccaaa gtggcaaagg gcaaacaccg taaaatcacc cctctatata aagacttcca
    36001 tcttgtccgc ctgcgcactg ctccaggcgg cgcccacgcc acgctcattt cccgccaagg
    36061 cgctgaaaaa ctggtgcgtg ccaccgaaaa attcgccttt ccgattgata cagtgctcgg
    36121 acgcggctgg gaaaccggca taacggcttt ctcgatccaa cccggcctcg ccgtgcagga
    36181 tctctccttt gattccgcca tcggcgaaga gcgccatgat aaaagtgtca cacccaaagg
    36241 cctgaaattc aaatgcacgc gcctcgcctt caaaatcagc gaagcactcg gcaaagcctg
    36301 ggtctattgg tcaacccgcg catcagacct tgcgatcagc aaaaaattca gctaatcgtc
    36361 tctcatgaca caagaaaaat acaatattaa agaatctgaa gcgcggtggc gttccgtttg
    36421 gaacgagcgt aaatgctttg aagtcaccga agatccagcc aaagacaaat actatgtcct
    36481 tgagatgttt ccctatccat ccggacgcat ccatgtcggc catgtccgta actatacgct
    36541 cggcgacgtt gtcgcgcgtt accgccgcgc ctccggatat aatgtcctgc gccccatggg
    36601 ctgggatgcg tttggcctgc ccgcagaaaa cgctgccatt gaacgcggcg cccatccgaa
    36661 agactggacg ctatccaaca catctgagat gcgcg
//
LOCUS       DAOSWR010000005        34219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_824_length_34219_cov_5.319488, whole genome shotgun sequence.
ACCESSION   DAOSWR010000005 DAOSWR010000000
VERSION     DAOSWR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 34219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34219
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_34219_cov_5.319488"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PK513_00805"
     CDS             <1..311
                     /locus_tag="PK513_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81028.1"
                     /translation="NSATATGTLRFAGLDKILSRAQVIGTNINSSPYALPVRELARFL
                     ERLKPLGRVETNAEDGFVHIFNLEMNKKGQFLINGQNASALLQEPKEPMLPAQQKIQP
                     "
     gene            complement(277..1464)
                     /gene="hemW"
                     /locus_tag="PK513_00810"
     CDS             complement(277..1464)
                     /gene="hemW"
                     /locus_tag="PK513_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOO81029.1"
                     /translation="MNIQGQIGVYIHWPFCLSKCPYCDFNSHVHDTIDHVRWQVAYLR
                     ALEHYAEQLRGRTLTSIFFGGGTPSLMMPETVGAIIDKARALWPQVNDLEVTLEANPT
                     SIEGQKFAAFKAAGVNRVSVGVQALNDTDLQFLGRQHSAAEALTAIEIAHKIFDRFSF
                     DLIYARPNQSLEAWRAELDLAVELAGGHLSLYQLIIERNTPFYFDHARGLFALPGEEQ
                     ACSFYNLTQDVLEGAGLPAYEVSNHARAGQESRHNLIYWHYGDYVGIGPGAHGRLSRD
                     DGSRVATREHSAPQVWLDRVEANGYVGHPAQMLSREERFMEAFMMGLRLREGVELKHL
                     SVQGGQDWHAFIDMERLQIVQNEGWVLLDDDKMRLSREGMLRLNALIPYILKAESFVG
                     QAA"
     gene            complement(1486..1887)
                     /locus_tag="PK513_00815"
     CDS             complement(1486..1887)
                     /locus_tag="PK513_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81030.1"
                     /translation="MFKILKNKAVHLQAAFVVGFLTSTTNANAQIGAGHDFSDIARNI
                     TESVEELPGMVTSISYMIGLLMGVLGVMKLKDHVENPSQTPLKDGAIRLAAGGALFAL
                     PIVFESMLNTIGTTGASIEPAALNRAKFNVN"
     gene            complement(2003..2614)
                     /gene="rdgB"
                     /locus_tag="PK513_00820"
     CDS             complement(2003..2614)
                     /gene="rdgB"
                     /locus_tag="PK513_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOO81031.1"
                     /translation="MAREILSLVLATHNAGKAREIADLLGPYISTFYTAGELGLPEPE
                     ETESTFAGNARLKALAAAKASGKPALADDSGLSVTALGGDPGIYSARWAQKPDGTRDF
                     SYAMNKVEEALGTAEDRSAAFVCALCLAWPDGHVEIFEGRAEGHLEFPPRGTHGFGYD
                     PIFVPQGQTITFAQMEPDQKHAMSHRADAFRQLVAATFEDEAV"
     gene            complement(2699..3058)
                     /locus_tag="PK513_00825"
     CDS             complement(2699..3058)
                     /locus_tag="PK513_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81032.1"
                     /translation="MKIFFSWRNVMLLNSDETKELSNMSIAFLAAMAIGNEDEANSLL
                     VKDVEGEISQAVKRIGFFVGFGGHSPAVLPVQLLKELEGDSVESLMNFGNDFSLMREE
                     DQRCVNNLISCAPCFMS"
     gene            complement(3094..3813)
                     /gene="rph"
                     /locus_tag="PK513_00830"
     CDS             complement(3094..3813)
                     /gene="rph"
                     /locus_tag="PK513_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOO81033.1"
                     /translation="MARPSGRAFDEMRDVEILPHVNKHAEGSCLIKFGDTHVLCLATA
                     EERVPGWMKGTGTGWVTGEYGMLPRSTGLRMDREAAKGKQSGRTQEIQRLIGRALRSV
                     VDFEKMGERQVRVDCDVLQADGGTRTAAITGGFVAMHLAFQHWVETGAMASVPINDTV
                     SAVSCGIYEGAGISDLDYDEDSAAQTDANFVMTGSGGIVEIQGTAEKEPFSEEEFLKL
                     LELAKKGCAELTAVQKDVLGL"
     gene            3975..5039
                     /locus_tag="PK513_00835"
     CDS             3975..5039
                     /locus_tag="PK513_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81034.1"
                     /translation="MLIAIGLKTMKKLLTLALLLCFLHTGFSPDANAQLSFDRDDNVK
                     NYNNDNNSDSDEIDFDFDFSGDGEKDEGPKVINPFDDKYKPDLTAEETEGVNLTPNYF
                     YYFTILRKPDASPDDINLFIFSPGTIVGCLSKEPASLETVKFSPTMQIKLTEGQTDVD
                     RETVRYFHYECKPETGMVEMYITLSKKQLIKDKIKKLVLLSEAVGPFSDIIIDFHDNH
                     IDIESKLHDLGCFGLKTHGIPKKFTYWLYPENTMVLFSSSADIKEKKTRDKVIALARR
                     HGLTPLDEIIPEFEKSHYHDDKLFVVDTAGNYKDKLPSIRDRFAFGKIQDSETYFGAK
                     GPYTKVTEKMIYARSPDIHE"
     gene            5135..5854
                     /gene="grpE"
                     /locus_tag="PK513_00840"
     CDS             5135..5854
                     /gene="grpE"
                     /locus_tag="PK513_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO81035.1"
                     /translation="MNTPTPEKPATSEREETLAERSARLESEAAGLYNKDEQDPLAPE
                     AIAETDEERQDAPATASGVELVELKALQEELAATRDQAKRALAEAENTRRRAIKERED
                     ASKFAVSGFAKDLLDVSDNLRRALEAVPTDLLESAPRLKNLVDGIEATERTLLRTFEK
                     HGIEKIEPLDQPFDPNYHEVMFEAPVPGKPAGTVMQVMESGYVLNGRILRPARVGVVK
                     DDGTTPPTGETPPGGHIDTQA"
     gene            7056..8993
                     /gene="dnaK"
                     /locus_tag="PK513_00845"
     CDS             7056..8993
                     /gene="dnaK"
                     /locus_tag="PK513_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOO81036.1"
                     /translation="MSKIIGIDLGTTNSCVAVMDGKETRIIENAEGARTTPSMIAFSK
                     DGERLVGQPAKRQAVTNPENTLYAIKRLIGRPFKDKQVQDMASKAPFKIIEGENGDAW
                     VEVNGEKYAPSQISAMLLQKMKETAENFLGEKVTKAVITVPAYFNDSQRQATKDAGKI
                     AGLEVERIINEPTAAALAYGLDKKEHGTIVVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTFLGGEDFDLRIIDYLADEFKKSDSIDLRKDKLALQRLKEAAEKAKIELSSTTQTEV
                     NLPFVTADANGPKHLQVSLSRAKLESLVEDLIKRSIDPVKKALKDAGLKPADIDDIIL
                     VGGMTRMPKVIETVKNYFGKDPHKGVNPDEVVADGAAIQGGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIERNTTIPTKKSQVFSTAEDSQNAVTIRVFQGEREMAADNKILG
                     QFDLVGIPPAPRGIPQIEVTFDIDANGIVNVSAKDKATNKEQQIRIQASGGLSDADID
                     QMVKDAESHADEDKKKRETVEAKNVGESMIHATEKSLEELGGDLPNDEKNKVEQAIQD
                     LKDVLESGNAEDIKAKTESLSQASMKLGELAYRKAQEEAAGEAETQEAEPVDADEDEV
                     IEADFTDLEIEDEDGKDQKSA"
     gene            9108..9419
                     /locus_tag="PK513_00850"
     CDS             9108..9419
                     /locus_tag="PK513_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOO81037.1"
                     /translation="MKNNTSYYEILNISPKASDDEIRRAYHTLAKKFHPDRNPQNRRL
                     AELRFRVISEAYANLKTREKRAAYNRTRRLQAQNDNKNTGNFFSQISEIFKPKNKEIK
                     N"
     gene            9419..10576
                     /gene="dnaJ"
                     /locus_tag="PK513_00855"
     CDS             9419..10576
                     /gene="dnaJ"
                     /locus_tag="PK513_00855"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO81038.1"
                     /translation="MPNDLYKTLGVDKTASADELKKAYRKLAMKYHPDQNKDNAEAEA
                     KFKEVSHAYDILKDEQKRAAYDRYGEAAFDGSMGTGGAGGPSGFGANAGAFSDIFEDM
                     FGEFMGGRAGAGRSGKARGSDIQYTLELTLEDAYKGKDAQIKIPVNDTCEACKGSGAQ
                     SGTSAGDCSTCDGQGRVRQQQGFFTIERTCPTCHGAGKIITDPCKKCHGQGRIKKDKT
                     LKVKIPPGVDTGRRIRLSGEGEAGVQGGPRGDLYVLLAIKPHKLFKRDGANLFCRVPI
                     TVTKAALGGDLEVPTIEGKATKVKIPPGTQTGQQFRLKGKGMPILGSDSKGDLYIETF
                     VETPVNLNKKQQDILKQLDKDFSENNGGGKHSPEASGFFTKVREFWDDLKD"
     gene            10591..10998
                     /locus_tag="PK513_00860"
     CDS             10591..10998
                     /locus_tag="PK513_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81039.1"
                     /translation="MALDTLITPLNFNDAIIGKSINDKKSGLDIIVGYISGMPPDLAE
                     MVEQFDSIGLALMNRGIGQHELDQACQKLAEQCQNEISSLLITKSDLPFDARWYLIGD
                     LLQMLELARKDIISLPFSDFLYDELSDFLRTYR"
     gene            11051..11821
                     /gene="dapB"
                     /locus_tag="PK513_00865"
     CDS             11051..11821
                     /gene="dapB"
                     /locus_tag="PK513_00865"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOO81040.1"
                     /translation="MKIGVTGCTGRVGSLITKELLSGDWKELELAGGTVLNEDEITDD
                     FFMTTDAEDLFARADTIIDFTSPRAVPTHTELAAKHGTILIIGTSGLSEADEALVKQA
                     ATKTPVVYAANMSVGVNLLIALVEQAARRLDDSWDAEILDLHHKYKVDAPSGTSYALA
                     KAIQKGRGKAFDKQDLTLAREGHTGAREAGKIGFSVQRGGDVVAENSTIFFGMGERLE
                     ITHKATDRAIFARGALRAALWAKGRPAGLYSMNDVLGL"
     gene            complement(11831..12331)
                     /locus_tag="PK513_00870"
     CDS             complement(11831..12331)
                     /locus_tag="PK513_00870"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOO81041.1"
                     /translation="MEKIIYGFYPSSLGEMVLGKTEKGLCWLGFMVAGYKGDGLERMR
                     AHFKGAEFVHDDDELRDLGDQVMEAWAQGRENEIALDLHGSVFQKAVWKALLKIRKGQ
                     KLCYGDVANDIGRPAAARAVGSAVGENPVSLIVPCHRVVQKSGALGNYGWGADLKRKI
                     LRMEKL"
     gene            12365..13009
                     /gene="nth"
                     /locus_tag="PK513_00875"
     CDS             12365..13009
                     /gene="nth"
                     /locus_tag="PK513_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO81042.1"
                     /translation="MTLSQKNIQLFFSRLRKIQPEPKSDLKWTNAYTLLVAVTLSAQA
                     TDAGVNKATHELFKIADTPQKMLGLGIDGLKEHIKTIGLYNNKAKNIIAMAEILVNEY
                     DGQVPDSRDKLMKLPGVGRKTANVVLNIVFKQPTMAVDTHIFRVANRTGMAPGKTPEA
                     VEQALLKAVPDEFALHAHHWLILHGRYTCKARKPDCPSCIVADLCEFKEKTHPD"
     gene            complement(13040..13993)
                     /locus_tag="PK513_00880"
     CDS             complement(13040..13993)
                     /locus_tag="PK513_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81043.1"
                     /translation="MKFLLLLFLFLIAYTPARACRYIPQDFETLYNQADVVFIGVVQS
                     LKESEVVFNIEKGYKNASAGNVFTVQPGSSSCHVRFSPGQKWLYLGDTVMTGSRILQY
                     ENGMENPENIAFVDSFFAHRDDSLVGQIDLDVFAGWPPKGRWRACDADQDCRLMYIGC
                     NSLLAVSQDHYKAAEDYYYNKRGLDPRAMDCAVSAPGEQSEKYAGEIMLKCVQGKCGA
                     WQHETCLNPPACTQIYRNHCTVELKSYMDPENEDSYGSGLRILFAMSETQCADKCRKE
                     FEAQQGWARQYYIPKVGAQCLFNDILLFDETVLIAPHETEN"
     gene            complement(14047..14547)
                     /locus_tag="PK513_00885"
     CDS             complement(14047..14547)
                     /locus_tag="PK513_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81044.1"
                     /translation="MYQSKLNLLIVLFVIVGFFFAIHSLKQDYGQAQAASGLAGKMIV
                     RDAEYSRADKRALRRVMLGGSESLLSLSARDVRVVLSQPELVRRDLPTVIWQYRNKVC
                     VLDVYFTVADGVKKVSEAPVAHYEVRARQKGMSDQDVQEECLENLVRANAEARFVRLD
                     GFYKSN"
     gene            complement(14773..15579)
                     /locus_tag="PK513_00890"
     CDS             complement(14773..15579)
                     /locus_tag="PK513_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOO81045.1"
                     /translation="MFHRVKSEDQEPEKVSAPTTDEQDAQRQTRDDITEFKGPDSDEK
                     AEPKSSTSTVETPQEEDQGTMTDQDKTATEQEGSAPAVMGAYQRPGQAPVRAAGGYPG
                     AYPGLGGSPADTGSDRRLTIGSGITMSGEIESCDYLLVEGTVEAALKGANVLEIAESG
                     TFYGTVEINEANIAGRFEGDITVSGRLTVSSTGTVTGSISYKEIALEAGAIIDGKLTP
                     LSAAPSAAKKPSAAPKVTKSAVPKVEKVERQPEPANNDEGLFSKAAAAAE"
     gene            15834..16625
                     /locus_tag="PK513_00895"
     CDS             15834..16625
                     /locus_tag="PK513_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOO81046.1"
                     /translation="MAKSGFSFLSLLGITDSPESSPESLPKNAANNHVYDVQIVPGES
                     KAESTAKITVDKGAVEQIEGDTLEDKAQAIEKELNEANQQYDIAGPAEYDVAIVCDDT
                     SPQSTAPVAPAAPVEIKHFRQEESVMGIQSYLIGRGHERVGDKHNNPDGMFGNGSNKA
                     LAEEIKSFQQQNGIEPTGEYNDATRQAYLTQAAKMEGGSAGRDALEGFVEGLDAMKNK
                     DIDGHSALDRVYHPEQAVEQAPQAGMCKITIDTSILDSTFSVKPT"
     gene            complement(16646..18505)
                     /gene="uvrC"
                     /locus_tag="PK513_00900"
     CDS             complement(16646..18505)
                     /gene="uvrC"
                     /locus_tag="PK513_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOO81047.1"
                     /translation="MDDVVKISPVELGARIIKGYVKKLPDTPGVYRMLCEGGEVLYVG
                     KAKSLKKRVVNYTRPETLPIRLQRMISITRAMEFVHTHTEAEALLLESNLIKKFNPKY
                     NVLLRDDKSFPYILMTEDHDFPQVVKHRGAKKRKGQYYGPFASAGDVNRTIGVLQRVF
                     LLRNCSDGYFAQRTRPCLQYHIKRCTAPCVGKVSKKEYARQIMEAQDFLDGKSQAVQT
                     RMVEKMQTACSAQNYEAAAVYRDRIQALSSIHARQDINVAGLGNVDVMALAQKEGRSC
                     VQVFFFRGGRNYGNWSYFPRHDRDEEPANVMAAFMAQFYENKLVPKNVLVNVLPEEKA
                     LLEEAFSSKVSHKVVISKPQRGGRKRIVDFALRNAREALARNLVEKAGEAKLLAGVAD
                     LFGMDELPKRIEVYDNSHISGTNMVGGMIVAGPDGFNTGAYRKFNIKRAEAGDDYGMM
                     REVLERRFKRALKEAPEMSGPNWPDLLLIDGGKGQLSACAEVLEDYGVLDALTLVAIS
                     KGPDRNAGREQFHMVGRASFQLPVNDPVLHYLQRLRDEAHRFAIGTHRARRQKQINAS
                     PLDGVPGVGGKRKKALLNYFGSAKAVAGAGVEDLQKVEGISESLAQVIYDYFH"
     gene            18702..19319
                     /locus_tag="PK513_00905"
     CDS             18702..19319
                     /locus_tag="PK513_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOO81048.1"
                     /translation="MQHHQIRSTVDNTVLFEGRFENFKACLEQAVADRVALHCVNLRN
                     QNLTNANLDDALMPGADFSGTNLTGANMSESYLKGADFKSTVLYNTCFYDSNLSACNF
                     EGAAFGATDIHGTILSHAQFSTLSCFCLDFVRARAMSGCIFINPDGRICEMSKPPIVV
                     RGAGNAPIILLDNKIKSGHNIIDHKRLRPLIEKLSVRTLRKRIAT"
     gene            19407..19492
                     /locus_tag="PK513_00910"
     tRNA            19407..19492
                     /locus_tag="PK513_00910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19440..19442,aa:Leu,seq:taa)
     gene            complement(19510..20271)
                     /locus_tag="PK513_00915"
     CDS             complement(19510..20271)
                     /locus_tag="PK513_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO81049.1"
                     /translation="MGQRSSLVVIGNEILSGRTSDKNINWIAEHMTKHGVPLAEVRVV
                     PDIEEKIIEAVNVLRDEYDYVFTTGGIGPTHDDITATSIAKAMGVVFERHAEAYHVLL
                     DYYGPDELNETRARMANMPRGARLLLNPVSGAPGFAIENVFVMAGVPRIMRAMLEHVI
                     AEIKPGAAILSNTVFCDLKESIVAEGLREIQGRYETVDIGSYPSFRGGALNVSVVLRG
                     TDEATIRAATKEVLALVERCGGGAQSLSFQVPVDD"
     gene            20362..21192
                     /gene="map"
                     /locus_tag="PK513_00920"
     CDS             20362..21192
                     /gene="map"
                     /locus_tag="PK513_00920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOO81050.1"
                     /translation="MNTANTYTSEGIELHADNADFEGMRRAGKLAATILDEVTPLVVP
                     GVTTEELDKFCHEYALKHNAIPAPLGYGEGPGRPPFPKATCTSVNHVVCHGIPNEKTL
                     REGDIVNIDVTVILDGWHGDTSRMYMVGNKHSVKAKRLVDVTYDAMMRGIEAVKPGAT
                     LGDVGYAIQSYAESERFGVVTDFCGHGLGKVFHAPPSVMHIGQPGKGTVLEPGMFFTI
                     EPMINAGDYRTKVLPDGWTAITKDRSLSAQFEHSLAVTDDGFEIFTLSPKGYTKPPYE
                     "
     gene            21452..22144
                     /gene="radC"
                     /locus_tag="PK513_00925"
     CDS             21452..22144
                     /gene="radC"
                     /locus_tag="PK513_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOO81051.1"
                     /translation="MTAAQKAQKPHYRGHRDRLRERFAKTPDALADYELLELVLFLAI
                     PQKDVKPLAKDLLKRFGSLSEIMHAPIEELTRVEGIKDKTALALKSITALSNRAAKNE
                     LINKPVLNSWTRLMDYCHATMAHETREHFRILFLNKKNELIADEIQGSGTVDHTPAYP
                     REIMKRALELGATALILMHNHPSGDPKPSQADVDMTTQIISAGQPFNIVIHDHIVMSK
                     NGYSSFKNMGLL"
     gene            22252..22587
                     /locus_tag="PK513_00930"
     CDS             22252..22587
                     /locus_tag="PK513_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81052.1"
                     /translation="MTILACLILSACAPQLPTSEKLEQIRLAKQDALATEITASGLTL
                     GQPTYIRVFKSENLVETWVQDETTGRYAPFKTYPICNYSGLLGPKLMEGDRQAPEGFY
                     TVTARQMNP"
     gene            22642..22980
                     /locus_tag="PK513_00935"
     CDS             22642..22980
                     /locus_tag="PK513_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81053.1"
                     /translation="MGHTGSNLMIHGDCKSDGCYALTDPAIEEVYLLTEASIREGHPV
                     PVHIFPFRMHARNMAQHNASPWSPFWQNLREGYDAFEITRIPPEIRVQTDLTGAMYIF
                     EEQNFKTALY"
     gene            22994..23278
                     /locus_tag="PK513_00940"
     CDS             22994..23278
                     /locus_tag="PK513_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81054.1"
                     /translation="MSLSDKNPSIDRFFDPVINCTSHNELRTWLKKQNEEDIRTFLDT
                     YNFDTNQAEKSILRQELERRSSIKNQKDKWIDRIIGFVFGILSGFLINSF"
     gene            23424..24734
                     /gene="purB"
                     /locus_tag="PK513_00945"
     CDS             23424..24734
                     /gene="purB"
                     /locus_tag="PK513_00945"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOO81055.1"
                     /translation="MIPRYSRPEMANIFEQENKFSIWLEVETLALEKMADLGIVPESA
                     AIALRGKGDFDIARIDEIEAEVKHDVIAFLTSVAEHVGEESRFVHQGMTSSDVIDTSF
                     AVMLTQAADLIIEDLDKLLAALKKQALAHKDTLCIGRSHGIQGEPTTFGLKMAGHYAA
                     FARAKKRMIAAKEEISTITISGAVGTYPVLPPEVEQYVADKLGLKPETVATQVIPRDR
                     HAMFFATLGVIASSIENLAVEIRHLQRTEVREAEEYFDPGQKGSSAMPHKRNPIGSEN
                     MTGQARIIRAAVIPALENVALWHERDISHSSVERTILPDALISADYAINRLAGIIDRL
                     LVYPERMIHNLEMTGGLLFSQRVMLALTQEGISREDAYRIVQKNAMAVWEDRANGKGS
                     KTLRGELENDAAVTDKLDSAAFEAIFNYDAYTKNIESIINRALA"
     gene            24746..25237
                     /gene="bfr"
                     /locus_tag="PK513_00950"
     CDS             24746..25237
                     /gene="bfr"
                     /locus_tag="PK513_00950"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018148183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOO81056.1"
                     /translation="MKGDKKVIQYLNDALKKELGAINQYFLHSRILEDWGITKLAKHE
                     YNESIEEMHHADELIKRILFLGGLPNLQEVAKLYIGETVKETLECDLKIELEGVTGYK
                     EGIKYCREVDDFVTEDLFKKILAEEEGHVDYLETQLEMIQQMGLENYIQINSQSPLDT
                     EGE"
     gene            complement(25394..25549)
                     /locus_tag="PK513_00955"
     CDS             complement(25394..25549)
                     /locus_tag="PK513_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81057.1"
                     /translation="MIFDDVFEGGETLAAFGVFAAGAGAVGVTDTGTFSVAVEMVFNL
                     FIVEGVT"
     gene            25716..26204
                     /locus_tag="PK513_00960"
                     /pseudo
     CDS             25716..26204
                     /locus_tag="PK513_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            26349..27044
                     /locus_tag="PK513_00965"
     CDS             26349..27044
                     /locus_tag="PK513_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002127187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOO81058.1"
                     /translation="MKTIGILGGMSWVSSLHYYKWLNELAQDHLGPINNSKILLNSVD
                     WQKIQKFQKDDDWNDAGNYLAAEARKLESIGAELMLIGTNTMHKVADQIQAAINIPLL
                     HIADAAAMRIKAQGLNTIALLGTTFTMEQDFYKSRLENSGIKVLIPENQNDRDKINAI
                     IFDELCQNVKKTESKTEYIRITQEMLDQGAQGLIMGCTEITTLIGADDFDKPVFDTTR
                     IHVEEAFELAQEN"
     gene            27048..28667
                     /locus_tag="PK513_00970"
     CDS             27048..28667
                     /locus_tag="PK513_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha glucosidase"
                     /protein_id="HOO81059.1"
                     /translation="MSNNWWRGAAIYQIYPRSFFDTNGDGIGDLPGITEKLDYIASLG
                     VDGIWISPFFTSPMKDFGYDVSDYHDVDPMFGSLEDFKTMLVKAHKLNLKVIIDLVLS
                     HTSDQHPWFKDPPKKDWYIWADPRPDMFGERVPPNNWVSVFGGPAWTYDENRGQYYLH
                     NFLPEQPDLNFHNPDVQNAMLDVCKFWLYIGVDGFRLDVVNFYFHDRELRDNPPRDAD
                     LGPATQYEGDDPYSAQNHIYDKTRPENPIFIQRVRALIDQYPERIIIGEGGDDHFFKL
                     AADYCSGDKRLHTIYSPQLCAGAHKRLTADLIRDPVENFLNQAPGAWPTWAFSNHDVV
                     RAASRWCDKGEGFDHNPRLSKTLIALLGCLQGSFFLYQGEELGLPEAKLKFEDLQDPW
                     GKHLWPDWQGRDGCRTPLPWNKMPQAGFTTGKPWLPMAETHLPLNIEKQDSDPTSALN
                     FTRKFLSWRKNQPALISGTITFLETDSDTLLALERKNKGQTLICIFNLENASQTLPPF
                     LLPDNIETTFPTKNQSTAPMAPYDFQIWRYT"
     gene            complement(28664..29503)
                     /locus_tag="PK513_00975"
     CDS             complement(28664..29503)
                     /locus_tag="PK513_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81060.1"
                     /translation="MSIWFVLWFFISAALLGFLGWSLLILYQQKKTWKAFAAKHKLRF
                     KPNSMMEGAEMEGNIDSYKVSFFTSEHLNPDMRSSRKLTAVEVGLHSTMPIDGAIASG
                     QMISFIKTLNFKMEVRPKHKDWSLEYLVVGDNRFVLDAYLTDERIELLVKLMKIKNSC
                     MVLIFKGDRMLLRVDMADPMISAEYLDRFVKLLLKAAKTLEVSSDEAKKLKAEEVRGV
                     GKDSSLVLDDTVEAGASGLQLEDDDLDAGDGEESEPQPQKEETEPVEKTQKSSQKKAK
                     PRD"
     gene            29986..30651
                     /locus_tag="PK513_00980"
     CDS             29986..30651
                     /locus_tag="PK513_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81061.1"
                     /translation="MISIEQCRGARGILGWTQQNLADASGLSKTAINNFEKGHSDIKA
                     ESLRAIRMAFESAEMEFLGQNGIRKKTEDVRILHGPESMSILLEDIQSTMAARNGEIL
                     IMNANNNLSSQVPTQKLFDHIDLIKTNHIRQRILCPEGTKNILSPNDECRWVPADIGQ
                     RCILTFIYTGKVAFQLWDQSIIIIIDSNIAHESEKKRFEHIWANAMPSTEQKSNASKQ
                     VQT"
     gene            complement(30731..31381)
                     /locus_tag="PK513_00985"
     CDS             complement(30731..31381)
                     /locus_tag="PK513_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81062.1"
                     /translation="MTMDVETIVAVSLMMAVFSAVAAVGTSLVLGAGFERLRNGFEII
                     SKQTGFFSDAIHKLEQKVDVVDGQASKFSESITALDAKVNNVGEQANVFSDTVAKLDK
                     KVSVVDQQTGFFSDAIHKLEKKVDLVSGHEEIVAEKMEKSAAHDLISTGKAEALVAHA
                     EDLLSQVGDLASKIQIQQEIQNIPVSGQALHLNMPRNLQQFSLQSGFADQEEVHYH"
     gene            complement(31771..32076)
                     /locus_tag="PK513_00990"
     CDS             complement(31771..32076)
                     /locus_tag="PK513_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81063.1"
                     /translation="MKTARALLLVSPFLLGACGEGFELVQTDTMFPYGNQRTAGSGHA
                     YVLAKMLPKKEMKLQPVAARPAPQPLQETKDIIEDIPAPVEPQPQKAEEIFREQQEK"
     gene            complement(32242..32787)
                     /locus_tag="PK513_00995"
     CDS             complement(32242..32787)
                     /locus_tag="PK513_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81064.1"
                     /translation="MNIYIETDRLLLRDWKEEDRAPFARLNADPLVMEYMPRPLDEKA
                     SNKLVGHFEDHLARKGYGLFAVEVKESGQFAGFVGLDTVRFTAHFTSPKKPATEIAWR
                     LDYEFWGKGYGSEAASAVLDYGLTDLKLKEIVGFTVYDNTRSIQLMEKIGMVRDEDGD
                     FAYPTLRKGHPMGHLVLYRAS"
     gene            32941..33642
                     /locus_tag="PK513_01000"
     CDS             32941..33642
                     /locus_tag="PK513_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81065.1"
                     /translation="MKFLLALSCAATLSLTAIHSFANDMPLPERKPSYTNVNADHKTL
                     SKAKFLLEQISIELKKPVEKVSAKPTPEPPPTTTPPQDTQPYELTENLQISRQGDTNM
                     HCGALSSEAMRMRDVIYATQEIKAKSRIQSHAVTAAGAIGSLLIGSVTGGIGLAVGGF
                     LLDHNVGHNANEADNIQDIAEQRRTLMMGIYNAKGCEGPLDHAMQNPEIFDPLARIAS
                     IESAAGDIDGHDFND"
     gene            33720..34085
                     /locus_tag="PK513_01005"
     CDS             33720..34085
                     /locus_tag="PK513_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81066.1"
                     /translation="MKRTAIALSYLAALTLPTASQAQSFLDRIDNAVSKVENTIARTE
                     RTITRTENTAERLNDKIPEAGAETPAEEIPAQEQTSANNGVTAEDQRILDQAKKIEEE
                     RVLREAEKIRTRRTNTNGR"
BASE COUNT         9886 a        10031 c         6788 g         7514 t
ORIGIN      
        1 taaacagcgc cacagcgacc ggaacactgc gttttgccgg actggataaa atcctctccc
       61 gcgcacaagt catcggcaca aacattaaca gctcacctta cgcattgccc gtgcgcgagt
      121 tggcacgttt tctcgaacgc ctaaaaccat tgggccgcgt tgaaaccaat gccgaagatg
      181 gcttcgtcca catctttaat ttagaaatga ataaaaaggg acaatttctg attaacggcc
      241 aaaacgccag cgcactttta caagagccca aagaacctat gctgcctgcc caacaaaaga
      301 ttcagcctta agaatataag gaatgagcgc attaagccgc aacatgccct cacggctcaa
      361 tcgcatttta tcatcatcca acaaaaccca accctcattt tgaacaatct gcaaccgttc
      421 catatcaata aatgcgtgcc aatcctgccc gccctgcaca cttaaatgct taagctccac
      481 accttcacgc agccgcaaac ccatcataaa cgcttccata aaccgctctt cgcgcgaaag
      541 catctgcgcc ggatgcccca cgtatccatt cgcctcaacc cgatccagcc agacctgcgg
      601 cgcactatgc tcacgcgttg ccaccctgct accatcatcg cggcttaaac gcccgtgcgc
      661 ccccggacca ataccaacat aatcaccata atgccaataa atcagattat gccgcgattc
      721 ttgccccgcc cgcgcatggt ttgacacctc ataggcgggc agtccagccc cttccaaaac
      781 atcctgcgtt aaattgtaaa acgagcacgc ctgctcttca cccggcaaag caaacaaccc
      841 gcgcgcatga tcaaaataaa acggcgtatt gcgctcaatt ataagctgat ataaggacaa
      901 atgcccacca gccagttcca ccgccagatc aagttccgcc cgccaagcct ccaaggattg
      961 attgggccgc gcatatatca aatcaaagct gaaacgatca aaaattttgt gcgcaatttc
     1021 aatcgccgtc agcgcctccg ctgcgctatg ctggcggcct agaaattgca aatccgtatc
     1081 attaagcgcc tgcaccccga cagatacgcg gttcacaccc gccgctttaa atgccgcaaa
     1141 cttctgccct tcaatcgagg ttggatttgc ctccagcgta acctccagat cattgacctg
     1201 cggccacaac gcccgtgcct tatcaataat cgcgcccaca gtttccggca tcatcaacga
     1261 cggtgtcccg ccgccaaaga aaatcgaggt taacgttcgc ccgcgcaatt gctccgcata
     1321 atgctccagc gcccgcaaat acgccacctg ccagcgcaca tgatcaatcg tgtcatgcac
     1381 atgggaatta aaatcacaat acggacactt ggacaaacaa aaaggccagt ggatatacac
     1441 tccaatctgg ccttgaatgt tcatatttaa aacaatccgt taaacttaat taacgttaaa
     1501 cttcgctctg ttgagcgctg ccggctcaat agatgccccg gttgtcccaa tcgtattaag
     1561 catcgattca aacacgatag gtaaagcaaa gagcgctcca ccggcggcca aacgaatagc
     1621 cccatccttc aatggagtct gcgatgggtt ttcaacgtga tctttaagtt tcatcacacc
     1681 caaaacaccc attaacaaac caatcatata agaaatggat gtaaccatcc ctggcaattc
     1741 ttcaaccgac tcggtaatat tccgggcaat atcactaaaa tcatgccccg cgccaatctg
     1801 cgcattcgca ttcgtcgtac ttgtcaaaaa accaacaaca aaagcagcct gcaagtgaac
     1861 agctttattt tttaaaatct taaacattac aacctccttg aatacgcttt aacaccttgc
     1921 atattgcagc gattttatat aataaaaatc gcccaaaagt caacttttca gcaaatccca
     1981 ataaaatctt atgggcgtaa ccctacaccg cttcgtcctc aaaagtcgca gcaacaagct
     2041 ggcgaaaagc gtcagcgcgg tgcgacattg catgcttctg atccggttcc atctgtgcaa
     2101 aagttatcgt ctgcccctga gggacaaaaa taggatcata cccaaagcca tgcgttccac
     2161 gcgggggaaa ttccagatga ccttcggccc gcccttcaaa aatctcaaca tgtccgtctg
     2221 gccaggccaa acaaagtgcg caaacaaatg ctgccgatct gtcctcagcc gtccccagag
     2281 cctcttcaac cttattcatc gcatagctaa aatcgcgcgt tccatccggt ttttgcgccc
     2341 agcgcgccga ataaatcccc ggatcaccgc ccagcgccgt aacgctcaaa ccactatcat
     2401 ccgccagcgc cggcttccct gaagccttcg ccgctgccaa agccttcaac cgtgcattcc
     2461 cggcaaacgt actttccgtt tcttccggtt caggcaagcc caactccccg gcagtgtaaa
     2521 atgtcgaaat ataaggcccc agcaaatccg caatctcacg cgccttaccc gcattatgcg
     2581 ttgcaagaac aagagataaa atttcacgtg ccactgtcat tcctcatcgt acataaatct
     2641 aatctaaaat caggatagaa actcagcagc atacagtaaa gtaagagaac actaaaaact
     2701 aagacataaa acaaggagca caacttatta aattatttac acaacgctga tcttcctctc
     2761 tcataagaga aaagtcatta ccaaaattca tcagagattc aacggaatcg ccttccagct
     2821 cttttagtaa ttgcacgggc aaaaccgccg gggaatgccc tccaaagcca acaaaaaagc
     2881 ctattctttt aacagcttga ctaatttcac cctccacatc tttcactaac aaactatttg
     2941 cttcgtcttc atttcctata gccatagcag ctaaaaaagc aatagacata ttagaaagtt
     3001 ctttcgtctc atctgagttc aataacataa catttctcca actaaaaaat attttcataa
     3061 cgcaaaaatc gttcgcctgc aagaacaaac gatctaaagc cccaaaacat ctttctgaac
     3121 cgccgtcaat tctgcacacc ccttcttggc caattccagc aatttcaaaa actcttcttc
     3181 actaaacggc tctttttccg cagtcccttg aatttcgaca attccgccac tacccgtcat
     3241 cacgaaattc gcatccgttt gcgctgcgct atcctcatca taatcaagat ccgaaatacc
     3301 cgcgccttca taaatcccgc acgaaaccgc cgagactgta tcgttaatcg gtacagaagc
     3361 catcgcgccg gtttccaccc aatgctgaaa cgccagatgc attgccacaa acccgccggt
     3421 aatcgccgcc gtacgcgttc caccgtcagc ctgcaacaca tcacaatcca cgcgcacctg
     3481 ccgctcgccc atcttttcaa aatccacgac ggaacgcagc gcccggccaa tcaaacgctg
     3541 aatttcctgc gtccgtcctg attgcttgcc ttttgcagct tcccggtcca ttcgtaagcc
     3601 ggtcgaacgt ggcaacatgc catattctcc cgttacccag cccgtgcccg tgcctttcat
     3661 ccagcccggc acacgctcct ctgccgtagc cagacacagc acatgcgtat ctccaaattt
     3721 aatcaggcaa gagccttctg cgtgcttgtt cacatgtgga agtatttcaa catcgcgcat
     3781 ttcatcaaat gctctgccag atggtcgggc cataaataaa ctccaagatt aagtgtaatt
     3841 aactgtcgcc agactaagca tcacatcctc ccatctcaag aactttattc tacaaaggtc
     3901 gtggatgaac ctgcattcag attgctgaaa cccttgcctg cctcaagcat tcttggcacg
     3961 ataaaggctc aaaattgctg atagccatcg gattaaagac catgaaaaaa ctgctcaccc
     4021 ttgccctgct cctgtgtttc ctacacactg gattttcccc tgacgccaac gctcaactaa
     4081 gctttgaccg cgatgataac gtcaaaaact acaataacga taacaacagc gacagcgacg
     4141 aaattgattt cgacttcgat ttcagcggcg atggtgaaaa agacgaaggc cctaaagtca
     4201 tcaacccctt tgatgataaa tacaaaccgg acctgacagc cgaagaaacg gaaggcgtca
     4261 atctaacccc caactacttt tattatttca caatacttcg caaaccggat gcttccccgg
     4321 atgatataaa cctgttcatc ttttctcccg gcaccattgt cggctgcctg agcaaagaac
     4381 cggcaagcct ggaaacagtt aaattcagcc cgaccatgca aatcaaactc acagaagggc
     4441 aaactgatgt tgatcgcgaa accgttcgct actttcacta tgaatgcaag cccgaaaccg
     4501 gcatggtcga aatgtatatc acgctcagca aaaagcagct aatcaaagac aaaatcaaaa
     4561 aactcgttct gttaagtgaa gccgtcggcc ccttcagtga tattattatt gatttccatg
     4621 ataaccatat cgacatagaa tcaaaactcc acgacctcgg ctgcttcgga ctaaaaactc
     4681 acggcatacc gaaaaaattc acatactggc tttacccgga aaataccatg gtcctgtttt
     4741 catcctctgc agatataaaa gagaaaaaaa cgcgtgacaa agtcattgct ttagcccggc
     4801 gccatggctt aacgcccctg gatgaaatta tccccgaatt cgaaaaaagc cactaccacg
     4861 atgacaagct cttcgttgtt gacacagccg ggaactataa agacaaactg ccaagcataa
     4921 gagacagatt tgcattcgga aaaatacagg attcagaaac atatttcggc gctaaaggcc
     4981 catatactaa agtcacagaa aaaatgattt atgcgcgcag ccccgatatt cacgaataaa
     5041 catgaaacca acagcgaact cctgccttga ttcttcacat gaaaacggga attatgcctg
     5101 catttaaagc ataccaagaa aaaacttaag acacatgaac acaccgacac ctgaaaaacc
     5161 cgccacttcc gaacgcgaag aaactctggc tgagcgctcg gcccgtctcg aatccgaagc
     5221 agccggcctg tataacaagg acgaacaaga cccgctcgcc ccggaagcca tcgcagaaac
     5281 agacgaagaa aggcaagacg ctccggcaac cgcttccggc gtagagctag tagaactaaa
     5341 agcgctgcaa gaagaactcg ccgccacgcg cgaccaggcc aaacgcgctc tggccgaagc
     5401 cgaaaacacc cgccgccgcg ctatcaaaga acgcgaagac gcttcaaaat tcgcagtctc
     5461 cggattcgca aaagatttac tggacgtttc agacaacctg cgccgcgctc tggaagccgt
     5521 gcccaccgat ctgctggaaa gcgcgccgcg cctcaaaaac cttgtcgatg gcatcgaagc
     5581 cacggagcgc accctcctgc gcaccttcga aaaacacggc atcgaaaaaa tcgaaccgct
     5641 cgaccagccc ttcgacccga attatcacga agtgatgttt gaagccccgg ttcccggcaa
     5701 accggcaggc accgttatgc aagttatgga atccggatat gtcttgaatg gccgtatcct
     5761 gcgcccggcg cgcgtcggcg tcgttaagga tgatggcacc accccgccca caggcgaaac
     5821 accccccggc ggccatattg atacgcaggc ttaaaaaaca tcggtagaac caaaggtcac
     5881 aaaatgtcta aacttctttc ttgggccttt ttaacaacat gcgttttttt tacattactc
     5941 cttgggtttt tatatctgct ctacatggaa agaagcatca gtccgtatgc cccccaaata
     6001 tccttcctta acttcctgtc aggagtaaat gttcttccga aaacactgag caccgtacac
     6061 cctgttattc aaatcacaag tctcacaagt ctttttttat ttgcccaact ggcatatgca
     6121 ctactcatac aaaaaacact tatatgctcg aaacaagaaa ccaatatttt tcaaagttac
     6181 aaatgggcat acaaaacaat tttctcaaca aaatggtttg ttgtaagcat aagcggcata
     6241 ttgtgcttct tacttacagc cccgaatttt tattattcat acaatatact acaagtagca
     6301 acgttcctcg ctacaatctg gcttgtggca atttttcctc taagcgcatt caatcaagac
     6361 ataatacact caaatcaacc caaaaactgg ttaaaaccag tatggccagg atttgaaaca
     6421 tattctttat ttctgctgtt ttcctgtttt tatgtactgg caatagttaa agaaatcacc
     6481 cccgaaaatc ttatactcac agcattagca atagttttca gcatatcagg aacatttata
     6541 ttcacttact tatcttttca aattctgcac aaccgatcag tatataaaaa cgttttcaaa
     6601 accattaaaa gaacactgcc ttccagaatt tacattggct ggctcgcgct tttcatctta
     6661 ctgccgttct tcgccgctat ccccttcctc ataatattat ttttcttatc atggggatac
     6721 agtgacatat tgccaaaact tgtaagcaga tataaaaaca ctgacataaa cctttcagag
     6781 acctttaaaa gctttcttaa ttggcttgat aatttttctt atggatacgc cgaaagcact
     6841 tatatgctga tttgcactct actattgacg tttctaacac tatctactgg cagatatttt
     6901 tacttgtctt tacaaaacaa cacaaagaaa gaaagctaaa aatcacgcct gtagaacttg
     6961 caattttaaa caacgctctt atattactcc ttaagaagtg ctgtgaaaaa cgggctttga
     7021 agtatataaa tcttgcttaa gttaaggaga ttaaaatgag caaaattatc ggtatcgacc
     7081 ttggtacaac caattcctgc gtcgctgtta tggacggcaa ggaaacccga atcatcgaaa
     7141 acgccgaagg cgcgcgcacc accccatcca tgatcgcctt ttccaaagac ggtgaacgcc
     7201 tcgtcggcca gcctgccaag cgtcaggccg tcaccaatcc cgaaaacacg ctttacgcga
     7261 ttaaacgcct gattggccgc ccttttaaag acaaacaggt ccaggacatg gcctcaaaag
     7321 cccccttcaa aattatcgaa ggcgaaaacg gcgatgcctg ggttgaagta aacggcgaaa
     7381 aatacgcccc atcacaaatc tcggcgatgc tcctgcaaaa aatgaaagaa accgcggaaa
     7441 acttcctcgg cgaaaaagtc acaaaggccg tgatcacggt tcccgcatac ttcaacgact
     7501 cccagcgcca ggccaccaaa gacgccggta aaatcgccgg tctggaggtc gagcgtatta
     7561 tcaacgaacc cacagccgcc gcgcttgcct atggtctcga taaaaaagaa cacggcacga
     7621 ttgttgtcta cgaccttggc ggcgggactt tcgacgtctc cgtcctcgaa atcggcgatg
     7681 gtgtttttga agttaaatcc accaacggcg acacattcct tggcggtgaa gactttgact
     7741 tgcgcattat cgattacctc gccgatgagt tcaaaaaatc cgactccatt gatttgcgta
     7801 aagacaagct agccctgcaa cgcctgaaag aagcagcgga aaaggccaag attgaactct
     7861 cttccaccac gcaaaccgag gtcaacctgc cattcgtcac ggctgatgcc aacggaccaa
     7921 agcacctgca agtgtcctta agccgagcca aactcgaaag tctggttgaa gacctcatca
     7981 agcgctccat cgatccggtg aaaaaggcgc tcaaagatgc aggcctaaaa cccgccgata
     8041 ttgacgacat aatcttggtc ggcggaatga cccgcatgcc gaaagtcatc gaaacggtca
     8101 aaaactattt tggcaaagat ccgcacaaag gtgttaatcc tgatgaagtc gtcgccgacg
     8161 gcgcagctat tcagggcggc gtactgcaag gcgacgttaa agacgtgctg cttcttgatg
     8221 ttaccccgct ctcactcggc attgaaacct taggcggcgt cttcacccgc ctgatcgaac
     8281 gcaacacaac catccctacc aaaaaatcgc aagtcttctc caccgcagag gacagccaaa
     8341 acgcagtaac aatccgcgtc ttccagggcg agcgtgaaat ggccgctgac aataaaattc
     8401 tcggccagtt tgacctcgtg ggcattcccc ctgctccgcg cggtatccca caaatcgaag
     8461 tcactttcga catcgatgcc aacgggatcg tcaatgtctc tgcaaaagac aaggctacaa
     8521 acaaggaaca gcaaatccgc attcaagcct ctggcggctt atcagacgcc gatattgatc
     8581 aaatggtcaa agacgcagaa agccatgccg atgaggataa aaagaaacgc gaaactgttg
     8641 aagccaaaaa tgtaggcgaa agcatgattc atgcgaccga aaagtcgctg gaagaacttg
     8701 gcggagattt gccgaacgac gaaaaaaaca aagttgaaca agcaattcag gatctcaaag
     8761 acgtactcga aagcggaaat gccgaagaca tcaaagccaa aacggaaagc ctgtctcaag
     8821 ccagcatgaa actgggggaa ctggcctacc gcaaggccca ggaagaggca gctggtgaag
     8881 cagaaacgca agaagccgaa cctgtagacg ccgatgaaga cgaagtcatt gaagccgatt
     8941 tcaccgatct tgagatcgaa gatgaagacg gcaaagatca aaagtctgct taaattaatc
     9001 cattacgtca ttgcgagcgc aagcgaagca atctagtatc tggattgccg cgtcgggccg
     9061 aaaagccctc ctcgcaatga cgattgttaa aaactgggaa tacaggcgtg aaaaacaata
     9121 catcatatta cgagatcctc aacatctctc cgaaagcttc ggacgatgaa atccgccgtg
     9181 cctatcatac actggccaaa aagttccacc cggatcgaaa cccgcaaaat cgaagactgg
     9241 ccgaactccg cttccgcgtt atcagcgaag cctatgccaa tctcaaaacc cgggaaaaac
     9301 gcgccgccta taatcgcacc cgccgtctac aggcccaaaa cgacaacaaa aacactggca
     9361 attttttctc tcaaatcagt gagattttta agcccaaaaa caaagagatt aaaaattaat
     9421 gcccaatgat ttatacaaaa ccctcggagt cgataaaacc gccagcgccg atgagcttaa
     9481 aaaagcctat cgcaaactgg ctatgaaata tcacccagac caaaataaag acaacgccga
     9541 ggctgaagct aaattcaaag aagtcagcca cgcctatgat attctcaaag acgaacaaaa
     9601 acgcgccgct tacgaccgtt atggcgaagc cgcattcgac ggttctatgg gtaccggcgg
     9661 tgctggcggc cctagcggat tcggcgcaaa cgcaggcgcc ttttccgaca tcttcgaaga
     9721 catgttcggc gaattcatgg gcggacgcgc aggcgcaggc cgcagcggca aggcccgcgg
     9781 ctccgacatc caatacacgt tagaactcac acttgaagac gcctacaagg gcaaagacgc
     9841 gcaaatcaaa atccccgtca atgatacctg cgaagcgtgc aaaggctctg gcgcacagag
     9901 cggcacaagc gccggggatt gctcaacctg cgatggccag ggccgcgttc gccaacaaca
     9961 gggattcttc accatcgaac gcacctgccc gacctgccac ggcgctggaa agatcatcac
    10021 cgacccgtgc aaaaagtgcc acggtcaggg ccgcatcaaa aaagacaaaa cgctcaaagt
    10081 caaaatcccg cccggcgttg ataccggccg gcgcatccgc ctgtccggcg aaggtgaagc
    10141 cggtgtccaa ggcggcccgc gcggcgatct ttatgtcttg ctggcgatca aaccgcacaa
    10201 actcttcaag cgcgatggcg caaacctgtt ctgccgcgtg cccatcaccg ttacaaaggc
    10261 tgcgctaggg ggtgatctag aagtccccac catcgaaggc aaggccacaa aagtcaaaat
    10321 cccgcccggc acccagaccg gccagcaatt ccgcctcaaa ggcaaaggta tgcccattct
    10381 gggaagcgac tcaaaaggcg atctttacat cgaaaccttt gtcgaaaccc cggtcaacct
    10441 gaacaaaaaa caacaagaca tccttaaaca actcgacaag gatttcagcg aaaataacgg
    10501 cggaggcaaa cactcccccg aagccagcgg ctttttcacc aaagtccgtg aattctggga
    10561 tgacttgaag gattaggctt aataattccc atggccctgg acacccttat aaccccgcta
    10621 aatttcaacg atgccatcat tggcaaatcg atcaatgaca aaaaaagcgg cctcgacatt
    10681 atcgtgggct atatcagcgg catgcctcca gatctggcag aaatggtcga acaatttgat
    10741 tccatcggcc tcgccttgat gaaccgcggt atcggtcagc atgaacttga ccaggcctgc
    10801 caaaaactgg ccgaacaatg ccaaaatgaa atttcttctc tgctcataac caaaagcgac
    10861 ctcccctttg atgccagatg gtatctcatc ggcgatcttc tccagatgct cgaacttgcg
    10921 cgaaaagaca taatttcttt gccttttagc gatttcctgt atgatgagct ttcagatttt
    10981 ttaagaacat atagatgata agtaattata attaatatat ttttcagaca gaacgcaaag
    11041 gaacgacaaa atgaaaatag gtgttacagg atgcacgggc cgagtcggaa gcctgatcac
    11101 gaaagaactc ctttccggag actggaaaga attagagctg gctggcggca cagtgctcaa
    11161 cgaagatgaa atcaccgatg acttcttcat gaccaccgac gcagaagacc tcttcgcccg
    11221 cgccgatacg atcatcgact tcacatcgcc aagggccgtc ccgacccaca ccgaactggc
    11281 cgccaaacac ggcaccattc tgattatcgg caccagcggc ctgagcgaag ctgacgaagc
    11341 cctcgttaaa caggccgcca caaaaacccc cgttgtctat gccgccaata tgagcgtcgg
    11401 cgtcaatcta ttgatcgcct tggtcgaaca ggccgcccgg cgtctcgatg atagctggga
    11461 tgcggaaatc ctcgacttgc atcataaata taaagtcgat gccccttccg gcacatctta
    11521 cgccttagcc aaagccatcc aaaaaggacg cggtaaagcg ttcgacaaac aagatctcac
    11581 attagcgcgt gaaggccata ccggtgcgcg cgaagctgga aagatcggct tttccgttca
    11641 gcgcggcggc gatgtcgttg ctgaaaattc caccattttc tttggcatgg gggaacgcct
    11701 cgaaatcacg cacaaagcca cagaccgcgc cattttcgca cgcggcgctc tgcgagccgc
    11761 actctgggcc aagggccgcc ccgccggcct gtattccatg aatgatgttt tggggctata
    11821 gagtcaaaac ttatagcttc tccatccgaa gaatcttgcg ctttaaatcc gcgccccagc
    11881 cataattccc cagcgcaccg gatttttgta ccacgcggtg acacggcacg attaaagaca
    11941 cggggttttc ccccaccgcg cttccaacgg cgcgcgccgc cgcagggcgt ccaatatcat
    12001 tcgccacatc accataacaa agcttttgcc ctttacgaat cttcaaaagc gccttccaca
    12061 ctgccttttg aaacacgctg ccatgcaaat caagcgcaat ttcattctca cgcccctgcg
    12121 cccaggcctc catcacctga tcccccagat cgcgcagctc atcatcatca tgaacaaatt
    12181 ctgcgccttt aaaatgcgcc cgcatccgct ccagcccatc gcccttatat ccggcgacca
    12241 taaaaccgag ccagcacaat cccttctccg tctttccaag caccatttcc ccaaggctgg
    12301 atggataaaa tccgtaaata attttttcca tgacgaatcc ccttttgctt gttatgtgtt
    12361 gaccatgact ctatcgcaaa aaaatatcca actttttttc tcccgcctgc gcaaaattca
    12421 gcctgagcca aaaagcgatc ttaaatggac caacgcctac accttgctgg tcgccgttac
    12481 gctttcagcc caggctaccg atgccggcgt caacaaagcc acgcatgaat tgttcaaaat
    12541 cgccgacacg ccgcaaaaaa tgttgggact cggaatcgac ggccttaaag agcacatcaa
    12601 aaccatcggc ctgtataaca acaaagccaa aaacatcatc gccatggctg aaattctggt
    12661 caatgaatat gacgggcaag tgcccgacag cagagataaa ctcatgaagc ttcccggcgt
    12721 aggccgcaaa accgccaatg tcgtactcaa tattgttttt aaacaaccga ccatggccgt
    12781 cgacacgcat attttccgcg tcgccaatcg caccggcatg gctccgggca aaacacctga
    12841 agccgtcgaa caagccttgc tgaaagccgt gcccgatgaa ttcgccctcc acgcccatca
    12901 ctggctcatc ctgcacggac gctatacctg caaagcgcgc aagcccgatt gtccaagctg
    12961 cattgtcgca gatctatgcg aatttaaaga aaaaacacat ccggactaaa aactataaat
    13021 tttatagaaa actgtaagat cagttctcgg tttcatgggg tgcaatcagc accgtctcat
    13081 cgaaaagcaa tatgtcgtta aacaaacatt gcgcaccgac cttagggata taatattgcc
    13141 gagcccagcc ttgttgcgct tcaaattctt ttcggcattt atcggcgcac tgcgtttcag
    13201 acatcgcaaa caatatccgc agacccgatc catagctgtc ttcattttcc ggatccatat
    13261 agcttttaag ctcaaccgta caatgattgc gataaatttg cgtacacgca ggcggattca
    13321 gacatgtttc gtgctgccag gcaccgcatt tcccttgcac gcactttaac atgatctctc
    13381 cggcatattt ctcgctctgc tccccggggg ccgacaccgc acaatccatc gctctgggat
    13441 caagcccacg cttattataa taataatcct cggcagcctt atagtggtcc tgcgatacgg
    13501 caagcaggga attacagccg atatacatca gtcggcagtc ctgatccgca tcacatgccc
    13561 gccatcgccc cttgggcggc cagcctgcaa atacatcgag atcaatctgc cccacaagag
    13621 aatcgtcccg atgagcaaag aatgaatcga cgaaggcgat attctctgga ttttccatcc
    13681 cattttcata ttgaaggatt ctagagccag tcataaccgt atcgccaaga taaagccatt
    13741 tctgtccggg agaaaaacga acatgacagg aactgctgcc cggctgaacc gtaaaaacat
    13801 ttccggctga tgcgttttta tagccctttt caatattgaa aacgacctcg ctttctttga
    13861 ggctttgcac cactcctata aaaacaacgt ctgcctgatt ataaagcgtc tcaaaatcct
    13921 gtggtatata acggcatgcc cgtgcgggcg tataagcaat taaaaacagg aaaagtaata
    13981 acaaaaattt catgctggca gttttatcaa cttttcatgt tttttcatag agttaatctc
    14041 aaacccttag ttcgacttat aaaatccatc cagccgcacg aaacgcgcct ccgcatttgc
    14101 acgaaccaga ttctcaagac actcctcctg aacgtcctga tcgctcatgc ctttttggcg
    14161 cgcccgcact tcataatgcg ccacaggagc ctctgacacc tttttcacac cgtcagcaac
    14221 cgtaaaatat acatccaaaa cgcacacctt attacgatat tgccaaatca ccgttggcaa
    14281 atcccggcgc accaactcag gctggctcaa aactacacgc acatcgcgag cgctaagaga
    14341 caaaaggctt tcactccccc ccaacatcac ccggcgaaga gcccgcttat cggcgcgcga
    14401 atactcggca tcacgcacaa tcatcttacc tgccaagccg cttgctgcct gtgcctgtcc
    14461 gtaatcttgt ttcaaactat gaatagcaaa gaaaaatcca acaataacaa acaatactat
    14521 cagtaaattc agcttacttt ggtacataaa taaccgctct cttgtgattt tttatttgcg
    14581 ccatcaaagc gctatcggca tttttggata tatggcccca tcaggcccgg ttacaaaaac
    14641 gtaaaaacgc ccgcctcaaa aatgcgccct tcccgcatag ctacatacaa gaagccaggc
    14701 ccttataaag cctggcttcc aaatgttcaa caaaaatatc aagtgtagca gtataaaacc
    14761 acctcaaaac gcctattcag cagcggcagc agccttactg aacaaacctt catcattgtt
    14821 agcaggctca ggttgccgct caactttctc tactttgggc actgccgact tcgtgacctt
    14881 tggagctgcc gatggctttt ttgccgccga cggagccgct gacaaaggcg taagcttacc
    14941 atcaataatt gctcccgctt ccagagctat ctctttataa gagatagacc cagttactgt
    15001 tcccgttgat gaaacagtta aacggccact cacagtgata tcaccttcaa aacgacctgc
    15061 gatattggct tcgttgattt caacagtgcc atagaatgtc ccgctctctg caatttccag
    15121 aacattcgcg cctttaagcg cagcttcaac cgtcccttcc accagcaaat aatcacaaga
    15181 ctcgatttca ccagacattg tgataccaga cccgattgtc aaccgacgat cagaaccggt
    15241 atccgcagga ctgccgccca aaccagggta agcaccggga taaccgcccg ctgcgcgaac
    15301 cggagcctgc ccggggcgtt gatatgctcc catcactgct ggcgcactgc cttcttgttc
    15361 tgttgctgtt ttatcttgat ctgtcatggt cccttgatcc tcttcttggg gtgtttctac
    15421 tgtgcttgtt gatgactttg gctcagcctt ctcatctgag tcaggccctt taaactcggt
    15481 aatatcgtcc cgcgtctgtc tctgagcatc ctgctcgtct gtggtaggag cggaaacctt
    15541 ttccggctct tggtcttctg atttaacacg atgaaacata tggcacgtac ctctcgataa
    15601 aaaaatttcg cttgagcact ctctccagat ttcaagcggt ggtttaacga ttactgagta
    15661 tagaccgaac gctagcatgc gatcaaaaac accgcaagac ccgttttaag aactttctta
    15721 taatccactg aattcaataa atattttttt gtgaactaag attctatctt aacaaaaaat
    15781 taacctaaat tatgaataat ggtgggacga taatagggga agtgtaaaac acgatggcaa
    15841 agtcaggttt cagtttttta agcttgctgg gcattaccga ttcaccggaa agctcaccgg
    15901 aaagcttgcc taaaaacgca gccaacaatc acgtttacga cgttcagatt gtccctggtg
    15961 aaagtaaagc cgaaagcacc gcaaaaatta ccgtcgacaa aggcgccgtt gaacagattg
    16021 aaggcgacac acttgaagac aaagctcaag ccattgaaaa agaactcaat gaggcgaacc
    16081 agcaatacga catcgccggt ccagcagaat atgacgttgc catcgtctgc gacgacacat
    16141 cccctcaatc aaccgcaccg gttgcgcctg ctgcacccgt agagatcaaa catttccgcc
    16201 aggaagaatc tgttatgggc attcaaagct atctcatcgg cagaggccat gaacgtgttg
    16261 gagataaaca caataatccc gacggaatgt tcggcaacgg ctcaaacaaa gctctggccg
    16321 aagaaatcaa aagctttcaa caacaaaacg gcatagagcc aaccggcgaa tacaatgacg
    16381 caacccgtca agcctatctg acacaagctg caaaaatgga aggcggctcg gccgggcgcg
    16441 atgcccttga agggttcgtc gaagggcttg atgctatgaa aaataaagac attgatggtc
    16501 acagtgccct tgatcgtgtt taccacccgg aacaagctgt agaacaagcc ccacaagcag
    16561 gtatgtgtaa aatcacaatc gatacctcca tactggatag cacgtttagc gtcaaaccca
    16621 cttagaaata accagcacgc tccactcaat gaaaatagtc ataaataacc tgtgcaagag
    16681 attctgaaat accttcaacc ttttgcagat cttccacccc cgcgccagcc accgccttag
    16741 ccgatccaaa ataattgagc aacgccttct tgcgctttcc ccccacgcca ggcaccccgt
    16801 caagcggcga agcgttaatc tgtttttgcc gccgcgcccg gtgcgtccca atagcaaacc
    16861 ggtgcgcctc atcacgcaaa cgctgcaaat aatgcaaaac cggatcattc accggcaatt
    16921 gaaaactcgc ccgtccaacc atatgaaact gttcacggcc cgcattacga tccggtccct
    16981 ttgaaatcgc caccagcgtg agcgcatcaa gtacgccgta atcctccaac acttcggcac
    17041 aagcgctaag ctgcccttta ccgccatcaa tcaataataa atccggccaa ttcggtccgg
    17101 acatttccgg cgcctctttc agcgcacgct taaagcgccg ctccagaact tcgcgcatca
    17161 tgccgtaatc atcgccggct tcagcccgtt taatattaaa tttcctatac gcccccgtat
    17221 taaacccgtc cggcccggcc acaatcatac cgccgaccat attcgtcccc gaaatatgcg
    17281 agttatcata aacttcgatc cgcttgggaa gctcatccat cccgaacaga tccgccaccc
    17341 cggcgagcaa cttcgcctca ccggcttttt caaccagatt acgggccagc gcctcacgcg
    17401 cattgcgcag ggcaaaatcg acaatccgct tacgcccgcc tcgctggggc tttgaaatca
    17461 caaccttatg cgaaaccttt gagctaaacg cctcctcaag caacgccttc tcttccggca
    17521 atacattaac cagcacattt ttaggaacca gcttattctc atagaactgc gccataaacg
    17581 ccgccatcac attcgcaggc tcctcatccc ggtcatgacg gggaaaataa gaccagttac
    17641 cgtaattgcg cccgccgcga aagaaaaaca cctgcacaca agaccgcccc tctttctgcg
    17701 ccagcgccat cacatcgaca ttccccagcc cggcaacatt aatgtcctgc cgcgcatgaa
    17761 tagagctaag cgcctgaatc ctgtcccgat acaccgccgc cgcttcataa ttctgcgcac
    17821 tacaggccgt ctgcattttc tccaccatgc gcgtttgtac ggcctgactt ttaccatcga
    17881 gaaaatcttg cgcttccata atctgccgcg catattcttt cttgctcacc ttccccacac
    17941 acggcgcagt gcagcgctta atatgatact gcaaacatgg ccgcgtgcgc tgtgcaaaat
    18001 aaccatccga acaattacga agcaaaaaca cccgctgcaa aaccccaatc gtccggttga
    18061 catcacccgc actcgcaaac ggcccgtaat attgcccttt gcgctttttt gccccgcgat
    18121 gcttcaccac ctgcgggaaa tcatgatcct ccgtcatcag gatataggga aaactcttat
    18181 catcacgcaa aagcacatta tatttaggat taaacttctt aatcaggttc gattccagca
    18241 ataacgcttc ggcttccgta tgcgtatgaa caaactccat agctctggtg atgctaatca
    18301 tccgctgtaa ccggatcggc aatgtttcag ggcgcgtata attcaccacg cgttttttca
    18361 gggattttgc tttaccgaca taaagaacct ccccgccctc gcaaagcatt cgatacaccc
    18421 ccggcgtatc aggcagcttt ttcacatagc ccttaataat acgtgcgccg agttcaaccg
    18481 gcgaaatttt aactacatca tccatggccg ttaatttaag acctccccct tcaggatgca
    18541 atggcgcggc agcacaacca ccataccaaa ggttacataa catttttccc ttgacatcca
    18601 aaaggaaata tcaactatta gttgcataaa aaaacaaaac aaaaagcaac accataattt
    18661 aaggtgtgac gtgtgttcaa aaaataccaa gggaggattt catgcaacac caccaaatcc
    18721 ggtctactgt agacaatacc gttctgtttg aaggacgctt tgaaaacttc aaagcctgtc
    18781 tcgaacaagc ggttgcagat cgggttgcac ttcactgtgt aaacctgcgt aaccaaaacc
    18841 tgaccaacgc caatctcgac gatgcgctca tgcccggtgc cgatttttcc ggcaccaacc
    18901 tcaccggcgc taatatgtca gaatcctatc ttaagggcgc agacttcaaa agcaccgtgc
    18961 tctataacac ctgcttctac gattcaaacc taagcgcctg caatttcgaa ggtgccgcct
    19021 tcggggccac agatattcac ggcaccatcc tcagccacgc gcaattttca acgctctcat
    19081 gtttctgtct cgacttcgtg cgcgccagag ccatgagcgg ctgcatcttc attaaccccg
    19141 atggccgtat atgcgaaatg tccaagccac ccatcgtcgt acgcggcgct ggaaatgccc
    19201 ctattatttt gctcgacaac aaaatcaaat ctggccacaa catcatcgac cacaaacgac
    19261 tgcgcccgct gatagaaaag ctctctgttc gcaccctgcg aaagcgcatt gccacgtgaa
    19321 ttttgtattt tgtgtattgt tttgcgcttt tggaaattga atggcttgaa aataacgccc
    19381 gcacgcccta tgaatgaacg ctgcatgggc ctgtggcgga atggtagacg ctgcggactt
    19441 aaaatccgtt gggagtaatc ccgtgggggt tcaagtcccc ccaggcccac catctaaact
    19501 taatcgccat caatcatcca caggcacctg aaagctcaaa ctctgcgcac caccgccaca
    19561 gcgctctacc aacgccaaaa cctccttcgt cgccgcgcga atagtcgcct catcagttcc
    19621 gcgcaaaacc acgctcacat tcaacgcccc tccgcgaaag gacgggtaac tcccgatatc
    19681 aaccgtttca taacgcccct gaatttcgcg cagcccttcc gccacaatgc tttctttcag
    19741 atcacaaaac acagtattgg ataaaatcgc cgcgcctggc ttaatctcgg caatcacatg
    19801 ctccagcatc gcgcgcataa tccgcggtac gccggccatc acaaacacat tttcaattgc
    19861 aaaccccggc gcacccgaca cagggttaag caacaaacgc gccccgcgcg gcatattggc
    19921 catgcgcgcg cgtgtctcgt tcaactcatc cggcccataa taatccagca acacatgata
    19981 cgcttccgca tgccgctcaa acacaactcc catcgccttg gctatgctgg ttgccgtaat
    20041 atcgtcatgt gtcggaccaa tcccacccgt cgtaaacacg taatcatatt cgtcgcgcaa
    20101 aacattcacc gcctcaataa tcttttcctc aatatccggc accacgcgca cctcagccaa
    20161 aggcacgcca tgtttagtca tatgctcggc aatccagtta atattcttgt cagacgtgcg
    20221 cccggaaaga atttcattac cgataacaac aagtgaagat ctctgtccca tccagaattt
    20281 ccttggtttt tatactgctc acactattat aggcgttctg ggataaagat atattgaatt
    20341 ttaaagcaga tagaccgatt tatgaacacc gccaatacat acacatcaga aggtatcgaa
    20401 ctccacgccg acaacgccga cttcgaaggc atgcggagag ccggaaaact cgccgctact
    20461 attctcgatg aagtcacacc acttgtcgtg cccggcgtca ctaccgaaga gctcgataaa
    20521 ttctgtcatg aatacgccct caaacataac gccatccccg ccccgctggg ttacggcgaa
    20581 ggaccgggcc gccccccctt ccccaaggcc acctgcacct cggtcaatca tgtcgtatgc
    20641 cacggtatcc cgaacgagaa aaccttacgc gaaggcgaca tcgtcaatat cgacgtcacc
    20701 gtcatcctcg atggctggca cggcgatacc tcgcgtatgt atatggtggg caataaacat
    20761 tcggtcaaag ccaagcggct tgtagacgtc acctatgacg ccatgatgcg cggcatcgag
    20821 gccgttaagc ccggtgcaac cctcggcgat gttggctacg ccatccagtc ttatgccgaa
    20881 agtgagcgct ttggcgttgt caccgatttc tgcggtcacg ggctgggcaa ggtcttccat
    20941 gcgccccctt ccgtcatgca tatcggccag cccggcaaag gcacggtgtt agagcccggc
    21001 atgttcttca cgattgagcc catgatcaac gcgggagatt atcgcaccaa agtcctgccc
    21061 gatggctgga ccgccattac caaagaccgc tcgctatcgg cgcagttcga acattccctc
    21121 gccgtcactg acgacggctt cgaaatcttc accctttctc caaaggggta cacaaagcct
    21181 ccttatgaat aaggatgaga tgaaatgatg tttgagataa tatccgctgt ttggcttata
    21241 acctttgcta taaattacct atatattaca cgacatgatg aagaagcctc tcaaacattt
    21301 gtcttgttta tgcttcctct ttcaggaaaa ccaatccaga taatagtgtc gtttgttgca
    21361 agctcaataa tgaccggatg tgttgcagtc ttattaatta tggtagacca ttattttttg
    21421 gatacacagt tttgagtttt taaattaagc catgaccgcc gcacagaaag cacaaaaacc
    21481 acactatcgc ggccaccgtg atcgcctgcg tgaacgcttc gccaaaaccc ctgacgcact
    21541 ggccgattac gaactgctcg aactcgttct tttcctcgcc atcccgcaaa aagacgtcaa
    21601 accgctggct aaagacctcc tcaaacgctt cggcagcctg tccgaaatca tgcacgcgcc
    21661 catcgaagaa ctcacccgcg tcgaaggcat aaaagacaaa accgcgctgg ccctcaaatc
    21721 catcaccgcg ctatccaacc gcgccgccaa aaacgaactg ataaacaaac ccgtcctcaa
    21781 ttcctggacc cgcctgatgg attattgcca cgccacaatg gcccatgaaa cgcgggagca
    21841 tttccgcatt ctcttcctca ataaaaagaa cgaactcatc gccgatgaaa ttcagggctc
    21901 cggcacggtt gaccacaccc cggcctatcc gcgtgaaatc atgaaacgcg cacttgaact
    21961 cggcgcgacc gcgctcatcc tcatgcataa ccaccccagc ggcgatccca aaccctccca
    22021 agccgatgtc gatatgacca cccagatcat cagcgccggg cagcctttca acatcgttat
    22081 ccacgaccat atcgtcatgt ccaaaaacgg ctattccagt tttaaaaata tgggattact
    22141 ctaaaggcca tgctctccat aatccgtcat tgcgagcgta gcgaagcaat ccagaatctg
    22201 gattgccgcg tcagacctcc ggtcttcctc gcttgcaccc ttcagggtgc aatgacgata
    22261 ctagcatgtc ttatcttatc cgcctgcgcg ccgcagcttc ccacttcgga aaagctggaa
    22321 cagatccgcc tcgccaaaca ggacgctttg gccactgaaa taaccgccag cggcctgaca
    22381 ctgggccaac ccacttacat ccgcgtcttc aaatccgaaa atctcgtcga aacatgggtg
    22441 caagatgaaa caaccggccg ctacgccccg ttcaaaacct accccatctg caattactcc
    22501 ggcctgctcg gccccaaact gatggaaggc gaccgccagg cccccgaagg cttctatacc
    22561 gtcacagccc ggcaaatgaa cccgtagagc caataccacc tgtcctttga catcggctac
    22621 cccaacgcat tcgaccgcgc attggggcac accggctcaa acctgatgat ccatggcgac
    22681 tgcaaatccg acggctgcta tgcgctcacc gatccggcca tcgaagaagt ctacctcctc
    22741 accgaagcct caatccgcga aggccacccc gtgcccgtgc acatcttccc gttccgcatg
    22801 catgcccgga atatggccca gcacaacgcc agtccatggt cccccttctg gcagaacctg
    22861 cgcgaaggct acgacgcctt tgaaatcacc agaattccac cggaaattcg cgttcaaacc
    22921 gaccttaccg gcgcaatgta catattcgaa gaacaaaact ttaaaacagc cttgtattag
    22981 aaagttaatc caaatgagcc taagtgacaa aaatccctca attgatcgct tcttcgatcc
    23041 agttataaac tgcacatctc acaacgaact cagaacatgg cttaaaaaac aaaatgaaga
    23101 ggacatcaga acttttttag acacatataa ttttgacacc aaccaggcgg aaaaaagtat
    23161 tttacgacaa gagttagaaa gacgaagctc tattaaaaat caaaaagaca aatggattga
    23221 cagaattatc ggatttgtat ttggaatatt atctggattt ttaatcaact cattttaaat
    23281 gtcattgcga gcgcagcgaa gcaatccgga atccggattg cttcgctgcg ctcgcaatga
    23341 taataaataa taaatgcccc ttggtgcctt ggtgtcttgg tggtaaaact ctctcgcaac
    23401 aaacaattct aaagagacga acaatgatcc cacgctattc ccgccctgaa atggccaata
    23461 ttttcgaaca agaaaacaaa ttcagcatct ggctggaggt agagactctc gcccttgaaa
    23521 aaatggccga tctcggtatc gtccctgaaa gcgcagccat tgccctgcgc ggaaaaggcg
    23581 acttcgacat cgcccgcatc gatgagatcg aggccgaagt caaacacgac gtcatcgcct
    23641 tcctcacctc cgtcgccgaa catgtcggcg aggaatcccg cttcgtccat caaggcatga
    23701 ccagctccga cgtgatcgac acctctttcg ccgtcatgct gacccaggcc gccgatctga
    23761 ttatcgagga tctcgacaaa ctcctcgctg cgctcaagaa acaggctctg gcccataaag
    23821 acacgctgtg cattggccgc tcccacggta ttcagggcga gcccacaacc tttggcctga
    23881 aaatggccgg ccactacgcc gccttcgccc gcgcgaaaaa gcgcatgatc gccgcgaaag
    23941 aagaaatctc aaccattaca atctcaggcg ctgtaggcac ctaccccgtc ctgccgccag
    24001 aagtcgaaca atatgtggcc gataaactcg gcctcaagcc cgaaaccgtc gccacacaag
    24061 tcatcccccg cgaccgccac gccatgttct ttgccacgct cggcgtcatc gccagctcaa
    24121 tcgaaaacct cgccgtcgag atccgccacc tccagcgcac cgaagtccgc gaggcggaag
    24181 aatatttcga ccctggtcag aaaggctcct ctgccatgcc gcataagcga aatcccatcg
    24241 gcagcgaaaa catgaccggg caagcccgca tcatccgcgc cgccgtcatt ccggccctcg
    24301 aaaatgtcgc cctgtggcat gaacgcgaca tctcacactc ctccgttgag cgcacgatat
    24361 tgcccgatgc cctcatctcg gctgactacg caatcaatcg cctcgccgga attatcgacc
    24421 gcctgctcgt ttacccagag cgcatgatac acaatctgga aatgaccggc ggactgctct
    24481 tctctcaacg cgtcatgctc gccctcaccc aggaaggtat ctcccgcgag gacgcctacc
    24541 gcatcgttca gaaaaacgcc atggccgttt gggaagaccg cgccaacggc aaaggctcca
    24601 aaaccttacg cggcgaactg gaaaacgacg cggctgtcac ggataaactt gactccgccg
    24661 cctttgaggc tatcttcaac tacgatgctt acaccaaaaa tatcgaaagc atcatcaacc
    24721 gcgcacttgc ttaaggagac ctgccatgaa aggtgacaaa aaagtaatcc aatacctcaa
    24781 tgatgccctc aaaaaagagc tcggcgcgat caaccagtat ttcctgcatt cgcgcatact
    24841 cgaagattgg ggcatcacca aactcgccaa acacgaatac aacgaatcga tcgaggaaat
    24901 gcaccacgct gatgaactca tcaaacgcat tctcttcctt ggcggcctgc caaacctgca
    24961 ggaagtcgcc aagctttata tcggcgaaac cgtcaaggaa acccttgaat gcgacctcaa
    25021 aattgaactc gaaggcgtaa ccggttacaa agaaggcatt aaatactgcc gtgaagtcga
    25081 cgacttcgta accgaagatc tgttcaaaaa aatcctcgca gaagaagaag gccacgtcga
    25141 ttatctcgaa acccagcttg aaatgatcca gcaaatgggc ctcgaaaact acattcagat
    25201 caactcccaa agtccgctcg acaccgaagg agaataggac tttgaaggaa tcatccctcc
    25261 ttcagcttcg cttaagcttg aaatgagaaa ataaagctat tgagaatgat tttcattact
    25321 gatattgtga cgataatgat tcttaataac agcttttaaa cccaaggatt gagtgtccac
    25381 aatgttcgtg tgtctatgtc accccttcaa cgataaaaag gttaaagacc atctcgacgg
    25441 ccacggaaaa cgtgcccgtg tcggtgacac ctaccgcgcc tgctccggcg gcgaaaaccc
    25501 cgaatgctgc caatgtctct ccaccctcaa agacatcgtc aaagatcaca acaaaactgt
    25561 gacagcttaa acacaccaac tcgcgctaca aacgcattct ctcttgcatt ccccacaccc
    25621 ctatggcacc actttagaac acattttttt aaacaatatt aggggaatac cacatgttta
    25681 agaaactcat tctgactgcc gggagcttgc tactcatgac cacaacatca aacgcggcca
    25741 gtgacgccga tactctgaaa ctcgaactat caaccggagg aacggtcagc atcgaaatgc
    25801 tgcccgaccg tgcgcctgca catgttgagc gcatcaaaaa actcacccgc gaaggctttt
    25861 acgacggcat cgtctttcac cgcgttatcg acggcttcat ggcccaaacc ggcgacccaa
    25921 ccggcaccgg aacaggcggt tcaagcgagc ctgacctgcc tgccgaattc accgactacg
    25981 catacaaacg cggcacaatc ggcatggccc gcacagccaa cccaaacagc gcgaattctc
    26041 agtttttcat atgcttttcc gatgatggct gctccttcct gaccggccaa tacacggtat
    26101 ggggccaggt tacaggcggt atggaatttg ttgatgcgat tacccgcggt gagccgcctg
    26161 ccaaccctga caaaatcatc aaagcgatga ttgccagcga cgcgacaaat aattcagccg
    26221 cagcagagac acctgctgcg ccagaaacca aagcgcctgc cgaagctgaa atcacagaac
    26281 ctgccgctgg tgaaccttct gcagaagaat ccgacaacag cgaagccgct gctgagtaaa
    26341 cggctaaaat gaaaacgatt ggaatacttg gagggatgag ctgggtatca tccctccatt
    26401 actacaaatg gctcaatgaa ctggcgcagg accaccttgg tccgatcaat aattccaaaa
    26461 tcctgctcaa ttccgtagac tggcaaaaaa tccaaaaatt tcagaaagac gacgactgga
    26521 atgacgctgg caattacttg gccgccgaag ccagaaaact cgaatccatc ggcgcggaac
    26581 taatgctcat cggcaccaac accatgcaca aagtcgcaga tcagatccag gccgcaatca
    26641 atatcccgct actccacatc gctgacgccg ctgccatgcg catcaaagcg caaggcctaa
    26701 acaccatcgc cctgctcggc actaccttca ccatggaaca agacttttac aaatcccgcc
    26761 tcgaaaattc aggcatcaaa gtccttatcc ccgaaaatca aaacgaccgc gacaaaatca
    26821 acgcgatcat ttttgacgaa ctctgccaga acgttaaaaa aacagaatca aaaaccgaat
    26881 atatccgcat cactcaggaa atgctcgacc aaggcgccca aggcctcatc atgggctgca
    26941 ccgaaatcac caccctgatc ggcgcggacg attttgacaa acccgttttc gacaccaccc
    27001 gcatccatgt cgaagaagct ttcgaactcg cacaggagaa ctaatccatg tctaacaact
    27061 ggtggcgcgg tgccgccatt taccaaatct acccccgttc cttcttcgac accaacggcg
    27121 acggcatcgg cgatcttccc ggcattaccg aaaaactcga ttacatcgcc tcactaggcg
    27181 ttgacggaat ctggatttca ccctttttca cctcaccgat gaaagatttt ggctatgacg
    27241 tatccgatta ccatgacgtc gaccccatgt tcggatcgct cgaagacttc aaaaccatgc
    27301 tcgtcaaagc ccacaagctt aacctcaaag tcatcattga tctcgttctg tcccatacct
    27361 ccgaccaaca cccatggttt aaagaccccc caaaaaaaga ctggtatatc tgggccgacc
    27421 ctcggcccga catgttcggc gaaagagtcc cgcccaataa ctgggtctcc gtcttcggcg
    27481 gcccggcctg gacatatgac gaaaaccgcg gccaatacta cctgcacaac ttccttcccg
    27541 aacaacccga cctgaatttc cacaaccccg atgtccaaaa tgccatgctt gatgtctgca
    27601 aattctggct gtatatcggc gtcgacggat tccgccttga tgtcgtcaat ttctacttcc
    27661 atgaccggga attgcgcgac aacccaccgc gcgacgccga ccttggccca gccacccaat
    27721 acgaaggcga cgatccctac tctgcccaaa accacatcta cgacaaaact cgccccgaaa
    27781 acccgatatt catacagcgc gtccgcgcac taatagacca ataccctgaa cgcattatca
    27841 tcggtgaagg cggcgacgat cacttcttca aactcgccgc cgattattgc agcggcgaca
    27901 aacgccttca taccatatac agcccccaac tctgcgccgg agcccataag cgcctcaccg
    27961 ccgatcttat ccgcgatccc gttgaaaatt ttctaaacca ggcccccggc gcatggccca
    28021 catgggcctt ttccaaccac gatgttgtcc gcgccgcttc acgctggtgc gataaaggcg
    28081 aaggattcga ccataacccg cgtctgtcca aaaccctcat cgcactactg ggatgcctgc
    28141 aaggctcttt cttcctctat caaggcgagg agctcggcct gccagaagca aagctgaaat
    28201 tcgaagattt acaagatcca tggggcaaac atctctggcc cgactggcaa ggccgtgatg
    28261 gctgccgaac ccccctgccg tggaataaaa tgccgcaagc cggttttaca accggcaagc
    28321 catggctgcc aatggccgaa acacacctgc ccctcaacat tgaaaaacaa gacagcgatc
    28381 caacctccgc actcaatttt acccgcaaat ttctaagctg gcgcaaaaat cagcccgccc
    28441 tcatttccgg caccatcacc tttctcgaaa cagactcaga tactctgctg gcccttgagc
    28501 gtaaaaataa aggtcaaact ctgatctgca tttttaatct ggaaaatgcc agccagacac
    28561 tgccgccatt tcttttaccc gataatatag aaacaacctt cccgaccaaa aaccaatcca
    28621 cagcccccat ggccccgtat gactttcaaa tctggagata cacttaatct ctaggcttag
    28681 ctttcttttg tgaactcttc tgagtcttct caacaggctc agtctcttct ttttgcggct
    28741 gcggctctga ctcttcacca tcgcccgcat caagatcatc atcctcaagc tgcaagccac
    28801 ttgcgcccgc ctccaccgta tcatcaagca ccaaagaaga atctttcccc accccgcgca
    28861 cctcttccgc cttaagcttt ttggcttcat cggaagacac ttccaaggtc ttcgccgcct
    28921 tcaacaatag cttcacaaac ctgtccaaat attctgcaga aatcatagga tcggccatat
    28981 caacgcgcaa taacatccga tcccccttga agattaaaac catacaagag tttttaatct
    29041 tcataagttt gacaagtaat tcaatccgct catctgtaag atatgcatcc aaaacaaacc
    29101 ggttatcccc cacaaccaaa tattccaggc tccaatcttt gtgcttaggg cgaacttcca
    29161 tcttaaaatt caacgttttg atgaaggaaa tcatctgacc actcgcaata gcaccgtcta
    29221 tcggcatagt gctatgcaac ccgacctcaa cagccgtcaa tttacggctt gaacgcatat
    29281 cgggattcaa atgctcactg gtaaaaaaac tgaccttata ggaatcaatg ttcccttcca
    29341 tctccgcccc ctccatcatg gagttaggct taaagcgcaa tttatgcttg gcggcaaagg
    29401 ctttccacgt cttcttctgc tgatacaaaa tcaacaaaga ccaccctaaa aaaccaagca
    29461 aagccgcaga gataaaaaac caaagtacaa accagatcga caagacgaaa actccttgaa
    29521 catatacgca aaaacgctgc gcacatctct ttctacaaaa agtttaatca atcatcgcac
    29581 ccttggccag cgccgtaaaa agatataaac gaaaaaagct attctacaca aagacagcca
    29641 tatatcacga tgacgaaaac aaggaatgtt ccaacgcgtc caaatcccgt gacaatgctt
    29701 tcagcaaatc ttccatcatt tcgcgacgcg ccggatctgt ttcagaaccg caatcaacaa
    29761 aataatgagt cgcacaaagc tgtgctacac aaagacggca ccgttccagc gccaattctt
    29821 gacagaatcg ggggtattcc ataataactc tccacacaca aaaaaaattc aactagagca
    29881 ttctaatatt tcattttaaa aaataatgca agttttcttt caatccctct ttgacttttt
    29941 cgctcatata aatagattac taatataaaa aggatgtaag cacacatgat ctcaatcgaa
    30001 caatgcagag gcgcacgcgg aatcctggga tggactcagc aaaacctcgc tgatgcctct
    30061 ggactttcca aaacggctat caataatttt gaaaaaggtc attccgacat caaggcagaa
    30121 tccctgcgcg cgatccgcat ggccttcgaa agcgccgaaa tggaattcct ggggcaaaac
    30181 ggtattcgca agaaaaccga agatgttcgc atcctccacg gaccggaatc catgagcatc
    30241 ctcctcgaag acatccaaag caccatggct gcgcgaaacg gagaaatcct gatcatgaac
    30301 gctaacaaca acctgtccag ccaggttcca acgcaaaaac tctttgatca cattgatctg
    30361 attaaaacca accatatccg ccaacgcatt ttatgccctg aaggcaccaa aaacatactc
    30421 agcccgaatg atgaatgtcg ctgggttcct gcagacatag gccagcgctg catactcaca
    30481 ttcatataca ctggcaaagt agccttccag ctttgggatc aatctataat catcatcatc
    30541 gacagcaaca tcgctcacga atccgagaaa aaacgcttcg aacatatctg ggcaaacgcc
    30601 atgccctcca cagagcaaaa aagcaacgcc tcaaaacaag tacaaacata aaaatacggg
    30661 tcctacctaa gcagggcccg tattttattt ctcacgcaat atcaataaat cgacacgcga
    30721 attttacgcc ttaatgatag tggacctctt cttgatctgc aaatccactt tgcaatgaaa
    30781 attgttgtaa atttcgtggc atattaagat gtaacgcctg cccggatacc ggtatatttt
    30841 gaatctcctg ctgtatctgg atctttgacg ccaaatcacc aacctgagac aaaagatcct
    30901 cagcatgagc aaccagagct tcagccttac cggtactgat cagatcatgc gcagccgatt
    30961 tctccatttt ctcagccaca atctcttcat gcccgctaac caaatcaacc tttttctcca
    31021 gtttgtgaat agcatcacta aagaaaccgg tctgctgatc aacaacactg acctttttat
    31081 caagcttggc caccgtatcg ctaaacacat tggcctgttc accaacattg ttaaccttgg
    31141 catccaacgc cgtaatcgac tcgctgaatt tactggcctg gccatcaaca acatcaactt
    31201 tttgctccag cttgtgaata gcatcgctga aaaaaccagt ctgtttgctg ataatttcaa
    31261 acccgttccg aagcctttca aacccggccc caagaaccag agacgttcca acggcggcaa
    31321 ccgcggagaa cacagccatc attagtgaga cagccactat agtttcgaca tccattgtca
    31381 tgaatacacc ctttctctta tgttataaaa tatgataacc aagggacttt cttttgcact
    31441 ttacgaagac ctctcagcca tttacagtga tacattgatc acaaaattta agatgccgtg
    31501 aacgcaccca taattattat gttaaatata atcgtttaat ttttggtgag ttttcaatcc
    31561 aataaacatc aaaactattt caaatttgaa caagcccctt ggtttttacg tgcctataaa
    31621 gtttttcaaa acttttaaat acacaaagag aaattcggcg aaaatttgag ttttcgagaa
    31681 ccgaaacaga acatacataa aagtatttga gtatcggaag cgcagaaaac tcaaatttgc
    31741 aaccaattta tcacagtgta tttgaaagtt ttatttttcc tgctgctcac ggaagatctc
    31801 ttccgctttt tgtggctgcg gctccacagg cgcaggaata tcttcaataa tgtccttggt
    31861 ttcctgcaat ggctgcggcg ccggacgtgc agccacaggc tgcaacttca tctctttttt
    31921 gggcaacatc ttcgccaaaa cataagcatg cccactccca gctgtacgct gattgccata
    31981 tggaaacata gtgtcggttt gaaccagttc aaatccctcc ccgcaagcgc caagcaaaaa
    32041 tggagaaacc aaaagaagtg cacgagccgt tttcatcgca aaaaatcctg aaatatcaat
    32101 gttaaaacaa ggaaagcagt gaggatgcat gaacaccatc caagccctac tatagcgcga
    32161 tcaacataaa atcacaatgg cttataaaat ataagatttt tacaaacacc cacagatata
    32221 ctcacagcct tattcagatg attatgatgc gcggtacaaa accagatgcc ccatcggatg
    32281 ccctttgcgc aaagtcggat acgcaaaatc cccatcctcg tcgcgcacca tcccgatttt
    32341 ctccatcaac tgaatagagc gtgtattgtc atacaccgta aacccgacaa tctctttaag
    32401 cttcaaatcc gtgaggccat aatccaaaac agcgctcgcc gcctcactgc catagccctt
    32461 tccccaaaac tcataatcca gacgccatgc aatttccgtc gcaggcttct tcggcgatgt
    32521 aaaatgcgcg gtaaaacgca cagtatcaag cccgacaaac ccggcgaact gcccgctttc
    32581 tttgacctca acggcaaata acccatatcc tttccgcgca aggtgatctt caaaatgccc
    32641 aaccagctta ttactggctt tttcatccag tggccgcggc atatattcca tcaccagcgg
    32701 atcggcattc agccgcgcaa acggcgcacg gtcttcctct ttccaatcac gcaaaagcag
    32761 acgatctgtc tcaatataaa tattcatggc gcaaaactat ccctataatc ccttccaaac
    32821 aagcccgaac ttttctcctc cccataaaaa catcatacaa tttgtatgac ttgtggaaat
    32881 agactgacaa aacattgccc cgaacctgca aaaatgccag aatagcgcag ggaggacata
    32941 atgaaatttc ttttagcgct ctcatgcgca gccacgcttt cattaactgc catacacagc
    33001 ttcgcaaatg acatgccgct gcccgagcgc aaaccttcat ataccaacgt caacgctgac
    33061 cacaaaacct taagcaaagc aaaatttcta cttgaacaaa ttagcattga gctcaaaaaa
    33121 ccggttgaaa aagtaagcgc gaaaccaacg ccagagccgc cacccacaac aacacccccg
    33181 caagacacac agccatatga actaacagaa aacctccaaa tcagccgtca aggcgacacc
    33241 aatatgcatt gtggcgccct atccagtgaa gccatgcgca tgcgcgatgt aatctacgcc
    33301 acacaggaaa tcaaagccaa atccagaata caaagtcacg cggtaacagc cgcaggcgca
    33361 ataggatcct tgctcatcgg ctcggtcacc ggcggcatcg ggctagctgt tggcggcttt
    33421 ttactagacc ataacgtcgg acacaacgcc aacgaagccg ataacattca ggacatcgca
    33481 gagcagcgcc gcaccttgat gatggggata tacaacgcca aaggctgcga aggcccgcta
    33541 gaccatgcca tgcaaaaccc ggaaattttc gatccactcg cacgtatcgc atcgatagaa
    33601 tccgccgccg gtgatataga tggccacgac ttcaacgatt aatccatatc cttagattga
    33661 aatctctaca aaaattcgct ataaaaacag cctatcgcaa tatcttgagg aaaatcatca
    33721 tgaaacgcac cgccatagcc ctgtcttatt tagctgcatt gacacttcca accgcctctc
    33781 aggcacaaag ctttctcgac cggatagaca acgccgtttc aaaagtggaa aacacaatcg
    33841 cccgcaccga aagaacaatc acacgcaccg aaaacactgc cgagcgcctc aatgacaaaa
    33901 tccccgaagc cggcgcagaa acacctgcag aagaaatacc cgcccaagaa caaacaagtg
    33961 caaataatgg cgtaacagcc gaagatcagc gtattctcga tcaagccaag aaaatcgaag
    34021 aagaacgcgt tttgcgcgaa gctgaaaaaa tcagaacgcg ccgcacaaac acaaacgggc
    34081 gctaatcaga tcaatgaaca cccccgcccc aaggggcgag gtatcatgtc attgcgaacc
    34141 gccaaagatg gtgaagcaat ccagaattta tccttgtggc actggatcgc ttcggtgcgc
    34201 tcgcgatgac gattacgtc
//
LOCUS       DAOSWR010000006        27234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1121_length_27234_cov_5.361823, whole genome shotgun sequence.
ACCESSION   DAOSWR010000006 DAOSWR010000000
VERSION     DAOSWR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 27234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27234
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_27234_cov_5.361823"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK513_01010"
     CDS             <1..816
                     /locus_tag="PK513_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO81067.1"
                     /translation="TLFGLKLLFRKTLSVTPDKPEFESEDMITDKKTWFSSLAVLGVM
                     VVLFVMHGALHWEAWVVACLGLTVLLALTYKANPDKYLTEVELSLLMFFVSLFVIVGG
                     VEGSQFLEWIGQFITPIVREDMLLACLVLMWVAAILSAMIDNIPFTAAMIPIIMGMEA
                     QGIPVTPLWWALAIGVGMGGNGSHLGSTANVFIVTLSERLAQQENKPSLRITPGVWFR
                     KGTPVMLLTLCVCSLAMWLFFPFFAHPLPTAEMHAAKMHEKAAHAMEKTEHHG"
     gene            966..2435
                     /gene="nhaD"
                     /locus_tag="PK513_01015"
     CDS             966..2435
                     /gene="nhaD"
                     /locus_tag="PK513_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017052774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOO81068.1"
                     /translation="MLKRAGYLSLLIVATLFAVCLPTASIWAASNSNASAQTLNAVTH
                     PAAILCLVIFVLSYVAVLMEEYTHMRKSKPVLLGAGLIWVIIGMIAPSYGIDHDTLHS
                     AIFHGLEEYASLLLFLLAAMTYISALEERNVFAVLKSNLIRSGLNFRQLFWATGLIAF
                     FLSPVADNLTTALVLGAVVVAVGSNNPRFVSLACINVVSAANAGGAFSPFGDITTLMV
                     WQSGNVSFFEFFNLFLPSVICFLIPAAIMSVFIPRDYPEALKEDVSLRHGARMIIALG
                     VFTIAMAVGFEQTLGLPPFVGMMFGLSFLMIAAYVIRHVWVNKDEDKDFDILENVAAA
                     EWDTLLFFFGVIFSVGGLAFLGYMELASAAMYQEFGASATNIVLGVSSAIIDNIPVMF
                     AVLSMNPEMDHFQWLLITLTTGVGGSMLSIGSAAGVALMGVARGQYTFMSHLKWTPVI
                     VLGYISAIGVHFLINSHLMNVSIHAIDVTTHIEEPAPTH"
     gene            complement(2432..2953)
                     /locus_tag="PK513_01020"
     CDS             complement(2432..2953)
                     /locus_tag="PK513_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HOO81069.1"
                     /translation="MLETILARIGLPLLITFVSGALKQINTPVTKSAAKALEDVDKAL
                     KAGDISPEAIAESNRHMEALSLMKSEEYREAFKQVNESLRTEIASSDKYIRRMRPTFG
                     YLMAITWAAQMLGVAYIMIFDTQRAGYIMSSMSSLSAIWAVGLSVLGIYVYKRSEDKK
                     LVSPETIFWNASR"
     gene            complement(2957..3448)
                     /locus_tag="PK513_01025"
     CDS             complement(2957..3448)
                     /locus_tag="PK513_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOO81070.1"
                     /translation="MKKPTLTAIGGSDVRPRADAEEFYRALEIDVLARTLWGEARGQG
                     HIGMEAVASVVLNRVKVAQGHGGKYWWGGNIIQVCQKPYQFSCWNRSDPNFQKLQAID
                     DKNLYFASALRIARRAVAGVLPDHTGGATHYHADYTSPYWARDEIPSAKIGHHIFYKL
                     QEA"
     gene            complement(3592..3912)
                     /locus_tag="PK513_01030"
     CDS             complement(3592..3912)
                     /locus_tag="PK513_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81071.1"
                     /translation="MNLLSQDLVWWITIFDLPAMAGLLAFSWRTRREAERAINNLEHM
                     LEHRSDQLRDALSGFKLEVAKTYASQTDLRALESRLVEHLLRIESKLDATALKAEALK
                     AKSE"
     gene            3989..4291
                     /locus_tag="PK513_01035"
     CDS             3989..4291
                     /locus_tag="PK513_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81072.1"
                     /translation="MPNTSVLVPGDKRTKEFLSRAHGYVQVAKDAGLPFNDQLQEAVD
                     FMEIHSQGSSPLSFRVLQASFNRHCGREAQRWINCFKRELVYCTTLPANCTPGDHS"
     gene            complement(4288..5805)
                     /gene="terL"
                     /locus_tag="PK513_01040"
     CDS             complement(4288..5805)
                     /gene="terL"
                     /locus_tag="PK513_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HOO81073.1"
                     /translation="MNEIVKPTSLQSTKFDISEADFPLFVVLWNQRMGLRTPQIHLKM
                     ARWLEWNWTRKNTRLLLMAFRSAGKSTITGLFSAWLLYRKADLRILVLAADFTLAKKM
                     VRNVRRIIERHPLTTKMKPENADQWASDRFTVKRTFEARDPSMMAKGITANITGSRAD
                     IVICDDVEVPNTCDSAEKRRELRERLGEMEYVLVPGGTKIYVGTPHNYHTIYADEPRT
                     QLGEEKEFLNGFERLKIPILDEKGQSAWIERYSEADIAGMKRAAGPNKFASQMMLQPV
                     NIAEGRLNPDLLRHYDEPLDYCKELQTLFLGQTKMVSVSAFWDPAFGAAKGDHSVMAV
                     IFSDAEGNFYLHHIEYIRTDAGNEQDEATQQCRIVSQIAKNHYLPAMTVETNGIGRFL
                     PGILRNELARAKAPCGVQETSQSRAKDLRILEAFDAVLAARRLHIHKSILRTPFMTEM
                     REWRPESNKGHDDGLDAVAGALSQAPDRLPRLYGRGGHNWMKSARTHKADTDFRV"
     gene            complement(5802..6128)
                     /locus_tag="PK513_01045"
     CDS             complement(5802..6128)
                     /locus_tag="PK513_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81074.1"
                     /translation="MENYTKRIKTRIAQFLNSATAVVVQSYERYLSSDLPKNLDDESK
                     GPAEFKKFHDAGKSAASHLESLLKLADATDDSEQQEKIASRREEQENIQARIQSARAE
                     LEEGDE"
     gene            complement(6260..8281)
                     /locus_tag="PK513_01050"
     CDS             complement(6260..8281)
                     /locus_tag="PK513_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOO81075.1"
                     /translation="MTEHIKMPDVTPIVRYVANGTQDTFTYPFPIFASEDLSVYIDGA
                     KQTSGFTINGAGNTAGGDVTFDTAPAMDTIITLARELPIERVTDFLEGGDFSAQSINN
                     ELDYLIAALQQVSRENDLMLKYGDHETPGNVELPSRATRANKGLGFDGSGNPVALDLS
                     GASASPDFTPTGTGAATRTVNDKLADAISIKDFGAVGDGLTNDTVAIQNAIAAHDMVY
                     IPPGEYLITGTITLTARKALIGAGQKSVLKCNAQTFNAIEITEDHCTVHNFRIEGGDI
                     GIKLYGATRPVVQTSVSDITIVSSNIGVQLDGGANPSFPCYWNNFDRVLVEQFAVHGF
                     HLYRSGAGDTPNANKFHACRAYSLGTTCTGAGFYIEEARYNNAFVDCEANVHGSAQGC
                     FIIGSGCDKTLLINPYAESYNSVPNIKLEAGSIETSIFNLLSASDGAAIWDLSGGEYT
                     AYNAGYPNKNRLQKTTCIDMNATLQRFDTEYINSAGTITLDTSHSVHLVSSFGGALTV
                     NLPAAADATGAMMVVKKIDSSANVITIKEVSGNGPDARDYFLGAENDYMMALSNGAEW
                     FVIASNRSPGNTRYFDGTGTYDIDMAVDTYLLSSFGGALTARLPPANSSQAVGRTVTI
                     KKTDVSANTITVSEQGGTGPDGFAQPLNAQYNAITVVSDGGQWFIVNKF"
     gene            complement(8415..8933)
                     /locus_tag="PK513_01055"
     CDS             complement(8415..8933)
                     /locus_tag="PK513_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOO81076.1"
                     /translation="MGAITPIASSLSAAVSTLNTVGQVVGAVQTLSGNSNARQEQELA
                     LRQLQERQKLQAAGLAQSNALEREKIAAQAAQDEEERRNALRRAVARQRAQFGGSGVS
                     ANSGSAQAVLLGLFDETEDEINRRTQLDSLRTRALDLNETQSRSLNLLQATQLQQRQK
                     LNDQVTLFDRLF"
     gene            complement(9326..11224)
                     /locus_tag="PK513_01060"
     CDS             complement(9326..11224)
                     /locus_tag="PK513_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81077.1"
                     /translation="MTRIREIKTNFTAGEISRELLGRGDLRAYDNGALALRNVFIQPT
                     GGVTRRSGLAYIDTAAGDGKLIAFEFNTQQTSLLVITDEQIDVYTNGAKETTIIAPWT
                     STQIAQLAWTQSADTLLLTHPDAPPKKLTRSGAGTWALEDWSFFTDGGIIAQPYFKFA
                     GSEVTLTPSGTTGSITLTASADVFALGHEGTRLRVHGKEVEITDYDSPTVVTVTTIED
                     LPNTDPTIDWFEQAFSPVRGYPVTVAFHQDRLVIGGSRDLPNRLWFSKSGDLFNFDLG
                     EGLDDESIEFAILSDQVNAIRGIFSGRHLQVFTSGAEWQVTGDPLTPASVQIRRQTRI
                     GSLIDRYIPPTNVDGATLFIARNKQEVQEFLYTDLEQAYKSSDLALLSKHVITGPVDM
                     DYDSRRRLLFLVREDGQFAALTVFRAEAVAAWTLHNTDGAVKSVAVVGDEVYMLIQRG
                     GDYFVEQFDDALNLDSALTGEVGSPATVWSGLDHLEGQSVSIVADGQVVADQTVTGGQ
                     ITLAEAASTIEIGLPYTHIVEPLPPSELGSAGGGRRVRLIEGIFRLQDTQSLRLDIGR
                     GLSDIALRQLGEDEILDAPPPKVSGDISVRSLGWQTDRTKALWRIEQDVPLSFTLLSV
                     TTQLKVND"
     gene            complement(11374..11748)
                     /locus_tag="PK513_01065"
     CDS             complement(11374..11748)
                     /locus_tag="PK513_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81078.1"
                     /translation="MAHFLRNAILTATLTLGTSAALAQDQANDGSAKGNHLSRQFTPC
                     TGGSAAENEACQRVHDAQSEIERQVVKRIGELKASDNWPPKVGIELLERMLKSECRGV
                     KPAKDGQEATEHSPCLPGPFQY"
     gene            12048..12123
                     /locus_tag="PK513_01070"
     tRNA            12048..12123
                     /locus_tag="PK513_01070"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12081..12083,aa:Trp,seq:cca)
     gene            complement(12194..12763)
                     /locus_tag="PK513_01075"
     CDS             complement(12194..12763)
                     /locus_tag="PK513_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81079.1"
                     /translation="MALNDVALASRALIRIGAAPITSFDDGTAESEIAGALYAPVRDG
                     LLSAYPWTFATGQVALTQLSTAPIADYSFAFGLPNDYLRAISAGTGTKGRGLNYRIAN
                     GALHTNASAVVLTYVFLPDESEFPPFFDQALIARLSAEFTIPVTESTSRAEAHFRIAD
                     QEFQRARQIDAQQDTPNKIESFTLVDVRN"
     gene            complement(13050..13289)
                     /locus_tag="PK513_01080"
     CDS             complement(13050..13289)
                     /locus_tag="PK513_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81080.1"
                     /translation="MSFTASDLSVLAYANNFTLWHYVTIDADVTVASYFDKASDMLRV
                     NDLIIANVDTDGTPVTKFYIVTANSGGSVTIAAYA"
     gene            complement(13330..14163)
                     /locus_tag="PK513_01085"
     CDS             complement(13330..14163)
                     /locus_tag="PK513_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HOO81081.1"
                     /translation="MSTTIDQAFIKQFEREVHEAYQRQGSKLRNTVRTISNVNGSSAV
                     FQKIGKGTASTKSTHGMVPVMNLDHSNVEVTLQDYYAGDWIDRLDELKVNIDERQVIA
                     SAGANALGRKTDELIIDSLASASASTIADGNVGMTKDKILEAFETFGEKDVPDDGQRF
                     CVVGWKQWSELLAIDEFVNADYIGADALPFASITQAKMFLGTIFIPHSGLPVDANDIR
                     SCFWYHKTSVGHAAAQDVETDVSWHGDRAAHFVNNMMSQGAGLIDENGIITIDCDETP
                     D"
     gene            14532..14924
                     /locus_tag="PK513_01090"
     CDS             14532..14924
                     /locus_tag="PK513_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO81082.1"
                     /translation="MFGRLFSSLKADNNNEEQSVRRRHARRDCDRCVTVINGQTYPVE
                     NWSLGGVLISSDERQFGLEDEVGVTMRFRLRDNIIDVPHSAEVVRKTKNKVAFRFKPL
                     TQQIRSNFQNVVDDHVASQFAASQSQFR"
     gene            complement(14973..15623)
                     /locus_tag="PK513_01095"
     CDS             complement(14973..15623)
                     /locus_tag="PK513_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81083.1"
                     /translation="MTTNLLIEDIDPEIIPEKFKNPETGALRLDALVNSYAELEKKMS
                     AGPTAPKSPEEYCVNCDHGLFEPDGEVNRRLHAKGMTQEQVQEVYDLAAEKMVPMIKT
                     VATDFSADREVEKLVNHFGGPEAWKEVSRQLLAFGQRNVPADVLENLSSSYEGVLALY
                     KMMKSEEPSLKKQSENPSVLGEQDLQSMMRDPKYWRDKDPAHIAKVTEGFKNVYGE"
     gene            complement(15657..15953)
                     /locus_tag="PK513_01100"
     CDS             complement(15657..15953)
                     /locus_tag="PK513_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81084.1"
                     /translation="MIFDIFKKDTALPMTAAERGLVYTPAEPGKTELREIEKAFARLF
                     SSDDGQKVLAHLQVVTFQRALGPGVGDDQLRYLEGQRAMVATILRLIDRGRKPQ"
     gene            complement(15950..17539)
                     /locus_tag="PK513_01105"
     CDS             complement(15950..17539)
                     /locus_tag="PK513_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HOO81085.1"
                     /translation="MQNTIITKDQPLEPEEIQARLALMLNRFKAAQKKRSNWESLWEE
                     CYNYALPQRGGFTGQPMAGQRKTDQIYDATAMDAADQLASSMLGNLTPTWSQWFGLKA
                     GPDLSDEESERLAPVLEKAAKTIQDHFDRSNFAVEIHQCYLDLIVGGTASLVFEESQP
                     GSFSAFKFSSTPLTHVVLEEGDNGHLDGAFRLMKLTLEQIKSRYPASELPSEIMRRAA
                     RDPQERFKILEAVLPDGLIYSYHACLIEQDDQPVLLSAGSFEQSPMISFRWLKSPGEI
                     YGRSPIMKALPDIKTANKVVELILKNASIAVTGIWQADDDGVLNPANIELTPGSIIPK
                     AIGSKGLQPLDMPGRFDVSQLVLDSLQARIRHALLADRLAPIASPKMTATEVLERSAE
                     MSLLLCATYGRLQSELLTPLIKRAFMILKRRGEIPDIALDGRLVAVDYRSPLARSQGQ
                     KNVQNTLSWISSVLAMGPEASAAINLPQAARFLGDALGVPNDLIRKDIQQIDLSQFIQ
                     PAPVVEPPPVDDTPQQQGDQA"
     gene            complement(17556..17816)
                     /locus_tag="PK513_01110"
     CDS             complement(17556..17816)
                     /locus_tag="PK513_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81086.1"
                     /translation="MGSLSSRSSVPRPTPQVVYVPQTTNTTTSAAESAASAVSVGEMA
                     SQVRTQNLLGRERSRFGTVLTGFRGLLSTLEDHSPRKTLLGE"
     gene            complement(17804..18235)
                     /locus_tag="PK513_01115"
     CDS             complement(17804..18235)
                     /locus_tag="PK513_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81087.1"
                     /translation="MSSHAESQATTKAWVMFSGHADLAWLKILKPGFRHCAVLLNDGH
                     NWITIDPLSNYTDITVHRVPLEFDLPLWMKDRGHKVVPAPINRTPRPAPFAVYSCVEA
                     VKRVLGIHQRLILTPWQLYRYLKRIQTVQTFTPKGDLAWEV"
     gene            complement(18232..18684)
                     /locus_tag="PK513_01120"
     CDS             complement(18232..18684)
                     /locus_tag="PK513_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81088.1"
                     /translation="MSSENKTKTEGCNASHTVPFHNAEEAWFWFIAAQEARTDGARFV
                     AGQSLYPRPCEPLDILKVLDRLYRQRRLLRDHLLVLRHYGRRHLAPDDRRVKEARAFQ
                     LWHEALERIGVVLEKKGIIEPGPSASRDWMQDAMLYENYHLNLEGAVQ"
     gene            19081..19755
                     /locus_tag="PK513_01125"
     CDS             19081..19755
                     /locus_tag="PK513_01125"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO81089.1"
                     /translation="MDKEGIAKKTAQILLNTKSVLFNAREPFTLKSGRASPVYIDCRR
                     LISFPKERRFLMDAGAQMLANDIGVENIDVVAGGETAGIPYGAFVAERLEKPMIYVRK
                     EPKGYGRMAQIEGHLPDKGTPKVVLIEDLQTDGGSKKIFVDALREVGAQVEHAFVVFH
                     YGIFAASEQNMKDMGITLHALATWWDVLAMAKEKNYFDKETLASVDAFLNDPESWEPT
                     HQEGAA"
     gene            19752..20510
                     /locus_tag="PK513_01130"
     CDS             19752..20510
                     /locus_tag="PK513_01130"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOO81090.1"
                     /translation="MKLGFFAGKKPRAQKALEELKSAYGSVPPEEADTIIVLGGDGTM
                     LRALHAFADYQAPLFGMNLGTLGFLLNESRTGGLEERLENAKRFTIHPLVMEAIDRDG
                     QKHNEVAFNEVSLLRETHNSAKIKVHVNDDVRIKELVCDGVMVSTAVGSTAYNSSAGG
                     PILPLQSNIVAMTPISAFRPRRWPGALLHNKSKITFEILQSIERPVSATADSKEVRDV
                     KSVDIHESKKISKTLLFDPENPLQERIFKEQFFS"
     gene            complement(20956..21807)
                     /locus_tag="PK513_01135"
     CDS             complement(20956..21807)
                     /locus_tag="PK513_01135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO81091.1"
                     /translation="MPRRKMIYDGKAKTLYEGPEPGTVIQYFKDDATAGNGAKHDVIA
                     GKGVLNNRISAHLMTKLEGIGIPTHFMRSINMREQLVREVEIIPLEVVVRNIAAGSLC
                     KRLGVEEGTILQRPLVEYYYKKDELGDPMVSEDHIMTFGWADPYELEEMVAMAWRVND
                     YLNGLFSGIGLRLVDFKLEFGRIYGEHGELYIVLADEISPDNCRLWDAKTGEKLDKDR
                     FRFDLGDLIEGYQYIAQRLGLIPEGGIVKGGSINEQLAEKLELIENELAEKRRMRDVK
                     PGKPRKA"
     gene            21946..22272
                     /locus_tag="PK513_01140"
     CDS             21946..22272
                     /locus_tag="PK513_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HOO81092.1"
                     /translation="MSSFDERKDAFENKFAHEEKLDFAVEARCSKLFGLWVAEQFGLE
                     GADAKTYAMEVVESNLEEPGFDDVLRKVKGDLEGKDIEISDHMLNTQLDIALEKARKQ
                     IKEEGA"
     gene            22269..22391
                     /locus_tag="PK513_01145"
     CDS             22269..22391
                     /locus_tag="PK513_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81093.1"
                     /translation="MKSAIIPFPHLNIKKASKISRLLFFPLKPNLAALGCNKIG"
     gene            22642..22881
                     /locus_tag="PK513_01150"
     CDS             22642..22881
                     /locus_tag="PK513_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HOO81094.1"
                     /translation="MAMDAEAIKTMITANIPDAEVRIEDLRGDGDHYAAHITAPCFAG
                     KSRIQQHQMVYGALKGKMGGDLHALSIKTCAPKEG"
     gene            22907..23236
                     /gene="grxD"
                     /locus_tag="PK513_01155"
     CDS             22907..23236
                     /gene="grxD"
                     /locus_tag="PK513_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HOO81095.1"
                     /translation="MQNVLFERIQKELDDHDVVLFMKGEAAFPQCGFSSLVVQVLSQL
                     GVPFKDINVLEDSDLRQGIKDFTNWPTIPQLYVKGEFIGGCDIVREMYESGELQHLFD
                     EKGVKAA"
     gene            complement(23233..24360)
                     /gene="rodA"
                     /locus_tag="PK513_01160"
     CDS             complement(23233..24360)
                     /gene="rodA"
                     /locus_tag="PK513_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOO81096.1"
                     /translation="MRHSIRLHTDQNMFEKLKHLNWGLILLITAVSCIGFAALYSAAG
                     GNFSPWASKQMMRFGIAAIGMVIIAMIDLKWWFRLTWPLYFLGLGLLIIVEIMGHVGM
                     GAQRWINLGFMQLQPSELMKIVVVMALARYFHAATIDDMRRLPFLVIPTLIILAPVAL
                     VLLQPDLGTSLMIVMVGAAMLFMAGASIWLYIGGGVLALASIPVAWHFMHDYQKQRVL
                     TFLNPESDPLGAGYHITQSKIALGSGGINGKGFLEGTQSRLNFLPEKQTDFIFTLWAE
                     EQGLFGGVILLMLFGLILLYGLLIALKSRQNFARYLALGLSVNFSLYVFINIAMVMGL
                     IPVVGAPLPLISYGGTSMLAALIGFGLMMSCSLHPDTKVSR"
     gene            complement(24363..26246)
                     /gene="mrdA"
                     /locus_tag="PK513_01165"
     CDS             complement(24363..26246)
                     /gene="mrdA"
                     /locus_tag="PK513_01165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOO81097.1"
                     /translation="MKKNQDQVRVFTRRAFVIAGLQGALLGVLGGRLAWLQISQGARY
                     KTLSDKNRINLKVLSPVRGEIVDRFGVPLAVNAQNFRALIIPEQAANIEQTLRRLQGM
                     IELSEDDIQAVLKEAGKTAKFVPIEITDDLSWKDVAKIEVNLPDLPGLSTDAGKRRTY
                     PFKEATAHLVGYVGAVSKNDVGDDPLLSLPGFKIGKTGIEKRFDLEMRGSAGQAEVEV
                     NVVGREVRELKKNQSRNGKRITLTIDGELQRFMQGRLSQEKSASAVVMDAHSGAVYAL
                     ASAPSFDPNAFTSRISAAFWEELLANPAHPLTNKAVSGQYPPASTFKMISLLAGLQAG
                     AVTENTTAFCKGHYELGSDRFHCWKRGGHGWVDCRKALQMSCDVYFYELSTKVGIDKI
                     AHVARMMGLGQSLDFELTEERAGLVPDKAWKRGKIGQKWQPGETVVASIGQGYLQATP
                     LQLAVMTARLVNGGYAVKPWVTADGLAKNIMPESGWPKLGISPEHLNLIKTGMDRVVN
                     DEKGTAYGSRAEDPALAFGGKTGTGQVQRITMKQRMSGIKNEELQWKSRHHALFVGYA
                     PLENPRYIASVVVEHGIGGSRTAAPLARDLLIETQKRNPGATPVHGQTQGTASRLKSK
                     EPA"
     gene            complement(26255..26827)
                     /locus_tag="PK513_01170"
     CDS             complement(26255..26827)
                     /locus_tag="PK513_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOO81098.1"
                     /translation="MGPYESLTDKMEFIARMLVPYAVLAMIFILSVVSVPYPLAVFFQ
                     APLLLMAIYYWSVYRPTLLPPWLVFAIGLLLDLITGMPYLGLSAILFLLGRIVVVDQR
                     RFLTGQSFMVIWMGFAALAAIFHIFQWSMSCLLSLQWMPVRDFVSSYVLGIVLFPVLY
                     LFLHLSHKILPAPISRSKTRIGSQKTDMTL"
BASE COUNT         6396 a         6727 c         7169 g         6942 t
ORIGIN      
        1 accctgttcg gtctgaaact cctttttcgc aagaccttgt ctgtaacgcc ggacaaaccg
       61 gaatttgaat ccgaggacat gatcaccgac aagaaaacat ggttttcctc cctggccgtt
      121 ttaggcgtga tggtcgtgct ctttgtcatg cacggcgcgc tgcactggga agcgtgggtc
      181 gtggcctgcc tggggctgac ggttttactg gctctgacct acaaagccaa tccggacaaa
      241 tacctgacgg aagtcgagct ttcgctgctc atgttcttcg tttccctgtt cgtaatcgtc
      301 ggcggggttg aaggctcgca gtttctggaa tggatcggtc agttcatcac cccgatcgta
      361 cgtgaagaca tgctgctggc ctgtctggtc ctgatgtggg tcgcggcaat cctttcggcc
      421 atgatcgata acatcccgtt cacagcagct atgatcccga tcatcatggg catggaggcg
      481 caaggcattc cagtcacacc attatggtgg gcgttggcga tcggcgtagg tatgggcggt
      541 aacggttctc acctcggctc taccgcaaac gtgttcatcg tgacactgag cgaacgccta
      601 gcccaacagg agaacaagcc ttctttgcgc ataacgccag gcgtatggtt tcgcaaaggt
      661 acgccggtca tgctgttgac gctgtgcgtt tgttcgctgg cgatgtggct ctttttcccc
      721 tttttcgctc atcccctgcc gacggcggag atgcacgcgg ccaagatgca tgaaaaagcg
      781 gctcacgcaa tggaaaaaac ggagcatcac ggttagattg cacgcatgat tgcggtataa
      841 agctttctct taaaaaagcc cggcatttcc ccgggctttt ttttatgata taagaagcat
      901 caaatgcgaa tagccagtta cactggcgtg atctttaccg cttgatactt ttggggaacc
      961 cacacatgct caaacgcgcc ggatatttat ctcttttaat cgtcgctaca ttatttgctg
     1021 tttgccttcc taccgcctcg atttgggcgg catcaaacag caatgcctcg gcccaaaccc
     1081 tgaacgctgt tacgcacccg gcggcaattt tgtgtctggt tattttcgtc ttgtcttatg
     1141 ttgcggtgtt gatggaagaa tacacccaca tgcgtaaatc caagcctgtg ctgttgggcg
     1201 ccggcttgat ctgggtcatt atcggcatga tcgcgccgtc ttacggcatt gaccacgata
     1261 cccttcatag cgcgattttt cacgggctgg aagaatatgc gagtttgctg ctttttcttc
     1321 ttgccgcaat gacttatatc agcgcactgg aagagcgcaa tgtttttgcc gtattaaaaa
     1381 gcaacctgat ccggtcgggg ctgaatttta gacagctttt ctgggcgaca ggacttatcg
     1441 ccttcttttt atctccagtg gcggacaatt taaccacagc attggtgttg ggggctgtgg
     1501 ttgtggccgt gggtagcaat aatccgcgct ttgtttctct ggcttgtatt aatgtggtta
     1561 gtgctgccaa tgcagggggg gcgttcagcc cgtttggtga tattacaacg ctgatggtct
     1621 ggcagtccgg gaacgtctcc ttctttgaat tctttaattt attccttcct tccgttatct
     1681 gcttcctgat accggctgcc attatgagcg ttttcattcc gcgtgattat ccggaagccc
     1741 tgaaagaaga cgtatcgctt cgccatgggg cgcggatgat tatcgccctg ggagttttca
     1801 ccattgccat ggccgttggg ttcgaacaga cacttggtct gccacctttt gtcggcatga
     1861 tgtttggtct gtctttcttg atgatcgctg cttacgtcat ccggcacgtg tgggtcaaca
     1921 aagacgaaga taaggatttt gacattcttg aaaatgtcgc ggcggcggaa tgggatacgc
     1981 tgctcttctt ctttggtgtg atcttctctg tcggcggcct ggccttttta gggtatatgg
     2041 agctggcctc tgctgcgatg tatcaggagt ttggcgcaag cgcgacaaat atcgttttgg
     2101 gggtatcttc tgccataatt gataatattc cggtgatgtt cgcggtgctg agcatgaatc
     2161 cggaaatgga tcatttccaa tggctgctga ttactctgac cacaggcgta ggtggcagca
     2221 tgctttctat tggttctgcg gcgggcgtag cgttgatggg tgtggcgcgt gggcaatata
     2281 catttatgag ccacctgaaa tggacgccgg ttatcgtttt gggatatatc tcggcgatcg
     2341 gtgtacattt cctcattaat tctcatttaa tgaatgtttc gatccatgca attgatgtta
     2401 cgacacatat tgaggaacct gcaccaacac attaacgcga tgcattccaa aagatggttt
     2461 cgggtgagac aagcttttta tcttcactac gtttataaac gtaaataccc aacacagaaa
     2521 ggccgacagc ccagattgcg ctgagcgatg acatagacga cattatatat ccggcacgtt
     2581 gcgtatcgaa tatcataatg tatgcaaccc ccagcatttg cgcggcccag gtgatggcca
     2641 tcaggtagcc gaaagtggga cgcatccgcc gaatgtattt atcagaggag gcaatttcgg
     2701 tgcgcagcga ttcattaacc tgtttaaaag cttcacggta ttcctctgat ttcattagag
     2761 aaagtgcctc catgtgcctg ttcgattccg ctatagcttc cggtgaaata tctcctgctt
     2821 tcagcgcttt atcaacatct tctaaagctt ttgccgcact tttggtgacg ggcgtgttga
     2881 tctgttttaa ggctcctgat acgaaggtga tcagcagcgg tagaccgatg cgggcgagta
     2941 tggtttcgag catggatcag gcctcctgca atttgtaaaa gatgtgatgg ccgattttgg
     3001 cactggggat ttcatcgcgt gcccaatatg gcgaggtata atcggcatgg taatgggtag
     3061 ccccaccagt atgatccggc aaaaccccgg ccacggcacg ccgggcaatg cgcagggcgc
     3121 tggcgaaata aaggttttta tcatcgatgg cctgtaattt ctgaaaattt gggtcagagc
     3181 gattccagca cgagaattga tacggttttt gacagacctg aatgatattg cctccccacc
     3241 agtatttacc gccatgacct tgcgcgactt tcacgcggtt gagcacaaca ctggcgaccg
     3301 cttccattcc aatatgaccc tgtccccggg cttcacccca gagagtgcgc gccagaacat
     3361 caatttcaag tgcgcggtaa aattcttcgg cgtcagcgcg cgggcgcacg tctgaaccac
     3421 caattgcagt cagggttggt tttttcattg tgttctcctg taattaagtt ctgcgtcatt
     3481 gcgggcaccc tctggggcat aaacttcgtg aagcaatcca tctcttacgc gaatttcaga
     3541 gctggattgc cgcgtcaggc cttacggcct tcctcgcaat gacgacgggg gttattcaga
     3601 tttggcctta agagcctcgg ccttaagagc ggtggcgtcg agtttggatt cgatgcgcag
     3661 caaatgctcc accagccggg attcgagcgc ccgtaaatcg gtctgcgagg cataagtttt
     3721 ggcaacttca agtttgaatc cgctgagcgc atcgcggagc tgatccgagc ggtgctcaag
     3781 catgtgctct aaattgttga tggctctttc ggcttcgcgg cgcgtccgcc aggaaaaagc
     3841 caaaaggccg gccatggccg gcagatcgaa aattgtgatc caccagacca gatcttgtga
     3901 aagcaggttc ataatttccc ttatcttttt tcttgtataa aatgttttgt tatgttaatt
     3961 tttggctact tttttaaagg ggattaaaat gcctaatacg tccgtgcttg ttcccggtga
     4021 caaacgaaca aaagaatttc tgagccgtgc gcatggctat gtccaagtcg cgaaagacgc
     4081 aggccttcct tttaatgacc aacttcagga agccgttgat ttcatggaga tccattcaca
     4141 aggctccagc cctttgagtt tccgtgttct tcaagcctct tttaaccgcc attgcggtcg
     4201 ggaggcacaa cgctggatca attgttttaa aagagaactc gtttattgca ccacactgcc
     4261 tgctaactgt actcccggcg accactccta aactctgaaa tcagtgtccg ccttgtgagt
     4321 ccgggcggat ttcatccagt tatggccgcc acggccgtag aggcgcggca ggcggtcagg
     4381 cgcttgggaa agcgcacctg caacggcatc gagtccatca tcatgccctt tattactttc
     4441 gggccgccat tcgcgcattt cagtcatgaa gggcgttctt aggatcgatt tgtgaatgtg
     4501 caaccgccgg gcggcgagca cggcatcgaa ggcctccaaa atacgcagat ctttggcacg
     4561 gctctggctg gtttcctgta caccgcacgg cgctttggcc ctggcaagct cattgcgcaa
     4621 gatgccggga agaaaccgcc cgatgccgtt cgtctcaact gtcatggcgg gcaggtaatg
     4681 atttttagca atctgcgaaa cgatacggca ttgttgcgtt gcttcgtcct gctcattacc
     4741 agcgtcggtg cgaatatatt cgatgtgatg aaggtagaaa ttcccctccg catcgctaaa
     4801 tatgacggcc ataacgctgt ggtcgccttt agctgcgccg aaggccgggt cccaaaaggc
     4861 gctgacggag accatctttg tttggcccaa aaacagggtt tgcagttcct tacagtaatc
     4921 cagcggttca tcataatggc gcagcagatc gggatttaat cgcccttcgg caatgttcac
     4981 gggctgaagc atcatctggg aggcgaattt attcggcccg gcggccctct tcataccggc
     5041 gatatcggcc tcgctatagc gctcaatcca tgcgctttgc cctttttcgt ccagaatagg
     5101 aattttgaga cgttcaaagc cgtttaagaa ttctttttcc tcgcccagtt gcgtacgcgg
     5161 ctcatcagcg tagatcgtgt ggtaattatg cggcgtacca acgtaaattt tcgttccgcc
     5221 ggggacgagg acatattcca tctcgcccag gcgctcgcgc aattcgcggc gtttttcggc
     5281 gctgtcgcag gtgttgggaa cctcgacatc atcgcaaatg acaatatcgg cgcgtgagcc
     5341 ggtgatgttg gcggtgatgc ccttggccat catggagggg tcgcgcgctt caaaagtgcg
     5401 tttgaccgtg aagcggtcgg aggcccattg gtcggcgttt tccggtttca tttttgtggt
     5461 cagcgggtgg cgctcgataa tgcgccgcac gttgcgcacc atctttttcg ccagcgtaaa
     5521 atccgccgcc aacacaagga tacgcaaatc cgccttgcga tataaaagcc aggccgaaaa
     5581 caggccggtg atcgtgcttt tgcctgcgct gcggaaggcc atcaggagaa gccgtgtatt
     5641 cttgcgggtc caattccatt ccagccagcg cgccattttc aggtggattt gtggtgtgcg
     5701 cagccccatg cgctggttcc acagcaccac gaagagcggg aaatcggctt cgctgatgtc
     5761 gaatttcgtg ctttgcaggc tggtcggctt tacgatttcg ttcattcatc gccctcctcc
     5821 aattctgcgc gggcgctttg tatccgggct tgtatgtttt cctgctcttc gcgcctggac
     5881 gcgatttttt cctgctgttc gctgtcgtcc gtggcatcgg ccagtttgag gagactttcg
     5941 agatgggagg cagcggattt cccggcatcg tggaattttt taaattccgc cgggccttta
     6001 ctttcatcat caagattttt tggcaaatcg ctgctgagat aacgttcgta ggattgcacg
     6061 accactgccg tggcgctgtt gagaaattgt gcaatgcggg ttttgatgcg tttcgtatag
     6121 ttctccatat aatttccttg ttggtttttg aaccacaatg tggtcccttg acgtcattgc
     6181 gagcgacagc gaagcaatcc atctatagga gttggattgc cgcgtcaggc cttcggcctt
     6241 cctcgcaatg acgcataaat taaaatttat tgacgatgaa ccactggccg ccatcagaaa
     6301 cgacagtgat ggcgttgtat tgcgcgttta agggttgagc gaagccgtcg gggccggttc
     6361 cgccctgctc gctcaccgtg atggtgttgg ccgagacatc ggtttttttg atggtcacag
     6421 tgcgcccgac agcctgggag gaattggccg ggggcagacg agcggtgagc gcaccaccaa
     6481 agctggagag caaataagta tctacggcca tatcgatgtc ataggtgcca gtgccatcga
     6541 aatagcgggt attgccgggg gagcggttgg aagcaatgac aaaccattcc gcgccgttgg
     6601 acagcgccat catgtaatca ttttccgccc ctaaaaaata atcgcgcgca tcgggaccat
     6661 tgccgctgac ttccttaatg gtgataacat tcgcggagct gtcaattttc ttaacgacca
     6721 tcatcgcgcc cgttgcatcg gcggcggcgg gcagattgac cgtcagggcc ccgccaaagc
     6781 tggaaaccag atgcacggag tgtgaagtgt cgagcgtgat ggtcccggcg ctattgatat
     6841 attcggtatc gaagcgctgg agcgttgcgt tcatgtcgat acaggtggtt ttttgcaggc
     6901 ggttcttatt cggataccct gcgttatagg cggtgtattc gccgcctgaa agatcccaga
     6961 tcgccgcgcc gtcgctggcg gagagtagat taaagataga ggtttcaatg gagccggctt
     7021 cgagtttgat attagggacg ctgttatagc tttcggcata ggggttgatg agcagcgttt
     7081 tatcgcagcc agacccgatg atgaaacagc cttgcgcgga gccgtggaca ttggcttcgc
     7141 aatcgacaaa ggcgttatta tagcgtgctt cttcgatata aaacccggcg cccgtacagg
     7201 tcgtgcccag agaataggcg cggcaagcat ggaatttatt ggcgttgggc gtatcccctg
     7261 ccccgctgcg atagaggtga aagccatgga cggcgaactg ttcaaccagc acgcggtcaa
     7321 aattgttcca gtaacatgga aagctcgggt tcgcgccgcc atcaagctgg acgccgatat
     7381 tggacgaaac gatggtgatg tcgctcacgg acgtttgcac gaccggacgc gttgcgccgt
     7441 aaagcttaat tccgatatct ccaccttcaa tgcggaaatt atgcacggtg caatgatcct
     7501 cggtgatctc aatggcgttg aatgtttgcg cgttgcattt gagcacggat ttctggcccg
     7561 cgccgatcag ggctttgcgc gcggtaaggg tgattgttcc cgtgatcaga tattcgccgg
     7621 gcgggatata aaccatatcg tgcgcggcta ttgcattttg gatagcgacc gtgtcattgg
     7681 tgaggccgtc accaacagcg ccaaaatcct tgattgagat cgcatcggcg agcttgtcat
     7741 tcacggtgcg cgtggccgcg ccggtgccgg taggggtaaa atccggcgag gcggaagcgc
     7801 cggagagatc gagcgcgacg gggttgccgc ttccgtcaaa gcctaggcct ttatttgcgc
     7861 gggtggcgcg cgatggcagc tctacattgc cgggggtttc gtggtcacca tatttgagca
     7921 tgaggtcgtt ttcgcggctg acctgctgga gcgcggcgat gaggtaatca agctcgttat
     7981 tgatggattg ggcggagaag tccccgcctt cgagaaagtc ggttacgcgc tctatgggca
     8041 gctcgcgggc gagcgttatg atggtatcca ttgcaggagc ggtgtcgaaa gtgacatcgc
     8101 cgcccgccgt attccccgcg ccgttaatgg tgaaaccgct ggtctgcttc gcgccgtcta
     8161 tatagacgct caagtcctcg ctggcaaaaa tgggaaacgg gtaggtgaat gtgtcttgcg
     8221 tgccattagc gacataacgc acaattggcg ttacgtccgg cattttgatg tgttcggtca
     8281 tgaagggagg cttccttaaa actggttttg tatctgtcat tgcgagtacc cacagggcat
     8341 aaacttcgca aagcaatcca gttgatatgg attgccgcgt cgaacctatc ggttctcctc
     8401 gcaatgacgg cgggttaaaa caagcggtcg aagagtgtga cctgatcgtt gagcttctgg
     8461 cgctgctgga gctgggtggc ttgcaggagg ttgaggctgc gggattgcgt ttcattcaga
     8521 tcgagtgcgc gggtccgtaa agagtcgagc tgtgtacgcc gattgatctc gtcttcggtt
     8581 tcgtcgaaca ggccgagcag gacggcctgg gccgatccgc tattggcgct gacgcctgat
     8641 ccgccaaatt gcgcgcgttg gcgtgcgacc gcccggcgca gagcgttgcg gcgttcttcc
     8701 tcatcttgcg cggcctgcgc ggcgattttt tcgcgttcca aagcattgga ctgggctagc
     8761 cccgcggcct gcaatttctg gcgctcctgt aattgacgca gggcaagttc ctgctcctgg
     8821 cgggcgttgc tgttgccgct taaggtctgg accgcgccga cgacctgacc gacggtgtta
     8881 agtgtactga cagcggcgga caggctgctg gcgatggggg taatggctcc catgggaacc
     8941 tcctttccga ttattgtgaa taattttata tttttgaatt aatcttgcgg ttttacggtt
     9001 gtttttttta taaaaaatct ttacggtatt taaaatacat cgaatgctct tttttatatt
     9061 gacacacaga acattattat ggtaaataat ttgcatccta taaccttaag agaggaaaaa
     9121 acgatgacat ggacaagtgg acgccttcct agctgtgtga gttgtgacaa aattgaaacg
     9181 cccgttagag taaacgatgc ttttgacaga aattctcgga caaaagccat tcaaaagcct
     9241 gcgcgcacaa tctgggcttc ctcccctggg agtgatcaag aagaagcggc aacaacgaag
     9301 cttatagctg aactacatta ataagctaat cattaacttt taattgcgtt gttacggaca
     9361 gcagggtgaa gcttaacggc acatcctgct cgatccgcca cagggccttc gttctatcgg
     9421 tttgccagcc gagactgcgg acgctgatat cgccgctgac ttttggcggc ggcgcatcga
     9481 gaatttcatc ttcgccgagc tgacgcaggg caatgtctga taatccccgt ccaatatcaa
     9541 gccgcaggct ttgcgtatcc tgaaggcgga aaatgccttc aatcaggcgc acacgccgcc
     9601 ctcctccggc gcttcccaat tcgctgggcg gcaggggctc aacgatatgc gtatagggca
     9661 gcccgatctc gatggtgctg gcggcttcgg cgagtgtgat ctgcccgccg gtgacggttt
     9721 gatcggcaac gacctgcccg tcggcaacga ttgaaacgct ttgcccttca agatgatcaa
     9781 ggccggacca tacggttgcg ggactgccga cttctccggt caatgcagag tcgagattga
     9841 gcgcatcgtc gaattgctct acgaaataat cgccgccgcg ctggatcagc atatagactt
     9901 catcaccaac gacagcaacc gatttcactg cgccatcggt attatgcagg gtccaggcgg
     9961 caacagcctc agcgcgaaaa acagtgagcg cggcaaactg accgtcttcg cgaactaaaa
    10021 ataacaagcg gcggcgtgaa tcgtaatcca tatcgacggg gccagtgatc acgtgctttg
    10081 agagcagcgc gaggtcagat gatttataag cctgctccaa atcggtatag aggaattcct
    10141 gcacctcttg tttattgcgc gctataaaga gcgttgcacc atcgacattg gtcggcggga
    10201 tatagcgatc aatcagcgaa ccgatgcggg tttgcctgcg gatctggacg ctggcggggg
    10261 tgagcgggtc gccggtgacc tgccattccg cgccggaggt gaagacctgt aaatgccgcc
    10321 cggaaaagat accgcgaata gcgttgacct gatcggacag gatggcgaac tcaatagatt
    10381 catcatcgag gccttcgcca agatcgaaat tgaacagatc gccgcttttg gaaaaccaca
    10441 ggcggttggg caaatcgcgg ctgccgccaa tgacaaggcg gtcctgatgg aaagcaaccg
    10501 tcaccggata tccccgcaca ggggaaaagg cctgctcgaa ccagtcgatt gtgggatcgg
    10561 tatttggcag gtcttctatg gttgtgactg tcacgaccgt gggggagtcg taatctgtga
    10621 tttcgacttc tttaccatgt acgcgcaggc gagtgccttc gtggcccagc gcaaatacat
    10681 cagccgaagc cgtgagcgta atagagcctg ttgttccgct cggcgtgagg gtgacttcgg
    10741 agccagcgaa tttgaaatag ggctgggcga tgatcccccc atcggtgaag aaactccagt
    10801 cttccagcgc ccatgtgccc gcgcccgagc gggtgagttt tttcggcggc gcatccgggt
    10861 gggtaagcag cagcgtatcg gcgctttgcg tccaggcgag ctgcgcgatt tgggtactcg
    10921 tccacggcgc gatgatggtg gtttctttgg cgccatttgt gtagacatcg atctgctcat
    10981 cggtgataac cagcagcgaa gtttgctggg tgttaaactc aaaggcaatc agcttgccgt
    11041 cgccggcggc ggtgtctata taggccaggc cagaacggcg cgtcacgccc ccggtgggct
    11101 ggataaaaac attgcgcagg gccaaggcac cattgtcata ggcgcgcaaa tccccgcgtc
    11161 ccaaaagctc acggctgatc tcaccagcgg tgaagttggt tttgatttca cgtatgcgtg
    11221 tcatggtttt tcctttaaat ttaaggtgtc atgcccgctt tacgcgggca tccggatccc
    11281 cgcataaagc ggggatgaca aaaatggggc aacaaaaaac ccgccgctct tttgccgagg
    11341 ggcgggtttg gggaatttaa cttctaaaaa ttttcaatac tgaaaaggcc caggtagaca
    11401 tggagaatgt tctgtagctt cctgaccatc ttttgcgggt ttaactcccc tgcattccga
    11461 ttttaacatt ctctcaagaa gttctatgcc aactttagga ggccaattat cggatgcttt
    11521 caactcacca attcttttta cgacttgtcg ttcaatctct gattgtgcat catgcactct
    11581 ttggcatgcc tcattttccg cagccgatcc tcctgtgcaa ggggtaaatt gtctggatag
    11641 atggtttcct ttggcgctgc catcgttagc ctgatcttga gccaaagctg cgcttgtacc
    11701 aagtgtcaac gtggctgtta aaattgcatt tctcaaaaaa tgcgccatta cgctctccgt
    11761 gctgtttatt acgatgaggc acgctagcac atatgaagca cgaagtcaat aattatgtta
    11821 aattattatc cccgtaatca acaagcgtga agctttagat tttgttgggg ggtgtcttgc
    11881 tacacatcga tttggcgggc gcgttggaat tcctgatcgg cgatgcggaa atgtgcctcg
    11941 gcgcggcaat ccatctctag atcgctttgg taataaaaat ctctgtctta aaaggagcaa
    12001 tgcttgactt tttgtccctt aatcctttat ctgtgaagtc cgtttaaagg ggtatagctc
    12061 agttggtaga gcgacggtct ccaaaaccgt aggtcgcggg ttcaagtcct gctgcccctg
    12121 ccatttcagc ccggcgtaaa aattctctta tcgaataaca ggggcgtgaa tccatcgatg
    12181 ttatgtccaa tatttaattc cgcacatcga caagggtgaa gctttcgatt ttgttggggg
    12241 tgtcttgctg ggcgtcgatc tggcgggcgc gctggaattc ctgatcggcg atgcggaaat
    12301 gcgcctcggc gcgggatgta ctttccgtca cgggaattgt aaattccgcc gatagtcttg
    12361 cgatgagcgc ctgatcaaaa aacggtggaa attcactttc atcaggaaga aaaacatagg
    12421 tcagcacaac ggcgctggcg tttgtgtgca gcgcaccgtt ggctatgcga taattgaggc
    12481 cgcgtccttt tgtgccggtt ccggcactga tcgcgcgcag ataatcgtta ggcaggccga
    12541 aggcgaaact gtaatcggcg atgggggctg tgcttagctg ggtcaacgcg acctggccgg
    12601 tggcgaaggt ccacggataa gcagagagca ggccatcgcg cacaggggca tagagcgcgc
    12661 cggcgatttc ggattcggct gtgccgtcgt caaagcttgt gattggcgcg gcgccaattc
    12721 ttataagtgc gcgcgaagcc agcgcgacat cattgagagc catgggaaat ctccaaattc
    12781 tttatgtgtt aaagggtggg tgcagccccg cctttgccat gaagaggaaa ggcaagagcg
    12841 tggactgcac ccttgcgaag cgagtccaga gtagttgttt gcgtctaaaa gaaacgaaca
    12901 aaatctccgg ttttgcttca tgatcgatac ttcctgaaac tcactaaagc cgggcttggc
    12961 gagcgcattt gtgcaagccg gataatggcc agcgctcaaa gccttaagca gtgttttaca
    13021 acagagagaa aacgatccgc agctatgttt tacgcatagg ctgcgatcgt gacactgccg
    13081 ccggaattgg ccgtgacgat gtagaatttc gtcacagggg ttccatcggt atcgacattc
    13141 gcaataatca gatcattcac acgcagcata tcggaagcct tatcaaagta agaagctacc
    13201 gtgacatctg catcaatggt gacatagtgc caaagtgtga aattgttggc gtacgcgagc
    13261 acgctcaaat cagatgctgt aaaagacatt gatttatctc cttaatgttt gaagttgtgt
    13321 actgcgcgat tagtcgggtg tttcatcaca atcgatggtg atgatgccgt tttcgtcaat
    13381 caaacctgca ccctgactca tcatgttatt gacgaagtgg gcggcgcggt cgccatgcca
    13441 cgagacatcg gtttcaacat cttgggctgc ggcgtgaccg acggaagttt tatgatacca
    13501 gaagcaagag cggatatcgt tggcatcgac cggcaaaccg gaatgcggaa taaagattgt
    13561 tccgaggaac atcttggctt gtgtgatgga cgcaaatggc agcgcatcag caccgatgta
    13621 atctgcgttg acaaattcat cgatggcaag caattcgctc cattgtttcc agccgacgac
    13681 acagaaacgc tggccatcat caggcacatc tttttcaccg aaagtttcga aagcttcgag
    13741 aattttatcc ttggtcatgc cgacattgcc atcggcaatc gtgctggccg atgcgctggc
    13801 caaagaatcg atgatcagtt catcggtttt gcgacccagc gcgttagcac cggcgctggc
    13861 gatcacctga cgttcatcga tattgacttt gagttcatca agacggtcga tccagtcgcc
    13921 tgcataatag tcctgcaatg tgacttcgac gttggagtga tccaaattca tcaccggcac
    13981 catgccgtgg gtggatttgg tggaggcggt gcctttgcca atcttttgaa agaccgctga
    14041 tgaaccgttg acattggaaa ttgtgcgcac tgtgttccgc agtttggaac cctgacgctg
    14101 gtaggcttca tgcacttcgc gttcgaattg cttgatgaaa gcctgatcta ttgttgtaga
    14161 cataggcgat aaccttttct tttaaagttg ctaaaggagt ttgaggcccg gccctcaaaa
    14221 caggttgtcg ctcagcgcag tgaacggcca ttatcacggg acccgcaagg catatttatg
    14281 ctattacagc accttatgtt gtcctggggt gaattcttat aaagacgcca cgggatagac
    14341 tcctggcgtt gattgaatat attgtaggaa tttattcctt tattgtcaag gagaaattaa
    14401 gcccgtattt gacttttttc taacacaagc cctataattg ccataattat gtggatacta
    14461 gacactgtat agaattgtaa tttttactaa aaacgcttag actgtgctct gagaggacag
    14521 ggatataaaa aatgtttggt cgattatttt catcacttaa ggctgataat aacaacgaag
    14581 agcaaagtgt gcgcagacgt catgcgcgcc gtgattgcga tcgttgcgtt acggttatca
    14641 acggacaaac ttacccggtt gagaattggt ctttgggcgg cgttcttatc agttccgacg
    14701 aacgccagtt cggccttgaa gatgaagtcg gcgtgacaat gcggtttcgt ttacgggata
    14761 atatcattga tgtaccccac tccgccgaag ttgtgcgcaa gaccaaaaac aaagtggcat
    14821 tccgattcaa gcctttaaca caacagatac gcagtaattt tcaaaatgtc gttgatgatc
    14881 acgttgcttc ccagtttgcc gcctcgcaaa gtcagtttcg ttaaacgctt taaaaaggga
    14941 cgcgattaaa tccgcgcccc atgtcttaat ttctattcgc cataaacgtt cttaaagcct
    15001 tcggtaacct tggcgatatg cgcagggtct ttatcgcgcc agtattttgg gtcgcgcatc
    15061 atggattgaa gatcctgctc gcctagcaca gaaggatttt cgctttgctt cttgaggctt
    15121 ggctcttcag acttcatcat tttatagagc gcaagcaccc cttcgtagga actggaaaga
    15181 ttctccaaaa catcggcagg aacatttctc tgaccgaacg ccagcaattg acgcgaaact
    15241 tctttccatg cttccgggcc gccgaaatga ttcacgagtt tttccacttc gcgatcggcg
    15301 ctgaaatccg tggcgacggt cttgatcatc ggcaccattt tttctgcagc caagtcatag
    15361 acttcctgca cttgctcctg ggtcatacct ttggcgtgga gacgccggtt cacttcgccg
    15421 tcgggttcaa acaggccatg atcacaattc acacagtatt cttccggtga ttttggtgca
    15481 gttggaccgg cggacatttt cttttccaat tcggcatagg aattgaccag cgcatccagg
    15541 cgaagtgcgc cggtttccgg gtttttgaat ttctccggaa taatctccgg gtcgatatct
    15601 tcgataagta ggttggtagt catggttttt agtctcctta tttgacagtc agaaaattat
    15661 tgcggcttac ggccacggtc gatgaggcgt aaaatcgtgg caaccatggc acgctggcct
    15721 tcgaggtagc gcagttgatc gtcaccgacc cccggcccca gagcccgttg aaacgtcacg
    15781 acctgtaagt gtgccagcac cttttggccg tcgtcggatg aaaacaggcg tgcaaaagcc
    15841 ttttcaattt cgcgcagctc cgttttaccc ggttcagccg gggtataaac cagaccgcgc
    15901 tcggcggcag tcatgggcaa tgcggtgtct tttttaaaaa tgtcgaatat catgcttggt
    15961 ctccttgttg ttggggcgtg tcatcaacgg gcggcggctc aacgactggc gcgggttgta
    16021 tgaactgtga cagatcaatc tgctggatat ccttgcggat taaatcgttg ggtactccca
    16081 gcgcatcgcc gaggaaacgc gctgcttgcg gcaggttaat tgcggcgctg gcctccggcc
    16141 ccatggccag tacggaagaa atccagctca gcgtattttg gacattcttt tgcccttgag
    16201 agcgcgcaag cggtgagcgg taatcgactg cgacaaggcg accatcgagt gcaatatcag
    16261 ggatttcacc gcggcgctta aggatcataa aggcacgttt tatcaacggc gttagaagct
    16321 cggattgcag acgaccatag gttgcgcaca gcaaaagcga catttcggcg ctacgctcca
    16381 gcacttcggt agctgtcatt ttgggcgaag caatcggcgc gagacgatcc gcaagcaagg
    16441 catgacggat gcgagcctgc aaactatcga gaacaagctg tgagacatca aagcgccccg
    16501 gcatatcaag aggctgcaat cccttggagc caatagcttt ggggatgatg ctgccgggag
    16561 tgagttcaat attggctgga ttaagtacgc cgtcatcgtc ggcttgccag attccggtca
    16621 cggcaataga ggcgtttttc aggatcaact cgacgacttt gtttgcggtt ttaatgtcgg
    16681 gcagcgcctt cataatcggt gagcgaccat agatttcgcc agggcttttc agccagcgga
    16741 aagaaatcat cggcgattgt tcgaacgacc cggcgctgag caagacaggt tgatcgtcct
    16801 gttcaatcaa acacgcatga tagctgtaaa tcagcccatc tggtaggacg gcttcgagaa
    16861 ttttaaaacg ctcctgcgga tcgcgcgcgg cgcggcgcat aatttcagag ggaagctcag
    16921 aggccggata acgagattta atttgctcca gcgtgagttt catcaaccgg aatgcaccgt
    16981 cgagatggcc gttatcgccc tcttccaaca ccacatgggt gagcggcgtg gaggaaaatt
    17041 taaaggctga aaaactgccg ggctgtgatt cctcaaacac tagcgaagcg gtgccgccga
    17101 cgataagatc aagatagcat tgatggattt cgacggcgaa gtttgaacga tcgaaatgat
    17161 cctgaatggt cttggctgct ttttcgagca caggggcgag gcgttcagat tcctcatcgc
    17221 ttagatccgg gccggccttc agaccaaacc attgcgacca tgtcggcgtc aaattgccga
    17281 gcatgctgga agccagttga tcggcggcat ccatggcggt ggcgtcataa atctgatcag
    17341 ttttgcgctg acctgccatt ggctggccgg taaaaccgcc gcgttgcggc agagcgtaat
    17401 tataacactc ctcccacagg ctttcccaat tgcttcgttt cttttgcgcg gctttgaagc
    17461 gatttaacat gagcgccagc cgggcctgaa tttcttcggg ttccaaaggt tggtcttttg
    17521 ttattatagt gttttgcatt ggttaaacct ctttcctatt cgccgagcaa agttttacgc
    17581 ggactatgat cttcgagtgt gctgagcaga ccgcgaaagc cagtcagaac ggtgccgaaa
    17641 cggctgcgtt cacgacccag taaattctgg gtgcgtacct gcgatgccat ttccccgacg
    17701 cttaccgcgc tggcggcact ttcagccgcg cttgtggtcg tgttggtggt ttgcggaaca
    17761 tagacgactt gcggcgttgg ccttggcaca cttgaccttg aacttaaact tcccatgcta
    17821 aatctccttt cggggtaaat gtctgaacgg tttgaatgcg ttttaaatag cgataaagct
    17881 gccagggcgt caggatcagg cgctggtgaa tccccagcac acgtttgacg gcctcgacgc
    17941 agctataaac agcgaaaggc gctgggcgcg gcgtgcgatt aatcggtgcg ggcacaacct
    18001 tatgaccacg gtctttcatc cagagcggca gatcgaattc cagcggcaca cggtggacgg
    18061 tgatgtcggt ataattcgac agcggatcaa tggttatcca gttgtgaccg tcattcagta
    18121 aaactgcaca atgacgaaaa cccggtttga gtattttcag ccatgctagg tcggcatggc
    18181 cactaaacat gacccaggct ttggtcgtgg cttgtgattc agcatgcgaa ctcattgtac
    18241 ggcgccttct aaattcaaat gataattctc atagagcatg gcatcttgca tccaatcccg
    18301 gctggcgctg ggcccaggct cgatgatgcc tttcttttcg aggactacgc ctattcgctc
    18361 taaagcctcg tgccagagct ggaatgcgcg ggcttctttg acgcggcgat catccggcgc
    18421 caaatgccgc cgcccgtaat gacgcaggac cagcaagtga tcccggagca aacggcgctg
    18481 gcgatacagc cggtcgagta ctttcaaaat atcgagcggt tcgcaaggac gcggatacag
    18541 gctttgcccg gcgacaaaac gtgcgccgtc ggtgcgagct tcctgcgcgg cgataaacca
    18601 gaaccaagct tcttccgcat tgtgaaatgg tacagtgtgc gaagcgttgc agccttctgt
    18661 ttttgtttta ttttctgaac tcatctgcat tcttccccct tattttagat ataactgttc
    18721 cctttatgtt ctcgtttgtg ctataaattt tgttatgagt caagaataaa ataagaattt
    18781 attcctattt atttttgcgc gtgcaaatgg gactattgtc ctatgttgac tcacaagaaa
    18841 atctggctgg cgatcgacag actggccaat gcatttggtt actccccatc gggactggcc
    18901 aagcaggccg ggctagaccc cacgtctttt aataaaagca agcgccatag cccagatgaa
    18961 aaaccgcgct ggccttcgac ggaaagtatc tctaagatcc tgtctgtaac gggcgcgacg
    19021 atgagtgaat ttatcgcgct gattgatgat gatgacgaac actataaggc catatcggcc
    19081 atggacaagg aaggaatagc caaaaaaacc gcacagattt tgctcaacac aaaatcggtt
    19141 ttatttaatg cgcgcgagcc tttcacactc aaatcaggcc gtgcaagccc ggtatatatt
    19201 gattgccgcc ggctgatttc ttttccaaaa gagcgtagat tcctgatgga tgcgggcgcg
    19261 cagatgctag ccaatgacat cggcgtagaa aatatagacg tggttgcagg cggagaaaca
    19321 gccgggattc cttatggtgc gtttgtggcc gagcggttgg aaaagcctat gatttatgtg
    19381 cgcaaggagc ctaagggcta tgggcgcatg gcacagattg aagggcactt gccggataaa
    19441 ggtacgccaa aagtggtgct gattgaagat ttacaaaccg atggtggcag taaaaagatc
    19501 tttgttgacg cattgcgtga agtaggcgca caggttgaac atgcctttgt cgtttttcat
    19561 tacgggattt ttgctgccag cgaacaaaat atgaaagata tgggcattac gctgcacgcc
    19621 ttggcgacat ggtgggatgt gctggcaatg gccaaagaga aaaactattt tgacaaggaa
    19681 actctggcgt ccgttgatgc gtttttgaac gatccggaaa gctgggagcc tactcatcaa
    19741 gagggcgctg catgaaatta ggattttttg ccggaaaaaa accgcgcgcg caaaaagcgt
    19801 tagaagagtt gaaaagcgct tatgggtctg tgccgcccga agaagccgat acgattatcg
    19861 ttttgggggg cgatggcacg atgctgcgcg ccttgcatgc ctttgccgat tatcaggcgc
    19921 cgttgttcgg catgaatctg ggtacgctgg ggtttttgct caatgaaagc cgtacaggcg
    19981 gtctggaaga gcggctggaa aacgccaaac gttttaccat tcatccactg gtgatggaag
    20041 cgatcgaccg cgatggtcaa aagcataacg aggtggcgtt taatgaagtc tcgcttttgc
    20101 gtgaaactca caactcagcc aagatcaaag tgcatgttaa tgatgacgtg cggattaaag
    20161 agctggtttg tgacggggtg atggtttcta cggcagtggg cagcacggcc tataactcct
    20221 ctgcgggcgg gccgattttg ccgctgcaat ccaatattgt tgccatgacg cctatttccg
    20281 cattccggcc acggcgctgg ccgggtgcac tgctccacaa caagagcaaa attacgtttg
    20341 aaattctgca gtctattgaa cgcccggtca gtgcgacagc cgactccaaa gaggtacgcg
    20401 atgtgaaaag cgtcgacatt cacgaatcta aaaagatttc gaaaacgctg ctcttcgatc
    20461 ctgaaaaccc gctgcaagaa aggattttca aggaacagtt tttttcttaa gatttttgtt
    20521 cgtactctgg cgtttttata agacgtgaat aatgatgtac gctacagcgc ctataaacaa
    20581 aaagtacaaa cttccaaata caaactcgat cgccaagcca gcgtaaaatc tcttttggga
    20641 agcgtagttt ttttgtttaa aacgccgggt tctgtattca tcagaaatga tacatcgcaa
    20701 tggcgcggtg acggctgtaa aagcccaccc tattaacaaa aactccccta caacacctag
    20761 tagtgcatgc atgttatata ctccaagggt tttaaaaacg ttttttagtt aactctcttt
    20821 tgtcattccg accgagcgac agcgcgtgga ggaatcttat caaactgata gcggataaga
    20881 tccctcggct ttctgctttc cccggatcaa gtccggggtc cgcagaacga gacgctcggg
    20941 atgacatgga aaaaattaag ctttccgcgg tttacccggt ttgacgtcgc gcatacggcg
    21001 tttttcggcc agttcgtttt ctatcagttc gagtttttcg gcaagttgtt cattgatgct
    21061 gccgcctttg acgatgccgc cttcggggat cagaccgagc ctttgggcga tatattggta
    21121 gccctcaatc agatcaccga gatcaaaacg aaagcggtct ttgtccagct tttcacctgt
    21181 tttggcatcc cagagacggc aattgtcggg tgaaatctca tcggccagca cgatataaag
    21241 ctcgccatgc tcaccataga tgcggccaaa ttcaagttta aaatcaacca gccgcagacc
    21301 gatgccggag aacaggccat tgaggtaatc attgacgcgc caggccattg cgaccatttc
    21361 ttcaagctcg taaggatcgg cccaaccgaa ggtcatgatg tggtcttcgc tgaccatcgg
    21421 atcgcccagt tcgtctttct tgtagtaata ttcaaccagc gggcgttgca ggattgtgcc
    21481 ctcttccacg ccgaggcgct tacacaaaga cccggcggcg atattccgta caaccacctc
    21541 aagcgggata atttccactt cgcgcaccag ttgctcgcgc atattaatgg agcgcataaa
    21601 gtgcgtcggg ataccgatac cttcaagctt tgtcatcaga tgtgcggaaa tgcgattgtt
    21661 caatacgcct ttgcccgcga taacgtcatg ttttgcgccg tttcctgcag tcgcatcatc
    21721 cttaaagtat tgtataaccg tgcccggctc cggcccctca taaagcgttt tggccttgcc
    21781 atcataaatc atttttctgc gcggcatagc tcgctccttc tttgttccaa ggctgcatca
    21841 ttaaagcatt tacatgtata taacaatgga ttttaggcga ttccaatgct acaaaccatg
    21901 ttagaatgtt attttcagtg ataaacgcac attcaagagg tagaaatgtc atcatttgac
    21961 gaacggaaag acgcatttga aaataaattt gcgcatgaag aaaaactgga ttttgctgta
    22021 gaagcccgtt gttcgaagct ttttggcctt tgggtggccg agcaatttgg tcttgagggt
    22081 gcggatgcta agacatatgc gatggaggtt gttgaatcca atcttgaaga gcccggcttt
    22141 gatgatgttt tacgcaaggt aaaaggcgat ctcgaaggaa aagacattga aatatcggat
    22201 catatgctca acacacagct tgatattgcg cttgaaaaag cgaggaagca gattaaggag
    22261 gaaggcgcat gaaaagcgcc atcatcccat ttccgcattt aaacatcaaa aaagcctcga
    22321 aaatttcgag gcttttattt ttcccactca aacccaatct tgccgcactt ggttgcaata
    22381 agatcgggta atctccccat tcaacacgcc tagctttttg ccttactctt tacatgttgc
    22441 agtgtcttta tactctttcc aatttataga agcaataaaa aatatatttt aatagaatgg
    22501 tttagttaaa ttctataatt taaacactct tctgcatttt ttattcggtt cgcataaaaa
    22561 cgttttgcat ttgacttgtc attacgcttg aagcacctta catcttttga tgatacaagc
    22621 ccttacgtga aagaaaatac catggctatg gatgcagaag cgatcaagac catgatcacc
    22681 gccaatattc cggacgcaga agtgcgtatt gaagatttgc gcggtgatgg cgatcattat
    22741 gcggctcata tcacggcacc gtgttttgcg ggtaaaagcc ggatacagca gcaccagatg
    22801 gtttatgggg ccctaaaagg taaaatgggg ggcgatctcc atgcgctgtc gatcaaaacg
    22861 tgcgctccaa aagaaggtta acacttacaa ggaaagaatt ttaacgatgc aaaatgtttt
    22921 atttgaacgc attcaaaagg aactggatga tcatgatgtg gttttgttca tgaagggtga
    22981 agcggctttt ccacaatgcg gcttttcctc tctggtggtg caggttcttt cgcagctagg
    23041 cgtcccgttt aaagacatca acgtgctgga agacagcgat ctacgtcagg ggatcaagga
    23101 ttttaccaac tggccgacta ttccgcagct ctatgtgaag ggcgagttta tcggtgggtg
    23161 cgacattgtc cgcgagatgt atgaaagcgg cgaactacag catctattcg atgaaaaagg
    23221 cgttaaagcc gcctaacggc tcaccttcgt atcgggatgc agactgcagc tcatcatcag
    23281 gccgaaaccg atgagcgcag cgagcattga ggtacctcca tacgagatca gcggcaaggg
    23341 cgcgccgaca accgggatta accccatcac cattgcgata ttgataaaca catagagcga
    23401 gaaattcaca ctcaggccca gtgccagata acgtgcgaaa ttctggcgcg atttaagtgc
    23461 gatcaacaac ccgtagagca aaatcaaacc aaagagcata agcaggatta ctccaccaaa
    23521 gagcccctgc tcttccgccc aaagcgtgaa gataaaatcg gtttgttttt caggcaggaa
    23581 gttgaggcgg ctttgcgtgc cttccaagaa gcctttgccg tttatcccgc cggagccgag
    23641 cgcaattttc gactgggtga tgtgatagcc ggcgccaagc ggatcgctct ccgggttgag
    23701 gaaagtcagc acgcgttgtt tctggtaatc atgcataaaa tgccaagcta ccggaatact
    23761 tgccagcgcc aaaacgccac cgccgatata aagccagatt gaagcgccgg ccatgaatag
    23821 catggccgca ccaaccatca caatcatcag tgaagttccc aggtccggct gtaacagcac
    23881 cagtgcaaca ggcgcgagaa taatcagtgt cgggatcacc agaaacggca agcggcgcat
    23941 atcgtcaatc gtggccgcgt ggaagtagcg tgccagggcc ataaccacca cgattttcat
    24001 aagttcagat ggctgaagct gcataaaacc cagattaatc caacgctgcg cgcccatgcc
    24061 aacgtggccc ataatttcaa caataatcag cagtcccaac cccagaaaat acaaaggcca
    24121 tgtcaaacgg aaccaccatt tcaagtcgat catcgcaatg atcaccatcc ctattgctgc
    24181 gatgccaaag cgcatcatct gtttggatgc ccaaggtgaa aaattgcccc ccgcagcaga
    24241 atagagtgca gcgaaaccga tacaagatac cgctgtaatt aatagtataa gcccccaatt
    24301 gagatgcttg agtttttcga acatgttttg atcggtgtgg agtctgatgg aatggcgcat
    24361 aattatgctg gctccttcga tttgagccgc gatgcggtac cttgcgtctg cccgtgaaca
    24421 ggcgtggcgc caggattgcg cttttgcgtt tcaatcagca ggtctcgcgc caaaggcgcc
    24481 gccgtgcgcg agccaccaat gccatgttct accacgacag aggctatata acggggattt
    24541 tccagcggcg catagccgac aaacagcgca tgatgacggc ttttccattg tagttcttca
    24601 ttcttaatgc cggacatgcg ctgcttcatg gtaatgcgct gaacttgtcc ggtaccggtc
    24661 tttccaccaa aggccaatgc cggatcctct gcgcgtgaac cataggctgt gcccttttca
    24721 tcgttaacca cgcggtccat tccggttttg atcaaattca aatgctccgg gctaataccc
    24781 agttttggcc acccactttc aggcataata tttttcgcca gcccatcggc cgtgacccaa
    24841 ggtttaacgg cataaccgcc attaacgagg cgcgcggtca taacagcaag ctggagcggt
    24901 gtagcctgca aatacccctg accgatgctg gccacaacgg tttcacctgg ctgccatttt
    24961 tgaccgattt tgccgcgttt ccaagcctta tcaggcacca gcccggcgcg ttcttcggtg
    25021 agttcaaagt caagactttg ccccaaaccc atcatgcggg cgacatgcgc aattttgtct
    25081 atcccgactt ttgtcgacaa ttcgtaaaaa tatacatcgc aagacatttg cagcgcttta
    25141 cgacaatcaa cccaaccatg gcctccacgc ttccagcagt gaaagcgatc actcccgagt
    25201 tcgtaatggc ctttgcaaaa ggctgtggtg ttttctgtta cggcgcctgc ttgcagcccc
    25261 gccagcaggc tgatcatttt gaaggttgat gcgggcgggt attgccctga aacggccttg
    25321 ttggtcaacg gatgggcggg attagccagc aactcctccc aaaaggccgc cgaaatgcgc
    25381 gaggtaaaag cgttggggtc gaaagacggt gcggaggcca gcgcataaac tgcgccgcta
    25441 tgggcatcca tgaccacagc cgaggcgctt ttttcctgtg acagacgtcc ttgcataaaa
    25501 cgttgaagct cgccatcaat cgtgagcgtt atgcgcttac cgttgcggct ttgatttttt
    25561 ttaagctcgc gcacttcacg tccaacgacg ttcacttcga cttcagcctg accggcactc
    25621 cctcgcattt ccagatcaaa gcgcttttcg attccggttt taccgatttt aaagccagga
    25681 aggcttaaga gcggatcatc accgacatcg tttttgctga ctgcgccgac atagccgacc
    25741 aaatgggctg tagcctcctt aaacggatag gtccggcgtt tgcctgcatc ggtgcttaag
    25801 cctggcaaat ccggcaaatt gacctcaatt tttgcgacat ccttccaact caaatcatcg
    25861 gtgatttcaa tcggcacgaa ttttgcggtt ttcccggctt ctttaagaac ggcctgaata
    25921 tcgtcttcgg acaactcaat catcccttgc aagcgccgga gcgtttgctc gatattcgcg
    25981 gcctgctcgg gaataatcag cgccctgaaa ttctgggcat tgaccgccag aggcacacca
    26041 aaacggtcga caatttcacc gcgcacgggt gagagcactt tcaggttgat tctgttttta
    26101 tcggataagg ttttgtagcg agctccctgt gaaatctgca gccacgccag ccgcccgccg
    26161 agtacgccga gcagtgcgcc ctgcaatccg gcgatgacaa atgcgcggcg tgtaaaaacc
    26221 cgaacctgat cctgattttt tttcatgtgt ctaattataa ggtcatatcg gttttttgcg
    26281 accctattct ggttttactg cggctgattg gtgcgggcaa aatcttgtgt gaaagatgca
    26341 agaacagata cagcaccgga aagagtacta tacccagaac ataggatgag acgaaatcgc
    26401 gcacgggcat ccattgcagg cttagtaaac atgacatgct ccattgaaaa atatggaaga
    26461 ttgctgccaa tgcggcaaag cccatccata taaccatgaa ggactgaccg gtcagaaatc
    26521 gtctttgatc gacgacgact atgcgcccca gcaggaacaa aatggcactc aaacccaaat
    26581 atggcatgcc tgtgatgaga tcaagcaaca ggcctatagc aaaaacgagc cagggcggca
    26641 gcaatgtagg gcggtaaaca gaccagtaat aaattgccat caacaataat ggcgcctgaa
    26701 aaaaaacggc caatgggtag ggaacgctca caacactgag aatgaagatc atcgccagca
    26761 cggcgtaggg cacgagcatg cgcgcgatga actccatttt gtctgtcagg gattcataag
    26821 ggcccatatc aagtttgcct gctcaggttt tggacaataa aaaagagcct atcaggctct
    26881 cgacgatttt tcattgaaaa aggttttaaa aaagtcctgt caactctcac actaagccta
    26941 ttgcaaaagt aggataattt tgtaacaggt cctcatgatg cgagtgttaa ggacaactat
    27001 tttcgggttt ggtgaaaaca ggttggtagt cgtccgtgac atccatacac atgcaaggtg
    27061 tcaaactttt cccgctctgg gcaaagtaca tacaaggcgt gcttatattt tctatccaga
    27121 aaagtttttc accatcaggt gtaaaattaa atgcgtattt atgtgttttg tcttcgacaa
    27181 cagaggtggt ctcggttctt cggccagctt tgcggcgaag ttaagctacg atcg
//
LOCUS       DAOSWR010000007        26611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1160_length_26611_cov_5.261410, whole genome shotgun sequence.
ACCESSION   DAOSWR010000007 DAOSWR010000000
VERSION     DAOSWR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 26611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26611
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_26611_cov_5.261410"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..3993
                     /locus_tag="PK513_01175"
     CDS             199..3993
                     /locus_tag="PK513_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81099.1"
                     /translation="MRDKAAVKMTESPKVTKKEADEKAPVKSAQDLSIDVRASKAQEL
                     REKISDKTIGKASGFGALKISAVSEGHGDTLSERTESFDHDEEPVLLLKPSNQNISLG
                     ETPRTMKIAGAAGVFATLFWLGFCVAYIATAPALELTPQSLGAVLAGMMAPPALFWLI
                     LSSLNRRSDIDIYAASLRSELQTLLFPSEETASLINKDVERLCRQAAEIAAASKATLK
                     SLQRARQGLRVEIRDFSGVSKKAEFHIDRLAEGLQERSGKLLSLTEEIEQRTATIDER
                     TQVGADAWDQATLIVLERAAEMEAALGKGTDKLLEAAEKTSDKTKDIENALQGTYTAL
                     NDTVDQIAGRLEDLSGRFDDHRSGLADTADTMSNETERLAEMIRDQVEGLEEITDRTI
                     EAMTRSADTIKSHKQGLEDSAQDLALRSQDIAKTLSSEVRNIHEAIDFADEKSSKLEE
                     RLNSKTQKLHDVVSGIADEANVIEIAGEKAAHQLSEALSSAIAGSENIGSAVNRAVEA
                     LDIATQSANDKAEGLLQNTQNHIGSLNEAGRKHAEEIRNIAKMLEDSRAQIESASTAT
                     QSHIEDLSQAVEAQNHQITVSSASLMERISAVKEALSAPLSDIRSAINDADMRHEMIE
                     SVLKRRVSDLNEASEKATQSAENIREILRGQAQEISSLSGRISGNARTINDQMRTQKD
                     DLAAQVRDALEKVDSVRMALDKQSQDIEHISEQACEKISVFSKELSGRCVEMSNLTRH
                     NFDRLDELDEALAEKTGALKSRADEAAQSIEIFKTALLDTAEEIEPVYEKAIQGAEDV
                     QDRFETLRIEYEKTSESNLNKLRQIGVVFDSRLEDLKAGSDEAASILKSSSDHLQARA
                     QDIETASRSASSKMQEIAAALSDQSSDIHLTTDQALLKIETVQKAINEQFHELSASVG
                     QAIARIQDAGTEFTHQSEGLQAQSDRVAMHFEGLGAKAREETGMLHEASQKTVIDTEE
                     MVRRVQAEAEDLLSKAQGALLDLKKSGDSFTLRAREIEEHMKASLKTSEKYGQQMRTQ
                     AAQVAETSAQSVDKLGKSIKALKTRMDDIGQAANDVSAKVESTREKLSGESERLISVS
                     TAALESAQTASQTFGKQSETLLKASQDAVQAAEKMHQNTLHVQRENFMNSAKFIVESL
                     HSMSVDLTRIKDGEISEKTWKAFQKGDVSAFTRRLSGLTDQAAIDKARDKFAKDTEFR
                     TYVQRYIRQFEEMYTQAQTNDHGAMLSSTIASSEIGKLYETLVSIAGKKSAISEGTLK
                     AA"
     gene            4105..5037
                     /locus_tag="PK513_01180"
     CDS             4105..5037
                     /locus_tag="PK513_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HOO81100.1"
                     /translation="MNTKTKFCNGISDISDSYAGFIIDQWGVLHDGHKPYDGVVDCLK
                     ELKSRKKHIIILSNSGKRAEANKERLKKIGIGPSLYDQIVTSGEITWQGIDQQNEGFF
                     IGLGKKVYVISRGGDRSIVDGLDIEVVGDPAKADFLIISGSDAPDKTLEDYEPTLKSA
                     ARKGLTALCANPDSRGVMGSQSIMGPGTLARRYQDFGGVVHYIGKPHQPIFKHCIRIL
                     QEKEIYPGQTIVIGDALAHDILGGDLVNIDTCLVKTGLHTHAFEHAQTPASMNKALIL
                     LSHQFNNVRPTYLVESLKWGNPLPDRKHKKRAAR"
     gene            complement(5046..6026)
                     /locus_tag="PK513_01185"
     CDS             complement(5046..6026)
                     /locus_tag="PK513_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO81101.1"
                     /translation="MSDSESDKEVLDSEAPAHPPGIGLALGGGMARGFAHIGVLNVLN
                     RHGIRPSIVAGTSIGAVVGASYLAGKLDEFEDWALSLNRMRILSYLDFRVRSAGMIGG
                     HKLKKILEQNFKDMEIEDLPAPFIAIAADLVTGHEVWLRKGSLIEAMTASFALPGVFP
                     PVERNHRFLVDGALVNPVPVAPAQAMGARMTIGVDLNADIIGKAAKPGQGYQTVAGFD
                     LFNDADVPPEEQAKYTSTLTKRLFKRQEHAPSLFGVMVSALGIMQDRMTRSRLAGDPP
                     DIHIKPQIGDIGLLEFEKAEELIARGEDAAVKALPDIRDAMEVFLPPHDA"
     gene            6078..7853
                     /locus_tag="PK513_01190"
     CDS             6078..7853
                     /locus_tag="PK513_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO81102.1"
                     /translation="MSKDITHHAALPKKTTLNLMKRLVKTYLAPYWVSVLTALLFMAL
                     AAAMTAAVAQLMQPILDEVLNGKKTDMILPVAAVILTVFILRGISTYAHTIMMNKVGQ
                     SIVADIQKDLFSHFMVLDLQFFHDNPSGQLISRVINDVNVMRIAVSDTLTGFGKSILT
                     LVFLTGVMFYQDWKLSLVAFTIFPFAALFVARLGRRLRKVSTSIQKELGSLSDRLSQI
                     FHGIRQVQAYGMEDFERGRAGEAIDSVKNLNIKSVRIGNLSTPVNEILVGFVFFGIIL
                     YGGHESAEGRMSAGQLGAFIAAFTLAYEPMKKLARLNNTMQTGLGAAERVFQMIDLVP
                     NIQNESGARELADKTPSITFTDVQFQYEEGDTHALDGISFTAKSGSVTALVGPSGGGK
                     STVMNLILRFYDAKSGHIEIANQDIRTLTLETLRRHIALVSQDITIFDDTIAANIAYG
                     RPDATQDEIIAAARAAAAHDFIESFPRGYETQVGEDGVKLSGGQRQRLSIARAILRDA
                     PILLLDEATSALDNESEKLVQDALHKLEEGRTTLVIAHRLSTVQNADQILVLDHGRIV
                     ERGNHEALIQEKGLYAKMYQTGLKD"
     gene            7926..8390
                     /locus_tag="PK513_01195"
     CDS             7926..8390
                     /locus_tag="PK513_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO81103.1"
                     /translation="MPSHAAITEQFLALPPDTEVEKALKELKKKKLESAIIINGKGQV
                     EGTFSLQGVMKNLLPVSVAMEDGIQLDVTVRAAPGIAKRLRKVYPLKVSELMDRKVQA
                     VAPETPIWEVINYLVSHNDSLWVIEPESGKCLGMITAQSAMDELKRLQESES"
     gene            8387..8959
                     /locus_tag="PK513_01200"
     CDS             8387..8959
                     /locus_tag="PK513_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOO81104.1"
                     /translation="MKNLKPTAKYILILTALIGILAAFAMQPSKIHAQSQQPQETEPP
                     RTAIKIPPLLEAEPFAQDTLRIRKDNGEELYFNIELALTPRQQSQGLMYRTEMPKDSG
                     MLFLFNGPIKTSFWMKNTLIPLDMLFVHPDGTIHHIHHNARPQDKTSITSKFPAKAVL
                     ELNGGTADKMGIKEGDQIIHRLFNNDAAKP"
     gene            9012..9088
                     /locus_tag="PK513_01205"
     tRNA            9012..9088
                     /locus_tag="PK513_01205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9046..9048,aa:Pro,seq:tgg)
     gene            9115..9684
                     /locus_tag="PK513_01210"
     CDS             9115..9684
                     /locus_tag="PK513_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO81105.1"
                     /translation="MTTSPSNTNTKPTDEQRWREYAVKAQSGDKRAYNALLKEIASFA
                     RSYILSSLANPEWVDDITQEILISVHKSLHTYSADRAFRPWLMSIIHFRRTDYLRKYY
                     NARGNKQTTLDNADFQRANVTNPEHAGEYKDIEAALADLPENQRKVFEMIKIQGYTAK
                     EVAGEMGMSVSAVKVSAHRTAKKLKEALE"
     gene            9691..10332
                     /locus_tag="PK513_01215"
     CDS             9691..10332
                     /locus_tag="PK513_01215"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HOO81106.1"
                     /translation="MNEEKNIDTVINGLCDELEPCKCLAHPFVRVLPFLGVAIIYIAT
                     LACLIGIRSDLPQKFAEASFMFELFLMAFMSISAAISSSYLAVPGACEKKWIIIPPLT
                     AFGIFCMWSLILAVSEGMHMPVLHMDHCMGEGMFMALIPMTMMIVLVRNGATTFPKIT
                     ALMNALAATGLGYIGLRFTCSMDTVGHVTVSHLLPYVVIGMIMGLGARKLFKW"
     gene            10389..10691
                     /locus_tag="PK513_01220"
     CDS             10389..10691
                     /locus_tag="PK513_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HOO81107.1"
                     /translation="MKARIYKPSKTAMQSGRAKTKPWILEYESKSARCPEPLMGWTAC
                     EDTLGQVRLKFNTMEDAENYAESNGLYYIVQAEAVRKLKPRNYGDNFKYIPPEDED"
     gene            10766..10842
                     /locus_tag="PK513_01225"
     tRNA            10766..10842
                     /locus_tag="PK513_01225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10800..10802,aa:Arg,seq:tct)
     gene            complement(10879..12378)
                     /locus_tag="PK513_01230"
     CDS             complement(10879..12378)
                     /locus_tag="PK513_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81108.1"
                     /translation="MRGRFTHKRVSTLMEDIYGFEEFEAMKDAGVDRRMMFGINPHYQ
                     ALVTGEELCDNHGNVLIPKMPPSLPIAALILPRLNEMFSIEGAKDPSNQMNFTPGDND
                     LHGKLLHKYDEIVLGYASPTCSAHCRYCYRLDLFNKDTGKISVRPEELRDYILDYNRK
                     LEKNGGIDPKNGEKRYPIRELLLSGGDIMVLPNQTLYKFAAAAGQAGVSLLRFGTKEI
                     AFRPERFDEAMAETFRIIHAEFPHMHINIVSHFTHPDEFLVRDAENNYIPNTNGPGYQ
                     WMDISKEAVERILAHSFISVENQTPIILKVNDDERSLRVLHEELRRMGVKPKYIFQGR
                     DIEGHKAFSLPVEQAWKIHNNAMKGLSDTARSRFAMSTEWGKMEIVSIIDPLSNDAFN
                     FNFLPLAIQDTFKEILGDGLIVFKSHRSPHQARSQGDLIIAKRNPEALWISAYEDRII
                     YDTRKQDGQKYDGLISLLMGYFGENYIEQIQKRMPGASMTKALEAVSAA"
     gene            13186..13779
                     /locus_tag="PK513_01235"
     CDS             13186..13779
                     /locus_tag="PK513_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HOO81109.1"
                     /translation="MKHILSFLVVLLFPSSALAHEGVSGGFEAGISHPVLGLDHLLAM
                     VSVGIISSQIGGKAIWTVPTTFVIVMVVGGALGMIDMGLVAIEIGIALSVVVLGFTIA
                     GNAKLHQVGICAFVAMFAVFHGYAHGQEIPELVTSWSYIAGFMMGTAALHILGVAIGL
                     FAKRVRNGNVLLRYAGAVIAGMGIHILIGMAEFYYLF"
     gene            complement(13891..14421)
                     /locus_tag="PK513_01240"
     CDS             complement(13891..14421)
                     /locus_tag="PK513_01240"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOO81110.1"
                     /translation="MDILSEKILLRAITVLAVGSAQAAYAATGSVPIQAAVHGALAIT
                     QTRTLNFGSITDSGAGGTINVDNSDTPTVGGGVTSYGGTITSGGFTLRGTPGRQIDVT
                     APTSVVISNGAAATMTVGLFTINGAAGTINATAFTHILTVLTQTGFRLGGTLNVNPGQ
                     AAGTYTGTVNLTGNYN"
     gene            complement(15139..16521)
                     /locus_tag="PK513_01245"
     CDS             complement(15139..16521)
                     /locus_tag="PK513_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO81111.1"
                     /translation="MIQTKLQPFWPIIAMTALFLTAVFFRPLLPIDETRYMTVAWEMW
                     LRGDWFGPLTLNFEPYHHKPPLLFWLINDTWSIFGVSRWAGLLPVVFAATGCVYFTQI
                     FAQELFKAEKLEFKTLPFLMTGSVPFLIYSTLVMFDVTLTLFVLLALLALIAFANDRR
                     PVFILGMALAMGLGVLTKGPVAWLYVIFPVLFAPFWLDGHKKWLSWYGGCGLAFILST
                     IPVLLWLIPVLKASNPDFAFSLIWEQTAGRISGNMENAHTRPVYFYLPLLPVLFLPWA
                     FFPSFWTGLGSVKNSFKTNPALRFLACWIIPVVLGFSFIGGKQPHYLVPLLPGVLIFI
                     AYTMSMKPARIRAVSVAMVALIITGQIAASQTIFKKYDLQPIADYVSQHENKDCAFVR
                     KYQGELTFLGRLHHHMDDRTFENIEEWFHSHPNGIAVIRYHKPTEVNDFNMVMSIPYR
                     GKNIGIFEKP"
     gene            complement(16532..16849)
                     /locus_tag="PK513_01250"
     CDS             complement(16532..16849)
                     /locus_tag="PK513_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOO81112.1"
                     /translation="MTLTLPALNFETVWLAIGFLGQGLFFMRFFVQWLASEKAGKSVM
                     PDAFWYFSLAGGLVLFSYAIWRQDPVFMLGQSTGLFIYARNLYFIHNKEKDGTPEGVS
                     GSN"
     gene            complement(16863..17594)
                     /locus_tag="PK513_01255"
     CDS             complement(16863..17594)
                     /locus_tag="PK513_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO81113.1"
                     /translation="MISVVVPVYNEKDNIEALVREIAGAAENLPISEIVYVDDASTDG
                     SFDLLKQLRPQYSALRILKHKNQSGQSAAIWTGVRAAGNDLIVTMDGDGQNDPTDIKT
                     LWEAYEYHKKTLPKVIVLGERIERHDSLVRRLSSRIANSIRSGFLKDQTKDTGCSLKL
                     FQRKDYLNLPYFDHMHRFLPALMMRDNVQLVHVPVSHRPRVHGQSKYGTLDRALVGIS
                     DIRGVLWLQKRNRRANFDDVYEELN"
     gene            complement(17677..18288)
                     /locus_tag="PK513_01260"
     CDS             complement(17677..18288)
                     /locus_tag="PK513_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO81114.1"
                     /translation="MQRKHISLQLDILRRAKTDVEKEDAIAELYKGVERSAHLVKQLL
                     ELARQEPDAVQAPSKICDLRDIVTDVLEQHRALAKDKNIDIQFTPDENCNIEGDKNAL
                     GIMVGNLLNNAILYTETGGKVQVSLSKTPDGATLRIADNGPGIPEEEKTRIFDRFYRI
                     VGTNATGSGLGLSIVKTIAQRHNGTITVDKGLDDTGTAFNIVF"
     gene            18369..19415
                     /locus_tag="PK513_01265"
     CDS             18369..19415
                     /locus_tag="PK513_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO81115.1"
                     /translation="MKAVILARVSTKEQQEDGHSLDAQLGNLKNYAKLKNLTIIKQYT
                     VIESSTKGGRPEFMRMLDFVKAQKEKIAVIADTVDRFQRSFREMPLLLDLLDRDAAEY
                     HFIKENNVLTNNSNSMERAMWGIGVVMAQSYTDQLSDNVKRSVKHKVANGEWPGTAPL
                     GYLNAIDALTAKKTIIPDPDRGFLIKRFFEEYATGVYSLSELGRKAKEWGLRSRKGKE
                     VSNQTLHDLIQNPFYYGVMRIKGGLQPHRYESLISKELFDACEAVRTGRGRTQAVAET
                     KYPFIFRGLIKCAVSGRIVSCDRKKERHNYLICRKPKDADKKMWVKEEDVLEQVRDVF
                     LSCNRSASFCLRPS"
     gene            complement(19372..19503)
                     /locus_tag="PK513_01270"
                     /pseudo
     CDS             complement(19372..19503)
                     /locus_tag="PK513_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(19670..19744)
                     /locus_tag="PK513_01275"
     tRNA            complement(19670..19744)
                     /locus_tag="PK513_01275"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19710..19712),aa:Asn,seq:gtt)
     gene            complement(19852..20949)
                     /locus_tag="PK513_01280"
     CDS             complement(19852..20949)
                     /locus_tag="PK513_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81116.1"
                     /translation="MIKKSILFVFALFLLNISALYADESLAFTKISPQGDKALNGIFD
                     LSVEYGADGVGWMAYSRVSVPEFVSTHLAKSMDNGASWTYISTINKSSAEDVRVNGKS
                     FKGVWRYETPSLVYDPRDIPARRWKLFSQRYYVLPPFKKDDGLMREGWIEYKSAPNPE
                     GPWSKPECLFGHKKHNCRVQPNSLHPSLSGNLFYNEVGAFFYDGVLYMSLDANTAPSG
                     VGDWEKRKTVLFSSNDHGKSWRYNGVLTDYNDASGFGYFALTGTSLIEHDGQPYLLIT
                     PSGKKGLFVKNRAHDGCYLVAFEDISRAKLKRDSTGKLNVIKELSPEYNSGGLCDYHQ
                     KNSKGGVLFSQIDMKKMPEIFGIYNTRWIPR"
     gene            complement(20979..21052)
                     /locus_tag="PK513_01285"
     tRNA            complement(20979..21052)
                     /locus_tag="PK513_01285"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21018..21020),aa:Cys,seq:gca)
     gene            21256..21921
                     /locus_tag="PK513_01290"
     CDS             21256..21921
                     /locus_tag="PK513_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOO81117.1"
                     /translation="MADFQQVRKNMVNGQISINGVIIPEILEAFENTPRELFVPEKYK
                     NLACNDEDIPLGRGRFILEPSVHAKMIQALEPKADDVVLEIGGGTGYGAAILSSLVST
                     VVVTESEPECIAKAQKLWDSLGLCNIAVFEGPLAKGNPEHGPYDSIIIKGAVSSVPEQ
                     ILEQLNEGGQLICVIKAAEKNTGQVTLFKKLSGERQYSSYPLFDAATPYLQGFEPKET
                     FHF"
     gene            22052..22591
                     /locus_tag="PK513_01295"
     CDS             22052..22591
                     /locus_tag="PK513_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019961476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HOO81118.1"
                     /translation="MSEQETEEEPSIEEILTSIRQIISDDDEEAAAEEVVADEPAPEP
                     DPEEEVIELTDKIDDEPEPEPEPEPEEEPEPEEEIEEPEPIEVEMQDSVTPSEPVSRE
                     DDEDALLTHAAENAAFDAFSELAKKTAVEHGGITVEEIVRSELRPLLKDWLDKNLPPI
                     IERLVQEELERVAKRALEE"
     gene            22682..25333
                     /locus_tag="PK513_01300"
     CDS             22682..25333
                     /locus_tag="PK513_01300"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOO81119.1"
                     /translation="MLEKTFDPKAIEKNRYKRWKNSGAFACNPEGNAQPFTIMMPPPN
                     VTGSLHIGHALNHTLQDILIRYQRMKGRDALWQPGTDHAGIATQMVVERKLAEEGASP
                     RREMGRKKFLEKVWEWKAHSGGTIVDQLHSLGTTPDWNRERFTMDEGLSKAVLKAFVQ
                     LYHEELIYKDSRLVNWDPKLETAISDLEVEQKEQKGHMWHLKYPVEGEEEQFITVATT
                     RPETMLGDSAVAVHPNDERYKDIIGKSVVLPITERLIPIIADEYSDPEKGTGAVKITP
                     AHDFNDFEVGKRHDLPMINIFDRHACIAPLESIPSEYHGLDRYDARKKVLKEFEELDL
                     LVEVEDIKNAVPYGDRSGVVIEPFLTEQWFCDTPTLAKPAAQAVRDGRTRFVPQQYEN
                     MYFAWMDNQLPWCISRQLWWGHQIPAWYDEDGNVYVAETEEEAQAQAGTGVKLTRDED
                     VLDTWFSSALWPFSTLGWPDDGEEAKRMLKKYYPGDVLVTGFDIITFWVSRMMMMSLH
                     FMGEVPFKDVYVHALVRDAKGQKMSKSKGNVMDPLELSEKYGTDALRFTLAAMAAQGR
                     DIRLADERLQGYRNFATKLWNASRYCEMNGCKAIAGFDPAKVEYTPNQWIITALTEAK
                     VEIENALDAYRFNEAANAIYQFTWGTFCDWYLEFTKPVLQSGGDAAEEVKQTTGWVLE
                     QILILLNPFMPFITEELHEALTGRDQQIIAMNWPEYDERLGSPAAREKMDWLIRMISE
                     IRSVRADMNVPAGAKIQLLVKDACADTKAGLEIFGDIMKQMARLESIEHVEDVPKGAI
                     QTLVGETTLILPIADIIDLDAERMRLKKRIDKLEDDINKISQQLDNKKFIENAPEEII
                     AEQKELVAEKQSARKKLTAALQQLEAA"
     gene            complement(25417..25674)
                     /gene="rpmE"
                     /locus_tag="PK513_01305"
     CDS             complement(25417..25674)
                     /gene="rpmE"
                     /locus_tag="PK513_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOO81120.1"
                     /translation="MKADIHPDYHDIKVVMTDGSEYTTRSTYGKKGDVIKLDVDPLTH
                     PAWQGGTGKVIEKGQLSKFEKRFGNFGLAEDQKSEKANDKK"
     gene            25761..>26611
                     /gene="epmA"
                     /locus_tag="PK513_01310"
     CDS             25761..>26611
                     /gene="epmA"
                     /locus_tag="PK513_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOO81121.1"
                     /translation="MNWWLPHKFETKKTYLEVRVQLIRAMRAHFDDQDFWEVQTPILQ
                     TMPGADVHIHAYKLENGAYLHNSPEFAMKKLLVAGAPKIYQICPVFRREERTNLHNPE
                     FTMLEWYRAQADYTNIMKDCEELLRSLEIKEFHYNGQTCDPHAPWRRITVCEAFKQYT
                     DIDLASHFGNVDTFRSTVAEKGIRTIETDNWDDIFHAVMAEKIEPHLGQGAPTILYDY
                     PASMAVLSRKKPEDPRLAERFELYVCGVELANAFSELTDPAEQRARFIADMAEKQRLY
                     GESYPLD"
BASE COUNT         7550 a         6411 c         6137 g         6513 t
ORIGIN      
        1 tccacaatca ccgcttttaa aaaacacaac aataacaagc agataattta gtgcaaatcg
       61 ccgctctgtg aataactcaa acgttgtaaa aaaacccgct tgcgcccacc tgtctatcat
      121 ggctttttag aattccatcg aaaccatgga agaaatattg ctcgccacaa ttatggccgg
      181 caataaggag tgctgggcat gcgcgataaa gcggctgtaa aaatgacgga gtccccgaaa
      241 gtaacgaaga aagaggctga tgaaaaagcc ccggtaaagt ctgcacagga cttaagcata
      301 gatgtccgtg ccagcaaagc tcaggaattg cgggaaaaaa tttccgataa aacaataggc
      361 aaagcaagcg gcttcggtgc gctgaaaatc tccgccgtat ctgaagggca tggcgacaca
      421 ttatccgaaa gaacggaaag ttttgaccac gatgaggagc cggttctgct gctcaaaccc
      481 tccaaccaga atatcagcct tggtgaaacg ccgcgcacca tgaaaatcgc cggtgcggcg
      541 ggtgttttcg ccacattatt ctggctcggc ttttgcgtgg cttatatcgc caccgctccg
      601 gcattggaac ttacgccgca aagtctcggc gcagttctgg caggcatgat ggcaccgccg
      661 gcgttgtttt ggttaattct ctcatcactc aatcgtcgca gcgacatcga catctacgcc
      721 gcgtccctgc gctcagaatt gcaaacgctg ctcttcccat ccgaagaaac cgccagcctg
      781 atcaacaaag atgttgagcg tctttgccgt caggccgctg aaattgccgc cgcttccaag
      841 gcaactctaa aatccttgca gcgcgcccgt cagggcttgc gtgttgagat ccgggacttc
      901 tcaggcgtat ctaaaaaggc agaattccac atcgaccgtc tcgcggaagg cttgcaagaa
      961 cgctccggca agcttctgtc cctgaccgag gaaatcgaac agcgcacggc tacgattgat
     1021 gaacgtacgc aagtcggtgc cgacgcctgg gatcaggcca cgcttattgt ccttgagcgc
     1081 gctgctgaaa tggaggctgc tttgggcaaa ggcacagata aattgcttga agcagcagag
     1141 aaaacctcag ataaaaccaa ggacattgaa aacgcgctgc aaggcacgta tacggcgcta
     1201 aacgatacag tagatcaaat tgccggtcgt cttgaagatt taagcggacg ttttgacgat
     1261 caccgttctg gcctggccga tacagccgat accatgagca atgaaaccga acgccttgct
     1321 gaaatgatcc gcgatcaggt cgaagggctc gaagagataa ccgatcgtac cattgaggcc
     1381 atgacccgct cagccgacac gatcaaaagc cacaaacagg ggctcgaaga cagcgctcaa
     1441 gatcttgctc ttcgttctca ggatattgcc aaaacattat catctgaagt gaggaatatt
     1501 cacgaagcaa ttgattttgc agatgaaaaa agctctaaac tagaagaacg tctaaactca
     1561 aaaactcaaa aactccacga tgtcgtcagc ggtattgctg acgaagccaa tgtgatcgaa
     1621 atcgctggtg aaaaggctgc ccaccaatta agcgaggccc tatcctcagc cattgcaggt
     1681 tccgaaaata ttggcagtgc tgtaaaccgt gcagtagaag ctcttgatat tgcaacacaa
     1741 tctgccaacg ataaagccga aggcttgttg caaaatacac aaaaccatat cggtagtctc
     1801 aatgaggcgg ggcgcaagca tgccgaagaa attcgtaata ttgccaaaat gctcgaagat
     1861 agccgcgccc agatcgaaag tgcgtcaact gcaacacaaa gccatatcga agacctatct
     1921 caggccgtcg aggcccaaaa ccaccagatc acggtttcta gtgccagcct gatggagcgt
     1981 atcagcgctg tgaaagaggc gttaagtgcg ccgctttccg atattaggag cgcaattaat
     2041 gatgctgata tgcgtcatga aatgattgaa agcgtcttga agcgccgtgt ttccgactta
     2101 aacgaagcta gtgaaaaagc tacacaaagc gctgaaaata ttagagaaat tctgcgtggg
     2161 caagcacagg aaatttcctc gctctccggg cggatttccg gcaacgcccg taccatcaac
     2221 gaccagatgc gtacgcaaaa agacgatttg gccgcgcagg tgcgtgatgc tctggaaaaa
     2281 gttgatagcg tacgcatggc tctggataaa cagtctcagg atattgaaca catatcagaa
     2341 caggcttgtg aaaaaatttc agtattcagt aaagagcttt caggtcgttg tgttgaaatg
     2401 agcaatctga ccaggcacaa tttcgatcgc cttgatgagc ttgatgaggc tttggcagaa
     2461 aaaacaggcg cattgaaatc ccgtgctgac gaggcagcgc agtcaattga gatttttaaa
     2521 actgctctgc tggatacggc agaggaaatt gaaccggtct acgaaaaggc gattcagggc
     2581 gctgaagatg ttcaggatcg tttcgaaaca ctgcgaattg aatatgagaa aactagtgaa
     2641 agtaatttaa ataaactcag gcagatagga gttgtttttg atagcagact tgaagattta
     2701 aaagccggtt ctgacgaagc agcctccatc ctcaaatcct ccagcgacca tttgcaagcc
     2761 cgcgcccagg acattgaaac ggcgtctcgc tccgccagta gcaaaatgca ggaaatcgcc
     2821 gcagcattat ccgatcaatc ctccgatatc cacttaacaa ccgatcaggc gcttttgaaa
     2881 attgaaaccg tgcaaaaagc gattaacgag cagttccacg agctttctgc ttctgtcggg
     2941 caagccattg cccgcattca ggatgccgga actgagttta ctcaccaatc cgaaggtctg
     3001 caggcgcaat cagaccgtgt cgcaatgcat ttcgaaggtc ttggcgccaa ggcgcgtgaa
     3061 gaaacaggca tgcttcacga agccagtcag aaaaccgtga ttgataccga agaaatggtt
     3121 cgtcgcgtac aggccgaagc cgaagatctg ctgtccaaag cacaaggagc attgctggat
     3181 ctcaagaaat ccggcgacag ctttacactg cgcgcccgcg aaatcgagga gcatatgaaa
     3241 gcttccctca agacgtccga aaaatacggt cagcaaatgc gtacacaagc cgctcaggtt
     3301 gccgaaacct ccgcgcaatc cgttgacaag cttggcaaat ctataaaagc cttgaaaaca
     3361 cgcatggatg atattggaca agccgccaat gatgtgagcg ccaaggtcga aagcactcgc
     3421 gagaagctct ccggagagtc tgagcgcctc atcagtgttt caactgccgc actggaatcg
     3481 gcccaaactg cgtctcaaac ttttggtaag caatctgaaa cgctgctcaa ggcatcgcaa
     3541 gatgctgtac aggccgccga aaaaatgcac caaaacacgc tgcacgtaca acgcgaaaac
     3601 ttcatgaaca gcgccaaatt cattgttgaa agcctacatt ctatgtctgt cgatcttacg
     3661 cgaattaaag acggtgaaat ctccgaaaaa acctggaaag ctttccagaa aggcgatgtt
     3721 tcagccttta cgcgccgcct ctccggcctc acagatcagg ccgcgatcga caaagcgcgt
     3781 gataaattcg ccaaagacac ggagtttcgt acatacgtac aacgctatat tcgccagttt
     3841 gaggaaatgt acactcaggc gcaaaccaac gatcatggcg caatgttaag ctcgaccatc
     3901 gcgtccagcg aaattggcaa gttatacgaa acgctggtct ccatcgccgg taaaaaatcc
     3961 gctatctctg aagggacatt aaaggcggct taatgcgaat gagctgaata caattaaggt
     4021 ctattgatct ccgcttgcaa aaccataata atatgaagcg tcaaatttga gcattctctc
     4081 ttaagactat agacgggcca gataatgaac accaagacca agttttgtaa cggtatttcc
     4141 gatatttccg acagctatgc cggctttatc atcgaccagt ggggcgtact ccacgatgga
     4201 cacaagccct acgacggtgt ggttgactgc ttgaaagaac tcaaatcccg taaaaaacac
     4261 attatcatcc tgtccaattc cggcaagcgc gctgaagcta acaaagaacg tctaaaaaaa
     4321 atcggtatcg gtcccagcct ttatgatcag attgtaacct ccggggagat aacctggcag
     4381 ggaattgatc aacaaaatga agggtttttt atcgggcttg gcaaaaaagt ttatgtcata
     4441 agccgcggcg gagaccgctc aattgttgac gggcttgata ttgaggttgt gggcgaccct
     4501 gccaaggcag acttcctgat tatcagcggc tctgacgcgc cagataaaac gctcgaggat
     4561 tatgagccaa ccctgaagag tgcagcacgc aaaggcctga ctgctctttg tgcaaatcct
     4621 gacagccgcg gcgttatggg atcacaaagc attatggggc ctggcacact ggcccgccgt
     4681 tatcaggatt ttggcggcgt tgtacattat atcggcaagc ctcatcagcc aattttcaaa
     4741 cattgcatca ggatattgca ggaaaaagag atatatcccg gccagacaat cgtgatcggt
     4801 gatgcgcttg cccacgatat cctgggcggc gatctggtga atattgatac ctgcctggtc
     4861 aaaaccggct tgcacacaca cgccttcgag cacgcacaaa ctccggcttc aatgaataaa
     4921 gccctgatcc tgctttcaca tcagtttaac aacgtacgcc caacatattt ggtggaaagc
     4981 ctgaaatggg gcaacccgct tccggatcgg aaacacaaaa aacgcgccgc gcgttaagta
     5041 gaaggctaag catcatgcgg aggcagaaaa acctccatcg catctctaat atctggtaga
     5101 gccttcactg cggcatcttc accgcgcgca ataagttctt cggccttttc aaattccaac
     5161 aaaccaatgt caccgatctg gggcttgata tgaatatccg gcggatcccc ggccagccgc
     5221 gaacgggtca tacgatcctg cataatcccc aacgccgaaa ccataacgcc aaacagactc
     5281 ggcgcatgct cctgacgctt aaacagccgc ttggtcagtg ttgatgtata tttagcttgc
     5341 tcttctggcg gcacatcggc atcattaaac aaatcaaatc cggccactgt ctgataccct
     5401 tggccgggtt tagccgcttt cccgataata tcggcgttca aatcaacgcc aatcgtcata
     5461 cgtgcgccca tcgcttgcgc cggggctacc ggcacggggt taaccagcgc accatccact
     5521 aaaaagcgat gattgcgctc taccggagga aacacaccgg gcagcgcaaa cgaagcggtc
     5581 attgcctcaa tcaaagagcc cttgcgcagc cagacttcgt gacccgttac caaatccgcg
     5641 gcaatcgcaa taaacggggc agggaggtct tcaatctcca tatccttaaa attttgctca
     5701 agaatttttt tgagcttgtg tccgccgatc attccggcgc tgcgcacccg aaaatcgaga
     5761 taggataaaa tgcgcatccg gttaagcgaa agcgcccagt cttcaaactc gtcaagcttc
     5821 ccggccagat aactcgcccc gacaaccgca ccaatcgatg tgcccgcaac aatagacgga
     5881 cgaatgccgt gacgatttaa aacattcagc acaccgatat gcgcaaatcc gcgcgccata
     5941 ccgccgccta gggctaaacc aattcccggc ggatgtgccg gagcttcaga atccaaaacc
     6001 tctttatcgc tctcagaatc gctcatgtgt acttcttttc ttgaagtttt ctctccttca
     6061 agtataacac aaaacacatg tctaaagata ttactcatca cgctgcgctg ccgaagaaaa
     6121 caacgcttaa cctcatgaaa aggttggtga aaacatatct tgcgccatat tgggtgagtg
     6181 ttttgacggc cttgcttttc atggctctgg ccgccgcaat gaccgccgcc gtcgcccagc
     6241 ttatgcagcc aatcctggat gaggttctca atggcaaaaa aaccgacatg attttgcccg
     6301 tagccgcagt aattttgact gtgtttatcc tacggggaat ttcaacctat gcgcatacaa
     6361 tcatgatgaa caaggtcggc caatctattg ttgccgatat ccaaaaagat ctattttcgc
     6421 acttcatggt gctggattta caatttttcc acgacaatcc aagcggccag ctcatctcac
     6481 gtgtgatcaa tgatgtgaat gttatgcgca tcgccgtttc cgatacactc accggctttg
     6541 gcaaaagcat cctgacattg gtttttctca ccggcgtaat gttttatcag gactggaaac
     6601 tctcactggt cgccttcacg atctttccat ttgctgcgct ctttgtcgcg cgacttggtc
     6661 gccgcttgcg taaggtttca acatccattc aaaaagaact cggcagcctg tccgaccgtc
     6721 tctcacaaat cttccacggc atacgccagg tccaggccta cggtatggaa gactttgagc
     6781 gcggccgcgc cggagaagct atcgacagtg taaaaaacct gaacatcaaa tctgtacgca
     6841 ttggcaatct ttccacaccg gtcaatgaaa ttctcgtcgg tttcgttttc ttcggcatta
     6901 ttctttatgg tgggcatgaa tctgcagaag ggcgcatgag cgccggacaa ctcggcgcgt
     6961 tcatcgcggc ttttacactg gcctatgaac ccatgaaaaa actcgcaaga ctaaacaaca
     7021 caatgcaaac gggcctaggc gcagcggagc gtgtgttcca gatgatagat cttgttccca
     7081 acattcaaaa tgaatccggc gcgcgggaac tcgccgataa aacgccctct attaccttca
     7141 ccgatgtcca gttccaatat gaggagggtg atacccacgc tcttgatggc atcagcttta
     7201 ccgccaaatc cggcagcgtc acagcgcttg ttggtccatc tggcggcggg aaaagtaccg
     7261 tcatgaatct tatcctgcgc ttttatgatg caaaatctgg ccacatagaa attgccaatc
     7321 aggatattcg tacccttaca cttgaaaccc tgcggaggca tatagcgctt gtctcgcagg
     7381 acatcacgat ctttgatgat accatcgctg ctaatatcgc ctatggtcgc cccgacgcca
     7441 cacaagatga aattatcgcc gccgccaggg ccgccgccgc ccatgatttt attgaaagtt
     7501 ttccaagagg ctatgaaacg caagttggtg aagacggcgt aaaactttcc ggcggtcagc
     7561 gccagcgtct ttcaattgct cgcgcaatat tgcgtgatgc cccaatcttg ttgctcgatg
     7621 aagctacatc cgcgctcgat aacgaatctg aaaaactggt gcaagacgcg ctgcataaac
     7681 tggaggaggg acgtacaaca cttgtaatcg cgcatcgcct ctccaccgta caaaatgcag
     7741 atcaaatcct tgtgcttgat catggtcgaa ttgttgaacg tggaaatcac gaggccctga
     7801 tacaggaaaa aggactttat gctaaaatgt atcagacagg cttaaaggat taggctgaac
     7861 aggattgcat attttttcat tattaagcag aatagccata caaaatctat aaggactgaa
     7921 ctcagatgcc tagtcatgcc gccattaccg aacagtttct agccctccct cccgacactg
     7981 aggtagaaaa agcccttaaa gaactcaaaa agaaaaaact ggaatccgct attattatta
     8041 atggaaaagg gcaggtagaa ggtacgtttt ccttgcaagg tgtcatgaaa aatttattgc
     8101 cggtgtcagt ggctatggaa gacggtattc agcttgatgt aacggtgcgc gcagcacctg
     8161 gaattgccaa gcgcctgaga aaggtatatc cgcttaaagt ttcggaactc atggatcgca
     8221 aagtacaggc ggttgccccg gaaacgccga tttgggaggt tataaactat ctggtcagcc
     8281 ataacgactc attatgggtc attgagcctg aatcaggtaa gtgtttgggg atgattacag
     8341 cccaatcggc catggatgag ctcaaacgtt tgcaggaaag tgaatcatga aaaacttaaa
     8401 gcccaccgca aaatatatcc ttattctcac cgcattgatt ggaatcctcg ccgcattcgc
     8461 gatgcaacct agcaagatac acgcacaaag ccagcagcca caagaaacgg agcccccgcg
     8521 cactgcaatc aaaatccccc cactgctgga ggccgaacca ttcgcgcagg acactctgag
     8581 gatcagaaaa gataacggtg aagaacttta tttcaatatc gaactggctc ttacacctcg
     8641 ccaacagtct cagggattaa tgtaccgcac ggaaatgccg aaagattccg gaatgttgtt
     8701 tctcttcaac ggccccataa aaacatcctt ttggatgaaa aatacactca ttccgctcga
     8761 tatgttgttt gtgcatcctg atggcactat tcaccatata catcacaacg ctcgccctca
     8821 ggataaaaca agtataacat caaaatttcc ggccaaagct gttttggaac tgaacggcgg
     8881 caccgcagac aaaatgggca ttaaagaggg agatcagatc attcatcgtc tgtttaataa
     8941 tgacgctgca aaaccctaaa caagaacttg ctcttggaat tatgatagtg taagactgcc
     9001 cctcgaaggt acggagtgta gcgcagcctg gtagcgcaac tgctttggga gcagtgggtc
     9061 gggagttcga atctctccac tccgaccagc cttcccacat gaagtggagt attgatgaca
     9121 accagcccat caaacaccaa caccaaaccc acagacgaac aacgttggcg cgaatatgct
     9181 gtaaaggcgc aaagtggcga caagcgggct tacaatgcgc ttctcaaaga aatcgcctca
     9241 tttgcgcgca gctacatttt atccagccta gcaaatccgg aatgggtcga cgacattaca
     9301 caggaaattt tgatttccgt gcataaatcc ctgcatacat acagcgccga tcgcgcgttt
     9361 cgtccatggc tcatgtcaat tatccatttt cgccgcacag attacttacg caaatactat
     9421 aacgcacgcg gaaacaagca aaccacgctc gataacgcgg attttcaacg cgcaaatgta
     9481 acgaatccag agcatgcagg cgaatacaaa gacatagagg cggctttggc agatctgccg
     9541 gaaaaccaga gaaaggtctt tgagatgatc aaaatacaag gctatacggc caaagaagtg
     9601 gcgggtgaaa tgggcatgag cgtcagcgcg gtaaaagtct ctgcccacag aacagccaaa
     9661 aaactaaagg aagcattaga gtaactgcca atgaacgaag aaaaaaatat tgataccgtc
     9721 attaacggcc tttgcgacga gttggagcct tgtaaatgcc ttgctcatcc gttcgtcaga
     9781 gttttaccgt ttttgggtgt tgcaattatt tacatcgcaa cactggcctg cttaattggc
     9841 attcgttctg atttgccaca aaaattcgcc gaggcttcct ttatgtttga gttgttttta
     9901 atggccttca tgtcaatctc agcggcaatt tcctcatctt atctggctgt gccaggtgct
     9961 tgcgaaaaga aatggatcat catccctcca ctaacggcct ttggcatttt ttgcatgtgg
    10021 agcctcattc tcgccgtatc ggaaggcatg cacatgccgg tgctgcatat ggatcattgc
    10081 atgggcgagg ggatgtttat ggcccttatt cccatgacca tgatgatcgt tctggtgcga
    10141 aacggcgcaa cgacttttcc caagataacg gcgcttatga atgccttggc cgccacaggg
    10201 ctcggctata ttggtttacg ctttacatgc tctatggata cagtcggcca cgtcacggtt
    10261 tcccatcttc ttccttacgt ggttataggc atgatcatgg gactaggcgc cagaaagctc
    10321 tttaaatggt agaaatgacg gcgtatttgc tctaaattca aagaagaaaa attcaaggac
    10381 aaaccatgat taaagccaga atttacaaac cgtctaaaac agctatgcaa tcagggcggg
    10441 ccaaaacaaa gccctggatc ttggaatatg aaagtaaatc tgctcgttgc cctgaaccat
    10501 taatgggctg gacagcttgc gaagatacgc tcggccaggt tcgtctaaaa tttaatacaa
    10561 tggaagatgc tgaaaattat gccgaatcca acggcttgta ctacatagtt caggctgagg
    10621 ccgtacgaaa actcaagccc cggaattacg gagataattt caaatatatc ccgccggaag
    10681 acgaagatta gaaggccgag cagttaattc ttattttaca aagtcgaaat attctattat
    10741 tccattctat gatccagcaa aagacggtcc cgtagctcaa ttggatagag cacctgcctt
    10801 ctaagcaggg ggttccaggt tcgagtcctg gcgggatcgc catttcataa aaataggccc
    10861 aacaggatct ttttaggctt atgccgcgct cacagcctca agggctttag tcatagacgc
    10921 acccggcatg cgcttttgta tctgctcgat ataattttcg ccgaaatacc ccatcaatag
    10981 cgagataagc ccgtcatact tctgaccgtc ctgttttctc gtgtcataaa ttatgcggtc
    11041 ctcatacgcc gaaatccaca aagcttccgg gtttcttttg gcgataataa gatcaccttg
    11101 tgaacgcgcc tgatgcggcg atctgtgcga cttaaaaaca atcagcccat cacccagaat
    11161 ttctttaaac gtgtcctgaa tcgccaaagg cagaaagttg aaattaaacg catcgttgct
    11221 taacgggtca ataatactca cgatttccat ctttccccat tccgtagaca tcgcaaaacg
    11281 agagcgcgcc gtatcagaca gccccttcat cgcattattg tgaattttcc acgcttgctc
    11341 tacggggaga gaaaacgcct tatgtccctc aatatcacgg ccctgaaata tatatttggg
    11401 ctttacgccc atacgccgaa gctcttcatg caaaacgcga agagagcgtt catcgtcatt
    11461 cactttcaaa ataatcggcg tttggttttc aacggaaata aaactatgcg caagtatccg
    11521 ctcaacggcc tctttcgaaa tatccatcca ttgatagccc ggtccgtttg tattcggaat
    11581 gtagttattc tccgcgtcac gaaccaaaaa ctcatccggg tgcgtaaagt gtgaaacgat
    11641 attgatatgc atatgcggga attccgcgtg gataatgcgg aaagtctccg ccatcgcctc
    11701 atcaaaacgc tcaggacgaa aggcaatctc tttcgtcccg aaacgcaaaa ggcttacgcc
    11761 ggcttgtccg gcggcggcgg caaatttata taaggtctga ttgggcaaaa ccataatatc
    11821 gccgccggaa agcagaagct cacgaatggg gtagcgtttc tctccgtttt tcggatcaat
    11881 cccgccattc ttctccagct ttcgattgta atctaaaata taatcgcgta gctcttccgg
    11941 acgaacactg atcttgccgg tgtccttgtt aaatagatcc agccggtagc aatagcggca
    12001 atgagcagag catgtcggcg aagcatagcc cagaacaatc tcgtcatatt tgtgcaagag
    12061 ttttccatga agatcgttat cgcccggcgt aaaattcatt tggttagaag gatcttttgc
    12121 gccctcaata ctaaacatct cgttaaggcg aggcagaata agcgcggcaa tcggcaagct
    12181 tggcggcatt ttaggaataa gaacattgcc atgattatcg caaagctctt cgccggtaac
    12241 caaagcttga taatgcggat tgataccaaa catcatgcgc ctgtctacgc ctgcgtcttt
    12301 catagcctca aattcttcga agccatagat gtcttccatc aacgtactga cgcgcttatg
    12361 cgtgaatctg ccgcgcatgg gacgtatata tcgcaaatcc gatttaggaa cattctcgtt
    12421 cctttgaata acattatttt cgctagccat gttcaccgtt ctcgtttgca aaattatttc
    12481 gtaatgcata tcgacaagaa gtaaggttag tggaaaagag tgttggaagc tattgttttt
    12541 taagggtttt ttgtgtgcat cgcagcaatc ttgccgatat tgtgcaatgg tgcaatgcga
    12601 aaaataacgc cggaattttt gctaagttat tgtataagaa tttatatttg gtgacgctaa
    12661 agtgaaaaga atggccgtac caaaaagtgc acaaatgtgt acaaaaaggt cataaaaaga
    12721 tggctcaaag ggccaaaata aggaccgcgg atacacaaaa catacgaatc acacaaaaaa
    12781 ggtccggaga ccttttaaag atgctccgga caaacggcgg taatagaggg tgtgtagcct
    12841 ccaatatacg cgttttcgaa acgattgcaa ggaaaaaaca caaaatttat ataaaatttt
    12901 atcaaaaccg aaactaatag caattttaaa tataaagagt aagtaatcaa aaaataattt
    12961 agaattatac aagtataatt aattatattc tattattaat attatgcttt atataaaata
    13021 ttattgttaa tttattttat ttcaaaaata tatttcttgt ttttatatat tccatactta
    13081 tatttgtata atgttatagc tcacaaaaaa gcaattctat gagcgttgtc tttggtatga
    13141 ataccattta tcgtgattta gaattaaacc ctaaagaaac tttatatgaa acatatcctt
    13201 tcatttttag tcgtattgtt atttccttca tcggctcttg cacatgaagg tgtatccggc
    13261 gggtttgagg ctggaatatc acaccctgtt ttgggtctcg atcaccttct tgccatggta
    13321 agcgtcggta tcatcagctc tcaaataggc ggtaaggcta tatggactgt acccacaaca
    13381 tttgtcattg ttatggtagt tggtggcgca cttggtatga ttgatatggg ccttgttgca
    13441 attgagatcg gtattgcttt atcagtcgta gtcttaggat ttacaatcgc cggtaatgcc
    13501 aaacttcacc aggttggtat ttgtgcattt gtggccatgt ttgctgtttt tcatggctat
    13561 gcccatggtc aggaaatccc cgagctggta acttcatggt cttatatagc aggctttatg
    13621 atgggtaccg cagcgctaca tattcttggt gttgctatcg ggctttttgc gaagagagta
    13681 agaaatggaa acgttttgtt acgttatgct ggagctgtta ttgccggtat gggaattcat
    13741 atccttatag gtatggccga attctattac cttttttagt tcctgttggt tttggctttg
    13801 gagtagggtg tatatattgc cagatcggaa gtcttgaaag cagtggatca agatatagga
    13861 acccctcgac ggagcatgtt acggggcatt ctaattataa tttcctgtaa gatttaccgt
    13921 cccggtataa gtacctgctg cttggccggg attaacattt aatgtgcccc ccagcctaaa
    13981 accggtttgg gttaatactg ttaagatatg agtaaaagct gtggcattga tggtgccagc
    14041 cgcgccgtta atcgtaaaca agcctacggt cattgtggca gcagccccgt ttgaaattac
    14101 aacgctagtc ggtgccgtaa catcaatctg gcgccccgga gttcctctga gggtaaagcc
    14161 tcccgacgta attgtgccac cgtaagacgt cacccctccg ccaactgttg gggtatcaga
    14221 attatctaca ttaatcgtac cgcccgcacc ggaatcggtt attgagccaa aattcaacgt
    14281 gcgcgtttgt gtaatcgcaa gagcaccatg gacagcagcc tgaataggaa cgctgcctgt
    14341 agccgcgtac gccgcctgag cgctccctac ggccagaaca gttatcgctc ttagtaaaat
    14401 tttttcggat aaaatatcca ttatctatcc acacatgctc ttaatttcac atcctgacct
    14461 aacataatta aggcgcccct taataatatc cggatgcaaa tgcaatccta acatataaca
    14521 gacctgttgc aaaagtagtt tgattttgag cagggagaaa tctgggcttt gtgtttttct
    14581 atcagaaccc tgcgagaaga ttcgtaaagc ctgtttacct cctttgcaaa ggcagtttat
    14641 aatgtgaact gcaaagcctc tcactgttct gcataatata aaaactgaac ggcttttaaa
    14701 tccgaaagcg gctaatgaca aaggggataa tcctcgtcct gcccgcaata agaggcgcaa
    14761 gcctaatgga cagaataagc cccataatca atcaagaccc ggcaacaaga aacggtttta
    14821 tcgcagcaag aaaaaagcgg cttagaaatt tcagtattgt ttacggataa atcagttcgg
    14881 cttcacaaca cggacacgaa ccagcgacca aaatcttaat gtgtttgatg acgaaaaatt
    14941 gggacaaaat tgctgtggct tttcgccgcc tattgtccca ccagtgcccc aaatttgatt
    15001 ataatgtctg gaaagtggct cacccgtttt gatacattac gaacgcatca ttatcaagat
    15061 gtgctgttca tttacaagac aataagcttt acctgttatt tcgggaaaat gcaatgcggg
    15121 gcttacttga agccgaagtt acggtttttc aaaaataccg atattcttgc ccctgtaagg
    15181 gatggacatg accatattaa agtcattaac ttcagtgggt ttgtgatagc ggatgacggc
    15241 tataccgttc gggtgggaat ggaaccattc ttcaatattt tcaaaggttc tatcatccat
    15301 atggtgatgt aatcgcccta agaacgttag ttcgccctga tatttgcgaa caaaggcaca
    15361 atccttgttt tcatgctggc taacataatc cgcgatgggc tgtaagtcat atttcttgaa
    15421 aatcgtttgt gacgcggcaa tctgaccggt aatgataagt gcgaccatgg caaccgatac
    15481 agccctaatt cgggcaggtt tcatagacat cgtataggcg atgaaaatca atacacccgg
    15541 caacaatggc acaagatagt gtggctgttt tccgccgata aaggaaaacc caagaaccac
    15601 gggtataatc cagcacgcca aaaatcttaa tgccggattt gttttaaagc tattcttaac
    15661 agaccctagc cctgtccaaa aagacgggaa aaaagcccac ggtaaaaaca gaaccggaag
    15721 caacggtaaa tagaaataaa ccggtcttgt atgcgcgttt tccatgtttc cgctaattcg
    15781 gcccgccgtc tgttcccaaa tcagcgaaaa ggcaaaatcg ggattggatg cttttaaaac
    15841 cgggataagc caaagcagaa cgggtattgt ggaaaggatg aaagcaaggc cgcagccccc
    15901 ataccatgac agccattttt tatgaccgtc caaccaaaag ggcgcaaaca gaactggaaa
    15961 aatcacgtaa agccatgcga ccgggccttt ggtcaaaacg cccaaaccca tcgccaaggc
    16021 catccccaat ataaaaaccg gccttctgtc gtttgcgaag gcaataaggg caagcaaagc
    16081 cagcaagaca aacagggtca aggtcacgtc aaacatgacc aatgtgctgt atatcagaaa
    16141 ggggacactg cctgtcatca agaatggtaa ggttttaaat tcaagctttt ctgctttaaa
    16201 aagttcttgt gcaaaaattt gcgtaaaata gacacagccc gtggcggcga aaactactgg
    16261 cagcagtccg gcccaccggc ttacgccgaa aattgaccat gtgtcattaa taagccagaa
    16321 caataaaggc ggcttatgat ggtagggttc aaaattcagc gttaacggtc caaaccaatc
    16381 accccgcagc cacatttccc atgctaccgt catgtaacgg gtttcatcaa tcggcaaaag
    16441 cggcctgaag aacacagccg ttaaaaacag ggctgtcatg gcgatgatgg gccagaatgg
    16501 ttgcagcttg gtttgtatca tgtcaggtgc tttaatttga gcccgaaaca ccttcaggag
    16561 tgccatcttt ttctttgtta tggatgaaat aaagattgcg ggcatatata aaaagccctg
    16621 tgctttggcc tagcataaat accgggtctt gccgccagat tgcataggaa aataaaacca
    16681 gaccgcccgc aaggctgaaa taccaaaacg cgtcgggcat aacgcttttt cctgcttttt
    16741 cagaagccag ccattgcacg aaaaaacgca taaaaaaaag accttgaccc aaaaagccga
    16801 tggcaagcca gacggtttca aagttgagtg ctggaagtgt caatgtcata gtgtttcctt
    16861 ttttaattaa gttcttcgta aacatcgtca aaattagcac ggcggtttct tttttgaagc
    16921 cacagaaccc cccttatatc acttatcccg acaagggcac ggtccagcgt tccatatttg
    16981 gattggccgt gaacacgggg gcggtgcgat accggcacat gaacaagctg tacattatca
    17041 cgcatcatca gggctggcaa aaagcggtgc atatggtcga aatagggaag atttaagtaa
    17101 tccttgcgct ggaataattt taacgaacat ccggtatctt tcgtttggtc ctttagaaag
    17161 ccggaacgga tgctgttcgc aattcgtgat gacaggcggc gtacaaggct atcgtggcgc
    17221 tcgattcgtt caccaagcac tataactttc ggcaaggtct ttttatggta ttcgtacgct
    17281 tcccacagcg tttttatatc ggtgggatcg ttctggccgt ccccgtccat ggttacgata
    17341 aggtcgtttc ctgcggccct tacacccgtc caaatggctg cggattggcc cgactggttt
    17401 ttatgtttta gtattcttaa ggcggaatat tgggggcgaa gctgcttcaa aaggtcaaag
    17461 gaaccgtctg tgctggcatc atcgacatac acgatttcgc tgataggaag attttctgcg
    17521 gccccggcaa tttccctgac caaagcttcg atattgtctt tttcattata gacaggaacg
    17581 acaacactta tcatggatac aattcaccct tacttatttc gtgaattgca aaatgtaatt
    17641 tgaaacttaa gggaaactta agggcttaaa aatcattcaa aaaacgatgt taaaagctgt
    17701 ccctgtgtcg tccagacctt tatcgacagt gatagtgccg ttatgacgct gtgcaatggt
    17761 tttaacaatt gaaaggccaa gcccggaacc ggtcgcgttt gtgccgacaa tacggtaaaa
    17821 gcggtcgaaa atgcgcgttt tttcttcttc tggaataccg gggccattat cagcaatgcg
    17881 aagggttgca ccatccggcg tttttgataa agatacctgt accttaccgc cggtttccgt
    17941 gtaaagaatg gcgttgttca aaagattgcc gaccataatg ccaagagcat ttttgtcacc
    18001 ttctatgttg cagttttcat ctggcgtaaa ttgaatgtct atattcttgt cttttgcaag
    18061 cgccctgtgt tgttccagaa catccgtgac aatatccctt aaatcacaga ttttagacgg
    18121 cgcttgaacc gcatcgggtt cctgacgggc cagttccaac aactgcttaa ccaaatgtgc
    18181 gctgcgttcg acccctttat aaagttcggc aatcgcgtct tctttttcta cgtccgtctt
    18241 cgcgcggcgt aatatatcca gttgaaggga tatgtgtttt ctttgcactt caaagctttg
    18301 gcttttaaaa attcgccaaa cccattacaa ttttgctgtc agcaatccta aagtgaaaag
    18361 atttaagtat gaaagccgta atcttagccc gtgtatcgac caaggaacaa caagaagacg
    18421 gccattcgct ggacgcgcag cttggaaatt taaagaacta cgccaaatta aaaaatctga
    18481 ccatcatcaa gcaatatacc gtcattgaaa gttcgacgaa gggcgggcgg cctgaattta
    18541 tgcggatgct tgatttcgtc aaagcgcaga aagaaaagat tgccgttatt gctgacaccg
    18601 ttgacaggtt ccagcgtagc tttcgtgaaa tgccactttt gttggattta ctggataggg
    18661 atgccgccga atatcatttc atcaaagaaa ataacgtcct gactaataat tcaaacagca
    18721 tggaaagggc tatgtggggg attggcgtgg tcatggcgca atcctatacg gaccagcttt
    18781 ccgataacgt caagcgcagc gtgaagcaca aagttgccaa tggggaatgg cccggaaccg
    18841 cgcctttggg ctacttgaac gcgattgacg cactgacagc caaaaagacc attattcctg
    18901 accctgatag ggggtttctg ataaagcggt ttttcgaaga atacgcaact ggcgtttatt
    18961 ccttgtcaga attggggcgt aaagccaagg aatggggctt acgttcgcgg aagggcaagg
    19021 aagtcagtaa ccagaccctg catgacctta tccaaaaccc gttttattac ggtgtgatgc
    19081 ggattaaggg cgggttgcag ccccacagat acgaaagcct tatcagcaag gaactattcg
    19141 atgcctgtga agccgttaga accggcaggg gacggacaca ggctgtcgct gaaacgaaat
    19201 acccgttcat ctttcgcggc cttatcaaat gcgctgtatc aggccggatt gtcagttgcg
    19261 accggaagaa ggaacggcat aattacctga tttgccggaa acccaaagat gccgataaaa
    19321 agatgtgggt aaaagaagaa gacgttctgg aacaggtgcg cgatgttttc ttatcttgta
    19381 atcgtagcgc cagcttttgc ctccggcctt cataacaagc aggtatagcc cttggccatc
    19441 gcggagtttg taattctttt ccgtgggctt tgctgtgcgg catgcaatgt ctgtcagttt
    19501 cattttcggg tatctccttt tccgcatata cccgaatacc cgaagatgta cccgaaaaca
    19561 tgctggattt cgtcaaggtt attgagacgt tataggagcc taaaggcttc gataacgcta
    19621 ggtttcctag actttttgga ttgtatcgga ttttctggga taaggaaagt ggctcctcgg
    19681 gccggattcg aaccagcgac caagtgatta acagtcacct gctctaccgc tgagctaccg
    19741 aggaacacga ggcaatttta tgcctatagg aaaaagcttt gtcaacaata atggcacggt
    19801 tttctattaa gaagcgaagg atactttcct gaccgttgtt gattgttatt tctagcgggg
    19861 aatccagcgc gtgttgtaaa tgccaaaaat ttcaggcatc tttttcatgt cgatctgaga
    19921 gaacaaaaca ccgcccttgc tgttcttttg gtggtaatca caaagcccgc cagagttata
    19981 ttcaggggat agctctttta taacattaag tttgcctgtg gaatcacgct taagttttgc
    20041 acggcttatg tcttcaaacg caactaaata gcacccgtca tgggctctgt tcttaacaaa
    20101 caaccctttt ttgccggaag gtgtaatcag aagatatggt tggccgtcat gttcaataag
    20161 acttgtcccg gtaagggcaa aatacccaaa tccggaagca tcattataat ctgtaagaac
    20221 accattatag cgccagcttt ttccatgatc atttgatgaa aacagcacgg tcttgcgttt
    20281 ttcccaatcg ccgactcctg aaggggccgt gttcgcatct aggctcatat acaagacgcc
    20341 gtcataaaag aaggctccaa cctcgttata gaacaggttt ccagacaagg atgggtgcaa
    20401 cgaattgggc tgaactctgc aattatgttt cttatgccca aataagcatt ccggttttga
    20461 ccagggccct tcggggttag gggcactctt atactcaatc cagccttctc tcatcaagcc
    20521 atcgtctttt ttaaatggag gcagaacata atatctttgc gaaaacaatt tccatcgtct
    20581 cgccggaatg tctcttgggt cgtaaacaag actcggcgtt tcataacgcc aaacgccctt
    20641 aaagcttttg ccattaacgc gcacatcttc ggcgctggat ttatttatcg tggaaatata
    20701 tgtccaggac gcaccgttat ccatactttt agcaagatgc gttgaaacaa attccggcac
    20761 agaaacccgg gaataggcca tccaccctac accatctgca ccatattcca cggaaagatc
    20821 gaatatgccg ttaagtgcct tatctccttg cggtgaaatt tttgtaaacg caagagattc
    20881 atccgcataa agggcagaaa tgttaagtaa gaaaagagca aaaacaaaaa gaattgattt
    20941 cttgatcatg gtctaacatc tccttcatta ggggagaatg gaggcacgga ccggagtcga
    21001 accggtgtac acggatttgc aatccgctgc atagccactc tgccaccgcg ccaacaataa
    21061 caaacagata caaataaatg caaactgtta tgatgtttag tgtaaaatac ctatgcaagt
    21121 caattgtgaa agtcttgtac ggagtgtttt atgtaacgaa tcagaggcaa tgtttaaaaa
    21181 cggctggaat gatcatgata actacggtaa acagaagcta acagattgtt tcacaacatc
    21241 aagagcgcac aagtcatggc ggattttcag caagtacgaa aaaatatggt caatgggcaa
    21301 atcagcatca acggcgtgat tattccagaa attcttgagg cttttgaaaa taccccgcgg
    21361 gaactgtttg ttcctgaaaa atataagaac ctggcctgta atgatgaaga tattccatta
    21421 ggacgcgggc gctttatttt agagccgtct gtgcacgcca aaatgattca ggcgttggag
    21481 cctaaagccg atgatgtcgt attggaaatt ggcggcggga ccggttatgg cgcagcgatt
    21541 ctctcatcac ttgtctccac ggtcgttgtc actgaaagcg agcctgagtg tatagccaaa
    21601 gcccaaaaac tttgggattc tttaggatta tgcaatatag cggttttcga agggccgctg
    21661 gcaaaaggca acccggaaca tggcccatac gattcgatca ttatcaaggg cgccgtatca
    21721 agcgttccag agcagatact ggagcaatta aacgaaggtg gacaactgat atgtgttata
    21781 aaggccgcag aaaaaaacac cggacaagta acattattta aaaaactgag cggagagaga
    21841 caatattcat cctatcctct tttcgatgcg gcaaccccct atttgcaagg ctttgagcca
    21901 aaagagacct tccattttta actagtcttt gtgtagtaaa taaaactcag atgtggatag
    21961 gctgagagaa actctgctct ccctctttga caagctgtgt agtatgatag aaactgaaat
    22021 gaaataacac gctgatatat attcaatttg catgtctgaa caagaaaccg aagaagaacc
    22081 gtccatcgaa gaaatcctga cgtctatacg ccagatcatt tctgacgacg atgaagaagc
    22141 cgctgctgaa gaagttgttg cggatgaacc tgctccagaa ccggatccag aagaggaagt
    22201 tattgaatta acagacaaaa tcgacgatga gccggagcca gaaccggagc cagaaccgga
    22261 agaggagcca gaaccggaag aggagatcga ggaacctgaa ccaatcgagg ttgagatgca
    22321 ggacagtgtc actccgtctg aacctgtttc aagggaagac gatgaagatg cgctgttgac
    22381 ccacgccgcc gaaaatgctg cattcgatgc tttttcagaa ctcgcgaaaa aaacagccgt
    22441 agagcacggt ggaatcaccg ttgaagaaat cgttcgctcg gagctgcggc ctctgctgaa
    22501 agactggctg gataaaaact tgccgccgat tattgaacgc cttgtccagg aagagcttga
    22561 gcgcgtggcc aagagggccc tcgaagagta atcccttatc ttttttgttc ctaaaacctt
    22621 gcttttgcaa ggaaaaccct tatgatctgt gcgctaaaca agatcgctca aaggataagc
    22681 tatgcttgaa aaaacatttg atcccaaagc cattgagaaa aatcgttata agcgctggaa
    22741 aaattctggt gcattcgcat gtaatccgga aggcaacgcg caacctttca ccatcatgat
    22801 gccaccgccc aatgtaacgg gatctttgca tattggccat gcgctaaacc acacactgca
    22861 ggatatcctg attcgctatc aacgcatgaa aggccgtgat gctttgtggc agcccggaac
    22921 agatcacgcc ggaatcgcta cgcaaatggt ggtcgagcgt aaactggcgg aagagggtgc
    22981 ctcaccacgc cgtgaaatgg gccgcaaaaa atttcttgaa aaagtctggg aatggaaggc
    23041 tcatagcggc ggcaccattg tcgatcaact ccactcactg ggcaccacgc ccgattggaa
    23101 ccgtgagcgg ttcacgatgg atgaagggct gtccaaagca gttctcaaag cgttcgttca
    23161 gctttaccac gaggagctga tctacaaaga cagccgcctt gtgaattggg accccaaact
    23221 cgaaacggcc atttccgatc ttgaggttga gcaaaaagaa caaaaaggcc atatgtggca
    23281 ccttaaatac ccggttgaag gtgaagaaga gcaattcatc actgtcgcca ccacccgccc
    23341 cgaaacgatg ctcggcgatt ccgccgtagc tgtgcatccg aacgatgaac gctacaaaga
    23401 cataattgga aaatctgttg ttttgccgat tacggaacgc ctcattccta tcatcgccga
    23461 tgaatattct gatcccgaaa agggcacagg cgcggtgaaa atcaccccgg cgcacgattt
    23521 caatgacttc gaagtaggca agcgccacga tctgccgatg atcaatatct tcgaccgtca
    23581 tgcctgtatt gccccgctgg aaagcatacc gagcgaatat cacggcctcg accgctacga
    23641 tgcgcgtaag aaagtgctaa aagaatttga ggaactggat ctgctggtcg aggtcgaaga
    23701 tatcaaaaac gccgtgccct atggcgaccg ctccggcgtg gtgatcgaac cgtttttaac
    23761 agaacaatgg ttttgcgata cgcccacact ggccaagcct gcggctcaag ccgtacgcga
    23821 tggccgtacg cgttttgtgc cccagcaata cgaaaatatg tatttcgctt ggatggataa
    23881 tcagctcccc tggtgcattt cgcgccagct ctggtggggg caccaaatcc ccgcatggta
    23941 cgacgaagac ggcaacgtct atgtcgccga aactgaagaa gaggcacaag cacaggcagg
    24001 cacaggcgtt aagctcaccc gcgacgaaga cgtgctcgat acatggtttt cttccgcact
    24061 gtggccgttt tccacccttg gctggcccga tgacggcgaa gaggcaaaac gcatgctgaa
    24121 aaaatattat cccggtgatg ttcttgtcac agggtttgac atcatcacct tctgggtttc
    24181 ccgcatgatg atgatgagcc tgcatttcat gggcgaggtg ccatttaagg atgtgtacgt
    24241 acatgcgctg gtccgcgatg ctaagggcca gaaaatgtcc aaatccaagg gcaatgtcat
    24301 ggacccgctc gaactcagcg agaaatacgg cacagatgcc ctgcgcttta cgcttgctgc
    24361 catggccgca caagggcgcg acattcgcct ggccgatgaa cgcctgcaag gctaccgcaa
    24421 tttcgccacc aaactctgga acgcttcacg ctactgcgag atgaatggct gcaaggccat
    24481 cgcaggcttc gacccggcca aggtcgagta cacgccgaat caatggatta tcacggccct
    24541 gacagaagcc aaagttgaaa ttgaaaacgc cttagacgca tatcgcttta atgaagccgc
    24601 caatgcgatc tatcaattca cctggggcac attttgcgac tggtatctag aattcaccaa
    24661 acctgtattg caaagtggag gagacgctgc tgaggaagta aaacaaacca ccggttgggt
    24721 gctcgaacaa atcctgattc tgctcaatcc tttcatgccc ttcatcaccg aggaattaca
    24781 tgaagcgttg accggcagag atcagcagat catcgctatg aattggcccg aatatgatga
    24841 gagactaggt tctccagctg cgcgtgagaa aatggattgg ttgatccgca tgatctccga
    24901 aatccgctct gtacgcgctg acatgaacgt gcctgcaggt gcaaaaattc agcttttggt
    24961 caaagatgcc tgtgcggata caaaagccgg gctggaaatc ttcggagaca tcatgaagca
    25021 aatggcgcgc cttgaaagca ttgaacatgt cgaggatgtt cccaaaggcg caattcaaac
    25081 cctggtgggt gaaaccacgt tgattttgcc aatcgccgat attatcgatc tcgatgccga
    25141 acgtatgcgt ctcaaaaaac gcattgataa actcgaagat gacattaata aaatatctca
    25201 acagcttgat aataaaaaat ttattgagaa cgctccagag gaaatcattg ctgaacaaaa
    25261 agaacttgtc gctgaaaaac aaagcgctcg caaaaaactc accgccgccc tccagcagct
    25321 cgaagcggct taattctcag ttccttgcat gagccaagca ataaaaaacg ccctcaaagc
    25381 ggggcgtttt tgtattccgg cgctgtaaaa agcggtctat ttcttatcat tggccttttc
    25441 agacttctgg tcctctgcca gaccaaaatt cccaaaacgt ttttcaaact tggaaagctg
    25501 gcctttctcg atcactttac ccgttccacc ttgccacgca ggatgtgtca acggatcaac
    25561 gtcgagcttg attacatcac cttttttgcc ataggtgctg cgggttgtgt attcgcttcc
    25621 gtctgtcatc acaactttaa tgtcatggta gtcggggtga atatcggctt tcatatcgta
    25681 tctcctattg atcgaggcga atatttatcg tttatagccg gggaatgcaa gtattttctt
    25741 gaaaaacaaa gggcgcaggc atgaattggt ggcttccgca taaatttgaa accaaaaaaa
    25801 catatctcga agtgagagtg caacttatcc gggccatgcg cgcgcatttc gatgatcagg
    25861 atttttggga agtccaaacc ccaatattgc aaaccatgcc cggcgcggat gttcatattc
    25921 atgcctataa acttgaaaac ggcgcttacc tccacaacag ccccgaattc gcgatgaaaa
    25981 aacttctcgt tgccggcgca ccgaaaattt atcaaatatg tccagttttt cgccgtgaag
    26041 agcgcacaaa tctccataac cccgaattta cgatgctgga atggtacagg gcacaggccg
    26101 attacaccaa catcatgaaa gattgcgaag aattactgcg cagtctggaa atcaaggagt
    26161 ttcattataa cggccaaacc tgcgatcccc acgctccctg gaggcgcata accgtctgcg
    26221 aagctttcaa gcaatatacc gatattgatc ttgcatctca cttcggtaac gtagatactt
    26281 ttcgctccac tgtcgccgaa aaaggcatcc gcaccatcga aaccgacaac tgggacgaca
    26341 tcttccacgc cgtgatggcg gagaaaatcg agccgcatct ggggcagggg gctccgacta
    26401 tcctgtatga ttaccccgca tccatggccg tgctttcacg caagaagccc gaagatccgc
    26461 gcctcgccga acgttttgag ctatatgtct gcggcgttga gctcgccaac gcttttagcg
    26521 aactcaccga tccagccgaa caacgcgccc gcttcatcgc tgatatggcc gaaaaacaac
    26581 gcctctacgg cgaaagctat ccgctcgatg a
//
LOCUS       DAOSWR010000008        26376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1179_length_26376_cov_4.891000, whole genome shotgun sequence.
ACCESSION   DAOSWR010000008 DAOSWR010000000
VERSION     DAOSWR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 26376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26376
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_26376_cov_4.891000"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PK513_01315"
     CDS             complement(<1..854)
                     /locus_tag="PK513_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOO81122.1"
                     /translation="MRRVVITGIGIISCIGNNQDEVLESLKAGKSGIIAAPEYAEMGF
                     RSQVHGKPDIDLAANIDKRLWRFMGDAAGYVHLSMEQAIDDAGLGEDLISNERTGIIV
                     GSGGPSTRAQVLAADVTREKGPKRVGPFAVPKAMCSTTSATAATNFKIKGVNYSISSA
                     CSTSAHCIGNAAEQIQWGKQDIMFAGGGEELDWTLSVLFDAMGAMSSKYNDTPEKAAR
                     AYDVDRDGFVIAGGGGIVVLEELDKFKKGTDQIHFQAREFVRNMNEIAGEKLFSEGVS
                     LGAKLGKL"
     gene            complement(940..1473)
                     /gene="fabA"
                     /locus_tag="PK513_01320"
     CDS             complement(940..1473)
                     /gene="fabA"
                     /locus_tag="PK513_01320"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HOO81123.1"
                     /translation="MTSNLHAGHQQSSYSYEEIIKCGEGELFGPGNAQLPLPPMLMFD
                     RITHVSEDGGEYGKGHIAAEFDIKPDLWFFACHFKGDPVMPGCLGLDALWQLTGFFLG
                     WTGAEGSGRALGIGELKFTDQVLPSTKLVRYIVNFKRVINRKLVLGVADGKLEADGQV
                     IYEAKDMKVGLFDNSKG"
     gene            complement(1576..2550)
                     /locus_tag="PK513_01325"
     CDS             complement(1576..2550)
                     /locus_tag="PK513_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81124.1"
                     /translation="MFALTNKPDMGARLYSGLIRMATDPSRGTDGMKLIQHIAGVLVE
                     TYLVFDEPDKAMEASLQELSGMMGCRPAAGALGQGVLPPANIIDLETEKGRAAARAFF
                     EEWLDCAFEFHKLMLVIVHNILISWEAEGLPRAESLRLLIECAHKAMGFELAAQELCD
                     VVIDRKVASEGWGLGDCIASLSAVAGRRLALSLNTDACMIFRGCNLPDNLDHVVFVMT
                     QEAVRLGVPAGTDWRFGLAANDVPVNAPLDLIFGIEPYCQSFFNAINMNCQYDQSVSC
                     AKAAGRMLAVAAGGEVPEMEPAIAKPLAMAAMTETYKSVCMEYEIVSL"
     gene            complement(2653..3153)
                     /locus_tag="PK513_01330"
     CDS             complement(2653..3153)
                     /locus_tag="PK513_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO81125.1"
                     /translation="MRIPILTLWLLIFSLSVAAQEPSKAFRATDLPLPRFVSLHSGEV
                     YVRSGPGQKYPVQWILKKKGLPLEIILEYDIWRKVKDYDGHEGWVHKSMLSGRRTGFI
                     HNDKMVSIHRKPNNKSKIHVYLEPEVLVEIKQCGEGWCHVDAAGYKGWLPQKFIWGVY
                     EDEEFD"
     gene            3246..4238
                     /gene="dusA"
                     /locus_tag="PK513_01335"
     CDS             3246..4238
                     /gene="dusA"
                     /locus_tag="PK513_01335"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HOO81126.1"
                     /translation="MNDLPEISVAPMMDWTDRHCRYFHRLIAPHVRLYTEMITTGALL
                     HGDRERFLRFDESEHPVALQLGGSEPEDLVRCADIVACSGYDEINLNCGCPSERVQKG
                     AFGACLMKEPDLVSDCVRSMIRAVDIPVTVKCRIGIDEQDDFEFLDRFVCMVADAGCQ
                     TFIIHARKAWLQGLSPKQNREVPSLNYERVAAIKEKNPALRIILNGGITDTGTILAQC
                     ERLNGVMIGREAYSNPYFLAEIEQEIFGNEPLWKRDDIAKKMAHYAQEQANRHGTPVK
                     SITRHILGLYHHQAGAKAWKQALSTLPYEDGAGPEVIEAALKAKNKAVATQRTA"
     gene            4286..5416
                     /locus_tag="PK513_01340"
     CDS             4286..5416
                     /locus_tag="PK513_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81127.1"
                     /translation="MADSGDWQLLDTYETKKFIKEVSDPAFGGLFDGPGYDLWTRDLQ
                     FLDGYGHYLLCNKGTFPYFALHYISNGEDHFYLDGSEHPLELLIRHGCLCLDKDNVMD
                     YIGFHSDVTFYPYRKVKFIIDPSKTPYSGASAMGHHFKTLKHHAKFDLHESAEDACFY
                     VHMPLLYNGETVGGYVQVMKSGQINILEPVKIPLMDGKREHAPLDYDHLHEKDLLAQN
                     LDILMQSEEGKRLWETIKSYNGELKFVSGVGSNGLAIASRSTGYIVAPENIETCSPYQ
                     IITMIGVLREMELMLMGKKRPDPHGELHEVLEQHLIINLEILLEICIIGDELASAGHE
                     DILRKFKESGFGDFYSGYKNEVSGEDLVRIAARIFELKVIEE"
     gene            5413..5709
                     /locus_tag="PK513_01345"
     CDS             5413..5709
                     /locus_tag="PK513_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81128.1"
                     /translation="MSTTLGLASQAASKGASSSFSSGFMSMPPTQFTGHDHGYIRLGH
                     IPHESFGLKRNRYRPIMTFGNPADFVGGPRNAGDSATLQHSQGGGSFATATRQT"
     gene            complement(5706..6062)
                     /locus_tag="PK513_01350"
     CDS             complement(5706..6062)
                     /locus_tag="PK513_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOO81129.1"
                     /translation="MNIKITNAAAAQIKTLMGKAPQDAIGLRLNVKTTGCSGNSYAME
                     YADESSKADDRIESNGATIYISKSYSWMLIGTTIDYIADELGNSRFDFQNPNETGRCG
                     CGESFQVERKEEGKEA"
     gene            complement(6069..6479)
                     /locus_tag="PK513_01355"
     CDS             complement(6069..6479)
                     /locus_tag="PK513_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOO81130.1"
                     /translation="MSQQQKKTEKEPCSHVYDELAEPQEADVPREHPLWPAMLAALRE
                     IYDPEIPVNVYELGLIYAVKIAEKNGGVHDVEVKMTLTSPGCPVAQEMPGWVQGALLP
                     VDGVGEVDVEIIWDPPWTPAMMAETAKMQLNMFM"
     gene            complement(6476..7699)
                     /locus_tag="PK513_01360"
     CDS             complement(6476..7699)
                     /locus_tag="PK513_01360"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOO81131.1"
                     /translation="MNVSRNYRDDFPVLATQMNGKPLAFLDTAASAQKPQSVINAMNK
                     VLETGYSNIHRGLYRISQDLTADFEAVRAKIARFIGAKSEKEIVFTRNATESINLVAQ
                     TWGRTHMKQGDEIILTEMEHHANIVPWQLLQKEIGFTIKVIPVRDDGALDLDAFETLL
                     SEKTRLVSIVQISNAFGIINPINKIITLSKKFNPEIKVLIDGSQGIVHQKIDVKTLGA
                     DFYVFTGHKLYGPTGVGVLYGRYDVLETVPPYQGGGDMIERVSFDGTTFKEPPYRFEA
                     GTPAIVEVIGLGAAIDYVQRVGYEHIIMHEAALRDYGHERLSEIDGLKLYGLNTEKTG
                     IFSFTMDCAHPSDIGMILDQCGVAVRAGHHCCMPLMQRFGLDATVRASLGLYSNRQDI
                     DQLLEGLYKVKELFV"
     gene            complement(7696..8652)
                     /gene="sufD"
                     /locus_tag="PK513_01365"
     CDS             complement(7696..8652)
                     /gene="sufD"
                     /locus_tag="PK513_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOO81132.1"
                     /translation="MNFLELKNLPTPKDETWKYTNLGRTLPANLVLADKPQNIVIHKN
                     RGQICEQPEDILFTAFENQHTQPRLKIVLEDGAQATMIERHNGEGAYWKNMVTEIELG
                     QGAILHHIRIQNDAPKAVNTNMVSLKAARDAVYNGFALNSGAKLSRHEIHAIIEGENA
                     EVSFNGVNLLNGEQHGDTTILIEHQAPNGRSNQFYRSLLDDKAHGVFQGKVHVHQIAQ
                     KTDGYQLSNALLLSPEAEMDTKPELEIYADDVKCSHGATTGQLDEEPLFYLRQRGLNE
                     AQGRFLIIQAFVDEVVDKIVDKKIRSEILEVTQTWLHTALKI"
     gene            complement(8933..9694)
                     /gene="sufC"
                     /locus_tag="PK513_01370"
     CDS             complement(8933..9694)
                     /gene="sufC"
                     /locus_tag="PK513_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOO81133.1"
                     /translation="MLHIKDLHASIETDDGTVDILKGISLDVQAGQVHAIMGPNGSGK
                     STLSYVLAGREDYDVTGGTVELGGTDLLDLKPEERAAAGLFLAFQYPVEIPGVNMSSF
                     LKTAINSIRKQRGEDELDALAMLKRIKSTCARLGISDDMMKRAVNVGFSGGEKKRNEV
                     LQMAMLEPKFCVLDETDSGLDIDALKVVADGVNAMRSPDRGFLVITHYQRLLDYIQPD
                     VVHVLAKGKIVKTGGPELAKELEDKGYAEFLDEAA"
     gene            complement(9710..10099)
                     /locus_tag="PK513_01375"
     CDS             complement(9710..10099)
                     /locus_tag="PK513_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81134.1"
                     /translation="MMIADYINTGDPFLLFFGGFYLVLGFSCFFARKPWEEFTALFID
                     NDALSLVAGILILPISLSIIAFYNNWETLASTILMVIGYLAFLKSLVLLMRPSLIQAF
                     VQKEFVRKWIWLDGISGVVLGLALLLL"
     gene            complement(10143..11618)
                     /gene="sufB"
                     /locus_tag="PK513_01380"
     CDS             complement(10143..11618)
                     /gene="sufB"
                     /locus_tag="PK513_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOO81135.1"
                     /translation="MSISEKTIATVADMAGRYEAGFVTDIETDKAPKGLSEDIVRFIS
                     TKKGEPEWMLEWRLKAYCRWLEMPEPEWAKVEFSPIDYQDAYYYAAPKSTGDGPKSLD
                     DVDPKLLETYEKLGIPLREQEILAGVQDRTPVAVDVVFDSVSVATTFKEKLKESGVIF
                     CSISEAIKEHPELVKKYIGSVVPPGDNKHACLNSAVFTDGSFVYIPPGVRCPMELSTY
                     FRINELNTGQFERTLIIADKGSYVSYLEGCTAPMRDENQLHAAVVELVAHDDAEIKYS
                     TVQNWYPGDFKTGQGGVYNFVTKRALCEGRNSKVSWTQVETGSAITWKYPSCILKGDN
                     SQGEFYSVAITNGRQQADTGTKMIHIGKNTKSRIVSKGISAGHSNSTYRGLVKILSKA
                     EGARNFTQCDSLLIGDQCGAHTVPYIESRNKSANLEHEATTSKISEDQLFYCMQRGIS
                     EEEALALIVNGFAREVMQHLPMEFAVEAQKLVGISLEGSVG"
     gene            complement(11615..12058)
                     /locus_tag="PK513_01385"
     CDS             complement(11615..12058)
                     /locus_tag="PK513_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HOO81136.1"
                     /translation="MIKISRLADYAVVTLAELAQQKTPVSAVVVAGQTRLPEPTVAKV
                     LKLLAKAGLVQSIRGVNGGYQLKKPPAQIDVAQIVAAIEGPVSLTACVEGSTDICNYQ
                     KHCSVKGRWDDVHSTLRKALESVTLADMMARPKGCSQMEKRYKCP"
     gene            12219..12479
                     /locus_tag="PK513_01390"
     CDS             12219..12479
                     /locus_tag="PK513_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81137.1"
                     /translation="MSGLVGPEGTDIHYHVAHGIVLTYNKKLLREFEKNNWGPGISRK
                     GVKTWTDDYSNIVSVIGNKTVAIRLKLSNPELYEDKAAISSP"
     gene            complement(12463..14034)
                     /locus_tag="PK513_01395"
     CDS             complement(12463..14034)
                     /locus_tag="PK513_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOO81138.1"
                     /translation="MLENIIRNRRTFAIISHPDAGKTTLTEKLLLFGGAIELAGAVKA
                     KGDRRRARSDWMKVEQERGISVASSVMTFVNNDITYNLLDTPGHEDFSEDTYRVLTAV
                     DSAIMVLDCAKGIETQTRKLFEVCRLRDMPIITFINKMDRDGQDPFDLLDEIEQSLAL
                     DVTPASWPIGMGRDFKGCYDLLNDQLILFDRSKGESLKEGIKCSGLDDPKLDELLTQN
                     EVAKLREDIEMVRGLCPEFDKQAYLDGTMTPVFFGSAVNNFGVRELLNGIGVFAPCPH
                     EQKTRDRIVFPHEKKVSAFVFKIQANMDPKHRDRMAFARICSGKFKRGMKLKHVRSGK
                     TQVIHSPQLFFAQDREMAEEAFPGDIIGLPNYGGLRIGDALTEGEDLVFTDIPSFAPE
                     LMQKARPEDPLKAKHLGKALEQLAEEGAARVFKPAIGSDWVVGVVGALQFEVLRDRIR
                     TEYEIPVKFEQTELYTARWLSSADPQKLKKFVDSNQSAIANDHDGDPVFLARNTWHLG
                     DAQEKNPDIEFKATK"
     gene            complement(14043..15047)
                     /locus_tag="PK513_01400"
     CDS             complement(14043..15047)
                     /locus_tag="PK513_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81139.1"
                     /translation="MKSIKLLIIIVVIVGVAGVLGLFGAKQALEKQASEKVAAIIEEN
                     AQQEDLQTKLMNTARFAYEQYKTAEKKDSKSPVALMKQVLNWKELPASLQLSEEMKDV
                     FGLQAGSEESAAHFLVYLLKQQGLESGIWTIISPKATKLAVGVKVDAGGYKYLDPASG
                     VIAMYQDKIMVGPYAARYLMVDGADYRQVFVKLDEDSDTSFYKDFAHTIMAAPGMPLH
                     VNLAAPVFDEPLVLGAVDGESQDVAEASEEVQLTAYIDYIGQKHGENVHRSFYFTDAT
                     KMTMILTKNYNPGILNANIKPQVEGNKLVFSIPEGEKLTLEDLPGAYIPVDQIIIERL
                     "
     gene            complement(15044..15391)
                     /locus_tag="PK513_01405"
     CDS             complement(15044..15391)
                     /locus_tag="PK513_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HOO81140.1"
                     /translation="MSAQIPTALFVEGHLANLTAKAVSYYFIQKGNAASGLILVKITN
                     INGRARLLVQQRDFTHDCLVWRAALEEETVEERDADAYIKRAISRDPDLWVLEIEDET
                     LANPFEEASRIES"
     gene            complement(15391..15540)
                     /locus_tag="PK513_01410"
     CDS             complement(15391..15540)
                     /locus_tag="PK513_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81141.1"
                     /translation="MRTYKAMQSEPPHVAREAALRVYCYHHPEDAKSDAYLTVERWII
                     AEHIH"
     gene            complement(15614..16024)
                     /locus_tag="PK513_01415"
     CDS             complement(15614..16024)
                     /locus_tag="PK513_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOO81142.1"
                     /translation="MPDLEERCASAGLKMTGQRRVILKVLGEADDHPSVEDVYDRAKG
                     LDSSISIATVYRTLSLLDELDLVVRHEFQEGYSRYEVNADHHHHLVDLETGKVIEFQN
                     EELEKLKIKIAKDLGYDLVDHRLELYGKKTKKKK"
     gene            complement(16118..17623)
                     /locus_tag="PK513_01420"
     CDS             complement(16118..17623)
                     /locus_tag="PK513_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO81143.1"
                     /translation="MSKEYLKFFNEMRIPRFIVKQASDGAYCVEATNQLGLDYFGSDK
                     KQVVGQNIKKFMNTENAIHFEQSFEVAISKKRSVTIQSLPSVPGTVRVHGFYISPILD
                     EEGNVEYLDVIGQFDVADQSIVQRERDDAISLLTSIFEVSEVGIVVTDVNARVVRVND
                     SFIRAFGWSRDELVNQEVISIITPDERELARRNHEEYIRSGLRSSGEMKIIRKDGSIA
                     NALYTTATLELSQKRRFQVTTVMDITLRKQMEQSLRMAKDQADAANRAKSVFLANMSH
                     ELRTPMNAILGFSEMMIKETFGPLGHEKYKEYIGDVHSSARHLLSIINEVLDMSKIEA
                     GRIEVDEGEMDVGGLIESVIRMMDSRAFGSNIRLVIKVDKDLPNIKADQRLIRQVLIN
                     LVTNAMKFSEAGKTVTVSAYMLDDKGIQIKVSDQGKGIPDGKIEQAMEPFGQVTSGEE
                     NSTIEGTGLGLPLAKAMVDLHDGTLHIESEVGKGTDVFVTFPAYRTLECKQ"
     gene            complement(17647..18549)
                     /locus_tag="PK513_01425"
     CDS             complement(17647..18549)
                     /locus_tag="PK513_01425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO81144.1"
                     /translation="MAIQKTLNNQLMIIQEFTDEYQFWCQNVLAKSFFLKEQAESTAS
                     PRKFVEWLQDLSLKAIDIDERYDTRRRQLLEAHHGLLTLSSTCEKDITKENFSRFYNN
                     FRAYIIELQNFTQTIVLEEWGFDVFSGLKNNVVMKSGLQIELDRLSREGYPFCVGLAR
                     IDNFAKFEKTAGQEKADELVKTVAELIQKTLRTYDEAYRVSRNHFIMCIKQSDISKGQ
                     RALERLRELLEKSEALAALNDRNNISMSCCVASPIPADKIEDLIDNLYVDLDTQIKEQ
                     GTVLTYQELSPLQRYILSDSEKNA"
     gene            complement(18659..19849)
                     /gene="tgt"
                     /locus_tag="PK513_01430"
     CDS             complement(18659..19849)
                     /gene="tgt"
                     /locus_tag="PK513_01430"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOO81145.1"
                     /translation="MTIAYPNFAYDITHRGAKSGARLGVLKTPHGVIETPNYIFCGTK
                     AAIKALSPAQMIEAKTDIILANTYHLMLQPGADLIEKIGGLHEFMNWNGPLLTDSGGF
                     QVFSLGEGTMANEIKGKNQDKGHENKNLISITEEGCVFRSYVDGRKIKLTPESAMEIQ
                     RKLGADLLMQFDECTPYHVDKDYTARSMEMSMRWGDRCLAEFERHDNGAQGLYGIVQG
                     GVHSDLRRISAEYTKDRPFFGTAVGGCLGGSDEEMETVVSASLPYAHPDRPVHFLGIG
                     QIKDVFRFVRMGVDTFDCVIPTRLARHGSALMKGHPGETINLKNACYREDKTPLDEKN
                     GVSASCDYSKAYIHHLLKSGEILGLQILAQHNVATINRLMREIRAAIRSGTLDKLEKE
                     WRTH"
     gene            complement(19914..20207)
                     /gene="rpmB"
                     /locus_tag="PK513_01435"
     CDS             complement(19914..20207)
                     /gene="rpmB"
                     /locus_tag="PK513_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOO81146.1"
                     /translation="MSRKCMITGKSVMSGNNVSHALNRTRRKFLPNVQDTSLYSEALN
                     KWVKVKASSAGLRTVEHKGGFDAYLTVTAKTKLDPALRPVKAAIEKAKGAKAA"
     gene            complement(20276..21301)
                     /locus_tag="PK513_01440"
     CDS             complement(20276..21301)
                     /locus_tag="PK513_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOO81147.1"
                     /translation="MTNFITQWHVQIMNVDRIPVIVLALLLCAVVGMITGPLAGNAHP
                     FFWRVIDLFFGKLGDRMDRAHRPQADLMFRGLFLSVLVIILATFIGQSFETFAAQTPA
                     YGITRIILLSSLFAAGSIWFVLLKLYFTMENNTVGQGAFYAIARTTRINLAAGDDYGV
                     TRAAVSLSARSFDKGLVAPALWYLIGGFPAACTYVALTALSWRFGKNGLGSGFAAVPL
                     VLERLMGMIPSFFAALLITLAAGLTPTARLHKGIAAWFGHKNRAPYAQGGAPVSALSW
                     ALNVSVGGAVQDLSGTALKGEWVGPEGATAKLDHKHLRRAIYINVMAHILFVAVLLAT
                     YMWGGIL"
     gene            21414..22796
                     /gene="phaZ"
                     /locus_tag="PK513_01445"
     CDS             21414..22796
                     /gene="phaZ"
                     /locus_tag="PK513_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOO81148.1"
                     /translation="MLYQLYEFQHAMMTPLRFQAELMRSAFQNPWNPLSYTQAGRMVA
                     ASAEMIERSTRRFGRPDFGLHETQIDGRKVEVVEKNIVEKPFCRLLNFQRKIKRHDPT
                     VLLVAPMSGHYATLLRGTVAALLPHHNVYITDWEDARQVPLSDGKFSLDTYITYLREF
                     MSLLGPQTHIIGVCQPVVPVLAAVSLMASDDDPNQPLSMTLMGGPIDTRVSKTEVTEL
                     AETRPLSWFEQSVVHDVPFYYPGAHRRVYPGFLQLGGFMSMNLDRHVGSFMQFYQHLV
                     QGDGESAVAHRKFYDEYLSVMDIPAEFYLQTVEIVFQRHLLPRGAMKWRDPLSDELID
                     VRPQDIRKTALLTIEGELDDISARGQTTAAHEICYSIPQRMQYHRFQPECGHYGIFNG
                     RRWRNEIMPRVRHFIREFDPSADPVPKKDLEKVADLSASRYNRDRHGIVAVRRWIKEH
                     RAENYKETID"
     gene            23019..23717
                     /locus_tag="PK513_01450"
     CDS             23019..23717
                     /locus_tag="PK513_01450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOO81149.1"
                     /translation="MLLRQETAFPPYVTLKRSKRARRLALRLDPKDRTFHLVVPAGMS
                     VKKAQRFAEEHDRWMQDKLLDLPEPIPFEDGSILPILGQNRRVHVFYDSTLRTTSIHL
                     KYNELIVTTNKEDPTARIERSLKKRAREELLALSQQKSEAIGKRVKGVGARDTKSRWG
                     SCSSDGNLSYSWRLIFAPYEALDYVVAHEVAHLKYMDHSRAFWNLCRELSDNFIEGQY
                     WMQENGHELMRYGG"
     gene            complement(23772..24083)
                     /locus_tag="PK513_01455"
     CDS             complement(23772..24083)
                     /locus_tag="PK513_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81150.1"
                     /translation="MSNLRQDISNLDIYDIELIFTACSSNNVREGISTVSFPALEEMR
                     AKEGVWLSEARPRSLRARLFDLASRGLLEVVGSDSEEKQLQYQGTALGEKLLLFTDPC
                     L"
     gene            complement(24145..24630)
                     /gene="ruvX"
                     /locus_tag="PK513_01460"
     CDS             complement(24145..24630)
                     /gene="ruvX"
                     /locus_tag="PK513_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO81151.1"
                     /translation="MSVGLLKDMVERRPLNVAMLGLDVGKKTIGLALSDPGQTLATPL
                     QTIRRTRFAKDMEFLNVVITEYDVDGFVLGYPVNMDGSEGPCAQSIRDFAIEMERTLG
                     NDPWIALWDERLSTVSADNIVDKMVQKRKTKVNAKASGLIDKLAAQVILQGALDFMKK
                     Y"
     gene            24720..25016
                     /gene="gatC"
                     /locus_tag="PK513_01465"
     CDS             24720..25016
                     /gene="gatC"
                     /locus_tag="PK513_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOO81152.1"
                     /translation="MSMDEKTVRKVARLANIEIADHDVERIGHELSGILDWVEQLGEV
                     DTENVEPLANVVNIELPLRKDEVTDGNCSEKVLANAPEETQGFFVVPKVVEQEG"
     gene            25018..>26376
                     /gene="gatA"
                     /locus_tag="PK513_01470"
     CDS             25018..>26376
                     /gene="gatA"
                     /locus_tag="PK513_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOO81153.1"
                     /translation="MSKLTYLTIEEALKGLNNKDFTAVELAQAHIDAMEANCDLNAFI
                     METPEIALAQAAESDKRYAASEAKPLDGIPFANKDLFCTEGVQTTAASKILEGFIPQY
                     ESTVSSKLFADGAVMLGKANLDQFAMGSSNTTSAFGNVISPWKATDGKDLVPGGSSGG
                     SAAAVAARMAMGATATDTGGSIRQPASFCGIVGIKPTYGRCSRWGVVAFASSLDTPGP
                     LARTVKDNALLLASMAGHDAKDSTSANRDVPDFASAITGDIRGLKIGIPKEFKLDGMP
                     PEIEKLWEQGMAWLKEAGAELVEISLPHTKYALPTYYIIAPAEASSNLARYDGVRYGH
                     RSTESKTLDDMYKKTRAEGFGDEVKRRIMIGAYVLSAGYYDAYYIKAQKVRRLILQDF
                     EKAYETVDAILTPTAPSGAFAIGENEDDPIKMYLNDVFTVTANLAGIPAMSVPAGLDA
                     NGL"
BASE COUNT         6594 a         6332 c         6436 g         7014 t
ORIGIN      
        1 aaaagttttc cgagcttggc tcccagcgaa acaccttccg aaaagagctt ttccccggca
       61 atttcgttca tattgcgcac aaactcccgc gcctggaaat gtatttgatc agttcccttt
      121 ttaaatttat cgagttcttc gagcaccaca atcccgcccc cgccggcgat gacaaagccg
      181 tcacggtcaa cgtcataggc acgggcggcc ttttccggtg tgtcgttata tttggaactc
      241 atcgcgccca ttgcgtcgaa caaaacagaa agtgtccaat caagctcttc gccaccaccg
      301 gcgaacatga tgtcctgctt gccccactgg atttgttcgg cggcattgcc gatgcaatgt
      361 gcggaggtac tacaggccga ggaaatggag taattcacac ccttgatctt aaaatttgtg
      421 gccgcagtgg cagacgtcgt cgagcacatg gctttaggca cggcgaacgg accgacgcgc
      481 ttagggcctt tttcgcgcgt tacatcggca gcgagcacct gagcgcgggt tgacgggccg
      541 ccagagccga caataatgcc agtgcgttcg tttgagatga ggtcttcgcc aagccctgca
      601 tcgtcgatgg cttgttccat tgagagatgt acatagccag ccgcatcgcc cataaagcgc
      661 caaaggcgtt tatcgatatt tgccgccaga tcaatgtccg gtttgccgtg aacctgtgaa
      721 cggaagccca tttcagcgta ttccggcgcg gcgatgatac ccgatttacc cgcttttaag
      781 ctctcaagca cttcgtcctg attattgccg atgcaggaga taatccctat gcctgtaata
      841 acaacgcgtc tcatggtttt tagctcttag tttgtagttt atagcttctc atgtttagtt
      901 cgtcaaaatt ctaaaaacta caaactaaac actaaaaatt taccctttgg aattatcgaa
      961 caggccgact ttcatgtctt tggcctcgta aatgacttgt ccgtcggctt cgagtttgcc
     1021 atcagcaacg cccagaacca gcttacggtt tatgacgcgt ttgaaattga cgatatagcg
     1081 cacgagtttt gtgctcggca aaacctgatc ggtgaatttc aactccccta ttcccagcgc
     1141 gcggcctgac ccttctgcgc cggtccagcc gaggaaaaag ccagtgagtt gccagagcgc
     1201 gtcaagaccc agacagcctg gcataacagg atcgcctttg aagtggcatg caaagaacca
     1261 caggtcgggc ttaatatcaa attccgcagc gatatggcct ttcccatatt caccaccatc
     1321 ttcagagacg tgcgtgatgc ggtcaaacat caacatgggc ggcagcggca gttgcgcatt
     1381 accgggacca aataattcac cctcaccgca tttgatgatt tcttcgtagc tgtagctgga
     1441 ttgctggtgc cctgcgtgga gattggaagt catgaatcac ccatctggtt tgtgaaaact
     1501 gcacttgatt tatagggaga ttcagggcgc tacgcaagcc ctgtcagaca tctatcggtg
     1561 gatagaatac ggcagttaaa gagaaacgat ttcgtattcc atgcaaaccg atttataagt
     1621 ctcggtcatc gccgccatag ccagaggttt ggcgattgcc ggttccattt caggcacttc
     1681 gccgcctgcg gccacggcaa gcatacgccc ggcagctttg gcgcaagata ccgactggtc
     1741 gtattgacag ttcatgttga tggcattgaa gaagctctgg caatatggct cgatgccgaa
     1801 gatcagatca aggggcgcat tgaccggcac gtcatttgcc gcaaggccga accgccaatc
     1861 tgtacccgca ggtacgccga gacgtacagc ttcttgtgtc atgacaaaaa cgacatgatc
     1921 gagattatcg ggcaagttac agccccggaa gatcatgcag gcatcggtgt tgagggagag
     1981 tgcgaggcgg cgcccggcaa cagcgctcaa cgatgcgatg cagtcaccca gcccccagcc
     2041 ctcggaggcg actttgcggt caatgacgac atcacataat tcctgagcgg caagctcaaa
     2101 acccatagct ttatgagcgc attcgattaa cagacgcagg ctttcagccc gcggcaagcc
     2161 ttcggcttcc cagcttatga gaatattgtg aacgatcacc agcatcagct tgtgaaattc
     2221 aaaagcacaa tccagccatt cttcaaaaaa tgcgcgtgcg gcggcgcggc ctttttccgt
     2281 ttccaaatcg attatatttg caggcggcag gacgccctgc cccagagctc cggctgcggg
     2341 gcgacagccc atcatgccgg aaagctcctg taggctggct tccattgcct tatcaggctc
     2401 gtcaaatacc aaataggttt ccacaagtac gccggcgata tgctggataa gtttcatacc
     2461 gtcagtaccg cgcgatggat ctgtggccat gcggatcaaa ccggaatata aacgcgcgcc
     2521 catgtcgggc ttattggtta atgcaaacat acaaaaaacg ggccttgggg ttaaggctca
     2581 ctcgaactgg gtttgtgaat aaaactgtgg atagatactg tggagggcgc agaatcaact
     2641 gcatgtaaaa gactaatcga attcttcgtc ttcgtaaaca ccccaaatga atttttgtgg
     2701 cagccagccc ttgtaacctg cagcatcaac gtggcaccag ccttcgccgc attgcttaat
     2761 ttcaactaaa acctctggct caagatatac atgtatctta cttttattat taggttttct
     2821 atggatagaa accattttat cattgtggat aaaacctgtg cgccgtccac taagcatgga
     2881 tttatgaacc cagccttcat ggccgtcata atctttcact ttacgccaaa tgtcgtattc
     2941 aaggatgatt tcaagcggga gacctttctt tttcaatatc cattgcaccg ggtatttctg
     3001 ccctggaccg gagcgaacat atacttcgcc tgagtgcaag gatacaaaac gtggcaacgg
     3061 caaatccgtc gcacggaaag ctttggaggg ctcttgtgct gctacagaca gtgaaaagat
     3121 caaaagccag agtgtgagaa ttggaatacg catatgtatt tttagcgcgg cgcacattaa
     3181 aaacgcaatg gtctattgat gaaaattatt gcgaataagg caccccccgc gctaaaactc
     3241 ctgccatgaa cgatttacct gaaatttctg tagcgccgat gatggattgg acggaccggc
     3301 attgtcggta ttttcatcgg ctgattgctc cgcatgttcg actttacact gagatgatca
     3361 caacgggcgc gcttttgcat ggtgatcgcg agcgtttctt gcgttttgat gagagcgaac
     3421 atccggtagc acttcagctt ggcggatctg agcctgagga tctggtacgc tgcgcggata
     3481 tagttgcttg ctcaggttac gatgagatca acctgaattg cggatgtcct tctgaacgcg
     3541 tacaaaaggg tgctttcgga gcttgtttga tgaaggagcc ggatctggtt tctgattgcg
     3601 tcaggtcgat gatccgagcc gtggatattc ccgttaccgt taaatgccgc attggcattg
     3661 acgagcagga tgacttcgag tttctcgatc ggtttgtctg catggtggcc gatgcaggct
     3721 gtcagacctt tatcattcat gcgcgcaaag cctggctgca agggctttca ccaaagcaaa
     3781 accgtgaggt tccgtcactg aattatgaac gggtggcagc gattaaagag aaaaaccccg
     3841 cactgcgcat cattttaaac ggcggaatta ccgatacggg aacaatattg gcgcagtgtg
     3901 aaaggcttaa cggcgtaatg attggccgcg aagcttattc aaacccttat tttctagccg
     3961 agattgaaca ggaaatcttt ggtaatgagc ccctttggaa gcgagacgac attgcgaaaa
     4021 agatggcgca ttatgcgcaa gaacaggcta atcgccatgg cactcccgtt aaatcaatca
     4081 cgcgtcatat tctaggatta taccatcatc aggccggggc aaaagcctgg aaacaggctt
     4141 taagcacgct tccttatgaa gatggtgcag gcccagaggt tatagaagct gcccttaaag
     4201 cgaaaaataa agccgtagcg acacaaagaa ctgcataagt cataaaaatt ggtgtacttt
     4261 aaagacttaa gtatagggat ttattatggc cgatagcggt gattggcagc ttttagacac
     4321 atacgaaacc aagaaattta tcaaagaagt ttctgacccg gcgtttggcg gtctgtttga
     4381 tggcccgggt tatgatctct ggacccgcga tttgcaattt ttagacgggt acggtcatta
     4441 tttgctgtgc aacaaaggca cgtttcctta ttttgccctg cactatatca gcaatggcga
     4501 ggatcatttc tatctggacg gatcggaaca ccctttggag ttgctgattc ggcatggctg
     4561 tttatgttta gataaagata acgtcatgga ttacataggg tttcatagcg atgtaacgtt
     4621 ttacccttat cgcaaagtaa agttcatcat agatccatcg aaaacgccct attccggggc
     4681 cagtgcgatg ggacatcatt tcaagacctt gaaacatcat gcaaaatttg accttcatga
     4741 aagtgcagag gatgcctgtt tttacgtgca tatgccgctg ttatataacg gagagaccgt
     4801 tggcgggtac gtacaggtga tgaaaagcgg tcaaatcaat atcctggagc cagttaaaat
     4861 cccgcttatg gatggcaagc gtgagcatgc accgctggat tacgatcatt tgcatgaaaa
     4921 agacctttta gcacaaaatc tggatatttt aatgcaatct gaggaaggaa aacgtctttg
     4981 ggaaacgatc aagagctata atggtgagct taaatttgtt tccggtgtag gaagtaatgg
     5041 gctggccatt gcctcacgca gcacgggtta catcgttgcg ccggaaaata tcgagacgtg
     5101 cagtccttac caaatcatta caatgattgg tgttttacgg gaaatggaac tgatgcttat
     5161 ggggaagaag cggccagatc cccatggcga gctgcatgag gttcttgagc agcatttgat
     5221 cataaatttg gagattttgt tagaaatatg tataattgga gatgaattgg cctccgccgg
     5281 acacgaagat attttgcgta aatttaaaga gtctggattt ggcgattttt acagcggcta
     5341 taagaatgaa gtctccggtg aagaccttgt ccggattgcc gcgagaattt ttgaactgaa
     5401 agtgatagaa gaatgagcac aacattaggt ttagccagcc aagccgcctc aaaaggcgcg
     5461 tcgagttcgt ttagctcggg ctttatgagc atgccgccca cgcagtttac aggacatgat
     5521 catggatata ttcgactggg gcatattcca catgaatctt ttggccttaa acgcaaccgc
     5581 taccgtccga ttatgacttt tggcaacccg gcggattttg tcggcgggcc gcgcaatgcc
     5641 ggggatagtg caacgctgca acattctcaa ggcggcggat cgtttgcgac tgcaacacgg
     5701 caaacctaag cctctttgcc ctcttctttc cgttcaacct gaaaactttc gccacaaccg
     5761 cagcggccgg tttcgttagg attttgaaaa tcgaaacgac tattgcccag ttcgtcggca
     5821 atgtagtcta tcgttgtgcc gatcagcatc catgaatagc ttttggaaat atagatggtg
     5881 gcaccattgc tttcgatgcg gtcatcggct ttggaacttt cgtctgcata ttccatggca
     5941 tacgagtttc ccgaacagcc ggtggtcttg acgtttaggc gcaagccgat tgcatcttgc
     6001 ggcgctttgc ccatcagcgt tttgatttga gcggcagcgg cgtttgtgat tttaatgttc
     6061 attctgccct acatgaacat attgagttgc atcttggcag tttcagccat cattgcaggc
     6121 gtccatggcg ggtcccatat tatttcgaca tcgacttcac cgacgccgtc aacgggcaaa
     6181 agcgcacctt gcacccagcc gggcatttcc tgagctactg gacagcccgg tgaggtcagc
     6241 gtcattttaa cctcgacatc gtgaacgccg ccattttttt cagcaatttt aacggcgtaa
     6301 atcaggccaa gttcatagac attgactgga atttccgggt cgtagatttc cctaagcgct
     6361 gccagcatag cgggccatag cggatgctca cgtgggacat ccgcttcttg cggctctgcc
     6421 agttcgtcat aaacgtggga gcacggctct ttttctgtct ttttttgctg ctggctcata
     6481 caaataattc ctttaccttg tacagacctt ctagcagttg atctatatcc tgtctattag
     6541 aatacagacc caaagaggcg cgcactgtgg catccagccc aaagcgctgc atcagcggca
     6601 tacaacaatg gtgcccggca cgcacagcga cgccgcattg atcaaggatc atgccgatgt
     6661 cgctggggtg agcgcagtcc atcgtgaagc tgaagatgcc ggttttttcg gtgttaagac
     6721 catagagctt gaggccgtct atttcactga gccgttcatg gccgtaatcg cgcagcgccg
     6781 cttcatgcat gataatatgt tcgtacccta ctctttgcac gtaatcgatg gccgcgccaa
     6841 gaccgatgac ctcgacaatg gcgggtgtac cggcttcaaa gcgatatggc ggttccttga
     6901 aggttgtgcc gtcaaagctg acacgttcga tcatatcacc gccgccctga tatggcggca
     6961 cagtttcgag cacatcatag cgcccgtaca ggacgccaac gccggtaggg ccgtagagct
     7021 tatgcccggt gaagacatag aagtccgcac cgagagtttt gacatcaatt ttctggtgga
     7081 cgataccttg ggagccatca atcaagactt taatttctgg attaaacttc ttcgataacg
     7141 ttatgatttt atttatagga tttataatac caaaagcgtt ggaaatctga acgatggaga
     7201 cgagccgcgt cttttcgctc aagagcgttt caaatgcatc aaggtcaagc gcgccatcgt
     7261 cacgaacggg aataacttta atcgtgaagc caatttcttt ttgcaggagt tgccagggca
     7321 caatattggc gtgatgctcc atctcggtga gaataatctc atcaccctgt ttcatgtgcg
     7381 tacgccccca tgtttgagca acaagattga tgctttcggt cgcattgcgg gtaaaaacaa
     7441 tctctttttc ggatttagcg ccaataaagc gggcgatttt tgcgcgtacg gcctcaaaat
     7501 cggcagttaa atcctgagaa atgcgataaa ggccgcgatg aatgtttgag tagccggttt
     7561 caagcacctt attcatcgca ttgataacgc tttgcggttt ttgagcgctg gccgcggtat
     7621 cgaggaacgc caagggcttg ccgttcattt gcgtggccaa aacaggaaag tcatcgcggt
     7681 aattacgaga cacgttcata tttttagcgc tgtgtgcagc catgtttgcg ttacttccaa
     7741 aatttcgctg cggattttct tatccacaat tttatccaca acctcatcca caaaggcttg
     7801 aatgattaaa aaccgcccct gagcttcgtt taagccccgt tgacgcagat aaaagagcgg
     7861 ttcttcatcg agttgtcctg tggtggcacc atgtgaacat tttacatcgt cggcataaat
     7921 ttcaagctcc ggtttggtat ccatttcagc ctcgggtgag agcagaagcg cgttggagag
     7981 ctggtaacca tcggttttct gagcgatttg gtgtacgtgt accttgccct gaaacacgcc
     8041 atgggcttta tcatcgagca aagagcggta aaactggttg gagcgtccat tgggggcttg
     8101 atgctcgatc agaatggtgg tgtcgccgtg ctgctcaccg ttcagcaggt ttacgccgtt
     8161 gaaggacact tcggcattct ctccttcaat aatggcgtgg atttcatgac gagagagttt
     8221 ggcaccgcta ttcagggcaa agccgttata gaccgcgtca cgggcggctt tgaggctgac
     8281 catattggta ttgaccgcct tgggagcgtc gttttggatg cgaatgtgat gaagaatagc
     8341 gccctgccct agctcaattt ctgtgaccat gtttttccaa taggcgcctt cgccattgtg
     8401 ccgttcaatc atggtcgcct gcgcaccgtc ttcgagcaca atcttcaagc gtggttgtgt
     8461 gtgctggttt tcgaaagctg tgaacagaat gtcctcaggc tgttcacaga tctgtccacg
     8521 gtttttgtgg ataactatgt tctgcggttt atcggccagc acgagattag ccggaagcgt
     8581 acggcccaga ttggtgtact tccacgtttc gtctttagga gttggcagat tttttaattc
     8641 taaaaaattc atcgtttctt ctattcaatg aaaaatgaca acgtcacgat tatagggaga
     8701 acatttgctg gtactaataa aaagggcaac attttcctgg aggggtcact ccacattttt
     8761 ttcaaacaga ataaatctgc ggctgctaat aataaacata tgagccagat aaagaatacg
     8821 gctcctatat ccacttccac tgtcgccatc aatgctaaaa acattacgat cgcaatgatg
     8881 attacattga gaaaaaccag caggcttacg caaattttaa aagcctttaa catcacgctg
     8941 cctcatcaag gaattcagcg tagcccttgt cttcaagctc tttggccaat tctggtccgc
     9001 cggttttaac gatcttgcct ttggccaaca catgtacgac gtcgggctgg atataatcca
     9061 gcaggcgttg gtagtgggtg ataaccagaa aaccgcggtc tgggctgcgc atggcattca
     9121 caccatcggc gacaaccttg agcgcatcaa tatcgaggcc gctgtcggtt tcatcgagaa
     9181 cgcaaaattt tggctctagc atcgccattt gcaggacttc gttgcgcttt ttctcaccgc
     9241 cggaaaagcc tacattgaca gcgcgtttca tcatatcatc agaaatgccg agcctggcgc
     9301 aggtcgattt gatgcgtttc agcatggcga gtgcgtccaa ttcatcctcg ccgcgttgtt
     9361 tacggatgga attgattgca gttttgagaa aggagctcat atttacgccg ggaatttcaa
     9421 ccggatattg gaaagcaagg aacagaccgg cggcggcgcg ttcttccggc ttcagatcca
     9481 gaaggtccgt tccgcccaac tcgaccgtac cacctgtaac gtcataatcc tcgcgcccgg
     9541 ccagtacgta tgagagcgtg gacttgccag agccgttggg gcccataatc gcgtgaacct
     9601 gcccggcctg gacatcgagc gaaatccctt tcaggatatc gactgtgcca tcgtcggttt
     9661 cgatagatgc atgaagatct ttgatatgca gcattacttt tccttaaggt tataacaata
     9721 gcagcgccag cccgagcacg acgccggaga tgccgtcaag ccatatccat ttgcggacga
     9781 attctttttg cacaaaggcc tgaattaaac tgggacgcat taacaaaaca agagatttga
     9841 ggaacgccag atagccgata accattaata tggttgaagc cagtgtttcc caattattgt
     9901 aaaaggcgat aatcgagagc gaaatcggca agatcaaaat gccagcaaca aggcttaagg
     9961 cgtcattgtc gatgaaaagt gctgtaaatt cctcccaggg cttacgcgca aagaaacatg
    10021 agaaccctaa aaccaggtag aacccaccga aaaacagcag gaacgggtca ccggtgttaa
    10081 tataatctgc aatcatcatt tttatgctcc ttattcaaaa atatgtgcct ttcagtgtac
    10141 acttatccaa cgcttccttc cagagagatg ccaacgagtt tttgcgcctc gacagcgaat
    10201 tccatgggca agtgctgcat aacttcgcgg gcaaagccgt tgacgatgag agccagcgct
    10261 tcttcttcac ttattccgcg ctgcatacaa taaaagagct gatcttcgga gatttttgag
    10321 gttgtggcct catgctctaa attcgcagat ttattgcggc tttcgatata ggggaccgta
    10381 tgcgcgccgc attgatcgcc aatcagaagg ctgtcgcatt gggtaaagtt acgcgcacct
    10441 tcggctttgg acaaaatctt aacaagcccc ctgtacgtgc tgttggaatg gccggcgctg
    10501 atcccttttg agacgatgcg acttttcgtg tttttaccga tatggatcat tttagtgccg
    10561 gtgtcggctt gctggcggcc attggtgatg gccacggaat aaaattcgcc ttgggaatta
    10621 tcgcctttga gaatacagga tggatatttc caggtaatcg ctgatccggt ttcaacctgt
    10681 gtccagctta ccttcgaatt gcgcccttca cataaagcgc gtttggttac gaaattgtag
    10741 acgccaccct gccctgtttt aaaatcaccg ggataccagt tttgtacggt ggagtatttg
    10801 atttcggcat catcgtgagc aaccagctcg accacagcgg cgtggagttg attctcgtca
    10861 cgcatgggcg cggtgcaccc ttccaaatag gatacgtacg atcctttatc cgcgataatc
    10921 agcgtacgtt caaactggcc cgtattgagt tcgttaatgc ggaaataggt ggacagttcc
    10981 atcgggcagc gtacgcccgg tggaatatag acgaaggagc cgtcggtaaa gactgcactg
    11041 ttgaggcagg catgtttatt gtcaccgggc ggaacaaccg agccgatata tttttttacg
    11101 agttcagggt gttccttgat ggcttcagaa atagagcaaa aaatgacccc tgattctttg
    11161 agcttttcct taaaagttgt cgcgacggat acagagtcga acaccacatc aacggcgacg
    11221 ggcgtacgat cctgcactcc ggcaaggatt tcctgctccc gcaggggaat gccaagtttt
    11281 tcgtaagttt caagcagttt gggatcaacg tcatccaggc tttttggccc atcacctgtg
    11341 cttttgggcg cggcgtagta ataagcgtct tgatagtcga tgggtgaaaa ttcaaccttg
    11401 gcccactccg gctcaggcat ttccaaccac cggcaatagg ctttgagacg ccactccaac
    11461 atccattcag gctcgccctt ttttgttgaa ataaaacgta caatgtcttc agacaggcct
    11521 ttgggcgctt tatccgtttc aatatcggta acaaaaccgg cctcgtagcg cccggccata
    11581 tccgcgacgg tggcgattgt tttttcagag atgctcatgg gcacttatac cttttttcca
    11641 tttgagaaca cccttttggc cgggccatca tatcggccaa tgttacagat tcaagtgctt
    11701 tacgcagcgt actatgtaca tcgtcccagc ggcctttgac gctacaatgc ttctgatagt
    11761 tacaaatatc agtgctgccc tcgacgcagg cggtgagtga aacaggccct tcaatggccg
    11821 caacgatttg tgcgacgtca atttgcgcag gcggcttttt gagttgatag ccgccattaa
    11881 caccgcgaat tgattgcacc aatcccgctt tggccagaag tttaagcact tttgcgactg
    11941 taggttcggg caaacgggtt tgccctgcca ccaccacagc gctgactggc gttttttgct
    12001 gtgcgagttc tgcaagagtg actacagcat aatctgcaag acgcgatatt ttaatcatta
    12061 cccttaatcc ggactagaaa tgtacgattt aaatgtcatg gctggctgcg gatttcaagg
    12121 gaaaaactgt gaaaaatttg gataaattta caaaaataac tctttgaatg aacctccacg
    12181 ggccaaggcc cgtggtatca ctctaattca agctgcgctt gtccggactc gttggtccgg
    12241 agggtacaga tattcattat catgtggctc atggcattgt actgacgtac aataaaaaac
    12301 tacttcgtga gtttgagaaa aataactggg ggcctggaat ttccaggaag ggtgtaaaaa
    12361 cctggaccga cgattattca aatattgtca gcgtgatcgg taacaaaact gtagctatac
    12421 gccttaagct cagtaatcct gagctttatg aggacaaagc cgctatttcg tcgccttaaa
    12481 ctcgatgtcg gggtttttct cctgcgcatc gccaaggtgc caggtgttgc gggcgagaaa
    12541 aactggatca ccatcatggt cattggcaat cgcgctttga ttggaatcaa cgaatttttt
    12601 tagtttttgc ggatctgcag atgaaagcca acgggcggtg taaagctctg tttgttcaaa
    12661 ttttacagga atttcatatt ctgtgcggat acggtcgcgc agcacttcaa attgcagggc
    12721 gccgaccaca ccaacgaccc aatctgaccc tattgcaggc ttgaacaccc ttgctgcgcc
    12781 ttcttcggcc agttgctcca aggctttacc aagatgtttg gctttgagcg ggtcttccgg
    12841 gcgggctttt tgcatcagtt caggtgcgaa actcggaatg tctgtaaaaa ccagatcttc
    12901 gccttcggtt agcgcatcac caatgcgcaa gccgccatag ttgggcagac ctataatatc
    12961 gccgggaaag gcttcttcgg ccatttcacg gtcctgagcg aagaaaagct ggggcgaatg
    13021 gatgacttgc gttttgccgg aacgcacatg tttgagcttc ataccacgtt taaacttgcc
    13081 agagcatatg cgagcaaagg ccatgcggtc gcggtgtttg gggtccatat tggcttgaat
    13141 tttaaaaaca aaagcggaga cttttttctc atgcggaaag actatgcgat cgcgggtttt
    13201 ttgctcatgc gggcatggag caaaaacgcc gataccgttc agaagttcgc ggaccccgaa
    13261 gttattgacg gcagagccga agaagacggg cgtcattgtg ccatcgagat aggcttgttt
    13321 atcaaattca ggacacagac cgcgaaccat ttcaatgtct tcgcgcaatt tggcgacttc
    13381 attttgcgtg agcaattcat cgagctttgg atcatcaagg ccagagcatt taatgccttc
    13441 tttcaggctt tcacctttgg agcgatcaaa caaaatgagc tgatcattca gcaaatcata
    13501 gcagcctttg aaatcgcgac ccatgccgat gggccagctg gcgggcgtaa catcgagggc
    13561 aaggctttgt tcgatttcat cgagcaagtc gaacgggtcc tggccatcgc ggtccatctt
    13621 attgatgaag gtgataatcg gcatatcgcg caggcggcag acttcgaaca atttgcgggt
    13681 ctgggtttcg atacccttgg cgcaatcgag taccatgatg gcactgtcca cggcagtaag
    13741 gacgcggtaa gtgtcttcag agaaatcctc gtggccgggc gtatcgagca gattataggt
    13801 aatgtcatta ttcacgaaag tcatgacgga tgaggcgaca gaaattccgc gctcttgctc
    13861 gaccttcatc cagtcggagc gcgcgcggcg ccgatcgccc ttggctttaa ccgctccggc
    13921 caattcaatc gcaccgccaa agagcaacaa cttttcggtt agcgttgtct tcccagcatc
    13981 agggtgagaa ataatggcaa aagtgcgccg gtttctaatg atgttttcga gcataaagag
    14041 gcttataggc gctcaatgat aatctggtca accggaatat aagcgccggg caaatcttcg
    14101 agtgttaatt tttcaccttc agggatgcta aaaactaact tattgccttc gacttgcggc
    14161 ttgatattcg cattcaaaat gccggggtta taattttttg tcaaaatcat cgtcatttta
    14221 gtcgcgtcag taaaatagaa tgagcgatgc acattctcgc catgtttttg gccaatgtaa
    14281 tcaatataag ccgttaattg aacctcttcg gaggcttctg caacgtcttg tgattcacca
    14341 tcgacagcgc ctaaaaccaa tggttcgtca aagacgggag ctgccaaatt tacgtgaagc
    14401 ggcataccag gcgctgccat gatcgtatga gcaaaatctt tgtaaaaaga tgtatcagag
    14461 tcttcatcga gtttaacaaa gacctgccga taatctgcac catcgaccat aaggtagcgt
    14521 gcagcataag ggccaaccat tatcttgtcc tggtacatcg caattacgcc gctggccgga
    14581 tcaagatatt tatatccgcc tgcatccacc tttacgccaa ctgcgagctt cgttgctttg
    14641 ggggaaataa tcgtccaaat tccagattcc agtccttgtt gttttaggag ataaactaga
    14701 aaatgcgcgg cactttcctc cgagccggct tgcaagccaa agacatcttt catctcttca
    14761 gataattgca aagaagcagg cagttctttc caattcaaaa cctgtttcat aagagcaaca
    14821 ggacttttgg aatctttctt ttccgcagtt ttatattgct catatgcaaa gcgcgccgta
    14881 ttcataagtt tggtttgcaa atcttcttgt tgtgcatttt cctcaataat tgcagcgact
    14941 ttttcggaag cctgtttttc cagcgcttgc tttgctccga agaggccaag cacgcccgcg
    15001 acgcctacaa tcacaacaat aattattagc agcttaatac ttttcatgat tcaatcctgc
    15061 tcgcttcttc aaaggggttg gccaatgttt cgtcttctat ttcaagaacc cataagtccg
    15121 ggtcccgtga gattgcccgt ttaatgtacg cgtctgcgtc gcgttcctca actgtttctt
    15181 cctcaagggc ggctctccac accaaacaat catgtgtaaa atcacgctgt tgtaccaaaa
    15241 gtctggcacg gccgtttata ttcgtaattt tgacaagaat caggccagag gcggcgtttc
    15301 ctttttggat gaagtaatag gacacagctt tggctgttaa attggccaga tgcccctcga
    15361 caaaaagtgc tgttggaatt tgggcagaca ttaatgaatg tgctcggcaa taatccaacg
    15421 ctcaaccgtt aagtatgcat ctgattttgc atcttcagga tgatgataac aataaacgcg
    15481 caaggcggct tctcttgcca cgtgcggagg ttcgctttgc atggctttat atgtacgcat
    15541 aaccgcgcta tagcatttac aatctgaggg cgtttttacg cctttttgga cagggatatt
    15601 gcaaggcata ttgttatttc tttttctttg ttttcttacc gtaaagctcg aggcggtgat
    15661 cgacgaggtc ataccctaag tctttggcga tttttatttt taatttttca agttcttcat
    15721 tttgaaattc aatgacttta ccggtttcaa gatcaaccaa atgatggtga tggtctgcat
    15781 ttacctcata acgggaatag ccttcctgaa actcatgccg aacgacaaga tccagttcat
    15841 ccaataagct taatgtgcga tacactgttg caatgctgat tgaagaatca agccccttgg
    15901 cacgatcata tacatcttca acggacgggt gatcatcagc ctctcctaaa acttttagaa
    15961 tcacgcggcg ctggcctgtc atttttaagc ccgcagacgc acatcgttct tccaaatccg
    16021 gcatttcaac ctctttgctt cttatcgtaa aaatcagtta agaccatacg atgggaataa
    16081 cgtgatgaca agatttggtt tgataccgct gtgtttttta ctgcttacat tcaagcgtcc
    16141 ggtaggctgg aaaagttaca aacacatctg ttcccttgcc aacttctgat tcgatatgaa
    16201 gtgttccatc atgcaaatca accatggcct tggccaaagg caggcctaga cctgtgcctt
    16261 caattgttga attttcctct ccgcttgtca cctgcccaaa cggttccatc gcctgttcaa
    16321 ttttgccatc gggaatgcct ttgccctgat cagaaacttt tatctgtatt cccttatcat
    16381 caagcatata ggctgatacg gttacggttt ttccagcctc agagaatttc atggcattcg
    16441 taaccaaatt gatcaggacc tggcgaatta agcgctgatc agctttaata ttgggaagat
    16501 ccttatcaac cttgatgacc aaacgaatgt tactgccaaa ggcccgagag tccatcatac
    16561 gtataaccga ctcgattaat cctccaacgt ccatttctcc ttcgtcaacc tcaattctac
    16621 ccgcttcaat tttggacatg tcgagaactt cgttaatgat agacagcagg tgccgcgcgc
    16681 ttgagtggac atcgccgata tactctttgt atttttcatg ccccaacgga ccgaatgttt
    16741 ccttgatcat catttctgaa aaaccgagga tcgcattcat cggtgtgcgg agttcatgac
    16801 tcatgtttgc taggaacaca gatttagcgc gattagcagc atcggcttgg tccttggcca
    16861 tgcgcaagga ttgttccatc tgtttacgca aggtgatgtc catcactgtt gtgacctgaa
    16921 agcgccgttt ctggcttagt tcgagagtcg ctgttgtata cagggcattg gcaatagaac
    16981 cgtctttgcg gataatcttc atttcacctg aagagcgcaa ccctgagcgg atatattctt
    17041 catggttacg ccgggcgagt tcgcgttcgt cgggcgtgat tatactgata acttcctggt
    17101 taaccagttc gtctcgtgac cagccgaacg ccctaataaa actatcattt acgcgtacaa
    17161 cgcgagcatt tacatctgtc acgacaatcc caacctcgct cacttcgaag attgaggtta
    17221 ggagtgaaat tgcatcgtcg cgctcgcgct ggacaattga ttgatctgca acgtcaaact
    17281 gcccgataac atcaagatat tctacattcc cctcttcgtc caaaattgga ctgatataaa
    17341 atccgtgaac acgtacagtt ccaggaacgc ttggcaaaga ctgaatggtt acagagcgtt
    17401 ttttagatat agcgacttcg aaagattgtt caaagtggat agcgttttcc gtattcatga
    17461 atttttttat gttttgccca acgacctgtt ttttatcaga tccaaaatag tcaagaccga
    17521 gttgatttgt ggcttcaacg cagtatgccc catcgcttgc ctgcttcact atgaagcgcg
    17581 gaatgcgcat ctcgttaaaa aattttaaat attctttgga catttcaacc ttagcattct
    17641 tgctttttaa gcgttctttt cagaatcact caaaatatag cgctgtagcg gactaagttc
    17701 ctgataggtc aggacagtgc cttgctcttt tatttgtgta tccaaatcaa catacagatt
    17761 gtctatcaga tcttcgatct tatccgctgg aatcggagat gcaacacagc aggacataga
    17821 tatattattt ctgtcattca aggcggccaa tgcctcactt ttctccagta actcccgcaa
    17881 acgctcaagc gctctttgac cttttgatat gtctgactgt ttaatgcaca taataaaatg
    17941 attgcggctc accctgtatg cctcgtcgta tgtacgcaat gttttttgga tcagctcggc
    18001 caccgttttc accagttcat cagctttttc ctgcccggct gttttttcaa attttgcaaa
    18061 gttatcaata cgcgccaagc cgacacagaa cggataacct tcgcgtgaaa gacgatccag
    18121 ttcaatttga agtccgctct tcattacaac attgtttttc aatcctgaga aaacatcaaa
    18181 gccccattct tccagcacga ttgtctgtgt gaaattctgc aattctatta tgtacgcgcg
    18241 aaaattatta taaaaccggc taaaattttc cttcgttata tccttttcac atgtgcttga
    18301 aagtgttagc agcccatgat gtgcttctaa taactgtctt cgccgcgtgt catagcgctc
    18361 gtcaatgtcg atagctttca gtgataggtc ctgaagccat tctacaaact tccggggaga
    18421 cgcagtggac tctgcctgtt cttttaaaaa aaaggacttt gccagtacgt tttggcacca
    18481 aaactggtac tcatccgtaa attcctggat aatcatgagt tgattattga gtgttttttg
    18541 aatagccata gtttttcatc atgttttgca aaaattggca tcgtgcctta tttggaagtt
    18601 ataccgtaat cgtattgatt tggttaataa aaagcagaaa aatttctttt ccaatgattt
    18661 agtgcgttcg ccactctttt tccagcttat cgagggtacc agaacggatg gcggcgcgaa
    18721 tttcacgcat caggcgattg atggtggcga cattatgctg ggcaagaatt tgcagcccca
    18781 gaatttcacc gcttttcagc aaatggtgaa tataggcttt ggagtaatcg catgaggcag
    18841 acacgccgtt tttttcatcc agaggcgttt tatcttcgcg ataacatgca tttttgaggt
    18901 taatcgtctc tccggggtgg cctttcatca gcgcgctgcc atggcgagcc aggcgggttg
    18961 ggataacgca atcaaacgta tcaacgccca tgcgcacgaa gcggaacaca tccttgatct
    19021 ggccgatccc gaggaaatgc acaggcctat ccgggtgcgc ataggggagg ctcgcagaaa
    19081 caacggtttc catttcttcg tcgctgccgc ccagacagcc gccgacggcg gtaccgaaaa
    19141 acggacggtc tttggtgtat tcggcggaga tgcggcgcag atcgctgtgc acgccgccct
    19201 gaacgatgcc gtagagacct tgagccccgt tatcatgacg ctcaaactcg gcaaggcagc
    19261 gatcgcccca gcgcatactc atttccatag agcgtgcagt gtagtcttta tcgacgtgat
    19321 acggggtgca ttcatcaaat tgcatgagca gatccgcgcc gagtttgcgc tggatttcca
    19381 tggcgctttc gggggtgagt ttgattttgc gcccatcaac gtaggagcgg aaaacacagc
    19441 cttcctcggt gattgagatc aggtttttat tctcgtggcc tttatcttga ttcttaccct
    19501 ttatttcgtt ggccatggtc ccttcgccca gcgaaaagac ctgaaagccc ccgctgtcgg
    19561 tgaggagcgg gccgttccag ttcatgaatt catgcagtcc gcctattttt tcgatcagat
    19621 cggcgccagg ctggagcatc aggtggtagg tgttggcgag aataatatcg gttttggcct
    19681 cgatcatttg cgccggggag agcgctttga tagcggcttt ggtgccgcaa aagatgtaat
    19741 tgggagtttc gataaccccg tgcggggttt taagcacccc cagccgcgcc ccggattttg
    19801 cgccccggtg ggtgatgtcg taagcgaaat tggggtaagc gatggtcatg atttttctaa
    19861 taaaaagccc gccagagctt tggcgggcat cttaacgttt taacgccgtt tacttaagca
    19921 gcttttgcac cctttgcctt ttcaatagcg gcttttaccg gacgcaatgc gggatcgagc
    19981 ttggttttgg ccgttacggt cagataagca tcaaagccgc ctttgtgctc aaccgtgcgc
    20041 agaccggcag agctggcctt tacttttacc catttattca aagcttcact gtacaggctt
    20101 gtatcctgaa cattggggag gaatttacgg cgcgttctat tcaaagcgtg agacacattg
    20161 ttcccgctca ttacgctctt tcctgtaatc atgcatttac ggctcatggc ctgattcctt
    20221 tatctcgtgt tcattcaggt gcggaaagta atcgaaagag cccctctaag tcaagctata
    20281 aaataccgcc ccacatatag gtggccaaaa gtacggcgac aaacaggata tgcgccatga
    20341 cattgatata gatcgcgcgg cgaaggtgtt tatgatcaag tttcgccgtt gctccttccg
    20401 ggccgaccca ttcgcccttt aacgctgttc ctgaaagatc ctgcacagcc ccaccgactg
    20461 aaacattcag cgcccaagac aaagctgaaa caggtgctcc gccttgcgcg tagggggcgc
    20521 ggtttttatg gccgaaccat gcggcaatgc ctttatgcag cctggctgtt ggagttagtc
    20581 ctgcggcgag cgtgatcagc agggccgcaa agaatgaggg gatcataccc attagccgct
    20641 ctaataccaa tggcacggcg gcaaaaccgc taccgaggcc attttttcca aaacgccagc
    20701 tgagagcagt gagcgccaca taggtgcaag cagctggaaa accgccgatt aaataccaca
    20761 gcgccggggc gacgagcccc ttatcaaagc ttcgggcact taagctcaca gcggcgcggg
    20821 tgacaccata atcatcgccg gcggcgagat tgatccgtgt ggtgcgggcg atggcgtaaa
    20881 aagccccttg cccgaccgta ttgttttcca ttgtaaaata gagttttaag agcacaaacc
    20941 agatgcttcc cgcggcaaat agcgaagaaa gtaatataat tcgcgtaata ccgtaagcag
    21001 gcgtttgcgc tgcaaatgtt tcgaagcttt ggccgatgaa ggtggctaga ataatcacga
    21061 gcacgcttaa aaacagcccg cgaaacatca aatcagcctg cgggcggtga gcgcggtcca
    21121 tccggtcgcc caacttgcca aaaaataaat ctataactct ccagaaaaac ggatgcgcgt
    21181 tacctgccaa aggcccggtg atcatgccga ctacggcgca gagcaacaac gccagcacaa
    21241 tgactggaat ccggtccaca ttcatgatct ggacatgcca ttgcgtgata aaattagtca
    21301 tcttcagtac gatttgcagt gtttttcccg tagaatgaag tgtacaataa tgcggttcac
    21361 ttatcaaagt ggaagctttc aaaaactttg acagaacttc aaaggtcacg gatatgctct
    21421 atcaacttta tgaattccaa catgccatga tgacgccgct gcgctttcag gcggagctta
    21481 tgcgcagcgc atttcaaaac ccttggaatc ctctgtctta tactcaggcc gggcgaatgg
    21541 tagcggcttc ggcggagatg attgagcgtt caacccggcg ctttggccgt cctgatttcg
    21601 gcttgcatga aacacagatt gatggacgca aggttgaggt agtagaaaaa aatatcgtgg
    21661 aaaagccgtt ttgccggttg cttaatttcc agcgcaaaat caagcgtcat gatccgaccg
    21721 tgctgctggt tgcgccgatg tccgggcatt atgcaacgct gttgcgcggg acagtggcag
    21781 ccctgctccc gcatcacaat gtttatatca ctgattggga ggatgcacgg caggttccgc
    21841 tgagcgatgg taagtttagc cttgatacgt acatcacata cctgcgtgaa tttatgagcc
    21901 tgttggggcc acaaacacac attatcggtg tgtgtcagcc ggtggtaccg gtgctggctg
    21961 ccgtatccct gatggcatcg gatgatgatc caaaccagcc gctgtcgatg actcttatgg
    22021 gcgggccgat tgatacgcgt gtttctaaaa ccgaagtgac ggaattggcg gaaacgcgcc
    22081 ccttaagctg gtttgaacaa agcgttgttc atgatgttcc attttactat cccggtgcac
    22141 accggagagt ttaccccggc tttctgcagc ttggcgggtt tatgtcgatg aatctggacc
    22201 ggcatgtggg ttcattcatg cagttttacc agcatcttgt tcaaggagac ggcgaaagcg
    22261 ccgtggcgca ccgtaagttt tatgacgagt acctttctgt aatggatatt ccggctgaat
    22321 tttatctaca aactgtagaa attgtttttc aacggcatct gttgccacgc ggagcaatga
    22381 agtggcgaga tcccttaagt gacgaattga tagatgtacg tccgcaagat attcgtaaaa
    22441 cggcgctttt aacaattgaa ggggaattgg acgatatttc ggcacgcgga caaaccacag
    22501 cggcgcatga gatttgctat tccatcccgc agcgcatgca atatcaccgt tttcagccgg
    22561 aatgcgggca ttatgggatt ttcaacgggc ggcgctggcg taacgaaatt atgccgcgtg
    22621 tacggcattt tattcgggaa tttgacccca gcgctgatcc ggtgccgaag aaagaccttg
    22681 agaaagtggc ggatctcagt gcttcacgct ataaccgtga tcggcacggc attgttgctg
    22741 tacggcgctg gattaaagaa caccgggcgg aaaattataa ggaaacaatt gattaaggcc
    22801 ttcagtgtca acgctcgggc atcgttaaat tttggcttac atagagtttg cctgcgtttc
    22861 gcgttattct ttctgcccga acacgaatac tgaaagtttg ttgtataaat tatttttttt
    22921 gtgttttttc tttgtttatc aatacttcca gacatttatc tggttttctt aaaatttaag
    22981 gattgaattt gaggctgcgt ctctctaata tccccttcat gcttttaaga caggaaacag
    23041 cgtttccgcc ttatgtcacg ctcaagcgct ccaaacgcgc gcggcggctg gcgttgcgcc
    23101 tcgaccccaa agaccggacc tttcatctgg tggttccggc ggggatgagt gtgaaaaaag
    23161 cccagcgctt cgccgaagag catgatcgct ggatgcagga taagctactc gaccttcctg
    23221 aaccaattcc gtttgaggat ggcagcattt tgccgatatt ggggcaaaac cgccgggtgc
    23281 atgtttttta tgattcgaca ttaagaacaa cctctattca tttgaaatac aatgaactta
    23341 tagttacaac aaacaaggaa gatccgacgg ccagaattga gcgcagctta aaaaaacggg
    23401 cgcgcgagga gttactggca ctttcccaac aaaaatcgga agcgattggt aagcgcgtta
    23461 aaggcgttgg tgcgcgcgac accaagagcc gctggggatc ttgcagctct gatggcaatc
    23521 tgtcgtattc atggcgattg atttttgcgc cttatgaagc gctggattat gtggtggcgc
    23581 atgaggttgc acatttaaaa tatatggacc attcgcgcgc attctggaac ctgtgccggg
    23641 agctgtccga taatttcatc gaggggcaat actggatgca ggaaaacggc catgagctga
    23701 tgcgctatgg gggatgattt tttaatcatt ctcaaatata caaaaatgca cgaaaccaag
    23761 cccttttaaa actaaaggca tgggtctgtg aacaggagga gcttttctcc taaagctgtt
    23821 ccctggtatt gtaactgttt ttcctctgaa tcggagccaa ctacttcaag aagccctcta
    23881 ctggctagat caaaaagacg tgcccttagt gatctaggtc tagcttcact cagccagacg
    23941 ccttcttttg ccctcatttc ttcaagggca ggaaaagaga ccgttgaaat tccctctcgg
    24001 acattattgg aagagcaagc agtaaaaata agctctatgt catatatgtc taaattagat
    24061 atatcttgtc gtagattact catttataaa ttttttccat ttttgctgta tattttccgt
    24121 aatatacagc agaatcaagc cctgtcaata ttttttcata aaatcaagcg cgccttggag
    24181 aatgacctgg gcggcgagct tgtcgatgag cccggaagcc tttgcattta cttttgtttt
    24241 tcgtttttgt accattttat ccacaatatt gtccgcagaa actgtggata aacgctcatc
    24301 ccacaatgca atccacggat cattgccaag cgttctttcc atctcaattg cgaaatcacg
    24361 aatgctttgc gcacacggac cttcagagcc atccatattg accggatagc ccaacacaaa
    24421 gccgtcaaca tcgtattcag ttatcacgac attcagaaac tccatatcct tggcaaatct
    24481 ggtgcgccgg atcgtttgca acggcgtagc cagcgtctgg ccgggatcag acagcgccaa
    24541 tccgatggtc tttttgccaa catcaaggcc gagcatcgcc acattcaaag gtcttctttc
    24601 aaccatatct tttaacaaac caacgctcac agcttaaaat cctttaaaat ccttattgtg
    24661 tctttgcctc ttcctgttta aaacagaaag cgcccaaata aacaatacaa ggtatagcaa
    24721 tgtctatgga tgaaaaaacc gttcgtaaag tcgcccggct ggcgaatatc gaaattgcgg
    24781 atcatgatgt tgagcggatt ggccatgagc tttccggcat tttggactgg gtggagcagc
    24841 ttggcgaggt ggataccgag aatgttgagc cgctggccaa cgttgtgaat attgagctcc
    24901 ccttgcgcaa agatgaagtt acagatggca actgctcgga gaaagtgctg gccaatgcgc
    24961 cggaagaaac gcaagggttc tttgtggtgc cgaaagtggt ggagcaagag ggataaaatg
    25021 agcaagctaa catatctcac tattgaagag gctctaaagg gtctgaataa taaagatttt
    25081 acggcagtgg agctggcgca agcgcatatt gatgcaatgg aggcaaactg cgatctgaat
    25141 gcgtttatca tggaaacgcc agagattgcg ctggcgcaag cggcagagag cgacaagcgt
    25201 tatgcggcaa gtgaagcgaa gccgctggac ggtattcctt ttgcgaacaa agacttgttc
    25261 tgcactgaag gggtgcaaac caccgcagcc agtaaaattc tggaaggttt tattccgcaa
    25321 tatgaatcca ctgtctcaag caagcttttt gcggatggcg cggtgatgct gggcaaggct
    25381 aacctcgatc agttcgcgat gggctcttct aacacaacca gcgcatttgg caatgtgatc
    25441 agtccatgga aggcaaccga tggtaaagat cttgtgccgg gcggctcgtc aggcggctct
    25501 gcggcggcgg tggcggcgcg catggcgatg ggcgcgacgg cgacagatac gggcggatct
    25561 attcgccagc ccgcgtcttt ttgcggtatt gtcgggatca agccgactta tgggcgctgc
    25621 tcacgttggg gggtggtggc gtttgcctct tcactggaca cgccaggccc cctcgcccgc
    25681 acggttaaag ataatgcact attgctggct tcaatggccg ggcatgatgc aaaagacagc
    25741 acatcggcca atcgcgatgt acctgacttt gccagtgcaa tcacgggcga tattagaggc
    25801 ctaaaaatag gtattccaaa agaatttaag ctggatggca tgccccccga aattgagaag
    25861 ctttgggaac aaggcatggc gtggctgaaa gaggctggcg ctgaactggt tgagatctcc
    25921 ctgccccaca caaaatacgc actaccgact tactatatca ttgctccggc tgaagcttca
    25981 tcgaatctgg cgcgttatga tggtgtacgg tacggccatc gttcaactga gagtaaaacg
    26041 ctggatgaca tgtataagaa aacccgcgct gaaggatttg gcgatgaagt taaacgtcga
    26101 attatgattg gcgcctatgt gctatccgcc gggtattacg atgcatatta catcaaggcg
    26161 cagaaggtcc gccgtctgat cttgcaagat tttgagaagg catatgaaac agttgatgcg
    26221 attttgacgc cgactgcgcc gtcgggggcg tttgccatcg gcgagaatga ggacgatccg
    26281 attaaaatgt atcttaacga tgtgttcacc gtcacagcta atctggctgg gattccggcg
    26341 atgtctgtgc ctgcggggct ggatgccaac ggcctg
//
LOCUS       DAOSWR010000009        25043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1273_length_25043_cov_5.146630, whole genome shotgun sequence.
ACCESSION   DAOSWR010000009 DAOSWR010000000
VERSION     DAOSWR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 25043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25043
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_25043_cov_5.146630"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PK513_01475"
     CDS             complement(<1..775)
                     /locus_tag="PK513_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOO81154.1"
                     /translation="MAKKKTPSKRKTTGKTTRKPASKSTGKATRKTTRKPARKTKAQK
                     TSRLRFAAKWLFVIGMWAGIALAGLFAYYAQELPAITEDASFERKRAIIVKAIDGSTV
                     ARYGEIVGNKVTVEDIPPYLVQAVLAIEDRRFYHHPGLDPLGLARAMFVNIKEGRVAQ
                     GGSTITQQLAKNLFLSQERTYKRKIQEAMLALWLEYELTKDEILSAYLNRVYLGSGVY
                     GVDAAARLYFKAPVKNLTLRECATLAGLLKAPSRYSPLRN"
     gene            complement(890..1423)
                     /locus_tag="PK513_01480"
     CDS             complement(890..1423)
                     /locus_tag="PK513_01480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81155.1"
                     /translation="MSASKLVKPAVEYKESFLEALDEFHAEGRYQFLDHAELEKDFET
                     FVDDLNEGSRHLHKTFSDWAEPVPETILWLVKEDKYIGTFNIRQRLNWHLEKWGGHIN
                     FVIRPSMRGKGFGKKILHKGMPYVSYLGIDRALITLNPRNKEGIRIVEFCGGEFEDET
                     PETDKFPSRLRYWLNCN"
     gene            1535..2752
                     /locus_tag="PK513_01485"
     CDS             1535..2752
                     /locus_tag="PK513_01485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOO81156.1"
                     /translation="MLSHFSNRIAVLDGLRAMAIVFVLLRHSVRPFWSDLSTPYIPIG
                     GLDLGAIFINGWVGVDLFFVLSGFLISSHLIRSFEKRRTLWSVLYNYAPRRFFRIAPV
                     YYLVLTLAVFGAFPYFPYPESYEGIGWRYAYHLVFMQDYWPSDIIAVFWSLAIEIKFY
                     LLAPFLIWGVLKLSDDRARIAVIGLLICSQPLARGLFAPEISGYEEYFLSIRTRFDWC
                     LDGLLMGMLAAFLLHSAAVRAVLLKPVVSGALFFFGVLLILGLSLPGPLVDLDVSRFD
                     IVWLAFLISAGFAAMLLGLLGECPGHGLFEKRGFSFIALISYSLYLVHLPLLYLAEVM
                     AERVVDFADMSERMGYVVYLPFFVVLCVFVSTLLYVFVERPFIVWSKKKYSRKAEEGE
                     EADDVGDGGQKDG"
     gene            complement(2672..4066)
                     /gene="mgtE"
                     /locus_tag="PK513_01490"
     CDS             complement(2672..4066)
                     /gene="mgtE"
                     /locus_tag="PK513_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO81157.1"
                     /translation="MQDEHDKTAENTTTEDVSFTLSDDEIRHALNALHAEDSDTIHAV
                     LEELSSADTAELLAKAPPEDRHEIIHMYGDTLEADVFSEMDTELRRTTLEQMPAAQVA
                     AIIAALESDDALDLIETFDENFQKEIIRKLSAKTRVALEEGLSFPEESAGRLMQREVV
                     AVPQFWTVGKTIDYLRAAASDLPEDFFDLFVIDPAYHVKGHVPLNKLIRARRAGKIED
                     LTMEDTHPVPADMDQEEVAHLFRRENIVSAPVVDNDNRLLGVITIDDVIDVIDEEAQE
                     DLLKLAGVDQGDLYRAVLSTTGTRFRWLFINLLTAILASLVISFFDATIQEIVALAVL
                     MPIVASMGGNAGTQALTVAVRALATKELSETNTARIIWKETIVGSLNGLAFAFITGII
                     AAAWFASPMLGLVIAMAMIINLFVAGIFGAAIPVLLDRSGADPAVASTVFLTTVTDVV
                     GFFAFLGLAAVFLL"
     gene            complement(4158..6563)
                     /locus_tag="PK513_01495"
     CDS             complement(4158..6563)
                     /locus_tag="PK513_01495"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOO81158.1"
                     /translation="MSKNKKNAPKDSKEALWKDALDYHRFPSPGKVALKPTTALSTQR
                     DLALAYSPGVAAPCEEIEKDPLTAYDYTSKGNLVAVITEGTAVLGLGNIGALAAKPVM
                     EGKSVLFKKFANIDSVDVEIDMNTQLEKDADGKYDREEYIQKFVNIVASLEPTYGGIN
                     LEDIKAPECFEIERRLKERMHIPVFHDDQHGTAIIVGAAFYNWLHIAKRDIKKIKLVT
                     SGAGASATACLNILIELGLPRENITVCDSKGVVYKGRSEYMDPVKETFAVETNARTLK
                     EAMEGADVFLGLSTAGVVKQDMVKGMGEKPLLMTLANPTPEIMPEEALEAKPDAIICT
                     GRSDYPNQVNNVLCFPFLFRGALDVGATAINEEMKLACVKAIAQLARKEATAEVAAVY
                     SDEQLEFGPEYMIPKPFDPRLIAELPKAVAQAAIDSGVATRPIKDWNAYSETLQSYTV
                     QTNMVMRPVISRARTAPKRVVYCEGEEENVLRAIQIVIDDNLAKPVVIGRRRVVERRL
                     EKFRLRMKIDKDFQLIDPENDPRYHEYWSDYHKIMQRRGVTPSSSRFTMRTNTTAIGA
                     LMVRRGEADAVICGITGQYHVHLKHITDILGKTEGVKTPSALMPLLLEKGPYFLCDTH
                     INDNPNTEELVEMAKMAAERVQRFGLTPKIALLSYSNFGNFNSPSAQKMREAAEILRA
                     EYEGTDIEVEGEMQADTALCQKTRDLLMPNSRLTGEANLLIGPNLDASNIAYNMLKVL
                     GNGIPIGPLLMGIAKPAHILTTASHSRRIVNVTALATVDAQVAAGEIHEHETIAPRKL
                     KSA"
     gene            complement(6612..6700)
                     /locus_tag="PK513_01500"
     tRNA            complement(6612..6700)
                     /locus_tag="PK513_01500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6664..6666),aa:Leu,seq:gag)
     gene            6799..7500
                     /gene="lipB"
                     /locus_tag="PK513_01505"
     CDS             6799..7500
                     /gene="lipB"
                     /locus_tag="PK513_01505"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010439342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOO81159.1"
                     /translation="MEWKISKKPVEYPEALDFMDARVADIHEGRADECVWLLEHPALY
                     TGGTSAKADDLLDAGGLPVYETGRGGEYTYHGPGQRVAYVMVDLKRRQKGPDIKRYVW
                     QLEEWIIQALAEFGIAGERREGRVGIWVKTPHPSFAGAQDTLSRGEREGPTQWEGEGI
                     IEEEKIAAIGVRVRRWVTLHGIAINVAPDLSHFSGIVPCGISEHGVTSMQALGVDASM
                     EEVDRVLREKFGEVF"
     gene            7601..7849
                     /locus_tag="PK513_01510"
     CDS             7601..7849
                     /locus_tag="PK513_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81160.1"
                     /translation="MPVPEMNFLQAAEDELGRLNDRGAFDEIWRAINLANHFDIQPSA
                     NGQVSRKEVGRVSEVLGIPCEGKECLGNRIYDAAARLG"
     gene            complement(7865..8743)
                     /locus_tag="PK513_01515"
     CDS             complement(7865..8743)
                     /locus_tag="PK513_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOO81161.1"
                     /translation="MKNIFSIEKPQNPLPLVFDSPHSGDRYPDDFDFACDFHTLESAE
                     DKYVEDLFAAAPNHGVTFLHAHFPRSYIDVNRAAEDIDPELLNGDWTGPHPIAPSSRS
                     HAGIGLIRRLVRPGVPVYDRNLTTEEILHRLDTYYHPYHNALEALINETHYNFGQVWH
                     INCHSMPASSAYPRRGIGLVGNRPKPADFVLGDRDATACDNDFTHALRDFLKELGYTV
                     SINDPFKGVELVEKYSSPAEGFHSLQFEINKSLYMDEETLEKTSDYNALKNHIEKLIQ
                     FCASYVESRITTLAAD"
     gene            8908..10179
                     /gene="serS"
                     /locus_tag="PK513_01520"
     CDS             8908..10179
                     /gene="serS"
                     /locus_tag="PK513_01520"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO81162.1"
                     /translation="MHDIKYIRENPNDFDAAMARRGLDGQSEAILKLDEERRGVQTEL
                     QRDQEMRNEISKQIGEIKKSGGDASQQMKDVAALKEGMSELEDKERALGEGLQNLLAC
                     LPNILATDVPDGADEDENVELRKWGEIKIPKGPDHAEIGEKLGMMDFETAAKMSGSRF
                     TLLSGGLARMERAIGMFFLDVHTSEHGYTEVSPPLMIRDNAMFGTGQLPKFKEDQFCT
                     TRGDWLLPTSEVPLTNIVAEMIVEEEYLPRRYTAFTPCFRQEAGAAGKDTRGMLRQHQ
                     FYKVEMVSVTAPEGSEAEHERMVGCAEAVLQKLEIPYRTIVLCSGDTGFGARKTYDIE
                     AWLPGQETYREISSVSNCWDFQARRMNARCRPKGEKQTRFVHTLNGSGVAVGRALIAV
                     IENYHDPGDGGVWVPEVLKPYMGGLEKIVKE"
     gene            10176..10577
                     /locus_tag="PK513_01525"
     CDS             10176..10577
                     /locus_tag="PK513_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81163.1"
                     /translation="MMTEVEVVLEMQKMHAEVVKDLGVFGLQALITINAGAVIALITF
                     IPQAIDSGKLKIDIKKIKAAVLLFLIGLFMAFLSILVTYMLAQMSFANFGVPSFWLFI
                     LLMVLPALLSFVCFAWGALKASQSIKENLLL"
     gene            10574..11254
                     /locus_tag="PK513_01530"
     CDS             10574..11254
                     /locus_tag="PK513_01530"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOO81164.1"
                     /translation="MNTQDINRRIRLVMDLRRKGIGDARVLGAMERVPRDEFVPAAVK
                     DQAWDDMALPIGRGQTISQPYVVAAMTQALQLSDRDKVLEIGTGCGYQSAILAKLCRR
                     VYTIERHKILLSRAEKMFEKLGLRNITVIAADGMLGWPKINGMSQAPFDKIIVTAAAR
                     EKPPQALLDQLKIGGAMIIPVGPSGDQWLKLYKKESEEAYSVRDVMPVRFVPLLPDIA
                     SDDEGQAA"
     gene            complement(11251..11463)
                     /locus_tag="PK513_01535"
     CDS             complement(11251..11463)
                     /locus_tag="PK513_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81165.1"
                     /translation="MTTEKTFDNYTIFTGIGIHIQFREAGATIETVNARDLEEELKIA
                     QTEGTQDPNYPLKRQALTECQAIRGF"
     gene            11573..12550
                     /locus_tag="PK513_01540"
     CDS             11573..12550
                     /locus_tag="PK513_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO81166.1"
                     /translation="MRYSVFGFFIVALSLSACLVQKPAPVSHYGTAEGAGSAGAYTVV
                     AGDNLWSISQRYDIVMRDIVVSNSLRAPFILNAGQRLKLPAPRTYRVRAGDSLYTVSR
                     LYNVSLSELAKLNRLSPPYVIQPGQVLRLPGVTPKTSPSVIRSARVSPRPEAEVLSLS
                     QEQGVPIPARKPSSGEVGVKVAKASNPKPVKVTTKPPKRSSSKFLKPVNGTVISSYGP
                     KKNGLHNDGVNIRAARGAGVKAADNGVVVYAGNEIKGSGNLILVRHDDRWMTAYAHLD
                     RIDVKRGDVLTRGQMIGTVGSTGSVDSPQLHFEVRRGTDAINPSRYLES"
     gene            12554..13018
                     /locus_tag="PK513_01545"
     CDS             12554..13018
                     /locus_tag="PK513_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81167.1"
                     /translation="MELLIMILAVICVPAGLYLLCGFLSMRSHGDIGEFEITGFQKAT
                     DKGRALPVVRYENGEGALKLVEVRKIEQISYLFSPPIERQMVEVAGIESGMPRVYGYV
                     RLVAGLFLLLPGLAALGLHLHKALFTGQVVYIVILIGLMLGGWALLKFIQRH"
     gene            13111..13524
                     /gene="yajC"
                     /locus_tag="PK513_01550"
     CDS             13111..13524
                     /gene="yajC"
                     /locus_tag="PK513_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOO81168.1"
                     /translation="MLISKAYAQSVEMSGNVPATLPNAPSATEAFIWNMGLVGLLVAM
                     FYILLIRPQQKRIKEHTSMLQGLKKGDKVVTAGGLIGRIAKIKDGDDEVQVELEGGMT
                     VTVLRSMLQAHGPRVSAGKPANDSKKDDKKKENKK"
     gene            13624..15174
                     /gene="secD"
                     /locus_tag="PK513_01555"
     CDS             13624..15174
                     /gene="secD"
                     /locus_tag="PK513_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOO81169.1"
                     /translation="MIQIAPWKIVLTLLVCALAFLYALPNAVSEQTRSWMQINLPGWA
                     PNKAVNLGLDLRGGAHLLYEVDVDLVLKERADSMLQDLRKSLREDKIGYTRIGVIPGG
                     VRLSLKNKKDGEGARRILRKLERNLDIDTSGDGLKIDAALDEAGKKALYDHTLAQSIE
                     IVRRRIDELGTTEPIIQRQSADRILIQAPGADAESLKAIIGTTAKLNFHLVKDPGTSG
                     ASKTYPLSEDPTQKVTVERRALITGDMLENAAPSFDQGGQPVISFRLNNTGARRFCDV
                     TRKNVNKPFAIVLDNEVLSAPVIREPICGGRGQISGGFSVQEANDLALLLRAGALPAP
                     MNVVEERTVGPSLGADSVEAGKKASLIGLALVLVFMALAYGGFGLMADVALIVNVALI
                     FAILSGLQATLTLPGIAGIVLTIGMAVDANVLIFERIREELRAGRSAISAIDAGYARA
                     MGTIIDSNLTTLIAALILFSLGTGPIKGFAVTLGVGIVTSFFTAIMVTRLMVVAWLKK
                     TKPSTVPV"
     gene            15337..16284
                     /gene="secF"
                     /locus_tag="PK513_01560"
     CDS             15337..16284
                     /gene="secF"
                     /locus_tag="PK513_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOO81170.1"
                     /translation="MQGLRLVPEETNFNFIGLRFLAYMVSAVLVFGSIALTVQRGLNF
                     GIDFTGGTVIEIRTPEAPDLAELRALLNDLDLGAITLQEFGSPEDLLIRLPEQKGGVE
                     AQQMAIEDVREALDDIYADAGEVDYRRTEFVGPQVGEELKKQGLLAVLFSLAGILAYV
                     WFRFEWQFGVASIIALAHDAIATIGLFALTQMEFNLSTVAAILMIAGYSINDTVIVFD
                     RIRENLRKFKKMSLPELFNKSVNEMLGRTLMTSGTTLLALIALYIFGGEVIQGFIYAL
                     IFGIVVGTYSSVFVAAPVLLNTGVKRGEVQSGDTTPQKA"
     gene            16290..16667
                     /locus_tag="PK513_01565"
     CDS             16290..16667
                     /locus_tag="PK513_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOO81171.1"
                     /translation="MDVTPLIRSGQQIIQSYSGGRFRVSGAVYEGALIVMPEGSQRWD
                     FAGEVADLSEPDFVPLIEQAAEIDVVLLGCGATIQFLSSNLKNALKACGLNVEVMDSG
                     AACRTYNVLMADGRRVAAALLPF"
     gene            16767..18569
                     /locus_tag="PK513_01570"
     CDS             16767..18569
                     /locus_tag="PK513_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81172.1"
                     /translation="MMAQSVSEQVRGLIESEGASAPEFGVREAEGQVAGWPDSLPMPV
                     ILEVGGRSGVGYITDENGQTMLLMHDPQAPGQVLAYDAEQMRRDYDAVATPLIEDLKA
                     TSPRDIYYGQDLGAGSSESLVRITSGDFDGEIRRKVVVGEGGNIERLGPLKLGEHRLG
                     KSAYLVDPTLRLGDRLPEDGRVFAAVVVHEDGSIKHHAPEHEAYRHSFADRPEVGALY
                     AQLSAYNSTIIYQNRGPGAEGGIAVKQKVSAEVLENLSIRVHERDAAVPSYEAVGSVV
                     QVAPEPGVPESQPVVPEVVVSEYYPSAFVQTAEQQSVDGGSAFIAQKIGNAESHMGNM
                     AALDREAFSQARVILPGLERELQKSPDLSDPAVQQQIEFMFNNDFGIADSYKAVAAQF
                     VEERLAAGGPIDMTPYNNALEALRDGDVSRAQGLLHGEVSESSVDVEQGRGAKVEEFR
                     GNAHSIEPVAGEVAPHFKNQEPSSVEGRENVALNSALESAVSDLQSAIATGENVDPAS
                     YEGMGLSSEAKTACMQYQELTGYKYSLDHSQQVLAGVGVNISDAIQLGADGRIQIHPD
                     KLPQEAAGLKDEFAAIKERVDAMPPANALEARCD"
     gene            complement(18625..19233)
                     /locus_tag="PK513_01575"
     CDS             complement(18625..19233)
                     /locus_tag="PK513_01575"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOO81173.1"
                     /translation="MSAFDLPELPYAYDALDPYMSAETLEFHHDKHHNAYIVNGNKIL
                     EGLDHMGNTLEEVVINAHKQGEQGLFNNAGQHWNHTHFWNWMKPNGGGARPSNLEAAI
                     TDSFGSFEAFREQFLNAGATQFGSGWCWLVMNAEGKLEVTKTANADNPLTSGQTALLG
                     CDVWEHSYYIDYRNARPKYLEAFVDNLVNWEYVAELFENASR"
     gene            19309..20097
                     /locus_tag="PK513_01580"
     CDS             19309..20097
                     /locus_tag="PK513_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOO81174.1"
                     /translation="MVKTISYCGRLVREHDPDRFLLSMFVPASVREGLWALFALNYEI
                     AKTREVVSETQLGLIRLQWWRERIAEIYDGKMPPGNEILESLAEAIARYDLPQEHFDT
                     LLHVREFDLEDVLPGNVEGLVNYADFTSTPLLKLAMQVVGGDVQREPVQVVAVNAGLV
                     GILRAVSFHARQRRCYLPEDLLKEAGQRLDHLYEFKPVEGLSDVIKSVLEQFVDGVSC
                     ENILLRKQQKLAEIYMWQLKSCDYDVFHSRMAVAPAFKVLRLIF"
     gene            20173..20445
                     /locus_tag="PK513_01585"
     CDS             20173..20445
                     /locus_tag="PK513_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81175.1"
                     /translation="MVDSIQGAGGPQNLSAANRGQNNKVSEDKRVEKTASQAPVRDEV
                     QISEEALSLSRDEQAAEQTAKETRTILQEQLDETLSADRQRVDKFL"
     gene            complement(20452..21117)
                     /locus_tag="PK513_01590"
     CDS             complement(20452..21117)
                     /locus_tag="PK513_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO81176.1"
                     /translation="MTEKPPKDIVVLLHGILRSKTDMLLLENFLEKNGYETINILYPS
                     REKSLEDLTDFVAEKIENHPGYSPKRPLNFVTHSMGGLITRYFIATHKPENLDKVVML
                     GPPNTGSEFADFLDDNEWLGPIFRSIFGPASGQLKTGYKHIDDDITYPLGIIAGSASV
                     NPLAPWILDGQHDGIVPVERTKIDGMSDHIVLPATHSFMMFNPKVMEQTLHFLQNGKF
                     KHA"
     gene            complement(21098..22516)
                     /gene="trmFO"
                     /locus_tag="PK513_01595"
     CDS             complement(21098..22516)
                     /gene="trmFO"
                     /locus_tag="PK513_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOO81177.1"
                     /translation="MSKTIHIIGAGLAGSEAAWQAANLGAHVILHEMRPDKGTPAHNT
                     GNCAELVCSNSFRSDDAEYNAVGLLHEEMRMLNSLIMREGEKAAVPAGGALAVDRDIF
                     SAGVTKALQDHPNITIQTGEITEIPPEWNNVIIATGPLTSDPLAAWIKEQTGEDELSF
                     FDALAPIVHFESINMDIAWMQSRYDKAEGIGTGKDYINCPMTKDQYETFVCELINAEY
                     GLFKEWELPSGAQTLAEAEIDTPYFDGCMPIEIMAARGPETLRFGPMKPVGLTNPHKG
                     ENEQPYAIVQLRQDNALGTLYNIVGFQTKMKWGEQTRIFKTIPGLENAQFARLGGLHR
                     NTFINSPKLLDAQLRLKFRPQIRFAGQITGCEGYVESASVGLMSGRMAASELLGIPFE
                     APPITTAHGALLGHITGGAKSETFQPMNINFGLFPVPENPFITMPNGKRKKLKGKDRK
                     KAYTTRALEDLENWMNNDRKAA"
     gene            complement(22533..22880)
                     /locus_tag="PK513_01600"
     CDS             complement(22533..22880)
                     /locus_tag="PK513_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81178.1"
                     /translation="MNNKKNTKIRRQAKQATMRGNYENAISLIDNGIKEQDRTFDDLL
                     TLQECLGLSGAWLQAECIRDILFDRINTVKQGKRFAALQERLDPPKLSNITRGRQFGG
                     AWHTRNNLDPSQG"
     gene            23022..23996
                     /locus_tag="PK513_01605"
     CDS             23022..23996
                     /locus_tag="PK513_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HOO81179.1"
                     /translation="MKKFLLSTVFSFVFVMPGWAEEAVVPVDAVQGKVQPVIEETADG
                     VVKVVQEAVVPAYAQEAGTDVQVVKAESSAGDRMSADEGHGDMDAETVAVEHHGTAQA
                     HWGYSGGEAAPYWGTLDGSYELCASGQEQSPVNIAKFLQEDVPDITLSYKPSSLNVVN
                     NGHTIQVNYDAGSGFEAAGVNYNLLQLHFHTPSEHYLDGAPYPMEMHLVHKAADGTLG
                     VIGVMMKIGGHNPVIEGVWQNAPIQAGGSKSIESVEINAADLMPASLEYYKYDGSLTT
                     PPCSEGVRWHVLKEPIEISEMQLKAFQALFPVNARPVQSLNDRVITGD"
     gene            23996..24502
                     /locus_tag="PK513_01610"
     CDS             23996..24502
                     /locus_tag="PK513_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81180.1"
                     /translation="MESLLAEIQYHASQAPDREMFALWRAVFSLAHGDGELAEVEQNL
                     VKQVMGVFSFSPEQCAVIEKDMHVPCDARHLFQEIELSEYRSQFFRLARIVVWCDGVL
                     HDDELAVIESIQDDLGDEAAVYEADLRWMNRKPDLPLGEQAASPEEEMLKQVIYQMIA
                     FYQQEGVG"
     gene            24499..24978
                     /locus_tag="PK513_01615"
     CDS             24499..24978
                     /locus_tag="PK513_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOO81181.1"
                     /translation="MSEAVTDSQFYMWRTLFAVAHADDVVSNEELRFMAEALDDIPFS
                     DEQRAILNEDVKTAQDIEKMFAGITDVRDQAHFFQFARELVHIDGDYSKEEQAVMLKL
                     EEAHLKCTNLDELIGSVGLEFEDDSQGMVFASAKRKKSFKDIVYSYRDEFLKNRFHK"
BASE COUNT         5259 a         4839 c         7971 g         6974 t
ORIGIN      
        1 ggttgcgcaa gggggagtag cgtgacggcg ccttgagcag gccggccagc gttgcgcatt
       61 cacgcaaggt aaggtttttg accggggcct tgaagtagag cctggcggcg gcatcaacgc
      121 cgtaaacgcc ggagccgaga tatacgcggt taagataggc ggagagaatt tcatccttgg
      181 tgagttcgta ttccagccac aaggcgagca tggcttcctg gattttgcgt ttataggtgc
      241 gttcctggga aagaaagagg tttttggcca gttgctgagt gatggttgag ccgccctggg
      301 cgactcggcc ttctttgata ttcacaaaca ttgcgcgggc aaggccgagc gggtcgaggc
      361 cggggtggtg gtaaaagcgg cggtcttcaa tggcgagcac ggcctggacg agatagggcg
      421 ggatgtcttc gacggtgact ttgttgccga caatttcgcc atagcgcgca acggtggagc
      481 cgtctatggc tttgacgatg atggcgcgtt tgcgttcaaa gctggcgtct tcggtgatgg
      541 cggggagttc ctgggcataa tatgcgaaca gtccggccag tgcgattccc gcccacattc
      601 cgattacgaa gagccatttg gcagcaaaac gcaggcgtga ggttttttgc gcttttgttt
      661 tgcgcgcggg tttacgcgtt gttttgcgcg tggctttgcc tgtgcttttg ctagcgggct
      721 tacgcgttgt tttgcctgtg gttttgcgtt ttgacggtgt cttcttttta gccatatgcc
      781 tttatacgga tatgaaaaaa acatgcaagt tttgtattac cggatctctg ctgtttaagc
      841 gttcgtcttc gctatgcttc agcgagacgc cgcagggatg acctgaaggt caattgcaat
      901 tcagccagta gcggagtcgg gacgggaatt tatcggtttc gggggtttcg tcttcgaatt
      961 caccgccgca gaattcaaca atgcggatac cttccttgtt tctcgggttg agtgtgatta
     1021 aagcccggtc gatgccaagg tagcttacgt aaggcatgcc tttgtgcagg atttttttgc
     1081 cgaaaccttt gccgcgcatt gaggggcgaa tgacaaaatt gatatgtccg ccccattttt
     1141 ccaaatgcca gttgaggcgt tggcggatat tgaaggtgcc gatatatttg tcttctttga
     1201 ctagccagag gattgtttcg gggacgggtt cggcccagtc agaaaaagtt ttgtggaggt
     1261 ggcggctgcc ttcattaagg tcgtccacaa aggtttcgaa gtctttttca agctctgcat
     1321 ggtcgaggaa ctggtagcgg ccttcggcgt ggaattcgtc taaagcttcg aggaagcttt
     1381 ctttatattc aacggctggt tttacaagtt tggatgcgga cataaaattt caggactatt
     1441 tttaaagatt attgggttaa cgttataaaa tcgctacact tcatgtgttg ggctattgtt
     1501 ttattcatca acgttaaaga gtgtatttgg gtctgtgctt tctcactttt caaatcgaat
     1561 tgctgttttg gatggcctgc gggccatggc aattgttttt gttttactcc gtcactcagt
     1621 cagacccttc tggtctgatt taagcactcc ttatatacca attggcgggc ttgatctagg
     1681 ggcgattttt atcaatggct gggtgggtgt ggatcttttt tttgtgctta gcgggttttt
     1741 gattagttcg catttgatcc gttcatttga aaagcgccgg acgctttggt cggtgctgta
     1801 taattatgcg ccgcgccggt tctttcggat tgcgccggtg tattaccttg tgctgacgct
     1861 tgcggttttc ggagcgtttc cctatttccc ttatccggag agttatgagg ggataggctg
     1921 gcgttatgcg tatcatcttg tgtttatgca agattactgg ccttcggata ttattgcggt
     1981 tttctggtcg cttgcgattg agatcaagtt ttacctgctg gcgccgtttt tgatttgggg
     2041 ggtgttgaag ctctcagatg atcgggcgcg cattgcggtt ataggtttgt tgatttgttc
     2101 ccagcctttg gcgcggggtt tgtttgcgcc ggagattagc ggatatgaag agtatttttt
     2161 atctattcgt acgcggtttg attggtgttt ggacgggttg ttgatgggca tgctggcggc
     2221 attcttgttg catagcgcag cggtgcgggc agttttatta aaacctgttg tttcgggcgc
     2281 gttgtttttt tttggggtgc ttttgatttt agggctgagt ttgcccggcc cactggtgga
     2341 tctggatgta agcaggtttg atattgtctg gttggccttt ttgatctcgg ctggttttgc
     2401 ggccatgctt ttggggttgc tgggtgagtg cccggggcat ggtttatttg aaaagcgcgg
     2461 ttttagtttt attgcgctga tttcttatag tttatatctg gttcatttac cgcttcttta
     2521 tctggctgaa gtgatggcag agagggttgt ggattttgca gatatgagtg aacggatggg
     2581 atatgtggtg tatttaccgt tttttgtcgt gctgtgtgtg tttgtttcga cgctgcttta
     2641 tgtgtttgta gagcgaccat ttatagtttg gtcaaagaag aaatacagcc gcaaggccga
     2701 ggaaggcgaa gaagccgacg acgtcggtga cggtggtcag aaagacggtt gatgcgacag
     2761 ccgggtctgc cccggagcga tccagaagga cggggatggc tgcgccgaaa attccggcga
     2821 caaacaggtt gatgatcatg gccatggcga tgacgaggcc gagcatgggg cttgcaaacc
     2881 aggcggcggc gattatgccg gtgataaatg cgaaggcgag gccgttgagc gagccgacga
     2941 ttgtttcttt ccagatgatg cgcgcggtgt tggtttcgga cagttctttg gtggccaaag
     3001 cgcgcacggc gacggtcagg gcttgcgtgc ctgcgtttcc gcccatggac gcaacgatgg
     3061 gcatgagcac agcgagggcg acgatttcct gaattgtggc atcaaagaac gaaattacga
     3121 gtgaagcgag aatggcggtg agcaggttta tgaacagcca acgaaagcgt gtgccagtgg
     3181 tggaaagtac ggcgcggtaa aggtcgcctt gatcgacccc ggcgagtttg agcaagtctt
     3241 cctgcgcttc ttcgtcaata acgtcgatga cgtcgtcaat ggtgataacg ccgagcagac
     3301 ggttgtcgtt atccaccacg ggtgctgaga caatgttttc gcgtcggaaa aggtgggcga
     3361 cttcttcctg atccatgtcg gcgggaacgg ggtgggtgtc ttccattgtc aggtcttcga
     3421 ttttgccagc acggcgtgcc cgaatgagtt tgtttagcgg gacatggcct ttgacgtggt
     3481 aggcggggtc gatgacgaaa aggtcgaaga agtcttcggg caggtcggag gcggcggcgc
     3541 gcagatagtc tatggttttg ccaacggtcc agaattgcgg cacagcgacg acttcgcgtt
     3601 gcatcaggcg tccggcactt tcttcaggga aggagagacc ttcttcgagg gcgacacgag
     3661 ttttggccga gagtttgcgg atgatttctt tttgaaaatt ctcgtcgaat gtttcaataa
     3721 ggtcgagtgc gtcatcgctt tccagagccg caataatcgc ggcgacttgt gcggcgggca
     3781 tttgttctag ggtggtgcgg cgaagctctg tatccatttc agaaaagaca tccgcctcca
     3841 gcgtgtcgcc gtacatatga atgatttcat gacggtcttc ggggggcgct ttggcgagaa
     3901 gctcggcggt atcggcagag cttagttctt ccagaaccgc gtggatggta tcggagtctt
     3961 cagcgtgcag ggcgtttagc gcgtggcgga tttcgtcgtc gctgagtgta aagctgacgt
     4021 cttcggtggt tgtgttttca gccgttttgt cgtgttcatc ttgcatgcgg gctggtcctt
     4081 ggttgagtag ataaaggctc tctaacatcc ccgaaaaggg ggtgttagat caagtggatt
     4141 ttatgcgcag agccttttta ggcgcttttg agtttgcgcg gggcgatggt ttcgtgctcg
     4201 tgtatctcgc cggcggcgac ttgcgcatcg acggtcgcca gtgcggtgac attgacaatg
     4261 cggcggctat ggctggcggt tgtcaggatg tgcgccggtt ttgcgattcc cattaaaagc
     4321 gggccgattg gaatgccgtt gcctaaaact ttcagcatgt tgtatgcgat gttggaggcg
     4381 tcgaggttgg ggccgatcag gaggtttgct tcgccggtga gacgggaatt gggcattaag
     4441 aggtctcgtg ttttttggca cagagcggtg tcggcttgca tttcgccttc gacttcgatg
     4501 tctgtgccct catattcggc gcgcagaatc tcggcggctt cgcgcatttt ttgcgccgac
     4561 gggctgttga agtttccgaa gttggaatag gacagcaagg caattttggg cgttagaccg
     4621 aaacgctgga cgcgctcggc ggccattttg gccatttcga caagctcttc cgtattcggg
     4681 ttgtcgttga tatgggtgtc acacaggaaa tacgggcctt tttcgaggag caggggcatc
     4741 aatgcggaag gggttttgac gccctccgtt ttgccgagaa tgtccgtgat gtgtttgagg
     4801 tgaacgtgat attggccggt gatgccgcag attacggcat cggcttcgcc gcgccggacc
     4861 atgagcgcgc caatggcggt tgtgtttgtg cgcatggtga aacgggagct tgagggcgtt
     4921 acgccgcggc gctgcatgat tttgtgataa tcgctccagt attcgtggta gcgcggatcg
     4981 ttttccggat cgatcagttg aaagtctttg tcaattttca tgcgcaggcg gaatttttca
     5041 aggcgtcttt ccacgacgcg gcggcgacca atgactacag gtttggcaag attgtcgtca
     5101 ataacgatct ggatggcgcg taggacgttt tcttcctcac cttcgcaata gacaacgcgt
     5161 ttgggtgcgg tgcgggcgcg agagatgacc ggacgcataa ccatgttggt ttgtacggta
     5221 tagctttgaa gtgtttcgct gtaggcattc caatccttga tagggcgcgt ggcgacgccg
     5281 ctatctattg cggcttgtgc cacagctttg ggaagttcgg caatcagacg cggatcgaac
     5341 ggtttgggga tcatatattc cgggccaaat tcgagttgtt cgtcgctgta gactgcggcg
     5401 acttcggcgg tggcttcctt acgggctagc tgggcgatgg ctttaacgca agcgagcttc
     5461 atttcctcgt taatggctgt ggcgccgaca tcgagcgcgc cgcggaacag gaatgggaag
     5521 cacagcacat tgttaacctg gttggggtag tcagaacggc cagtgcagat aatggcgtcc
     5581 ggtttggctt ccagagcctc ttcaggcatg atttccgggg ttgggttggc cagtgtcatg
     5641 agcaggggtt tttcacccat gcctttgacc atgtcttgtt taacgacgcc ggcggttgac
     5701 aggccgagga agacgtcggc gccttccatg gcttctttga gggtgcgggc gttggtttca
     5761 acggcgaagg tttctttgac cgggtccata tattccgagc ggcctttata aacgacgccc
     5821 ttactgtcgc atacggtgat gttttcgcgc ggcagaccga gttcgatgag gatgttgagg
     5881 caagcggtgg ccgatgcgcc cgcgccggag gtgacgagtt ttattttctt gatatcgcgt
     5941 tttgcaatat gcaaccaatt atagaaggca gcgccgacga taatggcggt gccgtgctgg
     6001 tcgtcatgga agacgggaat gtgcatgcgc tctttcaagc ggcgttcgat ttcgaaacat
     6061 tcgggcgctt tgatatcttc gaggttgatg ccgccatagg ttggttcgag tgaggcgaca
     6121 atgttgacga atttctggat atattcttcg cggtcatatt taccatcagc atctttttcc
     6181 agttgtgtgt tcatgtcgat ttcaacatcg acggaatcga tgttggcgaa ttttttgaac
     6241 aggaccgatt taccttccat cacgggtttg gcggccaatg cgccgatatt gccgaggcca
     6301 agtacggcgg tgccttcggt gattacggcc acgaggttgc ctttggaagt gtaatcgtag
     6361 gccgttagcg ggtctttttc gatttcttca cacggggcag cgacgccggg ggaataagcc
     6421 agagctagat cgcgctgggt tgaaagcgcg gttgtgggtt tgagcgcgac tttaccgggt
     6481 gaggggaaac ggtggtaatc cagcgcgtct ttccagagag cttctttact gtctttgggg
     6541 gcattctttt tatttttgct catagcgtct ggctgctttc gatcattttt gttgctatca
     6601 gttttttaag atggtgcggc tgagaggact tgaacctcca cgggatttaa ctcccacagc
     6661 gacctcaacg ctgcgcgtct accaattccg ccacagccgc atatcttaat gaggtgcgct
     6721 ttgatagcaa aagcaagggt ggagtgcaag tgtgtatgag tgttgcagtg cgatagagtt
     6781 tcctttattg aagcttttat ggaatggaag atttcgaaaa agccggtgga atatcctgag
     6841 gcgttggact ttatggatgc gcgggtggcg gatattcatg aaggccgggc cgatgagtgc
     6901 gtgtggctgc tggagcatcc ggcgctgtat accggtggga caagcgcgaa ggcggatgat
     6961 ttattggatg ctggcggttt gccggtttat gagacggggc gcggcgggga gtatacctat
     7021 cacgggccgg gccagcgggt ggcttatgtg atggtcgatt tgaagcggcg gcagaaggga
     7081 ccggatatca agagatatgt ctggcagctg gaagagtgga ttattcaggc attggcggaa
     7141 tttggaattg ccggggagcg gcgggaaggg cgcgtgggaa tttgggtaaa aacccctcac
     7201 ccgtctttcg ctggcgctca agacaccctc tcccgtgggg agagggaggg gcccacgcag
     7261 tgggagggtg aggggattat tgaggaggag aagattgcgg cgatcggggt gcgggtgcgg
     7321 cgctgggtaa cgcttcacgg aattgcgatt aatgttgcgc cggatttatc gcatttttcg
     7381 gggattgtgc catgtggaat tagcgagcac ggggtgacga gcatgcaggc gctgggcgtg
     7441 gatgcgtcga tggaagaagt tgaccgggtt ttgcgggaga aattcgggga ggttttttaa
     7501 cgcggagata cggaggttcg gagaatggtg ttgacatgga aagtttttgt agcctataag
     7561 caatattatt cataataaca gtatttttaa ggagtagagt atgccggtac cggaaatgaa
     7621 ttttttacaa gccgctgaag atgagttggg tcgattgaat gatcgaggcg cttttgatga
     7681 aatttggcgg gctataaatt tagcaaacca ttttgatatt cagccaagcg caaatggaca
     7741 ggtttcccgg aaagaagtgg gccgagttag tgaagtgctt ggtattcctt gtgaaggtaa
     7801 agagtgtctt ggaaaccgta tctatgatgc ggctgctcga ttggggtagg cgtaataacg
     7861 tgttctaatc cgccgcgagg gtggtgatgc ggctttcgac gtaggaagcg caaaactgaa
     7921 tcagtttctc aatatggttt ttgagcgcgt tgtaatcgct ggttttttcc agcgtttctt
     7981 catccatata aagggatttg ttgatttcaa actggaggga gtggaagcct tcggcggggg
     8041 aggagtattt ctcgaccagt tcgacgcctt tgaaggggtc gttgatggac acggtgtagc
     8101 ctaattcttt gagaaaatcg cgcagggcgt gggtgaagtc attgtcgcag gcggtggcat
     8161 cgcgatcgcc aaggacgaaa tcagcgggct ttgggcggtt gccgacaaga ccgattccac
     8221 ggcgcgggta ggcgctggat gcgggcatag aatggcagtt gatgtgccag acctggccga
     8281 aattgtagtg ggtttcgttg atgagcgctt cgagtgcatt atgataaggg tggtaatagg
     8341 tatcgaggcg gtggagaatt tcttctgttg tcaggttgcg gtcataaaca ggtacgccgg
     8401 gccgcacgag gcggcggatg aggcctatgc ccgcgtgaga gcgggacgag ggggcgattg
     8461 gatgtggacc ggtccagtcg ccgttcaaca gttccgggtc gatatcttcg gcggcgcggt
     8521 tgacgtcgat atagctgcgc gggaaatggg cgtgcaaaaa ggtgacgccg tgattgggcg
     8581 cggcggcgaa gaggtcttcg acgtatttat cctcggcgga ttcaagcgtg tgaaaatcgc
     8641 aggcaaagtc gaaatcatcg gggtagcggt cgccgctgtg ggggctgtca aaaaccagcg
     8701 gtaggggatt ttggggtttt tcgatggaga aaatgttctt catacttatt tttaacgcga
     8761 agacacgaag aagcgaagtt ttttaatgcg tagaaaaatt atatttgtca ttgcgagcct
     8821 tggcgaagca atctacattt cagtcaaatt gaatggattg cttcgtcgct atgctcctcg
     8881 caatgacgat ttatggatta ggtaaaaatg cacgacataa aatatatacg cgaaaatccg
     8941 aatgactttg acgcggcgat ggcgcggcgc gggctggatg gacagtctga agctattttg
     9001 aagctggatg aagagcggcg tggggtgcag actgagttgc agcgtgatca ggagatgcgt
     9061 aatgaaattt ccaagcagat tggcgagatt aagaaatccg gtggtgatgc gtctcaacaa
     9121 atgaaagatg tggcggcgtt aaaagagggg atgagtgagc ttgaggataa agagcgggcg
     9181 ctgggtgagg gtttgcagaa tttactggcc tgtttgccga atatactggc gacggacgtt
     9241 cctgatgggg cggatgagga tgagaatgtc gagctgcgta aatggggcga gattaaaatc
     9301 ccaaaaggtc ccgatcatgc cgagattggc gaaaagctag ggatgatgga ttttgaaacg
     9361 gcggcgaaaa tgtcggggag ccggtttacg cttttgagcg gcgggttggc gcggatggag
     9421 cgggcgatcg ggatgttttt cctcgatgtg catacgtccg agcatggtta tacagaggtg
     9481 tctccgcctt tgatgattcg tgataatgcc atgttcggca ccgggcagct tccgaaattt
     9541 aaagaggatc agttttgtac gacacgcggg gattggttgt tgccgacttc ggaagtgccg
     9601 ttgaccaata tcgttgccga gatgattgtg gaagaagagt atctgccgcg tcgctatacg
     9661 gcctttacgc cgtgttttcg gcaggaagcc ggggcggcgg ggaaggatac gcgcgggatg
     9721 ttgcgtcagc accaattcta taaagttgag atggtgagcg tgaccgcgcc ggaaggcagc
     9781 gaggccgagc atgaacgtat ggttggctgc gcggaagcgg ttttgcagaa gctggagatt
     9841 ccgtatcgca cgattgtgtt atgtagcggg gataccgggt ttggggcgcg taagacctat
     9901 gacattgagg catggttgcc ggggcaagaa acgtaccgcg agatttcatc ggtatcgaat
     9961 tgctgggatt ttcaggcgcg gcggatgaat gcgcgttgcc gtccgaaggg agagaagcag
    10021 acgcggtttg tgcatacgtt gaacgggtcc ggtgtggcgg tgggccgggc gttgatcgcg
    10081 gtgattgaga attatcatga tccgggcgat gggggggttt gggtgccgga ggtcttgaag
    10141 ccgtatatgg gtggattaga gaagattgtg aaggaatgat gactgaggtt gaggtagtct
    10201 tggaaatgca aaaaatgcat gcagaggtcg taaaagacct tggcgttttt ggtttgcagg
    10261 cacttataac aataaatgca ggggcggtta ttgctctaat aactttcata ccgcaagcga
    10321 tcgattctgg aaaattaaaa attgatatta agaaaattaa agcggctgtt ttgttgtttc
    10381 tgattgggtt atttatggct tttctttcta ttttggttac gtatatgctc gctcaaatga
    10441 gttttgcaaa ttttggtgtg ccaagctttt ggttatttat tttgctgatg gttttacccg
    10501 cattactttc ttttgtttgt tttgcttggg gcgcgttaaa ggcatctcag tcaattaaag
    10561 aaaatttatt gctatgaaca cgcaggatat aaatcgtcgg atacggttgg ttatggatct
    10621 tcgcaggaag gggattggcg acgcgcgtgt gctgggggcg atggagcggg tgccacgtga
    10681 tgagtttgta ccggcggcgg tgaaggatca ggcgtgggat gatatggcct tgccgatcgg
    10741 acgcgggcag acgatttccc agccttatgt ggtggcggcg atgacgcagg ctttgcagct
    10801 ttcagaccgg gataaggtgc tggagattgg cacggggtgc gggtatcaga gcgcgatttt
    10861 ggcgaagctg tgccggcggg tttatacgat tgagcggcat aagattttgc taagccgcgc
    10921 ggagaaaatg tttgaaaagc tgggtttgcg caatattacc gtgattgcag cggacgggat
    10981 gcttggttgg ccgaagatta acgggatgag ccaagcgcca tttgacaaga ttatcgtgac
    11041 ggcggcagcg cgggaaaagc cgccgcaggc gttgctcgat cagcttaaaa tcggcggggc
    11101 gatgattatt ccggtggggc cgagcgggga ccaatggctc aagctgtata agaaagagag
    11161 cgaggaggcc tatagcgtgc gcgatgttat gccggtgcgg tttgtgccgt tattgccgga
    11221 tatagcgagc gatgatgagg ggcaggcggc ttagaagcca cggatggctt ggcattcagt
    11281 gagcgcttgt ctttttaggg ggtagtttgg gtcttgcgtg ccttcggttt gcgcaatttt
    11341 taattcctct tctaagtcgc gagcgtttac cgtttctata gttgcgccag cttcacggaa
    11401 ttgtatatgg atgcctatgc ctgtgaaaat tgtataattg tcaaaagttt tttctgttgt
    11461 cattttttgt ctccttagcc ggtgatttat tatgtaattt cgtgtagttt gtcaagaaaa
    11521 ttaggcattc ctatttcttt tgatgtccta atttgtgcgt tatactgctc tgatgagata
    11581 ttctgtcttt ggttttttca tagttgcttt gtccttgtcc gcctgtcttg ttcaaaagcc
    11641 agcgccggtg tcgcattatg gcacggcgga aggggcgggg agcgcggggg cctacacggt
    11701 agtggccggc gataatctgt ggagcatttc acagcgttat gacatcgtga tgcgcgatat
    11761 tgtggtgagc aacagtttgc gagcgccgtt tattcttaat gctgggcagc ggttgaagtt
    11821 gcctgcgccg cggacttacc gtgtgcgcgc tggggatagt ctttatactg tgtcgcgact
    11881 ttataacgtg agtttgtccg agcttgccaa gcttaaccgg ttaagtccgc cttatgtgat
    11941 tcaaccgggg caggttttgc gcttgcccgg tgtgacgccg aagacgagtc cgtccgttat
    12001 acgcagtgcg cgcgtgtcgc cgcggccgga ggcggaggtt ttgtcacttt cgcaagagca
    12061 gggggttcca attcccgcac gcaaaccttc ttccggtgag gtaggggtta aggttgcgaa
    12121 agcgtcaaat cccaagcctg ttaaggtgac gaccaagccg ccgaagcgtt catcgtcgaa
    12181 atttttgaag ccggtgaatg gaacagtcat ttcatcttat gggccgaaga aaaacgggtt
    12241 gcataatgac ggtgttaata tcagagcggc gcgcggggcc ggggtgaaag cggcggataa
    12301 cggcgttgtg gtgtatgcgg gaaatgagat caaaggttcg gggaatttga ttctggtgcg
    12361 ccatgatgat cgctggatga cggcctatgc gcatctggac cggatagatg tgaagcgcgg
    12421 agatgtttta acgcgcggcc agatgatcgg cactgtcggt tcgactggtt ccgtcgatag
    12481 tccgcaattg catttcgagg tgcggcgggg aacggatgcg attaatcctt ctcgatattt
    12541 ggaaagctag gttatggaat tattgattat gattttggcg gttatttgtg ttccggcggg
    12601 actgtatttg ctatgcgggt ttttgagtat gcgttcgcac ggcgacattg gggaattcga
    12661 gattacaggg ttccagaaag ctacggataa ggggcgtgca ttgccggttg tgcgttatga
    12721 gaacggggaa ggggctctta aacttgttga ggtgcgcaag attgagcaaa tttcttattt
    12781 gtttagtccc ccgattgagc ggcagatggt tgaggttgct ggcatagaga gcggtatgcc
    12841 gagagtctat ggttatgtgc ggttggtggc gggactgttt ttgctgttgc ccggcctggc
    12901 ggcgctgggg ttgcatttgc ataaggcgtt atttaccggg caggttgttt atatcgtgat
    12961 tttgattggt cttatgcttg gcggatgggc tttgctcaaa tttatccaac ggcattaaca
    13021 gcataaaggg tgagacgctt gctttcggcg cgccaatctt tatagtcctc tcattaacag
    13081 aatttgagtc gaaatataag gagcatattg atgctgattt ccaaagcgta tgcacaaagt
    13141 gttgaaatga gcggaaatgt cccggcgaca ttgccgaacg cgccaagcgc gacagaggcc
    13201 tttatctgga atatggggct ggtcgggttg cttgtggcta tgttttacat tttgctgatc
    13261 cgtccgcagc aaaagcgaat taaagaacat acgagtatgt tgcaagggct taagaagggc
    13321 gacaaggttg taacggccgg tgggttgatt ggcagaattg ccaagatcaa agatggtgat
    13381 gatgaggttc aggttgagct ggagggcggt atgactgtga cggttttacg ttctatgcta
    13441 caggcgcacg gaccgagagt gtctgcgggg aagcctgcga acgactctaa aaaagatgat
    13501 aagaaaaaag aaaataagaa gtaagaaagg cgtcattgcg aggagagccg tttggctcga
    13561 cgcggcaatc cagtttgtga ttctagattg cttcgctgcg ctcgcaatga cggatgacga
    13621 gacatgattc aaattgcacc ctggaaaatt gttctgacgc tgctcgtttg cgcgctggcg
    13681 tttttgtatg ccttaccaaa tgctgtgagt gagcagacgc ggagctggat gcagattaat
    13741 ttgccgggct gggcgccgaa taaagcggtt aatctggggc tggatttacg cggcggggcg
    13801 catttgcttt atgaggttga tgttgatctg gtgctcaaag agcgggcaga ttctatgctt
    13861 caggatttgc gcaagtcttt gcgcgaggac aaaattggtt atacgcgcat tggcgttatt
    13921 cctggcggtg tgcgtcttag tttgaaaaat aagaaagatg gtgaaggcgc gcggcggata
    13981 ttacgcaagc tggagcgtaa tctggatatt gatacttccg gtgatggcct gaagattgat
    14041 gcggctcttg atgaggcagg caagaaggcg ctgtatgacc atacattagc gcagtcgatt
    14101 gaaattgtgc ggcggcggat tgatgagttg ggtacaacgg agccgattat tcagcgccag
    14161 agcgctgacc ggattttaat tcaggcgccg ggcgcggatg cagaaagttt gaaggcgatt
    14221 attgggacaa cggcgaagct gaattttcat ttggttaaag atccagggac ttcgggggcg
    14281 agtaaaactt atccgctgtc ggaagatcca acgcagaaag tgacggtgga acgccgggcg
    14341 ttgattaccg gggatatgct ggaaaacgca gcgccgagct ttgatcaggg cgggcagccg
    14401 gtgatttctt ttcgtttgaa taatacgggc gcgcggcgct tttgcgatgt gacacgcaag
    14461 aatgtgaaca agccgtttgc gattgtgttg gataacgagg ttttgtccgc gccggtgatc
    14521 cgcgagccga tttgcggtgg ccggggccag atttcgggcg gttttagtgt gcaagaagct
    14581 aacgatctgg cacttttatt gcgcgccggg gctttgcctg cgccgatgaa tgtggtggaa
    14641 gagcgcacgg ttgggccttc tctgggggcg gatagcgttg aagccgggaa gaaggcgagc
    14701 ctgattggtt tggcgctggt tctggtgttt atggcgctgg cttatggcgg gtttggcttg
    14761 atggcggatg tggcgttgat tgtgaatgtg gcgctgattt ttgcgatttt gtcgggcttg
    14821 caggcgacgc tgaccttgcc ggggattgcc gggattgtcc tgaccatcgg gatggcggtg
    14881 gatgcgaatg ttttgatttt tgagcgtatt cgcgaggagc tgcgcgccgg gcgcagtgcg
    14941 atatcggcaa ttgatgcggg atacgcccgc gcgatgggca ctattattga ttctaacctg
    15001 acaacgttga ttgcggcgtt gattctgttt tctttgggga cggggccgat taaaggtttt
    15061 gcggtgacgt tgggtgtggg aattgtcacg tcgttcttta cggcgattat ggtgacgcgt
    15121 ttgatggtgg tggcttggtt gaagaaaaca aaaccgagca ccgtgccggt ttaaaatcgc
    15181 ccataatggg ctgcaaatag tagttttcta cgcttccgtt actcacgtac caaaatgtac
    15241 gctgcgctac ggttctcgaa aaccactgtt ttcgccgcat tctggatgat ttttgagtat
    15301 gtacagattt gaatgaataa aattaaggaa gtttgaatgc aaggtttacg attagttccg
    15361 gaagaaacga attttaattt tataggcttg cggtttttgg cctatatggt ttcggctgtt
    15421 ttggtgtttg gttcgattgc tttaactgtt caaagaggtt tgaatttcgg gatcgatttt
    15481 accggcggga ccgtgattga aatccgcacg cctgaggcgc ctgatttggc agagcttcgc
    15541 gctttgctta atgatctgga tctgggtgca attacattgc aggaatttgg ttctccggaa
    15601 gatttgttga tccgtttgcc agagcaaaag ggcggcgttg aagcacaaca aatggcgatt
    15661 gaagatgtgc gtgaggcgtt ggatgacatt tatgcggatg cgggtgaagt ggactatcgc
    15721 cgcactgaat ttgttggacc gcaggttggt gaagaactta agaagcaggg tttgctggca
    15781 gtgctgtttt ctttggctgg tattcttgcc tatgtatggt ttcggtttga gtggcagttt
    15841 ggcgtggctt ccattattgc gctggcgcat gatgcgattg caacgatcgg gttgtttgcg
    15901 ttgacgcaga tggagtttaa cctttcaacg gtggcggcga ttttgatgat cgcgggatat
    15961 tctatcaatg atacggtgat tgtgtttgac cgtattcgtg agaatttgcg caagtttaag
    16021 aaaatgtcct tgcctgagct gtttaacaag tccgttaatg agatgttggg ccggacgtta
    16081 atgacttcgg gcacgacgct gctagcctta attgcactat atatttttgg cggcgaggtt
    16141 attcagggct ttatttatgc actgattttc gggattgtcg tcgggactta ttcatctgtc
    16201 tttgtggcgg cgccggtttt gctcaatacc ggggtgaaac gcggtgaagt ccagagcggg
    16261 gatactacgc cgcagaaggc ataatcctca tggacgtgac gccgcttatt aggtccgggc
    16321 aacaaatcat tcaaagttat agtggtgggc ggtttcgcgt gagcggggct gtgtatgagg
    16381 gcgctttgat tgtgatgcct gaagggtcac agcgctggga ctttgcgggc gaagtggcgg
    16441 acttgagcga gcctgatttt gttccgctta tagagcaggc tgcggagatt gatgtggttt
    16501 tgctgggctg tggggcgaca attcagtttt tatcttcaaa tttgaaaaat gcgttgaaag
    16561 cgtgcggtct taacgttgag gttatggaca gcggcgcggc gtgccggact tacaatgttt
    16621 tgatggctga tggacgtaga gttgccgcgg ctttattacc attttaagtt aaatctttct
    16681 taacatcctt tctatatcat aaacgcataa aaaaccgctg taattggcgg atagcgaggt
    16741 tttacgcgtg aatttgaagg gtgtgaatga tggcgcagag cgtttcagaa caagttcgtg
    16801 gattgataga aagtgaaggt gcgagcgcgc cggaatttgg tgtgcgtgag gctgaagggc
    16861 aggttgccgg ttggccggac tctttgccta tgccggttat tcttgaggtc ggcgggcgta
    16921 gcggggttgg ctatattact gatgaaaacg ggcagacgat gttgttgatg catgacccgc
    16981 aggcgccggg gcaggtttta gcctatgatg cagagcagat gcgccgtgat tatgatgcgg
    17041 ttgcaacgcc gttgattgag gatttaaagg cgacaagtcc gcgcgatatt tattatgggc
    17101 aagatttggg ggccgggtct tccgagagtt tggtgcggat aaccagcggt gactttgatg
    17161 gtgaaattcg ccggaaagtc gttgtgggtg aaggtggaaa tatcgaacgt cttggccctc
    17221 ttaagcttgg tgagcacagg cttgggaaaa gtgcttattt ggttgatccc actttacggc
    17281 ttggcgatcg gcttccagag gatgggcgcg tttttgctgc tgttgtggtt catgaggatg
    17341 ggagtattaa acatcatgcc cctgagcatg aggcatatcg tcatagtttt gcagatcgcc
    17401 cggaagttgg ggcgttatat gctcagttaa gtgcgtataa ttctacgatt atctatcaga
    17461 acagagggcc aggggcagaa ggcggtattg cggttaagca aaaggtaagt gcggaggtgc
    17521 tggagaatct gtctattcgt gtgcatgagc gtgatgctgc ggtaccgtct tatgaagcgg
    17581 tagggtcagt ggtgcaggtt gcgccagagc cgggcgtgcc ggaatctcag ccagttgtac
    17641 cagaagttgt tgtgtcagag tattatccgt cggcttttgt tcagaccgct gaacagcaga
    17701 gtgtagacgg cggatctgcc ttcattgcgc aaaaaatcgg aaacgccgag tctcatatgg
    17761 gaaatatggc ggctcttgat cgtgaggcgt tttctcaggc acgtgttatt ttaccgggtt
    17821 tggagaggga gcttcaaaaa agtcctgatt tgagcgatcc ggctgtgcag cagcaaatag
    17881 agttcatgtt taataatgat ttcgggattg cggatagcta taaggcggtg gcagcgcagt
    17941 ttgttgaaga gcgtttggcg gcgggtgggc cgattgatat gacgccatat aataacgcgc
    18001 tggaggcttt gcgtgatgga gacgtaagcc gggcgcaagg gcttttgcac ggggaagtgt
    18061 ctgagtcttc tgttgatgta gaacaggggc ggggggcaaa ggttgaggag tttcggggca
    18121 atgcacattc tattgaaccg gtcgctggag aggttgctcc gcactttaaa aatcaggaac
    18181 cttcttctgt ggaggggaga gaaaatgtgg cgttgaactc tgcgctggaa agtgcggtct
    18241 cggatttaca gagcgctatt gctacaggag aaaatgtcga tcctgcgagt tatgagggga
    18301 tggggcttag ttctgaagcg aagacagcat gcatgcagta tcaggagttg acggggtata
    18361 aatattcttt ggatcattct cagcaggtgc ttgcaggggt tggcgtgaat atttctgatg
    18421 caattcagct tggtgcagat ggcagaattc aaattcatcc tgataaattg ccgcaggaag
    18481 cggcgggtct taaggacgag tttgcggcca ttaaagagcg ggtggatgca atgcctccag
    18541 caaatgcgct ggaagcgcgc tgtgattaga gttaggcgtt tatgcggcta atttgaacgg
    18601 ggcttccgtt tttgcccgat ggtttcagcg cgacgcgttt tcgaacagct cggcgacata
    18661 ttcccaattg acgagattat cgacgaaggc ttcaaggtat ttgggccggg cattacggta
    18721 atcgatgtaa tagctgtgtt cccagacatc gcagcccaat agcgcggttt ggcccgatgt
    18781 gagcgggttg tcggcgttgg ctgttttggt gacttccagc ttgccttcag cgttcatgac
    18841 cagccagcac cagccggagc cgaactgggt tgcgccggca ttgaggaatt gttcacggaa
    18901 ggcctcaaat gagccgaagc tgtcagtgat ggcggcttcc agattgctgg gacgggcgcc
    18961 gccgccattt ggtttcatcc agttccagaa atgggtgtgg ttccagtgct gtccggcatt
    19021 gttgaacagg ccttgctcgc cttgtttgtg ggcgttgatg acaacttctt cgagcgtgtt
    19081 gcccatgtgg tctaaccctt cgaggatttt attgccgttg acgatgtagg cgttgtggtg
    19141 tttgtcatga tggaattcaa gcgtttcggc ggacatgtac gggtccagtg catcgtaggc
    19201 gtaggggagt tcgggcaaat cgaaggcgga catggtttag ctccttagct tttctttgat
    19261 ggtagaaact gttaagccag taaattaatt gttttacagg aataatcaat ggttaagacg
    19321 atttcatatt gtgggcggtt ggttcgggag catgacccgg accggttttt actttcaatg
    19381 tttgtgcctg cctcggtgcg tgaggggttg tgggcgttgt ttgcgcttaa ttatgagatt
    19441 gccaagacgc gggaagtggt cagcgagacg caattggggc tgatccggtt gcagtggtgg
    19501 cgtgagcgga tcgccgaaat ctatgacggg aaaatgccgc cgggtaatga gattttggag
    19561 tctctggcgg aggcgattgc gcgttatgat ttgccgcaag aacattttga tacactcttg
    19621 catgtgcggg agtttgattt ggaagatgtg cttcccggta atgttgaggg gctggtgaat
    19681 tatgcggatt ttacgtctac gccattgttg aagttggcga tgcaggtggt tggtggggat
    19741 gtccagcgtg agcccgtgca ggttgtggcg gtgaatgccg gtttggtcgg gattttacgc
    19801 gcggtttcct ttcatgcgcg ccaacggcgg tgttatttac cggaggattt gttgaaagag
    19861 gcggggcagc ggctggatca tttgtatgaa tttaagccgg ttgaggggtt gtcagatgtt
    19921 ataaaatctg ttttagaaca gtttgttgat ggtgtttctt gtgagaatat tttattgcga
    19981 aaacagcaga agctggcgga aatttacatg tggcagctga aatcctgtga ttatgatgtg
    20041 tttcattcaa ggatggcggt ggctccggct tttaaggttt tacgtttaat attttgatta
    20101 tgtaattgaa tcttaacgat tttgttgtta ggatagtgat ggtcaggatt tttctgagtt
    20161 agggtagaga ttatggttga ttccattcaa ggggcgggcg gtccgcagaa tttatcagcg
    20221 gctaatcgcg gtcaaaataa caaagtttct gaagataagc gcgttgaaaa aacagcgtct
    20281 caagcgcctg tgagagatga ggtgcaaatt tcagaagagg cgctgtcgtt atcacgagac
    20341 gagcaggcgg ccgagcagac tgctaaagaa acgcgcacga ttttgcaaga gcagcttgat
    20401 gagacgcttt cggcggatcg gcagcgggtt gataaatttc tttaagcatt cttaagcgtg
    20461 tttaaatttc ccattttgca ggaaatgtaa ggtttgctcc atgactttgg ggttgaacat
    20521 catgaagctg tgcgtggcgg gtagtactat gtggtcgctc atgccgtcga ttttggtgcg
    20581 ctctacggga acgatgccgt cgtgctgtcc atctaaaatc cagggagcaa gagggttgac
    20641 agaggccgat ccggcgatga tgccgagagg gtaggtgata tcatcgtcaa tatgcttgta
    20701 gcctgttttg agctggcctg aggcggggcc gaaaatcgaa cgaaatatgg gccccagcca
    20761 ttcattatca tcgaggaagt cggcaaattc gctgcccgtg ttgggtgggc cgagcatgac
    20821 gactttgtcc agattttcag gtttatgggt agcgatgaag tagcgcgtaa tgaggccgcc
    20881 cattgagtgg gttacaaaat tgagagggcg tttggggctg tatccggggt ggttctctat
    20941 tttttcggcg acaaagtcgg tgaggtcttc gaggcttttt tcgcgtgagg ggtagaggat
    21001 gttgatggtc tcgtagccgt ttttctcaag aaaattttcg agcagcagca tgtcagtttt
    21061 ggagcgcaga attccgtgca gtaaaacaac gatatcttta ggcggctttt cggtcattgt
    21121 tcatccagtt ttcaaggtct tcaagtgcgc gggtagtata cgctttttta cgatctttac
    21181 ctttgagttt tttacgtttg ccgtttggca tggtgataaa tggattttcg ggaaccggaa
    21241 agaggccgaa attgatgttc atgggctgga aggtttcgct tttcgcgccg cctgttatgt
    21301 ggccaagcaa tgcaccgtga gcggtggtga tgggcggggc ttcgaacggg atgcccagta
    21361 gctcactggc ggccatgcgt ccgctcatca ggccaacgga ggcgctttca acgtagcctt
    21421 cgcagcccgt gatttgtccg gcgaagcgga tttgtgggcg gaatttcagg cggagctggg
    21481 catcgagcaa tttgggcgag ttgatgaagg tgttgcggtg gaggccgccg aggcgggcga
    21541 actgcgcatt ttcaaggccg gggattgttt tgaaaatgcg ggtttgttcg ccccatttca
    21601 ttttcgtctg gaagccgacg atgttgtaga gcgttcccag cgcattgtcc tggcggagct
    21661 ggacgatggc gtagggttgt tcgttttcac ccttatgcgg gttggtgagg ccgacgggtt
    21721 tcatcgggcc aaagcgcaag gtttcggggc cgcgggccgc cataatctca atgggcatgc
    21781 agccgtcgaa atagggcgtg tcgatttcgg cttcggccag tgtttgtgct ccggagggga
    21841 gttcccattc cttgaacagg ccatactcgg cgtttatgag ttcgcagacg aaagtttcgt
    21901 attggtcttt ggtcatcggg cagttgatgt agtccttgcc cgtgccgatt ccttcagctt
    21961 tgtcgtagcg cgattgcatc caggcgatgt ccatgttgat ggactcaaaa tggacgatgg
    22021 gggccagagc atcaaagaag gacagctcat cctcgccggt ttgttctttg atccatgcgg
    22081 cgagcggatc ggaggtgagc gggccggtgg cgatgatgac attattccat tcgggcggaa
    22141 tttctgtgat ttcgccggtt tggattgtga tattcgggtg gtcttggagg gctttggtaa
    22201 cacctgcgga gaaaatatcg cggtcaacgg ccagcgcgcc gccagcgggg acggcggctt
    22261 tttcgccttc gcgcatgatg agggaattga gcatacgcat ttcctcatgc agcaggccta
    22321 cggcgttata ttcggcgtcg tcagagcgga aggagtttga gcaaacgagc tcggcgcaat
    22381 tgcctgtatt gtgggcgggg gtgcctttgt cagggcgcat ttcgtgcaag ataacatgtg
    22441 cgccgagatt agcggcttgc caggcggctt ctgaaccggc gaggccggcg ccgatgatgt
    22501 gaatggtttt gctcattggc tggttttaaa cttcaaccct gtgacgggtc aaggttgttt
    22561 ctggtatgcc atgccccacc aaattgtctt cctctggtaa tattggaaag ttttggcgga
    22621 tctaatcttt cttgcagggc tgcaaatcgt ttgccttgtt tgacggtgtt aattctatca
    22681 aacaaaatat ctcgaataca ttctgcttgt agccatgctc cggaaagccc gagacattct
    22741 tgtagtgtga gaagatcgtc gaatgttcta tcttgttctt ttataccgtt atcaataagc
    22801 gaaatagcat tttcatagtt gccgcgcatg gttgcctgtt ttgcttgtcg gcgtattttt
    22861 gtgttctttt tattattcat agtttggatt tttaacatat cgcctaatta tatgtaaact
    22921 atatttttgg gaaacagtct taaacaatcc ttttcttttg gcggtgaggt tgtaaactcg
    22981 attcattcgt tacaccaacc ggattgaagt ggggatcacc gatgaaaaaa tttcttttaa
    23041 gcactgtttt ttcttttgtt tttgttatgc cagggtgggc tgaggaagcg gtggtgcctg
    23101 tcgatgcggt gcagggaaaa gtgcagcctg ttattgaaga aactgcggat ggtgttgtga
    23161 aggtggtgca ggaagcggtg gtgcctgcgt atgcgcaaga ggctggcact gatgttcagg
    23221 ttgtaaaagc cgagtcatcc gctggagatc gtatgtctgc agatgaaggg catggtgata
    23281 tggatgctga gacggtggcg gtagaacatc atggcactgc acaggcgcat tggggatata
    23341 gtggcggtga ggcggcgccg tattggggta ctctggatgg ttcttatgag ctttgtgcaa
    23401 gcggtcaaga gcaatcaccg gttaatattg ccaagttttt acaggaagac gtgccggata
    23461 ttacgctttc ctataagccg tcgtctttga atgtggtgaa taacgggcat acgattcagg
    23521 tgaattatga tgcaggaagc ggttttgagg cggctggtgt gaactataat ttattgcagc
    23581 ttcattttca tacgcccagt gagcattatt tagacggagc gccgtatccc atggaaatgc
    23641 atttggtgca taaggctgcg gatggaacgc tgggtgtgat tggcgtgatg atgaaaatag
    23701 gggggcataa ccctgtgatt gaaggggttt ggcaaaatgc gccgattcag gcaggtggaa
    23761 gcaaaagcat tgaaagtgtt gaaatcaatg cggcagattt gatgcctgca tctttggagt
    23821 attacaaata tgatgggtcg ttgacgactc ctccatgcag cgaaggtgtg cgctggcatg
    23881 tgttgaaaga gcccattgag atttccgaga tgcagcttaa ggcttttcag gcgcttttcc
    23941 cggtgaatgc acggcctgtg cagtcgctta atgacagagt tattactgga gattaatgga
    24001 aagtcttctt gcagaaattc agtatcacgc ttcgcaggct ccagatcgtg agatgtttgc
    24061 gctttggcgg gcagtgtttt cactggcgca cggggatggg gagttggctg aggtcgagca
    24121 gaatctggtt aaacaggtga tgggtgtttt tagtttttcg cctgagcagt gtgccgttat
    24181 tgagaaagat atgcatgtgc cttgtgatgc gcgccatttg tttcaagaaa ttgaactttc
    24241 cgaatatcga agccagtttt ttcgtttggc gcggattgtg gtttggtgtg acggtgtttt
    24301 acatgatgat gagcttgcgg ttattgaaag tattcaggat gatttgggtg atgaggcggc
    24361 ggtttatgag gcggatttac gctggatgaa tcgcaagcct gatttgccgt tgggagagca
    24421 ggcggccagt ccggaagaag agatgcttaa acaggtgatt tatcagatga ttgcatttta
    24481 tcagcaggaa ggtgtgggat gagtgaagct gtaaccgata gtcagtttta tatgtggcgg
    24541 actctgtttg ctgtggcaca tgcggatgat gttgtttcaa atgaagagct gcgttttatg
    24601 gcggaggcgt tggatgatat tccgttttcg gatgagcagc gggcgatttt aaacgaagat
    24661 gtaaaaacgg cgcaggatat tgagaaaatg tttgccggga ttacggacgt gcgtgatcag
    24721 gcgcattttt tccaattcgc acgggagctt gtgcatattg atggggatta tagcaaagaa
    24781 gagcaagcgg tgatgttgaa acttgaagag gcgcatttga aatgtacgaa tctggacgag
    24841 cttattggta gtgttggtct tgagtttgaa gatgattcgc aagggatggt gtttgcaagt
    24901 gcgaagcgga agaaaagctt taaagatatt gtgtattcct atcgtgatga atttttgaaa
    24961 aatcgctttc ataagtaagg tgggtttctt tttataggca cgtaaaaacc aaggggcttg
    25021 ccccgtggat ggagtgcgtc cag
//
LOCUS       DAOSWR010000010        24545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1308_length_24545_cov_5.685259, whole genome shotgun sequence.
ACCESSION   DAOSWR010000010 DAOSWR010000000
VERSION     DAOSWR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 24545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24545
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_24545_cov_5.685259"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK513_01620"
     CDS             <1..407
                     /locus_tag="PK513_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HOO81182.1"
                     /translation="NGGDGSDSLYGGTGMDTLVCDAGIDFLYGGVDASVDVFGFSAAE
                     DSQDRIYNFTLGIDQINITDLLSGYSHGSSDIDDFVLIVHTGSRFDIQVDRYGGGDSY
                     VQTARVFTDIADSLTAQDLLDSGALIANSTLA"
     gene            complement(410..1102)
                     /locus_tag="PK513_01625"
     CDS             complement(410..1102)
                     /locus_tag="PK513_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO81183.1"
                     /translation="MIITASMHNVSKTITKDDERYIHKIISAQNYDWQDIKHNTTFEE
                     ELEDIRAIQSAVLNTTPLQKQVPDNRPREPKDLFELGYAQCSDRSRVIDKMLRLAGFE
                     TRVASLYSTAKTGSAIKSLLSNDKDLVHSHSIVEVKTRRGWMMVDTNEQWLGLDKEMQ
                     PVSLERWYTQVKNGNSDIWHTSNEGKIYWLMEGPYTFVYGLYSRHGYFYPPYNCVPDI
                     NWPEFRYNFFRK"
     gene            1158..2363
                     /locus_tag="PK513_01630"
     CDS             1158..2363
                     /locus_tag="PK513_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81184.1"
                     /translation="MVESSESVSFFFKIIWVAYLVLAPFYVLQSGYPQPADFVLLLGL
                     APALGLAALKSEVRIPTAILLGIGFVVLTFTINIIHFVFTSDLKFLLSSLYYVYNFGV
                     FLFALQLFKLAPQTINHLTYWALAASICIETVFILLDYGSSIRTQGTFNNPNQLGYWS
                     LLSFALLIIIKRHERLTLMDAALILMMFVTQLVSLSKAGIVSMAFCLLIVFFSRLMSD
                     TSRLFLIGIVSVGLAFMLITQNDLLSKVADFKLIDTVYTRISTIGAQGDDSLRGRGYD
                     RILDNPHYVLLGAGEGANWRYNPQGKELHSGLATLLFSYGIVGFLLFFTFLFFVFRGR
                     PWRYIFLLVPIMMYGLTHQNIRFTYFWVVIAAAYSGCHFEQRAGMYKPTLLTDDSDVG
                     KAPPRQHAS"
     gene            complement(2307..2999)
                     /locus_tag="PK513_01635"
     CDS             complement(2307..2999)
                     /locus_tag="PK513_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO81185.1"
                     /translation="MSSKISSWEEQGNKHINIKGKKHPLPYHKIISDLAIQYSQDGDA
                     VLDIGCGLGQTVALIQDKGQREIHIADAYENCLEITCNRISPQAEYLIDERNFDISNK
                     IQRTFKVVIMSHVLEHMLNPVQAVHDALGLVEDGGYLILAVPNPARPGVLISNITKTH
                     YVNRGHVMTWDMSHWINFLENILKLDVVEYRNDFIQIKGCNSLPAIMSLGETLGRVLP
                     WWCFSNIAVIRK"
     gene            complement(2996..3877)
                     /locus_tag="PK513_01640"
     CDS             complement(2996..3877)
                     /locus_tag="PK513_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOO81186.1"
                     /translation="MSKPDFIGVGTERAGTSWLFSMIAHHPESWVPPLKELHFLDTID
                     PDVPSHNPRYRWHLTSRIKQKATPFMNISHRPEFLKNTAFEYLLWDACYFSGSMNFEW
                     YQRLFAERFVKGRIAGEYTPAYCNISEALIKKMLELNPYMKFLLVFRHPCQQIRSSLI
                     QHFVMIENRPFASVGEDEMMEWLCAPFAQKKSNIGDVFEKWSRCLPQDQLFIGLYEEI
                     KQAPEELIKRVYRFLGLNESFMPDRKFYANKINNLTKPDYIIPQIVLDYIDEHWGREA
                     EHIQKNMPQLAQYWSNL"
     gene            complement(3870..5216)
                     /locus_tag="PK513_01645"
     CDS             complement(3870..5216)
                     /locus_tag="PK513_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOO81187.1"
                     /translation="MSIEQPPQGFVKLLVRLFSVQAIGLVCGFLSHLLLARMLAPEQY
                     GVFNFIFSSAMLCALLGNFGFQASSVRLIPQFTLAKQASLLRKFYGFSFAWVLGMSTL
                     AGLGLYGLYANVLLSEDYTQTTLIIGVILVLFIALIKLNSGVLKGFKKESLALTYESG
                     LKEILLLGFLAIVFLAGFSMSSAVVVLIALVCIFLGLLITSSTHAIALVRPLKPVSEE
                     GKKQQAAENIKDENQIQLYRRWLRISLPMMLVISAQMLIHRSDIVLLGLLSSASDVGL
                     YSAGAKLAQAATIAMMALNIVFSPRAAEAFHQNDTSGLCNLYGKIMKLQAISTATMAI
                     CIALGAQVVLNFIGTEYAQALPVVYVLLTGYVLNAFWGPVPFLMIMTKYEDQAMWITF
                     AAALLNIMLNIVLIPVYGIVGAAISTVIALNLRNAGALLYVLSKGLLSESRKQTDE"
     gene            complement(5207..7111)
                     /gene="asnB"
                     /locus_tag="PK513_01650"
     CDS             complement(5207..7111)
                     /gene="asnB"
                     /locus_tag="PK513_01650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO81188.1"
                     /translation="MCGIAGYFQIQKTENLEHVVQAMADALTHRGPDDHGMWIEPQEK
                     RLAFAHRRLSVIDLSKAGAQPMRSSSGRYVISYNGEIYNFQALRNELEKFGCGFQGHS
                     DTEVLLAGFAHWGIKDTLKRVAGMFALALFDCKERTLTLARDRCGKKPLYFGWDKGVN
                     VFAFASELKAFTAVQAFRKPDINNKAIQLYLQWRYIPDPHSIYENIWKLPPAQFITRP
                     LEDFIHPFTPQEHAETYWHFESVTGQERQSAAEHSQLNGLEDILTQAIKERMIADVSL
                     GVFLSGGVDSALITALMQKHADQPVKSFTIAFDDSRYNEAPHAAQISAHLGTEHTEML
                     LTSAEAMKTARHINSLFDEPFGDPSAIPTYHVCRLAKKELTVALTGDGGDETFGGYSW
                     YFRAQKLLPVLKLPNGLRRTVGQALTALPLAGGLFSAAQRRKISALIAADDPDELYPL
                     LHSYWSGVTGQRAQNIDDTAYGSMIETLPVSNPIERMMAFDTRMFMAGDVLTKTDRCS
                     MAHGLELRSPLLDHRVIEYGWSMSHDMKIRGHHGKYALKQLLRRYIPDALIDRPKQGF
                     SIPHGRWLREDLRDWAEDMLCVSTLKKHSLIDPDFVSLYWDAHASGQADYGHYLWTIV
                     CLQNWAETCR"
     gene            7373..8605
                     /locus_tag="PK513_01655"
     CDS             7373..8605
                     /locus_tag="PK513_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOO81189.1"
                     /translation="MTVFAQESLESEPFAFHAEPSPDLSSALADPFTYQGEPIVFNGF
                     RLWPNLTLEQRYTDNLLATENNTQSDFITVLKPELEIWKSLGRHDFSLLMNSEILQHW
                     DRSQDNVENYNVAFNADLEARQGLNIPIKLLYRDGHLRRAYQRRSNADQIVKTPLNVK
                     RWRAESGFIYKPNRLMLSLLGDYSQVRLGNEELINGGFLIRDNRDVNITQATGKVSYD
                     LKTGLSPFVQTSYTKENYINETPGAVSRNNDSLRILAGADFNFKGLIAGFIGAGIDRR
                     SYDDATVGNTGDVLLDAEIYWDPIPKTRLRFDARRETTEDNLLIQQLTETAFGLGIDH
                     ELRQNLFARLAAHYGYDEFMDTNRQDKTLDSHFELLKIIGPHLQLGAGYQYLTKDSSV
                     PGLDMDQSVVFLRIKTAL"
     gene            complement(8600..9352)
                     /locus_tag="PK513_01660"
     CDS             complement(8600..9352)
                     /locus_tag="PK513_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81190.1"
                     /translation="MIKQPKIIFHHVPKCGGTAIITGLAITYYPLRLLRYGREGFSGA
                     LNARAASYSAEMHNECQYAFRRKLLSYYAEKGDSPLICGHYPFNHDFHAAHKDEWTFV
                     TLLRDPLQRWYSEYFWNRHKDHEYRKTDLSIEEYLESRNGMMNARSFVNFFSRSEDMY
                     ETPRSQDVKQAIETLEQIDVVGCLENIDNFKARMQAKLGRTPIFFKRNKSPASQEQKK
                     VPDKNSDFHKALLERLQGDIEIYEKTKERLGL"
     gene            9434..10630
                     /locus_tag="PK513_01665"
     CDS             9434..10630
                     /locus_tag="PK513_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO81191.1"
                     /translation="MRVLYCHDNIYQQCESGTVYSPGQFPYEYWKIFLDNFDHLVVIG
                     RGAGLHDKIDQLNISSGPRVSFALFPNINTPVGRLKYARRVNKRLERIVADADAVIIR
                     AVSDMGWLAFKHALRMNKPIALEMAACAWDSTWNHGNKLGKIYAPIRYLHDRIIARHA
                     DFVMYVSYVFLQRRYPTNGEVAHISNVRIDKPDEQYVDQRLKAIKVKQDEMTAPYTIG
                     LIGNLDNRIKGVDLTLRALAKVEEEKPGCFIFHHLGPGDPEPHMALAHALGISERIRF
                     DGMIKSGEGVMKWLRKVDLYLQPSFQEGIPRATIEAMSTACPIIASTAGGIPELIKEE
                     WLIKPGDVDNLARLILKMLDNPKFQYEAGLNNYIASLDYTNDILVPRRQGFWGAFAKF
                     AREKAQ"
     gene            10761..11453
                     /locus_tag="PK513_01670"
     CDS             10761..11453
                     /locus_tag="PK513_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOO81192.1"
                     /translation="MYNTKLIVVSAVLCLAILAGCARGTLADIQTQNSSLSNERHEQN
                     QPMATEKVDAIANPIAIEPKKTFNDSCHEKEKNDAYRLGAEDRLDILVYGETELSGNY
                     TLDTTGHISMPLIGDVLLGGCNIRQAQEIIKNLYADGYLVDPSISVEVSNFRPFYILG
                     EVKSPGKYDYVAGMNVLKAAAIAGGFTYRANRKVAKVLRYDSYGEPVYHQQILEDEVQ
                     PGDVITIPEKLF"
     gene            11461..12888
                     /locus_tag="PK513_01675"
     CDS             11461..12888
                     /locus_tag="PK513_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HOO81193.1"
                     /translation="MAETTQNYYRSKEPGNYAGSDLDLSTLFRVMWRRKISILFFLIL
                     GVTVTFVSLSYMKPLYTSQAVILIQNNAGKLAIKDLSQLIKSKSLNTDFVLTEIEIIK
                     SRNMVSQVVKRLGLIDDPELSNHTHVPQNDQNFKALLVDGTTFESLPPETLDAKVSMT
                     INKFLERLRVVSIPGSTAVQINFTSENPYKSAIITNAIVDIYTNQKISEQTRLQQRLS
                     EWFDERLHNLREQALETDRKMQKYKIEHELTEDQTDIISTNHLNKLNEQLTAAQLKKS
                     EVAVTLQQLNKPDDFENIEALTSNDVNFGILKNLRLEELRLKTEISDLSNRYGPKHPV
                     IKKKKSEIDTIQKSLRSEVQEIKNALNTEIKIADAKAKDLERQIRSNIKNISNNADTL
                     MILRELEREADSAELALRTFLETYKNIAWQDMLPGSGVQVISDASVPTHPSFPNSPLF
                     LLLSAFISLFLGMSFAIFREHVAKN"
     gene            13086..16844
                     /locus_tag="PK513_01680"
     CDS             13086..16844
                     /locus_tag="PK513_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HOO81194.1"
                     /translation="MAGFFLTLTTSVFFSADAPNIEVLVNGVVSASFIETELEGSGST
                     TRTLYIDYVGLSPSDITVQINDASGETGRYLVLEAVAVNGQNIRNNTLNVNDGTPVDL
                     LTTLESMDFDTTNTDWLFGQTEPLLSYFGTSTYTGTGADDTQNGSHTVNDVMNMGGGN
                     DIANGRNGNDFMFGGAGNDLLVGADGHDVLVGGDGNDELRGQLGNDILYGGLGDDVLY
                     GNEGNDTLNGGAGNDLINGFHDDDVIYGEAGMDKLYGGDGNDTIFGGDDKDFIYGEAG
                     MDNLSGGEGDDIIEGGDDNDIIAGDGGNDRIWGDAGDDNINGGAGLDTIYGGLGIDTI
                     SGGDDNDRIEGNDGDDILNGDAGQDYIYGGLGNDIIDGGTEADILYGEEGDDIINGGD
                     QNDTLYGGDGIDTMDGGAGSDRIYGEADDDTIYGRGGDDIIEGGDGNDTIYGNGQNDI
                     LRGDAGDDYLKGGTGDDNLYGGDGNDDLRGDDGNDRLKGGAGNDQLRGGDGDDVLAGE
                     DGDDILYGQGGNDKLYGQAGNDDLRGGDGDDHLFGREGIDILQGNNGNDTLVYDGVDT
                     LDGGADIDTILVAGYDTVTIDFASGLISNMEAVNMANTNGIAAANNLQISMAQIAASS
                     DTGNMTITGDVGLDQVNFTLTGSETRGADVNIDGIDYAQFNDGGTTAYIQLGLIYNGS
                     VLTASGGGSSNTPTPGDDVLTGTSGEDTISALAGNDIIIGEAGNDTLSGDEGNDNIYG
                     GEGNDTLNGGDGNDILHASLSGSITPSTDPATVVANNPGVVYSSETGNFYQFVTASAN
                     YTTAAATAAAATLEGISGHLVTITTVAENTFIENLTGTNFAWMDGTDSVTEGTFVWTA
                     GPDAGTQFWNDIGNYTNFYQGSPTTNSNANDNVIFLGTTYSGTWYAYTGTYATNYLIE
                     WEGSDVIQEIIVGFDSRNETNYLNGGAGDDQLYGSMGRDILVGGTGADILEGGAGNDK
                     LYADGLSDAEIQAILTANPGVTYSEETGNFYQFVSGSFNYANALAGASSTTINGIAGH
                     LATITSAEENSFINGIVGANFVWIDGSDAATEGTFVWTTGPDAGTQFWNDIGNYENWY
                     QNSPTSNTNANDHVVFLGQNYNGQWYIFGDNTNLNGYVIEWETSSFGTDTSANSLNGG
                     YGADDLYASGGIDTFIFEAASAFSDIDTIHDFDNTADILDISDILSGINVDASNVADY
                     VSVDELTGVRVDVNGTGTFGAGTQIASFSSAVGVDDALTMFNNGDLIV"
     gene            complement(16917..16992)
                     /locus_tag="PK513_01685"
     tRNA            complement(16917..16992)
                     /locus_tag="PK513_01685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16957..16959),aa:Lys,seq:ttt)
     gene            complement(17056..17586)
                     /gene="coaD"
                     /locus_tag="PK513_01690"
     CDS             complement(17056..17586)
                     /gene="coaD"
                     /locus_tag="PK513_01690"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOO81195.1"
                     /translation="MTQNNGKYLIGVYPGTFDPVTKGHLHLIRRASRMVDKLIIGVAD
                     NARKGPLFTYEERVEMVRTDIENMQEKGCEIEVRSFNNLLVHFASEMQANCIFRGLRA
                     VSDFEYEFQLTGMNAKLDPDIETVFLMAADKWQFISASFVKEIASLGGDINEFVTPQV
                     AKAISRKFSKPSPKKA"
     gene            complement(17573..20362)
                     /gene="gyrA"
                     /locus_tag="PK513_01695"
     CDS             complement(17573..20362)
                     /gene="gyrA"
                     /locus_tag="PK513_01695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO81196.1"
                     /translation="MSDSSVIEDDQIPTISLEEEMKQSYLDYAMSVIVSRALPDVRDG
                     LKPVHRRILYAMREGGYTSDKPYKKSARVVGDVMGKYHPHGDAAIYDSMVRMAQPWSL
                     RLPLIDGQGNFGSMDGDSPAAMRYTEARMDKAAEVLLQDIEKETVDFRPNYDESLTEP
                     SVLPSRVPNLLVNGAGGIAVGMATNIPPHNLGEVIDACVAMVDNPNITTEEINEIIPG
                     PDFPTGAKIMGKNGIYKAYHTGNGSVVMRSKTSIEEIGKREAIIVHEIPYQVNKGKLL
                     ERLGEVGREKIVEDIAEIRDESDREGVRIVIELKVGVIADVVLNQLFKHTALQSNFAC
                     NMVALHNGKPQMMLIRDMIKAFVKFREEVITRRTIYELGKARDRAHILAGLAVAVANI
                     DEVIALIRGAPDPTTAREGLMAKSWPAKDVAPLIELIDDPEHPLNDDGTYQMSEAQAK
                     AILELRLHRLTGLERDKIAAELKEITDKIAEYLEILADRERLLEVLVEELKEIKDRFD
                     TPRRTELCEAEFEMDMEDLIQREDMVVTITHEGYVKRVPLDTYRAQRRGGKGRAGMST
                     KDEDFVSDLFVASTHTPILFFTSRGIVHKMKVYQLPLGSPTSKGKAIINLLPLEKDEK
                     VNVYMRMPEDVDVIDDLDIMFATSHGSVRRNKLTDFANIRSNGLIAMKLGEEESLVSV
                     KICTEDEDVMLATKMGKAIRFPVTDIRVFAGRNSTGVRGVKLAQKGDEVISMSILKHV
                     DITPDERNAYLKAASQIVGSDDEDFEYEETTLELSEERFRELLKKEQIVLTVSNKGFG
                     KRTSAYEYRTSGRGGQGVANMSLTKKNGGEVVATFPVTDSHQIMLVTDKGKLIRTPVE
                     NIRVTGRSAQGVTIFKVGDDENVVSVAWLVQEDDDEDEDELEGEVLPEEGDEKTESAQ
                     TQPPADEAAESDDAE"
     gene            complement(20372..21793)
                     /locus_tag="PK513_01700"
     CDS             complement(20372..21793)
                     /locus_tag="PK513_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOO81197.1"
                     /translation="MDAMLISFLVFFIFFTGVGLASSFFRKNNIEDYFLASRSIPAWL
                     VALSYGATISSGATFIGFAGLAYNSGLTAVYAVVGLTIGDHLGWIIAGNKIRRKAIET
                     KAHTYPSLVGKLAGRDFQIVTVITAMLTVVFLGTYCSAQLVAGAKIGEALFDWDYNIF
                     VFVGAFVLLAYCWAGGIRASIWTDAVQAIIIILSLMVLVWVAMEQIGGFGELYRQLKA
                     IDPALVNPAQTKFISVFIGWLAFGVGVLGQPQLMVRHMVARSDEDLLTARRIYLSWRW
                     SVLALATLVGFIARVMIPEIEGGFDPELSIPALWEDLLPPVFVGFMVAGLFSATMSTA
                     DSLLLSASSALTQHVFPQLRNSYALARLGTVAVICMIVAIALLASQNVLSLVVLAWGG
                     MASAVAPLVVLILFGEKPTQRLAVTMMVAGFGVAVYWRHGLGLQAQLMDLVPGMMTGF
                     AIFALSYLLEKYVRSEPPKNKSL"
     gene            complement(21824..22228)
                     /locus_tag="PK513_01705"
     CDS             complement(21824..22228)
                     /locus_tag="PK513_01705"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOO81198.1"
                     /translation="MFCAPAFAQGDDFPQRLKLAEKMLEIRPAKQQLETAINAYLERA
                     MAGASENDKEVVRSALLKIMNPKALEKITVDSYAEIFTLKELEAMVAYYNQPEARSAS
                     DKQEQLNARIAPEIVRMLDQALVRFRTETQSP"
     gene            complement(22265..22726)
                     /gene="ssb"
                     /locus_tag="PK513_01710"
     CDS             complement(22265..22726)
                     /gene="ssb"
                     /locus_tag="PK513_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOO81199.1"
                     /translation="MSGSLNKVTLIGNLGADPEVRSMQSGDEVCNLSIATSETWKDKA
                     SGERKEKTEWHRVVIFSPGLVNVCKNYLKKGSKIYLEGQLQTRSWEQDGQKKYTTEVV
                     LRGYDAKLVMLDGRSGNDNAGYSQGGGFSQGAPTQAQAAAPVDELEDEIPF"
     gene            22833..>24545
                     /gene="uvrA"
                     /locus_tag="PK513_01715"
     CDS             22833..>24545
                     /gene="uvrA"
                     /locus_tag="PK513_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO81200.1"
                     /translation="MSLKTISIRGAREHNLKDINVDMPRDKLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLELMQKPDVDSIEGLSPAISIEQKTTSRNPRSTVGT
                     VTEIYDYMRLLWARVGVPYSPATGKPITSQTVSEIVDRIMAMGDGTKLYLLAPIVRGR
                     KGEYRKEFKELQAKGFQRVKVDGTLYEIGDVPTLDKKLKHDIEVVVDRLVVREDMGNR
                     LADSVETALRLADGLVLVENAEDGTQELFSEKYACPVSGFTIPEIEPRLFSFNSPHGA
                     CPTCDGLGKRMYMDEALVVPDGSKSLLKGAIEPWSKSFAPFYMQALEGVAAHYGFSAA
                     EKWDKLPRKYQEIILYGSGDEPIAITYNTKTESTWKSNKPFEGVIPSLERRMIETDSN
                     SAREELAKYQASAPCEACEGHRLKPEALAVKIDGKNISEVGTYSIEEAGEWFERLSHP
                     VIASEAKKSRKQKNGLPRPADADLAMTNEEILTPQQYQIAEKILKEINERLNFLRNVG
                     LDYLNLSRNSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNNRLLETLT
                     RLRDM"
BASE COUNT         6549 a         5572 c         6030 g         6394 t
ORIGIN      
        1 taaacggcgg tgacggcagc gatagtctct atggcggaac cggtatggat acgctggttt
       61 gcgatgccgg cattgacttc ctctatggcg gtgttgatgc gagtgtcgat gtgtttggct
      121 ttagcgctgc agaagacagc caggaccgta tttataattt cacgcttggc atcgatcaga
      181 ttaacattac ggatttgttg agcgggtatt cacacgggtc atctgatatt gatgattttg
      241 tgctgattgt tcatacaggc tcacgctttg atatacaagt agaccgctac ggcggtggcg
      301 atagttacgt gcagacggca cgcgttttca ctgatattgc cgatagcctt acagcgcagg
      361 acttactgga cagcggcgca ttgattgcca atagcactct ggcataaagc tattttctaa
      421 aaaaattata tctgaattca ggccagttaa tatcgggtac gcagttatag ggcggataga
      481 aatagccgtg gcgtgaatag aggccataaa cgaacgtata aggaccttcc atcaaccagt
      541 aaatttttcc ttcgttggat gtatgccaga tatcagaatt tccatttttt acttgcgtat
      601 accaacgttc aaggctgaca ggttgcattt ctttatcaag acccagccac tgttcgttgg
      661 tatccaccat catccagcca cgccgggttt taacttcgac aatagaatgc gagtgcacaa
      721 ggtccttatc atttgaaagc agtgatttga tcgctgaacc agtctttgcc gtgctgtata
      781 aactggccac ccgggtttca aatccggcca gacgaagcat tttgtcaatg acccgactac
      841 gatcactgca ttgagcatag ccaagctcaa acagatcttt gggttcacgc gggcgattat
      901 cgggcacctg tttctggaga ggagtcgtat tcaaaacggc ggattgaata gcccgaatat
      961 cttccagttc ttcttcaaac gttgtgttgt gtttgatgtc ctgccaatca tagttttgcg
     1021 ctgaaatgat tttgtgaatg tagcgttcat catcttttgt tatagttttg gaaacattgt
     1081 gcatagaggc agttataatc aatgcgcaaa tccctgtaac acctatcgat aataaaaata
     1141 tcctggtcgg ttttttcatg gttgagtctt cggaatcagt ttcattcttc tttaagatta
     1201 tttgggttgc ctatcttgtg ctggccccat tttatgtact gcaaagtgga tatccacagc
     1261 ctgctgattt tgttctgctt ttaggactag cgcccgcatt gggactagca gctttaaaaa
     1321 gcgaggttcg cattccaaca gcaattcttc tgggtatagg gttcgtagtg ctaacattta
     1381 ctatcaatat catacatttt gtttttacgt ctgacctcaa atttttactg agttcgcttt
     1441 actatgttta taacttcgga gtgtttttgt ttgctctaca gctttttaaa cttgcgccgc
     1501 aaacgatcaa ccacctgaca tattgggcac tggcagccag tatttgcatc gaaacggtct
     1561 ttattctgtt agactatgga agttcgatac gtacgcaagg aactttcaat aaccccaacc
     1621 agcttggcta ttggagcctg ctgtcctttg cgctgctaat cattataaaa cgccatgaac
     1681 ggctcacatt aatggatgct gccctgatcc tgatgatgtt tgtcacgcag cttgtatcat
     1741 tgtctaaggc aggaatcgtt tccatggcgt tttgtttgtt gatcgtattt ttttcccggc
     1801 tgatgtctga tacgtcacga ctttttttga ttgggatcgt gtcggttggt ttagctttta
     1861 tgttgattac gcagaacgat ttattgtcga aagttgcaga tttcaagctg atcgatacgg
     1921 tatatacgcg tatcagtacg atcggtgctc aaggcgatga tagtcttcgt ggacgcgggt
     1981 atgaccggat actagacaat ccacattatg tgcttttagg ggcgggcgaa ggtgcaaatt
     2041 ggcgttataa cccgcaagga aaagagcttc attccgggct agcaacgctt ttgttttcct
     2101 atggtatagt tggatttttg ttgttcttta cgtttttatt ttttgttttc aggggacgcc
     2161 cctggcgata catcttcttg ctggtgccta tcatgatgta cgggctaaca catcaaaata
     2221 tccgctttac ctatttttgg gtggttatcg ccgccgctta ttcaggatgc catttcgaac
     2281 agcgcgctgg aatgtataaa ccgacgttac ttacggatga cagcgatgtt ggaaaagcac
     2341 caccacggca acacgcgtcc taacgtttct cccaatgaca tgatagccgg gagactgtta
     2401 caacctttga tctgaataaa gtcattgcgg tattcaacaa cgtcaagctt cagaatattt
     2461 tcaagaaaat taatccagtg tgacatgtcc catgtcatga cgtgaccacg attgacgtaa
     2521 tgagttttcg taatattaga gattaaaacg ccgggtcggg caggattggg cacggcaaga
     2581 ataagataac caccatcttc aaccagccct agagcatcat ggacagcctg aacggggttg
     2641 agcatatgct ctagcacatg cgacatgatt acgaccttaa atgtgcgctg gattttgttg
     2701 gagatatcaa aattgcgctc atcaattaaa tattctgctt gcggggatat gcggttgcag
     2761 gtgatctcca gacagttttc gtaagcatca gcaatatgaa tttcccgctg gcctttatct
     2821 tgaatgagcg ccacagtctg ccccagcccg cacccaatat ccaaaaccgc atccccatcc
     2881 tgggaatact ggatggccag atcactgata attttatgat agggcagcgg gtgtttttta
     2941 cccttgatgt tgatgtgttt attgccctgt tcttcccagg acgaaatctt gctactcata
     3001 aattgctcca gtattgggcc agctgcggca tgtttttttg aatatgctcg gcttcgcggc
     3061 cccaatgttc atcgatataa tcaagcacga tttgcggaat aatataatcc ggcttggtca
     3121 ggttgttgat tttgtttgcg taaaacttcc tatcaggcat gaagctttca ttgagcccta
     3181 aaaagcgata aacacgtttt attagctcct ccggcgcctg tttgatctct tcatacaaac
     3241 caataaaaag ttggtcttgc ggcaggcatc ggctccattt ttcaaataca tccccaatat
     3301 tcgatttttt ctgcgcaaaa ggagcgcaca gccattccat catttcgtct tcacccacag
     3361 atgcaaaagg tctattctca atcatcacaa aatgttgaat taggctggat cggatttgct
     3421 gacagggatg acgaaagaca agcaggaatt tcatgtaagg gttcaactcc aacatttttt
     3481 taatcaatgc ttcggagata ttgcaatagg ccggcgtgta ttcaccggca attcgccctt
     3541 taacaaaacg ctcggcgaat aagcgctgat accattcaaa attcattgaa cctgaaaaat
     3601 aacaggcatc ccacagcaaa tattcgaaag ccgtattctt taaaaactcg ggccggtggg
     3661 atatgttcat aaagggtgtg gccttttgtt taatccggct ggtgagatgc cagcgatagc
     3721 gagggttatg tgatggaaca tcaggatcga ttgtatcaag aaaatgcagt tccttgagcg
     3781 gaggcaccca agattccgga tgatgagcga tcatcgaaaa aagccaggat gttcctgcac
     3841 gctccgtacc aacaccgata aaatccggtt tactcatctg tttgttttct gctttccgat
     3901 aacaggcctt ttgacaagac atataacaac gcaccagcat tgcgcagatt aagagcaatg
     3961 accgtcgata tcgctgcccc tacaatacca tagaccggga tgagtacaat attgagcata
     4021 atgttcagca gagcagcggc gaacgtaatc cacatcgcct gatcttcgta ttttgtcatg
     4081 atcatcagga acggtacagg tccccagaat gcattcagga catatccagt cagcaggacg
     4141 tagacaacag gtaaagcttg tgcatattcc gtcccaataa aatttagaac gacctgcgca
     4201 cctagcgcga tacaaatggc catcgtcgca gtcgatatag cctgcagttt catgatctta
     4261 ccatacaggt tgcatagacc ggacgtatca ttctggtgaa acgcttcggc ggcgcgcggg
     4321 gaaaaaacaa tattaagcgc catcattgcg atggttgcgg cctgcgcgag tttagcgccc
     4381 gcactataca aaccaacatc gctcgcgctg gataaaagcc cgagcaagac aatatcagag
     4441 cggtgaataa gcatttgcgc ggaaatgacc agcatcatcg gcagggatat ccttaaccaa
     4501 cgccggtaaa gctgtatctg gttttcgtct ttgatgtttt cggctgcttg ttgttttttc
     4561 ccctcttcgg atactggttt taaaggccgc accaaagcga ttgcatgcgt tgaagaggta
     4621 atcaaaagcc caagaaagat acaaacaagc gcaatcaaga ctacgacagc gctgctcatt
     4681 gaaaaccctg ctagaaaaac tatggcaaga aaccccaata atagaatttc tttcagcccg
     4741 ctttcatagg tcaaggccaa gctttctttt ttaaaccctt ttaaaacacc gctattgagt
     4801 tttatcaacg cgatgaacag caccaatatt acgccaatga tcaatgtggt ttgtgtatag
     4861 tcttcggaca gaagaacatt ggcatacaaa ccataaagac caaggcccgc aagggtggac
     4921 atccccaaaa cccatgcgaa tgaaaatcca tagaatttgc gcaataatga tgcttgtttt
     4981 gccaatgtaa attgtgggat caaacgaaca gacgaagcct gaaaaccaaa attccccagc
     5041 aaggcacaga gcatcgccga agagaaaata aaattaaaaa cgccatactg ttcaggagcc
     5101 agcatgcgag ccaaaagcag gtgcgacaaa aacccgcaga ccaagccaat ggcctgcact
     5161 gaaaacaggc ggacgagcag ttttacaaaa ccttgtggtg gttgttctat cgacatgttt
     5221 ccgcccagtt ttgtaaacac acaatggtcc agagataatg accgtaatcg gcctggccgg
     5281 aagcatgcgc atcccaataa aggcttacaa aatccggatc gatcaggctg tgttttttca
     5341 atgtactaac gcagagcata tcttctgccc agtctcttaa atcttcgcgc agccagcgcc
     5401 catgcggaat actaaagcct tgttttgggc ggtcaatcag agcatctgga atatatcgcc
     5461 gcaaaagctg cttgagagca tatttgccat gatgccccct gattttcatg tcatgcgaca
     5521 tgctccagcc atattcgatg acgcggtgat ccagtaaagg cgatcgtaat tccagaccat
     5581 gagccattga gcaacgatct gttttagtca acacatctcc ggccataaac atacgcgtat
     5641 caaacgccat catccgttca attggattgc ttacaggcaa agtctcaatc attgatccat
     5701 aggctgtgtc atcaatgttt tgagcgcgct gtccggtcac ccctgaccaa tagctatgca
     5761 aaagcgggta aagctcatca ggatcatcag ccgcgattaa cgcagaaatt ttacgacgct
     5821 gggcagcaga aaacaggccg ccagccaaag gaagtgcggt aagggcttgt ccaacagtgc
     5881 ggcgcaatcc attagggagc ttaagaacgg gcaatagttt ttgtgcacga aaataccagc
     5941 tataaccacc gaatgtttca tcgcctccat caccggtcag ggcgaccgtc agttcttttt
     6001 tggccagacg acacacatga tatgtgggaa tcgctgatgg atcgccaaaa ggctcatcaa
     6061 acaacgaatt gatatgccgg gcggtcttca tggcctcagc ggatgtaagc agcatttcgg
     6121 tatgctctgt tccgagatga gcagaaattt gcgcggcatg cggcgcttca ttgtagcgcg
     6181 aatcatcaaa cgcgattgta aaacttttga ccggctgatc ggcatgcttt tgcatcaacg
     6241 cggtaataag cgcagaatcc acgccgccag acaaaaaaac tcccaaagaa acatcggcaa
     6301 tcatccgttc tttaatggct tgggtgagaa tatcctccaa accgttaagc tgagagtgtt
     6361 cggctgcact ttgacgctct tgaccggtaa cagattcaaa gtgccaatac gtttcagcat
     6421 gttcctgcgg tgtgaacgga tgaataaaat cttcaagggg gcgtgtaata aactgtgcgg
     6481 gtggtaattt ccagatattt tcatagattg agtgaggatc aggaatatag cgccactgca
     6541 gataaagctg aatggctttg ttattgatgt ccggttttcg aaaagcctgt accgccgtaa
     6601 acgctttaag ttcagaagca aatgcaaaaa cgttcacccc tttatcccaa ccgaagtaga
     6661 gcggcttttt gccgcagcga tctcgcgcca aggtcaatgt gcgctcttta caatcaaaca
     6721 gggccagagc aaacataccg gcaactcgct taagtgtgtc tttgatgccc caatgcgcaa
     6781 atccggccag cagaacttcg gtgtctgaat gaccttgaaa gccgcagcca aatttttcca
     6841 attcgtttct taaagcttgg aagttataga tttcaccatt atacgaaatg acataacgcc
     6901 cagatgaaga gcgcattggc tgtgcgccgg ctttggaaag atcaattaca cttaagcgcc
     6961 ggtgagcaaa agccagccgc ttttcctgag gctctatcca catgccgtgg tcatcaggac
     7021 cgcggtgtgt gagcgcgtcg gccattgcct gaacgacatg ctcaaggttt tctgttttct
     7081 ggatttgaaa atatccggca attccacaca ttcaggacgc ttactcacaa gaggcgggtt
     7141 aagaagacga acgggcattc gacttttttg ctcaatcttc actataaaac cgattacgct
     7201 tcattgtaag tatagaattc tctatactat tgagagttct tatactggct gttctagctg
     7261 gcttgtttct gaataatgcg tatagatcat gatgtttggc atctcaaagc aatcttgttt
     7321 tatgttgata ttcacatgga tatcgaccgt atgtgtattg ggcataacac cattgacagt
     7381 ttttgcgcaa gaatctctgg aatcagaacc atttgctttt cacgcagaac cttcaccgga
     7441 tctcagcagc gcactggcag atccgtttac ctatcaaggc gaacctattg tttttaacgg
     7501 ttttcgtctg tggccaaatt tgacgttaga acaacgctat accgacaatc ttttggcaac
     7561 ggagaacaac acgcaaagtg atttcatcac ggtattgaaa ccggaacttg aaatctggaa
     7621 aagtctaggg cgccatgatt tttcattgtt gatgaacagt gaaattcttc agcactggga
     7681 tcgttcacaa gataatgtcg agaattacaa tgtcgcgttc aatgctgatc tggaagcgcg
     7741 acaaggcctg aacatcccga ttaaactgct ttaccgggat ggacatttgc ggcgcgccta
     7801 tcagcgccgc tccaacgccg accagattgt aaaaactccc ctgaatgtta agcgatggag
     7861 ggccgaaagc ggttttatat acaaacctaa tcgcttaatg cttagccttt taggcgatta
     7921 tagtcaggtt cgcctaggca atgaagagtt aatcaacggc gggtttttaa tccgtgataa
     7981 ccgcgatgtg aatatcacac aggcaaccgg taaggtctcc tacgatttga aaaccggcct
     8041 ttctcctttc gtgcagacat cctatacaaa ggaaaattat attaatgaaa cgcccggtgc
     8101 ggtgagccga aacaatgact cgctgcgaat tctcgcgggc gcggatttca attttaaggg
     8161 gttgatcgcc gggtttatcg gtgcgggtat agaccgacgt tcttatgatg atgcaaccgt
     8221 tggcaatacc ggtgatgtat tgctggatgc tgagatatac tgggacccga ttcctaaaac
     8281 ccgccttcga tttgatgcac gccgtgagac aacagaagat aatttattaa ttcaacaact
     8341 gacagaaacg gcttttggat tagggataga tcacgagtta cgacaaaatc tttttgctcg
     8401 cttggctgcg cactacggat atgatgaatt catggataca aaccgtcagg ataaaacact
     8461 ggatagtcat tttgagcttt taaaaatcat tgggccgcat ttacaactgg gagcaggcta
     8521 tcaatatttg acaaaagata gttctgttcc ggggttggat atggaccaaa gcgttgtgtt
     8581 tttaagaata aaaacagcgt tataaaccaa gccgttcttt tgttttttca tagatttcaa
     8641 tatcaccctg caaacgctct aacaacgctt tatgaaagtc gctattttta tcaggaacct
     8701 ttttctgctc ttgggatgca gggcttttat tacgcttaaa aaagattggt gttcttccca
     8761 gtttggcctg catgcgggct ttaaaattgt ctatattttc caaacaccca accacatcta
     8821 tttgctcaag cgtttcaatc gcctgcttaa catcctgtga tctgggcgtt tcatacatat
     8881 cttcagaacg agaaaaaaag tttacaaaag agcgtgcgtt catcataccg ttacggcttt
     8941 ctagatactc ttcgatagac aaatcggttt tacgatattc gtgatctttg tgacgattcc
     9001 aaaaatactc cgaataccag cgctgcaacg gatctcgcag caaggtgaca aaggtccact
     9061 catccttatg ggcggcatga aaatcatgat taaaaggata atggccgcat ataagcggag
     9121 aatctccttt ttccgcataa taactcaaca attttcgtcg gaaagcgtac tgacattcgt
     9181 tatgcatttc agccgaatag gaagcagctc tcgcattaag tgcgccagaa aaaccttcac
     9241 gcccataccg cagcaacctt aaggggtaat atgtaatcgc tagccccgtt atgattgctg
     9301 ttccgccaca tttggggacg tgatgaaaaa taatcttagg ctgttttatc ataagagagt
     9361 aatatagtct gtggtttgat caaaccatat cagtcttaat tccaacgtgc agcataaaaa
     9421 attataccgt acaatgagag tactatattg ccacgacaac atctatcaac agtgtgaaag
     9481 tggcaccgtt tattcgcctg gtcagtttcc ttacgaatac tggaaaattt ttttagataa
     9541 ttttgatcat ctggtggtaa tcggtcgtgg cgcaggactg catgataaaa ttgatcaatt
     9601 aaatatcagt agcgggccac gtgtctcatt cgcgctattt cccaatatca atactccagt
     9661 aggacggtta aaatatgcaa gacgcgtaaa taaacgcctt gaacggattg ttgcagatgc
     9721 ggatgccgtt atcattcgag ccgtcagcga tatgggctgg ctggcgttta aacacgcact
     9781 gcgtatgaat aagccgattg cccttgaaat ggcagcctgc gcatgggata gcacgtggaa
     9841 tcacggcaat aagcttggca aaatatatgc gcctatccgt tatttgcatg accgcatcat
     9901 tgctcgacat gccgatttcg tcatgtatgt gagttatgtg tttttacaac ggcgttatcc
     9961 gacgaatggt gaggtcgccc atatatcaaa tgtgaggatc gacaaacctg acgaacaata
    10021 tgtagatcaa cgactgaagg ccataaaagt caaacaagac gaaatgacag ctccttacac
    10081 cattggtctt atcggcaacc tcgataaccg gattaagggt gttgatctga ctttgcgcgc
    10141 gctagccaaa gttgaagaag aaaaaccggg atgttttata tttcatcatc ttggcccggg
    10201 tgacccagaa cctcatatgg ccttggcaca cgcgctagga attagtgaac gaattcgttt
    10261 tgacggcatg attaaaagcg gtgaaggcgt catgaagtgg ttaaggaaag tcgatctcta
    10321 tttacaacca tcgtttcagg aagggattcc gcgcgcaacc atcgaggcaa tgagtacggc
    10381 ttgtccgatt attgcgtcta ctgctggcgg aattccagaa ctaataaaag aagagtggtt
    10441 aatcaaaccc ggagatgttg ataatctggc ccgcttaatc ctcaagatgc ttgacaatcc
    10501 taaatttcag tacgaagcag gactgaataa ctatattgcc agccttgact atacgaacga
    10561 tattcttgta cccagacggc aagggttctg gggtgctttt gcaaaatttg ccagagaaaa
    10621 agcgcaataa tgtagaaaga aaaaacccag ataaatcaat gcaatccttt ccttgtgaac
    10681 acatggtgat ttattgtatt gttagaaagg ttgaatttct tccatgggtc cgagtttttt
    10741 aactaaatat caggtggttt atgtacaaca cgaagcttat agtagtgtcc gctgtgctgt
    10801 gccttgcaat cttggcgggg tgtgcccgtg ggacattagc tgatatacaa actcaaaata
    10861 gttccctgag caatgaacgc catgagcaaa accaaccgat ggcaacagaa aaagttgatg
    10921 cgatcgcaaa tcccatcgcg attgaaccca aaaaaacctt taacgatagc tgccacgaaa
    10981 aggaaaaaaa tgatgcgtat cgcttaggag ctgaagatcg tctggatatc ctagtttacg
    11041 gagaaacgga gctatctggc aattatactc ttgatacgac agggcatata tctatgcctc
    11101 ttatcggtga cgttcttttg ggcggatgta atatccgtca ggctcaggaa attattaaaa
    11161 acctttatgc tgacggatat ctcgttgatc ccagcatttc ggttgaagtt tctaattttc
    11221 gaccatttta cattttgggc gaagttaaaa gcccaggcaa atacgattat gtcgcaggaa
    11281 tgaatgttct gaaagctgcg gcaattgcgg ggggctttac gtatagggca aaccgtaaag
    11341 tcgcaaaagt tttacgttat gactcatacg gagaaccggt ttaccaccaa caaatcttag
    11401 aagatgaggt gcaaccggga gacgttatca cgatccccga gaaattattt tagcaatcaa
    11461 atggcagaga caacgcaaaa ttactatcga tcaaaagaac cgggcaatta tgccggttct
    11521 gatctcgatt tgtcgacgtt gtttcgtgtt atgtggcgac gaaaaatctc aattttattt
    11581 tttttgatcc ttggggtgac ggttactttt gtatcattat cttatatgaa gcctttatat
    11641 acatcacagg cagtgatttt gatccagaat aatgctggaa aactggctat aaaagatctc
    11701 tctcagttaa ttaagtccaa atctttgaat actgattttg ttttaaccga aattgagatc
    11761 ataaaatccc ggaacatggt ttcccaagtc gttaagcgtt tagggttaat tgatgaccct
    11821 gaactttcta atcatacgca tgtgccacag aatgatcaga atttcaaggc gttgttagta
    11881 gacggtacaa cttttgaatc tcttccaccg gaaactcttg atgccaaggt ttctatgact
    11941 atcaataaat ttcttgagcg cctgcgtgtc gtttcgatcc cggggtcgac agccgtacaa
    12001 atcaacttta cttccgaaaa cccttataaa tcagcaatca ttactaacgc aatcgttgat
    12061 atttacacta atcaaaaaat cagtgagcag acccggctac aacaaaggtt gtcggagtgg
    12121 tttgatgaac gtctacacaa tttgcgtgaa caggcacttg agaccgatag aaagatgcag
    12181 aaatacaaaa ttgaacatga actgaccgaa gaccaaacgg atattatttc aaccaaccat
    12241 ttgaataaat taaatgaaca gctgacggcg gcccaactca aaaaatctga agttgcagtg
    12301 accttacaac aactgaataa accagatgac tttgaaaata tagaagcatt aacgtcaaat
    12361 gatgtgaatt ttggaattct taaaaatttg cgccttgaag aactgcgcct aaaaaccgag
    12421 atttctgatc tttctaaccg atatggccct aaacatccgg ttatcaaaaa gaagaaatca
    12481 gagattgata ctatacagaa gtctttgagg agcgaagttc aggagataaa aaacgccctg
    12541 aatacagaaa taaaaattgc cgatgctaaa gcgaaagatc tagaacgaca aattcgtagt
    12601 aatataaaaa atatttcaaa taatgctgat acattgatga ttctgcgtga gttggaacga
    12661 gaagcggatt cagcggaact tgcccttaga acatttttag agacatacaa gaatattgcc
    12721 tggcaagata tgcttccggg atccggcgta caggtgattt cagatgcgag cgttccaacg
    12781 cacccatctt ttcccaacag ccccctattt ttactgttaa gcgctttcat ttcactgttt
    12841 ctagggatgt cttttgcaat tttcagggag catgtcgcta aaaattagag cgttgcatat
    12901 ggcatttatt agcccgattg ggctttattt gtgcttttaa agttaacatt ttctaatgtt
    12961 ctgcaatgtt ttaaattttc tatttatttt cagtgcgtta aatggataaa attttatcta
    13021 aatctaagat gtttttaatt gaaatttgtt atcctgaaaa ctgaactaat aattgaggct
    13081 gggttgtggc aggctttttt ttaacattaa caacaagtgt gttttttagt gccgatgcgc
    13141 caaacataga agttttggtg aacggtgttg tttctgcgtc ctttattgaa accgaacttg
    13201 agggcagcgg tagcaccacc cgaacgctat atatcgatta tgtcggacta tctccttcag
    13261 atatcactgt acaaattaat gatgcctctg gtgaaaccgg gagatacctc gttttggaag
    13321 ccgttgcggt caacggtcag aatattcgta ataatacgct gaatgtaaat gatggcacac
    13381 cggttgacct tctaacaacg ctggaaagca tggattttga tacaaccaat acggattggt
    13441 tatttgggca aaccgaaccg cttttatctt attttggaac ttcgacttac accggaacag
    13501 gggcggatga tacgcaaaac ggcagccata ctgtaaatga tgtcatgaat atggggggtg
    13561 gtaatgatat cgcgaacggc aggaatggta atgatttcat gtttggtggc gccggcaacg
    13621 atcttttggt tggtgcggat ggccatgacg ttttagtcgg cggcgatgga aatgacgaac
    13681 tgcgtggcca gcttggtaat gacatcttgt atggagggct tggcgatgac gttctctacg
    13741 gcaatgaagg aaacgacacg ctgaatggtg gtgcaggtaa tgacctcatc aatggcttcc
    13801 acgacgatga tgtaatttat ggcgaagccg gtatggataa gctttatgga ggggatggca
    13861 atgacactat ctttggtggc gatgacaaag acttcattta tggcgaagcc ggtatggata
    13921 acctttccgg gggtgaaggg gacgacatta tcgaaggtgg cgatgataat gacattattg
    13981 ccggcgatgg cggaaatgac aggatctggg gtgatgctgg tgatgacaat ataaatggcg
    14041 gcgctggact ggataccatc tatggtggtc ttggtatcga tacaatcagt ggcggtgatg
    14101 ataatgaccg cattgaaggc aatgatggag acgatatcct caatggcgat gccggccagg
    14161 actacatata tggcggcttg ggcaatgata ttatagatgg cggcaccgaa gccgatattc
    14221 tgtatggtga agagggtgat gacattatta atggcggaga tcagaacgat acgctctatg
    14281 gaggggacgg catagacacg atggacggag gggctggttc ggatcgtata tacggtgaag
    14341 cggatgacga tactatttat gggcgtggcg gcgacgacat tatcgaaggc ggtgacggta
    14401 acgatacaat ttacggcaat ggtcagaacg atattcttcg cggggacgcc ggtgatgact
    14461 atctaaaggg cggaaccgga gacgataacc tttacggcgg cgatggaaat gacgatctgc
    14521 gcggggatga cggaaatgat cgtttgaaag gcggcgccgg caacgatcaa ttgcgtggcg
    14581 gcgatggcga tgatgtactc gctggtgaag atggagacga tattctctat ggccagggtg
    14641 gaaatgacaa gctctacggt caggcgggta atgatgattt acgtggcggc gatggcgatg
    14701 atcacctctt tggacgcgaa ggcatcgaca ttcttcaagg gaataatgga aacgacacct
    14761 tggtctatga cggagtggac acgctggatg gcggcgcaga tatagacact attttagttg
    14821 caggttatga cacagtaacc atagatttcg ccagcggcct gatttccaat atggaagccg
    14881 tcaatatggc caacacgaac ggcattgctg cagcaaataa ccttcagatc agcatggcgc
    14941 agattgcggc cagttcagat acaggaaaca tgacaatcac cggggacgtt ggtctggacc
    15001 aagtcaattt taccctgact ggctctgaaa cccgcggtgc agacgttaac attgatggca
    15061 tcgattatgc tcagttcaat gatggcggaa cgacagctta tatacaactt ggattgattt
    15121 ataacggatc agttctaaca gcttcaggcg gcggtagcag caatacaccg accccaggag
    15181 atgatgtact cacgggaaca tctggggaag atacaatcag cgcgcttgct ggtaacgata
    15241 tcattattgg cgaagctggc aatgatacac tcagcggaga tgagggaaat gataacatct
    15301 acggcggtga aggaaacgac acgctaaatg gtggtgacgg taacgatata ttgcatgcat
    15361 cactttccgg ctccatcacg ccaagtacag atccggccac ggtcgtcgct aataatcccg
    15421 gcgtcgttta cagctctgaa accggaaact tctatcagtt tgtgacggca agcgcgaact
    15481 atacaactgc cgctgccact gccgctgccg caacgcttga gggcatttcc ggtcatttag
    15541 taaccattac aactgttgcc gaaaacactt tcatagaaaa cctgacggga acaaattttg
    15601 cctggatgga cggaacggat tctgtaaccg aaggcacctt tgtctggaca gctggcccag
    15661 atgcgggaac gcagttctgg aatgatatcg gcaactacac caacttttat caaggatcgc
    15721 caacaactaa ctcaaatgcc aatgataacg tgattttcct tggaacaaca tatagcggta
    15781 cgtggtatgc ttacacaggc acctatgcca caaactacct tattgagtgg gaaggcagcg
    15841 atgttattca ggaaataatt gtcggatttg acagccgtaa tgaaacaaac tatctcaatg
    15901 gcggcgccgg tgacgatcaa ctttatgggt cgatgggccg tgatattctc gtcggtggga
    15961 caggtgcaga tattctcgaa ggcggcgctg gtaatgacaa actctacgca gatggcctgt
    16021 ccgacgcgga aattcaagcc attttaacgg caaatcctgg cgtgacatat tcagaagaaa
    16081 ccggaaactt ttatcagttt gtctctggtt catttaatta cgcgaatgcg ctcgcaggtg
    16141 catcttcaac cacaattaac ggcatagccg gccatctagc aacgattaca tcagcagaag
    16201 aaaatagctt tataaacggt attgttggcg caaattttgt ctggatcgat ggctcggatg
    16261 cggcgacaga aggtaccttt gtctggacaa ctggcccaga tgcgggaacg cagttctgga
    16321 atgatatcgg caactacgag aactggtatc agaactcacc gacaagcaat acaaatgcca
    16381 acgaccacgt tgtatttttg ggacaaaatt ataacggcca gtggtacata ttcggcgaca
    16441 acaccaacct aaatggttac gtgattgaat gggaaacatc aagttttgga acagatacat
    16501 cggctaattc tctcaatggc ggttacggtg cggatgacct ttatgcgtct ggcgggattg
    16561 acacgtttat ctttgaagct gccagcgctt tttcagatat tgataccatt cacgattttg
    16621 ataacactgc agatattctc gacatttcgg atattttaag tggaataaat gttgatgcaa
    16681 gcaatgttgc agattatgtc tcagttgatg aattaaccgg tgttcgcgtt gacgtgaacg
    16741 gcaccggtac attcggtgcg gggacacaaa tagcatcatt cagtagtgct gttggggtcg
    16801 acgacgctct aaccatgttc aacaacggtg atctgatcgt ctaaggtaga ttctatagta
    16861 ccttttaggc ttatagtacc ggggcaaaaa tcaaatttct gtaaagtggc tgtgagtggt
    16921 gatcgcgcag ggattcgaac cctggaccta ctgattaaaa gtcagttgct ctaccagctg
    16981 agctacgcga tctcacgaat taacccctta gggctacaaa agattgtgga aacatatgga
    17041 tttagaaggc gtacgtcaag ctttctttgg ggagggtttc gaaaatttcc gggagatggc
    17101 ctttgcaacc tgaggtgtga cgaattcgtt gatgtcgcca cccaaagagg ctatttcctt
    17161 gacaaaactg gcggaaatga actgccattt atcagcggcc atcaagaaca cggtttcaat
    17221 atcggggtcg agcttggcgt tcatgccggt gagctggaat tcatattcga agtctgatac
    17281 ggcgcgcagg ccgcggaaaa tacagtttgc ttgcatttcg ctggcaaaat ggaccagcag
    17341 gttgttgaag gagcggacct caatctcaca gcctttttcc tgcatgttct caatgtcggt
    17401 gcgaaccatc tcaacgcgct cctcataggt gaaaagcggg cctttgcggg cgttatcggc
    17461 aacgccgatg atgagcttgt ccaccatgcg cgaggcgcgg cggatcaggt gcagatgccc
    17521 cttggtcacg gggtcaaacg tgccgggata tacgccgatg aggtatttgc cgttattctg
    17581 cgtcatcgct ctcggcagct tcgtccgcag ggggctgagt ttgggcgctt tcggtttttt
    17641 catcaccctc ttccggcaaa acttcgccct caagctcatc ctcatcttca tcatcatctt
    17701 cctggaccag ccaggcgacg gagacgacgt tttcatcgtc tccgaccttg aagatggtga
    17761 caccctgggc gctgcgcccc gtcacacgga tattttcaac cggcgtgcgg atcagcttgc
    17821 ctttatcggt gaccagcatg atctggtggc tatcggttac cgggaaagtg gcgacgactt
    17881 cgccaccgtt tttcttggtt agagacatgt tggccacgcc ttggccgccg cgcccggagg
    17941 tgcggtattc gtaggcagag gtgcgtttgc cgaaaccttt attggagacg gtgaggacga
    18001 tttgctcttt cttcagaagc tcgcggaagc gctcttcgga aagttccagc gtggtttcct
    18061 catattcgaa gtcttcatca tcgctgccga cgatctggct ggcggctttt aggtaggcgt
    18121 tacgctcatc gggtgtaatg tcaacgtgtt tgagaatcga catggagata acctcatcgc
    18181 ctttttgggc gagcttaacc ccgcgtacgc cggtggaatt gcgcccggca aagacgcgaa
    18241 tatcggtgac ggggaaacgg atggctttgc ccattttggt ggccagcatc acgtcttcat
    18301 cctcggtaca tattttgacc gaaacaaggc tttcctcctc gcccagtttc atggcgatca
    18361 ggccattgga gcggatgttg gcgaagtctg taagtttgtt gcgtcggacc gatccgtgcg
    18421 aggtggcaaa catgatgtcg agatcatcaa taacatcaac atcttctggc atgcgcatat
    18481 aaacattcac cttttcatct ttttcaagcg gcagaaggtt gatgatcgct ttgcctttgg
    18541 atgtcggaga gcccagcggt aattggtaaa ccttcatttt gtgcacgata ccgcgcgaag
    18601 tgaaaaacag aatcggcgtg tgggtgcttg cgacaaacag gtcggagaca aagtcctcgt
    18661 ctttcgtact catcccggcg cggcccttgc cgccgcggcg ctgggcgcga taggtgtcga
    18721 gcggtacacg cttcacatag ccctcatgcg taatggtgac aaccatatct tcgcgctgga
    18781 taagatcttc catatccatt tcgaactcgg cttcgcaaag ctctgtgcgg cggggggtat
    18841 cgaaacgatc cttgatctct ttaagctctt cgaccagcac ttcgagcaga cgctcgcggt
    18901 ctgcgagaat ttcgagatat tcggcgattt tatcggtgat ttccttaagc tcggcagcga
    18961 ttttatcacg ctcgaggcct gttaaccggt gcaggcgtag ctcgagaata gccttggctt
    19021 gcgcctcgga catctgataa gtgccgtcat cattcagtgg gtgttcagga tcgtcgatca
    19081 actcaataag cggggcaacg tctttggcgg gccaactttt cgccatcagc ccttcgcggg
    19141 cggtagtcgg gtccggcgcg ccgcggatca gagcgatgac ctcatcgata ttggcgacag
    19201 ccacggccaa cccagccaaa atatgggcgc ggtcacgggc tttaccgagt tcgtatatcg
    19261 tgcggcgcgt gatgacttcc tcgcggaact ttacgaaggc cttgatcatg tcacggatta
    19321 acatcatctg cggcttgccg ttatgcagcg cgaccatgtt gcaggcgaaa ttgctttgca
    19381 aggcggtgtg cttaaagagc tgattgagca cgacatcggc aatcacgccg actttcaact
    19441 caattacaat gcgcacgccc tcgcggtctg attcatcgcg aatttcagcg atgtcttcga
    19501 cgattttttc gcgaccgact tcgccgaggc gctcaagcag cttgcctttg ttgacctgat
    19561 acggaatttc atgaacgatg atcgcttcgc gcttcccaat ttcttcaatc gaggtttttg
    19621 agcgcatgac gaccgagccg ttgccggtgt gataggcttt ataaataccg tttttgccca
    19681 taattttagc gccagtcgga aaatccgggc cggggatgat ttcgttgatt tcttcggtag
    19741 tgatgttcgg gttatcaacc atggctacgc aagcatctat aacctcgcca agattgtgcg
    19801 gcgggatgtt ggtggccatc cccacggcaa ttcctcctgc cccgttgacg agcaggtttg
    19861 gcacgcgcga aggtaaaacg ctaggttcgg taagagattc atcgtagttg ggacgaaaat
    19921 cgacggtttc tttttctatg tcctgcagca ggacttcggc agctttgtcc atccgtgctt
    19981 cggtgtagcg catcgccgca gggctatcgc cgtccataga gccaaaattc ccttgcccgt
    20041 cgatcagcgg caaacgcagg gaccaaggct gggccatccg gaccatagaa tcataaatag
    20101 ccgcatcgcc gtgcgggtga tatttaccca taacatcccc gacgacgcgg gcggattttt
    20161 tgtagggttt atcggaggta taacctcctt cgcgcatggc gtagaggata cgccggtgga
    20221 cgggcttcaa tccatcacgc acatcgggca aagcgcggga tacgatcaca ctcatggcat
    20281 aatcgaggta agattgcttc atttcctctt caagggagat ggtgggaatt tgatcgtctt
    20341 caataacgga ggaatcgctc atggataaac cttataaact cttattttta ggtggttctg
    20401 atcgtacata tttttctagc aaatatgaca gcgcaaagat agcaaaacct gtcatcattc
    20461 caggaactaa atccatcaat tgtgcctgca atccaagacc gtgacgccag tacacggcca
    20521 cgccaaatcc ggcgaccatc atggtgacgg cgagacgctg ggtcggtttt tctccgaaca
    20581 gtatcaaaac gaccaaaggc gctacagctg aagccattcc accccaggcc aaaaccacca
    20641 gtgagagcac attttggctg gccagcaggg cgatcgctac gatcatgcag atcaccgcaa
    20701 ctgtgccaag acgcgccagc gcgtaggagt tacggagctg tggaaagaca tgctgggtca
    20761 atgccgagga tgcggaaagc aagagcgagt cggctgtaga catcgttgca gaaaacagcc
    20821 ccgcaaccat gaagccgacg aaaacgggcg gcagaaggtc ttcccaaaga gcaggaatcg
    20881 ataattcagg gtcaaaaccg ccttcgattt caggaatcat aacgcgagca ataaagccga
    20941 ccagcgtagc cagggctaaa acgctccagc gccagctcag atagatgcgc cgggcggtaa
    21001 gcaaatcttc atcggagcgc gcaaccatat ggcgcaccat caactggggt tggcccagca
    21061 cccctacacc aaaggccagc cagcctataa aaacggaaat aaatttggtt tgcgccggat
    21121 tgaccagcgc cgggtcgatg gctttgagtt gtctgtaaag ctctccaaaa cctccgatct
    21181 gctccattgc gacccagacc agcaccatga gagaaagtat gatgatgatc gcctgcaccg
    21241 catcggtcca gatcgaggcg cgaatgcctc ctgcccagca ataggccaga agcacgaacg
    21301 cgccgacaaa aacaaagatg ttgtagtccc agtcaaaaag tgcttcgccg atcttggcac
    21361 cggccacaag ttgggcgcta caataggtcc ccagaaaaac cacggtgagc atagcggtaa
    21421 taacagtgac gatttggaag tcgcgccctg ccaatttacc aaccagtgac ggataagtat
    21481 gggctttggt ttcaattgct ttgcggcgta ttttgttgcc ggcgataatc cagcccaaat
    21541 gatcgccgat cgttagcccg acaaccgcat atacggctgt gagaccggaa ttgtaagcca
    21601 gcccggcaaa gccgatgaag gttgcgccgc tggagatcgt tgcaccgtag gacaaagcca
    21661 ccagccatgc aggaatggag cgcgaagcca ggaaataatc ttcgatatta ttttttcgaa
    21721 aaaaggatga ggccagaccg acgcctgtga aaaagataaa aaacaccaga aagctaatca
    21781 gcatggcatc catgatcgag tatcctttgg tttacgggca accctacggg ctttgggttt
    21841 ccgtacgaaa tctcacaagc gcctgatcga gcatacgcac gatttccggc gcgattcgtg
    21901 cattgagctg ttcttgttta tcactggccg atcgcgcttc aggctggtta taataggcca
    21961 ccatggcctc aagttctttc agggtaaaaa tttcagcgta ggaatcgacg gtgatttttt
    22021 ccagcgcctt ggggttcatg atttttagca gagcagagcg cacgacttct ttgtcatttt
    22081 ccgatgcgcc agccatggcc cgttcaagat atgcattgat ggcggtttcg agttgctgtt
    22141 tggccggacg aatttcgagc attttttctg ccaatttcag gcgctgggga aaatcatcgc
    22201 cctgtgcgaa agctggcgca cagaacatca aaatactaac gaaaagcgtt aaacgaagca
    22261 taccctaaaa cggaatttcg tcttcgagtt catcgaccgg agcggcggct tgagcctgtg
    22321 tcggtgcgcc ttgggagaac ccgccgccct ggctatatcc ggcattatcg ttaccgctgc
    22381 gtccatcaag catcacgagc ttggcatcat agccgcgcaa caccacttcg gtggtgtatt
    22441 ttttctggcc gtcctgttcc caactgcgcg tttgaagctg gccttccaga taaattttag
    22501 aacctttttt taggtagttc ttacaaacat tgaccagacc gggagagaag atcacaacgc
    22561 ggtgccattc ggttttttct ttacgctcgc cagaggcttt atctttccaa gtttcggatg
    22621 tagcaataga gaggttacaa acttcgtcac cactttgcat ggatcgcact tcggggtctg
    22681 cgccgagatt accgattaat gttactttgt ttaatgaacc agacacgttt ttactcctaa
    22741 caattaaatc ttaagcgccc actatagcgt ccgggcgtga ctttgcaaat aagtattgga
    22801 cttctgcgcg gccttatccc atataaggca ttatgagctt gaaaactatt tctatccgcg
    22861 gtgcgcggga acataactta aaggatatca atgtcgatat gccgcgtgac aagttgattg
    22921 tcattacggg gttgtcgggg tcggggaagt cctctctcgc ctttgacacg atttatgcgg
    22981 aaggccagcg gcgttacgtt gagtctttga gcgcctatgc gcggcaattt ctggaactga
    23041 tgcaaaagcc ggatgtcgat agcattgaag ggctgtctcc ggcgatttcg attgagcaga
    23101 aaaccacgag ccgcaacccg cgctcaaccg tgggaaccgt gacggagatt tacgattaca
    23161 tgcgcctgct gtgggcgcgc gtgggcgtac cctactctcc ggcaacgggc aaaccaatca
    23221 ccagtcaaac agtcagcgag attgtcgatc gcatcatggc gatgggcgat gggacaaagc
    23281 tctatctgct agcccccatc gtgcgtgggc gcaagggcga ataccgcaag gaatttaaag
    23341 agctacaggc gaagggcttt cagcgcgtga aggttgacgg cacgctgtat gaaatcggcg
    23401 atgtgccgac gctagataaa aagctcaagc atgatattga ggtggtggtg gaccgattgg
    23461 tcgtgcgcga agatatgggc aaccgactgg cggattctgt ggagacggct ctgcgactgg
    23521 ccgacgggct ggttctggtc gagaatgcgg aagacggaac tcaagagctg ttttccgaga
    23581 aatatgcctg ccctgtttcg ggttttacga tccccgaaat tgagccgcgg ctgttttcgt
    23641 ttaactcgcc gcacggagct tgtccaacct gtgacgggct gggcaagcgg atgtatatgg
    23701 atgaggctct ggtggtgccg gatggctcga agtctttgtt gaaaggcgcg attgagccat
    23761 ggtcgaagag ctttgcgccg ttttatatgc aggcgctcga aggcgtggct gcgcattacg
    23821 ggtttagcgc ggcggagaag tgggacaaac tgcctcgcaa atatcaggag attatcctgt
    23881 atgggtccgg tgatgaaccg attgcgatta cttacaacac caagaccgag agcacgtgga
    23941 aatcaaacaa gccctttgag ggggtgatcc cctctttaga acggcggatg attgaaacag
    24001 attcgaattc agcacgggaa gaactggcga agtatcaggc aagcgcgccg tgtgaggcgt
    24061 gcgagggaca ccggttgaag cccgaagcgc tggcggtgaa gattgacggg aagaatatct
    24121 ccgaggtcgg aacctatagc attgaagagg ctggcgagtg gtttgaacgt ctttctcatc
    24181 ccgtcattgc gagcgaagcg aagaaatcta gaaagcaaaa gaatggattg ccgcggcctg
    24241 ctgacgcaga cctcgcaatg acgaatgaag aaatcctcac cccccaacaa taccaaattg
    24301 ctgaaaagat cctgaaagaa attaatgaac gtctgaattt tctgcgcaat gtgggactgg
    24361 attatctcaa cctgtcgcgc aattccggaa cgctttcggg tggagaaagc cagcgcatcc
    24421 gcctcgcctc gcagattggt tcggggctaa cgggtgtttt atatgtcctg gacgagccgt
    24481 ccatcggcct gcaccagcgc gacaataatc gtttgctgga aacgctgacg cgcctgcgcg
    24541 atatg
//
LOCUS       DAOSWR010000011        24377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1321_length_24377_cov_5.527013, whole genome shotgun sequence.
ACCESSION   DAOSWR010000011 DAOSWR010000000
VERSION     DAOSWR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 24377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24377
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_24377_cov_5.527013"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..1727)
                     /gene="ahcY"
                     /locus_tag="PK513_01720"
     CDS             complement(411..1727)
                     /gene="ahcY"
                     /locus_tag="PK513_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO81201.1"
                     /translation="MSAVPKSLENDYWVADISLAEWGRKEIELAEIPGLMALREEYGT
                     KQPLKDARIAGCLHMTIQTAVLIETLQALGATVRWSSCNIFSTQDHAAAAIAATGTPV
                     FAKKGMNDEEFWWAIDQTIEGPDGQGGIWTPNMILDDGGDLTVRMHESFPKLLDDVKG
                     ISEETTTGVLRLYEMEKKGKLRCPAINVNDSVTKSKFDNKYGCRESLVDAIRRATDVM
                     MAGKVAMVCGYGDVGKGSAESLRKAGCRVLVSEIDPICALQAAMEGYEVVTMENAAPR
                     ADIFCTTTGNKDIITVDHMREMKDNAIVCNIGHFDNEIQVEPLRNMKWTNIKPQVDQI
                     EFPDGKRILLLAEGRLVNLGCATGHPSFVMSASFTNQTLAQIELWQNHSAYDNKVYVL
                     PKHLDEKVAALHLEKVGAQLSKLTDAQAEYIGVSPNGPFKKDDYRY"
     gene            1784..2170
                     /locus_tag="PK513_01725"
     CDS             1784..2170
                     /locus_tag="PK513_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81202.1"
                     /translation="MKFNEESSKPPFIYKRNPLVEHAWRIFALKEGKADYEPVGEYTV
                     LDLDEPSDLTEKKMANLIAILNGRRELQQLGELTKSRLLFTQVPEEEGAINKKLIFRT
                     YDGDGISKENAILVIEKGVFKDGGQT"
     gene            2154..2279
                     /locus_tag="PK513_01730"
     CDS             2154..2279
                     /locus_tag="PK513_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81203.1"
                     /translation="MAGKPEFSTVIQQFLSAASGPVETVSPAHNLEKPMGLDLRR"
     gene            complement(2429..3274)
                     /gene="rapZ"
                     /locus_tag="PK513_01735"
     CDS             complement(2429..3274)
                     /gene="rapZ"
                     /locus_tag="PK513_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOO81204.1"
                     /translation="MNEQTNQTPLIITGLSGAGLSSVLKNLEDLGYEVFDNFPLSLVP
                     ALIKDSPHPSTLAISIDTRTRGFAPDKVLETVKKIKALLIYITCDDAVLQKRFTETRR
                     RHPLADDAPVSIGIARERDLLCQIKDKADLTIDSSDLSVHDVRHILEGHFGSRHEKTL
                     SISLISFGFRHGLPREADIVMDVRFLKNPHWNKTLKPKTGLDAAVGDYIKTDPDFSGF
                     IERFQTLLEPLIPRYMEEGKHYLTIAIGCTGGRHRSVFVTEEINRWLSKCGLATHVTH
                     RDLPT"
     gene            complement(3271..5028)
                     /locus_tag="PK513_01740"
     CDS             complement(3271..5028)
                     /locus_tag="PK513_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO81205.1"
                     /translation="MASDTDTKKTENQNHNGDKARWFRLAPVRPDLPEFDHLLDFYWG
                     GPERRITGITLRIIAVNAITLLILMFGMLYLGQYQNNLINARLQTFETELNLISTSLS
                     EKEIPNTNLDIDANRVIFKNLEKLASRFAKTSRQRIRIFNTQGTLILDTRTLPDHDDR
                     LWALEDRKTQDFHSIKTLKKMMRFIIDLLPEHQSLPTYPENTEIPPGLIDALNKQITL
                     SAWQNKQGRIILSAAAPLLMNEKPSGAILLTHESTEIEQDISRVWIDVLKIFSGALAM
                     TILLSIYLSGVIARPLKKLANAAENVRRGQARSDDIPDLSHRHDEIGELSLALRQMTS
                     ALWNRMDSIERFAADVAHELKNPLTSLNSAIETASIVKKTSDRKKLMDIIKHDVQRLD
                     RLISDISNASRIDSELSREAFEKINLHTLLTTLLETYAANPLERSNGAEQDWTRSIQT
                     PKATIELHNTQPKNIFVWGLESRLAQVFQNLLSNALSFCPADSKITVNVTPLATKVHI
                     TIDDEGPGIPENKMKTIFERFYSERPQHEHYGQHSGLGLSICRQIIEAHGGQIFAENI
                     YGPEEKPRGARFTVILNKI"
     gene            complement(4997..5707)
                     /locus_tag="PK513_01745"
     CDS             complement(4997..5707)
                     /locus_tag="PK513_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO81206.1"
                     /translation="MSAKITLVDDDRNILTSVSMALEAEGYEITTFNDGEAGLKGILE
                     ETPDLIVLDIKMPRMNGLEVLEKLRETLTIPVIFLTSKDDEIDEILGLRMGADDYISK
                     PFSQRLLIERIRSLLRRHSLMQENGKAQKTQDATEKYTYGPLEMDDQRHLCTWNNEPV
                     NLTVTEYLLLKSLAQRPGIVRNRDQLIDMAYGENIYVDDRTIDSHIKRLRKKFKKTDP
                     DFNQIETLYGVGYRYKEN"
     gene            complement(5712..6998)
                     /locus_tag="PK513_01750"
     CDS             complement(5712..6998)
                     /locus_tag="PK513_01750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOO81207.1"
                     /translation="MYQSDLNHPEKSLEDKLHQLYRLNRNKTIDLSFRPPYLDLLEKL
                     GHPERNLPPVIHVAGTNGKGSTIAMLRAILESAGYKVHTYTSPHLIHFNERIILANQE
                     IDDQALETLIDETLELNEGGDLTFFEITTAIAFAAFARTPADICLLEVGMGGRLDCTN
                     VIENPLVTIITSIGLDHTEHLGNTYTAIAAEKAGIMKPKVPCIIAPQSNQALNAGVID
                     VFEETARRIKAALYQTEDVPKNLTCALIGPHQRANIAAALKTLELIKDRFPLTDEQIK
                     TGLAMAQWPARLQKLNTEDYNLSPDWQLYLDGGHNEDAGHAVAEQVAIWKKQDDRPLH
                     IILAMLQGKDARAYYAPLRPFVDTLSLITIPGEPKAQSPEDLQKTLSGGQIFENYQNA
                     LTHIEQTSLSPGLILITGSLYLAGHVLKDAGKSAHT"
     gene            complement(7014..7880)
                     /gene="accD"
                     /locus_tag="PK513_01755"
     CDS             complement(7014..7880)
                     /gene="accD"
                     /locus_tag="PK513_01755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOO81208.1"
                     /translation="MNWLTDFIRPRVRSLVGEQKEVPDNLWQQCPSCEGMLFHRDLAE
                     NMNVCYHCNHHLRITAKERLKIMFDAGKYETLGVPSVPHDPLKFKDRKKYADRLKETQ
                     SKTGLKDAITIAKGTIDGQKAVVAAFNFSFMGGSMGTAVGEGLIKAAQEAVKDHAAFI
                     AIPSSGGARMQEGMLSLMQMPRSIIAVEMVKEARLPYIVLLTDPTTGGVSASFAMVGD
                     IHLAEPGSMIGFAGRRVIEETVRETLPDDFQTAEYLQEHGMVDMVVNRKDLHETLGRI
                     LRLLMNTEKKAA"
     gene            complement(7958..8734)
                     /gene="trpA"
                     /locus_tag="PK513_01760"
     CDS             complement(7958..8734)
                     /gene="trpA"
                     /locus_tag="PK513_01760"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO81209.1"
                     /translation="MSRIEATFKALNRPALITFIMAGDPDAKTSLDVLKALPEAGADI
                     IELGMPFTDPMADGPTIQLAGQRALKSGANMKQTLQMVRDFRKGNNTTPIVLMGYFNP
                     ILQYGVEKFMNDASAAGVDGLIIVDLPPEEAGKAQEIAKTNNLDIIRLLTPTTTMGRL
                     PKILEGASGFLYYVSITGVTGAAQADLNKIKPHIEQIKTHTDLPIAIGFGIKTPQDAA
                     QMAALGDAIVVGSSIVQTIAEKRDASAVSEQVKALSDALS"
     gene            complement(8731..9201)
                     /locus_tag="PK513_01765"
     CDS             complement(8731..9201)
                     /locus_tag="PK513_01765"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOO81210.1"
                     /translation="MANYEVRAGLAEVEAFDVVGISTITDNQKGTDDINALWERFFSE
                     SIGQQILDKTDDAIYAVYSDYQGDHEAPYRLTIGYRVSNDACAIDMHRVRVQPQEYAV
                     MSAAGEQPKALIETWEAIWSSDIERSYTTDFEVYGPRFFEQGVNEVLVHVGVKS"
     gene            complement(9194..10423)
                     /gene="trpB"
                     /locus_tag="PK513_01770"
     CDS             complement(9194..10423)
                     /gene="trpB"
                     /locus_tag="PK513_01770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOO81211.1"
                     /translation="MSQKNLKTNSYRNQPDERGHFGPYGGRYVAETLMPLILEVEKAY
                     EAAKADPKFWEELENLQRNYVGRPSPLYFAGSTTEELGGAKIYFKRDELNHTGAHKIN
                     HCLGQILVAKRMGKTRIICETGAGQHGVATATVCALFNLPCVVFMGATDIERQAPNVF
                     RMKLLGAEVRAVKSGAGTLKDAMNEAMRDWVTNVHDTYYLIGTVAGPHPFPAMVRDFQ
                     SIIGTEVRKQIMEKEGRQPDHLVACIGGGSNAMGLFHPFLDDADVKMIGVEAGGHGVG
                     TGQHAASLTGGKPGVLHGMNSYFLQNDDGQIQEAHSISAGLDYPGIGPEHAWLFDQRR
                     VEYVSVTDDEAIEAFQTMARLEGILPALEPAHAFAHVMKRAPTLPKDDIIVMNLCGRG
                     DKDIFTIADKLGVKIHG"
     gene            complement(10430..11068)
                     /locus_tag="PK513_01775"
     CDS             complement(10430..11068)
                     /locus_tag="PK513_01775"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOO81212.1"
                     /translation="MTAVKICGLKDPENLSAATSAGARYVGFVFYPPSPRNIAADSAK
                     ELSLTLPTGVKAIGLFVNPTNEQLEHILGIVPLDMLQLHGSESPERVQQIKDKFAMPV
                     MKATKIREPADLETVPAYEETADWLLFDSRPEAATLPGGTGHSFDWSLLAGKTFSRPW
                     MLSGGLTPENVAEALSQLSPTAVDVSSGVESAPGVKDPAKIKAFIDAVKSAI"
     gene            complement(11065..11760)
                     /gene="pyrF"
                     /locus_tag="PK513_01780"
     CDS             complement(11065..11760)
                     /gene="pyrF"
                     /locus_tag="PK513_01780"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO81213.1"
                     /translation="MSSRPLIFCALDTADLEQARLWARTIGPVTGALKLGMEFINAHG
                     PAGIETVKNAAPDAALFIDLKFHDIPNTVAGAVRTICANFAPAYLNVHAAGGRKMMEA
                     AKEACTPQTKLLAVTILTSLSENELCKIGYKEGLPERVIQMARLAETSGLDGVVCSSH
                     EITLIRESCADSFETMVPGIRPAGSAKADQNRVMTPNEAVQKGATHLVIGRPITGAPD
                     PAKAAQEIMDSLK"
     gene            complement(11788..12147)
                     /locus_tag="PK513_01785"
     CDS             complement(11788..12147)
                     /locus_tag="PK513_01785"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOO81214.1"
                     /translation="MSFIRAIFGFIIAIALVAFAVSNRQNIALNYSPVHDPLEIPLYL
                     LTLLFMALGFIFGAFIMWMNTAPTRRSSRQQRKTIKALEKELANAQNKKTSPANAPPS
                     ELFRALPTSAAEKHHLA"
     gene            complement(12171..12494)
                     /locus_tag="PK513_01790"
     CDS             complement(12171..12494)
                     /locus_tag="PK513_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOO81215.1"
                     /translation="MTKSELIQRLAERSPHLYLRDIEKIVDTIFDEITQALVDGNRVE
                     LRGFGAFSVKHRDARIGRNPRTGESVHVEAKRLPFFKTGKGLRERLNDKTPSDDSSSS
                     EDSWY"
     gene            complement(12886..14739)
                     /gene="rpsA"
                     /locus_tag="PK513_01795"
     CDS             complement(12886..14739)
                     /gene="rpsA"
                     /locus_tag="PK513_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOO81216.1"
                     /translation="MASVAAKIQDKTDSKEFAALLEESLKGQENFTGSVVNGTIIAVT
                     DDFAIVDVGLKSEGRIPLQEFSRPGEDAEIKTGDVVEVFVERMENRDGESVLSREKAR
                     REEAWIELEKASEKEERVTGVIFGRVKGGFTVDLGGAVAFLPGSQVDIRPVRDVSPLM
                     DTPQPFLILKMDRARGNIVVSRRAILEESRAEARSDLMDNLKEGQVLQGVVKNITDYG
                     AFVDLGGVDGLLHVTDISWKRVNHPSESLQVGQTIDVQVIRFNEENQRISLGMKQLED
                     DPWKDIDTRFPIGTRLKGRITNIADYGAFVELEDGIEGLVHVSEMSWTKKNVHPGKIV
                     STSEEVEVMILEVDEEKRRISLGLKQCQDNPWGAYAASHKEGEEFEGEVRNVTEFGLF
                     VALDDEIDGMVHLSDISWEDESEEALKAYKTGDKIKVKILEIDPDKERVALGIKQLTE
                     DPFEGAMDGVKKGAVVTCTVTEITSGGIEVSVNDNIKGFIKKADLSRERSEQRPDRFA
                     VGEKVDAKITSMDKKSRKVALSIKAREVDEDKQAMKEFGSTESGASLGDILGAALEEK
                     KGKEAKEEKPKKTPAKKAAAKKTTSKKKDDEAKAKSADKKADKKADKKKED"
     gene            complement(14852..15526)
                     /gene="cmk"
                     /locus_tag="PK513_01800"
     CDS             complement(14852..15526)
                     /gene="cmk"
                     /locus_tag="PK513_01800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOO81217.1"
                     /translation="MSKNIIAIDGPAASGKGTLARSLAARLGFAHMDTGALYRATALE
                     VHDAGLSAQDENDARDGAQILAQKIANAASPSDVLDNPALRTDQIGQQASIVAAHPRV
                     REVLKNIQTDFAENPGKGYNGAILDGRDIGTIIVPDAPLKLFITANVEIRAKRRLKEL
                     QSRGIAATYGAVLKDMRERDARDADRKAAPMKPADDAIIIDSSDLNAGEMLEKAVSLA
                     KERLKL"
     gene            complement(15526..16854)
                     /gene="aroA"
                     /locus_tag="PK513_01805"
     CDS             complement(15526..16854)
                     /gene="aroA"
                     /locus_tag="PK513_01805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOO81218.1"
                     /translation="MNALISHKTKILTGRPRIPGDKSISHRALMFGALTIGETMISGL
                     LEGEDVLCTAQAMRGLGAKIEKSPDGLWRTYGVGIGNLQTPAAPLEMGNSGTSTRLLI
                     GLVGGHPIKATFTGDASLSKRPMRRVITPLEMMGAGIEATDGDKLPLTVTGPEDTLAI
                     EYRLPVASAQVKSAILLAGLNARGTTTVIEEKPTRDHTEKMLTAFGIDVQIETLENGA
                     SAISILGQQELQPGAVDVPADPSSAAFPAVAAVLHEGSEITLANIAISTRRNGLYLTL
                     QDMGADITFENKRLQAGEEVANIIVRGNGALKGIDVDPARVPSMIDEFPILAMAASCA
                     NGTTKMTGLAELRVKESDRLLMVAEGLKTCGVNLEMGEDWLTIHGTGKPPKGGATIQT
                     ALDHRIAMSFLVLGSVTDEPISIDDGTPIATSFPNFVKLMNELGCDIKVL"
     gene            complement(16910..19036)
                     /locus_tag="PK513_01810"
     CDS             complement(16910..19036)
                     /locus_tag="PK513_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO81219.1"
                     /translation="MSPKRHDKPKRLNRALTSSSSQIWKSRLSWRIAAAVFMTILLVQ
                     TMIMNFGTVPAFRQRLLDDLKETNSTAIAAMINPSAGYNEAPFTSEQAARFMIISNVK
                     GISIYSYQNESIASYGAAPSIKKEHMTARGHSKYMPDIGLYEIVLNAADLKRPYYIAI
                     KIDADSVQEQIAYFITDTIKIMLIMSLFVTTVLMIALGHWLLEPMLFMRSNLLAAFKA
                     PEEPDIPKSPFDNRDEIGSAIELTQKLIHQNAENLTRIKSAAEDKIHKLAYYDTLTGL
                     PNRTLFVQTLNEQARQKQDGSAERFAVVTVDLDHFKDINDSMGHNIGDAILRSVARRL
                     RSAMPESAVVSKSGEDEYVIMMPLSTEISSSRAVAEKVWHVIRSEPFKVFNESFQVRA
                     SIGVATFPDDAIDPDQVLKNADIALNRAKEEGRDTIKEYSEDFDRAVQARFQMLRNLR
                     DAMEHDQLELYYQPQIDLRSGEIIGAEALLRWFKPDNSKEGGTFISPADFIPVAEQSG
                     LIVPIGEWVMLTACKMAKSWKDEHGIDIRIAINVSAHQFSQSDICAFTQKTISEVGID
                     AHKVELEVTESVFMDDISHTIQTLQNLHAIGVELAIDDFGTGYSSLSYLRQFPIDRLK
                     IDQSFIRNALNDQDDASIARTIIALGHALNLKVIAEGVETIDHEAFLIKEGCDEVQGF
                     RYSRPVPAKDFLHFAKNYKNDLNPVD"
     gene            complement(19271..20464)
                     /locus_tag="PK513_01815"
     CDS             complement(19271..20464)
                     /locus_tag="PK513_01815"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81220.1"
                     /translation="MSWQSLLNPDIQEFITAHEKDDIAALALKKPPVADWDYKLILDQ
                     IKSRQKARQKIPLWLTHHKNIIFPPPDLLEQASSAATARYKAGLIQGQNFVDLTGCCG
                     IDTCAFTEHFTNGTCIEKDKSAASLLAHNLTLLSSVPVEIINTSAENFIKTMPPLDVA
                     LIDPQRRDSNQKGKYRFEDCAPNILSLLPNLLEKTRTVIVKTSPMLDITQGIATLEST
                     GASAREVHCIQWRSECKEVLFILNNNTKTMPAIIAVSLDDSGNPLYEMRFTQEEEKTC
                     RAPLSQPLSYLYEPGPAFQKSGGFNMIAPKYAVHKLAPQTHLYTSDEWVADFPGRSFE
                     IIGQYPPKAEKIPLKQAHITVRNFPINAPTLRKKLKIKDGGQDYLFACTLANGTKTML
                     HAQKI"
     gene            20489..21583
                     /locus_tag="PK513_01820"
     CDS             20489..21583
                     /locus_tag="PK513_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81221.1"
                     /translation="MKMTFPKWYDLHVHLRQDPLVGPIVQSQIDMGCCGVLAMPNTKP
                     PVAKVFRDDPLDYWCIEDYVDLIKKSGGDAFQDILVPLYLTKDTMPEMIEKGTKADVL
                     RACKYYPPHGTTGADFGYPFMNFFDNGVFEAMQDYGVVLCVHGEEHHMRGEDYFAAGT
                     NAEDFFYRERMPKLVEAFPDLKVACEHVTTATAVAFVQGAGVNVAASITPQHLLYTVG
                     HLLQGLKYHLYCLPLLKFEEDRAALRAAAVDPANAKFYAGTDSAPHTTKATDCGCAAG
                     CFTGAIAPQLYAMAFEEAGVNLETKAGQKVFEAFLCRNGPAFYELAAAKETFTLEKAF
                     QPVEMLETPEGTITPLPLGLKQDGLPWRIS"
     gene            21684..22589
                     /locus_tag="PK513_01825"
     CDS             21684..22589
                     /locus_tag="PK513_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81222.1"
                     /translation="MELIRGSTEHYFMKVLDDLKGQPTHWYGLHFCLSKILDHTDLVS
                     DITMMAQKIDAARARSDVFADELYGVLKDYFKGSLCVFEDLDVFALVQVRDKMDIKHL
                     DEAFNKMKAKLPKGYAGMDGLANQLRNYQKFADEKVLSARKFEAYQAMADKHKISSIQ
                     VRRKRRESPLVMVVEDDRFTAHYASMILSTDFDLIVCKDGEEAVQAYIEHAPDIVFLD
                     IHLPGMSGHDVLQSLHAVDPQGFVVMLSVDTVRDNIVRASQMGARNFVKKPFTKERLI
                     EIVKNSPYVRELTRSDRAGSETMFH"
     gene            complement(22775..23377)
                     /gene="folE"
                     /locus_tag="PK513_01830"
     CDS             complement(22775..23377)
                     /gene="folE"
                     /locus_tag="PK513_01830"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOO81223.1"
                     /translation="MVSSKIVEIGSDKKPSREEAEEAIRILLRYIGEDPAREGLIETP
                     ARVVRAYDEFFEGYNQNPAKELSKTFEDIEGFDDIVLVKDIEFTSHCEHHMVPINGVA
                     HVAYWPDEKVVGISKLARIVDILSSRLISQENMTSGIAECIETTLKPKGVAVYIDADH
                     QCMSIRGVKKRQSSTVTSTFTGKFKEEAVQTRFLRMIDKN"
     gene            23461..>24377
                     /gene="cysS"
                     /locus_tag="PK513_01835"
     CDS             23461..>24377
                     /gene="cysS"
                     /locus_tag="PK513_01835"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO81224.1"
                     /translation="MSSPQFFNSLTRKKEDFVPLNPAHVRLYACGPTVYNYAHIGNAR
                     MAVVFDLLTRVLKSLYPKVTYVSNITDVDDKIIASHKATGEPIDVITRKYEKIYNDDM
                     AALGCAAPDIQPRATEHIGEMIAMIEALIEHGHAYEADGHVLFHVPSFEGYGGLSGRS
                     RDEQIAGARVEVASYKKDPADFVLWKPSTDDEPGWDSPWGRGRPGWHIECSAMSAKHL
                     GLPFDIHGGGADLKFPHHENEIAQSCCASGHEDDLSAFAKVWVHNGFVTVEGEKMSKS
                     LGNFLLAHDLIKDVPGPVLRLALLSAHYR"
BASE COUNT         5176 a         5169 c         7153 g         6879 t
ORIGIN      
        1 ccccaaaagc gcctttttat atccccatgg taatcaggca acctttaccc acacttgtca
       61 gaatacgtta atttagttaa tattttatgt aaaatcttaa gatttatacg ccttttggtc
      121 agtaaaaatg ggctttttaa ggtggaaaat taaccaaatt tttatggttt tgaataggaa
      181 atataaagta accaattgat ttatatggtt tattgataga ttgtattgtt tcttgtcaga
      241 gctttggtaa cctgaccaac tattccacag gaagcatatt gactgcaggc gttaccctcc
      301 attaagaaga ggcgttgaaa tcggtgtgac gccataagaa atgcatgtca caaaaatcaa
      361 taactgactt ttataaatga tgcctgagcc tagtgtagcc cggaaaatgc ttagtaacga
      421 taatcgtctt ttttgaacgg accgttgggt gaaacgccaa tatattcagc ctgtgcatcg
      481 gtgagttttg aaagctgcgc gccgactttt tccaggtgca gggcggcgac tttttcatcc
      541 agatgtttgg gcaggacata gactttattg tcgtaggcgc tgtggttttg ccagagttcg
      601 atctgggcca atgtctggtt ggtgaacgag gcgctcatca cgaaggacgg gtggccggtg
      661 gcgcagccca agtttaccag gcggccttcg gcgagcagta agatacgttt gccgtcgggg
      721 aattcgatct ggtcgacttg cggttttatg ttggtccatt tcatgttgcg cagcggttcg
      781 acctggattt cgttgtcgaa gtggccaatg ttacagacaa tggcgttgtc tttcatttcg
      841 cgcatgtgat caacggtgat gatgtctttg ttgccggtgg tggtacagaa aatgtccgca
      901 cgcggggcgg cgttttccat ggtgacgact tcgtagcctt ccatggcggc ctggagggcg
      961 caaatggggt cgatttcgga gacgagcacg cggcaaccgg ctttccgaag ggattcggca
     1021 gaacctttac cgacgtcgcc gtaaccgcag accatggcga ctttgccggc catcatgaca
     1081 tcggtggcgc ggcggatggc atcaaccagg ctttcgcggc agccatattt attgtcgaat
     1141 ttggatttgg tgaccgagtc attgacgttg atggccgggc agcgcagctt gccttttttc
     1201 tccatctcat acaggcgcag gacgccggtg gtggtttctt cggagatgcc tttgacatca
     1261 tcgagcaatt tcgggaaact ttcatgcatg cgtacggtca aatcgccgcc gtcatcgagg
     1321 atcatgttgg gagtccatat tcctccttgg ccatccgggc cttcgatggt ttggtcgatg
     1381 gcccaccaga attcctcgtc gttcatgcct tttttggcaa agaccggtgt gcctgtggcg
     1441 gcgatagccg cagcggcgtg gtcttgcgtg gagaagatat tgcaggagct ccagcgcact
     1501 gtggcaccca aggcttgcag cgtttcgatg aggacggcgg tttggatggt catgtggagg
     1561 caaccggcaa tgcgcgcatc ttttagaggc tgtttggtac catattcttc gcgcaaggcc
     1621 atgaggccgg gaatttcggc cagctctatt tccttgcggc cccattcggc cagtgaaata
     1681 tcggcaaccc agtagtcatt ttccaagctc ttcggcacag cgctcatttt gtttagatcc
     1741 tctttaaaaa ttaatgattt gggcttacta taggggcatg agcatgaaat tcaatgaaga
     1801 aagttcgaag ccgccattta tatacaaacg caaccctttg gtggagcatg cgtggcgaat
     1861 ctttgccctg aaagagggga aggctgatta tgagcctgtt ggcgaatata ctgtgctgga
     1921 tttagatgaa ccttctgatt taactgagaa aaaaatggct aatcttattg ccattctgaa
     1981 cggtcgtcgt gaacttcaac agttgggcga gcttacaaaa tcccgtttgt tatttacgca
     2041 agtgcctgag gaagaaggtg ctattaataa aaaattaatc tttcgcacct acgatggaga
     2101 tggcatttca aaagagaatg ccattcttgt aatagaaaaa ggtgtattta aagatggcgg
     2161 gcaaacctga attttcaact gtaattcaac agtttttgag tgcagcgagc gggccggttg
     2221 aaacggtttc gccggcgcat aatttggaaa aaccaatggg gctggatttg cggcgttaaa
     2281 acaaccagag caaagggtgt aataaaatgt caggattacc tccccaaaaa gagctttgtt
     2341 gtctttttgc agagaatgca gcgaatatca cgcaagttag tgccagtgca gagtgcactt
     2401 taacgttcga tcctgaaggc gggccaaatt aggtcggtag gtcacggtgt gtaacgtgag
     2461 tggcgaggcc acatttggag agccagcggt ttatttcttc ggtgacaaaa acagaacggt
     2521 gacgtccccc ggtgcagccg atggcgatgg tcaggtaatg tttgccttct tccatataac
     2581 gggggatgag gggctctaac aaagtttgaa accgctctat aaatccggaa aagtcaggat
     2641 ctgttttgat gtaatcgcct acggcggcgt ccagtccggt tttaggtttt aaggttttgt
     2701 tccagtgcgg gtttttcagg aagcgtacgt ccataacaat atcggcttcg cggggtaagc
     2761 catggcggaa gccaaaggaa atcaaagaaa tacttaaggt tttttcgtgg cgggagccaa
     2821 aatggccttc gagaatgtgg cggacgtcgt gcacggagag gtctgagctg tctatggtga
     2881 gatcggcctt gtctttgatc tggcataata aatcacgttc gcgggcgatg ccgatgctga
     2941 ccggggcgtc atcggccaac gggtggcggc ggcgggtttc ggtgaagcgc ttttgcagca
     3001 ctgcgtcatc gcatgtgatg tagatcagga gtgcttttat cttttttacg gtttccagta
     3061 ctttatcggg cgcaaagccg cgtgtgcgtg tgtctatgct tattgctaaa gtgctaggat
     3121 gggggctgtc tttgatgaga gcggggacga gacttagcgg gaagttatcg aagacttcgt
     3181 agcccaaatc ttcaaggttt ttgaggactg aggacaggcc tgcaccagac aggccggtaa
     3241 tgattagcgg tgtttgattg gtttgttcgt tcatatttta ttcaatatca cagtgaaccg
     3301 ggcaccgcgc ggtttttctt cggggccgta gatgttttca gcgaagatct ggccgccgtg
     3361 agcctcaatg atttgacggc aaattgacag gccaaggcca gaatgttggc cgtaatgttc
     3421 gtgctgtggg cgttccgaat agaaacgctc aaagattgtt ttcattttgt tttcagggat
     3481 gcctgggcct tcatcatcga ttgtaatgtg tacttttgtg gccaaggggg ttacgttgac
     3541 ggttattttg ctgtcggcgg ggcagaaaga cagggcattg gagagcaggt tttggaagac
     3601 ttgcgctagt ctgctctcca gtccccagac aaaaatattt tttggctggg tattatgaag
     3661 ttcaatggtg gctttagggg tttggataga gcgtgtccag tcttgttcag cgccgttaga
     3721 acgctcaagc gggttggcag cgtaagtttc aagcagggtt gtcagcaatg tgtgcaagtt
     3781 tattttttca aacgcttcac gggatagttc cgaatcgatg cgtgaggcgt tggaaatatc
     3841 tgaaatcaga cgatcaaggc gctggacatc gtgtttgatg atgtccatga gtttttttcg
     3901 atctgaggtt tttttgacga tggaagctgt ttcgatagcg ctattgagag acgttagtgg
     3961 atttttgagt tcgtgtgcga cgtcggcggc gaagcgttca atggagtcca ttcggttcca
     4021 taaggcgctg gtcatttgac gtagagcgag tgataactcg ccaatttcgt cgtgccggtg
     4081 agacaggtcc ggtatgtcgt cggatcgagc ttgaccgcgg cgtacgtttt cggcggcgtt
     4141 tgcaagtttt ttgagcgggc gggcgatgac tccagagaga tagatggaaa gcaggattgt
     4201 catggccaag gcccctgaaa agatttttaa gacgtcaatc cagacgcggg aaatatcttg
     4261 ttcaatttca gtgctttcat gggttagcaa tatagcgccg cttggttttt cattcattaa
     4321 gagcggggca gccgcagata aaatgatacg cccttgtttg ttttgccatg cggaaagggt
     4381 aatttgttta ttgagtgcgt ctataaggcc tggggggatt tcagtatttt ccgggtaggt
     4441 cggaagagac tggtgttccg gcaggagatc gataataaag cgcatcattt ttttcagggt
     4501 ttttatgcta tgaaaatcct gtgtttttct gtcttctaag gcccaaaggc gatcatcgtg
     4561 gtctggcagg gtgcgggtgt cgagaataag ggttccttga gtgttaaaaa tacgaatgcg
     4621 ctgacggctc gttttggcaa agcgagaggc aagtttctct aaatttttga atatgacacg
     4681 atttgcatca atatctaggt tggtgttggg gatttctttt tctgaaaggc ttgtggatat
     4741 taaattcagc tcggtttcaa aagtttgcag acgcgcattg atgaggttgt tttggtattg
     4801 tccaagatag agcattccaa acatcaggat gagcagggtg atagcattga ctgcgataat
     4861 gcgcagggtg ataccggtga tacggcgctc ggggccgccc cagtaaaaat cgagcaagtg
     4921 gtcaaattcc ggtaaatccg gacgcacggg ggccagacgg aaccagcggg ctttgtcgcc
     4981 gttgtgattt tggttctcag ttttctttgt atcggtatcc gacgccataa agtgtttcaa
     5041 tctggttaaa gtcagggtct gtttttttaa actttttgcg caggcgtttg atgtgggaat
     5101 cgatggtgcg gtcatcgacg tagatgtttt caccgtaggc catgtcgatg agttgatcac
     5161 ggttacggac aattccaggg cgctgggcca aggattttag gagtaagtat tcggtaactg
     5221 tcaggttgac gggttcattg ttccatgtac ataggtggcg ctggtcgtcc atttccagcg
     5281 ggccgtatgt gtatttctca gtggcatctt gcgttttttg cgctttgccg ttttcctgca
     5341 taaggctatg acgccgcagg agcgagcgga tgcgttcgat cagcaggcgc tgggaaaaag
     5401 gtttggagat gtaatcatct gcgcccatgc gcaggccgag gatttcatct atttcatcat
     5461 ccttggatgt gaggaaaatc acgggaatag ttaaagtttc gcggagtttt tccaggactt
     5521 ccaagccgtt catgcgcggc attttgatgt cgagaacaat cagatccggt gtttcttcta
     5581 gaatgccttt aaggcctgct tcgccgtcat tgaaggtggt gatttcgtag ccttcggctt
     5641 ccagtgccat tgacacggag gtgagaatat tgcggtcgtc atcaacgagg gtaatttttg
     5701 cactcatgag gttaagtatg cgctgatttg cctgcatctt tcaagacatg tcctgcaagg
     5761 tataacgatc cggtgatgag gatgaggccg ggtgagaggc tggtttgctc gatatgggtt
     5821 agtgcgtttt ggtagttttc gaaaatttgt ccgccggata gggttttttg taggtcttcc
     5881 gggctttggg ctttgggttc accgggaata gtgattaaag acaatgtatc gacaaagggg
     5941 cgtaggggcg cgtaataggc gcgtgcgtct ttgccctgga gcatggcgag aatgatgtgg
     6001 agaggccggt cgtcttgttt tttccatata gctacttgtt cagcaacggc gtgcccggca
     6061 tcttcgttat ggccgccatc aagatatagc tgccagtctg gtgaaagatt gtaatcttcg
     6121 gtattgagtt tttgcaggcg ggcgggccat tgggccatgg ccaatccggt tttaatctgt
     6181 tcgtctgtga gcgggaagcg atctttgatg agttccaatg ttttgagggc ggcggctata
     6241 ttggcgcgtt ggtgcgggcc gatgagggcg caggttagat ttttcggaac gtcttcggtt
     6301 tgataaagcg cggcctttat gcgccgggca gtttcttcga acacgtctat aacgcctgcg
     6361 tttaaggcct ggttgctttg gggtgcgatg atgcatggca ccttcggttt cataatgcct
     6421 gctttttcgg cggctatggc ggtatatgtg ttgcccagat gttcggtatg atcaagtccg
     6481 atggaggtta tgattgtgac gagcgggttt tcaatgacgt tggtgcaatc gaggcgacca
     6541 cccatgccga cttcgagcag gcaaatatca gcgggcgtgc gggcgaaggc ggcaaaggcg
     6601 atagcggtgg tgatttcgaa aaaggttaag tcgccgcctt catttaattc cagtgtttcg
     6661 tcaatcaggg tttcgagggc ttgatcatcg atttcctggt tagccagtat gatgcgttca
     6721 ttgaaatgaa tgagatgcgg tgaggtgtac gtgtgcactt tatatccggc gctttccaaa
     6781 atggcgcgaa gcatggcgat ggtggagcct ttgccgttgg tgccggcgac gtggatgacg
     6841 ggggggaggt tgcgttcagg atggccgagc ttttcgagca ggtccagata gggcgggcgg
     6901 aaagacaagt cgatcgtttt attgcgattt aagcgataaa gctggtgcag cttgtcttcc
     6961 agagattttt ccgggtgatt gaggtcactt tgatacatag tgccagcttt atgttatgcg
     7021 gctttttttt cagtattcat taataaccta aggatacggc ccagtgtttc gtgcaggtct
     7081 ttacggttga cgaccatatc gaccatgccg tgttcctgca gatattcggc ggtctggaaa
     7141 tcgtcaggca gtgtttcgcg tacagtttct tcaatgacgc ggcgaccggc aaagccgatc
     7201 attgagccgg gctcggccag atgaatgtca ccaaccatag cgaaggaggc gctgacgccg
     7261 ccggttgtcg ggtctgtgag caggacgata taaggcagcc tggcctcttt gaccatttcc
     7321 acggcgatga tcgagcgtgg catttgcatg agggagagca tgccttcttg catgcgtgcg
     7381 ccgccggatg aagggatggc tatgaaagcg gcatggtctt tcacggcttc ttgtgcggct
     7441 ttgattaatc cttcgcctac ggctgtgccc atagagccgc ccataaagct gaagttaaaa
     7501 gcggctacga cagctttttg tccgtcaatg gtgcctttgg cgatggtgat ggcgtctttg
     7561 aggccggttt tactttgggt ttctttcagg cggtcagcgt attttttgcg gtctttgaat
     7621 ttgagtgggt cgtgcgggac ggacggcacc ccgagggttt cgtatttacc ggcatcgaac
     7681 attattttca ggcgttcttt ggcggtgatg cgcaaatggt ggttgcagtg atagcaaacg
     7741 ttcatatttt ctgcgaggtc gcggtggaac agcatgcctt cgcacgatgg gcattgttgc
     7801 cagaggttat cgggcacttc cttttgttcg cctaccaaag aacggacgcg gggtctgata
     7861 aagtctgtta accagttcat gtaaaatacc ttttattgtc gtaaaatacc gccactttat
     7921 cggcttttgg taggcaaaag tcaagctaaa gtggaattta tgacagggca tcggacaggg
     7981 ctttgacttg ctcggaaacg gcgctggcgt cgcgtttttc tgcgatggtc tggacgatgg
     8041 atgagccaac gacgatggca tcgcccaggg cggccatttg cgccgcatct tgcggggttt
     8101 tgatgccgaa accgatggcg ataggcagat cggtgtgggt tttgatctgt tcgatatggg
     8161 gttttatttt gtttaaatcg gcctgtgcgg cgccagtgac gcctgtgatg gaaacgtaat
     8221 agagaaagcc gctggcgcct tcgaggattt tgggcaatct gcccatggtt gttgttgggg
     8281 tgagcaggcg gatgatgtcc agattgttgg ttttggcgat ttcctgcgct ttgcccgctt
     8341 cttcgggcgg cagatcgacg atgatgaggc cgtctactcc ggcggcgctg gcatcgttca
     8401 taaatttttc aacgccgtat tgcaggatgg ggttgaaata acccatcagg acgatggggg
     8461 ttgtattgtt gcctttgcgg aaatcgcgaa ccatttgcaa cgtttgtttc atgtttgcgc
     8521 cacttttcag ggcgcgctgt ccggcaagct ggatggtggg gccgtcggcc atgggatcgg
     8581 tgaagggcat gccgagttcg ataatgtcag cgccagcttc cggcagggcc ttgagcacgt
     8641 ccaaggaggt tttggcgtcc ggatctccgg ccatgataaa ggtgatcagg gcgggacggt
     8701 tgagggcttt aaatgtggct tctatacggc tcatgatttt actccaacat gcaccaaaac
     8761 ttcgttgacg ccttgttcga aaaagcgcgg gccgtaaact tcaaaatcgg tggtgtatga
     8821 gcgctcgata tctgaagacc agatggcttc ccaggtttcg atcagggctt tgggttgttc
     8881 accggccgcg ctcatcacgg cgtattcctg cggctgtacg cgtacgcggt gcatatcaat
     8941 agcgcacgcg tcattgctga cgcggtagcc gatagtgagg cggtagggcg cttcgtgatc
     9001 gccttgataa tcgctgtaga cggcatagat cgcgtcgtca gttttgtcga gaatttgttg
     9061 tccaatgctt tcggaaaaaa atcgttccca cagggcgttg atatcgtcag tacctttttg
     9121 attgtcggtt atggtggaaa tgccgacaac atcaaaagct tcaacttctg ctagcccggc
     9181 gcggacttcg taattagcca tggatcttca ctcccagctt gtcggcgatg gtgaagatgt
     9241 ctttatcgcc gcggccgcag agattcatca cgatgatatc gtctttgggc agggtggggg
     9301 cgcgtttcat gacatgtgcg aaggcgtggg cgggttcgag cgcgggcagg atgccttcga
     9361 gccgggccat ggtttgaaag gcttcaatgg cttcatcatc agtgacggag acgtattcca
     9421 cgcgccgttg atcaaacagc caggcgtgtt cagggccgat gccgggataa tccagtccgg
     9481 cggaaatcga atgggcttct tgaatctgac catcgtcatt ttgcaggaaa taggaattca
     9541 tgccgtgcag cacaccgggc ttgccgccgg tgaggctggc ggcatgctgg cctgttccaa
     9601 cgccatggcc gccggcttcg acgccgatca tcttcacatc tgcatcatcc aggaacgggt
     9661 ggaacaggcc catggcgttg gaacccccgc cgatacaggc gacgagatgg tcaggctggc
     9721 ggccttcttt ttccatgatt tgctttcgca cttctgtacc gatgatggac tggaaatcgc
     9781 ggaccatggc cgggaagggg tgcgggccgg caacggtgcc gatcaggtag tatgtgtcat
     9841 ggacgttggt gacccagtcg cgcatggctt cgttcatggc gtctttgagc gttcctgcgc
     9901 cgcttttgac ggcgcggact tcggcgccaa gcaacttcat gcggaaaaca ttgggagctt
     9961 ggcgttcaat atcggttgca cccataaaca ctacgcatgg caggttaaag agagcgcaga
    10021 cggtggcggt ggcgacgccg tgctggcctg cgccggtttc gcagatgatg cgggttttgc
    10081 ccatacgctt tgctacgagg atttgaccga ggcagtgatt gattttgtgc gcgccggtat
    10141 ggttcagttc atcgcgcttg aaatagattt ttgcgccgcc gagttcttcg gttgtgctcc
    10201 cagcaaaata gagcggggag ggacggccaa catagttgcg ctggaggttt tccaattctt
    10261 cccagaattt cggatcggct ttggcggctt cataggcctt ttcaacctcg agaatgaggg
    10321 gcatcagggt ttcggcgacg tagcgtccgc cataggggcc gaaatggccg cgttcatcgg
    10381 gttggttacg gtaagaattg gtttttaggt ttttttggct catgggcgct tatatagcgc
    10441 ttttgactgc gtcgataaag gctttaattt tagccgggtc cttaacgccg ggggcgcttt
    10501 cgacgccgga ggagacgtca acggctgttg gggagagttg ggacagagct tcagctacat
    10561 tttcgggtgt gaggccgccg gagagcatcc agggccgcga gaaggttttt ccggcgagca
    10621 gcgaccagtc gaagctatgg ccggtgccgc cgggcagggt tgcggcctcg gggcggctat
    10681 cgaagaggag ccagtcggcg gtttcttcgt aagcggggac ggtttcgagg tcggcgggtt
    10741 cgcggatttt ggttgctttc atcaccggca tggcgaattt gtccttaatt tgctgcacgc
    10801 gctcggggct ttcactgccg tgaagctgca gcatatcgag cgggacaatg cccaagatat
    10861 gttcgagttg ttcgttggtg gggttaacga acaggccgat ggccttgacg ccggtgggaa
    10921 gcgtcaggct gagttccttt gcactgtcgg cggcgatgtt acgcgggctt ggcggataga
    10981 agacaaagcc gacatagcgc gcaccggcgg acgtggcggc gctgaggttt tccgggtctt
    11041 tgaggccaca gattttaacg gcagtcattt gagactatcc atgatctctt gtgcggcttt
    11101 ggccgggtca ggggcgcctg ttatggggcg gccgatgacc agatgggtgg ctcctttttg
    11161 caccgcttca tttggggtca tgacccggtt ttggtctgct tttgcgcttc cagcggggcg
    11221 gatgcccggg accatggttt caaatgaatc ggcgcatgat tcgcggatga gggtgatttc
    11281 atgggaggag cagacgacgc catcgaggcc gctggtttcg gcgagccggg ccatttggat
    11341 aacgcgttca gggaggcctt ctttatatcc tattttacat aattcatttt ctgagaggga
    11401 ggtgaggatg gtgacggcca agagttttgt ttgcggcgtg caggcctctt ttgccgcttc
    11461 catcatttta cggccaccgg cggcgtggac gtttaggtag gctggggcga aattggcgca
    11521 gatggtgcgc actgcgccgg cgaccgtatt ggggatatca tggaatttga ggtcgatgaa
    11581 cagggcggcg tcgggagcgg cgttcttgac ggtttctatg cctgcagggc cgtgggcgtt
    11641 gatgaattcc ataccgagtt tcagtgcgcc ggtgacggga ccgattgtgc gtgcccaaag
    11701 ccgggcttgt tcaaggtcgg cggtgtcgag ggcacaaaat atcaaaggac gagaactcat
    11761 ccttgtttta tggcggaatt ttgggcttta ggcaaggtgg tgtttttctg ctgcgctggt
    11821 gggtagtgcg cggaaaagtt cggatggcgg agcatttgcg ggcgatgttt ttttattttg
    11881 cgcgtttgcg agttcttttt ccaaagcttt gatggttttg cgttgttggc ggctgctacg
    11941 gcgggtgggg gcggtgttca tccacatgat gaatgcgccg aagataaatc caagcgccat
    12001 gaacaacaat gtgagcaggt agagtggaat ttcgagcgga tcgtggacag ggctatagtt
    12061 cagcgcgata ttctgacggt ttgatacagc gaaagccacg agggcaatgg caatgataaa
    12121 tccaaaaatg gcgcggatga agctcatggt ttaagcttta gtttctgaat ttagtaccag
    12181 gaatcctcgc tggacgagga atcatcactc ggtgttttgt cattaagacg ttcgcgcagg
    12241 cccttgccag ttttgaaaaa tggcaggcgt ttggcttcga catgaactga ttcgccagtg
    12301 cgcgggttgc ggcctatacg cgcatcgcgg tgtttgacag aaaaagctcc aaatccgcgg
    12361 agttcgacgc gatttccatc gaccagagcc tgtgtgattt cgtcaaaaat tgtatcgacg
    12421 attttttcga tgtcgcgcaa atacaagtgc gggctgcgtt cagccaaacg ttgaataagt
    12481 tctgattttg tcattacact tttaatcctt ctttatagag gcttagctcg tatctcacag
    12541 gctgtctttt atatacggat gagtctacac tgcctgaaat ctaacctcgg cgtcaagaaa
    12601 aagcacaaaa aattcggcat taataacttc gtatagataa tcaatttagt tatccgtgaa
    12661 gaatatggtg agcgtggtct ggaccatgtg tatgtctttg gaattgaaat agcagaatgt
    12721 tttgcaatga aatcgtgcaa gagcatgcct gatacggctg tttggccctt cgattgcggt
    12781 cgtttcagac tttgtttcca catgcatgtg ttcgatgaag tgtttcaagt ataaaaaagc
    12841 cgggctttgt cggcccggct tttgaatttg tatatgagac gtgttttagt cttctttttt
    12901 cttatcggct tttttgtcag cttttttatc tgcgcttttt gctttggcct catcatcttt
    12961 tttcttggat gttgtttttt tggccgcagc ttttttcgcc ggggtctttt tgggcttttc
    13021 ttctttggct tctttgcctt ttttctcttc taaagcggcg ccgaggatgt cacccaaaga
    13081 tgcacccgat tcggttgagc cgaactcctt catggcttgt ttgtcttcat cgacctcgcg
    13141 ggctttgatt gacagggcca ctttgcggga ttttttatcc atagaggtga ttttcgcatc
    13201 gactttttcg ccaacagcaa agcggtccgg gcgttgctca gagcgttcgc gcgacaggtc
    13261 agcttttttg atgaagcctt tgatgttgtc gttgaccgag acttcgatgc cgccggaggt
    13321 gatttcggtg acagtgcagg tgacaacagc gcctttcttg acaccgtcca tcgcgccttc
    13381 gaatggatct tctgtaagct gtttaatgcc tagagcgacg cgttctttgt ccggatcaat
    13441 ctcaaggatt ttgaccttga ttttgtcgcc ggttttatag gctttgaggg cttcttcgct
    13501 ttcgtcttcc caggaaatat cagacaagtg aaccatgccg tcgatttcat catcgagagc
    13561 gacgaacagg ccgaattcag tgacgttgcg tacttcgcct tcgaactctt cgccttcttt
    13621 atggctggct gcgtaagcgc cccagggatt atcctggcat tgtttcaggc ccagtgaaat
    13681 tcggcgtttt tcttcgtcga cttccagaat cataacctca acttcttcag acgttgaaac
    13741 aattttgccc gggtggacgt ttttcttggt ccatgacatt tcagagacgt gaaccaggcc
    13801 ttcgatacca tcttccagtt caacaaaagc gccataatca gcaatgttgg tgatgcggcc
    13861 tttgagccgt gtgccgattg ggaagcgcgt gtcgatgtct ttccacggat cgtcttcgag
    13921 ttgtttcatg cctagcgaga tgcgctggtt ttcttcgtta aaacgaataa cctgtacgtc
    13981 gattgtctga ccgacctgca gggattcaga tgggtgattg acgcgtttcc atgagatgtc
    14041 ggtaacatgg agcaggccat caacaccgcc aaggtcaacg aacgcaccgt aatcggtgat
    14101 gtttttgaca acgccttgca ggacttgacc ttctttgagg ttgtccatca ggtctgaacg
    14161 ggcttcggcg cggctttctt ctagaatggc ccggcgtgaa acgacgatat tgccgcgggc
    14221 gcggtccatt ttcaaaataa ggaacggctg cggtgtatcc atcagtggcg atacatcgcg
    14281 tacagggcga atgtcaacct gagagccggg aaggaaggct acagcgccgc cgaggtcgac
    14341 tgtgaagcca cctttgacgc ggccaaagat tacgccggtt acacgctctt ctttttctga
    14401 tgctttttcc agttcgatcc aggcttcttc gcggcgggct ttttcgcgag atagtacgga
    14461 ttcgccatcg cggttttcca tgcgctctac gaaaacttcg acaacgtcgc cggttttgat
    14521 ttcggcgtct tcgcctgggc gagagaattc ctgcaaggga atgcggcctt cggatttcag
    14581 gccgacatca acgattgcga aatcgtcggt gacggcaata atggttccgt ttacaacgga
    14641 acctgtaaag ttttcctgtc ctttgagaga ttcttcgaga agggcggcaa actcttttga
    14701 gtctgtttta tcttggattt ttgcggctac actggccata tttaaatttc cttcaattag
    14761 ggttagggtt tttttgtgct ttccaccgtt cctgttatcc tttgtttaaa aggagcggcg
    14821 agcacgtttt gaaagcgatg tgtgaatgcg tttagagctt taaacgctct ttggccagag
    14881 acacagcttt ttcaagcatt tcaccggcat taagatcgga agaatctata atgattgcat
    14941 catctgccgg tttcatcgga gcggccttac ggtcagcgtc acgcgcgtct ctttcacgca
    15001 tatccttcaa aacggcccca tatgtagcgg caatcccgcg cgattgcaac tcttttaagc
    15061 gtctttttgc gcggatctcg acattggcgg tgatgaagag tttgagcggg gcatctggaa
    15121 cgatgattgt gccgatatcg cggccgtcta aaatagcgcc gttatagcct ttgccggggt
    15181 tttcagcaaa gtcggtctga atatttttga ggacttcacg tacgcgcggg tgggctgcaa
    15241 cgatggaggc ttgctggccg atttgatctg tgcgcagggc tggattatcg agcacatcgg
    15301 atgggctagc ggcattggca atcttttgag caaggatttg cgcgccgtcg cgggcatcgt
    15361 tttcgtcttg cgcactgagg cctgcgtcgt gaacttctaa cgcggtagcg cggtagagtg
    15421 cgccggtatc catatgggca aagccgagac gggcggcaag gctgcgggcc aatgtgcctt
    15481 ttccactggc tgcagggccg tcgatggcga tgatgttttt actcatcaca ggaccttaat
    15541 atcgcagccg agttcgttca tgagtttgac gaagttcggg aagctggtcg ctatgggggt
    15601 gccgtcgtcg atggagatgg gctcatcggt gacgctgccc agaacgagga aactcatcgc
    15661 gatgcggtga tcaagggcgg tttgaatggt tgccccgcct ttgggcggtt tgccggttcc
    15721 atgaatagtg agccagtctt cacccatttc aaggtttaca ccgcaggttt ttaaaccctc
    15781 ggcgaccatg agcaggcgat cgctttcctt gacgcgcagc tcggccagcc cggtcatttt
    15841 cgttgtgccg ttggcgcagc tggctgccat agcgagaata gggaattcgt caatcattga
    15901 cgggacgcgg gccggatcaa catcgatgcc tttgagtgcg ccattgccgc gcacgatgat
    15961 attggcaact tcttcaccgg cctgaagacg tttgttttcg aatgtaatat cggcgcccat
    16021 gtcctggagc gtgagataca ggccgttacg acgggtgctg atcgcgatgt tggccagcgt
    16081 gatttcagag ccttcgtgta gtacggcggc aacggccgga aaagcggcgg aggacgggtc
    16141 tgcgggtacg tcaactgcgc cgggctggag ttcttgctgg ccgagaatgc taatggcgct
    16201 ggcgccgttt tccaatgttt cgatttgtac gtcgatgccg aaagctgtga gcattttttc
    16261 ggtgtgatcg cgggtaggtt tttcttcgat gaccgtggtg gtaccacgcg cattcagtcc
    16321 tgcgagcaag atggcggatt tgacttgcgc gctggctacg ggcaagcggt attcgatggc
    16381 gagcgtatct tccggcccgg tgacggtgag aggaagttta tcgccatcgg tggcttctat
    16441 gcccgcgccc atcatttcga gtggggtgat gacgcgccgc atcgggcgtt ttgacagtga
    16501 ggcgtcgccg gtaaaagtgg ctttgatcgg atgaccgccg acaaggccga tgagcaggcg
    16561 cgtggaggtg cctgaattgc ccatttcgag cggggcagcc ggcgtttgta aattgccgat
    16621 gcctacgcca taggtgcgcc acagaccgtc ggggcttttt tcaatttttg cgccgagccc
    16681 gcgcatggct tgggcggtgc acagaacgtc ttccccttca agcaggccgg aaatcatggt
    16741 ttcgccgatg gtcagtgcgc caaacatcag ggcgcggtgc gatattgatt tgtcgcccgg
    16801 aatgcgggga cgacctgtca ggatttttgt tttgtgggag atcagtgcgt tcatgcgctt
    16861 acagatacag gttcagggcg cttgattcaa ccgtatttct tgacgggctc taatcgacag
    16921 ggttgagatc gtttttgtag ttttttgcaa agtgtaagaa gtcttttgcc ggaacagggc
    16981 gagaatagcg gaagccctgg acttcgtcac agccttcctt gataaggaag gcttcgtgat
    17041 ctatcgtttc tacgccttcg gcgataactt tcaggtttag tgcgtggccc agggcaatga
    17101 tggtgcgtgc gatggaagca tcatcctggt cattcagggc attgcggata aagctttggt
    17161 cgattttgag gcggtcgatc gggaactgac gcagatatga cagggaagaa taaccggtgc
    17221 caaaatcatc aatggcaagc tctacgccaa tggcatgaag attttgcagc gtttggattg
    17281 tatgagaaat atcgtccata aagacgcttt ctgtgacttc cagttcaact ttgtgggcat
    17341 cgatgcccac ttcagagatt gttttttgtg tgaaggcgca gatgtcgctt tgtgagaatt
    17401 ggtgcgctga aacgttgatg gcaatgcgga tgtcgatacc atgttcgtct ttccagcttt
    17461 ttgccatttt gcaagctgtg agcatgaccc attcgccgat cgggacgatg aggcctgatt
    17521 gttcggccac ggggatgaag tctgcggggg aaataaaagt tccgccttct ttagaattgt
    17581 cgggtttgaa ccaacgcagc agtgcttcgg cgccgatgat ttcacctgag cgcaggtcaa
    17641 tctggggttg gtaatagagt tcgagttgat catgttccat ggcatcgcgc agattgcgca
    17701 gcatttggaa acgggcctgc acggcgcggt cgaaatcttc ggaatattcc ttgatagtgt
    17761 cgcggccttc ttctttggca cggttgagtg cgatgtcggc atttttcaag acttgatctg
    17821 gatcaatggc atcatccggg aatgttgcaa cgccaataga agcgcggacc tggaaggatt
    17881 cgttgaaaac cttgaaaggc tcggagcgga tgacgtgcca gactttttca gcgacagcac
    17941 gtgatgagct gatttcagtt gagagcggca tcattattac atattcgtcc tcgccggatt
    18001 ttgaaactac agcgctttcg ggcattgcag agcgcaggcg gcgggcaacg ctgcgtagaa
    18061 ttgcatcgcc aatgttgtga cccatggaat cgttaatatc tttaaaatgg tccaaatcaa
    18121 ctgtgacaac ggcaaagcgt tcggcactgc catcttgttt ttggcgggcc tgttcgttga
    18181 gtgtttggac aaagagagtt cggtttggca gacctgtaag cgtgtcgtaa tatgccagtt
    18241 tgtgaatttt atcctcggcg gcgcttttga tgcgcgtgag attttcggca ttttgatgga
    18301 tgagtttttg tgtgagctca atggcgctgc cgatttcatc acggttatca aacggggatt
    18361 tcggaatatc tggctcttcc ggtgctttaa aggcggcgag caagttgctg cgcatgaaga
    18421 gcataggttc cagcaaccag tgtcccagag caatcatcaa tacggttgtg acgaagagcg
    18481 acatgatcag catgatttta atggtatcgg tgatgaagta ggcgatttgc tcctgtacag
    18541 aatcggcatc tatttttatg gcgatatagt agggtcgttt gaggtctgcc gcattgagaa
    18601 cgatctcata caaccctatg tcaggcatgt atttagaatg cccgcgcgct gtcatgtgtt
    18661 cttttttgat gcttggtgca gctccatagg aggcaataga ctcattttgg tatgaatata
    18721 tgcttatgcc tttgacgttg ctgataatca taaagcgtgc agcttgttcg ctggtgaaag
    18781 gtgcttcgtt gtaccctgct gacgggttaa tcattgcggc gattgcagtg ctgtttgttt
    18841 ctttgaggtc gtcaagcagg cgttgccgga aggccggcac agtcccgaaa ttcatgatca
    18901 tggtttgtac cagcagaatg gtcatgaaca cggctgcggc gatacgccag cttagcctgc
    18961 ttttccagat ttggctggaa gaagatgtca gggcgcggtt tagtcttttg ggcttatcgt
    19021 gtcgttttgg gctcaacttt ttgttactcg tggttttgtt tgaggttcta aggaaacttg
    19081 tttttgtgtt tgtaggtaac atccagacct aaacattcta cattggcttt aacgcaggaa
    19141 aaagcgaatt tatctattat tttgttaata tggaattcta cgattcttta gctgtctata
    19201 attctaagag gaaaaactta aaaacgtctt tatttttgtg attttagtct aattgagaat
    19261 tgtattttcc ttaaattttt tgggcatgta acatagtttt tgtgccgttc gcaagcgtac
    19321 aggcaaaaag ataatcttgt cctccatctt tgattttgag ttttttacgc agggttgggg
    19381 cgtttatcgg gaaattgcgg acggttatat gcgcttgttt taatggtatt ttttcagctt
    19441 ttggcggata ctggccgatg atttcgaaac tgcggccagg gaaatccgca acccattcat
    19501 cggaggtgta gagatgagtt tggggcgcga gtttatgaac ggcgtatttt ggggcgatca
    19561 tgttaaatcc gccgcttttt tgaaaggcgg gtccgggttc gtaaagatag gataagggtt
    19621 gtgaaagtgg ggcccggcag gttttttctt cttcttgcgt aaagcgcatt tcatataaag
    19681 gatttccact gtcatcaagg cttacggcga tgattgccgg catggttttt gtattgttgt
    19741 ttaggataaa caacacttct ttgcattcgc tgcgccattg gatgcaatgg acttcgcgag
    19801 cagatgcgcc ggtagattca agggtggcga tgccttgtgt gatgtcgagc atgggtgaag
    19861 ttttgacgat tacggttcgg gttttttcca gcagattggg gagcagcgac aaaatgttgg
    19921 gtgcacagtc ttcaaaccgg tacttacctt tttgattgga atcacggcgc tgggggtcaa
    19981 tgagcgcgac atcaagcggc ggcatggttt tgataaagtt ttcggcgctg gtattgataa
    20041 tttcgaccgg gacgctgctg agtagggtta aattgtgtgc gagcagactt gcggcgctct
    20101 tgtctttttc gatgcaagtg ccgtttgtga aatgctcggt gaacgcgcag gtgtcgatgc
    20161 cgcagcagcc agtgagatca acaaaatttt gtccctggat cagtccggct ttatagcgcg
    20221 ccgttgcggc agaagaggct tgttcgagta aatccggcgg cgggaagatg atgttcttat
    20281 ggtgagtaag ccagagcggg attttttgcc gggctttttg gcgggatttg atttggtcga
    20341 gaatcagctt gtagtcccaa tcggcaacgg gaggtttttt tagcgcgagc gcggcgatat
    20401 cgtctttttc atgcgcggtg atgaattctt gaatatcggg gtttaaaagg ctttgccaag
    20461 acatggttgt tagggtaaat cattagtcat gaaaatgaca ttcccgaaat ggtatgattt
    20521 gcacgtacat ttgcgtcagg atccgttagt tgggccgatt gttcaatctc agattgatat
    20581 gggctgttgc ggcgttttgg cgatgcctaa taccaaaccg ccggtggcca aggtttttcg
    20641 tgatgatccg ctggattact ggtgcattga agattatgtc gatttgatta aaaaatccgg
    20701 aggggatgca tttcaggata tactcgtgcc gctgtatctg accaaagata cgatgccgga
    20761 aatgattgag aaaggcacga aggcggatgt gcttagggcg tgtaaatatt atccgccgca
    20821 tgggacgacg ggtgcggatt ttggttatcc gtttatgaat ttctttgaca atggcgtgtt
    20881 tgaagcgatg caagactatg gcgttgttct gtgcgttcat ggtgaagagc atcacatgag
    20941 gggcgaggat tattttgctg cggggacgaa tgcggaggat ttcttttatc gtgagcggat
    21001 gcccaagctt gtagaggctt tcccagactt aaaagttgcg tgtgagcacg tgacgacggc
    21061 cacagctgtg gcttttgtac aaggtgcggg ggtgaatgtt gcagcctcaa ttacgccgca
    21121 gcatttgctt tatacggtcg ggcatttgct gcaggggctt aaatatcatc tatattgttt
    21181 gccgttgctt aaatttgaag aagaccgcgc tgcgcttcgg gcggcggcgg tcgatccggc
    21241 taatgctaaa ttttatgccg gaacggatag cgcgccgcat acgaccaagg ctacggattg
    21301 tggctgcgcg gcgggatgtt ttaccggggc catcgcgccg cagctttatg caatggcgtt
    21361 tgaagaggcg ggggtgaatt tggaaacaaa ggccgggcaa aaggtgtttg aagcgttttt
    21421 atgtcgtaat ggccctgcgt tttacgagct tgcggctgcg aaagagacat tcactcttga
    21481 aaaagcgttt cagccggtgg aaatgctcga aacacccgag gggacgatta cgccgttgcc
    21541 gctgggtttg aaacaagatg gtttgccctg gcggatatcg taactccgta atatatatac
    21601 agaaaattaa ggctttttcc ttaaaattga tggtgggagt ataaaaaacg tccgcaattg
    21661 ggcgtgggca agggtgaagg gcgatggaac tgatacgggg tagtactgaa cattatttca
    21721 tgaaagtact ggatgatttg aaagggcagc caactcattg gtatggtttg catttttgtt
    21781 tgtccaaaat attggatcat acggatcttg tttctgatat tactatgatg gcacaaaaaa
    21841 ttgatgctgc ccgggcgcgt agtgacgtat ttgccgatga gttgtatggc gttttgaagg
    21901 attatttcaa gggttcactt tgcgtttttg aagatttgga tgtttttgcc ctggtgcagg
    21961 tgcgcgataa gatggatata aaacatcttg atgaagcttt taataagatg aaagcaaagt
    22021 tacctaaagg ctatgcgggt atggacgggt tggccaatca gctgcgcaat tatcaaaagt
    22081 ttgccgatga aaaagttttg agcgcgcgta agtttgaggc ttatcaggct atggccgaca
    22141 agcacaaaat ttccagcatt caagtgcggc gtaagcgccg ggaatcgccg cttgttatgg
    22201 ttgtagaaga tgaccgtttt acagcgcatt acgcttcaat gattttgagc acggattttg
    22261 atctgattgt ttgtaaagac ggtgaagagg ctgtgcaggc ttatattgaa catgcaccgg
    22321 atattgtgtt tttggacatt cacttgcccg gtatgagcgg gcatgatgtg ttgcaatcac
    22381 ttcatgcagt tgatccacaa gggtttgtgg tgatgttgag cgtggataca gtgcgggata
    22441 atattgtgcg ggccagccag atgggggcac gtaattttgt caagaagccg tttaccaaag
    22501 agcgcttgat tgaaattgtt aaaaactcac cttacgtgcg tgagttgacg cgcagtgacc
    22561 gggcgggcag tgaaacgatg ttccattaaa acgatggcag ttttaatctc tctcaggttt
    22621 aattccttgc cgcttgcggc gtaatcgtca tcgcgagcgc agcaaagcga tccagcgcca
    22681 caagaataaa ttctggattg cttcaccgcc tttggcggtt cgcaatgacg tgatacctcg
    22741 cctcttgggg tgggggtgtt catttcttcc gaggttagtt tttgtctatc atgcgcagga
    22801 aacgagtctg aacggcttct tctttaaatt ttccagtgaa tgttgatgtt acggttgagg
    22861 attggcgttt tttgacgccg cggatcgaca tgcactggtg gtcagcatcg atgtagacgg
    22921 cgacgccttt tggtttgagg gtggtttcga tacattctgc gattccgctg gtcatgtttt
    22981 cctgggagat aaggcggctg gagagtatat cgacgatgcg cgcgagtttg gagatgccga
    23041 caactttttc atccggccag taggcgacgt gtgccacgcc gttaatcggc accatgtggt
    23101 gttcacagtg tgaggtgaat tctatatctt tgaccagaac tatgtcgtca aagccttcaa
    23161 tatcctcaaa ggttttggag agttctttgg ccgggttctg gttgtagcct tcgaaaaatt
    23221 cgtcataggc gcgtacaaca cgcgctggtg tttcgattag tccttcgcgc gcagggtctt
    23281 cgccgatata gcgcaggagt attctgatcg cttcttctgc ttcttcgcgg cttggttttt
    23341 tgtcgctgcc tatttcgaca attttggatg aaaccatttg aaaaatcctt atgtttgttg
    23401 tcttctagcg tcttagcgct ggattttcca gtaaaaaaca cacaaaaagt agaattagct
    23461 atgtcgagcc ctcagttttt caacagtctg acccgtaaaa aagaggattt tgtgccttta
    23521 aatcctgcgc atgtacggct gtacgcgtgt ggaccaacgg tttataatta tgcgcatatt
    23581 ggtaatgcgc gcatggctgt ggtgtttgat ttgctgaccc gtgtgcttaa gagcctttat
    23641 ccgaaggtta cctatgtatc caacattacg gatgtagatg acaagattat tgcgtcgcac
    23701 aaggctacgg gtgagccgat tgatgtgatt acacgcaaat atgagaaaat ttataatgat
    23761 gatatggccg cgctggggtg cgctgcgccc gatattcagc cgcgggcgac tgagcatatt
    23821 ggcgagatga ttgcaatgat tgaagccttg attgagcacg ggcatgctta tgaggcagat
    23881 gggcatgtct tgtttcatgt accgtctttt gagggttatg gcgggctttc agggcgaagc
    23941 cgggatgagc agattgccgg ggcgcgggtt gaggttgcgt cctataagaa agatccggcg
    24001 gattttgtgt tgtggaaacc ttcaacggat gatgagccgg gttgggatag cccatggggg
    24061 cggggccgtc cgggctggca tattgaatgc agtgcgatga gcgcgaagca tttgggcctg
    24121 ccgtttgata ttcacggcgg tggggcggat ttgaaattcc cgcatcacga aaacgagatt
    24181 gcgcaaagtt gctgcgcatc agggcatgaa gatgacttaa gcgcttttgc gaaagtctgg
    24241 gtgcataacg gttttgtcac cgttgagggt gagaagatgt cgaagtcttt gggcaatttc
    24301 ttgctggcac atgatttgat taaagatgtg ccggggccgg ttttgcgatt ggcgttgctt
    24361 tcggcgcatt atcgcca
//
LOCUS       DAOSWR010000012        23391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1394_length_23391_cov_5.500643, whole genome shotgun sequence.
ACCESSION   DAOSWR010000012 DAOSWR010000000
VERSION     DAOSWR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 23391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23391
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_23391_cov_5.500643"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1098)
                     /locus_tag="PK513_01840"
     CDS             complement(7..1098)
                     /locus_tag="PK513_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81225.1"
                     /translation="MMLVIANVVPVSAGAAQSGAPSLKTSESSSRFFDTQARAVDGGT
                     LMAGKTPIKLWGIQPIEGMPAQFSVNVRAALDAVLGYNKVRCEMKSRDASGVVAQCTN
                     SFDQDLGLYMLQRGFAVVDRAAVFGGAFEESYIQAEKDAQKQGLGVWLQEGSRSSEND
                     GAFMMIAGGILILCLLGAFSVLTLIIMKGFQKVADVQTQSMNMMARERALRDKEREIF
                     ATMLDSEIKANKSKVEAYLVVYDEMLKDLKDPDKKPKYKKAGDIVQAQPAMERSVFDR
                     NTDKLDILGDQLSSEVIHFYARIKSKPDYINLEPEMPVEEAREIVETAYNRAQRMDEI
                     SDRLIDLFERGGYARGGPKNSDSELSYDL"
     gene            1305..1829
                     /locus_tag="PK513_01845"
     CDS             1305..1829
                     /locus_tag="PK513_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81226.1"
                     /translation="MRFELIKFLQQLGVNHALSPYETQPWLVYSEEKKLTCSAEVRMG
                     PTGEDIEAEIQFLHDETDDDDEDGGASSGGGREQILWMRAEPVGENQWGPKHLRIKGK
                     DYVNEFHGWEEKGCEFFLACVSALQMNEIPDIDMLIEDKMKDDSFGGGGRRGKIGRKS
                     PKVKPAALMGMKKP"
     gene            complement(1847..2488)
                     /locus_tag="PK513_01850"
     CDS             complement(1847..2488)
                     /locus_tag="PK513_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HOO81227.1"
                     /translation="MVDDILDTRFEGKEVDLADNLVYLNDDLNLMQVDPALRNVLIGA
                     GWDVNAFNADTLDLDLSVFLLNKENVTREDGDFVFYNQPETFDGGVKHRGDSRTGAGD
                     GDDESILVDLHAVPFDIIRVAICVTIYKGYEKEQNLEMLRNAYVRIVNVDTRHEICRF
                     VIDQVLQDRTETGVIIGFLNREGPKWHFKPAADFVPGGLSELGKRFGLIINQE"
     gene            complement(2557..3540)
                     /locus_tag="PK513_01855"
     CDS             complement(2557..3540)
                     /locus_tag="PK513_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOO81228.1"
                     /translation="MDVVTLQALVIPLGLIMFGTALLIKGGNWTIDSAVFIARRFGLS
                     PMVVGFTILAFGTSLPELIVSILAVLRGSEGIAMGNVIGSNIANILLVIGCSSVLVTM
                     QITLSKGLIKDLVMMLLSAFLLMGLMLYGEIGRIAGLGMVAILLMYIFVQYRMAMKGE
                     IVVDGEDLPFFKHPAQPYVFLVLGLCGVGVGAEFLVRGASQSAAILGVPEDVVALSII
                     AFGTSLPELSTSIIGARRGHAEMALGNIVGSNVFNILIIIGITALVKPIAQGSYADQL
                     LEFDIWFMLAVTVVFAALVAGYRKITPVIGGIFVSAYVVYTIYIYAINFGT"
     gene            complement(3614..4354)
                     /locus_tag="PK513_01860"
     CDS             complement(3614..4354)
                     /locus_tag="PK513_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tellurium resistance protein TerA"
                     /protein_id="HOO81229.1"
                     /translation="MDKKKTSIDSMAEASRSVVDFSGHNGSLGAAGYREASSHPENCE
                     FLNEPGSTIAISPGLEGFTSIDIGVAWDNVRLEQSGFFGKMFKKARKVGVDLDIGCLY
                     EMKDGTRGAIQAFGEKFGKFDAPPYIWLSGDERTGNAEGHDEVIHVNGAHWDDIKRIL
                     VYIYIYKGAPRWSEINPQIILDVPGENDLVVTLGAHDDALALCAVGGLESVRGGIKLT
                     NYTEYFPGHYEMDRAFGFGLEWGEGRKE"
     gene            complement(4426..5064)
                     /locus_tag="PK513_01865"
     CDS             complement(4426..5064)
                     /locus_tag="PK513_01865"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOO81230.1"
                     /translation="MVQDGLIEGADGQVRCWWCGDDPEYIRYHDEEWGRPVSNDIRLF
                     EKICLEGFQSGLSWITILRKRENFRSAFDGFDFTKIQNFDEQDIERLLGDAGIVRHRG
                     KIEATINNARRAMEMVEEFGSLAAYFWRWAPEDTDRSVSYVDIPAVTDISKALSKDLK
                     KRGWKFVGPTTVYAFMQAMGMVNDHVEGCGQCALVEEHRRAFQHFIASDSKF"
     gene            complement(5087..5671)
                     /locus_tag="PK513_01870"
     CDS             complement(5087..5671)
                     /locus_tag="PK513_01870"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO81231.1"
                     /translation="MKKATDLRILDSAEQLFWHYGHRASSLDKIANDAGQSKGAVFHY
                     FRNKKDITSQVLRKYAQENIFGLLQTHFTKTQNTKEALLNWGMEIYQSYAQDEYKGGC
                     LLGNMALELSDGDEAARHEIAKIFLEWENQLTNYFKDPARTGEIMMEPRQFARLLIAT
                     LQGVTMTAKVHKDKNRAAREFQAFAELIERMVRG"
     gene            5946..7619
                     /gene="ettA"
                     /locus_tag="PK513_01875"
     CDS             5946..7619
                     /gene="ettA"
                     /locus_tag="PK513_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOO81232.1"
                     /translation="MSAQYVYVMNGLSKSFDNKKILENVTLSFLPGAKIGVLGPNGAG
                     KSTLLKIMGGVDKDFTGEAWAADGVRVGYLEQEPQLDESKTVEENVMEALAETKAMVE
                     RFNAIGLEMAEPDADYDALMTEMGDLQEKIDAVDGWELERTISIAMDALRCPPGDSAI
                     TNLSGGERRRVALCRLLLEKPDLLLLDEPTNHLDAESVAWLQRFLSDYTGTVVMVTHD
                     RYFLDNVTGWILEVDRGSGIPYEGNYSAYLEAKRKRMEQESREEDTRQKTLKREQEWI
                     GQSPSARRSKSKARINAYQDLLAESAKLDKRSCQIVIPTPPRLGDLVIKAQNIKKGFD
                     DHLLIENLSFKLPPGGIVGVIGPNGAGKSTLFKMITGNETPDEGTFKVGDTVKLGYVD
                     QSRDSLDGDKTVWEEISGGTDIIKLGKIEMQSRAYVSAFNFRKSDQQQLVKNLSGGQR
                     NRVHLAKMLKSEANVILLDEPTNDLDTETLAALEEAIERFPGCAVIISHDRWFLDRLC
                     THILAFEGNSHVEWFEGNYQDYEADYKRRMGHDADTPHRIKYKPLRKAS"
     gene            7964..10882
                     /locus_tag="PK513_01880"
     CDS             7964..10882
                     /locus_tag="PK513_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HOO81233.1"
                     /translation="MAQIYSMVRLLPNNHPYLNPQNIGKYGIRHVIAEAANALVINHN
                     NIDQLIVFTLMLTGVVIIGTQIIMLALGLFFGSAIAASIFVTPAPTYDIAYMLMDQVF
                     GVGDGVNNFFDSCVSQNIECNPDKPPAATGAVYPWPFHLALHNLFRFYSIGILLIGTI
                     IFSYYVVVVIVETAVTGSPFGQRFKNLWVPVRLVVAVGLLIPLGLGYNSGQYITFAAA
                     KFGSSMATNGWITFNQQVAANMPGGAAGNIAGEAENLIAMPKPPDASLLAEMFSIVHG
                     CAYAHYLHDPRIAKNTAPANPPGDIRVDYPENPGQIPTYLNNRYGVKPYLVKTPTAWQ
                     AGIDPNEFLEVLPGTTYEQALQFYTSGDIVIRFGRRGDWDINGDWTISDQEEEMFKDE
                     PGQVQATCGEIRIPVTDRRPTNAGNVHATDGYLGSVAVQRMYFELIRDHWHDVNINEN
                     YIDFAGRMAMLEQNPSKKPEDSACNIGCAGGGLFSPNLDLPHNDCGAPLPPPKTEDKR
                     DCATHNISAEWKQEATNELQVTLNTKLTQIWVDYNQGTREFAVDNALLQRGWGGAGIW
                     FNKIAQANGAFMNTFFNVPEMNVYPYLMEKVKKEKGKQDSDPSGIQAYNPNTKSQSAG
                     LKLSHVESGAAPIAMIEYRVLEYWNKSNKNMAKSEKANTTNALETGMNLVFGTNGLFA
                     MNDENANIHPLAQLVALGKGLVESAIRNVAGASMGAAGGGISKALDTAAGPLADAVSG
                     LVLSTAFVGLTAGLVLFYVLPFLPFVYFYFAVASWIKTIFEAMVGVPLWALAHLRIDG
                     DGLPGESALNGYFLIFEIFVRPILSVAGLIAAMLIFTAQVRVLNFIWLMVTDNVGGHN
                     SDPTIAIAADFKFKRAIVDEFFYTVLYAIIVYMLATASFKLIDKIPDNILRWMGQGVS
                     SFGDINQDPTEGLTRYAALGGMTAGREIAGGVKDLAGGLGGVVGNVARGAATTERAAP
                     "
     gene            10952..13723
                     /locus_tag="PK513_01885"
     CDS             10952..13723
                     /locus_tag="PK513_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HOO81234.1"
                     /translation="MPGFRQRFRDLFASGFQYIPYFIALVYSSVRLLPAEHPYLNPSN
                     MGRFGIRHVLAEAANNIVFSTKNIDQILLFFCILFGLILMALQFGLLSIAIFMQPAMA
                     AMPTTFPGFFSTAASGNEAQDIANILMDMVFGVPGIFNSCVSVGVPCTTIDGNPIATA
                     AGAPGGTWSYDPTVFPFAIHRGLHQLFQLYNIGLTVVAAFIGMYFIFTIALETAESGT
                     PMGKRFNKVWAPIRFVMAFGLLFPIGYGYNSAQYIVLYAAKYGSGFATNGWNLFNDTL
                     NATYGSAFENLLNSDTASAANLVGVPNVPEVGGLLQFMYAAKVCAEAEAVAHAPPLPV
                     KFYLVGDPLKLPENHLEVNNTNVSYAQLINFADGENQVIIRFGRRDEDKYGDLMGYVH
                     PTCGELTFKLSDPRAPGTAEPGIETLQEYYWFILKELWFVTMEGNGPTPALTVPPYAQ
                     NLVKVKTFFSTAPTSYHQQGLNLHTTEWKTALQDFYRLDLREILTGTPSTPSTGILGA
                     LGTNGAAPEMRQSQRWQTDANLRDKGWAGAGIWYNRVADMNGALTTATLNIPMPSRYP
                     HVMEKVYTKKRQGEQNVDFNERFNPTSTSSAATGGLKPIEEKIAPILWEAFDFFQKGG
                     GVTTSHSAPTGNAMIDIMNTFLGTNGLFSMRQNSDVHPLAQLAGVGRSLIEASVRNLG
                     YAGIGALTGTLVKNLDMTGEAAKIASGFIITFSTIGLTAGFVLFYVVPFLPFIYFFFA
                     VGGWVKGIFEAMVGAPLWALAHLRIDGNGLAGQAAVSGYFLIFEVFLRPILIIFGLLA
                     SISIFSALVSVLNQTFDLVVANLGGFDTELELSGAAATSKVAEWRGAIDEFFFTVIYV
                     IIVYLMGMSSFKLVDMIPNNILRWMGQSVATFGDQREDAGQSLIGTATVGSQQTLGAI
                     GGGLKGLFK"
     gene            13969..16557
                     /locus_tag="PK513_01890"
     CDS             13969..16557
                     /locus_tag="PK513_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HOO81235.1"
                     /translation="MAEAANNLVFDRKHIDQIIIYFTILVGLILLGLQFILLIVAITA
                     EHPALALGITDLFPNPNTTTGSLGPEQDIAFILLDRIFGLTGFFNSCISNLGVPCTDL
                     KGVTLSSTPTAYPFPFHIALHRILQFYAYGIFWVGSLVIIYLCIAIIAETAASGTPFG
                     QRFNKAWAPVRLILFFALLVPMNWAGGPNQGLNAAQLITFKIVQLGSNMATNAWTVFN
                     GGGPSPSLSANYLSQQYDLVAQPNTPISEVNDLLQTMYLARTCAAAYHLQEGPEKKID
                     AYVVRSTPPANITPTINGATLTPNALDFNGLSYPNALDFAYRGTITIRFGRLGEAGDD
                     PAKPGELSTQYQGLKGNVKPYCGEVMIQVQGIEEPGANMIFNVYYDLVKSLWNDQQYK
                     DRSECFVKHKITEEAYDPNCSPIHDETWVKSEIAFYVNSVKLVTMGAIQIQRAQGDFA
                     VPTEIIERGWAGAAIWYNKIAAMNGAITTASLNIPKASLLPLLMETAMRNNRQQNELI
                     RTSSLFNQNMADAMGVNVLMEIKDDERNMLTAMVEAYEFWTQNGSAQQSNFDQPTGNI
                     IIDYVNSLFGTNGLFEMRNNTNVHPLAQLSALGKSMMEAVVRNAAYGAALSGVKGLGF
                     KLDQFAEGAGPAGNFLFALVIITVGIAAILYYVLPFLPFIYFMFAVSGWIKSIFEAIV
                     AMPLWALAHLRIDGEGVVGPGATNGYFLLLEIFLRPTLIIFGFVASISIFSALVNVLN
                     QIFDLVIGNLGGYDEELEANIVTGSAPAGTLSNLNIARGTVDEFFFTAMYAIICYLIG
                     LSCFKLVDLIPNNILRWMGVSVSTFQENAGDPAGKLSSNVYKGTLLIGNQVRGTAKGD
                     LAIIAD"
     gene            complement(16599..17267)
                     /locus_tag="PK513_01895"
     CDS             complement(16599..17267)
                     /locus_tag="PK513_01895"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO81236.1"
                     /translation="MQRRFLFSSVLALGLVSGIASHASLLSTSAAPVLNSRFAHFVAA
                     NAHGDEVALAAEKFVAALADQGIGFLSDKKITEEKRRAEFKALLNKNFDMNTIARFSL
                     GRYWRSATQAQRDEYVVLFKKMIVDVYSARFSDYQGQEIEVVGSRKEGERDILVHSLL
                     RQDGGPDVKVDWRVRNRSGAYQVIDVIVEGVSMAMTQRSDFSSVVQRGGGDVEALLVH
                     LRQE"
     gene            complement(17311..18108)
                     /locus_tag="PK513_01900"
     CDS             complement(17311..18108)
                     /locus_tag="PK513_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOO81237.1"
                     /translation="MFKPKAADMNNIVWNFKALVALSAMVLLVGGCASNGEDRVMAGE
                     VEVSDPFEKANRAIFSFNTAVDKAVIHPVAKGYNAVVPNPARTGIDNALKNLRSPANF
                     ANQVLQGDFDGAGNVVVRAVVNTLLGLGGLVDLAGHEGIEYEVEDFGQTLATWGVGHG
                     PYLVVPILGPSSTRDYIGYAVDSFADPLRWYLFNIEEEGIYYGKVGADYLNLRASLVD
                     VLEDLEKSSIDYYAATRSAYYQRREALVNDQGSDVITAPAIPDYDDY"
     gene            complement(18258..19385)
                     /gene="lptG"
                     /locus_tag="PK513_01905"
     CDS             complement(18258..19385)
                     /gene="lptG"
                     /locus_tag="PK513_01905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOO81238.1"
                     /translation="MKVTMTLSRYMARTYVMNMLFLLFALLGVIYLFDTVELVRRASK
                     SDSVPLGLVLQMGLLKLPEVGQILFPFAVLFSAMLTFWQFNRHSELVVLRSSGFSVWQ
                     FLMPVVAVALCVGFLQVGVINPVGSLLVEKYERLEKEYLDKQDSQIALFKEGLWLRQS
                     TDGHILEDDDDVQKGYVILHARKVKPPHWGLSQVSVFYFGQNDVFRMRADAKSASLEP
                     GRWVFEDVAIHRSGSVKISKPEMVLPTTLTIEDIEESFSSAETVPFWALPAHIETLEQ
                     TGFDASRLRVYFHSLLSQPLFFVAMVLLAASVSIRPPRMGGGLMLFAVGVFIGFVVFF
                     VSSYLQALGSSNQIPPILAAWSPAIVCFLYGVSAMIHLEDG"
     gene            complement(19382..20569)
                     /gene="lptF"
                     /locus_tag="PK513_01910"
     CDS             complement(19382..20569)
                     /gene="lptF"
                     /locus_tag="PK513_01910"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOO81239.1"
                     /translation="MKVINDILFLTRLARAKTHMNIFDRYIFKNLLIATVFVAVTLAV
                     VIFLTQSLRFLELVIESGASSSAFWVLTMLALPRFFEIILPLSLMAATLFVYSRMTSD
                     SELVVVRATGYAPSALARPAMILALIVTLLLMGITLWVAPKSISTMQEKREMIKAQFS
                     TLLFREGVFNQVGKGLTLYVRARGEAGSLVGLIIHDSRASGQPASTIFAERGTLVSAE
                     QGYRIVVYEGSRQEYDAEKGILKRLNFNRYTIDLPESDPVRQRWAEPDERTIFELLNP
                     DVRVKRDLESLRDFQVDLHRRIVSPLLAVVFTLIACSALLIGPIDRRGQMRRIMLAVA
                     SVVVIQGLYIAAYNMARQSDAGLVFMYGLVFFPLGLCSFALSGMSENLRRRVLYHWQG
                     GQA"
     gene            20596..22122
                     /locus_tag="PK513_01915"
     CDS             20596..22122
                     /locus_tag="PK513_01915"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOO81240.1"
                     /translation="MSLKISFPKSSAKPKGAIVVCAFEGGELGTQAKALDKTLKGFIT
                     ANLKNNAQFTGKNGEIMSLGAPAENTVTHITLLGLGKDQDLDALKAETAGGKLAAVLT
                     KSSTETAVLLGEKSKKLKIETLTAHLANGFALRAYDFDRYKSKPDKKDKPPLKFKTLD
                     VITPDPANAKKLFENLSHVRSGTFLARDLVNTAPNDLYPESYAKIIQNELKPLGIEIE
                     IIDDKRLEKLGMGGLSAVGQGSSRKPRMVIMRWNGVPTSKNKTQNTPTLGFVGKGVTF
                     DTGGISLKPGPNMDEMKMDMGGSAAVVGLMKTLALRKAKCNAVGVVGLAENMPSSNAY
                     RPGDIIKTYAGKTIEVLNTDAEGRLVLADCLTYIQKTYKPEFVIDLATLTGAILIALG
                     HEYCGTFANDDTLWKQMEKASEQSGEKLWRMPLDEVWTKEVESEVADLQNTGKGRNAG
                     SCTAAAFLGHFIEDGTRWAHMDIAGTAWRKSDQPTVPKFGSGFGVRVLNDLVAKYYEG
                     "
     gene            22215..23297
                     /locus_tag="PK513_01920"
     CDS             22215..23297
                     /locus_tag="PK513_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HOO81241.1"
                     /translation="MCYIKLKGGDIDTALLKKYAYRITQNIKHAIKGDITNDPIAKQY
                     LPQACELKVLPEERSDPIGDEAHSPVKGIIHRYPDRVLFKPANVCAVYCRYCFRREQV
                     GPKDKKIPGSLNPKEYTAALDYIRSHPKIWEVILTGGDPLVLSPRHLGEIMNALRAID
                     HVKVIRIHTRVPIADPARLTPALIDALASSPISPLIKANKEKTDRSVCYPDKALYMAL
                     HINHAQELTPEVRRTIKTLHQRGISLLSQSVMLKGINNDAATLANLYRELITLHVKPY
                     YLHHPDLAPGTSHFRLSIKEGQSIIRALRGHLSGLCQPTYMLDIPGGYGKVPLTPCAL
                     EPLPAGGYNVTDYKGGQHIYAPQSES"
BASE COUNT         6853 a         6690 c         4647 g         5201 t
ORIGIN      
        1 cttcggtcat agatcgtagc ttaactcact gtccgaattc ttggggccac ctctggcata
       61 tccgccacgt tcaaaaagat caattagccg atctgaaatt tcatccattc tctgcgcacg
      121 attatacgcc gtttcaacaa tctcacgagc ctcttcaacc ggcatttccg gctcaagatt
      181 gatataatcc ggcttgctct ttatccgtgc gtaaaaatga atgacctcac tcgaaagctg
      241 atcacccaaa atatcaagct tgtccgtatt gcgatcaaaa actgaacgct ccatcgctgg
      301 ctgcgcctgc acaatatcgc ccgccttttt atatttgggt tttttatccg gatctttcaa
      361 atccttaagc atctcgtcat acacgaccaa ataggcttca accttggact tgttcgcttt
      421 aatctccgaa tcaagcatcg ttgcaaaaat ttcgcgttct ttatctcgaa gcgctcgctc
      481 gcgagccatc atattcatac tttgagtctg tacgtctgcg actttttgga accccttcat
      541 aataatcaac gtcaaaaccg aaaatgcgcc gagcaaacac agaattaaaa taccgcccgc
      601 aatcatcata aaagcgccgt cattctccga agatctgctc ccttcttgca accagacacc
      661 aagcccctgc ttctgcgcat ctttctcagc ctgaatatac gattcttcaa acgcaccacc
      721 gaacacagcc gcccgatcca ccacggcaaa cccacgctga agcatataca atcccaaatc
      781 ctgatcgaaa ctattggtac attgggcaac aacgccggaa gcatcccggc ttttcatttc
      841 acagcgcacc ttattatacc ccagtacagc atcaagcgcc gcgcgaacat tcaccgaaaa
      901 ttgcgccggc atgccttcaa tcggctgaat cccccaaagc ttgattggtg ttttcccggc
      961 catcaaagtc ccgccgtcaa cagctctggc ttgagtatca aaaaaccgcg acgatgattc
     1021 agatgttttt aaagaaggcg ccccagactg cgccgcaccg gcagaaacgg gaacaacatt
     1081 tgcaattacc agcatcataa aaattacaaa gagcctaaat ttcacgcaca tcgctccgaa
     1141 aaataatcgc cttaatctca aaatcatatc actctttcat taaaaaaatg aacgctttct
     1201 aaacaaatcg gccaggcatt cccacaagca attcttgaat aactctgata aaattatgta
     1261 tggtaccaag cacgacatac aaaaccccca acacggcacg ataaatgcgt tttgagctta
     1321 taaaattctt gcaacaactc ggcgtcaatc atgcgctctc tccctatgag acacagccat
     1381 ggctcgttta cagcgaagaa aaaaaactca catgtagtgc agaagtccgc atgggaccaa
     1441 ccggtgaaga catcgaggcc gaaatccagt ttcttcacga tgaaacagat gacgatgatg
     1501 aggacggcgg cgcatcctcc ggcggcggcc gggaacaaat cttatggatg cgcgctgagc
     1561 ctgtcggtga aaaccaatgg ggacctaaac atctgcgcat taagggcaaa gactacgtca
     1621 acgagttcca cggctgggaa gaaaaaggct gcgaattttt tctcgcatgt gtcagcgccc
     1681 tgcaaatgaa tgaaatcccc gacattgata tgctgatcga agataaaatg aaagatgata
     1741 gttttggagg cggcggccgc cgtggaaaaa ttggacgaaa atcacccaaa gtaaaaccgg
     1801 ctgccctcat gggtatgaaa aaaccctagg aacaggactt attaagttac tcctgattga
     1861 taataagccc aaaacgttta cccaactcgc tcaaaccgcc aggcacaaaa tcagccgcag
     1921 gtttaaaatg ccatttaggc ccttcacgat ttaagaagcc gataattaca ccggtttcag
     1981 ttctgtcttg tagaacctga tcaataacaa aacgacaaat ttcatgacga gtatctacat
     2041 tcacaatacg aacatacgca ttacgaagca tctcaagatt ctgctctttt tcataccctt
     2101 tataaatcgt gacacaaata gcaacgcgaa taatatcgaa cggaaccgca tgcaaatcaa
     2161 caagaatact ctcatcatca ccatcaccgg cgcctgtacg gctatccccc ctatgcttta
     2221 caccgccatc aaacgtctcg ggttggttat aaaacacaaa atcgccatcc tcgcgtgtca
     2281 cattttcttt attaagcaaa aacacggaca aatcgagatc aagagtgtcg gcattaaacg
     2341 cattaacatc ccatcccgcg ccaattagaa cattacgcag agccggatca acttgcatca
     2401 ggtttaaatc atcattcaga taaaccagat tgtccgccaa atcgacttct ttaccttcaa
     2461 atcgtgtatc gagaatatca tcgaccattt gattgatccc atttcgagtt taaaaagccc
     2521 aacaccagct tccagtgtaa cacaaccgac acaaaattat gtgccgaaat taatcgcata
     2581 aatataaatc gtataaacga catacgcgct aacaaatata ccgccgatta ccggtgtaat
     2641 ttttctataa ccggcaacca acgccgcaaa aaccaccgtt acagccaaca taaaccaaat
     2701 atcaaactct aaaagctgat cggcatatga accctgcgcg attggcttca ccagcgcggt
     2761 aatgccgata attatcaaaa tattaaaaac attcgaaccg acaatattgc ccagagccat
     2821 ttccgcgtga ccgcgccgcg cgccgataat acttgtagaa agttccggca gcgacgtgcc
     2881 gaaagcaata atactcaaag ccacaacatc ctcaggaacg ccaagtatgg ccgcagactg
     2941 gctagctccg cgtaccaaaa attcagcccc aacgcctacg ccacaaagcc ctaaaaccaa
     3001 aaacacgtac ggctgggctg gatgcttgaa aaaaggtaaa tcttccccat caacaacaat
     3061 ctcacccttc atcgccatgc ggtattgaac aaatatatac atcaacaaaa tcgccaccat
     3121 acctaacccg gcaatccgtc caatttcgcc gtaaagcatc aaacccatca gcaaaaatgc
     3181 agaaagcaac atcatcacca gatctttaat caggccctta ctgagcgtaa tctgcattgt
     3241 caccaaaact gaagaacacc caatcaccag taaaatattg gcgatattcg aaccgatcac
     3301 attgcccatc gcgattcctt cagaaccccg taaaaccgct aatatagaaa caatcaactc
     3361 cggcaaagat gtaccaaacg ccaaaatcgt aaaacccacc accattggcg acagaccaaa
     3421 ccgccgtgca ataaacacag ccgaatcaat cgtccagttc cctcccttaa tcagcagggc
     3481 agtgccaaac attatcaaac ccagcggaat gacaagtgcc tgcaatgtca caacatccat
     3541 aaatatgctt tctaaaaccg aagttccctt gaagaattaa gaggaagtgc ttaaggccaa
     3601 taaaaaacca aacttactct ttccgtcctt caccccactc taacccgaaa ccaaaggcac
     3661 gatccatctc atagtgacca ggaaaatatt ccgtataatt cgtcagcttg atcccgccgc
     3721 gtacgctttc caatccacca accgcgcaca acgccaacgc atcatcatgc gcccccagcg
     3781 ttacaaccaa atcattctca ccgggcacat ccagaataat ctgcggatta atctcgctcc
     3841 aacgcggcgc tcccttatat atatatatat aaaccaaaat acgttttata tcatcccaat
     3901 gcgccccgtt cacatgaata acctcatcat gtccctccgc gttacccgta cgctcatccc
     3961 ccgaaagcca tatatagggc ggcgcatcaa attttccaaa cttctcacca aatgcctgaa
     4021 tcgcaccgcg cgtaccgtct ttcatttcgt ataagcagcc aatatcgaga tccaccccaa
     4081 cctttcgcgc ttttttaaac atcttgccga aaaacccact ctgctcaagc cgcacattat
     4141 cccaggccac accaatatca atcgaagtaa acccttccag ccccggactg atagcaatcg
     4201 tcgaaccagg ttcattcaaa aattcacaat tctcggggtg cgaagaggcc tcccgataac
     4261 cggcagcccc caaagaaccg ttatgaccag aaaaatctac aacgctacgc gaagcttcag
     4321 ccatcgaatc aatagaggtt tttttcttat ccatattcac tccataaaca atacgaaacc
     4381 atagcactgc aatccctaaa agccaaaaga ataaaggact taggcttaga atttagaatc
     4441 agaagctata aaatgctgaa acgcccggcg atgctcttca acaagcgcac actgcccgca
     4501 cccttcaaca tgatcattaa ccattcccat cgcctgcata aacgcataca cggttgttgg
     4561 cccaacaaat ttccagccac gctttttcaa atctttcgaa agcgcttttg aaatatccgt
     4621 caccgctgga atgtccacat aagataccga ccggtcggta tcttccggcg cccacctcca
     4681 aaaataagcc gctagcgaac caaactcttc taccatttcc atggcgcggc gcgcattatt
     4741 gatggtggct tcaattttgc cccgatgacg aacaatcccc gcatcgccga gcaaacgctc
     4801 aatatcttgc tcatcaaaat tttgaatttt agtaaagtca aacccgtcaa atgcagagcg
     4861 aaaattttca cgcttacgca aaatcgtaat ccagctcaaa ccggactgaa acccctccag
     4921 acaaatcttc tcaaacaacc gaatatcatt gctaacgggc cgcccccact cctcatcgtg
     4981 ataacgaata tattcaggat catcaccgca ccaccagcaa cggacctgcc catcagcgcc
     5041 ttcaatcaaa ccgtcttgaa ccatatcaaa aaaccgaccg gtcggtttaa ccgcgaacca
     5101 tgcgctcaat taattccgca aacgcctgaa actcacgcgc tgcacgattt ttatccttat
     5161 gaaccttggc cgtcatcgta accccctgca acgtcgcaat caacaaccgc gcaaattgcc
     5221 gcggctccat cataatctca cccgtgcgcg ccggatcttt gaaataattg gtcagttgat
     5281 tttcccactc caagaaaatc tttgctattt catgacgcgc cgcctcatcg ccgtccgata
     5341 gctccagcgc catattacca agcaagcacc cacccttata ttcatcttgc gcataagact
     5401 gataaatctc catcccccaa ttcaaaagcg cctctttggt attttgcgtc ttggtaaaat
     5461 gcgtctgcaa caatccaaaa atattttcct gcgcatattt gcgcaaaacc tgcgaagtaa
     5521 tatccttttt gttgcgaaaa taatgaaaca ctgcgccctt gctctgaccc gcatcattgg
     5581 caattttatc gagcgaactc gcccggtgcc cgtaatgcca aaacaattgc tcagcgctat
     5641 ccaatatccg taaatctgtt gcttttttca tccctgaatc ctcctgcccc acaaatatac
     5701 cgaccggtcg gtttttcatc aagccttact tctaaaatgc cctcaacttt tcccgcgaaa
     5761 tacgctgcca agcgcttacc ttgtctatac aaccagtgaa aaaccacgac atgaacgttc
     5821 tttcaaagcc tttcacaccc accgcatctc cgacgatgaa gcctttttcc aagatcgctt
     5881 ctaaagattg atttgcacgc agttctgcgc taactctatg cttctaaaat cacaaaggag
     5941 taaaaatgtc agcccagtac gtctatgtta tgaacggctt gtctaaatct ttcgacaaca
     6001 aaaagatcct tgaaaacgtt acactcagct tcttgcccgg cgcaaagata ggtgtgctcg
     6061 gccccaacgg cgcgggtaaa tccaccctgc tcaaaatcat gggcggcgtt gataaagatt
     6121 tcacaggcga agcttgggcc gccgatggcg tacgtgtcgg ctatctcgag caggaaccac
     6181 aactcgacga aagtaaaacc gttgaagaaa acgtcatgga agccctggcc gaaaccaaag
     6241 ccatggtgga acgtttcaac gcgatcggcc tggaaatggc cgagcccgac gccgactacg
     6301 acgctctcat gaccgaaatg ggtgatttac aagaaaaaat cgacgctgtt gatggctggg
     6361 aattagaacg tacaatttcc atagctatgg acgctctgcg ctgcccaccg ggcgattcag
     6421 ccatcaccaa cctgtccgga ggagagcgcc gccgcgtcgc cctgtgccgt ctgctgctcg
     6481 aaaaaccgga cttgctactt ctcgatgagc caaccaacca cctcgatgcc gaaagcgtcg
     6541 cgtggctcca acgcttctta agcgattaca ctggcaccgt cgttatggtc acccacgacc
     6601 gctacttcct cgacaacgta acaggctgga ttctagaagt cgaccgcggc tctggcattc
     6661 cctacgaagg caattactcc gcctatctgg aagccaagcg caagcgcatg gaacaggaat
     6721 cacgcgaaga agatacgcgc caaaaaacac tcaaacgtga acaggaatgg ataggacaaa
     6781 gcccatctgc gcgccgttcc aaatcaaaag cccgaatcaa cgcctaccaa gaccttttgg
     6841 ccgaaagtgc caaactcgac aagcgcagtt gccaaatcgt cattcccacc ccgcctcgtc
     6901 ttggcgatct tgttatcaaa gcacaaaaca tcaaaaaagg ctttgacgat cacctgctca
     6961 ttgaaaatct ctccttcaag ctcccccccg ggggcatcgt cggtgtcatc ggccccaacg
     7021 gcgcgggtaa atccacgctc tttaaaatga tcaccggtaa tgaaaccccg gacgaaggca
     7081 ccttcaaagt cggcgacact gtcaagctcg gctatgtcga ccaaagccgc gattcccttg
     7141 atggcgataa aaccgtctgg gaagaaatct ccggtggcac cgacatcatt aaactcggta
     7201 aaatcgagat gcaaagccgg gcgtacgtgt cggccttcaa cttccgtaaa tccgaccagc
     7261 aacaactggt taagaatctt tctggcggcc agcgcaaccg tgtacatctg gctaaaatgt
     7321 tgaaatcaga agccaatgtc atcctgctcg acgaaccgac caacgatttg gacaccgaaa
     7381 ccctcgccgc actcgaagaa gccatcgaac gcttcccggg ctgcgccgtc atcatctccc
     7441 acgaccgctg gttccttgat cgcctctgta cgcatatcct tgctttcgaa ggcaacagcc
     7501 atgtcgaatg gtttgaaggc aattatcagg actacgaagc cgattacaaa cgccgtatgg
     7561 gccatgatgc cgacacgccc caccgcatca aatacaaacc tttgcgcaaa gccagctaac
     7621 tgccctaaag caccgagctt aaaataccat ataaaaagcc tgtaaaacca aatctttaca
     7681 ggtttttaag gcaattgtga caaaattgtg tctgagcaaa acatgtataa aaaaacaaca
     7741 tgttatagaa ataaacaact ttataatggg gcgaaaacca taaaatacga acaaattaac
     7801 taaaatttta actattttcg cgctacacta tatattgaag tggtgagtat ttgacgtgag
     7861 cggtataaaa gcaaaaacag ttttgaaata cgcaataatg ccggagataa tcccccggct
     7921 aaagcgcctt gttacgtccg gcttcggcta tattgccttc ctaatggcac aaatttattc
     7981 aatggttcgc ctgctgccga ataaccaccc ttatctgaac cctcaaaata ttggcaaata
     8041 cggcatccgc cacgtcatcg ccgaagccgc caacgcattg gtcatcaacc ataataatat
     8101 cgaccaattg attgttttca cgctcatgct caccggcgtt gtgataatag gcacacaaat
     8161 aattatgctg gcgctcggcc tgtttttcgg ctcggctata gccgcttcaa ttttcgtaac
     8221 gcccgcaccg acatatgata ttgcctacat gctcatggac caggttttcg gcgttggaga
     8281 cggcgtaaac aacttttttg attcctgcgt atcgcaaaat attgaatgca acccggataa
     8341 accgccagca gcaacgggcg cagtataccc ctggccattc catcttgcgc tccataattt
     8401 atttagattc tactccattg gcatccttct gatcggaacc atcattttct cttactatgt
     8461 ggtcgtcgtc atagtcgaaa cggcagtaac aggctcgccc ttcggtcaac gtttcaaaaa
     8521 cctctgggtg ccggtacgcc tggtcgtcgc tgtaggattg ttgatccctt taggtttagg
     8581 atacaactca ggacaatata ttaccttcgc tgccgccaag ttcggctcaa gcatggccac
     8641 gaatggctgg atcacgttta accagcaggt tgccgcaaac atgcccggtg gcgcagcagg
     8701 aaacatcgcc ggcgaagcgg aaaacctgat cgccatgcca aagcccccgg atgcctcttt
     8761 actcgccgag atgttctcaa tcgtgcacgg ctgcgcttat gcgcattatc tgcacgatcc
     8821 acgcattgcg aaaaacacag cgccggcaaa cccgcctgga gatatacgag tcgattaccc
     8881 cgaaaacccg ggacaaatac ccacttattt gaacaaccgc tacggcgtaa aaccttatct
     8941 ggtcaaaacc ccgaccgcct ggcaggcagg catcgatccc aatgaatttc ttgaagtcct
     9001 gcccggaaca acatatgaac aggcgctgca attttacacc agcggcgaca tcgtcatccg
     9061 ctttggccgt cgtggcgatt gggacattaa tggtgactgg accatatccg accaggaaga
     9121 agaaatgttc aaagacgaac ctggacaggt ccaggcaacc tgcggtgaaa tccgcatccc
     9181 tgtaacagac cggcgcccga ccaacgctgg taacgttcac gccacagacg gatatctggg
     9241 gtctgtcgcc gtccagcgca tgtattttga actcatccgc gaccactggc acgacgtaaa
     9301 tataaacgaa aactacatcg attttgccgg ccgcatggcg atgctcgaac aaaacccgtc
     9361 aaaaaaacct gaagattcag cctgtaatat cggctgcgca ggtggcggct tattctcccc
     9421 aaaccttgac cttccgcata acgattgtgg cgccccgctt cctcctccca aaacagaaga
     9481 caagcgtgat tgcgcgaccc acaacatctc ggcagagtgg aaacaagaag caacaaacga
     9541 attacaagtt acgctcaaca ccaaactaac ccaaatttgg gtcgactata atcagggcac
     9601 cagagaattc gctgttgata atgccctgct ccaacgcggt tggggcggcg caggtatatg
     9661 gttcaacaaa atcgcccaag ccaatggcgc tttcatgaac accttcttca acgtaccgga
     9721 aatgaacgtt tatccatatt taatggaaaa ggtaaaaaag gaaaaaggta aacaagacag
     9781 cgacccttcc ggcatccagg cctacaaccc taacaccaaa tcacaaagcg caggccttaa
     9841 actaagccac gtggaatcag gcgctgcccc aattgcgatg attgaatatc gtgtccttga
     9901 atactggaat aaaagtaaca aaaacatggc aaaaagcgaa aaagccaata ccacaaacgc
     9961 ccttgaaacg ggtatgaatc tcgttttcgg aacaaacggc ttattcgcca tgaacgacga
    10021 aaacgcaaac atccacccgc tggcacaact cgtcgcgctc ggtaaaggcc ttgtcgaatc
    10081 cgccatccga aatgttgcag gagcctctat gggcgctgct ggtggtggta tttccaaagc
    10141 gctcgacacc gcggccggtc cgttagctga cgccgtcagc ggcctcgtat tatctactgc
    10201 tttcgtcgga ctgacagcag ggctcgtact cttttacgtc ctgccattcc tgccatttgt
    10261 atatttctac tttgctgtag cctcatggat taaaaccatt ttcgaagcaa tggttggcgt
    10321 accgctatgg gccttggcac acttaagaat tgacggcgat ggactgcccg gcgaatccgc
    10381 cttaaatggg tatttcctga tttttgaaat ctttgtacgt ccgattttgt cggtcgcagg
    10441 cctcatcgcc gccatgctga tcttcaccgc acaagtacga gtcttgaact tcatctggtt
    10501 gatggtcacg gacaatgtcg gcggccataa cagcgacccg acaatcgcca ttgccgcaga
    10561 cttcaagttc aaacgtgcca ttgtcgatga atttttctat accgttttat atgcgatcat
    10621 cgtatatatg ctcgcaaccg cgtccttcaa actgatcgat aaaattccgg acaacatcct
    10681 gcgctggatg ggacaaggcg tctcatcctt cggcgacatt aaccaggacc cgacagaagg
    10741 cctgacccgc tatgccgctc tgggtggtat gaccgctggc cgcgaaattg ctggcggagt
    10801 taaagatctg gccggtggtc ttggaggcgt tgtaggaaac gtcgcaagag gtgcagctac
    10861 cacagaaaga gctgctccat agagtaagaa ggaggaaaga aatttaaaca tgcaaagtgt
    10921 aacgcgaaaa aatataataa aatacgcagt aatgccgggc tttcggcagc gttttcgcga
    10981 tctcttcgcc tccggcttcc agtacattcc ttatttcatc gcccttgtat acagctcggt
    11041 gcgtctttta cctgcagagc acccctacct gaacccttca aatatgggac gtttcggcat
    11101 ccgtcacgtt ctggccgaag ccgccaataa cattgttttt tcaacaaaga acatcgacca
    11161 gatcttatta ttcttctgta tcctgtttgg cctcatatta atggctttac aatttggttt
    11221 gctgagcatc gccatattta tgcaaccggc aatggctgca atgccaacaa cctttccagg
    11281 atttttctca acagcagcct caggcaatga agcacaagac atcgccaaca tattgatgga
    11341 tatggtcttt ggcgtcccgg gtatcttcaa ctcctgtgtt tcagtcggcg taccctgtac
    11401 cactattgat ggaaacccta ttgcaaccgc cgcaggcgca ccgggcggaa catggtcata
    11461 tgacccgaca gtattccctt tcgccattca tagaggccta caccaactct tccaacttta
    11521 taatatcggc ctcaccgtgg ttgcagcatt cataggaatg tattttattt tcacaatcgc
    11581 tctggaaacg gcggaaagcg gaaccccgat gggcaaacgt tttaacaagg tctgggcgcc
    11641 gatccgcttt gtcatggctt ttggcctgct ttttccaatc ggctatggat ataactcagc
    11701 gcaatatatt gtgctttatg ctgcaaaata cggctcgggt tttgcgacca acggctggaa
    11761 cctgtttaac gacacattaa acgccacata tggcagcgcc tttgaaaacc ttctgaacag
    11821 tgacacggcc tcagccgcca accttgtggg cgttcccaac gtcccggaag ttggcggact
    11881 tttacaattc atgtatgccg caaaagtatg cgcagaagct gaagccgttg cccatgcccc
    11941 ccctctgccc gttaaatttt acctggtcgg cgatccttta aaactgcccg aaaatcatct
    12001 cgaggtaaat aacacaaatg tctcatatgc gcagctcata aattttgcag acggcgaaaa
    12061 ccaggtcatc atccgcttcg gacgccggga tgaagacaaa tacggcgacc tcatgggcta
    12121 tgtccacccg acctgcggcg aactgacatt taaactttct gatcccagag cccccggcac
    12181 ggcagagcct ggcatcgaaa cactacagga atactactgg tttatcctca aggaattatg
    12241 gttcgtaacc atggaaggca atggtccaac cccggcattg acagtgccgc cctacgccca
    12301 gaatttagtc aaagtaaaaa cattcttttc aactgcaccg accagttacc atcaacaagg
    12361 cttaaacctc catacaactg aatggaaaac cgccctgcag gacttctacc ggcttgacct
    12421 gcgtgaaata ttaaccggta ctccaagtac gccatccaca ggcatcttgg gcgcgttggg
    12481 caccaacggc gccgcaccgg aaatgcgcca gtcccagcgt tggcaaactg acgccaatct
    12541 gcgtgacaaa ggctgggccg gcgccgggat ctggtacaac cgcgttgccg acatgaacgg
    12601 cgcgctgaca acggccacgc tcaacattcc gatgccctca cgctacccgc acgtcatgga
    12661 aaaagtatac acaaaaaaac gccagggcga acaaaacgtt gactttaatg aacgctttaa
    12721 tccaacatca acctcaagtg ctgccacagg agggctaaag cccatagaag aaaaaatcgc
    12781 ccccattcta tgggaagcct ttgatttctt tcaaaaaggc ggcggcgtca caacatcgca
    12841 ctccgccccc acaggcaacg caatgatcga cattatgaat acgtttctgg gcacaaacgg
    12901 gctgttcagc atgcgtcaaa actccgacgt acatcccctg gctcaactgg ccggcgttgg
    12961 ccgctcgctt atcgaagcct cggttcgaaa cctcggctat gccggtattg gcgcactgac
    13021 aggtacatta gtaaagaatc tggacatgac cggagaagcg gcaaaaattg catcaggctt
    13081 catcataaca ttttcaacaa tcggcctgac cgctggattc gtcctgttct acgtagtccc
    13141 cttcctgccc tttatctact tcttctttgc agttggcgga tgggtaaaag gtatttttga
    13201 agctatggtc ggcgccccgc tttgggcttt ggcgcatttg cgaatcgacg gcaacggctt
    13261 ggccggacaa gccgcggtca gtggctattt cctgattttc gaagtctttt tacgacccat
    13321 tttaatcatc tttggactgc tggccagcat ctcgattttc tcggcgctgg tcagtgtatt
    13381 aaatcaaaca tttgatctgg tcgttgctaa ccttggcggt tttgataccg aactcgaact
    13441 ctcaggcgcc gccgcaacct caaaagtcgc agaatggcgg ggagcaattg atgaattctt
    13501 cttcacggtt atctacgtca tcatcgttta tctgatggga atgtcctcct ttaaactggt
    13561 cgacatgatt ccaaacaaca tcctgcgctg gatgggacaa tctgttgcaa catttggaga
    13621 ccagcgcgaa gatgcaggtc aaagcttgat cggaacggca accgtcggct cacagcaaac
    13681 acttggtgct atcggcggcg gactaaaagg actgtttaaa taacaaaaga tagaaacgta
    13741 caaaaaacaa cgtttaaaga tgaaaatgga taaatgggaa cacaaagtaa aatcctgaaa
    13801 tatacgctcc tccccgggtt cattccccgg atttctgcgc ttttcaaaaa cggttttaca
    13861 catttggctt atacgatagc gctaattttt agcaatgtcc gccttctccc cacaggacac
    13921 ccttatctga accctcaaaa tataggtcag ttcggaattc gccatgtcat ggccgaagcg
    13981 gcaaacaacc tcgttttcga ccgaaaacac atagatcaga tcattattta ttttacaatt
    14041 cttgtggggt taattttatt aggactgcaa ttcatacttc tgatcgtcgc gatcacggcc
    14101 gaacatcctg cccttgcgtt aggcatcacc gacctgttcc caaacccgaa tacaacaacc
    14161 ggttcactcg gcccggaaca ggatatcgcg ttcatccttc tggaccgaat ttttggacta
    14221 acaggttttt ttaactcctg catctccaat cttggtgtac cttgcacaga tctaaaaggc
    14281 gttactttat ccagcacacc caccgcttac cctttcccct tccatatcgc cttgcaccgc
    14341 atcttacagt tttatgctta tggaattttc tgggttggtt ctcttgtcat catatatctg
    14401 tgtattgcaa taatcgctga aaccgccgct tcaggaacgc ccttcggaca acgctttaat
    14461 aaagcctggg cccccgtcag attaatttta ttttttgccc tgcttgtccc tatgaactgg
    14521 gcaggcggcc ccaatcaggg tctcaacgca gcccagttaa tcacttttaa aatagtgcag
    14581 ctcggctcca acatggcgac aaatgcctgg acggtcttta atggcggagg gccatcacca
    14641 agcctgtctg caaactattt aagccagcaa tatgatctgg tggcccagcc caacacaccg
    14701 atttcagaag tcaacgacct gctccaaacg atgtacctcg cccgaacctg cgctgcagct
    14761 taccaccttc aggaaggccc cgaaaaaaaa atagatgctt atgtagtgcg ttccactccc
    14821 ccggcaaaca taaccccaac cataaacggg gcaacgctta caccaaatgc gctcgatttt
    14881 aacggcttaa gctaccccaa cgcccttgat tttgcctatc gtggaacaat aaccatacgc
    14941 ttcggccgct taggtgaagc tggagacgac cccgcaaaac ccggagaact ctcaacacaa
    15001 taccaaggac tcaaaggaaa cgtaaaaccc tactgtggtg aagtcatgat acaggttcaa
    15061 ggcattgaag aacccggtgc aaatatgatt ttcaacgtat actacgactt ggtaaaaagc
    15121 ctgtggaatg atcaacaata taaagaccga tccgagtgtt ttgtaaaaca caaaatcaca
    15181 gaagaagctt acgatccaaa ctgcagcccc attcacgacg aaacctgggt aaaaagcgaa
    15241 attgcatttt acgtaaactc tgtaaaactc gtcactatgg gcgcaatcca gattcaacgc
    15301 gcacaaggcg acttcgctgt gccaaccgaa atcatcgaac gcggctgggc cggcgcagcc
    15361 atttggtata acaaaatcgc agcaatgaac ggtgccatca ccaccgcctc actcaatatc
    15421 cctaaagcat cactcctccc acttctcatg gaaaccgcaa tgagaaataa cagacagcaa
    15481 aatgagctca tcagaacaag tagcctcttc aaccaaaata tggcagacgc tatgggcgtc
    15541 aatgtcctca tggagataaa agacgacgag cgcaacatgc taaccgctat ggttgaggcc
    15601 tacgaattct ggacacaaaa cggttctgca caacaatcaa acttcgatca accaacaggc
    15661 aacatcataa tcgactacgt caattccctg tttggtacaa acggcctgtt tgaaatgcgc
    15721 aacaatacaa acgtacaccc actggcacaa ctctcggctc tcggtaaaag catgatggaa
    15781 gccgtagtac gcaacgctgc atacggtgct gcactaagcg gcgtcaaagg gcttggtttt
    15841 aaattggatc aattcgcaga aggcgctggc cccgccggaa acttcctgtt cgctttggtt
    15901 atcattacag tcggcatcgc agcaattcta tactacgttc tgccattcct gcccttcatt
    15961 tactttatgt tcgccgtcag cggatggatc aaaagcatat ttgaagccat cgttgccatg
    16021 ccgctatggg cacttgcaca tttacgcatt gatggtgaag gcgttgtcgg cccgggtgca
    16081 acaaacggat acttcctgct actggaaatc ttcctgcgcc caacccttat catcttcggg
    16141 tttgtggcca gcatcagcat attctcagcg ctggtaaatg tattaaacca gatttttgat
    16201 ctcgtcattg gcaatttggg aggctatgac gaagagctcg aagccaatat agtaaccggc
    16261 agcgcaccgg caggaacact ttccaacctt aatatcgccc gaggcacagt agatgagttt
    16321 ttcttcaccg caatgtacgc cattatctgc tacctgatag gactatcctg cttcaagctg
    16381 gttgacctta ttccaaacaa catcctgcgc tggatgggcg tttcagtttc aaccttccag
    16441 gaaaacgcag gcgatccggc tggaaaacta tccagcaatg tatataaagg aacgctgctt
    16501 atcggaaacc aggtccgcgg caccgccaaa ggcgacctgg caatcatcgc agattaaaaa
    16561 acgaaaaata cactcttaaa acttgagcag gaaacaacct attcttggcg caaatgcacc
    16621 agaagagctt ctacatcgcc gccaccgcgt tgcacgacag aagaaaaatc agagcgctgc
    16681 gtcatcgcca tgctcacacc ctccacaatc acatctataa cctgataagc gccgctgcga
    16741 ttacgaacac gccagtcaac tttgacatcc ggcccaccat cttgccgaag aagtgaatgc
    16801 acgagaatat cgcgttcacc ttctttacgc gaccctacaa cttcaatttc ctgcccctga
    16861 taatcgctaa aacgcgcaga ataaacatcc acgatcatct ttttaaacaa caccacatac
    16921 tcgtcacgct gcgcctgcgt agcactgcgc caataccgcc ccaatgaaaa acgggcaata
    16981 gtgttcatat caaaattttt attcaataac gctttaaatt cggcgcgccg tttctcttcc
    17041 gtaattttct tatcagaaag aaaccctatg ccctgatctg ccaatgcagc aacaaatttt
    17101 tcagccgcca aagccacctc atccccgtgc gcattagcag ctacaaaatg cgcaaacctg
    17161 gaatttaaaa ctggagcagc tgaagtgctc aaaagcgaag catgagaagc gatcccggaa
    17221 accagaccca acgccaaaac actcgaaaac aggaatctac gttgcatgat caaagagcct
    17281 aaattcattt aatagcctaa aacacgcatc ttaataatca tcataatcag gaatagctgg
    17341 cgcagtaatc acgtcagacc cctgatcatt aaccaaagcc tcgcgacgtt gataatacgc
    17401 actgcgcgta gcagcataat aatctataga acttttctca agatcttcca gaacatcaac
    17461 caaagacgcc cgcaaattca ggtaatctgc accgacctta ccgtaataaa taccctcttc
    17521 ttcaatatta aagagatacc agcgcaacgg atcggcaaaa gaatcaaccg catatccgat
    17581 ataatcgcgt gtagaagacg gccctaaaat tggcacgacc aaatacggcc catgaccaac
    17641 gccccatgta gcaagcgttt gcccaaaatc ttcaacttcg tattcaatac cctcatggcc
    17701 ggccagatca accaagccac caagacccaa aagcgtattt acaacggcac gcacaaccac
    17761 attgcccgca ccgtcaaaat caccttgcaa gacctgattc gcgaaattcg caggactacg
    17821 cagattcttc agcgcattat caatacctgt acgtgcagga ttcggcacaa ccgcattgta
    17881 ccctttggca acaggatgaa taaccgcttt atccaccgca gtattaaacg agaaaattgc
    17941 ccggttagcc ttctcgaacg gatcgctaac ttcaacctca ccagccataa cgcggtcttc
    18001 accatttgat gcgcacccgc caacaagcaa aaccatcgct gataaggcta ccagtgcttt
    18061 aaaattccat acaatattat tcatgtctgc cgctttaggt ttaaacatag atccgacctt
    18121 ttataagtga ttgaaagaag tacgcaagtc aagaatggaa acttattttt cactatttaa
    18181 ccagcgcaaa cttattaaaa ggttaacgcc accgtaaaaa tgttaaaaaa taaaatatta
    18241 taaaaaataa ccacgatcta accgtcttca agatgaatca tcgcactcac cccatataaa
    18301 aaacatacaa ttgccggaga ccacgccgcc aaaatcggcg gaatttgatt ggaagagcca
    18361 agcgcctgta aataactgga aacaaagaac acgacaaaac cgataaacac gcccacagcg
    18421 aagagcatca aaccgccacc cattcgcggc gggcggatag aaacgcttgc cgcaagcaag
    18481 accatagcta caaaaaacaa aggctgagac aacaaactat gaaaatatac ccgcaaacgg
    18541 ctggcatcaa aaccggtctg ctccaatgtc tctatgtgcg ccggaagcgc ccaaaacggt
    18601 actgtttcag cagaagaaaa actctcctca atatcttcaa tcgtaagtgt cgtcggtaaa
    18661 accatctctg gcttactgat cttcacggat ccggacctat gaatagcaac atcctcaaac
    18721 acccagcgcc ccggctctaa agaagcgctt ttggcatccg cgcgcatgcg aaatacgtca
    18781 ttctgcccaa aataaaatac gctcacctga ctaagccccc aatgcggcgg cttaacctta
    18841 cgcgcatgaa gtatcacata ccccttttgt acatcgtcat catcttccag aatatgacca
    18901 tccgtactct gacgaagcca aagtccctct ttaaacaacg caatttggct atcttgctta
    18961 tcaagatact ctttttccaa ccgttcatat ttttccacca gcaatgagcc aaccggattg
    19021 atgaccccaa cttgtaaaaa accaacacac aaggccaccg ccaccacagg catcaaaaac
    19081 tgccagactg aaaaaccgga agaacgcagc acaaccagct cagaatggcg attaaattgc
    19141 caaaatgtca gcatcgcact aaacaaaaca gcaaaaggaa acaaaatctg cccaacctcg
    19201 ggcaatttca ataaccccat ctgcaacacc agtcccaacg gaacagaatc gcttttactg
    19261 gcccggcgaa ccaattccac cgtgtcaaac agataaatca cccctagcaa ggcaaagagc
    19321 aaaaaaagca tattcataac ataagttcgc gccatataac ggctaagcgt cattgtcacc
    19381 ttcatgcctg tcctccttgc caatgataga gcacccggcg gcgtaaattc tcactcatcc
    19441 cgctcaaggc aaaagaacac aacccaagcg gaaagaatac cagcccatac ataaacacca
    19501 gccccgcatc actttgccgc gccatattat aggccgcaat atacagcccc tgaatcacca
    19561 cgacgcttgc cacagccagc ataatgcgcc gcatctgccc gcgccggtca atcggcccga
    19621 tcaacagcgc gctacatgcg ataagcgtaa ataccaccgc caaaagcgga ctgacaatcc
    19681 gccgatgaag atcaacctga aaatcgcgta aactctccaa atcgcgtttc acacgtacat
    19741 ctggattaag cagctcaaaa atcgtccgct catccggctc cgcccaacgc tggcgcaccg
    19801 gatcactctc aggcaaatca atcgtataac gattaaaatt cagccgtttc agtatccctt
    19861 tttcagcatc atattcctga cgcgatccct catacacaac aatcctgtaa ccctgctcag
    19921 cagaaaccag cgtcccgcgt tcggcaaaaa tcgtcgaagc tggttgcccg gaagcgcggc
    19981 tatcatgaat tatcaagccc acaagcgatc cggcttcacc acgcgcccgc acataaagcg
    20041 tcaacccttt gcccacctga ttaaaaacac cttcacggaa aagcagcgtc gagaactgcg
    20101 ccttaatcat ctcgcgcttt tcctgcatcg tgctaattga ttttggtgca acccagagcg
    20161 taatccccat caataaaagc gttacaatca acgccagaat catggccggc cgcgccaaag
    20221 cagaaggcgc ataaccggtt gcgcgcacca ccaccaactc agaatcactg gtcatgcggc
    20281 tataaacaaa caacgtcgcc gccatcaacg acaacggcaa aataatttcg aaaaaccgtg
    20341 gcaaagccag catcgtcaaa acccaaaacg cgctgctcga agccccggat tcaatgacca
    20401 gctccaagaa ccgcaaggat tgcgtcaaga aaatcaccac cgccagcgtc acggcaacaa
    20461 acaccgtggc aatcaaaaga tttttaaaaa tatagcgatc aaaaatattc atatgcgttt
    20521 tggctctggc caatctcgtc aaaaacagta tatcgtttat aaccttcatc cacatataag
    20581 agaagatgaa agacgatgag cctaaaaatt tcattcccta aatccagcgc aaaacccaaa
    20641 ggcgcaattg ttgtttgcgc ctttgaaggt ggggaactgg ggacacaagc caaagcgctg
    20701 gacaaaacac ttaagggctt cattaccgca aaccttaaaa acaacgcaca atttaccggt
    20761 aaaaatggtg aaattatgag tctcggcgcc ccggcagaaa acacagtgac acatatcaca
    20821 ctgctcggtc tgggcaaaga tcaagatctg gacgcgctaa aagcagaaac agccggtggt
    20881 aaactggcgg ccgtacttac caaatccagc actgaaactg ccgtactgct cggcgaaaaa
    20941 agtaaaaaac tcaaaattga aaccctcacc gcgcatcttg ccaatggctt tgccctgcgc
    21001 gcctatgatt tcgaccgtta taaatcaaaa cctgataaaa aagataaacc gccgctcaaa
    21061 ttcaaaacct tagacgtcat aacgccagat ccggccaacg ccaaaaaact gttcgaaaat
    21121 ctaagccacg tgcgttcagg cactttccta gcccgcgatc tggtcaatac agcgcccaac
    21181 gacctctacc cggaaagcta cgccaaaatt atccaaaacg agctcaaacc actcggtatt
    21241 gaaattgaaa ttatcgacga taaaaggctc gaaaaacttg gcatgggagg cctttccgcc
    21301 gtcggccagg gctcaagccg caaaccgcga atggtcatca tgcgctggaa tggcgtccca
    21361 acttcaaaaa acaaaaccca aaacaccccc acccttggct ttgtcggcaa aggcgtcacc
    21421 tttgacaccg gcggcatctc gcttaaacct ggcccgaaca tggatgaaat gaaaatggac
    21481 atgggcggct cggctgccgt tgttggcctg atgaaaactc tggccctgcg gaaggccaaa
    21541 tgcaatgctg tcggcgttgt cggcctggcc gaaaatatgc catcctcaaa cgcgtacaga
    21601 cccggcgaca tcatcaaaac ctacgccgga aaaaccatcg aagtcctcaa cacagatgcg
    21661 gaaggccggc tcgttctggc cgattgccta acctatatac aaaaaacata caaacccgaa
    21721 ttcgtcattg atctggccac cttaaccggc gcaattctta tcgcactagg ccacgaatat
    21781 tgcggcacct ttgccaatga cgataccctg tggaaacaaa tggaaaaggc cagcgagcaa
    21841 agcggcgaaa aactctggcg catgcctctt gatgaagtct ggacaaaaga agtcgaaagc
    21901 gaagtcgccg acctgcaaaa taccggcaaa ggccgcaatg ccggctcttg caccgccgca
    21961 gccttcctcg gccatttcat tgaagatggc acacgctggg cacacatgga cattgccggc
    22021 accgcctggc gcaaatccga ccagccaacc gtgcccaaat tcggctccgg cttcggtgta
    22081 cgtgtcctca acgaccttgt cgcaaaatat tatgagggct gatgcctaaa cacttcgcat
    22141 cgcaaacgac tcaaaatccc gacgagccaa aagcctcttc accgccttac accccacatt
    22201 cctccttctt gaaaatgtgt tatataaaat taaaaggcgg agatatagat accgcattac
    22261 taaagaaata cgcctatcga ataacgcaaa acatcaaaca tgcgatcaaa ggcgatatta
    22321 caaacgaccc catcgccaaa caatatctcc cgcaagcctg tgaactgaaa gtcctgcccg
    22381 aagaacgctc agacccgatc ggcgatgaag cccatagccc cgtaaaaggc atcatccacc
    22441 gctatccaga ccgggtattg ttcaaacccg ccaatgtctg cgcagtttat tgccgttact
    22501 gttttcgccg cgaacaagtc ggcccgaaag acaaaaaaat ccccggcagc ctgaacccta
    22561 aagaatacac cgccgcactc gattatatcc gttcgcaccc caaaatctgg gaagtcatcc
    22621 tcaccggcgg cgatccacta gtgctctcgc cgcgtcatct aggtgaaatc atgaacgcgc
    22681 tgcgcgccat cgatcacgtc aaagtcattc gtatccacac ccgtgtgcct atcgctgatc
    22741 cagcccgcct cacgcccgcc ctgatagacg ctctagcaag ctctccaata tcccctctca
    22801 tcaaagcgaa caaagaaaag accgaccggt cggtatgcta ccctgataaa gcgctctata
    22861 tggccctgca tatcaatcat gcccaagaac ttacaccaga agttcgccgc acaataaaaa
    22921 cactccacca acgcggcatc tcgctgctct cacaatccgt catgctcaaa ggcattaata
    22981 atgacgccgc cactctcgca aatctttacc gcgaattgat caccctgcat gttaaaccct
    23041 attacctcca ccatcccgat cttgcccccg gcaccagcca tttccgcctc tcgatcaagg
    23101 aaggacaaag cattatacgc gccctgcgtg gccatctctc cggcctgtgt caaccaacct
    23161 atatgctcga catccctggt ggctacggca aagttcccct caccccttgc gcccttgaac
    23221 ctttaccggc aggcggctac aacgttacag attataaggg cgggcagcat atctatgcac
    23281 cgcaaagcga aagctaaaaa ccatgactga aattcgtttc tatcaccttg aacgcaccga
    23341 actagaacag gtcctgccac aactggtcac caaagccctg agcgctgacc a
//
LOCUS       DAOSWR010000013        23204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1408_length_23204_cov_5.702147, whole genome shotgun sequence.
ACCESSION   DAOSWR010000013 DAOSWR010000000
VERSION     DAOSWR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_23204_cov_5.702147"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK513_01925"
     CDS             <1..480
                     /locus_tag="PK513_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246603.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOO81242.1"
                     /translation="AMLIYRLPLNEVVFDFYDRLKSISKGYASFDYDLDGYGQNNLVK
                     LEIRVNEEPVDALAMICHRDNAESRGRQMCERLKELIPRQMFKIAIQAAIGGKIIARE
                     NVSALRKDVTAKCYGGDITRKKKLLEKQKKGKAKMRQYGNVEIPQSAFIAALKMGDE"
     gene            480..887
                     /locus_tag="PK513_01930"
     CDS             480..887
                     /locus_tag="PK513_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOO81243.1"
                     /translation="MSERWKQRFENFEKAYSVLADALDANFSDLSDLEKMGCIQAFEI
                     TFELSWKVMKDYLVYSGINLIEISPRKVIQEAMAAGLIQDGDMWMSMLKNRNLTSHTY
                     DESHILSGVEMIERDYLPHITALYNYLKGRHDG"
     gene            877..1230
                     /locus_tag="PK513_01935"
     CDS             877..1230
                     /locus_tag="PK513_01935"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO81244.1"
                     /translation="MMVEPNRYEDQPFGLPPLTIDNIRRFLMGWPEIERIEIFGSRAM
                     GNYRYNSDIDICVWGEAIDPDLFSRIRSGLQDLPIPYKIDVVQYETISHPPFKAHIDE
                     HGVDFFKALKMGRGE"
     gene            1230..1583
                     /locus_tag="PK513_01940"
     CDS             1230..1583
                     /locus_tag="PK513_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81245.1"
                     /translation="MLDFISENSQFLSVLTVTFAGLFAFIKWLDTRNRELKEKRYSKY
                     MQLISVISGKREDSSPSNLPEQIAATWFLIEYKEYFEITKKIFSNSDLKEMADETWIK
                     HVLPQMQSMLKEISK"
     gene            1834..2460
                     /locus_tag="PK513_01945"
     CDS             1834..2460
                     /locus_tag="PK513_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009572036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81246.1"
                     /translation="MTNLAHKIRDKIRELQSEVETELSEKRDEFQYRLERRKVIFEQE
                     ALALQREAKMGLIRYLRESRLLVVLTSPLIYSMILPIMICDLFVSVYQAICFPIYGIP
                     KVKRREYVVMDRKYLAYLNIIEKMNCIYCEYANGVIAYVREIASRTEQYWCPIKHARK
                     LKDPPARYYGYLEYGDSEALHKKWEAQRDKCRACEVPGKCTGKTEGER"
     gene            2457..2636
                     /locus_tag="PK513_01950"
     CDS             2457..2636
                     /locus_tag="PK513_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81247.1"
                     /translation="MKRRLVIAGIVLAVLVAGAFIYDAVTAKPQREILIDKDYRPAPP
                     KTYAPVDETLTPMPK"
     gene            complement(2671..3051)
                     /locus_tag="PK513_01955"
     CDS             complement(2671..3051)
                     /locus_tag="PK513_01955"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOO81248.1"
                     /translation="MFKQIEGLPENVLGIEVSGKLTHEDYVDGMLLLCDEMLEKHKPL
                     KMMLVIARDFGGMELSAMWDDMSYGIRHWNDLSHIAFVTDEGWIRNMTALFKPFFPGE
                     VKFYGLDEVEQAKDWIVGARELAA"
     gene            3245..3742
                     /locus_tag="PK513_01960"
     CDS             3245..3742
                     /locus_tag="PK513_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOO81249.1"
                     /translation="MIKYVSGDILLSDAQAIAHGIAPGDHFNQGLALTLRERFPAMAK
                     DFRHYCHQSNPPAGGAWLWAGPDGVIINLMTQDPAPDHNAHPGKASVKNISHALKELC
                     AIIDAESLSSVALPRLATGVGGQDWEDVKPLIEEHLGGLKIPVIVYEAYTKDEKAKEN
                     IPKAA"
     gene            3823..4518
                     /locus_tag="PK513_01965"
     CDS             3823..4518
                     /locus_tag="PK513_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOO81250.1"
                     /translation="MRVILILALAAVFSFPQTVAAQCGASPAPDLPDGHLMMNFSATE
                     RRDVEQDLLVTTLSYSATNRDVRALQNEVNSTMKKALERVKREKEVEAQTGAYQVYET
                     SDPHTKERLWRGSQNITLKSKNADDLLALSGDLQDMKLATHGLSYTLDPETAASVKDS
                     LMEAALIQLQQRADRAAKALGKSSASLREVTIVNEDASYSNLQMYERSMVGTMRQWIW
                     LCRLPKPERQQSA"
     gene            4570..4965
                     /locus_tag="PK513_01970"
     CDS             4570..4965
                     /locus_tag="PK513_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOO81251.1"
                     /translation="MALLFLYGTLMRGFRNAAQLDLGHFLGPVRTAREECSMVACVDP
                     PYPFPGVIAGHSHIAGELYDAGTDLLRRLDAFELEGQEYMRETIALNNGESAQYYRLI
                     YPSRITVVDQHAQIDFNKSENCYRWVHVE"
     gene            4973..5803
                     /locus_tag="PK513_01975"
     CDS             4973..5803
                     /locus_tag="PK513_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO81252.1"
                     /translation="MITLCEAAMDVLSTPEAVKKAEKTRQYAQAWRAGDICNIGHADL
                     PARPARSERPQLLAPKDMPKRAKAGSPRSKIALLHALAHIELNAIDLAWDVMARGFDL
                     GGFTRPKDFYDDWVKVADDEAKHHLMLEERLNALGASYGDLPAHDGLWESSEKTAHDF
                     AARLAIVPMVLEARGLDVTPGMIAAMEKQGDTETAHMLQIIHDDEITHVRAGTRWFEA
                     WCAHHGKDVEETWQDLVRTYFNGQLKRPFNHPSREEAGMIRDWYEPLAEALNDLEDVA
                     "
     gene            5892..7001
                     /locus_tag="PK513_01980"
     CDS             5892..7001
                     /locus_tag="PK513_01980"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HOO81253.1"
                     /translation="MNDTPEHTDTPSPEPEAQEVSRSRDDDFSVMVERLIRDAEDMRL
                     SFMKRHRTRGNVTMTLGLISLTIGATGFGWFLFMEADILRAVGSMLLAVMIPALLQIW
                     NNGILKDYERSYKREFLPRLAQALGGFKFHPSRGIGRKIIARTGLIPAHDIYEAEDCF
                     LGHYKGVKVLFSEARLKTKKKYIAPVFDGVFVLLEIPNAAIEGHTILTADRNAYRMWR
                     SSRWGKLQDVPISTGNKSWDRFQILSDNPEGAKLFIGERLLKELAEAADIFDNADISA
                     SFFKRKFVFLMIPYKGDMFEASNIHIPVATKQHAMQCKREIEQILEIIDVFDLYQKAD
                     TPPAEVKNEAEKAENSQPDSSLSQEPPTDSGLETD"
     gene            complement(6998..7321)
                     /locus_tag="PK513_01985"
     CDS             complement(6998..7321)
                     /locus_tag="PK513_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81254.1"
                     /translation="MPREDRRIIFSQDEVYKAIYALSVQKQLPKPPPGAVTSVKEKDS
                     DTSQIVLTLENPADQSIEIREYARDFLAAALMMFCRGAGIPLPKSAKKSVLIHDGEII
                     LRAQI"
     gene            7474..8001
                     /locus_tag="PK513_01990"
     CDS             7474..8001
                     /locus_tag="PK513_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81255.1"
                     /translation="MSEKPKSDKNNDETAQGVFSAVDSVAQRLSTIEQFIARRFDEIS
                     MEINATSQQVDMAEEGIIKRFAEILEILKAVSYAGDGSTAANAGVELDAVVEMTEQAA
                     NRILDAADHLTSRIEEEKNSSTDEGRKQAFEEMGKDVEEIYMACSFQDITGQRIRKTL
                     ENLRIIEDRLGSALD"
     gene            8329..8865
                     /locus_tag="PK513_01995"
     CDS             8329..8865
                     /locus_tag="PK513_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81256.1"
                     /translation="MVNITALKAEFTQKTIEGRIRGLACRLYKLAADPQAPHQRGPLF
                     ISLDSAFKPAAEYDGMLGSMMLDSFLGTAFADAGNDNTSSGMFEIGGTQEMDFLGEAA
                     SEYLKDHEKQARGQGSFARTEHKSLCNQFSTMQECDSFAANHPVRLQIEEALAALSRT
                     LGVAKRNTHTAPILEFCT"
     gene            8957..9925
                     /locus_tag="PK513_02000"
     CDS             8957..9925
                     /locus_tag="PK513_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81257.1"
                     /translation="MQNFTSSLLREKFVIHDPKAGNKKESAIIALSNRFVVELETPKD
                     SLDEVFIVRGHNMHSVVRMAARIIREFEQLGPIARRQPPFDWEKAWGSIVNDYEYAYN
                     PQRWIAIYHGGKCIFSAGEYHPFLDMIEKCDHGNNQDYDYAIPMAEKMFEAAGKRLNI
                     DHDANVALSVHFERGEGRCGVILRGSNQTTTFSFVAKSKSSGHAINIPQCLGGAAAFL
                     EGVQMAFLMGMNTEKIRIGLIERFSKEEKQTREGKQRLGRLANEIANMEAAHEVRYRP
                     EKPEFYLLMAQAEELGQRILEPPEKAPEGNELDAPTDGNTPDGEEV"
     gene            10042..11436
                     /locus_tag="PK513_02005"
     CDS             10042..11436
                     /locus_tag="PK513_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO81258.1"
                     /translation="MSQTAYSESINIAFSQEAEHWEQNGLSDDFSKQFDTSATFWACA
                     EENPSNDANESTPYGYDKAEEGALLACAKNGDETAYNTLFFKYEPSMRRLLMRFQTAA
                     NTDDILQDVYMKGWSGLRNFRGESCFHTWIYTIARNASINFSKTNKTYDGTSYDDLFD
                     SDLYGLSLEAGSDVLAFWEDRETLQAFIAALEALPDDMKKTFRLRIIEMKSCEETAAA
                     LGILEGTVKSRIFKAKKHIEEECAARGIDLQLTKTMKKPKKKAPIAKTVSVDSKCSPR
                     YPKLSHDIIVGHIWSHLEKFGAFPHKRSGEVFDKTSENWCALDMAGQARCRGLSDGMT
                     LKQRICAAIQEEAICHARLKGEIPEKDSGALPNHPDFDWEMVDNFLKSAKGRYKLSLE
                     DVLADVDFSEALEIYERRIKQEAAQMPTPDAQHRGVSTLYPWKPKIYDGLTREYEEQA
                     ILAALAFPIPDIEP"
     gene            complement(11460..14111)
                     /gene="acnA"
                     /locus_tag="PK513_02010"
     CDS             complement(11460..14111)
                     /gene="acnA"
                     /locus_tag="PK513_02010"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOO81259.1"
                     /translation="MTRTGHDSLQTRKTLEVDGKSYDYFSLKAAADKIGDISRLPYTL
                     KVLLENLLRFEDGRSVTTEDVQALADWLKAKKSSHEIAYRPARVLMQDFTGVPAVVDL
                     AAMREAMKALGGDPQKINPLVPVDLVIDHSVMVDEFGTPSAFQANVEHEFERNGERYE
                     FLKWGQGAFKNFRVVPPGTGICHQVNLEYLAQTVWEDGGVIYPDTLVGTDSHTTMVNG
                     LSVLGWGVGGIEAEAAMLGQPISMLIPEVVGFKLTGKMKEGNTATDLVLRVVEMLREL
                     GVVGKFVEFYGDGLDHLTLADQATIANMAPEYGATCGFFPIDEETLNYLRFTGRDEHR
                     VKIVESYAKEQGMWRDAKTPDPEFTTTLHLDLADVVPSISGPKRPQDRIALTDSVKAF
                     AKVCPEEKSVPVKGADYTLDNGDVVIAAITSCTNTSNPAVMMAAGLVARKAREKGLTA
                     KPWVKTSLAPGSQVVTSYLDKAGLTEDLNALGFDTVGYGCTTCIGNSGPLPGPIGAAV
                     EAGDLNVTSVLSGNRNFEGRISPHVKSNYLASPPLVVAYAIAGSMKVDLYKEPLGQDS
                     AGNDVFMKDIWPSNQEVEELMRTCITREMFLDRYGDVFKGTKEWQAIGTAAASETYSW
                     KDSSTYIQNPPFFEGMSNQAGDVKDIKGARPLAILGDSVTTDHISPAGSFKPDHPAGA
                     YLVEHGVQPKDFNSYGSRRGNDRVMTRGTFANIRIKNEMVPGTEGGYTRYMPSGEEMS
                     IYDAAMKYRQEGAQLVVIGGKEYGTGSSRDWAAKGTRLLGVKAVITESFERIHRSNLI
                     GMGVLPLQFKGGVNRETLKLDGSETFDITGISGGLKPAQDVAMSVHYADGSSKDVTLL
                     CRLDTLDEVEYFENGGILHYVLRSLAA"
     gene            complement(14402..15352)
                     /locus_tag="PK513_02015"
     CDS             complement(14402..15352)
                     /locus_tag="PK513_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81260.1"
                     /translation="MSDETADKKSETSDTDKKSVPERVFKEYNLKRIQAESVKITAER
                     AIEIIPKLAENKTRIIPFNIAKIFSFNAPLLQNDAVRHCTIEAFKNFPGAASHVAGLL
                     SIEGEYLNLAALYSRESFVQTHEDDYIAALQDIGGKEAVQELATTIARWCPEKAIAAL
                     ETMDDENAPEAIFLSGKVDFEAGLGQHNADALGAMALLHKRAKAGEFRSISTDSVQNF
                     TRALVKSLLDAPSLLKALEGCRPYPSKGSYSSFDIDDREDIPVPDELIASFKAALKAV
                     DDQGMLSRKVSRVFSDVVKHHKRTQKSENSLQSRIEKMEL"
     gene            15477..16637
                     /gene="metB"
                     /locus_tag="PK513_02020"
     CDS             15477..16637
                     /gene="metB"
                     /locus_tag="PK513_02020"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOO81261.1"
                     /translation="MSIQKPLHKRRRTTAVRAGVGTDPAYRSVMPPLYLSSTFQIDGL
                     DKRGDYEYSRTRNPTRDELANALAALEGGIGCAVTASGMAALTLLLHLLGPDDLVLAP
                     YDMYGRSYWLLRALAEKKHFRLKFIDCYDEGALTQAFDKNPRMVILETPSNPVMRIAD
                     IADISKRAKACGALTVCDNTFLSPILQNPLKLGADIVFHSTTKFLNGHSDVVGGCVIV
                     KDDEQFLADLHLWNNSLGTIGAPFDAYMTLRGMRTLEVRVHRGEENAAAIAHYLQEHS
                     KVSKVFYPGLPGHEGHEIAKRQQEGFGAMLSFTLEGDMDGFLSKLEMFSLAQSLGGTE
                     SLINHPASMTHVSMGAEARAKAGVTDNLLRLSVGIEHIDDLLSDLEAALNVA"
     gene            complement(16723..17427)
                     /locus_tag="PK513_02025"
     CDS             complement(16723..17427)
                     /locus_tag="PK513_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81262.1"
                     /translation="MIPFDPVTAGTALILAVSSPQICQMTTSADISVVPKTAELVIDT
                     SRSAASLQGQSIDTINPYGYNNVTHTNGFMEGQINMRSSVELDYKLTSRANAYCIYYK
                     KVTITLDITPKIVIAKEVSEDSCMYGAVLEHEMKHVNADRKIVNKYAQTIGKKVFDGL
                     RQRGFIAGPIAAENAQTIMDRMKNTVAQLVQFEYKKMEIERTEAQQAIDNLAEYQFVQ
                     GQCPDYESPLAASRGQ"
     gene            17512..17790
                     /locus_tag="PK513_02030"
     CDS             17512..17790
                     /locus_tag="PK513_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOO81263.1"
                     /translation="MGMIAYLVSLAFDIYIGIIVIQVVVSWLIIFDVINAENAQAQNL
                     VALLKRATDPIYKPLQKYIPPIGGIDVTPIVVILGLSLLQNIVVGVLI"
     gene            17817..18665
                     /gene="folD"
                     /locus_tag="PK513_02035"
     CDS             17817..18665
                     /gene="folD"
                     /locus_tag="PK513_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOO81264.1"
                     /translation="MSETQNIDGKAVAKTLREELADEVAGLNIQPGLAVILVGEDPAS
                     QVYVHNKIKACEACGIKSFESRLPAQATEAEIMAEIEAFNDNKDVHGILLQLPLPYPL
                     NADVLIQHIKPEKDVDGLTFINQGKLIAGDKSGLVPCTPQGSMMLIKSVEEDLSGKHA
                     VVIGRSLLFGKPMAQLLLAENCTVTSAHSRTQNLPEICQQADILVAAVGRPKMVQGAW
                     IKPGAIVIDVGINRQDDGKLCGDVDYEQARGVASAITPVPGGVGPMTIACLLKNTVEA
                     AKKAKN"
     gene            18806..19633
                     /locus_tag="PK513_02040"
     CDS             18806..19633
                     /locus_tag="PK513_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81265.1"
                     /translation="MSKKALSERPLSQRFSDIATLVRGVRNNVPEFSEDSGFAVDPWD
                     KHKNVRPHVEAMLHISETIRHSQTYLDFLENSRDLTLRSIQDDRGLLESIVHYPVSCE
                     NDQKAVIQAVNLYQAAASCEASDFDFGETNIVFFEEAKRYYKQSNAIRILNGSSACNL
                     KTGSRKIKINTHDDAGFRNPLKALSIVFHEGVHDQIFQLAFACYTKKGNLGNLAGDAK
                     LYATFKEQGATIPFGIRLAYLEQFYEIVAHDQQDKFARDLNNLLKSADSGFTLSHPH"
     gene            complement(19630..20343)
                     /locus_tag="PK513_02045"
     CDS             complement(19630..20343)
                     /locus_tag="PK513_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81266.1"
                     /translation="MTPASQVFAQNGPAYRSFQKATPQTLEEAMRAPRVPGMPQQLKF
                     PYKQPLEPVFDKVRFIKQEPPSTLRERIERLIHGIYVDIPPEYDLYGYEIRRYMAHIG
                     GQAVLNSSQNIQGQLKNIKTAEIILRYWHKEIDKEASAIEAEIEETKASPSIRSSFKY
                     HRGVAQAFFVEASSWLGNNRKLLEYLDGIGVDAYEFKDPDMTFKNFMQLQQYAIHLKA
                     RNDALEQIQGYTPFLRMVY"
     gene            complement(20440..21882)
                     /locus_tag="PK513_02050"
     CDS             complement(20440..21882)
                     /locus_tag="PK513_02050"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOO81267.1"
                     /translation="MTKTPITVARGDGIGPEIMDATLQILEAAGAQLEIEEIEIGEAV
                     YKSGIKNGIAEESWESLRRTKVFLKAPITTPQGGGFKSLNVTVRKSLGLFANVRPCVS
                     YHPFVRTNHPKMDLVIIRENEEDLYGGIEYRQSRDVMASLKVISRPGTERIMRYAFEY
                     ARANNRKKVTCMSKDNIMKISDGLFHQVFDEIAAEYPDIENEHMIIDIGTAKVANRPD
                     LFDVIVTTNLYGDIISDVAAEVTGSVGLGGSSNIGLDFGMFEAVHGSAPDIAGQGIAN
                     PSGLILGSVMMLTHIGQGSVAAKIHNAWLRTIENGVHTGEIYRKGISKEQVGTEGFTK
                     AVIERLGQEPEVLPVKSYPDVAGGLKLPPLKPSVEVSKERVGVDIGINWDGSADDLAA
                     AINACVNDNLKLQMISNRGTKVWPDGQPETFCADNWRLRFMSDNGETVKTSQVIALMD
                     RMNKADLNFTKSVMLHNYDGKPGFTVAQGE"
     gene            22112..22624
                     /locus_tag="PK513_02055"
     CDS             22112..22624
                     /locus_tag="PK513_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO81268.1"
                     /translation="MPCNDAMIEDVVTARPNQTVSEVLELFSKYNIRSVPIVDENRSV
                     VGLFSFSHLLYSLLPVPATLGDHMMRLKHMDISLDHLLGASPWVAKRLNIILPKTMEE
                     VMLKNPVCVRVDSPIREGIRLMVKYDSPLPVVEDEKNRKLVGLISSQSVLGVLTEIAS
                     HLEKGKEVHE"
     gene            22684..>23204
                     /locus_tag="PK513_02060"
     CDS             22684..>23204
                     /locus_tag="PK513_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO81269.1"
                     /translation="MHELVFDTPMLISAIILIVTFAGIFTEGLHHIHRTKIAMLGAAA
                     MVIIGQVFGFYNPEKAIEAIDWNVVFLLGAMMTIVAIMIPTGGFQAIAYWIADFSKGR
                     LFLLLALMGTAVTVISLLLDNVTTVVIFGPLIVLICQALKVNPIPYLLAAALLSDTGG
                     VATLVGDPPNLMIG"
BASE COUNT         5923 a         5260 c         5992 g         6029 t
ORIGIN      
        1 gcgatgctga tctaccgtct gccgctcaac gaggtggtgt ttgactttta cgaccggctg
       61 aagagtattt ccaaaggcta tgccagtttt gattatgatc tggacgggta tgggcagaac
      121 aaccttgtga agttggaaat ccgggtgaat gaagagccgg tggatgcgct ggcgatgatt
      181 tgccaccgcg ataatgcgga atcgcgtggt cgccagatgt gcgagcggtt gaaagagtta
      241 atcccgcgcc agatgttcaa aatcgccatt caggcggcaa tcggcggcaa gattatcgcg
      301 cgggaaaatg tctccgcgct gcgcaaggac gtgaccgcga aatgttacgg cggggacatt
      361 acgcgcaaga agaagctgct ggaaaagcag aaaaaaggca aagccaagat gcgccaatat
      421 ggtaatgtag aaattccaca aagcgcgttt atcgccgcgc tgaaaatggg ggatgagtga
      481 tgagcgagcg ttggaagcaa cgatttgaaa actttgagaa ggcttatagc gtattggctg
      541 atgcgcttga tgctaatttc agtgacctgt ccgatctgga gaagatggga tgcattcagg
      601 cgtttgagat tacgttcgag ctatcgtgga aagtgatgaa agattatctg gtttactcag
      661 gaatcaattt gatcgagatt agcccgcgca aagtgattca ggaagcgatg gcagcagggc
      721 ttattcagga cggtgatatg tggatgagca tgcttaaaaa ccgcaatctt acatcacaca
      781 cctatgatga atcgcatatt ttgagcggtg ttgagatgat tgaaagagat tacttaccgc
      841 atattacggc gctttataac tatcttaaag ggcggcatga tggttgagcc gaaccgttat
      901 gaagaccagc ctttcggcct gccgccgctg acgattgaca atatccgccg gtttcttatg
      961 ggctggccgg agattgagcg catagagatt ttcggatcgc gcgcgatggg gaattaccgt
     1021 tataactccg atattgatat ctgcgtctgg ggcgaagcta tagatcccga cctgtttagc
     1081 cgtatccgct ccgggctgca agatctaccg atcccttata agattgacgt ggtgcagtat
     1141 gaaaccatct cccatccgcc cttcaaagcg catatcgacg aacacggcgt tgattttttt
     1201 aaggcgctca agatggggag gggggagtga tgttagattt tatttctgaa aacagccaat
     1261 ttttaagcgt ccttacagtt acttttgcgg gacttttcgc ttttataaaa tggttagaca
     1321 ccagaaatag agaactcaaa gaaaaacgtt actcaaagta catgcagttg attagcgtaa
     1381 tttcaggaaa aagagaagat agttctccct ctaatttacc agagcagatt gccgctacgt
     1441 ggtttttgat cgaatacaaa gaatacttcg aaataacaaa gaaaatattt tctaacagtg
     1501 acttaaaaga aatggccgat gaaacatgga ttaagcatgt cctgcctcag atgcaaagca
     1561 tgttaaaaga aatatcaaaa taaatctttg gaaccgtccc ctgcccaaaa atatcattcc
     1621 ttttcgtcat tgcgaggagc cgcaggcgac gcggcaatcc agccttttga accacgaata
     1681 aacacaaata cacacagatg agattgcgag agaaataatc tgtgttcatc ggtgtttatc
     1741 tgtggttaat gtttctggat tgcttcgctg cgctcgcaat gacatgatat tgtttcagca
     1801 cagccaaaaa gataagggaa tgaattaacc cacatgacca atctcgcgca taaaattcgt
     1861 gacaagatcc gtgagctgca aagtgaggtt gaaaccgagc tgtccgagaa gcgtgatgag
     1921 ttccaatacc ggttggaacg ccgtaaggtg attttcgagc aagaggcgct ggccctgcag
     1981 cgcgaggcca aaatgggcct gatccgatac cttcgggaat cacggttgct ggtcgtgctg
     2041 acctcgccgc tcatctacag tatgatactt ccgatcatga tttgcgatct gtttgtcagc
     2101 gtttatcaag cgatctgttt tccgatttac ggcattccca aggtcaagcg ccgcgagtat
     2161 gtggtgatgg atcgcaaata tctagcctat ctcaacatca ttgagaagat gaactgcatt
     2221 tactgtgaat atgccaacgg ggtgattgcc tatgtgcgcg agatcgccag ccggacggag
     2281 caatattggt gcccgattaa acacgccaga aagctcaagg acccgccagc gcgttattac
     2341 ggttatcttg agtatggcga cagcgaggct ttacacaaga agtgggaggc acagcgcgat
     2401 aagtgccggg cgtgtgaagt gccaggtaag tgcaccggga agacggaagg tgagcgatga
     2461 aacgtagatt ggtgattgcg gggattgtgt tggcggtgct ggtggccggg gcgtttatat
     2521 atgacgccgt caccgcaaag ccgcagcgcg aaattctgat tgataaggat taccgccctg
     2581 ctccgccgaa gacctatgcg cccgtagatg agacgctgac gccgatgccg aagtaaacgt
     2641 cgttgcactc ctcgcaatga cgatggcgtc ctaggctgcc agttcccggg cgccgacgat
     2701 ccagtctttc gcctgttcga cctcgtccag cccatagaat tttacttcac cagggaagaa
     2761 gggcttaaac agcgcggtca tattgcgaat ccagccctcg tctgtgacaa aagcgatatg
     2821 cgaaagatca ttccaatgtc tgatcccgta gctcatatca tcccacatgg cggaaagttc
     2881 catgccgccg aaatccctag cgatcacaag catcattttc aacggcttgt gtttttccag
     2941 catctcgtcg cacagcagca acatgccgtc aacgtagtcc tcgtgcgtca atttgccact
     3001 cacctctata cccagtacgt tttcgggaag gccttctatt tgtttaaaca ttgtctttac
     3061 tcctttttgc atggatatgg tcccagcgta tgcctgtttg ctggcctgcg atttgacatt
     3121 tatcaagcgc ggcaaaatag acaccaaccc cccttcccca ttattaagaa atcgcataga
     3181 atggagttcg tcaggcgcag ttttgcgctc tttcaacgcg ctctttcaaa acaaggacaa
     3241 atccatgatt aagtatgttt ccggtgatat tcttcttagc gatgcgcaag ccattgcaca
     3301 cggcattgcg ccgggcgatc attttaatca gggattggcg ttgacgctgc gtgagcgttt
     3361 tccggcgatg gcaaaggatt ttcgtcatta ttgtcatcaa agcaatccac cggccggtgg
     3421 ggcgtggctg tgggccgggc cggacggggt gattattaat ttgatgacgc aggaccctgc
     3481 gccggatcat aatgcgcatc caggcaaggc cagtgttaaa aatatctctc atgctttgaa
     3541 agaactttgc gcgattatcg atgcggagag tctgagttcc gttgctctgc cgcggctggc
     3601 aaccggtgtc ggcggacagg actgggagga tgtcaagccg ttgatcgaag agcatctggg
     3661 cgggcttaag attcctgtga tcgtttatga ggcctatacc aaggatgaaa aagcaaaaga
     3721 gaatattccc aaagctgcat aggggtccgc tcacttttca aaagattgct cttccttttt
     3781 caaatcgcta tgctggcttt tccagcaatc aggaggactt tcatgcgtgt aattttgata
     3841 ctggccttgg ccgctgtttt ctcattcccc caaacggtgg cggcgcaatg cggcgccagt
     3901 cctgcgcccg atctgccgga cgggcatttg atgatgaatt tctcggcgac cgagcggcgc
     3961 gatgttgagc aggatctgct ggtgacgacg ctgtcttata gtgcaaccaa ccgtgatgta
     4021 cgcgcattgc aaaacgaagt taatagcacc atgaaaaaag cacttgagcg cgtcaaacga
     4081 gaaaaggaag ttgaagcgca aacaggagcg tatcaggtgt atgaaaccag tgatccacat
     4141 acgaaagaac ggctttggcg cggatctcag aatattacgc tcaaatccaa aaatgcggat
     4201 gacttgctgg ctcttagcgg agatttgcag gatatgaagc tggcaacgca tgggcttagc
     4261 tatacgcttg accctgaaac ggctgcaagc gttaaggata gtttgatgga agcagcgctg
     4321 atacagctcc aacagcgagc ggatcgcgcg gcgaaggcgc ttggaaagtc cagcgcttct
     4381 ttgcgtgagg ttacaattgt gaatgaggac gcctcctact ctaacctaca aatgtatgaa
     4441 cgcagtatgg tagggacgat gcggcaatgg atatggctgt gccggttgcc caagccggag
     4501 agacaacaat cagcctgagc gtgagtgcaa ctgcggtttt gaaaccttaa ttaagagcaa
     4561 tatgaatcca tggccttgct gtttctttac ggcacattaa tgcgggggtt tcgcaatgcc
     4621 gcgcagcttg atctcgggca ttttttaggc ccggtgcgta cggccagaga ggagtgcagc
     4681 atggtggcat gtgttgatcc tccttatccg tttccgggcg ttattgccgg acacagccat
     4741 atcgccgggg agttatatga tgcgggcacc gatctgttac gtcggcttga tgcttttgag
     4801 cttgaaggtc aggaatacat gcgtgaaacc atagccctaa acaacggtga aagtgcgcag
     4861 tattacaggc ttatttatcc aagcaggatt acggttgttg accagcatgc acagattgat
     4921 tttaacaaaa gcgaaaattg ctatcgctgg gttcacgtag agtaaaaaag tgatgataac
     4981 attatgcgaa gcggcaatgg atgtgttgag cacgcctgaa gcggttaaaa aagcagaaaa
     5041 gacaaggcaa tatgcacagg cgtggcgcgc tggcgatatt tgcaatatcg ggcacgctga
     5101 tctgcctgcc agaccggcgc gcagtgaaag gccgcagctt cttgctccca aagacatgcc
     5161 taaacgtgcg aaagccggaa gcccgcgctc caaaatcgct ctactccatg cgctggcgca
     5221 tattgagctt aatgcgattg atctggcatg ggatgtgatg gcgcgggggt ttgatttagg
     5281 cggctttacg cgccccaaag acttttacga tgattgggtt aaggtggcag atgacgaagc
     5341 caagcatcat ttgatgctgg aggagcgttt aaatgcgctg ggcgcttctt atggcgacct
     5401 gcctgcccat gacggtttgt gggaatcctc ggagaaaacg gcgcatgatt ttgcggcgcg
     5461 ccttgcgatt gtgcctatgg ttctggaagc gcgtggactg gacgtgacgc cgggaatgat
     5521 tgccgctatg gagaaacagg gcgacaccga aacggcccac atgctgcaaa ttattcatga
     5581 tgacgagatt acgcatgtgc gcgcaggaac gcgctggttt gaggcatggt gcgcgcatca
     5641 tggaaaagac gttgaggaaa cgtggcagga tttggttcgc acctatttca acgggcagct
     5701 taaacgccca tttaatcacc cttctcgcga agaggctggg atgatccgcg attggtatga
     5761 accgctggcc gaggcgttga atgatctcga agacgtggct tagactcagg acctaaaaga
     5821 caaaaggaat tcaagcactc tttggcaaag atttcttgtc cataatgaca atagcgtgtt
     5881 taatttagcc tatgaatgac actccagagc atacagatac gccatcaccg gagcctgaag
     5941 cgcaggaggt ttcgcgcagc cgggatgacg atttttccgt gatggttgaa cggttaatcc
     6001 gcgatgcgga agatatgcgg ctatcgttta tgaagcgtca ccgcacacgc gggaacgtga
     6061 ccatgacatt ggggcttatc agcctgacaa ttggcgcgac cggatttggt tggtttttgt
     6121 ttatggaagc cgacatcctg cgcgctgttg gctctatgtt gcttgcggtt atgatcccgg
     6181 ccttactgca aatctggaac aacgggattt tgaaagacta cgagcgcagc tataaacgag
     6241 agtttttgcc gcgccttgcc caggctctgg gcgggtttaa atttcatcct tcacgcggga
     6301 tcgggcgtaa aattatcgcc agaaccgggt tgatcccggc tcatgacatt tatgaagctg
     6361 aagattgttt tttggggcat tataagggcg ttaaggtgtt gttttccgaa gcgcgcctta
     6421 aaaccaagaa aaaatatata gcgcctgttt ttgatggcgt gtttgtattg ctggaaattc
     6481 ctaatgcagc aatcgagggg catacaattc taacggctga tcggaacgcc tatcgcatgt
     6541 ggcgatcatc acgctgggga aagcttcaag acgttccgat ttcgaccggc aacaaaagct
     6601 gggatcgttt tcaaattctt tccgataatc ctgagggcgc aaagcttttt atcggtgagc
     6661 ggctgctcaa ggaacttgca gaagcggctg atatttttga caatgcggat attagcgcgt
     6721 cgtttttcaa aaggaaattt gtattcctta tgattccata taagggcgat atgtttgaag
     6781 cctccaacat tcatattccg gttgcgacga aacagcatgc gatgcaatgc aaacgcgaga
     6841 tcgagcagat tttagagatt attgatgtgt ttgatctgta tcaaaaagcg gatacgccgc
     6901 ctgccgaggt taaaaacgag gctgaaaaag cggaaaactc gcagcctgat tcatccctct
     6961 cacaagaacc tccaacggat tcaggtttgg aaacagatta aatctgcgcc cgcaggatga
     7021 tttcgccatc gtggatgagc actgattttt ttgcgctttt gggcaacggg atccctgctc
     7081 cccggcaaaa catcataagc gcggcggcca gaaaatcccg ggcatattca cgaatttcta
     7141 tactttgatc tgctggattt tcaagtgtca agacaatctg cgatgtgtca gaatcttttt
     7201 cttttacact ggtcactgca cccggtgggg gttttggcaa ctgtttttgt acactcaggg
     7261 cgtatatagc cttgtaaact tcatcctgac taaaaataat tctacgatct tcacggggca
     7321 taagggaaaa atcaacctcg gcgtcttttt aaatacgccc gtataataat aagaaagtaa
     7381 taggcaggat agaagaattt tattaatatc tgcatgatat agtttattag tccttcaacg
     7441 ctaatcactt ttcagcagaa agcagtggaa gtcgtgagcg aaaaaccaaa atcagataaa
     7501 aataacgatg aaacggcgca aggcgttttc agcgcagttg acagtgttgc gcaacgactc
     7561 tcaaccattg aacagtttat cgcccggcgc tttgatgaga tttcgatgga aattaatgcg
     7621 acatctcagc aagtcgatat ggctgaagaa ggtattatca agcggtttgc cgagatttta
     7681 gaaattctca aggccgtttc ctatgccgga gacggctcaa cagcggccaa tgccggggta
     7741 gagctggacg cggttgtgga aatgaccgaa caagcagcta accggatttt ggatgcggcg
     7801 gatcatttaa ccagccggat tgaagaggaa aaaaactctt ctacagatga agggcgcaag
     7861 caagcctttg aagagatggg aaaagacgtc gaagaaattt atatggcatg ttcgtttcag
     7921 gatattaccg gccagcgtat ccgtaaaacg ctggagaatt tgcgtattat cgaagatcgt
     7981 ctcggcagcg cacttgacta aatgggcatt caaatagatg taaacccaga agatcatgtt
     8041 gcgattaaaa acaagctttc tacgcaaaac gatattgata atttcttcca aacaaaaaaa
     8101 ggtgagaatt agcttaaggg catagctctg cgctttttta tacttactcc tttatacctg
     8161 ctcccgccgc atctgttttg cggcggtttt tatataccga aagtaaatat ataaaatttt
     8221 atataaatac agtttaagta atatttgtaa ctattactta aagttttatc cataattcag
     8281 caaatattta cttcgtcttc gtatcctcta agaaaaaaga gggtcacgat ggtgaatata
     8341 actgccttaa aagcagaatt tacacaaaaa acaatagagg gacggatcag gggacttgca
     8401 tgcaggcttt ataaactggc agcagatcca caagctccgc accagcgtgg cccacttttt
     8461 atttccctgg atagcgcatt taaacctgcc gctgagtatg acggcatgct tggcagcatg
     8521 atgctggata gttttttggg cacggcattt gccgatgccg gcaacgataa tacctcctcc
     8581 ggaatgtttg aaattggcgg tacacaggaa atggattttt taggtgaagc ggccagtgaa
     8641 tatttaaaag atcatgaaaa acaagcccgc ggtcagggca gctttgcacg cacagagcat
     8701 aagagtctgt gcaatcaatt ttcaacgatg caagaatgcg acagctttgc ggctaatcac
     8761 ccggtccgcc tgcagattga agaggcgctg gcggcgctca gccgaacatt aggcgtagca
     8821 aaacgcaaca cgcacaccgc gcccatatta gagttttgca cttaaaacac ctttgaattg
     8881 aaaagcttgg ccccttcttc tcattaagat atgatgaatt tggatttgta gattcagccc
     8941 cacgcaacga gctcttatgc aaaattttac ttcatcatta ctgcgcgaaa aatttgttat
     9001 tcacgaccct aaggccggaa acaaaaaaga gagtgcgatt atcgcgctga gcaatcgttt
     9061 tgtcgttgag cttgaaaccc ccaaagacag tttggatgag gtctttattg tccgggggca
     9121 caacatgcat tccgtggtgc gtatggcggc gcgcattatt cgtgagtttg agcaattggg
     9181 gccaattgcc cggcgtcaac cgccttttga ttgggaaaaa gcgtggggct ctatcgtcaa
     9241 tgattatgaa tatgcttata atccacagcg ctggattgcg atttaccatg gcggaaaatg
     9301 cattttttct gccggagagt atcacccgtt tctggacatg attgaaaaat gcgaccatgg
     9361 caacaatcaa gattacgatt acgctattcc gatggctgaa aagatgtttg aggctgcggg
     9421 aaaacgtttg aatattgatc atgatgccaa tgtggcgtta tctgtacatt ttgagagagg
     9481 tgaagggcga tgcggcgtga ttttgcgcgg ttcaaatcag acaacgacct ttagttttgt
     9541 tgcaaagtca aaatcatccg gccatgccat taacattcca cagtgcctgg gcggcgcggc
     9601 ggcgtttttg gaaggtgtac aaatggcctt tctgatggga atgaatacag agaaaatccg
     9661 tatcggtctg attgaacgat tttccaagga agagaagcaa acccgtgagg gcaagcagcg
     9721 gctgggccgg ctagccaatg aaatcgccaa catggaggca gcgcacgaag tgcggtatcg
     9781 ccctgaaaag ccagagtttt atttgctcat ggcgcaggct gaagagctgg gacaacggat
     9841 tttggagccg cctgagaaag cgccagaagg taatgagctt gatgccccta ctgatggaaa
     9901 cactccagat ggtgaagagg tttaaaacat ccttattcca ttgtattttt aggtatatta
     9961 gaaaaactta ttgttttttg cttatgatta tgtaattgtt gtggattcgt ttatttagga
    10021 tgctgacgtc tttatattgc catgagtcaa actgcgtatt ctgagtccat aaacatcgct
    10081 ttttcccaag aggctgagca ttgggaacag aatggtctgt cagacgattt ttcgaagcaa
    10141 tttgatacgt cggcgacatt ttgggcctgc gccgaggaaa acccctctaa cgatgcaaat
    10201 gaaagcacac cttatggtta tgacaaagca gaagaaggtg ccctattagc ctgcgcaaaa
    10261 aacggagacg agaccgccta caatacgctt ttcttcaaat atgagccaag catgaggcgg
    10321 cttcttatgc gttttcaaac agctgcgaac acggatgaca ttttgcaaga tgtttatatg
    10381 aaaggctgga gtggcctcag aaattttaga ggagaaagct gctttcatac atggatatac
    10441 acgatagccc gtaacgcgag cataaatttt tcaaaaacca ataaaacata cgatggcact
    10501 agctatgatg atctttttga cagtgatctg tatggcctgt ctcttgaggc tggcagtgat
    10561 gttttagctt tttgggaaga cagggaaact ctgcaggctt tcattgcggc tcttgaggca
    10621 ctgcctgacg atatgaaaaa aactttccgc ttaagaatta ttgagatgaa atcctgtgaa
    10681 gaaacagccg ccgctctcgg tattttagaa ggaaccgtta aatcaagaat tttcaaagct
    10741 aagaaacata tagaggagga atgcgcggcg cgcgggatag atttacaact taccaaaaca
    10801 atgaaaaagc cgaaaaagaa agcccctatc gccaagaccg tttctgtcga tagtaaatgc
    10861 agcccacgat atcccaaact cagccacgat attattgttg gccatatttg gtcgcatctt
    10921 gaaaaatttg gtgcatttcc tcacaaacgt tccggtgaag tttttgataa aaccagtgaa
    10981 aactggtgtg cccttgatat ggcaggacag gcgaggtgcc ggggcttaag cgatggtatg
    11041 acactcaaac agcgcatatg cgctgccata caagaagaag caatatgcca cgcgagatta
    11101 aaaggcgaaa ttcctgaaaa agacagcgga gccttgccaa atcatcccga ttttgattgg
    11161 gaaatggttg ataattttct taaaagtgcc aagggccgtt ataaactttc actcgaagac
    11221 gtgctggctg atgtggattt tagtgaggct ttggaaatat atgagcggcg catcaagcaa
    11281 gaggcagcgc agatgccaac gccggatgcg cagcatcggg gcgtgagcac actctatcca
    11341 tggaaaccca aaatatatga tggactcacg cgtgaatacg aagaacaggc tatcctggcg
    11401 gctcttgcgt ttcctatccc cgatatagag ccttaaaacc ctgccaagcg ggctttagat
    11461 tacgcggcca ggctgcgcaa gacataatgc aaaataccgc cattttcaaa atattcgact
    11521 tcatcgagcg tatcgagacg acagagcagt gttacgtctt tgcttgaacc atcggcgtaa
    11581 tgcacgctca tggcgacatc ctgggcgggt ttgagaccgc cggaaatgcc agtgatgtca
    11641 aaagtttcgc tgccgtccag tttcaaggtc tcgcggttta caccgccctt gaattgcagc
    11701 ggcaggacgc ccatgccgat aaggttggag cggtgaatac gctcgaaact ttcggtgatg
    11761 acagctttga cgccgagcaa acgcgtgccc ttggccgccc agtctcgcga tgagcctgtg
    11821 ccgtattcct tgccgccaat cacaacgagc tgcgcacctt cctggcggta tttcatggcc
    11881 gcatcataga tactcatctc ttcgcctgat ggcatatatc tggtatagcc gccttcggta
    11941 ccgggaacca tttcgttttt aatgcggata ttggcgaaag tgccgcgagt catcacacgg
    12001 tcgttgcccc ggcgcgagcc gtaggagttg aagtctttgg gctggacgcc atgttcgacc
    12061 agataggcac cggcagggtg gtcgggttta aatgatccgg cgggagagat atggtccgtg
    12121 gtcacagaat cgccaagaat ggctaatgga cgtgcgcctt taatgtcctt cacatctcca
    12181 gcctggttgc tcatgccttc gaagaatggc gggttttgaa tataggtaga gctgtctttc
    12241 cagctatagg tttcactggc agccgctgtg ccgatggcct gccattcttt tgtgccttta
    12301 aagacatcac catagcggtc aaggaacatt tcacgcgtaa tgcaggtgcg catcagttct
    12361 tcaacctcct gattacttgg ccagatgtct ttcataaaca catcgttgcc ggcggagtcc
    12421 tggcctaatg gctctttgta cagatcgacc ttcatggacc ctgcaatggc gtaggccaca
    12481 accaatggcg gggaggccag atagttggat tttacatgcg gtgaaatgcg cccctcaaag
    12541 ttgcggttac cagacaggac ggaggttacg ttcagatccc ctgcttccac cgctgcgcca
    12601 atggggccgg gcaaggggcc ggaattgccg atacaggtgg tgcagccata gccgaccgta
    12661 tcgaagccga gggcgttgag gtcttcggtc aagcccgcct tatcaagata gctggtcaca
    12721 acctgtgagc cgggcgcaag ggatgttttt acccatggct tggcggtgag gcccttttcg
    12781 cgggctttgc gcgctacgag gcccgcggcc atcattacgg ccgggttgga cgtgtttgtg
    12841 caagaggtaa tcgcggcaat cacaacatcg ccgttatcca gcgtatagtc cgcacctttg
    12901 acaggaacag atttttcttc agggcagact ttggcgaatg cttttacaga gtcagtcaga
    12961 gctatgcggt cttgcgggcg tttcgggcca gagatagagg gaaccacgtc ggccaaatcc
    13021 agatgcagcg tggtggtaaa ttcaggatca ggtgtctttg cgtcgcgcca catgccttgt
    13081 tcttttgcat agctttccac gatttttacg cggtgttcgt cgcggcccgt gaagcgcagg
    13141 tagttgagcg tttcttcatc aatcgggaag aagccgcagg ttgcgccata ttccggagcc
    13201 atgttggcaa tcgtggcctg atcggccagt gtgagatgat cgagaccatc accgtagaat
    13261 tcaacgaatt taccaacaac gcccagctcg cgaagcattt caaccacgcg caagaccaga
    13321 tcggtggcgg tgttgccttc tttcattttg ccggtcagct taaagccaac gacttcaggg
    13381 atgagcatag aaattggctg gccaagcatg gcagcttcgg cctcgatacc gccgacgccc
    13441 cagcccagaa cagataatcc gttgaccatt gtggtatgag aatctgtgcc gaccagcgta
    13501 tcaggataga ttacgccgcc gtcttcccag acagtctggg caagatattc aagattgacc
    13561 tgatggcaga taccggtgcc cggcgggaca acacggaaat ttttaaaggc tccctgcccc
    13621 catttgagga actcatagcg ctcaccgttg cgctcaaact cgtgttcaac attggcttgg
    13681 aaagcagatg gagtgccaaa ttcatcaacc ataacggagt gatcaatcac gagatcgacg
    13741 ggcaccagcg ggttgatttt ctgtggatca ccaccaaggg ctttcattgc ctcacgcatt
    13801 gcggccagat caaccaccgc agggacgccg gtgaaatcct gcatcaatac gcgcgcgggg
    13861 cggtaggcga tttcatggct tgattttttg gccttgagcc aatcggccag cgcctgcaca
    13921 tcttctgttg ttacagaacg gccatcttca aaccggagca ggttttcgag caggactttg
    13981 agggtataag gcaggcgcga tatgtcgcca atcttgtctg cggcggcttt aaggctgaaa
    14041 taatcgtagc tttttccatc aacttcgagt gttttacgtg tttgcagaga atcgtggccg
    14101 gtgcgtgtca tttttttatc ccttgaaatt tgagcctgaa ttagaccgtt gcaagagcat
    14161 atcatgagtt gaaatggttt aaaatctgta aaaatacgtg agaattcaat gtgttatgaa
    14221 aatccttaaa atgagcatag tctgcccttt tttttgccac atttggaaga gatactttaa
    14281 agtatagaca aaccagatcg gaattagaag aattcagacc tctactgtct aaattagctc
    14341 actaccgtaa ttttaccgcc gggccttacc accccggcgg ttttacgttt tgggcagact
    14401 tttaaagctc catcttttca attcggcttt gcagactgtt ctcagacttt tgcgttcttt
    14461 tatggtgttt gacaacgtca ctaaaaacgc gggatacttt tcgggaaagc attccctgat
    14521 catctaccgc ttttagcgcg gccttaaaac ttgcaattaa ttcgtccgga acagggatat
    14581 cttcacgatc atcaatgtca aaactgctat atgatccttt tgaaggatac ggtcggcaac
    14641 cctcaagcgc cttcagaagc gacggtgcat caagaaggct tttgaccagc gcccgtgtaa
    14701 agttttgaac gctatcggtg ctaatgctcc taaattctcc ggcttttgct cttttgtgga
    14761 gcaaggccat agcgccgagc gcatccgcat tatgctgacc aaggcccgct tcaaaatcga
    14821 ctttaccgct caggaagatc gcttctggcg cattttcatc atccatggtt tcaagagcgg
    14881 caatagcttt ttccgggcac catctggcaa tagtggtcgc caattcctgt acagcctctt
    14941 ttccgccaat gtcctgcaag gctgcaatat aatcatcctc gtgcgtttga acgaagctct
    15001 ccctggaata aagcgcagcc aggttcaaat attcaccttc aatactcagt aaacctgcca
    15061 cgtgagacgc cgcgcctgga aaatttttga acgcttcaat cgtgcaatga cgcacggcat
    15121 cattttgcaa aagcggcgca ttaaaactaa atatttttgc aatattaaac ggtattatac
    15181 gcgttttatt ttcagcgagt tttggaatta tctcaatagc cctttcggcc gttatcttga
    15241 cggattctgc ctggatacgt ttcaaattat actctttaaa cactctttcc ggcaccgatt
    15301 ttttatctgt atcggaagtc tctgactttt tatctgctgt ttcgtctgac atgtttttac
    15361 atctgcctgc ttgttgctgt aagcgcatag tttattacaa taaatactcc aaatatgtca
    15421 ataattatcg cgaagcggat tgacgcggga gcgccgtcgt ggatacaact atatttatga
    15481 gcattcaaaa gcccttgcat aaaagacgac gcacaacagc cgttcgagca ggcgtgggga
    15541 cggacccggc ctataggtcg gtgatgccgc cgctttattt atcttccacc tttcagattg
    15601 acgggctcga caagcgcggg gattacgaat attcgcgcac gcgcaacccg acgcgcgatg
    15661 aacttgccaa cgctttggca gcccttgaag gcggtattgg ttgtgcggtt acagcttcgg
    15721 ggatggcggc gttgacgctc ttgttgcatt tgttagggcc ggatgatctg gtgctggcac
    15781 cttatgatat gtatgggcgc tcttattggc ttttgcgcgc actggcggag aaaaagcatt
    15841 tccggctgaa gtttatcgat tgttacgacg aaggcgcctt gacgcaagcc tttgacaaaa
    15901 acccgcgcat ggtgatttta gaaacgccca gcaacccggt catgcggatt gccgatattg
    15961 cagatatttc caaacgagcg aaggcctgcg gtgcactcac agtgtgtgat aacacattcc
    16021 tgtctcctat tttgcaaaat cctttaaagc tcggcgcgga tatcgttttt cattccacca
    16081 ccaagttcct gaacggccat agcgatgttg tggggggctg cgtgatcgtg aaagatgatg
    16141 agcaattttt ggccgattta catttatgga acaactcgct gggcaccatc ggggcgccgt
    16201 ttgatgcgta tatgacgctg cgcgggatgc gaacgctgga ggtgcgggtg caccggggag
    16261 aagagaacgc cgcagcgatt gcgcattatc tgcaagagca ttctaaagtt tccaaagtct
    16321 tttatcccgg tttgcccggc catgagggcc atgagatcgc aaaacgccaa caggaaggtt
    16381 ttggggcaat gctgagcttt acgcttgaag gcgatatgga cgggtttttg tcaaagcttg
    16441 agatgttttc actggcgcaa tcgctgggcg ggacggagag cctgatcaac catccagcca
    16501 gcatgacgca tgtttctatg ggcgctgaag cgcgtgcaaa agctggcgtt accgataatc
    16561 ttctgcgcct gtccgtgggg atagagcata ttgatgattt attaagtgat ttggaagcgg
    16621 cgctcaacgt tgcttaattt tatcgtcatt gcgagcgaag cgaagcaatc taagtggatt
    16681 gccgcgtcgg actaagtcct cctcgcaatg acgtttggta aatcattgcc ctctgctggc
    16741 agctaaaggt gattcgtaat ccgggcattg gccctgcaca aactggtatt cggccaaatt
    16801 atctatagct tgctgggctt ctgtgcgttc gatttccatt ttcttatatt caaactgaac
    16861 aagctgagca acggtgtttt tcattctgtc catgatggtt tgcgcatttt ccgctgcaat
    16921 gggcccggca ataaaaccgc gctgccggag cccgtcaaaa actttttttc cgatcgtttg
    16981 cgcatattta ttgacgattt tgcgatcagc attaacgtgt ttcatttcat gttcaagcac
    17041 ggcgccatac atgcaggaat cttctgaaac ttccttcgca attacaattt taggcgtaat
    17101 atcgagcgtt atggtgactt ttttataata gatgcaatag gcgtttgccc ttgatgtgag
    17161 cttgtaatca agctctacag atgagcgcat attgatttgc ccctccataa aaccgttcgt
    17221 atgcgtaacg ttgttgtagc cataagggtt aatcgtatcg atgctttggc cttgcagcga
    17281 tgcagcgcta cgtgaggtgt ctattactaa ttcagcggtt ttgggcacaa cagagatatc
    17341 agccgacgtc gtcatttggc aaatttgcgg gctggatacc gcaagaatta aggccgtgcc
    17401 tgctgttaca gggtcaaaag gaatcatgct gtctgcttgc tcttgttaaa ctcttccgaa
    17461 tagtatatca ggaaaaaggt taatatttga agaaattcag gaggttgcac catgggcatg
    17521 atcgcatatc tggtcagtct ggcctttgac atttatattg gcatcattgt tattcaggtc
    17581 gtggtgagtt ggctgattat ttttgacgtc atcaatgctg aaaacgcgca ggcgcaaaac
    17641 ctggttgcgc ttttgaaacg tgcgactgat ccaatttaca aacccttaca gaagtatatc
    17701 ccgccgattg gcggcattga tgtcactccg attgtggtta ttttggggct atcactgtta
    17761 caaaatatcg ttgtaggggt tttgatataa tgctggcaaa gaaagaggca cacgccatga
    17821 gcgagacaca gaatattgac ggcaaggcgg tggccaagac cctgcgcgag gaactggcag
    17881 atgaagtcgc ggggctgaat atacagcccg gtttggctgt gattttggtc ggcgaggacc
    17941 cagcctctca ggtttatgta cacaacaaaa ttaaggcttg tgaggcgtgc ggaattaaaa
    18001 gctttgaatc gcggttgcct gcacaggcca cagaagcgga gattatggcc gaaatcgaag
    18061 cgtttaatga taataaggat gtgcacggga ttttactgca attgccgctg ccttacccgc
    18121 tgaatgccga tgtgttgatc cagcatatca agccggaaaa agacgtggac gggctgacat
    18181 tcattaatca gggcaagttg attgcaggcg ataaaagcgg gttggtgccg tgtacgccgc
    18241 aaggctcgat gatgctcatc aagagtgttg aggaagattt gagcggcaaa catgcggtgg
    18301 tgatcgggag aagcttattg tttggaaagc caatggcgca gttgcttttg gccgaaaact
    18361 gtacggtaac aagcgcgcat tcacgaacgc aaaatttacc ggagatttgc cagcaggccg
    18421 atattctggt ggccgccgtc gggcgtccta aaatggttca gggtgcgtgg attaagccgg
    18481 gcgcgattgt gattgatgtg ggcattaatc gccaggatga cgggaaatta tgcggtgatg
    18541 ttgattatga acaggcacgc ggcgtagcga gcgcaattac gccagtgccg ggcggcgtcg
    18601 ggccgatgac gattgcgtgc ttgctgaaaa acaccgtcga agccgcgaaa aaggctaaaa
    18661 attgagggcg catagcatct ttcaatgatt tagccgaaat ctttttcttg ctatttatgt
    18721 gtttcgccct gtataaacat tgtttttatg taaatgttta taatgtgcca tggctagtaa
    18781 agcttttatt cgatccataa catcaatgag taaaaaagcg cttagcgagc gtccactttc
    18841 ccagcgtttt tccgatattg ctactcttgt cagaggtgta aggaacaatg ttccggaatt
    18901 ttcagaagat tccggttttg ccgttgaccc atgggacaag cataaaaatg tacgccctca
    18961 tgtagaagcg atgctgcata tatcggaaac aatccgccat tctcagacgt atctggattt
    19021 tttggaaaac agccgcgatc ttactttacg ctcgattcaa gatgaccgtg gtcttcttga
    19081 aagcatagtg cattatccgg tttcttgtga gaatgatcaa aaagcggtta ttcaagccgt
    19141 gaatttatat caggctgctg cgtcctgcga agcaagcgat tttgattttg gggagacgaa
    19201 tattgtattt tttgaagaag cgaagcgtta ctacaaacaa tccaatgcga ttcgtatctt
    19261 aaatggctcc tctgcttgta atctgaaaac cggatcgaga aaaattaaga ttaatacgca
    19321 tgacgatgcc ggctttcgta accctttaaa ggctttgagc atcgtttttc acgaaggcgt
    19381 ccatgaccag atttttcaac tggcttttgc ctgttacaca aagaaaggca atttggggaa
    19441 tttggctgga gatgccaagc tttatgcaac atttaaggaa cagggtgcga caattccttt
    19501 cggtatacgc ttggcttatc ttgaacaatt ttatgaaatc gtcgctcatg atcagcagga
    19561 taaattcgcg cgcgatctca ataatctttt gaaatcagcg gatagtggct ttactttgtc
    19621 acatccgcat taataaacca ttctcaggaa cggggtgtag ccctggattt gttcaagggc
    19681 atcgttgcgc gccttaaggt ggatggcata ttgctgtagc tgcatgaaat ttttgaatgt
    19741 catgtccgga tctttgaact cgtatgcgtc tacaccaatg ccatcaagat attcgaggag
    19801 tttacggtta ttgcccagcc agctactggc ctcgacaaag aaggcctgcg ctacaccgcg
    19861 atgatactta aaagagctac gaatggaagg ggaagccttc gtctcttcga tttcagcctc
    19921 aatggcactg gcttctttat caatttcttt atgccaatat ctaaggatga tttctgctgt
    19981 tttaatattt ttgagctgcc cttgaatgtt ttgagacgaa ttgagcacgg cctgtccgcc
    20041 gatatgggcc atatagcgcc gaatttcata gccatagagg tcatattcgg gaggaatatc
    20101 cacatagatg ccgtgaataa ggcgctcaat acgctcgcgc aacgtgcttg gcggttcctg
    20161 cttgatgaag cggaccttgt caaaaaccgg ttcaaggggt tgtttataag ggaatttgag
    20221 ctgctggggc attcccggaa cccgcggggc cctcatggct tcttcgagcg tttgcggcgt
    20281 tgccttctgg aatgatctgt aagccggacc attttgagcg aaaacctgcg atgcaggtgt
    20341 catagacccc gataccaata agcccagtgc tatcaatgcg tagagtagat gtctcatgca
    20401 tatattgtac aggtttttga cggtgatcgc caacagcgct tattcccctt gggcgaccgt
    20461 gaagccgggt ttgccgtcgt aattatgcag catgaccgat tttgtgaagt tgagatcggc
    20521 cttgttcatg cgatccatca gagcgatgac ctgcgaagtt tttacggttt cgccgttgtc
    20581 ggacataaag cgcaggcgcc agttatcggc gcagaatgtt tcaggctggc cgtcgggcca
    20641 gaccttggtg ccgcggttgg agatcatctg gagcttaaga ttgtcattga cgcaagcatt
    20701 gatagcggcg gccaagtcat cggcgctgcc atcccagttg atgccgatat cgacgccaac
    20761 gcgttctttc gaaacttcga cggatggttt aagcggcggc aatttgagtc cgccggcaac
    20821 atcggggtag gatttgacag gcagaacttc cggctcctgt cccagacgct caataacggc
    20881 cttggtgaaa ccttcggtgc caacctgttc ttttgaaata cctttgcggt aaatctcgcc
    20941 ggtatgaaca ccattttcga ttgtgcgcag ccaagcgttg tggattttgg ctgcaacgga
    21001 gccttggccg atgtgagtga gcatcatgac ggagccgaga ataagcccgg aggggttggc
    21061 gatgccctgc ccggcaatgt caggcgcaga gccgtggacg gcttcgaaca tgccgaaatc
    21121 aagaccgata ttggacgaac cgccaagacc gacggagccg gtgacttcgg cggctacatc
    21181 ggaaataata tcgccataca ggttcgtggt aacgatgaca tcgaataaat ccgggcggtt
    21241 ggcgacttta gctgtgccga tgtcgatgat catgtgttca ttttcaatat ccgggtattc
    21301 ggcggcaatt tcatcaaaga cctgatgaaa cagaccgtcc gagatcttca tgatattgtc
    21361 tttggacatg caggtaactt ttttgcgatt attggcgcgg gcatattcaa aagcgtagcg
    21421 cataatgcgc tctgtaccgg gacgggagat aactttgagc gaagccatta cgtcacgcga
    21481 ctggcggtat tcgatgccgc cgtaaagatc ttcttcgttt tcgcggataa tcaccagatc
    21541 cattttcgga tgattcgtgc gtacaaacgg gtggtaagat acgcaggggc gtacattggc
    21601 gaaaagccct aaggatttgc ggactgtcac attgagggat ttaaaaccgc cgccctgggg
    21661 ggtggtgatg ggggctttga ggaacacttt tgtccgacgc agggattccc atgattcttc
    21721 tgcgataccg ttttttattc cagatttata gaccgcttca ccaatttcta tttcttcaat
    21781 ctcaagctgc gcaccggcag cctcaagaat ttgcaaagtc gcgtccatga tttccggacc
    21841 gattccatcg ccgcgcgcta ctgtgattgg tgttttggtc atgattgttt tccccttaaa
    21901 gaacctgaaa attcgcctga gaatcgtagc atcatagcgg cataaatcta agcgttttta
    21961 tttcttttca tacagacaca ttcgagattt ttcggaaaaa tctgttatta cgtttaattt
    22021 tctgttgcat tgcaatgtta agaggagcat gttccgcgat aatttttaaa ttcatttgat
    22081 attaacgtca aaaaaagaaa ggcaaaatat catgccctgc aatgatgcaa tgattgaaga
    22141 tgtcgtgaca gcccgtccaa accaaacagt ttccgaggtg ctcgaacttt ttagcaaata
    22201 caatatccga agcgttccca tcgttgatga aaatcggagc gttgtcggcc tgttcagctt
    22261 ttcgcacctg ttgtacagcc tacttcccgt tccggcgacg ctcggcgatc acatgatgcg
    22321 gctcaaacac atggatatta gtctcgatca cctgctgggc gcttctccct gggttgcaaa
    22381 acggttgaac attattttgc cgaaaacgat ggaagaagtc atgttaaaaa atcccgtctg
    22441 cgtccgggtc gactcaccga tacgcgaagg cattcgcctg atggttaaat atgacagccc
    22501 cctgccggtt gtggaggacg aaaaaaaccg gaaactggtc ggcctgattt cctcccaatc
    22561 cgtgcttggc gttctaacgg aaatcgcttc acatctggaa aaaggcaaag aagtgcacga
    22621 ataaactgca ctcaaagttt ggaaatcgtt taattcaaaa caaaaaaaga aagtcacaag
    22681 ataatgcatg aattagtatt tgatacgccg atgcttattt ctgcgatcat tctgatcgtg
    22741 acctttgcag gcatttttac cgaaggactt caccatatcc accgcacaaa aatcgcgatg
    22801 ctcggcgcag cggccatggt gatcatcggg caggtcttcg ggttttataa cccggaaaaa
    22861 gccattgaag ccatcgactg gaacgtggtg ttcctgctcg gcgcgatgat gaccatcgtc
    22921 gccatcatga tcccgacggg tgggtttcag gccatagcct actggatcgc ggatttcagc
    22981 aaggggcggc tgtttttgct tctggccctg atgggaacgg ccgtaacggt catttccctg
    23041 ctgctggaca acgtgaccac ggtggtgatt ttcggcccgc tgatcgtcct gatctgccag
    23101 gcgctgaagg tcaacccgat tccctatctt ctggccgccg cgcttctgtc cgatacgggc
    23161 ggcgtggcca cgctggtcgg ggatccgccc aacctaatga tcgg
//
LOCUS       DAOSWR010000014        22065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1500_length_22065_cov_5.485279, whole genome shotgun sequence.
ACCESSION   DAOSWR010000014 DAOSWR010000000
VERSION     DAOSWR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 22065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22065
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_22065_cov_5.485279"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK513_02065"
     CDS             <1..612
                     /locus_tag="PK513_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81270.1"
                     /translation="YDYLRERLQKMGLSEEAIPKAGQNIHITPQLKLLLNDDGLEIIK
                     DLIHEKFPYAPPDIIVIDPIRNVFDGGPDFGNLGENDNNAMLFFLQQRVEMLRDMINP
                     RAGIILVHHTKKISKAMFKDDPFQALSGASSLRSYYSSGMLIFRPDEAMDTKSLHFEL
                     RNGPGIANMHVAKRGGVWTEIDPNTQPLVRQDYSNKLKSERHR"
     gene            625..1011
                     /locus_tag="PK513_02070"
     CDS             625..1011
                     /locus_tag="PK513_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81271.1"
                     /translation="MQRLLEDAQFDQKLYTIESFSEAYEDIDGLGSQTTIKKRISVLM
                     GKGYIKCLKTPHPLTKQTAERSKYGYLVVENMQLGQDEIDEDTGEIVTLAIAVLPTHY
                     KDEGSGGVRQVENAQSWVYHDEEKAR"
     gene            complement(1572..3326)
                     /locus_tag="PK513_02075"
     CDS             complement(1572..3326)
                     /locus_tag="PK513_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO81272.1"
                     /translation="MLALLCGQTYILSANAQITESAQSVNAVEESPQIPQDLLGRSTP
                     RGTIQGYVEAIARENYEKAAQYFDLSYVPKRKRDEKAAEIARNFQRLLNFNGTLLPNN
                     FFSDKPSGRADDFLEDNLDKAGSFILEGETVPVLLERVEQAEHGPIWLFSAQTLGKVP
                     DTLDIADISRLEKYFPRVLLENKWDGVSYAHWLMIAILIPLSFLVAWLTAKIGIFIMR
                     RIVHKFWPNRHTGLVEAFALPFCLFSAAWLFSTSTQKMGISIIARQNFSEITVIVAWI
                     AFVLLLWRLVNVFAEVGIHRTRYRGDKGRLSAVLFFRRVAKFVLIGVGCISVLDIIGV
                     DVTTGLAALGIGGLALALGAQKTIENLVGSLTVIFDQPVRVGDYCQVGEIAGTIEQIG
                     MRSTRIRTLDRTVVTIPNGDFSAQKIENYALRDMFRYRHILGLRYETSPDQIRYLLVE
                     LRKIFYAHPRVNDDPARVRFLGFGASSLDIEIFAYVRANDYNEFLEVQEDLNLRVFDV
                     VNESGSGFAFPSQTIYMAQDQGLDKAKTKTAEKQVKDWIANNDLQIPRFDFETIKDLE
                     DTLSYPPEGSGMKKTDPK"
     gene            complement(3362..4681)
                     /locus_tag="PK513_02080"
     CDS             complement(3362..4681)
                     /locus_tag="PK513_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81273.1"
                     /translation="MIKNKFILLAVLPLLLFPLSGCSSIAKGVTEAVLERKGEDSRQC
                     EIAGAEFDGVTQSIEAQEIHPKKVTKVLMVHGISTHLPGYSNRFQKKLYDTLNLNKTD
                     EIVKTIRLDSDEIEWGDEDHVLGELHITRHMNDEQTRELIFYELTWSPITTPQKESLA
                     ADSANTEGLPRSNLNGSLKSFMNATVPDMLIYNGNGYEKITKAIEESVCWMLADDWDT
                     LPESGAHSCAAWKGSTFASILQDDHFFVTHSLGSRITIDTIQNFASRKTTPNKNMRQS
                     EIARKVRQKEFTVFMMANQLPLLQMGRNAPDVPEDQAAYCSAGGKYGEQRVMKKMNIV
                     AFSDPNDILSYPIPTNFAQEYIDSRICPSVVNVSLNVAPEQDVFGTFSFANPALAHNG
                     YIEDDRVITLIANGMHRNRPSPLIEKSCKWMKIEGLPAKTAKPSGDS"
     gene            complement(4668..5627)
                     /locus_tag="PK513_02085"
     CDS             complement(4668..5627)
                     /locus_tag="PK513_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOO81274.1"
                     /translation="MLIGAAWWYTLPVSEIIKIYILIFGGLFGLGVIVADFTRNRWRS
                     RIALSVAFVLTTLWWMIIAPSNDRIWKDELSRTVHGDVNGNIVTLYDIRDFTWHGSDK
                     EYDQKWATQAYDLESLESIDLFLSSWSSPLIAHTLVSFGFDDGRHVVFSVSIRKERDE
                     EYSPVAGFFKQYELALLAGQERDMIYLRTHIRKEDVSLYRVHMNAEQRRALFLSYVQR
                     GNALRDNPEFYNTITENCTTVPYQMIKTFVPGLPADYRVLFSGLLPSYIYDLGGFGHD
                     LSLEELQSRAHISASAQGYAPESGIDFSEFIRQPVKDFPDDQK"
     gene            complement(5735..7024)
                     /locus_tag="PK513_02090"
     CDS             complement(5735..7024)
                     /locus_tag="PK513_02090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO81275.1"
                     /translation="MPLEPSKQDKRPCVAIIGAGFAGLAATKNLADADADIILIDRRN
                     HHIFQPLLYQVATAALSPAQIAQPTRLILRGQKNCTTALAEVSGIDPDKKVIYGPKGD
                     LAYDYLIVATGATHTYFGHNEWASFAPGLKSIEDALSLRGRLLDAFEEAETSSDSDSQ
                     KALLTFVIVGGGPTGVEMAGAIAELARHTLPGEFRRIRTDSARILLIEAGPKVLAPFP
                     SHLSQRALQDLQDLGVEVRTGQAVTNCTAEGVYIGEEFIPARTIIWSAGVKASPAAEW
                     LKAECDRAGRVLVNPDLSLPGHDDVFVIGDTAHLTDRNGVAVPGLAPAAERQGHYVAR
                     LIESRLQNKPAPEPFAYKDYGIMATIGRGKAVGKVGSWTFTGRLAWSLWGLVHLMPLV
                     GFRNRMMVAGDWFWAYFTKARSVRLITVKKNDSVELK"
     gene            complement(7050..7307)
                     /locus_tag="PK513_02095"
     CDS             complement(7050..7307)
                     /locus_tag="PK513_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81276.1"
                     /translation="MSAQQKDDIFALGENLQETQKKLQEFGKEQSAFLTGLVEKNMNF
                     QKEQYDLWSKIIQNQVAHNNNILQDYVKIAGGSVKKASPSK"
     gene            complement(7356..7607)
                     /locus_tag="PK513_02100"
     CDS             complement(7356..7607)
                     /locus_tag="PK513_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81277.1"
                     /translation="MTDTDHKKYKNLLNEILELRDETRLKILLGSKDLQDEWHELEEK
                     WEKFYKDADLAKSGQDITSAFGIVGEELKNAYKRIKGAL"
     gene            complement(7604..8074)
                     /locus_tag="PK513_02105"
     CDS             complement(7604..8074)
                     /locus_tag="PK513_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HOO81278.1"
                     /translation="MSALYKTQVIATEGRHGHVKSSDGLLDLALAIPKEMGGKGDATN
                     PEQLFAAGYAACFENAVIHLAKKQKIQIKDADVSVIADISLHANDQEGFDLAVSLTTI
                     IHGLSQRDAEELVQAAHAVCPYSNAIKGNIDVVLNTQGKPFSQTLGLNYKGKKA"
     gene            complement(8086..8808)
                     /locus_tag="PK513_02110"
     CDS             complement(8086..8808)
                     /locus_tag="PK513_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HOO81279.1"
                     /translation="MARVVLITGGTTGIGAKTALMFKENGYQVVCNYIGNKEKAQSFS
                     ATTGIAVYEWDVTDPDACEAGFKKISDDFGPVSILVNNAGIIKDHTITKMTVEEWKSV
                     LNVDLNACFYMARQAVPAMKEQKFGRIISLSSVNGLAGQFGQTNYAAAKAGIIGFTKS
                     LALETARYGITANAVAPGYTDTSMMQAVPDETLQKIIDKVPVGRLAKPEEIARAILFL
                     AHDESGFITGETLSVNGGLYMQ"
     gene            complement(8884..9645)
                     /gene="fabI"
                     /locus_tag="PK513_02115"
     CDS             complement(8884..9645)
                     /gene="fabI"
                     /locus_tag="PK513_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOO81280.1"
                     /translation="MSKKPLKGLIVGFANEQSIAWGCIQAFDALGAEMAVTYLNEKTR
                     QYTEPLMAKINAPLYLPLDVTDEAQMDAVFSSVKKQWGGLDFLLHSIAFAPKEDLQGR
                     VLDSSSAGFQMAMDISCHSLIRLAKRAEPLMKDGGSILTMSYYGAEKVIENYNLMGPV
                     KSALESSMRYLAHELGPRNIRVNALSPGPVMTRAASGLSHFDALMEKAAKEAPIHALV
                     SIEQIGEAAAFLVSDKSRHITGQTIYIDNGYSVHA"
     gene            complement(9647..10405)
                     /locus_tag="PK513_02120"
     CDS             complement(9647..10405)
                     /locus_tag="PK513_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO81281.1"
                     /translation="MTQKTQQRFLGKTAVITGAGSGIGEATATLFAQEGAKVIACDIS
                     QARLDALSRTLEAYDIKTCAGDVCNGKVIEEIVKMAQERIDVLANIAGIMDRFLSVGE
                     IDDSTWDKVIAVNLTAVMKLTRATLPIMLEAGHGSIVNVSSEAGLRGSVAGAAYTASK
                     WAINGLTKNTAFFYGDKGIRVNAVAPGAVKTSIEAPMESNLSINRIEPLMKALMPPTA
                     EAIEIAQAIAWLSSDEAKNINGVILPSDGGWGAV"
     gene            complement(10402..11514)
                     /locus_tag="PK513_02125"
     CDS             complement(10402..11514)
                     /locus_tag="PK513_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOO81282.1"
                     /translation="MPHLLTLNTGSSSVKFRLFGLEKTLPLLAGGKISGIGGTPVFDV
                     SKENGVPETYPLSEGLSHEEAIDLVLKWLEDNRLPTSVVAHRIVHGGEQYAAPVKLDE
                     GAMKYLRSLEPLAPLHQPHNLAAVDILQSRQPGLPQMGCFDTAFHARHGRLFNEFALP
                     QSIRDQGVKRYGFHGLSYDWIAHCLKTDYPHLVEGKVVVAHLGNGASLCAMQNGRSID
                     TTMGMTALDGLPMGTRCGALDAGAVLYIIRTLGLSPDETENILYNESGLKGLSGLTND
                     VKVLSESTDKKAAFALEYFALKTAQYIAAMATSLGGMDALIFTGGIGENSEKIRIMIL
                     RHLSFLPPFEHHIIPANEERAMAIEIWRNFQKEMHP"
     gene            complement(11514..12923)
                     /locus_tag="PK513_02130"
     CDS             complement(11514..12923)
                     /locus_tag="PK513_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOO81283.1"
                     /translation="MAEDIMANVTFEELEIGQSAELKRRLTQKDIELFAAVSGDVNPA
                     HMDEHYAENSLFHGVIGHGMWSGGLISTVLGTELPGPGTIYMEQDMRFKKPVMLGDEV
                     TVRVSVKSKRDDKPVVVFDCICLNEKGEVLVEGTATVLAPTEKIRIPRPDIPWVDIRE
                     KDHYKGTMEKCRAFGILKVAVVHPVQANVIEAAAEAVEENLIVPVLIGPKERIEAAIK
                     ESGVNAKGWQIINTEHSHAAAAKAVEMAVAGEVGAIMKGSLHTEELLEAVVPSSAGLR
                     TERRISHVYLMNIPTYHKPLMITDAAINIAPNLEQKADICQNAIDAWHVLFNHEHGDP
                     KVAILSATEEVTSRIPSTIDAAALCKMADRGQIKEGRLDGPLAFDNAISAQAVKDKNI
                     ISDVAGDADILVVPDIEAGNALAKQLIFLGKADAAGIVLGARVPIILTSRADSKRTRM
                     FSCGVAMALSAARQEGKIK"
     gene            complement(12935..14656)
                     /locus_tag="PK513_02135"
     CDS             complement(12935..14656)
                     /locus_tag="PK513_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO81284.1"
                     /translation="MTNTPIEQFTSAMDDLDDFFQMKLAHLTYGLSPAGLVQMYATWL
                     SQLSLCPGRLASLAAFPSVKLAARLLQGCDDDNPACADDPRFSSESWKIWPWAMYLQT
                     FHAMEEFWDLATKDIPGLDPQTERAVSFMARQILDARAPSNFLLTNPDLLFETVRSSG
                     ASLIQGAVNATEDFKRNINSQPPAGIENFKPGEHLAITKGTVVFKNDLMELIQYDPLT
                     PKVSKEPVLILPAWIMKYYILDLSPHNSLVKWLTEQGHQVFIVSWKNPTSEDRSLGMD
                     DYVRDGALTAVDAVSSITNNAPIHLTGYCLGGTLAMITAAYMAHEKDERLKSLSLFAA
                     QGDFTEAGEMMVFVTPGEVDYLEKMMKGQGYLDTKQMAGAFQMLRSYDLIWSRMVKEY
                     LMGQRGELFDIMAWNADATRMPYKMHSEYLERLFLNNEFANGRYSVLGKAIAPENITL
                     PVFAVGTEKDHVAPWKSVYKIHLMVGNEVTFVLTNGGHNAGIVSEPGHKYRHYHMAST
                     KPGSAYEAPDEWLENAKDFEGSWWVAWDKWLKNRMGKEEIAAPKKPGNDKFKPLYDAP
                     GKYVHQA"
     gene            14748..15383
                     /locus_tag="PK513_02140"
     CDS             14748..15383
                     /locus_tag="PK513_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO81285.1"
                     /translation="MDTMQIRQQREDMSKESAILEAATHSFLRKGFSGVSMEEIARES
                     NVAKQTLYSYFKGKDDLFRAIIHAQSDAFFATLPHNDDQTQNIEEFLHIMGERAMDML
                     FKRDTLALYRVIVSETPRFPELGKFYWQSGAQAFTDRLSHFLIARGFPKGKAQSATEQ
                     FLSFMFGTLLTKTLLNKEYHPSKEEKKAHIEQAVSAFFMLNGQYVTEKVNP"
     gene            15380..16375
                     /locus_tag="PK513_02145"
     CDS             15380..16375
                     /locus_tag="PK513_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO81286.1"
                     /translation="MKKKNTFIRLTIILALAAILGGGYFFWQAQQSSLPEFIASGNGR
                     IEAEETHVAAKYAGRVIDVLFDEGDMVKQGDIIAHIDAEDLEAQHDGAIANLSSAKNA
                     VAEAQAGVIHREAEMELAQAEEQRAKALVGTGVSKELYDQRKASVKTAEAALNVAKAQ
                     LASAENQVETAQSEIDRLNVLIKDATLKAPMSGRVQYRLAEPGEVLSAGSRVVTILDL
                     ENVYMTIFLPAKDIGKIIVGSEARIVLDALPDVVIPAKVSFISSEAQFTPREVETREE
                     REKLMFRVKIRIPKSLLSKHIEKVRTGLPGIGYVMLGDTNDWPEHLKMNIGQSQE"
     gene            16385..19135
                     /gene="rbbA"
                     /locus_tag="PK513_02150"
     CDS             16385..19135
                     /gene="rbbA"
                     /locus_tag="PK513_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HOO81287.1"
                     /translation="MQERSVITLSHVTHVYGKAQALDDVSLEIPAGKMIGFIGPDGVG
                     KSTLFGLVAGARKIQSGEIKVLGQDIGNDKKRKDLLPRIAYMPQGLGKNLYMDLTVFE
                     NLDFFARLFGQGKAEREARIKTLLKATGLDPFPDRPAGKLSGGMKQKLGLCCSLIHDP
                     DLLILDEPTTGVDPLSRRQFWDLIRSIRKDRPQMSVLIATAYMEEAEGFDWLAAMNDG
                     KILDTGTAEELKDRTGGGTLEEAFIALVDDGKQVKHEKLVLPPYEDGHGEDPVIVTKN
                     LTKKFGDFTAVNNVSVEIMPGEIFGFLGSNGCGKTTTMKMLTGLLPASEGEAKIFGKT
                     PSNDDMEFRHRVGYMSQAFSLYSELTVWQNLELHARLFHVPDDTIKPRIDELLRRFGL
                     ETHKDTLPSSLPLGVRQRLSLAVAVVHEPEMLILDEPTSGVDPVARDEFWKLLIDLSR
                     NKKTTIFISTHFMNEGMRCDRITLMHAGNILACDAPDKLIEGKGKDTLEEAFIAYMEE
                     ANAEKGGKPDSSAASDITASVADDKKTGASLALVQGGRRIAAYAMREAKELSRDRIRE
                     AFAFFGTMILMLVFGFGITFDVENITFAVLDQDKTPESRLYTQALEGSRYFTEKAPLI
                     DHADMDHRLKSGQITLALDIPPGFGKNLLRQKSPEVAVWVDGAMPFRAETAGGYMQGL
                     HYDFIGRLYEDYYGEQVALSPVSIEPRFLYNQGFKSAYAIVPSVIALMLLFIPSILAA
                     LSVVREKELGSITNLYVTPTSKFEFLLGKQIPYVAITMMNFLLLVLTAWLVFGVPVKG
                     SFIALFIGAFLYAGCTTALGLFFSAFTGTQIAALAVTAIMTLLPAVQFSGLMQPVSTL
                     EGVGAFIGRIYPTTHFITVAKGAFAKDLDFAELWPSLRALLISWPIILTAGIFFLKKQ
                     EA"
     gene            19139..20260
                     /locus_tag="PK513_02155"
     CDS             19139..20260
                     /locus_tag="PK513_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81288.1"
                     /translation="MRLSVKNIYRLGIKELRSLYRDPVMLFMILWAFSASIYIAGTSI
                     SHDLHNASIAIVDEDQSPLSLRIRSAFLPPYFKQPDIIAFQDIDEGMDLDKYSFVLVI
                     PEEFEADVLRSRQPTLQVNLDATAVMIAGIGSGYIQNIINDEITRFTNNGPSTSQSPV
                     DMRLRYAFNPNLSSKWFTSIMEVISNITMFVILLTGAALIREREHGTIEHLLVMPVTP
                     IEIMLSKVWANGLVILTATAFCLWVMIEGVLQVPIAGSVPLFLLGTLLYLFFASALGI
                     FLGTTARSMPQFGLLFIITILPMNLLSGGQTPLESQPDWLQHIMWFVPSTHFVSFAQA
                     ILYRGAGLDIVWPNFLAVSVVGLSLLTLSALAFRRSIAA"
     gene            20346..20552
                     /locus_tag="PK513_02160"
     CDS             20346..20552
                     /locus_tag="PK513_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81289.1"
                     /translation="MKRKAILMTGLIAIMLMSGCAGGMRQDGVKQKVQDEFVFENIDK
                     NADGNIDPQEFAAFKQNLKSLKRY"
     gene            complement(20580..21572)
                     /locus_tag="PK513_02165"
     CDS             complement(20580..21572)
                     /locus_tag="PK513_02165"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HOO81290.1"
                     /translation="MSHAYANEQDNNFKDRIPKDLLNEDADFFTFTIENDNFGGNTDE
                     NYTSGVRLTYFDYGADPPWFADILDHYVPTFEINETTSTYYSFGQNLYTPENITVRTP
                     DPKDRPYAAFLYVSAGLTSLTDNHVDDLEATIGIVGPWALGEQTQKFVHDVLDADDPS
                     GWDHQLENEPGLMLSWQRQWPEAYAADIDGFTFRAAPHAGITLGNIYTYGSGGLSFQL
                     TPSQYKWQSTPLRVRPAIPGSGFFAVPEDRFAWSLFAGVEGRAMGRNIFLDGNTFEDS
                     PSVDKKYLVADANAGVSFTYGKAQFSYTINWRSKEFDGQDDPSLFGAISLGYRF"
     gene            complement(21635..>22065)
                     /locus_tag="PK513_02170"
     CDS             complement(21635..>22065)
                     /locus_tag="PK513_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOO81291.1"
                     /translation="LHRPFVDMILEDLEKDHPEPMVQLRDLCIQIMLDIEKDKQRQQT
                     LILFFMKSDYSGDLAPYKDKHREKKAESMRLFKRYIEKAKTAGKLNTQADPELLTQCI
                     SCFMKGILFEYLDNPEGFDIENKAPKLITLFFSNLGLEKG"
BASE COUNT         5489 a         5496 c         5357 g         5723 t
ORIGIN      
        1 tatgattatc tgcgcgagcg tctgcagaaa atgggactgt ctgaggaagc cattcccaaa
       61 gccgggcaaa atatccatat cacgccgcaa ttaaagctgc tgctgaatga tgacgggctg
      121 gagattatca aagacctcat ccatgagaag tttccttacg cgccgccgga tattatcgtc
      181 atcgatccga tccgtaacgt gtttgacggc gggccggact ttggcaatct gggggagaat
      241 gacaataacg ccatgctgtt tttcctgcag caacgggtcg aaatgctgcg cgatatgatc
      301 aatccacgcg ctggcattat ccttgttcac cataccaaaa aaatcagcaa ggccatgttt
      361 aaggatgacc cgtttcaggc cttgagcggt gccagttcgc tgcgcagtta ttattccagc
      421 ggtatgctga tcttccgccc ggatgaggct atggatacga aatcgctgca cttcgagctg
      481 cgcaatggcc ccggcattgc caatatgcat gtggccaaac gcggcggcgt gtggacggaa
      541 attgatccga atacccagcc gctggtgcgc caggattatt ccaacaagct gaaatccgaa
      601 cgtcaccgct agcacgatca gatattgcaa cgcctgctgg aggatgcgca gtttgatcaa
      661 aagctctaca caattgagag cttcagtgag gcgtatgagg atattgacgg gctgggcagc
      721 cagacgacta tcaaaaagcg gatcagcgtt ctcatgggca agggctatat caaatgcctg
      781 aagaccccgc acccgctgac gaaacaaacg gcggagcgca gcaagtatgg ctatctggtc
      841 gttgagaaca tgcagctggg gcaagatgaa atcgatgaag atactggtga gattgtgacc
      901 ttagccattg ctgtcctacc cacgcattat aaggatgagg gctccggcgg tgtgcgtcag
      961 gtcgagaatg cgcagagctg ggtttatcat gatgaggaaa aagccagatg aatctgcggc
     1021 agatctgccg ggcggtgata tctgcttttg ttttattaca agaacttacg cgcagagcag
     1081 aaattctggt acatctgcat ctgccgtctg catttctggc tataaggctt ggtgtgtata
     1141 ggcttacggc atataagcag atctcactct ctccacccct ccctaaaggg agggggcgtg
     1201 ggatacagat ccacgcaccc catcccggtc ggttaaggtt cgatgccgag cagcatatga
     1261 ataacatccc aaaaaagaat ccgaacttct gaatccgtat gtttgctggg atcccattct
     1321 tcactgaagg taaaaaagac agcgaaatct gatacaccca aaaggatgca gggttctcaa
     1381 tcatgtaacg cactgtatcg tgccagggca tttcctatag gggcggggaa cgcacgggat
     1441 ttttttagag acagggcttt caacttagtg gacaaaatgg tttcggttag cagttaacta
     1501 tttgatttat atagaaaact gccgaaacga aatcacagct tggctgacag gcggcatatc
     1561 catgagatat ttcatttagg atcggttttt ttcataccag atccttctgg agggtaggat
     1621 aaagtatcct ccagatcctt gatcgtttca aaatcaaacc gcggaatttg cagatcatta
     1681 ttggcgatcc agtctttgac ctgtttttcc gccgttttgg ttttggcttt atccaatccc
     1741 tgatcttgcg ccatatagat ggtttgtgag gggaaagcaa agccgctgcc gctttcgttc
     1801 acgacatcga aaacgcgcag gtttaaatct tcctggactt caagaaattc attgtaatca
     1861 ttggcacgga cataggcgaa aatttcgata tcgagcgagg atgcaccgaa gcctagaaac
     1921 ctgacccgcg ccggatcatc attcactcgg gggtgcgcgt agaaaatttt acgaagctcc
     1981 accagcaaat aacggatttg atcaggggag gtttcatacc gcagccctaa aatatgacga
     2041 tagcggaaca tatcacgcag agcgtagttt tcgattttct gggcggagaa atccccgttg
     2101 ggaattgtga ccacggttct atccaaagtg cggattcgtg tcgagcgcat gccgatttgc
     2161 tcaatcgtcc ccgcgatttc tccgacttgg caataatcac cgacacgcac gggctgatcg
     2221 aagattacgg tcaagctgcc gactagattc tcaatcgtct tttgtgcccc taacgctaaa
     2281 gccagacccc cgatcccaag agcggccaat ccggttgtga catccacacc gataatatca
     2341 agcacggaaa tacagccgac accaatcaaa acaaatttgg cgacgcgccg gaaaaacagt
     2401 acggcggata ggcgcccttt atcgccgcga taccgtgtcc ggtgaatacc aacttcagca
     2461 aatacattca ccagccgcca aagtaaaaga acgaaagcaa tccaggcgac aattacggtg
     2521 atttctgaaa aattctgccg cgcgataatc gaaatgccca tcttttgcgt gctggtagaa
     2581 aacaaccatg cggcggaaaa caggcagaat ggaagcgcga acgcctcgac caatccggta
     2641 tggcggttcg gccaaaattt atgcacgatc cggcgcataa taaaaatgcc gatttttgcg
     2701 gtcaaccaag cgaccagaaa agacaaagga attaggatag cgatcatcag ccaatgagca
     2761 taggagacac cgtcccattt gttttctaac aaaacacgtg ggaaatattt ttcaagccta
     2821 ctgatatctg caatatctag cgtatcgggg acttttccca gagtttgcgc tgaaaaaagc
     2881 cagattgggc catgttctgc ttgctctacc cgttcaagca gaacaggcac ggtttcgccc
     2941 tccagtataa aactgcctgc cttatcaagg ttatcttcca gaaagtcatc tgctcgaccg
     3001 gatggcttgt cgctgaagaa attgttaggc aataacgttc cgttaaaatt taagagcctt
     3061 tggaagtttc tagcaatctc ggcagctttt tcatcccgtt tgcgctttgg gacatagctt
     3121 agatcaaaat actgtgcggc tttttcgtaa ttttcccggg cgatagcctc cacataacct
     3181 tggatcgtac cgcgcggtgt cgatcgtccc agcagatctt gagggatttg cggcgactcc
     3241 tcgactgcgt ttacgctctg cgctgactct gtaatctgcg catttgcgga taatatgtat
     3301 gtctgcccgc ataataatgc gagcaataaa agaaggagtg tccgttttat gtgcgccata
     3361 tttaactgtc tcccgatggt ttagccgttt tcgcaggtaa tccttcaatc ttcatccatt
     3421 tacagctttt ttcaattaaa ggcgacggac gattacggtg cataccgttt gcaatcagtg
     3481 taataacacg gtcgtcctca atataaccgt tgtgagccag tgcggggttt gcaaaagaaa
     3541 atgtcccgaa tacatcttgt tccggggcaa catttaggct cacattcaca acggaagggc
     3601 aaatccggct gtcgatgtac tcttgcgcga aattcgttgg tataggataa gacaaaatat
     3661 cgttggggtc gctaaaggcc acgatgttca tttttttcat gacacgctgc tccccgtatt
     3721 tgcctcccgc gctgcaatat gcggcctgat cctccggaac atcaggcgca ttacgtccca
     3781 tttgcaataa aggaagctgg ttcgccatca taaaaactgt aaattccttc tgtctgacct
     3841 tgcgtgcgat ttcgctttgg cgcatatttt tattgggggt cgtcttgcgg gaagcgaaat
     3901 tctggatcgt atcaatcgta attcggctgc ctagggaatg cgtcacaaag aaatgatcat
     3961 cttgtaaaat gctggcgaat gtcgaacctt tccatgcggc acagctatgc gcaccgcttt
     4021 ccggcaaagt gtcccaatca tcggcaagca tccagcagac ggattcttca atggccttgg
     4081 taattttctc atagccgtta ccgttataaa tcagcatatc gggtacggta gcgttcataa
     4141 aacttttaag ggatccgttc agattgctgc gcggcaagcc ttcagtattg gcggaatcag
     4201 cggccaaaga ttctttttgt ggggttgtga tcggcgacca ggtcagctcg taaaatatca
     4261 gctctcgcgt ttgctcgtcg ttcatatggc gtgtgatatg aagctccccc agaacgtgat
     4321 cctcatcgcc ccattcaatc tcatcgctat ccagcctgat cgtttttacg atttcatcag
     4381 ttttgttaag gtttagcgta tcatagagct ttttctggaa acggttggaa tatcctggca
     4441 aatgggtgct aatcccatgc accattaaaa ctttcgtgac tttcttcggg tgtatttcct
     4501 gcgcttcaat gctttgtgtc acgccgtcaa attccgctcc tgcgatttcg cattgcctgc
     4561 tatcttctcc tttgcgctcc aaaaccgctt cggttacacc tttggcgata gagctgcagc
     4621 ccgagagcgg gaataacagc agcggcaaaa cagctaaaag aataaattta tttttgatca
     4681 tcagggaaat cctttacagg ttggcgaata aattcagaaa aatcaatgcc gctttcaggg
     4741 gcgtaacctt gcgcagaggc cgagatatgg gcgcgggatt gcagttcttc taagcttaaa
     4801 tcatggccaa agccgcccag atcgtaaata taagacggca aaagtccgga gaacagaacg
     4861 cggtaatcag ccggaagacc aggcacaaac gtcttgatca tctgataggg aacggtcgta
     4921 caattttcag taatcgtgtt gtaaaactcc ggattatcgc gcaaggcgtt gccgcgctgg
     4981 acataggata aaaacagcgc acggcgctgt tccgcattca tatgaacgcg gtaaaggctg
     5041 acatcctctt tacggatatg tgtacgcaaa tatatcatat cccgctcttg cccggccagc
     5101 agcgcaagct cgtattgttt gaaaaatccg gcaacgggtg aatattcttc atcacgctcc
     5161 ttgcggatgc tcacggaaaa aaccacatgg cgaccatcat caaagccaaa gctgaccaat
     5221 gtatgagcga taagagggct tgaccagctg cttaagaaca aatcgatgga ttcaaggctc
     5281 tccagatcat acgcttgcgt agcccatttt tgatcgtact ctttgtccga tccatgccat
     5341 gtaaagtcgc gaatatcata cagtgtaacg atgttgccgt ttacatcgcc atgcaccgtt
     5401 ctactcaact cgtctttcca gatacgatca ttggacggcg caattatcat ccaccaaagg
     5461 gtcgtcaaaa caaaggctac agacaatgct attcttgagc gccagcggtt gcgggtaaaa
     5521 tcagcgacga taacgccaag gccgaacaac ccaccaaaaa tcaggatata tatttttatg
     5581 atttccgaaa cgggaagcgt gtaccaccac gcggctccta tcagcaagca tatggctgct
     5641 attaataaga tgcgcagtac tgctatcagc aaaaccgtga ctttttttaa gttatgtagg
     5701 cacggctttt ggataatatg cgtttgttca tagatcactt cagctccacg ctatcatttt
     5761 ttttaaccgt aatcaggcgg acactgcgcg ctttggtaaa atacgcccag aaccagtctc
     5821 cggcgaccat catcctgttt ctaaaaccga ccagaggcat caggtgaacc agcccccata
     5881 acgaccaggc aagacgtcct gtaaacgtcc atgacccaac tttgccaacg gccttgccac
     5941 gtccgatcgt tgccatgatc ccgtaatcct tgtacgcaaa cggttcggga gcgggcttgt
     6001 tttgcagtct gctttcgatc aaacgggcta catagtgccc ttgtcgttcg gcagcaggag
     6061 ccagtccggg gacggccaca ccgtttctat cggtaagatg agccgtatca ccaatgacaa
     6121 agacatcatc atgtcccggg agggataaat cgggattgac cagaacgcgt ccggctctgt
     6181 cacactctgc cttcagccac tcagccgcag gggaagcctt aacgcccgcc gaccagataa
     6241 tggtgcgcgc cgggataaac tcttcaccaa tataaacgcc ttccgccgtg caattcgtca
     6301 cggcctggcc ggttctgacc tcaacgccca gatcctgtaa atcctgcaaa gcgcgctgcg
     6361 ataaatgact tgggaaaggc gccagcactt ttggcccggc ctcaatgagc aaaattctgg
     6421 cgctgtccgt tcgaattcgc cggaattcgc cgggcaaggt atggcgcgca agttcggcaa
     6481 tcgcacctgc catctcaaca cctgtcggtc cgccgccaac gattacaaaa gttagaagag
     6541 ctttctgact gtcagaatca ctactggttt ctgcttcctc aaaagcatcc agcaatcgtc
     6601 cgcgcaagga taaagcatct tctatggatt ttagtcctgg cgcaaaggac gcccattcgt
     6661 tatgaccgaa ataggtatgg gtcgcgcctg tggcgacaat gagataatca taagccagat
     6721 cgcctttcgg cccgtaaatc acttttttat cgggatcaat gccgctgact tccgctaacg
     6781 ccgtagtgca gttcttctgc ccgcgcaaaa taagacgcgt gggctgcgcg atttgagcag
     6841 gagataaagc agccgttgcc acctgataga gcaacggctg gaagatatgg tgattgcggc
     6901 ggtcgatgag gataatatcg gcatccgcat cggccagatt cttagtagct gcaaggccgg
     6961 caaacccggc gcctataatt gcaacgcacg gacgcttgtc ttgctttgat ggttccagag
     7021 gcattgctta caaatattcg tatctttagt tattttgagg gtgaggcctt tttaacgcta
     7081 ccacccgcga ttttcacata gtcctgaaga atattgttgt tatgcgcgac ctgattttga
     7141 atgatctttg accacaaatc atactgctcc ttctggaagt tcatattttt ctccacaagg
     7201 ccggtcagaa aggcgctttg ctccttgcca aattcctgca atttcttctg ggtttcttga
     7261 aggttttcgc ccaaggcgaa gatatcgtct ttttgttgtg cactcatttt agtctttcct
     7321 ttcggttggg ggttaaaagt ttccaaaggc ctttgttaca aagcaccctt aattcgttta
     7381 taggcatttt tcagttcttc acccactatg ccgaaagcac ttgtaatatc ctgcccactt
     7441 tttgcgagat cggcgtcttt ataaaacttt tcccatttct cttccagctc atgccactca
     7501 tcctgaagat ctttcgaacc taaaaggatt ttcagccgcg tttcatcacg cagctccaaa
     7561 atctcgttaa gaagattctt atatttttta tgatccgtat cagtcatgct ttttttcctt
     7621 tataatttaa acccaatgtt tgagaaaacg gcttgccttg cgtatttaaa acaacatcaa
     7681 tattgccttt gattgcgtta gagtatggac aaacagcatg tgccgcctga accagctcct
     7741 cggcatctct ctggcttaag ccgtgaataa tcgtcgtcaa gctcactgcc agatcgaatc
     7801 cttcctgatc gttggcgtga agggaaatat ctgctatcac gctgacatct gcatccttta
     7861 tttgtatttt ctgttttttg gccaagtgaa tgactgcatt ttcaaagcat gcagcgtatc
     7921 cggctgcaaa aagttgctcg ggattagtag catcgccctt accacccatt tccttcggta
     7981 ttgctaaagc aagatcaagg agaccgtcgc tgcttttgac gtgcccgtgc cttccttctg
     8041 tggcgataac ttgtgtttta taaagggcag acattatatt ttctcctatt gcatatacag
     8101 cccgccattc acggacagcg tttcccctgt aataaagccg gattcatcgt gcgctaaaaa
     8161 tagaatagcg cgggcgatct cttccggctt tgccaaacgg cccacaggta ctttatcgat
     8221 aattttttgc agcgtttcat ccggcacggc ctgcatcata ctggtgtcgg tataacctgg
     8281 cgcaaccgca ttggctgtga tgccgtagcg cgccgtttcc agcgcaaggg atttggtgaa
     8341 tcctataatt cccgctttgg ccgccgcgta gttggtttgg ccaaactgcc cggcaagacc
     8401 attcaccgag cttaaactga tgatgcgtcc gaacttctgc tctttcattg caggtacggc
     8461 ttgacgagcc atataaaaac aggcgtttag atccacattt aaaacggatt tccattcctc
     8521 gacggtcatt ttggtgatgg tgtgatcctt gataatgccc gcattattta ccaaaatgga
     8581 gacggggcca aaatcatcgc tgattttttt aaagcccgct tcgcaggcgt caggatctgt
     8641 aacatcccat tcatagacag caataccggt tgttgcagaa aaactctgcg ctttttcctt
     8701 gttgccaata taattgcaca ctacctgata cccattttct ttgaacatca gcgctgtttt
     8761 cgcaccgatt ccggtcgttc cacctgtaat taaaactacg cgagccatcc tatttccttt
     8821 tttgtatgcg ctaagtatta cataggatag actggtcagt ccagtctatc tttgaacaat
     8881 attttacgca tgaacgctat acccgttatc aatataaatt gtctgaccgg tgatatggcg
     8941 ggatttgtct gaaaccagaa atgccgccgc ttcgccgatc tgctcgatag ataccaatgc
     9001 gtgaatgggg gcttccttcg ccgccttttc catgagagcg tcaaaatggg ataagccaga
     9061 ggccgcacgg gtcatcaccg gaccgggcga aagcgcgtta acgcggatat tccttggccc
     9121 cagctcatgc gccagataac gcatggaaga ttctaatgcg gatttgaccg gccccatcaa
     9181 attataattc tcaatgactt tttccgcgcc ataatagctc atggtcagaa tagagccgcc
     9241 atccttcata agaggttcgg cacgctttgc cagccggatt aaggaatggc aggaaatatc
     9301 catcgccatc tggaatcccg ccgaggaact gtcaagcaca cggccttgta gatcctcttt
     9361 cggcgcaaag gcaatggagt gcaggaggaa atccagcccg ccccattgtt tttttacgct
     9421 agaaaaaacg gcgtccatct gcgcctcgtc tgtcacgtcg agcggcaggt aaagcggtgc
     9481 gttgatcttg gccatgagag gctcggtata ttgccgggtt ttttcattca agtacgttac
     9541 ggccatttcc gcgcccaggg catcaaaagc ctgaatacag ccccaggcaa tagactgttc
     9601 attggcaaaa ccgacaatca ggccttttaa cggttttttg ctcattttaa acggctcccc
     9661 agccaccatc agaaggaaga ataacgccat taatattttt agcctcatcg ctggaaagcc
     9721 atgcaatcgc ttgcgcgatt tcaatagctt cagctgttgg cggcattaag gctttcatta
     9781 aaggttcgat tctattaatc gaaaggttcg attccatcgg ggcttcaatg ctggttttta
     9841 cagcgcctgg cgctacggcg ttgacacgga tgcctttatc tccatagaaa aaggcggtgt
     9901 ttttggtcag cccgttaatg gcccatttag aagccgtata agcagcacca gcaacagatc
     9961 cgcgcaaacc ggcttctgac gaaacattga cgatagatcc gtgaccggct tccagcataa
    10021 taggtaaagt tgcccgtgtt agtttcataa cagccgtcag atttacagct atcactttgt
    10081 cccatgtgga atcatcgatt tcaccgacgg ataaaaaacg atccataatg cctgcgatat
    10141 tagcaagtac gtcaattcgt tcttgagcca ttttaacgat ttcttctatc accttcccat
    10201 tgcatacatc acctgcacag gtttttatgt cgtatgcttc caatgtacgg gagagagcat
    10261 caaggcgtgc ttgtgaaata tcacaagcaa taacttttgc accttcttgt gcgaataatg
    10321 ttgcggtcgc ttccccaatt ccggagcctg cacctgtaat gacagccgtt tttcccaaga
    10381 atctttgttg tgttttttgt gtcacggatg catctccttt tgaaaatttc tccagatttc
    10441 tatcgccatt gcgcgctctt cattggccgg aatgatatga tgttcgaaag gcggcaggaa
    10501 agataaatgt cttaaaatca tgatgcggat tttctcggaa ttttctccaa tacccccggt
    10561 aaaaatcagc gcatccatac cgccgagcga agtcgccatc gccgcgatat attgcgcggt
    10621 cttcagggca aaatattcaa gtgcaaacgc ggccttttta tccgtgcttt cagacaggac
    10681 tttcacatca ttggtcaggc cggacaatcc tttgaggccg gattcattat agaggatatt
    10741 ttccgtctca tctggcgata aaccaagcgt acggattata tacagtaccg ccccggcatc
    10801 gagcgcaccg cagcgcgtgc ccatcggcag gccgtcgagc gccgtcatgc ccattgtcgt
    10861 gtcgatgctg cgtccgtttt gcatcgcaca caggctcgcg ccattgccca aatgtgcaac
    10921 caccacctta ccttcaacca agtgtggata atcggttttc aggcaatggg cgatccagtc
    10981 ataagacagg ccgtgaaagc catagcgttt taccccttgg tcacggatgg attgcggcaa
    11041 ggcaaattca ttgaataatc ggccatgccg cgcatgaaag gccgtatcga aacatcccat
    11101 ttgtggcaaa cccggctgac gactctgcaa tatatcgacg gcggccagat tatgcggctg
    11161 atgtagcggt gcgagaggtt caagactgcg cagatatttc atcgcaccct catccagttt
    11221 tacaggcgcg gcatactgtt cgccgccatg gacgatgcgg tgtgcaacca ccgaagtagg
    11281 caagcggttg tcttccagcc attttaaaac aagatcgata gcttcctcat gggacaatcc
    11341 ttcgctcaag ggatatgttt caggtactcc gttttctttt gaaacgtcaa agacaggcgt
    11401 tccgccaata ccgctgatct ttccgcccgc caataacggc aaagtttttt ccagcccaaa
    11461 caggcggaat ttaacgcttg aggaacctgt gttgagggtc agaagatggg gcattacttg
    11521 attttccctt cctgccgcgc cgccgaaagc gccatagcca caccgcagga aaacatgcgc
    11581 gtgcgcttgg aatcggcgcg gctggtcagg atgatcggca cgcgcgcacc cagaacgatt
    11641 ccggcggcgt ccgccttgcc aagaaaaata agctgcttgg cgagcgcgtt tccggcctcg
    11701 atatccggca ccaccagaat atcggcatcg cccgccacat cggaaatgat atttttatcc
    11761 ttgaccgcct gtgcgctgat cgcgttatca aaagccaaag gcccgtcgag gcggccttcc
    11821 ttgatctgtc cccggtcggc cattttgcac aaagccgcag catcgatggt agagggaatg
    11881 cgcgaggtga cttcttcggt ggcggagaga atggcgactt tcggatcgcc gtgttcatgg
    11941 ttgaacaaca catgccacgc atcaatggcg ttttggcaga tatcggcttt ttgttctaga
    12001 tttggggcaa tattgatcgc cgcatctgta atcatcagcg gcttatgata ggtggggata
    12061 ttcatcaggt atacatgaga aattcgccgt tctgtccgta atcctgccga ggatggcaca
    12121 acggcctcca ataattcttc ggtatggagc gagcctttca tgatcgcgcc cacttctccg
    12181 gccacagcca tctcaaccgc tttggcggcg gcggcatggc tgtgttcggt gttgatgatc
    12241 tgccatccct ttgcgttcac gccgctttcc ttgatcgcgg cctcgatacg ctctttcggc
    12301 ccgataagca caggaacgat aaggttctct tcaaccgctt ctgcggccgc ctctatgaca
    12361 ttggcctgta ccggatgaac aaccgcgact ttgagaatgc caaaagcgcg gcatttttcc
    12421 atcgtgcctt tgtagtggtc tttttcgcgg atatcgaccc aggggatatc cgggcgggga
    12481 attctgattt tttcggtcgg cgcgagaaca gtcgccgtgc cctcgaccag cacctcgcct
    12541 ttttcgttca ggcatatgca atcaaagaca acaacaggct tatcatcgcg ctttgatttg
    12601 acgctaacgc gtaccgtgac ctcatcgccc agcatcacag gttttttaaa gcgcatatcc
    12661 tgctccatat agattgtgcc ggggccggga agttctgttc ccagcaccgt ggaaatcagc
    12721 ccgcccgacc acatgccgtg accaataaca ccgtggaaca ggctgttctc cgcataatgt
    12781 tcatccatat gcgcagggtt tacatcaccg gaaacggctg cgaatagctc gatatccttt
    12841 tgagtgagcc tgcgcttcaa ctcggcgctt tgtccgattt caagctcttc aaatgttacg
    12901 tttgccatga tgtcttcagc catgagagcg tcctttatgc ctgatgcacg tattttcccg
    12961 gtgcatcgta aagcggtttg aatttatcat ttccaggttt tttaggcgct gcaatttctt
    13021 ctttacccat ccggttcttg agccatttat cccaggcaac ccaccatgat ccttcaaaat
    13081 ctttcgcgtt ctccagccat tcatccggcg cttcataagc gcttcccggt tttgtgcttg
    13141 ccatatgata atggcggtat ttatgccccg gttccgagac gatacccgca ttatggccgc
    13201 cattggtcaa aacaaacgtc acttcattac ccaccatcag gtggatttta taaaccgatt
    13261 tccacggtgc gacatggtct ttttcggtgc cgacagcaaa aacgggaagc gtgatgtttt
    13321 ccggtgcaat cgccttaccc agaacgctat atcttccgtt tgcgaattca ttgttgagga
    13381 aaagccgttc caggtattcg ctgtgcattt tatagggcat gcgggtagcg tcggcgttcc
    13441 aggccatgat gtcaaacagt tctccgcgtt gtcccatcag atattccttg accatgcgag
    13501 accagatcaa atcataagag cgcagcatct gaaaggcgcc cgccatttgc ttggtatcaa
    13561 gatagccttg ccctttcatc attttttcaa ggtaatccac ttcgccgggg gtcacgaaca
    13621 ccatcatttc tccggcctcg gtaaaatcac cttgcgcggc gaacaaggac aggcttttta
    13681 gacgctcatc cttttcatgt gccatataag ccgccgtaat catggccagc gttccgccta
    13741 gacaatagcc ggtcaggtgg atcggcgcat tgttcgtaat ggaggacacg gcatcaaccg
    13801 ccgtcagcgc gccgtcacgg acataatcat ccatgccgag actgcggtct tcgctggtcg
    13861 gatttttcca tgagacgata aagacctgat ggccttgctc cgtcagccat tttaccagcg
    13921 agttatgtgg cgaaagatcc agaatataat atttcatgat ccaggcgggc agtatgagaa
    13981 ctggttcctt gctcaccttc ggggtaaggg gatcatattg tatcaactcc ataagatcgt
    14041 ttttgaaaac gaccgtgcct tttgtgatcg ccagatgctc gccaggctta aagttttcta
    14101 tgcccgcagg gggctggcta ttgatattac gcttaaaatc ctcggtggcg ttcaccgcgc
    14161 cttgaattaa gcttgctccg ctgctgcgaa ccgtttcgaa taataaatca ggattggtga
    14221 gtaaaaagtt tgagggcgca cgcgcatcca gaatttgacg cgccataaac gaaacggccc
    14281 gctcagtttg aggatccagc cccggaatat cttttgtcgc cagatcccaa aactcctcca
    14341 tggcatggaa tgtttgcaaa tacatggccc acggccagat cttccagctc tccgaggaaa
    14401 agcgaggatc atcggcgcag gccggattat cgtcatcgca gccttgcaaa agacgggcgg
    14461 caagtttaac agaaggaaaa gccgccaaag acgcgagccg ccccgggcat aaggaaagct
    14521 gcgagagcca tgtcgcatac atttgcacca atccggctgg gctgaggccg taagtgaggt
    14581 gcgccagttt catctgaaaa aagtcatcca gatcatccat agcagacgta aattgctcta
    14641 taggcgtatt ggtcattttc cacatatccc atgttttgtt gtgcactgtg ccataattag
    14701 actggactgt ctagtcgcaa tatgctagaa gtggacgaat tttcaggatg gacaccatgc
    14761 agataaggca acaaagggaa gatatgtcga aagaaagcgc tattttagaa gcggcaacac
    14821 attcgttcct gcgtaaaggg tttagtggtg taagtatgga agagattgca cgcgaatcta
    14881 atgtcgccaa acaaacgctt tatagctatt tcaaaggcaa ggatgattta tttcgtgcca
    14941 ttattcatgc acaaagcgac gcgttttttg ctactttgcc tcacaatgat gaccaaactc
    15001 agaatataga agaattcctg cacattatgg gagaaagagc gatggatatg ctcttcaaac
    15061 gcgacacact agcgctgtac cgcgtgatcg tatctgaaac accacgtttc ccagaacttg
    15121 ggaagttcta ctggcaatca ggggcgcagg cttttaccga ccgtctcagt cattttttaa
    15181 tcgctcgcgg gtttcccaag ggaaaggcgc agagcgccac agaacagttc ttaagtttta
    15241 tgtttggcac attgctgaca aaaacgctgt taaataaaga atatcatcct tcgaaggaag
    15301 agaaaaaagc gcatatcgag caggccgttt ccgcgttttt tatgctgaac ggtcaatatg
    15361 taacagaaaa agtaaatcca tgaaaaagaa aaacacattc atacgcctta ccattatact
    15421 cgcgcttgca gcgatcctcg gaggcggcta ttttttctgg caagcgcagc aatcaagtct
    15481 gccggaattt atagcttcgg gtaatggtcg tattgaggca gaagaaacac atgtcgcggc
    15541 caaatatgca ggccgcgtga tcgacgttct cttcgatgaa ggcgatatgg tcaagcaagg
    15601 cgacattatc gcacatattg acgcagaaga tctggaggca caacatgacg gcgcaatagc
    15661 caatctttcc tctgcaaaaa atgccgtagc agaagcgcag gccggagtta ttcaccgtga
    15721 ggccgagatg gaactggccc aggccgaaga gcagcgcgcc aaggccttgg tcggcaccgg
    15781 ggtttccaaa gaactgtatg atcaacgcaa ggcctcggtt aaaacggcag aagccgccct
    15841 gaatgttgcc aaggcacaac tggcttcggc agaaaatcag gtggaaacgg cgcaatccga
    15901 aatcgaccgt ttgaatgtct tgatcaaaga cgcgacatta aaagccccta tgagtggacg
    15961 cgtgcaatat cgtctggctg agccgggcga ggttttgagc gccggatcac gtgttgtcac
    16021 catccttgat cttgagaatg tctacatgac catttttctg cccgccaaag atatcggcaa
    16081 aatcattgtt ggcagcgagg cgcggatcgt gctggacgcc ctgccggatg tagtcattcc
    16141 tgccaaagtc tcctttattt cctctgaggc gcaatttacg ccgcgcgaag tagaaacaag
    16201 agaagaacgt gaaaagctca tgttccgcgt aaaaatccgt atccctaaat ctttgctttc
    16261 taaacatatt gagaaagtcc gtactggatt gccgggcatc ggctatgtca tgctggggga
    16321 cacaaatgac tggccggagc atttaaagat gaatatcgga caatcacagg agtaaggtgt
    16381 gaacatgcaa gagcgcagcg ttatcactct ttcgcatgtg acacatgtct acggtaaagc
    16441 gcaggcgctt gatgatgttt ctctggagat ccctgctgga aaaatgatcg gttttatcgg
    16501 ccctgacggt gtcgggaaat cgaccttatt cggccttgtt gcgggcgcac gaaaaattca
    16561 gagcggcgaa ataaaggttt taggacagga tatcggcaac gataaaaaac gcaaagacct
    16621 tcttccccgt attgcctata tgccgcaggg attaggcaag aacctctata tggatttgac
    16681 ggtattcgaa aatctggatt ttttcgcccg tctgttcggt caaggtaagg ctgagcggga
    16741 agcgcggatt aaaacattgc tcaaagccac agggcttgat ccgttccccg atagacccgc
    16801 cgggaagctt tcgggcggca tgaaacaaaa gctggggctg tgctgttccc tgattcatga
    16861 tcctgacctg ctgattcttg atgagccgac aacgggcgtt gatccgctct cccgccgtca
    16921 gttctgggat ttgatccgct ctatccgtaa agatcgcccg cagatgagtg ttctgatcgc
    16981 caccgcctat atggaagaag cggaaggctt tgactggctg gctgccatga atgacgggaa
    17041 aattcttgac accggcacgg ctgaagaatt gaaagacagg acgggtggcg gaacgttgga
    17101 agaagccttt atcgctctgg tcgatgatgg gaaacaggtc aagcatgaaa aactggttct
    17161 accgccttat gaagatggtc atggagaaga tccggttatt gtgacgaaaa acctgactaa
    17221 aaaatttggc gatttcacgg ctgtcaataa tgtctcggtt gagatcatgc cgggggagat
    17281 tttcggcttt ttaggctcga acggctgcgg caaaacgaca accatgaaaa tgctgaccgg
    17341 cctgttgccc gcaagcgagg gcgaggccaa aattttcggt aaaacaccca gcaatgacga
    17401 catggaattt cgccaccgtg tcggctatat gtcccaggcc ttttcgctct attcagaatt
    17461 gaccgtgtgg cagaatctgg agctgcatgc ccggcttttt catgtgccgg acgatacaat
    17521 caaaccgcgc attgatgaat tgctgagacg ttttggcctg gagacgcata aagacacact
    17581 gccatcttca ttgccgcttg gcgttcgtca acgcctgtcg ctggccgtgg cggttgtgca
    17641 tgagccggaa atgcttattc tggacgagcc aacctccggc gtcgatccgg tcgcccgcga
    17701 cgagttttgg aaactgctga tcgatctgtc ccgcaataaa aaaaccacga tttttatctc
    17761 gacccatttt atgaatgagg ggatgcgctg tgaccggatt acgctcatgc atgcgggaaa
    17821 tattcttgcg tgcgacgcac cggacaaact gatcgagggt aaaggcaagg acacgctgga
    17881 agaggccttt atcgcctata tggaggaagc caatgcagaa aagggcggaa aacctgacag
    17941 cagcgctgcc agcgacataa cggcaagcgt tgcagatgat aaaaagaccg gcgcttcact
    18001 tgctcttgtt cagggggggc ggcgcattgc cgcctatgcg atgcgcgaag ccaaggaatt
    18061 atcacgcgac cgcatccgcg aggctttcgc tttcttcggc acgatgatcc ttatgctggt
    18121 ctttggtttt ggcattacgt tcgatgttga aaatattacc tttgccgttc tagatcagga
    18181 taagacgccg gaaagtcgcc tatatacaca agctctcgaa ggctcacgtt atttcacgga
    18241 aaaagcgccg cttatcgatc atgccgatat ggatcaccgc ctgaagagcg ggcaaattac
    18301 gctggcgctt gatattccgc ccggttttgg caaaaacctg ctgcggcaaa aatcgccgga
    18361 agtcgcggtc tgggtggatg gcgccatgcc gttccgggcg gagacggcgg gtggttacat
    18421 gcaggggctg cattatgatt ttatcggcag gctctatgaa gattattacg gtgaacaggt
    18481 tgcactttct cccgtctcga ttgaaccgcg ttttctctat aatcagggtt ttaaaagcgc
    18541 ctatgcgatt gtaccgtccg tgattgccct gatgctgctg tttattccct cgattctggc
    18601 ggctttaagc gtcgtacggg aaaaggagct gggatcaatc accaatctgt atgtcacgcc
    18661 gacctcaaag tttgagtttt tactgggcaa gcaaattccg tatgtcgcca ttacgatgat
    18721 gaatttcctt ttgctggttt taacggcctg gctggttttt ggcgttcccg tgaagggatc
    18781 ctttattgcc ctgtttatcg gtgcgttcct ttatgcaggt tgtacaacgg ctttgggact
    18841 gttcttttct gcctttacag ggacgcagat cgcggcgctc gcggtgacgg ccattatgac
    18901 cttgctgcct gccgtacagt tttccggctt gatgcagcct gtgtcaacgt tggaaggcgt
    18961 gggtgccttt atcgggcgca tttacccgac cacacatttt attacagtgg ccaaaggcgc
    19021 cttcgccaag gatctggatt ttgctgaatt atggcccagc ttgcgcgctt tgctgatctc
    19081 ctggccgatc attctgaccg ccggaatatt ctttttaaaa aagcaggagg cgtaagcaat
    19141 gcggctgagt gtaaaaaata tataccgtct tggcattaaa gaactgcgca gcctctaccg
    19201 cgatccggtg atgctgttta tgatactctg ggcgttcagt gcatctatct atatcgctgg
    19261 aacatcgatt tcccacgacc ttcacaatgc atcaatcgcc attgtagatg aggatcaatc
    19321 gcctttgtcc ctgcggatcc gcagcgcatt cctgccgccc tatttcaaac agccggacat
    19381 catcgccttt caggacatcg atgaaggtat ggatctggac aaatacagtt ttgttctggt
    19441 tattcccgaa gagttcgagg ctgatgtgtt gcgtagccgc cagccaaccc ttcaggtcaa
    19501 tctggatgcc accgccgtta tgatcgccgg gatcggatcg ggctatattc aaaatatcat
    19561 aaatgatgag ataacgcgtt ttacaaataa cggcccgtcc acatcgcaaa gtccggtcga
    19621 tatgaggctt cgttatgcct ttaatccgaa tctgtcatcc aaatggttta cgagtattat
    19681 ggaggtcatc tccaacatca cgatgtttgt tattctgctc accggtgccg cgctgatccg
    19741 ggagcgcgaa catggcacaa tcgaacacct tctggtcatg ccggtgacgc cgattgagat
    19801 tatgctctcc aaagtttggg ccaatggtct ggtgatcctc acggcaacag cattttgttt
    19861 gtgggtgatg attgagggcg ttttgcaagt accgattgcc ggatcggtgc cacttttttt
    19921 gttgggaacc ttgctttatc tgttttttgc ctcggccttg ggaatttttc tggggacgac
    19981 tgcgcgttcc atgccgcaat tcggcctgct gtttattatt acgatcctgc cgatgaatct
    20041 gctttcgggc gggcaaacgc ctctggaaag ccagccggac tggctgcagc acatcatgtg
    20101 gttcgtgcca tcaacccatt ttgtatcctt cgcgcaggcg attttgtatc gcggggcggg
    20161 gcttgatatt gtctggccga attttctggc cgtgagcgtg gtgggcttat cactgttaac
    20221 attatctgct ctggcttttc gccgatcaat tgcggcttaa gttggagaaa gcacctcgat
    20281 aagattttag aatgtgtcta atgaaaaaaa tgttacagtt gcccagcata aaaggagaaa
    20341 atactatgaa aagaaaagcc atacttatga caggcctgat agcgataatg ctgatgagcg
    20401 gttgcgcggg cggtatgcgt caagacggcg tcaagcaaaa agtgcaagat gaatttgtct
    20461 ttgaaaatat cgataagaat gccgatggaa atattgaccc tcaggaattt gctgcattta
    20521 agcaaaacct gaaatcctta aaacgctact agaatttctc agtcgtcagc tttcgcaatc
    20581 taaaacctgt accctaaaga gatcgcgccg aagagagacg ggtcgtcttg cccatcaaat
    20641 tcctttgacc gccagttgat ggtataggaa aactgcgctt tgccgtaggt aaagctcacg
    20701 cccgcgttgg catcagccac caggtatttt ttatcaaccg aggggctgtc ctcgaacgta
    20761 ttgccgtcca gaaatatatt gcgtcccatg gcgcgcccct ccactccggc aaacaatgac
    20821 caagcgaaac gatcctccgg cacggcaaaa aagccgctac ccggaatggc agggcgtacc
    20881 cgcaaagggg tagattgcca tttatactgg ctgggcgtga gttgaaaact caaaccaccg
    20941 gagccgtatg tatatatatt gccaagcgta atgccagcat gaggtgccgc ccgaaatgtg
    21001 aagccatcta tatcggctgc ataggcctcc ggccattggc gctgccagga gagcatcagt
    21061 cccggctcat tctccagttg gtgatcccag cccgaagggt cgtctgcatc tagaacgtca
    21121 tgaacaaatt tttgagtctg ttcccccaaa gcccaggggc cgacgatccc gatcgtggct
    21181 tccaggtcat ccacgtgatt atccgtcaaa ctggtcagtc cggcagaaac atacaaaaac
    21241 gcagcataag gacgatcttt cggatcgggc gtacgcaccg tgatattttc tggtgtatac
    21301 aggttttgtc cgaaagaata ataggttgaa gtcgtttcat taatttcaaa cgtggggaca
    21361 tagtgatcca gtatgtcggc aaaccacggt ggatcggccc cataatcgaa ataagtaagg
    21421 cgtaccccgc ttgtatagtt ctcatcggtg ttcccgccaa agttatcatt ctcaatcgtg
    21481 aatgtaaaaa aatcagcatc ttcgttaaga agatctttag gaattctatc tttaaaatta
    21541 ttgtcctgct cgttggcata agcatgtgac acaagaaggg agtagaatgt tatgagggca
    21601 agagatgtta taaaaaaaag aaatttccgc atattcatcc cttttccaat cctaaattgg
    21661 aaaaaaacaa agtgatcaat ttaggcgctt tgttttcaat atcaaagcct tccgggttat
    21721 ccagatattc aaaaaggatg cctttcataa agcacgaaat acactgtgtt aaaagttcgg
    21781 gatcggcctg tgtatttaat tttccggctg ttttggcttt ttcgatgtaa cgtttaaaca
    21841 ggcgcatgct ctccgctttt ttctcgcggt gtttgtcttt atagggtgca agatcgccgg
    21901 aataatcgct tttcatgaaa aacagaataa gggtttgctg tctttgttta tctttttcga
    21961 tatccaacat gatctggata cataaatcac gaagctgcac catcggttct ggatggtctt
    22021 tttccaggtc ttccaggatc atgtcaacaa aagggcgatg caggc
//
LOCUS       DAOSWR010000015        21298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1572_length_21298_cov_6.078755, whole genome shotgun sequence.
ACCESSION   DAOSWR010000015 DAOSWR010000000
VERSION     DAOSWR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 21298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21298
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1572_length_21298_cov_6.078755"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..449)
                     /locus_tag="PK513_02175"
     CDS             complement(285..449)
                     /locus_tag="PK513_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81292.1"
                     /translation="MHKFSALLFWGRIKVLFALCEEGLGGFEEFVVKGEGGAQGAPIA
                     VVETVFGGVL"
     gene            complement(580..1938)
                     /locus_tag="PK513_02180"
     CDS             complement(580..1938)
                     /locus_tag="PK513_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOO81293.1"
                     /translation="MSQEKPLVEVGRSESEEVSTDGMAARYAGLMLLEDVLQRKQALD
                     QALDQQQAFKALPMRDRAFTRMMVSTILRRLGQIDNIIERAIEREVPRNITVQNVLRI
                     GVVQILFMDVADHAAVDTSVKLAEKLGMEKQKAFVNAVLRTVTREGVHWRELQDEVRL
                     NTPEWLLKIWIEDYGLRMAAEIAKANLSEAALDISVRDRESRNFWASNFKATQMTTGT
                     LRKNSGGAVHDLQGFDEGHWWVQDASAAIPAQLFGDVDGQHVYDLCAAPGGKALQLAA
                     MGAQVTALDRSANRLKKLKQNLERMQLSDRVEVITADAGSWRPKGEAGYVLLDAPCSA
                     TGTIRRHPDALHLKVPQDIERLSHLQEALLENAFDMLMVGGVLVYCTCSLQKAEGERQ
                     IERFLVAHQDAQRLPITARELGGFDEALTEDGDLRILPFHQAALGGMDGFYIARLTKV
                     AP"
     gene            complement(1938..2249)
                     /locus_tag="PK513_02185"
     CDS             complement(1938..2249)
                     /locus_tag="PK513_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOO81294.1"
                     /translation="MNQDTPPELEVFRQQIDECDQELIDVLARRFNVVRQVGAFKVGA
                     QMEAIQSAREQAVKDRAVRLGQELGLDSDFIRRLYEVMIDYAHDIEHEILAQDAQDKG
                     E"
     gene            2295..2477
                     /locus_tag="PK513_02190"
     CDS             2295..2477
                     /locus_tag="PK513_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HOO81295.1"
                     /translation="MNDKKPTPETQAAAKKNKAAEQHKKPSAKTEKEVGGFADSGLLE
                     PTRFGDWEVKGRCSDF"
     gene            2604..3209
                     /locus_tag="PK513_02195"
     CDS             2604..3209
                     /locus_tag="PK513_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HOO81296.1"
                     /translation="MPIVVELFTSKFCPACPNADHNFNQLIAKDPNIIGLSCHVSYFN
                     RNGRGDKLARPFCDARQNVYKLALNTGGIYTPMTIINGSAVTTGIKQNELNPLIQKTK
                     TAKNRPVGFLRNGEYLNISLPQINLPHGADIWLFEIENRPDEENYTHYRNTVKNITKL
                     LRWDGKKLNMAFPAEQKAGISYALIIQTYKGGIIAAAQTGS"
     gene            3212..3607
                     /locus_tag="PK513_02200"
     CDS             3212..3607
                     /locus_tag="PK513_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO81297.1"
                     /translation="MAKKEKHKNRQTLEEILQSISGIMFPAEPEKHIDINCHGYCGDT
                     PLHVMLWNNNNHGAELLIEAGANVNTKGEMDETPLHVALRQKNTKLIKLLLQKGAKDN
                     IKSEFDETPREMARFMDKDIQKLFQFKNL"
     gene            complement(3618..4418)
                     /locus_tag="PK513_02205"
     CDS             complement(3618..4418)
                     /locus_tag="PK513_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOO81298.1"
                     /translation="MRLVTWNINSVRLRLPLVKRLTEEAAPDILCLQETKCPDEYFPT
                     GDLRAMGYEHQHFHGMKGYNGVAILSRLPMDARDVHHRVGREDCRHIAASISTPSGGF
                     ELHNLYIPAGGDEPDPNVNDKFAHKLDFVDEMTDWFKDRYSADQKLVAVGDFNIAPYE
                     HDVWSHKQLLNVVSHTPPETDRLAGMLGSLDWVDVARRFVAMDEKCYTWWSYRNRDWK
                     KSNRGRRLDHVWVTPPLRESLEAHQVFSKARDWEKPSDHAPVIVDIEL"
     gene            complement(4429..5193)
                     /locus_tag="PK513_02210"
     CDS             complement(4429..5193)
                     /locus_tag="PK513_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO81299.1"
                     /translation="MTGYIPKIVFITGATGDFGRAFARRFAGVGSALILHGRSQEKLD
                     ALAEEFPYVRAHTLCFDVTDQAAIAEAIDSIPEAFGAIDLLINNAGGALGQSPADEST
                     LDDWLKMIEINNSALVAVTHPVLQGMSVRRRGHIINIGSTAGNYPYPGGHVYCAVKAF
                     VKQFSLALRADTYSKNIRVTNIEPGMVKTQFSLVRYKGDQKAADAVYANTTPLTADDV
                     AESVFWAASQPSHVNINRIELMPTRQAPGPLAVDRD"
     gene            complement(5196..5516)
                     /locus_tag="PK513_02215"
     CDS             complement(5196..5516)
                     /locus_tag="PK513_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOO81300.1"
                     /translation="MNFKDMMQQAQQMQFKLEELQEKMKDIEVEGEAGGGMVKVTMSC
                     GGEVRAIAIDSSVINVDDKETLEDLIVAAMNNANDAKDERIKTETTGMMEGLGLPAGT
                     KLPF"
     gene            complement(5516..7288)
                     /locus_tag="PK513_02220"
     CDS             complement(5516..7288)
                     /locus_tag="PK513_02220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOO81301.1"
                     /translation="MSEAQVPYRVLARKYRPQSFDELIGQDALVRTLTNAIESGRIAH
                     AFMLTGVRGVGKTTTARIIAKALNYVGADGKAGPTTGATEDCKICQAIAEDRHPDVIE
                     MDAASRTGVDDIREILDGVRYAPSEARYKVYVIDEVHMLSKNAFNALLKTLEEPPEHV
                     KFIFATTEIRKVPITVLSRCQRFDLRRVDVPTLSAHFTSICEKESVKAEPEAIAMIAR
                     AADGSVRDGLSILDQAMALNGESITSQAVEDMLGLADRARSLDLLERALSGDMPEALE
                     IMDDLYRAGADPVALIQDLLDLTHTLTKLRAVPEAKEMSHAMAADEVKRAAELAGKLS
                     MPTLGKTWQILLKGLAEVQAAPNPQSAAEIVLIRLAYAADLPDPAALIKTLQGQESGP
                     VTSNAGGISQSASAPVGAPVSNASSPSQSLNAGARTGIPTGRSFAGPPSGKGSGGERA
                     ALAVVPKVEPPSPLEIRNLEDIVWVLEEHREVLLASQVYQYAHLVKLEKGLIEIRPEA
                     EASPKMAQDLGQALSRISEARWIVSVSGAPGEPTLAQVAGARLEAERAEIIKLPIMQE
                     ILSVFPDAEIQSIEMVNNKGHDKP"
     gene            complement(7359..7457)
                     /gene="ffs"
                     /locus_tag="PK513_02225"
     ncRNA           complement(7359..7457)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK513_02225"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7555..8658
                     /locus_tag="PK513_02230"
     CDS             7555..8658
                     /locus_tag="PK513_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOO81302.1"
                     /translation="MKNLVSLLTRLADADMVFWLLPPLMALLVSGTLAQRWMGLWPAM
                     EMFFSSFIIWAGPVPLPGAYTLLGILSINLTLKFLLKSRWQWEKSGIILSHLGAIILL
                     LGGLLTAMTAREYYMLIPEGQESPFIYEYHRRALNIFENDRQIARLPFENIENWDLSA
                     LPFQIRIQNSCQNCDIQKRQEQPGYDPEQPWQGMARFMVFDPKPNEKDPESDLSGLQF
                     EVTGTDQDGIYVAFDAMPKPIEITKGEAIYRLMFGKRQSILPFTIALTDFVKNEYGGT
                     AMARSYHSDIIIKDGSLEWPARIEMNKPLRYRGYTFFQSSFEQGPDFEATILAVVENK
                     GRLFPYIGSIVLGLGLILHMGLILIRKNTRSKA"
     gene            8655..9194
                     /locus_tag="PK513_02235"
     CDS             8655..9194
                     /locus_tag="PK513_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81303.1"
                     /translation="MRIFLILASIITALLLPQNAGAEAHFEMQYFKTLPILHEGREKP
                     LSSFADIMLRQFSGQEKLQNMDASQWLTLTLFDPQSAAELPVFTVSDETLITKLKLDK
                     TQNLYSYAQIQPALKAMRDEALPLFSKEETALTGQEKTLLRLYENTALFTALLRSFTA
                     LLPLDLSLPPPIKTKSMEH"
     gene            9296..10519
                     /gene="ccsA"
                     /locus_tag="PK513_02240"
     CDS             9296..10519
                     /gene="ccsA"
                     /locus_tag="PK513_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOO81304.1"
                     /translation="MTIAKASFHLQTLRAGAQDNELLRIIPVQWEDSKDQWATPWTIM
                     LQGQGGPGAAFLLSQWTDLAGAYRQNDARRWKTISEDILEETLLQSPQSLNIKRLKIE
                     QLYRTVHPYTLIITLYGLSIFAATFLLFKQPTARLLRLAPTLLALTGIVLHIVTLTAR
                     IYILQRPPVGTLYESILFVTLICAALGILLQRARTSFIPLITGTGTAAALLICAPVFK
                     PDGDSLEVLAAVLNTNFWLTVHVLCITAGYGVCILAAMLAHSGLYISGFKHNKELSKK
                     LRQNVHRTSIAALLLTAIGTALGGIWADQSWGRFWGWDPKENGALLIVLWLVWVQHGR
                     ISKHLSSTGFMAMSAALNIIIAISWFGVNLLNVGLHSYGFTSGLAGSLIAFCIAEIAL
                     ITFLYIAAKKQECAA"
     gene            10516..11088
                     /locus_tag="PK513_02245"
     CDS             10516..11088
                     /locus_tag="PK513_02245"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO81305.1"
                     /translation="MKITPLINFAVLVFIGCAAYLATLYLDRQAPDIKTAPESVLKQT
                     ENNQSVPNFSFTGTDGKTLSIKDFQGKVIILNFWASWCTPCIKELPLFLKAANENPKD
                     LIFIGLSSDLEKEKMIRFLKKQKFDISQKNIFFALDSENITQSLFQTFRLPETILIDK
                     NQIMRTKIIGANWTYEELISKIKAAQTLQQ"
     gene            11203..12762
                     /locus_tag="PK513_02250"
     CDS             11203..12762
                     /locus_tag="PK513_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO81306.1"
                     /translation="MQNAATNLVPFMSSKPEAVTILNMLPVNVMTCNPNTFVIDYANK
                     RSIETLNSLQHLLPAGISGDNIVGQCIDIFHKTPQRQRKMLANPSNLPHQAIIRLGPE
                     MLDLHVDAIMSGNKIKKLLLSWSVCTERERLKIMIDNMPINIMMCDPQSLKLTFLNKT
                     SIETLRSIEYALPVKADNMMGACIDVFHKNPQMQRQLLGDPNNLPHKAKIRVGDDVLD
                     LNVAAILDDSGHYIGPMVSWAVITAQENLSKSVLEVSESVSASSNDVQTTAQALSAAA
                     EESSAQATSVAAASEEASTNVQTVAAATEEMTASIKEISAQIAKSNETAGEAVKKAEE
                     TNSTVEALHEASNQIGEVVNLINDIAEQTNLLALNATIEAARAGEAGKGFAVVASEVK
                     SLAAQTAKATEEIRTQIDKMQSTTSDAVTAIGSIRTTISAISDATSSIAAAIEEQTAT
                     TSEISRNVQEASRATAEVTSNISGVQQAASETGSASTQLLELATQLSERSNEMNKQVN
                     DFMNAGQKSKK"
     gene            12953..17752
                     /locus_tag="PK513_02255"
     CDS             12953..17752
                     /locus_tag="PK513_02255"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOO81307.1"
                     /translation="MSKSLIKEALKALPGKPSANKTELLEKLAQSMTQEDQDHFNHTL
                     LAKIVEIHEKLAEKRENGDPQLKIYTVTDSHLGPRKTIIDVVSDDKAFLVDSVVAEVN
                     KNSFLIDLLLHPIIYAKYSEDGKLLKTSPTEKEGFIRQSHIHIQIKDTLPESAVKALE
                     IGLYEAVQDVHIANKDWREMLEKLKDARQALENAKTRRPLKEVQQYCAFLDYLHNNNF
                     TLLGYREYKFVEKNGEVTSRTVPSSGLGLLHKEVRPAYINDHDEGLPRNLQEKRRNLP
                     PLSISKTNRLSTVHRRVPMDAIAIKTYDEDGTITGEKLFIGLFTSVTYSRSVGDVPYI
                     REKVEEVMEMAGYSIGSHDRKALRHILEKYPRDELFQISTKELLKTTTSILKLQERQR
                     IALFMRKDPFRRYVSCLVYIPRDRFATSLRKTMCSILEKQLNGTCDTFYTSLDDSVFA
                     RVMFIINIRQTDDPMLDVKRIEKMLQEAGQTWQELLAQALSEVYANENNITATCQKYG
                     EAFPVAYTSRYRPKQALFDIDKIEQVLDTKNLQLDLYRPDDIDPKQLRLKVYNPQRPL
                     TLSDVMPILENLGLRAISELPFEVRPYKEKQSIWIHDFLLETPEMDEIVDVASVKQNF
                     ERGFSKIWSSEMENDGLNRLILAANMNWHEITILRTYIRYMKQIRYPLSRPYTEKALT
                     VHANISRMLVTLFKSLHDPKHQKTSAKKARECMANIENALEKVESLDHDRILRTISKL
                     IEATLRTNYYQRLDNGRAKSYLSLKIESAKVPEMPAPRPYREIFVYSPRVEGIHLRGD
                     KIARGGLRWSDRHEDFRTEVLGLMKAQMVKNAVIVPMGSKGGFVVKQPTHSREEFMKE
                     GIECYKTFIRGLLDLTDNRKGNLIIPPKDVLRRDDDDPYLVVAADKGTASFSDIANGI
                     AREYGFWLDDAFASGGSAGYDHKKMGITARGAWESVKLHFRQLNHDTQTQDFDVVGVG
                     DMGGDVFGNGMLLSKHIRLIGAFNHLHIFCDPNPDPATSWDERKRLFDGVKGWGDYDT
                     KKLSAGGRIFERSEKTLTLTPEIQKRFDISKEKVTPGELMRAMLKARTDLLWFGGIGT
                     YIKSSKESHADVGDKASDALRINAPDLRAKVIGEGANLGVTQLGRIEFCENGGKMNTD
                     FIDNSGGVNSSDLEVNIKILMTDVMGGKTHNMDIKARNKLLEKMTDEVAGLVLRNNYQ
                     QAQAISLAELQARENLQIQDEFMKDLEREKGLNRALEGLPEEEKIEQRLRAGKGLTRP
                     ELAVLVSYAKISFTQDLMASDIPESVDMEDWIINYFPGDLRKKYIKEIKRHRLAREIV
                     ATSMANSLVNRMGPTFLKEKMNKTGSSAAEIAKAYIIVRDAYNLRALWDQVEALDNKV
                     PAEVQLKAMREMALLSEHSISWFLTRLGHDLDIGREIKDFGEGIKNLSSNLSKLLTND
                     LKASVNMRIAAGQRDGLPKALAEQIALMPVLSSACDIIRIALEQKTDLLKTARTYFEV
                     GEEFHMDWLRQQARYLPSDDHWQAEAVRGLVDQLFGAQAALTVRILIDTKDKTNKGKT
                     PAETWMDDHAHKVMQLTPLFADLRRAGTIELPMLIIAEQRLRNLAGC"
     gene            complement(17749..18654)
                     /gene="ubiA"
                     /locus_tag="PK513_02260"
     CDS             complement(17749..18654)
                     /gene="ubiA"
                     /locus_tag="PK513_02260"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOO81308.1"
                     /translation="MFTDIRLWNWIEKLPERFRPYVYLARLDRPIGIWLLLLPALWGI
                     TLAASHLSGFSFKVLWLIFLFALGASVMRAAGCVVNDIWDRKLDAQVDRTRVRPLASG
                     EVSVQQALIFLFGLLMAGFCILILMNGITILLGLLAVPLIVIYPLMKRVTFWPQAVLG
                     LTFNFGALMGWAAVMDGLSAQALLLYVAACLWTLGYDTIYAHQDKEDDALIGVKSTAL
                     KFGENSKKWVSGFYAVMLVFLGWGFALSSLNAFALPGLALIGMHLFWQIGRWDMDDPA
                     CSLAVFKSNRDLGLLVLAVIFAGGF"
     gene            18748..19458
                     /locus_tag="PK513_02265"
     CDS             18748..19458
                     /locus_tag="PK513_02265"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOO81309.1"
                     /translation="MKDTHKTPRLYIDQPLNAGKDVILETPQAHYLKSVLRFEKGTPV
                     RVFNGRDGEWLATISALEKKSAVLTLTNQLARQPVQPQPLHLLFAPIKKHRMDFLIEK
                     AVELGVTDLTPVITAHTQTRKINEERLHAQIIEAAEQCERMSLPTLHKVETLPGALQK
                     WNQQIPVYWAAERLKNTPHIKEIISPEAFLIGPEGGFSEQESDFLQTQEIVRPISLGK
                     NILRAETAALFCLSHANP"
     gene            19611..20450
                     /gene="coxB"
                     /locus_tag="PK513_02270"
     CDS             19611..20450
                     /gene="coxB"
                     /locus_tag="PK513_02270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOO81310.1"
                     /translation="MIKRFLTFALAVFVSFPGAPAFAGQPMPWQIGLQEPASPSALRI
                     FEFHDMLLYIIFSIAIFVLLLLVWVIIRYNAKANPQPAQFSHNVLIEVLWTVIPIVIL
                     IIIAIPSFKVLYYNDRVENPDMTLKVTGYQWYWTYEYPDHDGLNFSSYMIADKDIDES
                     KDQKRLLSTDNVVVLPIDTNIQILITAGDVIHSWAVPALGIKLDGIPGRLNETWVRIE
                     KEGTYYGQCSELCGKDHSYMPIEIHAVSKDEFKNWLITAKKEFASLDASDDSLIRMAG
                     FQK"
     gene            20494..>21298
                     /locus_tag="PK513_02275"
     CDS             20494..>21298
                     /locus_tag="PK513_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOO81311.1"
                     /translation="MADHKPGFFARWFMSTNHKDIGTLYIIFSIFAGIVGGLFSMIMR
                     MELQDPGVQFMVDSAGNPDGHMWNVFITAHGLIMVFFVVMPGLIGGFGNWFVPLMIGA
                     PDMAFPRLNNISFWLIIPAFLLLLSSAVIGGGAGTGWTVYPPLSSLLGHPNLAVDMAI
                     FSLHLAGASSILGAANFITTIFNMRAPGMTLHKMPLFCWGMLVTAFLLVLAVPVLGGA
                     ITMLLTDRNFGTSFFNPEGGGDPLLYQHLFWFFGHPEVYIMILPAFGIIS"
BASE COUNT         6025 a         6246 c         4364 g         4663 t
ORIGIN      
        1 ctcctttttt gtctccatgc atcaggccgg gctgggcaaa aaatcctctc tctcgcccga
       61 ggccttaaac atggctctgc aaagccaaag actttgccgc caaaccttca gcactctcaa
      121 agaatcgcaa aaatctgaaa acaaactaaa gaacgatgaa aaatcaaaat aaaacaaaag
      181 gatggccacc tgaaagacgc gccacccaag ccgcgcgcat ccgcgcgcac cgaccctgga
      241 ccaaatccac cggccccaga accccacaag gcaaagcccg ttcatcacaa aacgccacca
      301 aacacggttt ccacaaccgc gattggcgcg ccctgcgcgc cgccctcacc ctttaccaca
      361 aattcctcaa aaccgcccaa accttcctcg caaagagcaa acagaacttt tatcctgccc
      421 caaaacaaaa gcgcagagaa tttgtgcaag agctaaaaac attctcaaag agcgaacaga
      481 atttttgccc tgacaaggca gccgaagcga ggtgcgcgga acgtacacac cagtacgtga
      541 gcacaccgag actgaaggat aacgcagtca gggtgaaaat tatggagcga ccttcgttaa
      601 ccgcgcaatg taaaacccat ccatcccgcc aagcgcagcc tgatggaacg gcaagatccg
      661 caaatctccg tcctcggtga gagcctcatc aaacccgcca agctcgcgcg ccgttattgg
      721 caaacgctgc gcatcttgat gtgcaaccaa aaatctttca atctgacgct caccttccgc
      781 cttttgcaaa gaacacgtac aataaacaag tacgccgcca accatcagca tgtcaaaggc
      841 attctccaac aacgcttctt gtaaatgtga aagccgctca atatcctgcg gcaccttcaa
      901 atgcaaagca tccggatgac ggcggatcgt ccccgtcgca ctgcacggcg catccagcaa
      961 aacatacccg gcctcgcctt tcggccgcca actccctgca tcggccgtaa tgacctccac
     1021 ccgatccgaa agctgcatcc gctccagatt ttgcttcaat ttcttcaacc tgttagcact
     1081 gcgatccagt gccgtaacct gcgctcccat ggccgcaagc tgcaaagcct tgccccccgg
     1141 cgccgcgcac aaatcataca catgctgccc gtcgacatca ccaaaaagct gcgccggaat
     1201 cgctgcgctt gcatcttgca cccaccaatg tccttcatca aaaccctgta aatcatgcac
     1261 agcgccgccc gaattcttgc gcagcgtacc cgtcgtcatt tgggtagcct taaaattact
     1321 cgcccagaag ttgcgcgact cgcggtcccg cacggaaata tccagcgccg cttcagacaa
     1381 attcgccttt gcaatctccg ccgccatacg caagccataa tcctcaatcc agattttcaa
     1441 aagccattcc ggcgtattca gccgcacttc atcttgaagt tcgcgccaat gcacaccttc
     1501 acgcgtcacc gtacgaagca cagcgttcac aaatgctttt tgcttctcca tacccagctt
     1561 ttccgccagc ttaacagacg tatcaaccgc cgcatgatcc gccacatcca taaacaaaat
     1621 ctggaccacg ccaatgcgca aaacattttg cacagtaata ttacgcggca cttcacgctc
     1681 aatcgcgcgc tcaataatgt tatcaatttg ccccaaacgc cgaagaatcg tcgaaaccat
     1741 catacgggta aaagcccggt cacgcatcgg caacgcctta aatgcctgct gctgatccag
     1801 cgcttgatcc aacgcctgct tgcgctgcaa tacgtcttcc aacagcatca gccccgcata
     1861 ccgcgcagcc ataccgtcag tggacacctc ttcactttca gaccgaccaa cctcaacaag
     1921 cggtttttcc tgactcatca ctcgccctta tcctgcgcat cttgcgccaa aatctcatgt
     1981 tcaatatcat gtgcataatc aatcatcacc tcataaagcc tgcgtataaa atcggaatcc
     2041 agacccagct cctgaccgag cctcaccgcc cgatccttca cagcctgctc ccgcgccgat
     2101 tgaatcgcct ccatctgcgc accaacctta aacgcaccaa cctgacgcac cacgttaaaa
     2161 cgccgcgcca aaacatcaat caattcctga tcacattcat caatttgctg acgaaaaacc
     2221 tcaagctctg gtggagtatc ttgattcatg tttacccctt ttcaaatacc tctaaatgcc
     2281 ctacatcatg aaatatgaac gataaaaaac caacccccga aacacaggcc gccgcgaaaa
     2341 aaaacaaagc agcagaacaa cacaaaaagc catctgcaaa gacagaaaaa gaagttggcg
     2401 gctttgccga ttccggcctt ctcgaaccca cccgctttgg agattgggaa gttaaaggac
     2461 gttgcagcga cttttaaaaa taaaacaaaa tgaacaaatt agccatttac atccaattaa
     2521 tacccctact cctctgtgca tcgagtatgg ctttttatag cccccaggca caggcaacac
     2581 aagcaacgca aagtgcacag acaatgccaa ttgttgtaga gcttttcacc agtaaattct
     2641 gcccggcctg cccaaatgcc gatcacaatt ttaaccaact catcgcgaaa gatcccaata
     2701 ttatcggcct gtcatgtcat gtaagctact ttaaccgcaa cgggcgtggc gacaaactcg
     2761 cccgcccctt ttgcgacgcc cgccaaaatg tatataaact cgcccttaat accggcggca
     2821 tctatacgcc catgaccatc atcaatggca gcgccgtaac aacaggcatc aaacaaaacg
     2881 aactcaaccc cttaatccaa aagacaaaaa cggcaaaaaa ccgaccggtt ggttttttgc
     2941 gcaacggcga atatcttaac atcagcctcc cacaaataaa tctgccccac ggtgcggaca
     3001 tatggctttt tgaaattgaa aatcgccctg atgaagaaaa ctacacacac taccgcaaca
     3061 cagtgaaaaa catcaccaag ctgctacgct gggacgggaa gaaactaaac atggcttttc
     3121 cggcagagca aaaggccggc attagctatg cccttatcat tcagacatat aaaggcggca
     3181 tcatcgccgc cgcgcaaaca ggatcctaaa agtggcaaaa aaagaaaaac ataaaaaccg
     3241 gcaaacactt gaagaaattc tacaatctat ctccggtatt atgtttccgg cagaaccaga
     3301 aaaacatatc gacataaatt gccatggtta ttgtggagac acgccgctac atgtaatgct
     3361 ctggaataac aataaccacg gtgcggagtt actgatcgaa gctggcgcaa acgtcaacac
     3421 aaaaggcgaa atggacgaaa caccgctaca cgttgcatta cggcagaaaa acaccaaact
     3481 cattaaactg cttctgcaaa aaggggcaaa agacaatata aaatcagaat ttgacgaaac
     3541 acctcgtgaa atggcaaggt ttatggacaa ggatatccag aaactgtttc agttcaaaaa
     3601 cctctaaaaa ccagcattta aagttctata tcaacaatta caggtgcatg atcagaaggc
     3661 ttttcccaat cccgtgcttt cgaaaacacc tgatgcgcct ccagcgattc acgaagcggc
     3721 ggcgttaccc agacatgatc cagccgccgc ccgcgattag attttttcca atcccggtta
     3781 cgatagctcc accacgtata acacttctca tccatcgcca caaagcgccg cgcaacatcc
     3841 acccaatcta gcgaaccaag cataccggcc agccggtctg tttccggagg cgtatgtgaa
     3901 acaacattta aaagctgctt atgcgaccat acatcatgct cataaggcgc aatgttaaaa
     3961 tcaccgaccg ccaccagctt ttgatccgcg ctatagcggt ccttaaacca gtcggtcatt
     4021 tcatcaacaa aatccagctt atgcgcaaac ttatcattca cattcgggtc cggctcatcc
     4081 ccacccgccg gaatatacag attatgcagc tcgaatcccc cggaaggcgt ggagatactc
     4141 gccgcaatat gccgacaatc ctcgcgccca acccggtggt gtacatcgcg cgcatccatc
     4201 ggcaaacgcg acaatatcgc cacgccatta taacccttca tcccgtgaaa atgctgatgc
     4261 tcatagccca tcgcccgcaa atcaccagtc ggaaaatact catccgggca tttggtttct
     4321 tgcaagcata aaatatccgg cgcagcttct tcagtaagtc ttttcactag cggcaagcgc
     4381 aagcgtacag agtttatatt ccaggtcacc agccgcatgc acgctcctct aatcccgatc
     4441 caccgccaaa ggtcccggtg cctgacgggt tggcattaac tcaatccggt ttatattgac
     4501 atggctgggc tgacttgctg cccaaaaaac gctttcggct acatcatcgg ctgtaagcgg
     4561 agttgtgttt gcataaaccg catccgcagc tttttgatcg cctttgtagc gaaccaacga
     4621 aaactgcgtt ttcaccatgc ccggttcaat attggttacg cggatatttt tgctgtacgt
     4681 atcagcccgc aaagccaaag aaaactgctt cacaaacgcc ttcaccgcac aataaacatg
     4741 cccacctgga tacgggtaat tccccgctgt cgaaccgata ttaatgatat gcccacgccg
     4801 ccgcacgctc atcccctgca aaacaggatg tgtcaccgcc accagtgcac tgttattgat
     4861 ctcgatcatt ttcagccaat catccagcgt actttcatca gccggagatt gccccagggc
     4921 cccgcccgca ttattgatca gcaaatcgat tgcaccaaac gcttccggga tagagtctat
     4981 cgcctcggct atcgcagctt gatcggtcac atcaaagcaa agtgtatgcg cacgtacata
     5041 cggaaattct tcagccaacg catcaagctt ttcctgcgaa cgtccatgta aaataagcgc
     5101 agagccaacc cctgcaaagc gccgggcgaa cgcccggcca aaatcacccg ttgccccggt
     5161 aatgaacact attttgggaa tgtaacccgt cacacttaaa acggcagctt cgtgcccgca
     5221 ggcaagccca gcccttccat catacccgtc gtttcggttt taatgcgctc gtctttagca
     5281 tcattggcgt tattcattgc cgcaacgatt aaatcttcca atgtctcttt atcatcaaca
     5341 ttgatgacag aagaatcaat tgcgatagcg cgcacttcac cgccgcaaga catcgtcacc
     5401 ttgaccatgc cgccgccagc ctcgccttcg acctcgatat ccttcatctt ttcctgcaac
     5461 tcttcgagct tgaattgcat ctgttgagct tgctgcatca tatctttaaa attcatcatg
     5521 gcttgtcatg tcccttattg ttaaccattt cgattgattg tatttccgca tctggaaaca
     5581 cgcttaaaat ttcctgcatt atcggcagct tgataatctc tgctcgctcg gcctccaagc
     5641 gcgctccggc cacctgcgcc aacgtcggct ctccgggcgc acctgagaca ctgacaatcc
     5701 agcgcgcttc tgatatccgg ctcagcgcct gtcccagatc ctgcgccatt ttcggcgatg
     5761 cctcggcttc aggacgaatt tctatcaatc ccttctccag cttcaccaaa tgcgcatatt
     5821 gatacacctg tgaagccagc aacacctcac gatgttcttc cagcacccat acaatatctt
     5881 caagattgcg aatttccaag ggcgatggtg gctcaacctt cggcacaacc gccagagccg
     5941 cacgctcacc gccagatcct ttcccggaag gggggccagc aaaagaccga ccggtcggta
     6001 tgcctgtgcg tgctccagca ttcaaggatt gagacggaga agacgcgttc gaaaccggcg
     6061 cacctacagg agcagatgca gactgtgaaa tgccgccagc atttgatgtc acaggcccgc
     6121 tctcttgccc ttgcaaggtt ttgatcaacg ctgccggatc tggcaaatcc gccgcataag
     6181 ccaagcgtat caacacaatc tctgcggcgc tttgcgggtt gggcgctgcc tgcacttctg
     6241 ccaaaccttt caacaatatc tgccacgttt tgcccaatgt cggcattgaa agcttcccag
     6301 ccaactctgc cgcgcgttta acctcatcgg ctgccatcgc atggctcatt tctttggctt
     6361 caggcacggc gcgcaacttc gtcagcgtat gcgtcaaatc cagtaagtct tgtataagtg
     6421 ctaccgggtc tgctccggcg cgatacaaat catccatgat ttccaacgcc tccggcatat
     6481 cgccgctcaa agctcgctcc agcagatcaa gtgaccgcgc ccggtcagct agccctaaca
     6541 tatcttccac ggcctgcgat gtgatagatt ccccattcaa tgccattgcc tgatcaagaa
     6601 tcgacagtcc gtcgcgcacc gacccatccg ctgcacgtgc gatcatcgca atagcttcag
     6661 gttcggcctt aacgctttct ttttcgcaaa tcgaagtaaa atgcgcggac aatgtcggca
     6721 catccacccg gcgcaagtca aaacgctgac accgcgagag cacggtaatc ggcactttac
     6781 gaatctcagt cgtcgcgaaa ataaacttca catgttccgg cggctcttcc agcgttttaa
     6841 gcaaagcgtt aaacgcgttt ttcgaaagca tatgcacttc atctatcaca tagaccttat
     6901 aacgtgcctc gctcggcgca tagcgcaccc cgtccagaat ttcacgaata tcatcaacgc
     6961 ccgtgcggct ggccgcatcc atctcaatca cgtccggatg ccggtcctcg gcaatcgcct
     7021 ggcaaatttt acaatcctca gtcgcccccg ttgtcggccc ggccttacca tcagccccga
     7081 cataattaag cgccttggca ataatccgcg ccgtcgtagt cttcccgacg ccccgtacac
     7141 cggtcaacat aaacgcatgc gcaatccgcc ccgattcgat cgcattggtg agcgtacgca
     7201 ccagcgcatc ctgaccaatc aattcatcaa aactttgcgg acgatatttc cgcgccaaaa
     7261 cgcggtatgg aacttgagcc tcagacatac cttatatgta gggcgattcc cttaccagaa
     7321 caacaccaaa cagcttttca tttagacagg aaaaaacatt aagtggaaga cggacacgac
     7381 ccgaaaaaat cacttacggc tgctggctct cgcccctgac cgggttgggc aatatctcgc
     7441 ccgcgccgcc ttccggcatc tatatagaaa acctgcatcc aaaacgcaag gggtgatgaa
     7501 ttttcttcgg ccagcatatt tcacttcgca tcactttatg ctttaacagc atccatgaaa
     7561 aatctggtct cactcctcac ccgcctcgcc gatgccgata tggtgttctg gctgctgccg
     7621 ccgctcatgg cgctgctggt ctccggaacg ctggcccagc gctggatggg tctctggccc
     7681 gcgatggaaa tgttcttttc cagcttcatc atttgggccg gccctgtccc actgcccggc
     7741 gcttatacat tgctcggcat tctcagcata aacctgacac tcaaattcct gcttaaaagc
     7801 cgctggcaat gggaaaaatc cggcatcatc ctctcacatc tgggcgccat cattctgctg
     7861 ctcggtggcc tgctcaccgc catgactgcc cgtgaatatt acatgctgat ccccgaaggt
     7921 caggaaagcc cctttatcta tgagtatcac cgccgcgctt tgaatatttt cgaaaatgac
     7981 cgccagatcg cccgtctacc ctttgaaaac attgaaaact gggacctgtc agccctgcca
     8041 ttccagatac gcatacaaaa ttcatgtcaa aactgcgaca tccaaaaacg ccaggaacaa
     8101 ccgggttacg atccggagca gccctggcag ggtatggcgc gttttatggt cttcgaccct
     8161 aaaccaaatg aaaaagaccc ggaaagcgat ctctccggcc tgcagttcga agtaacagga
     8221 acggatcagg acggcatcta cgtcgccttc gatgccatgc ccaaaccaat tgaaattaca
     8281 aaaggcgaag ccatctatcg cctcatgttc ggtaaacgcc aaagcatcct gcccttcact
     8341 attgccctta ctgatttcgt caaaaatgaa tatggcggca ccgctatggc gcgcagttat
     8401 cactccgaca tcatcatcaa agacggctcc ctcgaatggc ctgcacgcat tgaaatgaac
     8461 aaaccgctgc gctatcgcgg ctacacattt ttccaatcat catttgaaca aggccccgat
     8521 ttcgaagcca ccatcctcgc ggtcgttgaa aataaaggcc gcctgttccc ctatatcggc
     8581 agcatagtct taggccttgg cctgatcctg catatggggc ttatactgat ccgaaaaaac
     8641 acaaggagca aagcatgaga atttttttga tccttgcctc gatcatcacc gcattattac
     8701 tcccccaaaa tgcaggggct gaggcgcatt ttgaaatgca gtatttcaaa acattgccaa
     8761 tcctgcacga aggcagagaa aaaccgttat ccagcttcgc cgacatcatg ctgcgccaat
     8821 tttcaggaca ggaaaaactg caaaatatgg atgcaagtca gtggctcacc cttacacttt
     8881 ttgacccgca aagcgccgcc gagttgcccg tctttaccgt gtccgatgaa accctgatca
     8941 ccaaattaaa actggataaa acccagaatc tctacagcta tgcgcagatc cagcccgctc
     9001 tcaaagccat gcgtgatgaa gcactgccgc ttttttcaaa agaagaaacc gccttgaccg
     9061 ggcaagaaaa aacgctgctg cgcctgtatg aaaatacagc gctttttacc gcgcttttgc
     9121 gcagcttcac ggcactgctc ccgcttgatt tatcactccc cccgcctatc aagaccaaat
     9181 cgatggagca ctgaatttta ccgaactgct aaaagtcgaa aaacaactgg aacaagacct
     9241 caccggaatc attacacgaa aaggccgcga tccatcgaaa tacacacccc gggaattgac
     9301 aatcgccaaa gcctctttcc acctgcaaac tctgcgtgca ggcgctcagg ataatgagct
     9361 actacgcatc atcccggtgc aatgggaaga cagcaaagac caatgggcca cgccatggac
     9421 aatcatgctg caaggtcagg gtgggcctgg ggccgcattt ttactctccc aatggacgga
     9481 tttagcgggc gcctaccgcc aaaatgatgc ccggcgctgg aaaacaataa gcgaagacat
     9541 ccttgaagaa accctgctcc aaagcccgca aagcctgaac atcaaacgcc tgaaaatcga
     9601 acaactctac cgcactgttc acccttacac gctgataata acgctttacg gactcagcat
     9661 ttttgctgca acatttttac tgttcaaaca accaaccgcg cgccttctga gattagcgcc
     9721 aacccttctt gctctcacag gcatcgtact ccatatagtt accctcacag cacgaatata
     9781 tattctgcag cgcccgcctg tcggcacact ctacgaatcc attttatttg taaccctgat
     9841 ttgcgcagcg cttggtattt tattgcaacg cgcccgcacc tcattcatcc cgttaataac
     9901 cggaacagga accgccgccg cgctcctgat ctgcgcaccc gtctttaaac cggatggtga
     9961 cagtctcgaa gttcttgccg cagtactcaa caccaacttc tggttaaccg ttcatgtgct
    10021 gtgcattacc gccggctatg gagtctgcat tctcgccgcc atgctcgccc attccgggct
    10081 gtatatcagc ggcttcaaac acaacaaaga actatcgaaa aaactacggc aaaatgtcca
    10141 ccgcacctcc atcgccgctc tgctgctcac ggccataggc accgctctgg gaggcatttg
    10201 ggccgaccag tcctggggcc gtttctgggg ttgggaccca aaagaaaacg gcgcactgct
    10261 catcgttctg tggcttgtct gggtacagca tggccgcatc agcaagcatc tctcttccac
    10321 aggcttcatg gccatgagcg ccgcgctgaa catcatcatc gccatctcat ggtttggcgt
    10381 caatttactc aacgttggat tgcattccta cgggttcacc agcggactcg caggcagctt
    10441 aatagccttt tgcatcgctg aaattgcact catcacattt ctatatatcg cagcaaaaaa
    10501 acaggaatgc gcagcatgaa aataacccct ctgatcaatt tcgccgttct tgtcttcatc
    10561 gggtgcgccg cctacctagc tacgctttac cttgaccgtc aagcgcccga tatcaaaacc
    10621 gcccccgaat cggttttgaa acaaaccgaa aacaaccaat ccgtgcccaa tttttccttc
    10681 accggcacag acggcaaaac cctctcaata aaagacttcc agggtaaagt tatcattttg
    10741 aacttctggg cttcatggtg cacgccttgc ataaaggaac tccccctttt tctaaaagct
    10801 gcaaacgaaa accctaaaga cctgattttc atcggcctat cctcagatct ggaaaaagaa
    10861 aaaatgatac gtttcctgaa aaaacaaaaa ttcgacatct ctcaaaaaaa tatttttttc
    10921 gctctggatt ccgaaaacat tacccaaagc cttttccaga cttttcgcct tccggaaaca
    10981 atcctgattg ataaaaacca gatcatgcgc acaaaaatca tcggcgcaaa ctggacttat
    11041 gaagagctaa tatcaaaaat aaaggccgcc caaaccttac aacaataaat tgtgaaccat
    11101 atcaccgtat tctcacatta tttttataca cttctcaata aacgtgctac catacggctc
    11161 tgcgcgtatc gattttaagc atacttaatg gggaaaatat ctatgcaaaa tgctgccacc
    11221 aatcttgtac cgttcatgag ttcgaagccg gaagccgtaa ctatcctcaa tatgttgcca
    11281 gtcaatgtca tgacatgcaa cccaaacacg ttcgtcatcg actacgcgaa taaacgaagc
    11341 atagaaacgt taaactccct tcaacatctg ctacctgcag gtatttccgg tgacaacatc
    11401 gtcggtcaat gcatcgatat tttccacaaa acgccgcagc gtcagcgcaa aatgctggcc
    11461 aatccatcaa atttaccgca ccaagcaatc atccgtttgg ggccggaaat gctggacctc
    11521 catgtcgatg ccatcatgtc aggcaacaaa ataaaaaaac tgcttttaag ctggagcgtc
    11581 tgcacagagc gtgaacgtct gaagatcatg atcgacaata tgccgatcaa tatcatgatg
    11641 tgcgatccgc aatccctgaa actaaccttc ctgaacaaga ccagtattga aacattgcgc
    11701 tctatcgagt atgccctgcc agtaaaagcg gataacatga tgggggcctg tattgacgta
    11761 ttccacaaaa acccgcaaat gcagcgccaa ctcttgggcg atcccaataa tttgcctcac
    11821 aaagccaaaa tccgtgtggg cgatgacgtt ctagatctaa atgtagccgc tattctggat
    11881 gattccggtc attatattgg cccgatggta agctgggcgg tgataacggc gcaagaaaac
    11941 ctctccaaat cagttctgga ggtatcagaa agtgtttcag cctcgtcaaa cgatgtgcaa
    12001 acaacggccc aagccctctc tgccgccgct gaagaatcat cggcgcaagc cacatccgta
    12061 gcagccgctt ccgaagaagc ttcaacaaac gttcaaactg tagcagccgc aaccgaagaa
    12121 atgaccgcct caatcaagga aatttcagcg cagattgcaa aatccaacga aacagccggc
    12181 gaagccgtaa aaaaagccga ggaaaccaac agcacagttg aagccctgca cgaagcctcc
    12241 aaccagattg gtgaagtcgt caatctcatc aacgacattg ctgaacagac aaatcttctg
    12301 gccctaaacg caaccattga agccgcacgc gctggtgagg caggaaaagg ctttgccgtt
    12361 gtcgcctcgg aagtgaaatc actcgctgcc caaacagcca aggcaacgga agaaattcga
    12421 acgcaaatcg ataagatgca atcaaccacg tccgatgccg taactgcaat cggttcgatt
    12481 cgcacgacaa tttcagccat cagcgatgca acaagctcga ttgccgccgc gatcgaagag
    12541 caaacggcta ccacatcgga aatttctcgc aatgtgcaag aagcctcacg cgccacagcg
    12601 gaagtaactt cgaacatatc cggcgttcaa caagccgcct ctgaaacagg atctgcctca
    12661 acgcagcttc tcgaactggc aacccaactc tccgaacgtt caaatgaaat gaacaaacag
    12721 gtcaacgatt ttatgaacgc cggccagaaa agcaaaaaat aaatgaaaac tcaaacctgt
    12781 ataataacct caacgccccc ttccaggggc gtttttcttg gcaagtcccg atcagtacat
    12841 ccacacagca cccgataaaa aaacaaataa tgattcttgt tttcgtgacc ctagtgtatc
    12901 atacgtactt ccgtaaatgt taaacggcaa cataaacgac aaagcaacgc ccatgtctaa
    12961 atctctgatc aaagaagctc tgaaagctct tcccgggaaa ccttccgcaa acaaaacaga
    13021 gcttctcgaa aaactcgcgc aaagcatgac gcaagaagat caggatcatt tcaaccatac
    13081 attactggcc aagatcgtag aaatacacga aaaattggcc gaaaaaagag aaaatggaga
    13141 cccgcaactc aaaatataca ccgttacaga ttcacacctc ggaccgcgca aaaccattat
    13201 cgacgttgtc agcgatgaca aagcattcct cgttgactcg gtcgtcgccg aagtcaacaa
    13261 aaactcattc ctgattgacc tgcttcttca tccaatcatc tatgccaaat atagcgaaga
    13321 cggcaaattg ttaaaaacct ctccgacaga aaaagaaggc tttatccggc aatcccacat
    13381 ccatatccaa attaaggaca cactaccgga aagtgctgta aaagcgttag aaatcgggct
    13441 gtatgaggct gtccaagacg tgcacatcgc caacaaagac tggcgcgaga tgctcgaaaa
    13501 gctcaaagac gcgcggcaag ctctggaaaa cgcaaaaacg cgccgcccat taaaagaagt
    13561 ccagcaatat tgcgcctttt tagactacct gcataacaac aactttaccc tgctgggcta
    13621 ccgggaatat aaattcgttg aaaaaaacgg cgaagtgaca agccgcaccg tgcccagttc
    13681 cggtctaggg ctgctacaca aagaagttcg accggcctat atcaacgatc acgatgaagg
    13741 cctgccgcga aacctgcagg aaaaacggcg taacctgcca ccgctctcta tatcaaaaac
    13801 aaaccgcctg tccactgttc accgccgggt tccgatggac gccattgcca tcaaaactta
    13861 tgacgaagac ggcacgatta ccggagaaaa actctttatc ggcctgttta cctccgtaac
    13921 ctattcccgc agtgtcggcg acgtaccata tatccgtgaa aaagtcgaag aagtcatgga
    13981 aatggccggc tattctatag gttcacacga ccgcaaagct ttgcggcaca ttttggaaaa
    14041 atatccccgc gacgaactct ttcagatcag tacaaaagaa ctgttaaaga ccaccaccag
    14101 catcctgaag cttcaagaac gccagcgcat cgcactgttt atgcgcaaag atccgttccg
    14161 ccgctacgtt tcttgcctgg tctatattcc ccgcgaccgc ttcgccacat ccttacgcaa
    14221 aaccatgtgt tctattttag aaaaacagct caacggaaca tgcgacacat tttatacatc
    14281 actcgatgat tccgttttcg cacgcgtcat gttcatcatt aacatccgac aaactgatga
    14341 ccccatgctg gatgtcaaac gcatcgaaaa aatgctccag gaagctggtc aaacctggca
    14401 agagcttctt gcacaggctc tgagcgaagt atacgccaat gaaaataaca tcacagctac
    14461 gtgccaaaaa tatggcgagg cttttcccgt tgcttacaca tcacgttacc gcccaaaaca
    14521 ggcactcttc gacatcgata aaatcgaaca agtcctggac acaaaaaatc tgcaactgga
    14581 cctctatcgt cctgacgaca tagatcctaa acaactacga ttaaaagtat acaacccgca
    14641 acgtccctta acactatctg acgtcatgcc cattctcgaa aatctgggtc tgcgcgcaat
    14701 ttccgaactt cctttcgaag tgcgcccata taaggaaaaa caatccatct ggattcatga
    14761 cttcctgctc gaaacgcccg agatggatga gatcgtagac gtagcatctg taaaacagaa
    14821 ttttgaacgc ggattttcaa aaatctggag tagcgagatg gaaaatgacg gcctaaaccg
    14881 tttgattctt gccgctaaca tgaactggca cgaaatcacc atcctgcgta catatatacg
    14941 ttacatgaaa caaatccgct atccgctcag ccgcccttac acagaaaaag cattaaccgt
    15001 ccatgccaat atcagccgca tgctggttac tttattcaaa agcctgcacg atccaaagca
    15061 tcaaaaaaca tccgccaaaa aagccaggga atgcatggca aacatagaaa acgcccttga
    15121 gaaagttgaa tctctggacc acgaccgcat tttgcgtaca atcagcaaac tcatcgaggc
    15181 aaccttgcgc acaaactatt accagcgcct cgataatggc cgcgcaaaat cctatctctc
    15241 tcttaagatt gagagcgcca aagtaccgga aatgccagcc ccacgtccct atcgtgaaat
    15301 ttttgtttat tcgcctcgcg ttgaagggat tcatctgcgc ggtgataaaa tcgcccgcgg
    15361 tggcctgcgt tggtcagacc gtcatgaaga tttccgcacc gaagtcttgg gactcatgaa
    15421 agcgcaaatg gttaaaaacg ccgtcattgt gccaatgggt tccaagggcg gcttcgttgt
    15481 caaacaacca acccacagcc gcgaagaatt catgaaagaa ggcatagagt gctacaaaac
    15541 cttcattcgt ggcctgctcg accttaccga caaccgcaaa ggcaacctta taattccgcc
    15601 gaaagatgtc ctgcgccgtg acgatgatga tccatatctg gtcgtcgccg ccgataaagg
    15661 caccgccagc ttttccgaca tcgcaaacgg catcgcccgc gaatacggct tttggctcga
    15721 cgacgctttc gcctccggcg gctctgccgg atacgaccac aagaaaatgg gcattaccgc
    15781 ccgtggtgcc tgggaatcgg ttaaactgca tttccgccaa cttaatcacg acacccaaac
    15841 ccaggatttc gatgttgtcg gcgtcggtga catggggggc gatgtctttg gtaacggcat
    15901 gctgctatcc aaacatatcc gcctgatcgg cgcgtttaac cacctgcaca ttttctgcga
    15961 ccccaaccct gatccggcta caagctggga cgaacgtaaa cgtttgtttg atggcgtaaa
    16021 aggatggggt gattacgaca ccaaaaaact ctccgccggc ggccgtatct tcgaacgttc
    16081 agaaaaaacg ctcacgctca cacctgaaat tcaaaaacgt ttcgatattt caaaagaaaa
    16141 agttaccccg ggtgaattaa tgcgcgccat gcttaaagcc cgcaccgacc tgctctggtt
    16201 cggaggcatc ggcacctata tcaaatcaag taaagaaagc cacgccgacg tgggcgataa
    16261 agcaagtgat gccctgcgca ttaatgcgcc tgatttacgc gctaaagtca ttggcgaagg
    16321 cgccaacctc ggcgttacac aactgggccg tatcgagttt tgcgaaaacg gcggcaaaat
    16381 gaacaccgac tttatagaca attccggcgg tgtaaactcc tctgacctcg aagtgaacat
    16441 caaaatcctg atgaccgatg ttatgggcgg aaaaacacac aacatggaca taaaggcccg
    16501 taataaactg ctggaaaaaa tgaccgatga agtcgccgga ctggtcttgc gcaataacta
    16561 ccagcaagca caagcaatca gtttggcgga actgcaagca cgtgaaaatc ttcaaattca
    16621 ggatgaattc atgaaagacc tggaacgcga aaagggcctt aaccgcgccc ttgaaggtct
    16681 gcccgaagaa gaaaaaatcg aacagcgcct acgcgccggc aaaggcctga cccgccccga
    16741 actcgcagta ctcgtatcct acgccaaaat atccttcaca caggacctga tggcctccga
    16801 cattcctgaa agtgtggata tggaagactg gattatcaat tacttccctg gcgatctgcg
    16861 aaaaaaatac atcaaggaaa tcaagcgcca ccgtctggcc cgtgagattg tcgccacctc
    16921 catggccaac tcgctcgtca accgtatggg cccaaccttc ctcaaagaaa aaatgaacaa
    16981 aaccggctcc agcgctgccg aaatcgccaa agcctatatc atcgtacgcg atgcatataa
    17041 tctgcgcgcg ctctgggatc aggtcgaagc cctcgataat aaagtaccgg cagaagtcca
    17101 gctcaaagcc atgcgggaaa tggcgcttct ctcagaacac tccatcagtt ggttccttac
    17161 ccgattaggg catgatttgg atataggtcg tgagattaaa gattttggcg aaggcataaa
    17221 aaatctcagc agcaatctca gtaaactcct gaccaatgat cttaaagcca gcgtcaacat
    17281 gcgcattgcc gcaggacagc gcgacggtct acccaaagcc ctggccgaac aaattgccct
    17341 catgcctgta ctatcctcgg catgtgacat cattcgcatt gctctcgaac aaaaaaccga
    17401 tctattgaaa acagcccgaa cttatttcga agtcggcgaa gaatttcaca tggactggct
    17461 acgccagcaa gcacgttacc tcccttccga cgatcattgg caagccgaag ccgtccgtgg
    17521 cctcgtcgac cagctctttg gcgcgcaagc cgccttgact gtacgtatct taatcgacac
    17581 caaggacaaa acaaacaaag gcaaaacacc cgcagaaaca tggatggatg accacgcaca
    17641 taaagtcatg caactcacac cgctcttcgc agacttgcgc cgcgccggaa cgattgaatt
    17701 gccgatgctg atcatcgcag agcagcgcct gcgcaatctg gcaggttgtt aaaatccgcc
    17761 ggcaaaaata acggcaagga ccaacaaccc taaatcgcgg ttggacttaa acacggccaa
    17821 agagcacgca ggatcatcca tatcccagcg cccgatctgc cagaacaaat gcataccaat
    17881 cagcgccaac cccggaagtg caaacgcatt cagcgaagac agcgcaaacc cccatcccag
    17941 aaacaccagc atcaccgcat aaaacccgct cacccatttc ttgctgttct caccaaattt
    18001 tagcgcagtc gatttcaccc cgatgagagc atcatcctcc ttatcctggt gcgcataaat
    18061 cgtatcatag ccgagcgtcc acaaacacgc cgccacatac aataaaagcg cctgcgcgct
    18121 taaaccatcc atcaccgccg cccagcccat aagcgcaccg aaattaaacg tcaacccgag
    18181 aaccgcctgc ggccagaacg tcactcgctt catcagcgga taaataacga tcagaggaac
    18241 agccagtaaa cccagcaaaa tggttatgcc attcatcaaa ataagaatac aaaaaccggc
    18301 catcaataac ccgaacagaa aaataagagc ttgctgcaca ctcacttcgc cactggccaa
    18361 gggacgtacg cgcgtacgat cgacctgtgc atccagcttc cgatcccaaa tatcattaac
    18421 cacacatccg gctgcgcgca tcacggaagc gcccagagca aaaaggaaaa tcaaccacaa
    18481 aactttaaag ctaaaaccgc tcaaatgcga agcggccaac gttattcccc acaaggccgg
    18541 cagcaataaa agccaaatcc cgatcggacg gtccagccgc gccagataga catacggcct
    18601 gaaacgttca ggcaatttct caatccagtt ccataatcga atatcggtaa acatgaccac
    18661 atgatgcctg cacaccgccg cacgaacaat cctaaagtaa taaaatatat atgaagaact
    18721 gcaagcttat gatttattac aaagattatg aaagacacac acaaaacccc gcgtctctac
    18781 attgatcagc cgctaaatgc tggtaaagat gtgatacttg aaacgccgca agcccattac
    18841 ctgaagtcgg tcttgcgctt tgaaaaaggc acacctgtac gtgtttttaa cggccgggat
    18901 ggggaatggc tcgccacaat ctctgcgctg gagaaaaaat ccgctgtgct aacgctcaca
    18961 aaccaactgg ccaggcaacc cgtacaacct caaccgttac atctgctctt cgctcccatc
    19021 aaaaaacacc gcatggattt cctgatcgaa aaagccgttg aactgggtgt aacagacctc
    19081 acacctgtca tcaccgccca cacgcaaaca cgcaaaatca acgaagagcg ccttcacgcc
    19141 caaatcatcg aagccgccga gcaatgcgaa cgtatgagcc tgccaaccct tcataaagtt
    19201 gaaacactgc ccggtgcact gcaaaaatgg aaccaacaaa tacctgtata ttgggcggcc
    19261 gagcgtttaa aaaatacgcc ccatatcaag gaaataataa gccctgaggc tttccttatc
    19321 ggccccgaag gtggtttcag cgaacaagaa agcgactttc tgcaaacaca ggaaatcgta
    19381 aggccaatct ctttgggaaa aaatattctg cgcgccgaaa cggccgctct tttttgcctg
    19441 tcccatgcga atccgtgaaa gcattatcta tatgtttaat tgggatcaat tcagcatatt
    19501 gacctgactt gttatagcct atacgctcat ccattagctt gggtgttttt gaaaaacatg
    19561 cgggcttttg gaaacccgtt ttacaactta aagcagcaga ttttaagaca atgatcaaaa
    19621 gattcctgac ctttgccttg gccgtatttg tctcatttcc aggtgccccc gcttttgccg
    19681 gacaaccaat gccttggcag attggcctgc aagaacccgc atcaccttcg gcgctgcgaa
    19741 tttttgaatt ccacgacatg ctgctctata tcattttctc catcgcaatt tttgtcctgt
    19801 tgctgctggt ctgggtcatt atccgctata atgcaaaagc caatccccag cccgcacaat
    19861 tctctcacaa tgtcttaatt gaagtcctgt ggactgtgat tcccatcgtg atcctcatca
    19921 tcatagctat tccatctttc aaggtcctgt attacaacga ccgcgttgaa aacccggaca
    19981 tgactctgaa agttacagga tatcaatggt attggacgta cgaatatcct gatcatgacg
    20041 gcttgaactt ttcatcctac atgatcgccg acaaagacat agacgaaagc aaagaccaga
    20101 aacgcttgct ctccaccgat aatgtcgtgg tcctgcccat cgacaccaat atccagattt
    20161 tgatcacagc cggtgacgta attcactctt gggctgtgcc agctttaggc atcaaactcg
    20221 atggtatacc gggccgcctc aacgaaactt gggtccgcat cgaaaaagaa ggaacatact
    20281 atggccaatg ttcagagctt tgcggcaaag accattctta tatgcctatt gaaatccatg
    20341 ctgtcagcaa ggacgaattt aaaaactggc tgataaccgc caaaaaagaa tttgcatccc
    20401 ttgatgcaag cgacgattcg ctaatccgca tggccggatt tcagaaataa aaacaacaga
    20461 taaagaacaa ataaaccaag gaagacgata acaatggctg atcataaacc gggatttttt
    20521 gcgcgctggt tcatgtctac gaaccacaag gacattggaa cgctctacat cattttctca
    20581 atctttgccg gaattgttgg cggcctgttt tccatgatca tgcgtatgga acttcaggac
    20641 cccggcgtcc agttcatggt cgatagcgcc ggaaaccccg atggccatat gtggaatgtt
    20701 ttcattaccg cccacggcct catcatggtc ttctttgtcg ttatgccggg cctgataggc
    20761 ggtttcggca actggtttgt cccgctgatg atcggtgcgc cggatatggc cttcccacgc
    20821 ctgaacaata tttccttctg gcttatcatt ccggcattcc tccttctgct ttcttctgca
    20881 gtgatcggcg gtggagcagg aacgggctgg accgtctacc cgccgctttc gagccttctg
    20941 ggccacccga atctcgctgt tgatatggca attttctcgc ttcacctcgc cggcgccagc
    21001 tccattctcg gcgcagccaa cttcatcacc accatcttca acatgcgcgc gccgggcatg
    21061 acgctacata aaatgccact cttttgctgg ggtatgctgg tgaccgcctt tttgctggtc
    21121 ctcgccgtac ctgtgcttgg cggtgcgatt accatgctgc tgaccgaccg caatttcggc
    21181 acctccttct ttaatcccga aggcggtggc gaccctcttc tctaccagca cctcttctgg
    21241 ttcttcggcc accctgaagt ttacatcatg atcctgccgg ctttcggcat catcagcc
//
LOCUS       DAOSWR010000016        20325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1655_length_20325_cov_5.356981, whole genome shotgun sequence.
ACCESSION   DAOSWR010000016 DAOSWR010000000
VERSION     DAOSWR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_20325_cov_5.356981"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..356)
                     /locus_tag="PK513_02280"
     CDS             complement(177..356)
                     /locus_tag="PK513_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81312.1"
                     /translation="MTAVPAGIAAERAILRQNVALSVIKQSADQAQALVQILDDAARS
                     APISSTRGTNINTSA"
     gene            complement(434..928)
                     /locus_tag="PK513_02285"
     CDS             complement(434..928)
                     /locus_tag="PK513_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81313.1"
                     /translation="MKNTQNVKFITPPNRLKMKVGGGGISEERIQRAQEVINNFEMDF
                     RPEARKLAITLDKATNDAIQSIKSKKVFNKEKMIHPVMQLKANGGMFKYCLLTDVADI
                     CLQFMEAVHEYNTEAIEIIKAHENAIQIIIKNDLKGDGGPEGYTLVQELHKACTRYFK
                     KYKT"
     gene            complement(936..1502)
                     /locus_tag="PK513_02290"
     CDS             complement(936..1502)
                     /locus_tag="PK513_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81314.1"
                     /translation="MPYNLNDVKILLVDDMKPMLTLTKSILTTFGFTHIFTAQNGEEA
                     FNLLCKHDPDFIITDWIMEPMDGLQFTKKVRTDPRSPNPYVPIIMMTGFSSKLRVEGA
                     RDNGITEFLVKPFTAQDLYARVFQIIEKPRKFVDTGEFFGPDRRRKSGKGYEGPRRRD
                     EDPVNTPTQNTPEALNILKKLSEEAQKI"
     gene            complement(1599..2147)
                     /locus_tag="PK513_02295"
     CDS             complement(1599..2147)
                     /locus_tag="PK513_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO81315.1"
                     /translation="MKQSEAFERLTKIAEDRNALIVADIGSQSLWLKEAKDRPENLYL
                     SGPMGMASSVALGVAMACKDRAVIAACGDGALGMNLSSLATISNAAPKNLSVVVMDNS
                     IYEFTKSLPTPTRGINWNHISLACPGFQSNIALKDMKDLDLLSGPHFIHAEITPGKLP
                     PDLGMNAIDVHHRFKAYLNNLG"
     gene            complement(2144..2659)
                     /locus_tag="PK513_02300"
     CDS             complement(2144..2659)
                     /locus_tag="PK513_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOO81316.1"
                     /translation="MTKEIEQYETMIKTILDALDKHDINLATGVPDGWLVPMINGLSE
                     DKRFDYIPAAREEECFGIAAGAAMSGQRALVLMQNSGFLNSVGAFTTLCQKYQTPFVC
                     LIAHRGGINDSNSYDPNKYKSFEALTRAVNIFVYPSSWENLPLNIPKAIARSEGAGEP
                     AFITLSEGLKA"
     gene            2866..3297
                     /locus_tag="PK513_02305"
     CDS             2866..3297
                     /locus_tag="PK513_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOO81317.1"
                     /translation="MSAKQIIMNFTVPPSIDDLNVMAASILESLPEELLEFCDSLAIS
                     LDEFPDETLEQELELEDTYELLALYRSGKEISPGVEKKTANDEDVLVLFRRPILDMWC
                     EIGEDLGGLLRQVMIEELGKYFDFSEDEIEEMSERHYQGML"
     gene            3421..4335
                     /locus_tag="PK513_02310"
     CDS             3421..4335
                     /locus_tag="PK513_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81318.1"
                     /translation="MSLGLAVRSILALFVVLSILWVPIHVRAAAAPIEESPGCDPEFW
                     DVLEARTWLEGQREVEAASRLILKADSVLQYTCFNDRISELASAADWIFSDRVVGAPL
                     FTNHPMWRQRHPPPPGVAGASPFTPIYLGPNGPDPPGGAFTALELDKHLNNLVRGPMN
                     TYLGNNFNHTYGGGLGPVGGVCMSMNTVWNFLRCQDYDKDLFISLKDMPGNDPRTLPY
                     TCPNAGARTAKWNLNLLKAFPPPSLPAALGGMDLKEHYNNRMNNNGGCSGAFPISTGV
                     VVKMGGGNHPDAVCSIPGCSYDGSGGCF"
     gene            complement(4332..6260)
                     /gene="htpG"
                     /locus_tag="PK513_02315"
     CDS             complement(4332..6260)
                     /gene="htpG"
                     /locus_tag="PK513_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOO81319.1"
                     /translation="MNAQPKTQDPSPNTHSFSADVSRLLDIVAHALYTNHDVFLRELI
                     SNSADACDRLRYAAIEKPELTADNPQFRIHIYKDTDARTLTVVDNGIGMSKSELIDHL
                     GTIAKSGTAALMEKMKAGEKSGGDPLKLIGQFGVGFYAAYMVASHVRVISRKAGSKSI
                     WVWESDGASGFNVRKASKDEEARLDGDRGTAIILDIKDEAAEFLINEKIKQTVETYSD
                     HIDFKIFLGSPTDTDTGEGQPINKASAIWTRSKNDVTKEQYDEFYKHITHGFDEPLMT
                     SHWHAEGKIEFSALMFIPTLRPWDLYDPSRKNAVRLYVRRVFISDNVEGLTYPWLRFV
                     RGVIDSEDLPLNISRESLQYNPIITRIRTAVTKHILSDLDKLSRDDSIGFSAFWGQFG
                     PAIKEGLYDAEQHREAIFKICRFYSTHDSKEGGGEKFTSLDEYIERMKEDQKDIYYIS
                     GENLQSLKNSPQIEGFRAKGIEVLFLTDTIDDFWLQNVSDYKGKSFKSVTKGDINLGK
                     NKDEKKDEKSTETHKDLLENLKKLLEDDVSEVRISNRLTDSPVCLVAPEGGVDMHMER
                     VLKIHQKYTGTSKPVLEINAEHPLIQKLETLKAKSEDFKNAGLLLFDQAKIIQGEPVK
                     DPGAFARRMAGFISKGLD"
     gene            complement(6347..6955)
                     /gene="pdxH"
                     /locus_tag="PK513_02320"
     CDS             complement(6347..6955)
                     /gene="pdxH"
                     /locus_tag="PK513_02320"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOO81320.1"
                     /translation="MSNKIIEKYGPNPIALCKDWLAEATKSEINDPEAVNLATADARG
                     RPSNRMVLIKDISDEGFKFHTNGASRKGQELEANPYASLCFYWKSTRKQIRIEGTIRP
                     VSDEETDEYFATRSKERQIGAWASQQSETFDDENHMDNAVAKFVKKFTNLDKIPRPPY
                     WKGYRVNPTSIEFWIANKHRLHTRFIYRKNKDGQWDAHWMCP"
     gene            7084..7560
                     /locus_tag="PK513_02325"
     CDS             7084..7560
                     /locus_tag="PK513_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HOO81321.1"
                     /translation="MKNLFLIPLVLVAFVASGCENIQNSGTKEKIGGVSGAVVGGLLG
                     SKVGGGSGQLWATGAGALLGAFIGSEIGASLDKADRVMLGQANQRAQTAPIGESVSWN
                     NPESGNYGTVTPVRDGQSSYGRYCREYEQMIYVGGRQETAVGTACKNDDGTWEVVS"
     gene            7910..9220
                     /locus_tag="PK513_02330"
     CDS             7910..9220
                     /locus_tag="PK513_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO81322.1"
                     /translation="MDNKFLQHKILKSENGFTLIDVAVALLVIGILAAPLVQQYRIWL
                     LTSKQQLTAGRAQNARDAIEQYYFVNNRYPCPADPRLGPSDADFGVELRDFGGTGQCD
                     TSATALGPFTVTSALDADGVGGNDPVYMGMLPFKSLSLSPHEALDSWNNKLTYAVSGL
                     LTNEATFAGIFGGITIREDFVMRHPTVLDATADPAADNVCTDTSLLVPGAVPPAQVYG
                     PVNNVHYVIFSHGKNGNGAYYNANNAAPARACDMAAADAENCNNITNNEATFLYDTCQ
                     SSSASDANNYDDIFVANNITLSSAPFANWNPAVDQQNAGAPTGFYGINNALPEAELDV
                     IGNVRASLDPLDSQELGRLYSHQYCDGNGGNCMKPEAIAGEEPDMRCGFSGTAMRGVA
                     HNQVRCNIVVQTLSSATCPGAPGPQQYVTGFSASGQPCCNNVCP"
     gene            9253..11526
                     /locus_tag="PK513_02335"
     CDS             9253..11526
                     /locus_tag="PK513_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO81323.1"
                     /translation="MNVQTKTTRKPSFPARYSEGGFTIVEMAIVTMIIGLVFAAAFQL
                     LEQRQRFVRAENTKIAVSNANVALAAFVNNFGRYPCPASLNATRDNNGPLYGVESDCT
                     DTGAVAAGSNVDAALGYAVVTGARNIAYFDRSTVPPTRVPAAGIAPVRVRIGALPFKN
                     LNLQEGQAYDGYQNRLLYAVAEPLAVGKTFSAGGGVIDVQNELDASVLQPQASAHFII
                     LSAGENGAGAYGYEGRRAACLGTSEESQNCDFDVDAIFRVAETGTDNNGAAATYDDLV
                     VYALDGDIPMWELEGANDDAILKTVGDIGIGSVVAANNPGEDLQVDGTLIVRDNPGTS
                     ELEGKVESEEICDFTSGSTTCFKSEVIAGDLSHDQGLICGPGLFMTGIQNGAPVCTDE
                     VIVTCPAGKFVTGVDSDGGLICDATPPPSCPSESKNLCSGSGLLPSAPHNTIETITVS
                     TAPTNPGGTRSAKYRCNSGVWEYQSSWGDPCNCTPVIDPVVVNSKASCHDGNNCNGSF
                     TGHVVVTSTTACPSGLSTQVTDRSGCGCLTGTKITPVGCPSGFNKGRKIRSSTYVCNV
                     SGDLTRGACTSPTPAADDPDDPCRCEPVPPFLWAYAPCPLGLKALNDKGIPQNAEFEC
                     SGGSDAPGAWVIYPNKNDPAVIAQYCSCSVPPPSNVTRSDCPPGFSGERTYTITKTCD
                     ILHPLDPPTVTEEIYKDDCLPDAVGMGKWEVVGGAAGAVSPVVHVGGTCILGTDSPPA
                     RLCKKVHGHHVKACLCQ"
     gene            11542..12804
                     /locus_tag="PK513_02340"
     CDS             11542..12804
                     /locus_tag="PK513_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO81324.1"
                     /translation="MILRRGENIISQQNGFTLVEMAIVLLIIGFAMSAVAIGLDTYNR
                     DLRSNKTLESIRKANEQLVYFAKIFGYPCPADPTLGPGDAMYGRADCNVANGNIKNVL
                     GRDNMNVRVGAVPFADLADFSANLALSNERYVESDGFDGWDNYLTYAVTFDLTDEPGR
                     PPFDETMGAICVVDENNRSLLDEACTAHAVVISHGANGLGAYVRSGVSAPGESCIDLL
                     LPAADQADAANDPSPNEKENCDHQIGGALADAKFVAGVWNESDDESYDDVLRFIIFQS
                     REIWARGGDLVIPFGDPNDPTDDIRIPRVSSVNDGAIGIGLDAGNTPQQALHLQGDLQ
                     ADRVYVEELCDVSGGNCFDPELIGGDVADMQCPPGQVVQKIGRVSAGADPGVVCVNPF
                     TSMPFTQCPTGQVVIGVSSVSGSICGTL"
     gene            complement(12861..14219)
                     /locus_tag="PK513_02345"
     CDS             complement(12861..14219)
                     /locus_tag="PK513_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81325.1"
                     /translation="MNDPLPATEHIFNALRRSIMGEFEDLNITFIPYADGDIEAAFEA
                     KKRELREHPAGTKLLYRIEKKLSTDPQNSFFAALADHKSSKALGILKKHSVIAACFIN
                     QHDLERFEDLNTACKFIGYSTAFEAIHAYLGLLHPPGNEKQQKKTPSSPASESLRTHL
                     KGQAFAVMIMESSGEKGTLRTLLKALCEYSTTPSLYFEPEKNPLPLAADGINVVYKDL
                     KDEIPPKTGPLEHTHYMAEEIGNTYDDISLRQWIRFCYGAQEMAWAGTSQNDILSAAV
                     YGSDTPHIRSYAHICAEMLNMTPVPLKNNEIYNPFTEDSISERLHIRACKRAFAEVRE
                     AVEEQNNPALFLQKAREQTRALLKGRPLGWCAPALIEAENAYRLFKESRTAEEEIIDN
                     AFEASFSQIKWRDIKKLNRKFIAYRRSDQILTATAALELIGKDETYAPYKNAFEILNN
                     GS"
     gene            14389..15249
                     /gene="fabI"
                     /locus_tag="PK513_02350"
     CDS             14389..15249
                     /gene="fabI"
                     /locus_tag="PK513_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOO81326.1"
                     /translation="MDAQNNFLKGKRGLIMGVANDRSIAWGIAEAAAQSGAELAFTYQ
                     GEALEKRVRPLAEKIGAKIVVPCDVTDEASVDAAFKAIEDEWGQVDFVVHAIAFSDKN
                     ELDGLYLDTTRENFIRTMDISVYSFTSVAQRAVPLMKEGGSLITLTYYGAERVMPHYN
                     VMGVAKAALEASVRYLAADLGSKNIRVNAISAGPIKTLAASGIGDFRYILKWNELNSP
                     LKRNVTIEDVGRSGLFLLSDLGSGVTGEVLHVDAGYHTVGMCSVDSAAETGELLASIG
                     PKKDSGEEAA"
     gene            15357..15818
                     /locus_tag="PK513_02355"
     CDS             15357..15818
                     /locus_tag="PK513_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOO81327.1"
                     /translation="MIWFKDYTLDYLEGLRNANMGTHIGLEFSEVGPDFLKGRMPVDE
                     RTTQPFGILHGGASCVLSETLGSVAAWMTIDPDLYRAVGLEINANHVRAVTEGHVIGE
                     CRPLHMGRRTQVWQTDITEEATGKRVCISRITVAVIEQGTLSAQKEAVHVG"
     gene            15839..16909
                     /gene="aroC"
                     /locus_tag="PK513_02360"
     CDS             15839..16909
                     /gene="aroC"
                     /locus_tag="PK513_02360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOO81328.1"
                     /translation="MTGNRFGTLFQYTTFGESHGPAIGVIVDGVPPRIALTPEDIQGF
                     LDRRKPGQSRFTTQRKEADRVEILSGVFEGLTTGTPIALLIRNTDQKSKDYDEIKDKF
                     RPGHADFTYAEKYGIRDYRGGGRSSARETACRVAAGAVARKVLEDQIGGKIKIRAAVV
                     QIGEKKINRDRWGWDQVEQNPLWLPDAEAVPEIEAYLDGIRKSGSSAGALVEVHASGI
                     PAGLGAPVYDKLDADLAKAMMSINAVKAVEIGAGFEAVSLRGEENADEIRSDGFQSNN
                     AGGILGGISSGQDIVVRVAFKPTSSILTPRKTIDIHGEETDIITKGRHDPCVGIRGAP
                     VCEAMMACVLADHWLRHKAQCL"
     gene            complement(16904..17803)
                     /locus_tag="PK513_02365"
     CDS             complement(16904..17803)
                     /locus_tag="PK513_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO81329.1"
                     /translation="MGSLLFRNGRTKDAEAIFRQHVDLTPDDLKARQTLGDIYRVLGQ
                     DRNAETQYKSILNIDPHDTYALNSLGKLEMRRGHFDKAESYLQTALQHHPHNAPTLNL
                     LGNLEMQRGNFDKAESYLQTTLQHHPHDEMALNSLGNLEMQRGNFDKAESYLQTTLQH
                     HPHNSPAYYGLAKVAWLKNDKSTCQKIIKEQMQNNRASIPQIILFLACKFEQEPLLPI
                     LKEQYGEKSVENMLELAHRFESDIFAERTWPAMLNPLYAHPELYTPVNGRTVLEMGDV
                     PDGIRQATAQTVTLRHEAKDCAL"
     gene            18213..20063
                     /gene="sppA"
                     /locus_tag="PK513_02370"
     CDS             18213..20063
                     /gene="sppA"
                     /locus_tag="PK513_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOO81330.1"
                     /translation="MAQPKMQWKILPILWLALKRMAMGIGFLVLLNLLVALIVMPAFL
                     PKKAAAPSLPSEMVLFMKFEDGLRELPSPPSFADPFAGASSATVHDIVNALDYAREDE
                     RVKGIIARMYAGNFEFSKITEIRAALKRFRATGKFAHIYSSSYGEGGGGLSRYYLASA
                     FDEIWMQPLGVVSIPGVGLEVPFARGTLDKLGISPQFFQRKEYKTAYESLTNKAMSAP
                     NREELGAIVNDIRFEMLEQIPADRGMSEAAFEKLVNKGLFTAPEAEEAGLITHADYGD
                     VLLDNVAELITGERDADGLELIDLKDYGRVMEKKAHGLMVGEKAKVALVYAVGAIMPS
                     GDGGGFGGDGIAAADEIAPAILKASKDEDVEVIVLRIDSPGGSPSASESILRAVEKAR
                     ERGKPVIVSMGATAASGGYWIAAYADKIYALPTTLTGSIGVVGGKFALGDLFGKLGVN
                     WEKIHWGKNAGLWSANTPFSASEAERINAMLDNVYDNFIARVAKGRGMEKDAVDKVAG
                     GRVWTGRRALEAGLVDELGGLEDALDYAAELAKVESRKDLNVVVYPKPKTPLEQILEL
                     LDTQSGVISQMGRAAAWQERFFEAAEPALELLQVHENPQDYIVYEALQIR"
BASE COUNT         4471 a         4110 c         5845 g         5899 t
ORIGIN      
        1 cgttacggca tctgtgggaa accaatcacc agcttatgca gattgcggct taaatcacct
       61 ttgccggacg cgaggtgacc gcttcgcgga cgtaatgttt tctagccaag ctttgcttga
      121 agaaacccta aaaagcccgc tggtgggcgg gctgtttttt caaattatat tcggttttat
      181 gcgctggtgt tgatattggt gccgcgggtg ctggaaatgg gcgcagagcg ggcggcatca
      241 tcaaggattt gcactaaagc ctgcgcttga tctgcggatt gcttgatcac ggacagggcc
      301 acattctgcc tgagaatggc tcgttctgcg gctatccctg cgggcactgc tgtcataggc
      361 gaatcctttc cttcctacct cttattatcc tatcaaaaaa ttaagattcc ttaaagtctg
      421 aagagagggt tttttaagtt ttgtattttt tgaaatagcg ggtgcaggct ttgtgaagct
      481 cttgtaccaa ggtatagcct tccgggccgc catcaccttt gagatcgttt ttgatgatga
      541 tttgaatggc attttcatga gctttaatga tttcaatggc ttcggtattg tactcatgga
      601 cggcttccat aaactgcagg caaatgtcag cgacgtctgt gaggaggcaa tatttaaaca
      661 tacctccatt ggctttaagt tgcatgaccg ggtggatcat tttttcctta ttgaaaacct
      721 ttttgctctt gatgctctgg atggcatcat ttgtggcttt gtcgagagtt atcgcgagtt
      781 ttctggcttc aggacggaag tccatttcaa aattattgat gacttcttgt gcccgttgga
      841 tacgttcttc agaaatacct ccgccgccga ctttcatttt tagtcggttg ggaggggtga
      901 tgaattttac gttttgtgtg tttttcatag tttactcaga ttttttgtgc ttcttcactt
      961 aatttcttta aaatgttcag agcttcgggg gtgttttggg tcggtgtgtt caccgggtct
     1021 tcatcgcggc ggcgtggacc ttcgtagccc ttgccgcttt tacggcggcg atcggggcca
     1081 aaaaattctc ctgtatctac gaattttctg ggtttttcta tgatctggaa aactctggca
     1141 tagagatcct gcgccgtaaa aggtttgact aaaaattctg tgattccatt gtcccgcgcg
     1201 ccttcaacgc gaagctttga gctgaatcct gtcatcataa taatagggac gtaagggtta
     1261 ggggacctgg ggtctgtgcg aactttttta gtgaactgga ggccgtccat cggttccatg
     1321 atccaatcgg ttatgatgaa gtccggatcg tgcttgcaaa gcaggttaaa ggcttcttct
     1381 ccattttgtg cggtgaaaat atgagtgaaa ccgaaggttg ttaagatgct tttcgttaat
     1441 gtcagcatag gcttcatatc atcaacgagt aaaattttaa catcattaag attgtaaggc
     1501 atatgattcg catgctctcg ggttattggt gaatatcagt aaagtctgtt taacgtactg
     1561 aattacttct cagttttcat tctagcacag ccaagtgttt accccaaatt atttaaatag
     1621 gctttaaaac ggtgatgaac atctatggca ttcatgccca ggtccggagg aagttttccg
     1681 ggggtgattt ccgcgtggat gaaatgcggg ccggacagca ggtcaaggtc tttcatgtcc
     1741 ttgagcgcta tgttgctttg aaagcccgga caggccaatg atatgtggtt ccagtttata
     1801 cctctggttg gtgtaggaag tgattttgtg aattcgtaga tgctattatc catcacaact
     1861 acgctcaggt tttttggcgc tgcattggat attgtggcca gtgagctgag attcatgcct
     1921 agtgcgccgt ctccgcaagc ggcgatgacg gcgcggtctt tacatgccat tgctacgccc
     1981 agggctacgg aagaggccat gcccataggg ccggagaggt agaggttttc cgggcggtct
     2041 ttggcttctt tcagccagag agattgtgag ccaatatcgg caacaatcag agcattgcgg
     2101 tcttcggcga tctttgtgag cctttcaaat gcttcggatt gtttcatgcc tttagccctt
     2161 ctgagagggt gatgaaggct ggctcgcctg cgccttccga gcgtgcgatt gctttgggaa
     2221 tgttgagtgg caaattttcc cacgatgagg gataaacgaa tatattcact gcccgcgtaa
     2281 gtgcttcgaa acttttatat ttattggggt catagctgtt tgaatcgttg attccgccgc
     2341 gatgggcaat caggcagaca aacggcgttt ggtatttttg gcaaagcgtt gtgaaggcgc
     2401 ctacagagtt caggaagccg gaattttgca ttaaaaccaa agcgcgctgt ccgctcatgg
     2461 ccgcgcctgc ggcgatgcca aaacattctt cttcgcgtgc ggcggggatg taatcgaaac
     2521 gtttgtcttc gctcagtccg ttaatcatgg gtacaagcca gccatcaggt acgccagtgg
     2581 ccaagtttat gtcgtgttta tccagagcgt ctagaatggt tttaatcatg gtttcatact
     2641 gctcaatctc ttttgtcatt gcaatagttt gtccagagat aatgcgtttg attcggcggg
     2701 gtatgtggat caattttttc aattgttgtg tggggcattg tccgtattcc tttttgagcg
     2761 tagcatgaag gcattaaaga atcattgtgg ttttacggcc ttcttcacgg atgttttgtg
     2821 atatttgcgt gaggagttgg ccgagttcca tgagaggcaa agccgatgag tgcaaaacaa
     2881 atcattatga attttacggt gccgccaagt attgatgatc tcaatgtaat ggcagcttct
     2941 attcttgaat ctcttcccga agaattgctt gaattttgcg acagtctggc gatttcgctg
     3001 gatgaatttc ctgatgaaac gttggaacag gagcttgagt tggaagatac atacgaattg
     3061 ttggcgctct atcgcagtgg aaaggagatt tctcccggtg ttgagaaaaa aacggcgaat
     3121 gatgaggatg tgttggttct gttccgtcgg cctattcttg atatgtggtg tgaaatagga
     3181 gaggatctgg gcggtttatt gcggcaagtt atgattgagg agcttggtaa gtatttcgat
     3241 ttttctgaag atgagattga agaaatgagc gagcgtcatt atcagggcat gctttagtcc
     3301 tttacatttt tctgcagaat tgagtgtgcg tggtattttt gttcttctgg gactcaattc
     3361 aaattatgca gttttatcaa gcttgtgata gactatagaa ggtatgatat ttaaaattat
     3421 gtgagtttgg gtttggcggt gcggtcaatt ttagcacttt ttgtagtatt atcaatactt
     3481 tgggtgccga ttcatgtgcg cgcggcggcg gctcccattg aagagtctcc gggctgtgat
     3541 cctgagtttt gggatgtttt ggaggcgcgt acctggctgg aggggcagcg tgaggtcgag
     3601 gcggcttcgc gtcttattct caaggctgat agtgtcttac aatatacttg ttttaatgac
     3661 cggattagtg agttggcttc ggcggctgac tggattttta gtgatcgggt ggttggcgct
     3721 cccttgttta caaaccatcc gatgtggagg cagcggcatc ctccgccacc aggggtggcc
     3781 ggagcttcgc cttttacgcc tatttacctt ggtccgaatg gccctgatcc gccgggaggg
     3841 gcgttcacgg cgcttgagct tgacaagcat ttgaacaatc ttgtgcgtgg gccgatgaat
     3901 acctatctcg gaaataattt taatcatact tatggtggcg gtcttgggcc ggtcggcggg
     3961 gtttgtatgt cgatgaacac tgtatggaat tttctgagat gtcaggatta tgataaggat
     4021 ctgtttataa gccttaagga tatgccaggg aatgatccgc gaactttgcc ttatacttgt
     4081 ccgaatgccg gggcgagaac ggcgaagtgg aatttgaatt tgctcaaggc gttcccgccg
     4141 ccgtctcttc cggctgcgct gggggggatg gatttgaaag agcattacaa taatcgaatg
     4201 aataataatg gcggctgcag cggtgcgttt ccgatttcga ccggggttgt tgtgaaaatg
     4261 ggcggaggga atcatcctga tgctgtttgt tctatcccag ggtgttctta tgatggaagc
     4321 ggtggttgct tctaatccag ccctttgctt ataaaaccgg ccatacggcg ggcgaaggcg
     4381 ccgggatctt ttacgggctc gccctggatg attttggctt gatcaaaaag caggaggcct
     4441 gcgtttttaa agtcttccga ttttgctttg agtgtttcca gcttttgaat gaggggatgt
     4501 tcggcgttga tttccagcac aggttttgac gtgcctgtgt atttttgatg gatcttaagg
     4561 acgcgttcca tatgcatatc gacgccgcct tcgggggcga cgaggcacac gggagaatct
     4621 gtcaggcggt ttgaaatgcg tacctcggaa acatcgtctt cgagcagttt tttaaggttt
     4681 tcaagcaggt ctttatgtgt ttcagtgctt ttttcgtctt tcttttcgtc tttgtttttg
     4741 cccaggttaa tgtcgccttt ggtgacggat ttaaaactct ttcctttgta atcgcttaca
     4801 ttttgtagcc agaaatcatc aatggtgtcg gtgaggaaca agacttcgat gcctttggct
     4861 ctaaagcctt cgatttgcgg agagttttta agggactgca aattttcacc gctgatatag
     4921 taaatatctt tctgatcttc tttcatgcgc tcgatatatt catcgaggga tgtaaatttt
     4981 tcgccccctc cttctttaga gtcgtgggtg gagtaaaagc ggcagatttt gaaaatggct
     5041 tcacggtgct gctcggcgtc atacagccct tctttgatcg ccgggccgaa ttgtccccaa
     5101 aaggcgctga agccaattga atcatcgcgg gagagcttat caagatcact caaaatatgt
     5161 ttggttacgg cggtgcgaat gcgcgtaatg attgggttat attgtagaga ttcgcggctg
     5221 atattgagag gcaggtcttc tgagtctata acaccgcgca cgaagcgcag ccagggatag
     5281 gtcaggcctt cgacattatc cgagataaac acccggcgca catataggcg cacggcattc
     5341 ttgcgtgaag ggtcgtacag atcccacggg cgtaaggttg ggataaacat cagggcggag
     5401 aattcgatct tgccttcggc gtgccagtgc gaggtcatga gcggttcgtc aaaaccgtgg
     5461 gtgatgtgtt tataaaattc gtcatattgt tctttggtga cgtcattttt ggagcgtgtc
     5521 cagatggcgc tggctttatt tatcggctgg ccttcgccag tatcggtatc ggtggggctg
     5581 ccgaggaaga ttttaaaatc gatatgatct gaataggttt caaccgtttg tttaattttt
     5641 tcgttgatga ggaattcggc tgcctcgtct ttaatgtcga gaataatggc agtgccgcga
     5701 tccccatcga gccgcgcttc ttcgtccttg gaggctttgc ggacgttaaa gccgctcgcg
     5761 ccgtcggatt cccaaaccca gatcgatttt gaaccggctt tgcggctgat aacgcgcaca
     5821 tggctggcga ccatgtaggc ggcgtaaaag ccaacaccga attgtccaat caatttaagg
     5881 ggatcgccgc cggatttttc tccggccttc attttttcca tgagggcggc tgtgccggat
     5941 ttggcaattg tgccgagatg gtcgatcagt tcggatttgc tcatgccgat gccgttatcg
     6001 accacggtga gtgtgcgggc gtcggtgtct ttgtagatat gaattctaaa ttgcggattg
     6061 tcggcggtga gttccggttt ttcaatggct gcataacgca ggcggtcgca agcatcggcg
     6121 ctgttggaaa tgagttcgcg caggaagaca tcatgattgg tgtaaagcgc gtgggcgacg
     6181 atatcgagca ggcgcgaaac atcggcgctg aaactgtggg tgttgggcga tgggtcttgg
     6241 gttttgggct gggcgttcat ctttttactc tctaaaaaac gtcatgcccg ttttatacgg
     6301 gcatccgtat ttccgcacaa ggcggggatg acacataatt tgtgcatcac gggcacatcc
     6361 aatgtgcatc ccactggccg tccttgtttt tgcgataaat aaaacgggta tgcaatcggt
     6421 gtttgttcgc aatccagaat tcgattgaag tcggattcac gcggtagcct ttccaatatg
     6481 ggggcctcgg gattttatcc agatttgtga atttcttgac gaattttgcg actgcattgt
     6541 ccatgtggtt ttcatcgtcg aatgtttcgg attgctgcga agcccaggcg ccaatctggc
     6601 gttctttcga gcgcgtggcg aaatattcgt ccgtctcttc atcgctgacg gggcggatcg
     6661 tgccttctat gcggatttgt ttgcgggtgg atttccaata aaagcacagc gatgcatagg
     6721 ggtttgcttc cagttcctga cctttgcggc tggcgccgtt ggtatggaat ttaaagcctt
     6781 catcgctgat gtctttaatc agcaccatac ggtttgaggg gcggccacgg gcatcggcag
     6841 tggcgagatt aacggcttct gggtcgttga tttcactttt tgtggcttcg gcgagccagt
     6901 ctttgcaaag agcgatgggg ttggggccgt atttctctat gatcttattg ctcattttta
     6961 ttcgctcttt ttttgatttt tatcaaaggg tttctatttt ataaacgtat agtgtcttgc
     7021 atgtatctaa atacgatata tgctgcgtgg atacgaatcg caagatgaag aaaggcgaag
     7081 actatgaaga atttattttt aattcctttg gtgctggtgg cgtttgttgc gagcggttgt
     7141 gagaacattc aaaatagcgg cacgaaagaa aaaatcggcg gcgtgtccgg cgcggttgta
     7201 ggcggtttgt tagggtctaa agttggcggc gggagcggcc agctctgggc gaccggtgcg
     7261 ggggctttgc ttggtgcgtt cattggtagc gagattggcg catctttgga taaagcggac
     7321 cgcgttatgc tggggcaggc caatcaacgt gcgcaaacgg cgccgattgg tgagtcggtc
     7381 tcatggaata atcctgaaag tgggaactat ggtacggtaa cgcctgtgcg tgacgggcaa
     7441 agttcttatg ggcgttattg ccgtgaatat gaacaaatga tctatgtcgg cgggcgtcag
     7501 gaaacggctg tcggtacagc atgtaagaat gatgatggta cgtgggaagt tgtcagctag
     7561 tacctgtctc ttgctatcct tattaaagta aaaaagctgc ctcccatgag acagcttttt
     7621 tatatttccc ctttgaaaat tatcatattt acaatttgat aagtacaaaa tttacacatt
     7681 gagaagattt tgtgttggtt gctgtaattt tttttgaata aaaaatcatt atttatcaat
     7741 gtgttatatt tttatttgtt atcaatataa aattttaact aattttaact aatttttgct
     7801 agtcttggta agagagggaa aatttgtctg ccttgaggta tgtggaatca aatacgtatt
     7861 taacgttgta gatgaatacg ggtgaaaatt aagaatatgg ggcgtaactt tggataacaa
     7921 atttttacaa cacaagattt taaagtctga aaacggtttc acactgattg atgttgcggt
     7981 tgcgttgtta gttattggta tattggccgc tcctcttgtt cagcaatatc gtatttggct
     8041 tttgacatcg aagcagcaat tgacggcggg cagagctcaa aatgcaagag atgccattga
     8101 gcaatattac tttgtgaata accgttatcc ttgtccggca gaccccagac ttggtcctag
     8161 cgatgctgat ttcggcgtgg aattgcgcga ttttggcgga accgggcagt gtgatacgag
     8221 cgcaacggcg ctgggtcctt ttacagttac atcggctttg gatgcagatg gcgttggcgg
     8281 taatgatccg gtttatatgg ggatgctccc ttttaagtca ttaagtttat ctccccatga
     8341 agctcttgat tcctggaata ataaattgac ttatgcagtg tccggtttgc ttacaaatga
     8401 ggctacattt gccggcatat tcggtggtat aaccatccgt gaagattttg tgatgcggca
     8461 tcctacagtg cttgacgcaa ctgcagatcc tgcggcggat aatgtttgta cggatacaag
     8521 cctgcttgtt cccggcgcgg ttccaccggc tcaggtctat ggtccggtta ataatgttca
     8581 ttatgtcatt ttctcacatg gcaaaaatgg caatggcgcg tattacaatg ctaataacgc
     8641 tgcgcctgcg cgagcgtgtg atatggccgc tgcagatgcg gaaaattgta acaatatcac
     8701 gaataacgag gcgacttttc tttatgatac ctgccaatcc tcgagtgcga gtgatgcgaa
     8761 taactatgat gatatttttg ttgccaataa tataacgctt agcagtgcgc catttgcaaa
     8821 ctggaatcct gctgtggacc agcaaaatgc aggggcgccg actggatttt atggcattaa
     8881 taacgctctg ccggaagcgg agcttgatgt tatcggtaat gttcgcgcca gccttgatcc
     8941 gttagattct caagaactgg gccgtttgta ctctcatcag tattgtgacg ggaacggcgg
     9001 aaattgcatg aagcccgagg cgattgcggg cgaagagcct gatatgcgtt gtggtttttc
     9061 aggtacggct atgagggggg tcgctcataa tcaggtgcgt tgcaatattg ttgtgcaaac
     9121 cttgagttcg gcgacatgtc cgggggcgcc aggaccccag caatatgtga caggtttttc
     9181 agcgtctggg caaccttgtt gcaataacgt atgtccttaa gagaattgag taaaaaaatg
     9241 tggagaaagc aaatgaatgt gcaaactaaa acaactcgca agccttcttt cccggcccgc
     9301 tacagcgagg gtggttttac gattgtggaa atggccattg tcaccatgat aatagggctg
     9361 gttttcgctg cggcttttca gttgcttgag caaaggcagc gttttgttcg cgcggaaaat
     9421 acgaaaatag ctgtttcaaa tgcgaatgta gcccttgcag catttgttaa taattttggg
     9481 cgttaccctt gcccggcttc tttgaatgca acgcgggata ataatggacc tctctacggt
     9541 gtggagagtg attgtacaga tactggggct gttgctgcgg gtagcaatgt tgatgcggcg
     9601 cttggttatg ccgttgttac gggcgcgcgt aatattgcgt atttcgatcg ttcgacagtg
     9661 ccgcctacgc gggttcctgc tgccggcatt gcgcctgtgc gggtgcgtat tggcgctttg
     9721 ccatttaaaa atctgaattt gcaggagggg caagcttatg atggctatca gaaccggttg
     9781 ctttatgctg ttgccgaacc gctggccgtt ggaaaaacat ttagcgccgg gggaggggtc
     9841 attgatgtgc aaaatgaact tgatgcatct gttttacagc cgcaagcctc tgcgcatttt
     9901 attattttaa gtgctggtga gaatggtgcg ggagcttatg gttatgaagg acgtagagct
     9961 gcctgtctgg ggacaagcga ggaatctcaa aactgtgatt tcgatgtaga tgcgattttc
    10021 cgtgttgcag agaccgggac ggataataac ggcgcggccg ctacttatga tgatttggtt
    10081 gtttatgctc ttgatgggga tattccaatg tgggagctgg aaggtgcaaa tgatgatgcc
    10141 atccttaaaa ctgtcgggga tatcggtatt ggcagcgttg ttgcagcgaa taaccccggt
    10201 gaagatttgc aggttgacgg gactttgatt gtccgggaca atccgggtac gtcagagctt
    10261 gagggaaagg tcgaatctga agagatttgt gattttacca gcgggagcac aacatgcttc
    10321 aaatctgagg ttattgccgg tgatctatcc catgatcagg gactcatttg tggtccaggc
    10381 ttgtttatga caggaattca aaatggcgct ccggtttgca ctgatgaggt cattgtgacg
    10441 tgtcctgccg gaaaatttgt tacgggggtt gattctgacg gagggcttat ttgtgatgct
    10501 acgccgccac cgtcttgtcc ttccgagagt aaaaatctgt gttcaggatc gggacttctt
    10561 cctagcgccc cgcataatac aatagagact ataaccgtat cgactgcgcc gacgaatcca
    10621 ggcgggacgc gcagcgccaa gtatagatgc aattccgggg tgtgggagta tcagtcctct
    10681 tggggagatc cgtgtaattg tacgcctgtt atcgaccccg ttgttgtgaa ttccaaagcg
    10741 tcttgtcatg atggcaacaa ctgtaacggt agttttaccg gacatgttgt cgtgacttca
    10801 acaacggcct gtccgagcgg tctttcgacg caggtaacag accggtctgg ttgcgggtgt
    10861 ttgaccggga cgaagattac gcctgtaggg tgtccgtctg gttttaataa ggggcgcaag
    10921 attagaagct cgacttatgt ttgtaatgtc agcggagacc tgacgcgagg ggcatgcact
    10981 tcgccgacac cggcggccga cgatcctgat gatccttgcc gttgtgagcc tgttcctcct
    11041 tttctttggg cttatgctcc ttgccctctt ggtttgaagg cccttaatga taaaggcatt
    11101 ccacaaaacg cggaattcga gtgtagtggc ggttcggatg ctcctggagc atgggtcatt
    11161 tatccgaata agaacgatcc tgcggttatc gctcagtatt gttcatgttc tgtaccgccg
    11221 ccttctaatg taacgcgcag tgattgtccg cccgggtttt ccggtgagcg tacctacacg
    11281 attacaaaga cgtgcgatat tctccatccg ttggatccgc cgactgtgac cgaggagatt
    11341 tataaagatg attgtttgcc tgatgctgtg ggcatgggca agtgggaggt cgtggggggg
    11401 gcggctggcg ctgtaagccc agttgttcac gtggggggga catgtattct tggtactgat
    11461 tcgccgcctg ccaggttatg taaaaaagtc catggtcatc atgttaaagc ttgtttgtgc
    11521 caataatgaa aaggaacgat catgattttg cgtaggggtg aaaatataat ttcgcaacag
    11581 aacggcttta cgcttgtcga gatggctatc gttttgttga ttatcggctt tgccatgtct
    11641 gctgtcgcta tcggccttga cacgtataac cgggatttac gttccaataa aacgcttgaa
    11701 tctatccgga aagcgaatga gcagttggta tactttgcaa aaattttcgg ctatccatgt
    11761 ccggcggatc ccacgctcgg gccgggtgat gccatgtatg ggcgcgctga ttgtaacgtt
    11821 gcgaatggga atattaaaaa tgttttgggc cgcgacaata tgaatgttag agttggcgcg
    11881 gtgccgtttg ccgatcttgc tgatttttct gcaaatctcg ctctttcgaa cgaaagatat
    11941 gttgaaagtg acggttttga cggctgggat aattatctga catatgctgt tactttcgat
    12001 cttaccgacg agcccggcag gccgcctttt gatgaaacta tgggcgctat ttgtgttgtc
    12061 gatgaaaata ataggtcgtt gctggatgaa gcctgcacag cgcatgccgt tgttatttcc
    12121 cacggcgcta acggacttgg cgcttacgtg cgttcggggg tttcggcgcc gggcgagagt
    12181 tgtattgatc ttttattgcc tgctgcggat caagctgatg ctgctaatga tccctcgccg
    12241 aatgaaaaag aaaactgtga tcatcagatc ggcggcgccc tcgcagatgc caagtttgtt
    12301 gcaggggttt ggaatgagtc ggatgatgaa tcctatgatg atgtgctgcg ttttataatt
    12361 tttcaatcaa gagaaatatg ggcgcggggc ggtgatctcg ttatcccgtt tggcgacccg
    12421 aatgacccta cagatgatat tcgcattccg cgtgtttcgt ctgtgaatga tggcgctatc
    12481 ggtattggtc tggacgccgg aaataccccg cagcaggcgc tgcaccttca gggcgatctt
    12541 caggcagacc gtgtttatgt ggaagagttg tgcgatgttt caggtggtaa ttgttttgat
    12601 cctgaattga ttggcgggga tgttgctgat atgcaatgtc ctccaggtca agtggttcag
    12661 aaaattggtc gcgttagcgc gggtgcagat cctggcgttg tatgtgtaaa tccgtttacg
    12721 tccatgcctt ttacgcaatg tcctaccggt caggtcgtta ttggtgtttc cagcgtaagc
    12781 ggttcgatat gcgggacgtt ataatggcgt tataaacagt ataaaagccg ccttttgatg
    12841 ggcggctttt gtatgcgttg ttatgatcca ttgtttaaaa tttcgaaggc atttttgtaa
    12901 ggtgcgtagg tttcgtcttt gccaatcaat tctaatgcgg cggttgcagt caatatctga
    12961 tcgctgcgac gataagcgat gaattttcta tttagttttt ttatatcgcg ccattttatt
    13021 tgcgaaaagc ttgcttcgaa tgcgttgtcg attatttcct cttcagcggt gcggctttcc
    13081 ttaaacaagc gataggcgtt ttcagcttcg atgagcgctg gggcgcacca gcccagtggt
    13141 cttcctttta acagagcgcg ggtttgttcg cgggcttttt gtaaaaacag ggccgggtta
    13201 ttttgttcct ccacggcttc acgcacttcg gcaaaggctc gtttgcaagc gcggatatgg
    13261 aggcgttcgc tgatggaatc ttctgtgaag ggattgtata tttcgttatt ttttagtggc
    13321 actggcgtca tattaagcat ttcggcgcag atatgggcat aggaacgaat gtgaggggtg
    13381 tcagacccat acacggcagc gctgaggatg tcgttttggc tggttccggc ccaggccatt
    13441 tcctgggcgc cgtagcagaa tctgatccat tggcgcaggc taatatcatc ataggtgttg
    13501 ccgatttctt cggccatgta gtgggtgtgt tcaagcgggc cggttttggg cgggatttca
    13561 tctttcaggt ctttatagac gacattgatg ccatcggcgg cgaggggaag agggtttttt
    13621 tcgggctcaa aatatagtga aggtgtggtg gagtattcgc acaatgcttt gaggagggtt
    13681 cgtagcgttc ctttttcgcc actgctttcc atgatcatga cggcaaaggc ttgtcctttg
    13741 agatgtgtgc gtagagattc gctggcgggg gaagacggcg tttttttctg ctgtttttcg
    13801 ttgccaggag gatggagcag gccgaggtaa gcatggatgg cttcgaaggc ggtgctgtag
    13861 cctataaatt tacaggctgt attcaggtct tcaaaacgtt ccagatcgtg ttggttgatg
    13921 aagcatgcag cgatcacgga gtgttttttg agtatgccca aggctttgct tgatttatga
    13981 tctgcaagcg cggcaaaaaa actgttttgc gggtctgtag agagtttttt ttcgatgcgg
    14041 tagaggagct tggttcctgc gggatgttca cgcagctccc gttttttggc ttcgaaggcg
    14101 gcttctatat caccatcagc ataagggata aaagtgatgt ttaaatcttc gaattcaccc
    14161 atgatgctac ggcgcagggc gttgaaaata tgctcggtag cgggcaaggg gtcattcatg
    14221 agatataaat gcggtagcga gtgaaaattt cgatcttggt gatattatca ggaaagggcc
    14281 atgataccaa ggtggtgatt tggatgaggg ctggacggat acttcgaaat gtgtaggatg
    14341 ctgctatcgt aaatttattc aatattattg atgaagtgag taatgactat ggatgcacag
    14401 aataattttc ttaaaggcaa gcgcgggttg attatgggcg tggcgaatga tcgttcgatt
    14461 gcctggggta ttgccgaggc ggcggcgcag agcggtgcgg agctggcttt tacctatcaa
    14521 ggtgaagcgc tggaaaaacg ggtgcgtcct ttagcagaga agattggtgc aaagattgtt
    14581 gtgccgtgcg atgtaaccga cgaggccagc gtggatgcgg cttttaaggc tattgaggat
    14641 gagtgggggc aggtcgattt cgtcgttcat gcgattgcgt tttccgataa aaatgagctg
    14701 gatggattgt atctggatac gacgcgtgag aattttatcc gcacgatgga tatttccgtt
    14761 tattccttca caagcgtggc gcagcgggcg gtgccgctca tgaaagaggg gggatcgctc
    14821 attacgctga cctattacgg ggcggagcgc gtgatgccgc attataatgt tatgggcgtg
    14881 gcgaaggccg ctctggaggc ttctgtgcgg tatctggccg ccgatctggg gtcgaagaat
    14941 atccgcgtga atgcgattag cgccgggccg attaaaacgc tggcggcttc gggaattggc
    15001 gattttcgct atatcctgaa atggaatgag ttgaactcac cgctcaagcg taatgtaacg
    15061 attgaggatg tggggcgcag cggtttgttt ttgctgtccg atttaggtag tggcgtaacc
    15121 ggcgaggtgc ttcatgtgga tgcgggctat cacaccgttg gcatgtgcag tgttgacagc
    15181 gcggcggaaa ccggtgagtt gctggcgtcc attgggccaa agaaagatag tggtgaagaa
    15241 gctgcttagt tttttagtca ttgcgaggag ggcttgtagc agccagatgc ggcaatctat
    15301 accctctgga gcacaagtgg attgcttcgc tacgctcgca atgacggagg aggaaaatga
    15361 tctggtttaa agattataca ctcgattatc tggaagggct gcgcaatgcc aatatgggga
    15421 cgcatatcgg gttggaattt agcgaggttg gtcctgattt cctaaaaggc cggatgccgg
    15481 tggatgagcg cacaacgcag cctttcggta ttttgcatgg cggagcaagc tgtgtgcttt
    15541 cggaaacgct gggcagcgtg gcggcgtgga tgacgattga tcccgatctt tatcgtgctg
    15601 ttggcttgga aattaatgcc aaccatgttc gcgccgtaac cgaagggcat gtgattgggg
    15661 aatgccgccc gctgcatatg ggccggcgga ctcaggtgtg gcagaccgat atcaccgaag
    15721 aggctactgg caagcgcgtg tgtatttcac ggattaccgt cgcggtgatt gaacaaggga
    15781 cgttgagcgc gcaaaaagaa gctgttcatg tcggctagag cgggttagag taacggctat
    15841 gacaggtaat cgtttcggaa cattatttca gtatacaact tttggtgaga gccacggccc
    15901 ggcgattggc gtgatcgtgg acggtgtgcc gccccgaatc gcgttaacac ctgaggatat
    15961 ccagggattc cttgatcgcc gcaagccggg gcaatcgcgc tttaccacgc aacgcaaaga
    16021 ggccgatagg gtagagatcc tttccggcgt gtttgagggc ttaacgaccg gtacgccgat
    16081 tgctctgctg atccgtaaca cagaccagaa atccaaggac tatgatgaga tcaaagacaa
    16141 gtttcgtccc ggccatgcgg attttactta tgctgagaaa tacggcattc gcgattatcg
    16201 cggcggcggg cggtcttcgg cgcgggaaac cgcttgccgg gtggcagctg gggctgtagc
    16261 gcgtaaggtg ctggaggacc agatcggagg caagatcaaa atccgcgccg ctgtcgtgca
    16321 gatcggtgag aaaaagatta accgtgatcg ctggggttgg gatcaggtgg aacaaaatcc
    16381 tttgtggttg cctgatgccg aggctgtacc ggagatagag gcgtatctgg acggcattcg
    16441 caaaagcggg tcttctgccg gggcgctggt cgaggtgcat gcgagcggta ttcccgccgg
    16501 tcttggcgcg cccgtgtatg acaagctgga tgcggatctg gccaaggcga tgatgagcat
    16561 caatgcggtc aaggcggtgg agattggcgc aggttttgag gctgtttctt tgcgcggcga
    16621 ggaaaacgcc gatgaaatcc ggtccgacgg gtttcagagc aataatgcgg gcggcatact
    16681 cggcggaatt tcttccggac aggatattgt tgtgcgcgtc gcgttcaaac cgaccagctc
    16741 gatccttacg ccgcgcaaga ccatcgatat acacggcgaa gaaaccgata tcatcaccaa
    16801 aggccgccac gatccatgcg tcgggattcg cggcgcacct gtttgcgagg cgatgatggc
    16861 ctgcgtcctc gccgatcact ggctccgcca caaggcccag tgtttatagg gcgcagtctt
    16921 tggcctcgtg gcgcagagta acggtttgcg ccgttgcctg tcttattccg tctggaacat
    16981 cacccatttc caaaactgtg cggccattta cgggggtata aagttcagga tgcgcgtaaa
    17041 gggggttcag cattgccggc catgtgcgtt ctgcaaaaat atcgctttca aaacgatgag
    17101 ctagctctaa catgttttct acactctttt cgccgtattg ctctttcagg attggcagta
    17161 gcggttcctg ttcgaattta catgcgagga acagaataat ttgtggaatg gatgctctat
    17221 tgttttgcat ctgttccttt ataatctttt ggcatgtgct tttatcattt tttagccatg
    17281 ccactttggc tagcccataa tatgccgggg agttatgcgg atgatgttgg agcgtggttt
    17341 gtaaatagct ctcggccttg tcgaaattgc cacgctgcat ttcaaggttg ccaagcgagt
    17401 tgagggccat ctcgtcatgc ggatgatgtt ggagcgtggt ttgcaaatag ctttcagcct
    17461 tatcaaaatt gccacgctgc atttcaaggt tgccaagcaa gttgagggtt ggggcgttat
    17521 gaggatgatg ttggagcgcg gtttgtaaat agctttctgc cttgtcaaaa tggccgcgcc
    17581 gcatttcaag tttgccaagt gagttgaggg cataggtgtc atgcgggtct atgtttaaaa
    17641 tcgatttata ttgtgtttcg gcgtttctgt cttgtccgag aactctgtat atatccccca
    17701 gtgtttgacg tgcttttaaa tcatctgggg tcaggtcaac atgttgtctg aatatggctt
    17761 ctgcgtcttt ggtgcggcca ttacggaata acaatgatcc catatatgtt cggttttggg
    17821 gaattttgtg aaggttttgc tcctgataaa gctggtaagc gcgccataag ccatttttct
    17881 cgtaatattc ttgaaatttt ataaacgctt tagcttgttg attaaaaaag gatcgggcgg
    17941 tttctgtgag cgagcagaag tatcgttcct gtcggatatt ttgccgtctt ctttgtctat
    18001 gacccctaat gctcattttc tatgccgtta ttcatttttg aatcttttta gaatccgcaa
    18061 atcgttatgt caataaaaat aaaatatccc tattcgtaaa aatcctgttc aaaaagcttt
    18121 tcgtcatgtt tggcggacgg tatagttttt tcatgtcacg acgaaatttt agacagcatc
    18181 gaccgttttt ttctaacgct atgccgcgtg cggtggcaca gcccaaaatg caatggaaga
    18241 ttttaccgat tctgtggctg gcgctgaagc gtatggcgat ggggattggg tttctggttt
    18301 tgctcaattt attggttgca ctgattgtga tgcctgcctt tttgccgaag aaggctgcgg
    18361 caccttcgct ccccagtgaa atggtgttgt ttatgaagtt tgaggacggt ttgcgcgagt
    18421 tgccgtctcc gccgagtttt gccgatccgt ttgcaggggc atcgagtgcg acggtgcatg
    18481 atattgtcaa tgcgctggat tatgcgcggg aagatgagcg ggtaaagggc attatcgcgc
    18541 ggatgtatgc cgggaatttc gagttttcta aaatcacgga aatccgcgcg gcgctgaaac
    18601 gttttcgtgc aaccgggaaa tttgcgcata tctattcctc ttcctatggt gaaggcggtg
    18661 gcggtttgag ccggtattat ctggcttcgg cttttgacga gatttggatg cagcctttgg
    18721 gcgtggtgtc gattccgggc gtagggctgg aggtgccgtt tgcacgtggc acgcttgata
    18781 aacttggcat cagtccgcaa ttttttcagc gtaaggaata taaaacggct tatgaaagct
    18841 tgacgaataa ggctatgagt gcgccgaacc gtgaagagtt gggcgcgatt gttaatgaca
    18901 ttcgttttga aatgctggag cagattcctg cggatcgtgg aatgagcgag gcggcgttcg
    18961 agaagctggt gaataaggga ttgtttacgg cgccggaggc ggaagaagct ggcctgatta
    19021 cgcatgcgga ttatggcgat gtgctgctgg ataatgtggc tgagcttata accggggagc
    19081 gcgatgcaga cgggcttgag ctgattgatc tgaaagatta tggccgggtg atggaaaaga
    19141 aagcccatgg tttgatggtc ggcgagaagg ctaaagtggc gctcgtttat gctgttgggg
    19201 cgattatgcc gtctggagac ggtggcggtt ttggcggtga cggtattgcg gcggctgatg
    19261 aaattgctcc ggcgattttg aaggcgtcga aggatgagga tgttgaggtt attgtgctgc
    19321 ggattgatag tcctggcgga tcgccatctg cgtcggagag cattttgcgc gcggtggaaa
    19381 aagcccgcga gcgcggaaag ccggtgattg tgtctatggg tgcgacggcg gcttccggtg
    19441 gatactggat tgctgcgtat gcggataaga tttatgcatt gccgacgacg ctcaccggat
    19501 cgatcggcgt tgttggcgga aaattcgcac tgggtgatct gtttggaaaa ttgggtgtga
    19561 attgggaaaa gattcattgg ggcaagaatg caggtctgtg gtctgccaat acgccgttta
    19621 gcgcgagcga ggcagagcgg atcaatgcga tgctggacaa tgtatatgac aatttcattg
    19681 cgcgtgtggc caaggggcgg ggcatggaga aggacgcagt ggataaggtt gccggtggac
    19741 gcgtgtggac cgggcggaga gcgcttgagg ccgggctggt ggatgagctt ggcggtttgg
    19801 aggatgcttt ggattatgcg gccgaactgg cgaaggttga aagccgtaag gatttgaatg
    19861 ttgtggttta tccgaagccg aaaacaccgc tggagcagat tttggaattg ctggatacgc
    19921 aaagcggcgt gatttcgcag atggggcggg cggctgcatg gcaggagcgg ttctttgagg
    19981 cggcggagcc tgctttggag ttgttgcagg tgcatgaaaa tccgcaagac tatattgttt
    20041 atgaggcgtt gcagattcgc taaattctgc gataaacggt ctgcaaacta ctctctttga
    20101 atagacctgt tgcaaaaata ggttaattta gtaacaagcc tatttgtgaa aatattaaga
    20161 acaaccacct tcagatttga tgaaaacagg ttgatagtcg tccgtgacat ccatacacat
    20221 gcaaggcgtt aaatttttgc cgctctgggc gaattgtacg caaggggtgc ttatgcggtc
    20281 cgtaggacag aacaccacgg gtttacccgt ggatgaatga gcccg
//
LOCUS       DAOSWR010000017        19492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1767_length_19492_cov_5.257190, whole genome shotgun sequence.
ACCESSION   DAOSWR010000017 DAOSWR010000000
VERSION     DAOSWR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19492
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1767_length_19492_cov_5.257190"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PK513_02375"
     CDS             complement(<1..1219)
                     /locus_tag="PK513_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81331.1"
                     /translation="MPCAQEINETGAQHLQTILQNILDTRERQRSEQDKSTFEIDGTI
                     AVEAAETYYAITWPQMKLHSVNGDTIKIGLIAMNAVPHDKPGQYKITTAIPTPIIGQD
                     KDGQEILRITIGMQKAAGIFDESLNNFIKINTQLNDIQITFDGGKTLATIPKLQLSYN
                     FSEDDNARWSGPSYFEISDLKLTRPSESAALSIEKFIVTSHLDSFAAQALNGGIQHGK
                     QAPLSPLQFSDGAEITLAISGLSASSPNSPEKNNKKNESFSLQSGAFNFRYENALSGS
                     AKAHTSFNFQNLITNDIPQDLQMLLPRTGSFSLTQHNIPLAEIDETLQNSAEASGKIL
                     SLPLLFKIPSILAQAGSYLEIRETFFENPEYRIDFDSILRADLAAVNSATAIGTLRFA
                     GLDKILSRAQVIGT"
     gene            1425..2588
                     /locus_tag="PK513_02380"
     CDS             1425..2588
                     /locus_tag="PK513_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81332.1"
                     /translation="MLNAQDTQYTLKEDAQIHYQPQVSNPLPGEAIAMLVKGERILKP
                     KVVMLDGASIGDVDRVALRAHLEGWLERHIANVLEPLVALEDMGDAKAPVKGIAFQVY
                     QALGIAAREQLEDLIGELDADDRQDLRAKKIRLGPILVFIPALNKPAAVRLRALLWGL
                     WNDKDLPMDCPKDGIVSFAVDSKAVDKAFYQAIAYPVYGGRAIRIDMLDRVISGVYDA
                     AEGGKFKAAHQWAEWLGCSIEGLYEVLEAMGHQKVYDPAEAVEEAGREAQSEVAVKSL
                     EEEVVAEADAGNAAKDEKALQEKPEQEKAEQVKPELATFRLKKGKAFSKNKPVFKQSQ
                     GKQGKNFKDKPKGKRSKTANRSPKIMSTGPEKRLEDSPFAVLEQLKQKSNEKK"
     gene            2617..2952
                     /locus_tag="PK513_02385"
     CDS             2617..2952
                     /locus_tag="PK513_02385"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HOO81333.1"
                     /translation="MHFPSVQAARFSGDYLLQVCGSDEQGKELLPGGHIACQAYISGV
                     MDYHNLIRSLGAAPSVDFCVPEGTPLKEIHQKVWGYIASHHHEQGPFIAAPGVALGLF
                     SAYPCEASD"
     gene            complement(2963..4006)
                     /locus_tag="PK513_02390"
     CDS             complement(2963..4006)
                     /locus_tag="PK513_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO81334.1"
                     /translation="MNDCCHTSTQETSTKKRFDTIMFGALTIIIVTLILNAFDLNIPG
                     LATFAKTQIELLMAMSWGVALGLLSVGLMNKIPRTYFTAILGNGDKFTDILKAAIAGV
                     ILDLCNHGILVISGKLYERGLSIAQVLTFLIASPWNSLSLTFILIALIGLKWTLVFTL
                     ASVVIAVISGMIYLRLVKAGKLPTNPHSEEIPEDFDIRADMKARLKGWRPSLKWAGEI
                     VHDGWKDGQMIIRWILFGAVVAAAMRAFIPAEMFTHWFGPSLLGLGITMIAATIIEVC
                     SEGSAPIATEIMNTGGAPGNGFAFLMAGVSTDYTEILIVREFTKSWKIALSLPLITVP
                     QIIVLGLIFNTIH"
     gene            complement(4010..4285)
                     /locus_tag="PK513_02395"
     CDS             complement(4010..4285)
                     /locus_tag="PK513_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOO81335.1"
                     /translation="MSDKHPCHKAEIPKLNRITGQVEGIKRMIEDRRYCTDILAQLRA
                     ARAAIRTVEAHVLETHLHHCVTEAMTAENAQKKIDEIKDLFKRYDEV"
     gene            4386..4799
                     /locus_tag="PK513_02400"
     CDS             4386..4799
                     /locus_tag="PK513_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81336.1"
                     /translation="MVRLPERIVYVDDEQDLREILRVTLESFGYEGALATCGSGKELL
                     QRLRVLQPELILLDLKMPDMDGPATIAQLKMHEMGRNIPIVFVTGSIKLEMLERYKPL
                     GVIGVIHKPLDLGALTDNISALWCEYLGEEAVVAD"
     gene            complement(4796..5803)
                     /locus_tag="PK513_02405"
     CDS             complement(4796..5803)
                     /locus_tag="PK513_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO81337.1"
                     /translation="MTKTATQKADASPLDELSALLDSDMRAVNAMILARMDSPVKLIP
                     QLASYLIAAGGKRVRPLLTLASTAMYEGEMTRAHRLAASVEFIHTATLLHDDVVDESR
                     ERRGQDAANLIFGNQASVLVGDFLFSRAFQLMTEDGSLDILRILSDTSAIIAQGEVMQ
                     LTTANNLKTTMNDYIEVIKAKTAALFAAACEVGPVIAGANTAAARAMAAYGMNIGIAF
                     QIADDALDYMAAREDLGKTIGDDFREGKMTAPVLFALQNANDEERAFWTRTMSNREIN
                     DDDFDHARTLIEQGNIIQQCFDLAYTYRDKAIEALKQAPACEIRDLLESLTDYTINRK
                     S"
     gene            complement(5845..6372)
                     /locus_tag="PK513_02410"
     CDS             complement(5845..6372)
                     /locus_tag="PK513_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81338.1"
                     /translation="MNRLITTIFCLSLLIAFPAQAQQREYKSGLNYTTLGSKPKPAPA
                     PKPQVHPAENAIESEETPVTELEDKAKESPDPATRIWNKYKELATGTAGEKEQAAKTE
                     NNKPEAPEKPDVPLKPKTKLTKQSAQDPKPNNGFGAILENWKNTKDTRRDMRSMSFKT
                     PDLPTKPSVEKPQTP"
     gene            6410..6925
                     /locus_tag="PK513_02415"
     CDS             6410..6925
                     /locus_tag="PK513_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81339.1"
                     /translation="MENLRIVRAGVDDLSRLHDMAMAMKAAKDVGYFETAMEYQDAGE
                     RLVFIAEYEGQMAGYCMLAWNPKYAFYRAMGYPEIQDLNVLPDFRQRGIASAMIEKCE
                     GLAREKGHSHMGISVGLTASYGSAQRLYFKLGYVPDGHGVTYDRQPVAFGEFRPVDND
                     LCLMLVKDLQS"
     gene            7001..9727
                     /gene="ppdK"
                     /locus_tag="PK513_02420"
     CDS             7001..9727
                     /gene="ppdK"
                     /locus_tag="PK513_02420"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOO81340.1"
                     /translation="MTKWVYSFGKEHNEGDATMCNLLGGKGANLAEMAGLGLPVPPGF
                     TITTEVCTAYYENEEQYPAELKAQLEEGVERIEAQMGAKFGNAENPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDETVKGLEARSGDARFAWDSYRRFIQMYGDVVLGVDHHEFEDVI
                     ARYKRDEDLVQDTDISAEGWQEIVDEYKTVVERETGKPFPSDPHEQLWGAVGAVFGSW
                     MAPRAITYRKINEIPGDWGTAVNVQSMVFGNMGDDCATGVAFTRDPATGENYFYGEYL
                     INAQGEDVVAGIRTPQSLTKKGREKECSDLPSMEETMPEVFKQLDDVRIKLETHYKDM
                     QDLEFTVQKDQLFMLQTRSGKRTAKAALKVAVDMVEEGLITQDEALLRVDPQSLDQLL
                     HPTLDPKAEKDVLTRGLPASPGAACGKVVFTSEEAEVRAHGEENVILCRQETSPEDIA
                     GMHAARGILTSRGGMTSHAAVVARGMGTCCVAGAGELHIDVQGKVIRVGEREIREGET
                     LTIDGSSGEVMFGEVATIQPELSGDFGMLMEWADKRRRMKVRTNAETPLDAKTAREFG
                     AEGIGLCRTEHMFFDAERIQNVREMIFANDEEERRAALDKLLPAQRGDFASLFEIMNG
                     LPVTVRLLDPPLHEFLPHAQDDIDQFAKTAGISQADVRQRLNELHEFNPMLGHRGCRL
                     GITYPEIYEMQARAIFEGALEAADKTGKPPVPEIMIPLVGAVRELEILRGKVEAVAAQ
                     VFEEKGESLDYMIGTMIELPRAALTADEIAQAADFFSFGTNDLTQTCLGMSRDDAANF
                     LPAYVGQGIYERDPFASLDVDGVGRLIEVAIHGGRQTNATIKLGICGEHGGDPASIAF
                     CERVGLDYVSCSPYRIPIARLAAAQASLRVQEEGRLGKSEASTKANGAQKAA"
     gene            9827..10045
                     /locus_tag="PK513_02425"
     CDS             9827..10045
                     /locus_tag="PK513_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81341.1"
                     /translation="MSENTDKKEKDGGCGGHCGCKHGEKGEQKNGEVLKTFDMYHFFS
                     RKDVIFVMAFVIAFLIFTLAVKLAPEII"
     gene            complement(10042..10758)
                     /locus_tag="PK513_02430"
     CDS             complement(10042..10758)
                     /locus_tag="PK513_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOO81342.1"
                     /translation="METNMPEKPFFVTLKNRGLIYLDGPDRYAFLQGLITNDIEKLTP
                     EKSLYACLLSPQGKFLHDFFVIESVEETTLIDCEGGARARDLFERLNTYKLRKSVTLS
                     LDEHKDVYAVFGRNYGWPDPRHSKMGDRCFFIDQRDQKNMPEKPFEEWDRCRIELTIP
                     DGSRDLIPEKSTMDEGNMDRFNAIDYQKGCYVGQELTARMHYRGLGKKRLQTVSLSDL
                     PNGAELRSTCGDIGIALVKS"
     gene            10757..11722
                     /locus_tag="PK513_02435"
     CDS             10757..11722
                     /locus_tag="PK513_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOO81343.1"
                     /translation="MKILFITSSRIGDAVLSTGLLRHIEQRYPDARVTVVCGPLAASL
                     FEGYPLLERLVSFKKEKHNMHWVKLWRQVVEMRWDMVVDLRNSAVSRLIMAKERYIFG
                     GCAVKAGHKVEQNAAVMKLGHQVPGPKLWFTDVQKEKAAALIPEGGPVLGVGPTANWI
                     GKTWSVERFVEVVRFMTGSGGVMEGARVAVFGAPGEEDDALKVLESIPEERRIDCIAK
                     GTPGDAAAAIARCDFYLGNDSGLMHAAAAALVPTLGLFGPSYDLIYRPWGGYADFVRT
                     PESFDQLIDFGGYNSKTLNHTLMGSLSVDAVKDKITEMFEKRKAA"
     gene            complement(11719..12255)
                     /locus_tag="PK513_02440"
     CDS             complement(11719..12255)
                     /locus_tag="PK513_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81344.1"
                     /translation="MFKKHHALVFGLVSVVAITACTPGKEIGDIGGQFWQRANVSDSI
                     YMQGPKAQQMLNRDISRCVIDLRELERLGTLKDAIPTDFRGRTLNPDEQELYDWDTPE
                     RNKHLYAEHSNYTNFESCMEERGWERVEHVPFDVSRKGRENYLRAHVDYHEKSDEAKT
                     KSRHQTSNDIGDYSDLND"
     gene            complement(12371..14089)
                     /locus_tag="PK513_02445"
     CDS             complement(12371..14089)
                     /locus_tag="PK513_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO81345.1"
                     /translation="MLFGLAMHGQPKYDANAAHLDYANPDAPKGGKLKIAAIGTFDTL
                     NPFSIKGKAAQGLNLVYDRLMARVWDEPFTTYPLIARSVEIPEDRSSITVHINPKARF
                     HDGSPITADDVIFSFETLKASGRPNMRQIYRLVDQVIRRDPLTVYFHFGEGYDRETAM
                     IIARMPVLSKAYWEGRTFDQTTLDIPVLNGPYRIKDFDAGRRITYERVPDYWAKDLLT
                     NVGHHNFDEITYEYYRDDTVAFEAFKTGDLSLRREWDAGKWASAYDFPAAKDGHVKLE
                     ALPHGRPERARALIFNTRRTPFDDIRVRKALNLLFDFEWINKNIFHGQYKRSASFFPN
                     SELAATGTPQGMELDILNLWRNDLPPEIFDSLFDFLHPTDIKKQSPREKMRQADQMLK
                     DAGWIVENGKRVKDGQPFSFEIIVDAPENEKIALHFKRALEKMGIEVLIRVLDAAAYR
                     GRLNEYDFDMTIYYWLSSLSPGTEQNLYWTCKAANEPARWNFPGICSPTIDALAGSIA
                     SSETREELVARMRALDRILVHGHYIIPLYYSGVDYVGYWPPITSPETTPLYGMVIETW
                     WMPGKN"
     gene            14166..14720
                     /locus_tag="PK513_02450"
     CDS             14166..14720
                     /locus_tag="PK513_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOO81346.1"
                     /translation="MGLFIAEAVRASNSYLDRIELVQGNIAAQETDAVVTLLPQTLEY
                     RGSVNKAIQRAAGHRMDEFLYRNVVNPQAGDVYAVPGFKMGCRNVFFAIVPVWKDEWD
                     RHDRQLLNAARKTMELARRMSLRSIAFPPIGAGQKGFPKERAARLIIQGIADRLDADF
                     DEVRIVCQCSETMQIFEKRLRRSF"
     gene            14842..15399
                     /locus_tag="PK513_02455"
     CDS             14842..15399
                     /locus_tag="PK513_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81347.1"
                     /translation="MEKWKKKFAGQAFAAATLTGVSLWSGFATKSFLDERDVLRSGPE
                     WDGLVAEFKPLYREYCERSLLSSDLRAALENQREQIVQRAVDSSSPVSLRDYVFLSGN
                     VPFPSEERIGKCQSEIAMAAAQDAVYEGGRNKLAFTSLAVLSGVLSAGFALVSARSAV
                     RTYRRREEDEPVQGPSASAPTSSHS"
     gene            complement(15396..16397)
                     /locus_tag="PK513_02460"
     CDS             complement(15396..16397)
                     /locus_tag="PK513_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOO81348.1"
                     /translation="MSQPQFDVVAVGNALVDVLSQTDEAFVQDQNARYGMEKGAMTLI
                     DEARAVELYAQMPAGIESSGGSAGNTMAGFASFGGQGGFIGKVADDDLGRIFQHDLRS
                     LGIQFDTQPLIMGAKTGRCLICVTPDAQRTMNTFLGASVELTPGDIDEDLIAKAKITY
                     LEGYLFDRDTAKQAFIYAAETAHAAGHRVALTLSDPFCVDRHRSDFLRLVESHIDILF
                     ANEEEIKSLFMQDNFEDAASAVSKHVEIAAMTRSEKGAVIRNGEKTYEISAAPVSEVI
                     DTTGAGDQFAAGFLYGFTSGKDIETCGKLGALAAAEVIGHMGPRPEHSYKELIAKAA"
     gene            16484..17062
                     /locus_tag="PK513_02465"
     CDS             16484..17062
                     /locus_tag="PK513_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOO81349.1"
                     /translation="MAKKTEKDLKSTIVLKALEQAEQMGWANVALSDVAEAAGVSLAE
                     LFDLVEDKADILALFGRMIDRRVLEAVSKSDPSVSERDRLFDIMMERYEALNDYRAGL
                     VSILESFRFDPKQALISAPHLCRSMSWMLEAAGLETSGVRGALKVAGLTGVYLKTLRV
                     WRDDESADLSRVMAALDKDLGRAEQFANTLGF"
     gene            complement(17059..17940)
                     /locus_tag="PK513_02470"
     CDS             complement(17059..17940)
                     /locus_tag="PK513_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO81350.1"
                     /translation="MIFVFGAILQSIRITTDPEETFGAVHYAKHYKNSIGGKGALQAL
                     AAAKAGAKTALAGKMGDDELAKHILLRLRTHGVITSGVAKAEKLQTGTSIRIVDENRT
                     ILAMGASAYTKAHQLPMETLNSDSTVVLQTEINMEENSKLLATAKKAEATTILNLAPK
                     AYISDEDLANLDYLIAPSASKKALNHILKAKHLTTIFISTDGSCELIVEGGPPSKVPK
                     PAHEALNWKYPEGAEDTFCGTFAAGIDMGLSLEKAIERAHIAASLTASVNGAYDAIPF
                     SDMVDECIKALQAQKES"
     gene            18116..19348
                     /gene="hemA"
                     /locus_tag="PK513_02475"
     CDS             18116..19348
                     /gene="hemA"
                     /locus_tag="PK513_02475"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HOO81351.1"
                     /translation="MTDRFDYHTHFEGCVDQVKQEGRYRYFANLERKVGDFPRAIYRM
                     PNGQSKEVTIWCNNDYLGMGQHPKILAAARAALENSGSGAGGTRNISGTTCYVVELEE
                     SVADLHSKEAGLVMSSGYVANEGALGTLGKLMPECAFFSDELNHASMIHGIKDSKQQK
                     FIYRHNDMGHLESLLKSVDLDRPKIIAFESVYSMEGDISPMEEICDLADKYNAFTYLD
                     EVHAVGVYGPRGGGVSEERGLMDRIDVIEGTFGKAYGAMGGFITGDAVIIDAVRSYAP
                     SFIFTTALPPAVSAAAMAAVEHLKNSDVERQRVRRNVKLLKDGLTQAGLKFLPGDSHI
                     VPLIVGEPNCCRELTDILMNDHDIYVQPINYPTVPRGTERLRLTATAAHSEEDVLHMV
                     STLRHLWETNHQLMQIAA"
BASE COUNT         4338 a         3917 c         5872 g         5365 t
ORIGIN      
        1 ttgtgccgat gacttgtgcg cgggagagga ttttatccag tccggcaaaa cgcaatgttc
       61 ctatggctgt ggcgctgttt acggctgcga gatctgcgcg cagaatgctg tcaaaatcga
      121 tgcgatattc cgggttttcg aagaaggttt cgcggatttc cagatagctt ccggcctggg
      181 cgaggatgga agggattttg aaaaggagcg gcaggcttaa aatttttccg gaggcttcgg
      241 cgctgttttg cagcgtttcg tcaatttccg ccagtgggat gttatgttgc gtcaggctaa
      301 aacttccggt gcggggaagc agcatttgta agtcttgcgg gatgtcgttc gtaatcaagt
      361 tctggaagtt gaatgatgta tgggctttgg cagatccgct taaggcgttt tcgtatctga
      421 aattaaaagc gcctgattgc aggctaaagc tttcgttttt cttattgttt ttttcggggc
      481 tatttgggct ggatgcactc aagcctgata tagcgagcgt tatctctgcg ccatcggaga
      541 attgcaacgg cgaaagcggg gcttgtttgc cgtgctgtat gccaccgttt aaggcttgtg
      601 ctgcaaagct gtcaagatgg ctagtgacaa tgaatttttc aatggaaagt gctgcgcttt
      661 cactggggcg ggtgagcttg aggtctgaaa tttcgaaata tgatgggcca gaccagcggg
      721 cattatcatc ttcgctgaaa ttatagctga gttgtagttt tggaattgtt gctaaagttt
      781 tgccaccatc gaatgtaatt tgtatatcat tgagttgtgt atttattttg atgaaattat
      841 ttaaggattc atcaaaaata ccggctgctt tttgcatgcc gatggtaata cgcagaattt
      901 cctgtccgtc tttgtcctgt ccgataatcg gtgtggggat ggcggtggtg attttatatt
      961 ggccgggttt atcatgcggc acggcgttca tggcgatgag gcctattttg attgtgtctc
     1021 cattcacgct gtggagcttc atttgcggcc aggtaatggc gtaataggtt tcggcggctt
     1081 cgacggctat ggtgccgtcg atttcgaatg tacttttatc ttgttcgctg cgttgccgct
     1141 cgcgcgtatc cagaatattt tggagtatgg tttggaggtg ctgtgcgcct gtttcattta
     1201 tttcctgtgc gcagggcatt gttggccatg cgctgaggat tacaaatgcc aggataagtg
     1261 tttgcgttat ttttagcacc agtgtcactt tcaaaattgg ttggaaagtt ttcccgggta
     1321 tttaaacaaa aaatctataa ggatgctatg aatgttttaa ttggacatcc gcgcggcttt
     1381 acgtttatat cgaagcctta ttgatatggg gtttgcaagg aacaatgtta aacgcgcaag
     1441 acacacaata tacgctgaag gaagacgctc agattcatta tcagccacaa gtcagcaacc
     1501 cgctgcctgg cgaggcaatc gcgatgctgg ttaaaggcga gcgtattttg aagcccaagg
     1561 tcgtaatgct ggacggggct tcaattggcg atgttgatag agtggctttg cgtgcacatc
     1621 tggaagggtg gcttgagcgg catattgcca atgttctgga gccgttggtg gcgcttgaag
     1681 atatgggtga tgccaaggct ccggtgaaag gtattgcgtt tcaggtatat caggcgctgg
     1741 ggattgcggc gcgtgaacag cttgaggatt tgattggtga actggatgcg gatgatcggc
     1801 aggatttgcg ggcgaagaaa attcggctgg ggccgatttt ggtgtttatt ccggctctga
     1861 acaagcctgc ggcggtgcgg ttgcgggcac tgctttgggg gctgtggaac gataaagatt
     1921 tgccgatgga ttgtccgaaa gacggaattg tatcttttgc ggttgattca aaggcggttg
     1981 ataaagcgtt ttatcaggcg attgcctatc cggtttatgg cgggcgcgca attcgaattg
     2041 atatgttgga ccgggtgatt agcggagttt atgacgcggc ggaaggcggc aagtttaagg
     2101 ctgcgcatca atgggctgaa tggctgggct gttctatcga agggctttat gaagttctgg
     2161 aagcgatggg gcatcagaag gtttatgatc cggctgaggc agtagaagag gctggacgcg
     2221 aagcgcaatc tgaggtcgcg gtgaagtctc tggaagaaga agttgtggct gaggctgatg
     2281 ctgggaacgc agcaaaagat gagaaggctc tacaagaaaa gccggagcag gaaaaggctg
     2341 agcaggttaa gccggagctg gcaacgtttc ggttgaagaa ggggaaagcg ttttctaaaa
     2401 ataaacctgt gtttaaacaa tcacaaggaa agcaaggtaa gaatttcaag gataagccca
     2461 aaggcaagcg gagcaagaca gctaatcgta gtcctaaaat tatgagtaca ggacctgaga
     2521 aacgccttga agattcaccg tttgccgtgc ttgaacaact caagcaaaag tcgaatgaaa
     2581 aaaagtagag ctcttttatt ttttcttgtt tttttgatgc attttccctc tgtgcaggct
     2641 gcgcggtttt ctggcgacta tcttttgcag gtttgtggat cagacgagca gggcaaggaa
     2701 ttgttgcccg gggggcatat tgcgtgtcag gcttatattt ccggggtaat ggactatcat
     2761 aatttgatcc gttcgttagg tgccgcgccg agtgtggatt tttgtgtgcc ggaagggact
     2821 cctttaaaag aaattcatca aaaagtatgg ggctatattg caagccatca tcatgagcaa
     2881 ggaccgttta ttgcggctcc cggcgtagcg ttggggttgt tttccgctta tccgtgtgag
     2941 gcaagtgatt aggggctgac ccctagtgta tagtgttaaa aatcaggccc agcacgatga
     3001 tttgtggtac ggtaatcagc ggcagggaca gggcgatttt ccaggatttt gtaaactcgc
     3061 gtacgattaa tatttctgtg tagtcggttg aaacccctgc catcaggaag gcaaagccgt
     3121 ttccgggggc accgcctgtg ttcataattt cagtggcaat gggtgcggag ccttcggaac
     3181 aaacctcaat aatagtggcg gcgatcatgg tgataccaag gcccagcagg gacgggccaa
     3241 accagtgggt gaacatttcg gccgggatga atgcgcgcat ggcggcggcg acaactgcgc
     3301 cgaagagaat ccagcggatg atcatctgtc cgtctttcca gccatcgtgc acgatttctc
     3361 cggcccattt caatgacggg cgccagcctt tgagccgggc tttcatatcg gcgcggatgt
     3421 caaaatcttc tggaatttct tcactgtgtg ggttggtagg cagtttaccg gctttgacta
     3481 gtcttaagta gatcataccg gaaataaccg cgattaccac ggaggcgaga gtgaaaacca
     3541 gcgtccattt taaaccgata agcgcgatga gaatgaatgt aaggctgagc gagttccacg
     3601 ggctggcgat gagaaaggtc aggacctggg cgatggacag gccgcgttcg tataattttc
     3661 ctgatatgac aagaatgcca tgattgcata gatcaaggat tacgcccgca atggcggctt
     3721 tgagtatgtc tgtgaattta tctccattgc cgaggatggc agtgaaatag gtgcgcggga
     3781 ttttattcat cagcccaaca ctgagcaggc caagcgcgac tccccatgac atggccataa
     3841 gaagttcgat ttgggttttg gcaaaggttg ccaggccggg gatatttaag tcaaacgcat
     3901 ttaatatcag tgtaacaatt atgattgtaa gcgcgccgaa catgatagtg tcgaagcgtt
     3961 ttttagtgct ggtttcctga gtagaggtat ggcagcaatc gttcatgggt taaacttcat
     4021 catagcgttt gaacaggtct ttgatttcgt caattttttt ttgtgcgttt tcggctgtca
     4081 tggcttcggt tacgcaatga tgcaggtggg tttcgagtac atgcgcttca accgtccgga
     4141 tggccgcgcg tgcggcacgg agttgggcga gaatgtctgt gcaatagcgc ctgtcttcaa
     4201 tcatgcgttt tatgccctca acttgtccgg tgatacggtt taatttcggg atttcagctt
     4261 tgtggcaggg gtgtttgtcg ctcatgtctg tcaccatata cccccatagg gtatatggca
     4321 agagctttca aggtcttgcg gatattcgcg tatgtatact atacttaaga tgcaggggga
     4381 tttttatggt tagacttccg gaacgtattg tttatgttga tgatgagcag gatttacggg
     4441 aaattctgcg ggttactctt gagagctttg gttatgaagg tgcattggca acgtgcgggt
     4501 ctggaaaaga gttgttgcaa cgtttacggg tattgcagcc agagttgatt ttgctggatt
     4561 taaaaatgcc ggatatggat ggtcctgcga caattgctca gttgaaaatg catgaaatgg
     4621 gcaggaatat tccgattgtc tttgtgacgg gcagtataaa actggagatg cttgagcgct
     4681 ataaacctct tggcgtcatc ggggtgattc acaagccgtt ggatttgggt gcattgacag
     4741 acaatatcag tgcgctgtgg tgtgagtatc ttggtgaaga ggctgtggtt gcagattaag
     4801 atttacgatt gatcgtgtaa tcggtcaggg attcgagcag gtcgcgaatt tcgcatgcgg
     4861 gcgcttgttt taaagcttca atcgctttgt cacggtacgt ataggctaaa tcaaaacatt
     4921 gttggataat gttgccttgt tcaatcaagg tacgggcgtg gtcaaaatca tcgtcgttga
     4981 tttcgcggtt gctcatggtg cgggtccaga atgcacgctc ttcatcgttg gcattttgta
     5041 aggcgaagag tacgggggcg gtcattttgc cttcgcggaa gtcatcgccg atggttttgc
     5101 cgagatcttc acgtgcggcc atataatcga gcgcgtcgtc ggcgatttga aaagcgatac
     5161 cgatattcat gccataggcg gccatggctc tggcggctgc ggtattcgcc ccggcgatca
     5221 cgggtccgac ttcgcaggcg gcggcgaaga gggcggcggt tttggcttta atgacttcaa
     5281 tataatcatt cattgtggtt ttgagattgt tggcggtggt gagctgcata acttcgcctt
     5341 gagcgatgat ggccgaagta tcggagagaa tgcgcaatat gtcgagcgaa ccgtcttcgg
     5401 tcatcagttg aaaggcgcgg gaaaagagga agtcgccgac cagtacgctg gcctgattgc
     5461 cgaagatgag attggcagcg tcttgtccac ggcgctcccg gctttcatca acgacatcat
     5521 cgtggagcag ggtagcggta tggatgaact cgacgctggc ggcgaggcgg tgggcgcggg
     5581 tcatttcacc ttcatacatg gctgtgctgg ccagtgtcag gagcgggcgg acccgtttac
     5641 cgccagcagc gatgaggtat gaggcgagct gcgggataag ttttacgggg ctgtccatgc
     5701 gcgccaaaat catcgcgtta accgcacgca tatcagaatc gagcagggcg ctcaattcat
     5761 caagcgggct ggcgtcagcc ttttgggtag cggttttggt catacatagt tttaaatcag
     5821 ttttttgcgt gtgaggcaag accattaggg tgtttgcggt ttttcgacag acggtttggt
     5881 aggcaggtcg ggagttttaa agctcatgga gcgcatgtcg cggcgggtgt ctttggtatt
     5941 tttccagttt tcaaggattg caccgaaccc attatttggc tttgggtctt gtgcgctttg
     6001 ttttgtgagt tttgtttttg gcttcagcgg tacatcaggt ttttctggtg cttccggttt
     6061 gttattttct gttttggcgg cttgttcttt ttcaccagcg gtgcctgtgg caagttcttt
     6121 atatttgttc cagatgcgcg tggcgggatc gggggattct tttgctttat cctctaactc
     6181 tgtcaccggt gtttcttcac tttctatggc gttttcggct gggtggactt gtggttttgg
     6241 cgcaggcgca ggtttgggtt tgctacccag tgtggtgtag tttaggcctg atttatactc
     6301 gcgctgctgg gcctgtgcag gaaaggcgat gaggaggctt aaacaaaaaa tggttgtgat
     6361 gaggcgattc atgggcctta tattacgaaa gggagccttt gggatgcaaa tggaaaatct
     6421 aagaattgtg cgagcaggcg tggatgatct ttcgcggttg cacgatatgg cgatggcaat
     6481 gaaggcggcc aaggatgtcg ggtattttga aacggcaatg gagtatcagg atgcgggtga
     6541 acggttggtt tttattgccg agtatgaagg gcagatggcc gggtattgca tgctggcatg
     6601 gaatccgaaa tatgcgtttt accgggcgat ggggtatccg gaaattcagg atttgaacgt
     6661 gctgcctgat tttcgccagc gcgggatagc tagtgcgatg attgaaaaat gtgagggttt
     6721 ggcgcgggaa aaaggccact ctcatatggg aatcagtgtc ggactgacgg cgtcttatgg
     6781 ttcggcgcag cggctgtatt ttaagctggg ttatgtccct gacgggcatg gtgtaacgta
     6841 tgatcgccag ccggtcgcat tcggggaatt ccggcctgtg gataacgatc tgtgtttgat
     6901 gctggtgaaa gatttgcaaa gttaaaagaa tatgctaaac cctgcgtgaa tgatatgagt
     6961 tctgggcgtt ttaaggtcgt cttcaaggag aatgcagagt atgactaaat gggtatatag
     7021 cttcggtaaa gagcataatg aaggtgatgc cacgatgtgt aatcttttgg gtggaaaagg
     7081 ggcgaatttg gcggagatgg ccggtttggg gcttcctgtg ccgccgggtt ttacgattac
     7141 caccgaagtt tgcacggctt attacgagaa tgaggaacaa tatccggcgg agcttaaagc
     7201 acaacttgaa gagggggtgg agcggattga ggcgcagatg ggcgcgaagt tcgggaatgc
     7261 cgagaacccg cttttggtgt ctgtgcgctc tggtgcgcgc gtatctatgc cggggatgat
     7321 ggatacggtg ctcaatttgg ggctgaatga cgagacggtt aaaggccttg aggcgcgctc
     7381 tggtgatgcg cgttttgcgt gggattcgta tcgccggttt atccagatgt atggcgatgt
     7441 ggttttggga gtcgatcatc atgaatttga agatgtgatt gcacgttata agcgtgatga
     7501 ggatctggtt caggataccg atatctctgc tgaaggttgg caggagattg tcgatgaata
     7561 taagacggtg gttgagcgtg aaaccggcaa gccttttccg agtgatccgc atgagcagct
     7621 ttggggcgct gtgggcgctg tgtttggttc atggatggcg ccgcgcgcga ttacctatcg
     7681 taagatcaat gaaattccag gagattgggg cacggctgtt aatgtgcaat ccatggtgtt
     7741 tgggaatatg ggcgatgatt gtgcgaccgg cgtggctttt acacgcgatc cggctacggg
     7801 tgagaattac ttttatggcg aatatctgat taatgcacag ggcgaagatg ttgtggcggg
     7861 gattcggacg ccgcaatctt tgaccaagaa gggccgtgag aaagaatgca gtgatctgcc
     7921 gtcgatggaa gagactatgc ctgaggtttt caagcagctt gatgatgtgc ggatcaagct
     7981 tgagacacat tataaagata tgcaggacct tgagtttacg gtgcaaaagg accagctttt
     8041 catgctgcaa acccgtagtg gcaagcgtac agcgaaagcg gcactcaagg ttgctgtgga
     8101 tatggttgaa gaaggtttaa ttacacaaga tgaggcgctt ttgcgcgttg atccgcaaag
     8161 tctggatcag ctgcttcatc ccactttgga tccaaaagcc gaaaaggatg ttttgacgcg
     8221 aggccttccg gcttcacccg gggcggcgtg tgggaaggtt gtgtttacct ccgaggaagc
     8281 ggaagtcaga gctcatggcg aggaaaatgt gattttgtgc cgtcaggaaa cttcgcccga
     8341 agatattgcc gggatgcatg cggcgcgcgg gattttaacc tcacgcggtg gaatgacctc
     8401 tcatgcggct gtggtggcgc ggggcatggg gacatgctgc gtggctggag ccggtgagct
     8461 tcatatcgat gtacagggca aggtgatccg tgttggtgaa cgtgaaatca gagaaggtga
     8521 gacacttaca attgacggat cttccggtga ggtaatgttt ggcgaggttg cgaccattca
     8581 gcctgagttg tctggtgatt ttgggatgct gatggaatgg gcggataagc gccgccggat
     8641 gaaagtgcgc acgaacgcgg aaacgccttt ggatgccaag accgcgcggg aattcggagc
     8701 ggaagggatt gggttgtgcc ggaccgagca tatgtttttt gatgctgagc gtattcagaa
     8761 tgtgcgcgag atgatttttg cgaatgatga ggaggaacgc cgggcggctt tggataaatt
     8821 gcttccggcg cagcgcgggg attttgcctc gttgttcgaa atcatgaacg gtttgcctgt
     8881 gacggtgcgt ttacttgatc cgccgttgca tgaatttttg ccgcatgcgc aggatgatat
     8941 tgaccagttt gccaagacgg cagggatttc ccaggcggat gtgcgccagc gtttgaatga
     9001 gctgcatgaa ttcaatccga tgctggggca ccggggttgc cgcctcggga ttacttatcc
     9061 ggaaatttac gagatgcagg cacgggcgat ttttgaaggt gcgctggagg ctgcggacaa
     9121 gactggcaag ccgcctgtac cggagattat gattcctctg gttggtgcgg tccgtgagct
     9181 ggagatttta cgtggcaagg ttgaagcggt ggcggcgcag gtatttgaag aaaaaggtga
     9241 gagtctggat tacatgatcg ggacgatgat tgagctgccg cgcgcggcgc tgacggcgga
     9301 tgaaattgcg caggcggctg atttctttag ttttggcacg aatgatttga cgcaaacgtg
     9361 tttgggaatg agccgggatg atgcggcgaa cttccttccc gcctatgtcg ggcaagggat
     9421 ttatgaacgc gatccgtttg catctttgga tgttgacggg gtcgggcgtt tgatcgaagt
     9481 ggcgatacat ggcgggcggc agacgaatgc gacaatcaag ctggggattt gcggcgaaca
     9541 tggcggcgat ccggcctcga ttgcattttg tgaacgcgtc ggtcttgatt atgtgtcctg
     9601 ttcaccttac cgcataccga ttgcacggtt ggcggcagcg caggcatccc ttcgggtgca
     9661 ggaagagggg cgtctgggaa agtctgaggc ctcgactaag gcaaacgggg cgcaaaaggc
     9721 ggcttaaatt tataatcgtc attgcgagcg atagcgaagc aatctagaat atggattact
     9781 tcctcggtct tacggccttt ctcacagtga taaaagggat ggtgttatga gtgaaaatac
     9841 ggacaaaaaa gaaaaagatg gtggttgcgg cggccattgt ggctgtaagc atggcgaaaa
     9901 aggcgagcag aagaacgggg aagttctgaa aaccttcgat atgtatcatt tcttcagccg
     9961 taaagatgta attttcgtga tggcctttgt tattgcattt ttaattttta cgttggcggt
    10021 taagttggcg ccagagatta tttaggattt cactaaagct attccgatat caccgcaggt
    10081 ggagcgtagt tctgcgccgt tgggcaggtc gctgagcgag acggtttgca ggcgcttttt
    10141 accgaggccg cgatagtgca tgcgggcggt gagttcttgt ccgacataac aacccttttg
    10201 gtaatcaatc gcattgaagc ggtccatatt tccttcatcc attgttgatt tttcggggat
    10261 gagatcgcgg ctgccatcgg ggatggtcag ctcaatgcgg cagcggtccc attcttcaaa
    10321 tggtttttct ggcatatttt tttgatctct ttgatctata aaaaagcagc ggtctcccat
    10381 tttggagtgg cgaggatccg gccagccgta gtttcgaccg aataccgcat agacatcttt
    10441 atgttcatcg agtgagagtg tgaccgattt acgaagtttg tatgtgttta aacgttcaaa
    10501 aagatcgcgg gcgcgcgccc cgccttcaca gtcgatgaga gttgtctctt caacgctttc
    10561 aattacaaaa aaatcatgca gaaatttgcc ttgcgggcta agcaggcagg catagagtga
    10621 tttttcaggg gttagttttt caatatcgtt ggtgatgagg ccttggagga aggcgtaacg
    10681 gtctgggcca tccaagtaga taagaccgcg gtttttcagg gttacgaaga atggtttttc
    10741 tggcatattg gtttccatga agatcctttt tatcacatct tcgcgcattg gcgatgccgt
    10801 tttatcgacc ggattgttgc gccatatcga gcagcgctat ccagatgcgc gggtcactgt
    10861 tgtttgtggg ccgcttgcgg cttcgttatt tgagggctat ccgcttttag agcgcctggt
    10921 ttcctttaag aaagaaaagc ataatatgca ttgggtcaag ttgtggcgac aggttgtcga
    10981 gatgcgctgg gacatggttg tggatttgcg caattcggcg gtttcgcggc tgattatggc
    11041 caaagagcga tatatttttg gcggttgtgc agttaaggcc gggcataagg tggagcagaa
    11101 cgcagctgta atgaagctgg ggcaccaggt gccggggccg aaattgtggt ttacggatgt
    11161 gcagaaagaa aaggcggcgg cgcttattcc tgaaggtggg ccggtgttgg gcgttgggcc
    11221 gacggcgaac tggattggca agacctggtc ggttgaacgg tttgttgaag tggtgcggtt
    11281 tatgacgggt tcgggtggtg tgatggaagg tgcgcgtgtg gcggtgtttg gtgcgccggg
    11341 tgaggaagac gatgcgttga aagttctgga aagcattcct gaggagcggc ggattgactg
    11401 cattgccaaa ggaacgccgg gggatgcggc ggcggccatt gcacggtgtg atttttatct
    11461 gggcaatgat tccggtttga tgcatgcggc cgctgcggcg ctggtgccaa cactcgggct
    11521 gtttgggccg agctatgatt taatttaccg gccatggggc gggtatgctg attttgtacg
    11581 tacgccggag agctttgatc agttgattga ttttggaggg tataattcaa agactctgaa
    11641 ccatacgctg atggggtcac tgagcgtaga tgctgtgaaa gataagatta ccgagatgtt
    11701 tgaaaaacgc aaagcggctt aatcgttcag atcgctgtaa tcgccaatat cgttgcttgt
    11761 ttggtggcgg ctttttgttt ttgcttcgtc agatttttca tgataatcga cgtgggcgcg
    11821 caggtagttc tcgcggcctt tacggctgac atcaaagggg acatgttcaa cgcgttccca
    11881 tcctctttct tccatgcatg attcaaagtt cgtgtaattg gagtgttcag cgtagagatg
    11941 tttgttgcgt tcaggcgtat cccagtcgta aagctcttgc tcgtcagggt tgagggttcg
    12001 gcctcgaaag tctgttggaa ttgcgtcttt gagcgtgccg agacgttcaa gctctcgcaa
    12061 atcgataacg cagcgtgaaa tatcgcggtt tagcatttgt tgggctttgg ggccttgcat
    12121 gtagatcgag tcggaaacgt tggcgcgctg ccagaactgc ccgccaatat caccgatttc
    12181 cttgcctggt gtgcaggcag tgatggcgac tacagatact aaaccaaaaa ccagagcgtg
    12241 gtgttttttg aacattgaag tgcgttcctt aaagactcat aaacgattca aacctgggga
    12301 gatcatagca agggcgcatg ggcggctcaa tcatcattta cgaaagctta cggattgtcc
    12361 ccattactct ttaatttttt ccgggcatcc accatgtttc tatgaccatg ccgtaaagcg
    12421 gagtggtttc agggctggtg atggggggcc aataaccgac ataatcaacg ccgctataat
    12481 aaagcggaat gatgtaatgg ccatgaacca gaattcgatc cagtgcacgc atgcgggcca
    12541 ctagttcttc acgggtttcg gacgaagcga tgctgccggc cagcgcatcg atagttggac
    12601 tgcaaatgcc ggggaagttc cagcgcgccg gttcattcgc tgctttgcag gtccagtaaa
    12661 ggttttgttc cgtgccaggc gagagggatg agagccagta ataaatcgtc atatcgaaat
    12721 catattcatt cagtcgtccg cgataggcag ctgcatccaa tacgcgaatt agaacttcga
    12781 tgcccatttt ttccagagcg cgtttgaaat gcagggcgat tttttcattc tccggcgcat
    12841 cgacgatgat ctcaaaactg aatggctggc cgtctttgac gcgtttgccg ttttcgacaa
    12901 tccatcccgc gtctttgagc atttgatcgg cctgacgcat tttttcacgc gggctttgtt
    12961 tttttatgtc cgtgggatgc agaaaatcga aaagagaatc aaaaatttcc ggtggcagat
    13021 cgttccgcca gaggttcaga atgtccagct ccatgccttg tggtgttcct gtggccgcca
    13081 gttctgagtt tggaaaaaaa ctggcggagc gtttatattg cccgtgaaaa atattcttat
    13141 tgatccactc aaaatcaaag agcaggttca gggccttgcg cacgcggatg tcatcaaaag
    13201 gtgtgcgcct ggtgttgaaa atgagggcac gagcgcgttc cgggcgacca tggggcaggg
    13261 cttcgagttt tacgtgccca tcctttgccg ccgggaagtc gtaagcgctg gcccattttc
    13321 ctgcgtccca ttcgcggcgc agggagagat cgcctgtttt aaaagcttca aatgcgaccg
    13381 tgtcgtcgcg ataatattcg taagtgattt catcaaaatt atgatggccg acattagtaa
    13441 gcaagtcttt ggcccagtaa tcggggacgc gctcatatgt aatccggcgg cccgcatcaa
    13501 aatctttgat gcggtagggg ccgttgagaa cagggatatc aagtgtggtt tggtcgaagg
    13561 tccggccttc ccaatacgcc ttggacagga ccggcatcct ggcgataatc atggcggttt
    13621 cccggtcgta tccttctccg aaatgaaagt aaacggtgag aggatcgcgc cggatgacct
    13681 gatcgacgag acggtaaatc tggcgcatat ttgggcgccc ggaggctttc agcgtttcaa
    13741 aggaaaaaat tacgtcatct gcggtgatgg ggctgccgtc atgaaagcgg gctttggggt
    13801 taatgtggac agttatagag gagcggtctt ctggtatttc aacgcttcgg gcaatgagcg
    13861 ggtacgtggt gaagggttcg tcccagacgc gcgccatcag gcggtcataa acgagattaa
    13921 ggccttgagc ggccttacct ttaatggaaa aggggttgag cgtgtcgaat gtgccaatgg
    13981 ctgcaatttt gagctttccg cctttggggg catcggggtt ggcgtagtcc aggtgcgccg
    14041 cgttcgcgtc gtattttggc tgaccgtgca tggcaaggcc gaaaagcatg tttttttgcg
    14101 cccaagaagc cgaaaaagag caaaaaagta atagaacggt aatatatatc agtttattat
    14161 aatgcatggg gttattcata gcagaggcgg tgcgagcctc caattcttat ttagatcgta
    14221 tcgagcttgt gcagggaaat attgctgctc aggaaaccga tgccgttgtc actttacttc
    14281 cgcaaacgct cgagtatagg gggagcgtga ataaagcgat tcagcgtgct gccgggcacc
    14341 gcatggacga atttttgtat cggaatgtgg tcaatccgca agcgggggat gtttatgctg
    14401 ttcctggatt taaaatggga tgcaggaacg tgtttttcgc gattgtgcca gtttggaaag
    14461 atgagtggga ccgtcatgac aggcagttgc tgaatgcggc gcgcaagacg atggaactgg
    14521 cacggaggat gtctttacgc tctattgcat ttccgcctat cggggctggg caaaaaggct
    14581 tcccgaaaga gcgggcggcg cggttgatca tccaggggat tgccgaccgg ctggatgccg
    14641 attttgatga agtgcgcatt gtatgccagt gttcggaaac gatgcagatc tttgaaaagc
    14701 gtttgcgtcg ttctttttag gctgctttaa ccttgttatc aacgtttttt taagctgctt
    14761 cagccccaaa tgtatcattg acaagctgcg tacaaccaca taattttcta taaaaaaata
    14821 actgtgtttt gagatgttct tatggaaaaa tggaagaaaa aatttgctgg tcaagccttc
    14881 gctgcggcca cattaacagg cgtaagtcta tggtctggat ttgctacgaa atcttttcta
    14941 gatgagcgag atgttttacg ttctggtccg gaatgggatg gtttggtcgc ggaatttaag
    15001 ccgctttatc gtgagtactg tgagagaagt ttactgtctt cggatcttcg ggcggcgttg
    15061 gaaaatcaaa gggagcaaat tgttcagcgc gctgttgata gtagttcacc tgtttcgctt
    15121 agggattatg tatttttgtc gggaaatgta cctttcccgt cagaagagcg tatagggaaa
    15181 tgtcagtctg aaatagccat ggcggctgct caagatgctg tatatgaggg tggaagaaac
    15241 aagttagcat ttactagttt ggcggttctg tcaggtgtat tgtctgcagg gttcgcttta
    15301 gttagtgcgc gtagcgctgt tcgtacttac cgtcgccggg aagaggacga accggtgcaa
    15361 gggccgagcg cttctgcgcc aacgtcttct cactcttaag ctgcttttgc tattagctct
    15421 ttataggaat gctctgggcg cggacccatg tggccgatga cttcggcggc ggccagagcg
    15481 ccaagctttc cgcaggtttc aatgtctttc ccggatgtga atccatagag gaatccggca
    15541 gcaaactggt cacctgcgcc ggtggtgtcg atgacttcgg atacaggcgc ggcggatatt
    15601 tcataagttt tttcaccgtt cctaatgacc gcgccttttt cgctgcgcgt catggcggcg
    15661 atttcgacat gtttgctgac agcgctggcg gcatcttcaa aattatcctg catgaagagg
    15721 gattttattt cttcttcgtt ggcgaagaga atatcgatat ggctttctac cagccgcagg
    15781 aaatcggagc ggtggcggtc aacgcagaat gggtcggaca gcgtaagggc gacgcggtgg
    15841 ccggcggcgt gcgcggtttc ggcggcgtat ataaaggctt gtttagcggt gtcacgatcg
    15901 aacagatagc cttccagata agtgattttg gctttggcga tgagatcttc gtcaatatca
    15961 ccgggagtaa gttcaacact ggcgcctaga aaagtgttca tggtgcgctg ggcgtctggc
    16021 gttacgcaaa taaggcagcg gccggttttt gcacccataa tcaggggctg ggtatcaaat
    16081 tgaattccta aggagcgtaa atcatgttgg aatattctgc cgagatcgtc atccgcgact
    16141 ttgccgataa accctccttg tccgccgaaa gaagcaaatc ctgccatggt gttgccagcg
    16201 gagccgcctg agctttcgat accggctggc atttgggcgt aaagctctac ggcgcgggct
    16261 tcatctataa gggtcatcgc gcctttttcc atgccatagc gggcgttttg gtcctgaaca
    16321 aaggcttcgt cagtctggct gaggacatcg accaaagcat ttcctacggc gacgacatca
    16381 aattgcggtt gtgacattta ttcattcctt ttgtgattca taaaaaagct gttaaaagat
    16441 gtagcgaatt aagaacgccg cgccaaggag agagtttaag ccgatggcaa aaaaaactga
    16501 aaaagatttg aaaagcacaa ttgttttaaa agcgttagag caggccgagc agatgggctg
    16561 ggcgaatgtg gcgctttcgg atgttgcgga ggcggccgga gtgtcgctgg cggagctgtt
    16621 tgatttggtt gaggataagg cggatattct tgcgttgttt ggccggatga ttgaccggcg
    16681 cgttttggaa gctgtcagca aatcggatcc cagcgtttcc gaacgtgacc ggttgtttga
    16741 catcatgatg gagcgttatg aggctttgaa tgattaccgg gccgggttgg tttcaatttt
    16801 ggagagtttt cgttttgatc ccaagcaagc gcttatttcc gcgccgcatc tttgccgttc
    16861 gatgagctgg atgctggagg ctgcggggct tgagacatcg ggggtgcgcg gcgcgttgaa
    16921 agtcgcagga ctgacggggg tttatttgaa gactttgcgc gtgtggcgcg atgatgagag
    16981 tgcggatctg tccagggtta tggcggcact ggataaagat ttaggccgcg ccgagcaatt
    17041 tgccaatacg cttgggtttt agctttcttt ttgagcttgc agcgctttga tgcattcatc
    17101 gaccatgtcg ctaaaaggta tggcatcata ggcgccgtta accgaggcgg tgaggctggc
    17161 tgcgatgtga gcgcgttcga ttgctttttc gaggcttagg cccatatcta ttccggcggc
    17221 gaatgttccg cagaaggtgt cttcagcacc ttcggggtat ttccaattta aggcttcgtg
    17281 agcgggtttt ggtaccttgg atggcgggcc tccttctaca attaattcgc agcttccgtc
    17341 agtgcttatg aatatcgttg ttaagtgttt agccttcagt atgtgattga gcgctttttt
    17401 ggaggctgat ggtgcgatca gataatccag gttcgcaaga tcttcgtctg agatgtaggc
    17461 ttttggggca agatttaaga tcgttgtggc ttcagccttt ttggctgtgg ccagaagttt
    17521 ggagttttct tccatgttga tttcggtttg caacactaca gtgctatcgc tgtttaatgt
    17581 ttccatagga agttggtggg ctttggtata ggcgctggcc cccatggcca aaattgtgcg
    17641 attttcatcg acgattcgaa tggacgtacc ggtttgcagc ttctctgcct tggctacgcc
    17701 ggaggtgata acaccatgag tgcgcaagcg cagaaggata tgtttggcca gttcatcatc
    17761 ccccatttta ccggccagtg cggtttttgc acctgctttg gctgcggcca aggcttgcag
    17821 ggcccccttg ccgccgatgc tgtttttgta atgtttagcg tagtggacag cgccaaaagt
    17881 ttcttccggg tctgtggtga tgcggatgga ttgcaagatg gcgccgaata cgaaaatcat
    17941 gttttttgtg tctccagtct tgaaatccta cggttgtgtg ataagaaaat caacgaacgc
    18001 tagtcaagtg cagttgttcc agattatact aggatgcttt cttacgaaag ccttgtcttt
    18061 gcaagattat accgaggcgc atcagggagt gtaaaaaaat aaggataatg ccattatgac
    18121 cgatagattt gattaccata ctcattttga aggctgcgtt gaccaggtta aacaagaagg
    18181 gcgttatcgt tatttcgcta acttggaacg caaggttggg gattttcccc gtgcgatcta
    18241 ccgcatgccg aatggtcaat caaaagaagt tacgatctgg tgtaataatg attatctcgg
    18301 gatggggcag catccgaaga ttttggcagc ggcgcgcgcg gcactggaga attctggctc
    18361 tggcgcagga ggtacgcgca atatttccgg gacaacctgt tatgttgtgg agttagaaga
    18421 gtctgttgcc gatttacact ctaaagaagc ggggcttgtg atgtcttctg gctatgttgc
    18481 caatgaaggt gctttgggta cgctcggtaa attgatgcct gaatgcgctt ttttctctga
    18541 cgagcttaac catgcgtcga tgattcatgg aattaaggac agtaagcagc agaaatttat
    18601 ttatcgtcat aacgatatgg ggcatttgga aagtctgctg aaatctgttg atttggatcg
    18661 gccgaaaatt attgcttttg aatcggttta ttccatggaa ggcgatattt ccccgatgga
    18721 agaaatatgc gatttggcgg ataaatataa cgcatttacc tatctggatg aggtacatgc
    18781 ggtgggagtt tatggtccgc gtggtggcgg tgtttctgaa gagcgcggcc tgatggaccg
    18841 gattgatgtg attgaaggga cgtttggtaa agcttatggt gcgatgggcg gttttattac
    18901 cggcgatgcg gtcattattg atgcggtgcg ttcttatgcg ccgagtttta tttttaccac
    18961 tgcgctgccg ccggcagtat ctgcggcggc gatggcggcg gttgagcatc taaaaaattc
    19021 tgatgtagaa cgccagaggg tgcggcgaaa tgtgaagctg ctgaaagacg gtttgacgca
    19081 ggccgggcta aaattcctgc cgggggatag ccatatcgtg ccgctcattg tcggggagcc
    19141 gaattgctgc cgtgagctga cggatatttt gatgaatgat catgatattt atgtgcagcc
    19201 gattaattat ccgactgtgc ccagagggac ggagcggttg cgcttgacag ccacagccgc
    19261 gcattcggaa gaagatgttc tccatatggt ttctacgcta cggcatttgt gggaaaccaa
    19321 tcaccagctt atgcagattg cggcttaatc ttttttggga ttaattcctc gttgtttgtg
    19381 gcgtgatcgt catcgcgagc gtagcgaagc gatccagtgc gacaagaaaa aactctggat
    19441 tgcgtcatcg ccgttggcgg ttcgcaatga cgagatacct cgtttcttgg gg
//
LOCUS       DAOSWR010000018        19242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1796_length_19242_cov_4.935477, whole genome shotgun sequence.
ACCESSION   DAOSWR010000018 DAOSWR010000000
VERSION     DAOSWR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19242
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1796_length_19242_cov_4.935477"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK513_02480"
     CDS             <1..229
                     /locus_tag="PK513_02480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO81352.1"
                     /translation="FHGLGVPILLGTSRKSFIGKISNDEPPDDRLAGSLASALYGYSQ
                     GVQIFRVHDVAAHVQAFKVFDAIQNVQGAES"
     gene            complement(226..822)
                     /locus_tag="PK513_02485"
     CDS             complement(226..822)
                     /locus_tag="PK513_02485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO81353.1"
                     /translation="MIRHGETEANAARIMAGHIDTPLTAKGREQAYNAQKVINTLQVK
                     PAAIFHSHLSRARDTAIIINEALNAPMHEDHDLAEIHAGDWEGAPYEVIAEMFDGWPD
                     VPNGESHIDFFQRVKQGKTRALNRFNDPVLIVCHGGVMRAFGEIHGIPTPGKFQNAHL
                     YEFVPKPENPKFPWAIYDYRLCPKLKTLLKSESTIYQI"
     gene            complement(855..1646)
                     /locus_tag="PK513_02490"
     CDS             complement(855..1646)
                     /locus_tag="PK513_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HOO81354.1"
                     /translation="MKIKTVGIAGLGAIGSAVGRALIKGIDGLEWTAASNLETVDEFN
                     VPLVDFKTLAKSCDIIIECLPPSAVPELAKYVFAEQKTLILISSCALLMFPEILNAHK
                     NTHSRILVPSGALAGIDGVKGLKELGIEKSIIASTKKPSGFSGAPYVDKMGINLEQIQ
                     AKGIKTKIFEGNALEAAKGFPANVNVAATLSLAGTGPENTQVEIWADPNAKGNTHEIN
                     VYGQFSHLNVRITNMPDPANPKSSMLAAQSIVSVLKDMNTALVVL"
     gene            1718..2482
                     /gene="recO"
                     /locus_tag="PK513_02495"
     CDS             1718..2482
                     /gene="recO"
                     /locus_tag="PK513_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOO81355.1"
                     /translation="MECWQDQGIVLRARAHGEHGAIVSLLTAHHGRAAGYVRGGQGTK
                     LRGSLEVGNLVDVTWQSRTHDGLGAFTLELSKNVSAHLLQEPLKLAALQSACALCDAA
                     LPEREAHAGLYHGLQALFETLESDVWGAAYVLWEIALLKELGFSLDLTRCASGNGDEN
                     LMYVSPKTGKAVSASAGAPYKGKLLALAGFLKPGGGEMNEKEVMKGLEMTGHFLDTYV
                     FTHHSRGVPDARLIFAQRFAKTLDMQSIGKNEYAAG"
     gene            2469..4706
                     /gene="parC"
                     /locus_tag="PK513_02500"
     CDS             2469..4706
                     /gene="parC"
                     /locus_tag="PK513_02500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOO81356.1"
                     /translation="MPQDNSVPDHDVIDQPFGELLSDRYLSYALSTIMSRSLPDVRDG
                     MKPVHRRLMYAMYQLKLGPTGAPKKSARVVGDVIGKFHPHGDTAVYDAMVRLAQDFAQ
                     RYPLVDGQGNFGNIDGDNAAAMRYTEARLTTVALLLLDGIEENAVDFRPTYDGSENEP
                     VVLPGGFPNLLANGATGIAVGMATSIPPHNVAELCAAMECILEQKDVSIAELATIVSG
                     PDFPTGGTLIEDHRDIVSAYETGKGAFRVRAKWEREDLGRGKYQIVVNEIPYMVQKSK
                     LIERIAELVTTRKLPMLDDIRDESAEDIRIVLVPKSQNVDPNLLMEALYRNSDLENRF
                     SLNMNVLENGLVPRVMNLKEVLQAWMNHQKEVLVRRSQYRLEKVLHRLEVLEGYLIVY
                     LNIDEVIRIIRFEDDPKAELAKAFKLSEVQVEAILNLRLRRLHKLEEMEIKGEHENLS
                     AERDELVTLIGSDARQWTRIKKQIKALGKQFGQDTALGARRTVIGASPAPVAVDLDEA
                     MIEKEPVTIICSRQGWVRAMKGHGLNARDFKYKDGDREAFVLEAQTTDKLLVFGTNGR
                     FYTVGCDKLPPGRGFGEPLRIMVDLPNDSDIAAMMLHRPGQKILVAATSGHGFIVRSD
                     DVLAQTKNGKQVLNVSGNVEAVLCRAVADGHDHIAVIGKNRKMLVFELAEVPEMGRGK
                     GVILQKYKDGGISDAKTFKWDEGLEFKYGAGMTLVEDINPWVGKRASAGRLPPNGFPK
                     SNKFD"
     gene            complement(4912..5955)
                     /gene="motB"
                     /locus_tag="PK513_02505"
     CDS             complement(4912..5955)
                     /gene="motB"
                     /locus_tag="PK513_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOO81357.1"
                     /translation="MTDVAEEDKVEQPIIIKKIKKGGGHHGGAWKVAYADFVTAMMAF
                     FLLLWLLNVTTEDEKNAISNYFDPTHPKVSSDTSGAGGVLGGLSVAPKGAMVSNVQPI
                     TASTPSGSAVNNRSARKDNLEQAKEKLRKQEEERMQSAKAEIEKAIESDPELKELKDQ
                     LLIDMTPEGLRIQVIDKEGKPMFASGSARMFEKTRKLLEKVTEVIKKQPNELSVRGHT
                     DSVGYGPEATYTNWELSADRANSSRRVMLESGFPPERINNVVGKADTEHLIKDDPKNA
                     SNRRISIILLKEELTNPDFEKKAAAALPDIRKKTEEEREEEKAGDAYIEQPTGVFPKM
                     GTFKKTPGAIEFP"
     gene            complement(5988..6881)
                     /gene="motA"
                     /locus_tag="PK513_02510"
     CDS             complement(5988..6881)
                     /gene="motA"
                     /locus_tag="PK513_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOO81358.1"
                     /translation="MKFVGIALVFVCTFGGLLIAMHFDMPKFMKLVMLILGAMPGEFV
                     IIFGCAVAAFLIANSTETIKGTMKYFSALAKPSAYSKDDYVELMSMLFTVFKLARTKG
                     WLALEQHIEQPEESDVFAQFPSFQHNHHALIFLCDYLRIISLGNDSPHELEALMDEEI
                     ETIEHHEGHPGHAVQTMADGIPALGIVAAVLGVIKTMASISEPPEVLGKMIGGALVGT
                     FLGVWLSYGMVAPIAGAMTSKAETQVMYFKCIKVAVIAFLNGAAPQVAVEFARKFLPH
                     DIQPTFQELEEKLNELPSPGA"
     gene            complement(7103..8725)
                     /locus_tag="PK513_02515"
     CDS             complement(7103..8725)
                     /locus_tag="PK513_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO81359.1"
                     /translation="MTNPSETKKQRDRFLAFAFASADLFLEISMEGRITYALGASKSL
                     TGFEDEDIIGKKMLELFSVYDQAQIQNIYENAQAGKRAGPLIVDLNELVSKRKAVFTA
                     MRMPEDPNFYITLGFSNLLMSRLAGEISAISTGPILDRAGFIQQAKDTFDIARSIDQR
                     IEITLFDFTPTKEQKERLGKEGWNSFTESIGHFLQSQAIDGQAATMISDGKYGLIHEK
                     DIDSALIRERIESLAAKNDPEGEGVEVQSKTIHTNLNTLNNHDATRALVYTVNEFDRE
                     GTNFTIETLNSCAKDYVDANTERIKKLQSIIERQDFTLHFQPIVKLKTKEVSHYEILS
                     RFKEGDTMEWIMLGEDTGLAPAFDMAVCERAINHIKHKGGGSRTVFSVNLSSQSIQDD
                     TFKEKLMEKLGNQNNLHERLIFEVTESGHIKDRSKIQKFIEQLKSLKFTITLDDFGTN
                     AATIQHLQKLPVDYVKISGKYIRKILSSGRDAATVKNLTKIGKEHNIKVIAEFVEEQA
                     QADMLMEIGVDYGQGYLFGKPETAPSFTPPKK"
     gene            complement(8817..9878)
                     /gene="hemB"
                     /locus_tag="PK513_02520"
     CDS             complement(8817..9878)
                     /gene="hemB"
                     /locus_tag="PK513_02520"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOO81360.1"
                     /translation="MTAQKKNTVSAGAEQPSLPISQGAYPQTRMRRLRSQGWLRDMVC
                     ETRLSANDLIWPLFIIEGEDSAQTITAMPGVQRLTIDRALQAVKDAAALGIPAIALFP
                     ATPPEKKTPGGEESTNPFNLICRAVQAIKKECPNIGIITDVALDPYTTHGHDGLLEDG
                     KILNDASVEILCKQALNQARAGADIIAPSDMMDGRIGAIREALDSEDFEDVAILSYAA
                     KYASCFYGPFRDAVGSSGALKGDKKTYQMNPANTDEALREVALDLQEGADMVMVKPGL
                     PYLDIIQRVKSTFSAPTFAYQVSGEYAMLKAAAQSGAFDYKSALMESLLAFKRAGCDG
                     ILTYGALDAAKELKQQKTA"
     gene            9933..11006
                     /locus_tag="PK513_02525"
     CDS             9933..11006
                     /locus_tag="PK513_02525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOO81361.1"
                     /translation="MNQAVPRSEVLIETCGALALVTLNRPDAHNALSLEMIREIRAAL
                     NGWAEDESIEAVLFLGAGDKAFCAGGDIKAFYYSGMDCRRGLVTPRVPMVFFGEEYAL
                     NKQIFHYSKPTIAFMDGITMGGGYGIAGHCKVRIASAGTVFAMPEAGIGFFPDVGSMY
                     QLVRCPGNYGRYLALTGHHIDGKTMIAAGLADFYIEDAENLSQTVEQNLGCDDLGGAL
                     KTALRGVDDFDLNHADVIESAFTDCDVSAICGRLRENDDDFAFETLAILEMRSPISVM
                     VVAAYLDKAQRLGFDEIITMDLALCQKFILQQDMYEDIRAQLIDKDKSPQWCYQALGD
                     VSQALVDSYFTPCDYDLKDVRIF"
     gene            11116..11580
                     /locus_tag="PK513_02530"
     CDS             11116..11580
                     /locus_tag="PK513_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOO81362.1"
                     /translation="MNSTPYYEAIQLIERLHRHFLDVLKVELDKKGIQDINNVQSMIL
                     YNIGDDEMTVGELTIRGYYLGSNVSYNVKKMVENGYLVQERSVHDKRSIRVKLSEKGL
                     GLKTMISDMFARHENEISGSEVTDERLNALGDTMKMLERFWAKQTSAYGGGF"
     gene            complement(11804..12982)
                     /locus_tag="PK513_02535"
     CDS             complement(11804..12982)
                     /locus_tag="PK513_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOO81363.1"
                     /translation="MHKRADFNWEDPLLLDELLTDEERMIMQTARDYAQDKLLPRVIE
                     AYKTEEFDPNIMKEMGQLGLLGPMIDGYGCAGASYTATGLIARELERVDSAYRSCYSV
                     QSSLVMLPISLFGTEDQKQRFLPKLASGEYIGCFGLTEPDGGSDPNAMKTKAKKTKDG
                     YSISGAKQWISNSPIADVFIVWAKDEDAQMVGFILEKGMKGLSAPKIGGKLSLRAAPT
                     GGIAMDEVHVPEENRLPEAFSLKAPMLCLNSARYGIAWGVMGAAEDCWHRSRQYVMDR
                     EIFGKPLAAQQLIQKKLADMQTEITLGLAACHRLGRLRDCDRAAPEAISLLKRNNCGK
                     ALEIARLARDMHGGNGIADEYHVMRHMCNLEAVNTYEGTHDIHALILGRAQTGLPAFG
                     "
     gene            13028..13231
                     /locus_tag="PK513_02540"
     CDS             13028..13231
                     /locus_tag="PK513_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81364.1"
                     /translation="MSKELLNGREVILEFFPVGAFVKVSVMDTASLTEVSIQGPANAS
                     ELTLKRNALKRLEYVLKKKGLIT"
     gene            13257..13682
                     /gene="rpiB"
                     /locus_tag="PK513_02545"
     CDS             13257..13682
                     /gene="rpiB"
                     /locus_tag="PK513_02545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOO81365.1"
                     /translation="MKHKLAIACDHGGYSLKEAIKAHFSDVEWLDLGTNSNDSVDYPE
                     YGQKLAKAIAGGEVQQGIVICGSGIGISIAANRNPAVRAALCTDVTMARLTRAHNDAN
                     VLALGARIIGEEVAFDIVEIFLNTEFEGGRHRRRVDKLS"
     gene            13728..15017
                     /gene="glyA"
                     /locus_tag="PK513_02550"
     CDS             13728..15017
                     /gene="glyA"
                     /locus_tag="PK513_02550"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO81366.1"
                     /translation="MSGVVSGFFTDDLKEVDPEIYAATREELARQQNQIELIASENIA
                     SKAVLDAQGSVLTNKYAEGYPGRRYYGGCEFVDVVEDLARERAKQLFGAKYVNVQPNS
                     GSQANQGVMQALLQPGDTILGMSLAAGGHLTHGAAPNQSGKWFNSIQYGVRESDALLD
                     YDEVERLAKEHKPKMIIAGASAYPRQIDFKRFREIADAVGAYLMVDMAHYAGLIAGGS
                     YESPVPYADVTTTTTHKTLRGPRGGMILTNDLEIFKKINSAIFPGIQGGPLMHVIAGK
                     AVCFGEALKPDFKMYAKAIVENAKVLADVLKAGGADIVSGGTDSHIVLVDLRPKGLTG
                     KETEEALERAGITCNKNAVPFDPAPPMVTSGVRLGTPAATTRGFGPAEWKLVGEYIIE
                     ILDGLKANGEDGNGDVERAVKAKVEELCRRFPIYPTL"
     gene            15024..15494
                     /gene="nrdR"
                     /locus_tag="PK513_02555"
     CDS             15024..15494
                     /gene="nrdR"
                     /locus_tag="PK513_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOO81367.1"
                     /translation="MKCPFCGCEETQVKDSRPSEDNSVIRRRRFCPDCDERFTTFERV
                     QLREMTVLKSGGRKQPFDRDKIIKSMQIALQKRPIEIEQIEKTASAIVRKLESRGEQE
                     ISSDVIGAEVMNALKDLDAIGYIRYASVYKDFRKPEDFDDFLEDVKTLKSKSKK"
     gene            15494..16468
                     /gene="ribD"
                     /locus_tag="PK513_02560"
     CDS             15494..16468
                     /gene="ribD"
                     /locus_tag="PK513_02560"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOO81368.1"
                     /translation="MSPEIDEKYMRMALSMARRGLGMVAPNPAVGCVLVKDDVVIAAA
                     HTARGGRPHAEAIALEKAGYEAKGACAYVSLEPCSHTGQTGPCAQALIDVGVVRVVVA
                     CEDPDPRVSGRGIAMLNDAGVEVVEGVLEEEALALNQGFISRVIQRRPFVTLKCAVST
                     DGKIAAGAGERTQISGELSQRYMHLQRSLYDAILVGSETYLVDKPKLTTRLAGFEHES
                     LRVILDRRGRIKDASGFEICRQNTIEEVLAYLAEKGITRLLVEGGAQVHRSFLEAGLV
                     DEFQLCKSPLILGDRGVDAADFGAYSWLKLQKTRVLGEDMLEIYIRED"
     gene            16478..17071
                     /locus_tag="PK513_02565"
     CDS             16478..17071
                     /locus_tag="PK513_02565"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOO81369.1"
                     /translation="MFTGLVQDVGRVRSLDKDGDWRIVIETAALDLEEMSIGASIACS
                     GCCLTVVEKGSGWFAVDVSAESLSKTVIKNWQEGMRVNLEPSLKLGDELGGHIVFGHV
                     DGVAMLISIDVDGDSHRLKVKVPKELARFIAQKGSVVLDGISLTVNEVEGDLFGVNII
                     SHTWEHTNLSDKRDGDMLNIEIDMLARYVARMLEVAA"
     gene            17068..18180
                     /gene="ribB"
                     /locus_tag="PK513_02570"
     CDS             17068..18180
                     /gene="ribB"
                     /locus_tag="PK513_02570"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOO81370.1"
                     /translation="MSEAAMKTETALSSIDDILEDVRVGKPVILVDDENRENEGDLII
                     AAEMATADNINFMAKDGRGLICLPMERAMVERLGLELMGRGDNTHHRTAFTVSIEAKE
                     GVTTGISAADRATTIKAAINPQARREDIATPGHVFPLLARDGGVLERAGHTEAAVDLA
                     RMAGFSAAGVICEIMNDDGTMARLSDLVGFAGKHGLKIGTIEDLIAHRSARETLVKCI
                     HEDVLESRYGGRFRFILYANTVQYAEHVVLVKGDIARAKGPVLVRMHVVDFMSDILGA
                     NQDSDLHVSMKLIEDAGEGVIVILREPQPDALSRRLKGEVKKNNGGQLRRYGIGAQIL
                     KDLGIMKMILLTDHPKNVVGLEGYGLYITDYKSINP"
     gene            18189..18623
                     /gene="ribH"
                     /locus_tag="PK513_02575"
     CDS             18189..18623
                     /gene="ribH"
                     /locus_tag="PK513_02575"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOO81371.1"
                     /translation="MPDKPRILIVEARFYEDIADALVSGAIATLEAKGVAFDRISVPG
                     ALEIPAAIKFAARKYAGFVALGCVIRGETSHYDIVAGESARGLMDLSICDGLCIGNGI
                     LTCETRVQALERALPEKKNKGRAAALAALALAAHKARFAKGE"
     gene            18624..19097
                     /locus_tag="PK513_02580"
     CDS             18624..19097
                     /locus_tag="PK513_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO81372.1"
                     /translation="MSEEQKPASSDGASRKARALSARLSAVQALYQVSQNRQPMRVVL
                     DEYLHHRSGMEVQGERLVQPDEVLLKAILYGVEECRAELESVVDANLKKDAQDRVVEP
                     LLRSILICGAYELLIQNIDKPIIINDYLNVAHSFYERNEVALINGVLDSVASVFR"
BASE COUNT         4238 a         3737 c         5855 g         5412 t
ORIGIN      
        1 atttcatggt ttgggtgtac ctatcttgct cgggacgagc cggaagagct ttattggcaa
       61 aatttcgaat gatgaaccgc cagatgaccg cttagcgggt tcattggctt ctgcgcttta
      121 tggatattcg caaggcgtgc agatttttcg tgtgcatgat gtggccgcgc acgtacaggc
      181 gtttaaagtt tttgatgcga ttcagaatgt gcaaggcgca gaatcctaaa tttggtaaat
      241 cgtggattct gattttagta aagtttttaa ttttgggcag agacgataat cataaatagc
      301 ccaagggaat tttggatttt ccggctttgg tacaaattca tagagatggg cgttttggaa
      361 ttttccgggt gtggggatac cgtggatttc accaaaagcg cgcataacgc cgccatggca
      421 gacgatcaat acagggtcgt tgaaccggtt taaggccctt gtttttcctt gtttgacccg
      481 ctgaaagaag tcaatatgcg attctccatt cggcacgtcg ggccagccgt caaacatctc
      541 ggcaattacc tcatatggtg caccttccca atctccggcg tgaatttcag ccagatcatg
      601 gtcttcatgc atgggagcgt tcagagcctc attgatgatg atggcggtgt cgcgtgctct
      661 ggaaagatgg ctgtggaaga tcgctgcggg tttgacctga agtgtgttaa taactttttg
      721 ggcgttgtat gcctgttcgc ggcctttggc tgtaagcggg gtgtcaatgt gtccggccat
      781 gatacgcgcg gcgttggctt cggtttcacc gtgtcgaatc atataaaaac gctttgcggg
      841 gaggggcgca ggcattataa gacaaccaga gctgtgttca tatctttgag aacactgacg
      901 atgctttgcg cggcgagcat tgagcttttc ggattggctg gatcgggcat attggtgatg
      961 cgcacgttta ggtggctgaa ctgaccgtaa acattgattt cgtgagtgtt gcctttggcg
     1021 ttcgggtcgg cccagatttc gacttgtgta ttttccggac ctgtgccggc cagcgataat
     1081 gttgcggcga cattgacgtt ggccgggaag ccttttgcgg cctctaaggc gttgccttcg
     1141 aaaattttcg ttttgatccc tttggcttga atttgctcca gatttatccc cattttatcc
     1201 acataggggg caccggagaa acctgatggc ttctttgttg atgcaataat gcttttttca
     1261 attcctagtt ctttcaatcc tttaacacca tcaataccag caagcgcacc ggatggcacg
     1321 aggatgcgac tgtgggtatt tttgtgggca tttaaaattt caggaaacat caacaaagcg
     1381 caagatgaga tcagaatgag ggtcttttgc tcggcaaaaa catatttagc cagttcaggg
     1441 acggcggatg gcggcaggca ttcgatgatg atgtcgcagc ttttagcaag ggttttaaaa
     1501 tcaactaagg gtacgttgaa ttcgtcgacg gtttccagat tgctggccgc tgtccattca
     1561 aggccgtcaa tgccttttat taaagcacgg cctacggcgc tgccgattgc tcctaatcct
     1621 gcgataccga ctgtttttat tttcatggcc ggggattttt cctaatttca aaagatcctt
     1681 gctttcgcag ggatgatata tcggatagtt ttatttcatg gaatgctggc aagatcaagg
     1741 gatagtgctg cgtgcacggg cgcatgggga gcatggggcg attgtctcac tgctgaccgc
     1801 gcatcatgga cgcgcggccg ggtatgtgcg cggagggcag gggacgaagc tgcgcgggtc
     1861 tttggaggtc ggcaatctgg tcgatgtcac gtggcaaagc cgtacgcatg atgggctggg
     1921 ggctttcacg ctggaattgt ccaaaaatgt gtcggcgcat ctgttgcaag agccgttgaa
     1981 attggcggcg ttgcaatcgg cgtgcgcttt gtgtgatgcc gcgttgccag agcgtgaggc
     2041 gcatgcaggg ctttatcatg gtttgcaggc gttgtttgag acgctggaga gcgatgtgtg
     2101 gggcgcagcg tatgtgttat gggaaattgc actgcttaag gaactcgggt tttcgctgga
     2161 tttgacgcga tgcgcgagcg gcaatggtga tgagaatttg atgtatgtat cgccgaagac
     2221 gggcaaggcg gtgagcgcgt cggcaggggc gccctataaa ggcaagttgt tggcgttggc
     2281 agggttctta aaaccgggcg gcggggagat gaatgagaaa gaggttatga aggggttgga
     2341 gatgaccggg cattttctgg acacctacgt ttttacgcat cattccagag gcgttcctga
     2401 tgcacgcttg atttttgcgc agcgatttgc caaaactctt gatatgcaat cgataggaaa
     2461 gaatgagtat gccgcaggat aattcagttc cagatcatga tgtgattgat cagccgtttg
     2521 gggagttgct ctctgatcgg tatttgtcct atgcgctttc gacgattatg agtcgcagcc
     2581 tgcctgatgt acgcgatggg atgaagcctg tgcaccggcg tttgatgtat gcgatgtatc
     2641 agctcaagct tgggcccacg ggggcgccga agaaatctgc gcgcgtggtg ggcgatgtga
     2701 ttggtaagtt tcaccctcat ggtgatacgg ctgtatatga tgcgatggtg cgtttggcgc
     2761 aggattttgc gcagcgttat ccgctggtcg atgggcaggg gaattttggg aatattgacg
     2821 gggataatgc ggcggcgatg cgctatacgg aagcgcggtt gacgacggtc gctttgttgc
     2881 ttctggacgg cattgaggaa aatgcggtag attttcgccc gacctatgac gggtcagaaa
     2941 atgagccggt ggtgctgccg ggcggttttc cgaatctttt ggccaatggt gcgacaggga
     3001 ttgccgtggg gatggcgacc tccattccgc cgcataatgt ggccgagctg tgcgctgcga
     3061 tggagtgcat tttggagcaa aaggatgtca gtattgcgga gctggcgact attgtgagtg
     3121 ggccagattt tccaactggc ggcaccttga tcgaagatca tagggatatt gtcagcgcct
     3181 atgagacggg aaaaggggct ttccgcgttc gtgcgaagtg ggaacgggaa gatttagggc
     3241 gcggcaagta tcagatcgtg gtgaacgaaa ttccctatat ggtgcaaaaa tccaagctga
     3301 ttgagcgaat tgccgaactg gtgacaacgc gtaagttgcc gatgcttgat gatattcgtg
     3361 atgaatcagc ggaagatatc cgcattgtgc tggtgccgaa aagccagaat gttgatccga
     3421 atttgttgat ggaggcgctt tatcgcaatt ccgatctgga gaatcgtttt agtctgaata
     3481 tgaatgtatt ggaaaacggt ttagtgccgc gggtgatgaa cctgaaagag gttttgcagg
     3541 cctggatgaa ccaccagaaa gaggtgctgg ttcggcgttc gcaatatcgt ctggagaagg
     3601 ttttgcaccg gttggaggtg ttggaaggct atctgatcgt ttatctgaat attgatgagg
     3661 tgatccggat tattcgcttt gaggatgatc caaaggcaga attggccaag gcgtttaaac
     3721 tttccgaggt gcaggttgaa gcgattttga acctgcgttt gcgccgactt cataagcttg
     3781 aagagatgga aataaagggt gagcacgaga atctgtcggc agagcgtgat gagcttgtga
     3841 cgctgattgg gtcggatgcg cgtcagtgga cccgtattaa aaagcagatt aaggcgctgg
     3901 gtaagcagtt tgggcaggat actgcgctgg gcgcgcggcg cacggtgatt ggtgcgtcgc
     3961 cagccccggt tgcggtggat ctggatgagg caatgattga gaaagagccg gtgacaatta
     4021 tttgctcgcg tcagggctgg gtgcgggcga tgaagggcca tgggctgaat gcacgcgact
     4081 ttaaatataa agacggcgac cgggaggcgt ttgtgctgga ggcgcaaacg accgacaagc
     4141 tgcttgtttt tggcacgaac ggtcggtttt atacggtggg ttgtgacaag ttgccgccag
     4201 ggcgcggctt tggagagcct ttgaggatta tggtcgattt gcctaatgac agcgatattg
     4261 cggcgatgat gctgcataga ccggggcaga agattctggt ggcggcgacg agcgggcatg
     4321 gctttattgt gaggagtgat gacgtgctgg cccagaccaa gaacggtaag caggtgttga
     4381 atgtgtcggg caatgtagaa gcggtgctgt gccgcgcggt cgccgatggg cacgaccaca
     4441 tcgcggtgat cggtaagaac cgcaagatgc tggtgtttga gctggctgag gtgccggaaa
     4501 tgggacgcgg aaaaggcgtg atcctgcaga aatataaaga tggcgggatt agcgatgcca
     4561 agacctttaa atgggatgag gggcttgagt ttaaatatgg cgcggggatg actttggttg
     4621 aggatattaa tccgtgggtg ggtaagcgcg cctccgcggg ccgtttgccg ccgaatggtt
     4681 ttccgaagag taataagttt gattagtttt ttctattgtg aatggctaaa atgataccgc
     4741 caagaatgag agaggatatt gaacaaagga atgtgatcgc gattatttgt tttgcttcta
     4801 aattaaaggt caagaacaat agagactgga ggaagccgag agcgctaaac aggcagaaga
     4861 agccgagtaa tctgtcagtc cattcaggaa tttttttgga ttttttcata attaaggaaa
     4921 ctcaattgcg ccgggggttt tcttgaaggt acccattttg ggaaagacgc ctgtgggttg
     4981 ctctatatag gcgtcgccgg ctttttcttc ttcgcgttct tcttccgtct ttttgcggat
     5041 atccggaagt gcggcggcgg cttttttctc aaagtcgggg ttggtgagtt cttctttcag
     5101 caaaatgatg gagatgcgcc ggttgctggc atttttggga tcatctttga tgagatgttc
     5161 ggtatcggct ttgccaacaa cgttgttgat acgctcgggc gggaagccgc tttccagcat
     5221 gacgcggcgg gatgaattgg cgcggtcggc ggaaagttcc cagtttgtgt aagtcgcttc
     5281 ggggccgtaa cctacgctgt cggtatggcc gcgtacggag agttcgttgg gttgcttttt
     5341 gatgacttca gtgacttttt cgagcagttt acgggttttt tcaaacatcc gcgcgctgcc
     5401 ggaggcgaac attggttttc cttccttatc aatgacctga atgcgcaggc cttcgggggt
     5461 catgtcaatg aggagctggt ctttgagttc tttgagttcg gggtccgatt cgatggcttt
     5521 ttcgatttct gctttggcgc tttgcatgcg ctcttcttct tgtttgcgga gtttttcttt
     5581 ggcttgttcg aggttatctt ttctggccga acgattattt actgctgatc cggatggtgt
     5641 tgatgctgta ataggttgta cattactgac catcgcacct ttgggtgcaa cgcttaggcc
     5701 tccaaggacg ccgcctgcgc cgctggtgtc gcttgaaact ttggggtgtg tggggtcgaa
     5761 gtagtttgat attgcgtttt tttcgtcttc tgttgtgacg ttcaagagcc acaggagcag
     5821 aaagaatgcc atcatggccg ttacgaagtc ggcataggcg accttccagg cgccgccgtg
     5881 gtggccaccg ccctttttga tctttttgat aattataggc tgttcgactt tgtcttcctc
     5941 agccacgtcg gtcacttccc tttttacgtg ttattaagcc tgtggtctta agcgcctgga
     6001 cttggtaatt cgtttagttt ttcttccagt tcctggaagg ttggctgtat atcatggggt
     6061 aaaaatttgc gggcaaattc aacggcgacc tgtggtgctg caccgtttaa gaaggcgata
     6121 accgcgactt ttatgcattt aaaatacatg acttgcgttt cagctttgga ggtcatggct
     6181 ccggcaatgg gtgcgaccat gccgtaggat agccaaaccc caaggaatgt tcccactaac
     6241 gcgccgccga tcatttttcc taaaacttct ggtggttcgg agattgaggc catggtttta
     6301 attacaccca aaacggcagc aacaatgccg agcgcaggga tgccatcggc catggtttgc
     6361 acagcgtggc cgggatggcc ctcgtggtgc tcaatggttt caatttcttc atccatgagg
     6421 gcttcaagtt cgtgtgggct atcattgccc agggagatga tgcgcagata atcgcataga
     6481 aaaatcaggg cgtgatggtt gtgttgaaag cttgggaact gggcgaatac atcgctttct
     6541 tcgggttgtt cgatatgttg ttcgagagcg agccagcctt ttgttcgggc gagtttgaac
     6601 acggtgaaga gcatgctcat cagttcgaca tagtcatctt tggaataagc tgagggtttt
     6661 gcaagggctg agaaatattt catcgtgcct ttgatggttt cggtcgaatt cgcaatgaga
     6721 aatgcggcaa cggcgcagcc gaaaatgatg acaaattcgc cgggcatagc tccgagaatt
     6781 agcattacaa gcttcatgaa tttcggcata tcgaaatgca tggcgatcaa aagaccgcca
     6841 aacgtgcaga cgaatacaag cgcgatgccg acaaacttca tgacaagcaa aaatccttac
     6901 aattgcatgg atacgcagcg gtggaggttc tttaaaggat acattatttc ctgttaaaga
     6961 ctctttaatg gggcaacaaa actgcagaag aggtgtgttg cccgaagata tattttggcg
     7021 caaaaagtta aatctgtttg cactttagtc tggttattgt gcgtactttt ttttacaaga
     7081 tcaaggctgg aagctttgtg tgttactttt taggtggggt aaaacttggc gcagtttctg
     7141 gtttgccaaa taggtagcct tggccgtaat ccacacctat ttccatgagc atgtctgcct
     7201 gggcctgttc ttcaacgaat tcagctataa ctttgatgtt atgttctttg cctatttttg
     7261 tcagattctt aactgtggcg gcgtcacgtc cggatgaaag tattttgcgg atatatttgc
     7321 cgctgatttt gacgtagtcg acggggagtt tttgtaggtg ctggatggtt gcggcgtttg
     7381 tgccgaagtc gtccagagta attgtgaatt ttaatgattt aagttgttcg atgaattttt
     7441 gaattttact gcggtctttg atatgtcctg attcggtgac ttcaaatatc aggcgttcat
     7501 gcaggttgtt ttgatttcca agtttttcca tgagtttttc tttgaatgtg tcgtcttgga
     7561 tggattggct ggacagattt acagagaaga cggttcgtga tccgccgcct ttatgtttga
     7621 tatggttgat ggcgcgttcg catacggcca tatcaaaagc cggggctagt cccgtatctt
     7681 ctccaagcat gatccattcc atagtgtcgc cttctttgaa gcggctgagt atttcgtagt
     7741 gggatacttc tttggtttta agtttgacga tgggctggaa atgtaaggtg aagtcctggc
     7801 gctctatgat gctttggagt tttttaatac gctcagtgtt ggcgtctaca taatcttttg
     7861 cacaggagtt gagtgtctct atggtgaagt ttgtgccttc gcggtcaaat tcgttaactg
     7921 tataaacaag tgcgcgggtt gcatcgtgat tgttgagtgt attgaggttt gtgtggattg
     7981 ttttgctttg gacctcaacc ccttcacctt caggatcatt tttagcggcc agagattcta
     8041 tgcgttcgcg gatgagggcg ctatctatgt ctttttcatg gatgagtccg tatttgccgt
     8101 cagagatcat ggtggcggct tgtccatcga tggcttggga ttgaaggaaa tgaccaatgg
     8161 attcggtgaa gctgttccag ccttctttgc ccagacgttc tttttgctct ttggtcggtg
     8221 tgaagtcaaa caatgttatt tcgatgcgtt ggtctatgga gcgggcgatg tcaaatgtat
     8281 ctttagcctg ctggatgaat cctgcgcgat ctaaaatagg ccccgtgctg atggcggaga
     8341 tttcgccggc aagtctgctc attaaaagat tactgaagcc tagtgtgatg tagaagtttg
     8401 ggtcttcggg catgcgcatg gctgtgaaga cggctttacg cttgctgacc agttcgttga
     8461 gatcgacgat gagagggccc gcgcgtttgc ctgcttgtgc gttttcgtag atgttttgga
     8521 tctgggcctg atcgtagaca gagaagagtt cgagcatttt tttaccgata atgtcttcgt
     8581 cttcaaagcc ggtgaggctt ttggaggctc ccagagcgta ggtgatacgg ccttccatgg
     8641 aaatttccaa aaacaaatct gccgaggcaa aggcaaaggc cagaaagcgg tcgcgttgtt
     8701 tttttgtttc agaagggttg gtcatattgt ttgtatttta gaatccgcac tacttttaag
     8761 gatagcggaa aacgagtatc ttcgcattac atctttgtga tgcatacaga gattctttat
     8821 gcggtttttt gttgctttaa ctctttggcg gcgtcgagtg cgccgtaggt caatatgcca
     8881 tcacatccgg cgcgtttaaa ggccaaaaga ctttccatta gtgcactctt gtaatcgaaa
     8941 gcgccgctct gagcagcggc tttgagcatg gcgtactcgc cgctgacctg ataggcgaat
     9001 gttggtgcgg aaaaggtgct tttgacccgc tggataatat cgagataggg taggccgggt
     9061 ttgaccatga ccatgtcggc tccttcttgc agatcaaggg cgacttcacg cagagcttcg
     9121 tcggtattgg ccggattcat ttgataggtt tttttgtcgc ctttgagcgc gccggacgag
     9181 ccgaccgcat cacggaacgg accgtaaaaa caggaggcat attttgcagc gtaggagaga
     9241 atagcgacat cttcgaagtc ttcggaatcg agcgcctccc gaatagcgcc gatgcgcccg
     9301 tccatcatat cggatggcgc gatgatgtct gcgccggcgc gtgcctggtt gagggcttgt
     9361 ttgcacagaa tctcgacact ggcgtcattg agaatttttc catcttccag caaaccgtca
     9421 tggccgtggg ttgtgtaggg gtcgagcgca acatcggtga tgatgccgat gttggggcat
     9481 tcttttttga tggcctgcac ggcgcggcag atgaggttga acggattggt gctttcttca
     9541 ccgccgggtg tttttttctc tggcggggtg gcggggaaaa gggcgatggc ggggatgcct
     9601 aatgcggcag cgtctttaac agcttgcagc gcgcggtcga tggtgaggcg ctggacgccg
     9661 ggcatagcgg tgatggtttg tgcgctgtct tcgccttcga tgataaagag tggccagatg
     9721 agatcatttg ccgatagccg ggtttcgcag accatgtcgc ggagccagcc ttgtgagcgc
     9781 aggcggcgca tgcgtgtttg tggataggcc ccttgtgaaa tggggaggga tggttgctcc
     9841 gcgcctgcgc ttaccgtatt cttcttttgt gctgtcatgt tggcggttcc tgatattttt
     9901 tgtttccgcc tacatttaag cggtattacc ctatgaatca agcagttccc agatctgaag
     9961 tccttattga aacgtgcggt gccttggcgc ttgtgacgct taatcggccg gatgcgcata
    10021 atgcgttgtc gctggagatg attcgcgaga ttcgtgcggc gctgaatggt tgggcggaag
    10081 atgaatctat agaagcagtg ttgtttttgg gcgcaggaga taaagccttt tgcgcgggcg
    10141 gagatattaa ggcgttttat tattcgggca tggattgtcg gcgcgggttg gtgacgccgc
    10201 gtgtgccgat ggtgtttttt ggtgaagaat atgcgctgaa taagcagata tttcattatt
    10261 cgaagccgac gattgccttt atggacggga ttactatggg cggtggttac gggattgccg
    10321 ggcattgtaa ggtacggatt gcctcagcgg gtacagtttt tgccatgccg gaagcgggaa
    10381 tcggtttttt tcctgatgtt ggcagtatgt atcaattggt gcgctgcccc ggcaattatg
    10441 gacgttatct tgcattgacg ggtcatcata ttgatggaaa aacgatgatt gcggcgggtt
    10501 tggccgattt ttacattgag gatgcagaga atttatcgca aacggttgag cagaatcttg
    10561 gttgtgatga tttgggcggg gcgttgaaaa cggctctgcg tggggtggat gattttgatt
    10621 tgaaccatgc ggatgttatt gaatctgcgt ttaccgattg tgatgtttct gcaatctgtg
    10681 ggcgtttacg agagaatgac gatgattttg cgtttgaaac attggcgatt ttagagatgc
    10741 gttcgccgat aagcgtgatg gtggtggctg cttatctcga taaagcacaa agacttggat
    10801 ttgatgagat aatcactatg gatttggcgt tgtgtcagaa attcattttg cagcaggata
    10861 tgtatgaaga cattcgtgcg cagcttattg ataaagataa aagccctcaa tggtgttatc
    10921 aggcgttggg ggatgtttct caggctcttg ttgattctta ctttacgcct tgtgattatg
    10981 atttaaagga tgtacggatt ttctaagagg atcaggattt gtaagcttcc tcttgacctt
    11041 cttgacgcat cgcttcacac tgctatttgc gtaaaaaaca ataatgttta ttagatttat
    11101 tttggagtta aattcgtgaa ttcgacaccc tattacgaag ctattcagtt gattgagcgt
    11161 ttgcaccgtc attttttgga cgtgctgaag gtggaactgg acaaaaaagg tattcaggac
    11221 atcaacaacg tgcagagcat gattttatat aatattggcg atgatgagat gaccgtcggg
    11281 gaattgacga tccgtggcta ttatcttggc tccaacgttt cttacaacgt caagaagatg
    11341 gtggagaatg gctatctggt ccaggaacgc tctgtccatg acaagcgttc gattcgcgta
    11401 aagctgtcgg aaaaagggct ggggttgaag actatgattt cagacatgtt tgctcgccat
    11461 gaaaatgaga tttcaggttc tgaagttacc gatgagcgtc tgaacgcact cggcgatacg
    11521 atgaagatgc tggagcgctt ctgggcgaag cagaccagcg cctatggcgg tggattttag
    11581 atttttcttt atttttttcc gttcgcgccg ttaaaaacat acggggcccc ctatgaaacg
    11641 ggacggtgtt gccgcattca gctaagcttt tggttgcaaa tggcgtcttg gtgtttgctc
    11701 ttagccgtgc gccacttacc gtctgttttt ttacagacta tgcataatct tgatgtgatg
    11761 gttacatgga tttttgccag ggtcaaggaa aaaattccta tatttaacca aacgccggaa
    11821 ggcccgtttg agctcggcca aggataaggg cgtggatatc gtgtgttccc tcgtaagtgt
    11881 tgacggcctc gaggttacac atatgacgca tgacgtggta ttcgtcggcg atgccgttgc
    11941 cgccgtgcat gtcgcgggcg aggcgggcga tttccagcgc tttaccgcaa ttattacgct
    12001 ttagaagtga aatggcttcg ggggcggcgc ggtcacaatc acgcaggcgg cccaaacgat
    12061 ggcaggcggc gaggcccagc gtaatttcgg tttgcatatc ggcgagtttt ttctggatga
    12121 gttgctgcgc ggccagcggt ttgccaaaga tttcacggtc catgacatat tggcgggagc
    12181 ggtgccagca atcttcggct gcgcccatga cgccccaagc gattccgtaa cgcgcggagt
    12241 tgaggcagag cattggggcc ttgagcgaga aggcttcggg caggcgattt tcttcgggca
    12301 cgtggacctc atccatggcg atgccgccgg tgggagcggc gcgtaaggaa agcttgccgc
    12361 cgattttggg cgcggacagg cctttcatgc ctttttccaa aataaagccg accatttggg
    12421 cgtcttcgtc tttggcccag acgatgaaga catcggcgat gggtgagttg gaaatccatt
    12481 gtttggcgcc ggagatggag tagccgtcct tggttttctt ggctttggtt ttcatggcgt
    12541 tcgggtcgga gccgccatcg ggttcggtga ggccaaagca gccgatatat tcgccggagg
    12601 cgagtttggg caggaatctc tgtttttgat cttcggtgcc gaagagagaa atagggagca
    12661 taacgaggga agattgcaca gaatagcagg agcgatatgc ggaatcgacg cgctccagtt
    12721 cacgggcgat gagcccggtg gcggtgtagg aggctcccgc gcagccatag ccatcgatca
    12781 tcgggccgag cagaccaagc tgccccattt ctttcatgat attgggatca aattcttcgg
    12841 ttttataggc ttcgatgacg cgcgggagca gtttatcctg agcgtaatcg cgcgcagttt
    12901 gcatgatcat gcgttcttcg tcggtgagga gttcatcgag gagcagcggg tcttcccagt
    12961 taaagtcggc gcgcttatgc atgattgtct ctttcttgat acggtatgtt tcgctataga
    13021 gaatatcatg agcaaagaac ttctcaatgg ccgtgaggtg attctcgaat tttttcccgt
    13081 tggggctttt gtcaaagtct cggtgatgga tacggccagc ttgacggaag tttcgattca
    13141 ggggcctgcg aatgcatcgg aattgacgct taagcgaaat gctttgaagc gccttgaata
    13201 tgtgctgaaa aagaagggct tgattactta agggtgtgca cactatatat tgtggcatga
    13261 aacataaatt ggcgattgcc tgtgatcatg gcggatactc tttgaaagaa gcaatcaaag
    13321 cgcactttag cgatgttgag tggcttgatc tgggcacgaa ttctaacgac tcagttgatt
    13381 atcctgaata tgggcaaaaa ctggccaagg cgattgcagg tggcgaggtc cagcagggta
    13441 ttgtcatttg tggcagcggg atcgggattt cgattgcggc gaaccgtaac cccgcggtga
    13501 gggcggcgtt gtgcacagat gtaaccatgg cgcgcttgac ccgggcgcat aatgatgcca
    13561 atgtgctggc ccttggcgcg cgaattatcg gcgaagaggt ggcgttcgat attgtcgaga
    13621 tatttttgaa tacagaattt gaaggggggc gtcacaggcg gcgcgtggat aaacttagtt
    13681 aggaaaggtt ttagttaatg agcaatgcag caatgaaaga gaataaaatg agcggtgtgg
    13741 tgagcggatt ttttacggat gatttgaaag aggttgatcc ggaaatttat gcggcgacac
    13801 gcgaagaatt ggcgcgtcag caaaatcaga ttgagctgat tgcttctgaa aatattgcct
    13861 caaaggctgt tttggatgcg cagggatctg tgctgaccaa taaatacgct gaagggtatc
    13921 cggggcggcg ttattatggc ggctgtgagt ttgtggatgt ggttgaggat ttggcgcgtg
    13981 agcgtgcgaa gcaactgttt ggtgctaaat acgttaatgt acagccgaat tccggttctc
    14041 aggccaatca gggtgtgatg caggctttgc tgcagcctgg tgatacgatt ttggggatga
    14101 gtcttgcggc cggcgggcac ttgacccatg gcgcggcgcc gaaccaatcc gggaaatggt
    14161 ttaactctat ccagtatggc gtgcgtgaaa gtgatgcttt gctggactat gatgaagttg
    14221 agcgtttggc gaaagagcat aagcccaaga tgattatcgc cggggcttcg gcctatccgc
    14281 gtcagattga ctttaaacgt ttccgtgaaa ttgccgatgc tgttggtgca tatttgatgg
    14341 tggatatggc ccattatgca ggactgattg cgggtggttc ttatgaaagt ccagtgcctt
    14401 atgctgacgt tacgacaacg acgacgcata aaactttgcg tgggccgcgt ggcgggatga
    14461 ttctgactaa tgatctggag attttcaaaa agatcaattc ggcaattttc ccgggcattc
    14521 agggcgggcc tttgatgcat gttattgccg gtaaggccgt ttgttttggc gaagcgttga
    14581 aacctgactt taagatgtat gcgaaggcta ttgttgaaaa tgccaaggtt ttggccgacg
    14641 ttttgaaggc gggcggtgcg gatattgttt ccggcgggac agacagccat attgtgttgg
    14701 tcgatttgcg tccgaaaggt ctcaccggta aagaaaccga agaagcgctg gagcgtgcag
    14761 gcattacttg taacaagaat gctgtgccgt ttgacccggc gccgccgatg gtgaccagcg
    14821 gcgtgcgtct gggaacgccg gcagcgacaa cgcgcggctt tggcccggct gaatggaagc
    14881 tggtcggtga gtatatcatt gaaattcttg atgggcttaa ggccaatggg gaagacggta
    14941 atggtgatgt tgagcgtgct gttaaagcta aggtagaaga gctgtgtagg cgattcccga
    15001 tttatccaac cttgtaggtt tatatgaagt gcccgttttg cggatgtgaa gaaacacaag
    15061 tgaaggattc tcgcccgagt gaggataatt ccgtaattcg ccgtcgccgg ttttgtccgg
    15121 attgtgatga gcgttttaca acgtttgaac gggtgcagct ccgcgagatg acggtactga
    15181 aatccggtgg acgcaagcag ccctttgatc gcgacaaaat tattaaatct atgcaaattg
    15241 cgttgcagaa gcgtccgatt gagattgagc agatcgaaaa gacggccagc gcgattgtac
    15301 gtaagctgga atcacgcggc gagcaggaaa tttcttcaga tgtgattggg gctgaagtga
    15361 tgaatgcgct caaagatctc gatgcgattg gctatatccg ttatgctagc gtgtataaag
    15421 atttccgcaa gcctgaggat tttgatgatt ttctggaaga tgtgaaaacg cttaagtcta
    15481 aaagtaagaa ataatgtctc ctgaaattga tgaaaaatat atgcgtatgg ctttgagtat
    15541 ggccaggcgg ggtttgggca tggttgcgcc gaatccggct gtcgggtgtg ttttggtgaa
    15601 ggatgatgtt gtgatcgcgg cggcgcatac ggcacgtgga gggcggccgc atgcggaggc
    15661 gattgctttg gaaaaggccg ggtatgaggc caagggcgcg tgcgcgtacg tgtctttgga
    15721 gccttgttcg cataccgggc aaacgggccc ttgtgcgcag gcgttgattg atgtgggcgt
    15781 tgtgcgggtg gttgtggcgt gtgaagatcc tgatccgcgg gtttcagggc gcggaattgc
    15841 aatgctgaat gatgcgggtg ttgaagtggt tgagggtgtt ttggaggagg aggctttggc
    15901 tctgaaccaa ggttttatca gccgggtgat acagcgacgt ccctttgtga cattgaaatg
    15961 tgcagtttca actgatggta aaattgctgc tggtgccggt gagcgaacgc aaataagcgg
    16021 agaactgtcc cagagatata tgcacctgca acgctccttg tatgatgcga ttttggttgg
    16081 ttcagaaacg tatttggttg ataaaccaaa gttaacaacg cggctggctg gttttgagca
    16141 tgaatctttg cgggtcattt tggatcgacg cgggcgtatt aaggatgcgt ctggctttga
    16201 aatttgtcgt cagaatacga ttgaagaggt tttagcctat ttggccgaaa aaggtataac
    16261 gcggttgttg gtggaaggcg gagcacaagt gcatcggagt tttttggaag cagggctggt
    16321 ggatgaattt caattgtgta aatcgccgct gattttggga gatcgggggg tggacgcggc
    16381 agattttggt gcgtattcat ggcttaaatt acaaaaaacc agggttttgg gcgaagatat
    16441 gctggaaatt tatatccgag aagattaggg tggccgcatg tttacaggac ttgttcagga
    16501 tgttggccgt gtgcgttccc ttgataaaga tggcgactgg cgtattgtga ttgaaacggc
    16561 ggcgctggat ttggaggaga tgtcgattgg cgcatcaatt gcgtgttcgg gctgttgttt
    16621 gactgttgtt gagaagggtt ctggctggtt tgccgttgat gtgtcggcgg agagcctttc
    16681 gaaaacggtt attaaaaact ggcaagaggg catgcgggtt aatctggagc cgtctttgaa
    16741 gctgggcgat gagttgggcg gtcatattgt tttcgggcat gttgatgggg tggcgatgtt
    16801 gatatccatt gatgttgacg gggattcgca tcgtcttaag gttaaggtgc cgaaagagct
    16861 tgcgcggttt attgcgcaga aaggttctgt ggttttggat ggaatttcat taacggttaa
    16921 tgaggttgaa ggggatttgt ttggtgtcaa tatcatttcc catacctggg agcatacgaa
    16981 tctttcagat aagagagacg gtgatatgct caatatcgag attgatatgt tggcgcgcta
    17041 tgtggccaga atgcttgagg tggcagcatg agtgaggcag cgatgaaaac ggaaacagcg
    17101 ctttcgagca ttgatgacat tttggaagat gtgcgtgtgg gcaagccagt tattctggta
    17161 gacgatgaaa atcgtgagaa tgagggcgat ctcatcattg ccgctgaaat ggccacagcg
    17221 gacaatatta attttatggc caaggacggg cgtggtttga tttgtctgcc gatggaacgg
    17281 gcgatggtgg agcggctggg gctggaattg atggggcggg gggataatac gcatcatcgc
    17341 actgccttta cggtgtcgat cgaagccaag gagggggtaa cgacggggat ttcggctgcc
    17401 gaccgggcga cgacaatcaa ggcggccata aacccgcagg cgcggcgtga ggatattgct
    17461 acgccggggc atgtgtttcc attgctggcg cgtgacggcg gggttctgga gcgcgcgggg
    17521 catacggaag cggcggttga tctggcgcgg atggcaggat tttctgcggc gggtgtgatt
    17581 tgtgagatta tgaatgacga tggcacgatg gcgcgtctgt cggatttggt tggttttgcg
    17641 ggtaagcatg ggctgaaaat cggtacgatt gaagatctga ttgcgcatcg cagtgcgcgc
    17701 gaaacgctgg ttaaatgtat tcatgaagat gtgctggaga gccgttatgg tgggcgtttt
    17761 cggttcatac tgtatgcgaa tacggttcaa tatgccgagc atgtggtgct ggtgaagggc
    17821 gatattgcgc gggccaaggg accagtattg gtgcggatgc atgtggttga ttttatgagc
    17881 gatattttgg gggcaaatca ggattctgat ctacatgttt ctatgaagct tattgaagat
    17941 gctggagagg gtgtgattgt gattttacgc gagccgcagc cggatgcttt atctcggcgc
    18001 ttgaagggag aggtgaaaaa aaataatggc ggtcagcttc ggcgatatgg gattggtgct
    18061 cagatattga aagatttggg tattatgaag atgattcttt taaccgatca tcctaaaaat
    18121 gttgttggtt tggaagggta cggattatat ataactgatt acaaatcgat taacccttag
    18181 tgctcaaaat gcctgataaa ccccgcattc ttattgttga agcccgattt tatgaagata
    18241 tcgccgatgc gttggtgagt ggggctattg ctacgcttga agcgaaaggt gtggcttttg
    18301 accggatttc tgttccgggc gctttggaaa ttccggcggc tattaaattt gccgcgcgca
    18361 aatatgccgg atttgtggcg ttggggtgcg tcatccgcgg agaaacctcg cattatgata
    18421 ttgtggccgg ggaaagcgcg cgaggactga tggatctttc tatctgtgac gggctgtgta
    18481 ttggcaacgg gattttgaca tgtgagacac gggtacaggc tttggagcgc gcacttcctg
    18541 agaaaaagaa taaaggccgg gcggcagcgc tggcggcgct ggcgctggcg gcgcataagg
    18601 cgcgttttgc gaagggtgaa tagatgagcg aagaacaaaa acctgcatca tcggacgggg
    18661 cttcgcgcaa ggccagggcg ctttctgcgc ggttgagcgc tgtgcaagcg ctttatcagg
    18721 tcagccagaa taggcagcct atgcgggttg ttttggatga gtatttgcat catcgctctg
    18781 ggatggaagt tcaaggtgag cggttagtgc agccggatga agtgcttttg aaagcgattt
    18841 tatatggcgt agaggagtgc cgtgcggaat tggaatcggt tgtggatgct aatttgaaaa
    18901 aggatgctca ggaccgtgtt gttgagccgc ttttgcgttc aattctgatt tgcggggcct
    18961 atgagctttt gatccaaaat attgataagc ctatcataat taatgattat ttgaatgttg
    19021 cacattcttt ttacgagcgt aatgaagtgg cgttgattaa tggcgtttta gatagcgttg
    19081 cttctgtgtt tcgataggcg catttactat gttactgcgt ttttattcca taaaatttta
    19141 tttaattcta acctgccgtt aaatcttttt ctttaccata aattgtaggg ttcttataag
    19201 cattagggtg taaaaaaatg cgaatgatat ttatagcgtt ag
//
LOCUS       DAOSWR010000019        19216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1803_length_19216_cov_6.123584, whole genome shotgun sequence.
ACCESSION   DAOSWR010000019 DAOSWR010000000
VERSION     DAOSWR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19216
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_19216_cov_6.123584"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="PK513_02585"
     CDS             <1..1404
                     /locus_tag="PK513_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOO81373.1"
                     /translation="GHTPIPYKEVAGTGKSQVTFDRVTIEKALDYAAEDAEVTLRLYK
                     LLKPRLALEKMVSVYENIERPLIPVIAQMELEGIKVDPAILKSMSTDFGKRIADLEEE
                     IHKLAGTPFNIASPKQIGEILFDQMGLDSGKKTKTGQHSTDVKTLEELALQGHEIVQK
                     ILDWRGLSKLKSTYTDALQDQVVAETGRVHTSYHMTGTSTGRLASSDPNLQNIPIRTE
                     DGRKIREAFIADTGCVLLSVDYSQVELRLAAEMAGVEALQRAFKDGVDIHTLTASQVF
                     DVPLDNVDSETRRRAKAVNFGIIYGISGWGLAKQLGYDPADAADFIKRYLARFPEIKD
                     YMEEKKDEARANGFVKTLYGRKCFTPNINAKIPAQRAGSERAAVNAPLQGTAADIMKI
                     AMAKMPVALERAGLKAKMLLQVHDELIFELPVEELEATSKLVREIMENAWKIMGVEIS
                     VPLIAEAGSGHSWAEAH"
     gene            complement(1414..2256)
                     /locus_tag="PK513_02590"
     CDS             complement(1414..2256)
                     /locus_tag="PK513_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOO81374.1"
                     /translation="MVRPTNLKDIQFTALRGRVARFIENKTFTNFIIAIIIVNAAILG
                     LETDSSVMTRYSDVLVYLDEIALTIFVVELALKLFVYRLSFFRQGWNVFDFVIVGIAL
                     IPASGPLAILRALRILRVLRLVSVVPSMRRVIAALFHAIPGMASIMAVLLIIFYVASV
                     LSTKLFGQTEEFAMFFGTIGASMYTLFQIMTLEGWSEEIVRPVMEVYPYAWVFFIPFI
                     ITTSFAVLNLFIGIIVDAMNIIHEKEEYKGPERRKNNIVSGNEIIALHREMAELKELI
                     RKKS"
     gene            2362..3129
                     /locus_tag="PK513_02595"
     CDS             2362..3129
                     /locus_tag="PK513_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81375.1"
                     /translation="MNETIQILVGQSQYIRASFAKPKEHLMQSVDRLLVIMVHGFPGD
                     KNACNNIFVDLEHVLSEKRYHTLRFDFRGCGASDGLAEDFTLYSASEDFETVIKWAKD
                     RGYERFVFICDGLGASIALMNAPKEARCYVLLWPMVDLPMIAQHVFQAEEIEEDWKKA
                     GYIMKEDSRIGIPFLQQLKKAKMTKILKDLGKPLLIMHGAQDKVSPIEQLDMFRACAG
                     HRRVEITSFQDGTHGLPQENHRKSMFFHIRQFIEKYT"
     gene            3234..3428
                     /gene="rpmI"
                     /locus_tag="PK513_02600"
                     /pseudo
     CDS             3234..3428
                     /gene="rpmI"
                     /locus_tag="PK513_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
     gene            3486..3863
                     /gene="rplT"
                     /locus_tag="PK513_02605"
     CDS             3486..3863
                     /gene="rplT"
                     /locus_tag="PK513_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOO81376.1"
                     /translation="MARVKGGPRAHARHRKVIKAAKGYYGRRKSCFRTAVQAVEKAGQ
                     YAYRDRRAKKREFRKLWIQRINAAARLHDMTYSTFTHGLKLAGIELDRKSLSEIAIHD
                     EKGFAAIAKQAKDALGKAEKAVA"
     gene            complement(4209..4874)
                     /locus_tag="PK513_02610"
     CDS             complement(4209..4874)
                     /locus_tag="PK513_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81377.1"
                     /translation="MTTKACHLSQAEILEKLLIAYKEHEGIVYALKERKIVCLVRLGV
                     INNYANIKSDLEQKMPEQACRVLARKMSPTGLKQIQIDLLDKEDGQSINMFREREERK
                     KNILMVADDDMFVRKAMQKILGFYGDVVEVDSGDKVVSEYLRHNPDVLLLDIHMPGKS
                     GLELIDKIIEVDTDAFIIVFSADSVADNVLQAIEKGAVGFVSKPPKKERIMDYLNRCI
                     TIH"
     gene            complement(4871..6955)
                     /locus_tag="PK513_02615"
     CDS             complement(4871..6955)
                     /locus_tag="PK513_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HOO81378.1"
                     /translation="MLHEPQSFLEHFFLFGDIPPNAYYGTYAIPLVILSYVIASLGSF
                     TGLRLATDIHKAKTEKQKSYLHIGGAFAFGAGIWSMHFIGMLAYNMDMVHTYDPFLTF
                     VSMIIAVAIAYGVLQIIRTGKIEILQLVISALLLGTAICGMHYTGMAAMEMDADLRYT
                     PGLFSLSALIAVTASGAALFIVFMLGQHEGRGKTFWQAAAALIMGAAICGMHYTGMAA
                     SVFIPYADCRYDSVQSYNALAMIVAITSSTIFAITIILSLYKPTEGKASEVEDNQYSG
                     NAVFLQLSSLLSLFLVLLVGSYFFSNASVSEKKNDSALLNAASLQRLLLVHYTHQTSI
                     IMATHAAHNWKEAAQNNKIVQQDEKHIEANYKGLLEGGMVILSVDGNKTLEITPFNDK
                     DTRSALLTAQKEWGRLKHLAVTTLQSDIKTIINTPQNDELATQLRITLEAQDESVKSI
                     QKYLEQKSQNLEIKQQIILGIGFLVFLSTVLYARFFIANPIEKARKDLKNSRDNLEKR
                     VEEQTADLRKSKEDAEHLNAIIEKEKTYLDAILNNMMQGVITIDKNGIIQTFNQWAEF
                     IFSYQAHEAIGKNVSMLMPEPHHSQHDQYLKNYLETGESHILDTGVDVEGLRKGGQTF
                     PMKLSVTEIKNDHETTFLGLTLDISEQKEKENALKEAKRVAEEASRAKSDFLANMSHE
                     IRTPMNAILGMS"
     gene            complement(7202..8431)
                     /locus_tag="PK513_02620"
     CDS             complement(7202..8431)
                     /locus_tag="PK513_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOO81379.1"
                     /translation="MDILIEELEGSLWAAALKNGRLEGLEVDPPYEEIRWGSIYWAKV
                     KTIDKALDAVFVDLDGDNTGILYNADVRWRDDDGKVHKGGDESISKRFQPGDMVALQA
                     KTAYLSNDQDDFTPSENKIPQMSMDITLPGRYLIYCANMDCNRISQRIRDKKLRKQLH
                     KMMDNMVDIQGCILRLAAAHTQSDVLIREGKILKSAWEEIQKYFEGPEPGLIALGPDA
                     IQRTLSDQASQAIDRIEIVTMEHFKHIEEWCSIFAPDLVTKIDPIELDNASEDLALFH
                     YRDIIGQIEDLFHPYALLPGGGNIIIQGTAALTAIDVNKGGSRASHLDVNIEAAREAA
                     RQIRLRNCGGIIVIDFLKSQDRKDQGKLLAALEAAIDEDPCTVQIHGITALGLIEITR
                     KRRTPPLADRFQGMLIE"
     gene            complement(8453..9034)
                     /locus_tag="PK513_02625"
     CDS             complement(8453..9034)
                     /locus_tag="PK513_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOO81380.1"
                     /translation="MKNKLILASASPRRLDLLKQIGIIPDKILPADIDETPLKGEHPH
                     DLAQRLAREKAAAIAKKNPGNFILAADTVVGAGRKILDKAEDKPYARHCLESLSGRRH
                     HVYGGIALIAPTGKTVHRLIDTLVQFKRLTPSEIDQYLHFGQWRGKAGGYAIQGYAES
                     YIKFIRGSYSNVVGLSLYDTMRVLEGAGFKTDA"
     gene            complement(9024..9242)
                     /gene="infA"
                     /locus_tag="PK513_02630"
     CDS             complement(9024..9242)
                     /gene="infA"
                     /locus_tag="PK513_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOO81381.1"
                     /translation="MAKEDLMEFKGVVSEILPNAMFRVTLENDHVILAHTAGKMRKNR
                     IRVLQGDVVIVEMTPYDLTKGRIIYREK"
     gene            complement(9372..9866)
                     /locus_tag="PK513_02635"
     CDS             complement(9372..9866)
                     /locus_tag="PK513_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HOO81382.1"
                     /translation="MTKPSKTAIKSIRIENITAAPEGTLIARDRDIALRDLEAASRFI
                     PFKDTAGPYDVCLTLEENRLVFRIQNAQGNKLPMLVLSLKPYRRLIKDYFLIVRSYDA
                     AVQDGKPSRIEAIDMGRRGLHNEGAGLLIERLEDKIEMDEDTARRLFTLICVLHSAHA
                     PALW"
     gene            complement(9863..10522)
                     /gene="hisG"
                     /locus_tag="PK513_02640"
     CDS             complement(9863..10522)
                     /gene="hisG"
                     /locus_tag="PK513_02640"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOO81383.1"
                     /translation="MTTDRKLILAVPKGRILEELMPLLSACDIVPEDDFMSDSSRKLS
                     FSTNHADLDIIRVRAFDVATFVAYGGAQIGVVGSDAIEEFSYDELYAPVDLGIGKCRL
                     SVAMPDADAKKQNTVHESHIRIATKYPSLTARHYARSGIQAECIKLSGAMEIAPSLGL
                     CRRIVDLVSTGSTLKANGLTETETILEISSKLVVNRSAVKTDAERIGHWVNAFQSAVQ
                     K"
     gene            10648..11010
                     /locus_tag="PK513_02645"
     CDS             10648..11010
                     /locus_tag="PK513_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81384.1"
                     /translation="MDILIVDDNAFILEIISKMLAGQGYKIHPKSNVDEAISYLEGGS
                     ECDLVITDIVMPEKDGTKLASYVKSKTPSMPVLAMTGGLENALEDYVVLAEMFADEVM
                     TKPFHKNQLIETIERLVA"
     gene            complement(11104..11880)
                     /locus_tag="PK513_02650"
     CDS             complement(11104..11880)
                     /locus_tag="PK513_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81385.1"
                     /translation="MSLTNALENLFCDRTAENVLAYEHARDQADRGFFRLLTKDPQTQ
                     TNGILRCYVRKTRESINALINDQEIQDLCQSTYETDVPEIFDILKAYQDGKKLQAFKM
                     AHKLQDLPEDVRTRQSDLVIKAYRSNQPWAIKYRKILQEAGATRPPHAFSQHTQQAKP
                     NTKFVSPCPEIERMPKNIRNVLFAYGNENTTKAYSLIDTFSVINPQQNRIESDCVIKN
                     LRNGSSWAKAFAKIAREANTARFTRQHQKPLNILEFSRTL"
     gene            complement(11840..12082)
                     /locus_tag="PK513_02655"
     CDS             complement(11840..12082)
                     /locus_tag="PK513_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81386.1"
                     /translation="MSGAVAFNNLSFDKEQEDENYKIREMEVPESYIPLIDALQKQIT
                     NQHKNWTGENEKPKAVSWIGKVFFEPYECFRESFLR"
     gene            complement(12180..13433)
                     /gene="murA"
                     /locus_tag="PK513_02660"
     CDS             complement(12180..13433)
                     /gene="murA"
                     /locus_tag="PK513_02660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO81387.1"
                     /translation="MKVVGGRPLHGDIPISGAKNCALKLMCAALLTDQSLYFENSPNS
                     LRDMNSQTELLEYLGCAVKREGPLMAINAGPVHTQTAPYDIVRKMRGSILVLGPLLAR
                     FGEAKVSLPGGCAIGTRPVDMHIKALEELGAKITLEEGYIHATAPQGGLQGTKILFPK
                     VSVGATENLLMAATLSNGETVLANAAREPEITDLGECLIKMGAKITGLGTDTIRIEGV
                     SHMHGAHHAIVPDRIETGTYMVAAAMTGGSLRLQNAHLEHLSAAAGHLSQTGVEIDQD
                     GSDIIVSRDPECPLKAIDIMTEPHPGFPTDLQAQFMAMLTIAEGAGLVTETIFENRFM
                     HVPELNRMGANITVQGHSAIVRGVPKLTGAEVMATDLRASVALVLAGLVAEGETTINR
                     IYHLERGYENIVEKLSACGAQIKHA"
     gene            complement(13483..14043)
                     /gene="dcd"
                     /locus_tag="PK513_02665"
     CDS             complement(13483..14043)
                     /gene="dcd"
                     /locus_tag="PK513_02665"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOO81388.1"
                     /translation="MPPGLLADHQIRELARSQGMIEPFEEKQKREGNISYGLSSYGYD
                     ARCAADFKIFTNVDNAIVDPKDFSAQSFVDRETNVCIIPPNSFVLTHTVEYFRIPKDV
                     LVICLGKSTYARCGLIVNVTPLEPGWEGHVTLEISNTTPLPARVYANEGIAQFLFFKG
                     SKPCETSYADRSGKYMGQRGVTLPRL"
     gene            complement(14066..14230)
                     /locus_tag="PK513_02670"
     CDS             complement(14066..14230)
                     /locus_tag="PK513_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81389.1"
                     /translation="MVLYDMTIMKNTKQDKLDKRAAALRDNLKRRKTRAQSEKDEKND
                     ATDTEKNRAG"
     gene            complement(14688..15269)
                     /locus_tag="PK513_02675"
     CDS             complement(14688..15269)
                     /locus_tag="PK513_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HOO81390.1"
                     /translation="MSLRKTDRVFQTRNQELSNKKLAEIIAGALRSDFGDLYSSVKEI
                     DRATGIKFNTVKKWYEASTVPSLRHFLLLLECSSSLREFVLCYLFGEDILDDLALISS
                     VDQSRPVFENNTDFEPKNVTINDTIRLNERQKWFLLLLQNERKAAAKNIVEKWGVSFR
                     TARRDIQGLKEDGRIEYCGSNKSGRYSINRLGH"
     gene            15391..16962
                     /locus_tag="PK513_02680"
     CDS             15391..16962
                     /locus_tag="PK513_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO81391.1"
                     /translation="MYDERNQGNGGQAAGGKRAAMYLRMSDPRQECSTEIQEKIIRAY
                     AEKNDIEIVCTYADEGKSGVVATGRHSFQKLIADVENKEADLDFSLLLVYDVSRWGRF
                     QRTDESAHYEHICRLAGLEVHYVAEQFTNDGSPISELVKTVKRTMAAEYSRELSSKVF
                     NGQCHHVERGFRQGGPAGFGLRRMLIDENRNPKGQLEIGEQKSITTDRVILVPGPEEE
                     IEIVNWIYRMFVDEGWNETQIAADLNRREILTDFERPWTRASVHQILTNEKYIGTNVF
                     NRRSFKLKEKRINNPEEEWVKKENAFEAIIKPSYFYFAQGIIRERNRKYSNEEMLDKL
                     RILHERQGWLSGILIDENEDMPSSSAYASRFGSLIEAYKMIGYTPSRDIRYIEINRHI
                     RRLHPDIAEGVIHRIEDMGGHVYREVTSDLIVVNEEVKVSIVISRCHQTKAGRNRWKV
                     RLDTGLEPDITIVVRMDPANEHPLDYYILPAIDIEDPKLRLADNNGLALDCYRFDELE
                     DFFPLTRRITIQEAA"
     gene            16962..17852
                     /locus_tag="PK513_02685"
     CDS             16962..17852
                     /locus_tag="PK513_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO81392.1"
                     /translation="MKRKYDIQTIPINKIIVINSRDRSEKSFQAVKGNIESVGLKKPV
                     TVRPSDESDGYDLVCGEGRLKSFIALGQDSIPAIVRHDLSKEDAYVMSLVENMARRQH
                     TAMDLMKGIDLLKSQGYGVMDISRKTGLADSYVSGILTLLNKGEERLLSAVEKGHIPL
                     SIAIKIASSPGAEQKALQEAYEKEGLRGNRFVAAQRLIEKRRSLGKGMKGGGLRKNKE
                     GETLSARQMVQKFKTEMDRMRSLVEKANKTEATLMIAVESFYNLLQDENFKTLLRAES
                     LNTMPETLAEMIEQREASHV"
     gene            17845..18744
                     /locus_tag="PK513_02690"
     CDS             17845..18744
                     /locus_tag="PK513_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HOO81393.1"
                     /translation="MSDDVTSGFEGETIELDLDQIVPLKILSKSNLESRKFKQIISSI
                     KEVGIIEPLVVSRQSPKSKCYMLLDGHMRLEALKQMGETSAVCLISTDDEAFTYNKYI
                     SRKAPIQEHKMIQTMVKNGVSEAKIARTLNINVKSIIQKRSLLDGICPEAIDLLKDKM
                     VATIAFRYLKKMKPARQIECAMLMVDMNDFTVRYAQALLESSKDDELTDPIKPKVKRG
                     LSSEKRARMQEEMDKLAREYKLIKDDRGQKNLVLQFTKNYLARLLDNARVERFLRQHY
                     PDILEEFQKITDMTLKGGDPKSS"
     gene            18725..19129
                     /locus_tag="PK513_02695"
     CDS             18725..19129
                     /locus_tag="PK513_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81394.1"
                     /translation="MTRNQAEAGGAVLKGRGFTRSRHSRGHKETGILSRASREKRRDR
                     GRSRLVTARHGASTAAGMAANPHGACQGWRVICPAFQHINSDTPECERTGSWIASGLS
                     AGAHFFDIKNLGLSSKLLQIFKSYNNLILGFD"
BASE COUNT         4849 a         4298 c         4939 g         5130 t
ORIGIN      
        1 ggccacacgc ccataccata taaagaagtg gcggggaccg ggaaatcaca agtgaccttt
       61 gatcgggtga caatcgaaaa agcgctggat tatgccgctg aagatgcgga agtcacgctg
      121 cggctgtata agttgctcaa accgcgcctc gcactcgaga aaatggtaag cgtctatgag
      181 aatattgagc ggccgctgat cccggttatt gcgcagatgg agctggaggg aatcaaagtt
      241 gatcctgcga ttttaaaatc catgagcacg gatttcggca agcgcatagc cgatcttgag
      301 gaagaaattc acaaactcgc cggtacgccg tttaatatcg cttcgcccaa acaaatcggc
      361 gaaatcctgt ttgaccagat gggccttgat agtggcaaaa aaaccaagac cggccaacat
      421 tctacagatg taaaaacgct tgaagaactt gctttgcagg gccacgagat tgtgcaaaaa
      481 attctcgact ggcgtggtct ttcaaagctc aaatccacct atacggacgc gctgcaggat
      541 caggttgtcg cggaaacagg ccgtgtgcat acctcctatc atatgaccgg gacctcaacc
      601 gggcgtttgg cctcgtccga tccgaattta caaaatattc cgatccgcac cgaagacggg
      661 cgcaaaatac gtgaagcctt tatcgctgat acgggctgcg tcttgctctc ggttgattac
      721 tctcaggttg agttgcggct ggcggcggaa atggctggcg ttgaggcatt gcaacgagct
      781 tttaaggacg gggtggatat tcatacgttg actgcatccc aggttttcga tgttccactg
      841 gataatgtcg attctgaaac gcgcaggcgg gccaaggcgg tgaatttcgg cattatctat
      901 ggcatatctg gttgggggtt ggccaagcag ctgggctacg atcctgcaga tgcggctgat
      961 tttatcaagc gctatcttgc gcgctttcct gaaatcaagg attacatgga agagaaaaag
     1021 gatgaagcgc gtgccaacgg ctttgtcaaa acgctctatg gccgcaaatg ctttacgccc
     1081 aatattaatg cgaaaatccc ggcccagcgg gcaggctcag agcgtgcggc cgttaatgcg
     1141 cctttgcagg gcacggcagc ggatattatg aagatcgcga tggcaaaaat gcctgtggct
     1201 ttggagcgcg ctggattaaa agccaagatg ttgctgcagg tgcatgatga attgatcttc
     1261 gagctgccgg tggaagagtt ggaagcaacc tcaaagctgg tgcgtgagat catggaaaat
     1321 gcatggaaga ttatgggcgt ggagatttcc gtacctctca tcgccgaggc ggggtccggg
     1381 catagctggg ctgaggcgca ttagcgtgag cgcctaagat ttttttcgga taagttcttt
     1441 cagttctgcc atttcacggt gcagtgctat aatttcattc ccggagacaa tattgttttt
     1501 ccgtcgctct gggcctttat attcctcttt ttcatggatg atattcatgg catcaacgat
     1561 aatcccgata aaaaggttga ggactgcaaa gctcgtagtg ataataaagg gaataaagaa
     1621 cacccaggca taaggataga cttccatgac cgggcggacg atttcctcgc tccagccttc
     1681 tagcgtcata atctggaaca gtgtgtacat cgatgctcca atcgtgccga aaaacatggc
     1741 aaattcctca gtttgtccaa acagctttgt agatagcaca gacgctacat aaaaaataat
     1801 cagtaacacg gccatgatgg aagccatacc ggggatggca tgaaatagcg cggcgatcac
     1861 ccggcgcata gagggcacca cggataccaa ccgcaagacc cgcaagatcc gcaatgcccg
     1921 cagaatagcc aaaggtccgc tggcgggaat aagggcaatg ccaacaataa caaagtcaaa
     1981 aacattccag ccttgccgga aaaaggacag gcgataaaca aataacttga gcgcaagttc
     2041 aacaacaaag atcgtcagtg caatttcatc gaggtaaacc agcacatcgc tatagcgggt
     2101 cataacgctg gaatcggttt caagaccaag aattgcggca ttcacgataa tgatagcgat
     2161 aataaaattg gtaaaagtct tattttctat gaaacgtgct acgcgcccac gcagagcggt
     2221 aaactgaatg tctttgagat tggtcgggcg aaccattttt agtgtccttg tttatgtgtt
     2281 tgttcactcc taacaagttt tgcctctgtt ttaaattgaa aatttatgtt gttatgaagg
     2341 taagggaaga gttttttatc tatgaatgaa accatccaaa ttcttgtagg gcagagccaa
     2401 tatatccggg cctcttttgc aaagccaaag gagcacttaa tgcaaagtgt cgatcgcttg
     2461 ctggttatca tggttcacgg ctttccgggt gataaaaatg cttgtaataa tatttttgta
     2521 gaccttgaac atgttcttag tgaaaaacgt tatcacacac tgcgttttga ttttcgtggc
     2581 tgtggtgcca gcgacgggct ggccgaagat ttcacgcttt acagcgcctc cgaagatttc
     2641 gaaaccgtga taaaatgggc caaagacagg gggtatgagc gctttgtctt tatctgtgat
     2701 ggactcggcg cttcaatagc gctgatgaat gctccaaagg aagcaagatg ttacgtcttg
     2761 ttgtggccga tggttgactt gccgatgatt gcccagcatg tttttcaggc cgaagagatt
     2821 gaagaagatt ggaaaaaggc aggctacatt atgaaagagg atagccgtat cggcattcca
     2881 tttcttcaac agcttaaaaa agcaaaaatg accaagatcc ttaaagacct tggaaagcct
     2941 ctgctgatta tgcatggcgc gcaggacaag gtttcgccga ttgagcagct tgatatgttt
     3001 cgcgcttgcg caggccaccg ccgtgttgaa atcaccagct ttcaggatgg aacgcacggc
     3061 ctgccgcaag aaaaccaccg taaatcgatg ttttttcata tccgccagtt tatcgagaaa
     3121 tatacttgat ttttgcatga caatggcgct ataaacccgc atacgaatag atggcctggc
     3181 gagtacggct gcctcggcta tcagataaca accttatttt aaggagatat gaaatgccga
     3241 aaatgaagac caagtcgggc gctaagaagc gtttccgcgt tacgtcgagt ggcaaggtta
     3301 aggtcaaaca ggccaaaatg cgccacatgc aaatgaataa acccaagtcc atgaaacgta
     3361 aggctaaagg gcaaaccacg ttgtgtgatg ccgatgaacg cattgtgctg tgtaactggt
     3421 tgccttattc ccggaagaaa aagaaagcgt ctggcggcgt ttcagaaaag aaggagggct
     3481 agactatggc acgcgttaaa ggtggaccaa gagcgcacgc tcgtcacaga aaagttatca
     3541 aagcggctaa aggttactat ggccgccgga aaagttgttt ccgcacggcc gtccaagcgg
     3601 ttgaaaaagc cggtcagtac gcatatcgcg accgtcgcgc caagaaacgc gaattccgca
     3661 aattgtggat tcagcggatc aatgcagctg cgcgtttgca tgatatgacg tattcaacat
     3721 tcacgcatgg tttgaagctg gcgggaattg agctggatcg caaatctctg tccgagattg
     3781 ctatccacga tgagaaaggc tttgctgcga ttgccaagca agccaaggat gcgcttggta
     3841 aagctgaaaa agccgtggct taataaaacg tgttttccct gctaaagcag ggatctgata
     3901 aggggccata ccgcttagtg gtcaacgcct tgctttgtta tgtgtaaaat gatgagttta
     3961 atctgacgtg taaaagtcaa ggcttgatct gacgattatg aattatcggc tggtcggcgc
     4021 tttttgggta accttggtaa atatagtaat ttaaattact atactatccg gcctatcgtc
     4081 agattgctcc cgaacacatg gttcaggcca cgctcaccat attcttcgcg gataactgga
     4141 ttaattatct atactaagtt actaatgctg aatttttaca gacatgtaga atttttgttg
     4201 ctcgctgttt aatggattgt gatgcaccga tttaggtaat ccataattct ttcttttttg
     4261 ggcggctttg aaacaaagcc aacagcgcct ttttctattg cctgtagaac gttatccgcc
     4321 acactatcgg cactgaatac gataataaaa gcatctgtat cgacttcgat aattttatct
     4381 atcaattcaa gtccgctttt acccggcata tgaatatcca atagaagtac gtcgggatta
     4441 tgcctgagat attcactgac taccttgtca ccgctatcga cttctactac gtctccataa
     4501 aaacccagaa ttttttgcat ggctttgcgg acaaacatgt catcatcggc aaccatcaga
     4561 atgtttttct tccgctcttc gcgttctcga aacatattta tggattgccc atcctcttta
     4621 tccaacaagt cgatttgaat ttgttttaag cctgtgggac tcatttttct ggcgagcaca
     4681 cgacatgctt gctcaggcat cttttgttct aagtctgatt ttatattagc gtaattattg
     4741 ataacgccaa ggcgaaccag gcaaacgatc ttgcgttctt tgagagcata aacaatacct
     4801 tcatgctctt tgtaggcgat taatagcttt tcaagaattt cagcctgtga taaatgacaa
     4861 gcttttgtgg tcatgacatt cccaatatgg cattcattgg tgtgcggatt tcgtgcgaca
     4921 tattagccag aaaatcgctc ttggcacgac tggcctcttc ggcaacccgt tttgcttcct
     4981 ttaacgcgtt ttctttttct ttttgctctg agatatccaa agtcaatcca agaaaagtcg
     5041 tctcatgatc atttttgatt tctgtcacac tcagcttcat aggaaaggtt tgaccgcctt
     5101 ttctgaggcc ttcaacatct actcctgtgt ccaggatatg ggattcgcct gtctccagat
     5161 agttcttaag atactggtca tgctggctgt gatgcggttc cggcatgagc atgctgacat
     5221 ttttaccgat agcttcgtga gcctgataac tgaaaataaa ttccgcccac tggttgaacg
     5281 tctgaatgat gccattttta tctatcgtga tgacgccttg catcatatta ttcaaaatag
     5341 cgtcaagata cgttttttct ttttcgataa tggcatttaa gtgctcagcg tcttccttgg
     5401 acttccgcaa atctgcggtc tgttcctcaa cgcgtttttc aagattatca cggctgtttt
     5461 taaggtcttt tcgagctttc tcaatcgggt tggcaataaa gaaacgcgca taaagcaccg
     5521 ttgagagaaa aactaaaaac ccgataccta gaattatctg ttgtttgatc tccaaattct
     5581 ggcttttttg ttccagatat ttttggatgc ttttaacgga ttcgtcttgc gcttccagcg
     5641 ttatgcgcag ttgtgttgcg agttcatcat tctgcggcgt gttgataatt gttttgatgt
     5701 cagactgcaa tgttgtgacg gccagatgtt taagacgccc ccattctttt tgggctgtca
     5761 gcaatgctga ccgggtatcc ttgtcattaa aaggagttat ctcaagcgtt ttgtttccgt
     5821 caacgctcaa aataaccatc ccgccttcaa gcaatccttt ataattagcc tcaatgtgct
     5881 tttcgtcttg ttgtacaatc ttattatttt gcgcggcttc tttccagttg tgtgccgcat
     5941 gagtagccat aatgatcgag gtttggtgtg tgtagtgaac caacaacaga cgttgcaggc
     6001 ttgctgcgtt taacagggca ctatcgtttt ttttctcgct tacgctggcg ttggagaaaa
     6061 aataagatcc cacaagaaga accagaaaga gacttaacag cgatgagagt tgaagaaaaa
     6121 cggcattccc tgaatattga ttgtcttcca cttctgatgc cttgccttca gttggcttgt
     6181 acagactcag gattatggtg attgcaaaaa tagtgctgct ggttatggct acaatcatcg
     6241 ccaacgcgtt atagctttgg acagaatcgt agcggcagtc ggcataagga ataaaaacgg
     6301 aagcggccat gccggtgtag tgcattccgc aaattgccgc acccatgatt aatgccgccg
     6361 ctgcctgcca aaaggtcttt ccacgcccct catgctgtcc caacataaaa acgatgaaca
     6421 gggcggcacc tgatgcggtt acggcaatca aggcggacag ggaaaacagt cctggcgtat
     6481 aacgtaaatc ggcatccatt tccatagcgg ccatgcctgt ataatgcatg ccgcatattg
     6541 ctgttcccaa caataaagcg ctgataacta actgtagaat ctcaatcttt ccagtgcgga
     6601 taatctgcaa aacgccatag gcgatggcaa cggcaatgat catggataca aaagtcagga
     6661 atgggtcata ggtgtgcacc atatccatgt tataggccag catgccgata aaatgcatcg
     6721 accatattcc ggctccaaat gcaaaagcgc cgccaatatg aagatatgat ttttgttttt
     6781 ctgttttggc cttatgaatg tcggttgcca gccgcaagcc tgtgaaagat cccagcgatg
     6841 ctataacata agacagaata acaagtggaa tggcataggt gccataatat gcgtttggcg
     6901 gaatatcgcc aaagagaaag aagtgttcca gaaaagattg tggctcatgc agcatacctt
     6961 gtttatttta tctccgtaaa ttcagaaata acggccattt tgatgccatt taggcacatc
     7021 atcagaaaga ctgtcatctg caaccatgaa ttttatacaa aaacgtcttc ctggataggt
     7081 tactttttga gcttgcatgc atgtataaca caacccgtcc agcttgttct ggtactatac
     7141 actcttctat gggttgtggc aatgattggc ctaattaaat agggcagccc cttattaata
     7201 attattcaat cagcatgcct tgaaaacggt cagccagagg aggggttcgg cgtttgcggg
     7261 tgatttcgat gaggcccaga gcagtgattc cgtggatttg cacggtgcag ggatcctcgt
     7321 cgatggctgc ttcgagcgcg gcgaggagtt tgccttgatc tttgcggtcc tgggatttga
     7381 ggaagtcaat cacgatgatg ccgccgcaat tgcgcaggcg gatctggcgc gcggcttcgc
     7441 gggctgcttc aatattgacg tccagatggg aagcgcggct gccaccttta ttgacgtcga
     7501 tggcggtgag cgcggcggta ccctgaatga tgatgttacc tccgcctggt aacagggcgt
     7561 aggggtgaaa caggtcttcg atctggccga taatatcgcg atagtgaaac aaagcgagat
     7621 cttcagaggc gttatcaagt tcaatggggt cgattttggt aactagatca ggggcgaaga
     7681 ttgaacacca ttcctcgata tgtttaaaat gctccattgt aacaatttca atgcgatcaa
     7741 tggcttggct agcctgatca cttaaggtgc gctggatggc atcggggcca agggcaatta
     7801 agcccggctc tgggccttca aagtatttct ggatttcttc ccaagcgctt ttgaggattt
     7861 ttccttcacg gatcagaaca tcgctttggg tgtgagctgc ggcaaggcgt agaatgcaac
     7921 cctgaatgtc gaccatatta tccatcattt tatggagctg tttacgcagt tttttatcgc
     7981 ggatgcgttg ggaaatgcgg ttgcagtcca tattggcgca atagatcagg tagcggcccg
     8041 gcagagtgat gtccatactc atttgcggaa ttttgttttc ggaaggggta aaatcgtcct
     8101 gatcgtttga taagtaagcg gttttagcct gcagcgcaac catatcgccg ggttgaaaac
     8161 gtttggatat actttcgtcg ccacctttat gcactttgcc atcatcgtcg cgccaacgta
     8221 catccgcatt gtataaaatt ccggtattat cgccgtcgag atcgacaaag acagcatcaa
     8281 gcgctttatc gatggttttg acttttgccc aatagataga accccagcgt atttcttcgt
     8341 acggcgggtc gacttcaaga ccttcaaggc gtccgttttt aagggctgct gcccagagac
     8401 taccttccaa ctcttctatg agtatatcca ccgtgtttcc tcactgttgc tcttaggcgt
     8461 cagttttaaa gccagcgcct tctaatactc tcattgtatc ataaagcgat aggccgacca
     8521 cgtttgaata tgaaccgcgg atgaatttta tatagctttc ggcgtagcct tgaatagcat
     8581 aaccgcccgc cttgccccgc cactgcccaa agtggagata ttgatcaatt tctgaagggg
     8641 tgaggcgttt gaattggacc aaagtatcga tgaggcggtg gacggttttt cctgtgggcg
     8701 cgataagggc tatacctccg tagacgtgat ggcggcggcc tgagaggctt tccaggcaat
     8761 gacgggcata gggtttgtct tcggctttat cgaggatttt gcggcctgca ccaacgactg
     8821 tgtcagcagc gaggataaag ttaccgggat tctttttggc gatggcggcg gctttttcgc
     8881 gagcgaggcg ctgggccaga tcatgggggt gttcgccttt aagcggtgtt tcgtcaatat
     8941 cggccggaag gattttatcg gggatgatgc cgatttgttt aaggagatct agccgccgtg
     9001 gtgacgccga ggcaaggatg agtttatttt tcacggtaaa taatgcggcc tttggtaagg
     9061 tcgtaggggg tcatttcgac gatgacgaca tcgccttgca acacacggat gcggttttta
     9121 cgcattttac cagcggtgtg ggccaaaatg acatggtcgt tttccagcgt gacgcggaac
     9181 atcgcgttgg gcagaatctc ggatacgacc cctttgaatt ccattaaatc ttctttagcc
     9241 atcttgtcct gtgtctttgt tttttaagct gcattatcac gcaccttaaa attctatggt
     9301 gttgtttctt tagagaatat gggtatacac ccgctgcggg caagaatgca acggcttttc
     9361 tccacatggc gttaccacag tgccggcgca tgagcgctat gcagaacgca gatgagggtg
     9421 aagaggcgcc gggcggtgtc ttcgtccatt tctattttat cttcaaggcg ctcgattaac
     9481 aggccggcac cttcattatg caggccgcgg cggcccatat caatggcctc aatgcgtgat
     9541 ggcttgccgt cttgaacggc cgcatcatag ctgcgaacaa tcagaaaata gtctttaatc
     9601 agacggcgat agggcttaag ggacaggacc agcattggca atttattccc ttgcgcattt
     9661 tgtatgcgaa agacgaggcg gttttcttcg agcgtcaagc aaacatcata ggggccggcg
     9721 gtatctttaa aggggataaa gcggctggcg gcttcaagat cgcgcagggc aatatcgcgg
     9781 tcgcgggcaa tgagcgtgcc ttctggcgcg gctgtgatat tttcgatgcg gatggatttg
     9841 atcgctgttt ttgatggctt ggtcattttt gtacggcgga ctggaaagcg ttgacccagt
     9901 ggccaatacg ttcggcatcc gtttttacgg cgctgcggtt tacaacaagt ttcgatgata
     9961 tttccaggat tgtttcagtt tcggtgaggc cattggcttt gagcgttgag ccggtggaaa
    10021 ccagatcgac aatgcgacgg cagaggccaa gactgggagc gatttccatg gcaccgctca
    10081 gcttgatgca ttcggcctga ataccgctac gtgcatagtg acgggcggta aggctggggt
    10141 atttggtggc aatgcggata tggctttcat gcacggtatt ctgtttcttc gcatcggcat
    10201 cgggcatggc gacggacagg cggcatttgc caatacccaa atcaacgggg gcataaagtt
    10261 cgtcatagct gaattcctca attgcatcag aaccgacaac gccaatttgg gcaccgccat
    10321 aggcaacgaa agtggctaca tcgaaggcac gtacgcggat aatgtcgagg tccgcgtgat
    10381 tagtagaaaa gctcagcttt ctgctggagt cgctcatgaa atcatcctca gggacaatgt
    10441 cgcaggcgct gagcaaaggc ataagctctt cgaggatgcg gcccttgggc acagcgagaa
    10501 tgagtttcct atcagtagtc atggccattg gatagcctga atgatgcgcc gcggcaagat
    10561 tttttcttat ctgtaagagc ttgtgcttta cgttgtataa gtctttagga taaattgtta
    10621 tggttgctta ggattcatgg gggcattatg gatattttga ttgttgatga caatgctttt
    10681 attttagaga taatttcaaa aatgttggct ggtcaaggct ataagattca tcctaaaagt
    10741 aacgttgatg aagcaatttc gtatttagag ggcgggtcgg agtgtgattt ggtgattacc
    10801 gatattgtga tgccggaaaa ggatgggacg aagctcgcgt cttatgttaa atctaaaacg
    10861 ccgagcatgc cggttttggc gatgacaggc gggttggaaa atgcgctgga ggattatgtg
    10921 gttttggctg agatgtttgc tgatgaagtg atgaccaagc cgttccacaa aaatcagctt
    10981 atcgagacga ttgaacgtct ggtagcataa ggttggctta aatgctgtca aattaatcag
    11041 gcccttgggc agtacgagta tgggttggtt ttgtattgtg cgaatggagc gttgcgacaa
    11101 aagttaaagt gttcggctaa attcaagaat gttcaagggt ttctggtgtt gacgggtaaa
    11161 gcgcgcggtg ttggcttctc ttgcgatttt tgcaaaagct ttagcccatg aagagccatt
    11221 tctgaggttt ttgattacgc aatcactttc aatcctgttt tgttgtggat tgatgacgga
    11281 aaacgtatca ataagggaat atgcttttgt tgtattttca tttccatatg cgaagagaac
    11341 attacgaata tttttgggca ttctttctat ttctggacat gggcttacga atttggtgtt
    11401 gggtttagcc tgttgtgtgt gttgcgaaaa agcatggggg ggccgtgtag cgccggcctc
    11461 ttgtaaaatt tttctatatt ttattgccca tggctggttt gaacgatacg ctttaataac
    11521 cagatcactt tggcgcgtcc ttacatcttc aggtaagtct tgtagtttgt gtgccatttt
    11581 aaaagcttga agcttcttgc cgtcttggta ggccttaagg atatcaaaaa tttcagggac
    11641 atcagtttca taggtgcttt ggcagagatc ttgaatttct tgatcattga tcagagcgtt
    11701 tatggattca cgtgtttttc ggacatagca gcgtaaaatg ccgtttgttt gagtttgagg
    11761 atcttttgtt aataatcgga aaaaacctct atcggcttga tcgcgtgcgt gttcataagc
    11821 cagaacattt tctgcggttc tatcgcaaaa aagattctct aaagcattcg taaggctcaa
    11881 aaaaaacttt gcctatccag ctgacggcct taggcttttc attctcccca gtccagtttt
    11941 tatgctggtt tgtgatttgt ttttggagtg catcgattag agggatataa gactcgggaa
    12001 cttccatttc ccgaatttta taattttcat cttcttgctc tttgtcaaaa gataaattat
    12061 tgaatgctac tgcgccactc attatatttc catactcaaa aaaacactct cttttaccat
    12121 aatatttacg gtatgtcaag cttttgtcat atttgttttt ggaggacggt tttgatggtt
    12181 taagcgtgtt taatttgtgc accgcaggca ctgagttttt cgacgatatt ttcgtagccg
    12241 cgctcgaggt ggtagatgcg gttgatggtg gtttcacctt cggcgacgag gccggcaaga
    12301 acgagggcaa cgctggcgcg cagatcggtg gccatgactt cggcgccggt cagtttgggt
    12361 acaccgcgca cgatggcaga gtggccctgt acggtgatgt tggcacccat gcggttaagc
    12421 tctggtacat gcatgaaacg gttttcgaag atggtttcgg tgacgaggcc tgcgccttcg
    12481 gcgatggtca gcatggccat gaactgggct tgcaagtcag tggggaagcc cgggtgcggt
    12541 tcggtcatga tgtcgatggc cttgagcggg cactccggat cgcggctgac aatgatgtca
    12601 ctgccatcct gatcaatttc aaccccggtt tgggacagat gtccggcggc ggcgcttaaa
    12661 tgttcaaggt gggcattttg cagacgtaaa gatccgcctg tcatggcagc ggcaaccata
    12721 taagtgccgg tttcaatgcg gtcggggacg attgcgtggt gcgcaccatg catgtggctg
    12781 acgccttcga tgcggattgt gtccgtgcct aatccggtga tttttgcgcc cattttgatg
    12841 aggcattcgc ccagatcggt aatttcaggt tcgcgcgcag cgttggctaa taccgtttcg
    12901 ccgttggata aggttgcagc catgagcagg ttctcggttg cgccaacgga caccttgggg
    12961 aagaggattt tggtgccttg cagtccgcct tgcggcgctg ttgcgtggat gtagccttct
    13021 tccaatgtga tttttgcgcc gagttcttcc aacgccttga tatgcatatc gacgggacgg
    13081 gtgccgatgg cgcagccgcc gggcagggag accttggctt cgccgaaacg ggcgagcaac
    13141 gggccgagca cgaggattga tccgcgcatt ttgcggacaa tatcatacgg tgcggtttgg
    13201 gtatgtaccg gtccggcatt gatcgccatc agtggccctt cgcgtttaac ggcgcacccc
    13261 aaatattcga gcaattcggt ttgcgagttc atatcgcgca aggaattggg cgaattttcg
    13321 aaatagaggg attggtcggt gagcagggca gcgcacatca gcttgagtgc gcagttcttc
    13381 gccccgctga ttgggatgtc tccatgtaaa ggccgaccgc caacgacctt aattttatcg
    13441 agggaagatg aggctggagc ttttgcggat ggcgggcttt cgctaaagcc gcgggagcgt
    13501 aacgccgcgc tgtcccatat atttgccgga tcggtctgcg tagctggttt cacagggctt
    13561 gcttccttta aaaaacaaaa attgcgcaat gccctcattg gcgtaaacgc gggctggaag
    13621 gggtgtggtg ttggaaattt ccagcgtcac atgaccttcc cagcctggct ctagcggtgt
    13681 gacattcaca atgaggccgc aacgcgcata ggtgcttttc ccaaggcaga ttaccagcac
    13741 atcttttggg atgcggaaat attcgaccgt atgggtcagc acaaagctgt tgggagggat
    13801 tatgcaaaca ttggtctctc tgtcaacaaa actctgcgcc gagaagtctt tggggtcgac
    13861 gatggcgtta tcgacatttg tgaagatttt gaaatcggcg gcgcagcgcg catcatagcc
    13921 ataagaagac agtccgtagg aaatattgcc ttcgcgcttt tgtttttctt cgaagggttc
    13981 gatcatgcct tgggagcgcg cgagttcgcg aatctggtga tcggccagca ggccgggcgg
    14041 catgaggctc gcggaatcct gctggttatc ctgcccggtt tttttcagta tctgtggcat
    14101 cgtttttttc gtccttttca ctctgcgccc gtgttttgcg gcgtttgaga ttatcccgca
    14161 acgccgccgc acgcttgtcc agtttgtcct gcttggtgtt tttcatgatc gtcatatcat
    14221 acaaaaccat atggaaagcg aaagggaata atgattggag cacaaaattc tctggggaaa
    14281 gctctgatgc gcccccgctg taaaaaggga cgtgacaggc gcaaactttt attgtagttt
    14341 ttgcgccgtt acaaaagctg ctgctgtagc tcagtggtag agcgcaccct tggtaaggtg
    14401 acgccctcat cttttccggc gattcttagc cccgtttttt ctaaatttat caaatatttg
    14461 cgcggtcgga catttttcct gccgacacga tttttgtccg ggtaagcacc gggtaagcaa
    14521 ggggtggaaa aggcaatcta acaggggata atgagggaat ggggtaagca aataaggcat
    14581 atggtgcacc agagcgacca aaatgaaact ttttctccgc cacaagtgct tgtttttaca
    14641 attaaatcat cgcacagatt ttttcaatcc ccgtttttgg cttaaattta atgccctaat
    14701 ctattgatgc tgtagcgacc agatttattg gagccacaat attcgattct gccatcttcc
    14761 ttcaacccct gaatatctct tctggccgtt ctaaaactga caccccattt ttcaacgatg
    14821 ttttttgctg ctgcctttcg ttcattctgt agtaaaagaa ggaaccactt ctgtcgttca
    14881 ttgagtctta ttgtgtcatt tatagtgaca ttttttggct caaaatctgt gttattctca
    14941 aaaactggac ggctttgatc tacacttgaa atcaacgcca gatcatccag aatatcttct
    15001 ccgaacaaat aacacagcac aaattcccgt agtgatgaac tgcattcgag cagcaataaa
    15061 aaatgccgca gcgacggcac tgtgcttgcc tcataccatt ttttcacggt gttgaatttg
    15121 atgcctgtcg ccctgtcgat ctccttgacc gaagagtata gatcgccaaa atccgaacgc
    15181 agcgcgcccg caataatttc agccagtttc ttgttggata gttcctgatt tcgggtttga
    15241 aaaacccggt ccgttttacg caaagacata tgtatgctcc tttctttatg atgcaaaaag
    15301 aagcagttat gcaggcgatc caaggatttt accctgggtc attatcagca ttggcagcga
    15361 gttaacggct atgagacggg ctaattataa atgtacgacg agcgtaatca gggaaacggc
    15421 gggcaggccg cgggtggaaa gagggccgca atgtatttgc ggatgtcaga tccgaggcag
    15481 gaatgctcca ctgaaattca ggaaaaaatt atcagagcct atgccgagaa aaacgatatt
    15541 gagatcgttt gcacctatgc cgatgaaggc aaaagcggcg tggtcgcaac gggacgccat
    15601 tcttttcaaa aactgattgc cgatgtcgaa aacaaggaag ccgatctcga tttttctctt
    15661 ctgctggtct atgacgtttc gcgctgggga cggttccagc gcacggacga aagcgcgcat
    15721 tatgagcata tctgccgcct tgcggggctg gaagtccatt acgtagccga acaattcacc
    15781 aatgacggtt cccccatttc cgagctggtt aaaaccgtca agcgcaccat ggcggcggaa
    15841 tacagccgcg aattatcctc caaggtcttt aacggtcagt gccatcatgt cgaacgcgga
    15901 ttccggcagg gcggcccggc cggatttggt ctgcgacgta tgctgattga tgaaaaccgc
    15961 aaccccaaag gccaacttga aatcggtgaa caaaaaagca tcaccaccga ccgcgttatt
    16021 ctggtacctg ggccggaaga agagattgag attgtaaact ggatttacag gatgtttgtc
    16081 gatgagggct ggaatgaaac ccagatcgcc gccgatctga accgcaggga gattttaacg
    16141 gattttgaac ggccctggac acgggcaagc gtccatcaga ttttgaccaa cgagaaatat
    16201 atcggcacca atgttttcaa ccgccgatct ttcaaactca aagaaaagcg gatcaataac
    16261 cctgaagagg aatgggttaa aaaggaaaac gctttcgagg ccatcatcaa gcccagctat
    16321 ttttactttg ctcagggcat tatccgtgag cgcaaccgca aatattccaa tgaagagatg
    16381 ctggataaac tgcggatttt gcatgagcgg cagggctggc tctcgggaat attaatcgac
    16441 gaaaacgaag atatgccttc aagcagcgct tatgcctccc gttttggaag cctgattgag
    16501 gcgtataaaa tgatcggcta tacgccaagc cgcgacatac gttatatcga gatcaaccgc
    16561 catatccgta ggcttcaccc cgatattgcc gaaggtgtta ttcaccggat agaggatatg
    16621 ggcggtcatg tctaccgaga ggttacatcc gatctgatcg tggtcaatga agaggtcaag
    16681 gtctcgattg ttatttcccg ctgccaccaa accaaagccg gtcgtaacag atggaaggtt
    16741 cgtctggata cagggctgga gccggacatc acgattgtcg tacgcatgga tccggccaat
    16801 gaacacccgc tcgattatta catcctgccc gccatcgata ttgaagaccc caaattgcga
    16861 ctggccgata ataacgggct ggcgcttgat tgttaccgct ttgacgagct ggaagatttt
    16921 ttccctttaa cccgccgcat cacgatacag gaggctgcat aatgaaacga aaatacgaca
    16981 ttcagactat tcccattaat aaaatcatcg tcatcaattc acgcgaccgc agcgaaaaga
    17041 gcttccaggc cgtcaaaggc aatattgaaa gtgttgggct taaaaagccc gtaaccgtgc
    17101 gcccgtccga tgagagcgac ggctatgatc tggtttgcgg cgaaggccgt ttaaaatcct
    17161 ttattgctct gggacaggat agcattccgg ccattgtccg gcatgatctt tccaaagaag
    17221 acgcctatgt catgagtcta gtggagaata tggctcgcag gcagcatacg gccatggact
    17281 tgatgaaagg gatagatctg ctgaaaagcc agggatatgg cgtgatggat atttcccgca
    17341 aaacgggtct ggcggactct tatgtttccg gcattttgac attgctgaac aaaggtgagg
    17401 agcggctgct ttccgccgtt gaaaaaggcc acattccctt aagcatcgct ataaagatcg
    17461 cgtcttcccc aggcgcggag caaaaagctc tgcaggaagc ctatgagaaa gaaggtttga
    17521 ggggtaatcg ttttgtcgct gcgcagcgcc tcattgaaaa acgccgcagc cttggcaagg
    17581 gcatgaaagg cggcggcctg cgcaagaaca aggaaggcga aaccttgtct gcacggcaga
    17641 tggtgcagaa attcaaaacc gaaatggaca ggatgcgttc ccttgtcgaa aaggccaata
    17701 agaccgaagc cacgctgatg atcgctgtcg agtcctttta caatcttttg caggatgaga
    17761 atttcaaaac gctcctgcgc gctgaaagcc tgaacaccat gccggaaact ctggcagaaa
    17821 tgatcgagca gcgggaggcc agccatgtct gatgatgtca cgagcggctt tgaaggcgaa
    17881 accatcgaac ttgacctgga tcagatcgtg ccgcttaaga tactgagcaa atccaatctg
    17941 gaaagccgga aatttaagca aattatctcc tcaatcaaag aagtcggcat catcgaaccg
    18001 ctcgtagtta gcaggcaatc cccaaaatcc aaatgctata tgctgctgga tgggcatatg
    18061 cgccttgaag ccctgaaaca gatgggagag accagcgccg tttgtctgat ttcgaccgac
    18121 gatgaggcat ttacttataa caaatatatc agccgcaaag cccccattca ggaacacaag
    18181 atgatccaga cgatggtgaa aaacggcgta tcggaagcga agattgccag aacgctcaat
    18241 atcaacgtca aaagcatcat tcaaaagcgt agcttgctcg acggcatttg ccccgaagcc
    18301 atcgatctgc tcaaagacaa gatggtggcg acgatcgcgt tccggtattt gaagaaaatg
    18361 aaacccgccc gccagatcga atgcgccatg ctgatggtcg acatgaacga ttttaccgtg
    18421 cgttatgccc aggctttgct ggaaagctca aaagacgatg agcttacaga tcccatcaaa
    18481 cccaaagtga aaagaggctt gtcctcggaa aagcgcgcgc gcatgcagga ggaaatggac
    18541 aagctggcgc gtgaatacaa gctcatcaag gatgatcggg gacagaaaaa tctcgttctg
    18601 caattcacca aaaactatct ggcgcgcttg ctggataatg cccgtgtcga gcggtttttg
    18661 agacagcatt atcccgatat tctggaggaa tttcagaaaa tcaccgatat gacgctcaag
    18721 ggcggtgacc cgaaatcaag ctgaagcagg cggcgcggtt ttaaaaggtc gtggatttac
    18781 ccgcagccgt cattcaagag gccataagga gaccgggatc ctttcacgtg cgagccgtga
    18841 gaagcgccgg gaccgtggga gaagccgtct ggttacggcc agacatggag cgagcacggc
    18901 ggcaggaatg gcggcgaacc cgcacggagc ttgccaaggc tggcgcgtta tatgcccagc
    18961 cttccagcat atcaattcgg atacgccgga atgtgagcgc actggcagtt ggatagcttc
    19021 aggtctttct gctggtgcgc atttttttga tattaaaaat ttggggctgt cttcaaaact
    19081 tcttcaaatt tttaagagct ataataatct cattttgggt tttgattaaa cacactttcc
    19141 gcacaggccg tgaatttcca aattccagcg tgacggggtg aaggtatttt tagcggtgct
    19201 gtctttcagc ggctgg
//
LOCUS       DAOSWR010000020        19155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1812_length_19155_cov_5.639005, whole genome shotgun sequence.
ACCESSION   DAOSWR010000020 DAOSWR010000000
VERSION     DAOSWR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 19155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19155
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_19155_cov_5.639005"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK513_02700"
     CDS             <1..299
                     /locus_tag="PK513_02700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HOO81395.1"
                     /translation="YNPNSFLPHGTKKEGNPKDQPVWITAEDENPNNADMLILTHDQN
                     IPDNQEFALICYIFDGSNSEILSKARARWKTLKNADANLSYWQQSPQGCWEKKA"
     gene            complement(386..994)
                     /locus_tag="PK513_02705"
     CDS             complement(386..994)
                     /locus_tag="PK513_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HOO81396.1"
                     /translation="MENNKIFAAVLVAGIAAMLSGFIADRAVISEYPEQDAVTIEVAM
                     IGSANAAGADKADGPDPILGLIASADVAKGQKLSKVCAACHSFENGGPDKVGPNLYGI
                     VGASKASKSAFQYSGALSGLGGVWGYDELNNFLWKPKKYAPGTKMSYAGLKKPDDRAA
                     LIAWLRTQSGSPLALPSAAEIAAEAAPAESAAGEAAEAPAEH"
     gene            1128..1994
                     /locus_tag="PK513_02710"
     CDS             1128..1994
                     /locus_tag="PK513_02710"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOO81397.1"
                     /translation="MDLKNIKKIAFQGAKGAYSDLACRTVFPTLKTVPCDSFEDTFTA
                     VTGGKADLAMIPIDNTIAGRVADVHHLMPNGNLYIIGEHFQPIRHALLGIKGSKIEDL
                     KHVHSHVHAIPQCRKIIRRLNLKAHIHADTAGAAEDISNRNDPRHGAIASDLAAEIYN
                     LEILEHHIQDEHHNTTRFLILGPQPEIPDEDVPTITSLIFGVRNIPAALYKCLGGFAT
                     NNLSLSKLESYVDPNFQAARFFCDIEGHPDKNSFKLALEELGFYATDVKFLGAYPAHP
                     FRANNNKGTSIN"
     gene            2077..2415
                     /locus_tag="PK513_02715"
     CDS             2077..2415
                     /locus_tag="PK513_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81398.1"
                     /translation="MPTHAELASKLLIDAADFFLTLGEQNPPLKDQMEENATVFKQMG
                     ALLTQEPQGALNDTPHGELAGKLLIDAATFFTTLAEQNEPIKEQMLENANVYKQIGEL
                     VIKDPLGILD"
     gene            complement(2412..2780)
                     /locus_tag="PK513_02720"
     CDS             complement(2412..2780)
                     /locus_tag="PK513_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81399.1"
                     /translation="MPKGFAYTQAVQPFVSNGEPAYAVYVYHYPENQMEGQNDWEMRT
                     LTSDLKTALQDAQSLYKSSGYRRVEVKKRVHDSKSARTRDTIFKVFDCDSDKEIFKRR
                     CALAVVFGAAATLAYAFIAN"
     gene            3073..5580
                     /locus_tag="PK513_02725"
     CDS             3073..5580
                     /locus_tag="PK513_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOO81400.1"
                     /translation="MARTRTIRRAPKRRSTRKKQPFLLKFLPESLQAFIARRFVDGFA
                     SASTLSGLFILLALISYNPADPSWNTASSEGNDEISNWMGASGAWLADLFLQTLGIGA
                     ALFSLVLISWGIRSFNRQPLHPLWLRTIALLTGALFCAIAFSQMPSGDWIAHPYMGST
                     LGTLILSKAGLAGQEISPWLSPLVITTIATIIALPLAVYASATKMNEIGYLIQKSWRL
                     IVHTALFIIAAAYRFQQWLAHHNNPDYRPDPLAPFKAFPIKIPQKLATRTPPQISTPQ
                     TQNAAAINQVPTPPTPAPMAKPAEPIPVVTPQTSGTHNASPQQRFSLSDVENWELPNL
                     NLLQAVGTDNQSPQLNENALRRNAELLQNVLADFNIEGEITSIHPGPVVTLYELEPAP
                     GTKSSKVIGLADDIARSMSAISVRVAVVPGRNVIGIELPNKNRQIVYMQELLTTSLYE
                     KTSAGLPLALGKDISGQPIVADLAKMPHLLVAGTTGSGKSVAVNTMILSLLYRLPPEK
                     CRFIMIDPKMLELSVYDGIPHLLAPVVTEPGKAVVALKWTVAEMEERYRSMSKLGVRN
                     INGYNERVREAVKKGEKIMQKVQTGFTPDTGAPIFEDQEMDLSELPYIVVIVDEFADL
                     MLVAGKDVESAIQRLAQMARAAGIHLIMATQRPSVDVITGVIKANFPTRISFQVTSKV
                     DSRTILGEGGAEQLLGMGDMLYMAPGGRITRVHGPFVSDDDVECVVSDLKSKAEPSYI
                     DSITDSDFSDASEVMQAIFDDRGGTGEKIDELYDEAVALVAREGKASTSFVQRYLQIG
                     YNRAARIIEEMERQGVISKANATGKREVLISDFSDAG"
     gene            5723..6376
                     /locus_tag="PK513_02730"
     CDS             5723..6376
                     /locus_tag="PK513_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOO81401.1"
                     /translation="MRATKQDTQTLRILLLTALSAALIFLPYTGRAEKTESFNKDVKM
                     VESYLQNLSTARARFVQTTHDGTQLVGTFYLQRPGKLRFEYDEPIKDFVVADGFFIYF
                     YDAELGEQTNAPIGQTLADFFLRKDLSLEEDIKVESVRRKGGFLQVQLIQRSDPEAGS
                     LTLGFKEDPLTLSKWRVVDGQGLITEVELFYMKTDIKHPADLFVYADPARGTSEFND"
     gene            6387..6983
                     /gene="recR"
                     /locus_tag="PK513_02735"
     CDS             6387..6983
                     /gene="recR"
                     /locus_tag="PK513_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOO81402.1"
                     /translation="MQDGPLQHLIKTLSGLPGLGNRSARRIALHMLAHKNQTMLPLAD
                     LLKRTAEEIRTCSICGNLDMPDPCNICQDARRSKTQICVVAQVSDLWAIERTSAYKGQ
                     YHVLGGLLSALDGIGPNQLNLSSLIARAADPDITEVILALSATVDGQSTAHYIADHIN
                     RPQLEVTHLAHGVPVGGELDYLDDGTITTALRSRKKSV"
     gene            complement(7059..7889)
                     /locus_tag="PK513_02740"
     CDS             complement(7059..7889)
                     /locus_tag="PK513_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOO81403.1"
                     /translation="MKVLVVDQDDMSTQLIRSKLEAIGHQVVEEPMKNNAVERLAGDD
                     FDVVLFDPSPLTSPRPMILNARRSVKNYPYIVLISQSISQEEALKSGSNDFLKKPIDS
                     ALLLQKMDNAKRLIKLVQRIGDESEDFPSAGGVIAKSAFNQLFLSAMDRADRYGERTF
                     ILQISLNNYKELLEGSGSYAAEFAVAKLCQYLVLLRRQSDIIGQTAKNEYSLLLQRPI
                     YETEPTEAANRFAQELAALKDIDSGRAEPAEVLVSLIDVPTGTRLVEHVFKPNETVEN
                     "
     gene            8044..8131
                     /locus_tag="PK513_02745"
     tRNA            8044..8131
                     /locus_tag="PK513_02745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8078..8080,aa:Leu,seq:cag)
     gene            8236..8847
                     /locus_tag="PK513_02750"
     CDS             8236..8847
                     /locus_tag="PK513_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOO81404.1"
                     /translation="MSITLHKGDIPADLNFGNALAIDTETLGLAPHRDPLCLVQLSSG
                     DGKAHIVQLNRETYAAPNLKTLLADEKTLKIFHFARFDIAVIKKYLEVDCAPIYCTRT
                     ASRLARTYTDRHGLRDCCRELLGIELNKQQQSSDWGAEDLKDVQLKYAANDVLHLHEL
                     KERLDIMLKREDRIDLAQKCFDFLPIRAALDLAGWSNEDIFEH"
     gene            8899..9906
                     /locus_tag="PK513_02755"
     CDS             8899..9906
                     /locus_tag="PK513_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOO81405.1"
                     /translation="MNTPAPKNMQNQSDQDHARTVLQTEIEGLSALKDSLDKCFDETV
                     ETIHTMKQSGNHGRLIIAGIGKSGHVSRKIAATLASTGTPAYFVHPGEASHGDMGMIT
                     EHDVVLMLSNSGENTELSDLIHYTRRYNIPLIAMTSNPQSTLATHADICLKLPKAPEA
                     CPNGLAPTTSTTMMIALGDALAVSLLHRMGLTPEQYKIFHPGGKLGQKLMTVAELMIP
                     YDNLPLVDETAQMDKAILMLSEKNLGSVIIINEDGAVQGIITDGDLKRHMDANLLQKP
                     VTRIMSTSPKTIEKFSLAVEAVNIMTENPGHYISSLLVMENGKLAGMIRLQDCIQAGL
                     L"
     gene            9944..10663
                     /gene="lptC"
                     /locus_tag="PK513_02760"
     CDS             9944..10663
                     /gene="lptC"
                     /locus_tag="PK513_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOO81406.1"
                     /translation="MDNIQQKNNQNHAAEAHTGGPIKFSQLTRAESSRSHEGMRRYSA
                     FIRSLGIALPLIAIAITAIVFSWDVLKTNDIAAVPDIKTAKTIGKNELLNPKFDSVDN
                     KNQPFYITAKRALQDQDDALMLLDEPMADIALNNGNWLAVTARQGAYRQETQRLLLKD
                     NVTLFHDAGYKVTTQELDVDMNVGTAQTETSISGHGPLGTLNAKGMHADSKTQTLIFT
                     GPAKLVIYDTKTSGAGKVLKP"
     gene            10660..11502
                     /locus_tag="PK513_02765"
     CDS             10660..11502
                     /locus_tag="PK513_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ostA-like family protein"
                     /protein_id="HOO81407.1"
                     /translation="MKCKPFFILILLLTLSAANAQAQGLNTNSKDPLEITADGTLEWL
                     RGEKLFVATKNARAKQGDASVEGGRLSAYYREGKDGNNMQIWKVTADTNVILKNRDTI
                     IYGENAHYDLDQSLATMTGGNLKMISPDQTVTARDKFEYWAADGRVNAIGNAQVVRPK
                     IGGGQDTLRADKISAVLKNNAGSQQVLHSLEAIGNVVIITPTETVTGAYGIYKADTNI
                     AELTGGVKILRGPNTLEGQRAVVNLNTNISTIHGGDQTTEVPGKGQVRGVFYPGSQDK
                     TDTP"
     gene            11536..12165
                     /locus_tag="PK513_02770"
     CDS             11536..12165
                     /locus_tag="PK513_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOO81408.1"
                     /translation="MLWLVFASINLAATISPGPAFVITLRNAMAYDRKTGIFTAIGLG
                     LGVAIHAIFALAGIAVILAQSVFIFNVIKYIGATYLIYIGIKALQANKHNNKAVNAQK
                     QSNGKTNISTYKALQIGFLTNVLNPKALVFFPAFYMQFISSETSLTWLTAFGITSILT
                     EIIWFSLVTIVLTNPHIKARFMRIAHWIERICGGLLLALGVRLALSKIH"
     gene            12182..12961
                     /gene="lptB"
                     /locus_tag="PK513_02775"
     CDS             12182..12961
                     /gene="lptB"
                     /locus_tag="PK513_02775"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOO81409.1"
                     /translation="MIATPTKPPRRKEVPLKVVKEGLCVQHLSKAYKKRPVLRDVSFC
                     LKRGEVVALLGPNGAGKTTCFYITTGLIAPDGGYILLDGQDITALPMYRRARLGIGYL
                     PQESSIFRGLNVEENLRAVLESQNLNSEDQNLLLEELLSEFGIEHLRRTPSPALSGGE
                     RRRVEIARALAARPDFILLDEPLAGIDPIAVGEIRNLISHLKERGIGVLITDHNVRDC
                     LGIVDRAYILHDGKVLMEGNAEEIISDKNVRRVYLGEGFKL"
     gene            12991..14439
                     /gene="rpoN"
                     /locus_tag="PK513_02780"
     CDS             12991..14439
                     /gene="rpoN"
                     /locus_tag="PK513_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOO81410.1"
                     /translation="MSKLSQNLNLGQSQALVMTPQLQQAIKLLQLSNVELTEVVEEEL
                     ATNPLLEKDERTSEDNDSENAPEKHGEQDEIDEAFEKSNTNDDFDPGSSMADIGAGGN
                     SKFEDIENAFENRMSEDKTLRQHLIDQLHMTFEDNRDHMIGALLIDHLDESGYLREDP
                     EELAEKLGCSIERINTLIRELKGFDPTGIFAHDLADCMALQLSERGQLDGPMTTLLDH
                     LDMLANHDFKGLAEICGVNETYLKDMIEEIRSLNPKPAAEFDHLVVQTAIPDVIMKRL
                     PKNLGGGWRVELNTETLPRVLVNNEYYAEVARSATQKKDKEYLTTQLQNANWLVRAMD
                     QRAQTILKVAAEIIEQQDGFFNYGIEFLKPLKLSDIAAEIDMHESTVSRVTTNKYIGT
                     PRGIFELKYFFSTALVSESGAAHSAEAVKARIKTLTDEEDPKKILSDDKIVAILKDEG
                     IDLARRTVAKYREAMHIGSSVQRRKQKKHLAP"
     gene            14522..15121
                     /gene="raiA"
                     /locus_tag="PK513_02785"
     CDS             14522..15121
                     /gene="raiA"
                     /locus_tag="PK513_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOO81411.1"
                     /translation="MELNVHGKQIDVGDALRTHVEDKLEDLNQKYFNHATFATVTFSR
                     EGHGKASTKAHISVQLGKNIMVVADATDPDPYASFEAAAEKVGKQMRRYKRKLRDHHE
                     RLEQTPENEIFKARDYVLAAASEQDDEPEESLDQEPLVIAEMTKDIETMSVSEAVMRL
                     DLSGEPFLVFFNAKNGTLNIVHAREDGNIGWIDPENGEN"
     gene            15408..15878
                     /locus_tag="PK513_02790"
     CDS             15408..15878
                     /locus_tag="PK513_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO81412.1"
                     /translation="MNDYNRDLQFDVIMPNLRAISAKQVLMICAKEAEKHLGTPASML
                     FKLMVAKETQSNSGIGENVAIPDIQIRGPKKPFTMLATLAHPIDFNAADNQPVDIVGF
                     VLSPESDGPRHLRRLSRISRLLKNQDLHRKLHESHDQQTINTLLMDPDGWLMAA"
     gene            complement(16072..16302)
                     /locus_tag="PK513_02795"
     CDS             complement(16072..16302)
                     /locus_tag="PK513_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HOO81413.1"
                     /translation="MINNQRQTPEHLKKLSPQDFLSYGVQNIAYVREIHVDGRDGFAV
                     HAADGTPLSVMDSLEEALHIIRHNDLESAILH"
     gene            complement(16434..16931)
                     /locus_tag="PK513_02800"
     CDS             complement(16434..16931)
                     /locus_tag="PK513_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOO81414.1"
                     /translation="MSTGRLTLFDSPLFLGFDEIEKTFDRMRKSGAEGYPPYNIEQIG
                     ESGLRITLAVAGFTMETLDVEVESNQLVIRGRQPEEDEERVYLHRGIAGRQFQRSFVL
                     ADGIEVRGASLDNGLLHIDLERVIPESSARKIEIQGVKKNGVLPKAIDVEASDSEEKK
                     GKRGE"
     gene            complement(17050..17232)
                     /locus_tag="PK513_02805"
     CDS             complement(17050..17232)
                     /locus_tag="PK513_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOO81415.1"
                     /translation="MTDSMHVQSPEIISVPEACDGVLCDGGGGAFGHPKVWYSFEDRD
                     SVECSYCNRLFVKEGP"
     gene            17332..17721
                     /locus_tag="PK513_02810"
     CDS             17332..17721
                     /locus_tag="PK513_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81416.1"
                     /translation="MNAKTAIEFTDNKTAQMRPDQYIEITIDIARTLESWKNSLYSFE
                     WLENDGSIKPLNALSENEQAKRRAVEEALETGQPLEKPLLGIGILDNIEIGSGRAVLL
                     TLAAKGQTTMPVHIPKSCESDFKAFRA"
     gene            17746..18477
                     /locus_tag="PK513_02815"
     CDS             17746..18477
                     /locus_tag="PK513_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81417.1"
                     /translation="MKHARTKHHLDHQNESGNVLFYILIAVALIAALSFAVANSTRGN
                     IKQLNAERARLYATEIIEFANVIASAVSQIRLRGTDLSALCFDHASWGASDYNHAGCG
                     DDLNKIFHPSGAGLTWTQAPSEAMDSTATPDNLWHIYGDNEIENVGTTCGAAACADLI
                     LIVDELQLVVCQQLNDLLGVTDASTTPPTDSDFSETRYIGSFGYNETIGDEAGGEPLD
                     GKLTGCFQKTNAPAEYVFYKVLVAR"
     gene            complement(18578..>19155)
                     /locus_tag="PK513_02820"
     CDS             complement(18578..>19155)
                     /locus_tag="PK513_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO81418.1"
                     /translation="TVDGEKMSKSLGNFLLVHDLIETYPGEALRLTLLSAHYRQPLDW
                     SEDKVQQSRKQLDRFYTRLDEMAEMDAVETDVPQGVLDALCDDLNTPQAMAEMNALLK
                     KENSPELKGEILAAGNLLGILQQNPQEWLGFSGDVESGDNDSIEVLLQERAVAKVEKN
                     FARADEIRDQLTAMGIEILDTSEGTKWRKIS"
BASE COUNT         5527 a         5664 c         3834 g         4130 t
ORIGIN      
        1 cctataatcc gaacagcttc ctgcctcatg gtacaaaaaa agagggcaac ccaaaagacc
       61 agcccgtctg gatcaccgcc gaagatgaaa acccgaacaa tgccgatatg ctcatcctca
      121 cccatgacca gaacatccct gataaccaag aattcgcgct gatatgctat atctttgatg
      181 gaagcaattc cgaaatactc tctaaggccc gtgcacgctg gaaaacactt aaaaacgcag
      241 atgccaatct ttcttactgg caacaaagcc cgcaaggatg ctgggagaaa aaagcctaac
      301 tcgaaacagg ccaaccttca caaaaaaccg cctgcttaaa aaacaggcgg ctcttttaca
      361 attccatagt catttgagtt aaagcttaat gctcagcagg cgcctccgcc gcttccccgg
      421 cagcagattc ggctggtgcc gcctccgccg caatctccgc cgcactcggc aacgccaaag
      481 gactgccaga ctgcgtccgc aaccatgcaa tcaacgccgc ccggtcatcc ggctttttta
      541 aaccggcata actcatcttc gtacccggcg catatttctt aggcttccag agaaaattat
      601 tcaactcgtc ataaccccat actcccccca aaccagacaa agcgccggaa tattggaacg
      661 cgcttttgct cgcttttgaa gcgccgacaa taccatacaa gttcggcccg accttgtccg
      721 gaccgccatt ttcaaacgaa tgacacgccg cgcagacttt agaaagcttc tgccccttgg
      781 ccacatccgc gctcgcaatc aaccccaata tcggatcagg accatcagcc ttatccgctc
      841 cggctgcgtt ggctgaacca atcatggcaa cctcgatcgt cacagcatcc tgctcaggat
      901 attctgaaat aaccgccctg tccgcaataa acccgctaag cattgccgct attccagcga
      961 ccagaaccgc cgcaaatatc ttattgtttt ccatactttt atcgctttca aatattgagt
     1021 taaccctatg aaactatagc agcttgaaac cgcttgtcga cacagataaa cagtttgcgt
     1081 atagtcattc aaccaaaatt tttacaagta gaaagccgcc atccaaaatg gacctaaaaa
     1141 atatcaaaaa gatcgccttt caaggtgcca aaggcgccta ctccgatctc gcctgccgca
     1201 ccgtatttcc gacgctgaaa accgtaccat gcgactcatt cgaagatacg ttcaccgcag
     1261 taacaggggg aaaggccgat ctggccatga tcccgatcga caataccatc gcagggcgcg
     1321 tcgcagacgt acaccacctg atgcccaacg gaaaccttta cattatcggc gaacatttcc
     1381 aacccatccg ccacgcgcta ttaggaataa aagggtcaaa aattgaagat ttaaaacacg
     1441 tccacagcca cgtacacgcc attcctcaat gccgtaaaat tatccggcgt ctgaacctga
     1501 aagcccacat ccacgccgat acggcaggtg ctgcagaaga tatatcaaac cgcaacgatc
     1561 ccagacatgg cgccatagct tccgatttag ccgcagaaat ttacaatctc gaaatccttg
     1621 aacaccacat tcaagacgaa catcataata caacccgctt tttgatttta ggaccgcagc
     1681 cagaaattcc agacgaagat gtccccacaa tcacctcgct tattttcggt gtacgcaaca
     1741 ttcccgctgc gctctacaaa tgcctcggag gcttcgccac caacaatctc agcctgtcaa
     1801 aactcgaatc ctatgtcgat ccaaacttcc aggcagcccg ctttttctgt gacatcgaag
     1861 gccacccgga caaaaacagc ttcaaacttg ccctcgaaga actcggtttt tacgctacag
     1921 acgtcaagtt cctgggagcc tacccggccc acccgtttcg cgccaacaac aacaaaggca
     1981 ccagcataaa ttaaccaata tagacaatta aaccttgtga tgcctgggcc tggccgtgtt
     2041 agggtagcag aagtatagaa ttttaggagt taaacaatgc ccacccacgc cgaacttgcc
     2101 tcaaaattac tgatcgatgc cgcagatttt ttccttaccc ttggcgaaca aaacccccca
     2161 ctcaaggatc aaatggaaga aaacgccacc gtctttaagc aaatgggcgc actgctcaca
     2221 caagaaccac aaggcgcact caatgacacc ccgcacggcg aactggcagg aaaacttctg
     2281 attgatgcgg caacattttt tacaacactg gccgaacaaa acgaaccaat caaagaacaa
     2341 atgctggaaa atgccaatgt ttacaaacaa attggtgaat tggtcataaa agacccgctc
     2401 ggcattttgg attaattagc tataaaagca taagccagcg ttgccgctgc gccaaaaaca
     2461 accgccaacg cacatcgccg cttaaaaatt tctttatccg aatcgcaatc aaaaacctta
     2521 aaaatcgtat cgcgcgtccg cgcagattta gaatcatgta cgcgcttttt tacctcaacc
     2581 ctgcgataac cagaactttt atacaaagat tgagcatcct gtaacgccgt ctttaaatca
     2641 gatgtcaaag tccgcatttc ccaatcattt tgcccctcca tctggttttc gggataatga
     2701 taaacataaa cggcgtaagc tggctcaccg ttggaaacaa agggctgcac agcttgtgta
     2761 taagcaaaac cctttggcat caaaacctcc cgtaaactga gtgtgttaaa aacgaaattt
     2821 gaaaaagaaa cgcaggatca ataatctata cataacacca aaaagattaa cgaaaactta
     2881 atatcctctg aatcctgcgc aaaatctggc tttcatagaa ctcaaaacca aataaccggc
     2941 aaaacactgc aaaacttgtc tttagcgcta accttctgta aacttaacgc tcatcaaggc
     3001 tttggatgat tcttcagctt cagccccttc cagtaacaaa agcaaccaca ctacaacttt
     3061 attgaaaaca ttatggccag aacacgtaca atccgccgcg cgcctaaacg ccgctctacc
     3121 cgcaaaaaac aacctttttt actaaagttt ttgcctgaaa gcctccaggc tttcatcgcc
     3181 cgacgttttg ttgacggatt tgcctcagca tcgacactca gcggattgtt catcttactg
     3241 gccctgatca gctataaccc tgcagaccct tcatggaata ccgccagcag cgagggaaat
     3301 gatgaaatat caaactggat gggtgcttcc ggcgcatggc tcgccgacct gtttttacaa
     3361 acgctcggca tcggcgctgc cctgttctct cttgtcctga tttcatgggg tatccgcagc
     3421 tttaaccgcc agcccttaca cccgctttgg ctccgaacca tcgccctgct cacaggcgct
     3481 ctcttttgcg ccatcgcctt ttcacaaatg ccctccggcg actggatcgc ccacccctac
     3541 atgggctcaa ctctgggcac acttatcctc agcaaagccg gcctggccgg acaagaaatt
     3601 tctccctggc tcagcccgct cgtcatcaca acaatcgcca caataatcgc cctgcctttg
     3661 gctgtctatg ccagcgccac aaaaatgaat gagattggtt atctcattca aaaaagttgg
     3721 cgtcttatag tgcatacagc gctatttatt atcgctgcgg cctacagatt ccaacaatgg
     3781 cttgcccacc ataacaatcc agattaccgc cctgaccctc tcgccccgtt caaagctttt
     3841 ccgataaaaa taccgcaaaa actggcaaca cgtacgcccc cacaaatcag caccccacaa
     3901 acccagaatg ccgccgccat aaaccaggtg ccaacgccac caacacctgc tccaatggcc
     3961 aaacccgcag agccaattcc tgttgtcacg ccacagacaa gcggcacaca taacgccagc
     4021 ccacaacaac gtttcagcct ctcggatgtt gaaaactggg aactgccgaa ccttaatctc
     4081 ttacaggccg tcgggacaga caatcaatcc ccgcagctca atgaaaacgc cctgcggcgt
     4141 aatgcggagc tactgcaaaa cgtactggcg gatttcaata tcgaaggcga aattacctcc
     4201 atccaccccg gccctgttgt cacgctttac gaactcgaac ccgcaccggg tacaaaatcc
     4261 tccaaagtca ttggactggc cgatgacatc gcccgctcta tgtccgccat ctctgtacgc
     4321 gtcgctgtcg tgccaggccg caacgtcatc ggcatcgaac tacccaacaa aaaccgccaa
     4381 atcgtttata tgcaggaact cctgaccaca tcactctatg aaaaaaccag cgcaggcctg
     4441 ccgctggctc ttggtaaaga cattagcggc cagcctatcg tcgccgacct cgccaaaatg
     4501 cctcacctcc tggttgccgg gaccactggc tcggggaaat ccgtggccgt gaataccatg
     4561 atcctctcac tgctctaccg cctgccaccg gaaaaatgcc gcttcatcat gatcgacccc
     4621 aaaatgctcg aactctccgt ctatgacggc atcccgcact tgctcgcccc cgttgtaacc
     4681 gaacccggca aagccgtcgt cgccctcaaa tggacggtgg cggagatgga agaacgctac
     4741 cgctctatgt ctaagctcgg cgtacgcaat atcaacggct ataatgaacg cgtccgcgaa
     4801 gccgtcaaaa aaggcgaaaa aatcatgcaa aaagtccaga ccggtttcac gcccgatacc
     4861 ggcgcaccaa tcttcgaaga tcaggaaatg gacctttccg aactgcccta tatcgtcgtc
     4921 atcgtcgacg aatttgccga cctcatgctc gtcgcaggca aagacgttga aagcgccatc
     4981 cagcgcctcg cccagatggc ccgcgcagcc ggaatccatt tgatcatggc cacgcagcgc
     5041 ccttcagtcg atgtcatcac cggcgtcatc aaagccaact tcccgacccg tatttccttt
     5101 caggtcacat caaaagtcga ttcacgaact attctgggcg aaggcggcgc cgaacaactt
     5161 ctgggtatgg gcgacatgct ctatatggcc ccgggcggac gcatcacacg cgtacacggc
     5221 ccgttcgtca gcgatgacga cgtcgaatgc gtagtcagcg acctcaaatc caaagccgag
     5281 ccctcttaca tcgattccat cactgacagc gattttagcg atgccagcga agtcatgcag
     5341 gccatcttcg atgatcgggg tgggacaggc gaaaaaatag atgagcttta tgatgaagcc
     5401 gtcgccctcg tcgcacgcga aggcaaagcc tccaccagct tcgttcaacg ttatttgcaa
     5461 atcggctata accgcgccgc acgcattatc gaggaaatgg aacgccaggg tgtgatctca
     5521 aaagccaacg ccaccggcaa acgcgaagtc ttgatcagcg atttctccga cgcgggttga
     5581 tcgaataaaa cacggcaaat accacaaaat accgcccagt ctgtatttta aaatatctcg
     5641 cagacacgca aacatcaaat cctgaccacc aaacaaatct tttaccaaag ggtttgcaaa
     5701 gccgcctgcg tatactatgt gtatgagagc aacaaaacag gatacgcaaa cattgagaat
     5761 tcttcttcta acagccctca gcgctgcgct aatttttcta ccttacacag gtcgggcgga
     5821 aaaaacagaa agcttcaaca aagatgtgaa gatggtcgaa tcctacctgc agaatttatc
     5881 gaccgcgcgt gcccgcttcg tacaaaccac ccatgacggc acacagcttg ttggaacttt
     5941 ttacctccag cgtcccggca agctccgctt tgaatatgac gaacccatca aggatttcgt
     6001 cgttgccgac ggttttttta tctatttcta tgatgcggaa ttgggagaac aaaccaacgc
     6061 gcccatcggc cagacattag ccgatttttt cctgcgcaaa gatttgtcac tggaggaaga
     6121 cattaaggtc gaaagcgtcc ggcgtaaagg cggcttttta caggtccagc tcatccagcg
     6181 ctccgaccct gaagcaggct cacttacact gggctttaaa gaagacccgc taacactcag
     6241 taaatggcga gtcgtagacg gacaaggcct catcaccgaa gtagagctct tctatatgaa
     6301 aaccgatatt aagcacccgg cagatctgtt tgtttacgct gatccagcgc gcggcacctc
     6361 cgaatttaat gattaaagct tagcaaatgc aggacggccc tttacaacat cttatcaaaa
     6421 cactctccgg cctgcccggc ctaggtaatc gctcagcccg gcgcatcgcc ctgcatatgc
     6481 tcgcccataa aaaccaaacc atgctgccac tggccgattt actgaaacgt acggccgaag
     6541 aaatccgcac ttgcagcatt tgcggcaatc tcgatatgcc cgatccttgc aatatttgcc
     6601 aggatgcccg gcgctctaaa acgcaaattt gcgtggttgc acaagtcagt gacctctggg
     6661 caatcgaacg aacctcggcc tataaaggac aatatcatgt tctgggcgga ttactctccg
     6721 cacttgatgg catcggcccg aatcaactca acctgagcag tctaatcgcc cgcgcagccg
     6781 atcccgacat tacagaagtc atcctcgccc tcagcgcgac cgtggacggc caaagcacag
     6841 cgcattacat cgccgatcac atcaaccgcc cgcagttgga agtcacccac ctcgcccacg
     6901 gcgtaccggt tggcggtgag cttgattatc tcgacgacgg caccatcacc accgccctgc
     6961 gttcacgcaa aaaaagcgtc tgattacacc agtcataata gaacataccg accggtcggt
     7021 atgttctggc ttaactctgc aattttaaag ggttccgctt aattttcaac ggtttcatta
     7081 ggcttgaaaa catgctcaac aagccgcgta ccagtaggaa catcgatcaa ggaaaccaaa
     7141 acttcagcag gttcagcacg tcccgaatca atgtctttaa gcgcagcaag ctcctgcgca
     7201 aaacgattgg cggcctcggt tggctccgtc tcataaatcg gacgttgcaa caacaaacta
     7261 tattcattct tggccgtttg cccgataatg tcactctgtc ggcgcaaaag caccaaatac
     7321 tgacacagct tcgccacagc aaactcagcc gcatatgaac cgctcccttc aagcaactcc
     7381 ttataattat ttaaagaaat ctgtaaaata aacgtccgtt caccataacg atccgcacga
     7441 tccatcgctg aaagaaacag ctgattaaag gccgatttag caatcacacc accggcactg
     7501 ggaaaatctt cagattcatc gccaatacgc tggactaatt taatcaaacg cttggcatta
     7561 tccatctttt gcaaaagaag cgccgaatca atcggttttt taagaaaatc attcgaaccg
     7621 ctcttaagcg cttcttcttg agaaatactc tgcgatatta aaacgatata agggtaattt
     7681 ttaacactgc gccgcgcatt taaaatcata ggccgtggac ttgtcagagg actgggatca
     7741 aacaaaacca catcaaaatc atctcctgca agcctttcaa ccgcattatt tttcatcggt
     7801 tcctcgacaa cctgatgccc aattgcctca agcttggaac gaataagttg cgtagacata
     7861 tcatcctgat caacgactaa aactttcata gtgctgttct tcccctatat cgaacaaatc
     7921 tctcccatta tgatcgttta aaattaataa aatgcaaagg cctaatttat agaactttct
     7981 caaatcccgt atttgacaac cttgtctgag tacgatagac aatcactcac tttgaatgaa
     8041 tgtgcccagg tggcggaatt ggtagacgcg ctagcttcag gtgctagttc cccttgtggg
     8101 agtgaaggtt caagtccttt cctgggcacc attgattttc ttatataaaa agacgactat
     8161 taagaaaatt gaaggctgaa acagcatatg aaaagaggct gcagcgtaac tttcatacaa
     8221 taaaaaggcg tataaatgag tattaccctc cataaaggcg acatcccggc agatcttaat
     8281 tttggcaatg ctctggccat agacacggaa actctgggct tagccccaca tcgtgaccca
     8341 ttatgccttg tacaactctc cagcggtgac ggcaaagccc acatcgttca gcttaaccgc
     8401 gaaacctatg ctgctccaaa cctcaaaacg ctgttagccg acgaaaaaac gttgaaaatc
     8461 tttcattttg cgcgcttcga cattgccgta attaaaaaat atcttgaggt cgattgcgcc
     8521 ccgatttatt gcacacgtac agcttcacga ctcgcgcgca cctataccga ccgccacggc
     8581 ctgcgcgact gttgccggga attgctcggc attgaactca ataaacaaca acaatcctca
     8641 gattggggag cagaagacct caaagacgta cagctcaaat acgcggccaa tgacgttctc
     8701 cacctccacg aactcaaaga acgtcttgac atcatgctaa agcgcgaaga tcgcatcgat
     8761 cttgcacaaa aatgctttga cttcctgccc atacgtgccg cgctagacct ggctggatgg
     8821 agtaatgaag atattttcga acactaaaag ctgccacgct caaacaatat aacgcgcgcg
     8881 aacattgaaa gacgagccat gaatacaccg gcccccaaaa acatgcaaaa tcaaagcgat
     8941 caagaccacg cccgcactgt tctgcaaaca gagatagaag gcctcagtgc gctcaaagat
     9001 tctctcgaca aatgcttcga tgaaactgtt gaaaccatcc atacaatgaa acaatcaggc
     9061 aatcacggtc gcctcatcat tgcaggcatc ggcaaaagtg gccatgttag ccgtaaaatc
     9121 gctgccaccc tcgcctccac cggtacgcct gcttattttg ttcatcccgg cgaagccagc
     9181 cacggggata tgggcatgat aaccgagcat gatgtggtcc tcatgctctc caattccggc
     9241 gaaaatactg agctatccga cctcattcac tacacgcggc gctataatat cccgttgatc
     9301 gctatgacct ccaacccgca aagcaccttg gccactcacg ccgatatatg cttaaaacta
     9361 cccaaggccc ccgaagcctg tcccaacggc ctggccccga ccacctcgac caccatgatg
     9421 atcgcactcg gcgatgccct cgccgtttcg ctgctccatc gcatggggct gacgcccgaa
     9481 caatataaaa tcttccatcc gggcggaaaa ttgggccaaa aactcatgac cgtcgccgaa
     9541 ctgatgatcc cctatgacaa cctgcccctt gtcgacgaaa ccgcgcagat ggacaaggca
     9601 attttaatgc tgagtgagaa aaaccttgga tcggtaatta taataaacga agacggcgcc
     9661 gtacaaggca tcatcaccga cggtgatctt aaacggcaca tggatgcaaa tttgctgcaa
     9721 aaacccgtga caagaatcat gagcaccagc cccaaaacca ttgaaaaatt ctcactggcc
     9781 gtcgaagccg tcaatattat gacagaaaat cccggacact atatctccag cctgctggtc
     9841 atggaaaacg gaaaactcgc cggaatgatc cgcctacaag attgtattca agccggactt
     9901 ctgtaatata ccccattcaa tagggcgatt aaaggcaaac aggatggaca acatccaaca
     9961 aaaaaataat cagaatcatg cggctgaggc acatacaggc ggccctataa aattcagcca
    10021 gctcacccgc gccgaaagct cccgtagcca cgagggaatg cgacgctact ctgccttcat
    10081 ccgctctctg ggcatcgcac tccccctcat cgccatcgca atcacagcca ttgtcttttc
    10141 atgggacgtc ctgaaaacga acgacatcgc cgccgtgccc gatattaaaa ccgctaaaac
    10201 cattggcaaa aacgaattgc ttaaccccaa attcgacagc gtcgataata aaaaccagcc
    10261 cttttacatc accgccaaac gtgccctgca agatcaggac gatgcgctga tgctgctcga
    10321 tgaacccatg gccgacatcg cactcaataa cggcaactgg ctggccgtca ccgcgcgtca
    10381 aggtgcctat cgccaggaaa cccagcgcct cctgctcaaa gacaacgtca cactctttca
    10441 tgatgcaggc tataaagtca ccacccagga acttgacgtc gacatgaacg tcggcaccgc
    10501 tcaaaccgaa acatccattt cagggcacgg ccctctaggc acactaaacg ccaaaggcat
    10561 gcacgctgat tccaaaacac aaacgctaat atttacaggc ccggcaaagc ttgtcattta
    10621 tgacaccaaa acctcaggcg cagggaaagt actcaaacca tgaaatgcaa accgtttttc
    10681 attttaatcc ttttactcac gctaagcgca gcaaacgcac aggctcaagg cctaaacaca
    10741 aattcaaaag accctctcga gatcaccgcc gatgggaccc tcgaatggct gcgcggagaa
    10801 aaactgtttg tcgccacaaa aaatgcccgc gcgaagcaag gagacgcttc tgtcgaagga
    10861 ggacgtctta gcgcatatta ccgcgaaggc aaagatggca ataacatgca aatctggaaa
    10921 gtcaccgccg acacaaacgt cattttgaaa aaccgtgaca ccataatata cggcgaaaat
    10981 gcacactatg atttagacca gagcctcgcc accatgaccg gaggcaacct caaaatgatt
    11041 tcacccgatc aaaccgttac cgcgcgtgat aaattcgaat attgggccgc cgacggccgc
    11101 gtcaacgcca tcggcaacgc gcaagtcgtg cgccccaaaa tcggcggcgg ccaggacacc
    11161 ctgcgtgccg acaaaatctc cgccgtgctt aagaacaatg ccgggagtca gcaagtcctc
    11221 cactctttag aagccatcgg caatgtcgtc atcataacgc caaccgaaac cgtcaccggc
    11281 gcttacggta tctataaagc cgataccaat attgccgaac tgaccggcgg cgttaaaatt
    11341 ctacgcggac ccaatacgct cgaaggccag cgcgccgtag tcaatctgaa caccaatatc
    11401 agcaccatcc acggcggaga tcaaaccacc gaagtgcccg gcaaaggcca ggtacgcggc
    11461 gttttctacc ccggatcgca agataaaact gataccccct agtctttttg ttaggatatg
    11521 aacatggaat ttataatgct ttggctcgtt ttcgcttcaa tcaatctcgc cgcaacaata
    11581 tcacccggcc cggccttcgt catcacattg cgcaatgcca tggcctatga tcgcaaaacc
    11641 ggaatcttca ccgcaatcgg cctcggcctt ggtgttgcca tccatgccat tttcgccctg
    11701 gccggcatcg ccgtaattct ggcacagtcc gtattcattt ttaatgtcat aaaatatata
    11761 ggcgcaactt acctgatcta tatcggcatc aaagccctcc aagccaataa acacaacaac
    11821 aaagccgtaa acgcccaaaa acaaagcaat ggcaagacca atatttcaac ctacaaagcc
    11881 ttgcaaatcg gcttcctaac caatgtacta aatcccaaag ctttggtctt tttccctgcc
    11941 ttttacatgc agtttatctc gtccgaaaca agcctgacat ggctaaccgc ctttggtata
    12001 acatcaatac taaccgaaat catttggttc agcctcgtca caatcgtact caccaacccc
    12061 cacatcaaag cccgcttcat gcgcatcgcc cactggattg agcgcatctg cggtggactt
    12121 ctcttagcgc ttggtgtacg tttagcctta agtaaaatac attaaagacc aagctaaccg
    12181 catgatcgcc acacccacaa aaccaccacg ccgcaaagaa gtcccactga aagtggtcaa
    12241 ggaaggtctg tgcgtacagc acctctccaa agcctataaa aaacgccctg tcctgcgcga
    12301 cgtttcattc tgccttaagc gcggcgaagt cgtcgccctg ctcggcccca acggcgctgg
    12361 taaaaccacg tgtttctaca taaccaccgg actgatagcc ccggacggcg gctatatcct
    12421 gttagatggc caagacatca ccgccctgcc gatgtaccgc cgcgcccgcc taggcatcgg
    12481 ctacctgccg caagaatcct cgattttccg cggcctcaat gttgaggaaa atctccgcgc
    12541 tgttttggaa tctcaaaatc tcaactccga agaccagaac ctcctgctcg aagaactctt
    12601 aagcgaattc ggcattgaac atttgcggcg tacaccctcc ccggccttgt ccggcggcga
    12661 acgccgccgc gtcgaaatcg cccgcgctct ggccgcccgt ccagacttca tcctgcttga
    12721 tgaaccgctg gctggaatag accccatcgc cgtcggagaa atccgaaatc tcatctcaca
    12781 cctgaaagaa cgcggcattg gcgtcctgat taccgaccac aatgtccgcg actgcctggg
    12841 catcgttgac cgcgcctata tcctgcacga tggaaaagtc cttatggaag gaaacgccga
    12901 agaaattatc agcgacaaaa acgtccgccg cgtctatctc ggtgaaggct ttaaactata
    12961 attagaccac aatcgtggta taatgaccgc atgagtaagc tctcccaaaa cctcaatctc
    13021 ggccaatcgc aggcactcgt catgaccccg cagctgcagc aggccatcaa gcttctgcaa
    13081 ctcagcaatg ttgagctgac cgaggtcgtc gaagaagaac tggccaccaa cccgctgctc
    13141 gaaaaagatg aacgcacatc tgaagacaac gacagtgaaa acgcgccgga aaaacacggc
    13201 gaacaagacg aaatcgacga agcctttgaa aaatcaaaca ccaatgatga ttttgacccc
    13261 ggctcctcca tggccgacat cggcgcaggc ggtaattcaa aatttgagga cattgaaaat
    13321 gcctttgaaa accgtatgtc cgaagataaa accctgcgcc aacacttaat cgaccagctc
    13381 cacatgacct ttgaagataa ccgcgaccac atgatcggtg cgctcctcat cgaccatctc
    13441 gacgaaagcg gctacctgcg cgaagacccc gaagaactcg ctgaaaaact cggctgctcg
    13501 atcgaacgta taaacaccct catccgtgaa ctcaaaggtt ttgaccccac tggcatcttc
    13561 gcccatgatc tcgccgattg catggcgctt cagctttccg aacgcggcca gcttgatggc
    13621 ccaatgacca cgctccttga ccatctcgac atgctcgcaa atcatgattt taaaggtctg
    13681 gcagaaattt gcggtgtcaa cgaaacctac ctcaaagaca tgattgaaga aatccgcagc
    13741 cttaacccca aaccggccgc agaattcgac cacctcgtcg ttcagaccgc cattcccgac
    13801 gtaatcatga agcgcctgcc caaaaacctc ggcggcggct ggcgcgtcga actcaacacg
    13861 gaaacccttc cccgcgtatt ggtcaataac gaatactacg ccgaagtcgc acggtccgcg
    13921 acccagaaaa aggataagga atatctaacc acccagctcc aaaacgccaa ctggctggtg
    13981 cgcgcaatgg atcaacgcgc ccaaaccatt ctcaaggtcg cagccgaaat catcgaacag
    14041 caagacggct ttttcaatta cggtatcgaa tttctcaaac ccctcaaact ctctgatata
    14101 gccgccgaaa tcgacatgca cgaaagcacg gtttcccgcg tcaccaccaa caaatatatc
    14161 ggtacgccgc gcggcatttt cgaactgaaa tatttcttct ccaccgccct ggtttctgaa
    14221 agcggcgcgg cccacagcgc agaggctgtc aaggcccgta ttaaaaccct caccgatgaa
    14281 gaagacccga agaaaatcct ttctgacgat aaaatcgtcg caatccttaa agatgaaggc
    14341 atagacctag cccgaagaac tgtggccaaa taccgcgagg ccatgcatat cggctcatcc
    14401 gtccagcgcc gcaaacaaaa gaaacattta gcgccttaaa cgccgaaacg cgttgcattc
    14461 cgcccgccga aggtgctaaa ttgtgaccag aaaaaacaca tacacataag aggaagacca
    14521 aatggaactc aatgttcatg gcaaacaaat tgacgtcggt gatgctctgc gcacccatgt
    14581 tgaggacaag ctcgaagacc tcaaccagaa atattttaat cacgcaacct tcgcaactgt
    14641 aaccttctca agagaaggcc acggcaaagc aagtacaaaa gcgcatattt cggttcagct
    14701 cggcaaaaac attatggtcg tcgccgatgc aaccgacccg gacccttacg catctttcga
    14761 agccgcagct gaaaaagtcg gcaaacaaat gcgtcgctat aaacgcaaat tacgtgacca
    14821 tcatgaacgc ctcgaacaaa cgcctgaaaa cgaaatcttt aaggcccgtg actacgttct
    14881 ggcagcagcc tccgaacagg atgacgagcc cgaggaaagt ctcgaccaag agcctttggt
    14941 cattgccgaa atgaccaaag acatcgaaac aatgagtgtt tccgaagccg tcatgcgtct
    15001 ggatctgtcc ggcgaaccat tcttggtatt ctttaacgcc aaaaatggca ctctgaacat
    15061 tgtccacgcc cgcgaagatg gcaatatcgg ctggatcgat cctgaaaacg gtgaaaattg
    15121 atcaaaaacc tgtcagaaaa aacttttttc actatccaca aatttaaaaa tttttctttt
    15181 cgggggccca aaaagccccc gaatactttt ttcatagaca ttaaagcatc aaaaattaac
    15241 tccagcaaaa atcctcgcaa aaccgcgccc atattcaaaa aatctaatga tgcatcgcag
    15301 catatttttc cctggacccg cccataatat aacagtactg acatgctcaa caacactctg
    15361 ggttgtttga aaaatcaaac aaatctaaac gttggggaaa taaaaatatg aacgactata
    15421 accgtgattt gcaatttgat gtcataatgc cgaacttacg cgccatcagt gcaaaacaag
    15481 tccttatgat atgcgcaaaa gaagctgaaa aacatctcgg cacccccgca tcaatgctat
    15541 tcaaactcat ggtcgccaaa gagacccaat caaactctgg aatcggtgaa aacgtcgcca
    15601 ttcccgacat ccaaatccgc ggcccgaaaa aaccgttcac catgctggca acgcttgctc
    15661 accctattga ctttaatgcc gctgataatc agcctgttga tatagttggt tttgtattgt
    15721 ctccggaaag tgatggtcct cgccaccttc ggcgactctc gcgcatctcc agattgctaa
    15781 aaaatcaaga cctgcaccgc aaacttcacg aatctcacga ccaacaaacc ataaacacct
    15841 tgctcatgga ccccgacgga tggttaatgg cggcataaga tacagcctct cccaaaagaa
    15901 accccgcatt caatgcgggg gccctataat ctctccctct tccaccgtca tcgcaagcgt
    15961 agcaaagcaa tccacaaaac acctccgcct ctccgcacct ccgcgtaaat ttcaaaaaac
    16021 aaaaccgcct ttaataacaa ggcggttttt tacattccaa actactaatg cttaatgcaa
    16081 gattgcggat tctaaatcat tatgacgaat gatatgcagc gcttcttcca gactatccat
    16141 gacagacagc ggcgtcccat cagccgcatg caccgcaaaa ccatcacggc catcaacatg
    16201 aatttcgcgt acatacgcaa tattctgcac accataagac aggaaatcct gcggtgaaag
    16261 ttttttaaga tgttctggtg tttgtctttg attgttgatc attttatacc ctcatcaagc
    16321 ttttagtata gcccgtctac gccctaaaaa cattaaaaat cgtacacaaa gactagggtc
    16381 aaaaatttaa tacgcgcaga atggatgaaa gcaatgagtt ttgaccctaa gatctactcg
    16441 ccccgcttac ccttcttctc ctcagaatca gacgcttcga catcaatcgc cttgggcaac
    16501 acgccgtttt tcttgacacc ctgaatctcg atcttgcgag cgcttgattc cggaataacc
    16561 cgctccagat ctatatgaag caacccgtta tccagcgatg ccccgcgcac ctcaatccca
    16621 tcggccagca caaagctgcg ctggaattgt cgccccgcta tcccgcggtg caaatacaca
    16681 cgctcttcat cctcctcagg ctgccgcccg cggatcacca actgattact ctcaacttca
    16741 acatcgagag tctccatagt aaacccagcc acagccagcg taatccgcaa gccgctttcg
    16801 ccaatttgtt caatattata aggaggataa ccttctgcgc cagatttacg catacggtca
    16861 aatgtctttt caatttcgtc aaaccccaaa aaaagcgggc tgtcgaacaa cgtcaaacgt
    16921 cctgtactca ttgtcttgtc tcctcatatg agcgagttaa accatcatat atgcagccct
    16981 tcataggcac cgcgcaccaa ttatatagga ttttataaat ttgaatcaag catcgctgcg
    17041 atagcagctt tacgggccct ctttcacaaa caacctgtta caataactac actccacgct
    17101 atcccgatct tcaaaactgt accaaacctt cggatgacca aaagcacccc cgccaccatc
    17161 acacaaaacg ccatcgcacg cttcaggcac acttataatc tccgggcttt gcacatgcat
    17221 actatcagtc atattttcgt aaccctttta caatttatta gttcattaca ggttataaca
    17281 accctaagaa cactgtatta aaccacatct cggaaccaaa acaatcatca aatgaatgcg
    17341 aagaccgcca tagagtttac agacaacaaa accgcacaga tgcggccgga ccaatatatc
    17401 gaaatcacaa ttgatatagc gcgcacgctc gaaagctgga aaaactcact ctacagcttt
    17461 gaatggttgg aaaatgacgg ctcaattaaa cccttaaacg ccttaagcga aaacgaacaa
    17521 gccaaacgcc gggcagttga agaagcgctc gaaaccggac agcccctcga aaaaccgcta
    17581 ctgggcatcg gcatactcga caatatcgaa atcggctcag gacgggcagt tctcctcacc
    17641 ctggccgcta aagggcaaac aaccatgccc gtccatattc caaaatcttg cgaaagcgat
    17701 ttcaaagcgt ttcgcgctta agccgaattc agctataata aaaaaatgaa acacgcacgt
    17761 accaaacacc accttgatca tcaaaatgaa agtggcaatg tcctgtttta tatcctgatt
    17821 gctgtcgcct taattgccgc actatccttc gccgtggcca actccacccg cggcaatatc
    17881 aaacaactca acgctgaacg cgcacggctc tacgctaccg aaataatcga attcgccaac
    17941 gtcatagcct cagccgtcag ccagatccgc ctacgcggca cagatttaag cgccctgtgc
    18001 tttgaccacg caagctgggg ggcaagcgac tataaccacg ctggatgcgg cgacgacctc
    18061 aataaaatct tccaccccag cggagccgga ctaacatgga cacaggctcc ttccgaagct
    18121 atggattcga cagcaacgcc tgataatctc tggcacatat atggagataa cgaaattgaa
    18181 aatgtcggca ccacctgcgg tgcagccgct tgtgcagatc tgatcctgat cgtcgacgaa
    18241 ctccagcttg tcgtctgcca gcagctcaac gacctgcttg gcgtgacaga tgcctcgaca
    18301 accccgccaa ccgactccga ttttagcgaa acccgctata ttggcagctt cggctataat
    18361 gaaaccattg gcgatgaagc cggcggcgaa ccgcttgatg gcaagctcac cggatgcttc
    18421 cagaaaacaa acgctccagc cgaatatgtt ttctacaaag tcctggtcgc ccggtaaaag
    18481 caccctccgt cattgcgagc cgcaggcgaa gcaatccatc tcttaaggat tagattgccg
    18541 cgtcgagcta tcgctctcct cgcaataaca aaatgaacta agagattttc cgccacttcg
    18601 tcccttcaga cgtatcgaga atttcaatcc ccatagccgt caactgatca cggatttcat
    18661 ccgcccgcgc aaaattcttc tctacttttg ctacagccct ttcttgcagc aatacttcaa
    18721 tagagtcatt gtcgccgctt tctacatcac cagaaaaccc taaccattct tggggatttt
    18781 gctgcaatat ccccaataaa ttccccgcag caagaatctc acctttaagt tccggagaat
    18841 tctctttttt caaaagcgca ttcatctccg ccatcgcctg cggcgtattc aggtcatcac
    18901 ataaagcatc caaaacccct tgcggcacat ccgtttcaac cgcatccatc tccgccatct
    18961 catccagacg ggtataaaat cggtcaagct gtttccggct ttgctgcacc ttatcttccg
    19021 accagtccaa aggctgacga taatgcgccg acaacaacgt caaacgcaac gcctcacccg
    19081 gatatgtctc tatcaaatcg tgcacaagca aaaaattgcc cagcgatttg gacattttct
    19141 caccatcaac ggtga
//
LOCUS       DAOSWR010000021        18981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1835_length_18981_cov_5.874934, whole genome shotgun sequence.
ACCESSION   DAOSWR010000021 DAOSWR010000000
VERSION     DAOSWR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_18981_cov_5.874934"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="pyrB"
                     /locus_tag="PK513_02825"
     CDS             <1..598
                     /gene="pyrB"
                     /locus_tag="PK513_02825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468181.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOO81419.1"
                     /translation="RHAEFGAPKFVANRAGCPVINGGDSWREHPTQALLDALTMRQHF
                     GKIEGLKVTIVGDIAHSRVASSNMQLLTKMGAQVSIVAPKVLMPESLPFKEIKAYDSL
                     EDGLPGADVVMTLRLQKERMDKALISDAAYFNEFGLTFERLEKLCPKAMIMEPGPLVR
                     GVQMEDVVAEDPQRSLILRQVANGIPTRMAVLDMLVGE"
     gene            683..1339
                     /gene="plsY"
                     /locus_tag="PK513_02830"
     CDS             683..1339
                     /gene="plsY"
                     /locus_tag="PK513_02830"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOO81420.1"
                     /translation="MMSELEIFLVPYLIGSIPFGFLLAKLAGYGDIRKTGSGNIGATN
                     VLRTGNKFLAVFTLILDSAKGYIAALHFYLMVSLLFVIDNKPVPNTSDFSVEDSLLFG
                     LFAILGHCFPVWLKFKGGKGVATTFGVLFAAVPWTGFIAAVTWGIVAGFSRYSSLSAL
                     SAVAISPLVTFIYYGPMPAAITALIAALVIWRHKDNIKRLMAGTEPKIGQKKEEKEQE
                     "
     gene            1452..2597
                     /gene="dprA"
                     /locus_tag="PK513_02835"
     CDS             1452..2597
                     /gene="dprA"
                     /locus_tag="PK513_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO81421.1"
                     /translation="MQEQKLKNHPLSESDKLDWLRLSRTENVGPITFYRLIERFGSAE
                     KALDALPELSRRGGRTKPLKAPDLSTIEKEYESLREMGGDIVTAACPAYPLALGATDD
                     AAPVLSCFGNIQMANKNCIAIVGARNASLNGRKFAQKLAGDLGRLDQTIVSGLARGID
                     TAAHKGSLSTGTIAVVAGGIDVIYPQENLALFEEIKERGLILAESPLGTQPIARHFPK
                     RNRIVSGLSRACVVIEATMRSGSLITARMAGEQGRDVMAVPGHPLDPRAQGSNHLIRE
                     GATLVRNADDVLEIIRSFSGNSLREPFHPLPSFTQPAANDEDEPEDITEKARDRVLEN
                     LSFTPVTVDELLRSCHMTIPVLQTVLLELDLAGRIKREAGGRVSLLD"
     gene            2769..5336
                     /gene="topA"
                     /locus_tag="PK513_02840"
     CDS             2769..5336
                     /gene="topA"
                     /locus_tag="PK513_02840"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOO81422.1"
                     /translation="MLTTNLVIVESPAKAKTIGKYLGPDYEVLASFGHVRDLVNKDGS
                     VLPEDNFAMKWELGERSAKHVNDIKRAVKNADAIWLAPDPDREGEAIAWHVQEILSEE
                     NLLEGKALYRVTFNEITKSAVQDAFNHARTIDSPLVEAYLARRALDYLVGFNLSPVLW
                     RKLPGSRSAGRVQSVALRLICEREAEIEIFKAQEYWSIEARLHTQDGAPFTAKLTHLA
                     GNKLDKLDIGDEGAAKAAVERIENKNLRVQKIEKKQVRRNPPAPFITSSLQMEASRKL
                     GMSASQTMRCAQSLYEAGFITYMRTDGTTLSNEAVAQARDVIKSEYGAPYLPDAPRLY
                     KSKAKNAQEAHEAIRPTDLSKIPGKSGSPMGDEKERRLYELIWKRTMASQMENAVMDQ
                     MKVDISDGTDDAILRANGSVVTFDGFLTLYQESTETEDKDSDDDKGDDKDRRLPPMNE
                     GETSKLVDVMPNQHFTQPPPRYSEASLVKAMEEMGIGRPSTYASIMQVLRDRNYVEMQ
                     SRRFVPQDRGRVVTTFLTNFFTKYVDYDFTANLEEELDAIAGGHVDWKEALRLFWIDF
                     KAAVDETKELTITNVIDHLDGELGPHFFPEGDKSRKCQACGDGRLGLKLGKFGAFIGC
                     SNYPECKFTKPLVVPDGEEDGELAALANEPKELGTEPGTGRTVSLRRGPYGPYVQIDP
                     APEEAVDYTEYDAAIKVWEEAKKVTKEEGKKAPKKPTKPKAPKPKRQGLPKGLAVGDV
                     TLEAALSLLALPRDVGEHPETGKMIKAGIGRFGPFVQHDGKFASIPKDEDVMTIGMNR
                     AVDLIAQKLERDAAKDAAKAAKAAGDEDKKKAPAEKKKKAAKKKPAKKKTAAKKKSA"
     gene            5431..7710
                     /gene="rnr"
                     /locus_tag="PK513_02845"
     CDS             5431..7710
                     /gene="rnr"
                     /locus_tag="PK513_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOO81423.1"
                     /translation="MELNQSDILEFIENSPSPLTKREIARAFNVKGGENRITLKQILK
                     TLEAEGAVAKHPGGAYSVPDGLPSVCVLHVYEIDVDGDVFARPQSWNAELQGECPRIE
                     LRPGGKGHPAAKEGDRVLARLKRIGDNLYEAHTIKTLDSYEGRVLGQVRYHKHGPALV
                     PTDKKAKYDFDIKPGDLNGAQEGDLVIGEILPARGARRKKVKIVEVVGKQSDPKAISI
                     ISLHEAGLREDWPEAVLKDAEGLKVPDLKGREDLRDIPLVTIDGSDARDFDDAVFAEP
                     LEDGGFHLIVAIADVAYYVRAGSALDTEAQRRGNSTYFPDRVVPMLPEALSNDLCSLR
                     PHEPRACMAVHMWIDAHGQLKKYKFVRGLMRSAARLVYEQVQAAQDGVVDDMTGPLMN
                     TAIKPLYEAYAILDKARQKRGALDLDLPEKQILINDKNEMTGVKIRDRLEAHKMIEEF
                     MILANVAAASALEDKKDPKRFPCVYRIHDQPSADKLDSVREFLESFDLSLPKGQVTRP
                     AQINGILKRAEKLPYSHLISTVLLRSQAQAVYHPCNIGHFGLALHKYAHFTSPIRRYA
                     DLLVHRSLISAYGLGPGGISEEEIARIDELADHISTTERNSMMAERSAIDRFTAAYLS
                     EQIGAEFSGKISGVTRFGLFVTLDESGADGLVPMKSLKNDFYIHDEAQHALIGRRSGQ
                     VYRLGAAVSARLKEADGLTGSTVLELCGESMNGADIPGVTFKSKTGGLGRKPPSKGRG
                     PRGKKSKAFKKGKKDKRNA"
     gene            7768..7968
                     /locus_tag="PK513_02850"
     CDS             7768..7968
                     /locus_tag="PK513_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL33 family ribosomal protein"
                     /protein_id="HOO81424.1"
                     /translation="MAKAGYVKVRLESEAGTGYRYYAKRSTRAEYKIRKKKYDPWALN
                     EETGKKGMHVFFVEKKLPPHKK"
     gene            8133..9935
                     /locus_tag="PK513_02855"
     CDS             8133..9935
                     /locus_tag="PK513_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO81425.1"
                     /translation="MVLKKANKYRLDYTPDTNIPARLPGYVWMVVRRNPVMWAVFILM
                     DIIHGVRYPIAYLLVGLIIDMITELPAGSALPDAVWLYTAALFAVLFIGELTHAIPHY
                     FAFDWWKRARAELRSDLFAYTLQHSYTFFQDHFAGSLARKVSEGIEKALNLQEQIRWQ
                     ILLPVVSMGTSGLVLLKVSPLYGALVVAFLFILLLPVFLKLGKLREKSRIFADSCSDV
                     SGQIVDSLTNMASVKSYAHEARELEEHTRVSETQMKAWHKMLRAFLLLDNYRRMSLVV
                     FGSGMMVACVLGWQKGLITLGDIATIMGLSFNFTANAWNLSFGIIHVMESLGYLNDSL
                     KTLIKPHSVSDAPDAPALCVEQGAIEFRHVHFEYENKAVFTDLNLQIKAGQRVGLVGH
                     SGAGKSTLINLIQRFYDIQSGEILIDGQNIARVTQSSLRRNIAVIPQDTSLFHRSIMD
                     NIRYGRLDASDEEVLEAAKKAHAHGFIEELPEGFETHVGERGVKLSGGQRQRIAIARA
                     ILKDAPILILDEATSALDSESEQEIQAALQNLMADKTVIAIAHRLSTIQNLDRLLVMK
                     EGRIIEDGAHGALLAGGGVYQKLWAMQSGGFLPE"
     gene            complement(9945..11372)
                     /locus_tag="PK513_02860"
     CDS             complement(9945..11372)
                     /locus_tag="PK513_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HOO81426.1"
                     /translation="MSARVLVVDDILPNVKLLEAKLTSEYYEVLTAMNGEEALRKVAE
                     ESPDLVLLDVMMPGMDGFEVCTKIKQNSASAHIPVVMVTALTDVHDKVRGLEAGADDF
                     LSKPINDTALMARVRSLVRLKMSTDEWRIRENTANQLGVMDETTNAMSECPEKANILI
                     IEDKDFDLQKMSETLEKDSNTSRGVQSGAQAIEMISAHDFDLVIISLNLKNEDGLRLC
                     SHLKSNERTRAVPLLMVGGDEDMERIAHGLEIGAHDYILRPVERSELLARVRTQIRRK
                     RFQERLRATYEISLSMALTDSLTGLYNRRYLEVHLDKLLEKNKDSKKSLAVLMLDIDH
                     FKPVNDTYGHKAGDETLKIFAKRIQDNLRSFDLVARLGGEEFVAILPDISERRTYIVA
                     ERLRRSIADEPFDVGLESGPINITTSIGGTIVDFEKHTVEEVLERADKALYEAKEGGR
                     NCVVFEGKGKLIPEEHESEERKTIT"
     gene            complement(11387..11749)
                     /locus_tag="PK513_02865"
     CDS             complement(11387..11749)
                     /locus_tag="PK513_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81427.1"
                     /translation="MSKQVLIVEDNELNMKLFQDLLEAHDIETLKTSDGHDVLALARK
                     HKPDLILMDIQLPEISGLEVTKQLKSDDELKDIPVIAVTAFAMKGDEQKIREGGCEDY
                     ISKPISVGNFIEVIQSYL"
     gene            complement(11914..12756)
                     /locus_tag="PK513_02870"
     CDS             complement(11914..12756)
                     /locus_tag="PK513_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1849 family protein"
                     /protein_id="HOO81428.1"
                     /translation="MRILSLIFTFLIVSTSAHATEIQDKAQALGFQPHKALYDIKLAG
                     VRSGSQILNISGQMFYEWQPSCDAWTTNHRFNLLYEYADSAPMRITSNFSTYEPYDGA
                     SLNFTSQRKRDGNIFEEIRGSAEVKEDGMGNAVFTMPENLEFKLPTGTVFPIAHSMGL
                     AQKIREGAKFYTATIFDGSDEDGPVSVTAFIGKSAAPSQLGDEFPAIDKTLLGSPAHD
                     VRLAFFPLQDQEEISDYEMNLVFHENSLISDMLIEYDDFTVSQTLVAVEPVTDTCRHT
                     KFND"
     gene            13055..13813
                     /locus_tag="PK513_02875"
     CDS             13055..13813
                     /locus_tag="PK513_02875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO81429.1"
                     /translation="MASDMIQNEYVQRDKVPAGKANRLELTREQSFLDGQHDIFDILA
                     SHQNSKELLNQNLSETAHFLELTKHVLRSAEQKISEQDRSLQALQSLNERDQLSGLLN
                     RKGFSVLLAREVARTNRGHSVGGLLVMFNLENLQKIRSMHGMHTADLAVRLIASALES
                     EIRDMDLAARTEDDEFILLFTDTTMSQALSRLQNMALRLNRLSLIWDEAEIRLSLSLG
                     LKSYEKGARADAIFEGASDDLHRNRKYSAEVKRA"
     gene            13979..14638
                     /locus_tag="PK513_02880"
     CDS             13979..14638
                     /locus_tag="PK513_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81430.1"
                     /translation="MEIDLDNPDAFATSPVHVGLANKISVRDFHFTLQQILLMGDVGG
                     LSRASFSLPLNILTCEELEEKFRKIREARDRALKALAQMSFELGQDIIRVQNDIAESL
                     RVIDKKMNALESGDPGAVHLRKDRKALQEAQQNIETIDDRRIQATEPEQIENARVSLK
                     NLVESIRRSLERHEQDIPFNFKADEARRQPAEEPAFEIQDEESKPAPNQPSSGDETVP
                     P"
     gene            14697..16022
                     /locus_tag="PK513_02885"
     CDS             14697..16022
                     /locus_tag="PK513_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO81431.1"
                     /translation="MAEGKPEHQIHEIVWGDTLSELADRFGTTVDELVELNEIKNPDY
                     IRAGDTLKLPEVEPEPEQTAAPEASASSEEQFVEPPVVETVEISAAAKPPVEQSASAA
                     ALTNGFNGESGAKAAMGTSSEWQTFPSPASAGDAFSSAAEGEKNWAIRYGAEPFDAHV
                     FTKINRLVGSETLDKLNDVGNDIFHGGFAAFGNHLYMEFGTLNDDGEFEVQRRIHSFT
                     IADDGEGNKIIATENDGTLFTLASGGIIPFHEDPIYVMDHHKTKAGQLQSADDDFLFG
                     EGATKIVAQNLTKREAMGIYNVALKGGTDYNDRNIPVDGANVNGNTMHAGLRSLVEAA
                     AQTLGPEKMLDFNFDPSGWDAGLSYYNDLVEEGQKPSENMQIPQHTLSEYSTLQELEQ
                     EIVANEQKLAAQWQPSRDLAVNVNLTGKPDEKPELIPQHIKDFDPGPMA"
     gene            complement(16019..17857)
                     /gene="aspS"
                     /locus_tag="PK513_02890"
     CDS             complement(16019..17857)
                     /gene="aspS"
                     /locus_tag="PK513_02890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOO81432.1"
                     /translation="MHQYRTHTCAALRKTSVGETVRLSGWIHRIYDLGGVLFIILRDT
                     YGITQCVVEEGSPLMAELEKWRVESVITIEGAVAARTQETINSKMPTGEIEIRIEKAE
                     LQSAADVLPFQVHEDDGAGEDIRLRYRYIDLRREKMQKNIRLRNAVTASMRKRMWDQG
                     FQEFQTPILTASSPEGARDYLVPSRLHPGKFYALPQAPQQFKQLLMMSGFDKYFQIAP
                     CFRDEDGRADRLAEFYQLDVEMSFVTQDDVHGAIQPVIEGVFEEFADFTGEKRKVQWL
                     PSLPYKEAMLKYGTDKPDLRNPLEIVDVTEVFARDDVEFKAFKGTIAKGGVVRAIRAP
                     KVADQPRSFFDKLNSWAQGQGAPGLGYVLFEAGEGPIEGKGPIAKFIPAAAQDELRKI
                     AGLEDGDAIFFVCNLEGPAAKFAGAARDAICDALPEGHAAAREQGVYKFCWIVDFPMY
                     ELDEHTAKIEFSHNPFSMPQGGLKALNTQDPQDILAYQYDCVCNGFELASGAIRNHSP
                     EIMEKAFGLAGYDRAFLEAKFGGMLGAMRYGAPPHGGLAFGLDRIVMLLADEPNLREV
                     YAFVMNGQYEDQMMAAPSPVDEAQLKDLHLKLALPKKAVEADDKAA"
     gene            17959..>18981
                     /gene="rnd"
                     /locus_tag="PK513_02895"
     CDS             17959..>18981
                     /gene="rnd"
                     /locus_tag="PK513_02895"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOO81433.1"
                     /translation="MLITEQRPLEEFCASLKTCDFITVDTEFLREKTYYPKLCLIQIG
                     HPNGEAAAIDPLDHDLDLAPVYDLLRDEKILKVFHAGRQDLEIFFNLTGTVVAPLFDT
                     QIAAMVCGYGDSVGYENLVRSITGHSLDKSVQYTNWSNRPLSEKQIAYALGDVTYLVE
                     VYEKLAVELEKRGRTAWVFQEEDILADPATYANDPYEAWQRVKVKSPKPKTLAVLREL
                     AAWRERRAQEKDLPKSWVMRDETLADMAAQMPKDVKHLKKIRNMPGDLAESRHGKMLL
                     DLINVAINSNPKNWPQPKKKKPVPPSVSATVDILRMLLKIQAAQHDVATKLLSSTEDL
                     EAIAMED"
BASE COUNT         4728 a         4466 c         5219 g         4568 t
ORIGIN      
        1 gcgccatgct gagttcggcg cgccgaaatt tgtcgccaat cgggcgggat gcccggtgat
       61 caatggcggg gatagctggc gcgagcatcc gacgcaggct ttattggatg cgctgacgat
      121 gcgccagcat tttggcaaaa ttgaaggctt aaaggttacc atcgttgggg atattgccca
      181 tagccgggtc gcctcatcga atatgcagct tttgaccaag atgggggcgc aggtcagcat
      241 tgtcgcgccg aaagttttga tgcccgaaag cctgccgttt aaagagatca aagcttatga
      301 ttctctggag gacgggctgc cgggggcgga tgtggtgatg acgctgcgcc tgcaaaaaga
      361 gcgcatggac aaagcgttga tttccgatgc ggcgtatttt aatgaattcg gcctgacgtt
      421 cgagcggctg gaaaagctct gcccgaaggc gatgattatg gagccgggac cgctggtgcg
      481 cggggtgcag atggaagacg tggtggccga agacccgcag cgcagcctga ttttgcgtca
      541 ggtcgccaac ggcattccga cgcgcatggc ggtgctggat atgctggtgg gggaataaaa
      601 atgaaccgcg aagacgcaaa gacgcaaagt ttttttaatg tcatcccgag cgtagccggg
      661 tgatgacggg taagaggcta gcatgatgtc ggaattggaa atatttttgg taccttattt
      721 aattggttca atccctttcg gctttctttt ggcaaagctg gcagggtatg gggatattcg
      781 aaaaacaggt tccggaaata tcggggcaac caatgtttta agaacaggaa acaaatttct
      841 agccgttttt acccttatac ttgattctgc aaaaggttat atagctgcat tacattttta
      901 tttgatggtt tcattactat ttgtcataga taataaacct gtccctaata cttctgactt
      961 tagtgtggaa gatagtcttt tatttggcct tttcgccatc ctcgggcatt gtttcccggt
     1021 ttggttgaaa ttcaaaggtg gcaagggggt ggcgacgacg ttcggcgttc ttttcgccgc
     1081 ggtgccgtgg accgggttta ttgccgcggt gacttggggg attgtcgccg ggttttcgcg
     1141 ctattcatcg ctttcggccc tcagcgccgt ggcgatttca ccgctggtta catttatcta
     1201 ttacgggccg atgcctgccg cgataactgc gctcattgct gcgctggtga tctggcggca
     1261 taaagacaat atcaaacgcc tgatggccgg aaccgagccg aagatcggac aaaagaaaga
     1321 agaaaaagag caggaataaa gattgatgtc attgcgagcg cagcgaagca atccatctct
     1381 aaagcgtgta cgggagatgg attgccgcgt cggcctttca ggcctcctcg caatgacgag
     1441 aaagaaaaac catgcaagag caaaaactca aaaaccaccc tttatccgaa tctgacaagc
     1501 tcgactggct gcggctgtcg cgaaccgaga atgtcgggcc gattacgttt taccgattga
     1561 tcgaacgttt tggatcggcg gaaaaagctt tagacgcatt gcctgaactt tccaggcgcg
     1621 gtggacggac caagccgctc aaagcgccgg acctgtccac aatagaaaaa gaatatgaat
     1681 ccttgcgcga gatgggcggg gatattgtca cagccgcctg ccctgcatat ccgcttgctt
     1741 taggcgctac tgacgacgct gcgccggtgc tgtcctgttt cggcaatatt cagatggcga
     1801 ataaaaattg cattgcgatt gtcggggcgc gcaacgcttc gctgaacggg cggaaatttg
     1861 cgcaaaaact tgctggtgat ctgggcaggc tcgatcaaac aattgtcagc gggctggcac
     1921 gcgggattga tacggcggct cataaagggt ctttgagcac cgggacaatt gccgtggtcg
     1981 caggcgggat tgatgtcatt tatccgcagg aaaatttggc gctgtttgaa gagattaaag
     2041 aacgcggcct tattctggca gaaagcccgc tgggcacaca gcctatcgcg cggcatttcc
     2101 ccaagcgcaa tcggattgtt tcgggcctct cgcgcgcttg cgttgtgatt gaagcaacga
     2161 tgcgctcagg ctctctcatc actgcgcgca tggccgggga acaggggcgc gatgtgatgg
     2221 cggtgccggg gcatcctttg gacccgcgcg cgcaagggtc taatcatctg atccgtgagg
     2281 gggcgacgct ggtgcgcaat gccgacgacg ttctggagat cattcgtagc ttttcgggca
     2341 acagtttacg cgagcctttt cacccgctcc caagttttac acagcctgcg gccaatgacg
     2401 aagatgagcc ggaagatata acggaaaagg caagagatcg cgtgttggag aatttatcct
     2461 ttacccctgt tacggttgac gaattgctcc gatcctgtca tatgacgatt ccagtattgc
     2521 aaaccgtgct gctcgaactg gatctggccg ggcgcataaa acgcgaagcc ggcgggcggg
     2581 tgagtttgtt ggattagaga aatttaaacc acgaatggac acaaatgaac acgaattttt
     2641 aactgccaag tcaccaagat acgaggaaga ataaatataa aaaattaata cccccgcgct
     2701 ttgataattt tagtttttta ttcgtgtgaa ttcgtgttta ttcgcggttt atgaattaag
     2761 gatagtaaat tttgaccacc aatcttgtca ttgtagagtc cccggcgaag gccaaaacga
     2821 tcgggaaata tcttgggccc gattatgagg tgctggcctc gttcgggcat gtgcgcgatc
     2881 tggtgaataa ggacggttcg gtcctgccgg aggataattt cgcaatgaag tgggagctgg
     2941 gtgagcgctc ggccaagcat gtcaatgata tcaaacgcgc ggtgaaaaat gccgacgcca
     3001 tctggcttgc gcctgacccg gaccgggaag gggaagccat tgcctggcat gtgcaggaaa
     3061 ttttgagtga ggagaatctg ctggaaggta aggctcttta ccgcgtgacc tttaacgaga
     3121 ttacaaaatc agcggtgcag gatgccttta atcatgcgcg cactattgac agccccctgg
     3181 tggaagccta tctggcgcgc cgggcgctgg attacctagt tggatttaac ctttcacccg
     3241 tgctgtggcg caagctgccg ggatcgcgct cggcgggccg tgtgcagtcg gtggcgctac
     3301 gcctcatttg cgagcgcgaa gcagagattg agattttcaa agcgcaagag tactggtcga
     3361 ttgaagcgcg gctgcatact caagatggcg cgccttttac tgcgaagctc acgcatctgg
     3421 cgggcaataa actcgacaag ctcgatattg gcgatgaggg cgcggcgaaa gcggcggtcg
     3481 agcggattga aaataagaac ctgcgcgttc aaaaaattga gaaaaagcag gtgcggcgca
     3541 atccgcccgc gccgtttatt acttcatcat tgcagatgga agcctcgcgc aaattgggga
     3601 tgagcgcttc gcaaacaatg cgctgcgcgc agagcctgta tgaagccggg tttattactt
     3661 atatgcgtac ggacggcacg acactgtcga atgaggcggt tgcgcaagcc cgcgacgtga
     3721 ttaaaagcga atatggcgca ccgtatttgc cggatgcgcc gcggctgtat aaatccaaag
     3781 ctaaaaatgc gcaagaagcg cacgaggcga ttcgcccgac ggatctttcg aaaattcccg
     3841 gtaaatccgg cagccctatg ggagacgaaa aagaacgccg tttgtatgag ttgatctgga
     3901 aacgcacaat ggcttcgcag atggaaaatg cggtgatgga ccagatgaag gtcgatattt
     3961 cggacggcac ggatgatgcg attttgcgag ccaatggctc ggtcgtcacc tttgacggtt
     4021 tcctgacact gtatcaggaa agcacggaaa cagaagataa agattctgat gacgacaaag
     4081 gcgatgacaa agatagacgc ctgccgccta tgaatgaggg agagacttcg aagctggtcg
     4141 atgtgatgcc taatcaacac ttcacgcaac cgccgccgcg ctattccgaa gcctcgctgg
     4201 ttaaagcgat ggaagaaatg gggattggcc gtccgtcaac ctatgcctcg atcatgcagg
     4261 tgctgcgcga tcgtaactat gttgagatgc aatcgcggcg ctttgttcca caagaccggg
     4321 gccgcgtggt tacgacattc ctgaccaatt tctttactaa atatgttgat tatgatttta
     4381 ccgccaatct ggaagaagag ctggatgcga ttgccggcgg gcatgtggac tggaaagagg
     4441 ccttgcgtct gttctggatc gatttcaagg ccgccgttga tgagactaaa gagctgacga
     4501 ttacgaatgt gattgaccat ctggacggag aattggggcc gcacttcttt cctgaaggtg
     4561 ataaaagccg caaatgtcag gcctgtggtg atggacgttt aggcttaaag ctcgggaagt
     4621 tcggcgcgtt tatcgggtgt tcaaattatc cggaatgtaa gtttaccaag cctttggtcg
     4681 tgccggatgg cgaagaagac ggtgagctgg ccgcgctggc gaatgagccg aaagagttag
     4741 ggacagagcc gggcacgggc cgcactgtat ccttacgccg cgggccatat ggcccgtatg
     4801 tgcagatcga cccggcccct gaagaagcgg tggattacac ggaatatgat gctgcgataa
     4861 aggtttggga agaggctaaa aaggtcacga aggaagaggg taagaaagcg ccgaaaaaac
     4921 cgaccaagcc gaaagcgccg aaaccgaagc gtcaggggct gccgaaaggg ctggcggttg
     4981 gcgatgtgac gctagaagcg gcattgtctc tattagcatt acctcgtgat gtcggcgagc
     5041 acccggaaac cggtaaaatg atcaaggctg gaattggacg ttttggcccg tttgtgcagc
     5101 atgacggaaa atttgcctca atcccgaagg atgaggatgt gatgaccatc ggcatgaacc
     5161 gcgcagttga tctgatcgcc caaaagctgg aacgtgatgc agccaaggac gcggcaaaag
     5221 cggcgaaagc cgccggagac gaagacaaaa agaaagcgcc ggctgagaag aaaaagaaag
     5281 cggctaagaa aaaacctgcg aagaaaaaaa cagctgcgaa aaagaaaagc gcttaatttt
     5341 ctagccacag atggacacag atatacacgg attattcttt gatgttcaaa catctgtggg
     5401 catccgtgtt tatctgtggt tttttaaaat atggaattaa atcaatcaga cattcttgag
     5461 tttattgaaa actccccttc cccgttaacg aagcgggaaa ttgcgcgcgc atttaatgtc
     5521 aaaggcgggg aaaaccgcat tactctcaaa caaatcctca aaactctgga ggccgagggc
     5581 gcggttgcca aacatccagg cggagcttat agcgtgcctg acggcctgcc gagtgtttgc
     5641 gtcttgcacg tgtatgagat cgacgttgac ggtgatgtgt ttgcgcggcc tcagagctgg
     5701 aacgcggagc tgcaaggcga atgtccgcgt attgagctgc gccccggagg caaggggcat
     5761 ccggcggcga aagagggcga ccgggtgctg gcgcggctta aacgcattgg cgacaatctg
     5821 tatgaagcgc atacaattaa gacgctggac agctatgagg ggcgcgtatt aggtcaggtg
     5881 cgttatcaca agcacggtcc ggcgctggtg ccgacggata aaaaggcaaa gtacgatttt
     5941 gatatcaagc ccggcgattt gaacggcgcg caggaaggcg atctggtgat tggtgaaatc
     6001 ctgcccgccc ggggggcgcg ccggaagaag gtcaagatcg ttgaggtggt tggcaagcag
     6061 agcgatccga aagcaatcag catcatttcc ctgcatgagg cgggcttgcg cgaagactgg
     6121 ccggaagcgg ttttaaagga tgcggaaggt cttaaggttc ctgatcttaa gggccgcgag
     6181 gatttgcggg atattccact ggtcaccatt gacggaagcg atgcgcggga ttttgacgat
     6241 gcggtttttg cggagcctct tgaggatggc gggtttcatc tgatcgtagc gattgcggat
     6301 gtggcctatt acgtgcgtgc gggaagcgca ctggacacag aagcccagcg gcggggcaat
     6361 tcaacctatt tccccgaccg ggtggtgccg atgctgcctg aggctttgtc gaatgatctg
     6421 tgctctttac gtccgcatga gccgcgggcc tgtatggccg tacatatgtg gatcgatgcg
     6481 catggtcagc tcaagaagta taaattcgtg cggggcctga tgcgctcagc ggcgcggcta
     6541 gtgtacgaac aggtgcaggc agcgcaagac ggggtggttg atgatatgac cggtccgctt
     6601 atgaacaccg cgatcaaacc actgtatgag gcttatgcca ttcttgataa agcgcggcaa
     6661 aagcgcgggg cgctggatct tgatttgccg gaaaagcaaa ttctgattaa tgacaagaac
     6721 gagatgacgg gcgtaaagat ccgtgaccgg ctcgaggcgc ataagatgat cgaggaattt
     6781 atgattctgg ccaatgtggc ggcggccagc gctctggagg ataagaaaga cccgaagcgt
     6841 ttcccgtgcg tttaccgcat tcatgaccag cccagtgcgg acaagcttga tagcgtacgg
     6901 gagtttctgg aaagctttga tctgtctttg cccaagggac aagtgacgcg gcccgcgcag
     6961 attaacggca ttttgaagcg agcggaaaaa ctgccctact cgcatttgat ctcgacggtg
     7021 cttttgcgtt cacaagctca ggctgtctat catccgtgta atatcggaca tttcgggctg
     7081 gcgcttcaca aatatgcgca ttttacctca cccatccggc gttatgccga ccttctggtg
     7141 catcgtagcc tgatcagtgc ctatgggctt gggccgggcg ggattagcga agaggaaatt
     7201 gcgcgcattg atgagctggc cgatcatatc tccacgacag agcgcaattc tatgatggcc
     7261 gaacgcagtg ctatagaccg gttcacggcg gcgtatttgt cggagcagat cggggctgaa
     7321 ttttcgggga agatcagcgg ggtgacacgg tttgggctgt tcgtcacact ggatgaaagc
     7381 ggcgcggacg ggctggtgcc gatgaaatcg cttaaaaatg atttttatat ccatgatgag
     7441 gctcagcatg cgcttatcgg gcggcgctca gggcaggtat atcggctggg agccgctgtc
     7501 agtgcgcgct taaaagaagc ggatgggcta acaggttcaa cggttctgga gctgtgcggt
     7561 gaaagcatga atggcgcaga tattcccggc gtcacgttca agtcaaaaac cggcggactg
     7621 gggcgcaaac cgccgtccaa agggcgtggc cccagaggaa aaaagtcgaa agctttcaaa
     7681 aaaggaaaaa aggacaagcg taatgcttga catttcgggc aattttacgc ataaacgcct
     7741 ctcgaataag aatttaggag atgagtgatg gcaaaagccg gctacgtcaa agtgagattg
     7801 gaaagcgaag caggaacggg ataccgttat tatgcaaaac gctcaacgcg cgcagagtac
     7861 aaaattcgta agaaaaaata tgacccgtgg gctctgaatg aagagacagg gaaaaagggt
     7921 atgcacgtat tttttgttga gaaaaagctg ccgccgcata agaaataaag agctgttttt
     7981 taagattttt tgaaaagcgc ctttatggcg cttttttgtt tttgggttta atgcgtcatt
     8041 gcgaacctgc gtagcaggtg aagcaatcca tacaaatttg gattgccgcg tcggaacttc
     8101 gttcctcctc gcaatgacaa ggaaagatga aaatggtttt aaaaaaagcc aataaataca
     8161 gactggatta cacgccggat accaatattc cggcgcggtt gccgggctat gtctggatgg
     8221 tcgtgcggcg taacccggtg atgtgggcag tatttatcct gatggacatc atccatgggg
     8281 tccgctatcc gattgcctat ttgctggttg gcttgatcat cgacatgatt acagaactgc
     8341 ctgctggcag cgccctgcct gatgctgtct ggctgtatac agcggccttg tttgctgtgc
     8401 tgtttatcgg cgagctgaca catgccatcc cgcattattt tgcgttcgac tggtggaaac
     8461 gcgcccgcgc ggagctgcgc agtgatttat ttgcgtatac tttacagcat tcatatactt
     8521 tctttcagga ccattttgca ggatcgctgg cgcggaaagt ctcggaaggg atagagaaag
     8581 ctttgaattt gcaagagcaa atccgctggc aaattctact gccagtggtc agcatgggga
     8641 cctctggcct tgttttgcta aaagtttctc cgctttatgg cgcgctggtc gttgcgtttt
     8701 tatttatttt gctgctaccg gtattcctaa aactcggaaa attgcgagag aagtcccgta
     8761 tctttgccga tagctgctcg gatgtttccg gacagatcgt tgacagcctg accaatatgg
     8821 cctctgtcaa atcctatgcc cacgaggcgc gggaactgga agaacacacc cgcgtgagtg
     8881 agacgcagat gaaggcgtgg cataagatgc tgcgtgcgtt cttgctgctg gataattacc
     8941 ggcggatgag tctggttgtc tttggcagcg gaatgatggt tgcttgcgtt cttggctggc
     9001 agaaggggct gatcacgctg ggcgatattg caaccattat gggcctgagt tttaacttta
     9061 ccgccaatgc gtggaactta agctttggga ttattcatgt gatggaatcg ctgggttatc
     9121 tcaacgattc tttgaagacg ttgatcaagc cgcacagcgt cagtgatgcg ccagatgcgc
     9181 ctgcgttgtg tgtcgaacaa ggcgcaatcg aatttcgcca tgttcatttc gaatatgaga
     9241 ataaggccgt atttaccgat ctcaatcttc agattaaagc tgggcagcgc gttgggctgg
     9301 tcgggcattc cggcgcaggg aaaagtacgc tgatcaattt gatccagcgt ttttacgata
     9361 ttcaaagcgg tgaaatcctg attgatgggc agaatattgc ccgcgtcacg caaagttcgt
     9421 tgcgccggaa tatcgctgtt attccgcagg atacatcgct gtttcaccgc agcatcatgg
     9481 ataatatccg ctatggacgg ctggatgcca gtgatgaaga ggttttagag gcagcgaaga
     9541 aagcccatgc gcatggtttt attgaagaac tgccggaggg gtttgaaacc catgtcgggg
     9601 agcgcggcgt caagctatcg ggcggccagc gccaacgcat tgcgattgca cgggcgattt
     9661 tgaaagatgc gccgatctta attctggatg aggccacgtc cgctctggat agtgaaagcg
     9721 aacaggaaat tcaggcggcg ctacaaaatc tgatggcgga taaaactgtg atcgcgattg
     9781 cccaccggct ttcgacaatc cagaatcttg atcggctttt ggtcatgaaa gaaggccgga
     9841 ttatcgaaga cggcgcgcat ggagcattgc tggccggcgg cggagtttat cagaagctct
     9901 gggcgatgca atcgggcggg tttttgccgg agtagcaatg gtggctatgt gatcgtctta
     9961 cgctcttcgc tttcgtgctc ttcggggatc agcttgccct tgccctcaaa cacgacgcag
    10021 ttacgcccgc cttccttggc ttcgtaaagc gctttatcgg cacgttccag tacttcttca
    10081 accgtgtgtt tttcaaaatc gacaatcgtg ccgccgattg aggtggtaat attgatcggg
    10141 ccactttcga ggccaacgtc aaaaggttcg tcagcaatgg agcggcgcag ccgttcggcc
    10201 actatatagg tacggcgctc tgaaatatcc ggcaaaatgg caacaaactc ttcaccgccc
    10261 agccgtgcga cgagatcaaa actccgcaag ttatcctgaa tgcgtttggc aaaaatcttg
    10321 agcgtctcat cgccggcctt atggccgtat gtgtcattca ccggtttgaa atggtcaata
    10381 tcgagcatca aaacggccag tgatttttta ctgtccttgt ttttttcaag cagcttatcg
    10441 agatgaactt ccagatagcg acgattatac agaccggtca agctgtcggt cagagccata
    10501 gaaaggctga tttcgtaagt cgcacgcagg cgttcctgga aacgtttgcg ccgaatttgt
    10561 gtgcgtacac gcgccaacag ttcggaacgt tcaaccggac gcaagatata gtcatgcgcg
    10621 cctatttcca gcccgtgagc aatgcgctcc atatcctcgt cgccaccaac catcaaaagc
    10681 ggcactgcgc gagtgcgttc gttcgacttt aagtgggagc atagccgcaa gccatcttca
    10741 tttttaagat tcaggctgat gatcaccaaa tcaaaatcat gggcagagat catctctatg
    10801 gcttgtgcgc cgctttgtac accgcgactt gtgttgctgt ctttctcaag tgtttcggac
    10861 attttttgca gatcgaaatc tttgtcctca atgatcagta tatttgcttt ttccggacat
    10921 tcactcatcg cgttggtggt ttcatccatc acgccgagct gattggccgt attttcacga
    10981 atgcgccatt catcggtaga catttttaac cgaacaagcg agcgtacgcg cgccataaga
    11041 gcggtatcgt taatgggttt actcaagaaa tcatcagcgc cggcttccaa gccgcggact
    11101 ttgtcgtgca catcggtaag cgccgtcacc atcacgacgg gaatatgcgc actggctgag
    11161 ttttgcttta ttttggtgca aacctcaaag ccgtccatac ccggcatcat aacgtcaagc
    11221 agaactaaat cggggctttc ctccgccact ttacgcagag cttcctcgcc gttcatggct
    11281 gtgagaactt cgtaatattc gctcgtcagt tttgcttcca aaagcttgac gttcggcaaa
    11341 atatcatcaa ccaccaacac acgtgcggac attgcttccc ctgtttttat aaatagcttt
    11401 gtatgacttc aatgaaattt ccaaccgata ttggctttga aatataatct tcacagccac
    11461 cttcacggat tttttgttca tcgcctttca tggcaaacgc ggtgacggcg attaccggaa
    11521 tatctttaag ctcgtcatcg ctttttaact gctttgtcac ttccaaaccg gaaatttccg
    11581 gtaactgtat gtccataagg atcagatccg gcttgtgctt acgtgcaagg gccaaaacat
    11641 catgtccatc actggttttg agtgtttcta tgtcatgcgc ctcgagaaga tcctgaaaga
    11701 gcttcatatt cagctcgtta tcttcaacga ttaaaacttg tttactcatt gtttttttgc
    11761 ccttataccc cgatcggcaa ggcgatgcgg gtgccctata ttcaagaata gaagttcttt
    11821 gtttccaaag tattaattta aagattaata ttattatctg cttatggatt ctatcggttt
    11881 ttagtgtgtt tgagaagccg ctttgtttct tgcctagtcg ttgaatttag tgtgcctgca
    11941 ggtgtcagtt acaggctcca ccgctacgag ggtctgtgag acggtaaaat catcatattc
    12001 gataagcata tcgctaatca gactgttttc atggaatacc aaattcattt cataatcgga
    12061 tatttcctcc tgatcctgaa gcgggaaaaa ggccaaacgc acgtcatgtg caggagaacc
    12121 cagcagggtt ttatcgatcg ccggaaattc gtctcctaat tgactgggcg ctgcggactt
    12181 cccgataaag gctgttacac ttaccggacc gtcctcgtca ctgccgtcaa atatcgtggc
    12241 cgtatagaat tttgcacctt cacgaatttt ctgcgccaaa cccatgctgt gggctattgg
    12301 gaacactgtg cctgtcggca gcttgaattc cagattctcc ggcatggtaa agaccgcatt
    12361 gcccatgcca tcttccttca cttcagcgct tccgcgaatt tcttcaaaaa tattgccatc
    12421 gcgcttgcgc tgcgaggtaa aattcaagct tgcgccatca tagggttcat aggttgaaaa
    12481 attgctggta atgcgcatcg gtgcgctatc agcatattca taaaggagat taaaacggtg
    12541 gttggtggtc caggcatcgc aggatggctg ccattcataa aacatttgac cagagatgtt
    12601 gagaatttgc gaaccgctac gcacccctgc cagtttaata tcgtacagtg ccttgtgcgg
    12661 ctgaaaacct aaggcttgcg ccttatcctg gatttcagtc gcgtgggcag acgtggaaac
    12721 aatcagaaat gtgaatatca gggacaatat gcgcatgggg aagctttctt gacagtgcag
    12781 cgcaatcata gcaaagggat gcgtatgggc aaagagctaa tcatgcgtga tacaggcact
    12841 ttttgcgcgg caaactctac cctatgacag gcggaaaaat aatccatttt ttacagaatc
    12901 ttattatccg gtgcttttcc tcaaaacaga ccttaacgca agcaattgat ttaaaacaaa
    12961 tttagcagaa acaaaactgg cacaagattc gcaaccataa gatttatatg acatttgcgg
    13021 ttttattgac ccaacacggc agaaggagag cttgatggct tcggatatga tccagaatga
    13081 atatgtgcag cgcgataaag tgcccgccgg taaagctaac cggcttgagc ttacgcgcga
    13141 gcaatcgttt ttggatggtc aacacgacat attcgatatt ttggcctctc atcagaatag
    13201 caaagagttg ttaaaccaaa atttgagtga aactgctcat tttctggaac tcacaaaaca
    13261 tgtgctgcgc agtgccgagc aaaagatttc cgagcaagac cgaagcttac aggcgttgca
    13321 aagcttgaat gaacgtgatc aactcagcgg gcttttgaac cgcaaaggct tttcggttct
    13381 gctggcccgc gaagtcgccc gtacaaatcg cgggcatagc gtcggcgggc tgttggtcat
    13441 gtttaatctg gagaatttgc aaaaaatacg cagtatgcac ggtatgcata cggcggatct
    13501 ggcggtgaga ctcatcgcca gtgcgctaga aagtgaaatt cgtgacatgg atctggcggc
    13561 gcgaacggaa gatgatgagt tcatcctgct ttttacagat acgacaatga gtcaggccct
    13621 gagccgttta caaaatatgg ccctgcgact taatcggctt tcactgattt gggatgaggc
    13681 tgaaattcgt ttaagcctta gcctcggcct caaaagttat gagaaaggcg cacgtgccga
    13741 tgccattttt gaaggcgcca gcgacgacct tcaccgtaat cgaaaatatt cagcggaggt
    13801 taaacgtgcg taactatttt tgaattttca atgcctgcag ctcctccccc aaccgtgaac
    13861 cctaaaatgc aggcaacacc cccaaagccc ggctctgtac cccttaaaat gagccgggct
    13921 tttccttttt tgacaaaaag cccgcaaatc aggtatttta caaaaatcaa aataaagaat
    13981 ggagatcgat ttggataatc ctgacgcctt tgctacgagt cccgtgcatg tcggacttgc
    14041 gaacaaaatt agcgttcgcg attttcactt tacgctgcaa caaatcctcc tgatgggcga
    14101 tgtgggcggc ttgtcacgtg cgagcttttc cctgccgctt aatattctta cctgtgaaga
    14161 gcttgaagag aaattcagaa aaatcagaga ggccagggac cgggcgctca aggcgctggc
    14221 gcagatgtct tttgaactgg gacaggatat tatccgtgtg cagaacgaca tcgccgaaag
    14281 tctgcgcgtg attgacaaga aaatgaacgc tctggaaagc ggtgatcccg gagccgtaca
    14341 cctgcgcaag gaccgaaaag ccttacaaga agcacaacag aatattgaaa ctattgatga
    14401 caggcgcatc caggccacag aacctgaaca gattgaaaac gcaagagtca gcctgaaaaa
    14461 tctcgtagaa agtattcgcc gctctctgga gcgccatgaa caagatatcc cgtttaactt
    14521 caaggcggat gaagcgcgcc gtcaaccggc agaggaaccg gccttcgaaa ttcaggacga
    14581 agagtcgaaa cccgctccca atcagccctc ctccggagac gagaccgtcc ccccttaaat
    14641 taacatattt ttaacacacc ctctttagaa taaagaaaaa caaaaaaggg tgtgttatgg
    14701 ccgaaggaaa acctgaacat caaatacatg aaattgtctg gggcgatacg ttatcagagt
    14761 tggccgacag gtttggcaca actgttgacg aattggttga actcaacgag ataaaaaacc
    14821 ctgattatat tcgtgcaggt gatacgctga aattgccgga ggttgagccg gagccggagc
    14881 aaacagcagc gcctgaagcg tccgcttctt ctgaggaaca gtttgtcgaa cctcctgttg
    14941 ttgagactgt cgaaatctcg gctgctgcga agccgccagt tgagcagtcc gcatctgctg
    15001 cggcattgac aaacggtttt aacggtgaat ccggtgccaa agcggcaatg ggtacctctt
    15061 cagaatggca aactttccct tctcccgctt ccgcagggga tgcgttttcg agcgccgccg
    15121 agggtgagaa aaactgggct attcgttacg gcgcagagcc ttttgatgct catgttttca
    15181 ccaagatcaa cagattggtt ggatccgaga ctcttgataa gcttaatgac gttggaaacg
    15241 acatttttca cggtggcttc gccgcttttg gtaatcatct ctacatggaa tttgggacat
    15301 tgaacgacga tggtgaattt gaagttcaaa gaagaattca cagctttact attgccgacg
    15361 atggcgaggg caacaagatc atcgccacag agaatgatgg aacgcttttt actcttgctt
    15421 caggtgggat tattcctttc catgaagatc cgatctatgt catggaccat cacaaaacaa
    15481 aagccggtca gctacaaagc gctgacgatg attttctgtt tggggaaggc gcaaccaaaa
    15541 tcgttgcgca aaacctgaca aaacgcgaag ccatggggat ttataatgtc gcgctgaaag
    15601 gcggaaccga ctacaacgac agaaacattc ctgttgatgg tgcaaatgtt aatggcaata
    15661 ccatgcatgc agggctgagg tccttggttg aagcggcagc acaaactctt ggccctgaga
    15721 aaatgctcga ttttaatttt gacccctcgg gatgggatgc ggggctatct tattataatg
    15781 atctcgtcga agaagggcag aagccgtctg aaaatatgca gattcctcaa catacgctga
    15841 gcgaatattc aaccctgcaa gaactcgaac aggaaattgt tgcaaacgaa caaaaactgg
    15901 ctgcgcagtg gcaaccatcc agagacctag cggtcaatgt caacttgaca ggaaagcctg
    15961 acgaaaaacc ggaactcatc ccgcagcata ttaaagattt tgatcccggg ccgatggctt
    16021 aggccgcttt gtcgtctgct tcgacggcct ttttcggcaa ggccagtttc agatgtaagt
    16081 ctttgagctg ggcctcatca accggagatg gcgcggccat catctggtct tcatactggc
    16141 cgttcatcac aaacgcataa acttcgcgca ggttcggttc atcggccagc agcatgacaa
    16201 tgcgatcgag gccaaaggcc agcccgccgt gtggcggcgc gccgtagcgc atggcgccga
    16261 gcatcccgcc gaatttggct tcgaggaagg cgcggtcgta cccggcgagg ccgaaggctt
    16321 tttccatgat ctcggggctg tggttgcgga tcgcgccgga ggccagttcg aagccgttgc
    16381 acacacagtc atactgatag gccagaatgt cctgcgggtc ctgcgtattg agcgctttta
    16441 agcccccttg cggcatggag aacgggttgt gcgagaattc aattttggcg gtgtgttcgt
    16501 caagttcata catcgggaaa tcgacgatcc agcagaattt ataaacgcct tgttctcgtg
    16561 cggcggcgtg accttcgggc agagcatcgc aaatcgcatc gcgcgcggcg ccggcgaatt
    16621 tggccgccgg gccttcgagg ttgcagacaa agaatatggc atcgccatct tccagcccgg
    16681 caattttgcg cagctcatcc tgtgcggcag cggggatgaa tttggcgatc gggcccttac
    16741 cctctatggg gccctctccg gcttcaaaca ggacataacc gaggcccggt gcgccctggc
    16801 cctgcgccca gctattgagc ttgtcaaaga agctgcgcgg ctggtcggcg actttggggg
    16861 cgcggatggc gcggacaacg ccgccttttg caatcgtgcc tttgaaggct ttaaactcaa
    16921 cgtcatcgcg ggcgaagact tcggtgacat cgacaatttc gagcgggttg cgcaggtcgg
    16981 gcttatcggt gccgtatttg agcatggctt ctttatacgg caaagaggga agccactgga
    17041 ctttacgttt ctcaccagtg aaatcagcga attcctcgaa cacgccttcg atcacaggct
    17101 ggatggcacc atgaacgtca tcttgcgtaa caaagctcat ttccacgtca agctggtaaa
    17161 attcggccag acggtcggcg cggccatctt catcacggaa acatggggcg atctggaaat
    17221 atttatcaaa gccgctcatc atgagaagct gtttgaactg ctgcggggct tgcggcaagg
    17281 cgtagaattt ccccggatgc aagcgtgagg ggacgagata gtcgcgcgcg ccttcggggc
    17341 tggaggcggt caggatgggg gtttggaatt cctgaaagcc ttgatcccac atccgtttgc
    17401 gcatagaggc ggttacggcg ttgcgcaggc gaatattctt ttgcattttc tcgcggcgca
    17461 aatcgatata gcggtagcgc aggcggatat cctcaccggc cccatcgtct tcgtgaacct
    17521 gaaacggcag cacatctgcc gcgctttgca actccgcctt ttcaatgcga atttcaattt
    17581 caccggtggg cattttcgaa ttaatggtct cttgcgtacg ggcggcgact gccccttcga
    17641 tggtgataac gctttcaacg cgccattttt caagctcagc catcaggggc gagccttcct
    17701 ctactacgca ttgcgtaatg ccgtaagtgt cgcgcaggat gataaataaa accccgccta
    17761 agtcatagat acggtgaatc cagccggaaa gcctaaccgt ttcgccgaca ctggttttgc
    17821 gcaaagccgc gcaggtgtgt gttctgtatt ggtgcataat tgtcttaatc ctttaattcc
    17881 cagccgtttt ttaaccttac aatgccgaag acgcaaaggt tttattgcat tttttattga
    17941 aggtagtaaa cacagtgcat gcttattacc gaacaaagac ccctcgaaga gttttgtgcg
    18001 tccctgaaga cctgtgactt tatcactgtc gataccgaat ttctgcgcga aaaaacctat
    18061 tatccgaagc tttgcctgat ccagatcggg catccgaatg gggaggctgc ggcgattgat
    18121 ccgctggacc atgatcttga tctcgccccc gtatacgatc tcttgagaga tgagaaaatt
    18181 ctcaaggttt ttcacgcggg acgacaggac ctggagattt tttttaatct gacgggcaca
    18241 gtcgtggcgc ctttgtttga tacacaaatc gcggcgatgg tatgcggata cggtgattct
    18301 gtcggctatg aaaatctggt gcgtagcatt actggacata gtcttgataa gagtgtgcaa
    18361 tacaccaatt ggtcgaaccg cccgctaagc gaaaagcaaa tcgcttacgc gcttggcgac
    18421 gtgacctatc tggttgaagt gtatgaaaaa ctggcggtgg agctagagaa gcgcgggcgc
    18481 accgcgtggg tgtttcagga ggaggatatt ctggccgatc cggcaactta tgcgaatgat
    18541 ccgtatgaag cctggcagcg tgtgaaggtg aaatctccaa aaccgaagac attggcggtg
    18601 ctgcgcgagc ttgccgcttg gcgagaacgc cgcgctcaag aaaaggattt accgaaaagc
    18661 tgggtaatgc gcgatgaaac gctggcggat atggcggcac aaatgccaaa agatgttaag
    18721 cacctgaaaa aaatcaggaa tatgcccggc gatctggccg aaagccgaca tggcaaaatg
    18781 ttgctggatc ttattaatgt tgctattaat agcaacccta aaaactggcc gcaaccgaag
    18841 aagaaaaaac ctgtaccacc aagtgtctcg gcaacagttg atattttgcg catgctgctc
    18901 aaaattcagg cggcgcagca tgatgtcgct acaaagttgc tatcttctac tgaagaccta
    18961 gaggctatcg caatggagga t
//
LOCUS       DAOSWR010000022        18898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1846_length_18898_cov_5.562649, whole genome shotgun sequence.
ACCESSION   DAOSWR010000022 DAOSWR010000000
VERSION     DAOSWR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18898
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_18898_cov_5.562649"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PK513_02900"
     CDS             <1..319
                     /locus_tag="PK513_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81434.1"
                     /translation="MNIAGCMPFIMELWTTDMSMSKALSIIVDPMAIIVIYSAAGVGY
                     LIDWAVTMLVANFLYQRGGSRKNTIEKRQAELIERWGEEVNGKIPIDHEGFPVEGSAG
                     EGT"
     gene            449..1003
                     /locus_tag="PK513_02905"
     CDS             449..1003
                     /locus_tag="PK513_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOO81435.1"
                     /translation="MINIGVVLAKFLKKFIKNEKGTTAIEFSLLAIPYLMLTLGIIEI
                     AIMYASASLLEGATNSASRMIRTGELQQSGADPETAFRDGLCDYTTVLINCNDVVVES
                     LTLSSFSDVATSPAQFDADGNMVSQGFDAGGSDARILIRVAYRYEMMTPFVGRLISGS
                     DNSRLFMSTIVLQVEPYDFAGAGA"
     gene            1000..1524
                     /locus_tag="PK513_02910"
     CDS             1000..1524
                     /locus_tag="PK513_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOO81436.1"
                     /translation="MIVNFVKCWFKSEDGVAAVEAALVFPILLTLLLGLVDLGNGILA
                     NQKAIRASQVAADLIARSSVVDTSMIDDALDASELAFQPFDTTTYGVDVVSVSFDDDA
                     NPVIEWRETRNMTPLADVEVRVEDLAQAGEGVLVVAVEYQFEPMFAGFLIEEIPMQEI
                     AFARGRQGAVIERN"
     gene            1572..3038
                     /locus_tag="PK513_02915"
     CDS             1572..3038
                     /locus_tag="PK513_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOO81437.1"
                     /translation="MLKRMFLNFVRYYRDTRAAIAILFALMAPLLIGTVGMAIDLSQA
                     YLVQQRLAQAIDAAALAAAAISSDEATIEQKVQDFFDVNYPPEKLGVTFDPVVVIEGN
                     LVTVTGNASYLTSFLRVLGIDNIDVTAETVVQREVKGLEVVLVLDNTGSMASNNNIGK
                     LRTAACEFVEILYGTYDEDASGDCLDRYGSYVTAQNEYVKIGLVPYATSVNVGPYGLG
                     YDDDGNVYADPFLNNPLGLSFSNDADTNICILEEDDGSDVLDHSGPWYMYRWCRDKND
                     DSAQCNYYNNYICVGDQYNSSGSTCLDWDYSAGARTVYRAPEYSCPAASVLPLTDDMT
                     ALKDRIGEMEANGWTFGNIGMAWGWRMLSPEVPFTEGVDWDNTAWRKVVVMMTDGDNV
                     RSSPYGGFGINGTHSVSSSSVLDDRLRDVCENMDAQDNVTVYTITFDTGGIDSTTEQL
                     YEDCAGNGGSHKHVTTSDELIQVFRSIAQELSNLHIKS"
     gene            3460..3639
                     /locus_tag="PK513_02920"
     CDS             3460..3639
                     /locus_tag="PK513_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOO81438.1"
                     /translation="MLKLLALKDAYLRNEDGATAIEYGLIAAGIAIAIVAVVFTLGDE
                     ISATFQDVEDELAAR"
     gene            3718..3918
                     /locus_tag="PK513_02925"
     CDS             3718..3918
                     /locus_tag="PK513_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOO81439.1"
                     /translation="MKKFLQIYVCFSAFIQAFWLERKAATAIEYALMAGGIALVIIAV
                     VFAIGDELAVLFQEVEDGFSGP"
     gene            3953..4501
                     /locus_tag="PK513_02930"
     CDS             3953..4501
                     /locus_tag="PK513_02930"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO81440.1"
                     /translation="MLLLIVFLMCLFVVIGTGGLSALSDLRGMTIPNLHSLIVLGAFV
                     FCYGALWFGGRDDVFASLASHIVAALIVFLITLAMFMGKLMGAGDSKLASALALWAGL
                     KGLVPFIFYMSVAGGVLALAALALKKWAPVKAPKPESWVAQVQGGASKVPYGVAIVLG
                     ALASFVKIGYFDLDVLSSFVVE"
     gene            4670..5554
                     /gene="cpaB"
                     /locus_tag="PK513_02935"
     CDS             4670..5554
                     /gene="cpaB"
                     /locus_tag="PK513_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOO81441.1"
                     /translation="MNKNILIVLGGAVLVAVLVAMLVQVTLGGKKEAAALQEAKVEVL
                     VASKDLVLGRELKEGDMRWQEWPKSSVFPGAVLRQDEQKPEEALEGRLGRNVAKGEPV
                     MKSAMLGQVKGNLVAATLEPGQRAIAIEVSASTMVGGFIGPGDFVDVILTYKESIRTE
                     DDNPQITQMIELNLDKMATETILQNVKVLAVDQLAERPEDDKIKVGKTVTLAVGAQDA
                     ERLSLASQLGELTLVLRAVGDDVLVEKKWPTISDARLTSMGDEVYAEYEKMKKDAGIN
                     RNIVRIYSGEQVTAIPAR"
     gene            5631..7106
                     /locus_tag="PK513_02940"
     CDS             5631..7106
                     /locus_tag="PK513_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HOO81442.1"
                     /translation="MSTFLSRIRAALALAAVFVVFMAPLAVTAAKGDLKTLQSEMKIK
                     APLTITLGKADLVSVEGDVSDVLVADPSIVDVMAVQSNSLYVVGVNVGDTNIIALDAM
                     GNIVKRIDIHVAYDLQAIQSLVNELYPEETVKVGAIHDQILLTGTVSNPDVASKITNI
                     VGHYVSDLQDEDDPIDELISNLLEVRGEQQVMLQVKIMEASRTALKELGMETYMNDPN
                     ELSTTTLFGGNPGSSVTSRGDQFTFGTGGGIALSQDAAGIAGGLIESGLRGVGLIGLE
                     LNALEERNLVNILAEPNLTAVSGEQAGFLAGGEFPVPAGRDQVGNIIIEFREFGVSLN
                     FRPVVLSGKRISLQMNTEVSSLDFENAVTLADLVVPGLDIRRADTTVEIPSGGSLMIA
                     GILRSDALEGMTGLPGISKTPVLGDLVKSDSFQRSETELVVIVTPYLVEPYAEEDRAA
                     PVPKQKNNPLAQAFAANIRRVYDVEDEAIFALDEQFGYLLD"
     gene            7119..7787
                     /locus_tag="PK513_02945"
     CDS             7119..7787
                     /locus_tag="PK513_02945"
                     /inference="COORDINATES: protein motif:HMM:NF021025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HOO81443.1"
                     /translation="MSVRTSKKHRWTVSLPYTILGMITLAMVLSGCLSEPTMLNQNRV
                     QVQEETFTDTLPASELSGSAVQGLARHYDQHGGGPLELTVTYDPKSRSANAMHANDEA
                     ARIVKELRAAGVHSVDANILPVNGSGGMNAMVSYTAYNALAPKDCSTIPGFEDLDVKV
                     DEDYRLGCTTETLFARQVARPKDLKGQGTVDPITDGRRMSNTVAVYRMGVPNEPLDGE
                     SASE"
     gene            7881..9134
                     /locus_tag="PK513_02950"
     CDS             7881..9134
                     /locus_tag="PK513_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO81444.1"
                     /translation="MSEVDSQATSILLPASSVAVYSKDQKTLQAARDLENDWRFARVS
                     VQDEEGDVENAIATYQDIASPTLIIIQTDVIDKSFTARLEELAGHCEAGTSAIIVGPD
                     NDVNLYRRLIDMGVSDYLVRPISSADLAKVVSKALIEKIGVTGSRLVAVMGAKGGVGS
                     SIISEALACGLTDILGQKAILLDASGGWSTLAVGLGFEPSTTLSEAVRAAENADEDSL
                     KRMLYTVSDKLSVLATGGDVMLEQGVSPDQLETLIDMLMVKYPFVIVDLSQSAAALQK
                     IILTRANQILVTATPTLPALRLARSLIQEVKEVRGGEDSDVSLIVNMQGQAPANEVSK
                     SDIEKAMEFSVAAYLPYDAKMFLGNESESRKLTDDKVARDLIKKTLLPVLQKSLTEIT
                     TEGAEEGGEEGGFLGNLLGKLKSKG"
     gene            9178..10851
                     /locus_tag="PK513_02955"
     CDS             9178..10851
                     /locus_tag="PK513_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOO81445.1"
                     /translation="MFGKKTSDQGGSASSPKPKVEKKPAPKEVKVEEKKSAPVSAPKS
                     DPEPVRDEKPQGGEADNPAAMLAGLGDALEKKSDDKAVVGEKAAFVPENPKSVSKKKV
                     EGEKGKPTAKDPFSEEMAPAEEEGENSTDSITTLRLQRSRNRIWLDLRDGIDLKALAN
                     MEAKAAREEVLSAVEEIARFRNLDLTAAELQSIAKECADDMLGFGPLEELLEQDGIAD
                     IMINGPDTTYIEINGKIEKAKIKFRDNQHLTTICQRIVGAIGRRVDEASPICDARLPD
                     GSRVNVIIPPLAVDGACMTIRKFTKDKLTLDKLQQFGSMTPSCAKLIMAIGRCRVNVL
                     VSGGTGSGKTTMLNCLTRYIEPGERIITCEDACELQLQQPHVVRLETRPPNLEGVGEV
                     TMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTVHANNPREALSRME
                     NMIAMGGLNLPQQAVREQIAGAVQVIIQVQRLRDGSRKTTHVTEITGMEGEVVTMQDL
                     FVLDIEGEDEHGKLVTKHRSTGLRPKFFDNARQFGVDGMVLEAMEGAFD"
     gene            10894..11868
                     /locus_tag="PK513_02960"
     CDS             10894..11868
                     /locus_tag="PK513_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO81446.1"
                     /translation="MSVVQIVLITLIAILVMGIIVAYMAAQESTRKKRVMGVIQGKSG
                     GARGPDERGSKDRRRADLAKKLKEAKGEENSKKKTTISTMILQAGISISVKQFWLMSV
                     ALMIGLTFLAKLMGQPPHVLIAVAVIGLLGLPRFVLKKMAARRQKKFLEEFPDALEAT
                     VRLLKAGMPVSEAIAMISREFTGPVGEEMGNIYDKQKIGVALAEAALEATRSMPLTEM
                     QMFATGLSIQAQTGSSLSEVLTNLSNVIRARFRLKRKIKALSSEAIASASIIAALPVL
                     VATGMYFVNYEYISVLFTDPFGNVLLAIAVFWMSVGVMVMKIMINFKV"
     gene            12004..12948
                     /locus_tag="PK513_02965"
     CDS             12004..12948
                     /locus_tag="PK513_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO81447.1"
                     /translation="MDNELIITLVSALLAAGSFAAFALPFLNKTEKKEHYRSVVDKRR
                     KDLFQATRDGSIHKAKTEKNVSAADSMAALYKMQQIAGAMGEKVRDKMLQAGIRSPKA
                     PIKFMIAQAVVPIVLAGLTVLVLSNTEKEISDMMKLLSVFTAAVIGYKLPDVLIKNTI
                     LKRQEEIGLAFPDSLDMMLICVQGGIGLEQTVDRVAEEVAEHSPVLAEELGLLSAEMA
                     MLNDRREALQDFARRVGSGAAKSFATSLIQAEQYGTSISQAMRVMSDELRDMRMAKAE
                     QKAAALPPKLTVPMILFFLPALFIIILGPAGISASSLN"
     gene            complement(13043..13759)
                     /locus_tag="PK513_02970"
     CDS             complement(13043..13759)
                     /locus_tag="PK513_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO81448.1"
                     /translation="MSIKKTTSLILTLALCSALTACQTAQSTDAQLRTSSMDGAIERA
                     ANYTRKPSKSLSYLERTYKKQPDSELAAVDYAVGLRENSKYELAATILEPFAKGKNAS
                     SLATSEYAAIQLAQGNYEAAEKYAQKAVIADETNFKAYHRLGIALDTQGMHQEAERAY
                     RKGIELWQGDPTTIMNNLALNLASQGYLNESIEILRKAQDVSPGRVEIERNLRIITAL
                     QQADGTPAPKPGKKPITTTD"
     gene            complement(13841..14458)
                     /locus_tag="PK513_02975"
     CDS             complement(13841..14458)
                     /locus_tag="PK513_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOO81449.1"
                     /translation="MIFSKYLKFALFSAIVLLQSLSVNVPSFAQENGSQPPLDILPAT
                     TAQERGIIYDDQTHTHPAIKLTPDKSELVHLDRPAASIIVGNPNHLSILADTSQTLVL
                     VARAPGATQFTVLDKNGAIVMQRHVIVASPKEKYMRIRRSCAGSDDDTCQSTSVFYCP
                     DMCHEIIMGSEEADSNAGALVEGGNTNTNEADALTSPPAEINDTE"
     gene            complement(14736..16493)
                     /locus_tag="PK513_02980"
     CDS             complement(14736..16493)
                     /locus_tag="PK513_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81450.1"
                     /translation="MSATAAAQLYTPAPSLRDHWQRAGTVAERAKTALQNRIHKITDN
                     PLTKTFAKAAMGAGAAYAVKTAAISLASTAGSGAVAAAVLGGAAVTTYKAAKDYRTQN
                     KDRPHGFWANLKGFFSFLGQNKAKYAKQLAISSGLGIIGAAGLVETFAHAANAGEIDD
                     LIMPENIANHYQPPEQGITTPPAEMPATEATEPQTAVTQTEPQKIAATPALTPLEKLS
                     QINPDNFSGRAAETLSYAKKGHAWAVKEMAIHLLSGNNGSPLNREFAVELLQIAEHSD
                     NPKIANQASRDLDTIKNLWPEAFAQEEITIAEAPEFEPTTSEVAEKPKAQALKEHIVT
                     AEAPASPLERLAALNPENFGQRGQYVLQFAEKGHGWALNELASSLLYEGNGSPQDRKL
                     AVELYRLAQETSNARIVEHATKDLAVVERLWGVDNIMGTSAPNEPKTLLAGGKPTPQE
                     LPMPKLPANTNIPVLCNAASTETGIDIKCVLDPSQIKSSDTFTINLPGNAEMNFYYDG
                     KAANLGITEPGDAIASPNRTSNIVNLNLHFSHEAMQNPEAMYSITAGNNAYGISFSPQ
                     SEPPIQYAKAADGPKLGAL"
     gene            16657..17853
                     /locus_tag="PK513_02985"
     CDS             16657..17853
                     /locus_tag="PK513_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81451.1"
                     /translation="MSNVTNSRVIAGTLVVAMSGAAQAGGYGGGDEIGEQNNYYGDDY
                     ELIMNGGSPSAAATAGLEMEGSLNPSAAANNHANVTGRVDSRNTLTGNNSLEVERIGS
                     DSRANVNIAPGAIQPVANGGAAEQEQTNGNNEIQVDDHSINKIDARQGAATNGMAARL
                     ETARFLAQACESRFSVEGRLGDVFGNYAGAGISYHPSLKFVIQNKKTGAIFSGQQLWD
                     AAPVDRGYMTYGMDEDTIALAMCFAEALYGTGDRVHRHIVTEEAQRHNWERSKNLPGV
                     KDEEKASVAEHMQAHMYVMGANSVYRDAMGCTMTSYVNEGGTGEINQKDRPTPTQVMM
                     NMFGLKKPTKDQSTGRVAQQRAKLIPECEFKTTIPEPPKNEGAGNGKVSDESGTNNIF
                     DVLSPN"
     gene            17980..18828
                     /locus_tag="PK513_02990"
     CDS             17980..18828
                     /locus_tag="PK513_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81452.1"
                     /translation="MAAFKHMADFSSFANDNFVRGYGARALRVLAPVSLAVLLSACAS
                     TKEVMEESTTPNCVDQQTYTKFISKTVKEDVEVSSKDGSSKVEQREKTVDKIVERFRI
                     NKGFNEQCEAHKILVLTAEAQQDNEVGIIASMALLFLASGQDEATHQAITEMLQRYDS
                     SLEKLTVRVDEFDKKRAIDFIDTQVGDLYHGNGQPNYPAVAAILGLYDQDSQPEGKRR
                     YPKLEQAFLREVIDGALVARGLDIAELKQMYEGARRKIGCTSEKIGRYMQTRCEVQED
                     LPVYGP"
BASE COUNT         4362 a         3362 c         5589 g         5585 t
ORIGIN      
        1 catgaatatc gccgggtgta tgccttttat tatggagctg tggacaacgg atatgtctat
       61 gtccaaggca ctatcgatta ttgtggaccc gatggcgatt attgttattt attctgctgc
      121 tggtgttggg tatttgattg attgggcggt gacgatgttg gtggctaatt ttctttatca
      181 acgtggtggt tcacgcaaaa atacgattga aaagcgacag gctgagctta ttgagcggtg
      241 gggcgaagag gtgaatggga aaattccaat agaccatgaa ggttttccgg ttgagggtag
      301 cgctggggaa ggtacttaaa atcctatcta tacttaaata aatgttcata tataggcgtc
      361 atactggagg tgttgggtta ttgggtattt atgaagcgtg cgctagccgc tttttttatg
      421 ccataaattt aaaaccaatt taatttaaat gataaatatt ggggttgtgt tggctaaatt
      481 cttgaaaaaa ttcattaaaa atgaaaaagg tacaacggca attgagttta gcctgctggc
      541 aattccgtat cttatgttga cgcttggtat cattgaaatt gcgattatgt atgcatcggc
      601 cagtttgttg gaaggcgcta cgaattctgc atcacggatg attaggaccg gtgaattaca
      661 gcaatcgggc gcagatccgg aaactgcgtt ccgggatggt ttgtgtgatt atacgacggt
      721 gttgataaac tgcaatgacg tggttgttga atctttgacc ttgagcagtt tttccgatgt
      781 ggcgacatcc cctgcgcaat ttgacgccga tggcaacatg gttagtcagg ggtttgatgc
      841 cggtggttct gatgccagga ttttgatccg ggtggcatat cgctatgaaa tgatgacgcc
      901 gtttgttgga aggctgattt caggttctga caattcacgc ttgtttatgt caacaatcgt
      961 tttgcaagtt gagccgtatg atttcgcagg ggcgggggca tgatcgtgaa ttttgtaaaa
     1021 tgctggttta aatctgaaga tggtgtggcc gccgttgaag cggcgttagt ttttccaatt
     1081 ttgttgacgt tattgcttgg attggttgat ttaggaaatg gaattttggc caaccaaaag
     1141 gctattcgcg cttcgcaggt tgcggcggat cttatcgcac gcagcagcgt tgttgatacg
     1201 tcgatgattg atgatgcact ggatgccagc gagctcgcgt ttcagccgtt tgatacgact
     1261 acgtatgggg tggatgttgt cagtgttagt tttgatgacg atgctaaccc agtgattgaa
     1321 tggcgggaaa cgcgtaatat gacgcctttg gcggatgtgg aggttcgggt tgaggatttg
     1381 gcgcaagccg gtgaaggggt tttggttgtt gcggttgaat atcaatttga acccatgttt
     1441 gccggttttc ttattgaaga aatcccgatg caggaaattg cctttgctcg cgggcgtcag
     1501 ggcgcggtta tcgaacggaa ttaaaaatgg gctgtatagt ccttaaaaaa gtaaaaacta
     1561 aggtggttgt tatgcttaag agaatgtttt taaattttgt gcgttattat cgcgatacgc
     1621 gcgcggcgat tgcgatcctt tttgctttga tggccccctt gttgatcggg acggttggta
     1681 tggcgattga tttgtcgcag gcttatcttg ttcagcagcg cttggcgcag gcgattgatg
     1741 cggcggctct ggcggcggcg gctatttcca gtgatgaggc gacgattgaa caaaaagttc
     1801 aagatttttt tgatgttaat tatccgccag aaaaattagg cgttacgttt gatcctgttg
     1861 ttgtcattga gggaaatctg gtgacggtga ccggtaatgc gagttattta acctcgtttc
     1921 tgcgggtgct tggtattgat aacattgatg ttacggccga gacggttgtg cagcgtgagg
     1981 tgaaggggct ggaggttgtt ctggttctgg ataacaccgg ctcgatggcg tcgaataata
     2041 atattggcaa gctccgtacg gcggcatgcg agtttgtaga aattttatat ggtacatatg
     2101 atgaggatgc gtctggtgat tgcttggatc gttatggctc ctatgttacg gcgcagaacg
     2161 aatatgtgaa gatcgggctg gttccttatg ctacatctgt gaatgtcggt ccttacgggt
     2221 tgggctatga cgatgatggt aatgtgtatg ccgatccttt cttgaataat cctctggggc
     2281 tgagtttcag taatgatgcg gatacgaata tctgtatttt ggaagaagat gacggtagcg
     2341 atgtgctgga tcatagtgga ccgtggtata tgtatcgctg gtgccgggat aagaatgatg
     2401 atagcgcgca gtgtaattat tataacaatt atatctgcgt cggtgatcag tataactcgt
     2461 ccggttcaac ttgtctggat tgggattatt ctgctggagc cagaacggtt taccgggcgc
     2521 ctgaatatag ttgtccagcg gcgtctgtgt tgcctttaac ggatgatatg acggcgctca
     2581 aagaccggat cggagaaatg gaagccaatg gctggacatt cgggaatatc ggtatggcct
     2641 ggggttggcg aatgctttct cccgaggtgc cgtttaccga aggggttgat tgggataata
     2701 ccgcctggcg caaggtggtc gttatgatga ccgatggtga taatgtgcgc tccagccctt
     2761 atggtggttt tggtataaac ggtacgcatt ctgtcagcag ttccagtgtt ttggatgatc
     2821 gcctgagaga tgtttgtgaa aatatggacg cacaggacaa tgtgactgtc tatacgatta
     2881 ccttcgatac gggggggatt gattcaacca cggagcagct ctatgaggat tgtgccggta
     2941 atggcggctc gcataagcat gtgacgacgt ctgatgagtt gattcaggtg tttcgcagta
     3001 tcgcgcagga actcagcaat ctgcatatta agagttaaac gcataaaaga ttatgcgtga
     3061 caggaagcct tgctgatgtg atctgcaagg ctttttgagt ggaatattag taaaggcctg
     3121 gtgttttatt gtgctttatt tttgttttta aaaactgctc agaaaaagtg aaataaaaca
     3181 gagtcttttt gagttgttta agtattttat aagtaaatat aaagtaaata tcagtattga
     3241 aaactattta aaattttatt taaaaaagat tcaattttat acttgaaggg tggtgatttt
     3301 ttattgtatt attgctgcaa atactgcgtt ttttaacctt ccattaatca actttcgata
     3361 ttctaaaaga gtaaacggtt gggagcttca agtccttaag tctcattggt tttgaagtgt
     3421 gggtaaccgg aattcgaaaa aatctaaaag gagaaaacca tgttgaaatt gcttgctttg
     3481 aaagatgctt atcttagaaa cgaagacggt gcaacggcta ttgaatacgg ccttattgct
     3541 gccggtattg cgattgcgat tgttgcagtt gtgtttacgc tcggtgatga aatctcagcg
     3601 actttccaag atgttgaaga tgaacttgct gcacgttaat atgtgctgat gttttaggaa
     3661 aagcccatcc ttggcgggtg ggctttttta ttgttctttg tatgtgggtt tctgcacatg
     3721 aaaaaatttc ttcaaatcta tgtttgtttt tctgctttca ttcaggcgtt ttggctggag
     3781 cgtaaagcgg cgacagcaat tgaatatgct ttaatggcag gtgggattgc tttggttatt
     3841 atcgcggttg tgtttgcaat tggtgatgag ctcgctgttc tttttcagga ggtcgaggac
     3901 ggtttctccg gtccttaact ttttcaatta ttcctttttc agggtttcga ttatgcttct
     3961 tcttatcgtc tttttgatgt gtttgtttgt ggtgattggt acgggcgggc tgtcggcgtt
     4021 gtcggatttg cgcggtatga ctattcccaa cctgcacagt ttgatcgtgc tgggtgcatt
     4081 tgtgttttgc tatggtgctt tgtggtttgg tgggcgcgat gacgtgtttg cgtctttagc
     4141 ttcacatatt gttgcggctc ttattgtgtt tttgatcacg ctggcgatgt ttatgggtaa
     4201 attgatgggt gctggagatt ccaagttggc aagcgcgctt gcgttatggg ccggtttaaa
     4261 gggattggtg ccgtttatct tttatatgag cgttgcaggc ggcgttctcg ctttagcggc
     4321 tctggcactt aagaaatggg cgcctgtgaa ggctcctaag cctgagagtt gggtggcgca
     4381 ggtgcaaggc ggggcgagta aggttcctta cggcgttgca attgtgcttg gcgcgctggc
     4441 atcttttgta aaaatagggt attttgatct ggatgtttta tcatcgtttg ttgttgagta
     4501 gttccttaag cgcctgtttt tctgcttttc tttgtaattt tttaggtttt acgctttttt
     4561 aagaaaatag gcgcatcata aaaatagact atttattttg cctgtttcgg gctttgctgc
     4621 gttcttttgc tatattttca ataatacaaa aaccggatga ggttttgcta tgaataagaa
     4681 tattctcatt gttcttggag gtgctgtgct tgtggctgtg ttggttgcca tgcttgtgca
     4741 ggtgactttg ggtggcaaga aggaggcggc agccttgcag gaggctaagg ttgaggttct
     4801 tgtggcgtca aaagatctgg tgcttggacg tgagttgaaa gagggggata tgcgttggca
     4861 ggaatggccg aaaagcagcg tgtttcctgg tgcggtttta cgtcaggatg agcagaagcc
     4921 tgaagaagct cttgaggggc gtttggggcg caatgttgcc aaaggtgagc ctgttatgaa
     4981 gagtgcgatg cttgggcagg tgaaaggtaa tttggttgcg gcgactttgg agcccggtca
     5041 gcgcgcgatt gcgattgagg tttccgcgtc aactatggtg ggtggtttta taggccctgg
     5101 tgattttgtc gatgtgattt tgacttataa ggaaagtatt cgcaccgagg atgataatcc
     5161 gcaaattacg cagatgattg agttgaacct cgataagatg gcgacggaga cgatcttgca
     5221 gaatgtgaag gttcttgcgg ttgatcaact ggccgagcgt cccgaggatg ataagattaa
     5281 ggttggtaaa actgtgacgc tggctgtagg ggctcaggat gctgagcgtt tatctctggc
     5341 ttcccagctt ggcgagttga ctctggtgct gcgcgctgtt ggtgatgatg tgcttgttga
     5401 aaagaaatgg ccgactatat ctgatgcgcg cctgacgagt atgggcgatg aagtttatgc
     5461 cgaatatgaa aaaatgaaaa aggatgctgg aattaatcgt aatattgtgc gaatctatag
     5521 cggtgagcaa gtcacggcaa ttcctgctcg ttaaatctct tagcattctt tgctgtcaat
     5581 tttgtttctc ttctctgcgt aactaactga aagtcttata gatgatgacg atgtccacat
     5641 ttttatccag aattagggcg gcgctggctt tggcggcggt gtttgttgta tttatggccc
     5701 ctttggcggt tacggcggcc aaaggtgatt tgaaaacgct ccagagcgaa atgaaaatca
     5761 aggcgccatt aacgattacg cttggtaaag cggatttggt ttcggtcgag ggagatgttt
     5821 cggatgtgtt ggttgcggat ccttcgattg ttgatgtcat ggctgtgcag agtaacagct
     5881 tgtatgtcgt tggtgtgaat gttggtgata cgaacattat tgcgctggat gcgatgggga
     5941 atatcgtgaa gcggattgat attcatgtcg cgtatgattt gcaggctatt cagtcgcttg
     6001 tgaatgagct gtatcccgaa gagactgtta aggtcggggc tattcatgac cagattttgt
     6061 tgacggggac ggtttctaat cctgatgtgg cttccaagat taccaatatt gtcgggcatt
     6121 atgtaagtga tcttcaagat gaggatgatc cgattgatga attgatttcc aatcttttgg
     6181 aagtgcgcgg cgagcagcag gtgatgttgc aggttaagat tatggaagct tcacgcacgg
     6241 cgcttaaaga gctggggatg gaaacgtata tgaacgatcc gaacgagctt tcaacgacga
     6301 ccttgtttgg cggtaatccc ggtagtagcg tgacgtcgcg cggggatcag tttacgtttg
     6361 gtacgggcgg cgggattgcg ttgtctcagg atgcagcggg gatcgcaggt ggtcttatcg
     6421 agagcggctt gcgtggtgtt ggtctgattg ggctggagct taatgctctt gaagagcgta
     6481 atttggtcaa tattctggct gagccgaatt tgacggcggt gtccggtgag caggccggct
     6541 ttttggccgg tggtgagttt cctgtgcctg ctggacgtga tcaggttggg aatatcatta
     6601 ttgagtttcg tgagttcggc gtatcgttga atttccgtcc tgtggtgttg tccggcaagc
     6661 ggatcagtct gcagatgaat acggaagtgt cttcacttga ttttgaaaat gcggtgacgc
     6721 tggctgatct ggttgtgccg ggtctggata ttcgccgggc ggatacgact gtggaaatcc
     6781 ccagtggtgg cagtttgatg attgccggga ttttacgttc tgatgcgctg gaagggatga
     6841 ccggtttgcc ggggattagt aagacgcctg ttcttggcga tcttgttaag tctgacagct
     6901 ttcagcgtag cgaaactgag ttggttgtga ttgtgacgcc atatctggtt gagccttatg
     6961 cggaagaaga ccgtgcggca cctgtgccga agcagaaaaa taatccgttg gcgcaagcgt
     7021 ttgctgccaa tattcgtagg gtctatgacg ttgaggatga ggcgattttt gctctggatg
     7081 agcaatttgg atatttgctg gattaagggg tgattgatat gtctgtgaga acaagcaaaa
     7141 aacatagatg gacggtgtct ttgccttata cgattttagg tatgattacg ctcgcaatgg
     7201 ttttgagcgg gtgtttgtct gagccgacga tgcttaatca aaaccgtgtg caagtgcagg
     7261 aagagacatt taccgatacg ttgccggctt cggaattgag cggatcggct gtacaaggct
     7321 tggccaggca ttacgatcag cacggtggcg ggcctttgga gttgacggtg acgtatgatc
     7381 ctaaatccag atcggctaat gcgatgcatg ccaatgatga agctgcgcgc attgtgaagg
     7441 agttgcgcgc tgccggtgtg catagcgtag atgcaaatat attgccggtg aatggttctg
     7501 gcggcatgaa tgcgatggta tcttatacgg cctataatgc tttggctccg aaggattgca
     7561 gtacaattcc aggctttgaa gatttggatg ttaaggttga tgaggactat aggcttggtt
     7621 gtacgactga aacattgttc gcgcgtcaag ttgcccggcc taaggatctt aaaggacagg
     7681 gcacggtcga tcctattacc gacggtcgcc gcatgagtaa tacggttgct gtgtatcgca
     7741 tgggtgtgcc taatgagccg ttggatggtg agagcgcgtc agaatagggc tgaagactgc
     7801 tcataaagtg ttaaacaaat ggaactataa tttaaatggc tttaaataag catgacggac
     7861 gaggataaaa ataataatca atgagtgagg tagacagtca ggcaacctct atactgcttc
     7921 ctgcatcgag tgttgctgtt tattcaaaag atcaaaagac attacaggcc gccagagatc
     7981 ttgaaaatga ttggcggttt gcgcgcgtta gtgtgcagga tgaggaaggt gatgtcgaga
     8041 atgctattgc gacgtatcag gatattgctt ctccgacact gattattatt cagaccgatg
     8101 tgattgataa aagctttaca gcgcgtcttg aggaattggc cggacattgt gaagcaggca
     8161 cttcggcgat tattgtcggg ccggacaatg atgtgaattt gtatcgtcgt ttgattgata
     8221 tgggggtaag tgattatctc gtgcgcccta taagttcagc ggatttggct aaagtcgttt
     8281 ccaaggcctt gatcgaaaag ataggtgtaa cgggaagccg tctggttgcc gtgatggggg
     8341 cgaagggtgg tgtgggatcg tctattattt ctgaagcgct ggcttgcggt ttgaccgata
     8401 ttttggggca aaaggcgatt ttgcttgatg catccggcgg atggtcaact ttggcagttg
     8461 gtttgggatt tgagccgagc acaacgctga gcgaggctgt gcgcgcggcg gaaaatgctg
     8521 atgaggatag cttgaagcgt atgctttata cggtgagtga caagcttagt gtgctggcaa
     8581 ccggcggtga tgtgatgttg gaacaggggg taagcccaga tcagctcgaa acgctaattg
     8641 atatgttgat ggtgaaatat ccgtttgtga ttgttgattt gtctcaatct gcagcggcgc
     8701 tacagaaaat tattttaacg cgggcaaacc agattttggt gacggctacg ccgactttgc
     8761 cggctttgcg gcttgcacgc agtcttattc aggaagtgaa agaggtgcgc ggcggagaag
     8821 atagcgatgt tagcttgatt gtcaatatgc aggggcaggc acccgctaat gaagtgtcaa
     8881 agtctgatat tgaaaaggcg atggagttca gtgtagcggc gtatttgccc tatgatgcga
     8941 agatgttttt gggaaatgaa agtgagagcc gcaaattgac cgatgataag gttgcgcgcg
     9001 atttgattaa aaagacgttg ttgccggttt tgcagaaatc tttaacggaa attacaactg
     9061 aaggcgctga agagggcggt gaagaaggcg gctttttggg taatttgctt ggcaagttaa
     9121 agtctaaagg gtgatggcat cgcgcgggct tttaagtttt aagtaggacg tatttctatg
     9181 tttggaaaga aaacttcaga tcagggcggt tctgcatctt cgccaaagcc gaaggtggag
     9241 aaaaagcctg cgccgaaaga ggttaaggtt gaagagaaga agtctgctcc tgtatctgca
     9301 ccaaaatcag atccagagcc ggtgagggat gaaaaaccgc aaggcgggga ggctgataat
     9361 cctgcggcga tgttggctgg attaggggac gcgcttgaga agaagtccga tgataaggca
     9421 gtggttggag agaaggctgc ttttgttcct gaaaatccta aatctgtttc caagaagaag
     9481 gtagaggggg aaaagggaaa acccacggca aaagatccat tttctgagga gatggcgccg
     9541 gcggaagaag agggggaaaa tagtacggat tctataacga cgcttcggtt gcagcgttca
     9601 cgcaatcgaa tctggcttga tttgcgtgac gggattgatc tgaaagcttt ggcaaatatg
     9661 gaggccaagg cggcgcgtga agaagttttg agcgcggttg aggaaattgc gcggttccgt
     9721 aatctcgatt tgactgctgc ggagttgcaa agtattgcca aggaatgcgc cgacgatatg
     9781 cttggttttg gtcctttgga agagcttttg gagcaagacg gtattgccga tattatgatt
     9841 aacggaccgg atacgaccta tattgagatt aacggtaaga ttgaaaaagc gaagattaaa
     9901 ttccgtgata accagcattt gacgacgatt tgtcagcgga ttgtgggagc aatcggccga
     9961 cgagttgatg aagccagtcc gatttgtgat gcgcgcttgc cggatggcag ccgtgtaaac
    10021 gtgattatcc cacctttggc tgtggacggc gcgtgtatga ccattcgtaa atttaccaag
    10081 gataagctga cgcttgataa gttgcagcaa tttggatcaa tgacgccgag ttgtgcgaag
    10141 ttgattatgg cgattggtcg ttgccgggtg aacgtgctgg tctctggcgg tacggggtcc
    10201 ggtaagacga cgatgctgaa ctgtttgacg cgttatattg agcctggtga acggattatc
    10261 acatgcgaag atgcatgtga attgcagctg cagcagccgc atgttgtgcg tcttgaaacg
    10321 cgcccgccaa acctggaagg ggtgggtgag gtgacgatgc gcgatttggt aaaaaactgt
    10381 ctgcggatgc gcccggagag gattattgtt ggtgaggtgc gcggcccgga agcatttgat
    10441 ttgctgcagg cgatgaatac aggtcacgat ggttcgatgg gtacggtgca cgctaacaac
    10501 ccgcgcgagg ctttgagccg gatggaaaat atgattgcaa tgggcggttt gaatttgccg
    10561 cagcaagcgg tgcgtgaaca gattgcgggt gcggtgcagg tgattattca ggtgcaacgt
    10621 ttgcgtgacg ggtcgcgaaa aacaacgcat gtgactgaga ttaccgggat ggagggcgag
    10681 gttgtcacca tgcaggatct ttttgtgctg gatattgagg gtgaagatga acatggcaag
    10741 cttgtgacta aacatcgttc tacaggtttg cggcctaaat tttttgataa tgcgcgtcag
    10801 tttggggttg acgggatggt gctggaagct atggaaggcg ctttcgacta aaaatttatc
    10861 tgcccataat agatttaata aggtcttgag tttatgtcag ttgtacaaat tgttttgatc
    10921 acattgatcg ctattttggt gatggggatc attgttgcct acatggcggc gcaggaatct
    10981 acgcgcaaaa aacgggttat gggcgtgatt caggggaaaa gtggcggtgc tcgcgggcct
    11041 gacgagcgtg gatcaaaaga tcgccgacgg gcggatttgg caaagaagct aaaagaggcc
    11101 aagggtgaag aaaattctaa aaagaaaacc acaatttcga cgatgatatt acaggccgga
    11161 attagtattt ccgtgaagca gttctggttg atgtctgtgg cgctgatgat agggttgacg
    11221 tttttggcaa agcttatggg gcagccgccg catgttttga ttgctgtagc tgtgattggt
    11281 ttgcttggtc ttccacgatt tgtacttaag aaaatggccg cgcggcggca gaagaaattt
    11341 ttggaagagt ttcccgatgc attggaagca actgtgcgtt tgttgaaggc cgggatgccc
    11401 gtttccgaag ccatagctat gatttcccgt gagttcaccg gccctgtggg agaagagatg
    11461 gggaatattt atgataagca gaagatcggt gtggctttgg cagaggctgc gttggaggcg
    11521 acgcgctcta tgcctttgac cgaaatgcag atgtttgcca cggggctgtc tattcaggcg
    11581 caaacaggtt ccagcttgtc agaggttttg actaatctat cgaatgtaat ccgtgcgcgg
    11641 tttcgtttga agcgtaagat taaggcgctg tcctcagaag ctattgcgtc tgcctctatt
    11701 attgcggcgt tgccggtttt ggtggcgact ggcatgtatt ttgtgaatta cgaatatatt
    11761 agcgtgttgt tcactgatcc gtttggcaat gtgttgctgg cgattgcggt tttctggatg
    11821 agtgtgggtg ttatggtgat gaaaattatg attaacttta aagtttgaat tctaagggcg
    11881 gcgaaaatgt tcatttttaa aacttccggt tctcacatat caaaacatat gctgcgatcc
    11941 gggtttttaa aaacaaacat tttcgccgcc cttagaattc aaacttaatt agaaggtgta
    12001 tagatggata atgaacttat tataacattg gtgagcgcgc ttcttgctgc cgggtctttt
    12061 gcagcatttg cactgccatt tttaaataag acagagaaga aggaacatta tagatcggtg
    12121 gttgataagc gccgtaagga tctttttcag gcgacgcgtg atggcagcat tcataaggca
    12181 aaaacggaaa agaatgtttc ggcggctgat tccatggcag cgctgtataa gatgcagcaa
    12241 atcgcgggtg ctatgggtga aaaagtgcgt gacaaaatgc tgcaggcagg tattcgcagt
    12301 cccaaggcgc cgattaagtt tatgattgcg caagcggttg tgccaattgt tcttgccggt
    12361 ttgacggttt tggttctttc aaatactgag aaagaaattt ctgatatgat gaaacttttg
    12421 tctgttttta cagcggctgt gattggttat aaactgccgg atgtgttgat taagaatacg
    12481 attttgaagc gtcaggagga gatcggtttg gcgtttccag attctttgga catgatgctt
    12541 atttgtgtgc agggcgggat tggtttggag cagactgttg atcgtgttgc agaagaagtg
    12601 gcggagcatt cgccggtttt ggctgaagag ttaggcctgc ttagtgcgga gatggcgatg
    12661 ttgaatgatc ggcgcgaggc gttgcaggat tttgcgcggc gtgtgggtag cggtgcagca
    12721 aaaagctttg caacttcgct tattcaggcg gagcaatatg gaacatctat ttcacaagct
    12781 atgcgcgtta tgtcggatga attgcgtgat atgcgtatgg cgaaggcaga gcaaaaagct
    12841 gcagcattac cgccgaaatt gacagtgccg atgatcctgt tttttttacc ggccttgttc
    12901 attattattc tcggtccggc ggggatttca gcgagctctt tgaattaatg attgaaattt
    12961 gtgcgtgact gaacgcacaa aagaaccgcg gttcctgttc atggctccgc ggcttttttg
    13021 tatcagtttt ttttaaggag aattaatccg tggttgttat tggctttttg cctggtttgg
    13081 gggcaggggt cccgtctgct tgttgcagcg ctgtgataat tcgtaagttg cgttcgattt
    13141 cgacgcgacc aggcgaaacg tcttgagctt tgcgtagaat ttctatggat tcattgagat
    13201 agccttgtga ggccaggttt agcgcgaggt tgttcatgat ggttgtcggg tcgccttgcc
    13261 agagttctat gcctttgcga taggcgcgtt cggcttcctg gtgcatgcct tgcgtgtcca
    13321 gggcgatgcc gagacggtga taagccttga agttggtttc atcggcgatg acggcttttt
    13381 gcgcgtattt ttcagcggct tcgtaattcc cctgtgctaa ttgaatggct gcatattcag
    13441 aggtggccag agaggaggcg ttttttcctt tagcaaaagg ttcgagtatc gttgcggcaa
    13501 gctcgtattt gctattttcg cgcaggccta cggcgtaatc gactgcggca agttcgctat
    13561 caggttgttt tttgtaggtg cgttcgaggt aactaaggga tttacttggt ttgcgtgtat
    13621 agtttgccgc gcgctcaatt gcaccatcca tcgaggatgt gcggagctgc gcatcggtac
    13681 tttgcgctgt ttgacaagcg gtgagtgcac tgcataatgc taatgtcagg atcagggagg
    13741 tggttttttt tatgctcatt gtattatttt cgccttattg aattttgggc tatctgcccg
    13801 cgaccattat acacaaatgt tttgaaagtc ttaagtgtca ttattcggta tcattaattt
    13861 cagcaggagg gcttgtgaga gcgtctgctt cgttggtatt tgtattgcca ccttctacca
    13921 gagctcctgc gtttgagtct gcttcttcac ttcccattat gatctcgtgg cacatgtcag
    13981 gacagtagaa aacgctggtt gattggcaag tatcatcatc ggagcctgcg caggagcgac
    14041 gtatgcgcat gtatttttct tttgggcttg cgacgatgac atggcgttgc atgactatgg
    14101 caccgttttt atccaaaacg gtaaattggg ttgctccggg cgcgcgcgct actaatacaa
    14161 gtgtttggga tgtgtcagcg aggatactta aatgatttgg gttgccgacg atgatggagg
    14221 cggcagggcg atcaaggtgc accaattcag atttatcggg cgtcagcttt atggccgggt
    14281 gggtgtgagt ttggtcgtcg tagataattc cgcgttcttg cgctgttgta gcaggaagga
    14341 tatccaaagg tggctgactg ccattttcct gcgcaaagga cgggacattg acacttaggc
    14401 tctggagcaa aactatggcg ctgaaaagcg cgaatttaag atattttgaa aaaatcatgt
    14461 gaaaaaccct tgataaggga gaaggactat atttttcagt atagccggaa agggcttaag
    14521 aatcaccttc tttttgcatg cattttactt aagttttaaa tgcgcgggtt ttaggagaat
    14581 atatgtttcg tgtgtttcag ttttttaaca ctatgtaaaa ttatggcgct aaaaaaatgc
    14641 aagcttttat tccgggtctg tttagcattg ttgggtaaac tataaacctg tgcggcctgg
    14701 ctatcaactt ttgcgatgac catggtggga aaggcttata gggcgccaag ttttggtcca
    14761 tcggcggctt ttgcgtattg aattggcggt tcggattgtg gagagaagct gataccgtag
    14821 gcgttgttgc ctgccgtgat tgagtacatg gcttccggat tttgcatggc ttcgtgtgag
    14881 aagtgaagat ttaggtttac gatgttgctt gttcggttcg gcgaggcgat ggcgtcgccc
    14941 ggttcagtaa ttcccagatt ggctgccttt ccatcataat agaagttcat ttccgcattg
    15001 ccgggcaaat ttattgtaaa ggtgtcgctg ctcttgattt ggctagggtc gagtacgcat
    15061 ttgatgtcta tccctgtttc ggttgaagcg gcgttgcaaa gcaccgggat gttcgtgttt
    15121 gcaggtaatt tcggcatggg aagttcttgc ggtgtgggtt tgccgccggc cagaagtgtt
    15181 tttggttcgt taggggcgct tgtgcccata atgttgtcta cgccccagag gcgttcgaca
    15241 acggccaggt cttttgtggc gtgttcgact atgcgtgcat tgctggtttc ctgggcaaga
    15301 cgatagagtt cgacggcgag ttttcggtct tgcggtgagc cgttgccttc ataaagcagt
    15361 gaggaggcca gttcattgag cgcccagccg tggccttttt cggcgaattg caggacatat
    15421 tggccgcgct ggccgaaatt ctcagggttg agcgcggcga ggcgttcgag gggggaggcg
    15481 ggagcctctg ccgtgacgat gtgttctttc agggcctggg cttttggttt ttctgccacc
    15541 tctgacgtcg ttggttcaaa ttcaggtgct tcagcgatag ttatttcttc ctgcgcgaag
    15601 gcttcaggcc agagattttt aattgtgtcc agatcgcgcg aggcttggtt tgcgattttc
    15661 gggttgtcgc tgtgttctgc gatttgcagc agttcgacgg caaattcacg gttgagcggc
    15721 gagccgttat tgccgcttaa gaggtgaatg gccatttcct tgacggccca ggcatgccct
    15781 tttttcgcgt aggataaggt ttcggctgcg cggcctgaga aattatcggg gttaatttga
    15841 ctcagttttt ccagcggggt gagtgccggg gtggcggcga ttttctgagg ttcggtctga
    15901 gttacggcag tttgcggctc tgttgcttct gttgcaggca tttctgccgg aggagtggtt
    15961 attccctgtt ctggtggttg ataatgattg gcaatatttt ccggcattat caggtcgtct
    16021 atctctccgg cgtttgcggc gtgggcgaac gtttcaacca gtccggcagc gccgattatg
    16081 cccaggccag agcttatggc tagttgtttc gcatactttg ccttgttctg gccgagaaat
    16141 gagaaaaatc ctttcaggtt tgcccaaaag ccgtgtgggc ggtctttatt ttgcgtgcgg
    16201 tagtcttttg cggccttgta ggttgtgacg gcagcgccgc ccaatactgc tgcggcaact
    16261 gcgccggagc ccgctgttga agccaggctg atggcggctg ttttcaccgc ataggccgcg
    16321 ccagcgccca tggcggcttt ggcaaaggtt ttggtcagcg ggttgtcggt gattttgtgg
    16381 atgcggtttt gcagagcggt tttggcgcgc tcggccacgg taccggcgcg ttgccaatga
    16441 tcgcgcaagg agggggctgg tgtataaagc tgtgcagcgg cggttgcgct catatttcaa
    16501 tatcccaaat gagaggggtg ttatagcgcc ggtttttaaa ttatgcaaat aaaatgtttt
    16561 acaaaatatc ttgacataag tttcaggtta tggaattaaa tgcgcattct ttacagaatt
    16621 ctttggattt tttaactcga ccaatgaggt gatgagatga gtaatgtgac aaatagcaga
    16681 gttattgcag ggacccttgt ggttgcgatg tcaggtgcgg cgcaggccgg tggttatggt
    16741 ggcggtgatg agattggtga acagaataat tattatggcg atgattatga gttgatcatg
    16801 aatggtggtt ctcccagtgc ggctgcgacg gccggtcttg aaatggaagg aagtttgaat
    16861 ccttctgctg cagcgaacaa tcatgcgaac gtgacgggcc gtgtggatag ccggaatact
    16921 ttgaccggaa acaatagtct tgaagttgag cgtattggtt cagattcacg agcaaatgtt
    16981 aatattgcac ctggggccat tcaacctgtt gctaatggcg gcgctgcaga gcaagaacag
    17041 acgaacggga ataatgaaat tcaagttgat gatcattcga tcaataaaat tgatgcgcgt
    17101 cagggtgcgg caacgaatgg tatggcagcg cgccttgaaa cggcaaggtt cctggctcaa
    17161 gcttgtgaat ctcgtttttc tgttgaggga cggcttggtg atgtttttgg taattatgct
    17221 ggcgcgggca tttcctatca ccccagcctt aagtttgtaa ttcaaaataa aaaaacgggc
    17281 gctattttta gtgggcaaca gctttgggat gctgctcccg tagatcgtgg ttatatgacg
    17341 tatggtatgg acgaagacac tattgcgttg gcaatgtgtt ttgctgaagc gctttatgga
    17401 actggagata gagtgcacag acacattgtt acagaagaag cgcaacgcca taattgggaa
    17461 cgttcaaaga atttgccagg tgtaaaagat gaagaaaaag cttctgtagc agaacatatg
    17521 caagctcaca tgtatgttat gggggctaat tctgtatatc gtgatgctat gggatgtacg
    17581 atgacatcat atgttaacga aggcgggaca ggtgagatta atcaaaaaga tcgcccaacg
    17641 ccgacgcagg ttatgatgaa tatgtttggt cttaaaaaac cgactaaaga tcagagtaca
    17701 ggtagagtgg ctcaacagcg cgccaagctt attcctgaat gtgagtttaa gacaactatt
    17761 cctgaacctc cgaagaacga aggtgctggt aatggcaagg tctctgatga atctggaacg
    17821 aacaatattt ttgatgttct ttctccgaac taggaaacgg cgtttatttt tcaatggtcg
    17881 tttaaaagac cccgctgcta tcttggtggc gggttctttt tttattgact tttatttaca
    17941 taatttgtac aagtttattc tgatttttag gattgtatta tggcagcgtt caaacatatg
    18001 gctgattttt catctttcgc aaacgataat tttgtgcgtg gatatggagc acgcgctctg
    18061 agggtgcttg cacctgtgag tttagcggtg cttttaagtg cttgcgcttc gactaaagag
    18121 gttatggagg aaagtacaac gccaaactgt gtagaccagc agacttatac caagtttatt
    18181 agcaaaactg tgaaagagga tgttgaagtt tcttcgaagg atgggagtag taaagtagag
    18241 cagcgtgaaa aaacggttga taagatcgtt gagcgtttca gaattaataa ggggttcaat
    18301 gagcaatgtg aggcccacaa gattttggtt cttacggccg aggcgcaaca ggacaacgaa
    18361 gttgggatta ttgcaagcat ggctttgttg tttttggctt ccggtcagga tgaagcgacg
    18421 caccaggcta ttactgagat gttgcagcgt tacgatagca gtttagaaaa gctgacggtt
    18481 cgcgttgatg aatttgataa aaagcgtgcg attgatttca tcgatacgca ggtgggggat
    18541 ttgtatcatg gtaatggcca gccaaattat ccggcggtag cggcgatatt ggggctgtat
    18601 gatcaggatt ctcagccgga gggcaaacgg cgctatccaa agctggagca ggcgtttttg
    18661 cgtgaggtga ttgacggggc gctggttgcg cgggggcttg atattgcaga gcttaagcaa
    18721 atgtatgagg gggcgcggcg taaaataggg tgcacgagtg aaaaaattgg cagatatatg
    18781 cagacacgtt gtgaagtgca agaggatctg ccggtttacg ggccttaaga cgggctcatg
    18841 gcgctgagag gtggtcccaa gaattcggac agtgagttaa gctacgatct atgaccga
//
LOCUS       DAOSWR010000023        18118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1968_length_18118_cov_5.106571, whole genome shotgun sequence.
ACCESSION   DAOSWR010000023 DAOSWR010000000
VERSION     DAOSWR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18118
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_18118_cov_5.106571"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="tolB"
                     /locus_tag="PK513_02995"
     CDS             complement(<1..777)
                     /gene="tolB"
                     /locus_tag="PK513_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102732.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HOO81453.1"
                     /translation="MKKILFALLLLLVMPQAAHAELVLDLKRGVVEPMPIAVSSFHAA
                     SQQHHDFATQIPGVISANLESSGLFKPLNPRAFVQDVNSMQIAGPRFGEWRAIGTQAL
                     VSGSVTTTADGRTRVEFRLWDVFSEKQLIGMAYMTTPENWRRIAHIISDEIYKRLTGE
                     EGYFDTRIVYVAESGPPQARVKRLAIMDQDGANHKYLTDGRDLVLTPRFSPSRQQITY
                     MSYGRRGPRVYLYDINTGRHEELGNFPNMTFAPRFSPDGNK"
     gene            complement(804..1661)
                     /locus_tag="PK513_03000"
     CDS             complement(804..1661)
                     /locus_tag="PK513_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOO81454.1"
                     /translation="MNAARPDNLQSAHQGSMKGALIKSSIFHGLVITIALVGIPFVKT
                     EPLIVAPVSVELVDISELSQTDQKPSPKPKPIPTPEEPKKIEPPKPPEEELKKPEAQP
                     EPKKPDPVPLPKPEESKKQPEKPKPKPKEKPKPKPKKEEPKQDDLFNSLLKDLTPTEE
                     EDQNKQNSKNPDLEDGQTAPIANQISMSELDALRRQIEPCWNVPSGAKYAENLVVTLR
                     LSMNRDMTVRDVRVINGGLNPDPAFRAAADSAVRAVRNPRCSPFQLPPEKYEQWKTIT
                     INFDPSDML"
     gene            complement(1667..2113)
                     /gene="tolR"
                     /locus_tag="PK513_03005"
     CDS             complement(1667..2113)
                     /gene="tolR"
                     /locus_tag="PK513_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOO81455.1"
                     /translation="MGASLTTKTSSRGRRTGGTYRPMAEINVTPFVDVMLVLLIVFMV
                     AAPLLTSGVAVDLPQSEAKSIPDEDNKPIEITLAKDGKLYIGDSEMDEKKLKFQLEAM
                     TEGQADRRIYIRADQGLEYGRVMKIIGSINAAGFSKVALISENTNR"
     gene            complement(2113..2883)
                     /gene="tolQ"
                     /locus_tag="PK513_03010"
     CDS             complement(2113..2883)
                     /gene="tolQ"
                     /locus_tag="PK513_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOO81456.1"
                     /translation="MPEAVPAAAVETVENLGSYSHDLSMFGLFMEADLIVKAVMMILM
                     MASLWSWTIIFSKRSNLKRVNRKANIFEDSFWSGEPLDKIYQRVKNSKPDPILATFSA
                     GMEEWQAGVAGGIPAKESLHTSLRQRVERAMNVAIGREINALERGMTFLASVGSTAPF
                     IGLFGTVWGIMNSFSSIAATNNTSLAVVAPGIAEALFATALGLVAAIPAVVAYNVFSS
                     GINSYADRLEAFTDEFMAILSRHLDSQDGEASGRRAAE"
     gene            complement(2898..3299)
                     /gene="ybgC"
                     /locus_tag="PK513_03015"
     CDS             complement(2898..3299)
                     /gene="ybgC"
                     /locus_tag="PK513_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOO81457.1"
                     /translation="MQHTTDIRIYYEDTDAGGVVYHANYIAYGERARAEFLRHIGHQS
                     SNLAKDCGVTFVVKHIDIEYHKPAFLDDLLTVHTSITSIKNSSFTMHHQIKKNGEIIT
                     DLHVVLVCVDANKLKPVRIPEILRAEFEKLI"
     gene            complement(3299..4045)
                     /locus_tag="PK513_03020"
     CDS             complement(3299..4045)
                     /locus_tag="PK513_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81458.1"
                     /translation="MNSKLSEFRSKLNDLNTRLENSGYGALLMGAILIAAFLTLTYYT
                     DFPNFFEKPIPTSMLCLCLLINLLLTIYLIRPKPVRNYIALFITVIVLMFGSYFYGRE
                     YNRINYFISLSGSLFFHWTENEEQYFLWTRANLEEAEVYAEIEEWEAQWAKYSKNFSR
                     MNLGWLYLEGKGTEKNEEKGLELLKQAADEGFFKAHLKLSSYYFEKQDKDNAILYLQK
                     AQETRPDLIPEEYLKTSEESIKNEHSNKKQ"
     gene            complement(4035..5081)
                     /gene="ruvB"
                     /locus_tag="PK513_03025"
     CDS             complement(4035..5081)
                     /gene="ruvB"
                     /locus_tag="PK513_03025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOO81459.1"
                     /translation="MEQAQKNQNLEKDAHEFEKGEEGALRPLSLDEFIGQPALRENLR
                     IFVEASKARGDAMDHVLLFGPPGLGKTTLAQIVSKELGVGFRATSGPVIAKSGDLAAI
                     LTNLEPNDVLFIDEIHRLNPAVEEILYPAMEDGKLDLIIGEGPAARSVQIDLVPFTLV
                     GATTRSGLLTNPLRDRFGIPLRLEFYSPEELSLIVARTARLLNMPLAEDGALEIAKRS
                     RGTPRIAGRLTRRVRDFAHGQNAAKINKAHVDHALGRLDVDASGLDSMDRRYLNCIAV
                     NYGGGPVGVDTLAAALSEQRDMIEDVIEPYLLQRGLLQRTPRGRMISSKGYNYLGLKN
                     KVRSQLSMESLDEQ"
     gene            complement(5183..5791)
                     /gene="ruvA"
                     /locus_tag="PK513_03030"
     CDS             complement(5183..5791)
                     /gene="ruvA"
                     /locus_tag="PK513_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOO81460.1"
                     /translation="MIGKLSGIIDSFSSGHLILDVGGVGYLVHASARTLEKIGQKGDP
                     ASLLIETAVREDAITLYGFVDSAEQSWFKLLTSVQGVGAKAGLAILSALTPEKISLAI
                     AAQDKAMLTQADGVGPKLATRILTELKDKAVNLDLSPKSVEGFVDNSEKTPEGVDNDA
                     VSALINLGYARADAYSAVIRAKDKANDNLQDLIRLALKELSA"
     gene            complement(5856..6344)
                     /gene="ruvC"
                     /locus_tag="PK513_03035"
     CDS             complement(5856..6344)
                     /gene="ruvC"
                     /locus_tag="PK513_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOO81461.1"
                     /translation="MIILGIDPGLQKTGWGLIESLGSALQFKASGLIKTDAAQSLPGR
                     LASLHCELCKIIEAWNPCAAAIEETFVNKNPASALKLGQARGVLLAVPALYGIETAEY
                     SANKVKKSIVGTGHAAKEQMGMMVKTLLPACGQVSEDEADALAVAITHAHYGGVHKAL
                     RA"
     gene            complement(6386..6829)
                     /locus_tag="PK513_03040"
     CDS             complement(6386..6829)
                     /locus_tag="PK513_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81462.1"
                     /translation="MGWMLLTFPVAMKAREFSEISLFMFVCAVAIFTGFLGLNLHYMK
                     VKNAPLIIPMISLGILGFLLFYSFGIVAIFTAEGLGAFGWLLISGFLSFFGGIPVYDG
                     FRIIKKQKSNLKPLDKMSTEERKAFREQSAKEEKEALKSILNDEE"
     gene            complement(6904..7659)
                     /locus_tag="PK513_03045"
     CDS             complement(6904..7659)
                     /locus_tag="PK513_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO81463.1"
                     /translation="MAGHSKWANIKHRKGRADAVRAKLFSKLGREIYVAAKLGGGDPD
                     MNPRLRLAIATARGQSMPKDGIEKNIQKGIGGGEGEEYEEIRYEGYGPGGIAVIVECL
                     SDNRNRTAAEVRSIFAKGNGNLGETGSVNFMFDRCGEIAYPADKAGADDMFEAALEAG
                     ASDVESDDEEHRILCEADDFAAVRDTLEAKYGEPERQGLIWKPNVTSEVDEDGAHAIL
                     RLVDALEDSDDVQHVTTNFEAPDAVMEKIMAEG"
     gene            7831..8355
                     /locus_tag="PK513_03050"
     CDS             7831..8355
                     /locus_tag="PK513_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81464.1"
                     /translation="MAYDFSDVSVLIVEDNLPMVELIKSLLTTFGIKKISSARDGEEG
                     FVLYRKETFDLVITDWMMKPVDGISLTRRLRNDPLSPNQYVPVILMTGFSEKRRVMQA
                     RDVGVTEFLVKPFNARDLYRRIAQIIEKPRQFVRSEDFFGPDRRRKNDRGYSGRERRA
                     EPVMINVRSKMGEA"
     gene            8352..8873
                     /locus_tag="PK513_03055"
     CDS             8352..8873
                     /locus_tag="PK513_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81465.1"
                     /translation="MTHYNQPRRRKAEFIKPTNTLKQKVGHGGLSDEILDKAQKLLEE
                     NTHDFEPLAMMYLATLMQGVEMAKGYSPSTDKEQIIANMLYPTMQLKANGGMFHYNLV
                     TMIADKLIQFLEVIDQPDIEVLEIVLAFHTTIIAILQGKISGDGGKQGQDLLNALSDA
                     CMRYFDKHPDDKL"
     gene            complement(8870..9685)
                     /locus_tag="PK513_03060"
     CDS             complement(8870..9685)
                     /locus_tag="PK513_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOO81466.1"
                     /translation="MRIIFIGDIMGRSGREALEKHLPHLREELKPDIIIVNGENATNG
                     RGINIKACKQFYDWGVDCITTGNHIWDQREIISHIAKDEKLLRPLNFPPGTPGSGVYK
                     HRLNDGRTITIVNAMARLFMDAIDDPFQAINTLLSEEKLGRTTDAILVDFHGEATSEK
                     LSFAHYIDGKVTAVVGTHTHIPTADAHILENGTAYQSDAGMTGDYNSVIGVKKEMAMQ
                     RFIKKMPGERFLPASGEATLCGCLIVSNDKTGKAVSIDPIRLGGILPGIMPTA"
     gene            complement(9771..10334)
                     /locus_tag="PK513_03065"
     CDS             complement(9771..10334)
                     /locus_tag="PK513_03065"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOO81467.1"
                     /translation="MTDQKTILRDQARKHRTLTDIRAEDPDAATPLFFDALQPHAGQI
                     IAAYWPKGREFDPTGILERALNEGLTCALPKVEADTKILRFIQWTHKTKLVKGAFGIL
                     EPEEGPELQPDIFLVPMLAFDRKGYRLGQGGGYYDATLEHYRGKKDVAAVGIAYAAQA
                     VLFNLPAEDHDQPMDWILTPKGIAKYN"
     gene            complement(10446..10624)
                     /gene="ssrS"
                     /locus_tag="PK513_03070"
     ncRNA           complement(10446..10624)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK513_03070"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(10735..11058)
                     /locus_tag="PK513_03075"
     CDS             complement(10735..11058)
                     /locus_tag="PK513_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81468.1"
                     /translation="MRRDGLSEENSNNITIFQRIAETLRKTEQDRINLVTNAPQYIKG
                     EVTLIDNQKAPMGGSPALKINGDSLVLHEFRGRQWIEERQGRGDSSISQILTQMESHV
                     EPQNP"
     gene            complement(11222..11557)
                     /locus_tag="PK513_03080"
     CDS             complement(11222..11557)
                     /locus_tag="PK513_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOO81469.1"
                     /translation="MAEVNITINGRSFGISCEDGQEQRVLDLSHYVDSRMKDISKAGA
                     ASSEAHLMVLTTLLLADEVFDLRNDVGSMSENLQSAQANQNEEAAVAGAIEDLASRID
                     RIASRIQNA"
     gene            complement(11557..11784)
                     /locus_tag="PK513_03085"
     CDS             complement(11557..11784)
                     /locus_tag="PK513_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81470.1"
                     /translation="MSQILEALVKLEQSIGGLEGSVSHIEASLSGQQRDMFSASRPVA
                     ANGNGVDTQTVVKKLDNIIEQAETVLKESHG"
     gene            11973..13997
                     /gene="tkt"
                     /locus_tag="PK513_03090"
     CDS             11973..13997
                     /gene="tkt"
                     /locus_tag="PK513_03090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO81471.1"
                     /translation="MTSKTAAAPDAQIQDLTEPQLKSMANAIRALSMDAVQKANSGHP
                     GAPMGLADVATVLYSKFLKFDPKNPEWPDRDRFVLSGGHASMLLYSLNYLTGYEKMTL
                     EQIKNFRQMGAITAGHPEVEQDAGIETTTGPLGQGIATAVGMALAERILNGRYGDDLV
                     DHYTYVMCGDGDLMEGISHEACSLAGHQKLEKLIVLYDDNNICIDGTVDLTFIDDTPK
                     RFEAYGWDVQTVDGHDFVEIEAAIAKAKTTDTPSLICCKTHIGFGAPTKQDSNKAHGA
                     LLGEDEIKGAREKLDWPHAPFEVPDKILANWRAIGAKGKALSHDWKNRLNSHEQKDAF
                     MGAMTLDLAPALAPLIAKLKSDFATEKPKKATRQLSGMVLEALVPAIDSLVGGSADLT
                     GSNNTKVAASKVINKEDYSGNYINYGVREFGMAACMNGLTLHGGFVPYAGTFLQFADY
                     SRAAIRLGALMKQRVIHVMTHDSIGLGEDGPTHQPVEHVAALRAIPNVYVFRPCDGIE
                     TAESWELAINKKDAPSVLALTRQSLPTLCEYRDENMVARGAYILRDCDGEPEVTIFAS
                     GSEVMLAVEAAEKISAGVRVVSVPCMDLFYENAQKDSADFVKLVCNKSIKIGVEAGIR
                     QGWDRIIGAHSYFIGMDSFGASAPADELYEHFGITTEAIVEAANTKLAQK"
     gene            14012..15025
                     /gene="gap"
                     /locus_tag="PK513_03095"
     CDS             14012..15025
                     /gene="gap"
                     /locus_tag="PK513_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOO81472.1"
                     /translation="MTLRIGINGFGRIGRLVARAVFEGYAGDDVEIVAINDPGGNFFP
                     LLKYDSIHGRAPFTVERDLEHDHIMIDGKVIHRFRSRDPKELKWGDEGVDILLECTGR
                     FKDREGAQLHMDRGAKKVLISAPGEDEDITVVYGVNHDLVQKEHKIVSNASCTTNCLA
                     PVAYALHKEIGIEKGFMTTIHAYTGDQNLVDGSHKDPLRARAAAMSIIPSSTGAAKAV
                     GKVLPELDGKLDGVALRVPTANVSLVDLTFLPSKATSVEAVNAAVTKYAHGEMKGVLD
                     VYDDPCVSIDFNHDPHSSIFSLAGTKIVDGDFVRVMSWYDNEWGFSCRMIDTALKMHK
                     VGY"
     gene            15062..15475
                     /locus_tag="PK513_03100"
     CDS             15062..15475
                     /locus_tag="PK513_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81473.1"
                     /translation="MAENSQSRWGDLLRGYLAGLLFSPFVSIVVVTLFCIFADDYYSY
                     YVNRGDTTFLFLAIEFIAGVALMPLFVALITLAFPLLLFFKIGLYLLYLPFLAIPILL
                     FLPKSYKSYPVLVPALGLFLAFFFVSLTIFLGANS"
     gene            15785..16324
                     /locus_tag="PK513_03105"
     CDS             15785..16324
                     /locus_tag="PK513_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO81474.1"
                     /translation="MTTEGQKACMIIDHLVEALGNDASSSLRRAMILSDIDQNPGTTQ
                     AGIMERLHLDKSTVKREADWLFDYGCIRVQDCDSDGRAKRIEICGYSKKGLDGALGYF
                     DGRHESLKIFLEGLTGYLKQEKPSLRDAKIIAALTEKKAASKQDIIDMLYEGSVATKN
                     RAFTKLLEERLIEEDGEHV"
     gene            16308..16496
                     /locus_tag="PK513_03110"
     CDS             16308..16496
                     /locus_tag="PK513_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81475.1"
                     /translation="MANTFRANAELADGVLALVRRTTGDNVYHLAASNTPHISSGFGR
                     RAGSAPTSMSFVPEALER"
     gene            16587..17501
                     /locus_tag="PK513_03115"
     CDS             16587..17501
                     /locus_tag="PK513_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOO81476.1"
                     /translation="MSNAEQRTKITSGKGFIAALDQSGGSTPKALKNYGVDESAYSNE
                     TEMFARVHEMRTRIITSPAFNGEKILGAILFEKTMDSDIDGTPTAQYLWEELGVVPFL
                     KVDKGLAEQADGVQLMKPMPELDGLLERATAKGIFGTKMRSVIHSASESGIAAVIAQQ
                     FEVGKQILSHGLVPIIEPEVNINADDKADCERIMKAEILKQLDALSDDQKIMLKLTLP
                     EEANFFAELISHPNVLQVVALSGGYDLAEACRRLAENDGMIASFSRALSNDLRAQQSD
                     EEFNAALSNAIDKIYTASVGKEAGAQAA"
     gene            17503..17811
                     /locus_tag="PK513_03120"
     CDS             17503..17811
                     /locus_tag="PK513_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81477.1"
                     /translation="MDLISALFPTLFAKTFFILGTQLFVTWLAAKATLSKFRQLHDWG
                     TPWITSTTNENRHRDLHVDWKHVATPFYALLIVNFAAFLLLLFWGVKQPLSISFGLFD
                     "
     gene            complement(17990..>18118)
                     /locus_tag="PK513_03125"
     CDS             complement(17990..>18118)
                     /locus_tag="PK513_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HOO81478.1"
                     /translation="EDFLAALDHGMPESAGIALGVDRLIMLATGAKEIVQVLWTQD"
BASE COUNT         4311 a         4092 c         4857 g         4858 t
ORIGIN      
        1 tttgttgcca tcaggcgaaa agcgcggggc aaaggtcatg ttggggaaat tgcccagctc
       61 ttcgtggcgg ccggtattga tgtcgtagag gtaaacacgc gggcctcggc ggccatagga
      121 catgtaggtg atctgttggc gcgatggtga aaagcgcggt gtaagcacca ggtcgcggcc
      181 atcggtaaga tatttatggt tggcgccgtc ctgatccata atggcgaggc gtttgacgcg
      241 ggcctgcggc gggccacttt cggcaacata gacgatgcgg gtgtcaaaat agccctcctc
      301 accagtcagg cgtttatata tctcatcaga aataatatgg gcaatgcggc gccagttttc
      361 cggcgtggtc atataggcca tgccgatcaa ttgtttttca ctgaatacat cccagaggcg
      421 aaattcaacg cgcgtgcgtc cgtccgcagt ggtggtaacg ctgccggaaa caagggcctg
      481 tgtgccgatg gcgcgccatt cgccaaagcg cgggccagcg atttgcatag aattcacatc
      541 ctgcacgaaa gcgcgaggat taaggggttt gaacaggccg gagctttcca aattggccga
      601 gatgacgccg gggatttgcg tggcaaaatc gtggtgttgc tggctggccg cgtgaaagct
      661 ggaaacggcg ataggcatcg gttcgacaac gccgcgttta aggtctagca ccagttcggc
      721 gtgtgcggct tgtggcatga ccagcaataa aagaagagcg aagagaatct ttttcatggg
      781 atacatcctt tcgaattcac acgttatagc atatcgctgg ggtcgaaatt aatagtaatc
      841 gtcttccatt gctcatattt ttcgggtggc aactggaaag gcgagcagcg cgggttgcgc
      901 acggcgcgca cagccgaatc cgctgctgcg cggaaggccg gatcaggatt taagccgcca
      961 ttgattacac gtacgtcgcg cactgtcata tcgcgattca tgctcaaacg cagtgtaacc
     1021 actagatttt ccgcatattt tgcgcctgat ggcacattcc agcacggctc aatctggcgg
     1081 cgcagcgcat caagctcgct catactgatc tggtttgcta tcggcgcggt ttgaccatct
     1141 tccagatcag ggttttttga attctgttta ttttggtcct cttcttctgt gggtgtgagg
     1201 tccttgagca gtgagttaaa aaggtcgtcc tgctttggct cttctttttt cggtttaggc
     1261 tttggctttt ccttcggctt tggctttggt ttttccggct gttttttcga ttcttccggt
     1321 ttgggtaaag gcacgggatc cggctttttc ggctctggct gggcttccgg tttcttcagc
     1381 tcttcttccg gcggttttgg cggttcgatt tttttcggct cttcaggcgt cggaatcggt
     1441 ttaggctttg gcgacggttt ttgatccgtc tgggaaagtt cggaaatatc gaccagttcg
     1501 acgctgaccg gcgctacaat cagcggctcc gtttttacaa acggaatacc gacaagtgca
     1561 atagtaatca ccaacccgtg aaaaatcgag gatttgatca gggcgccttt catagagcct
     1621 tgatgggcgc tttgcaggtt atctggacgc gcggcattca tgaaaattac ctgttggtat
     1681 tctcggaaat gagagcgact tttgaaaaac ctgcggcgtt gatagaacca atgattttca
     1741 tcacccgacc atattccagt ccctgatcgg cacggatata aatgcgccga tcggcctgtc
     1801 cttcggtcat tgcttcaagc tgaaatttca gctttttttc atccatttcg ctgtcgccga
     1861 tatagagctt tccgtccttg gccagtgtaa tttcgatagg cttgttatcc tcgtccggga
     1921 ttgattttgc ttcggattgc ggcaagtcaa cggcgacgcc ggaggtcagc aacggggcag
     1981 caaccatgaa aacgatgagc agcacgagca tgacatctac aaagggcgta acgttgattt
     2041 cagccatcgg gcgataggtg ccgccggttc tacggccacg gctgctagtt ttggtggtta
     2101 acgacgcgcc cattattcag ccgcccttct gccggaggct tcgccatctt gcgaatcgag
     2161 gtgccgggaa agaatcgcca taaattcatc ggtaaaggct tcgaggcgat cggcgtaact
     2221 gttaataccg cttgaaaata cgttataggc gacgacagcc gggatggcgg cgaccagtcc
     2281 cagagctgtg gcaaacaggg cttcggcgat gcccggcgca acgactgcaa gcgaggtgtt
     2341 gttggttgca gcgatagaag aaaaggaatt cataatcccc cagaccgtgc cgaacaggcc
     2401 gataaaaggc gcggtcgaac caaccgaagc cagaaaagtc ataccgcgtt caagtgcgtt
     2461 aatctcacga cctatagcga cattcatcgc gcgttcaacg cgctgacgca gtgaggtatg
     2521 cagactttcc ttagcaggga tgccgccggc aacgccggct tgccattcct ccataccggc
     2581 ggaaaaagtc gcaagaatag ggtcaggctt ggaattttta acgcgctgat agattttatc
     2641 gagaggttcg cccgaccaga aagaatcttc gaaaatattg gctttgcggt tgacgcgctt
     2701 aagattggag cgtttggaaa aaatgatggt ccagctccag agcgatgcca tcatgaggat
     2761 catcattacg gctttgacga tgagatcggc ttccataaac aggccgaaca tgctgagatc
     2821 gtggctatag ctgccgagat tttcgacagt ttcaacggcg gcggcgggaa cggcttcggg
     2881 catggtcttt tttcctctta gatcagcttt tcaaattcag cacgtaggat ttcaggtata
     2941 cgcactggtt ttagcttatt tgcatcgacg cagacaagca cgacatgcag atccgtgatg
     3001 atctccccgt tttttttgat ctgatggtgc atggtaaagc tcgaattctt tatgctggta
     3061 atgcttgtgt gaacggtgag caggtcatct aaaaaagccg gtttgtgata ttcaatgtcg
     3121 atatgtttta ctacgaatgt gacaccgcaa tccttcgcaa ggttactgga ttggtggcca
     3181 atatggcgca agaattcagc gcgggcgcgt tcaccatagg cgatgtagtt ggcgtgatag
     3241 acaaccccac ccgcatcggt gtcttcatag taaatgcgga tgtcggttgt gtgctgcatt
     3301 actgtttttt gtttgagtgt tcgttcttaa tagattcttc tgatgttttt aaatactctt
     3361 caggtatcag gtcaggcctt gtctcttgcg ccttttgcaa atataaaatc gcgttatcct
     3421 tatcctgttt ttcaaagtaa taactagata atttaagatg tgccttaaaa aaaccttcat
     3481 ccgcagcctg tttaagaagt tctaagccct tttcttcatt tttttctgtt ccttttccct
     3541 caaggtaaag ccagcctaaa ttcatacgtg aaaaattctt tgaatacttt gcccactgcg
     3601 cttcccattc ctctatttct gcgtaaacct ccgcttcttc caaatttgct ctggtccata
     3661 aaaagtattg ttcttcattt tcggtccagt ggaaaaagag agaaccagat agtgagataa
     3721 aataatttat ccgattatat tcccttccat agaaataaga accaaacatc aaaacgataa
     3781 cggtaataaa aagggctata taatttctga ccggcttcgg cctgatgaga taaatagtta
     3841 gcagcaaatt aattaaaaga cacaggcaca gcatagaggt gggaatgggt ttttcaaaaa
     3901 agttcgggaa atccgtatag taagttagtg ttaagaaggc ggcaattaag atagcgccca
     3961 ttaacaaagc gccataaccg ctattctcaa gacgagtatt gagatcgttt aatttggaac
     4021 gaaattcaga caatttactg ttcatccaaa ctctccatag atagttgaga tctaacttta
     4081 ttttttaacc ctaagtaatt gtatcctttt gaagaaatca ttcttccacg tggagttctt
     4141 tgcagcaggc ctcgctgaag aaggtagggt tcaatgacgt cttcgatcat gtcgcgctgt
     4201 tcggacaaag cggcggcgag cgtatcgacc ccgacggggc cgccgccgta attcacggcg
     4261 atgcaattga gatagcgccg gtccatggaa tcaagaccgc tggcatcgac atcgaggcga
     4321 ccaagcgcat gatcgacgtg ggctttattg attttggcgg cgttttggcc atgagcaaaa
     4381 tcgcggacgc ggcgtgtgag gcgcccggca atacgggggg tgccgcggga gcgcttagcg
     4441 atttcaagcg caccgtcttc ggccaggggc atattcagca ggcgcgccgt gcgggcgacg
     4501 atgagggaga gttcttcggg agaatagaat tcaaggcgca gcgggatgcc aaaacgatcg
     4561 cgcaaggggt tggtgagcag gccggagcgt gtggtggcgc caacaagcgt aaagggcacg
     4621 agatcgatct gaacgctgcg cgcagcgggg ccttcgccga tgatgaggtc gagcttgccg
     4681 tcttccatcg ccgggtagag aatttcctcg accgccgggt tgaggcggtg gatttcatcg
     4741 atgaacagga cgtcgttagg ctcaaggttg gtgagaatgg cggcgagatc gccggatttt
     4801 gcgatgacgg ggccggaggt ggcgcggaaa ccgacgccga gttctttgga aacgatctgc
     4861 gcgagtgtgg ttttgcccag ccccggcggg ccgaacagca ggacatggtc cattgcatcg
     4921 ccgcgggctt tggaggcttc gacgaaaatg cgcaagtttt cgcgcagggc cggctggccg
     4981 atgaattcgt cgagggatag tgggcgcaaa gccccctcct cgcctttttc gaattcgtga
     5041 gcgtcttttt ctaaattctg gtttttctgc gcttgctcca ttaaatcatc ctaaatgtca
     5101 tttcgagcga atgcgagaaa tctcatatgt ttgaaggaga ttcctccttg ctgcgctcgt
     5161 tcggaatgac aatgaaatct gttcatgcgc taagctcctt aagggcgagg cggatgaggt
     5221 cttgcaggtt atcgttggct ttatctttgg cgcggatgac ggcggagtaa gcatcggcgc
     5281 gggcgtagcc gagattgatg agggcggaga cggcgtcgtt gtcgactcct tcgggtgttt
     5341 tctctgaatt atctacaaac ccttccaccg attttggaga taagtctaga tttacggctt
     5401 tatctttaag ttcggtcagg atgcgtgtgg cgagcttggg gccgacacca tcggcttggg
     5461 taagcatggc tttgtcctgc gcggcgatgg cgagggatat tttttccggt gtcaaagccg
     5521 agagaatggc caggcccgct ttggcgccga cgccttggac ggaggtcagg agtttgaacc
     5581 agctttgctc cgctgagtcg acgaaaccat aaagcgtgat ggcgtcctcg cgcacggcgg
     5641 tttcgatcag cagactggcg gggtcgcctt tctggccgat cttctctaac gttcgggcgc
     5701 tggcatggac cagatagccc acgcccccga catccaaaat cagatggccg gaggaaaaag
     5761 aatcaataat gccgcttaat ttaccaatca tagaggatac tcatcacttt gtttttgttc
     5821 aaaaaagttg aaatccttcg ctttgttttt aatttttacg cgcggagggc cttatgaacc
     5881 cctccataat gagcgtgagt gatggcgacg gcaagcgcgt cggcttcgtc ctcgctgact
     5941 tgcccacagg ccgggagcaa ggttttgacc atcatgccca tttgctcttt ggcggcgtgg
     6001 cccgtgccga caatcgattt tttgacctta ttggcggaat attcagccgt ttcaatgccg
     6061 taaagcgcgg gcacggccag aagaacaccg cgcgcctggc cgagtttgag ggcgctggcc
     6121 gggtttttat tgacgaaggt ttcctcgatg gcggcagcgc atgggttcca cgcctctata
     6181 attttacata gttcacaatg tagagaggca agcctgcccg gcagggattg ggccgcatcg
     6241 gttttaatga ggccagaggc tttgaattgc agcgctgaac cgaggctttc tataagcccc
     6301 caaccagttt tttgtaatcc gggatcgatg ccgagaatga tcatgtgtaa atcataccac
     6361 aaaattggaa cgaaacaaga acaaattatt cctcatcatt caaaatagat ttaagagctt
     6421 ctttttcttc ttttgcagat tgctcccgaa atgcttttct ttcttcagtg gacattttat
     6481 ctaaaggttt aaggttgctt ttttgttttt ttataattcg gaagccatca taaacgggga
     6541 taccgccaaa aaaagacagg aagcccgata ttaataacca gccgaaagca cctaagccct
     6601 ctgctgtaaa aatcgcaact ataccaaagc tgtaaaatag tagaaatcct aaaattccga
     6661 ggcttatcat agggataatc agaggggcat tttttacttt catatagtgt aaatttaatc
     6721 ctaaaaatcc ggtaaagatc gctaccgcac acacaaacat aaaaaggctt atctctgaga
     6781 attctcgagc tttcattgca acaggaaatg taagcagcat ccatcccatg cagacgagtg
     6841 cgtaaaggat atagatacga taaatgataa aattaagcct gaagatttcc ttcaatattg
     6901 ccattaccct tccgccatga ttttttccat caccgcgtcg ggggcttcga agttggtcgt
     6961 gacgtgctgg acgtcgtcgc tgtcttcgag ggcatcgaca agccggagaa tggcgtgcgc
     7021 gccgtcttcg tccacttcgc tggtgacgtt gggtttccag atgagcccct ggcgttcggg
     7081 ttcgccgtat ttggcttcga gcgtatcgcg cacggcagca aaatcatcgg cttcgcataa
     7141 tatccggtgt tcttcgtcgt cgctttcaac atccgaggcc ccggcttcca gcgcggcttc
     7201 gaacatatca tcagcgccgg ctttatccgc aggataggcg atttcgccac agcggtcgaa
     7261 catgaaattc accgaaccgg tttcgccgag attgccattg cctttggcaa agatcgagcg
     7321 tacttcggcg gctgtacggt tgcggttatc gcttaaacat tcaacgatca cggcgatgcc
     7381 gccaggtcca taaccttcat agcggatttc ctcatactcc tcgccctcgc cgccaccgat
     7441 gcctttttga atgttttttt cgatgccgtc tttgggcata gattggccgc gggccgtggc
     7501 gatggccaga cgcaggcgcg gattcatatc cggatcaccg ccgccgagct tggctgcaac
     7561 atagatttca cgccccagtt tagagaagag cttggcacgc acagcatcgg cgcggccttt
     7621 tctgtgctta atatttgccc attttgaatg tccagccatt gatttaccta cttttttccg
     7681 gtttattaga tgaggtaagt ataaaagggt tcttcagcct ttcaagaggt ttttcctttg
     7741 atatttgcat atttttgaat aaaaagttcc agaatttaca attacttgtg ctaaaatgct
     7801 aagagggata aaagcgagtg gaccagatag atggcctatg atttttcgga tgtttccgtg
     7861 ctgattgttg aagacaacct gccgatggtt gagcttatca aatccttgtt aacaactttc
     7921 ggaatcaaaa aaatatcgag cgcacgtgat ggcgaggaag gttttgtcct ttatcgtaaa
     7981 gagacattcg atctggttat tacggactgg atgatgaagc cggttgacgg catatctttg
     8041 acgcgacggc tacgcaatga tccactcagc cccaatcagt acgtccctgt gattttgatg
     8101 acggggttta gtgaaaagcg gcgggtgatg caggctcgtg acgtgggtgt tacggaattt
     8161 ttggtgaagc cctttaatgc cagggattta tatcgcagaa ttgcgcagat cattgaaaag
     8221 ccgcgtcagt ttgtgcgttc ggaagatttt tttggtcctg atcgtcgccg caaaaatgac
     8281 agagggtata gcgggcggga gcggcgcgcc gagcctgtta tgataaatgt acgaagtaaa
     8341 atgggcgaag catgacgcat tacaatcaac cccgacggcg aaaagcggaa ttcattaagc
     8401 cgactaatac tttgaaacaa aaagtcggcc atggcggatt gagcgatgag attcttgata
     8461 aagctcaaaa gcttctggag gaaaatacac atgattttga gcctttagcg atgatgtatt
     8521 tggcaacgct gatgcaaggc gttgaaatgg ccaagggata ttctccatca acggataaag
     8581 agcagattat cgctaacatg ctctatccta cgatgcagct taaggccaat ggcggaatgt
     8641 tccattacaa ccttgttacg atgattgccg ataagcttat tcaattcctt gaggttattg
     8701 atcagcctga tattgaagtg ctggaaatcg tgctcgcctt tcacacaacg attattgcca
     8761 ttcttcaggg aaagatttcc ggcgatggcg gtaagcaggg acaggatttg ctcaatgccc
     8821 tgagcgatgc gtgcatgcgc tattttgaca aacacccgga tgataagctt taggccgtcg
     8881 gcataatgcc ggggaggatg ccgcccaaac ggatagggtc gattgagaca gccttgccgg
     8941 tcttgtcatt ggaaacgatt aagcagccgc aaagcgtggc ttcgccgctg gcgggaagaa
     9001 aacgctcacc gggcatcttc ttgataaaac gctgcatcgc catttctttt ttaacgccga
     9061 tgaccgagtt ataatcgccc gtcatgccag catcgctttg gtaagccgta ccattttcca
     9121 agatatgtgc gtcggcggtg gggatatgag tgtgggtgcc aacgacggcg gtaactttgc
     9181 cgtcgatata gtgggcgaag gaaagttttt ccgatgtcgc ctcaccatga aaatcaacta
     9241 aaattgcatc ggttgtgcgt ccgagttttt cctcagacag cagcgtattg atggcttgga
     9301 acggatcgtc gatagcgtcc atgaacagcc gtgccatagc gttgacgatg gtgatggtgc
     9361 gtccgtcatt cagacgatgt ttataaacac cgctgccggg tgtgccgggc ggaaaattga
     9421 gcgggcgcag gagtttttca tccttggcta tatgagagat tatttcgcgc tggtcccaga
     9481 tatggttgcc ggtggtgatg caatcgactc cccaatcgta gaattgtttg caggctttga
     9541 tattgatccc gcggccattg gtggcatttt caccgttgac gatgatgatg tctggtttca
     9601 gttcttcgcg cagatgtggc aaatgctttt caagagcttc acggccagag cgacccataa
     9661 tatcgccgat aaaaattatt ctcattattt ctccttaatt gtcaccggcg aacacgaagc
     9721 aaaatattat ttttggattg cttcgtcgtt gcactcctcg caatgacagg tcagttatat
     9781 ttcgctatgc ctttgggcgt taagatccag tccattggct ggtcatgatc ttcggcaggc
     9841 aggttaaaca aaacggcttg ggctgcatag gctattccca cggctgcgac atcttttttc
     9901 ccgcggtagt gttcaagtgt tgcatcataa taaccaccgc cctgccctag cctgtaaccc
     9961 ttgcgatcaa aagccagcat agggacaagg aagatatcag gctggagttc cggtccctcc
    10021 tccggctcca atattccaaa cgcgccttta accagttttg ttttatgggt ccactgtatg
    10081 aagcgcaata ttttggtatc tgcttcaacc tttggcagtg cgcaggtcag gccttcattg
    10141 agagcacgct ccaaaattcc ggttgggtca aattcgcgtc ctttgggcca ataggctgcg
    10201 attatctgcc ccgcatgcgg ttgaagcgca tcaaagaata gcggtgtggc cgcgtcagga
    10261 tcttcggcgc gaatatcggt aagtgtacgg tgcttgcggg cctggtctct gaggatggtt
    10321 ttttggtcgg tcatggtgtt attttttcgt cggcatcatt gcgagcgtag cgaagcaatc
    10381 tataagataa aagtaaaaaa tggattgctg cgtcggcttc gcctcctcgc aatgacgagt
    10441 tgcgggtggg cgccgcaaag gccgtgtaca aagcaatatc cgcatgggac cctggtaacc
    10501 aggtgggcgc cgtaataatc aaatccccaa tgcaaagaga gtggataagg tcagggacag
    10561 ctccctatgc gaaaagcatc ggcctccggg ataaatagat gtatcacgca ccccgcagca
    10621 aacccatggg cactatgggg tatttaggga tgacgtttca agagaattcg ttcgtgtatt
    10681 ttttctaaaa aaacgaatta acagactttt cataagttga attccttttc cacattatgg
    10741 gttctgaggc tccacgtggc tctccatttg cgtaagaatt tgagaaatag aagaatctcc
    10801 tcttccttgc ctttcttcaa tccattgcct tcccctaaac tcatgaagaa caagagagtc
    10861 tccgtttatt tttaacgcgg ggcttcctcc catcggcgct ttctggttat caatcagcgt
    10921 tacttctcct tttatgtatt gaggagcgtt tgttaccagg tttattcggt cttgctctgt
    10981 ttttctcagt gtctcagcaa ttcgctgaaa aattgtaata ttattactat tttcttcgct
    11041 caaaccatct ctgcgcattg cttaattccc ttaaagtatc gttttatgac ttgattgtta
    11101 atttttgcac ggtattgcgc tcatatgaaa actgtcaatg attaaattgc gcacaatcaa
    11161 ggatgaaggt agatttttgc ggcgggcttt accctcctca caatgacaaa aagtagaaaa
    11221 gctaagcatt ctggatgcgg ctagcgatgc ggtcgatgcg gctggccaga tcttcaattg
    11281 ccccggcaac ggcagcttct tcgttttgat tggcctgcgc gctttgcaga ttttcgctca
    11341 ttgaaccaac atcgttgcga agatcaaaaa cttcatcggc cagcaaaagc gttgtcagaa
    11401 ccatgaggtg tgcttcgctg cttgccgctc cagccttgga aatatctttc atgcgcgaat
    11461 cgacgtaatg gctgagatcg agaacgcgtt gctcctgtcc gtcttcgcag gaaatgccga
    11521 aagaccgacc attgattgta atatttactt cagccattag ccgtgacttt ctttaaggac
    11581 agtttcggcc tgctcgataa tattatccag ctttttgaca acggtctgag tatcaacgcc
    11641 gttgccattt gcagcgacgg gtcgtgacgc actaaacata tcgcgttgtt gcccggacag
    11701 tgaggcttca atatgactaa ctgagccttc caaaccacca atagattgtt ccagctttac
    11761 cagagcttcg agtatctgag acacggcgca cctttcttgt tctatatgtg aatgggcata
    11821 cgctaacagg gcggctgaat ctccgtcaag atggtgaaaa gttttttccc acttaaacca
    11881 caggaattta tgtgctgtgt gcttgacggg aggcctcctt ggcggcatgt taggcgcaca
    11941 aaattatact tataagtatg gatcactgat tgatgacctc aaaaaccgcc gccgctcctg
    12001 atgcgcaaat tcaggacctc accgaacctc agcttaaatc tatggccaat gccattcgcg
    12061 ccctgtcgat ggatgcggtg caaaaagcca attccgggca cccgggcgcg ccgatgggat
    12121 tggccgatgt ggcgacagtt ctttattcga agttcctgaa atttgatcct aaaaatccgg
    12181 aatggcctga tcgtgaccgg tttgtgcttt caggcgggca tgcgtcgatg ttgctgtatt
    12241 ctcttaatta cctgaccggg tatgagaaga tgacgctaga gcagatcaaa aatttccgtc
    12301 agatgggggc gattacggcc gggcatccgg aagttgaaca ggatgcaggg atcgagacaa
    12361 ccaccgggcc gctggggcaa ggcattgcta cagccgtggg gatggcgctg gctgaacgga
    12421 ttttgaatgg ccgttacggt gatgatcttg ttgatcacta tacatatgtg atgtgcggtg
    12481 atggcgattt gatggaaggg atcagccatg aagcctgttc gctggccggg catcaaaagc
    12541 ttgagaagct gattgtactg tatgatgaca ataatatctg catagatggg acggttgatc
    12601 tgacgtttat tgatgatacg ccgaagcgtt ttgaggctta tggctgggat gtgcagacgg
    12661 ttgacggtca tgatttcgtc gagattgaag cggcaattgc caaggcgaaa accaccgata
    12721 cgccgagtct gatttgctgc aaaacgcata ttgggtttgg cgcgccgacc aagcaggaca
    12781 gcaacaaagc gcatggcgcg cttttgggcg aggatgagat taagggcgcg cgggaaaaac
    12841 tggattggcc gcatgcaccg ttcgaagtgc cggataagat tttggccaac tggcgggcca
    12901 tcggggcaaa aggcaaggcg ctttcccatg actggaagaa tcggttaaac agccatgagc
    12961 agaaagatgc ctttatgggg gcgatgactt tggatctggc ccctgctctc gcgccgctga
    13021 ttgcgaagtt gaaaagtgat tttgcgactg agaagccgaa aaaggcgacg cgccagcttt
    13081 ccggcatggt gctggaagct ttggtgcctg ccattgactc gctggttggc gggtcggcgg
    13141 atttgactgg gtcaaataac accaaggtgg cggcatccaa ggtcatcaat aaagaggatt
    13201 attccggcaa ttatatcaat tacggggtga gggaatttgg catggccgcg tgcatgaacg
    13261 ggctgaccct gcatggcggg tttgttccgt atgccggaac attcctgcaa tttgctgatt
    13321 attcgcgggc cgccattcgt ttgggcgcat tgatgaaaca gcgggttatt catgtaatga
    13381 cccatgattc aatcgggctg ggggaagacg ggccgacgca tcagccggtt gagcatgttg
    13441 cggcgctgcg ggccattccc aatgtgtatg tgttccgccc ttgtgacggg attgaaacgg
    13501 cggaaagctg ggagttggct ataaataaaa aagatgcccc ttctgtgctg gcgttgacgc
    13561 gtcaaagctt gccgactttg tgtgaatacc gcgatgagaa tatggtggcc cgcggggctt
    13621 atattctgcg cgattgcgat ggcgagccgg aggtgactat ttttgcttcg ggttcggaag
    13681 tgatgctggc ggttgaagcc gcagagaaaa tcagcgctgg cgtacgggtt gtgtccgtgc
    13741 cgtgtatgga tttgttttat gagaatgcgc agaaagacag cgcagacttt gtaaagctgg
    13801 tgtgtaacaa gagtatcaag atcggcgttg aagcgggcat tcgccaaggg tgggaccgga
    13861 ttatcggtgc gcattcatat ttcatcggga tggacagttt tggagcctcg gccccggcgg
    13921 atgaattgta tgaacatttc ggcattacaa cggaagcgat tgttgaagcg gctaatacta
    13981 aattggctca aaagtaagga gaataaagct catgacatta cggattggga ttaacgggtt
    14041 tgggcgtatt ggacgtttgg tcgcgcgggc cgtgtttgag ggctatgctg gcgatgacgt
    14101 cgagattgtg gcaatcaatg atccgggtgg aaactttttt ccacttttga aatatgattc
    14161 cattcacgga cgcgcgccat ttaccgttga gcgcgatctt gagcatgatc acattatgat
    14221 tgacggcaag gttattcacc ggttccgcag ccgtgacccg aaggaactga aatggggcga
    14281 tgaaggcgtt gatattttac tggagtgtac ggggcgtttt aaggatcgcg agggcgctca
    14341 gttgcatatg gatcggggtg cgaagaaggt tctgatttct gcgccgggtg aagatgaaga
    14401 tattacggtt gtttacgggg ttaatcacga tcttgtacaa aaagagcaca agattgtttc
    14461 aaatgcgtca tgcacgacaa actgtctggc cccggtggct tatgcgctgc ataaggaaat
    14521 cggaattgaa aaaggtttta tgacgacgat tcatgcctat acgggcgacc agaatctggt
    14581 tgacggttct cacaaagacc ctttgcgtgc gcgggcggcg gcgatgagca ttatcccctc
    14641 ctcgaccggc gcggccaagg ctgtgggcaa ggtgttgccg gagctggacg gtaaattgga
    14701 cggcgttgcg ctgcgcgtgc cgacggcgaa tgtgtcgctg gttgatctga cgttcctgcc
    14761 gtcaaaagca acgagcgttg aggcggtgaa cgcggctgtg acgaaatatg cgcacggtga
    14821 aatgaaaggc gtgctggatg tttatgacga tccgtgtgtg tcgatcgact ttaatcatga
    14881 tccgcattct tcaatcttca gtctggccgg gacaaaaatt gttgatggcg atttcgtccg
    14941 ggtgatgagc tggtatgaca atgagtgggg tttttcctgc cggatgatcg acaccgcgtt
    15001 gaagatgcat aaagtcgggt attaaaattt gtcttttagc acgaaaattc gtgacttgct
    15061 cttggccgaa aactctcagt cacgatgggg agatttgttg agaggctatc ttgcaggctt
    15121 attattcagc ccctttgttt ccattgttgt tgttaccctc ttttgtatct ttgctgatga
    15181 ttattattca tattacgtta acaggggaga taccactttt ctttttcttg ctatagaatt
    15241 tattgcaggc gttgccctga tgcctctttt tgtggcccta atcacattgg cttttcctct
    15301 tttgttattt tttaaaattg gtctctatct tctctatctt ccctttttgg ctatacccat
    15361 attactgttt cttccgaaaa gctataaaag ttaccctgtt cttgttcctg cattggggct
    15421 cttccttgcg ttcttttttg tttctcttac aattttctta ggggctaact cctaaatatt
    15481 gcattaaaaa tgcatgaaac tggctattaa atattactta ttgaataccc ctaaaaaata
    15541 tacctgattt tgttgacagg aggcgaatcg ctcaccatat ttgtgagtct aaaccgttct
    15601 ccgtggcaaa gccggtctgt gaaattcgtt tccaggccgg cttttttatg cacataaacc
    15661 ccgcccaatg ctataaatta ttacacttgt ggcatatata ttaacaaaat gtcaaatttt
    15721 cttgtattag aaacgcctga tggtgtatat aagagttatg gttacacatc tcgcaaagcg
    15781 ggtaatgaca acagagggcc agaaggcctg catgatcatt gatcatcttg tcgaagcctt
    15841 ggggaatgac gcctcctctt ctttgcgccg ggccatgatc cttagcgata ttgatcaaaa
    15901 cccggggacc acgcaggccg gaattatgga gcgtttgcat ctcgataaat ccaccgtgaa
    15961 gcgcgaggca gactggcttt ttgattatgg ctgcattcgg gttcaagatt gtgacagcga
    16021 tggccgcgcc aaaaggatag agatttgcgg ttattcgaaa aaaggcctgg acggcgcact
    16081 gggatatttt gacgggcggc acgagagttt gaagattttc ctggaagggc ttacgggata
    16141 tttgaagcag gaaaaaccct cgcttcgcga tgccaagatt atcgcagcgt tgacagagaa
    16201 aaaggctgct tcgaagcagg atattataga tatgctttat gaagggtcag tagcaaccaa
    16261 gaacagggcg tttaccaagt tactggaaga aaggttgata gaagaagatg gcgaacacgt
    16321 ttagagcaaa tgcagaactg gctgatggtg tgctagcgct tgtcagacgc acaacaggcg
    16381 acaatgttta tcatctggca gcttcgaaca cgccccatat ttccagtggt tttggccgtc
    16441 gtgccggtag cgcaccgaca tcaatgagtt ttgtccccga ggctcttgag cgctaggcgt
    16501 aatcccccct tgttaaacat cagaaggttg gctatacgta agggcaatag ccaatttttt
    16561 gtttttaatt cgatggggtg ctcttcatgt caaatgctga acaacgtaca aaaatcacat
    16621 caggaaaagg gtttatcgct gctctggatc aaagcggtgg gtcgacaccc aaggcgctga
    16681 agaattatgg tgtggatgaa agcgcttata gcaatgagac ggaaatgttt gctagggtgc
    16741 atgaaatgcg tacgcggatt attacctctc cggcgtttaa cggcgagaaa attttgggcg
    16801 caattttgtt tgaaaagacg atggatagcg atattgatgg cacgcccacc gcgcagtatc
    16861 tctgggaaga gcttggcgtt gtgccgtttt tgaaagttga taaggggctg gccgaacagg
    16921 ccgacggtgt gcagttgatg aagccgatgc cggagctgga tggcttgctg gaacgtgcaa
    16981 cagccaaagg cattttcggc accaagatgc gctctgttat tcattcagcc tccgaaagcg
    17041 gcattgcggc ggtgatcgcg cagcaatttg aagtgggtaa gcagatttta agtcatgggc
    17101 tggtgccgat tatcgagccg gaagtgaata tcaacgcgga cgataaagcc gattgcgaac
    17161 gcattatgaa ggcggaaatt ttgaagcaac tggatgcttt gagtgatgat cagaagatta
    17221 tgttgaaact gaccttgcct gaagaggcaa atttctttgc agagttgatc tcccatccaa
    17281 atgtattgca ggtggtggcg ctgtcagggg ggtatgatct ggccgaagct tgtcgccgtc
    17341 tggctgaaaa tgacgggatg attgccagct tctcgcgggc cctgtccaac gatctacggg
    17401 cgcagcagag cgatgaagag tttaatgcgg cgctgagcaa tgctattgat aagatctata
    17461 ctgcttctgt cggtaaggaa gccggggcgc aggcggctta aaatggattt aataagcgca
    17521 ttatttccta ctctgtttgc taaaacattc tttattctgg gaacgcagct ttttgtgaca
    17581 tggctcgccg cgaaagctac tttgtcaaaa ttccggcaat tgcacgactg gggaactcct
    17641 tggattacct ctactacaaa tgaaaataga catcgggacc ttcatgtaga ttggaaacat
    17701 gtggcgacgc ctttttatgc gcttttaatc gtcaattttg cagcgttcct gctcctgctt
    17761 ttctggggtg tgaaacagcc gctttctatc tctttcggtc tttttgattg aagacggccg
    17821 tttgactaat tttgaccaga gtgtgtatcg acagcttatg cgaaaaatgg atcaagatgt
    17881 gctgactttt tctgcactgg ggtcttcaca agagcaaaca gccgaggttg agcgcatgta
    17941 cgagagactg cacgccaaaa gcttcttgag ctttcacaaa cgactctttc tagtcctgcg
    18001 tccacagaac ttgcacaatc tctttagcgc cggtggcgag cataataagg cgatcaacac
    18061 cgagggctat tcctgcgctt tcgggcatgc cgtgatcgag cgcggcgagg aaatcttc
//
LOCUS       DAOSWR010000024        18073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1975_length_18073_cov_5.655178, whole genome shotgun sequence.
ACCESSION   DAOSWR010000024 DAOSWR010000000
VERSION     DAOSWR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 18073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18073
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1975_length_18073_cov_5.655178"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..2492)
                     /locus_tag="PK513_03130"
     CDS             complement(81..2492)
                     /locus_tag="PK513_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81479.1"
                     /translation="MQKYESTNIKVKMHISIRKLGVFLVLPFLVLVSFWGAESYVSSA
                     QASFFDKFWKKEASQYAVLAELSGFEPGAKVSYRITGPGKEPILNAAQINQDGTLLVP
                     APAGSQIQNKNITYDFSINEAERTVSLLLRHNALTGATSLNGDTDRPFTDVQIQAQDR
                     EIQKRSDWAGLFEETGIKLKADKDVVQPVQIAFYSRDIATDAREYASPALIKILIAPG
                     GGGPTGSGLNGWENIGADLTSADLANLSNVANNTANQISSFIGTPNGLAADLRDQGIL
                     TNIADDAVGGFNYCQFNICAASSILDLADLAETVGDYNLGLELYEIRNLANTVDSLNN
                     LPATVNQLRTLSSAATTANPNSLSSLGNLVNIVSNIGASQDITTLANLAASIPQINTL
                     ANLAASSGLSNLAADLNKLAALAALADSFSDFAVSLGFCSFTMPGSTCNTAALNAANS
                     GIVSNYVYALMMMTEQLSAVMMQQVEIIGTFFDAKHQLESQRLQQELKAQAVKDYHPS
                     DQMCRIGSYMRSLATIEEKVSYDHMALNDILIEKETNRHNVSSALGPEDDLKTRLEQY
                     KTTYCDPKDNNAGLDLLCKHAGGLGGANKQRVNKDIDFARALDYPYTINIDFADSTMT
                     DDEADIIALGQNLYWHDVFSYMKSEQAKEKSKDYMKARQMLTLNNLAHNSFTKQAAMK
                     AKAKAPTAGIEPGWAYMKTMLRELGLADSDIESMIGDRPSYYAQMDVLTKKIYQNPDF
                     YTNLYDKPTNVDRVGAAIEAIALMQMRDHYEATLRREMLMSGLIEAELIPDVETLRGQ
                     MLDLK"
     gene            complement(2498..3823)
                     /locus_tag="PK513_03135"
     CDS             complement(2498..3823)
                     /locus_tag="PK513_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81480.1"
                     /translation="MIVAIFMAVMPSVIVFSPPKAQAHCCSQYYQCVQCISQSRARVA
                     SEWSKTIQQVNQFTTDEFIAHREWIVAILWEDNILPALMMMADQFSAVAMQQTQAIGS
                     FFDAKHQLETQRVLQTVRARAHKDYHPSAGICEFGTSVRSLAASERKSELNAHVMSQR
                     SQDRQLGNANVAAASGPDADYNNRLEQYKEKFCDPKDNNAGLDLLCKHAGGLGAAKNE
                     RKNKDIDFARTVEYPWTLNIDFTDSNLTAEEEEVMALASNLYGHDVFQRISPPDKLKT
                     GSESEKGAQSVYLDSRAFLAKRSVAENSFNAITSMKSAGTKGSRDFLEAILKELGVKD
                     TGAAPNDILRMLGMDEANNEIGPSYYAQMEVLTKKIYQNPDFYTNLYDKPANVDRKRV
                     AMQAIGLMQKFDLFKSYLRNEANLSVLLELAVVDLQNEIENELSSQPQN"
     gene            complement(3896..4981)
                     /locus_tag="PK513_03140"
     CDS             complement(3896..4981)
                     /locus_tag="PK513_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81481.1"
                     /translation="MTAVAMHQTLIIGEFFDAKHQLETQRLFQELQVQAHKDYQPSTA
                     FCAFGTNARSLAHAESLGRYNAIAIGQRQLARHLGQVNMAGSREAADDKAARWKQFSE
                     IYCDPQDNNWLAGVANTGLISGGADPVCKPKGSRTTSLNRINIDIDYTRALENRRTID
                     VGRPIDVGSDDEIDTQALGNNLYGHDILFRNISRANIKEADKEKLYMSLRSIAAKRNV
                     AENSFNNIVGLKSIGNVRGYFSNTPVETYKYLAAILVELGIPENEILEYTGDFGSTPE
                     NIDFSYYNQLEILAKKIYQNPDFYANLYDKPANVKRISTALKAIELMLDRAIYESQLR
                     QEMSMSVLLSSRLRLGFKDIDKNLQGK"
     gene            5505..6197
                     /locus_tag="PK513_03145"
     CDS             5505..6197
                     /locus_tag="PK513_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system apparatus protein
                     IcmL/DotI"
                     /protein_id="HOO81482.1"
                     /translation="MAAKKNIKTDQVPEKSAKGAPEADTSGLGSVIVRNEFYRDGYRS
                     LLRLALLQGLASLGLLGAMYFVIQVHQPENRYFATTEDGRLVPMVPLNEPNLSTPALM
                     SWVAQAATEVMTFGFNDYRRRLQESSRNFTRRGWESFTGALQRSRIIEMVEANQQVVT
                     AAPQGAPIVVSEGIVAGRYQWQIQMPMLLTYQSGARTRTDKLLVTIVVVRVPRLESPN
                     GVGIEQWIAIPN"
     gene            6364..7431
                     /locus_tag="PK513_03150"
     CDS             6364..7431
                     /locus_tag="PK513_03150"
                     /inference="COORDINATES: protein motif:HMM:NF023713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HOO81483.1"
                     /translation="MSKVFPAKILYKPLLYGACFAGLCALGGFDALAQMPFSGRNDSA
                     SAPPSPTINLPTTGADQPFAFDQQDGALDFEESAEEKKEQARSEAFDAALEGLLPLRP
                     EEIRTLLERYDRTQESVETPIYPNPKPEVVVQNISLDPGAEPAVIKMAYGHVTTVAFL
                     DTTGAPWPVQDISWAGNFEVIDVQQSEDRFTHVFRISPQSEFAFGNMSIDLTGFMTPV
                     ILTLETGRDTVHYRFDAVIPDYGPLAEAPLIDSGITTTAGSEDISSFLKGVLPQDAKK
                     LDVSGVDGRTSAYRYNDVVYVRTPLTLLSPGWSSSAASADGTRVYEISDTPVLLLSDR
                     GRMVRAKLSERSDILGDILDE"
     gene            7424..8707
                     /locus_tag="PK513_03155"
     CDS             7424..8707
                     /locus_tag="PK513_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HOO81484.1"
                     /translation="MSDDLDDDLSLDETFDNFDKKGNTLGDMWRNNPAFKVGVIVVGA
                     VLLFLMITLLGGDDVPVEESYVGAAPDITSTPGTEEASPAYVQAIEELNEADIERAMT
                     EGDSSLPVPIEPPISIVSLPEQDAQEEDPLQRWRRLQEERLEREIQKQTALPEDTNVD
                     ADRLESIQQLAEQMSGQMSAVLETKAETMLSTKTLTSPDFLEKLKESQEKNAGDGELL
                     EDSEFTDEELVGELLMPAGEIVYAQLLTEANTDVPGPVLAEIMSGPLKGSRILGTFEE
                     QTELLTLNFDTVVYEDQSLSVDAVALDPATTLPGMATEVDHRYLKRILLPAAAAFVEG
                     FSGAIAQTATVTVVTDGGTVVDSETEPDNTEEVALGLEEAGSKLSEIIDEMAEETKVL
                     VRIAAGTPIGILFLEPVTTDEAEVGLAAESLEAAE"
     gene            8793..10121
                     /locus_tag="PK513_03160"
     CDS             8793..10121
                     /locus_tag="PK513_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81485.1"
                     /translation="MPNKKDQIEDEFDDLDFEDLDSEESFGDDSWDEFDDAEEDSGDA
                     IDAVADGDSESKGAPSGHAKKKSFLQKNFNLIVIALAVLGGGGFIFSQMGGQLNFSSF
                     TNQAGLDIEMQQAGTLEQIPEEIPELAGDLPPMPAPIENLENEEFALSNSVEDSIASD
                     LDFELEPSSPQAEPKQVESTSQLESDAILTPLPDLTATPDTELADLDALTQENPAKGE
                     DAKGGDFTDLTVSEDDVVSALEGRPVQENSAFKTPDAEINADISLETANLKAQIKDLN
                     QTLSSNQAALDLAQETENKLSKDLQAANMKIQDLNIELEALQKQLNILKNNEKLSSSA
                     KNAPAEVEKSAPSPKKRAQEPQEKVKTAKIVPSSDDNKTELSPSETTKILEWVLKSAG
                     PGRATLSPKDSSDMRQVEVGDVVPGLGRIQSISIEDNRWIVRGEKSSVSQ"
     gene            10298..12004
                     /locus_tag="PK513_03165"
     CDS             10298..12004
                     /locus_tag="PK513_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81486.1"
                     /translation="MKAQAANFAVAFIFSIIFSVGSGSPALAQEVDDIAKNIVSGSKD
                     FPALIAAISYGLGLLLGVLGVLKLKDHVENPTQTPLRSGIIRLLAGGALFALPMIYEA
                     AANTIGLNALSFDNDSIANDISAFLGNLSGILGVNLNAVLDSIRNSIDEIPGLISALA
                     YILGLVIGVQAILKLKDHVENPDQTGLKEPVIRFLVAGALFSIPTIYNAMFDAVGGNG
                     LGIIGSITSAFGAAGFFFSSYAQNACNPIGAVTGLIPGGGASLGQTICGIVTHAGAFP
                     AFLSACGYIIGLVLGLWGIFKIRDHVLNPQQTPVSEGVMRLLAGGAFFALPVIVEVAR
                     NTLGTGFLSAASMAPISFAGYNNGGGGSIFATILNFLGLGGGCGGGGPLGLDGLLVCA
                     MGDLMCPIHVVLNFFAFCAGMVLLMIGISRLIKSAQEGARGPGGIGTIMTFLTGGALI
                     SYNELMRAFSTTFAGSPMTATFATMDYSPCAGGGLACAEANAAHATISAVLQFVIIVG
                     LVSFVRGIFIIRGVAEGNQQASIMAGVTHLVGGAVAVNLGPILNAVQTTLGLTGFGIS
                     FT"
     gene            12070..12477
                     /locus_tag="PK513_03170"
     CDS             12070..12477
                     /locus_tag="PK513_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81487.1"
                     /translation="MFKKTQKNYNKLGAFMVAGMVAGGSSDAFAAGGGGGNDFSTIAK
                     SITTSIQDFPGLLTALAYMFGILLGVLGVMKIKDHVENPTQTPLKDGAIRLAAGGALF
                     ALPMLFEAMFETLDESGAGVDAAKLNKINFNVT"
     gene            12483..13202
                     /locus_tag="PK513_03175"
     CDS             12483..13202
                     /locus_tag="PK513_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein DotN"
                     /protein_id="HOO81488.1"
                     /translation="MEHFPITLGIARPKPGSSRKQSGAGKPGSSSAMEGKLPKDLKQK
                     IFERDEHTCQCCGFQSKKYQEVLHKNFDPSDTSEKNLITTCIFCHQCFHLDAVSAMKS
                     GVLVWLPEIDQAMLHHIARAIYIARISQGPVAEAARKSLDVIMGRREEAKKRIYTDDP
                     FILASVLSDYLGPKHYDARKEKLEGLRLFPLDRRIIKEADLEFNQFPQILAYWRSKDG
                     PFGGKTPPQWISIYQQLNIAA"
     gene            13321..16329
                     /locus_tag="PK513_03180"
     CDS             13321..16329
                     /locus_tag="PK513_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmB"
                     /protein_id="HOO81489.1"
                     /translation="MSYIKKILAPIQKSMRQSVSSFINLETADNETTLVSNDGSLISY
                     LKVEGSKQIIGEEEYNNIVEGATIKIGARFDRQGHAMQVYYVRDPGRIEKVLNGLMRP
                     LKISANSIGLEIEDLIEERVSHLQRFLSHEEAYFVLWTRSSVLTKSEIARASKDTLEN
                     AKQWVNAPDSQFPYATLEPLRTRHKSYISAMTGALEELGISCNIMEVHDALNAVRSNM
                     FPDRANDKWRACLPGDPIPPRAPTSRIDMSDILWPNLPNQLVAGDARVLGKSVCRIGD
                     TYWAGGDMSLGPMDATAFPILLNRLVEADIPFRISFLIEGGGIYATQLRTFLATIMGP
                     TNTANKQIKYSLEGLQTMARSEPVVRLRVSFATWCALKEKDEIQDRLSVLLQAIESWG
                     YAQVTEFSGDALDCIMSSAMGIHCAGTAPTGIAPMKEVMKLLPWQRPSSPFKQGGSVL
                     FRTPDGKVWPYQTGTNLTTTWFDLIFAQPGAGKSVLLNTLNLGTCLSPGLNTLPFVAV
                     IDIGPSSSGLISLIREALPLNRQHEASYYRLQMSHQYAINPFDTQLGCRSPLPEERSY
                     LAELLTLLCTPPGYDKPYDGMQQLTGLVVDEMFRWRNDNEANAEPRPYLPRLEAEVDE
                     AIHKHNIHLPTDPYWWDIVDKMFDLGEYHIASLAQRHAVPTLNDSITASRRPQIRTLL
                     EETAVGFSSESVISAFERMITSAIREYPILSSVTQFDLVDSRVCSLDLMDVAPQGDEA
                     ADRQTSIMYMLARHALVRSWWMGQEAVQYVPKKYQEYHELRLQELSEAPKRLCYDEFH
                     RTSKSHSVRNQVIRDVREGRKRGVQIVLTSQLIDDFSKDMVDLATGVWVLGTAISDKA
                     VADVVDRFGLSDTAQKIIRHKLTGPKSVGAPCLFVLGSTDGRYEQHLYNTLGPIELWA
                     LSTSSEDVAIRNRLYNKIGAGRARKILAANYPGGSARSEIRRRVLANSEEGEARAAMI
                     SAIIEQIVDELVEESKNEQKTETNKRVKLF"
     gene            16342..17013
                     /locus_tag="PK513_03185"
     CDS             16342..17013
                     /locus_tag="PK513_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81490.1"
                     /translation="MSDTPKDDEDFTLSAEIMDRAERKAFREKAKLEAKEKAKKFEPT
                     QDEIEAALANSKFIEAEKAKKKKLFKGVSAFLVLVFLFWAIDSLFTPYKAGMTFGICR
                     TYLELNVQFPQDLRISTIEDFGEYIRIWYTQLDAFGEYRMENIQCHFRADEVTGAAVE
                     KILINRREVDPEKVVLFNASIPVILQNPPDLTIPAPLPNSLENLQINTDAFRFQLNLP
                     GKLGQ"
     gene            17048..18020
                     /locus_tag="PK513_03190"
                     /pseudo
     CDS             17048..18020
                     /locus_tag="PK513_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT         4624 a         3923 c         4417 g         5109 t
ORIGIN      
        1 tgcaagcagc ggctttttgc ctataaaagt ttattatgca aggaaagtgc cagtttcatg
       61 gtgtttgaaa ccgcgcagaa ttatttcaag tcaagcattt gaccacgaag ggtttcaaca
      121 tcagggatga gttcagcctc aatcaagccg gacatgagca tctcacggcg aagcgtggct
      181 tcatagtgat cacgcatttg cataagggca atggcctcta ttgctgcgcc tacgcgatcg
      241 acattcgtgg gcttgtcata cagattggtg taaaaatccg ggttttgata aatctttttg
      301 gttaaaacat ccatttgagc ataatagctg gggcggtcgc cgatcatgct ttcaatgtct
      361 gaatctgcca gccctaattc acgcaacatc gttttcatat atgcccatcc gggttcaata
      421 ccagccgttg gcgctttggc tttcgctttc atggctgcct gttttgtaaa ggagttatgc
      481 gctaaattgt taagcgttag catttgacgc gccttcatgt agtcttttga cttttccttc
      541 gcctgctcgc tcttcatata tgaaaacaca tcgtgccaat ataaattttg tcccaaggct
      601 ataatgtcgg cttcatcgtc tgtcatcgtt gaatctgcaa aatcaatatt gatcgtgtag
      661 gggtagtcca gtgcgcgcgc aaaatcgatg tctttattta cacgctgttt attggcgcca
      721 ccaaggccac cggcatgttt acaaagcaaa tccaaaccgg cgttgttatc ctttgggtcg
      781 cagtaggtgg ttttatattg ctctaggcga gtcttaaggt catcttcggg accaagagcc
      841 gaactgacgt tatggcgatt ggtttctttt tcgatcaaga tgtcattcaa tgccatatga
      901 tcgtaactca ctttctcctc tatagttgcc aggctgcgca tataagagcc tattcggcac
      961 atttgatcgc tggggtgata atctttaacc gcttgcgctt ttaattcctg ctgaagtcgt
     1021 tgcgactcaa gctggtgctt cgcgtcgaag aatgtgccaa tgatttcaac ctgttgcatc
     1081 ataactgcag aaagctgttc ggtcatcatc atcagagcat acacgtaatt agaaacaatt
     1141 cctgagtttg ctgcattgag cgccgctgta ttacatgtgc tgcctggcat tgtgaaagag
     1201 caaaaaccaa gagaaactgc aaaatcgcta aatgagtcag caagagcggc aagagcggca
     1261 agcttgttaa gatcggcggc aagattgcta aggccagaag aggcggcaag gttggcaaga
     1321 gtattaattt gagggatcga agcagcaagg ttagcaagag tggtaatatc ctgtgaggct
     1381 ccaatattac tgacaatatt aacaagattg cccaggctgg aaaggctgtt tggattggcc
     1441 gttgttgccg cagaactaag cgttcgaagc tgatttacag tcgcagggag attgttaaga
     1501 ctgtcaaccg tattggcaag atttctaatc tcgtaaagct cgagacctag attgtagtca
     1561 ccaactgttt cggcaagatc ggcaagatct aaaatgctag aggccgcgca tatgttgaat
     1621 tgacaataat taaacccgcc aacggcatcg tcagcaatgt tggtcagtat gccttgatcg
     1681 cgcagatcag ccgctagccc atttggcgta ccgatgaaag agctaatctg gtttgctgtg
     1741 ttattcgcaa catttgaaag attggcaagg tcggcagacg tcaagtctgc gccaatattt
     1801 tcccaaccgt taaggcccga acctgtcgga cctccccccc ccggagcaat gaggattttt
     1861 attaaggctg ggctggcgta ttcacgagca tcggttgcta tatcacgaga ataaaatgca
     1921 atttgtacgg gctgtactac gtccttgtcg gccttaagct taatgccggt ttcttcaaat
     1981 agccctgccc aatcgctgcg cttttgaatt tcgcggtctt gagcctgtat ttgaacatcg
     2041 gtaaaggggc gatctgtatc gccatttagt gatgttgcac ctgtcagggc attatggcgc
     2101 agcagtagcg aaactgttcg ctctgcttca tttatagaaa aatcatatgt tatatttttg
     2161 ttttgtatct gtgaccctgc gggcgccgga accaaaagtg tgccatcttg gtttatttgc
     2221 gctgcgttta aaatcggctc ttttccgggg ccggtaattc tatagctcac ttttgcaccg
     2281 ggttcaaagc cggaaagttc ggccaaaaca gcgtactgcg aggcttcttt tttccaaaat
     2341 ttatcgaaaa acgacgcttg tgcactagaa acataagatt ctgctcccca aaaggaaacc
     2401 aagaccaaaa atggcagaac taaaaaaact ccgagtttcc ttattgatat gtgcatcttg
     2461 accttaatat ttgtgctctc atatttttgc attcacgtta gttttgcggt tgtgagctca
     2521 actcattttc aatttcattt tgcaaatcaa ctacagccag ttccaagagg acggataagt
     2581 tggcttcgtt gcgcaaataa cttttaaata aatcaaattt ttgcattaaa ccgatggcct
     2641 gcatcgcaac tcttttacga tcaacgttgg ctggcttgtc atacaagttg gtgtaaaaat
     2701 ccgggttttg ataaatcttt tttgtcaaaa cttccatttg tgcgtagtag ctggggccaa
     2761 tttcattgtt agcctcgtcc atgcccagca tacgcaaaat atcattcggt gccgcccctg
     2821 tatcttttac cccaagctcc ttgagaatag cttccagaaa atcacgtgaa ccttttgttc
     2881 cagcggactt catagatgta atcgcattaa aagagttttc cgcaacgctg cgctttgcta
     2941 aaaatgcacg ggagtccaga tagacacttt gcgcaccttt ttcactttca gagccagttt
     3001 tgagcttatc cggcggcgat attctctgga aaacgtcgtg gccgtataaa ttgctggcca
     3061 gtgccatcac ttcttcttct tctgctgtta aatttgaatc cgtaaagtct atgtttagtg
     3121 tccatggata ttcaaccgtt cgagcaaaat caatgtcctt gtttttacgt tcattttttg
     3181 ctgcgccgag accgccggca tgtttacaaa gcaaatccag tcccgcgttg ttatcctttg
     3241 gatcgcaaaa tttttccttg tattgctcca gccggttatt gtagtcggca tccgggccgg
     3301 atgcggctgc tacattcgca ttcccgagct gacgatcttg cgaacgctga ctcatcacat
     3361 gcgcatttaa ttcgcttttg cgttctgaag ctgccaagct cctaacgctg gttccaaatt
     3421 cacatatacc ggcgctgggg tggtagtctt tatgcgcccg ggcgcgcact gtttgtagaa
     3481 cacgctgggt ttcaagctgg tgttttgcat caaaaaacga accaatagct tgcgtttgtt
     3541 gcatggcaac ggcagagaac tgatcggcca tcatcatcag agccggcaaa atattatctt
     3601 cccaaaggat cgcaactatc cactccctgt gcgctataaa ctcatctgtt gtaaactgat
     3661 ttacttgttg aatcgttttg ctccattcag aagcgactcg cgctctagac tgactaatgc
     3721 actgtacaca ttgataatat tggctgcaac aatgcgcctg cgctttcggc ggagaaaata
     3781 caataacgga cggcattacc gccatgaaaa tggcaacgat cattatcagt tttttgattt
     3841 tcacgcttat gcttatcatg ttcgttattc gcccttcaca ttcctcattt gtttattatt
     3901 tgccctgtaa gtttttatca atgtctttga atccaagcct tagacgcgaa gatagtaata
     3961 cggacatgga catttcctga cgcaactgac tttcgtaaat cgcgcggtcc aacatgagct
     4021 caattgcttt taatgcggtg cttatgcgct tcacgttggc cggcttgtca tacaaattgg
     4081 cgtaaaaatc aggattctga taaatctttt tagctaaaat ttccagttgg ttatagtaac
     4141 tgaaatcaat attttcaggt gtggagccaa aatcacctgt gtattcgaga atttcatttt
     4201 caggtatgcc gagttcaacc aaaatagcgg ctaaatattt ataagtctca acaggcgtgt
     4261 ttgaaaaata gccgcgtacg ttgccgattg attttaagcc gacaatatta ttgaaagaat
     4321 tttcggcaac attgcgttta gccgcaatag agcgcaggct catatagagc ttctccttgt
     4381 ctgcctcctt gatattcgcg cggctgatat tgcggaataa aatatcgtgg ccgtaaagat
     4441 tgtttcctaa tgcctgtgta tcaatttcat cgtcagaacc aacatctatg ggtctgccaa
     4501 catcaattgt gcggcgattt tccagcgctc gtgtataatc aatgtctatg tttatgcggt
     4561 tgaggcttgt cgttctggaa cccttgggtt tacaaacagg atccgcccct cccgagataa
     4621 ggcctgtgtt ggctacgcct gccagccagt tattgtcctg cgggtcacag taaatctcgg
     4681 agaattgttt ccagcgcgct gctttatcat cagcagcttc gcgagaacca gccatattga
     4741 cttgccccag gtgccgggca agctggcgct ggccaattgc gattgcattg tagcggccaa
     4801 ggctttccgc atgggccagc gaccgcgcat ttgtaccaaa agcacaaaat gccgtacttg
     4861 gctgatagtc tttgtgggcc tggacttgta attcctgaaa taaacgctgg gtttcgagct
     4921 ggtgcttcgc atcgaaaaat tcaccgataa tcaaggtttg gtgcatggcg actgccgtca
     4981 tctgttctgt aaacagctgg agggccggta atatgtgcct cagaaagaac gtgttaacca
     5041 accaatcgcg atgtctgtta aattggttag cggtgtggct ggttatattc tgccttccac
     5101 tattgtgctt actcgtaata taggaaggaa gcgtacagga aaatgcgtgc gccttttgcg
     5161 gggctagcat agcgactaaa gttgaaatac agacgataca cagcagaccg aacgccttgc
     5221 gcctacccca tgtaaaaggc tgtaaggaaa aaccaatttt catacctcta cgttcaagcc
     5281 ccattaataa attccaaaac caccgctgac caataccatt tctataaaag cgcggcggta
     5341 gtttgctcac atctttatat tatatcacaa gcttttataa aaaggcacgt ttcaaaatgt
     5401 gaaaaaattt aataataata atcctgtcgc ttgtgtccca gtaaattttc gtccacactc
     5461 ggctttataa tttgcgtgta aaccaaaaaa aattgtgtct tcaaatggct gccaagaaaa
     5521 atataaaaac cgatcaagtt cctgaaaagt ctgctaaagg cgctcctgag gccgatactt
     5581 ccggtttggg aagtgttatc gtccgtaatg aattttaccg tgatggctac aggagtcttt
     5641 tacggcttgc tttattgcaa gggctggcct cgttagggct gctaggggca atgtatttcg
     5701 ttattcaagt gcaccagcct gaaaatcgtt actttgctac aaccgaggac ggacggctgg
     5761 ttcctatggt tcccctgaat gaaccaaatc tcagtacgcc ggcattgatg tcatgggttg
     5821 cccaagcagc gacagaggtt atgacttttg gattcaacga ttatcgccgc cgtttgcaag
     5881 aatcatcgcg aaactttacg cgccgcggct gggaaagctt tactggcgct ttacagcgtt
     5941 cacgcattat tgagatggtt gaggcgaatc agcaggtcgt taccgccgcg ccacaagggg
     6001 cgccgattgt tgtttccgaa ggaattgtcg ccgggcgcta tcaatggcag atacagatgc
     6061 cgatgttgct cacttatcaa tcaggggcac ggacgcgaac tgataaattg cttgtcacga
     6121 ttgttgttgt gcgcgttccc agacttgaaa gccctaacgg ggttggaatt gagcagtgga
     6181 tcgctatacc gaactagcga ttttgccttt gtcttgaaaa ttgccatttt gcaaattcta
     6241 ttcttcttaa aaatatgatc tgtgtgtttt tctggattta ggtcgattct ctggtgacag
     6301 tattttttta tctgtctatc ctgattctgt gtttgcatat gtcatattaa gtgtttttca
     6361 ttcatgtcta aagtatttcc ggctaaaatc ttatataaac cccttcttta cggcgcatgt
     6421 tttgctggtt tgtgtgcctt gggtggtttt gacgcgttag cgcaaatgcc gttttccgga
     6481 cgtaatgata gcgcttccgc tccgccctct cccacgatta atcttcccac tacgggagcc
     6541 gaccaacctt tcgcttttga tcagcaagat ggcgcgcttg actttgagga aagcgcggag
     6601 gaaaagaaag agcaggctcg ctctgaagct tttgatgctg ctcttgaagg gcttttgccc
     6661 ttgcggccgg aggaaattcg cacgcttttg gagcgttatg atcgcactca ggaatctgta
     6721 gagacaccta tttatccaaa tccaaagccg gaagtggtgg ttcaaaatat aagtcttgat
     6781 ccgggcgcag agcccgcagt tattaaaatg gcttatggac acgttacgac tgtagccttt
     6841 ttagatacaa ccggtgcgcc gtggcctgtt caagatatta gctgggccgg taattttgag
     6901 gttattgacg ttcagcaaag cgaagatcgt ttcacgcatg tctttcgtat atcccctcaa
     6961 tcagagtttg cgtttggcaa tatgtcgatt gatctcaccg ggtttatgac gccggtgatt
     7021 ctaacgctag agaccgggcg cgatactgtg cattatcgat ttgatgccgt tattcctgat
     7081 tatggacctt tggctgaggc gcctttaatt gactccggca ttaccacaac cgcgggcagc
     7141 gaagatatat cgtcctttct taaaggtgtc ttgccacaag atgcaaaaaa gcttgatgtt
     7201 agcggcgttg acgggcggac ttccgcttat cgttacaacg acgttgttta tgtcagaaca
     7261 ccattgacac ttctttctcc ggggtggagc agttctgcgg cgtcagccga cggaacgcgc
     7321 gtttatgaaa ttagcgatac gcctgtttta ttactttctg atcgtggtcg catggttcgc
     7381 gcgaagcttt ctgagcgctc tgatatttta ggagatattc tggatgagtg acgatctcga
     7441 tgatgattta agtctggatg agactttcga taatttcgac aagaaaggta atactcttgg
     7501 ggatatgtgg cgtaataacc cagcctttaa agttggagtt attgtcgttg gcgctgtttt
     7561 actgtttctt atgattacct tattgggtgg tgatgatgtt cccgtggaag aatcttacgt
     7621 tggcgcagca ccagatataa cctcaacgcc aggcacggaa gaggcttcgc cagcttatgt
     7681 gcaagcaatt gaagaattaa atgaagcgga catagagcgc gcgatgactg aaggcgatag
     7741 ctctttgcct gtgccaattg agcccccgat tagtattgta agccttcctg aacaagacgc
     7801 acaggaagag gaccctctgc aacgctggag acgtttgcaa gaagaacgcc ttgagcgaga
     7861 gatacagaaa caaaccgctt tgccggaaga taccaacgtg gatgctgacc gattggaatc
     7921 tattcaacag cttgcagagc aaatgtccgg ccagatgtca gctgttcttg agactaaagc
     7981 cgagacgatg ttatcgacta aaacccttac cagtcctgat tttttagaga aacttaaaga
     8041 gagccaagag aaaaacgcag gggatggtga gcttttagaa gatagcgaat ttaccgacga
     8101 agagcttgtt ggtgagcttt tgatgccagc aggcgaaatt gtctatgcgc aattattgac
     8161 ggaagccaat accgatgttc ccggccctgt acttgcagaa atcatgtccg gtcctttgaa
     8221 ggggagccga attttgggaa cctttgagga acagactgag ctcttaactt tgaattttga
     8281 cactgtagtt tatgaagatc agagcttaag tgttgacgct gttgcgcttg atccggccac
     8341 gactttgccc gggatggcga cagaagttga tcatcgctat ttaaaacgta ttcttttgcc
     8401 tgcggctgcc gcgtttgtgg aagggttttc cggtgcgatc gcacaaactg caacggttac
     8461 tgttgtgacg gatggcggaa ctgtggttga ttctgaaacg gagcctgata acacagaaga
     8521 agttgcgcta ggcctagaag aggctggatc gaaacttagt gaaattatag atgagatggc
     8581 tgaagaaaca aaagttcttg tgcggatcgc tgccggaacg cctatcggta ttctgttttt
     8641 agagcctgtt acaacagatg aggctgaagt tggtctcgca gcagaaagtc ttgaagcggc
     8701 tgagtaaaga tatttaactc tggactgttt caagtctgct gcctagaatg ctcctttgaa
     8761 ttgccgcttt attaagagag agctttaagg ttatgccaaa taaaaaggat caaattgaag
     8821 atgagtttga cgatctggat tttgaagatc tggattccga ggaaagtttt ggagacgatt
     8881 cctgggatga atttgatgat gcggaagaag atagcggcga tgcaattgac gctgtggcgg
     8941 atggagattc tgagagcaaa ggtgccccaa gtggccatgc aaaaaagaag agttttctgc
     9001 aaaagaattt caatttgatt gttattgccc ttgccgtgtt gggcggtggt ggttttattt
     9061 tttctcaaat gggcggtcaa ctgaacttca gttcttttac gaatcaagca ggccttgata
     9121 ttgaaatgca gcaggctggt acgttggaac aaatacccga agagattcct gaattagccg
     9181 gtgatttacc gccaatgcct gcaccgatag aaaatttaga gaacgaagag tttgcgcttt
     9241 caaattcggt tgaagacagt attgcgagtg atttggattt tgagctggaa ccctcttcac
     9301 ctcaggctga gccaaaacaa gttgaaagta cgtctcaact ggaaagtgac gcgattttaa
     9361 cgcctctccc agatttgacg gcaactcctg acacggaact tgctgatctg gatgctttaa
     9421 cacaggaaaa tccggcaaaa ggcgaagatg ctaaaggcgg cgattttaca gatttgaccg
     9481 tctcggaaga tgatgttgtg tctgctcttg aaggcagacc ggtgcaagaa aattctgcct
     9541 ttaaaactcc agatgctgaa ataaacgcag atatttcatt ggagaccgct aatttaaagg
     9601 cacaaatcaa ggatcttaat cagactttaa gcagcaatca ggccgctctt gatctggctc
     9661 aggaaacgga gaataagctc tcgaaggatc tgcaagctgc aaatatgaaa attcaggact
     9721 tgaatataga actcgaagcg ttacaaaaac agcttaatat cttgaaaaat aatgaaaaat
     9781 tgtcttcttc tgcaaagaat gcccctgcgg aagtagagaa atctgcgcct tcacccaaaa
     9841 aacgcgctca agagcctcaa gaaaaagtga aaacagcgaa aatcgtgccg tcttctgatg
     9901 acaacaaaac tgagttatcc ccttctgaaa caacgaaaat tttagaatgg gttttgaaat
     9961 cagcagggcc gggccgggct acactttcac cgaaagacag ctctgatatg cgtcaagttg
    10021 aagttggtga tgttgtcccc gggctggggc gcattcaatc tatctctatt gaggataatc
    10081 gatggatcgt gcgcggtgaa aaatcctccg tttctcagta atcctttgta ataattattg
    10141 aaaccttcgg taacttcttg tttacatttt gtatgctaca ctagtagcag gtagtggaat
    10201 tgcataaaat tttattcaaa tgcgtgaaat ttgtgcaaaa catggtgtag cgagatacat
    10261 ggcggttata gagataaaaa aatattgtca gggtgtaatg aaagcgcagg ccgctaattt
    10321 tgcggttgct ttcatatttt ccattatttt ttctgttggc tccggatcgc ctgctttagc
    10381 gcaggaagtc gacgatattg ctaaaaacat cgtttccgga agcaaagatt ttcctgccct
    10441 tatagctgct ataagctatg ggcttggtct tttgctgggc gttcttggtg tcttaaagct
    10501 gaaagatcat gtcgaaaacc cgacgcaaac gccgttacgt tcgggcatta ttcgcttact
    10561 ggccggtggc gcgcttttcg ctttgcctat gatctatgag gctgcggcca atacaatcgg
    10621 cctcaatgct ctttctttcg acaatgattc aatcgcaaac gatatttcag cttttcttgg
    10681 caatcttagc ggtattcttg gagttaattt aaacgccgtc cttgacagca tccgcaattc
    10741 aatcgatgaa attcccgggc tgatttccgc cctggcttat atccttggtc tggttatcgg
    10801 ggtacaagct atcctgaagt tgaaagatca tgttgaaaat ccggatcaga ccgggcttaa
    10861 agaacccgtt attcgttttc tggtcgccgg cgcactcttt tccattccaa ccatttataa
    10921 cgcaatgttc gatgccgttg gcggcaacgg actggggatt atcggtagca ttacctctgc
    10981 attcggtgca gcgggctttt tcttttccag ttatgcccag aacgcctgta atcctatagg
    11041 cgccgttacc ggcctgattc ccggcggcgg agcgagtctg gggcaaacga tttgcggcat
    11101 tgtaacgcat gccggggcgt ttccagcctt cctatcggcg tgcggctata tcataggcct
    11161 ggttttgggg ctgtggggga ttttcaaaat ccgcgatcat gttcttaatc cgcaacaaac
    11221 accggtttct gagggtgtta tgcgtttgct ggccggcggt gcgtttttcg ccctgcctgt
    11281 tattgtggaa gttgcgagaa acacgttagg gaccggtttc ctttctgctg cttctatggc
    11341 tcctatttca ttcgcaggat ataacaatgg cggcgggggt agtatttttg ctacaatcct
    11401 taactttctt ggtcttggcg gcggttgtgg cggcggcggt ccgctggggc ttgacgggtt
    11461 gcttgtttgc gccatgggcg atcttatgtg cccgatccat gttgtgttga atttctttgc
    11521 attctgcgcc ggtatggtct tgctgatgat aggaatttca cgtcttatca agagtgcaca
    11581 agaaggcgca cgcggccctg gagggattgg aacgattatg acgttcctga ccggtggtgc
    11641 gctgatttct tataatgaat tgatgcgggc attttcaaca acatttgccg gaagccctat
    11701 gacagctaca tttgcgacaa tggattatag cccttgcgct ggcggcggtc tggcgtgcgc
    11761 ggaagccaat gcggcacatg ctaccattag cgctgtttta caatttgtaa tcattgttgg
    11821 cctcgtgagc ttcgttcgtg gtatatttat catacggggc gttgcagagg gcaaccagca
    11881 ggcctcgatc atggctggcg taacccacct tgtcggtgga gcagtagccg tgaacttggg
    11941 tcccattctc aatgccgtgc aaacgacgct gggtttaacg ggatttggta tttcattcac
    12001 gtaaggcgga tgctccgctc tcgtgggtgt aatttagtga gcaaaccttt ggtagaggag
    12061 aactaaacta tgtttaagaa aacacaaaaa aactacaata agcttggcgc gtttatggtt
    12121 gccggtatgg ttgccggcgg ctcttcagac gctttcgccg ccggtggtgg tggcggtaat
    12181 gactttagta ccattgcaaa aagtatcacg acatccattc aggattttcc tggtcttttg
    12241 accgctttgg cctatatgtt cgggattttg cttggcgttc tgggcgtgat gaaaatcaag
    12301 gatcacgttg aaaacccgac gcaaacaccg cttaaagacg gcgcgatcag actggctgcc
    12361 ggtggcgctc tgtttgccct gccgatgctg ttcgaagcta tgtttgaaac gcttgatgaa
    12421 tctggggcag gcgttgatgc ggccaagctg aataaaataa actttaacgt tacttaaaat
    12481 ttatggaaca cttccccatt acattgggga tcgcccggcc aaaaccgggc agttccagga
    12541 aacagagtgg agccgggaaa cccggctcct cttctgctat ggaaggcaaa cttcccaaag
    12601 acttaaaaca gaaaattttt gagcgtgatg aacatacctg ccaatgttgc ggttttcagt
    12661 caaaaaaata tcaggaagtt ttgcataaaa atttcgatcc gagcgatact tctgaaaaga
    12721 acctcataac aacctgcatt ttttgtcatc aatgttttca tcttgatgcg gtcagcgcca
    12781 tgaaatccgg tgtattggtt tggcttccgg aaattgacca agccatgctc catcatatag
    12841 cgcgcgcgat ttatattgcc cgcatatctc aggggcctgt tgcagaagcg gcgcggaaat
    12901 cgctcgatgt tattatgggt cgccgggaag aggcaaaaaa acgtatttat actgacgatc
    12961 cctttattct cgccagtgtt ttaagcgatt atttagggcc gaagcattac gatgcccgaa
    13021 aggaaaaact cgaaggtttg cgcttgtttc ctcttgaccg gcgtatcatt aaagaagcgg
    13081 atcttgaatt taatcaattt ccacagattt tggcttactg gcgttccaaa gatgggccgt
    13141 ttggcggcaa aacgccacca cagtggattt cgatttacca acaacttaac atcgccgctt
    13201 aaagcgcttg cacttataca agcataaaac ttcttttgga tgtttaaatc cctatgagat
    13261 cggctatttc ttggttatat ttaggttggc tgataattct aacctaacta aatatctgac
    13321 atgagctata tcaagaaaat attagcgcct atccaaaaat ctatgcggca atcggtttcg
    13381 tcttttatca atttagaaac cgctgataat gaaacgacgc tggtttcaaa tgatggttct
    13441 ttgatttcct atttaaaggt tgaaggcagc aaacaaatca tcggtgaaga ggaatataat
    13501 aatattgttg aaggcgcgac aattaaaatt ggcgcgcgtt ttgatcgtca aggtcatgcg
    13561 atgcaggtct attatgtgcg cgacccagga agaatcgaaa aggttttaaa cggattgatg
    13621 cggcctctta aaatcagcgc taatagtatt gggctggaaa ttgaagacct gattgaggag
    13681 cgtgtctccc acttgcaacg ctttttaagc cacgaagaag catattttgt gctttggact
    13741 cgctccagtg tccttacaaa gagcgaaatt gctcgtgcca gcaaggatac cttggaaaat
    13801 gcgaagcagt gggtaaacgc tcctgattct caatttcctt atgcaacact tgagcccttg
    13861 cgtactcgtc ataagagtta tatctccgca atgacaggcg ctctggaaga gctcggcatt
    13921 agctgtaata taatggaagt ccacgatgcc ttaaacgccg tacgctcaaa catgtttcct
    13981 gatcgcgcca atgataaatg gcgggcctgc ttgcctggcg acccaatccc gccgcgtgca
    14041 ccgaccagcc ggattgatat gtcggatatt ctctggccga atcttcctaa tcagcttgta
    14101 gccggagatg ctcgtgtttt gggcaagtct gtgtgccgca ttggagatac gtactgggct
    14161 ggcggagata tgagtctggg tccaatggat gcaacggcct ttcctatact gcttaaccgg
    14221 cttgttgagg ccgatattcc ctttagaatc tcttttctga ttgagggagg gggcatttat
    14281 gcaacgcagc ttagaacatt tttggcaacg attatggggc cgacgaatac cgctaataaa
    14341 cagataaagt actctctgga aggcctgcaa acaatggcac gcagtgagcc tgttgtacgt
    14401 ttgcgtgttt cttttgcaac ctggtgcgca cttaaagaaa aagatgaaat ccaagaccgg
    14461 ttgtctgtcc ttctgcaggc tattgaaagc tgggggtatg cgcaggtcac tgaattttcc
    14521 ggggatgcgc ttgattgcat catgtcttcg gcaatgggaa tccattgtgc cggaaccgcg
    14581 ccgacaggta tcgcacctat gaaggaagtg atgaaactcc tgccatggca gcgtccgtcc
    14641 agcccgttca agcaaggtgg ctcggtgctc ttccgcactc cagatggaaa ggtttggccg
    14701 tatcagactg gaacaaattt aacaacaaca tggtttgatt tgatttttgc gcagccgggt
    14761 gcaggcaagt cagttttgct taacacgctt aaccttggaa cgtgcttatc tcccggattg
    14821 aatacgcttc cttttgttgc cgttattgat attggcccgt cctcttccgg gttgatttcc
    14881 ttgatccgtg aggcgctgcc acttaacaga cagcatgagg ccagctatta ccgcctgcag
    14941 atgagccacc aatatgcaat taatcccttt gatactcaac ttggttgccg ttcgccttta
    15001 ccggaagaac gcagttatct ggcagaactt ttaacacttt tatgtacgcc gccaggatac
    15061 gataaacctt atgacggtat gcagcagctc accgggctgg ttgtcgatga aatgttccgc
    15121 tggcggaatg acaacgaggc caatgctgag ccgcggcctt atttgccgcg cctggaagcg
    15181 gaagtggatg aggctattca caaacataat atccatttgc cgaccgatcc gtattggtgg
    15241 gacattgtcg ataaaatgtt cgatcttggc gaatatcata ttgccagtct ggcccagcgt
    15301 catgccgttc cgacattgaa tgattctatt accgcatcgc ggcggccaca aattagaaca
    15361 ttgcttgaag aaacagctgt tggtttcagt agtgagagtg tgattagcgc gtttgaacga
    15421 atgattacat ccgcgattcg tgaatatcct attttgtctt ctgtgacaca atttgatctt
    15481 gtcgatagtc gcgtttgctc tcttgatttg atggatgttg caccacaggg tgatgaggcc
    15541 gctgatcgcc aaacttcaat catgtatatg ctagcgcgtc atgcattggt tcgaagttgg
    15601 tggatgggcc aagaagctgt gcaatatgtg ccgaaaaagt atcaggaata tcacgagctg
    15661 agattacaag agctgtccga agcccctaaa cgactttgtt atgatgagtt tcatagaacc
    15721 agtaaatctc attctgtgcg caatcaggtc attcgcgacg tacgcgaagg gcggaaacgt
    15781 ggcgttcaga ttgttttgac ttcacagttg attgatgatt ttagtaaaga catggtcgat
    15841 ctggcaactg gtgtatgggt cttaggaaca gccatttccg ataaagctgt tgcggacgtg
    15901 gttgatcgat ttggcctgtc cgatactgca cagaaaatta ttcgtcataa acttaccggt
    15961 cctaaatctg tcggcgctcc ttgtcttttt gtattgggtt ctacggatgg acgctatgaa
    16021 caacatttat ataatacgct tggcccaatt gaactttggg cgctctcaac atcatccgaa
    16081 gatgtggcta ttcgtaatcg gctttataat aagataggcg ctggccgcgc acggaaaatt
    16141 ctggctgcta attatccggg aggaagcgcg agaagtgaaa tacgacggcg ggttttagcc
    16201 aatagtgaag aaggggaggc gcgtgcggca atgattagcg ccattattga acaaatcgtc
    16261 gatgagttgg ttgaagaaag caaaaacgag caaaaaactg aaacaaacaa acgggttaag
    16321 ctgttctaag cagggaaagt tatgagtgac acacccaaag acgacgaaga ttttacgctc
    16381 agcgcagaga taatggacag ggctgagcgc aaagccttta gggaaaaggc caaattagag
    16441 gcaaaagaga aagctaaaaa gtttgagcct acccaagatg aaattgaagc tgccttagct
    16501 aatagtaaat ttattgaggc agaaaaggcc aaaaagaaaa agctttttaa aggcgtgagc
    16561 gcttttttag ttttggtctt tttattttgg gctatcgact ccttatttac tccttataag
    16621 gcgggtatga cttttggtat ttgccgtaca tatcttgagt tgaacgtaca gtttcctcaa
    16681 gatcttcgta tcagcacaat tgaagatttc ggggagtata ttcgtatatg gtatacacag
    16741 cttgatgctt tcggagaata tcgaatggaa aatatacaat gccatttccg tgcggatgaa
    16801 gtcacaggcg cagctgtcga aaagattttg attaacagac gtgaagttga tcctgagaaa
    16861 gtggtgcttt ttaacgcaag cattcctgtc attctccaaa acccgcctga tctgacgatt
    16921 cccgcacctc ttccgaatag tctggagaac ttgcagatca atacggatgc tttccgtttt
    16981 caattgaacc tgcctggtaa gcttggacaa taattaagtc tgttaacacg tataaaaagg
    17041 acgcttcatg aaaacccgaa agcttgggcg cacggatctg gctgtcagtc tcatttgtct
    17101 gggcacgatg acatggggca tgcaaaacag tgaggaagac ggccatgcgc agatggatta
    17161 tgcgctggat cagggcgtga atttctggaa cacagcagaa atgtatgcaa ttccgcctag
    17221 cgccgaaaca tatggccgga cggaaaaaat tattggaaca tggtttgcga aaaccgggcg
    17281 aagggcagac gttgttctgg cctccaaggt tgccggtccg ggcattggct ggattcgtgg
    17341 cggtgattat aaaattgatc gcaaaaatat tctggatgcg gttgaagctt ccttgaagcg
    17401 gttacgaacc gattatattg atttgtatca actacactgg cccaatcgcg gatcgtatca
    17461 tttctcacaa cattggcgct ataatccgca gtttgatgcg acggcggttg aagaaaattt
    17521 tatagagatt ttggaaacgc tgaatgcgtt gaccaagagc ggtaaaattc gctatgtcgg
    17581 gttgtccgat gaaaccgcat ggggaacaat gaaatatctg gagctgagcc gcaaatataa
    17641 tttaccacgc atggtttcaa ttcaaaagcg gattattggc ctggtcaccg ctggccacag
    17701 gaatgctgag cggaaaatat cttgggggcg cacgccctga aggatctcgc tggaccttgc
    17761 tgcaatcaat tacgcgtgac acgccacagg ccaatgcggc tgtggcacgt tatattgaaa
    17821 ttgcgaatga ccatggatta gagccagcac agatggcgct ggctttcgtg aattcgcggc
    17881 cctttgtgac tgcgaatata atcggtgcga ccagtcttga gcagcttaaa tcgaatatcg
    17941 cttcggttga tattgagctg tctgatggtg ttctgagaga gattgaaggc gtttatcgag
    18001 atttcccaat accgtattag agaggtggcc ccaagaattc ggacagtgag ttaagctacg
    18061 atctatgacc gaa
//
LOCUS       DAOSWR010000025        17983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1987_length_17983_cov_5.033244, whole genome shotgun sequence.
ACCESSION   DAOSWR010000025 DAOSWR010000000
VERSION     DAOSWR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17983
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_17983_cov_5.033244"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK513_03195"
     CDS             <1..261
                     /locus_tag="PK513_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOO81491.1"
                     /translation="AIKIALNGKQMTVSASSAESGSATEEVEVNGETDIEIGFNAKYL
                     LDITSQIEGEGCRLTLADPASPTIIQDNSDPSALYVLMPLRV"
     gene            428..1384
                     /locus_tag="PK513_03200"
     CDS             428..1384
                     /locus_tag="PK513_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOO81492.1"
                     /translation="MKAVFLDFDTYYPRGLDVSGLEVAVDDFVFHGFSAPEDVVERIA
                     GAEIVLTNKVVLSAEIIAQAPDLKLIVAGATGYNNIDVAAARGAGVSVCNVRGYSTPA
                     VSQHVFMLILALRSQIMAYHADVKAGRWQESRDFCFLDYPIHELAGQTLGIVGYGNLG
                     RSVEALAKTFGMEVLLADHKGLREVRGGRTPFDEVIARADVLTLHCPLTPETIDLIGA
                     QELKAMKNSALLINTARGGVVNEEALASALKDGEIAGAGVDVLSQEPPSDGNSLLDPD
                     ILNLIVTPHVAWASRQACQRLFDQVVEVLEAYKSGALMNVVN"
     gene            complement(1385..1678)
                     /locus_tag="PK513_03205"
     CDS             complement(1385..1678)
                     /locus_tag="PK513_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81493.1"
                     /translation="MQLKIIIGAFILLCALIYNFSPAQAQKKNYTPATHMWVQNYQNL
                     LFVRVNSLEECEMFTAQAAAINSTEGHCYNGERLLKKIHCTKPVKRDGTPNCS"
     gene            1718..2866
                     /gene="recF"
                     /locus_tag="PK513_03210"
     CDS             1718..2866
                     /gene="recF"
                     /locus_tag="PK513_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOO81494.1"
                     /translation="MTFIRNLKLHNFRCYERAEFSALHAGLIVLHGANGAGKTNVLEA
                     VSLLSPGRGLRGAKMAEIQSQDTPKGAGTPWAVAAQVQTEYGLVQVGTGLEPSGDKRL
                     IRVNGENVRGQAALAEYVACVWLTPQMDRLFIDSASHRRRFLDRLVFGFDPGHSGRVS
                     RYENAMRQRSKLLQEGGADASWLDSLEAQMAETGTAIAAARLDFAQRLQSACLAAENA
                     HFPTAHLAVHGTLEGVLASATALEVEEMFTYALKDSRVRDALTGGAATGPHKSDLLVH
                     YIAKDMAADQCSTGEQKALLIGITLAHARLIAGERGFAPVLLLDEVAAHLDEERRAGL
                     YALLEELSGQVWLTGTDGALFSAIEGSAQFFEVQDGAVKNLTQNRSAA"
     gene            complement(2841..3929)
                     /gene="hisC"
                     /locus_tag="PK513_03215"
     CDS             complement(2841..3929)
                     /gene="hisC"
                     /locus_tag="PK513_03215"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO81495.1"
                     /translation="MNPALKNLLRPHYIDLQGYVSAGMESDKSAEKIFLNANENPYEL
                     PGLEGFNRYPEPQPKSLAAAYANAYGVKPDQIVMTRGADEAIVILTKLFCEPHKDAVL
                     TCPPTFGMYGVDAKAMPADVLEVPLRKTDGTFTLDQKAIVNATFTPGQNIKLIYICSP
                     NNPTGTSFPHDQIAEICRETEGQAAVILDETYAEFSSQGSMTSSLADHPNLIILRTLS
                     KSYAMAGMRMGTFLSADTDFIALVKAKALDAYPLPRASVEAALHVLSDDIRPLAKANV
                     AKLIEERNRILPLLQKSHYVKYTYPSDANFLLVKMNKAEEFVKFAAENNVIIRDFSTK
                     PGTENCIRISIGTPEQNDVVLKLLNDFE"
     gene            4329..6797
                     /gene="gyrB"
                     /locus_tag="PK513_03220"
     CDS             4329..6797
                     /gene="gyrB"
                     /locus_tag="PK513_03220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOO81496.1"
                     /translation="MSEAAENLVPKRGDNDDYGADSIKVLRGLDAVRKRPGMYIGDTD
                     DGTGLHHMVYEVVDNAIDESLAGYCDRIDVILNADGSVTVKDNGRGIPVGEHKEEKVS
                     AAQVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSEYLDLNIKREGKEWYMRFKM
                     GEAGEDLKPIRDLDDGERTGTQVTFLPSPKTFTQTEYDFKTLENRLRELAFLNSGVNI
                     YLSDNRPENEEERVQTHLHYEGGIQSFVEYLDRTKKSMLEAPISIVTEDEESGVTVEA
                     ALEWTDAYHETMLCFTNNIPQRDGGTHLAGFRGALTRVVGKYAEEKGLTKKDKIKLTG
                     EDMREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVIGEKLAEWLEENPAEAKV
                     IVGKIAEAAQAREAARKAREMTRRKGVMDVSNLPGKLADCQSKDPAVSEIFIVEGDSA
                     GGSAKQARDRHNQAILPLRGKILNTERARFDKMLGSEQVGTLITALGTSIGDEFNIEK
                     ARYHKIIIMTDADVDGSHIRTLLLTFFFRYMPQVIENGYLYIAQPPLFKVKRGNSAEV
                     YLKDERELDDYLIQSALNDLVIVDGNGQTRSGEDLRDLLLQSRKLRNSINALTQRAGN
                     RRVIEQAAIAGAFDEKVQGDEKAGAAAAAKVAERLNARAAKNAKTWEGFYTLDKGYTF
                     KRVQRGVTESVRISADRVRSADAVRLQAAAEWLAEVFENPVEIRQGDKVLTTVNGPAA
                     LYDRVMEAGRKGMAISRYKGLGEMNPEQLWETTLDPNVRTLLQVNVADAVKADEIFST
                     LMGDVVEPRREFIQDNALKAEVDA"
     gene            6880..7239
                     /locus_tag="PK513_03225"
     CDS             6880..7239
                     /locus_tag="PK513_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81497.1"
                     /translation="MWASGILTEETQSFMVPKVVIFMVVKAMKTGLDIFDNVSGKHQR
                     WKERKVLAIVADAILNHGCYTPRLVSDRTEIDDFKVVNVLRDFQDRGAICILAGGSLE
                     IVQFEVTDESYLRTLIS"
     gene            7301..7855
                     /locus_tag="PK513_03230"
     CDS             7301..7855
                     /locus_tag="PK513_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOO81498.1"
                     /translation="MRGLVLAVVMVLVSAPIFAGVARAEMTLSSSAIAEGTSIENAQV
                     FNGFGCTGENISPTLSWDGVPEVAKSLALTVYDPDAPTGSGWWHWVVFNLPIETRALP
                     AGASKSEAMPEGAVESMTDFGAPGYGGPCPPVGDAPHHYIFTLHALDVETLDLGESTP
                     AAQVGYFLNAHSVGKASFTGMYSR"
     gene            7870..8463
                     /locus_tag="PK513_03235"
     CDS             7870..8463
                     /locus_tag="PK513_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81499.1"
                     /translation="MLKKLSALVNVAHQRDQLIKFGLLLGVLAAYFGYLSWEYDLATG
                     GTLALLSWSFFVLCTPVADAGFLLDFPVRLITGVKMLYTEIAVWVLAFGINIWAMNFA
                     VDAYDKTALTRLFHEILVTPWPHWGIIVLCGMGTFLSVIFGDEVLDVASHKARVQHHK
                     HGFVWRVLAVVTFFGLIVAAYYHLIESLGIEKIINGG"
     gene            complement(8505..8858)
                     /locus_tag="PK513_03240"
     CDS             complement(8505..8858)
                     /locus_tag="PK513_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81500.1"
                     /translation="MSYYDWTLEQNAEQYDGLTLHWYFPRTGNTPMTPGDKKLNEDRQ
                     EAQRFLELGVVNNQGRFDTQRVQSHLTKVFGKNAVLVGIKSSKPDMFNGWRCDDIRFD
                     WVDKTEQPDPTETVS"
     gene            8949..10712
                     /gene="argS"
                     /locus_tag="PK513_03245"
     CDS             8949..10712
                     /gene="argS"
                     /locus_tag="PK513_03245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO81501.1"
                     /translation="MTSLAEKLSDIVGAAFERQGLPVELGVVRVSDRPDLCQFQCNGA
                     MAAAKFLKPNNADGVASGAKKNPREVAQAVVEALSDDETFSKIEIAGPGFINLNVTDE
                     FLAVHLAEVRADERLGVAQNGAGQTVILDYGGPNIAKPMHVGHLRSSIIGDALRRMMK
                     AAGYQAIGDVHMGDWGTPMGMILSELEIIGHDGPVSMEQLAEIYPKASGDCKADEARM
                     ELARAATKRLQDGDETYTRKWRQFIDISIEGMRGNFDALGVHFDEWKGESDAHPFIAA
                     MIEDLKAGGWAVESDGAWVVPVEREDDKKEMPPLILLKKDGAVLYGTTDLATIIDRVR
                     THNPDKIVYVVDQRQSLHFEQVFRAAEKGGLVQDGRPELSFAGFGTMNGADGKPFKTR
                     AGGVMRLEDLIAMGVEKARARLEEANLAVDMDEAEREEIAHKVALAAIKFADLQNQRQ
                     SDYVFDLDRMTSFEGKTGPYLLYQAVRIQSLLRKAGDSEQWTVDGGFKIQNEDRALAL
                     LLGEMADHFELALKNTTPHVLCDYAYRLAQAFSSFYGNCHILSEEDEVLRASRLALCA
                     LMHRQLVAVLGMLGITVPERM"
     gene            10747..11646
                     /locus_tag="PK513_03250"
     CDS             10747..11646
                     /locus_tag="PK513_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO81502.1"
                     /translation="MLGINKIKIGSALLLQVTDIDEFKGLWRGLDRHTTGLQLLGDVA
                     DYGANFKQVLGPLEEHLITVDMIRAVNASQLGEKGVSDLKTMPNQLPLSRGDKVYGTL
                     DTAEPEQVEPVLRKLCEWTNDTLARRDLHPLLVAAVFAAVFLQLAPFDTGNLRTVRFL
                     ILLILLKSGYTYAPYVSLTPIMEGRAEALYGALKASQESLEAGQPDWDVWLAFFLGML
                     QVQKDTLHTRLYAKETELRGLSALSSRIMALFKDHKRLQMKEIIKLTNGRRATIKLRL
                     QELVEGGYLVRHGAGRGTFYALV"
     gene            complement(11668..12534)
                     /locus_tag="PK513_03255"
     CDS             complement(11668..12534)
                     /locus_tag="PK513_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81503.1"
                     /translation="MRQSNNPHTSLRAQRSNPDKNGLPRQAFDLHRNDRKNNRSGEAG
                     NVFVLILLGIVLFVALSLSISRGMRSDTTNNLSKQEAALAASDILAYAQKVERAVNRL
                     RRNGVSESDIDFQTDYSGTGIYLLEDGNYNCWNNNANCTSDACKVFHPDGGNISPTRF
                     ENLAQLPSGYPAAWTKPGHITIRQISIQGIGTSAPDLALNISSLNPLVCNEINRKMGI
                     STDFTTTSTTVEPTATSRPEPFGGCGGGGIFNTTNILGDEDTRFEGATAFCAPIESVA
                     GTLHFYYIILVR"
     gene            complement(12521..15052)
                     /locus_tag="PK513_03260"
     CDS             complement(12521..15052)
                     /locus_tag="PK513_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOO81504.1"
                     /translation="MALDQRKYEHKHTAILRDVRPLWTRIADALTTPSYSTAVFIMAG
                     GAILWNDWALAFADLIILFLLLYFLWLRSRDKSLAFKMPLGAKFMDPNNGRGNKPGKP
                     EGILYLGNVENTNEEVWFTNSDARTHVLYLGTTGAGKTEGLKSMVSNALAWGSGFVYV
                     DGKADTDLWSSLSSLVRRFGRDDDLLVLNYMTGNADVQAPSNTMNPFSSGSASYLVNM
                     LVSLMPESEGDNAMWKERAVALVSAMMPILTWKRDHQDMPLSVRTIRDYLSLNNVIKL
                     QRDNAVPKELREGIDGYLDTLPGFVGDAYDDEGKVKPPGPDTPQYDTNTASQQHGYLA
                     MQFTRSLQSLGDDYGYIFDTQAADVDMVDVVLNRRILIVLIPALEKSGDEIANLGKIV
                     ASTMKGMMGSALGSTVEGDSASVIENKPTHSNTPFMTVFDEVGYYTAQGMAVMAAQAR
                     SLGFCLIFSGQDLPAMKKRVREEAQSITANCNIKIFGKLEDPTETKEFFEKTVGTAIV
                     TEVSGFQMAGGTEMGSYFDTQQAGVQMRPRASYDDLRGFKEGQAVITFGDVVVDCAVF
                     YSNPGFAKAMRVTRFLALPPPDEEIMKHAGSITKLRDLMVNKSWTAQRADVAVETPPE
                     IAALKKGFEKPRKKDVTPVERAISAIVEVHAIDNEIETTPSQAAATTAPQTAPPPTTP
                     TPEASTPPAAKPQATPPATQTTPPPAENAEQSGNPMGFFGANKSAENPASPPTRTTPP
                     PATPPATKQQQPPTAAPTKTGTEDTKSWQDLIDENAQPAETPSAAQEPQPPVTPATSV
                     LPPTAEPAQNTKSALPKEIQDIIENAGDKAREALFKNSEDRNATE"
     gene            complement(15174..15737)
                     /locus_tag="PK513_03265"
     CDS             complement(15174..15737)
                     /locus_tag="PK513_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81505.1"
                     /translation="MILNLKTKKSESDASASVVDGKLILSLPDAISPVVWQMDLTQAK
                     ASALEVLHDENTGHHVLKLKTPKGETVEVAAFKDRLQAVTGLMAASGALENAHGLIRP
                     PASENTGQITTHSHVQIKQTKKERHKKWLGAVLALVILFILFTLWSATIPKQIGSNGA
                     YAPTSASIDPQTSSGVPVSADAFLGGQ"
     gene            complement(15768..17075)
                     /locus_tag="PK513_03270"
     CDS             complement(15768..17075)
                     /locus_tag="PK513_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HOO81506.1"
                     /translation="MAGGQQAENTLHENAIGWSIMLVVLAVIVWIFWYYQSDEVRNIV
                     RWIRYTEAWILQWFIMAGETVGLIDTPYKIFIYGREISFWDIFNGNEQIVRDQLARGE
                     IRSPQRSLYFGMANYNKTALTINHLELFTAATMAPMRITMIIIAGIGALWCIFSGPKT
                     HYRSRLGLEGLIHRQAKNFPIIAPFVDFNPSTQPPRPPGSPVPAELPAFAEALGPEEW
                     LAYNNIPAPDGQIDEEAAARAFMHQLYGRWKGAMALKPYQQILLAAFCLKASRKRNEC
                     DDMLSRLALCWTHKNGLKLSQDKSLLKDARKILKNKDLSGGILSKCNQHAFVTTALLR
                     ALATARDEGGVLAPAQFVWLRGADRTLWYPLNNLGRQSFHLEAIGAMSHYKAEKMTQR
                     PIPVPKLSHAVETISEYMRSRRARPIPQLDYSKSNKRGVKKAI"
     gene            17535..17610
                     /locus_tag="PK513_03275"
     tRNA            17535..17610
                     /locus_tag="PK513_03275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17568..17570,aa:Thr,seq:tgt)
     gene            17669..17743
                     /locus_tag="PK513_03280"
     tRNA            17669..17743
                     /locus_tag="PK513_03280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17701..17703,aa:Gln,seq:ttg)
     gene            complement(17757..17930)
                     /locus_tag="PK513_03285"
     CDS             complement(17757..17930)
                     /locus_tag="PK513_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81507.1"
                     /translation="MNRILSEQDKSDEKIVKLVEEMNDISDSALWLYSARNYTLFLAL
                     VAQFAAQILSGII"
BASE COUNT         3881 a         3648 c         5614 g         4840 t
ORIGIN      
        1 gcgattaaaa ttgcgctgaa tggcaagcaa atgacggttt cggcgagcag cgctgaatcc
       61 ggcagtgcga ccgaagaggt tgaggtcaat ggtgagacgg atattgagat tggttttaac
      121 gcgaaatatc tgctggatat tacctcgcag attgaaggcg aggggtgccg gttgacgctg
      181 gccgatccgg ccagcccgac gattattcag gataattccg atccatcggc gctctatgtt
      241 ttgatgccgc tacgggttta gcaatcttcg tcttttggtc tgcaaataaa gattttctcc
      301 gctttccggt gctcgcgtat atttaatacg ctgtgcgccg gttctcgaaa atcttcattt
      361 tcgactcaaa atactttgat tgccgttttg gcttgatgtc atctgttcat tctatcagga
      421 gagtttcatg aaagcggtgt ttctggattt tgatacctat tatccgcggg gtcttgatgt
      481 ttccgggctt gaggtggcag tcgatgattt tgtttttcac ggatttagcg cgccggagga
      541 tgttgtggag cgtattgctg gcgcggagat tgttttgacg aacaaggttg tgttgtctgc
      601 tgaaattatt gcgcaggcgc cggatttgaa gctgatcgtg gccggggcga cggggtataa
      661 caatattgat gtggcggcgg cgcgcggggc gggcgtgagt gtgtgcaatg tgcgcgggta
      721 ttcgaccccg gcggtgagcc agcatgtttt tatgcttatt cttgctttgc gttcgcagat
      781 tatggcctat cacgccgatg tcaaagccgg gcgctggcag gaaagccgcg atttttgttt
      841 tctggattat cccatccatg agctggccgg tcagacgctg ggtattgttg gttacggtaa
      901 tctggggcgc agcgttgagg cgcttgccaa gacgttcggc atggaggtct tgcttgccga
      961 tcataagggc ctgcgcgaag tgcggggtgg gcgcactcca tttgatgagg tgattgcgcg
     1021 cgccgatgtg cttactttgc attgcccgct tacgccggaa acgatagatt tgattggagc
     1081 gcaggagctt aaggcgatga agaattcagc attgctgatc aatacggcgc gcggcggtgt
     1141 tgtcaatgaa gaggcgctgg cgagcgcttt gaaagatggt gagatcgccg gggcgggggt
     1201 cgatgtgctt tcgcaagagc cgccgagcga tgggaattca ttgcttgatc ctgacattct
     1261 gaatttaatt gtgacaccgc atgtcgcttg ggcgagccgt caggcctgtc agcgtttgtt
     1321 cgatcaggtg gttgaggttc tagaggccta taaatccggt gcgctgatga atgtcgtcaa
     1381 ttgattagga gcagtttggc gttccatcgc gtttgaccgg cttggtgcag tggatttttt
     1441 tgagtaatct ttccccgtta tagcagtggc cttcggttga attgatcgcg gcggcctggg
     1501 ctgtgaacat ttcgcattct tctaatgaat tcactcgtac gaacagcagg ttttgatagt
     1561 tttgaaccca catatgggtg gcaggcgtat agtttttttt ctgtgcttga gcgggtgaaa
     1621 aattatagat aagcgcacat aagaggataa aagcccctat aatgattttt aactgcatga
     1681 cgaatcccgt tttcaaatct gagttgttga tgactatatg acatttatcc ggaacctcaa
     1741 acttcacaat tttcgttgtt atgaaagggc tgaattttcg gcgttgcatg ctgggctaat
     1801 cgttttgcat ggggcgaatg gtgcgggcaa gactaatgtt ttggaggcgg tgtctttgtt
     1861 gtcgccgggg cggggtttgc gcggggcgaa aatggcggag attcaatccc aagatacgcc
     1921 gaagggtgca ggaaccccgt gggcggtggc ggcgcaggtg cagacggaat acggcctggt
     1981 gcaggttggg acggggctgg agccatccgg ggataagcgg ctgatccggg tgaatggtga
     2041 aaatgtgcgc gggcaagcgg cgctggcgga atatgtggct tgcgtttggc tgacgccgca
     2101 gatggaccgg ctgtttatcg attcggcgtc gcatcggcgc aggtttctgg accggctggt
     2161 gttcgggttt gatccggggc attcggggcg cgtgagccgc tatgaaaatg cgatgcgtca
     2221 gcgctcgaag ttattgcaag aaggcggggc ggatgcgagt tggctggatt ctctggaggc
     2281 gcagatggcg gaaacgggga cggctattgc ggcggcgagg ctggattttg cccagcgttt
     2341 acagagcgcg tgcttggcag cggagaatgc acatttcccc acagcacatt tagcggtgca
     2401 tggaacgctt gagggggtgt tggcctcagc aacagcgttg gaggttgagg agatgtttac
     2461 ctatgccctc aaggattcac gggtgcggga tgcgctgacc ggtggggcgg ctacggggcc
     2521 gcataagtcg gatttgctgg tgcactatat tgcgaaagat atggcggcgg atcagtgttc
     2581 gactggggag cagaaggctt tgctgatcgg gattacgctg gcgcatgcgc ggttgattgc
     2641 tggagagcgt ggttttgcgc cggttttatt gcttgatgag gtagccgcgc atctcgatga
     2701 agagcgccgg gctgggttgt atgcattgct cgaagaactg agcgggcagg tttggctgac
     2761 gggtacggac ggggcgctgt ttagcgccat tgagggcagc gcacagtttt ttgaagtgca
     2821 ggatggagcg gttaaaaacc ttactcaaaa tcgttcagca gcttaaggac cacatcattc
     2881 tgctccgggg tgccgatgga gatgcggatg cagttttcgg tgccgggttt ggttgaaaaa
     2941 tcacgaatga tcacgttgtt ctcggcggcg aattttacaa attcttcggc tttattcatt
     3001 ttcacaagca ggaaattggc atcggagggg tatgtatatt ttacataatg cgatttctgt
     3061 agaagaggga ggatgcggtt tctttcttcg ataagcttag cgacgttggc tttggccagc
     3121 gggcggatgt catcggacag gacatgcagg gcagcctcga cgctggcgcg gggcagtggg
     3181 taggcatcga gggctttggc tttgaccaga gcgatgaagt cggtatcggc gctgaggaat
     3241 gtgcccatcc gcatgccggc catcgcgtag cttttcgaca aggtgcgcag gatgatcagg
     3301 ttcgggtgat cagcgagcga tgaagtcatt gagccttgcg aagaaaattc ggcgtaggtt
     3361 tcatcgagga tgacggcggc ctggccctcg gtttcgcggc aaatttcggc gatttgatca
     3421 tgggggaaac tcgtgccggt cgggttgttg ggtgagcaga tatagatcag tttgatgttt
     3481 tggcccggag tgaaggtggc gttcacaatg gctttttgat cgagcgtgaa cgtgccgtcg
     3541 gttttgcgga gcggcacttc gagcacatcg gcgggcatgg ctttggcatc aacgccgtac
     3601 atgccaaagg tcggcgggca ggtgaggacg gcgtctttgt gcggttcgca gaatagtttg
     3661 gtaagaataa cgatggcttc atcggcgccg cgggtcatga cgatctggtc aggtttaacc
     3721 ccataggcgt ttgcataggc ggcggcaagg gacttgggct ggggctccgg atagcggtta
     3781 aagccttcca gacccggcaa ttcatacggg ttttcattgg cgttgaggaa gattttttca
     3841 gccgatttat cactttccat cccggcggaa acatagcctt gcaggtctat gtaatgcggg
     3901 cggagaaggt ttttgagtgc tgggttcatg atctttgctt ataagcattt gatttaaaag
     3961 gcaatagtaa atgttcttca gtttgttttt catttttgga gggggtgggg gtgtttttga
     4021 ttttgcggcg gattttgggc gcaagggtgg cgcgatgctg cagagaattt ataggaaata
     4081 attcctttgg tcaagcctaa attaagtgcg aatttttaca ggaagacgga aaggcaggga
     4141 gggtgagaag aggggctaaa aaactgcctt taaaaaatca attatttcaa tatcttaaag
     4201 tgtaaaaaaa ggcgtaaaaa aacgtgtttt ggcctgtctt tctaccagag cgtatgctat
     4261 accatatact tgcctgaaac caagagtaaa caaggctttt aaaagaattt taaggacgaa
     4321 cgaaaatcat gagtgaagca gcagaaaatc tggtccccaa gcgtggcgat aatgacgact
     4381 atggcgcgga ttcgattaag gttttgcgcg ggctggatgc cgtgcgcaaa cgtccgggga
     4441 tgtatatcgg ggacaccgat gacgggaccg gactgcatca catggtttat gaggtggttg
     4501 ataacgcgat tgatgaaagc cttgccgggt attgcgaccg gattgatgtg attttgaatg
     4561 ctgacgggtc ggtgaccgtg aaggataacg ggcgcgggat tcctgtggga gagcacaagg
     4621 aagagaaggt ttcggcagca caggtgatta tgactcagct acatgccggg ggaaaattcg
     4681 atcaaaattc ctataaagtg tccggcggtt tgcacggggt tggtgtgtcg gtggttaacg
     4741 ctttgtctga atatctcgat ttgaatatca agcgcgaggg caaagaatgg tatatgcgct
     4801 ttaaaatggg tgaggcggga gaagatttga agccgattcg ggatcttgac gatggtgagc
     4861 ggaccggcac acaggtgaca ttcctgcctt cgcctaagac gtttacgcag acggaatatg
     4921 attttaaaac gttggaaaac cgcttgcggg agctggcatt cttaaattcg ggcgtgaata
     4981 tttatttgag cgacaatcgc ccggaaaatg aagaagagcg cgtgcaaacg catttgcatt
     5041 atgaaggcgg aattcagagc tttgttgaat atctcgaccg gacgaagaaa tcgatgctgg
     5101 aagcgccgat ctcgatcgtg accgaggatg aagaaagcgg tgtgacggtt gaggcagcgc
     5161 tggaatggac ggatgcgtat cacgaaacga tgttgtgttt taccaataat attcctcagc
     5221 gcgatggcgg gactcacctg gcgggtttcc gtggcgctct gacgcgggtg gtcgggaaat
     5281 atgccgaaga aaagggtcta accaagaaag acaagatcaa gctgaccggt gaggatatgc
     5341 gcgaggggtt gacttgcgtg ctctctgtga aagtaccaga tccgaaattt agctcgcaga
     5401 cgaaggacaa gctggtgtca tcagaagtgc gtccggtcgt ggaaaatgtg attggggaga
     5461 aactggcgga atggctggag gaaaatccgg ctgaggctaa ggtgattgtt ggcaagattg
     5521 ccgaagcggc tcaagcgcgc gaggcggcgc ggaaggcacg tgaaatgaca cggcgtaagg
     5581 gtgtgatgga tgtgtccaac ctgcccggta agcttgcgga ttgccagagc aaagacccgg
     5641 cggtatccga gattttcatc gttgagggag actcggcggg tggatctgcg aaacaggcgc
     5701 gtgatcgtca taatcaggcg attttgccgc tgcgtgggaa gattttgaat accgagcggg
     5761 cgcgttttga taagatgctc ggctccgaac aggttggaac gctgatcacg gcgctgggga
     5821 cttcgatcgg ggacgagttt aatatcgaga aggcgcggta tcacaaaatt atcatcatga
     5881 ccgatgcgga cgttgacggt tcgcacattc gtacgttgct gttgacgttc tttttccgtt
     5941 acatgccgca ggtaattgag aacgggtatt tgtatatcgc tcagccgccc ttgtttaagg
     6001 tgaagcgcgg gaattcggcg gaagtgtatt tgaaggatga gcgtgagctt gatgattatc
     6061 tgattcagtc ggcgttgaat gatttggtga ttgttgatgg caacggccaa acgcgtagcg
     6121 gtgaagattt gcgggacctg ctgttgcaat cgcgtaagct gcgaaattcg atcaatgcgc
     6181 tgacacagcg ggccggtaat cggcgggtca ttgaacaggc ggcgattgcc ggggcgtttg
     6241 atgaaaaggt ccagggtgat gaaaaggctg gcgcggcggc ggcggcgaaa gtggccgagc
     6301 ggctcaatgc gcgcgcggcg aaaaatgcca agacctggga agggttttat acgctggata
     6361 agggttacac gtttaaacgc gtacagcgcg gcgtgaccga aagtgtacgt atttctgccg
     6421 atcgtgtgcg cagtgccgat gcagtgcggt tgcaggcggc cgccgagtgg cttgctgagg
     6481 tgtttgaaaa cccggttgaa attcggcaag gggataaagt gctgaccacg gtcaacgggc
     6541 cggcggcgtt gtatgaccgg gtgatggaag ccgggcgtaa aggcatggcg atttcgcgct
     6601 ataagggttt gggggaaatg aaccctgagc agctgtggga gacaacgctg gacccgaatg
     6661 tgcgtacgct gttgcaggtg aatgttgccg atgcggtgaa ggcggatgag attttctcga
     6721 ccctgatggg tgatgtcgtt gagccgagac gtgagtttat tcaggataat gcgctgaagg
     6781 ccgaggtgga cgcctgatct gtcatcccga gcgcagtcga gggatctgat tatttaacca
     6841 ctaaggccac taagctttta gtgtcatgcc cgcttcttta tgtgggcatc tgggatttta
     6901 actgaagaga cgcagagttt tatggttcca aaagtagtga tttttatggt tgtaaaagca
     6961 atgaaaaccg gccttgatat ttttgataat gtttctggaa aacatcagag gtggaaagaa
     7021 agaaaagttt tagcaatcgt tgcagacgct atcttaaatc atggttgtta cacaccgcgt
     7081 cttgtttctg atcgtacaga aattgatgac tttaaggttg tgaatgtatt gcgtgatttt
     7141 caagatcggg gtgctatttg tattcttgca ggtggcagtt tggaaattgt tcaatttgag
     7201 gttacggatg aatcttattt aagaacatta atatcatgaa tagcaaatct ctgtgactct
     7261 gtgactctgt ggtttgaaat attatcaaag gagaaagatg atgcgtggat tggtgttggc
     7321 tgtggtgatg gttttggttt cggccccgat ttttgcaggc gtggcgcggg cagagatgac
     7381 cttgagcagt tcggccattg ccgaagggac gagtattgag aatgcgcaag tgtttaacgg
     7441 ttttgggtgc acgggcgaga atatttcccc gacattgtct tgggatggcg tgccggaagt
     7501 ggccaagagc ttggcgctga ctgtgtatga cccggatgcg ccgacggggt cgggctggtg
     7561 gcattgggtg gtgtttaacc tgccgattga aacgcgcgct ttgccagccg gggcgagcaa
     7621 aagcgaggcg atgcccgaag gcgcggttga aagcatgacg gattttggcg cgcccggtta
     7681 tggcgggccg tgtccgccgg ttggcgatgc gccgcatcat tatatcttca cgttgcatgc
     7741 gctggatgtt gaaacgctgg atctgggaga aagcaccccg gcggcgcagg tggggtattt
     7801 ccttaatgcg catagcgttg gtaaggccag ctttaccggg atgtatagtc gttagtttta
     7861 atgcgctcag tgcttaaaaa actttcagcc ctcgttaatg tcgcgcatca gcgcgatcag
     7921 ttgatcaagt tcggtctgtt gttgggcgtg ctggcggcgt atttcgggta tctgtcatgg
     7981 gaatatgatc tggcgacggg cgggacgttg gcgctgctga gctggagctt ttttgtgttg
     8041 tgtacgccgg tggccgatgc cggttttttg ctggattttc cggtgcgcct gattaccggg
     8101 gtcaaaatgc tttataccga aatcgccgta tgggtactgg cgtttggcat caatatctgg
     8161 gcgatgaatt ttgccgtcga tgcgtatgac aagacggcgc tgacgcggtt gttccatgaa
     8221 attttggtaa cgccgtggcc gcactgggga attattgtat tgtgcggcat ggggacgttt
     8281 ttgtcggtga tttttggtga tgaggtgctg gatgtggctt cgcataaggc gcgggtccag
     8341 catcacaagc acggttttgt ctggcgggtg ctggcggtgg tgacgttttt tgggttgatt
     8401 gtcgcggcct attatcacct tatcgagtcg ttagggattg aaaagatcat caatggcggg
     8461 tagtttccgt ttgtcattgc tgccctttta gtccgcaaag tgctttaaga aacggtctcg
     8521 gttggatctg gttgttctgt tttatcgacc cagtcaaaac gtatgtcatc acaacgccag
     8581 ccattaaaca tatctggttt tgagctttta ataccaacca ggacagcatt ttttccaaag
     8641 acttttgtta agtgactctg cactctttgc gtgtcaaatc tgccctgatt attaacaacc
     8701 ccgagttcca gaaatctttg ggcctcttgc ctatcttcgt tgagcttttt gtcgcccggt
     8761 gtcattgggg tatttccagt gcgaggaaaa taccaatgca atgttaagcc atcatattgc
     8821 tcagcgtttt gttctaaagt ccaatcgtag taagacatgt caaatctcct tgagttggct
     8881 tatacatatg attatgtcaa caaaaacaga gctgtatttt ccaaaaggat ttgtttacaa
     8941 cacttggcat gacttcactt gcggaaaaat tatcggacat tgttggggcg gcgtttgagc
     9001 gccaaggttt gcctgtggaa ttgggcgtgg tgcgggtgag tgatcggccg gatttgtgtc
     9061 agtttcagtg taacggggcg atggcggcgg cgaaattttt aaagccgaac aacgctgatg
     9121 gcgttgcttc gggggcgaag aaaaacccgc gggaagtggc gcaggcggtc gttgaggcgc
     9181 tgtctgacga tgagactttt tcaaaaattg aaattgcggg accggggttt attaatctga
     9241 atgttacgga tgaatttttg gctgtgcatt tggctgaagt tagagcggac gagcggttgg
     9301 gtgtggcgca gaacggcgcc gggcagacgg ttattcttga ttatggcggg ccgaatatcg
     9361 ccaagccgat gcatgtcggg catttgcgca gttcgattat cggcgatgcg ttgcgccgga
     9421 tgatgaaggc ggcggggtat caggcgattg gtgatgtgca tatgggcgat tggggtacgc
     9481 cgatggggat gattttgtcc gagcttgaga ttatcggcca tgatgggccg gtgagtatgg
     9541 agcagctggc cgaaatttat ccgaaggctt caggggattg taaggccgat gaagcgcgga
     9601 tggagttggc gcgggcggcg acgaaaaggc tgcaggacgg tgatgagacg tacacccgca
     9661 aatggcggca atttatcgat atttcgattg aggggatgcg cggtaatttt gacgcgctgg
     9721 gcgtgcattt cgatgaatgg aagggtgaga gcgatgcgca cccgttcatt gcagcgatga
     9781 ttgaggattt gaaagctggc ggttgggcgg ttgagagcga tggggcgtgg gttgtgccgg
     9841 ttgagcgcga agatgataag aaagagatgc cgccgctgat tttgcttaaa aaggatgggg
     9901 ctgtgctgta tggaacgacc gatctggcga cgatcattga ccgggtgaga acgcataatc
     9961 cggacaagat tgtctatgtg gtggatcagc gtcagagttt gcattttgag caggtgttcc
    10021 gggcggcgga aaaaggcggg ctggttcaag atggtcggcc agaactcagc tttgccgggt
    10081 ttgggacaat gaacggggcg gatggcaagc cgtttaagac ccgtgccggt ggggtgatgc
    10141 ggctggagga tttgatcgcg atgggcgttg aaaaagcgcg ggcgcggttg gaggaagcta
    10201 atctggcggt ggatatggat gaggctgaac gtgaggaaat tgcgcataag gtggctttgg
    10261 cggcgattaa atttgccgat ttgcagaacc agcgtcagtc ggattatgtg tttgatctgg
    10321 accggatgac ctcgtttgaa ggcaagacgg ggccgtattt gttgtaccaa gccgtgcgga
    10381 ttcagtcttt gctgcgcaaa gcgggtgaca gtgaacagtg gacggtggac ggtggtttta
    10441 agatacaaaa tgaagatcgg gcgctggcgt tattgctggg cgaaatggct gatcattttg
    10501 agctggcact gaagaatact acgccgcatg tgctgtgtga ttatgcgtac aggctggcgc
    10561 aggcgtttag ttctttctat gggaactgtc atattttatc ggaagaggat gaagtactac
    10621 gtgcgagccg attggcgttg tgtgcgctca tgcatcggca gcttgtggcg gtgctcggca
    10681 tgttggggat tacggtgccg gagcggatgt gattgacatt tgttttaatt ggcttataat
    10741 ggctaaatgc ttggtataaa taaaataaaa atcggctctg ctctgcttct tcaagtcacg
    10801 gatattgatg agtttaaggg gctgtggcgc gggttggatc ggcatacgac ggggttgcag
    10861 ctcttgggcg atgtggccga ttatggcgcg aattttaagc aggttctggg gcctctggaa
    10921 gagcatctga ttacggttga catgatccgg gcggtcaatg cgagccagct tggagaaaaa
    10981 ggcgtgagtg atttgaaaac gatgccaaat caattgccgc tttcgcgtgg cgataaagtg
    11041 tacgggacgc tggatacggc ggagccagag caggtggagc cggttcttcg taaattgtgt
    11101 gaatggacga atgatacgct ggcgcggcgt gatttgcatc cgcttcttgt ggcggcggtg
    11161 tttgcggcgg tgtttttgca gctcgcgccg tttgatacgg gcaatttgcg gaccgtgcgg
    11221 tttttgattt tgttgatttt gctgaaatcc ggttatacgt acgcgccgta tgtgtcactt
    11281 actccgatta tggaggggcg cgcagaggca ttatatgggg cgttgaaagc cagtcaggag
    11341 agccttgagg ccggtcagcc ggattgggat gtctggctgg catttttcct gggcatgttg
    11401 caggtgcaaa aagatacgct gcacacccgg ctttatgcga aagagacgga gttgcggggt
    11461 ttatcggctt tgtcctcacg gattatggcg ctgtttaaag atcacaaacg tttgcagatg
    11521 aaagagatta tcaagcttac caacgggcgg cgtgctacaa ttaaattgcg gttgcaagag
    11581 ttggttgagg gtgggtatct ggtgcggcat ggcgcaggcc gcggtacttt ttatgcgctg
    11641 gtttagtaag caatggcggc tatgatgtta gcggacaagt atgatgtaat aaaagtgtag
    11701 tgtgcctgca acgctttcta ttggagcgca aaaggcggtt gctccttcaa agcgcgtgtc
    11761 ttcatcacct aaaatatttg ttgtattaaa tattccacca ccgccgcagc ctccaaaggg
    11821 ctctggtctt gatgttgcgg taggttcgac ggtcgtagaa gttgttgtaa aatcagtgga
    11881 aatgcccatt ttacggttaa tttcattgca aacgagaggg tttaaacttg aaatgtttaa
    11941 ggccagatcc ggtgcagatg ttccgatgcc ttgtatactg atttgacgga tggtaatatg
    12001 tccgggtttc gtccatgcag cagggtatcc ggagggaagt tgggctaggt tttcaaatct
    12061 ggtggggctg atattaccgc catcggggtg aaataccttg caagcatcgg aggtacaatt
    12121 agcgttatta ttccagcagt tatagttgcc gtcttccagg agatagattc cggtgccgga
    12181 ataatcagtt tgaaaatcaa tatcactttc agagacgcca tttcggcgca ggcggttgac
    12241 ggcgcgctcg actttttggg cgtaggcgag gatgtcgctg gcggcgaggg cggcttcctg
    12301 tttgcttaag ttgttggtag tgtcagatcg catccccctt gaaatagaaa gagacagtgc
    12361 gacgaacagg acaatgccga gcaagatcag gacgaagacg ttgccggctt cgccactgcg
    12421 gttgtttttt ctgtcattgc gatgaaggtc gaaggcctga cgcggcaagc catttttgtc
    12481 tggattgctt cgctgcgctc gcaatgacgt gtgcggattg ttactctgtc gcatttctat
    12541 cctcgctgtt cttgaatagg gcttcacggg ctttatcgcc ggcattttca atgatgtctt
    12601 gaatttcctt gggtagggca gacttggtgt tttgtgcagg ctcagcggtt ggtggtagga
    12661 cagatgtggc tggcgttact ggtggctggg gctcttgtgc agcagagggt gtttcagcag
    12721 gttgtgcatt ctcatcgatg agatcttgcc aggattttgt atcctcggtg ccggttttgg
    12781 ttggcgcggc ggttggcggt tgttgttgtt ttgtggcggg aggcgtagcc ggcggtgggg
    12841 tggttcgcgt cggcggagag gcaggatttt ccgcagattt gtttgcgccg aaaaagccca
    12901 tggggtttcc tgattgttca gcgttttctg ctggcggtgg ggtggtttgc gtcgctggcg
    12961 gcgtggcttg tggctttgcg gctgggggtg tggaggcctc aggtgttggc gttgttggtg
    13021 gaggagcggt ttgtggcgct gtcgttgccg cagcctgcga gggagtggtt tcaatttcgt
    13081 tatctatggc atgaacttcc acgattgctg aaattgcgcg ttcaactggc gttacatctt
    13141 ttttcctggg cttttcaaat ccttttttaa gggcggctat ttccggcggc gtttctacgg
    13201 cgacgtcggc gcgctgggcc gtccagcttt tattgaccat caaatcacgt agtttagtga
    13261 tcgagccggc gtgcttcatt atttcctcat ccggcggtgg cagggcgagg aaacgggtaa
    13321 cacgcatggc tttggcaaag ccggggttgg agtagaatac cgcgcaatcg acaacgacat
    13381 caccaaaggt gatgacggcc tggccttctt tgaagccgcg caaatcgtca tagctggcgc
    13441 gtgggcgcat ttgtacgccg gcttgttggg tgtcgaagta cgagcccatt tcggtgccgc
    13501 cggccatctg gaagcctgaa acttcggtga cgatggccgt gccgacggtt ttttcaaaaa
    13561 attccttggt ttctgtcggg tcttcgagct tgccaaaaat cttgatgtta cagtttgcgg
    13621 tgatggattg agcttcttcg cggacacgtt ttttcatcgc gggcaagtct tgccctgaga
    13681 agatcaggca gaagccaagc gaccgggcct gggcggccat gacggccatg ccttgcgcgg
    13741 tatagtagcc gacctcgtca aatacggtca tgaacggggt gttggagtgg gtgggtttgt
    13801 tttcaatcac ggaggcactg tcgccttcca cggtggagcc cagagccgaa cccatcatac
    13861 ctttcatggt ggaggcaacg attttaccaa ggttggcgat ttcatcgccg gatttttcaa
    13921 gggccgggat cagcacgatc aggatgcggc ggttgagcac gacatcgacc atgtcgacgt
    13981 cggcggcttg cgtgtcaaag atatagccgt aatcgtcacc caaagattgc agcgagcggg
    14041 tgaactgcat ggccaggtag ccgtgctgct gcgaggctgt gttggtatca tattgtggtg
    14101 tatcggggcc gggcggtttg actttccctt catcatcata ggcatcgccg acaaagcctg
    14161 gcagggtatc gagatagccg tctatacctt ctcgcaattc cttagggacg gcgttgtcac
    14221 gttgaagttt tatgacatta ttgagcgaaa ggtaatcgcg aatggtacgt acggaaagcg
    14281 gcatatcttg atgatcacgt ttccatgtca ggatcggcat cattgctgaa acgagcgcga
    14341 cggcgcgttc tttccacatc gcattgtcac cttcggattc cggcatcagg gagaccagca
    14401 tgttgaccaa atagctggcg gaacctgatg agaacgggtt catggtgttg gagggggcct
    14461 gtacgtctgc gttcccggtc atgtagttga ggaccagcaa atcatcatcg cggccaaatc
    14521 gacggaccag tgaggaaagc gaggaccaaa gatcggtatc ggctttaccg tcgacataga
    14581 caaagccaga cccccaagcc agcgcgttgg agaccatgga ttttaggcct tcggttttac
    14641 ctgcgccagt agttcccaga tacagtacgt gtgtacgtgc atcggagttg gtaaaccaga
    14701 cttcttcgtt agtgttctcg acattaccga gatatagaat gccttcaggt ttaccgggtt
    14761 tgttgccgcg gccgttgttg gggtccatga atttcgcacc cagcggcatt ttaaaggcca
    14821 gtgatttgtc acgggatctg agccatagaa aatacagcaa taaaaacagt atgatcagat
    14881 cggcaaaggc cagtgcccag tcgttccaaa ggatggcacc gccggccatg atgaacacgg
    14941 ctgttgaata agacggtgtt gtcagcgcat cggcaatacg cgtccacagc ggacgcacat
    15001 cccgaaggat ggcggtgtgt ttatgttcgt atttcctttg gtctaaagcc atttaattgt
    15061 cttctaaatc cttttcagtg cgttactacg ctcctaaagt agtttactat tcgtcgtcgc
    15121 ttgtgccttc tgagaagaat ttagaagaca attaagcagt gcaatagtca ttgttactgg
    15181 cctccgagga atgcgtctgc ggaaacaggt acccctgagg atgtttgcgg gtcaatactg
    15241 gcagatgtcg gggcgtatgc accgtttgaa cctatttgtt tgggaattgt tgctgaccaa
    15301 agtgtgaaga ggataaacag gattactaac gcaagcactg ctcccagcca ttttttatgg
    15361 cgttcttttt tcgtctgttt aatttgcacg tgagagtgag tggttatttg gccggtattt
    15421 tccgacgctg gcggtctgat gaggccgtgg gcgttttcca gcgcccctga cgcagccatg
    15481 agtcctgtta ctgcttgtag tcggtcttta aaggctgcaa cttctacggt ttcgcctttt
    15541 ggtgttttga gtttaaggac atggtggccg gtattttcat cgtgaagaac ttccagagct
    15601 gaggctttgg cctgcgtgag gtccatttgc cagactacgg ggcttattgc gtcgggcaaa
    15661 gagaggatga gtttgccgtc aacgacgctc gcgctcgcgt cactttctga ttttttggtt
    15721 tttaaattca gtatcatgga tttcaggctc cttggtttaa gtctttttta gatagctttt
    15781 ttgacaccgc gtttgtttga tttgctgtag tcaagctgtg ggatcgggcg ggcgcgcctg
    15841 ctcctcatat attcgctgat tgtttcaacg gcgtggctta gtttgggcac cgggattggc
    15901 cgctgagtca ttttttcggc tttgtagtgt gacatcgctc cgatggcttc gaggtggaaa
    15961 gactggcgtc ccagattgtt gagcgggtac cataatgttc gatcagcacc gcgcagccag
    16021 acgaattgcg ccggagctag gacgccgcct tcatcacgcg cggtggctag tgcgcgcaga
    16081 agagcggtgg taacaaaggc atgctggttg catttcgata gtatgccccc tgataagtct
    16141 ttgtttttaa gtattttacg ggcatctttg agcagggatt tatcttggct gagctttaaa
    16201 ccgtttttat gggtccagca cagggcaagg cggctcagca tgtcatcaca ttcgtttcgt
    16261 ttgcgcgagg ctttcaggca gaatgccgcc agaagaattt gttggtaggg tttgagcgcc
    16321 attgcgcctt tccagcgacc atagagttgg tgcataaagg ctctggcagc ggcttcttcg
    16381 tcaatctgcc catccggggc cgggatgtta ttgtatgcca gccattcttc ggggcccaga
    16441 gcttcggcaa aggcgggcag ttctgcggga accggagagc cgggcgggcg tggcggttgt
    16501 gtggaggggt taaaatctac gaaaggtgca atgattggaa agtttttagc ttggcggtga
    16561 attaatcctt caaggcccag tcggctgcgg taatgggttt tggggccgct gaagatgcac
    16621 catagagcgc cgattccggc aattataatc attgtgatgc gcatgggagc catagttgcg
    16681 gccgtgaaaa gttcgaggtg gtttatagtg agtgcggttt tattgtaatt ggccatgccg
    16741 aaatacaggc tacgctgggg gctgcggatt tctcctcttg ctaattgatc acggacgatt
    16801 tgttcattgc cgttgaaaat atcccagaaa cttatttccc gcccatagat aaagatcttg
    16861 tagggggtgt cgatcaggcc aacggtttcc ccagccatga taaaccattg caaaatccag
    16921 gcttccgtat agcggatcca gcgtacaata ttgcgtacct cgtctgactg ataataccaa
    16981 aaaatccata caataacggc tagaaccacc aacatgatgg accagccaat agcgttttcg
    17041 tgtagtgtat tttcagcttg ttgtccgcca gccaaagaaa gatcgcctct ttagaaaagt
    17101 tactcttgcc agtgtagcaa gaaaaagagg gtaaaactac tttgatgagg gtaaaatttg
    17161 cgccgttttt taaaatctgt ttgcaaaggt tgtttttttt gataagacca tggccgagcc
    17221 tttgttgaaa gggttttaag tcggtgcgct gcaggctcag ttggttttat attggtttct
    17281 ggtgcgtttg taattatggg ctctactcgt agaagcggtt aaatttaaag gggtttcgat
    17341 ctttccccgt tctgtagtag ggatataata agattgacga tatattttag gttggtgagc
    17401 cgcaatagct cagctggtag agcagttgat ttgtaattaa acggtcggca gtgtaggacg
    17461 caaaagtttc aaggggttat catcttgccc ctgcccctaa taaggcgata ataaggtgaa
    17521 cggaaatagt gtacgccgca atagctcagt tggtagagca gttgatttgt aatcatcagg
    17581 tccgcggttc gagtccgtgt tgcggcacca ttttccgcca tttttgggag gtctatgtca
    17641 ccttattagg ccttattaca tctcttgctg gggtgtagcc aagcggtaag gcagcggttt
    17701 ttggtatcgc catgcgcagg ttcgaatcct gccaccccag ccatttgcat aatattctag
    17761 attattcctg ataatatttg agctgcaaat tgggctacaa gggccaggaa aagcgtatag
    17821 tttctagcac tatacagcca taatgcactg tcactgatgt cattcatttc ttcaacaagc
    17881 tttacgattt tttcatcgct tttatcttgt tcgctaagaa ttctgttcat agaggtggcc
    17941 ccaagaattc ggacagtgag ttaagctacg atctatgacc gaa
//
LOCUS       DAOSWR010000026        17959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_1992_length_17959_cov_5.464198, whole genome shotgun sequence.
ACCESSION   DAOSWR010000026 DAOSWR010000000
VERSION     DAOSWR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1992_length_17959_cov_5.464198"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PK513_03290"
     CDS             <1..896
                     /locus_tag="PK513_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOO81508.1"
                     /translation="LHDERQVSTARDMAKMARYVIQVYPEYYRYFSRKNFTYNGKTYR
                     NHNRLMDSYSGMDGMKTGYVAASGFNLIASAVRGDRRIIGVVFGGRSSKTRNAHMKEL
                     LDQGFGKIENIRVAKAQVPLPSHKPAIAQAYAALSSPTLQQGFERLIGEGDIDPANSR
                     RIETGLMAIAAHTGEIAAAQEAMPGIRLASASPASGTARSTYQAPSVTAAARPILTNH
                     NAPWSVQIGAFTSRAATDKEIQKILKTLPHPYSNAQPSIAPLRTAEGWLFRARLSGYT
                     KDEALAACNYIRDCLPVAPQN"
     gene            905..1684
                     /locus_tag="PK513_03295"
     CDS             905..1684
                     /locus_tag="PK513_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81509.1"
                     /translation="MIVKNPFLLKQRYSETGNVLFIILLAIVLIGALTAAIQSSNRSE
                     GSNIDAETLVIRASEVQRYASELERAVTFIMQNGASESDLRFAHPDAHSDYGDLSADA
                     DPSDQVFHVSGGGASYRAAPGGINDGSAWEFYAGTHIPGMGTASRAELIAVLPNVTQA
                     FCNKINEINDQPLSLPNPPGPPEDTGASAASGSDPGACLNIGALGRFNDTRQFYTTIN
                     TVDETTFTQDPNTGAAVPAPQACVKCAIGPTYHFYHVLLAR"
     gene            complement(1688..2257)
                     /locus_tag="PK513_03300"
     CDS             complement(1688..2257)
                     /locus_tag="PK513_03300"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOO81510.1"
                     /translation="MTKQPLLSKKDCLCALAVLMLASGCSPEALNTAAYGIFPQEQTN
                     FKEQNYAAADYLIQQARTFIGRGDLLIAKPLSDTNQPSMSSTISKMIPEQIGIRLSQL
                     GYRMDLSGVATGPDINYLKPSITREEKPDFVLSGTYLRRRIEMDVSLRIVDIKAGRVV
                     ASYDYILPLNRETAELSKPQPKIVRMDNE"
     gene            complement(2250..2933)
                     /locus_tag="PK513_03305"
     CDS             complement(2250..2933)
                     /locus_tag="PK513_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81511.1"
                     /translation="MRTVVVSSMLVLALTGCKPVWDPTYMPSGYAHHQKAYKAPPGPE
                     ADTIGYEYSVAQNAAVQRQWRMVANDLILRAQAHDMLDAPVFLTTDIKPGPFASAYDF
                     ALREELRVAGVILAANQEEGAELFYSAYDPDEEGPAESLTGYNDEAAHPNKDGDFLPP
                     SKTLKFILAKTQDGIISKKVSSMQEAPLYGFKPAGYAPLHERPRELAPKQCSHHEAQA
                     TSTDPSYND"
     gene            complement(3064..3261)
                     /locus_tag="PK513_03310"
     CDS             complement(3064..3261)
                     /locus_tag="PK513_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO81512.1"
                     /translation="MMDNTPFHPKAKGKAILEEKGHKLLCLPKYSPDLNPIEQSFGAI
                     KSNWKHADKNTTLDKLVTFNC"
     gene            complement(3310..3537)
                     /locus_tag="PK513_03315"
     CDS             complement(3310..3537)
                     /locus_tag="PK513_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HOO81513.1"
                     /translation="MHYPITSRRKVLAHIEEGMSKREAARLFKISPQTLYNWLSRKDL
                     HLALAKTRHRKSKQWLAPILFEGSCKGTTVL"
     gene            3667..4584
                     /locus_tag="PK513_03320"
     CDS             3667..4584
                     /locus_tag="PK513_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOO81514.1"
                     /translation="MTRYPADIMDFMDMFPDEAACLEYLSLIRWPDGYICQRCGGRDA
                     WKMSGGLYRCRDCRYAGSVTAGTLFEGTHKPLRLWFLAIWYVVNQKNGVSALGLHKAL
                     GLGSYHTAWEWLHKLRKAMVRPGRDRLHGIVEMDETLIGGIHEGRLGRSDPRKAQVLV
                     AAEEVDGCIGRIRLACIPDASGQTLLANTLEMVEPGAAIRTYGWRDYNVLPDHGYIHE
                     PCAHKNANEQDATPAAHLVASLLKRWLLGTHQSAVSHEHLPYYLDEFTFRFNRRTSCS
                     RGKLFYRLIEQALQVGPVPAKALNTTSGR"
     gene            complement(4868..5218)
                     /locus_tag="PK513_03325"
     CDS             complement(4868..5218)
                     /locus_tag="PK513_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81515.1"
                     /translation="MRWRYQDGRFYGLQVRVFVDLARAAQVRDGIFMRSVGQNTTGLP
                     VGIYKSDEQVQTAVNKGMTRDQARKVHTSFTIRRSLNLAALGAFVGAVAGPDVWSDFD
                     TPDEPDNPESRLEA"
     gene            5323..5847
                     /gene="def"
                     /locus_tag="PK513_03330"
     CDS             5323..5847
                     /gene="def"
                     /locus_tag="PK513_03330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOO81516.1"
                     /translation="MTKMYDIIIIPDPVLKQTAQAVNCVNLDIQKQMDAMLATMYAAP
                     GIGLAANQVNLLNRVLVMDLARTDEGEEKTPICMANPEIIWESEEMSTMEEGCLSIPR
                     QYAEVQRPAHVRVKYLDYNGKEAELEASGLLSHCVQHEIDHLNGVLFIDYLSTLKRNM
                     IVRKVEKLKKQTIL"
     gene            5992..6966
                     /gene="fmt"
                     /locus_tag="PK513_03335"
     CDS             5992..6966
                     /gene="fmt"
                     /locus_tag="PK513_03335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOO81517.1"
                     /translation="MINETKPLRVGFMGTPDFAASALKTIDASAHDVVCVYTQPPRPK
                     GRGHRLQPSPVHQYADERGIPVFHPKSLKSADVQAEFTAHNLDVAIVAAYGLILPKAI
                     LNAPKYGCINIHASLLPRWRGASPIQRAIWAGDKQSGVTLIQMNEGLDTGPEIMKTAL
                     DLDLRETTTSLHDRLAALGADMVIETLDKLAIKGHLTAKEQENSGVEYAHLLKKDDGR
                     VDWPQSATQIDRQIRALNPWPGVWTKTADGQRLKILTAQPADASPLDTTQAEKNRPVG
                     MLIDKTGHVQCGQGTILCLQRVQPAGKQAMDLPAALNGGYLKIGEVLA"
     gene            6963..7823
                     /locus_tag="PK513_03340"
     CDS             6963..7823
                     /locus_tag="PK513_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO81518.1"
                     /translation="MSTTSYGLIALVMFTIGNILVVLTNAIPGLQMAAMSLFLGALGM
                     TLYQAVKGQPIRTYWRQPPRHYAFLVSGVGVYTALIYVAFRHAPPFEVNAINYLWPIL
                     LVAFATLFHTGRLNAPKIVGIVMGFIGTFLIFVPAHANGFMGGLQAGHFLALIAAILW
                     AAYSASAKNKTYPAGLMAPAMFISAFLCAGLHLMFEKTVMPVGIEWVYVLLLGFFRLS
                     YLFWDESMRGGNVVFLASLAYFVPLASTLLFIAFGYTAANIWIALGASFIVSGCIIVN
                     LRDLMRLIKR"
     gene            7820..8596
                     /gene="truA"
                     /locus_tag="PK513_03345"
     CDS             7820..8596
                     /gene="truA"
                     /locus_tag="PK513_03345"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO81519.1"
                     /translation="MTQRYRLTIEYNGADYAGWQRQEKAGPQTIQGEIEKAIKAFSGQ
                     DVTVQSSGRTDAGVHAYGQIAHVDLEDLTRPMQPFEIAKAINAHLYPQPICILSAEQV
                     SDDFHARFSAKNKRYIYRILNRQAFPAIERGFVWHIRRPLDVPAMQEAAQVLLGHHDF
                     TTFRDSECQAKTAMRTLDRLEVSAREYDTGGGIEIRIAAEAKSFLHHMVRNLAGTLSL
                     VGEGKWSAADVAAALEAKDRCAGGPTAPASGLFLERVDYE"
     gene            8589..8918
                     /locus_tag="PK513_03350"
     CDS             8589..8918
                     /locus_tag="PK513_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81520.1"
                     /translation="MSKLVKSKRVVTFVPADHTNEFAKSMAAYIPHLFGCYDSVCWWG
                     TPKTEKGMEQYRPLDREIEQTPSVRMEFSIPDDPAALENFTAALKKHHPWQEPVILIF
                     DSQIIDG"
     gene            8991..9674
                     /locus_tag="PK513_03355"
     CDS             8991..9674
                     /locus_tag="PK513_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO81521.1"
                     /translation="MRLIFCPLMIMFAISFAAPATFAQDLKTTRAWEQLEKIEKSRPL
                     RNSRYISSKAVISDRVLDRTNRVIGEVRDVVLDRNGAIFSLNIDFNRLRLGTGPILVD
                     YNDMGLRPASNGYKTSYTDDEIRVRLPQILAGLESASGQETDTYSIKNLVGRTFYSAD
                     GRKLGTIKDVLFDRNGYRAELLYVMFSHKTARGKSLAIPFREAYYEAGKLTLKNDYAK
                     TILAFAQEK"
     gene            complement(9662..10792)
                     /gene="dapE"
                     /locus_tag="PK513_03360"
     CDS             complement(9662..10792)
                     /gene="dapE"
                     /locus_tag="PK513_03360"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOO81522.1"
                     /translation="MDVVKLTQKLIACPSVTPEDAGAQVLLANFLEELGFECHHLPFA
                     EEGYEVPNLFARIGSEGPHLCFGGHTDVVPPGPEGQWTFGPFNPEVKDGVLYGRGASD
                     MKGSVAAFAVAAREYLKNNDLKGSISMLITGDEEDLAVDGTVKVLEWMEKNGHVPDVC
                     LVGEPTNPEHLGQEIKVGRRGSLNGHLTVKGKQGHVAYQHLANNPMPRLVTLLNILAR
                     HTFDEGSEFFPPTNLEITTIDVGNKATNVIPEHGWATFNIRFNDHWSSASLKTKITEL
                     LDSTGFDYKIEFEGNAESFITQPGAWSDILIGAVEDVTGFKPECTTGGGTSDARFIVN
                     YCPVIECGGINESIHQIDENAKVSDLKDLVKIYGRVLERYFS"
     gene            complement(10834..11691)
                     /gene="dapD"
                     /locus_tag="PK513_03365"
     CDS             complement(10834..11691)
                     /gene="dapD"
                     /locus_tag="PK513_03365"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOO81523.1"
                     /translation="MGQAAAQHTDLETAIESAWDARDTLSPDTSGEMREAMKQALALL
                     DTGALRVAEKTSEGWQVNQWLKKAVLLSFRLNPMQKISGGPDGSGWYDKVASKFDGWD
                     KAEFEKAGFRAVPNCVVRYSAHIAKGAVLMPSFVNVGVFVDEGTMVDTWVTVGSCAQI
                     GKNVHLSGGVGIGGVLEPLQAAPTIIEDNCFIGARSEVVEGVIVEEGSVLSMGVFISA
                     STKIIDRETGEVFRGRVPAYSVVVPGTMPGKPLPDGTPGPNLACAVIVKRVDAQTRSK
                     TSINDLLRD"
     gene            complement(11796..12575)
                     /locus_tag="PK513_03370"
     CDS             complement(11796..12575)
                     /locus_tag="PK513_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81524.1"
                     /translation="MGRFHTFSITNFRPDDKALQVNLGIIFRGVYVSGMSKTGVSGDI
                     RLNGELLQCFFSECERHFRFLEQRYGYMFFCGLLEYRNNYKMIKPLNQQEFNGPFMAV
                     TRYEKGEQAFEITYSSIQFLIEAHAFYNPIDRFELSEIMTAARKSDTALRGDWGVSDV
                     SLIKKTIREMAQSFEKHSRILLEPGDKLLNRAMTIRHKQLEQSIREKHKISIDATCQH
                     AAKAYREKNYRLVVQLLEPVKRYLKRGEIKKLERAKKLLFT"
     gene            12699..13520
                     /locus_tag="PK513_03375"
     CDS             12699..13520
                     /locus_tag="PK513_03375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81525.1"
                     /translation="MQISKAALVLIDGHKAGYLQTVETRIAKGFLHFIVLDRGPLWFD
                     GFGSAEHFDAFCTALRREFPRRPGRFMRFIPEIEDSKEIQNIMKAHDFRKKAPGYQTV
                     WLDLNNDEETLRTNLKSNWRGHLRQAEKNGLSVEWDVEGCYLDWILRGYARDKSTKGY
                     PGPSVQFMKRLAEICIKEKELLIGRALLDGKPIAGVLILFHGQSATYQIGFTSKLGRE
                     KSAHNFLLWQSLLYLKENGFSAFDLGGVNDDTAQGVKKFKTGLGGRCITLAGLYG"
     gene            13480..14328
                     /locus_tag="PK513_03380"
     CDS             13480..14328
                     /locus_tag="PK513_03380"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOO81526.1"
                     /translation="MAAAALLWLVSTADPASAGETLRQQLVYEVYAGGIHAVQAHMDI
                     DLSKKNKYSLVLDAKTRGFLGSLIPWEGSFESYGWILKDGKYQPERHQSTATWRNEAD
                     IKEYLYNKNGSFKSLRITDEHSAAELREVDDEVTNGTVDILSATLQVLDAYNEMETCA
                     GESEIFDGKRRFKQIFRHEEAVTLTESKYNIYGGPAAKCTVEVVPVNGEWHKKPRGWM
                     SIQEQGRAKGTMPTVWIAQVAENGPAVPVKIRVKTAYGTLFMHLAEYKSADTVLIAEK
                     RMPEDE"
     gene            14378..15745
                     /gene="glmU"
                     /locus_tag="PK513_03385"
     CDS             14378..15745
                     /gene="glmU"
                     /locus_tag="PK513_03385"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOO81527.1"
                     /translation="MSTKPLAIVILAAGFGTRMKSSQAKVMHTLAGKPMINWLLESCE
                     SLKPEKVVVVIGPEMPDLEKAVKPHICVVQKVRDGTGGAVRTAMPALEGFDGDVLILL
                     GDTPLITPATLSALRVAKNDHALAILGCIMDNPHGYGRLILGQEGMLENIVEEKDADP
                     EQKAIRLVNTGAFCVDGAQLSGWLDQLDSNNAQNEFYITQLPAIIREQGGVTAAAVIE
                     NPAEVKGCNTRTDLAALEKTLQTRLREDLMNEGVYMQAPDTVYLWHDTKIAPDCIIEP
                     NVYFGPGVTLESGVHIKAFSYLEGAHVKAGASVGPFARLRPDTKIGKNARIGNFVEIK
                     KSTIGPGSKIGHLAYVGDTDMGEEVNFSAGAITVNYDGFQKHRTVIGKNVMVGSNVNL
                     VAPVRIDDGAFVAAGSTITEDVPANALSIARDPAEIRQGWAAKYRKIKEAAQKKAKKQ
                     RKKAS"
     gene            15850..16110
                     /locus_tag="PK513_03390"
     CDS             15850..16110
                     /locus_tag="PK513_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81528.1"
                     /translation="MSVIPFSVHATCEGYNNAMNALEAAIAESYRPKMQPPLKYRDPD
                     NAPPVASINAVIDDLEIATQKLGAPKAPVPAQSYPRRPKPHL"
     gene            16237..>17959
                     /gene="glmS"
                     /locus_tag="PK513_03395"
     CDS             16237..>17959
                     /gene="glmS"
                     /locus_tag="PK513_03395"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOO81529.1"
                     /translation="MCGIIGILSEDSAAPLLIEGLRRLEYRGYDSAGIATLHDGVLDR
                     RRSEGKLKNLEIKLKSRPLKGTLGIGHTRWATHGGPTEDNAHPHATDKVAVVHNGIIE
                     NYAQLKEELANHQIRFETDTDTEVIVHLVDHYLTQGKSPQEAASTAFDRLDGAYAIVM
                     IFAGQDNLMIGCRQGTPLAVGYGDGEMFVASDSYALAPLTKKICFLEDGDRVTVTREG
                     AQIYTNDDTPVTREIRITAQSGATTGKGNFRHFMLKEIYEQPSVIGDTLNSFVNPATG
                     EISIPPEVLAALADAPRITMVACGTAYYACMVAKYWFEQTVGIPCDVDIASEFRYREP
                     PLASDTLCIVVSQSGETLDTLEGMRYAKSEGCKVLSIVNTIESTIERESDFVMHTLAG
                     PEIGVASTKAFTTQLTTLACIALSLAVKKGALKHEKAAVLADELRHIPQQITQLLREE
                     ENTEIIQSIARDIFENTDMLYLGRNLLYPLAMEGALKLKEVSYIHAEGYAAGELKHGP
                     IALIDDHMPIVVLAPAGDALFEKTASNLQENVARGAKVLLISDESGHAKLKELIHWGI
                     KLQPLDPF"
BASE COUNT         4561 a         4630 c         4302 g         4466 t
ORIGIN      
        1 gtctacatga tgagcgccag gtcagtacgg cgcgtgatat ggcaaaaatg gcacgctacg
       61 tcattcaggt ttatccggag tattaccgct atttttcccg taaaaacttc acctataacg
      121 gtaagactta tcgcaatcat aaccgcctga tggattctta cagcggcatg gatggcatga
      181 aaacaggtta tgttgcagct tccggcttta acttaattgc ttcggcagta cgtggcgatc
      241 ggcgaattat cggcgtggtg tttggcgggc ggagttcaaa aacccgtaat gcgcatatga
      301 aagagctgct tgatcagggc tttggcaaaa tcgaaaatat ccgcgtggca aaggcgcagg
      361 ttcccctgcc atctcacaaa ccggctatcg cacaagctta cgctgccttg agcagtccaa
      421 ctttacagca aggttttgag agattaatag gcgaaggtga tattgaccct gccaattccc
      481 ggcgcattga aaccggcctg atggcgattg cggcccacac cggagagatt gctgcggcgc
      541 aagaagctat gccgggcatt cgtttggcct ctgcatcccc ggcttccgga acggcgcgca
      601 gcacttatca agcgccaagc gttacagcgg cggcgcggcc gatactgacc aatcacaacg
      661 cgccatggtc tgtgcaaatt ggagccttta ccagccgtgc tgccaccgat aaagagattc
      721 aaaaaatcct gaagacgctg ccgcatcctt attcaaatgc ccagccgtcc atcgcacctt
      781 tacgtacagc cgaaggatgg ttgtttcgcg cacgtttgag cggctacaca aaagatgaag
      841 ctttggctgc gtgtaattat atccgcgatt gtctgcctgt cgctccacaa aactagcgtt
      901 gatcatgatc gttaaaaacc cttttcttct taaacagcgt tacagtgaaa ccggaaacgt
      961 gctgttcatt attctgctgg caattgtttt gatcggcgcg cttacagcgg ccattcaatc
     1021 aagcaaccgt tcggaaggct ctaatattga tgccgaaacg cttgtgattc gtgccagtga
     1081 ggtgcagcgt tacgcctcag agcttgagcg cgcagtaacc ttcatcatgc aaaacggcgc
     1141 aagtgagtcc gatttgcgct ttgcgcatcc cgatgcccac tcggattatg gagacctcag
     1201 cgctgacgcc gatccgtcgg atcaggtctt tcatgtctct gggggtggag ccagttatcg
     1261 tgcagcgccc ggcggtatta acgatggcag cgcatgggaa ttttacgccg gcacccatat
     1321 tccgggtatg ggtacggcaa gccgcgcgga actgattgcc gtgcttccga acgttacaca
     1381 ggctttttgt aacaagatta atgaaatcaa cgatcaacca ctttctttac ctaatccgcc
     1441 gggaccaccg gaggatacgg gagcaagtgc ggcgtcgggc agcgatccgg gggcgtgttt
     1501 gaatattggt gcgctgggga gatttaatga tacaaggcag ttctatacaa ccattaacac
     1561 cgtggatgaa acgaccttta cacaagatcc caatacaggc gctgccgtgc cagcgccgca
     1621 agcctgcgta aaatgcgcaa tcgggcctac ttatcacttt taccatgtgc tgttagcacg
     1681 ctaatcgcta ttcgttatcc atacggacga ttttaggctg aggctttgaa agctcagcgg
     1741 tttcacgatt gagcggcagg atataatcat aggaggcaac cacccttccg gctttaatat
     1801 caacgatgcg caaacttaca tccatttcaa tgcggcggcg taaatatgtt ccggatagaa
     1861 caaagtctgg tttttcttcg cgcgtgattg agggtttgag atagtttatg tccggccctg
     1921 tagccactcc agacaaatcc atacgataac cgagttgaga caggcgaatg ccgatttgct
     1981 caggaatcat tttactgatg gttgagctca tactcggttg gttcgtatcg cttaagggtt
     2041 ttgcaatcag taaatcgccc cggccaataa aagtgcgggc ctgttgtatc agataatccg
     2101 cagctgcata attctgctct ttaaagtttg tctgctcttg cgggaaaata ccataagccg
     2161 ctgtgttcaa cgcttctggt gagcatccag acgccagcat aagcaccgct aacgcacata
     2221 agcagtcttt ttttgataaa aggggttgct tagtcattgt acgatggatc tgtcgatgtt
     2281 gcttgtgctt catggtgact acattgcttc ggtgcgagtt cacgagggcg ctcatgcaat
     2341 ggggcgtaac ctgcaggttt aaagccataa agtggggcct cttgcatgct ggaaactttt
     2401 ttgctgatga tgccgtcttg cgtttttgcc aaaataaact tcaacgtttt tgaaggcgga
     2461 aggaaatcac catctttatt cgggtgagca gcctcatcat tatagccggt tagtgattcg
     2521 gccggacctt cctcatcagg atcataagca gagtagaata actctgcgcc ttcttcctgg
     2581 ttggcggcaa ggattacgcc agccacccgc agttcctcac gtagcgcaaa atcataggcc
     2641 gaggcaaacg ggccaggttt tatatctgtg gtcaagaaga cgggcgcatc caacatgtcg
     2701 tgtgcctgtg cgcggagaat taagtcattg gcaaccatgc gccattgtct ttgtacggcc
     2761 gcattttgtg cgacagagta ttcatagcca atagtatctg cttccggacc tggtggagcc
     2821 ttataggctt tctggtgatg ggcataaccg gagggcatgt aggtcgggtc ccaaaccggc
     2881 ttacagccgg taagagccaa tacaagcata gaagaaacaa caacagtgcg cataagtaaa
     2941 aatcctgatt caaaaataga tcataaagtg agtgtgaagc ttattcactg ccgattagtc
     3001 tagcaattct gtgaaggcgg gtacagagct tttgccgaag tctataaatt atagtaattt
     3061 aaattaacaa ttgaacgtca caagtttatc gagcgttgtg tttttgtcgg catgtttcca
     3121 gttggatttt atagccccaa aggattgttc gatagggttg aggtccgggg aatatttggg
     3181 caggcacaag agtttgtgcc ctttttcttc caggatggct ttgccctttg ctttcggatg
     3241 aaagggcgtg ttatccatca cgatgacgct tggttttgtc agggttccca gtaatggctc
     3301 cttgattcac tacaggacgg ttgtcccttt gcatgagcct tcgaacagga tcggggcgag
     3361 ccactgtttg cttttgcggt gccgggtctt cgcaagagcc agatgcaggt ccttacgaga
     3421 gagccaattg tataatgtct gaggggaaat cttgaaaaga cgggcagcct cgcgcttgct
     3481 catcccttct tctatgtgcg ctaaaacctt gcgacgagat gtgatcggat aatgcatccc
     3541 aaaacataac tcgtccaatc aatattttaa actactatat atcagtattt tagctacttc
     3601 agccagtaaa attacttatg cctatgcagt ttactaacct gaccacaaga tatagtattc
     3661 gtatggatga cacgttatcc tgcggacatt atggatttta tggatatgtt tccggacgaa
     3721 gcggcgtgtc tggagtattt gagccttatc cgctggcctg acggttatat ctgtcagcgg
     3781 tgcgggggtc gggatgcgtg gaagatgagc ggcggtttat accgctgccg ggattgccgt
     3841 tatgcggggt ctgtcacagc aggcacgctg tttgagggga cgcacaaacc tcttcgttta
     3901 tggtttttgg cgatctggta cgtggtcaat caaaagaacg gcgtgagcgc gttgggtttg
     3961 cataaagctt tggggcttgg gagctatcat acggcctggg aatggcttca caaactgcgc
     4021 aaggccatgg ttcgccccgg tcgcgatcgg cttcatggca tcgtggagat ggacgaaacg
     4081 ctcatcggcg gcattcacga aggccgcctg ggccgcagcg atccacgcaa agcacaggtt
     4141 ctggttgcgg cggaagaggt agacggatgc atcgggcgta tccggctggc ctgtatccca
     4201 gatgcttccg ggcaaacgct cttagcaaac acgcttgaaa tggtcgaacc cggcgcagcg
     4261 atccgcacgt acggctggcg cgactacaac gtcctgccgg accatggcta catccatgag
     4321 ccttgtgccc ataaaaacgc caacgagcaa gacgcaaccc cggcggccca tctcgtcgcc
     4381 tcgcttctga aacgatggct cctcggcacg catcaaagcg ccgtatcgca cgagcacttg
     4441 ccttattatc ttgatgagtt taccttccgt ttcaaccgca gaacatcttg ctcgcgcggc
     4501 aaactctttt atagattgat tgaacaggca ttgcaggtcg ggccggtccc ggcaaaagcc
     4561 ctaaacacta catctggtag gtaggacact catctgcata ggcatgaatt actataatct
     4621 gctgattgag ttatttgaat ctctttcggg tttaattcct cgtcgcttgc ggcgtaatcg
     4681 tcatcgcgag cgaagcgaag cgatccagtg ccacaaggat aaattctgga ttgcttcacc
     4741 gtctttggcg gtttgcaatg acgtgatacc tcgccccttg gggcggaggt gttcattgcg
     4801 tttgaactca aaaaatcccg cgccctcttg ccccgatctc cgatgataac cgtgagaaaa
     4861 aacgcgccta ggcctcaagg cgcgattcag ggttatcagg ctcatccgga gtatcaaaat
     4921 cactccagac gtcaggacca gctacggctc ccacaaaggc tcctaatgcc gcaaggttaa
     4981 ggcttcgacg tattgtaaag cttgtatgca cttttcgggc ctgatcgcgc gtcatacctt
     5041 tattgacggc agtttgaacc tgttcatcgc ttttatagat acccacgggt aaacccgtgg
     5101 tgttctgtcc tacggaccgc ataaatattc catcccgcac ttgcgccgcc cgcgccaagt
     5161 ccacaaacac ccgcacctgc aatccgtaaa aacgcccgtc ttgataacgc catcgcaata
     5221 ctcctacatt tgaaaaacat tatgcctaag ttaaataaga atgcttgtta accgcaacgt
     5281 tgtaagcttg accgcacgag caaaaccgat tacctcagtt ctatgaccaa gatgtacgac
     5341 atcatcatca tccccgaccc ggtgctcaaa caaactgctc aggccgtcaa ctgtgtcaat
     5401 ctggatatcc aaaagcaaat ggatgcaatg ctcgccacca tgtacgccgc gccaggcatt
     5461 ggtctagcgg ccaatcaggt caatcttctt aaccgcgtac tggtcatgga tctggcccgc
     5521 accgacgaag gcgaagaaaa aacgcctatc tgcatggcca accctgaaat catctgggaa
     5581 tctgaggaaa tgagcacgat ggaggaaggc tgcctttcaa ttccgcggca atatgcggaa
     5641 gtgcagcgcc ccgcccatgt ccgtgttaaa tatctggatt acaatggcaa agaagccgag
     5701 ttggaagctt ccggtctgct ctcccattgt gtccagcatg aaattgacca cctcaacggt
     5761 gtgcttttta ttgattatct ctctacgctc aagcgtaaca tgatcgtacg caaggtcgaa
     5821 aaactcaaaa aacagactat tctttagcgt gcgatgcccc tttataatgc agcatgaaca
     5881 ggaaataccg accagtcggt cttttttgcg gttggtgata taaaaaggca acatgggctg
     5941 tttttgcaaa atccgtattt ataatcgccg cttctgtgct aaaagcgtca catgataaat
     6001 gaaaccaaac ccttacgcgt aggctttatg ggcaccccgg actttgcggc cagcgcactt
     6061 aagaccatcg atgccagcgc gcatgatgtg gtgtgcgtct atacccagcc gccccgcccc
     6121 aaagggcgtg ggcatagact gcagccctct ccagtgcatc aatatgccga tgagcgcggc
     6181 attcccgtat ttcatcctaa atctctgaaa tctgctgacg tacaggccga gttcacagcg
     6241 cataatttag acgtggccat tgtcgcagcc tatggcctta ttctgcctaa agctattttg
     6301 aatgcgccga aatatggctg catcaatatc catgcgtcgc ttttaccgcg ctggcggggg
     6361 gcctctccga ttcagcgcgc aatctgggcg ggtgataaac aaagcggcgt taccctgatt
     6421 cagatgaatg aagggcttga taccggcccg gaaatcatga aaaccgctct ggatttagac
     6481 ctgcgcgaaa ccaccaccag cctgcatgat cgcctcgccg cgctgggcgc tgatatggtt
     6541 attgaaacgc ttgataaatt ggctataaaa ggccatttaa ctgcaaaaga acaggaaaat
     6601 tcaggtgttg aatatgcgca tttgctcaaa aaagacgatg gccgggttga ttggcctcaa
     6661 agcgcgactc aaattgatcg ccaaatccgc gccctcaatc cctggcccgg tgtctggacc
     6721 aagaccgcag atggtcaacg cctcaaaatc ctcaccgcac aacctgcaga cgcctccccc
     6781 ctcgatacaa cgcaggcaga aaaaaaccga ccggtcggta tgttaatcga taaaaccggc
     6841 catgtccaat gcgggcaggg cacaatcttg tgtctccagc gcgttcagcc cgctgggaaa
     6901 caggccatgg atcttcctgc cgccctcaat ggtggttacc tcaaaattgg cgaggtcctc
     6961 gcatgagcac gacctcttac ggcctcattg ctctggtgat gtttactatc ggcaatattc
     7021 tggtcgtgct gaccaatgcg attcccggtt tgcaaatggc ggcgatgtca cttttccttg
     7081 gcgcgttggg catgaccctt taccaagccg tgaagggaca acccatccgc acctattggc
     7141 gtcagccgcc aagacactat gcctttttgg tcagcggcgt tggtgtttac accgcgctga
     7201 tttatgtggc cttccgccat gcgccgcctt ttgaagtcaa tgccattaac tatctctggc
     7261 cgatccttct ggttgctttt gccaccctgt ttcatactgg caggctcaac gcacctaaaa
     7321 ttgttggcat tgtcatgggc tttatcggca catttcttat ttttgtccct gcacacgcaa
     7381 acgggtttat gggcggtcta caagcaggac attttttagc cctgattgcg gctatcctgt
     7441 gggcggccta ttctgcttcg gctaaaaaca aaacctatcc tgccggatta atggccccag
     7501 ccatgtttat ctcggctttt ttgtgtgcag gcctgcattt gatgtttgaa aaaacagtaa
     7561 tgccggtggg gatagagtgg gtgtatgtgc ttttgctagg tttttttcgc ctatcctatt
     7621 tgttctggga tgaatccatg cgtggcggta atgttgtttt tttagcgtct ttggcatatt
     7681 ttgtaccgct agcctcaact ctgcttttta ttgcattcgg ctatacggca gccaacattt
     7741 ggattgcgct gggtgcgagc ttcatcgtat caggttgcat cattgtcaat ttacgagacc
     7801 ttatgaggct gatcaaacga tgactcagcg ctaccgcctt acgatcgaat ataacggcgc
     7861 agattacgcc ggttggcaac gccaggaaaa ggccggccca caaacgattc agggcgaaat
     7921 tgaaaaagcc atcaaggctt tttccggcca agatgtgacc gttcaatcgt ccgggcgtac
     7981 ggatgcaggt gttcatgcgt acgggcaaat cgctcatgtc gatctggagg acctcacccg
     8041 gcccatgcaa ccttttgaaa ttgccaaggc aatcaacgcc catctgtacc cccagccgat
     8101 ctgtatttta tctgcagagc aggtatccga tgatttccac gcacgcttta gcgctaaaaa
     8161 caaacgctat atttatcgta tcttaaaccg tcaggctttt ccggccatcg agcgcggttt
     8221 tgtctggcat atccggcgtc cacttgatgt ccccgcaatg caagaagcgg cgcaagtact
     8281 gctcggccat cacgatttta ccacctttcg cgattccgaa tgtcaggcca aaaccgcgat
     8341 gcgtaccctt gatcgccttg aggtgagcgc acgcgaatac gatactggcg gaggcatcga
     8401 aatccgtatt gcggctgagg ctaaatcctt tctccaccat atggtgcgta accttgctgg
     8461 cacgctcagt ctcgtcggtg agggaaaatg gagcgctgca gatgtggctg cggcattaga
     8521 agccaaagat cgctgcgcag gcgggccaac tgctccggcg tccgggttat tccttgaaag
     8581 ggttgattat gagtaagctg gtaaaatcca aacgcgttgt cactttcgtt ccggcggatc
     8641 acaccaatga atttgccaag tctatggctg catatatccc gcatctgttt ggctgctacg
     8701 attccgtatg ctggtggggc acaccaaaaa cggaaaaagg catggagcaa taccgtccgc
     8761 tggataggga gatcgaacaa accccatccg ttcgcatgga attttccatc cctgatgacc
     8821 ctgccgctct tgaaaatttc accgccgccc tcaaaaagca tcacccctgg caagaacctg
     8881 taattctgat attcgatagc cagattattg acggctgaat agattatgta aaaaataatt
     8941 ggacttttga gtctgcctgt tttatacatc agcgcaagga gacacatacc atgcgtttaa
     9001 ttttttgccc cctgatgata atgtttgcta taagctttgc tgcacctgcc acatttgctc
     9061 aggatctcaa aaccacacgc gcatgggaac agctcgaaaa aatcgagaaa tcacgcccat
     9121 tgcgcaactc cagatatatt tcctcaaaag cggttatctc tgatcgggtt ctggatagaa
     9181 ccaaccgtgt aatcggtgaa gtccgtgatg ttgtgttgga ccgcaacggc gcgatcttct
     9241 ctctcaatat cgattttaac cgcttgcggt taggaacagg cccgatactg gttgattata
     9301 atgatatggg cctgcgcccg gccagcaacg gttataaaac gagctacacc gacgacgaaa
     9361 tccgcgtacg cctaccacaa attttggctg gtcttgaaag cgcatccgga caggaaacag
     9421 atacgtacag tatcaaaaat ttggtcggtc gtacattcta cagcgccgat ggtcgtaaac
     9481 ttggcaccat taaagacgtc ttatttgatc gtaacggtta tcgcgctgaa ttgctgtatg
     9541 ttatgttttc acataaaact gctcgcggta aatcgctagc tataccgttt cgtgaggcgt
     9601 actacgaagc aggtaagctc acccttaaaa atgactacgc aaagaccata ttggcttttg
     9661 ctcaggaaaa ataacgctcc aacacacggc cataaatttt caccagatcc ttaagatctg
     9721 ataccttcgc attttcatca atctggtgaa tgctttcatt aatgccgccg cactcaatca
     9781 caggacaata attgacaata aaccgcgcat ctgacgtacc gccgccggtc gtacattccg
     9841 gtttaaatcc ggtaacatct tcaaccgccc caatcaatat atccgaccac gcaccaggct
     9901 gcgtaataaa gctttcagca ttgccttcaa attcaatttt ataatcaaac cccgtagaat
     9961 ctagtaattc cgtgattttg gtttttaaac tagcgctcga ccagtgatca ttgaagcgaa
    10021 tattaaatgt tgcccatcca tgctcaggaa tgacattggt cgctttattt ccgacatcaa
    10081 tagtcgtaat ttccaaattg gtcggtggaa aaaactccga accttcatca aaagtatggc
    10141 gggctaatat attaagcagc gttaccagcc gtggcatcgg attatttgct agatgttggt
    10201 aggctacatg accctgcttt cccttgacgg tgagatggcc attcagggac cctctgcgcc
    10261 ccaccttgat ctcttgtcct aaatgttcgg gattggtggg ttcgccgacc aagcagacat
    10321 ccggcacatg tccatttttc tccatccact ccagcacctt taccgtaccg tctacggcca
    10381 aatcttcttc atccccggtg atcagcatgc tgatcgaacc tttaagatcg ttattcttaa
    10441 ggtattccct cgccgccaca gcaaacgccg caaccgagcc tttcatatcg ctggccccac
    10501 gcccataaag tacgccgtct ttcacttccg ggttaaacgg accgaatgtc cattggcctt
    10561 ccggccccgg cggcacaaca tccgtatgcc cgccaaagca cagatgaggg ccttcagagc
    10621 caatgcgcgc aaacagattg ggcacttcat acccttcctc ggcaaaaggc aaatgatggc
    10681 attcaaaccc cagctcttcc agaaaattag ccagaagtac ttgtgcccct gcatcctctg
    10741 gcgtcactga gggacaagca atcaattttt gtgtgagttt aacaacgtcc ataaatactt
    10801 cctgtctgga ttattgttta gaaatgaatc caatcaatcc cgcagcagat cgttaatact
    10861 ggttttagaa cgcgtttgcg catctacccg cttaacaatc accgcgcatg ccagatttgg
    10921 ccccggcgtt ccgtcgggta aaggcttgcc cggcatcgtc cccggcacca ccacagaata
    10981 agccgggaca cggcctcgga aaacttcacc ggtttcgcgg tcaataatct ttgtggatgc
    11041 gctgataaac acgcccatgg aaagcacaga gccttcttca acaatcacgc cttcaaccac
    11101 ctcggaacgc gcgccgataa agcaattatc ctcaataatc gtcggtgcgg cctgcaaggg
    11161 ctccaaaacg ccgccaatgc caacgccgcc ggacagatgt acattcttgc cgatctgcgc
    11221 gcaagaaccg actgtcaccc atgtatcaac catcgtgcct tcatcaacaa acaccccaac
    11281 attcacaaaa ctgggcatca acactgcgcc cttggcgata tgggcactgt aacgcaccac
    11341 gcagtttggg accgcacgaa aacctgcttt ttcaaattcc gccttatccc agccatcaaa
    11401 cttgctggcc accttgtcat accaacccga accatcaggt ccgccggaaa ttttttgcat
    11461 ggggttaaga cggaatgaca acaagacggc ttttttcagc cattgattga cctgccagcc
    11521 ctcacttgtt ttttctgcca cgcgcagcgc acccgtatcc agcagcgcca acgcttgctt
    11581 catggcctcg cgcatttctc cacttgtgtc tgggctgagc gtatcacgcg catcccatgc
    11641 gctttcaatc gcggtttcaa gatcggtatg ttgtgctgca gcctggccca tatgtctgta
    11701 tccttgcata agtaacgaat atggcctgca cttgaccacg aagtgaataa aaaatcaaga
    11761 tgaaggcgga tttcgccaaa agggcatgca tattttcagg tgaaaagaag ctttttcgca
    11821 cgctccagtt ttttaatttc cccgcgcttg agatagcgct taacaggctc cagtaactga
    11881 acaaccaacc gataattttt ttcacggtaa gctttagcgg catgctgaca cgttgcatcg
    11941 atactgatct tatgtttttc acgaatgctt tgttccaact gcttatggcg aatggtcatc
    12001 gcgcggttta acagcttatc gccaggttct agtaaaattc ggctgtgttt ttcaaagctc
    12061 tgtgccatct cacgaattgt ttttttaata agactgacat cactaacgcc ccaatcgcca
    12121 cgcaacgccg tatcagattt ccgtgccgct gtcataattt cgctgagttc aaatctatca
    12181 attgggttat aaaacgcatg ggcttctata agaaactgga tactgctata ggtgatttca
    12241 aatgcctgtt cacccttttc atagcgagtt acggccataa acggcccatt aaattcttgt
    12301 tggttaaggg gtttgatcat cttataatta ttgcgatatt ccagaagacc gcaaaaaaac
    12361 atgtagccgt agcgctgttc taaaaacctg aaatgccgct cgcattcgga aaaaaagcac
    12421 tgcaatagct ctccgttgag tcttatatct cctgataccc ctgttttact cataccgctt
    12481 acgtacacac ccctaaaaat aattcctaaa ttaacctgca atgccttatc atcagggcga
    12541 aaatttgtta ttgaaaaagt atgaaaccgc cccatttaca gtgccagatt aactggatac
    12601 ctcaatttac taatataagg aaagaacatt tcggaaaagt taaaaattcc aatatcttac
    12661 aagctctttc gtataggtct gccctagagc ccacatcttt gcagatctca aaagcggcgc
    12721 tggtattgat tgatgggcac aaagcgggct atctgcaaac tgttgaaacc cgcatagcca
    12781 agggcttcct tcactttata gtgcttgatc gcggaccatt atggtttgat ggttttggta
    12841 gcgcagagca tttcgacgct ttttgtacgg ctttgcgccg ggaatttccc cgcagaccag
    12901 gccgatttat gcgttttatt cccgaaatcg aagactccaa agaaattcaa aacattatga
    12961 aagcgcacga ctttcgtaaa aaagcacccg gttaccaaac agtttggctg gatttgaaca
    13021 acgatgaaga aactttgcgg acaaatttaa aaagcaattg gcgcggccat cttcgccaag
    13081 cggagaagaa cggtttgagt gtggaatggg acgtggaagg ttgttaccta gactggatac
    13141 tccggggtta tgcgcgggat aaatcaacga agggttatcc cggtccatcc gttcaattta
    13201 tgaaaaggct tgctgagata tgtataaagg aaaaggaact cttgataggc cgagcgttgc
    13261 tggatggaaa gcccattgcc ggggtgttga ttctgtttca tggtcaaagc gcgacctatc
    13321 aaatcggttt caccagcaaa ctgggccggg aaaaatcagc gcataatttc ttattgtggc
    13381 aaagcttgct atacttaaaa gagaatggtt tttcagcgtt cgatcttgga ggcgtaaacg
    13441 atgataccgc acaaggcgtt aaaaaattta agactgggct tggcggccgc tgcattactc
    13501 tggctggtct ctacggctga tccggcttcc gctggcgaaa cgctgcgcca gcaattggtc
    13561 tatgaagtct atgccggtgg tattcacgct gtgcaggcgc acatggatat cgatttatct
    13621 aaaaagaata aatacagtct ggttctcgac gccaaaaccc gtggatttct aggctcgtta
    13681 attccatggg aaggctcttt cgaaagttac ggctggatac tcaaagacgg caaatatcag
    13741 cccgaaagac accaatcaac ggcgacttgg cgtaacgaag ccgatataaa agaatatctt
    13801 tataacaaaa atggctcctt taaatccctg cgcatcaccg atgagcatag cgccgccgag
    13861 ttgcgtgaag tcgatgacga agtcaccaac ggcactgttg atattttgag cgcaacattg
    13921 caggttttgg atgcctataa tgaaatggag acatgcgctg gagaatcgga aatattcgat
    13981 ggtaaacgcc gttttaaaca gatattcagg catgaagagg ccgtaaccct tacagaatca
    14041 aaatataata tttatggcgg tccggcagca aagtgcacag tcgaggtcgt acccgtaaac
    14101 ggtgaatggc acaaaaagcc acggggctgg atgtcgattc aggaacaggg ccgcgcaaaa
    14161 ggcacgatgc cgaccgtctg gatcgcccag gttgctgaga acggcccggc cgttccggta
    14221 aaaattcgtg tcaaaaccgc ttatggcaca ctgtttatgc acttggctga atataaatcc
    14281 gccgataccg tcctcatcgc tgaaaaacgc atgcccgaag acgaataacc cgcaatattt
    14341 acttgtattt gcggtgtgtt tggcgacaat agcctctatg agcacaaaac cactcgcgat
    14401 tgttattctt gcggcgggct tcggcacacg catgaaatcc tctcaagcca aagtcatgca
    14461 tacattggca ggcaagccga tgataaattg gctgctggaa tcttgtgaaa gcctgaagcc
    14521 tgagaaagtt gtagtcgtca tcggcccgga gatgccggat cttgaaaagg ctgtcaaacc
    14581 ccatatctgt gttgtgcaga aagttcgcga tggcactggc ggtgctgtgc gcacggcaat
    14641 gcctgcgctc gaaggcttcg acggcgatgt cctgattttg cttggcgaca cgccacttat
    14701 tactcccgca acactctccg ccctgcgcgt tgccaaaaac gatcacgcgc tggcaattct
    14761 gggttgcatc atggataatc ctcacggcta tggtcgcctg attctagggc aggaaggtat
    14821 gctcgaaaac atcgtcgagg aaaaagacgc cgatcctgaa caaaaagcaa tccggctcgt
    14881 caataccggc gctttttgcg ttgatggagc gcagcttagc ggatggcttg atcagctcga
    14941 ttccaataac gcccaaaacg aattttatat cacgcagctt ccagccatta ttcgtgagca
    15001 aggcggtgta actgccgccg ccgtcatcga aaatcccgcc gaagtaaaag ggtgcaacac
    15061 ccgcactgat ctcgcggcgc tggaaaaaac gctgcaaacg cgattgcgtg aagacctgat
    15121 gaatgaaggc gtatatatgc aagcgcccga tacagtgtat ctctggcatg acaccaaaat
    15181 tgctccggat tgtattattg aacccaatgt ttatttcggt cccggcgtga cgctggaatc
    15241 cggcgttcat ataaaagcgt tcagctatct cgaaggtgcg catgtgaaag cgggcgcatc
    15301 tgttggacca ttcgcacgcc tgcgacctga tacaaaaatt ggcaagaatg cgcgcatcgg
    15361 caattttgtc gaaattaaaa aatcaactat cggtccaggc agcaaaatcg gccatctcgc
    15421 ctatgtcgga gacacggata tgggagaaga agtcaacttc tctgccggcg cgattaccgt
    15481 gaattacgat ggcttccaaa aacaccggac cgtgattggc aaaaacgtta tggtcggctc
    15541 gaacgtcaat ctggttgcac ctgtgcgcat tgatgacggt gccttcgttg ccgccggttc
    15601 gaccatcacc gaagatgtgc ccgccaatgc gctatccatc gcccgcgatc cggccgaaat
    15661 ccggcagggc tgggctgcca aataccgtaa gatcaaggaa gcagcacaaa aaaaagcaaa
    15721 aaaacagcgt aaaaaagctt cctgatttta ttgtgggcag tagtccgaac ggccgataaa
    15781 ttttttaaat aattctatgg aagcctgtac tctgaagtgg ttatgcgctt tttctttttg
    15841 acaatatttt tgtcggttat tcctttctcc gttcacgcga cctgcgaggg ctataacaac
    15901 gccatgaatg cactggaagc ggcgattgct gaaagttacc gccccaagat gcaaccgccg
    15961 ctaaaatacc gcgatcccga taatgcgcca cccgtggcgt ctatcaatgc agtgattgat
    16021 gatctggaaa ttgcaaccca aaagctgggt gcgcccaaag cacctgtgcc agcccaatcc
    16081 tatccccgcc gcccaaaacc tcatctgtaa catcccgcaa catcatgtga cttgcggttt
    16141 taagaagaat gattaaattc ttgatatact cgttttgctt tgtggttgta caaagcgcta
    16201 ataatgatgt gcaaacacaa atgaaaataa ttagatatgt gcggaataat tggaatttta
    16261 agtgaagaca gtgcagcacc ccttttgatt gagggtttac gccgcctcga atatcgcggc
    16321 tatgatagcg ccggaatcgc cacgctgcat gacggcgtgc ttgatagacg ccgttccgag
    16381 ggcaaactta aaaacctcga aatcaaactc aaaagcagac cgcttaaagg tacgctcggc
    16441 attggccata cgcgctgggc gacccatggc ggcccgaccg aggataatgc ccacccgcac
    16501 gcgaccgata aagtcgcagt cgtccataac ggcatcattg aaaattatgc gcagttaaaa
    16561 gaagaactgg ccaaccacca aatccgcttt gaaaccgata ccgataccga agttattgtc
    16621 catcttgttg atcattatct gacccaaggt aaaagcccac aagaggcggc cagcaccgcg
    16681 tttgatcgcc ttgacggggc ttatgccatc gtgatgattt ttgccggtca ggataatctt
    16741 atgatcggtt gccgtcaggg cacaccgctg gctgtcggct atggcgacgg cgaaatgttt
    16801 gtcgccagcg attcttatgc tcttgcgcca ttaaccaaaa aaatctgctt tcttgaagat
    16861 ggcgaccgcg tcaccgtcac ccgcgaaggt gcgcagattt ataccaacga tgacacgccc
    16921 gttacccgcg aaatccgtat cactgcccaa agcggtgcga cgaccggaaa aggcaatttc
    16981 cgccacttca tgctcaagga aatttatgaa caacccagcg ttattggcga tacgctgaat
    17041 tctttcgtca atccggcaac gggtgaaata tcaattcctc ctgaggtgct cgctgctctt
    17101 gctgacgccc cgcgcattac catggtcgcc tgcggcacgg cctattatgc ttgcatggtt
    17161 gccaaatact ggtttgaaca gaccgtaggc attccctgcg atgtcgatat tgcctccgaa
    17221 ttccgctacc gcgaaccacc gctggcttct gatacacttt gcatcgtcgt ttcccagtca
    17281 ggagaaacgc tggacacttt agaaggcatg cgctacgcaa aatctgaagg atgcaaagtg
    17341 ctttccatcg tcaacaccat cgaaagcaca atcgagcggg aatccgactt cgtcatgcac
    17401 acgctggccg gaccggaaat cggcgttgcc tcaaccaaag ccttcactac ccagctcaca
    17461 acactggcct gcatcgcact ttcactggca gtaaaaaagg gcgcgttgaa acatgaaaaa
    17521 gccgccgtgc tcgccgatga gctgcgtcat atcccgcagc aaatcaccca gctgttacgc
    17581 gaggaagaaa acaccgaaat catacaatcc attgcccgtg atatcttcga aaacactgac
    17641 atgctttatc taggtcggaa tttgctgtat ccactggcca tggaaggcgc gttgaaactg
    17701 aaagaagttt cctacatcca cgccgaaggc tacgcagccg gagaattgaa gcatggcccc
    17761 atcgctctga tcgacgacca tatgccgatc gttgtgctgg ctccggcggg tgatgccttg
    17821 ttcgaaaaaa ccgccagtaa cttacaggaa aatgtcgcgc gcggcgccaa agtattattg
    17881 atctccgatg aatcagggca cgccaaactc aaagagctca tccactgggg cattaaacta
    17941 caacccctcg atccgttcg
//
LOCUS       DAOSWR010000027        17896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2002_length_17896_cov_5.409282, whole genome shotgun sequence.
ACCESSION   DAOSWR010000027 DAOSWR010000000
VERSION     DAOSWR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17896
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2002_length_17896_cov_5.409282"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK513_03400"
     CDS             complement(<1..435)
                     /locus_tag="PK513_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO81530.1"
                     /translation="MNTTLQTMRKDEQGFTLVELAVVMIIIGLLIGGILKGQELITNA
                     RVTSTASQMESLGAAYNTFRDQFNAIPGDMGSATARLANCPNTSACGIDISATANNGI
                     IDENVGEAPADEGIAFFGQMLAAGLITGMDGSGQTAAMGVTNP"
     gene            complement(660..1736)
                     /locus_tag="PK513_03405"
     CDS             complement(660..1736)
                     /locus_tag="PK513_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81531.1"
                     /translation="MNQISFSLPEILALIGVVQCTYLIVHITLRSGMVLRAGLPLVYF
                     LVLAAAFTADLAQNRLQFEGDYYFLMQWFLWFSGPPLSVLLVVQLSDMNTTPPLRDYW
                     VLLLLPLSFMLSVLSAGSAIGCEDFKNCEAMHELLKVTGLMAGTISLLVIFSKKQLMK
                     TLHKQKFGKERYWLIFSLIALNTLFLLTMLLSLTQYISPDEALMLRNILGIGFAYLVS
                     TSLLRIIPRNQGPAAQTGASAENLSEEERDLAEKIEGLLKFEKVYQEPTYSRADLARE
                     CETSETIISRVINAHFQKSFPQLMNERRIEDAKQLLRETDATIQAIAKDVGFNSLPSF
                     NRVFKELANQAPSQYRKKTPQNTN"
     gene            complement(1776..2522)
                     /locus_tag="PK513_03410"
     CDS             complement(1776..2522)
                     /locus_tag="PK513_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81532.1"
                     /translation="MLRVSQHNAALASLFVLFTLSIALNIALWSSVRSIQTRWSNVPP
                     TPGLSGALWSSLGDPQLAYRGYGLMIQNMGDTGGRIINLGEYDYNALGRWFRLQHQLD
                     PHSSFTPFVAGYFFGGLRSRPEKIAAIVDYLELAAGDGSGEKWRFMSHAVFLARYRMN
                     DMNRALELAQKLAAYDNPDMAVWARQMPIFILTAQGERQAAYEMMVNLLKTEGANLHP
                     NEVNATLAYICEQILSPEEAALDPLCKDYK"
     gene            complement(2525..3304)
                     /locus_tag="PK513_03415"
     CDS             complement(2525..3304)
                     /locus_tag="PK513_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81533.1"
                     /translation="MKSLPLIGPSLVGYVITAAIRDKLIIGICVLLAAAVSLSVFLGS
                     AAITEQDQFVAVFSAGSVRILNIVGLVLFIVFFIRRSFETRDIEFMLSRPVGRTQLVL
                     SYAAGFSLIATLMGVISGLCIMALSPHLVSGGHALWILSLVVENVIMVNVALFFSMIL
                     TSAASCTFATFGFYVLARMMSQILGIIDSGKNAVDLKILEMIMQAISSVMPRLDLLTQ
                     TSWLVYGPDENIGPAFILMQGIIYSSLILLAALIDMRRRQF"
     gene            complement(3315..5144)
                     /locus_tag="PK513_03420"
     CDS             complement(3315..5144)
                     /locus_tag="PK513_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system family protein"
                     /protein_id="HOO81534.1"
                     /translation="MIVFHPVQNRFSFTVLSTCCVLFSSFFLTSCDMAKNQLKADREG
                     SMEMQDYRDGLATRLPDTDEGRADQGAIPDLQPYVAQSSERMKPMPLVSIKINQSVPL
                     RDALYELAQQADYDIELDPRIRGSIIFTATNRPFDVVIERIADIAGLRYKFEDDILRV
                     ELDTPYNSVYKIDYLSYIRNSKSSISNNVSVVSGDGTDSGSNFEADSESEANFWGELE
                     VNLEQIVRGNQTGALKTKRDPRITAAEQNPDVQAVAPTSEDGTQVAVQPPQATLRVES
                     LPVDDDDDTSSSRGKKDEPEGTFTINKQAGLINVYASEKAHKEIKEYLKLLKRAVTAQ
                     VLIEAKVLEVTLSDEYRTGIDWDIVGLPGEFSLDFASGSNIGTPLLTAAYNGNDIQSL
                     ITATSRFGTVRALASPRLTVLNNQPAVLNVATNRVFFEIDIDVTQDGVTAQTEVNTEI
                     RNVPEGVLVNVQPSINLEDRTISMAVRPTVTSIVSEEPDPGIAYIIAQCGAPCNNLED
                     SLVPELNVQEIDSVVKVRSGQAIVMGGLLQDRAQVTDGGVPILGEAPIIGNLFKDHDD
                     SVSKTELVIFLKATILDNPSDSVHNTDKDLYRQFSSDRRPLRF"
     gene            complement(5261..6412)
                     /locus_tag="PK513_03425"
     CDS             complement(5261..6412)
                     /locus_tag="PK513_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOO81535.1"
                     /translation="MTPPETPPAILNYLLSMGQHEASDLYLTVGQPPTLRAEDKMIYL
                     SERKLVLEDIDAILDSILTARQKREFENKMELNTALDMGEQGRFRINVMKQRQFPALV
                     IRRIVSKIPSFKDLNLPPILERLAMEKRGLVLVTGITGSGKSTTLASMVDYRNERSEG
                     HIITVEDPIEYYHEHKKSVVTQREVGVDTESFSIAMKNSLRQRPDVILVGEIRDRDVM
                     EQALTITETGHLCLATIHTNNAYQAIERIVNFFPEELAAQVRLNLAMNLKAIVSQRLI
                     MGVNQKMVPAIEIMLNQGLVRELILKGEIAKIKDVMEQNTTLGMCTFDQCLMDLYVRG
                     LITEETAMSNSDRPSDLKIKLHQHQLSTKNNKGEKGLTQIDTSRIKFGD"
     gene            complement(6409..7551)
                     /locus_tag="PK513_03430"
     CDS             complement(6409..7551)
                     /locus_tag="PK513_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOO81536.1"
                     /translation="MDLTQLLAFTMQNDASDLHLSAGSPPIIRVSGLLKRVKADPLSS
                     DDIRTMLYSVMTEDQRAEYEKNMEIDFAIALGEKARFRVNGFTSRLGASAVFRTIPTE
                     VPTMEQLGLPPVMRRFSELEKGIVLVTGPTGSGKSTTLASMVNHINLTMSKHILTIED
                     PVEFFHTSQKSLVNHREVGTDTKSFARALKSALREDPDVILVGEMRDYETISLALTAA
                     ETGHLVFATLHSNSASKTIDRVIDVFPTGDKEMVRAMLASSLQGVIAQTLVRKADGQG
                     RVGAFEILVGTNAVRNLIRENQIPQMYSMMQTGSRYGMVTMQDAIADLFEAGLIDKDE
                     ARRATLLTTNDDGDDDNEYAAAALGGAKLDESAAGGSGSKDGGYSF"
     gene            complement(7677..8150)
                     /locus_tag="PK513_03435"
     CDS             complement(7677..8150)
                     /locus_tag="PK513_03435"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO81537.1"
                     /translation="MMQSFIVLAMLPFLLALLMIDIRHKTLPNILVAIIFMLGLTSLA
                     YQVYLEPGTMKGRIVEHLGGALVYMTLAWLLAAGMTKILKKQALGMGDVKFFAVAGLW
                     LGLGDLGIFCILGGIFGVLFGVAWQKIKNEALFPFGPALIVSFFILLLMGGSHSL"
     gene            complement(8496..9554)
                     /locus_tag="PK513_03440"
     CDS             complement(8496..9554)
                     /locus_tag="PK513_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO81538.1"
                     /translation="MMWRFQIPSHFKLKTYSLGVCFIAGVGLSPFIAHAQSARQVLDR
                     AQELSVEMNAQEEAVIQDLLIQKNAASDVKQETDIQEITSPAAPLEEQDLMSATEAAE
                     VPALPEGQTEDGGEFDEDLFFDAEAYVPTSDLSRQGAPSKVNPALNPASRLVITRKKF
                     DADSKEARLVAAERATKLGRYESALEIYDGLYATNKRDPNILLGRATSFQRAGRFDEA
                     IAAYEELLEIRPSNVEAQINLQGLMGRRYPAVALINLQKLHEDNPGSTAIIAQIAVIE
                     AQLGRYPDAIRYLGMAASMEPNNANHIFNMAVIADRAGDKKQAVRYYEEALEVDTLYG
                     ASKSIPRETVFERLAQLR"
     gene            complement(9644..10402)
                     /locus_tag="PK513_03445"
     CDS             complement(9644..10402)
                     /locus_tag="PK513_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO81539.1"
                     /translation="MSTAKVQQQKPEDALSIKNVGVSYGAFKAIQDVDLNIKASECFG
                     LIGLNGAGKTTLIKAILDLRTPSDGEILIFNQRAGTKAVKQRLAFLPERFEPPWFLTG
                     LEFLKFSARMYKTSISNAQCIEYAERIALDPAALDRRVHTYSKGMRQKLGVLATILTD
                     CDLLILDEPMSGLDPVARALVKTLIVEARQKGCTVFLSSHILADMDEICDRVAVMHDR
                     SIRFTGKPDKLKALSGDLSLEQAFLHFIEQKSAA"
     gene            complement(10416..10985)
                     /locus_tag="PK513_03450"
     CDS             complement(10416..10985)
                     /locus_tag="PK513_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81540.1"
                     /translation="MRYYILLLLGIVVMSFGFAVMPRAQSVQRETPTAATETLSENQT
                     SADKDANSKKGFDPLQYDSILFTHWEHVAIQDARRYVGDTRNVTDSELAKDLKQREDI
                     GEKIKPPPEEREIRLSGIVFHGKADWAIWLNEERITPDAIPKEVMDLRVYKEYIEFKW
                     FDEYTNQIFPIRLRPHQRFNIDTRIFLPG"
     gene            complement(10985..11626)
                     /locus_tag="PK513_03455"
     CDS             complement(10985..11626)
                     /locus_tag="PK513_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81541.1"
                     /translation="MIAVLGIKRIFVLAALLLLNAFLAAGLYLYFIPEIQVKERELRG
                     LRGTVSTLRTDINRMQVEFEQLEDQKEEFEKLVRRGFFKDQSRRQAEKMFNVIQKNSG
                     VSKAIASVKSGSIEDNPEAQKAEHKILRSPVEIRLEAFDDVNIFHYLFLAENYFPGHL
                     SVEKISLKREADVTGTVLRAIANGKNPSLVGAQVELMWRTMIPEASVINGGGK"
     gene            complement(11623..13230)
                     /locus_tag="PK513_03460"
     CDS             complement(11623..13230)
                     /locus_tag="PK513_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81542.1"
                     /translation="MSLSFLAPNRTVLLVSDEFLSIYTVRGPGVKLIDTIPWEAENFE
                     KNVASIIAKDCGKKPVLILNDMVEQHYRKERVVRTGVGMVDKASMVKRKLNVAFPHYP
                     IRAAFPLKEKVAKTDSQPAADIYIFAAVAGNDQITKTIESTKKSMASVAAFCLLPIES
                     SDMVKALSGKIGAKKGRKKNVWSIFIGQHQNGSLRQVLTKNGELALTRMSPIAEKDDD
                     PQVWASELYQEFKSTISYLSRFGYDPADGLDVFVIANPAPAEALRSIFDEDYDLHILG
                     VDDAARALGVNIGRQADMRYADVLHVAWAGRKGSFVLPMKATQVDEVSKPRQVAMLVG
                     MALLCGSAFLGYQVVSSAGSLASLVSDIEGLKRNKSQLDAQYQREVQRLDKVGFDARL
                     VQGALAVHKEFEDDRIPILTVFQKVGKALGKDLRIDSIEIKKVKPTIAQNVWSAVKNE
                     SQPRFETRMKMTYPSTANVDKGNKEVADLSVRIESLLPGYKVEVTKFLKDYEYTEGLI
                     VEAGDLEKQDIQQDFVAEILIRGPAKE"
     gene            complement(13237..14373)
                     /locus_tag="PK513_03465"
     CDS             complement(13237..14373)
                     /locus_tag="PK513_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO81543.1"
                     /translation="MDCSAQSTKSKGGFGQIGQKKIKLRDVIQLFVQLEQMQSAGVPL
                     LDALSDVRDSTEHDGLRDIMSEIFRDVSDGAALSEAMNKHPKVFKPLYISLVEAGEET
                     GDLTASYLQLIKYLKWVDVIQAKIRKATRYPIIVSVVVILTVVFMMSIVVPQIVGFLR
                     FLDLDLPWFTIALIATSDFMVEPQFHFFGVPVYGSIIVMVVPVIIFGTVKLLRHSSDA
                     IAYKLDSWWLNAPVVGPLIRKISIARYAQTFGALYASGINVISALENSRATVTNLMML
                     DAMESVEGHVKAGSALSEAFNASGEFPSLVVRMVKVGEESGNLTPVLDQVSDFYTHDV
                     NEAIEALIEMIQPTLTAILGLIIAWIAAGSLGPIYMNLGNFMDF"
     gene            complement(14520..16541)
                     /locus_tag="PK513_03470"
     CDS             complement(14520..16541)
                     /locus_tag="PK513_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO81544.1"
                     /translation="MIHRDDKEPIEGIIVKESKSVKATEAPISETFHGLVLEEDAEPV
                     QVEVIDQNLPNSENEKSAAQLPARFVPARENQTQQSQSQSQQQRQDTGHALKTYSRNI
                     TINESEEDDDLRRKGRIGDRMVEMGLISKDQLNVALQEKKITGKMLGEVMVDLGFIDE
                     ETLSAFIAQSSGVELFDPQNTIFDGEVLGLITKEEAAKHKVLPVSMSEHHIKVAMADP
                     YDIVAMDMLRRFIPKGLTVKPMVCTPKILSEAIDAAYGYASSVNAILQELEEGKEQKD
                     VASLDESEAFSHPIVRLVNALVFEAVKIGASDLHFEPEENFIRLRYRKDGVLFTAQIL
                     HKQHWSGISQRIKIMSGMNIADKLGAQDGRFGMHVGGREADFRVSSLPTVHGENIVLR
                     VLDQSESIMPMEKLGFSNHNRNLIRQAQSKPEGIIIVTGPTGSGKTTSLYSMLNEIND
                     VEVNIQTLEDPVENSLPMIRQTHVREGVLEFADGIKALLRQDPDIIFIGEIRDGETAG
                     MALKAAMTGHQVYTTLHTNDSFGAIPRLFDLGLKPGMVAGAIVAVFAQRLTRRLCPVC
                     KEAYQPDAQECEILGADPANMPTIYRARQGGCDSCSGSGYKGRTAVVEILTFDEEMDE
                     LLARGANKAELKAMAVKKGFKSMKDDGILKVLEGVTSLEALAKVVDIRR"
     gene            complement(16738..17862)
                     /locus_tag="PK513_03475"
     CDS             complement(16738..17862)
                     /locus_tag="PK513_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOO81545.1"
                     /translation="MSTNFGLAEYIWLDGSVPTRFVRSKTRVVNLGAKPKITEFPEWS
                     FDGSSTQQAAGNDSDCILQPVSYFKDPIRGEGNFLVMCEVNNPDGSPHESNSRAQLRE
                     VLDAGASKQDPWAGFEQEYTLFPQGHRTPLGWPSHGYPAPQGPYYCGVGAEEAFGREV
                     AETHAELCLDAGLMYYGMNAEVMPGQWEFQIGYRGDNSEDSGVLNMADQVWVARWLLH
                     RVGEEFDIAATLENKPVKGDWNGAGMHTNFSTSDTRDKSKGRAAIDAAVDALSKKHAE
                     HIVLYGAGLSERLTGLHETCDINTFKAGDADRGCSIRIPKPVAIKGYGYFEDRRPGAN
                     ADPYLVAARLCTTVCGVDESVMRFSSWPRRDKKITAVAAE"
BASE COUNT         4763 a         4507 c         3969 g         4657 t
ORIGIN      
        1 cgggttagtc acgcccatgg ccgccgtctg tccagaaccg tccatgccgg tgatcagacc
       61 cgccgcaagc atctggccga aaaaggcaat gccttcgtct gcaggcgcct cgccaacatt
      121 ctcatcaata attccgttat ttgcagtagc agaaatatct attccacaag cactggtgtt
      181 gggacagttg gcaagacgcg ccgttgcact tcccatatcg cccggaatcg cgttaaactg
      241 gtctctgaac gtgttataag ccgccccgag agattccatc tgcgaagccg tggatgttac
      301 acgggcgttg gtgatcagtt cctgaccttt caagatcccg ccgatcaaca gaccgatgat
      361 gatcatcacg accgcaagtt cgacgagggt aaagccctgc tcgtccttgc gcattgtttg
      421 aagtgttgtg ttcattcgta aaatctcctc aaaatttaaa aatggatata aaggttttag
      481 gtgggatgat ttgacttgag cttccccgat tcaaatcatg tgaaatagta tacacataat
      541 ccccccacaa aaacagtccc tatggtttca tgatcgcttc aatttgatga atattttatt
      601 aataatgcca gatacttaca ctcaaggcgc gttctgaaca tatcaaaaaa acaaagtgat
      661 caatttgtgt tttgaggagt ttttttgcgg tattggcttg gggcctgatt tgcaagctct
      721 ttgaagacgc gattgaagga tggcagggaa ttaaagccta catctttggc gatggcctga
      781 attgtggcat ccgtttcgcg caagagctgt tttgcgtctt cgatgcgccg ctcattcatc
      841 aattggggaa aagatttttg gaaatgtgcg ttgatgaccc gcgaaattat ggtttcggaa
      901 gtctcgcact ctcgcgcgag gtcagcgcgg gaataggttg gttcctggta tactttttca
      961 aattttaaca ggccttcaat cttttcggcc aaatcgcgtt cttcttcgct caaattttcc
     1021 gctgaagcgc cggtttgggc ggcggggccc tgattacgcg ggatgatgcg caaaaggctg
     1081 gtgctgacca gataggcaaa gccaatcccc aggatattgc gtaacattaa tgcttcatcg
     1141 ggggagatat attgagtcag acttagcagc attgtgagca aaaacagcgt attgagcgca
     1201 atgagcgaaa aaatgagcca gtagcgctct ttaccgaatt tttgcttgtg cagcgttttc
     1261 atgagctgtt ttttgctaaa aatcaccaaa aggctaatgg ttccggccat gaggcccgtg
     1321 actttcagta attcgtgcat ggcctcgcag tttttgaaat cttcacagcc gattgcgctg
     1381 ccggctgata aaaccgagag cataaagctt aaaggcagga gcaaaagcac ccaataatcg
     1441 cgcaagggtg gggttgtgtt catatcggag agttgaacga ccagaagaac gcttaacggc
     1501 gggccggaaa accataagaa ccactgcatg aggaaataat aatcgccctc aaattgcaga
     1561 cggttttgcg ccaaatccgc ggtgaaagcg gcggccagaa ccagaaaata cactaacggc
     1621 agaccggcgc gcaaaaccat gcccgagcgc aatgtgatat gtacgatcaa atatgtacat
     1681 tgcaccacgc cgatcagcgc gagtatttca ggtaatgaga agctaatctg gttcattttg
     1741 ttaaaataag aatacttaat tcagggcgcg ccctattatt tataatcttt gcaaagcgga
     1801 tctaatgcgg cctcttcggg gcttaaaatc tgttcgcaaa tataggccag tgtcgcattg
     1861 acctcgttgg gatgcaagtt cgcgccttcg gtttttaaga gatttaccat catttcatag
     1921 gcggcctgcc tttcgccctg agcggtcagg ataaaaatcg gcatctggcg cgcccaaacc
     1981 gccatatcgg gattatcata ggcggccaat ttttgcgcca gctcaagggc gcggttcata
     2041 tcgttcatgc ggtagcgggc cagaaagacg gcatggctca taaaacgcca tttttctccg
     2101 ctgccatcac cggcagccag ttccaggtaa tcgacgatag cagcaatttt ttctggtctg
     2161 ctgcgcagtc cgccaaagaa atatccggcg acaaagggcg taaagctgga gtgcgggtca
     2221 agctggtgtt gcaggcgaaa ccaacggccg agggcattat agtcatattc accaaggttg
     2281 ataatacgcc cgccggtatc gcccatattc tggatcatca ggccataacc gcgataggcc
     2341 agttgtggat cgccaagcga tgaccacagt gcgccggaca ggccgggcgt tggcgggacg
     2401 ttgctccagc gcgtttgtat gctccgcacc gatgaccaga gcgctatatt cagagcaatg
     2461 ctgagcgtga ataaaacaaa caaactcgct aatgccgcgt tatgttgtga aacgcgcagc
     2521 atgatcagaa ttgcctgcgg cgcatatcaa tgagcgccgc cagtagaatt agagatgaat
     2581 agattattcc ctgcatcaag atgaaggcag ggcctatgtt ttcatcaggt ccgtagacca
     2641 gccaggaggt ctgggttaac aagtccagcc gcggcataac ggatgaaatt gcctgcatga
     2701 tcatttcaag tattttcaga tcgacggcgt tcttaccgga atcgataatc cccaggattt
     2761 gcgacatcat gcgcgccagg acataaaaac caaaagtcgc aaaggtgcag ctcgcggcac
     2821 tggtcaaaat catggaaaaa aacaaggcga cattgaccat gattacattt tcgacgacca
     2881 gactgagtat ccagagtgcg tgaccaccag agaccaaatg cggggaaaga gccataatgc
     2941 acaggccgga gataacgccc ataagcgtgg caattaatga aaatccggcg gcatatgata
     3001 acacaagttg cgttcgtccg acggggcgcg aaagcataaa ctcgatgtct cgtgtttcaa
     3061 agctgcggcg aatgaaaaag acgatgaaca ggacaaggcc gacgatgttc agaatgcgga
     3121 ccgagccagc agagaagact gcaacgaatt gatcctgctc ggtaattgcc gcagagccga
     3181 ggaaaacgga cagggacacg gcggcggcca ggagcacaca aataccgatg atcagcttgt
     3241 cacggatcgc tgctgtgatc acatagccta ccaaggacgg tccgatcagg ggaagcgatt
     3301 tcatctacgc cgccttaaaa tctcaaagga cggcggtcag atgaaaactg acggtagaga
     3361 tccttatcgg tgttatgaac gctatcgctt gggttatcca agatggtggc tttgagaaag
     3421 atcaccagtt cggttttact aacgctgtca tcgtgatctt taaagagatt gccgataata
     3481 ggggcttctc caaggattgg cacaccacca tcggtaactt gagcacggtc ctgcagcaag
     3541 ccacccatca caatggcctg tccggaacga acttttacaa ctgaatcaat ctcctgtaca
     3601 ttgagctcag gaaccaggct atcttctaaa ttgttacaag gcgctccaca ttgcgctatt
     3661 atatatgcaa taccggggtc cggttcttca ctgactatac ttgttactgt tggacgaaca
     3721 gccatcgaga tcgtgcggtc ttcaagattg atggagggtt gaacattgac aagaacgcct
     3781 tccggaacat tgcggatttc agtattaacc tcagtttgtg cagtaacacc atcctgagta
     3841 acatcaatat caatttcaaa aaacacccga ttggtcgcca cattcaacac agcgggctgg
     3901 ttattcaaaa ctgtcagacg cggactggcc agggccctta cggtaccaaa ccgggaggtc
     3961 gcagtaatca gactctgaat atcattgcca ttataagccg ccgtaagtaa cggcgtacca
     4021 atattgcttc ctgaagcgaa gtccaagctg aactcaccgg gcagccctac aatatcccaa
     4081 tctatccctg ttctgtattc atccgacaag gtcacctcga gaactttggc ttcgatcaag
     4141 acctgtgctg tcactgcgcg tttcagaagt ttgagatatt ctttaatctc tttatgcgct
     4201 ttttcagagg cgtaaacatt gatcaggccg gcctgcttgt tgatggtaaa tgttccctcc
     4261 ggttcatctt ttttacctct tgaacttgat gtgtcgtcat catcatctac aggcagtgat
     4321 tcaacacgca gcgttgcttg cggcggctgc acggccactt gcgttccatc ttccgaagtt
     4381 ggggcaacgg cctgcacgtc cgggttctgc tcggcagccg tgatgcgcgg atcacgtttt
     4441 gtttttaacg cgccggtctg attgccacga acgatttgct caaggttgac ctcaagctcc
     4501 ccccagaaat tggcctcgct ttcagaatcg gcttcaaaat tagaaccaga atccgtccca
     4561 tcgccggaca caactgaaac attgttgctg atgctgcttt ttgagttacg gatatagctc
     4621 aaataatcaa ttttgtagac gctgttataa ggtgtatcga gttcaacgcg caagatatca
     4681 tcttcaaact tgtagcgtag cccggcaata tcggcgatgc gctcaattac gacgtcaaaa
     4741 ggcctgttgg ttgctgtgaa aatgatagag ccgcgaatgc gcgggtccag ttcaatatca
     4801 tagtcggctt gctgcgccaa ttcatataag gcatcgcgca gaggcacgga ttgattgatc
     4861 ttaattgaaa ccagaggcat gggtttcatt cgctcggatg actgtgctac ataaggctgc
     4921 aaatccggga ttgccccttg atcagcccgg ccttcatccg tatcaggcag gcgtgtggcc
     4981 aggccgtcgc ggtaatcctg catttccatg ctgccttcgc gatctgcttt aagctggttt
     5041 ttggccatat cgcaagacgt caaaaagaac gacgaaaata gcacgcaaca ggttgaaagg
     5101 acggtgaagg agaaacggtt ttgaaccgga tgaaaaacaa tcatggaccc tagcctcgta
     5161 tagaacggtg atgcacagtg cgaacgcacg ttgcgattct caaatagcat agcatatttt
     5221 gaagcaggat gtagctataa gtttaagtga gttgcggctt ttaatcacca aactttatgc
     5281 gcgaggtgtc aatttgcgtc agcccctttt cgcctttatt attcttagtg gataattggt
     5341 gttggtgtaa tttaatcttc agatcagatg ggcgatcaga gttagacatt gcggtttctt
     5401 ctgtgataag gccacgaaca tacagatcca ttaagcattg atcgaatgta cacataccca
     5461 gtgttgtgtt ttgctccatc acgtctttta tttttgcgat ttcacctttc aggatcagtt
     5521 cacgtaccag accctggttg agcataatct caatggcggg gaccattttt tgatttaccc
     5581 ccatgatcaa gcgttgagaa acaatcgctt tgaggttcat tgccaaattt aagcgtactt
     5641 gggcagcgag ttcttccgga aagaaattga cgatacgttc aatggcttga taagcgttat
     5701 ttgtgtgaat agtcgccaaa cataaatgcc ctgtttcagt aatcgttaaa gcctgttcca
     5761 ttacatcacg atcacgaatt tccccgacca gaattacgtc cgggcgttgg cgcaaagaat
     5821 ttttcattgc tatggaaaat gattcagtat ctacaccaac ttcacgctgc gtaacaacgg
     5881 actttttgtg ttcgtgataa tattcgatcg ggtcttcaac tgtgatgatg tgaccttcgc
     5941 tgcgctcatt acgataatcc accattgagg ccagcgtggt cgatttaccg gaacctgtga
     6001 tcccggttac cagcacaagc cccctttttt ccattgctaa ccgttccagg ataggtggta
     6061 agttcaaatc tttaaatgaa gggatttttg acacaatacg gcgaataacg agcgcaggaa
     6121 actggcgctg tttcataaca ttaatgcgga aacgcccttg ctcccccata tcaagtgctg
     6181 tatttaactc cattttattt tcaaactcac gcttttgacg tgccgtcagg atgctgtcaa
     6241 ggatagcgtc aatatcctcc agaaccagct tcctttcgct cagataaatc atcttgtctt
     6301 cggcacgcaa ggtcggaggc tgtccgactg taaggtataa gtcacttgcc tcatgctgcc
     6361 ccatagacag caaataatta agaatggccg gaggtgtctc tggaggtgtc aaaatgagta
     6421 ccccccgtct ttcgatccac ttccacctgc tgcgctctca tccagtttcg ctccgccgag
     6481 agcggcagcg gcatattcat tatcgtcatc gccatcgtca ttcgttgtca aaagcgttgc
     6541 acggcgcgct tcgtctttat caataaggcc tgcctcaaac agatctgcga tcgcgtcttg
     6601 cattgtaacc ataccgtagc gtgaaccggt ctgcatcatg gaatacattt gcggtatctg
     6661 gttttcacga atcaggttgc gcacagcatt tgttccaaca agtatctcaa atgcgccaac
     6721 gcgcccctgc ccatccgcct tacgcacaag agtttgtgct atcactccct gaagtgagga
     6781 agccaacatt gcgcgcacca tttccttgtc cccggtcgga aatacgtcaa tcacacggtc
     6841 aatggttttt gaagctgagt tggagtgcag ggtagcgaag accaagtgcc cggtttctgc
     6901 agccgtcagc gccaacgaaa tcgtttcata gtcgcgcatc tcaccaacga gtattacatc
     6961 agggtcttca cgcaacgcac ttttcaatgc acgcgcaaaa gacttggtgt cagtgccgac
     7021 ttctctgtgg tttaccaagc ttttttgtga ggtatgaaaa aattctaccg gatcttcaat
     7081 tgtcagaatg tgcttagaca tcgttaagtt gatatgattg accatcgagg ccagcgttgt
     7141 cgatttacca gaacccgtcg gccccgtgac caaaacaatg cctttttcca gttcggaaaa
     7201 acggcgcatt acgggcggca aaccaagctg ttccattgtt gggacttccg tcgggatggt
     7261 acggaataca gcggaagccc ccagtctgga tgtaaagcca ttgacacgaa aacgcgcttt
     7321 ttcgcccaga gcaatcgcaa agtcgatctc catattcttt tcatattctg cgcgctgatc
     7381 ctcagtcata actgaataaa gcatggtgcg aatatcgtca ctggaaagcg gatcagcttt
     7441 cacacgctta agcaaaccgc ttacgcggat aatgggcggg ctaccggcac ttaaatgaag
     7501 gtcggaggcg tcattttgca tcgtaaatgc aagaagctga gtgagatcca aggatactct
     7561 cctttttatt agaaacttga aggcaacgtg atttttatgc ctatagatag ttttaacgca
     7621 aatttataaa tggaaagtaa agatttttga ccttccgcta aagattttag cgctttttaa
     7681 agcgaatgag aacctcccat caacaaaagt ataaaaaagc tgacaatcag ggccgggccg
     7741 aatgggaata aagcttcatt tttaattttc tgccaagcca ctccgaataa aacaccaaaa
     7801 atccctccca gaatacaaaa aatgcctaaa tctcctaatc ccagccatag cccggcgact
     7861 gcaaaaaatt tgacatcccc cattccaagc gcttgttttt taaggatttt agtcattcca
     7921 gccgccaaca gccaggccaa tgtcatataa acaagggcac cacccaaatg ttccactatg
     7981 cggcctttca tagtgcctgg ctcaagataa acttgatacg cgaggcttgt taaccccagc
     8041 ataaaaatga ttgcgactaa aatattgggc agggttttat ggcgaatatc aatcatgagc
     8101 agcgccaaaa ggaacggtaa catcgccaag acgataaaac tctgcatcat atccttcgcc
     8161 gggcaaaaaa taaagatcga gatggcgagc agtgcggcgc ttagctccaa aaaaggatat
     8221 aaagcaggta tactggcaga acaataacgg caacgcccac gcgaaaacag ccaggaaaag
     8281 agcgggataa gatcacgtac tcccagagta tgattacaat acggacatgc agagcgctgg
     8341 ccagggctga acactccgac gccgcttggc aggcggtgga tcatggcact ggaaaaactg
     8401 cccatgatca gacctaataa tgcgaatact acaagcaaga ggacgccgaa atcacccaaa
     8461 accagtggat ttacgttcgg catatcaggc acgaatcagc gaagctgcgc taagcgctca
     8521 aagacggttt cacgaggaat gcttttactc gcgccgtaaa gcgtgtcgac ctccagtgcc
     8581 tcttcataat agcggacagc ttgcttttta tcgcctgcgc ggtcagctat tacagccata
     8641 ttaaatatat ggtttgcatt attcggctcc atgctcgccg ccataccaag atagcgaatc
     8701 gcatctggat atctccctaa ttgagcttca ataacagcta tctgggcaat gattgcggta
     8761 cttccagggt tatcctcatg cagcttttgt aagtttatga gtgcaaccgc cggataacgc
     8821 cgccccataa ggccttgcag attgatttgc gcttcgacat tgcttggacg aatctccaga
     8881 agctcttcat aagccgcaat cgcctcatca aaacggcccg cacgctgaaa agatgtggcg
     8941 cgccctaaaa gaatattcgg atcgcgcttg tttgttgcat agagcccatc atatatctca
     9001 agggccgatt catagcgccc cagttttgta gcgcgctcag ctgcaaccag gcgagcctct
     9061 ttgctgtcgg cgtcaaattt cttgcgcgta ataaccaaac gggaggccgg gtttagcgca
     9121 ggattaacct tagacggcgc accctgacgc gaaagatcgc tggtagggac gtatgcttcc
     9181 gcgtcaaaga acaaatcttc atcaaactca cccccgtctt cggtctgacc ttccggtaac
     9241 gcaggcactt ctgccgcctc tgtagctgac attaagtctt gctcttccag cggcgcggcg
     9301 ggcgatgtaa tttcttgtat atccgtttcc tgctttacat cagacgctgc gttcttttgt
     9361 atcagcagat cctgaattac cgcctcttcc tgtgcattca tttcaacaga aagctcctgc
     9421 gccctgtcca atacctgcct tgccgattgc gcatgggcta tgaacgggct cagcccgaca
     9481 cctgctataa agcagacacc tagactatag gttttgagct taaagtgtga aggaatttgg
     9541 aaacgccaca tcatgatata aacctgtcga tagaaatact gccgaagcag cagcgaggga
     9601 ataaagtgcg aatcagtaga acaatactcg cactttcaat gtatcacgct gcgctctttt
     9661 gctcaataaa atgtagaaag gcttgttcga gggacaaatc accggacaga gctttcagtt
     9721 tatccggttt tccggtaaag cgaatggaac gatcatgcat cacagcaacg cgatcacaga
     9781 tctcatccat atcagccaga atatgagagc tcaggaaaac ggtgcaaccc ttctgccttg
     9841 cttctacaat aagagttttt acgagtgctc tagcgacagg gtctaaaccg ctcattggct
     9901 catcaaggat tagcaaatcg caatccgtca gtatcgtggc cagcacccct aatttttgac
     9961 gcattccctt ggaataggta tgaacacgcc ggtcaagagc cgctgggtca agggctatgc
    10021 gctcggcata ttctatacac tgcgcattgg aaatgctggt tttgtacata cgcgcggaga
    10081 attttaaaaa ttcgaggccg gttaagaacc atggcggctc aaagcgctct ggcaaaaaag
    10141 ccaaacgctg ctttacggct ttcgtgcctg ctctttgatt aaagatcaaa atctccccgt
    10201 cactaggcgt acgcaaatcc aaaattgctt taatgagggt ggtttttccc gcgccgttca
    10261 gaccaatcaa accaaaacat tcgcttgcct ttatattaag atcgacatcc tggatagcct
    10321 taaacgctcc gtaggatacg ccgacatttt ttatacttag cgcgtcttcg ggtttttgct
    10381 gttggacttt ggctgtactc attgtttcac taaacctagc cgggcaagaa tatgcgcgta
    10441 tcaatattaa aacgctggtg tggtcgcaag cggattggga aaatctggtt ggtgtattcg
    10501 tcgaaccatt taaattctat atactcctta tagactctca gatccataac ctctttggga
    10561 atagcatccg gagttatgcg ctcttcattc agccaaattg cccagtctgc tttaccatga
    10621 aaaacaatac cagacaggcg aatttcacgt tcctcgggag gtggtttgat tttttcgcca
    10681 atatcctcac gctgtttcaa atctttcgca agctcactat ctgttacatt gcgcgtgtca
    10741 ccgacatatc ggcgagcgtc ctgaattgca acatgttccc aatgcgtaaa aaggatagaa
    10801 tcgtattgta agggatcaaa tcctttcttc gaatttgcat ctttgtcagc agacgtttga
    10861 ttttccgaaa gggtttcggt cgccgccgtc ggcgtttctc gctgcacaga ttgagcgcgc
    10921 ggcattaccg caaacccgaa agacatcaca acaataccta aaagaagaag gatatagtat
    10981 ctcattattt cccccctccg ttaatgacgc tcgcctctgg gatcattgta cgccacatca
    11041 actcgacctg cgcgcccaca agcgatgggt ttttgccgtt tgcaattgcc cgcagcaccg
    11101 ttccagttac atcggcttct ctttttaagc taattttttc gacagacaaa tgccctggga
    11161 agtaattttc agccaaaaac agataatgaa aaatattcac atcatcaaat gcctctaggc
    11221 gaatttcgac aggacttcta agaattttat gctcagcttt ttgcgcttcg ggattatctt
    11281 ctattgagcc ggattttacg ctggcaatag cttttgaaac ccctgagttt ttttgtatga
    11341 cgttgaacat tttttcagcc tggcggcgac tttggtcttt gaagaatccg cgccttacca
    11401 gtttctcgaa ttcttctttc tgatcttcca gttgttcaaa ttcaacctgc atccgattaa
    11461 tgtccgtgcg caacgtagaa acagtgccac gcaaccctct taattcgcgc tccttaactt
    11521 gtatttcagg gatgaaatat aaatacaaac cagcagccag aaatgcgttg agcaacaaca
    11581 aagcagccaa cacaaaaata cgttttatac ccaaaactgc gatcattctt tcgcgggccc
    11641 ccttataaga atttctgcca caaagtcttg ttgaatatcc tgtttttcaa gatctcccgc
    11701 ctcaacaata agaccttcgg tatattcata atccttgagg aatttagtca cctcaacttt
    11761 gtaacccggc agcagacttt caatacgtac gctcaaatcg gcaacctctt tattcccctt
    11821 atcgacattt gccgtcgatg gatatgtcat tttcatgcgc gtttcaaatc ttggttgcga
    11881 ctcattttta actgcgctcc acacattctg cgcaatcgta ggctttactt tctttatctc
    11941 aatactatca atacgcaagt ccttacccag agccttgccg accttctgaa agacggtaag
    12001 aatcggaatg cggtcatctt caaactcctt atgaacagca agcgctcctt gaacaaggcg
    12061 agcatcgaag ccaaccttat caagacgctg aacctcacgc tgatattgtg catcgagttg
    12121 tgatttattg cgcttcaaac cttcaatatc agacaccaga gatgccagcg atccagcact
    12181 tgatacaacc tgatagccca ggaacgcaga tccacacaac agcgccattc cgacaagcat
    12241 cgctacttga cgcggttttg agacttcatc aacctgcgtt gccttcatcg gcaacacgaa
    12301 acttcccttc cgaccagccc aagccacatg cagaacatcg gcataacgca tatcggcttg
    12361 acggcctata ttcaccccta acgctctggc ggcgtcatcc acgcccaaaa tatgaagatc
    12421 atagtcttca tcaaatatag agcgcaaagc ttcagccggc gcaggatttg ctatgacgaa
    12481 cacatccaat ccatcagcgg gatcataacc aaaacgtgaa agatagctga tggttgattt
    12541 aaattcctga tacaattcag atgcccatac ttgaggatca tcatccttct cagcaatagg
    12601 actcatccgt gtaagcgcta gctcaccgtt ttttgtcaga acctgacgca ggcttccatt
    12661 ctgatgctga ccaataaaaa tggaccatac attcttttta cgtccctttt tggccccgat
    12721 cttacctgac aaggctttga ccatatcgga ggattcaatc ggcagcagac aaaatgccgc
    12781 aacagaggcc attgattttt tagttgattc aatcgttttg gtaatctgat cgtttccagc
    12841 aacggctgca aatatataaa tatcagcagc aggctgagag tcagtcttcg ccaccttttc
    12901 ctttaaagga aacgccgcgc gaattggata atgaggaaac gctacattaa gtttgcgctt
    12961 aaccattgac gctttatcaa ccatccctac accggtgcgc accacccgct ccttgcggta
    13021 atgctgctct accatatcat tgagaatcaa aaccggtttt ttaccacaat ctttggcaat
    13081 aatgctggcg acgtttttct caaaattttc cgcttcccat ggaatcgtat cgataagctt
    13141 tacgccaggt cctctaactg tatagataga caaaaattcg tcggaaacga gcaatactgt
    13201 acgattagga gcaagaaaag aaagtgacat cggtacttaa aaatccataa agtttccaag
    13261 gttcatgtag attggtccca ggctgcctgc agcaatccaa gcaatgatga gtccgagtat
    13321 agcagttaat gtcggctgaa tcatctcaat taacgcttca atcgcttcat ttacatcatg
    13381 tgtataaaaa tcagagacct gatcaagcac cggtgttaga ttcccggact cttctccgac
    13441 cttaaccata cgtacaacca gacttggaaa ttcaccactt gcattaaatg cttcggacaa
    13501 tgcacttcct gccttcacgt gaccttcaac gctttccatg gcatccagca tcatcaggtt
    13561 tgtcacggtc gctcgcgagt tttccagcgc gctgattaca ttgatcccgc ttgcataaag
    13621 cgcaccaaat gtttgagcat agcgcgcaat agaaattttg cgaataagcg ggccaaccac
    13681 gggggcattc agccaccacg aatcaagctt ataggctata gcgtctgagc tgtggcgcaa
    13741 aagctttacc gttccaaaaa taatcaccgg aacaaccatg acaataatcg agccatatac
    13801 aggcacccca aagaagtgaa actggggctc aaccataaag tcagatgtcg caattaatgc
    13861 aatcgtgaac catggcagat caagatctag gaaccttaaa aagcctacaa tctgcggcac
    13921 gactatactc atcataaaca caacggtcaa aataaccaca acgctgacga tgatgggata
    13981 gcgcgtagcc tttctgattt tggcctgaat gacatcaacc catttcaaat attttatgag
    14041 ttggaggtat gaggctgtta aatcccctgt ttcttctccg gcttcgacca aggagatata
    14101 aagcggctta aagactttgg gatgcttatt catcgcctca gacaacgccg cgccgtcaga
    14161 tacatcgcga aaaatttctg acattatatc acgcagacca tcatgctcgg ttgaatcgcg
    14221 cacatcagaa agcgcatcaa gcaacggtac gcctgcgctt tgcatctgct ccaattgtac
    14281 aaaaagctga ataacatcgc gaagcttgat tttcttttga ccaatctggc caaaaccgcc
    14341 cttagacttg gttgactgtg cagagcaatc caccaactca agcccggcag actggagttg
    14401 cttgtaaagg tcaacctcac tattggccgc aatgttaccg cgaatgggcc gacctttaga
    14461 gtttattgct ttgtacttat agcgtggtat gattcaggat cctttacatg aagtgaaaat
    14521 caccgtctta tatcaacgac tttggcgagc gcctccaggg atgtaacccc ttccagaact
    14581 tttagaatgc catcgtcttt catgcttttg aagccttttt tcacggccat ggcttttaac
    14641 tcagccttat ttgcaccgcg cgcgagcaat tcatccattt cttcatcgaa tgtcaggatt
    14701 tcaacaacgg ctgtccgccc tttatacccg ctgccgctac agctatcgca tccgccctgc
    14761 cttgcgcgat aaattgttgg catatttgct ggatcagcac ccaaaatctc acattcttgt
    14821 gcatcaggct ggtacgcttc tttacagacc ggacacaatc tgcgcgtcag gcgctgtgca
    14881 aaaaccgcaa cgatagctcc ggcaaccatg ccaggcttca accccagatc gaacagacga
    14941 ggaattgcgc cgaatgaatc gtttgtgtgc agcgttgtat aaacctggtg ccctgtcatc
    15001 gcagccttta acgccatgcc agccgtttca ccatcacgaa tttcaccgat aaagatgatg
    15061 tccgggtcct gacgcaaaag cgctttgatg ccatctgcga attccagtac gccttcacgc
    15121 acgtgcgtct ggcgaatcat cggcaaagaa ttttccaccg ggtcttcaag agtctggata
    15181 ttcacctcga catcgttaat ttcattcaac atggaataaa gcgatgtcgt tttcccagag
    15241 ccggttggac ccgttacgat aataatacct tcgggcttac tttgtgcctg acgaatcaga
    15301 ttgcggttat gattgctaaa gccgagtttt tccatcggca taatgctttc actctgatcc
    15361 agaacacgca gtacgatgtt ttcaccgtgc accgtcggca gagaagacac gcggaaatcg
    15421 gcctcgcgcc ccccgacatg cataccgaaa cgcccgtctt gcgcacctaa tttatccgct
    15481 atattcatgc cggacataat cttgatgcgt tgagaaattc cgctccaatg ctgtttatgc
    15541 agaatttgcg ctgtaaaaag cacaccatcc ttgcgataac gcagacgaat aaagttttct
    15601 tccggttcga agtgcaagtc agatgcacca atctttacag cttcaaaaac caacgcattt
    15661 acaagacgta caatcgggtg tgaaaatgct tcgctttcat ctagggacgc tacatctttt
    15721 tgctctttcc cctcttcaag ctcttgcaag atcgcgttga ccgagctggc atacccataa
    15781 gctgcatcaa tcgcctcact caggattttc ggcgtacaca ccatcggctt gactgtcagc
    15841 cccttgggaa tgaagcggcg aagcatatcc atagcaacaa tgtcgtaggg gtccgccata
    15901 gctaccttga tgtggtgctc gctcatagat accggcaata ccttatgctt ggcagcttct
    15961 tccttggtaa tcagccctaa gacctcgcca tcaaaaattg tattttgcgg atcaaagagt
    16021 tcaacgccag aactttgagc aataaaagcg ctcaacgtct cttcatcaat aaaccccagg
    16081 tcaaccatga cctcaccgag catcttgcca gtaattttct tttcctgaag agccacgttc
    16141 agctgatctt tggaaatcaa tcccatctca accatacgat cgccgatgcg cccttttctg
    16201 cgtagatcat cgtcctcttc agattcattt atagtaatat tacgactgta cgtcttgaga
    16261 gcatggcccg tatcttgtct ctgctgttgt gattgtgatt gtgattgctg cgtttgattt
    16321 tcacgcgcag gcacaaaacg cgcaggaagc tgcgcagcgc ttttttcatt ttcagaatta
    16381 ggcagatttt gatcaatgac ttcaacctga acaggctctg catcttcttc taatacgagt
    16441 ccatgaaaag tctctgatat cggcgcctct gttgccttta cagacttaga ttccttaaca
    16501 atgataccct caatgggttc tttatcgtcc ctatggatca tttttatgct ctctacccta
    16561 agaaaatagc ttttgagtat tatcagaaga gttacgccat tcgagtaatc aggatatcct
    16621 agaaaagtta acaaaacaaa tatgtttatt tacataactt ttaagattta aagaaaaaag
    16681 aaggcaccga tctaaaattt ggtgccttct ttaatttaat accatttaaa actgtgttta
    16741 ttcagcagca acagctgtaa ttttcttatc tcgacgtggc caggagctaa atctcatcac
    16801 agattcatcc acgccgcata ctgtcgtaca taaacgcgca gcaaccaaat acgggtctgc
    16861 atttgcaccg ggacgacgat cttcaaaata gccataccct ttgattgcaa caggttttgg
    16921 aatacggatc gaacagccgc gatcagcatc tcctgccttg aacgtattga tatcgcatgt
    16981 ttcatgcagc cctgtcaggc gctcactcag gcctgcgcca taaagtacga tatgctctgc
    17041 gtgttttttg ctcagtgcgt ctacagcagc atcgattgcc gcacgacctt tagatttatc
    17101 gcgggtatcg cttgtagaga agtttgtatg catgcctgca ccattccagt cgcctttgac
    17161 cggtttattt tcaagcgttg ccgcaatatc aaactcttcg ccaacacgat ggagcagcca
    17221 gcgcgcaacc caaacctgat cagccatatt taaaacgcct gaatcttcag aattatcacc
    17281 acgatagcca atctgaaatt cccattgacc gggcataact tcggcgttca tgccgtaata
    17341 cattagacca gcatcaaggc agagttcagc gtgagtttct gctacctcac ggccaaaggc
    17401 ctcttcggcg ccaacgccgc agtaatacgg cccttgtggc gccggatagc catgtgacgg
    17461 ccaaccaagc ggcgtacgat gaccttgtgg gaagagcgta tattcctgct caaagcctgc
    17521 ccagggatcc tgcttactgg cgccggcgtc cagtacttcg cgcagttgcg cgcgtgagtt
    17581 ggattcgtgc gggcttccat cagggttatt cacctcacac atcacgagga agttgccttc
    17641 accacggata gggtctttga aatatgaaac aggttgcaag atgcagtctg aatcattgcc
    17701 tgcggcctgt tgcgtagatg atccgtcaaa gctccactct gggaactccg taatttttgg
    17761 cttggcacca aggtttacaa cacgcgtttt cgaacggaca aaacgcgtgg ggacacttcc
    17821 gtcaagccag atatattctg ctaaaccaaa attagtggac atgctattta actccttgct
    17881 atcagcattg ataaaa
//
LOCUS       DAOSWR010000028        17773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2016_length_17773_cov_5.460831, whole genome shotgun sequence.
ACCESSION   DAOSWR010000028 DAOSWR010000000
VERSION     DAOSWR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_17773_cov_5.460831"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /gene="rpsC"
                     /locus_tag="PK513_03480"
     CDS             <1..290
                     /gene="rpsC"
                     /locus_tag="PK513_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO81546.1"
                     /translation="MKRAVQSAMRLGAGGIRINVSGRLGGADIARTEWYREGRVPLHT
                     LRADLDYGTAEALTTYGIIGVKIWLYKGDIMEHDPMGRDKRLEAQGGAPRN"
     gene            478..906
                     /gene="rplP"
                     /locus_tag="PK513_03485"
     CDS             478..906
                     /gene="rplP"
                     /locus_tag="PK513_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOO81547.1"
                     /translation="MLSPKKFKHRKQHKGRIHGDAKGGYSLAFGAYGLKALSPDRITA
                     RQIEAARRAITRHMKRQGKVWIRIFPDVPVTAKPLEVRQGKGKGSPEYWAARVKPGRI
                     MFELDGVTQDVAREALALAAMKLPVKTKFITRVGVNQEAA"
     gene            908..1162
                     /gene="rpmC"
                     /locus_tag="PK513_03490"
     CDS             908..1162
                     /gene="rpmC"
                     /locus_tag="PK513_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOO81548.1"
                     /translation="MKAEDLKTKTPDELKKLLLENKKAQFNQRFQKANGAFENTSEIR
                     KTRRTIARIKTFMNAKPEEKAAAQKASAKKKAKASKEKAA"
     gene            1177..1401
                     /gene="rpsQ"
                     /locus_tag="PK513_03495"
                     /pseudo
     CDS             1177..1401
                     /gene="rpsQ"
                     /locus_tag="PK513_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            1572..1940
                     /gene="rplN"
                     /locus_tag="PK513_03500"
     CDS             1572..1940
                     /gene="rplN"
                     /locus_tag="PK513_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOO81549.1"
                     /translation="MIQMQSRMEVADNSGARQVQCIKVLGGSKRRTASVGDIVIVSVK
                     EAIPRGRVQKGNVQRAVIVRTKKGLNRAYGETISFDTNACVLINNNNEPVGTRIFGPV
                     TRELRAKGFMKIISLAGEVL"
     gene            1942..2265
                     /gene="rplX"
                     /locus_tag="PK513_03505"
     CDS             1942..2265
                     /gene="rplX"
                     /locus_tag="PK513_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO81550.1"
                     /translation="MASAKLKIKKGDSVIVLTGKDKGAKGEVLKVMPTERRVIVQGVN
                     VVKKHAKPTQFSAGGIQSKELSIHVSNVALADPKSGKATRVGYKTLKDGKKVRVAKKS
                     GETVE"
     gene            2271..2828
                     /gene="rplE"
                     /locus_tag="PK513_03510"
     CDS             2271..2828
                     /gene="rplE"
                     /locus_tag="PK513_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO81551.1"
                     /translation="MAKAAYKPRLQEFYETEVVPAMQKKFEYKNKHEIPQLVKVVMNM
                     GVGEATQDRKHIENAANDMSLIAGQKPVITKAKQSNASFKIRDGQAIGCKVTLRRQKM
                     FEFLDRFVTIALPRVRDFRGISDKSFDGRGNYAVGIKEHIIFPEIDYDKVDKIRGMDI
                     VICTTAKTDEEARELLRLFKLPFKK"
     gene            2847..3152
                     /gene="rpsN"
                     /locus_tag="PK513_03515"
     CDS             2847..3152
                     /gene="rpsN"
                     /locus_tag="PK513_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOO81552.1"
                     /translation="MAKVCMIERDKKRSRLVKKYAGQRAKLKAIIKDQNAPADERFQA
                     TLALAELPRNSAKIRQRNRCALTGRPRGYYRKLNLSRIALRDLASRGELPGITKSSW"
     gene            3168..3566
                     /gene="rpsH"
                     /locus_tag="PK513_03520"
     CDS             3168..3566
                     /gene="rpsH"
                     /locus_tag="PK513_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011909476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO81553.1"
                     /translation="MSMSDPLGDMLARIRNGQMASKSVIECPHSKLRENVCKVLADEG
                     FIRAFKKEERENNKAVLVIELKYAEGRGVIRQITRVSKPGRRIYKGAKDMPRYYNGLG
                     VLIVSTPKGLMTDHGARAANVGGEVLCQVF"
     gene            3585..4163
                     /gene="rplF"
                     /locus_tag="PK513_03525"
     CDS             3585..4163
                     /gene="rplF"
                     /locus_tag="PK513_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOO81554.1"
                     /translation="MSRIGKQPITLPEGVELSVNGQDVKAKGKLGQLELRVHDEVSIK
                     IEDVANDDGKTDKVVTLAPKSNSRFARQIWPTMRTLVNNIVVGVSEGYTKKLEIKGVG
                     YRGNLQGKTLVLSLGFSHEVRYDIPEYVTVKMEDAAGKGSQTDIQISGIDKQQVGQVA
                     AKIRGFKPPEPYKGKGIRYADEYVIRKEGKKK"
     gene            4188..4574
                     /gene="rplR"
                     /locus_tag="PK513_03530"
     CDS             4188..4574
                     /gene="rplR"
                     /locus_tag="PK513_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOO81555.1"
                     /translation="MSKALTSAQRRTYRTRNKIRRVNIDRVGVRETRPRLSIFRSGKQ
                     IYAQVIDDIKGVTLAAASTLELEMKEKLKKGGANTQAAAEVGKLVARRAKKAGVEKVV
                     FDRGRFLYHGRVKALADGAREGGLLF"
     gene            4605..5156
                     /gene="rpsE"
                     /locus_tag="PK513_03535"
     CDS             4605..5156
                     /gene="rpsE"
                     /locus_tag="PK513_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOO81556.1"
                     /translation="MARGQGQEAEREEPEFTERLVGINRVAKTVKGGRRMGFAALMVV
                     GDGKGRVGHGHAKAREVPEAIKKATEQAKRNMIRVPLREGRTLHHDVKGRHGAGKVFM
                     RSAPQGTGIIAGGPMRAVFEAVGIQDVVAKAIGSNNPYSMVNATFAGLKAMQSPRHVA
                     SRRNKKVGDVVSGRGDKEAHGEE"
     gene            5430..5603
                     /gene="rpmD"
                     /locus_tag="PK513_03540"
     CDS             5430..5603
                     /gene="rpmD"
                     /locus_tag="PK513_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOO81557.1"
                     /translation="MTVTQIGSPIGRKAYQRATLVGLGLNKMHRTRTLEDTPSVRGMI
                     NKVKHLVKVEDAA"
     gene            5626..6123
                     /gene="rplO"
                     /locus_tag="PK513_03545"
     CDS             5626..6123
                     /gene="rplO"
                     /locus_tag="PK513_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOO81558.1"
                     /translation="MKLNELKNNDGAKKARIRVGRGIGSGKGKTSGSGQKGQKSRSGV
                     AINGYEGGQMPLHMRMPKSGFNNKKFCVHYVELTTGKLQAAIDAKKIDSKKTIDEDTL
                     VAAKIISRKKAGVKLLTKGELKAKVELKISKATKTARAAVEKAGGKIEIIEKTVAPVE
                     NKKKK"
     gene            6309..6998
                     /locus_tag="PK513_03550"
     CDS             6309..6998
                     /locus_tag="PK513_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81559.1"
                     /translation="MSILVYAAQASAHSSSKSHNHKSSQSAPEQLSCKASKPSLSVKT
                     ATTRTKYIRTKSSKDLTQLHGGGGSSVGGLGGGEIGFKTEGKFEITEAGGKACVKLKQ
                     LEVTFYAKPEIHIASNFNRSTCEHNAVMAHEKGHIRILRKFVREFSPKVKVFLSKYVY
                     KIDPAIGPIPKSQVNEAQKKIQEQYMSKLEAYQNKIMPILGKRQKAHDSPQEYARVAS
                     KCSKWDKKLAQ"
     gene            7127..8488
                     /gene="secY"
                     /locus_tag="PK513_03555"
     CDS             7127..8488
                     /gene="secY"
                     /locus_tag="PK513_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO81560.1"
                     /translation="MASAVEQLAKNANWGALAKATDLKRRLLFVMGALLVYRLGTYIP
                     VPGIDPHVWNEIFTQKGGGILDMFNMFSGGALSRMTIFALNIMPYISASIIMQLATAM
                     SPQLEAMKKEGGTGRMKINQYTRYLTVLLATVQAYGLAVGLESMQGSTGSAVIDPGLF
                     FRISTVITIVGGTVFLMWLGEQITARGIGNGISLIIFAGIVAELPRAIASTLELGRQG
                     TFSGLELLALFLMIAGVIVFIVFMERAQRRVIVQYPKRQQGNQMMGGETNHIPLKLNT
                     AGVIPPIFASSLLLLPLTIVGFSGAEGGDFTATISQYLAHGQPIYMALYGALIMFFCF
                     FYTAIVFNPEENADTLRKYGGFIPGIRPGKHTADHLDYILTRITVLGAAYLVFICLLP
                     EFLISQYNMPFYFGGTSLLIVVSVTMDTVAQIHSHLIAHQYEGLMKKAKLGKRRGMMR
                     GTR"
     gene            8510..9076
                     /locus_tag="PK513_03560"
     CDS             8510..9076
                     /locus_tag="PK513_03560"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOO81561.1"
                     /translation="MNLIMFGPPGAGKGTQAKLIQDKYRIKQLSTGDMLRGEVASGSN
                     MGKRLKSIMDSGELVSDEIIIEIIANCISEPECEKGFILDGFPRTVDQAEVLDNMLSH
                     MGRKLDHVIVLEVDEEALIDRIQTRAAETGGQRSDDNAETLKNRLKVYNDQTAPVLPY
                     YKNHGILRHIDGMQSITDVTAQIDAVLK"
     gene            9255..9626
                     /gene="rpsM"
                     /locus_tag="PK513_03565"
     CDS             9255..9626
                     /gene="rpsM"
                     /locus_tag="PK513_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO81562.1"
                     /translation="MARIAGVNVPDKKRTPIALTYIHGIGRTTAFKICDKLGIDVQKR
                     MGELDEDTLNRIRAEIDTDAYMIEGDLRRETSMNIKRLLDMKCYRGLRHRNGLSVRGQ
                     RTKTNCRTRKGPAKPIAGKKK"
     gene            9641..10030
                     /gene="rpsK"
                     /locus_tag="PK513_03570"
     CDS             9641..10030
                     /gene="rpsK"
                     /locus_tag="PK513_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014875982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO81563.1"
                     /translation="MAKPKTKAKRKEKKNIVSGDVIVNSTFNNTLISFTDKQGNVVSW
                     SSAGMMGFKGSRKSTPYAAQMAAEDAGRKAQEHGMKEVDVRVKGPGSGRESALRALQN
                     IGFTIKQINDVTPIPHNGCRPPKRRRV"
     gene            10148..11164
                     /locus_tag="PK513_03575"
     CDS             10148..11164
                     /locus_tag="PK513_03575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOO81564.1"
                     /translation="MIQKNWQELIKPSKLDISHSEVPNTGKVVAEPLERGFGLTLGNA
                     LRRILLSSLQGAAVTAVQIDGVLHEFSSIEGVREDVTDIILNIKALAVRMHAEGPKKM
                     RLSAEGPCEVTAAQIEAGADIEIMNPDLVICTLDKGAKLSMEMTVDTGKGYRPAAQNR
                     PEEAPVGLIPVDSVFSPIRKVTYEVEDTRVGQITDYDKLTLTLQTNGVITPEDAVAFA
                     ARILQDQLQVFVNFDEPEVATEKEEEHELPFNKNLLKKVEELELSVRSANCLKNDNIV
                     YIGDLVQKSESDMLRTPNFGRKSLNEIKEVLTIMGLHLGMQVEGWPPENIEDLAKKAD
                     DQQF"
     gene            11354..11767
                     /gene="rplQ"
                     /locus_tag="PK513_03580"
                     /pseudo
     CDS             11354..11767
                     /gene="rplQ"
                     /locus_tag="PK513_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            12228..12455
                     /locus_tag="PK513_03585"
     CDS             12228..12455
                     /locus_tag="PK513_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81565.1"
                     /translation="MSASLIPMNDPSQVKGPDGKVDQNSIYLFQRGITNATVDARRQG
                     DERFWSCYLTLSLAQEPFFSPGLDKPEHDLH"
     gene            complement(12510..13931)
                     /locus_tag="PK513_03590"
     CDS             complement(12510..13931)
                     /locus_tag="PK513_03590"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOO81566.1"
                     /translation="MLTKVFVLLLALCAVNVNPAAAQTKLLPQSQQQIQLSFAPLVKQ
                     TAPAVVNIYTKRTVSQRSVHPFARDPFFAPFFRDDFFGGRMRKQIESALGSGVILASD
                     GLVVTNAHVIRDADEITVVLSDGREFEARLALADEPSDLALLRIDDLRGESLPYVSLK
                     PSETLEVGDIVLAIGNPFGVGQTVTSGIVSAQGRSSLDINDFNFFIQTDAAINPGNSG
                     GALVALDGGVVGINSAIYSRDGGSLGIGFAIPSEMVASVIAAEKAGQSGARGVIRPWL
                     GVTSQNLSADIAASLGLKTPHGTLINALHPASPLKKAGVKVGDVILSLNDKEIRDASE
                     MKFRMATVPLGDKAKVTLMRQDQKLNVYVEAMAPPEDPPRDETSLSGEHALNGAVVGN
                     VNPAVALELGLSSDAQGIVVLKVARASRAARVLSPGDLLVEINGRKINDVKDIEKALN
                     RGSGQGLSLVIQSGGRLQRIVIR"
     gene            13976..14803
                     /gene="panB"
                     /locus_tag="PK513_03595"
     CDS             13976..14803
                     /gene="panB"
                     /locus_tag="PK513_03595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOO81567.1"
                     /translation="MRKTIEEIKSHKNQPEPLVCLTAYTARIAQILDPHCDLLLVGDS
                     MGMVLYGMDSTQGVSLEMMINHGRAVTCGSTRSCVIVDMPFGSYEESPAQALNNARRV
                     MDETGAQGVKLEGGVEMAATIKTLVKAGIPVIGHIGLMPQSVTKETGFRVKGRSADSE
                     AQLLADAKAIEAAGVSAFVIEATIESVAAEITLAAGVPTIGIGASAVCDGQILVSEDM
                     VGITSGHVPKFVKQYAQVGEIIGDAAARYAADVRARAFPAEAQLYGMSAAGKVKKAS"
     gene            14800..15594
                     /gene="panC"
                     /locus_tag="PK513_03600"
     CDS             14800..15594
                     /gene="panC"
                     /locus_tag="PK513_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOO81568.1"
                     /translation="MSLSVHHTQKDLQVLVQDWKAQGLRIAFVPTMGALHEGHLSLMR
                     LARGKADKVVVSIFVNPTQFAPHEDFDTYPRDLESDVELLKSAGVDAVYAPTEGDLYP
                     EGAVSDQKAGAAAAGLESDFRPHFFDGVVSVVYRLFKAVEPDVAVFGEKDFQQLQVIH
                     EMVVDKDMGIEILGGPIARDKFGLALSSRNAYLGAGELEIARMLNQVVYKAAENPSNE
                     ALEEAAAMLVEAGFDKVDYVAYRPEWKRVLTAAWLGKTRLIDNCGV"
     gene            complement(15736..16041)
                     /locus_tag="PK513_03605"
     CDS             complement(15736..16041)
                     /locus_tag="PK513_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81569.1"
                     /translation="MSSIMQHTHAPSSVAGYKEVTPDMRDTVKKEITSGNTSDMTGEH
                     VHTGDPMIEAFEDAEWVSAFDFDVGVFNMAASNNSLFWSYEEEEEEGEEESLEPEAA"
     gene            16448..>17773
                     /locus_tag="PK513_03610"
     CDS             16448..>17773
                     /locus_tag="PK513_03610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOO81570.1"
                     /translation="MPVDMKDIVALCKRRGFIFPASDIYGGLNGFWDFGPLGVELKNN
                     LRDRWWKAMVITPPLGPDGAPVDIVGIDSAIIQNPKTWVASGHVGGFSDPMVDCRETK
                     KRYRQDHLIVLTSEKADRWPAFHEETEDSEMEKKLKKLGMPSDLSLFEKINLTKLPIE
                     DYAKIIGPDTKEPGTLTEPKAFNLMFETHAGPIQSEENKVYLRPETAQGIFLNYKNVL
                     DSTRVKMPFGIAQIGKAYRNEVNPRNFIFRSREFEQMEMEWFCHPDEAKKWQEFWFDE
                     RVKFWKSVGLTEGNMEMRKHDADELAHYAKEGLGTSDIEYKFPFTDPGFGELEGIAHR
                     CDFDLKSHQEHSGVKMEYLDPARDNERYIPHVIEPAAGLTRGVLALICEAYTVDENRP
                     SGVYLNFTPAMAPKKAAILPLTAKEEHVPIAQKLYLELREEFPVDLDIKQ"
BASE COUNT         5005 a         4277 c         4563 g         3928 t
ORIGIN      
        1 ccatgaagcg cgctgtccaa agcgccatgc gtttgggcgc aggcggtatc cgcattaacg
       61 tatccggtcg cctcggcggc gctgacattg cccggaccga atggtatcgc gaaggccgtg
      121 tgccgctgca cacactgcgc gctgatctgg attatggaac agccgaggcg ctgacaacct
      181 atggaattat cggcgtaaaa atctggctct ataaaggcga tatcatggaa cacgatccaa
      241 tgggccgcga caagcgtctt gaagctcagg gcggtgcgcc gagaaactaa ctttgttttg
      301 gcaagttttt tcttgcaact tgatggtagg caggatataa agcgccagaa tctaattgaa
      361 cgctgcgaaa acgccgaaag ataaagcgtc catatgggaa ctctcttacg aagtaagaaa
      421 agtccatgtg attgatcttt ttgcgtgttt tgcctaagaa aataaggaac cagagcgatg
      481 ctctcaccta aaaagtttaa acaccgcaag cagcacaaag gccgcattca tggagatgct
      541 aaaggtggat attcattggc attcggcgcc tacggtttga aggccctgtc acccgatcgc
      601 ataacggccc gccagatcga agcagcgcgc cgtgcaatta cccgtcatat gaaacgtcag
      661 ggaaaagtat ggattcgtat cttccccgat gtgcccgtaa cagcaaaacc tctcgaggtt
      721 cgtcagggta aaggtaaagg ctctcctgaa tactgggctg cgcgtgttaa gcctgggcgc
      781 attatgtttg aacttgacgg tgtgacgcaa gatgttgcgc gtgaggctct ggctttggcc
      841 gcaatgaagc tgcctgtgaa aacaaaattc atcacccgcg tgggtgtaaa ccaggaggcc
      901 gcataagatg aaagctgaag acctgaaaac aaaaacgcca gatgagctga aaaagctctt
      961 gctggaaaat aagaaagcgc agttcaacca gcgctttcaa aaagccaacg gcgcttttga
     1021 aaacacatcc gagatccgta agacccgccg cacaattgcg cggatcaaaa catttatgaa
     1081 cgcaaagccc gaagaaaagg ccgccgccca aaaggcatcc gccaaaaaga aggcaaaagc
     1141 gtctaaagaa aaagccgcat aaaggaaacg aagaaaatgc caaaacgtat tctggaaggt
     1201 gaagtagtaa gcgacaaaat ggacaagacc attacggttt tggtcgaacg ccgtttcatg
     1261 cacccgctct acaaaaaata cattaaaaaa accgacaaat atgccgcgca tgatgaaagc
     1321 aacgctgtaa aagttggcga tcgtgttcag atcatcgaat gtgccccgat ttccaagcgc
     1381 aagacctgga cacttttcac cggcgatgcc gctgctgcgc cgaagaaaga agaaaagaag
     1441 cctgcttcta aaaaagccga tcctaagaag gaagagacta aaaaagcaga aaagaagccc
     1501 gccgctaaga agccagctgc taaaaagaca accgcaaaga agaagggccc aaaagaggga
     1561 gataagtagt catgattcag atgcaatcca gaatggaagt agccgataac tctggtgccc
     1621 gccaggtgca gtgcattaaa gtcctcggtg gttccaagcg ccgcacggca agtgttggcg
     1681 acattgtgat cgtttccgtt aaggaagcca ttcctcgtgg acgtgtgcaa aaaggtaatg
     1741 tgcagcgcgc tgtcattgtg cgtaccaaaa aaggtcttaa ccgcgcctat ggcgaaacaa
     1801 tcagtttcga caccaatgcc tgcgtgctca tcaataataa caatgagccg gtcggtacac
     1861 ggatttttgg ccccgtaacg cgtgaactgc gcgccaaggg cttcatgaaa attatctctc
     1921 tggctggcga ggtgctttaa aatggcaagt gcgaaactca aaatcaaaaa aggcgattct
     1981 gtcattgtcc tcaccggcaa agacaaaggc gccaaaggtg aagttctgaa ggtcatgccg
     2041 acagagcgcc gcgtgattgt tcagggcgtc aatgttgtca aaaaacatgc aaagcccacg
     2101 cagttttctg ctggcggcat tcagtcgaaa gaactgtcta ttcacgtatc aaacgtggcc
     2161 cttgccgatc caaaatccgg taaagccacc cgcgttggtt acaaaacatt gaaagatggc
     2221 aaaaaagtcc gcgtcgcgaa aaaatccggc gagactgtgg agtaggaata atggcaaaag
     2281 cagcatataa acctcgtttg caagaatttt atgagaccga agtggtccct gcgatgcaga
     2341 aaaaattcga gtacaaaaac aaacacgaaa ttcctcagct tgtaaaagtt gtcatgaata
     2401 tgggtgtcgg tgaagcgacc caggaccgca agcacatcga aaatgcggct aatgacatga
     2461 gcctgatcgc cggtcaaaag cctgtaatca cgaaagccaa gcaatccaat gcatctttca
     2521 aaattcgcga cggtcaggca atcggctgta aagtcacttt gcgccgtcag aagatgtttg
     2581 aattccttga ccgttttgtc accatcgcgt tgccacgcgt ccgtgacttc cgcgggatca
     2641 gcgataaaag ctttgacgga cgtggcaact acgccgttgg aattaaagaa cacatcatct
     2701 ttcccgagat cgactacgac aaggtcgata aaatccgcgg gatggatatt gtaatttgta
     2761 cgacagccaa aacagacgaa gaagctcgcg agttgctgcg tttgtttaaa ctcccattca
     2821 agaaataaga aaaggacgac gttgaaatgg caaaagtttg catgatagag cgcgataaaa
     2881 agcgtagccg tttggtcaag aaatacgcag gccaacgcgc caagcttaag gcaatcatca
     2941 aagatcagaa tgctcctgca gatgaacgtt ttcaggcaac tctggctttg gcggaattgc
     3001 cgcgcaactc tgcgaagatc cgtcagcgta accgctgtgc cctgactggt cgtccgcgtg
     3061 gttactaccg caagttaaat ctttcccgta ttgcattacg ggacctggcc tctcgtggtg
     3121 agctgccagg cataacgaaa tccagttggt aagaggagaa aagacctatg tccatgagcg
     3181 atcctctcgg tgatatgctg gcccgcatcc gtaacgggca aatggcaagc aaatccgtaa
     3241 ttgaatgccc gcattcaaaa ctgcgcgaaa atgtttgtaa ggtactggcg gatgaaggct
     3301 ttattcgtgc gttcaagaaa gaagagcgtg agaacaacaa ggccgttttg gtcattgaac
     3361 tgaaatatgc agaagggcgc ggcgtcattc gccagatcac tcgcgtgtct aagcctggcc
     3421 gccgtattta caaaggtgca aaagatatgc cgcgttacta caacggtctg ggtgttttga
     3481 ttgtttcaac ccccaaaggg cttatgacag accacggtgc ccgcgcagcc aatgtcggcg
     3541 gcgaagtgct ctgtcaggta ttttaggaat aaggacgaaa agcaatgtca cgtattggaa
     3601 aacaaccgat tacactgcct gaaggcgttg aactcagcgt caacggtcag gatgtgaaag
     3661 ccaagggtaa acttggccag ctggaactgc gtgtacacga cgaagtgtcc atcaagatcg
     3721 aagatgtcgc caatgatgat ggtaagactg acaaagttgt gaccttggca cccaagtcaa
     3781 acagccgttt tgcacggcag atttggccga ccatgcgcac actggtcaac aatatcgttg
     3841 tcggagtcag tgaaggctac accaaaaagc tggaaatcaa aggcgttggt taccgtggaa
     3901 atctgcaagg caagactctg gttctgtctt tggggttctc acacgaagtg cgttatgata
     3961 ttcctgaata tgtgaccgta aaaatggaag acgcagctgg caaaggcagc cagaccgaca
     4021 ttcaaattag cgggatcgat aaacaacagg tcggtcaggt tgcagccaaa atccgtggtt
     4081 ttaaacctcc tgagccatac aaaggcaaag gaattcgcta tgcggatgaa tatgtcatcc
     4141 gcaaagaagg taaaaagaaa taaggcatag atgtaaggat taaaacaatg tcaaaagcat
     4201 taacatcagc tcagcgcaga acgtatcgca cacgcaacaa aattcgccgt gtgaatatcg
     4261 accgtgttgg tgtgcgggaa acgcgccctc gcctgtccat atttcgttca ggaaagcaga
     4321 tctacgctca agtgattgac gacatcaaag gtgtaacatt ggctgcggcc tctacgcttg
     4381 aactggaaat gaaagaaaag ctcaaaaaag gcggcgcaaa tacgcaggct gctgctgaag
     4441 ttggtaaatt ggttgcccgg cgcgcgaaaa aagccggtgt tgaaaaggtg gtcttcgacc
     4501 gcggacgttt tctctatcat ggccgcgtga aggccttggc tgatggcgcc cgtgaaggtg
     4561 gactgttatt ttaggaataa gttaaaaaaa ggacgaataa aaaaatggct cgtggacaag
     4621 gacaagaagc agaacgcgaa gaaccggaat ttaccgaacg tttggtcggc attaaccgtg
     4681 ttgctaaaac cgtgaaaggt ggccgccgta tgggctttgc tgctttaatg gttgtaggcg
     4741 acggcaaagg ccgtgtcggc cacggtcacg caaaggcgcg cgaagtaccc gaagcgatta
     4801 aaaaagcaac cgaacaagcc aagcgcaata tgatccgcgt accgttgcgt gaaggtcgta
     4861 cacttcacca cgatgtaaaa ggccgtcatg gtgccggtaa agtctttatg cgctccgctc
     4921 cgcaagggac aggaattatc gctggtggtc caatgcgcgc tgtgtttgaa gctgtaggta
     4981 ttcaggacgt tgttgccaag gcaattggct caaacaatcc gtattccatg gtcaacgcga
     5041 catttgcagg gcttaaggcg atgcaaagcc cacgtcacgt agcctctcgc cgcaataaaa
     5101 aagttggcga tgtcgtttca ggtcgcggtg acaaagaagc ccatggtgaa gaataagggc
     5161 gaagaataaa gaattgaatt taaaggagtt ggatgatggc cgacgaaaaa gaaataaaaa
     5221 cgaagaagtc tgcagctaag aaagcgcctg cccaaaaaga ggcagcccaa aagcctgccg
     5281 ctaagaaacc tgccaaggct aaaaaggcag atccgaagaa ggaaacagca gcaaaagcag
     5341 ctccccaaaa agagacggct aaaaaggacg ttcctaaaaa agctcccgcc caaaagcctg
     5401 ccgctaagaa aacatcgtct ggtaagacag tgaccgtaac gcaaataggt tcacccatcg
     5461 gccgcaaagc ctaccagcgt gcaacattgg tcggtctggg tctcaacaag atgcaccgta
     5521 cccgcacact cgaagacacg ccttccgtgc gcggcatgat caacaaagtc aagcatctgg
     5581 ttaaagtcga agacgcggca taaaggattt aaaaggacca agctcatgaa actcaatgaa
     5641 ctcaagaaca atgacggcgc aaaaaaggcc cgcattcgcg ttggccgcgg tatcggctct
     5701 ggcaaaggca agacctctgg ctccggccaa aaaggtcaga aatctcgctc cggcgttgcc
     5761 attaatggct acgaaggcgg ccaaatgccg ctccatatgc gcatgcccaa aagcggtttc
     5821 aacaacaaga aattctgcgt ccattacgtc gaattgacaa caggaaaact tcaggctgcc
     5881 attgacgcta agaaaatcga ttccaagaaa actatcgatg aagacacact ggtagccgct
     5941 aaaatcatca gccgcaaaaa agcaggcgtc aagctgctga ccaaaggcga gttgaaagcc
     6001 aaagttgaac tgaaaatctc aaaagcgaca aaaacggccc gcgccgctgt cgaaaaagca
     6061 ggcggtaaga tcgaaatcat cgaaaaaacg gtcgcgccgg ttgaaaataa gaagaaaaaa
     6121 taatgtaacc ccaatgttgc tggatagatc aaaccccgcc aagtgtgcgg ggttttttgt
     6181 tcatcaattt aacaaatcct taaatctctt gactatacaa tggggaagca aaatatgcgc
     6241 aaagggcaga aatacacatg acgggcaggg caagaaacaa aatcaacata ctgatattat
     6301 atgcgttttt gagcattttg gtctatgctg cgcaggcatc tgcacatagt tcttctaaat
     6361 cacacaacca taaatcatcg caaagcgcac ctgaacaact ttcatgcaaa gcctcaaagc
     6421 cgtccttatc cgtaaaaacg gcgaccacac gcacgaaata tattcggacc aaatcctcca
     6481 aagacctcac acaacttcac ggaggaggag gttcatctgt tggcggtctc ggcggcggcg
     6541 aaataggatt caaaacggaa ggcaaattcg aaatcacgga agctgggggg aaggcttgcg
     6601 taaagctcaa acagcttgaa gttacatttt acgccaaacc agaaattcat attgccagca
     6661 attttaaccg ctcgacatgc gaacataatg ccgtaatggc gcacgaaaaa ggccatatcc
     6721 gaatcttgcg taaatttgtc cgtgaatttt caccgaaggt taaagttttc ttgtcgaaat
     6781 acgtgtacaa aatcgatccg gcgataggcc caattccgaa atcacaagtt aatgaagcac
     6841 aaaagaaaat tcaagaacaa tacatgtcca agcttgaagc ttatcaaaac aagataatgc
     6901 ccatacttgg caaacgccaa aaagcccatg atagcccaca ggaatatgct cgcgtcgctt
     6961 caaaatgcag caagtgggac aagaaactgg cgcaatagct gtttcgtttt tctttcaaaa
     7021 ccatctgtga tcaagcataa tttcagcgtc taagggactt gtgtgttccc cgacttttct
     7081 ttatgactgt gtctttaaac attcttaagt caaaaaagga ctgtccatgg cttcagccgt
     7141 cgaacaactg gccaaaaacg cgaattgggg cgctctggcc aaagctacag atttaaaacg
     7201 ccgtttgctc tttgttatgg gggccttgct cgtctaccgt ttgggaacat acatccctgt
     7261 gcccggtatt gacccgcatg tgtggaacga aatcttcacg caaaaaggcg gcggtattct
     7321 ggacatgttt aacatgttct ctggtggtgc gctttcgcgg atgactattt ttgcactgaa
     7381 catcatgcca tacatctcgg cctcaattat catgcaactt gcgaccgcta tgtcgccgca
     7441 gctcgaagcg atgaaaaagg agggtggcac cggccgcatg aaaattaacc aatatacccg
     7501 ctacctcact gttttgttag ccactgtaca ggcttacggg ctggccgttg gtctggaaag
     7561 catgcaaggc tccaccggtt ccgccgtgat tgatccgggc ctgtttttcc gtatctccac
     7621 cgtcatcacc attgtcggtg gcaccgtctt tctgatgtgg ctgggtgaac agataaccgc
     7681 gcgcggcata ggtaacggga tttcgctgat tatctttgcc gggattgttg ctgaattgcc
     7741 gcgcgctatc gcttcaacgc tcgagttggg ccgtcaggga acattcagcg gtttagaact
     7801 tttggctcta tttttgatga tcgcgggtgt gattgttttc atcgtcttta tggagcgtgc
     7861 ccagcgccgc gttatcgtgc aatatcctaa acgccagcaa ggtaatcaaa tgatgggagg
     7921 tgaaaccaac cacattccgc ttaagctgaa tacagctggt gtgattccac cgatttttgc
     7981 atcgtctttg ctgctcctgc ccctgacaat tgtaggtttc agcggtgcag aaggtggcga
     8041 tttcaccgct acaatttcgc aatatcttgc ccatggtcaa ccaatctata tggcgctcta
     8101 tggagcactg attatgttct tctgcttttt ctacactgcg attgtattca accccgaaga
     8161 aaatgcagac actttgcgca aatatggcgg ttttattccc ggcattcgcc cgggaaaaca
     8221 taccgccgat catctggatt atatcctgac gcgcattacg gtcttgggtg cagcctatct
     8281 ggtgtttatt tgtctgttgc cggaatttct gatttcccaa tacaatatgc cgttttattt
     8341 cggtggaaca tcgctcttga ttgtggtgag tgtgaccatg gatacggtcg cacaaattca
     8401 ttcgcacttg atcgcgcacc aatatgaagg cctaatgaag aaagccaagc tgggtaaacg
     8461 tcgcggcatg atgcgcggca cgcgttagaa aaaataaaag gaacaacaaa tgaacttgat
     8521 tatgttcggc ccccccggtg ccggtaaagg cacacaagcc aaactcattc aggacaaata
     8581 ccgtatcaaa cagctttcga ccggcgatat gctgcgcggg gaggtagctt ctggctccaa
     8641 tatgggcaaa cgcctcaagt ctattatgga ttccggcgaa ctcgtctccg acgaaattat
     8701 catcgaaatc atcgccaact gcatttccga acctgaatgc gaaaaaggct tcatccttga
     8761 cggtttcccg cgcacagtcg accaagccga agtcttggat aatatgttgt cccatatggg
     8821 ccgcaagctc gatcacgtca ttgtgctgga agtcgatgaa gaggccctga tcgaccgcat
     8881 ccagacccgt gcggctgaaa ccgggggcca acgcagcgat gacaatgccg aaaccctcaa
     8941 aaaccggttg aaagtctata atgatcagac cgcgcccgtg ctgccatatt acaaaaatca
     9001 cggcattttg cgccatattg atggtatgca atcaatcacg gacgtaacgg cccagatcga
     9061 cgccgtttta aaataagctt tcgttgcgca aggtcaggct ttaccgtgcg aattatttag
     9121 ttgacgcgag gaccaaaatc cctatattgc acccacaatc ccattattgt gggtgttttg
     9181 gtttatgaac cggctggtga aagcttaacg cgcggagcca tttacggttc agttgaaatt
     9241 gaaaggaaaa tgaaatggca cgtattgccg gggttaacgt acccgacaaa aaacgtacgc
     9301 cgattgccct gacctatatt catggcattg gccgtaccac cgcttttaaa atctgtgaca
     9361 aattgggaat cgatgtacaa aagcgcatgg gtgaactgga cgaagataca ctcaatcgca
     9421 tccgcgccga aatcgacacc gacgcctata tgatcgaagg ggatctgcgc cgcgagacat
     9481 ccatgaacat caagcggttg ttggacatga aatgctatcg tggcctgcgc caccgtaatg
     9541 gcttgtctgt tcgcggtcag cgtacaaaaa caaattgccg tacgcgcaaa ggccctgcaa
     9601 aaccgattgc cggtaagaag aagtaaggag ggcgtaaaaa atggctaaac caaagacaaa
     9661 agcaaaaaga aaagagaaaa agaacatcgt ctctggcgat gtgattgtaa actcaacatt
     9721 caacaatacg ttgatctcct ttaccgataa gcaaggcaat gttgtgtcat ggtcttctgc
     9781 cgggatgatg gggttcaaag gctctcgtaa atcgacgcct tacgctgccc agatggcggc
     9841 tgaagatgct ggccgcaagg cgcaggaaca cggtatgaaa gaagttgacg ttcgcgtcaa
     9901 aggccctggc tcaggccgcg aaagcgcact gcgggctttg caaaacatcg gctttacgat
     9961 caagcagatc aatgacgtga cgcctattcc gcacaacgga tgccgtccgc cgaaacgccg
    10021 ccgcgtgtaa ttgccgaaga cgtccccccg attaccaaaa ggagttggtg gggcctcttc
    10081 aaagaagatt ttttaaccca actaagaggc cggctaagaa ccggatgaag tgagaaggaa
    10141 aaaccaaatg atacagaaaa actggcaaga actgattaag ccaagcaaac tcgatatttc
    10201 acactcagaa gttcccaaca ccgggaaagt cgtcgcagaa cctttggagc gtggctttgg
    10261 cttaacgctg ggcaacgccc tgcgccggat cctgctctca tctttgcaag gcgctgccgt
    10321 tacagctgtg caaatcgatg gtgtattgca cgaattttcg tccatagaag gcgttcgcga
    10381 agatgtcaca gacatcatcc tgaacatcaa agctttggct gtgcgcatgc atgccgaggg
    10441 accgaagaaa atgcgccttt ccgcagaagg cccttgcgaa gtcaccgccg cacaaatcga
    10501 agcgggtgct gacattgaaa ttatgaaccc tgatctggtg atttgcacac tggacaaagg
    10561 tgccaagctc tccatggaaa tgaccgtgga tacaggcaaa ggctaccgtc cggctgcaca
    10621 aaaccgcccg gaagaagccc ctgtgggcct cattccggtc gacagcgtgt tctcaccgat
    10681 ccgcaaagtc acctacgaag tcgaagatac tcgcgttggc caaatcaccg actatgataa
    10741 gctgactttg acattgcaaa caaacggcgt tatcacgccg gaagatgctg tggccttcgc
    10801 ggcgcgcatc ttgcaggatc aattgcaagt ctttgtgaac ttcgatgagc cggaagtggc
    10861 caccgagaaa gaagaagagc atgaattgcc gttcaacaaa aacctgctga aaaaggttga
    10921 agagcttgaa ctttctgttc gctctgccaa ttgcctgaaa aacgacaaca tcgtttacat
    10981 tggggatctg gttcagaaaa gcgaaagcga catgcttcgt acaccgaact tcggccggaa
    11041 atctctgaac gagatcaagg aagtgctgac catcatgggc ttgcatctgg gcatgcaggt
    11101 tgaaggctgg ccgccggaaa atattgaaga tctggccaaa aaggcggatg atcaacaatt
    11161 ctagggtttg atgtggggct gcggcattgc ggggccccat ttaaaaatag gggtgagtac
    11221 cccacatgcg aacaaggccg ggaaaacaac atactctcag ctaatctctc cccagagggt
    11281 ttgcattatg taaaatggct ttacccgttt aaagtcgcag ccgcgccaaa ggtggtgcag
    11341 aaaggacgat tcgatgaaac atcgtatcaa acaacgtaaa ctaaaccgtg actccagcca
    11401 ccgtaaggca atgctggcaa atatggcggc aagcttggtt aaacatgagc agatcactac
    11461 gaccttgccc aaagccaagg aactcaagcc ctatatcgaa aaactcatca cattgggtaa
    11521 acaagccagt gccaacccgg ccactgcact tggaaaacgc cgtcaggcca tctccaaaat
    11581 gcgtgacgaa gctatggtta gcaaaatctt tgatgtcctg gctgagcgct acgaaggacg
    11641 ctctggtggt tatacccgcg ttctgaaagc cggtttccgc tatggcgatg ccgcgccgat
    11701 ggcggtaatc gagctggtcg atcgtgacgt cgaagccaaa ggcaaagatt ctggtcctgt
    11761 tttcgaggac atgccggtgg gagcgtccga agaattgaaa gaaaccgtag caaagaataa
    11821 agaaaaagcc gaaaaagcta aagccgagaa aaaaccagcc gagaaaaaac cagccgagaa
    11881 aaagaaagtc actcctaagg ctgaaaaagg cgaaaaagcc ggagcaaaaa aagccgcgcc
    11941 taaaaaagcg cctaacaaat caacaagctc taagcgcggc gcctaaacct aaacgtcaaa
    12001 gcgttataaa ttcaaagaaa agcgggccgt tacgggcccg ttttttatgt ggtcgaagac
    12061 agaacaccac ggctttagcc gtggatgaat gagcccggag gcggtatgtt gtacaggagg
    12121 tagtccctct tgccttaagc gcttcccaat tctggcgtct ctttttcata taaacttgca
    12181 attttttgcg ttttctggta ccatgcgcgt aaatcaagga gaagattatg agtgcttcac
    12241 ttataccgat gaatgatccc tctcaggtca aggggccgga tggtaaggtt gatcagaatt
    12301 ccatctacct gttccaacgc ggtattacaa atgccactgt tgatgctaga agacaaggag
    12361 atgaaagatt ttggtcttgt taccttactt tgagccttgc acaagaacct ttcttttcac
    12421 cagggctaga caagcctgaa catgacctgc attgaattaa atagacatca aggttgaaag
    12481 gcgcttaaat aagcgccttt tttaatgcct tagcgaatca cgatgcgttg caggcgcccg
    12541 ccgctttgaa tgaccagcga gagtccctgc ccgcttccac ggttaagcgc tttttcgatg
    12601 tccttcacat cgttaatctt gcggccattg atctcaacca gcagatcacc gggtgagagc
    12661 acccgcgcgg cgcggctggc gcgcgcaact ttcagcacaa caatcccttg tgcatcgctg
    12721 ctcagaccca gctccagcgc aacggccgga ttgacgttac caaccaccgc gccattcagc
    12781 gcgtgctcgc cgcttaaaga tgtttcatcg cgcggcggat cctcaggcgg agccatcgcc
    12841 tcgacataga cattcagctt ctggtcctga cgcattagcg tgaccttagc cttatcgccc
    12901 aggggcacgg tggccatgcg gaatttcatt tctgaggcgt cacgaatttc cttatcattg
    12961 agcgaaagaa taacatcacc gactttcacc ccggcttttt tgagcgggct ggccgggtgc
    13021 agtgcattga tcaatgtgcc gtgcggcgtt ttgaggccca gtgaggcagc aatgtcagcg
    13081 ctgagatttt gcgaggtaac accaagccat gggcgaatta caccgcgcgc gccgctttga
    13141 ccggcttttt ccgcagcgat tacgctggcg accatttcgg atggaatcgc aaagccgatg
    13201 cccagcgagc cgccatcacg ggaataaatc gcagagttga ttccgaccac gccgccatcc
    13261 agtgcgacca gtgcgccgcc ggaattgcca gggttaatcg ctgcgtcggt ctggatgaag
    13321 aagttaaagt cgttaatgtc gagcgaagag cgcccttgtg cagagacaat gccgctggtg
    13381 accgtctggc cgacaccgaa cgggttgccg atggccagca ctatatcacc aacttccagc
    13441 gtttcgctgg gcttgagaga tacataaggt aaggattcgc cgcgaagatc atcaatgcgc
    13501 aacaacgcca aatcagatgg ctcatcggcc agtgcaaggc gggcctcaaa ctcccggcca
    13561 tcactgagca cgaccgtaat ctcatcggca tcccggataa catgggcatt ggtgaccact
    13621 aggccgtcgg aggcaagaat cacgccggag ccgagcgcac tttcgatttg tttgcgcatg
    13681 cgtccaccga aaaagtcatc gcggaagaag ggtgcaaaga agggatcgcg agcgaaggga
    13741 tgcacgcttc tttgtgatac ggtacgtttt gtatatatat taacaacggc aggggcggtt
    13801 tgtttaacca gcggtgcgaa ggagagctgg atttgctgct ggctttgcgg caacagcttt
    13861 gtttgcgccg ccgcagggtt tacgtttacg gcgcaaagag ctagcaacag tacgaaaact
    13921 ttggtaagca taatttggaa tttagggcgc attgtccaaa atctcaagag gccagatgag
    13981 aaaaacgatc gaagaaatca aatcccataa aaaccagccc gagccgctgg tgtgcctgac
    14041 cgcctatacg gcgcggattg cgcagatcct tgatccgcat tgcgacttgc ttctggtcgg
    14101 cgattctatg ggcatggtgt tgtatggtat ggactctacg cagggcgtat cgctggagat
    14161 gatgatcaat catggccggg ctgtgacctg tggctccaca cgatcctgcg tgattgttga
    14221 tatgccgttt gggagttatg aagagtcccc tgcgcaggcc ttaaacaatg caaggcgggt
    14281 catggatgaa accggagcgc agggcgtaaa gctggagggt ggggtagaga tggccgcgac
    14341 gattaaaacg ctcgtcaagg caggcattcc ggtgatcggg catattggcc tgatgccgca
    14401 atcggtgact aaagagacag gttttagggt taaggggcgt tcggctgaca gcgaggcgca
    14461 gcttttggct gatgcaaaag cgattgaagc agccggcgtg agcgcgtttg tgattgaggc
    14521 gacgattgag tctgtggcag cggaaattac gctggctgcg ggcgttccaa caatcgggat
    14581 tggtgcctcg gctgtgtgcg acgggcaaat tctggtgagc gaggatatgg tcgggattac
    14641 cagcgggcat gtcccgaaat tcgtcaaaca atatgcgcag gttggcgaga taattggaga
    14701 tgccgcggcg cgctatgccg ccgatgtgcg ggcacgggcg tttcctgctg aagcgcagct
    14761 ttatggcatg agcgcagccg gaaaggttaa aaaggcctca tgagcttaag cgttcaccat
    14821 acccagaaag atttacaggt ccttgtacaa gactggaaag cgcaaggcct acgcatagcg
    14881 tttgtgccga cgatgggggc gttgcacgaa gggcatttga gcctgatgcg gctagcgcgc
    14941 gggaaagccg ataaggtcgt tgtaagcatt ttcgttaatc caacacaatt tgccccgcat
    15001 gaagattttg atacctatcc ccgtgatctg gaaagcgatg tggaattgct gaagtctgca
    15061 ggcgtggatg cggtttatgc gcccacagaa ggcgatcttt atcctgaggg cgcggtgagc
    15121 gatcaaaaag caggtgcggc ggcagcgggt ttagagagtg atttccgccc gcatttcttt
    15181 gacggggtgg tgagcgtagt ttaccggctt tttaaagccg tggagcctga tgtggcggtg
    15241 tttggcgaaa aagattttca gcaattacag gtgatccacg agatggtagt ggataaggac
    15301 atgggtatcg agatccttgg cggaccgatt gcgcgggata aattcggcct ggcgctctcc
    15361 tcgcgcaatg cgtatttggg cgcaggcgag ctggagattg ccagaatgct taatcaggtg
    15421 gtttacaagg cagcagagaa cccaagtaat gaagctctgg aagaagcggc ggcgatgctt
    15481 gttgaggcag ggtttgataa ggtcgattat gtagcctata ggccggaatg gaagcgcgta
    15541 cttaccgccg catggctagg caaaacgcgg ctgattgata attgcggggt ttaacttggg
    15601 ctcaggacct ataaacatta acgattcaca gcaggtcagg aatatgattt actgatgata
    15661 cccccctcat cacaagtgaa tcgcatacca actatcttgt gaattcataa tcaataagtt
    15721 ctgagcctgg aatttttatg cggcttcagg ctccagactc tcttcttccc cttcctcctc
    15781 ttcttcctca taagaccaaa agagcgaatt attgctagct gccatattga acacgcccac
    15841 atcaaagtca aaagcgctta cccactcagc gtcttcaaaa gcctcgatca ttggatcgcc
    15901 tgtatgaaca tgctcgccgg tcatatcgct tgtgttgcca ctggtgatct cctttttgac
    15961 ggtatcacgc atgtcgggag tcacctcttt gtaccctgca acagaagagg gcgcatgcgt
    16021 gtgttgcata atactagaca tagcctcctc ctttctcagt tattgtaaat aaatacagta
    16081 aaaacataaa aagtcaagaa aaataaggga taaatatgta tatacagagg atttatataa
    16141 tacaaaaaca aacaatattt atacatatac aaaaacaaaa tagaattatt aagaaataat
    16201 actttaaatt aatactattt aacttataaa actaaattta ttaagttatt ttttcttcgc
    16261 tctgtttaat aaaatcagtt attcttccgc cgtgcttgcc aagcggggcg ggttgaaaaa
    16321 cgcttgtttt tgatgtgcaa tcaggccatt gtggggcatt attcacacag gattgctgct
    16381 ctgcgcacct ccatggtgaa taaaaccaaa gtaataaaat ttatatgggt atttgaggag
    16441 atttttcgtg cctgttgata tgaaagacat cgtcgcgctg tgtaaacgcc gtggatttat
    16501 ttttccagcc agtgatattt atggcggcct gaatggtttt tgggatttcg gcccgctggg
    16561 cgtggagctg aaaaacaacc tgcgtgaccg ctggtggaag gcgatggtta tcaccccgcc
    16621 gctcggcccc gatggtgcgc cggtcgatat tgtcgggatc gattcggcca ttatccaaaa
    16681 ccctaaaacc tgggtggctt cgggccatgt cgggggcttt tcagacccga tggtcgattg
    16741 tagggaaacc aagaaacgct accggcagga tcatctcatc gtcctgactt cggaaaaggc
    16801 cgaccgctgg cccgcattcc acgaagaaac cgaagattcc gaaatggaga aaaagctcaa
    16861 aaagcttggc atgccttccg atctgtcatt atttgaaaag atcaatttga ccaaattgcc
    16921 gatcgaagat tacgcgaaga ttatcggccc ggacaccaaa gagcctggca cgctgaccga
    16981 gcctaaagcc ttcaacctga tgtttgaaac ccatgccggg ccgattcaaa gcgaagaaaa
    17041 caaggtttat ctgcgccctg aaaccgccca aggcatcttc ctgaattaca aaaacgtgct
    17101 cgattcaacg cgcgtgaaaa tgcccttcgg gattgctcaa atcggtaagg cctatcgcaa
    17161 cgaggtgaac ccgcgcaatt tcatcttccg ctcgcgcgaa ttcgagcaaa tggaaatgga
    17221 atggttctgc caccccgatg aggccaaaaa atggcaggaa ttctggtttg atgagcgcgt
    17281 taaattctgg aaatccgttg gcctcaccga aggcaatatg gagatgcgta aacacgatgc
    17341 cgatgaattg gcccattatg ccaaggaagg tctcggcacc tcggacattg aatataagtt
    17401 ccccttcacc gatccgggct ttggcgagct tgaaggcatt gcccaccgct gcgatttcga
    17461 cctgaaatcc catcaggaac actccggcgt caagatggaa tatctcgatc ccgcccgcga
    17521 taatgagcgc tatattcccc acgtgatcga accggctgcc ggcctaacgc gcggagtgct
    17581 ggcactgata tgcgaggctt acacagtgga tgaaaatcgc ccttccggcg tctatctcaa
    17641 cttcaccccc gcaatggccc cgaaaaaggc cgccattttg ccgctgaccg cgaaggaaga
    17701 gcatgtaccg attgcacaga aactctatct ggaactgcgc gaggaattcc cggtcgatct
    17761 cgacatcaaa caa
//
LOCUS       DAOSWR010000029        17673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2027_length_17673_cov_5.048757, whole genome shotgun sequence.
ACCESSION   DAOSWR010000029 DAOSWR010000000
VERSION     DAOSWR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_17673_cov_5.048757"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="xrt"
                     /locus_tag="PK513_03615"
     CDS             complement(<1..1078)
                     /gene="xrt"
                     /locus_tag="PK513_03615"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HOO81571.1"
                     /translation="MLTFLPIYGGLWNSWQRDEYSHGILIPVIALLIGWHRLTKSKPV
                     PAPSWTGLVLIFTGFIFLILSKLSAFMPPAYYGFILCLAGLVLSFGGLQSFRVLLPAL
                     IYLAFAVPLPRLIEVALTAKMQLLSSTLGVIILQFLGISVFQDGNIIDLGQAKLQVVE
                     ACSGLRYLFPLMSLGFLIAFLFNGTFWKRALIFLSTIPLTLGMNSLRIAVTGILVNIF
                     GPGAAEGIMHDAEGFFVFGLCLALLFIEVWLLSRSKNKPCGINFDYLRIPSGNIFGGK
                     PAVATPALATVVLSLLMACVLLVANPDNRENQIPNRESFHSFPLSLEQWEGHTIAMAP
                     DLVKTLGATDYWNASYTRSNIPGNV"
     gene            complement(1162..2268)
                     /locus_tag="PK513_03620"
     CDS             complement(1162..2268)
                     /locus_tag="PK513_03620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO81572.1"
                     /translation="MKVAVLHDWLETYAGAERVLEQILKLYPEADLYALVDFLPNDKR
                     DFIGGRRAKTTFIQHLPLARRYFRHYLPLFPMAVEALNLSAYDLIISSSHCVIKGVRV
                     RDDQTHICYCHTPVRYAWDMQEEYLSTTGQGTGLKGKLIRFFLRRLRKWDKKTAPRVT
                     SYIANSNFIAERIKKFYGREAAVIHPPVAVQDFEACANKQDYYFTASRMVPYKKFPLI
                     AQAFAKMPERKLVMIGAGPEAHKVAAIADKAPNIEYLGYQPFPVLKEHMQKARAFVFA
                     AQEDFGIMPVEALACGTPVIAYGKGGALDSVTPETGVFFDEQTPESIIAAVEAFEKKE
                     ETITPEACRKQAEKFAKDIFAAQMRKHIKKVLGT"
     gene            2368..2856
                     /locus_tag="PK513_03625"
     CDS             2368..2856
                     /locus_tag="PK513_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81573.1"
                     /translation="MAKHPLYINGRFLQKPVTGTERFAREIVWAMDELLAEQTDRPDV
                     TIISPPGTLAPEGLKVIGFETCGSRQGHAWEQWDLYRKTRDGVLLSLTNSGPVLHPRQ
                     MVVIHDGAPYRMPGDFSSAYRTFHRILGCLLVRQTRIATVSEFSRRDLAAVLGVSAEA
                     IP"
     gene            2922..3446
                     /locus_tag="PK513_03630"
     CDS             2922..3446
                     /locus_tag="PK513_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOO81574.1"
                     /translation="MKNRPYFLFVGSPVPRKNLDMATRAFHALEREDAAFVIVGASSA
                     KVFGKRTQTQAQNVILPGRLTDQEIVALYRHARALVFPSLYEGFGIPPLEAMVQGCPV
                     LASNIPPVREVCGDAALYFDPRDPADCMRRMREFLDDLSAREDLIRKGTARYPAFSWK
                     TSAGKMLDELMDMG"
     gene            complement(3608..4765)
                     /locus_tag="PK513_03635"
     CDS             complement(3608..4765)
                     /locus_tag="PK513_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO81575.1"
                     /translation="MQNLRQIAKSIYYFFFAKIAHTALRKSKAQDEKTHSKKTINIVC
                     RFRVQNGLTNGANYHMCALEKLGYDVRRIDITPAIRNPLKKIEGSKQDIYIFHCAAPQ
                     FLQQAWPLRESLKESGKRIGYFAWELPEPPVNWPVYDDVWDEIWTTSSFSAQGLGKLY
                     KCPIHVVPHVMEKYSPAPRVWKKGQESLTFLTMADARSGLTRKNPAAAVDAFQKAFPT
                     KQDVSLIIKLQGSTDIPEIKAFVESVTKDSRIKVLMKTLSREEIDELITKSHVFVSLH
                     RAEGFSLPLLEARMAGLATIATAWSGNMDFMNENDSILVPARLVEMFDESGVYGTVTW
                     ADPDTDFAARAMRRMYDDPEKMAAIARAGWEASSPEKQLEEYEIAIKKAGL"
     gene            4894..6198
                     /locus_tag="PK513_03640"
     CDS             4894..6198
                     /locus_tag="PK513_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOO81576.1"
                     /translation="MKSSSALLIFILIFTFFAFVAQAQQTISGVPAKSADDFLNSLGI
                     NTHVAQGYNPKNYIEPLKYTGIRNIRDAPKNLSGYFMLHKETGVRVNLVGAKLHHLIH
                     AAQTLAKHNILMSIEGPNEPNNWPITYKGEKGGGKGSWKVVAEFQRDLYKAVKADPLL
                     KDYPVFNISEGGAQTDNVGLQFLTIPEGAGTLMPAGTMYADYANAHNYVSGNGGRHVD
                     NQAWNAADPTLDSFWDGLYGNYGRTWRKKYKGYSNEGLMRLPRVSTETGWATKKGVSE
                     ERTQGIMLVNTYLAQFKRGWTYTFIYQLGEAQGSKGLQGIYRKDWQPKAAAHYIHNLT
                     TVLADTMSGDFKPGRLAYRIPNQPDTVHDLLLQKSDGRFFLVVWGERVKGQDDIIIQL
                     AKPAAKIKIFDVTEGSEPQKMLSDVLEIPLTLNDHALILEFH"
     gene            complement(6145..7536)
                     /locus_tag="PK513_03645"
     CDS             complement(6145..7536)
                     /locus_tag="PK513_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81577.1"
                     /translation="MNTVSVIALLIILGSIILSPTILLASVFICSSFSAMTVLPGNVV
                     PAMLASIIFLFRIGISQNFVPSFLAALFQPRQLGFLGIFWLISLIGAMFLPRLFNGQI
                     FTFSLNTAELEPVRPQGTNYTQTAYLTMSVFMAASVCILLQKKHEFLDALLKAIFAGG
                     YAIIATGLIDIIANLLGKADMLKIFYTANYGYSAGSILGTRRVLGLTPEPAAFGGLAM
                     TMASLLLFLRPVYAEKMRRFWVPLLGWSCMAMGFLSTSSTAYASLFLMLGLYLFYNVD
                     AVSEEGKILHKSITKKMLNIVFLAVCFLLAVLLSGNFVDLINTLLIEKTGTSSYIERS
                     SWTAAGLNAFIESYGLGVGIGSVRTSNFFANILASTGLPGALFFSLFLISLYLRHAPK
                     RNSREEAVIKAIKFALLPMFAAKFLAGTTPDFGVLQGILFGTIIALSQSNDSLDLMKL
                     QYQRMVIKRKRNL"
     gene            complement(7549..8586)
                     /locus_tag="PK513_03650"
     CDS             complement(7549..8586)
                     /locus_tag="PK513_03650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOO81578.1"
                     /translation="MSYKSEQVYPSNVHNASQRIDWPDAARGLCIMLVVIGHVIGGLR
                     DAQLVPIDGILVQIFNYIYTFHMPAFFFLSGMFASSLEKKDGKKFAKDLLIRIYYPCV
                     LWSAVQTFSMNLTGSLLNHPQDFSWFQIRNIALGNVSQFWFLKALILMQIVHYLFSRF
                     MNKKYELVFFLLLLGLSTFPALPSFFTPFAKFGIYYGLGTLLFQDKEKKEWTDLSFLF
                     LTLVAGILWFVVSSYSFTQTNLAEIGTPKYLSQVPSTILGCAFIFFLVRISYIRSFSA
                     LVYIGQRTMPIYVLHVMIVAGTRIFMVKGLGYSDPLVIFPIALILGTIIPVIFYDMAR
                     RFNLQRWLGLG"
     gene            complement(8586..9362)
                     /locus_tag="PK513_03655"
     CDS             complement(8586..9362)
                     /locus_tag="PK513_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOO81579.1"
                     /translation="MSVFYPPGLEVNNKYLKRIPYSQNVKILKTKLKFKLHAQDNPIT
                     HFSKAEAFFIRIPKNASTSLSQFLFPETKYELPHYSAEFYKTLFPNYYSKWLVFAPLR
                     NPLDRIASAFTYYRSYTKVPAEKKLMEEEYSFLKTFEDFVYWLNDIEHIEEQEIMRWS
                     HFRKQTDFICDTNGDIIVDLLFPIEDMKKGIEFLRKRYKATGEIPHNNKSTTQKHDTL
                     PTKPIERYFSEDFKVWSQAINDKIIFNHNNKPGLNLPKEK"
     gene            9599..10510
                     /gene="cysD"
                     /locus_tag="PK513_03660"
     CDS             9599..10510
                     /gene="cysD"
                     /locus_tag="PK513_03660"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOO81580.1"
                     /translation="MAAMNTLTHLQRLEAESIHIMREVVAECRNPVMLYSIGKDSAVM
                     LHLARKAFHPSKLPFPLAHVDTTWKFRDMITFRDKMAQEAGAELLVHINEEGVKQGIG
                     PFTHGSALHTEIMKTQALKQMLDHYQFDAAFGGARRDEEKSRAKERIFSFRSKAHRWD
                     PKNQRPELWNLYNCRKHDGESIRVFPLSNWTELDVWQYIYLENIPVVPLYFAAERPVV
                     ERDGTLIMVDDDRMKLEPGEKPAMKKVRFRTLGCWPLTGAIESSADTLPAIIREMLLT
                     KTSERQGRVIDNDSNASMEQKKQDGYF"
     gene            10510..12399
                     /gene="cysN"
                     /locus_tag="PK513_03665"
     CDS             10510..12399
                     /gene="cysN"
                     /locus_tag="PK513_03665"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HOO81581.1"
                     /translation="MHSDSALIEEDVEEYLRAHEHKTLLRFITCGSVDDGKSTLIGRL
                     LYDSKMIFEDQLAALESDSKKVGTQGNSIDFALLVDGLAAEREQGITIDVAYRFFSTE
                     NRKFIVADTPGHEQYTRNMATAASTADVAIILIDARKGVLMQTRRHSFIASLMGIKHV
                     VVAVNKMDLIGYDEAAFTAIKEDYRAFAKGLDIEHITCIPMSALGGDNVMQRSAKMDW
                     YDGPTLMAYLETVEVEDLAQDCPFRMPVQWVNRPNLDFRGFSGQVAAGRIEPGMEIKV
                     MPSGKTSRVSKIVTFDGELPEAVASQSVTVTLADEIDISRGDMICAAEHPAEITDQFE
                     AYLIWMGDEKMLPGRPYIMKTGAHQVPVSVTKLKYTINVNTMEEHAAHSLVLNEIGVC
                     NFSAERDFPFDIFRDIPGTGSFILIDRITNATVGAGTINFALRRASNIHWQALDVDKN
                     TRAALMHQTPRVVWMTGLSGAGKSTIANLVEKKLHSLGKHTYTLDGDNVRHGLNRDLG
                     FTDAQRVENIRRVAEVAKLMTDAGLIVLVSFISPFRAERRMARRLFEDGEFIEVFVDA
                     SLETVESRDPKGLYKKARKGEIANFTGIDSPYERPEYADLTIDSNKMSAEEAAEKIVA
                     LLMDT"
     gene            complement(12409..13101)
                     /locus_tag="PK513_03670"
     CDS             complement(12409..13101)
                     /locus_tag="PK513_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81582.1"
                     /translation="MITLHQLKQKVSMAVISFKHNFAFVKTHKTAGTAIEVSLSPLLP
                     QSDILTEIKPKEEGHKARNHKRLFKQKIYNHMSSSEIKNFLGKEKSMELTFFCIEREP
                     IDKCISHYCMLRYSSYHNKDSSYKLSWEEYVEKGDFPEDVSLYSKRNETGDLELDVDL
                     VLPYENLEKSLQGFFSSKGLMGFSMTSKAKSGFRKQSEEVPSIDTVKPSHKEKIYKAF
                     EDSLKLAQLYNT"
     gene            complement(13169..14098)
                     /locus_tag="PK513_03675"
     CDS             complement(13169..14098)
                     /locus_tag="PK513_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO81583.1"
                     /translation="MIYPYVIIATKGRAKETYVLLDYLARQTLTPTMTLVVGAEDQDI
                     AGLNDHPMALSGSYEVFVSTKIGLTCQRNAGIEALKTQGAFNGEPNSYFCTFFDDDFR
                     PAPDWLEKAATRFAKGDIVGLTGRVLADGVMSGGYSEEEAAAFLSEQKPPLKHWANQN
                     HESETDCAYGCNMAFLGTAISALRFDENLPLYSWQEDRDFTGQCLRLGRVIRYPDCIG
                     VHMGATGGRTSGLRFGYSQIANLHYLIRKGTVPFRAGFMQAVRNFIANILKTLIPIRR
                     DVDYTGRLRGNLMALFDLSRGRCDPRRITQKEF"
     gene            14266..15069
                     /locus_tag="PK513_03680"
     CDS             14266..15069
                     /locus_tag="PK513_03680"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOO81584.1"
                     /translation="MTANNNRPIHNLVKDCLGFAVSNINGQCEFSKSIFVIAHMRCGS
                     TALSNVLTNHPSISGFGEAHIEYGSRSKLGNLVIRQIKEKSWKHKSDYLFDKILHSSY
                     DKNVCEDFYNSKAIFLAREPEASILSIRNLFERINSKDFCTDQLAADYYIERVSDIIK
                     TWHRFEARNRLGLTHSDLVSGPDAQLNRISAFIGISPPLENKYESRSASTKEGAGDPL
                     SSDKFNKILADGDGTSLSKTRNLEITESTHEKIQSVYKKFIRIIRDDPV"
     gene            15194..16708
                     /locus_tag="PK513_03685"
     CDS             15194..16708
                     /locus_tag="PK513_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOO81585.1"
                     /translation="MSFDSKEIPFSHKIANGGSLTIAGKLVCRCLDLITLAILSRLLS
                     PADFGLTALAMTSVIIAETVTSLPLAAALLRVSKPSRAMYDTAFTLAFLRGVLLAVVL
                     SALGFVMMYIYNEPRLVALVAALSIAPILRGLLSPRMVDYMREMNFMPEAGIEIAGKT
                     AALVASCAVAFSTHSYWAIAVSTITTPFVMNLLSYRVAPYRPRFCLSEWVLFKNYVGW
                     NSLTQLIGALKWQMDKLLLGYFVPKATLGQYTMASDLNNIPLQSIVVPLKRPVLASYS
                     ARGVRPPEELMKTYLKTSNAMLAFAGPVFVGLALLAVPAVYLVLGPKWEEAGSLLQWI
                     AIAALLTLPVINLDQLALALDEARMLTWRTVLEFCVAVPLLFFGCVTYGVMGVVGARI
                     IGGLFTVLISMEAVRKLIGCSFACQIMVLQRTVFALFLMGGSLWFLRPLVIMDDSVIA
                     LAFSLAGVGLGGLLFYIVSIAIFWRLAGYPAGIEAVVWGKGENILSQMKKRLVS"
     gene            complement(16715..17200)
                     /locus_tag="PK513_03690"
     CDS             complement(16715..17200)
                     /locus_tag="PK513_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOO81586.1"
                     /translation="MTLNTAINRVQRYACDLEAYKIGDSDTRPWGSYVVTGVGPLKDG
                     SEYCEKTITLMPGKILSLQSHELRTELWRVEEGTLTVVLNGQRLTLNAGDMINIPLQS
                     VHCMANLTSKPCKIYEKQTGICREEDIIRYVDAYGRGTQTGAAEDSLTLYREILDEIK
                     S"
     gene            complement(17335..17634)
                     /locus_tag="PK513_03695"
     CDS             complement(17335..17634)
                     /locus_tag="PK513_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81587.1"
                     /translation="MGPPLNALAVVNEIAAAWWTAQGYTIEEKDGVKQLIGKNAKTGE
                     DEPDKARTLTWAKIEESPDGTFYIASPINKPAFAKWRNHLPKGVTMPEDKEFSKS"
BASE COUNT         4515 a         3894 c         4399 g         4865 t
ORIGIN      
        1 caacattgcc cggaatattt gaacgggtat agcttgcgtt ccaataatcg gtcgcgccca
       61 gtgttttcac gaggtccggc gccatcgcta tcgtatgacc ttcccattgc tccagtgata
      121 gaggaaaaga atgaaaagat tctctgttcg gtatttgatt ttcccggtta tcagggtttg
      181 cgacaagtaa aacgcaagcc atcaaaaggc tcaggacaac cgtagcaagc gctggcgttg
      241 cgactgccgg ttttcctccg aagatgtttc cggaaggaat ccgcagatag tcgaaattta
      301 ttccacaggg tttgtttttg cttcgcgaaa gcaaccatac ttctataaaa agaagagcta
      361 aacacagccc gaaaacaaag aatccttccg catcgtgcat gatgccttcg gctgcgccgg
      421 ggccaaatat attgaccagt attcccgtta cggctattcg caatgaattc attcccagcg
      481 ttaagggaat tgttgaaagg aagatcaacg cgcgcttcca gaacgttcca ttgaacagaa
      541 aagcgatcag aaaacccaga ctcataagag ggaaaagata gcgaagaccg ctgcatgcct
      601 cgacaacctg aagttttgcc tgacccaaat caataatgtt tccgtcctga aaaactgaaa
      661 tgcccaaaaa ttgtaaaatt atgacaccga gcgttgaaga caaaagttgc attttcgctg
      721 tgagcgctac ttcaatcagg cgcggcagcg gaactgcaaa ggcaaggtat attaaagctg
      781 gcaataatac ccgaaagctt tgcaagccgc cgaaagacaa gaccagtccg gccaagcaca
      841 gtataaaccc gtaataagcg ggcggcatga aagcggagag cttggagaga attaaaaata
      901 tgaatcccgt aaaaatcaga accagacccg tccatgacgg tgcgggaacg ggtttgcttt
      961 ttgttagtct gtgccatcct ataagtagtg caattacggg tatcagaatt ccgtggctgt
     1021 attcatcgcg ctgccaggaa ttccagagtc ctccatagat tgggaggaag gtcagcacaa
     1081 ttatggttag ggatagtgcg cttagccccc agaaactaaa ctcagaccgc cagaatttcc
     1141 tgaattttcc agcaacgttt ttcaagtgcc cagaaccttt ttaatatgct tgcgcatttg
     1201 ggcggcgaag atgtctttcg cgaatttttc cgcctgcttt cggcaagcct ccggcgtgat
     1261 ggtctcttcc tttttctcga aagcttcgac ggcagcgatg atcgattccg gcgtttgttc
     1321 atcaaagaaa acgccggttt ccggcgttac gctatccagc gcaccgccct tgccataggc
     1381 gatgacgggc gttccgcacg ccagcgcttc gaccggcata atcccgaaat cctcctgcgc
     1441 cgcgaaaacg aaggcgcggg ctttttgcat atgctcttta agaaccggga acggctgata
     1501 acccaaatac tcgatattgg gagctttgtc cgcgatcgcc gcgaccttgt gggcttccgg
     1561 ccctgcgcca atcataacca gcttgcgctc cggcattttc gcaaaagcct gcgcaatcag
     1621 cgggaatttc ttgtaaggca ccatgcgcga tgcggtgaag taataatctt gcttgttcgc
     1681 acaggcttca aaatcctgca cggccaccgg aggatggatg accgccgcct cccgcccata
     1741 aaattttttg atacgttcgg cgataaagtt tgaattggcg atgtaggatg ttacgcgcgg
     1801 cgcggttttt ttatcccatt tgcggagtcg cctaaggaag aaacggatga gtttgccttt
     1861 cagccccgtc ccttgcccgg tcgtagagag atattcttcc tgcatgtccc aggcatagcg
     1921 aaccggcgta tgacaatagc aaatatgtgt ctgatcatcg cgcacgcgca cgcctttgat
     1981 tacgcagtga gaactggaaa taatcaggtc gtaggcgctt aaattcagtg cttctaccgc
     2041 catcgggaac agcggcaggt aatgacggaa ataacggcgc gccagcggca agtgctggat
     2101 gaacgtcgtt ttcgccctgc gcccgccgat aaaatcgcgt ttgtcgttgg gcagaaaatc
     2161 gaccagcgca tacaaatcag cttccgggta gagctttaat atctgctcca gaacccgctc
     2221 tgctccggca taggtttcca gccagtcgtg taaaacagcc actttcatgc gagttttata
     2281 cggatagagg gccggactgg caagataaga attcttgcac aggcttcagg ttctctttat
     2341 aaccggggct gatttcggaa ggcacaaatg gcaaagcatc cactctatat caatggccgg
     2401 tttcttcaga aacctgtgac aggaacggag cgttttgcgc gtgagatcgt ctgggcgatg
     2461 gatgagttgc tggcggagca gaccgatcgt ccggatgtta ctattatctc gccaccgggg
     2521 acgctcgcgc cggaagggct gaaagttatt ggctttgaga cctgcggatc gcggcagggt
     2581 catgcatggg agcaatggga tctgtatcga aaaacgcgtg acggcgtttt gctgagtttg
     2641 accaatagcg gtcccgttct tcacccgcgc cagatggtcg tgatccacga cggcgcgccg
     2701 taccggatgc cgggggattt ttcctctgct tatcggacgt ttcacaggat tttaggctgc
     2761 ctgctggtgc ggcagacccg gatcgccacc gtctctgaat tttcacgcag ggatctggct
     2821 gctgtgctgg gcgtttccgc tgaggctatt ccgtaattta caacgggcat gagcatatcc
     2881 tgcgctgcgc gctcgatgag gggatcattg aaaaacttgc cctgaagaac cggccatatt
     2941 ttctgtttgt ggggtcacca gtgccccgga aaaacctgga tatggcgacc cgggcatttc
     3001 atgcgctgga gcgtgaggac gcggcgtttg ttattgtcgg ggcgtcgagt gcgaaggtgt
     3061 tcgggaaaag gacgcagacg caagcgcaga atgttattct gccgggccgc cttacggacc
     3121 aggagattgt tgctctttac cgccatgcgc gggcgcttgt gtttcccagc ttgtatgaag
     3181 gcttcggcat tccgccgctg gaggcgatgg tgcaaggctg cccggtgctg gcttcgaata
     3241 ttccgccagt gcgcgaagtt tgcggggatg cggcgcttta tttcgatccg cgcgacccgg
     3301 cagattgcat gcggcgtatg cgggaatttc tggatgacct ttccgcgcgg gaggatttaa
     3361 tcagaaaagg cacagcgcgt tatccggcat tttcatggaa gacaagtgcg ggaaagatgc
     3421 tcgatgagct tatggatatg ggttaataca tttcttgcgt tatatagtca ttcaacttaa
     3481 gaattataca gagtcgaaaa tgactggctt ttgaaaaaat cgacgacaac gtacatacag
     3541 gtactttgag gagatttttt caaaaacagc ttcatttgca gacctgtata attcttaatt
     3601 caaacgacta tagtcctgcc tttttaatcg ctatttcata ttcttcgagt tgtttttccg
     3661 ggctgctggc ctcccagccg gcgcgggcga tggcggccat tttctctgga tcatcataca
     3721 tacgccgcat ggcgcgggcg gcaaaatccg tgtccgggtc ggcccaggtc acggtgccgt
     3781 aaacaccgct ttcatcgaac atttcaacaa ggcgcgcggg gaccagaatg ctatcgtttt
     3841 cattcataaa atccatattg ccggaccatg ctgttgcgat tgttgcaagc cccgccattc
     3901 gcgcttcaag taacggcagg ctaaaacctt cggcccgatg cagagaaaca aagacgtgtg
     3961 actttgttat cagctcgtcg atttcttcgc gcgagagggt tttcatcagg actttaatac
     4021 gcgagtcttt tgtcacagat tcaacaaaag ctttgatttc cggtatatcc gtgctgccct
     4081 gaagcttgat tatcagtgaa acgtcctgct tggttgggaa tgctttttgg aaagcgtcta
     4141 cagcggctgc cggattttta cgggtcaggc cggagcgcgc atccgccatt gtcagaaatg
     4201 tcagggactc ctgccctttt ttccatacgc gcggggccgg gctgtatttt tccatcacat
     4261 gggggacaac atggatagga catttataga gcttgccaag tccttgcgcg gaaaagctgc
     4321 ttgttgtcca gatttcgtcc cagacatcgt cataaaccgg ccagtttacc ggtggctccg
     4381 gcaattccca tgcgaaatag ccgatccttt tcccgctctc ctttaaactt tccctcaggg
     4441 gccaggcctg ttgcaaaaac tgtggcgctg cgcaatgaaa gatataaatg tcttgtttgc
     4501 tgccctcgat tttctttaaa gggtttctga ttgccggggt aatatcaatt cggcggacat
     4561 cgtagcccag cttttcaagg gcgcacatat gatagttggc tccgtttgta agcccgtttt
     4621 gcacacggaa acggcagaca atattgattg tttttttgct gtgtgtcttt tcatcttgtg
     4681 ctttactttt ccgcagggcg gtatgggcta tttttgcaaa gaaaaaatag taaatgcttt
     4741 tggctatttg tcgtaggttt tgcattgttc caatactcct cagcctaaaa cgagcgctcc
     4801 atataacgga taaaatgctt tatagacaag tttatatgat ccttttatgg tttccacagt
     4861 ccatatgccc tgacgtcatg aagagacagt tttatgaaat cttcttctgc cttgctaatt
     4921 tttattttaa tttttacctt ttttgcgttt gtggcgcagg cgcagcaaac aatctccggc
     4981 gttcctgcaa aaagcgcgga tgattttttg aactcccttg ggatcaatac ccacgttgct
     5041 caagggtata accccaaaaa ttatattgag cctcttaaat atacaggtat ccgtaatatc
     5101 cgcgatgcgc ctaaaaacct gtccggctat tttatgcttc ataaagaaac aggtgtacgt
     5161 gttaatcttg ttggcgccaa gctgcaccat ctgattcatg ctgcgcagac acttgcaaaa
     5221 cataatatcc ttatgtctat agaggggccg aacgagccga ataactggcc cattacttac
     5281 aagggtgaaa agggcggcgg caaaggttca tggaaggtcg tcgctgaatt tcaacgcgat
     5341 ctctataagg ctgttaaagc cgatccactc ctcaaggact atcctgtttt taatatttca
     5401 gaaggcggcg cgcaaactga caatgtcggg cttcagttcc tgactatccc cgaaggggcc
     5461 ggtacgctta tgcctgccgg gaccatgtat gcagattatg cgaatgctca taattatgtc
     5521 agtggtaacg gcggccgtca tgttgataat caggcatgga acgctgctga tccgacgctg
     5581 gatagtttct gggacgggct ctacggaaac tatgggcgta cctggcgcaa gaaatataaa
     5641 ggttacagca atgaagggct tatgcggttg ccgcgcgttt caacagaaac gggctgggca
     5701 actaaaaaag gcgtgtctga ggagcgtaca caagggatta tgcttgtgaa tacctacctg
     5761 gcgcaattca agcgcggctg gacttatacg ttcatttatc aactgggtga agctcaaggc
     5821 agtaaaggat tgcaggggat ttatcgcaaa gactggcagc ccaaagccgc ggcgcactac
     5881 atccataatc ttacgactgt tctggcagat acgatgtcag gtgattttaa acccggaagg
     5941 ctggcttata gaattcccaa tcaacccgat acggttcacg atctcttgct gcagaaaagt
     6001 gatgggaggt tttttcttgt tgtttggggt gaaagggtca agggtcagga tgatattatt
     6061 atccagcttg ccaaacctgc tgctaaaatt aaaatttttg atgtgacgga aggttctgaa
     6121 ccgcaaaaaa tgctttcaga tgttttagag attccgctta cgcttaatga ccatgcgttg
     6181 atattggagt ttcattaaat ctaaagaatc gttgctttgt gacaaagcta tgattgttcc
     6241 aaacaagatg ccttgaagca cgccgaaatc aggggttgtt cctgcaagaa attttgcagc
     6301 aaacatgggc aatagagcaa atttgatcgc cttgataacg gcctcttcac gtgaattgcg
     6361 ctttggtgca tggcggagat agagcgagat gaggaacagg ctgaaaaaga gtgcaccggg
     6421 aaggcccgta ctggccagaa tattcgcaaa gaagtttgac gttcggacac tcccgatacc
     6481 aacgcccagg ccatagcttt caataaaagc attaagccct gccgccgtcc agcttgagcg
     6541 ttctatatag gaagatgtcc ctgttttttc gattaaaagc gtattgatca gatcaacaaa
     6601 atttccactt agtaatacag ctaataaaaa acagacggca agaaaaacga tgttcagcat
     6661 tttttttgtg atagatttat gcaagatctt tccttcttca gaaacggcat caacattata
     6721 aaaaagatag aggccgagca tcaggaaaag agaggcgtag gccgttgaag aggtggataa
     6781 gaagcccatg gccatacagc tccagccgag gagcggcacc caaaaacggc gcattttttc
     6841 cgcatagacc gggcgcagga aaagcagcaa agaggccatt gtcatggcaa gtcctccaaa
     6901 agctgcgggt tcaggcgtca ggcccagcac acggcgcgtt cccaaaatac tgcctgcgga
     6961 atagccatag ttggccgtat agaaaatttt gagcatatcg gcctttccta aaaggttggc
     7021 gataatatca ataaggcctg ttgcgatgat cgcatagcca cctgcgaaga ttgcctttaa
     7081 cagcgcatct agaaattcat gtttcttttg taaaagaatg caaacagatg ccgccataaa
     7141 aacggacatc gtcaggtagg ctgtttgtgt atagttcgtt ccttgtggac gaacaggttc
     7201 caactctgcc gtgtttaagc taaaagtaaa aatctggccg ttaaataatc ggggcagaaa
     7261 catcgctccg ataagggaaa tcagccagaa gattcccaaa aacccaagct gacgcggttg
     7321 aaaaagcgca gcaaggaaag acggcacaaa attctgactg atgccgatac gaaacaagaa
     7381 tataatggat gcgagcatcg cggggacaac gtttcccggc aacacggtca ttgcactaaa
     7441 ggaagagcat atgaagacgg atgctaacag gatcgttggt gaaagaataa tacttccaag
     7501 aataatgagc agcgctatga cagaaaccgt attcatttca tgatgctcct agcccagccc
     7561 taaccagcgc tgcaggttaa atctccgggc catatcataa aaaataacag ggatgattgt
     7621 tcccagtatt aaagctatag ggaaaatgac aagcggatcg gaataaccaa gacctttcac
     7681 cataaaaatt cgggtgccgg ctacaatcat cacgtggaga acataaatag gcatggtcct
     7741 ttggccaata tacactaggg cagagaatga ccgaatgtag gatatgcgta ccaggaaaaa
     7801 gataaaggcg caccccagga tagtggatgg tacttggctg agatattttg gcgtaccgat
     7861 ttctgcaaga tttgtttgag taaagctata tgaagaaaca acaaaccaga ggatgcctgc
     7921 gacaagtgtt aaaaacagga atgaaaggtc tgtccattct tttttttctt tatcctgaaa
     7981 caaaagcgtt ccaagtccat aatatatccc gaacttagca aagggggtaa aaaacgaagg
     8041 aagggccgga aacgtagaaa gacctaaaag aagcaagaaa aagaccaatt cgtatttttt
     8101 attcataaat ctggaaaaga ggtaatgcac gatctgcatt aaaatcagag cttttaaaaa
     8161 ccagaactgt gaaacatttc ctagcgctat atttctgatc tgaaaccatg agaaatcctg
     8221 ggggtggttg aggagcgagc cggtcagatt catggaaaat gtctggaccg cagaccatag
     8281 cacacaggga tagtaaatcc tgatcagaag gtcttttgca aatttctttc cgtccttctt
     8341 ttccaaagac gaggcaaaca tccccgataa aaagaaaaag gcaggcatgt ggaacgtata
     8401 aatataattg aaaatttgca ccagaattcc atcgatcggg acaagctgag catctcgtaa
     8461 gccgccgatg acatggccga taacaacaag catgatgcat aggcctcgcg ccgcatctgg
     8521 ccagtctata cgctgggatg cgttatgtac attagatgga tagacctgtt cggacttata
     8581 agacactatt tttccttggg gaggttaagt ccaggcttgt tattatgatt aaaaataatt
     8641 ttgtcattta ttgcctggct ccagacttta aagtcttcag agaaataacg ttcgatgggt
     8701 tttgtaggta atgtatcatg tttttgagtg gttgatttgt tattgtgtgg aatttctcct
     8761 gtagccttgt atcgcttacg caagaactcg attccttttt tcatatcttc aataggaaac
     8821 aatagatcga caataatatc gccatttgta tcgcatataa aatccgtttg tttccgaaaa
     8881 tggctccaac gcataatttc ctgttcttcg atatgttcaa tatcgttcaa ccaatataca
     8941 aaatcttcaa atgtttttaa gaatgaatat tcttcctcca ttaacttttt ttcagcaggc
     9001 actttcgtgt atgagcgata ataagtaaag gctgatgcaa tgcggtcaag cggatttctt
     9061 agcggtgcaa ataccagcca tttactgtaa taattaggga aaagtgtctt ataaaattct
     9121 gcggaataat gcggaagctc atactttgtt tcgggaaata aaaattgaga tagtgacgtg
     9181 gatgcatttt ttggtatgcg aataaagaag gcttctgctt tggaaaaatg ggtaataggg
     9241 ttatcttgag cgtgtaattt gaatttaagt ttagttttta gaatttttac attttgcgaa
     9301 taggggatgc gctttaaata tttattgttt acttcaagac cgggaggata aaaaactgac
     9361 atcttaaaaa ctcaatttaa aaactcaatt tgaataagtt gttgagaagt attgtatttt
     9421 cctaaaaata aggaatctgc ttttaagccc ctctcttaat aatctggtga acattgcatt
     9481 ttccctacaa aatatcaaac aaatccatcg aattgtccgg cagggaatct tctccttgct
     9541 attttattac ttacaataca tattaccgct tgatccgaga ctggaacagt tactctatat
     9601 ggcggctatg aatactctga cacacttaca gcggctggaa gccgaaagca ttcacatcat
     9661 gcgtgaggtt gtggctgagt gcagaaatcc tgtgatgctt tattcgatcg ggaaggattc
     9721 ggcggttatg ctgcatctgg cgcgtaaggc gtttcatccg tcaaagctgc cgtttccgtt
     9781 ggctcatgtc gatacgacat ggaaattccg cgatatgatt acctttcgcg ataaaatggc
     9841 ccaggaagct ggcgccgagc ttttggtcca tattaacgag gaaggcgtca aacaggggat
     9901 tgggccattt acgcatggtt cggcgctgca tacggagatt atgaagacgc aggcacttaa
     9961 acagatgctt gatcactatc aattcgatgc ggcatttggt ggtgcacgtc gggatgagga
    10021 aaaatcccgg gccaaagagc gcattttttc atttcgttca aaggcgcatc gctgggaccc
    10081 taaaaaccag cggcctgaat tgtggaacct gtataattgc cgcaagcacg atggagagtc
    10141 gatccgggtt ttcccgctat ccaactggac agagttggat gtctggcaat atatctatct
    10201 ggaaaatatc ccggtcgtac cactttattt tgccgccgag cggcccgtgg ttgagcgtga
    10261 cggtacgttg atcatggttg atgacgaccg gatgaagctg gaaccgggcg aaaaacctgc
    10321 catgaagaag gttcgttttc gtacacttgg ctgctggccg ctgaccggtg caattgaaag
    10381 cagcgccgat acgctgcctg cgattattcg ggaaatgctg ctgaccaaaa cttctgaacg
    10441 gcaggggcgg gtgatcgaca atgacagcaa tgcttctatg gagcagaaaa aacaggatgg
    10501 ctatttctga tgcatagcga ttcagccctg attgaagagg atgtggaaga gtatcttcgg
    10561 gcgcatgaac acaagacgct gctcagattt attacctgcg gttcggtgga tgacgggaaa
    10621 tcgaccctga tcgggcggct cttatacgat tccaagatga ttttcgaaga ccagctggcg
    10681 gcgctggaaa gcgattcaaa aaaggttggt acgcaaggta attctattga ttttgcgttg
    10741 cttgtggacg ggctggcggc tgagcgggag caggggatca cgattgacgt ggcctaccgg
    10801 tttttttcga ctgaaaaccg taaatttatt gtggccgata cgccggggca tgaacaatat
    10861 acccgcaata tggcaacggc cgcatctacg gcagatgtgg ccattatcct gattgatgcg
    10921 cgcaagggcg ttttgatgca gacgcggcgt cattcgttta ttgccagcct gatgggcatt
    10981 aaacatgttg tcgtcgcggt gaacaagatg gatctgattg gctatgacga agcagcgttc
    11041 acggcgatta aagaagatta ccgcgctttc gccaaggggc tggatattga gcatattacc
    11101 tgcattccga tgtcagccct gggtggagat aatgttatgc agcgcagcgc taaaatggat
    11161 tggtatgatg gcccgaccct gatggcctat ctggaaacag ttgaggttga agatttagcg
    11221 caggactgtc cgtttcgtat gccggttcag tgggttaacc gtccgaatct tgattttcgc
    11281 gggttttccg gtcaggttgc cgccggccgg attgagccgg ggatggaaat caaggttatg
    11341 ccgtctggca aaacgtcccg tgtcagcaag atcgttacgt tcgatggtga gctgcccgag
    11401 gcggtggcca gccaatccgt aacagtgacg cttgcagacg agatcgacat ctcgcgcggc
    11461 gacatgattt gtgctgccga acatccggcg gaaattactg accagtttga agcgtatctg
    11521 atctggatgg gagatgaaaa aatgctgccc ggccgtcctt atatcatgaa aaccggggca
    11581 catcaggtgc cggtaagtgt gacgaagctt aaatacacga tcaatgttaa cacgatggag
    11641 gagcatgctg cccactcttt ggtgcttaac gaaatcggcg tgtgcaactt ttctgccgaa
    11701 cgggattttc ctttcgatat attccgggat attccgggga cgggcagctt tatcctgatc
    11761 gaccggatca ccaatgctac ggttggcgca gggacgatta acttcgcgtt gcgccgggcg
    11821 tctaatattc actggcaggc tcttgatgtt gacaaaaata cacgcgcggc tctcatgcat
    11881 cagacgccac gggttgtttg gatgactggt ttgtcggggg ctggaaaatc gacgattgcg
    11941 aacctggtcg aaaagaaact tcattcactt ggtaagcaca cttatacgct ggatggggac
    12001 aatgtgcgcc atggtctgaa ccgcgatctg ggctttaccg atgctcaacg cgtcgagaat
    12061 attcgccgcg tggccgaggt tgcaaagctg atgaccgatg cgggattgat cgtgctggtg
    12121 tcgtttatct cgccttttcg ggctgaacgt cgtatggcgc ggcgtttgtt tgaagatggc
    12181 gagtttatcg aggtttttgt tgatgcttct cttgaaacgg ttgaaagccg tgatcctaaa
    12241 ggtctttaca aaaaagcccg caaaggggaa atcgccaact ttaccgggat cgacagccct
    12301 tacgagcgtc cggaatacgc ggatctgact atcgattcaa acaagatgtc cgcagaagaa
    12361 gcggcagaaa aaatcgtggc tctgctgatg gatacgtaga ggttgtcgtt acgtattata
    12421 tagctgcgcc aattttaaac tgtcttcaaa ggctttataa attttttctt tatgcgaggg
    12481 tttaactgta tcaatagaag gaacttcttc agattgtttt ctgaacccgc ttttggcttt
    12541 tgaagtcata ctaaaaccca tcaagccctt tgatgagaaa aagccttgta gtgatttttc
    12601 caaattctca tatggcagaa cgagatctac atcaagttcc aaatcacctg tttcattgcg
    12661 tttgctatat agtgaaacat cttctgggaa gtctcctttt tctacatatt cctcccatga
    12721 tagtttgtag gaactgtctt tgttgtgata ggaagaatat cgaagcatac agtagtgaga
    12781 gatgcattta tcgatgggtt ctctttcaat acaaaaaaat gttagctcca ttgacttttc
    12841 tttgccaagg aaatttttta tttcggaaga agacatatga ttgtaaattt tttgtttaaa
    12901 tagtcttttg tggtttctgg ctttgtgtcc ttcttccttt ggtttaattt cagtcaggat
    12961 gtcggattga ggcagcaacg gcgataggga aacttctata gcagttcctg ctgttttatg
    13021 agttttaacg aatgcaaaat tgtgtttgaa acttatgaca gccatactaa ccttctgctt
    13081 gagctgatgc agagtaatca tcagccattc tctttttgta gtgactatag cacccttatt
    13141 gctgcgttaa aaataaacat acttaatttc aaaattcctt ctgtgtaatc cggcgtggat
    13201 cacatcttcc gcgcgagaga tcaaacaaag ccatcagatt gccgcgcaga cgccctgtat
    13261 aatcgacatc gcggcggatg gggataaggg ttttcaggat attggcgata aaattgcgta
    13321 cggcttgcat aaacccggcc ctgaacggca cagtgccttt gcggatcagg taatggaggt
    13381 ttgcgatttg cgaatagccg aaacgcaggc cgctggtgcg cccgccagtt gcgcccatat
    13441 gaacgccgat acaatccgga taacggatta cgcggcctaa tcgtaaacat tggccggtaa
    13501 aatcgcgatc ttcctgccag ctataaagcg ggaggttttc atcaaagcgc agagcgctga
    13561 tagccgtgcc aagaaacgcc atattgcagc cataggcgca gtctgtttcg ctttcatggt
    13621 tctggttggc ccagtgttta aggggaggtt tttgctcact caggaaagcg gcggcttctt
    13681 cttcgctata acctccgctc atcacgccgt cagccagaac gcgacccgtc aggccgacaa
    13741 tatccccttt ggcgaaacgg gttgcggctt tttccagcca gtcgggcgcg ggccggaaat
    13801 catcgtcgaa aaatgtgcag aagtaactat tcggttcgcc gttaaaagcg ccttgggttt
    13861 ttaatgcttc aattcctgcg ttgcgctgac aggttaatcc gatttttgtg gaaacaaaca
    13921 cttcgtaaga tcctgaaagc gccatagggt gatcgttcaa tccggcaata tcctgatctt
    13981 ccgctccgac gacaagagtc atcgtggggg taagcgtttg ccgcgccaga taatccagaa
    14041 ggacgtatgt ttccttcgcc cgtccttttg tggcaataat tacgtaggga tatatcatga
    14101 ctctaactgt tttatcttat aactcaaaaa caaatatgtt aaattctggt tggacagcag
    14161 cgacaaaata atatagaaac gctccattag gcaagagaac tcttttgagc gcgccagatg
    14221 tcatgagctc ttatgcgttg tccctgttaa tttaaggtct atgttatgac agcaaacaat
    14281 aaccgtccta tccacaatct cgtaaaggac tgtttgggat tcgctgtgag taacataaac
    14341 ggccaatgtg agtttagtaa atctatcttc gttattgcac atatgaggtg cggctcaact
    14401 gcgcttagta acgtgcttac aaatcaccct tctataagtg gttttggaga ggctcatata
    14461 gagtatggaa gtcggtcaaa acttggaaat ttagttatac ggcagataaa agaaaaaagc
    14521 tggaaacata aatccgacta tttatttgat aaaatattgc actcgtctta cgacaaaaat
    14581 gtttgtgaag acttttataa ttcaaaagcg atttttttgg caagagagcc tgaagcttcc
    14641 atattgtcca ttcgcaattt gtttgaacga attaattcca aagatttttg tacagaccaa
    14701 ctggctgcgg attactatat agaacgcgta agcgacatta taaaaacctg gcatcgtttt
    14761 gaagccagga acaggcttgg gctaacgcat agtgatttgg tttccggtcc cgatgcgcag
    14821 cttaatagaa ttagtgcttt cattggaatt tctcccccgc tggaaaacaa atacgaaagt
    14881 cgcagcgctt ctacaaaaga aggagcgggg gaccctcttt cttctgacaa gtttaataag
    14941 attctagccg acggtgacgg aacgtcgctc tcaaaaacaa gaaaccttga aattacggaa
    15001 tcaacacacg aaaaaataca aagcgtctat aagaagttta tacgtataat ccgtgatgat
    15061 cctgtgtaga tgttttaatt ggacagcggt ggcaaagtaa tataggaacg ctcgtttagg
    15121 taaggcaact ccaacacgcc taacataatt tgctcttacg ccttgtgttt ggagcgcctg
    15181 aggttatcgg atcatgtctt ttgactcaaa agaaatacct ttttcccaca aaattgcgaa
    15241 tggcgggtct ctgaccatcg cgggaaaact tgtgtgccgc tgtctggacc ttattactct
    15301 ggctattctt tcgcgtctgc tcagcccggc tgactttggc ctgaccgcgc tggctatgac
    15361 atcggttatt attgcagaaa ctgtgacgtc tcttcctttg gctgctgctt tgttacgtgt
    15421 ttcaaaacca agccgggcta tgtatgacac ggcttttacc cttgcctttt tacgcggtgt
    15481 tttgttggca gtcgttttga gcgcattggg atttgtcatg atgtatattt ataatgaacc
    15541 ccggctggtg gctttggtgg cagccttgtc tatcgctccc attttgcggg gtcttcttag
    15601 tccccgcatg gtcgattata tgcgggagat gaattttatg ccggaagccg gcattgaaat
    15661 tgcggggaaa accgctgcac ttgttgcttc ctgtgcggtg gctttttcca cccattctta
    15721 ctgggcgatt gccgtttcaa ctattacaac gccttttgtc atgaatttgc tttcttatcg
    15781 ggtcgcccct tacaggcccc gtttttgcct atcggaatgg gtgttgttta aaaattatgt
    15841 ggggtggaat tcgttgacac agcttattgg tgcattgaaa tggcaaatgg ataagctctt
    15901 gcttggatat tttgtaccca aggcaacgct tggtcagtat acgatggcgt ctgatcttaa
    15961 caatattccg cttcagtcta ttgttgtgcc tttgaaacgt ccggtgcttg cgtcttactc
    16021 agcacgtggt gtaaggccac cggaagaatt gatgaagacc tatcttaaga catcaaatgc
    16081 catgctggct tttgccgggc cggtttttgt tgggctcgcg cttttggctg tgcccgctgt
    16141 atatctggtt ttaggtccaa aatgggaaga ggcaggaagc cttttgcaat ggattgcgat
    16201 tgcagcattg ctgactctgc ccgtcattaa ccttgatcag ttggccttag cgcttgatga
    16261 agcgcgtatg ctgacatggc gcacggtttt agaattttgc gtcgctgtgc cgttgttgtt
    16321 ttttgggtgc gttacttatg gcgtgatggg ggttgtcggc gctcgcatta tcggtgggtt
    16381 gtttacggta cttatcagta tggaggcggt gcgtaagctg attgggtgtt cttttgcctg
    16441 tcaaattatg gtattgcaac gcactgtatt tgctcttttc cttatgggtg gaagtttgtg
    16501 gtttttacgg cctctggtta ttatggacga ttctgtgatt gctttggcct ttagccttgc
    16561 cggtgtgggg ttgggggggc tgttatttta catagtatcc attgctattt tttggcgact
    16621 ggctggctat cctgccggta ttgaagctgt cgtgtgggga aagggagaaa atatactttc
    16681 acaaatgaaa aagcggttgg tttcctgatg tttttcagga tttaatttcg tctaaaatct
    16741 cccggtaaag cgttaggctg tcctcggcgg cgccagtctg tgtacctcgg ccataggcat
    16801 cgacgtagcg gataatgtct tcttcgcggc agatgcctgt ttgtttttcg tagatcttac
    16861 aaggcttgct tgtcaggttt gccatgcagt gtacgctttg aagcgggata ttgatcatat
    16921 cacctgcgtt cagagtcaaa cgctgtccgt ttagcacgac cgtcagcgta ccttcttcta
    16981 cccgccatag ttctgtacga agctcatggg attgtaaaga aaggattttt cccggcataa
    17041 gcgtgatggt cttctcgcaa tattcgctgc cgtctttgag ggggccaaca ccggttacaa
    17101 cgtagctgcc ccatgggcgg gtgtcgctgt cgccaatctt ataggcttca agatcgcatg
    17161 catatctttg cactctgttt atggcggtat tcagtgtcat gactatttta ttatttttgc
    17221 ctttattcag gcttgaatgc cattgtattt tttcattatc tcctgttaaa agttatgtca
    17281 tctaaaatca agttttttct ttacggtggg tagacttgct tattttacag gcttctaact
    17341 ctttgaaaat tccttatctt ccggcatcgt cacgcctttc ggcagatggt tccgccattt
    17401 agcaaaagcc ggtttattta tgggactagc gatgtagaag gtaccgtccg ggctttcttc
    17461 tattttcgcc catgtgagtg tacgcgcctt atccggttca tcctcacctg ttttagcgtt
    17521 ttttccgatt aattgtttaa cgccgtcttt ttcttcgatc gtataacctt gtgcggtcca
    17581 ccacgcggct gcaatttcat taacaacagc caacgcattt agaggtggtc ccaagaattc
    17641 ggacagtgag ttaagctacg atctatgacc gaa
//
LOCUS       DAOSWR010000030        17440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2065_length_17440_cov_4.882888, whole genome shotgun sequence.
ACCESSION   DAOSWR010000030 DAOSWR010000000
VERSION     DAOSWR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17440
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_17440_cov_4.882888"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..537)
                     /gene="truA"
                     /locus_tag="PK513_03700"
                     /pseudo
     CDS             complement(1..537)
                     /gene="truA"
                     /locus_tag="PK513_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
     gene            complement(1008..1706)
                     /gene="ftsE"
                     /locus_tag="PK513_03705"
     CDS             complement(1008..1706)
                     /gene="ftsE"
                     /locus_tag="PK513_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOO81588.1"
                     /translation="MIKFEHVGLRYGVGPEVLSDINFTLEPGSFHFLTGPSGAGKTSL
                     MTLMYLGRKPTRGLVSMFGQNINDMSRAQLGLLRQKIGVVFQEFRLLPNLSAFDNVAL
                     PMRITGHKESEVKSNVDELLEWVGLGDYKNALPLTLSGGQQQRVAIARAVIARPKLLL
                     ADEPTGNLDDDIGFRLMHLFEQLNRMGTTIVIATHSQSIMERFGHPRLVLEDGRLYMH
                     DNANWVQKNIAGVY"
     gene            complement(1724..2467)
                     /locus_tag="PK513_03710"
     CDS             complement(1724..2467)
                     /locus_tag="PK513_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81589.1"
                     /translation="MTGRIFLFVGILTLSLFPYAAGALRAAGCLAQDRACILKEIETE
                     SAQIENTSWRDQTYRELAKTKAADGDMDGAIALIDKIETPDTQAMTIRGIGMAAADLK
                     LEKAAYDALFTKLRTRAEKIEHPPSYAIALTYIAMAQAFAGDNEGAWKTASEMENDAL
                     RHKAYGETAEIQAEVGNAHAAMKSIGFIDSIAFRNKAYAIVSKILADRKDFDGAMAAA
                     MTITNPYKKAKALQYILDVQKPREVEKKY"
     gene            2684..3406
                     /locus_tag="PK513_03715"
     CDS             2684..3406
                     /locus_tag="PK513_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81590.1"
                     /translation="MPDPDELIENLEAKIEGIPESVKPIPEGSSVPALANMPEEREET
                     RSAIMAGYVGALLVFVVILAALLVMKQTLVKTWPPGAALYEFMGIEVKSPGEGLVFDR
                     VKAKVTPTGRIIIEGSIINLINEDQILPAIKVSVRDQAGQEIKQLTIQPPFEQMKAES
                     TLPFKTSYKDGLNGADHIQLRFVLGDPLQEKTVELKGFEEEHAAEIEGSHEEGKHGEH
                     TSEHTLPEHTPQEHNPAPESHH"
     gene            complement(3410..5896)
                     /locus_tag="PK513_03720"
     CDS             complement(3410..5896)
                     /locus_tag="PK513_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HOO81591.1"
                     /translation="MSANPEFLRNKGVNLAWLHFISRAALMIERLGMALWRLASWCMG
                     FGALWLFEIPQGLGAVVSAITTLIFFIGLVHFIRKDIRSFRWPKRTEVQRRIEQVSGV
                     HHRPLSTLGDRPVQAGVNALWAWEQSRKMQMLAKLRPALPRALLAARDLRALRLGLLI
                     ALICGVIVAGADGKRRILSGLWPVGGLEIFERQAPEIAFWITPPEYTTQPRIFPPETD
                     GVLHIPQGSVAKAIATRSLLHALRMPALLVDGKAYPFESGEDGASTLEMEIPVGHKLR
                     LRTGLWGGPVWDYEFIEDLPPSIGITEDPHALENGQVQFALTMLDDYGVKTLHMRMTL
                     DPSVDKAPMGWAAQEERSVMSPAKTEFQMAPVYDLAAHPWAGLPVVFTFSAHDHLDQI
                     AQTLPLRLQLPERSFSHPVARQLIEMRKYLIWNPDQPYGEVGLALEKLLYQPHTYGHD
                     LRVFLALRVAASRLYYNEPSEMVARSVIGLLWDIALRLEDGNLSLAARDLRELQKKLE
                     QALQNPEVSDAEIAILMERLRQAMAEYIQALAREWQKSVQREEMQPTQLPPEVLAQMI
                     NMNDLAGLMDQMEQDMLSGDRTSAQKMLSKLQRLMDMAGPSKNFTMPPDMQMMQKAIK
                     DLQELIERQESLREQTAKQAELYEKLEGLGVDGSFEDQPPPFINTVVQHTEQEALYMT
                     LVQLMKEAETALGEIPENFELAAQAMDSSGEQLGLSRPDLSLPFQDEAIKQLKESQQQ
                     MAQMMAQWMQQMTGLAFGGMPMQLDPLGRPYGGHDGKNGLPFGDQVKIPSEAERKWVQ
                     EILNELRRRASDRRRPQEELDYYRRLLKRF"
     gene            complement(5936..7207)
                     /gene="lysA"
                     /locus_tag="PK513_03725"
     CDS             complement(5936..7207)
                     /gene="lysA"
                     /locus_tag="PK513_03725"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOO81592.1"
                     /translation="MSGFHEQNGVLCLDSVPLSDIAKKYGTPAYVYSARVIRDQYDAL
                     AGALQKTLPANRQPLLCYACKANSNLGILKLLQSLGSSIEVVSGGELKRALKAGFAPE
                     KIVMEGVGKTEEEILAGLRARVHQFNIESFGELDLINRLAGQEKQLADVVFRLNPDVG
                     GAAYKKISTGSKGDKFGLAPERVLEGFARAQSMEHVRALGIFTHIGSQITETGSFEAL
                     FKKIADFVGLLRSKEYEVSRLDIGGGFPIQYQDEDLLDLDSYANAVREHILPLDTEII
                     MEPGRYLVGNAGVLLTKVLYEKESYGEDFLVVDGAMNDLMRPALYGAWHGIEPVANRD
                     AALHPYNIVGPVCESSDVFAKQRNLPEMKPGDLAVIRTAGAYGVCMASNYNTRPLPPE
                     ILVDGDKIALIRARQSYEDLLGADIIPDWLV"
     gene            7569..8867
                     /locus_tag="PK513_03730"
     CDS             7569..8867
                     /locus_tag="PK513_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOO81593.1"
                     /translation="MQEQNAQSPTLESISQDQLDALVKLHNRFLDGRLGGRRALLKNT
                     DLTGLSLRAQDLRQASFMGCIMVGMDLSRANFQEASLYACDLSNSNLNDTRFVRADLR
                     GARIENATLQGADLEKADLRVGGLSDGQSYDSGQAVNFRGANLSGAKLAGSIASKANF
                     SDAIMTGANMSKADLRGAQMEGADLSGADIDGAQLSGANFKSAILTGVEVKKIRETLD
                     IDLSDAITDENIGASVADLDEPLAKLIEDHRGWVETAGAKGRQLDLSDMDMRSLRTLK
                     MERMTAIRAIGTKFLGMNLYKIELQSAVCDGADFRNCDMEEADLRGSSFAGANFSHTK
                     MKGVDGSPLMFGAEGVSKRFSPCNFENAKLRYVDLSGAQLKSAVFKGADLSYADLSGA
                     DLRESDFTDANVTGTNFDGAQTEGANIPKADKPVFQMKRD"
     gene            8970..9506
                     /locus_tag="PK513_03735"
     CDS             8970..9506
                     /locus_tag="PK513_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81594.1"
                     /translation="MTVNYEKAENKAGDNDAEAVVETVTEVAAGAVTVSVPAGGALVS
                     AFKDVDNLPLNLIKKMGSKRLPWLLGTAATAGFVVDEVEAHWEKGDVCKAFGAALAGI
                     AETGGNFVPGGNYLLLGDTFHKTVRTAYIGVTEGVLGFEEGQCTPNETDIVAAVDYLS
                     SDGAPRMVMNINDEKLAP"
     gene            complement(9517..10446)
                     /locus_tag="PK513_03740"
     CDS             complement(9517..10446)
                     /locus_tag="PK513_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO81595.1"
                     /translation="MTILVLAKHDNAELKPATLSAIGAAQKIGGEIHVLVAGANCASV
                     AEAVAKAEGVSKVLKADAPELEYGLAENVAPLIVELASGYSHIIGASGTYAKNILPRV
                     AALLDVQQISDISAVIDADTFERPVYAGNALATVKSSDAIKVITARGTDFDAVSPEGG
                     SAAIEDIAAKGDAGLSSFVNLEVSKSDRPELTSANIVVSGGRGVGSKEDFALIEKLAD
                     KLGAAVGASRAAVDAGYVPNDYQVGQTGKVVAPALYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEDAPIFSVADYGLVADLFDAVPELSEKL"
     gene            complement(10448..11212)
                     /locus_tag="PK513_03745"
     CDS             complement(10448..11212)
                     /locus_tag="PK513_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO81596.1"
                     /translation="MKILVPIKRVVDYNVKVRVKADGSGVDLANVKMSVNPFDEIAIE
                     EAVRLKEAGKADEIVVVSIGPEKAQEQLRTAMAIGADRGILVQTDAPIDLGGVEPLAV
                     AKMLKALVEKENPDLVIMGKQSIDDDSNQTGQMLAALLGWAQGTFASEVTLDEDSLNV
                     TREVDGGLQTVALKMPAVVTTDLRLNEPRYAKLPDIMKAKKKPLDVTNPDELGVDITP
                     RLEVVKVAEPPVRQGGGKVSSVDELIDKLKNEAKVI"
     gene            complement(11240..11440)
                     /locus_tag="PK513_03750"
     CDS             complement(11240..11440)
                     /locus_tag="PK513_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HOO81597.1"
                     /translation="MNGLLATSIVFAMLCVLAVLAIGLFAMIKGGEFNKKYGNRLMQA
                     RVVLQGVALSLLALAYYASQSS"
     gene            11568..11786
                     /locus_tag="PK513_03755"
     CDS             11568..11786
                     /locus_tag="PK513_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81598.1"
                     /translation="MQSKHHENWTYKWSNTSMGNSFAMFNCPEIGTNILYTFEQIKEI
                     LLNNKTTNIPYSVLAEAANVYLGVKSVA"
     gene            complement(11793..12518)
                     /gene="ubiG"
                     /locus_tag="PK513_03760"
     CDS             complement(11793..12518)
                     /gene="ubiG"
                     /locus_tag="PK513_03760"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HOO81599.1"
                     /translation="MSSIDTQEIENFSKDAEQWWDENGPFKPLHRLNPVRMRYIKSQI
                     CRHYGRDVESLRPFEGLSFLDVGCGGGLVCEPLARMGGTVSGADADAVAIETAKRHAQ
                     TSGLKIAYLNKPAEAIEQKFDVVMALEIIEHVTSPETFVQNVANLVKPGGIVIFSTLN
                     RNPKSFLMGIVTAEYLLRWVPRETHSWKKFLRPSELVKMVEKAGLEACNLTGLVFNPF
                     KNEFELSESDLDVNYLMSTQKAV"
     gene            12577..13809
                     /locus_tag="PK513_03765"
     CDS             12577..13809
                     /locus_tag="PK513_03765"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO81600.1"
                     /translation="MALIVAKFGGTSVADVERIQRAAGKIAREVEAGHQVVVVVSAMS
                     GVTNQLVGYCNDISELHDLYEYDVVVSSGEQVTAGLMAMALHKHGITARSWLGWQIPF
                     KTDEMHSKARVRDIETAGLLEGLKKGEVAVVPGFQGITAAGRISTLGRGGSDTSAVAL
                     AAALNADRCDIYTDVDGIYTTDPRIVSSARKLKKISFEEMLELASLGSKVLQTRSVEI
                     AMKHGVSLQVLSSFEDQIGSDMPGTLVVNEEKNMEEELVSGIACKRDEAKITLLRVAD
                     VPGIAAKVFSPLGRAGVNVDMIVQNISADGKATDMTFTVSRTDLPRALEALKDIDIEY
                     EEIRSSGNVAKISIVGVGMISHPGVAEKMFTTLAEKNINIHVISTSEIKISVLIEEDY
                     AELAVRALHNAYGLEEAA"
     gene            13828..14655
                     /locus_tag="PK513_03770"
     CDS             13828..14655
                     /locus_tag="PK513_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOO81601.1"
                     /translation="MMANPATELQDTTFFTDMPVGEILRRTRTHYKRSIEDIEHALRI
                     RTEQIEAIEQGNLEMLPGRVYAIGFVRSYAEYLGLDGDKMVDLYKQQVMAAKGPEPEL
                     YFPIPASDNHLPPWWITLSSIFLAIALLAFWFGENRAGRKAVETIPPVPEEIQIAVEE
                     PTPPVASKVEIEEPEEAVIEDKPVEGIILKIHENSWVEIRDKKGKAMLSRVLKAGDQY
                     YVPDRPDLTISIGNAGGVEIEVDGQPLRKLGEKGKVLRRLSLDADYLKSNFAVERAQ"
     gene            14675..15739
                     /gene="prfA"
                     /locus_tag="PK513_03775"
     CDS             14675..15739
                     /gene="prfA"
                     /locus_tag="PK513_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOO81602.1"
                     /translation="MSLEEKLVSIKNRHAELAALMSEGGLSGEKFAKLSMEYAEIDPV
                     VEAITTLAEAKAERKDLQEMLADPEMKNMAAEEICALDAKMPKLEHQIRLALIPKDED
                     DTKNAIVEIRAGTGGDEAALFVGDLFAMIKGYAIKQGWKLEVIETSQSDAGGYKEIVC
                     EISGHNVFSKLKYESGTHRVQRVPKIESQGRIHTSAATIAVLPEAEEAEVNIHSDDLR
                     IDTYRSQGAGGQHVNTTDSAVRITHIPTGVVVEMQEERSQHKNKAKAMKVLSTRIYDI
                     QRQKLHDERAQDRKSQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLEEVLSGETLS
                     EITEALIAEDQASALASVEN"
     gene            15766..16620
                     /gene="prmC"
                     /locus_tag="PK513_03780"
     CDS             15766..16620
                     /gene="prmC"
                     /locus_tag="PK513_03780"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOO81603.1"
                     /translation="MKLNILYKTLRQSLSESDAHYVIQKRTGLNRADMISNPDQIVDK
                     LQILKDLQRHQNGEPLSRIYEEREFWSLSFSLSPDTLDPRFDTETLIEAVLARYKSAP
                     PKRILDLGTGTGCILIALLSEFPEATGVGVDLSEGALKTARSNAEANKVENRAAFIQS
                     NWAECIDESFDLVVSNPPYVSPKAIQNLDKSVQNYDPILALHGGADGLQAYKEIFSDL
                     FRLLNPGARAFFEIGFDQHDSVMRLGEESRFLVEGLYLDLAGHIRVLELTPQNSDGDK
                     VKNNLPDG"
     gene            16807..17217
                     /locus_tag="PK513_03785"
     CDS             16807..17217
                     /locus_tag="PK513_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HOO81604.1"
                     /translation="MRHGTSNRRQRGRGNGQRRGNNQRMQVYDSNGPDVRIRGTAHQV
                     TEKYTALAKDAASGGDRILAESYLQYAEHYQRIINSWEEGVQDDKLRTSETQENSQKK
                     SKVSDNDDDLSLPSSILGEKHNIKSEEYATEASA"
BASE COUNT         4599 a         4599 c         4075 g         4167 t
ORIGIN      
        1 cccgatcgcc agcaacaccc caacgatatt gcgcaccata tgatgcaaaa aggcactggc
       61 gcagaagtcg aaaacgatga gatcgccctg gcaagtcaca tcagcccgat gcaacgtctt
      121 cacgggcgac ttggcttggc attcggcggc acgaaacgcg gaaaagtcct tttcccccac
      181 gagcagtgcc gctcctgcac gcatggcctc ggcgtcgagc ggaaggtgga accagccgac
      241 cttcccgtgc agcagcgcag gacgcacagc atggttgaac aggacatagc gatagctgcg
      301 cgatcgggcg gaaaagcgcg catgaaaatc gcccggaacc acgcgtgacc aacgcacgga
      361 gatactgtcc ggcagatgcg cgttcgcacc gcgcacccat gcattctcgg gcctgcgcgc
      421 cggaacatcg aaatgcacta cctgcgccgc cgcatgcacc ccggcatcgg tgcggcccgc
      481 gcaaacaacg cgcactggag cgccagcgat ttccgccagt gctttttcaa tgctttccag
      541 cttatcttta ttgcgcacat gcagctccgc cgacaccaag cctggcagcg gcaactcgtc
      601 cagtaccaca tcatcgccca accagggcga cactaaaccg tgaatatcgt ttttctccag
      661 aatatgaagt gccttgatat ttggattggc tgtcaaaaga gcttcaacgg aaagccccaa
      721 ctcctttatt tctactgcac tacgtaagac accgcctggc ttttgcgccg ggatttcaat
      781 tgtaaccttg tttttcaatc ccgaagacca gtgccgcgtg acgccgccca gcaccattgt
      841 cgcgctcaga gccatcatcg ccaaaaagct catgagacct accagaagca ccagaaaatg
      901 cgaccccgcg cttttatgca gaggcaagtc atacccaccc ttgcgttcac ctacgccaag
      961 cttgcgattg cgggagaaag tcggcagcaa caacgaaaaa atcttcatca atagaccccc
     1021 gcaatatttt tctgcaccca attcgcatta tcatgcatat aaagccgtcc atcctccagc
     1081 acgaggcgcg gatggccaaa acgttccata atgctctggc tgtgggttgc aatcacaatc
     1141 gtcgtgccca tccggttgag ctgttcaaac aaatgcatca accgaaaacc aatatcatca
     1201 tcaaggtttc cggtcggctc atccgccagc aaaagcttgg gccgcgcaat caccgctctc
     1261 gcaatcgcca cacgttgttg ctgtccgccc gaaagcgtca ggggcagggc gtttttataa
     1321 tcacccagac caacccattc caaaagctca tcgacattac ttttcacttc gctttcttta
     1381 tgaccggtaa tgcgcatcgg caaggcgaca ttatcaaaag ctgagagatt gggtagcagg
     1441 cggaattcct gaaacaccac gccgattttc tggcgaagta aaccaagctg cgctcggctc
     1501 atatcattga tattttgccc aaacatactc acaagaccgc gcgtcggttt acgtccaaga
     1561 tacatcaatg tcatcaacga ggtcttcccc gcacccgaag gcccggtaag aaaatgaaat
     1621 gatcccggtt ccagcgtaaa attaatgtcg gacaacactt ccggtcccac accataacgc
     1681 aaaccaacat gttcaaactt aatcaatgta ttaaattact ttcttaatat tttttctcaa
     1741 cttctcgagg cttttgcaca tccaaaatat actgcaaggc cttggctttt ttataaggat
     1801 tggtgatcgt catcgctgct gccatcgcgc cgtcgaaatc tttgcgatct gcaagaattt
     1861 tcgacacgat cgcataggcc ttattccgaa aggcgatgct atcgataaag ccgatgctct
     1921 tcatcgccgc atgcgcgttg ccgacttccg cttgaatctc cgctgtctca ccataggctt
     1981 tgtgacgcag ggcgtcattt tccatttcac tagcggtttt ccacgcccct tcattatccc
     2041 cggcaaaggc ctgagccatt gcaatatagg taagcgcaat cgcataagac ggcggatgtt
     2101 caatcttttc agcgcgtgta cgaagctttg taaacagcgc atcataggcc gctttctcaa
     2161 gcttcaaatc cgccgctgcc atgccaatcc cgcggatagt catggcttgc gtatcgggcg
     2221 tttcaatctt gtcgatcagc gcgatcgcgc cgtccatatc tccatccgca gcctttgttt
     2281 tggccaattc acgataagtt tgatcacgcc aggatgtatt ctcgatttga gctgattcgg
     2341 tttcaatttc cttgagtatg caggcacgat cttgtgccaa gcagcccgcc gcccgcagtg
     2401 ctcctgctgc atacgggaag aggctaagcg taagaatgcc aacgaataaa aatatcctgc
     2461 ccgtcattgt ttttcctcga aatgtgatat ccttgttacg tctacttgtt caaatcactg
     2521 tgtgccctta taaatactaa attaaatttc atatgagaag ctccagaaaa gaatgattct
     2581 gacttgtccc aattgtgctt cacgcttttt gctttccgcc caggtcttgg cgccggacgg
     2641 tcggcgcgtg agatgctcat cctgcggcga ggattggttc gaaatgcccg atcctgacga
     2701 gctgatcgaa aatctggaag ctaagattga gggaatcccg gaaagcgtta aaccgattcc
     2761 cgaaggctct tctgtacccg cgcttgccaa tatgcctgaa gagcgcgaag aaacgcgctc
     2821 agctattatg gcgggctatg ttggtgcact gctggtgttc gtggttattc tcgccgcgct
     2881 gctggtgatg aaacagacgc ttgtaaaaac gtggccgccc ggcgcggccc tttatgaatt
     2941 tatgggaata gaggtcaaat cgccaggaga agggctggtc tttgatcgtg taaaagccaa
     3001 agtgacaccg accgggcgca ttatcattga aggcagtatc atcaatttaa tcaatgaaga
     3061 tcagatcctc ccagcaatca aagtatccgt gcgtgaccag gctggacagg aaataaaaca
     3121 gctcacaatc cagccgcctt ttgaacaaat gaaggcggaa agcacgctac cgttcaaaac
     3181 cagctataaa gacggattaa acggcgccga tcatatccag cttcgcttcg ttctgggaga
     3241 ccctttgcaa gaaaaaactg ttgaacttaa agggttcgaa gaagagcatg ccgcagaaat
     3301 cgaaggaagt catgaagaag gaaaacatgg tgagcatact tcagagcaca cgctgccaga
     3361 acatacaccg caggaacaca acccagctcc agaatctcac cactaagatt taaaaccgtt
     3421 ttaataaccg ccgataataa tccagctctt cctgtggacg gcggcggtca ctcgcacgcc
     3481 ggcgcagctc attcaaaatc tcctgcaccc atttcctttc cgcttcactc ggaattttaa
     3541 cctgatcgcc aaacggcaag ccgtttttgc catcatgccc gccatagggt ctcccaagcg
     3601 ggtccaactg catcggcatg ccaccaaatg ccagtccggt catttgctgc atccattgtg
     3661 ccatcatttg cgccatttgc tgctgagact ctttaagttg tttaatcgct tcatcctgaa
     3721 acggaagcga aagatccggg cggctaagcc ccagttgctc accggaactg tccattgcct
     3781 gtgcggccaa ttcaaaattt tccggaattt ctcccagcgc cgtttcggcc tctttcatca
     3841 actgcacgag cgtcatatac agcgcttcct gctcagtatg ttgaacaacg gtattgataa
     3901 aaggcggcgg ttgatcttca aagctgccat caacacctag cccctcaagc ttctcataga
     3961 gctctgcttg tttagccgtt tgctcgcgca aggattcctg acgttcaatc agctcttgca
     4021 agtctttgat agctttttgc atcatctgca tatccggcgg cattgtaaag ttttttgacg
     4081 ggccagccat atccatcaat ctctgaagtt tggaaagcat cttttgtgcg gacgtccgat
     4141 cacccgaaag catatcctgc tccatctggt ccatcagacc ggccagatca ttcatattta
     4201 tcatctgcgc caggacttct ggcggcaatt gtgtcggctg catttcttct ctttgcacgg
     4261 atttctgcca ctcacgcgct aaagcctgta tatattcggc catcgcctga cgaagccgtt
     4321 ccatcagaat tgcaatctca gcgtcactga cctcagggtt ttgcagggct tgctccagtt
     4381 tcttttgaag ctcacgtaaa tcacgcgccg ccagactaag attgccatcc tccagccgca
     4441 gcgcgatgtc ccacagcaag ccaatcacac tgcgtgcaac catttccgac ggttcattat
     4501 aataaagacg cgaagccgct acccgcagcg ccagaaacac ccgtaaatca tggccgtatg
     4561 tgtgcggttg atagagtagt ttctccagcg ccagccccac ctctccataa ggctggtcag
     4621 ggttccagat cagatattta cgcatctcga tcaactgacg cgccaccgga tgtgaaaagc
     4681 tgcgttccgg taattgcagc cgcaacggca atgtttgcgc gatttgatca agatggtcat
     4741 gcgcactgaa ggtaaagacg accggaagtc ccgcccatgg atgcgcggcc agatcataaa
     4801 ccggcgccat ctgaaactcg gttttcgctg gcgacataac cgagcgctct tcctgcgcgg
     4861 cccagcccat cggggctttg tctacgctgg ggtccaacgt catgcgcata tggagggttt
     4921 taacgccata atcatcgagc atcgtcagtg caaactggac ttgaccgttc tcaagcgcgt
     4981 gcgggtcctc tgtaatgcct atgctgggtg gcaaatcttc aatgaattca taatcccaaa
     5041 ccggcccgcc ccacagcccc gtgcgcaggc gtaatttatg ccccaccgga atctccattt
     5101 ccagcgtgga cgcgccatct tcaccacttt caaaggggta agcttttcca tccaccagca
     5161 gggcgggcat acgcagggca tgcaacaggc tgcgcgttgc aatcgccttg gcgacagaac
     5221 cttgcggaat gtgcaaaact ccatctgttt ccggcggaaa aatgcgcggt tgagtggtat
     5281 attcgggcgg cgtaatccaa aaggcgattt ccggcgcttg gcgctcaaat atctccagcc
     5341 cgccgaccgg ccatagcccg cttaaaatcc gccgcttacc gtccgcacct gcaacgataa
     5401 ccccgcagat aagcgcaatc agtaagccta atcgcaatgc ccgcaggtcc cgcgccgcca
     5461 gcaatgcgcg ggggagagca gggcgcagtt ttgcaagcat ctgcattttt cggctttgct
     5521 cccatgccca cagcgcattt acgcctgctt gtacagggcg gtctcctaat gtgcttaaag
     5581 gacgatggtg tacgccgctt acttgctcaa tgcgccgctg aacttccgta cgtttcggcc
     5641 agcgaaaaga cctgatgtct ttgcgaataa aatgcaccag tccaatgaag aaaattagag
     5701 tggtaattgc agagacaacg gcgcccagcc cttgcggaat ttcaaacagc cacagcgcgc
     5761 caaaccccat gcaccagctt gccagccgcc agagggccat gccaagcctt tcaatcatca
     5821 gcgccgcacg gctgataaag tgcagccatg ccaaattgac gcccttattt cgcaaaaatt
     5881 ctggatttgc actcataata ttgagtgtaa gcgagtcctt caaaaaagcc agtttctaaa
     5941 ccagccaatc aggaattata tctgcaccaa gcaggtcttc atagctttgc cgcgcgcgaa
     6001 taagtgcaat cttgtccccg tcaaccagaa tttctggcgg caaggggcgc gtattataat
     6061 ttgaggccat acaaacccca tacgccccgg ctgtacgaat aacggccagg tctccgggct
     6121 tcatttccgg caggtttcgt tgcttggcaa atacatcact gctttcacag accggtccga
     6181 cgatattgta ggggtgcagc gcggcatccc ggtttgctac gggctcaatc ccatgccacg
     6241 cgccgtaaag ggcagggcgc attaaatcat tcatcgcgcc gtccacaacc agaaaatcct
     6301 ccccatatga ttctttttca tacagcactt tggtgaggag cacgccggca ttgcccacca
     6361 gatagcgccc cggttccata atgatttccg tatccagcgg caggatatgc tcgcgcacgg
     6421 cattggcgta ggaatcaaga tcgagcaaat cttcatcctg atattgaatc gggaatcctc
     6481 cgccaatatc cagccgcgaa acttcgtact ctttgctgcg caaaagaccg acaaaatcag
     6541 caatcttttt gaacagagcc tcaaaactgc cagtctcagt gatttgcgag ccgatatgcg
     6601 taaaaatacc cagcgcccgc acatgttcca tgctctgagc gcgcgcgaat ccctccaaga
     6661 cccgctcagg cgccagaccg aatttatcgc ccttactgcc tgtgctgatt ttcttataag
     6721 ctgccccgcc cacatccgga ttcagacgaa acaccacatc ggcgagttgc ttttcctgcc
     6781 cggctaaacg gttgatcaga tccaactcgc caaaggattc gatgttaaat tgatgcaccc
     6841 gtgcgcgcag ccccgccaga atttcctctt cggttttgcc aacgccttcc attacaattt
     6901 tttccggcgc aaatccggct ttcagggcgc gcttcaattc tccaccagaa acaacttcaa
     6961 tgctggagcc taaagattgc agcagcttca aaatgcctaa attgctattc gctttacaag
     7021 cataacacag taaaggctga cgattcgctg gtaaagtttt ttgtagcgct ccggctaatg
     7081 catcatactg atcacgaata acacgagccg aatagacata cgccggtgtg ccgtatttct
     7141 ttgcaatatc actcaaaggt actgaatcga ggcaaagtac gccgttttgt tcgtgaaaac
     7201 cactcatcgt cttaactttc aggtcttgga tctgtactca aatcggggta tgtgcgcgga
     7261 aacttgctat cctgagcgcc ttccgatggc aaaacttctc ccggcttcac accacatccg
     7321 gccgttatca ggcataaact aagcaacaat aatgaccaac ttcttatcat acgggatctg
     7381 cccttatttc taaatttgag caatttttca atgtttataa gataataggg gccaaaatac
     7441 tatcagcaat atataaaaaa ctgcgtatag cactggcgaa tttcttaaaa aaatgtacac
     7501 ttttatccag tagactattt aaatgactat ctgtttataa gccatttcaa gaacataaat
     7561 agccacatat gcaggaacag aacgcgcaat cgcctacgct ggagagcatc tcccaagatc
     7621 agcttgatgc gttggtcaaa ctccacaacc gctttttgga tgggcgtttg ggcggtcgcc
     7681 gtgcactttt aaaaaatacg gatttaacgg gcttgtcttt gcgggcgcag gatctccgtc
     7741 aggccagctt tatgggttgc attatggtcg gcatggactt atcacgtgcc aatttccagg
     7801 aagcctctct ttatgcttgc gatctcagca attcgaatct gaacgacacg cgctttgtcc
     7861 gtgctgattt gcgcggtgcg cgaatcgaaa acgcaacact gcagggcgcc gaccttgaaa
     7921 aggcggattt gcgcgtaggt ggtctgtccg atggtcaaag ttatgacagc ggccaggcag
     7981 taaatttccg tggcgcaaat ctaagtggag caaaactcgc tggttccatc gcttcaaaag
     8041 ccaatttttc cgatgcgatt atgaccggtg caaacatgtc taaagctgat ttgcgtggcg
     8101 ctcagatgga aggcgccgat ctgagcgggg cagatattga tggggcacaa ctcagcgggg
     8161 caaatttcaa atctgcaatt ctcacaggcg tagaagtcaa aaaaattcgt gagaccttgg
     8221 atatagacct ttctgatgca attacggatg aaaatatcgg tgcatcggtc gccgatctcg
     8281 atgagccgtt agccaagctg attgaagatc atcgcggctg ggtggaaacc gcaggggcga
     8341 aggggcggca gcttgatctt agtgatatgg atatgcgcag cttaagaaca ctcaagatgg
     8401 aaagaatgac cgcgattcgc gccatcggta cgaagttttt agggatgaat ctctacaaga
     8461 tcgaactgca aagcgcggtt tgtgacgggg cggatttccg caactgcgat atggaggagg
     8521 cagatctacg cggctccagc ttcgcaggtg caaatttttc ccatacaaaa atgaaaggtg
     8581 tagacggcag cccactgatg tttggtgccg aaggggtgag caaacgcttt agcccctgta
     8641 attttgaaaa tgccaaatta cgctatgtgg acttaagcgg cgcacagctc aaaagcgccg
     8701 tgttcaaagg cgccgatcta tcctacgcag acctttccgg cgcggattta cgcgagagtg
     8761 acttcacgga tgccaacgtc acaggcacaa attttgatgg tgcgcagacc gaaggcgcca
     8821 acatcccgaa agccgataag cccgtgtttc agatgaaacg ggactgaaca gccgttcaat
     8881 tttgttttta ataaggcttc tcctctccat tattaggtta atgaaatctt aaattttgca
     8941 ttccataatg taaaaaacga gaggtcggaa tgactgtgaa ctacgaaaag gcagaaaata
     9001 aagccggtga caatgatgcc gaggcagttg tcgaaacagt cacggaggtg gcggcaggtg
     9061 ctgtaactgt ctcagttccg gctggaggcg ctctcgtctc tgcattcaag gacgttgata
     9121 atcttccatt aaaccttata aaaaaaatgg gttctaaaag actgccatgg ctgttgggta
     9181 ctgcagccac tgccggtttt gtcgtcgatg aagtcgaagc gcattgggaa aagggagatg
     9241 tatgtaaggc ttttggtgcg gctttggcag gaatagctga gacaggggga aattttgtgc
     9301 ctggtggaaa ctatttgtta ctcggtgaca cgtttcacaa aaccgtacgg acggcctata
     9361 tcggggtaac ggaaggagta ctcggatttg aagaagggca atgcacgcca aacgaaacag
     9421 atattgtggc tgcggttgat tatttatcct cggacggtgc accccgcatg gtgatgaaca
     9481 taaacgatga aaaattggcg ccgtaacgca ctatttttac aacttctcgc tcaactccgg
     9541 cacggcgtcg aacaggtcgg cgaccagccc gtaatcggct acggaaaaaa tcggcgcgtc
     9601 ttcgtctttg ttaatcgcta caatcacttt ggaatccttc atcccggcta aatgctggat
     9661 cgccccggaa atgccaacag cgatatacag ggcaggggcc acaaccttac cagtttgccc
     9721 gacctgataa tcattcggca cgtatcccgc atccacggct gcgcgtgatg cgcccacagc
     9781 cgcgccgagc ttatcggcca gtttttcaat caacgcgaaa tcttcttttg aaccaacgcc
     9841 gcgcccgccg gaaacgacga tattcgccga tgtcagctca gggcggtcgc tcttgctcac
     9901 ttccagattg acgaaagagg acagccccgc atcgcccttg gccgcaatat cttcaatcgc
     9961 tgcgctaccg ccctcaggcg aaaccgcgtc gaaatctgtg ccgcgtgccg tgataacctt
    10021 gattgcatct gaagatttca ccgtcgcaag cgcattgccc gcataaactg ggcgttcaaa
    10081 cgtatcggca tcaatcactg cggaaatatc ggaaatctgc tgcacatcca gcaaggccgc
    10141 cacccgcggc agaatgttct tcgcataggt tccggaagct ccaataatgt gcgaatagcc
    10201 agaagccagt tcgacaatca gcggcgcaac attttccgcc agcccgtatt ccagttccgg
    10261 cgcatccgcc ttcaaaactt tactcacccc ttcggccttg gctactgctt cagcaacgct
    10321 cgcgcagttc gcgcccgcca ccagcacatg gatttctccg ccaatcttct gcgccgcccc
    10381 aatcgcgctc aatgttgcag gcttcaattc agcattatcg tgtttagcta aaacaagtat
    10441 ggtcatttta aatcactttc gcttcattct ttaatttatc gatcaattca tcaaccgatg
    10501 aaaccttacc gccgccttga cgtacgggcg gctcagccac ctttacgacc tccagccgtg
    10561 gagtaatatc gacgcccagt tcatcggggt ttgttacatc caaaggcttc tttttcgcct
    10621 tcataatatc gggcaacttg gcatagcgcg gctcattcaa tcgtaaatcc gtcgtcacga
    10681 ccgcaggcat cttcaaagcc acggtctgta aaccgccatc aacctcacgc gtcacgttaa
    10741 gtgagtcctc atcaagcgtt acctcagaag caaaagtccc ctgcgcccac cccaacagcg
    10801 cggccagcat ttgtccggtc tggttggaat catcatcaat cgattgcttg cccataatca
    10861 ccagatccgg gttttctttc tcaaccaaag ctttgagcat cttggccacc gccaaaggct
    10921 ccaccccgcc cagatcaatc ggcgcatcgg tctgcaccaa aatcccgcga tccgcaccga
    10981 tcgccatcgc cgtgcgcagc tgttcctgcg ctttttccgg gccgatcgat acgacgacaa
    11041 tctcgtcagc tttgcctgct tccttcagtc gcacagcctc ttcaatcgca atttcatcaa
    11101 acggattaac cgacattttg acattggcca gatcaacccc actgccatcc gccttaacac
    11161 gcaccttaac gttgtaatca acaacccgtt taatcgggac cagtattttc attataatat
    11221 ctccacttgg aactgtggtt taagaactct gagaagcata gtatgccagc gccagcaggc
    11281 tcaaggcaac cccttgcaga acgacccgcg cctgcatcaa acgattgccg tattttttat
    11341 taaattctcc gcccttgatc atggcgaata atccaatcgc cagcaccgcc agaacgcaaa
    11401 gcatggcaaa aacaattgat gtcgcaagaa gtccgttcat agctctaggc taattgagag
    11461 aagggggctt gaccagagta aaatatagga aattatttga cataatacac aaaattggtt
    11521 aatattagct taaattaaac aagcgttaac tgttaagaag ataaaaaatg caaagcaaac
    11581 accacgaaaa ttggacttac aaatggtcaa acaccagtat gggcaacagt tttgcgatgt
    11641 ttaactgtcc tgaaatcgga acgaatatac tctacacttt tgaacaaatt aaagaaattc
    11701 tactcaataa taaaacgacc aacattccct acagcgtgtt ggctgaagca gcaaacgtat
    11761 atcttggtgt taaatcagta gcttaatgat ttttaaaccg ccttttgagt actcataaga
    11821 tagttaacgt ccaaatcact ttccgatagc tcaaattcat ttttaaacgg gttaaagaca
    11881 aggccggtaa gattgcaggc ttccaggccc gctttctcta ccattttaac aagttcggat
    11941 ggtctcaaaa attttttcca gctatgcgtc tcacgcggaa cccaacgcaa aagatattct
    12001 gccgtcacaa tccccatcag aaacgatttc ggattgcggt tcagagtcga aaaaatcact
    12061 atcccgccgg gttttacaag attcgcaaca ttttgcacga aggtttcagg agacgtcaca
    12121 tgttcaataa tttccagcgc catgacaaca tcgaatttct gctcaatagc ctcagccggt
    12181 ttattgagat aagcgatttt taaaccgctg gtctgtgcat gtctcttggc ggtttcaata
    12241 gccactgcat ccgcatccgc cccgctaacc gttcccccca ttcgtgccag aggctcacag
    12301 acaagtccgc cgccgcaccc gacatcaaga aagcttaacc cctcaaaagg cctcaagctt
    12361 tccacatcgc gcccgtaatg acggcaaatc tgcgacttaa tataacgcat gcgcacagga
    12421 ttgaggcggt gcaatggctt aaaagggccg ttttcatccc accattgttc cgcatcttta
    12481 gaaaaatttt caatttcttg cgtatctata ctggacataa gctttagctt ccttattaat
    12541 acgagagttt tttaaccgat ttcgaaaaaa aaagatatgg ctttaatcgt tgcaaaattt
    12601 ggtgggacct ccgttgccga tgttgaacgc atccagcgcg ctgcgggtaa aatcgcccgt
    12661 gaagtcgagg cgggacatca ggttgtggtt gtggtctcgg ccatgtccgg cgtgaccaat
    12721 cagcttgtag gttactgtaa tgatattagt gagctgcacg atttgtatga atatgacgtc
    12781 gtagtttcca gtggtgagca ggtcactgcc ggacttatgg cgatggcgct gcataagcac
    12841 ggcatcaccg cgcgcagttg gctcggctgg caaattccgt tcaaaaccga tgaaatgcac
    12901 tccaaagccc gtgtgcgcga cattgaaaca gcaggactcc ttgaaggtct taaaaaaggt
    12961 gaagtcgccg ttgtgccggg atttcaggga ataacagccg ccgggagaat atcaacgctg
    13021 ggacgcggcg gttcggacac ttcagccgtt gcactggccg ccgctttgaa tgcagatcgc
    13081 tgcgatattt acaccgatgt tgatggtatc tacacgaccg atccgcgaat tgtatcaagt
    13141 gcgcgtaaac tcaagaaaat ttcatttgaa gaaatgctgg aactggcctc gctcggctca
    13201 aaagttttgc aaacccgctc tgttgaaatt gccatgaagc atggggtgtc tttgcaggtt
    13261 ttatccagtt ttgaagatca aattggcagt gatatgccag gaacgctcgt tgtcaatgag
    13321 gagaaaaata tggaagaaga actcgtcagc ggtatcgcct gtaagcgcga tgaagctaaa
    13381 atcacgctgc tgcgcgtggc agatgtgccg ggcattgcag caaaagtttt tagccccctc
    13441 ggccgcgccg gggtcaatgt cgatatgatc gtgcagaata tttcagccga cggcaaagcc
    13501 accgatatga cttttaccgt gtcgcgcacc gatttaccac gcgccttgga agctttgaaa
    13561 gatattgata tcgaatatga agaaatacgc tcgtcaggta atgttgcaaa aatttctatt
    13621 gtcggtgtag ggatgatcag ccatcctggt gtagctgaaa aaatgttcac cacacttgca
    13681 gaaaaaaaca tcaatatcca cgttatctcg acgtctgaaa tcaaaatcag cgtcttgata
    13741 gaggaagact acgcagagct ggctgtccgc gccttgcata atgcctatgg actggaagag
    13801 gcggcataaa taagttactc tgcctatatg atggccaacc ccgcaaccga gcttcaagat
    13861 actacttttt ttaccgatat gcctgtcggc gaaatcctgc gccgcacacg tacgcactac
    13921 aaacgctcaa tcgaagatat tgagcatgcc ttaaggattc gcacagaaca aatcgaagcg
    13981 attgagcagg gtaatcttga gatgcttccc ggtcgtgttt acgccatagg ctttgtccgt
    14041 agctatgctg aatatcttgg cctggatggt gacaaaatgg ttgatcttta caaacagcag
    14101 gtcatggctg ctaaagggcc agagccggag ctttatttcc ccattcctgc atctgataat
    14161 catctgccgc cctggtggat tacactctca tctattttcc ttgccattgc cctgctggct
    14221 ttctggtttg gagagaaccg cgctggccgc aaggccgtag aaaccattcc tcccgtgcct
    14281 gaagaaatac agattgctgt cgaagaaccc acaccgcccg ttgccagcaa agttgaaatc
    14341 gaagagcctg aagaggccgt aatagaagac aagcctgttg aaggcattat tctgaaaata
    14401 cacgagaaca gctgggttga aatccgtgat aaaaaaggca aggccatgct ttcgcgtgtc
    14461 cttaaggcag gcgatcaata ttatgtacct gaccgcccgg atttaacaat atcaatcggc
    14521 aatgcaggcg gcgtcgagat tgaggttgac ggccagcctt tgcgaaaact tggcgaaaaa
    14581 ggcaaggtgt tgcggcgctt gtcgcttgat gcggattacc tgaaaagtaa ttttgcagtt
    14641 gaaagagcac agtaaaccac tgtaaatcat cactatgtct ttagaagaaa aactcgtatc
    14701 catcaaaaac cgccacgcag agcttgctgc tctcatgtcg gaaggcggcc tcagcggtga
    14761 gaagtttgcc aagctttcga tggagtatgc cgagattgat ccggttgtgg aagccattac
    14821 cacgctggca gaggccaagg ccgaacgcaa agacttgcag gaaatgcttg ccgatcctga
    14881 aatgaaaaac atggccgccg aggagatttg tgcattggac gcgaaaatgc caaagctgga
    14941 gcatcaaatt cgccttgctc ttatccctaa agatgaagat gacactaaaa acgctattgt
    15001 tgagatcaga gccggaaccg gcggcgatga ggctgcgctg tttgttggtg acctgtttgc
    15061 catgatcaaa ggctacgcca ttaaacaggg ctggaaactg gaggtaatag aaacttccca
    15121 atccgatgca ggcggctata aagagattgt ctgcgaaatt agcggccaca acgtcttttc
    15181 caagttaaaa tatgaatccg gcactcaccg cgtccagcgt gttcctaaaa tcgaaagcca
    15241 gggccgcatt catacctccg ccgccaccat cgccgtgtta cccgaggccg aagaggctga
    15301 agtcaacatc cacagcgatg atctacgcat tgatacatat cgttcccagg gtgcaggggg
    15361 gcagcacgtc aacaccaccg attctgctgt gcgtatcacg catattccta ccggcgtggt
    15421 tgtagagatg caggaagaac gctctcagca caaaaacaag gccaaggcga tgaaggtgct
    15481 atcaacccgc atctacgaca tacaacgcca gaaacttcac gatgagcgtg cacaggaccg
    15541 taagagccag gtcggctccg gcgatcgttc ggaacgcatc cgcacctata attttccaca
    15601 aggacgcgta accgaccata ggatcaattt aacgctctat aaactcgaag aggtgctcag
    15661 cggcgaaact ttaagcgaaa tcaccgaggc cctgatcgcc gaagatcagg caagcgcctt
    15721 ggcctcggtg gagaattaac ctacggcgca gccgctataa aacaaatgaa actcaatatt
    15781 ctttataaaa ctctgcgcca aagcctttct gaatccgacg cacactacgt catccaaaag
    15841 cgcacaggtc tgaaccgcgc tgatatgatt tccaatcctg atcaaatcgt agacaagtta
    15901 caaattctta aagatcttca acgtcaccaa aatggcgaac cactttcacg tatttatgaa
    15961 gagcgtgaat tctggagttt atcattttcg ctgtctcccg atacgcttga tcctcgtttt
    16021 gatacagaaa cgctgattga ggcagtgttg gcacgttaca aaagcgctcc tccaaaacgc
    16081 attctcgatc tgggtacagg cacaggatgc atccttatcg ccctgttatc ggagtttccc
    16141 gaggctactg gagtaggtgt agatctatcc gaaggcgcgc ttaagacagc ccgctccaac
    16201 gccgaagcca acaaagtcga gaatcgcgca gcatttatac aaagcaattg ggccgaatgc
    16261 atcgacgaat cttttgatct ggtggtttcg aatccccctt atgtttctcc aaaagcgatt
    16321 cagaatcttg ataaatcagt ccagaattac gatccgattc tggctttgca tggaggcgca
    16381 gatggtttgc aagcctataa agaaattttt tccgatcttt ttcgcctgtt aaatcccggc
    16441 gcacgggctt tttttgagat cggattcgac cagcatgatt ctgtaatgcg actcggcgaa
    16501 gaatcgcggt ttttggtgga aggcttatat ctcgatttag cagggcacat aagggtttta
    16561 gagctaacgc cacaaaacag tgatggggat aaagtcaaaa ataatttgcc ggacggttga
    16621 ttgaaaatac ctatcggctg taggttaaaa atcatcaacg cagacaaacg caaatatggt
    16681 gtttggtgtt gagtgaataa accctagata tagtgtgttt gagcctcaag gggctagcca
    16741 tacgtacgca aataatgtat ggtgtacgtg tatgtgcaaa aataaatatg aacggaaaat
    16801 gaaaaaatga gacacggaac ttcaaataga cgtcagcgcg gcagagggaa tggtcagcgt
    16861 cgaggaaata atcagcggat gcaggtttac gacagcaacg gccctgacgt acgtattcgt
    16921 ggcaccgccc atcaggtgac ggaaaaatat acggctttgg caaaggacgc cgcttccggc
    16981 ggcgaccgca tcttggccga aagctacctc caatatgccg agcattacca gcgcatcata
    17041 aacagctggg aagaaggcgt acaggatgat aagcttcgta cttcggaaac acaggaaaat
    17101 agccagaaaa aatccaaagt gagcgataat gacgatgatt tatcattgcc ttccagtata
    17161 ttaggagaaa aacataatat aaaatctgag gagtacgcaa cagaagcatc tgcgtaatac
    17221 acaggttgta tgtgtctgat ttaaaggcgg ttttttgacc gcctttttaa tgtttaacgc
    17281 ataaaagttc ttcctttcaa aacttgacta aaatcattga aaagttgcgg tagaatccta
    17341 taatccctcc tatgaatttt gaaaaattta cagagaagtc aaaagggttt attcaagcag
    17401 cgcagacact tgcgatgcgc tatgggcacc aatcaattga
//
LOCUS       DAOSWR010000031        17120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2104_length_17120_cov_5.704893, whole genome shotgun sequence.
ACCESSION   DAOSWR010000031 DAOSWR010000000
VERSION     DAOSWR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 17120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17120
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_17120_cov_5.704893"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="dnaN"
                     /locus_tag="PK513_03790"
     CDS             complement(<1..1083)
                     /gene="dnaN"
                     /locus_tag="PK513_03790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOO81605.1"
                     /translation="MKLSIERASLLRSLGHVQSVVERRNTIPILSNVLMKAEDGVLSL
                     ATTDMDLEIDESVAADIKEPGATTVPAHTLHDIVRKLPEDSDVELTLNAEGTQMSVKA
                     GRSQFKLSCLQTTDFPEIGAGDLPNGFAIPAADLRALIDRTKFAMSTEETRYYLNGIY
                     LHEADQDGVKVLRAVATDGHRLARFEMPLPEGAGGMPGIIIPRKTIGELRKLVDEAAD
                     AIQITLSESKIRFAFDHIVLTSKLIDGTFPDYQRVIPAGNDKIVEVDAKMLTSAVDRV
                     STISDGKSRAIKIALNGKQMTVSASSAESGSATEEVEVNGETDIEIGFNAKYLLDITS
                     QIEGEGCRLTLADPASPTIIQDNSDPS"
     gene            complement(1212..2525)
                     /locus_tag="PK513_03795"
     CDS             complement(1212..2525)
                     /locus_tag="PK513_03795"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOO81606.1"
                     /translation="MRRISPIWHPMTQHKICGPALHVDSAAGARLKLRGGQEIIDGIS
                     SWWVNTHGHCHPKIVEAVRAQAGKLEQVIFAGFTHDPAEKLTRKLLSTVHGTFEGKDG
                     SALDYVFYSDSGSTAIEVALKMAIGFHENAGEQRHKVIALEGGYHGDTFGAMAAGSRS
                     VFNVLYEPFLFDVKHVPFPKTGEEMRSLAWLDKWLKKFGSQTAAFVFEPLVQGAAGMR
                     VYSPKVLGKMAQMCREHGVLLIADEVMTGFGRTGSMFACEQADFTPDLLCLSKGLTGG
                     FLPMGATLATEEIYKAFYHDEKAKQFFHSTSFTGNPLACVAALASLEIWDEEPVMEQI
                     AAIEASHLKAAGWYSARPDVMDVRVKGSIFAIDVRDDVNGYLSSIGHEIYDFMLANGV
                     LLRPIGNTVYILPPYCIDQQDLECIYETLWRSLDSFRNDCFQQAA"
     gene            complement(2526..3173)
                     /gene="bioD"
                     /locus_tag="PK513_03800"
     CDS             complement(2526..3173)
                     /gene="bioD"
                     /locus_tag="PK513_03800"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOO81607.1"
                     /translation="MSNRCIITGTDTGIGKTVVSAMLALALGATYWKPVQSGVEGGVD
                     TRAVQKMTGLPLERFLPESYVFTESLSPHRAAELDEAEIDVESLVIPEVDGPLIIEGA
                     GGLMVPLTRQNLLVNLFKRWNEDPVNGGRVPVILVARTGLGTINHTLLSLEALWSRKI
                     PVHGIIFVGDENEDNMRTIGEFSDVKVLGRLPMLDSLDAKSLTDAFEQNFDLSDF"
     gene            complement(3174..4373)
                     /locus_tag="PK513_03805"
     CDS             complement(3174..4373)
                     /locus_tag="PK513_03805"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOO81608.1"
                     /translation="MMRDFRQTLDRLGQRGRYRTLMLPAGIDLTSNDYLGLASSVYLR
                     ECALKFLQNGGVIGAGGSRLLRGHTDAHAALEDFAAGYFSAPKSLYFATGFQANSALF
                     QTLCTRHDTIIFDEFVHASAREGIQNSNAQHIKAVHNDVNSFEEALRKAKAQQKKDGQ
                     IWVAVESVYSMDGDIAPLKALYGLAEDYDAVLVVDEAHATGVMGRAGKGLAYELYSSL
                     RAQRSNPEKIDCFVADTPRNDVWPDNLIVLHTCGKAIGVAGGLVCGSEAIIDFMINAA
                     RGFIYSTAPLPLQVHLVQKSLEWIASEEGEIARKKLAGLSRKAQQSFGGPGTHIVPIM
                     LGDDAYALDVAQKLQQKGYDIRAIRPPTVPEGTARLRLSLSAALDKVRLDQFAADLDG
                     FLERKAA"
     gene            4831..5445
                     /locus_tag="PK513_03810"
     CDS             4831..5445
                     /locus_tag="PK513_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO81609.1"
                     /translation="MARRNDHTREELKSLTLERALKIVKNEGFQKLTARRIAKEINYT
                     PGTIYNMFGSMDGLYFALNAHTLDKLFETIAAPEQYPAEATISENIKTIAKSYMDFAQ
                     NNKELWLMLFNMSFPTKDKAPDWYREKVQSILDILENLLAPLFSENQTREKAIAARTI
                     WSSVHGIYLMEQTNKIGFMSDKSALDMAECLIDNFIAGLQQNAA"
     gene            complement(5455..6471)
                     /gene="bioB"
                     /locus_tag="PK513_03815"
     CDS             complement(5455..6471)
                     /gene="bioB"
                     /locus_tag="PK513_03815"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOO81610.1"
                     /translation="MTHTLEEVRALYHRPLMDLVYDAATVHRTNHDHYEMQMCTLLSI
                     KTGGCTEDCGYCSQSVHNEAELEKEILMKTEAVLEEARKAKEAGSTRFCMGAAWPRVL
                     EGRAFDRVCDMVKGVSDMGMESCVTLGMLTEEQAKRLKEAGLKAYNHNLDTSEEFYDK
                     IITTRSYQDRLDTLGNVAKAGISACCGGILGMGESEEDRISLLHTLCNLDPQPESVPI
                     NMLVPVKGTPLEHALPLDIFEWIRAIAVARILMPKSMVRLSAGRLEISKEAQALAFFA
                     GANAIFTGEKLLTTSNPEKDFDHALLDELGMKPRAPFKDDSAERKSGGCGKPGGCGCQ
                     KEAA"
     gene            complement(6562..7908)
                     /gene="dnaA"
                     /locus_tag="PK513_03820"
     CDS             complement(6562..7908)
                     /gene="dnaA"
                     /locus_tag="PK513_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOO81611.1"
                     /translation="MKLWKKVHNDMRSEFGEAIFRSWLKPLTLQAFYHGTMEVSVPTR
                     FMRDWIQNHYAERILGMCIEASEGFEDGVKRLQIVVVQNAIVDDEPETAAPSKANQNK
                     KLDISEISSPLDERFTFETFVVGKPNALAHAAARRVVESLTIPFNPLFIYGGVGLGKT
                     HLMHAIAHAIEEQSPEKTVMYLSAEKFMYQFVKALRSDSTMMFKEKFRSVDVLMIDDI
                     QFIAGKEATQEEFFHTFNALVDQNKQIIISADKAPSDLSGLDERLRSRLAWGLVADIH
                     PTTYDLRLGILQTKRAQLGADVPDSVLEFLALKVTSNIRELEGALNRIVAHADVTKAE
                     VTLESTQEVLQDLLRAHDRRITIDEIQRKVAEHYNLRMADMHSARRARNVARPRQVAM
                     YLAKQLTARSLPEIGRKFGGRDHTTVMHAVRKVEELIEEDAQIAQDVDVVRRALTG"
     gene            8145..8453
                     /locus_tag="PK513_03825"
     CDS             8145..8453
                     /locus_tag="PK513_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81612.1"
                     /translation="MSSEPNSKPKAAILATNNSTAPIPKTLLIKRRQTSNQNKTLLKH
                     NTHKSMLQKTSSRPIRPLFCPDRNKPTLKKYVDAIRTKQENQKRNFFIFFYTIDFKHI
                     "
     gene            complement(8550..8822)
                     /gene="rpsT"
                     /locus_tag="PK513_03830"
     CDS             complement(8550..8822)
                     /gene="rpsT"
                     /locus_tag="PK513_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOO81613.1"
                     /translation="MANHKSSKKRIRRNDKRAAINGARMSRIRTFIKKVELALVAGDA
                     KEAESALQNAQPEISRGVVKGILHKNTAARKVSRLSSRIKLLKKAA"
     gene            complement(9043..9417)
                     /locus_tag="PK513_03835"
     CDS             complement(9043..9417)
                     /locus_tag="PK513_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81614.1"
                     /translation="MRIFVIIAVMILAGMVMASASVYAQDDSVQFFEVLDDVPLMPGL
                     IELPERSMSFDKPGGRVVESVAEMAAMSVDSVRAYYASVLPQFGWNPVGGDQFIRNGE
                     RLEMMFEDEAGIIILRLSIQPK"
     gene            complement(9430..10275)
                     /gene="mutM"
                     /locus_tag="PK513_03840"
     CDS             complement(9430..10275)
                     /gene="mutM"
                     /locus_tag="PK513_03840"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOO81615.1"
                     /translation="MPELPEVEIVKRALEPAMVGRRVKRLVFNRADLRFPLPQGLPAA
                     LQGQRIECLLRRGKYILGFAENGAGFVLHLGMSGAVRIEPPGSISLHEKHDHVEFYMD
                     EGLRVVLNDPRRFGFLDAVGREDCQDYPALAAMGPEPLGNDFNAEILAVTIKGRKTPI
                     KSALLDQRIVAGLGNIYVCEALYRAAIDPRRKAGDLNAKECTALCMSIRSVLRDAIEA
                     GGSSLKDYKHTDGQLGYFQHHFAVYDRVGQGCGACECDIVETGGVQRIVQAGRSTFFC
                     PEKQG"
     gene            10754..11404
                     /locus_tag="PK513_03845"
     CDS             10754..11404
                     /locus_tag="PK513_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81616.1"
                     /translation="MPTIEQIRAARALLGWNQHELADKAGLSQTGIARIENGTNQPNS
                     KTLEKIGTAFDHADIEFIGDTGLRKRSIQSQTYRGIEGFKEFLDNVYHTARDVGGELC
                     TFNAATPYFKTMLGAEWYEQHAERMQAIRDNFTMRLIVEEGTKDLLAKGFGEYKFFPK
                     NLLTDQKIYVYGDFVSFMNFANENVEIVEVKHGPSAESFRILFNIAWEKVATSLKE"
     gene            11490..13661
                     /locus_tag="PK513_03850"
     CDS             11490..13661
                     /locus_tag="PK513_03850"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO81617.1"
                     /translation="MSESDNTETSPVPEGAKSAFAAANGTNGHGQINVAIIGAGSVGV
                     FTAIEVAKNGARVSLVSRPGSQTLQALKQQKYIELASTKTTREIDAMFEAKGNYISPP
                     AEISDIPQDDDLTEVIGDKRVISHGKLADKLKKMGNAVGNELFGAVNAQTQKEYFTQG
                     EIHTLLTKHLRIPLYVQERDGDEEAYRKALGSTRYVKLFDNIKDAEPENQVIIVATKS
                     FSKSAEFAKRTVLPFLAENGVVVDLSNGLKRSSIPKLERTNLRQTLHEALTESDVLAD
                     LNAFEDEIGPERIIGANALFAVEAEKNSPHRVHVRSLLHTAAIYTANTNPYVERIFEG
                     SDIRPSTPHDPLAQSLDKLGINMMNIMSAVYDKSKAEVFSNQTLLNLYNNALRELYRV
                     AETGYGVTLHRGGPEAYIEHCQRYHRRAVLAASHAGDIHRSSTHKDIAEGKAKTEREF
                     LLDAIIELSDKQKIQVPHLRILSSFMDTIEELIKENQLDELRKQHYIFYENTHEMRDG
                     TVTRVHPPKTSKDVELPNVGTVVTSCKMPFFEWRRLHNNRPDYFRNIMPQLLHAVDPN
                     HAVFKNGKCVELMQAFVDSKDVNPETGEINLESSAFNVFWETIKTYKEQWDAYTAAKK
                     NDEKPEEKPEAKRKRVELAWQAVNDAKNELEQRYRNARTLTNNRGGAGTGGATTITAD
                     FTHANDDANPWLNKKRGVRKILPKYSTFDGPYSTFDGPGCF"
     gene            complement(13864..15519)
                     /gene="groL"
                     /locus_tag="PK513_03855"
     CDS             complement(13864..15519)
                     /gene="groL"
                     /locus_tag="PK513_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299354.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOO81618.1"
                     /translation="MSAKDVKLGGDAREDMLAGINVLANAVKVTLGPKGRNVVIEKSF
                     GAPRTTKDGVTVAKEIELSNKRQNTGAQLIKEVASKANDHAGDGTTTATVLAQSIANE
                     GAKAVSAGRNPMDLKRGIDMAVAKVVEDLAKRAKNVKGNEEIKQVGTISANGEESIGA
                     LLAEAMDKVGNEGVITVEEAKSLDNELEVVEGMQFDRGYLSPYFITDADKMQANLENP
                     YILLNEGKLSNLQALVPILEAVVQSGRPLLIVAEDVEGEALATLVVNKLRGGLKIAAV
                     KAPGFGDRRKAMLEDMAILTGGQVISEDLGIKLENVTLDMLGSAKKVSITKDDTTVVD
                     GAGDKGDIDARCAQIRTQVEATDSDYDKEKLQERLAKLSGGVAVLRVGGVTEVEVKER
                     KDRVDDALHATRAAVEEGIIAGGGTALLYATKALEALEGDNDDQTVGIEIIRRALQAP
                     IRQIAQNAGAEGSVIVGKLLEQKDTNYGYDAQAGEFCDLVKAGIIDPVKVVRTALQDA
                     ASVSSLLITTEAIITEAPQEDKGGAGMGDMGGMGGMGGMSGMM"
     gene            complement(15594..15890)
                     /gene="groES"
                     /locus_tag="PK513_03860"
     CDS             complement(15594..15890)
                     /gene="groES"
                     /locus_tag="PK513_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026019247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOO81619.1"
                     /translation="MAKFRPLHDRVLVRRVEADTKTAGGIIIPDTASEKPMEGEIVAV
                     GSGHVNDNGDVRPLDVKEGDNVIFSKWAGTEVTIDGEELMVMKESDIIGIVDAA"
     gene            16183..16446
                     /locus_tag="PK513_03865"
     CDS             16183..16446
                     /locus_tag="PK513_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Usg family protein"
                     /protein_id="HOO81620.1"
                     /translation="MADLTKQFCNYRLTTAKILYHMPDYERILQEFIWQEYDLAPQFP
                     ELFKFLDFWEKKIEGRLHSVYVARREIITSSDYRNADWEGTLQ"
     gene            complement(16619..17044)
                     /locus_tag="PK513_03870"
     CDS             complement(16619..17044)
                     /locus_tag="PK513_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81621.1"
                     /translation="MAIMAIEQGLQHTPLIITLLTGAIGVWLYLEWQKYKSLKICCNG
                     YCEALSSAEEKAKLKAKTLKIITEPAMLDGQVVAVAETLDGGAEAVAWDYILKEWVYD
                     PDLNIGEVSCAQHERPLSDHKMDLYGIKYHAKDHGAEHG"
BASE COUNT         5010 a         4861 c         3308 g         3941 t
ORIGIN      
        1 actcggatcg ctattatcct gaataatcgt cggactggca ggatcggcca aggtcaaacg
       61 acagccttca ccttcaatct gcgaggtaat atcgagcaaa tatttcgcgt taaagccaat
      121 ttcaatatcg gtttcgccat tcacctcaac ctcttcggtc gcactgccgg attcggcgct
      181 actcgccgaa actgtcattt gcttgccgtt cagcgcgatc ttgatcgccc ggctcttccc
      241 atcggaaatc gtcgaaacac gatcaaccgc gctggtcagc attttcgcat ccacctcaac
      301 aatcttgtca ttgcctgcag gaataacgcg ctgataatcc gggaacgtcc cgtcaatcag
      361 ctttgaggtc agcacgatat gatcgaacgc aaaccggatc ttactctcag acagcgtaat
      421 ctgaatcgca tccgccgctt catcgaccag cttgcgcagc tcgccaatcg tcttgcgcgg
      481 aataataatg ccaggcattc cgccagcccc ttcgggaagc ggcatctcaa accgcgccag
      541 gcggtgcccg tctgtagcca cggcgcgcaa aaccttcacg ccgtcttggt cagcttcatg
      601 caggtaaatc ccgtttagat aataacgggt ctcctccgtg ctcatcgcaa acttcgtccg
      661 gtcaataagc gcccgcaaat ccgccgccgg aatcgcaaat ccgttcggca aatcaccagc
      721 gccgatttcc gggaagtccg ttgtctgcaa acagctcagc ttaaactgtg aacgtcccgc
      781 cttcacgctc atctgtgtgc cttccgcatt cagtgtaagc tccacatcgc tatcctccgg
      841 caatttacgc acaatgtcgt gcagcgtatg agccggcact gtcgttgcgc ctggctcttt
      901 aatatccgcc gccaccgatt cgtcaatttc cagatccata tcggtcgtcg ccagagacaa
      961 cacaccatcc tccgccttca tcaaaacatt ggaaaggata ggaatagtat tgcggcgctc
     1021 cacaacgctt tggacatgac caagggatcg aagaagggat gcgcgctcaa tgctcagttt
     1081 catagcaaaa tctttcatat taggtacatg atatgggaat cggatactag catagggcct
     1141 taggccgaga aacccccaaa atcatactct taaacgctta tccaaagtga taaaaacccg
     1201 cacggaaaaa cctatgcagc ctgctggaaa caatcattcc gaaaactgtc caaagaccgc
     1261 cacaacgttt catagatgca ctccagatcc tgctgatcta tgcaataagg cggaagaata
     1321 tagacagtat tgccgatcgg acgaagaaga acaccatttg caagcataaa atcataaatc
     1381 tcatgaccga tagaagacaa atacccgttc acatcatcgc gtacatcgat agcaaagatg
     1441 ctgcctttca cccgcacatc cataacatca gggcgcgcgc tataccaccc ggcggcttta
     1501 agatgagaag cctcaatcgc agcaatctgc tccatcaccg gctcttcatc ccaaatctcc
     1561 aaagatgcca gtgccgcaac gcacgccagc ggattgcctg taaaactcgt tgagtgaaaa
     1621 aactgcttgg ccttttcatc atgataaaaa gccttataaa tttcttcggt cgccagcgtc
     1681 gcgcccattg gtagaaaacc gcccgtcagc cccttcgata aacacaaaag atccggtgta
     1741 aaatctgcct gctcacaagc aaacatggaa cccgtacggc caaaacccgt catcacctca
     1801 tcagcgatca aaagcacgcc atgctcacgg cacatctgcg ccatctttcc taaaaccttg
     1861 ggagaataga cgcgcattcc ggccgcacct tgcaccaaag gctcaaacac aaaggctgct
     1921 gtctgcgaac cgaatttctt cagccattta tccagccacg ccaggctgcg catctcctcg
     1981 cctgttttcg gaaacggcac atgcttgacg tcaaacaaaa acggctcgta aagcacatta
     2041 aaaacactgc gagatcccgc tgccatcgcg ccaaatgtat cgccatgata tccgccctcc
     2101 agcgcaatca ccttatgacg ctgctcacct gcattttcat gaaaaccaat cgccattttc
     2161 aacgcaactt ctatcgccgt tgaaccgcta tcggaataaa acacgtaatc cagcgccgaa
     2221 ccgtccttgc cctcaaaagt cccatgcaca gttgaaagca atttacgcgt caacttctcc
     2281 gccggatcat gcgtaaagcc ggcaaaaatc acttgctcca gcttcccggc ctgcgcgcgc
     2341 accgcctcaa caattttcgg atgacaatgc ccgtgcgtat tgacccacca gcttgaaatc
     2401 ccgtcaataa tctcctgccc gccacgcaat ttcaaacgtg caccagccgc agagtccacg
     2461 tgcaaagccg gcccgcaaat cttatgctga gtcataggat gccatatagg tgaaatacgt
     2521 ctcatctaaa aatcacttaa atcaaaattt tgctcgaaag catcagtaag actttttgca
     2581 tccaaagagt caagcattgg aagacgcccc agcactttta cgtctgaaaa ttcaccgatt
     2641 gtacgcatat tatcttcatt ttcatcgcca acaaaaataa ttccatgcac cgggatctta
     2701 cggctccaaa gcgcctcaag cgaaagcaat gtgtggttaa tcgtccccaa gcccgtacgc
     2761 gcaaccaaaa tcaccggcac gcgccctcca ttcacaggat cttcattcca acgtttaaac
     2821 aaattaacca gcaaattctg acgcgtcaaa ggcaccataa gcccacccgc accttcaata
     2881 ataagcggac catcaacctc aggaatcacc aaactctcca catcaatctc cgcctcatcc
     2941 aattccgcag cccgatgcgg agaaagactt tcagtaaaca catagctttc cggcagaaac
     3001 cgctccaacg gcaatcccgt catcttctgc acagcgcgcg tatccacgcc gccttccacg
     3061 cccgattgta caggcttcca gtatgtcgcc ccgagtgcca gcgcgagcat cgcggacaca
     3121 accgtcttcc cgatccccgt atccgtaccc gtgataatgc acctattact catctacgca
     3181 gcctttcgtt ccaaaaaacc atccagatcc gcagcaaatt gatccagccg caccttatcc
     3241 aacgccgcac tcagagaaag tcgcaaccgc gcagtgcctt ctggcaccgt cggcggacga
     3301 atcgcgcgaa tatcatatcc tttttgctgc agtttttgcg caacatccaa cgcatatgca
     3361 tcatcgccca acataatcgg aacaatatgc gttcccggcc cgccaaatga ttgctgcgct
     3421 tttctcgaaa ggcctgcaag ttttttccgt gcaatctccc cctcctctga agcaatccat
     3481 tccagggatt tttgcaccaa atgcacctgt aacggcaacg gcgcggtcga ataaataaac
     3541 ccccgcgcag cattgatcat gaaatcaatt atcgcctcag atccgcacac caagccgcca
     3601 gccacaccga tcgccttacc gcaggtatgc aaaacaatta aattatccgg ccaaacgtca
     3661 ttgcgaggag tatctgcgac gaagcaatct attttttctg gattgcttcg ctgcgctcgc
     3721 aatgacgaat acaattcata cgccagccct ttaccagccc gccccataac gccagtcgca
     3781 tgcgcctcat caacgaccaa aaccgcatca taatcttcag ccaacccata cagcgccttc
     3841 aacggcgcaa tatccccatc catcgaatac acgctctcta ctgcaaccca tatttgacca
     3901 tctttttttt gctgcgcctt tgcttttcta agtgcctctt cgaaagaatt tacatcatta
     3961 tgcacggcct taatatgctg cgcgtttgaa ttctgaatcc cctcacgcgc actcgcatga
     4021 acaaattcat caaaaataat cgtatcatga cgtgtgcaaa gcgtttgaaa caacgcacta
     4081 ttagcctgaa agccagtcgc aaaatacaat gatttcggcg cagaaaaata ccccgccgca
     4141 aaatcttcga gtgccgcatg agcatccgta tgcccgcgca ataatcgcga cccgccagca
     4201 ccgatcaccc cgccattttg taaaaatttg agtgcacatt cccgtaaata cacactactc
     4261 gccaacccca gataatcatt cgaggtgagg tcaattcccg caggcaacat caaagtgcga
     4321 taacgccccc gttgcccgag tcgatccaat gtctgccgaa aatctcgcat catatggttt
     4381 tatagaaaaa accagcgcac ctcaactcca tacttgaaac acacgacaca acgacacttc
     4441 ttaatgactt ttccagactt tcgaaggctt cttgtattca gaagcactat aagactgcgg
     4501 ataaacacgc tttccgcccc tataagacac acctcccctt tttttcgtca ctttattttt
     4561 tggaaaaaca atacaattat tgaaacgcca caaatccaca caatcaagcg ttctacccac
     4621 aggccccgtt gaacacgaac acaacgacaa acacactgcg ctgagcaata aaatctttga
     4681 ccctttaatc ataatacacg ctcccttttt ataattaaac actgttcaat tgctattaca
     4741 gcatcatttt ccatacattg tcaataaaaa attgaacata gttcaaaata tgctattatg
     4801 aacgaagaat catcgtaata aggacaaaca atggcccggc gaaacgacca cacccgtgaa
     4861 gaactcaaat ccctcacatt agaacgcgcc ctcaaaatcg taaaaaatga aggatttcaa
     4921 aagctaacag ccagaagaat agccaaagaa atcaactaca cccccggcac gatctacaac
     4981 atgttcggtt caatggatgg actatatttt gccctcaacg cccacacgtt agacaagctg
     5041 ttcgaaacca tcgccgcccc cgaacaatac cctgccgagg ccacaatttc tgaaaacata
     5101 aaaacaatag ccaaaagcta tatggatttt gcgcaaaata acaaagaact ctggctgatg
     5161 ctcttcaaca tgagctttcc aacaaaagat aaagccccgg attggtacag ggaaaaagtc
     5221 caatccattc tggatatact tgaaaatctg ctggcacccc tcttctcaga aaaccaaacc
     5281 cgcgaaaaag ccatcgcggc gcgcaccata tggtcctcgg tccacggcat atatctgatg
     5341 gaacaaacca ataaaattgg atttatgagc gataaaagcg ccctggatat ggccgaatgc
     5401 ctgatcgata atttcatcgc aggcctgcaa caaaacgcag cctagaatca gaagctaagc
     5461 cgcttctttt tgacacccac aaccaccggg cttaccgcaa ccaccacttt tccgttcggc
     5521 agaatcatcc ttaaatggcg cacgcggctt catccccaat tcatcaagca aagcatggtc
     5581 aaaatctttt tccgggttcg acgtcgtcag caatttctcc cctgtgaaaa tagcattcgc
     5641 cccggcaaaa aacgccagcg cctgcgcttc ttttgaaatt tctaaccgtc cggccgacaa
     5701 ccgcaccatc gatttcggca ttaaaattcg ggccacggca atcgcacgaa tccactcaaa
     5761 aatatccagc ggcagcgcat gttcaagcgg ggttcccttc accggcacca gcatattgat
     5821 tggaacgctt tcaggctgcg gatcaagatt acacagcgta tgcagcaagg aaatccggtc
     5881 ctcttcgctt tcgcccatcc ccaaaattcc gccgcagcac gccgaaatcc ctgctttagc
     5941 cacattgccc agcgtatcaa gccggtcctg ataactgcgc gtcgtgataa ttttatcgta
     6001 aaattcctca gacgtatcca gattgtgatt ataagccttc agccctgcct ctttcagccg
     6061 cttggcttgc tcttccgtga gcatcccaag cgtaacgcaa ctctccatgc ccatgtctga
     6121 tacgcctttg accatgtcac aaacccgatc aaaagcacgc ccctcaagca cacgcggcca
     6181 cgccgcgccc atacaaaagc gggtcgatcc ggcctccttg gccttgcgcg cttcttctag
     6241 cacggcttcg gtcttcatca ggatttcttt ttcaagctcc gcctcattat gcacagactg
     6301 cgaacaatac ccgcaatcct cggtacaccc gccggtcttg atcgacaaga gcgtacacat
     6361 ctgcatctcg taatgatcgt gattggtccg atgcacggtt gcagcatcat aaaccaaatc
     6421 catcaacggg cggtgataga gcgcccgaac ctcttcaaga gtatgtgtca taacgctgaa
     6481 ctctattaca aaaaaaacgc gaggaaaagc gaaatccccc gcgctttaaa caattcaaaa
     6541 ctcgccttac acaaaaaaca atcagccagt aagagcacgg cgcacaacat caacatcctg
     6601 tgcgatttgt gcatcttctt caatcagctc ctcaaccttg cgcaccgcat gcatcacggt
     6661 ggtgtggtca cgcccaccaa atttacggcc gatttctggc aaactccgcg ccgtaagctg
     6721 cttcgcaaga tacatcgcca cctgacgagg ccgcgccaca ttccgcgccc ggcgcgccga
     6781 atgcatatcg gccattcgaa gattgtaatg ctcggccact ttacgctgga tctcatcaat
     6841 cgtaatgcgc cgatcatgtg cacgaagtaa atcttgcaaa acttcctgcg tgctttccag
     6901 cgtcacttcc gccttcgtca catcggcatg cgcgacaatc cggttcagcg caccctcaag
     6961 ttcacgaata ttgctggtca ctttcagcgc cagaaattca agaacactat caggcacatc
     7021 cgcgccaagc tgcgcgcgtt tggtttgcaa aatcccaagc cgcaaatcat aggtcgtcgg
     7081 atgaatatcc gccaccagac cccaggccag acgtgaacgc aaacgctcat ccagaccgga
     7141 aagatcgctt ggcgctttat cagcagaaat aataatctgc ttattctgat ccacaagcgc
     7201 attgaacgta tgaaaaaatt cttcctgcgt tgcttccttg cccgcaataa attgaatatc
     7261 atcaatcatc aatacatcaa cactgcggaa tttctcctta aacatcatcg tcgaatcact
     7321 gcgcaaagct ttcacaaact gatacataaa tttctcagcc gaaagataca tcacggtttt
     7381 ctcaggactc tgctcttcaa tcgcatgcgc aatcgcgtgc ataaggtgcg tttttcccaa
     7441 gccaaccccg ccatagataa acagcggatt aaacggaatc gtcaagcttt ccaccacccg
     7501 ccgcgccgcc gcatgcgcca gcgcattagg cttgccaaca acaaatgttt caaacgtaaa
     7561 acgctcatcc agcggcgagg atatttcaga aatatccagc ttcttgttct ggttggcttt
     7621 ggaaggcgcg gccgtttcag gctcatcatc cacaatcgca ttttgcacaa cgacaatctg
     7681 caaacgcttc acgccatctt caaacccttc gctggcttca atgcacatgc ccaaaatccg
     7741 ctcagcatag tgattctgta tccagtcacg cataaaccgt gtggggacgg aaacttccat
     7801 cgtgccatga taaaaagcct ggagagtaag aggcttaagc cagctgcgga agattgcttc
     7861 accaaattca ctgcgcatat cattatgcac ctttttccag agcttcataa tttcaacgct
     7921 cggctccaaa gatgcttcct gatactcagc attgccccga atcaaagcaa cggactttcc
     7981 acccatgtct gacatcttct attctctcca tttgtcaaag gttacatatt aaacaaaaca
     8041 aaatatacaa tcgtcaaagc gccaaaaaag gcagcaaaaa ggggtatacg aaagccgaaa
     8101 aaggcccgca aacacgtttc ctataacgat taaactttta aattatgagt tccgaaccca
     8161 actccaagcc aaaagcagca atccttgcca caaacaactc aacggcccct atccccaaaa
     8221 ccctcttaat aaaaagaagg caaacttcaa atcagaacaa aaccctactc aaacacaaca
     8281 cacacaaaag catgctgcaa aaaacttcct caagaccaat tcggcctctt ttttgtcccg
     8341 atagaaataa gcctacacta aaaaaatatg tggatgcaat acgaacaaaa caagaaaatc
     8401 aaaaaagaaa tttttttata tttttttata ctattgattt taaacacatt taagcccttc
     8461 agattttcat ctcaagaact taaaaacaat ctataaaatt caaatattta gtatgaaacg
     8521 ctactaaact ttcgcgtttt tagcagtagc taagccgctt ttttaagcag cttaatacga
     8581 gaacttaatc tggaaacctt acgagccgcc gtattcttat gcaaaatacc cttaacgact
     8641 ccacgactga tttctggctg cgcattttgc aacgcactct cagcttcttt tgcatcgcca
     8701 gccaccagag ctaactcaac ttttttaata aatgtacgaa tgcggctcat gcgtgcacca
     8761 ttgatagcgg cacgcttatc gttccggcga attcttttct ttgaagattt atggtttgcc
     8821 attgttaaac ctttattcct aatttcttct caaaagcgct gacttatcga cttttgtgga
     8881 agccgccagc gcaccttatc cacagattgg gagaggtata cgctgctcca gcccggcttg
     8941 tcaaggaaat gatacgtttt ttattagcct aaaaatcata tgctagaaaa tgatcaaact
     9001 aagacgcaaa aaccagcttt cacaccccgc gaatcggcga gcctatttcg gttgaatgct
     9061 caaacgcaat ataataatac cggcctcatc ctcaaacatc atttccaaac gctccccatt
     9121 acgaatgaat tgatctccac caaccggatt ccaaccgaat tgcggcaaaa ccgatgcata
     9181 ataagcccgc accgaatcaa cgctcattgc cgccatctcg gcaactgatt caaccacgcg
     9241 cccaccaggc ttatcaaagc tcatcgagcg ctcaggcagc tctatcaaac ccggcatcaa
     9301 cggcacatcg tcgagcacct caaaaaactg aaccgaatca tcctgcgcat atacagaagc
     9361 agaggccata accatccctg ctaaaatcat caccgcaata ataacgaaaa tacgcatacg
     9421 ctctaaacct taaccctgtt tttcaggaca aaaaaatgtt gaacggccag cttgtacaat
     9481 ccgctgcaca ccacccgttt ccactatatc acactcacaa gctccgcagc cttgcccaac
     9541 ccgatcatac acagcaaaat gatgctgaaa ataccctaac tgaccatccg tatgcttgta
     9601 atcctttaac gacgaaccac cggcctcaat cgcatcgcgt aaaaccgaac gaatagacat
     9661 gcacaacgcc gtacactcct ttgcgttcaa atcaccagcc ttacgccgcg gatcgattgc
     9721 cgcgcggtaa agcgcctcgc atacataaat attgcccagc cccgccacaa tccgttgatc
     9781 cagcaatgcc gatttaattg gagtctttcg cccctttatc gtcaccgcta aaatctcagc
     9841 attaaaatca ttccccagcg gctccggccc catagctgcc aaagccgggt aatcctgaca
     9901 atcctcacgc ccgaccgcat caagaaaacc aaagcgccgc ggatcgttca agacgacgcg
     9961 cagcccctca tccatataaa actcgacatg atcgtgtttt tcgtgaagag agatgctccc
    10021 cggcggctca atccgaaccg ctccgctcat ccccagatgc aggacaaacc cggcgccatt
    10081 ctcggcaaac cctagaatat acttcccccg gcgcagcagg cactcaatcc tctgcccttg
    10141 caacgctgcg ggtaagcctt gcggcaaagg aaaccgcaaa tccgcgcggt taaaaaccaa
    10201 ccgcttgacg cgccgcccca ccatcgcagg ctccagcgcc cgcttcacaa tctcaacttc
    10261 cggcaattca ggcataacaa cagcaatcta gcgcaccagg ccgccaaagc aacgtcaaaa
    10321 gcgaaaacac tatagtcaaa cagattggtt ttttgacgag gcgcaaggag cgacgtgtac
    10381 aaattgctac acgagcgacg cagcaacaca atcaaaaaac caatctgttt gactataaaa
    10441 gaaaagccgg ccagtttggg gaaactgacc ggcgagagag tttacgtgtg tggggataaa
    10501 agctcgacct ccatacgcat caggagagaa accttatttt tcatacagat tatcaagctt
    10561 tgtaccctat ttatagcaac tgaaaccacc cgtcaagaaa aaagttataa aaactgcata
    10621 aaaagatttt ttttataaca aaaaccgttt tacatatgaa aaaaggactg gacaaagcta
    10681 taaaaggaga ctatagacta ggcatcatta taaattaggc gtggattacc cgcatattaa
    10741 ggataggaag acgatgccga cgattgaaca aatcagagcc gcccgggccc ttttaggatg
    10801 gaaccagcac gagttggccg acaaggccgg cctgtcgcaa accggcatcg cgcgcatcga
    10861 aaacggcacc aaccagccga actctaaaac cctggaaaaa atcggcaccg ccttcgacca
    10921 cgccgatatc gaattcatcg gcgacaccgg cctgagaaaa cgcagcattc aatcacaaac
    10981 atatcgtggc attgaaggct ttaaagaatt tctggataat gtttatcaca ctgcgcgtga
    11041 tgttggcggt gaactctgca cttttaacgc cgcaacacca tactttaaaa caatgcttgg
    11101 cgcagaatgg tatgaacaac acgctgaacg catgcaagcc attcgagaca acttcaccat
    11161 gcgcctgatc gttgaagaag gaacgaaaga cctactcgca aagggctttg gagaatacaa
    11221 attctttcca aaaaatctgc taacagatca aaaaatctac gtctatggcg attttgtaag
    11281 ctttatgaat tttgccaatg aaaacgttga aatcgttgag gttaaacatg gccccagcgc
    11341 agaaagtttt cgaattttgt ttaacattgc gtgggagaag gttgcaacat ccctcaaaga
    11401 gtaatgcgca ctaaaaaacg aacattgata atggcagaga agaattcatg tttttcattc
    11461 aatgtcgtat attgtctgag taatttatta tgagcgaaag cgacaacaca gaaacatctc
    11521 cagtgccaga aggcgcaaaa agcgcttttg ccgcagcgaa tggcacaaac ggacacggac
    11581 aaataaacgt tgccatcatc ggcgctggct ctgttggtgt cttcacggct attgaagtgg
    11641 caaaaaacgg cgcacgcgta tctctcgtct cgcgtcccgg ttcccaaacg ctacaagccc
    11701 tgaaacaaca gaaatatatt gagctggcct ctacaaaaac aacacgcgaa attgacgcca
    11761 tgtttgaagc caaaggcaat tacatctccc cacctgctga aatttccgat attccacaag
    11821 atgacgactt aacagaagtt ataggagata aacgcgttat ttctcacgga aaattagctg
    11881 ataaattaaa gaaaatgggt aacgccgtcg gcaacgaact ttttggcgcc gtaaatgctc
    11941 aaactcaaaa agaatatttc acgcaaggtg aaatccacac gcttctaacc aaacacctgc
    12001 gcataccgct ttacgtgcaa gaaagagatg gagacgaaga agcatataga aaagcactcg
    12061 gaagcacccg ctacgtaaag ctttttgata atataaagga tgccgaacct gaaaatcagg
    12121 tcataattgt ggcaaccaaa agctttagca aaagtgcaga gtttgccaaa agaaccgtac
    12181 ttccgttttt agctgaaaat ggcgtggttg ttgacctttc caacggatta aaacgctctt
    12241 ctattccaaa actagaacga acaaacctga gacaaaccct gcatgaggct ttaacggaaa
    12301 gcgatgtcct cgccgactta aacgcttttg aagacgaaat tggcccagaa agaatcatcg
    12361 gcgccaacgc cttattcgca gtcgaggcag aaaaaaacag ccctcacaga gtgcatgtca
    12421 gatctctgct acacaccgct gctatatata cagcaaacac caatccttac gtcgaacgga
    12481 tttttgaagg cagtgacatc aggccgtcca caccgcacga ccctctggcc caaagtctgg
    12541 acaaactcgg catcaacatg atgaatatca tgagcgctgt ctacgacaaa tcaaaagcag
    12601 aagttttcag caatcagacc ttactaaacc tttataacaa cgcgttaaga gaactatacc
    12661 gggtagcgga aacaggatat ggggtcacac tccacagagg cggccccgaa gcctatattg
    12721 aacactgcca aagataccac agaagagcag ttctcgccgc ttcacacgcg ggagatatac
    12781 accgctcctc aacacataaa gacattgcag aaggaaaagc aaaaacagaa agagagtttt
    12841 tactcgatgc catcattgaa ctttctgaca agcagaaaat ccaagtgcct caccttcgca
    12901 ttctctcatc ttttatggac acaattgagg aattaatcaa agaaaaccaa ttggatgaat
    12961 tgcgcaaaca acactacatt ttctacgaaa acacccacga aatgagggat ggaacggtga
    13021 cgcgcgtaca cccgccaaaa accagtaaag acgttgagct tccgaatgta ggaactgttg
    13081 tcacctcctg caaaatgccc ttttttgaat ggagaagact tcacaataat cgccccgact
    13141 attttagaaa tattatgccg caacttttgc acgctgtaga ccccaaccac gccgtcttta
    13201 aaaacggaaa atgcgtagag ctgatgcaag ccttcgtgga tagcaaagac gttaatcccg
    13261 agacaggaga aatcaacctc gaaagttcgg cattcaacgt tttctgggaa acaattaaaa
    13321 cctacaaaga gcagtgggat gcctacactg cggcaaaaaa gaatgacgaa aaacccgaag
    13381 aaaaacccga agcaaagcgc aaacgcgttg aactggcgtg gcaagcggtc aatgatgcaa
    13441 aaaacgaact ggaacaacgg tacagaaacg ccagaacgct tacaaacaac agaggtggcg
    13501 ctggaaccgg cggagcaaca acgattaccg cagactttac gcacgcaaac gacgacgcca
    13561 acccatggct taataaaaaa agaggggtca gaaaaattct acccaaatat tctactttcg
    13621 atggcccata ttctactttc gatggccccg ggtgcttcta aagctccttt aaaccctaat
    13681 aaacaaagca taaaaaaggc ctccgcattc acgaaggcct ctttctttaa aaaatcgcca
    13741 ggtaaaaaag gcagcacttt ttacaaagaa ttttgccgtt aggctagaaa gatgaggcga
    13801 cgtttattct tataaacgag ccgaaatctg acgctgtcct aacggcaata attcaaagta
    13861 aaattacatc atgccggaca taccgcccat accacccatg ccgcccatat cacccatacc
    13921 ggcaccgcct ttgtcctctt gcggagcttc ggtgatgatc gcttcggtgg taatcagcag
    13981 gctggaaaca gacgcggcat cctgcagggc tgtgcggaca accttcacag ggtcgatgat
    14041 cccggctttg accagatcac agaattcacc ggcctgagca tcataaccgt aatttgtatc
    14101 tttttgctcc agcagcttgc caacaatgac agagccttcc gcgcctgcat tctgggcaat
    14161 ctgacggatc ggcgcttgca aggcccggcg aatgatttca ataccaaccg tctggtcgtc
    14221 attgtcacct tcgagcgcct caagagcctt cgtcgcataa agaagcgcag taccgccacc
    14281 agcgataatg ccttcttcca cagctgcgcg cgtcgcatga agcgcatcgt caacgcggtc
    14341 tttgcgttct ttcacttcaa cttcggtcac accgccaacg cgaagaaccg cgaccccgcc
    14401 tgagagtttc gccaaacgct cttgcagctt ctctttatcg taatcggaat cagttgcttc
    14461 aacctgcgtc cggatttgcg cacagcgggc atcaatgtcg cccttgtcgc ccgccccatc
    14521 aacgactgtt gtatcgtctt tggtgataga cactttcttg gcgctgccca gcatatccag
    14581 cgtaacattt tccagcttga tacccagatc ctcggaaatg acctgaccac cagtcagaat
    14641 cgccatatct tcaagcatcg ccttacgacg atcgccaaaa ccaggcgctt tcacagctgc
    14701 aattttcaag ccgccgcgca acttgttcac aaccagagtt gccaaagctt cgccttcaac
    14761 gtcttcggct acaatcaaca atggacgacc ggattgaaca accgcttcca aaatcggtac
    14821 caaagcctgc aggttcgaaa gcttgccctc gttcagcaag atgtatggat tctcaagatt
    14881 agcctgcatc ttgtccgcat cagtgatgaa gtatggggac agataaccgc gatcaaactg
    14941 cataccttca acaacttcaa gttcgttgtc caaagacttg gcttcttcaa ccgtgatcac
    15001 gccttcattg cccactttgt ccatcgcttc ggccaagagc gcaccaattg actcttcacc
    15061 attggcagaa atcgtaccaa cctgcttgat ctcttcatta cctttgacgt tcttcgcacg
    15121 cttggcgaga tcttcaacaa ccttggcaac cgccatatca atcccgcgtt tcagatccat
    15181 cggattacga cccgcagaaa cagcttttgc gccttcatta gcgattgact gtgccagcac
    15241 cgtcgccgtc gtcgtaccgt caccggcatg atcattagcc ttggatgcca cttctttgat
    15301 caactgcgca ccggtattct gacgcttgtt ggaaagttcg atttctttcg caacagtcac
    15361 gccgtctttt gttgtacgcg gcgcaccgaa cgatttttca atcacaacat tgcgaccctt
    15421 cggacccaat gtcactttca cagcattggc caaaacatta atcccggcca acatatcttc
    15481 acgggcatct ccgcccagct ttacatcttt tgcagacatt ataactactc ctttaaatct
    15541 acattctttg ttaattttca ggtccccgtc tacgcgggga cgccttgtgg cgattaagca
    15601 gcatccacga tgccgatgat gtcagactct ttcatcacca tcagctcttc accatcaatg
    15661 gtcacttccg taccggccca ttttgaaaag atgacattgt caccctcttt cacatctaac
    15721 ggacgcacat cgccattgtc attcacatgg ccagagccaa cagcaacgat ttcaccttcc
    15781 ataggctttt cagatgctgt gtcagggatg ataatcccgc cagccgtttt ggtatccgct
    15841 tcaacgcggc ggacaagaac gcgatcatgt agaggacgaa acttagccat ttcgtatgtc
    15901 tccttctttt taagttgata acaagcagga ttttgatctg cctcatatga agccaggttc
    15961 aaaaccccct ccaacgctcc actaagcctt atttaaggct catgtttgcg acaagcatca
    16021 aacatatagg ccaccgctca aaaaaattca agtttttttg tacacaatgc aaaaccaata
    16081 gaaaatgttg ataattaagg cttttgcaag attttttgct taaaataaag tattatagaa
    16141 acagaacgtt tcgatacgcg caaggaatga gtgagggagc aaatggctga tttaactaaa
    16201 caattctgca attaccgcct gacaacagca aaaattctat accatatgcc ggattatgaa
    16261 cggatcttgc aggaatttat ctggcaggaa tacgaccttg cgccccaatt tccagaacta
    16321 tttaaatttc ttgatttctg ggagaaaaag atcgaaggtc gcctgcactc ggtctatgtc
    16381 gcgcggcgcg aaatcatcac cagctccgac taccgcaacg ccgattggga aggcacactg
    16441 caataacgaa cacctctttg ctttcccgcg gcaactaggc catcaaaaat atatcggggc
    16501 tctacatgac gttctcaatg aacagggctt taatctggtc aagagtctct cactcatttg
    16561 cgcaaatata aaaaacgctg catacacaac cagcaataaa atcgctgcaa taattttctt
    16621 agccatgctc tgctccatga tcttttgcgt gatatttaat tccgtataaa tccattttat
    16681 ggtctgaaag cggtctttcg tgctgagcac aagaaacctc tccgatattg aggtcagggt
    16741 cgtaaaccca ttcttttagg atataatccc aggcaacggc ttcagcacca ccgtcaagcg
    16801 tttctgcaac agcaacaacc tggccatcta acatggctgg ttctgttatg atttttaggg
    16861 tttttgcttt caattttgcc ttttcttcgg cagaggagag tgcctcacag taaccattac
    16921 agcatatttt tagagatttg tatttttgcc attcaagata tagccaaaca ccaatagcgc
    16981 ctgtcaggag cgtaataatc aagggcgtgt gctgtaggcc ttgctctatc gccattatag
    17041 ccataccaat tcccaaaaag gccagtaact cacgcctatc gtgtcagatt atcatggatg
    17101 aaagtctgga tgggttgaag
//
LOCUS       DAOSWR010000032        16808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2152_length_16808_cov_5.182952, whole genome shotgun sequence.
ACCESSION   DAOSWR010000032 DAOSWR010000000
VERSION     DAOSWR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_16808_cov_5.182952"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..282
                     /locus_tag="PK513_03875"
     tRNA            206..282
                     /locus_tag="PK513_03875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Met,seq:cat)
     gene            376..2670
                     /locus_tag="PK513_03880"
     CDS             376..2670
                     /locus_tag="PK513_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO81622.1"
                     /translation="MLEKLISIKGVGRFQNCKAAGDVSFGKNSLVFAENGRGKTTLCA
                     ILRSLQTGMPAYIMGRQTIGGEENPPEVQIRLNGGNAVFRNGQWNQQVSDIAIFDSTF
                     VAENVFSGDCVEIDHRRNLYNVIIGKAGVDLALEVNAIDAAIKIKNGEIKDKKAFLES
                     RIPDGVMDADYFITLPKEDDSIDSKVADKERDLQAVKQSAQLQRGSELEQMSLPAVPD
                     EFLPILTKTIDGVEADAERQITAHIETHNMQARGKTWLSEGCSYIHDDNCPFCGQDLT
                     GVDLIEAYKGFFSAAYNALKDEIKALSDEISDSFGDLGLAQIDRVQHKNAAAVEFWSQ
                     YCDITAPALQESYDLIVVLARLRQSALTHLERKAQAPLEQINPDASLLSDFEILRQFQ
                     AAIVQYNQAVDTANALIRAKKEQAQGREVRQVETELAKLKAAQRRCDQQIDAECTAYR
                     KLLMEKKDLEDRKTAAKEKLDEYTQSIIKKYEKTINEYLDCFNAGFQITETSHGYRGG
                     APSTSYQILINQTPVDLGNSDTPIDQPSFKNTLSSGDRSALALSFFLAQLEHDPDKAN
                     KIVVFDDPFNSQDDFRKGHTAQRIKKCGRECAQVIVLSHDQHFLKLVWDDLPTDERKT
                     FQLFRISEKNTGIAEWDIEKAVQARYRADIDTLSKYYTSANDGNQRDVVQKIRPVLEG
                     YCRNLYPTRFADNDALGTIVGKIRGYDDEFPLKDVCDELEELNGYSRRYHHGENPNAA
                     TEAINDNELKGYVKRTLKVVGALL"
     gene            2859..3068
                     /locus_tag="PK513_03885"
     CDS             2859..3068
                     /locus_tag="PK513_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81623.1"
                     /translation="MDSGPDNSAYPGCCDMACGGCGMHHHHHAANGLSDGFSLQAATK
                     DRQGFGAESIYLSDFVYGLKRPPRI"
     gene            3207..3479
                     /locus_tag="PK513_03890"
     CDS             3207..3479
                     /locus_tag="PK513_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81624.1"
                     /translation="MKSLLTFTFIALLGVSGVAKADQNAETIAVEDMPRFASIEQQRC
                     PKSRYPSVKKRLECKHEVRREIFEKRQAREQQNQSAAQSENQGEIQ"
     gene            3476..4738
                     /locus_tag="PK513_03895"
     CDS             3476..4738
                     /locus_tag="PK513_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO81625.1"
                     /translation="MIQHLKILAITALVMPFMFGIAQAQELASKKPGIENFINYIWQA
                     SPSVQEAEARVAAAQAGQKAASRWQHNPEVEYEVEDVEGEEKTQVLGVSQTIDWSGKF
                     LSSGKTAKFELMAAQAERDEIRQNIAIDVLAALAEYNATKDVFGLATQRSSLMERFAG
                     LADKSFKTGDIDQSEYNLAQLALSEALIEQADSETALAESRQALDAAIGFAPDNFFAL
                     PELPETLPEPVLGIDNEDQVLMQLPSLRARKHREEAAKAVITQAQKDRLPDPTVGLVG
                     GKDAGADMIGFSVSMPLYVFNTYGAEVEQAKQEALAEAKAGQNAYHAARTRLQASRSA
                     YQLSRKAWENWQENGAQALAEQVDILDRKVKVGDLSATDYLVQIEQALDTEVAAKELH
                     RKVWQSWFAWLAASGSVEQWIQNTGENQ"
     gene            4735..5979
                     /locus_tag="PK513_03900"
     CDS             4735..5979
                     /locus_tag="PK513_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO81626.1"
                     /translation="MRLLLVLTILAAFSFTFYSYAAEDGHGHDEGSHVEKEQDEPGAG
                     HEESGEHEDHGEKESEEGHGDHGEEGGHGHEEKSEIEISPESLKAAGILVEPLTLQNL
                     EGEIQAPGEVVLNSYLSSKVTPRIEAQIVSRHAKLGDKVEQGTPLVTLSSVEMAAAIG
                     ESLVAHKEWRRVQQLGSAAVSAKRYSEAQIADEQARAKVMAYGMTEQQIEALHKNGAK
                     GNPGEFQLLAPQAGTIVSDNFIEGELIEPGRVLFDIVNESALWVESRLSPENAKQIET
                     EANARIHVPGNGWLQGKVVQKHHLLDEETRTIGIRIEVDNEEDRLHPGMYVDTAIQTG
                     GEQKYLAVPTSSVLRSADGDWVVYVEEKPGQFKPQEVQTLRTVEDYTVIDGLPEGTRV
                     VTEGAFFVQSELAKSGFEVHNH"
     gene            5995..9144
                     /locus_tag="PK513_03905"
     CDS             5995..9144
                     /locus_tag="PK513_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004588883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOO81627.1"
                     /translation="MLNTIIDGAVRNRLMVILMLASILVGAIMILPNLNLDAFPDVTN
                     VQVTVNTEAEGLAAEEVEQLITYPVEAVMYSLPDVEEVRSISKTGLSIVTVVFTEGTD
                     IYFARQLVFERLQDAREQIPEGVGTPEMGPNTSGLGQVFQYMLRTNDPDTFDIMALRS
                     LNDWVVKLLLMPIDGVTEVLSFGGEVKQYQVNLEPAKLLSYQLHAEDVVEAIEENNRN
                     AGGWYMNKGSEQLVIRGVGWVRGGEDGLRDIANVPVKDEDGVVVKVSDVAEVSYGSEI
                     RQGAVSITMRDESGKPEQMGEVVTGVVMKRMGANTKNTIDQIYERLPIIQNALPEGVI
                     IEPFYDQADLIGKAVHTVSKALIEAFVLIVIVLFLFLMNIRAAILVLLSVPISIAMAL
                     MAMAYWGISANLMSLGGLAIAIGMMVDGSVVMMENIFKHLSHHDESDNPQGTSLRILE
                     AAREVGRPVFFAVLIIIVVFTPLFTLEGVEGKLFQPMAVSIVLAMLASLMVALVVIPA
                     LATYLFRRSVDHKSNKLIDAIDAGYKKSLSAALKAKKAIVITAVALFIGSMALVPFLG
                     TEFVPELEEGTVNIRVTLAPSSSLETSLEVAQTLERKLMEFPEVLYASSRVGRAELGG
                     DPEPVSNVEIYVGLKPVKEWTSASNRFELQRLMEEKMSVHPGLLFTFSQPIATRVDEL
                     LSGVKAQLAIKLFGPDLNILAEKGKAIETLVRKIEGAQSVEMEQIAGEAQLVIKPDRD
                     QLARYGIPVGQVMSLVSDAIGGNEAGQVIQGNERYDIYVRLAKKYRNDPHAIADLIMQ
                     APSGAWVRIGDVADVSIEAGPPQIRRDDVQRRVVIQSNLTGRDMGGFVEEINKRIKEE
                     VKLPPGYSVVFGGQFENQKRAQARLMIVVPLSLGLIFLLLFFAFGSVRQAALIMLNVP
                     LAMIGGIAALYFSGQYLSVPGSIGFIALFGVAVLNGVVMVNAINLLIEGGTDADTAVF
                     SGALSRLRPVLMTASIAALGLIPMLLATGIGSEVQKPLATVVVGGLFSSTLLTLFVLP
                     VLYGVFSRRFVEEHKRKVS"
     gene            9160..9408
                     /locus_tag="PK513_03910"
     CDS             9160..9408
                     /locus_tag="PK513_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81628.1"
                     /translation="MKKTIPILIACLALAGCESFRAPTVEQRLAGKTGAERQQELYYI
                     CLERFGDPIYGGGHYPGHEAHREALCHHMDDLNKETEE"
     gene            9411..9665
                     /locus_tag="PK513_03915"
     CDS             9411..9665
                     /locus_tag="PK513_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81629.1"
                     /translation="MPKKTLLLLPFVAVLSACSWSHYGMLEKKLDGKTEDEKRLILAE
                     ECKKEIERGLVKDNPKNIEQHKRMQYVCEEMTGRPVEVRY"
     gene            9668..10543
                     /locus_tag="PK513_03920"
     CDS             9668..10543
                     /locus_tag="PK513_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO81630.1"
                     /translation="MGHHHHHGNPSEIGERRLWWAVLANMLLTVAQVIGGIISGSLSL
                     VADALHNFSDAASLLIALVAIRIGRKPPDQFKTFGYKRAETVAALINLTTLIVIGLYL
                     CYEAVTRFITPQEIAGWTVIAVAGIALVVDIFTAVLTYNQSKTSMNIKAVFLHNLTDA
                     LASVGVIITGTLILLFGWVWTDAAMTLIIAGYVLWQGFTEIPKTIHLLMEGTPEGMDI
                     KEVVAAMESEKGVVNVHHVHVWQLDEHRNALEAHVVLSESAEMDDLKTRLKKILHDKF
                     DIEHSTLEFENTVCN"
     gene            complement(10585..11343)
                     /locus_tag="PK513_03925"
     CDS             complement(10585..11343)
                     /locus_tag="PK513_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOO81631.1"
                     /translation="MFTVLLKNVLGKMPMPEPEVPENRHIRLVEDEPQTEIEAEEQAE
                     ALADSISTAVFITYKNSKGEESERRISVKRVSRSAKGDLLVRAYCFERKAVRCFRADR
                     IVEAVDLATGEVWDTQTEILECFGVVEKTLPVDPKAATREALTKCRHAMNVLVYLARC
                     DGHFHHEEENIIIHYLMDECFDCEFHDEYLLARIRKLYPDTDSFFDSLDYLEEKNPEA
                     LGKLARYAARLVQADGEIAKDEMDFMNELQAYST"
     gene            11888..12139
                     /locus_tag="PK513_03930"
     CDS             11888..12139
                     /locus_tag="PK513_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81632.1"
                     /translation="MKGAVMSLSSFKQTLENRLYGDIWGAAKPEEAAGVLVSVYGGRA
                     INEAFERETEAWKQSDFSRAVFWACVLEKLSPDFLPCQH"
     gene            12150..12641
                     /locus_tag="PK513_03935"
     CDS             12150..12641
                     /locus_tag="PK513_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81633.1"
                     /translation="MAKSKNTGPRATPIDIHVGKRIRIRRCLLGISQQRLGSSINKTF
                     QQVQKYEEGSNRVAAGVLYDVSKALDVPFDYFVYGYEESQIQKTAGLSDNEQEPFLHV
                     LEDSRKKLNAALTVENLSTNGALICFIRKYTEAVLLNHHNVQKVNKFLDELIEKEKER
                     GGE"
     gene            12656..12934
                     /locus_tag="PK513_03940"
     CDS             12656..12934
                     /locus_tag="PK513_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81634.1"
                     /translation="MTLLLLAGCTNYSAFERKVIERAYSEPATAPEYFMHQIESGATV
                     EEQAVYMYGIGLAHEKLGNKAEALGDYLSAEALGYNKAAEALERLKTK"
     gene            complement(12927..13688)
                     /locus_tag="PK513_03945"
     CDS             complement(12927..13688)
                     /locus_tag="PK513_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81635.1"
                     /translation="MLVNTGNMFAYGRRERDPILSIGNLYHYSDPALSKKVFHSENYL
                     EEIHPIKGTYILEQPAPESAMHFNPAFFNGLGGYGFEDVARWMMYDADPTPFQVERTI
                     GMVIDMPGPFENNQIFGFSKFNAFNTHWRLSTSGASGGRITYGRAEDHPDGEFPSLVT
                     GALGASYIIIIRQVSLTQHEFYINSPDNPVILNPNDGYWTFDRIRLMLGRIGGTRSET
                     GVRIGPIFDAFCLISPGHLRKIMEFWSARTGIPLT"
     gene            complement(13690..13878)
                     /locus_tag="PK513_03950"
     CDS             complement(13690..13878)
                     /locus_tag="PK513_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81636.1"
                     /translation="MIKPEIPPSLRQCQTSPAYPGDEVTQKDIAAWGAKLWYAHQDCR
                     SKLENINKALGYTTTKDN"
     gene            complement(13871..14236)
                     /locus_tag="PK513_03955"
     CDS             complement(13871..14236)
                     /locus_tag="PK513_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81637.1"
                     /translation="MISFILKNWKWLSAVFTALILVGYIAWQDHEITNLERKLLASQA
                     SAASYEKSLAELQTDTKAKIEALENENNQSVTRAKELERLLGTIEGADDEKDGPCAPV
                     LCDTIKRLYGKPADTSKSD"
     gene            complement(14233..14463)
                     /locus_tag="PK513_03960"
     CDS             complement(14233..14463)
                     /locus_tag="PK513_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81638.1"
                     /translation="MIKPDNTDPIRVRRFLAFWVSVYAFLILPNLLVAYQILAGADIG
                     LLQSILTYIGILASGPIGAYLYAAHKNGNKEK"
     gene            complement(14456..14941)
                     /locus_tag="PK513_03965"
     CDS             complement(14456..14941)
                     /locus_tag="PK513_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002051885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81639.1"
                     /translation="MDIRQLRDYVVRPALERINAWTPNAEQLVIGTALAESRAFFIHQ
                     VGRGPARGFFQMEPVTHDDIWERYLSRRPALLNELKGLIMRDMDLHDQLHGNLFYAAA
                     MCRIFYLRFKEPLPEMNDWPGMAAYWKKYYNTPAGAGTVEGFIQKARPAFDLYVGGIN
                     D"
     gene            complement(14941..15219)
                     /locus_tag="PK513_03970"
     CDS             complement(14941..15219)
                     /locus_tag="PK513_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81640.1"
                     /translation="MTPAEWGLLFGIIAHSVAVIMALVKLVVWVTSHVTASEQKIQIL
                     EQQVNNDVTGRRVVGEMKEDLATIKAQIKDMREDMHHLRKKVDENGGQ"
     gene            complement(15241..>16808)
                     /locus_tag="PK513_03975"
     CDS             complement(15241..>16808)
                     /locus_tag="PK513_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOO81641.1"
                     /translation="YLAAREFPRPEDYGAKRNGIDDDAPAINQCLQENKLAILSPGTY
                     RIAEPIVLNYGMSILGYGDMSIIQAQEEALNENELPPYSADFNAIEIVEGYCLVQDLK
                     IIGGASALFLAGKVGPCVKNVIENVTIWDAQIGVTLDGWNDTQKPCYWNNISRVLVAR
                     PGINGVLLTTTPDDWEDPETFGDSPNANKFYDVRVYSLSSPLSGSGFFISTGQYNNSF
                     IDCEANVHTDAEACFRLGFLARHNVITNFYAECLGLAPGIRIDNGSIDNTIIRLFSAT
                     GGAAIWDPTLQGEYQAFDAGYPKKNFIKDAYITDVHIDGMTRGTTYIDPPEDHVGDIS
                     IDLEHTFYLLSSFNKSYDLILPNAGNATGRWVTLKKSDLRDNPIYVKEDSGNGPDNRT
                     LTLLQQYDTVTLVSNGATWWICQDNRMPRPSRYIDNAAAPSGTFQADMLNRVYLVSAF
                     SGQIIFEIPTPAQAAGRMCTIKKIDPSGNHVKVQMAGGSGGPDAQIWSLTSHFAAITI
                     YSDGGQWIVISTY"
BASE COUNT         4510 a         3773 c         4198 g         4327 t
ORIGIN      
        1 tgtatcgcgg gatggagcag cccggtagct cgtcaggctc ataacctaac gagttgaaaa
       61 aatggacgga ttaagaggtt gaaaagtata ggagttttta agagcgtaaa aagttaaaaa
      121 gtgctatttg tagcagtaag tagcagtgaa atgtcatacg ggattgtcat acgcttttat
      181 gttatatatg aattgatttt tttgacgcgg ggtggagcag tctggtagct cgttaggctc
      241 ataacctaaa ggtcagtggt tcaaatccac tccccgcaac cagttttaaa taaagccgct
      301 tcatagcggc tttatttttg tccattttaa taatattgtt aatggcaatc agaaaatttt
      361 taaaaggatg cggggatgct tgagaagctc atatcaataa agggcgtggg ccggtttcaa
      421 aattgtaagg ctgccggtga cgtaagcttt ggcaaaaaca gcctagtgtt tgccgaaaat
      481 ggccggggca agacaaccct ttgcgctata ttgcgctcac ttcaaaccgg catgcccgcc
      541 tatattatgg ggcggcaaac cataggtggc gaagaaaacc cgcccgaggt tcaaatccgg
      601 cttaacggcg gtaatgccgt cttcaggaac ggacaatgga accagcaagt ttccgatatt
      661 gctattttcg attcaacctt tgtggccgaa aatgtttttt ccggcgattg cgttgaaata
      721 gaccatagaa gaaacttgta taatgtcatc ataggcaagg cgggcgttga tctggccctt
      781 gaagtcaacg ccattgacgc ggctatcaag atcaaaaacg gtgaaatcaa agataagaaa
      841 gcctttctgg aatccagaat tccggacggc gttatggatg cggattattt tataaccttg
      901 cctaaagaag acgattccat tgatagcaag gttgcggata aggaaagaga tttacaggcc
      961 gtcaaacaat ccgcgcaatt gcagcgtggc tccgaattgg aacaaatgag ccttccggcc
     1021 gtcccggatg agtttttgcc gattttgacg aaaaccattg acggcgttga agccgatgcg
     1081 gaaagacaga taaccgccca tatcgaaact cacaatatgc aggcgcgcgg taaaacatgg
     1141 ctttcggaag gttgctctta tatccatgat gataattgcc ccttttgcgg ccaggacttg
     1201 accggcgttg atcttatcga agcctataaa gggttcttta gcgccgctta caatgccctt
     1261 aaagatgaaa tcaaggccct tagtgatgag atctcggata gttttggcga tttgggcttg
     1321 gctcaaattg accgggttca gcataagaac gccgccgccg tggaattttg gtcgcaatac
     1381 tgcgatataa ccgccccggc cttgcaggaa tcctacgacc ttatcgtcgt tttggcgcgc
     1441 ttgcggcaaa gcgccctcac tcatttggaa cgaaaagcgc aagcacccct tgagcaaatc
     1501 aatccggacg cttccttgct ttcagatttt gaaattctgc gccagtttca ggcggcaatc
     1561 gtgcaataca atcaagctgt agatacggca aacgccctaa tccgcgcaaa gaaagagcaa
     1621 gcgcaaggcc gcgaagtgcg gcaagtggaa acggaattgg caaagttgaa agccgcccaa
     1681 aggcgctgtg accaacaaat agacgcggaa tgcaccgcat accgcaagct tttaatggaa
     1741 aagaaagact tggaagaccg gaagaccgcg gccaaagaaa agctggatga atacacacaa
     1801 agcataatca agaaatacga aaaaacgatc aatgagtatc ttgattgctt taacgccggt
     1861 tttcaaatta ccgaaacgtc acacggttac aggggcggcg cgccaagcac aagctatcaa
     1921 atccttatca atcaaacgcc ggttgatctt ggcaattcgg atacgccaat tgaccagccg
     1981 agtttcaaaa acacccttag ttcgggtgat cgtagcgcgc ttgcgttgtc gttcttcttg
     2041 gcgcaattgg aacatgaccc ggataaagca aacaagattg tggtttttga cgaccccttt
     2101 aacagccagg acgatttcag aaaaggccat acggcacaac gcatcaaaaa atgcggaagg
     2161 gaatgcgcgc aagttattgt gttatcgcat gaccagcatt tcttaaagct tgtttgggac
     2221 gatttgccta cggatgaacg caagactttt cagcttttcc gcatatcgga gaaaaacacc
     2281 ggcattgccg aatgggatat tgaaaaggct gtacaagcgc gttaccgggc cgacattgat
     2341 acattgagca aatattacac aagcgccaat gacggcaacc aaagggatgt tgtccaaaaa
     2401 atacgccctg ttttggaggg atattgccgg aatctgtatc ccactaggtt tgcggataac
     2461 gatgcgcttg gcaccattgt tgggaaaatc aggggatacg acgacgaatt cccccttaag
     2521 gatgtttgtg atgagttgga agaattgaat gggtattcgc gccgttatca tcacggcgaa
     2581 aatcctaatg ccgccacgga agctattaat gacaatgagc ttaagggata tgtgaaaaga
     2641 accttgaaag ttgttggagc acttttatag aaaaatttcc atttttttgc tttttagtat
     2701 ccttcttatt gaataatttg aagggcaatg ttagcttaat gacgatgcgc aagtttttga
     2761 tgatattctc tgtttttgcc ttggtcgttt ctataacggc tgacttagcg catgcacata
     2821 ccacttccct agatacgcaa acccaaattt ctgctgatat ggatagcggg cctgataaca
     2881 gtgcttatcc gggctgctgt gatatggctt gtggtggctg cggtatgcac catcaccacc
     2941 atgcagccaa tggccttagt gacggctttt ccttacaggc cgcaacaaaa gacaggcagg
     3001 gttttggcgc agaatcaatc tacctttccg atttcgttta tggcctaaaa cgcccacccc
     3061 ggatttaatt tcaggggtga agtgtatctt ggcttgggcg taatacgtct aatctaatct
     3121 ccaaacacaa cactcctgta tgtttcaggc aaaagcccgg cttttgcctg catgtcgctt
     3181 ttttatcaaa cgaaaggaac gaatcaatga aaagcttact gacatttact tttatagccc
     3241 ttttaggggt ttcgggagtg gcgaaggccg accaaaacgc agaaacaatt gcggttgagg
     3301 atatgccgcg ctttgccagc atcgagcaac agcgctgccc taaatcccgc tatccatcag
     3361 ttaaaaaacg ccttgaatgc aaacatgaag tgcggcgtga aatctttgag aaaaggcagg
     3421 cgcgcgaaca gcaaaatcag agcgccgctc aatctgaaaa ccaaggagaa atacaatgat
     3481 acaacaccta aaaattctgg ctataacagc tttggttatg ccttttatgt tcggcatagc
     3541 gcaagcgcag gaactagcct cgaaaaagcc gggtattgaa aatttcatta actacatatg
     3601 gcaggcaagc ccttccgtac aggaagcaga ggcgagggtc gccgctgccc aggcaggaca
     3661 aaaagcagcc tcgcgctggc aacataatcc ggaagtagaa tatgaagtgg aagatgttga
     3721 gggcgaagaa aaaacccagg ttctcggtgt tagtcaaaca atagactgga gtggaaaatt
     3781 cctgtcctcc ggcaaaacgg ccaaatttga attaatggcg gcgcaggccg aacgtgatga
     3841 aattaggcaa aatatcgcca ttgatgtttt ggcagcctta gccgaataca atgcgactaa
     3901 ggatgttttt gggctggcaa cgcagcgttc aagtttaatg gagcgttttg cagggcttgc
     3961 agataaaagc tttaagacgg gcgatattga ccaaagtgaa tataatctgg cgcaattggc
     4021 tttgtcagag gccttgattg agcaggcgga ttcagaaaca gcattggccg aaagcaggca
     4081 ggcgctggac gcagcaatcg gctttgctcc cgataacttc tttgctttac cggagttgcc
     4141 tgaaacactc ccggagcctg tacttggcat tgataacgaa gatcaggttt taatgcagct
     4201 gccctctttg cgcgcaagga agcaccggga agaagcggcc aaggctgtta ttacccaagc
     4261 tcaaaaggat cgccttcccg atccgaccgt cggtcttgtc ggcgggaaag atgccggagc
     4321 cgatatgatt ggtttctcgg tttcgatgcc tttgtatgta ttcaatactt acggcgcgga
     4381 ggtagaacag gcgaagcaag aggccttggc cgaagcaaag gccggacaga acgcttacca
     4441 tgcagcaaga acacgcctgc aggcaagccg cagcgcttac cagctttcca gaaaagcatg
     4501 ggaaaactgg caagagaacg gcgctcaggc tttggcagaa caagtcgata ttctcgaccg
     4561 taaagtcaag gtcggagatt taagtgcaac ggattatttg gtgcaaattg agcaagcgct
     4621 tgatacggaa gtggcggcaa aggaactgca cagaaaagtc tggcaatcat ggtttgcgtg
     4681 gctggctgct tcgggcagcg ttgagcaatg gatacaaaac acaggagaaa atcaatgaga
     4741 ttattattag ttttaacaat attggctgct ttcagtttta ctttttattc ttatgccgcc
     4801 gaggatggtc acggccatga cgagggatcg catgttgaaa aagaacaaga tgaaccgggg
     4861 gccggacatg aagaaagcgg tgaacatgaa gatcacggcg aaaaagaaag tgaagaaggt
     4921 cacggcgatc acggcgaaga aggaggccac ggacacgaag aaaaaagtga gatagagatt
     4981 tcgccagaat ctctgaaagc cgccggaatt cttgtggaac ctttaacctt gcaaaatctg
     5041 gaaggtgaaa ttcaagcccc cggcgaggtt gttttgaatt cctatttaag cagtaaagtg
     5101 acaccgcgca tcgaagcgca aatagtatct cgtcacgcta agttaggaga taaggtcgaa
     5161 caaggcacac cgcttgtaac actttcttcc gttgaaatgg ctgcggccat tggtgaatct
     5221 ctagtggccc ataaagagtg gcggcgggtg caacagctcg gatcggcagc cgtttctgca
     5281 aaacgctata gcgaagccca aattgccgat gaacaagcgc gcgccaaagt tatggcttac
     5341 ggtatgacag agcaacaaat tgaggccctg cataaaaatg gggccaaagg aaatccgggc
     5401 gagtttcaac tccttgcccc acaagccggt acaattgtca gcgataattt tattgaaggc
     5461 gaattgattg agccgggccg cgttctattc gatattgtta atgagtccgc tttatgggtt
     5521 gaaagccgct tatctccaga aaatgcaaag cagattgaaa ccgaagcaaa cgcccgcatt
     5581 catgtgcccg gcaatggctg gctgcaagga aaggtcgttc aaaagcacca tttgctcgat
     5641 gaggaaaccc gcacgattgg aattcgtatt gaagttgata acgaggaaga ccgcctccat
     5701 cccggtatgt atgtcgatac agccatacaa acgggcgggg agcaaaaata cctagctgtt
     5761 cccacatctt ctgtccttcg ttcggcggac ggagattggg tggtctatgt cgaagaaaag
     5821 ccgggacagt ttaaaccaca agaagtgcaa acgctaagaa cggttgaaga ctataccgtc
     5881 attgacggcc tgccggaagg cacaagggtt gttacagagg gtgcattttt cgtgcaatcg
     5941 gaactcgcca aaagcggctt tgaagtacac aaccactaag ccgggagaaa aaatatgctt
     6001 aatacaatta ttgacggtgc ggtacgtaac cgcctaatgg tcattctaat gctggcgtcc
     6061 attctggtcg gggcaattat gattttgcca aacctaaatc tggatgcgtt tcccgatgtt
     6121 accaacgtac aagtcacggt gaatacggag gccgaagggc ttgcagccga agaagtcgaa
     6181 cagcttatca cttacccggt tgaggcggtt atgtactccc tcccggacgt agaggaagtg
     6241 cgctcgattt caaaaaccgg cctttccatt gttacagtcg tttttacaga gggaacggat
     6301 atttactttg cccgccagct cgtttttgaa cgattgcagg atgcacggga gcagattcct
     6361 gagggcgtgg gaacacctga aatggggcca aacacctctg gcttgggaca agtgttccaa
     6421 tatatgctgc gcacgaatga tcccgataca ttcgatatta tggcgcttcg cagtttgaat
     6481 gactgggtgg ttaaattatt gcttatgccg attgatggag taaccgaagt tttgtcattt
     6541 ggaggtgaag taaagcagta ccaggttaat cttgaaccgg caaaattgct ttcctaccaa
     6601 cttcatgccg aagatgtagt ggaggccatt gaggaaaata accgcaatgc cggtggctgg
     6661 tatatgaaca aaggctcaga gcagcttgtt attcgcggcg tgggttgggt tcggggcggc
     6721 gaagacggtc tgcgcgatat tgccaatgtc ccggtcaagg atgaagacgg tgttgtagtc
     6781 aaagttagtg acgttgcaga agtttcttac ggctctgaaa ttcgccaagg agccgttagc
     6841 attacgatgc gcgatgaaag cggtaaaccc gaacagatgg gagaagtcgt taccggcgta
     6901 gttatgaagc gcatgggggc gaatactaaa aacacaattg accagatata tgagcgcttg
     6961 ccaattattc aaaacgcctt gcctgaaggg gttattattg agccgtttta cgatcaggca
     7021 gaccttattg gtaaagctgt tcacacggtt agcaaggctt tgattgaggc ttttgtactg
     7081 attgtcattg ttctgttttt atttttgatg aacatacgag ctgcaatttt ggttctgctt
     7141 tcggtcccta tttcaattgc tatggctttg atggcaatgg cctattgggg aatttcggca
     7201 aacctcatgt cccttggtgg tcttgcaatt gccattggta tgatggtgga cggctcggtt
     7261 gtgatgatgg aaaatatatt caaacatctt tctcatcacg atgaaagcga caatccacaa
     7321 ggtacaagct tgcgtatttt agaagcagcc cgcgaggtgg gacggccagt cttttttgcc
     7381 gttttgatta ttattgttgt tttcacaccg ctatttacgc ttgagggtgt ggagggcaag
     7441 ctattccagc ctatggccgt atccattgtg ctggctatgc tggcttctct tatggtggcc
     7501 ttggtggtta ttcctgcact ggcaacctat cttttccggc gcagtgtcga tcacaaatca
     7561 aacaaactga ttgatgctat agacgcagga tacaagaaaa gcctgtcggc agcccttaaa
     7621 gctaaaaagg cgattgtgat aaccgctgtt gcgttgttta taggctctat ggctcttgtc
     7681 ccgtttttgg gaaccgagtt tgtgccagag ctggaagaag gcacggttaa tatccgtgtt
     7741 acgctagccc cttcctcgtc attggaaacc tctttggaag tcgctcaaac tttggaacgc
     7801 aagctgatgg agtttcccga ggtgctttac gcctcaagcc gtgttgggcg cgcggaactt
     7861 ggcggtgatc cagagccggt atctaacgtg gaaatctacg ttgggctaaa accggtcaag
     7921 gagtggactt cggcttcaaa ccgctttgaa ttgcaaaggt tgatggaaga aaaaatgtcc
     7981 gttcatccgg gcttgttatt taccttctca cagcctattg caacccgcgt ggatgagttg
     8041 ctttccggtg tgaaagcaca acttgctatc aaactattcg gccccgacct gaatattttg
     8101 gcagaaaaag gcaaggccat tgaaacgtta gtgcgtaaaa ttgaaggtgc acaatcggtg
     8161 gaaatggaac agattgctgg tgaagcgcag cttgttatca agcctgatcg tgaccagctt
     8221 gctcgttacg gcattccagt cggacaggtt atgtcccttg tttccgatgc tattggcggt
     8281 aatgaggccg gacaggttat tcaaggcaac gagcgctatg acatttatgt ccggctggcc
     8341 aaaaaatacc ggaacgatcc ccatgctatt gccgacctaa ttatgcaggc accaagcggt
     8401 gcgtgggttc gcattggtga tgtggccgat gtttcgattg aggcaggccc tccgcaaatc
     8461 aggcgggatg atgtgcagcg ccgcgttgta attcagtcta acctgaccgg acgcgatatg
     8521 ggcggttttg tggaagaaat caataagcgc attaaggaag aagtaaaatt gccgcccggt
     8581 tattccgttg ttttcggcgg gcaatttgaa aaccaaaaac gggcgcaggc acggcttatg
     8641 attgttgtgc ctctctcgct tggtcttatt tttcttttgc tgttttttgc tttcggctcg
     8701 gtgcgccagg cagcgctgat tatgctgaat gtcccgttgg ccatgatcgg cggtattgct
     8761 gccctgtatt tttcagggca atacctgtcc gttcccggat caatcggctt tatcgctttg
     8821 tttggcgtag ctgttttgaa tggcgtggtt atggtcaatg caatcaactt gctcattgaa
     8881 ggtggcacag acgcggatac agccgttttt agcggcgcat tatcccgttt gcgccctgtt
     8941 cttatgaccg cctctattgc ggctttaggt cttattccga tgcttttggc aacgggtata
     9001 ggttcagaag tccagaagcc actggcaact gtggtcgtcg gcggattgtt ttcttcaaca
     9061 cttttaacac tgttcgtctt gccggttcta tacggcgtat tttcacggcg ctttgtagaa
     9121 gagcacaaga gaaaagtttc gtaacgaaag gagcaaaaaa tgaaaaaaac catacctata
     9181 ttaattgcct gccttgcttt ggccggatgc gagtctttca gggcaccaac cgttgagcaa
     9241 aggctggcag ggaaaaccgg cgcagaacgc cagcaggagc tttactatat ctgccttgag
     9301 cgctttggcg accctatcta tggcggcggg cattaccccg gccatgaagc ccatagagag
     9361 gcgctatgcc accatatgga tgatctcaat aaagaaacgg aggaataacc atgcctaaga
     9421 aaaccttact tttacttcct tttgttgccg tgctgtccgc ttgcagctgg tcgcattacg
     9481 gaatgctgga aaagaaactg gacggcaaaa cagaagatga aaagcgcctg attttggccg
     9541 aagaatgtaa aaaggaaatt gagcgcgggc ttgtcaaaga caaccctaaa aacatagagc
     9601 aacataaaag aatgcaatat gtttgcgaag aaatgaccgg aaggccagtg gaggttagat
     9661 attaaaaatg ggacatcatc accatcacgg aaacccgtct gaaattggtg agcgccgcct
     9721 ttggtgggcg gtactagcca atatgttgct aacagtcgcg caagtcatcg gcgggattat
     9781 ttccggctca ctttcgctgg ttgccgatgc attgcataat tttagtgatg cagcctcgct
     9841 tctcattgcg ctggtggcaa tacgcatagg ccgtaagccg cccgatcaat ttaaaacgtt
     9901 tggttacaaa cgggcggaaa ccgtggcggc gcttatcaat ttgaccacgc ttatcgttat
     9961 cggcctctat ctttgttatg aggcggtcac acgctttata acgccgcagg aaattgcagg
    10021 ctggacagtt attgccgttg ccggaattgc gctggtcgtt gatattttca cggcggtgct
    10081 aacgtataat caatccaaaa caagcatgaa tatcaaagca gtttttctgc ataatttaac
    10141 ggatgctttg gcctcggtcg gggtcattat tacaggaacg cttattttgc tgttcggatg
    10201 ggtttggaca gatgccgcca tgacgctgat tattgccggt tacgttttgt ggcagggctt
    10261 tacagagatt cctaaaacca ttcatttgct catggaaggt acgccggagg gcatggacat
    10321 taaggaagtg gttgcagcaa tggaaagcga aaaaggcgtg gtcaacgttc atcatgtcca
    10381 tgtttggcaa ctggacgaac accgcaatgc tttagaggct catgtggttt taagtgaaag
    10441 tgcggaaatg gatgatttaa aaacccgcct caagaaaata ttgcacgaca aattcgatat
    10501 tgagcattca acattggagt ttgaaaatac cgtttgtaat taagcaattc gccgtcctcg
    10561 ggctttacag cctgagggcg gtattcaggt ggaataggct tgtaattcgt tcataaagtc
    10621 catttcatct tttgcgattt caccatcggc ctgcacaagg cgggcggcat atcgagccaa
    10681 tttccccaaa gcttcggggt ttttctcttc caaatagtca agggagtcaa agaaactatc
    10741 ggtatccgga taaagttttc ttatgcgcgc taacaaatat tcgtcatgga attcacaatc
    10801 aaagcactca tccatcaaat aatgaataat aatgttttct tcttcatggt gaaaatgacc
    10861 gtcgcaacgt gccaggtaca ccagaacatt catagcgtgg cggcactttg taagggcttc
    10921 gcgggtggct gctttgggat caaccggcaa ggtcttctcg accacgccga agcattcaag
    10981 gatttccgtt tgagtatccc aaacctcgcc agtcgccaaa tcgaccgcct ctacaattct
    11041 atccgcgcga aaacaccgaa cggcttttcg ttcaaaacaa taggcccgga caagcaaatc
    11101 tcctttggca gaccggctta cgcgcttaac gctaatacgg cgctcgcttt cttccccttt
    11161 ggagttttta tatgtaataa agacggccgt gcttatgcta tccgcaagtg cctccgcctg
    11221 ctcctccgcc tcaatttctg tctgaggttc gtcttccacg agccgtatgt gccggttttc
    11281 cgggacttcc ggctccggca ttggcatttt ccccaaaacg tttttcaaca aaactgtaaa
    11341 catatcttat gtttttagca tttcctcttg aatcttcaat gcttttagtt gcaatacggg
    11401 caaattcagc tctctacatt ttcctaaaaa tctgcttaga taagagtgct ttgtcgaaaa
    11461 aagggcaagg ccgggctgta gtaggcccga acattagcgt ctggcaaaac gtaatttgtc
    11521 gcttctcggt cttatggccg gggggcggca gcgtatgtcc aaggctccgg cctaaaggct
    11581 gtcgcggctg tactaatgtt cggtctacta acctccggcc accatgcccg ataaaacggc
    11641 atggtggttt ttatttggag tatcaacgct atgcaaaaag caatgattag tttaaacatt
    11701 aataccgtga cagggcttga taacccgaac aagttacacc gcgctaagta ttcacaaaat
    11761 tgcgcgctct cttatgtcga gagggcgcgg gaatacaata ccgttttgcg gaaataccgc
    11821 gcgcaccgct tgttggtgtt atcaacctct cggctccaga gagacgctct gttcatggca
    11881 ttagcacatg aaaggagctg ttatgtcatt aagcagtttt aaacaaactc tggaaaaccg
    11941 tttatacggt gatatttggg gagccgcaaa gccggaggaa gccgcagggg ttttggtttc
    12001 cgtttacgga gggcgcgcca tcaatgaagc ttttgaaagg gaaacggagg catggaaaca
    12061 atcagacttt agccgggctg ttttttgggc ttgtgtatta gaaaagctat ctcccgactt
    12121 tttaccatgc cagcattagg gaggatcata tggctaaatc aaaaaacaca gggccgcgcg
    12181 ccacaccgat tgatattcat gtgggcaagc gcataagaat acgccgctgc cttttaggaa
    12241 tttcacagca acggcttggc agttcaatca ataaaacttt tcagcaagta caaaaatatg
    12301 aggaaggcag taaccgtgta gcggcgggcg ttttatatga cgttagtaaa gctttggatg
    12361 tgccttttga ttattttgtt tacggctatg aagaaagcca aatccaaaaa accgcgggcc
    12421 tttcagacaa tgaacaagag ccctttctcc atgtattaga ggattcacga aaaaaattaa
    12481 atgcagcctt aacagtcgaa aacctgtcaa caaatggcgc gttgatttgt ttcatacgaa
    12541 aatacacgga ggccgttctt ctcaaccatc acaacgtaca aaaagtaaat aaatttctgg
    12601 acgagttaat cgaaaaggaa aaagagagag gcggggaata atggggagat tgtttttgac
    12661 gctgttactt ttggccggat gcacaaacta ttcagcattt gaaaggaagg ttatagagcg
    12721 cgcctattca gaaccggcga cagcgcccga atattttatg caccaaatcg aaagcggtgc
    12781 gaccgtggaa gagcaagccg tttatatgta tggcatcgga ctcgctcacg aaaagcttgg
    12841 caataaagcc gaggctctcg gtgattatct gagcgctgag gcattgggat ataacaaagc
    12901 cgcagaagcc ttagaacgtt taaaaactaa gtaagaggta tgccggtgcg ggcagaccaa
    12961 aattccatga ttttgcgaag gtggccgggg ctaataaggc agaaggcatc aaaaataggg
    13021 ccaattctta cacctgtttc cgaccgtgtg ccaccgatac gcccaagcat tagccttatc
    13081 ctatcaaaag tccagtaacc atcgtttggg ttaagaatga ccggattgtc cggggagttg
    13141 atataaaatt cgtgctgtgt aagggaaact tgacgaataa tgattatata ggatgcgccg
    13201 agcgctcctg ttacaaggga gggaaattct ccgtccgggt ggtcttcagc tctcccgtaa
    13261 gttattcggc cacctgatgc accgctggtc gataaacgcc aatgggtgtt aaaggcatta
    13321 aatttagaaa acccaaaaat ttgattgttt tcaaatgggc caggcatatc aatcaccata
    13381 ccgattgtcc tttcaacctg aaacggtgtg gggtctgcat cgtacatcat ccagcgcgcc
    13441 acatcttcaa agccataccc gcctaatccg ttaaaaaaag cagggttaaa atgcatggca
    13501 gattctggtg caggctgttc aagaatatat gttcctttaa tgggatgaat ttcttccaaa
    13561 taattttccg aatgaaaaac tttttttgaa agtgccgggt cgctgtagtg ataaagattt
    13621 ccaatggaaa gaatcgggtc acgttctcgc cgcccgtaag caaacatatt gccggtattg
    13681 actaacattt taattatcct tcgtggtggt ataaccaaga gctttattaa tattttcgag
    13741 ctttgatcgg caatcttggt gcgcgtacca gagttttgct ccccatgcgg caatatcctt
    13801 ttgtgtgact tcatcgccgg gataggccgg agaggtttga cattgccgca ggcttggcgg
    13861 gatctccggt ttaatcactt ttacttgtgt ctgcgggttt cccgtacagg cgcttaattg
    13921 tatcgcacag tacaggagcg caagggccgt ctttttcatc gtctgctcct tctatagtac
    13981 caagcaagcg ttcaagctcc ttggcgcgtg tgacgctttg gttattttcg ttttccaaag
    14041 cttcaatttt tgctttggtg tccgtttgca attcggcaag gctcttttcg tatgaggcgg
    14101 cgctggcttg tgaagccagc aattttcttt ctaggttcgt aatttcgtga tcctgccaag
    14161 ctatataacc tacgagaata agggccgtga aaacggcgct taaccatttc cagtttttca
    14221 aaataaaact aatcattttt ccttattccc gtttttgtgg gccgcataga gataagctcc
    14281 aatagggcct gaggctaaaa tgccgatata ggttaggatg gattgaagca aaccaatgtc
    14341 ggccccggct aaaatttgat aggccaccag caagtttggg agtatcaaaa aggcatagac
    14401 agaaacccaa aaggcaagaa aacgcctaac tcttatggga tcggtattgt caggtttaat
    14461 cattaattcc tcctacataa agatcaaagg cgggccgagc cttttgtata aaaccctcga
    14521 cagtgccggc acctgccggc gtgttgtaat atttcttcca ataagcggcc atgccgggcc
    14581 agtcgttcat ttccggcaac ggctctttaa agcgaagata aaaaatgcgg cacatggccg
    14641 ccgcataaaa cagattgcca tgcagttggt catgcaaatc catatcccgc attatgaggc
    14701 cctttagctc attcagaagc gcgggcctgc ggcttaaata tctttcccaa atatcgtcat
    14761 gcgtaaccgg ctccatttgg aagaatccgc gcgcagggcc gcgcccgact tgatgaatga
    14821 aaaacgccct gctttctgcc agggcggtgc ctataaccaa ctgttcggcg tttggtgtcc
    14881 aagcgttaat gcgttccaaa gcgggccgca ccacataatc acgaagctgt cgaatgtcca
    14941 ttattgcccc ccgttttcat ccactttttt gcggaggtgg tgcatatctt cccgcatatc
    15001 tttaatttgc gctttgatcg tggcgaggtc ttctttcatt tctcctacca cccggcgacc
    15061 cgttacatca ttattgactt gctgttcaag tatttgaatt ttctgctcgc tggccgtaac
    15121 atgggacgta acccaaacca caagctttac aagcgccata atcacggcca cgctgtgggc
    15181 aataatgccg aacagcagcc cccattctgc tggtgtcata ctttttcctt ttctgtttgg
    15241 ttaatatgtt gaaatgacaa tccactggcc gccgtccgaa taaatcgtta tggcggcaaa
    15301 atggcttgtt aatgaccaga tttgagcgtc cggcccaccg gagccgcccg ccatttggac
    15361 tttcacatga ttaccggaag gatcaatttt cttgatcgta cacattcggc ctgccgcttg
    15421 cgcgggggta ggaatttcaa aaatgatttg ccctgaaaaa gctgaaacca aataaacccg
    15481 gttaagcata tctgcctgaa acgtaccgga aggtgcggcg gcattatcaa tatagcggct
    15541 aggccggggc atacggttgt cttggcaaat ccaccaagtc gcaccattag aaacgagcgt
    15601 aaccgtgtcg tattgctgta agagcgtaag cgtccggtta tccggcccgt tgccgctatc
    15661 ttctttcacg taaattgggt tatcccttaa atcgcttttt ttgagcgtaa cccaccgccc
    15721 ggttgcgttt ccggcattcg gcaaaatcag gtcataagat ttgttgaagg agcttaaaag
    15781 ataaaaggta tgctccaaat caattgaaat gtcgccgaca tgatcctcag gcggatcaat
    15841 ataagttgtc ccgcgcgtca tgccgtctat atgcacatcg gttatatatg catccttgat
    15901 aaaattcttt ttgggatagc ccgcgtcaaa ggcttgatat tcgccctgca aggtggggtc
    15961 ccaaatagct gcgccgccgg tcgctgagaa aaggcggata attgtattat caatagaacc
    16021 gttatctatc cgaatacccg gcgcaagccc taagcattct gcgtaaaaat tggtaataac
    16081 attatgccgc gcaagaaaac cgagccggaa acacgcctct gcatctgtgt ggacgttagc
    16141 ctcacaatca ataaaggaat tattatattg cccggttgag ataaagaacc cggaaccgga
    16201 aagaggcgag gaaagtgaat aaacgcgcac atcgtaaaac ttatttgcat ttggtgaatc
    16261 cccgaacgtt tccgggtctt cccaatcatc cggcgtggtt gtaagcaaaa ccccgttaat
    16321 gccggggcgg gcgacaagaa cacggcttat attattccaa tagcaaggct tttgagtatc
    16381 attccatcca tccaaggtta cgccgatttg tgcgtcccaa atggttacat tctcaataac
    16441 atttttaacg caagggccga cttttcccgc aaggaaaagt gctgaggccc cgccaatgat
    16501 ttttaaatct tggacaagac aataaccctc aacaatttcg atagcgttaa aatccgcaga
    16561 ataaggcgga agctcgtttt cgtttaaggc ttcctcttgc gcctgtataa tactcatatc
    16621 gccatagccc aatatactca tgccgtaatt caggacgata ggctcggcaa tacggtatgt
    16681 tccgggcgat aaaatagcca gtttgttttc ctgtaagcat tggttaatgg cgggcgcgtc
    16741 atcatcaata ccgtttcttt ttgcgccgta atcctctggg cgcggaaact ctcgtgcggc
    16801 aagatact
//
LOCUS       DAOSWR010000033        16608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2189_length_16608_cov_7.343865, whole genome shotgun sequence.
ACCESSION   DAOSWR010000033 DAOSWR010000000
VERSION     DAOSWR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_16608_cov_7.343865"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..1133)
                     /locus_tag="PK513_03980"
     CDS             complement(414..1133)
                     /locus_tag="PK513_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO81642.1"
                     /translation="MLDYDSIVPLYEQIMQEIKKDIEKKNYNTKHRLPTEEELAEQYG
                     VSRITIRRAMSELVEQGLVKRKQGKGTFISPPKMHKDLKLSGFSFTELCEANGKIAGA
                     KLLEAGLIPLDDPQVSEWLHIPKGDRALRIQRLRYASGVPCVIEDNYFPKEYVYLLEI
                     DLEHDSLYRYLREEKDIEIVTGEMILRIVKADAKTAKLLEVPHNAPLLRTFGRIFRGD
                     GEVLHVCKQVGYGEDFDFIVR"
     gene            complement(1389..2390)
                     /locus_tag="PK513_03985"
     CDS             complement(1389..2390)
                     /locus_tag="PK513_03985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO81643.1"
                     /translation="MIAGMKEVIETINCVKKELDARGGLKTVIFAACGGSFSASYPAR
                     YLLNAESTMFHTAGYNSSEFVSATPKCVDRNALVICISTKATPETVEALNVAKKAGAI
                     TIGLTGYGDSLTAQSADYSIVYYHSDEWYEKPELMHQNSQGTALKIAFELLHQMQNYP
                     YYDKAMDAFEKLPAIYHAAYKKVKQRAVKFAMTYKDDTVWNVMASGAAWESAYSDAFC
                     FFQEMQAVHCVPVHTGEYFHGAFETTDKDLAILLLKSVGRTRPLDDRVERFLDQYAGH
                     HYVIDASELGLDELDETVAEYFNGLLIHPVTKQFIAAMADIRMHPMSYRRYMWKFEY"
     gene            complement(2416..4161)
                     /locus_tag="PK513_03990"
     CDS             complement(2416..4161)
                     /locus_tag="PK513_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOO81644.1"
                     /translation="MTQKRRVDTVTVEVVRNLLMSIAEETYGIIIRSAYSTNMKERHD
                     VCTAVIDPDGNSVAQMESLAALLGSMLSIVPNIYKKFGRENIKPGDMFIANDPYHGGG
                     NHLPDIVIAAPAFSGDKLVGWIANIAHHSDIGGKVPGSTSGDADSMFQEGIRIPVIRI
                     CENGALIQSVMDLLLDNTRVAQEREGDLTAQMSANLIGIERICDAYARYGDVLIDCMK
                     EIIDYSERRVRAVVAKLPNGDYEYTDYVDGCGDKYPDPLPIKAKITIDDDDLTVDFTG
                     SAAQIEAPINVPWPCTKADVFFAIKAMLGPDIPANEGINRAIRIIAPKGSILNPNEPA
                     PVGAQIDSSQRVPDAIFGALAPVLPEAVVTAGNGACTTTILAGKGEIGSDRVFIFHEV
                     IAGGGGASIHYDGLSGVQVNMTNTSNMPIEATELEFNKILARKYELKEDSGGAGKNRG
                     GLGIEREIEILQDNILYTGLGDRHKFHPWGLEGGLDGEVGSFWRISKDGTKTKMGHKT
                     SSLPLKKGDVIRVVTPGAGGYGNPRMRAAEKVLNDVIEKKVSVEKARELYGVVICRTP
                     EGDWQIDKQATAKLR"
     assembly_gap    4228..4237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4238..6230)
                     /locus_tag="PK513_03995"
     CDS             complement(<4238..6230)
                     /locus_tag="PK513_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOO81645.1"
                     /translation="MGYMIGVDVGGTFTDFSVFNQDTGELYNYKDSSTPEDPSRAIVK
                     GIDDILSIKHALSAQVAYLAHGTTVGTNALIERKGAVLALVTTKGFKDLMEIGNQRRP
                     SLYKLQEQKPCPLIPSGLNYGVSERILYDGSVETPLDENEVREVVRELKRKKVQSIAV
                     CTLFSFINPSHELRIEEIIKEEYPEAYVTISSKLAPEFREYSRMSTTVLNSYLGPVME
                     KYVYNFQKSIKDLGITAEPYVTQSNGSIISIKETIECPIKTAVSGPSAGVIAAAYIGR
                     QCEADKIITFDMGGTSADISLIENYTPQVSNERLVEGYPARIPMISIITIGAGGGSIA
                     WIDEGGALKVGPESAGATPGPACYGRGGTRPVVTDANILLGKLNQSRILGGRMQVYLE
                     KALDALNENICSKSSLSSDEAAKGIISVVNSNMIRAIRSVSVEKGYDVREFSLMAFGG
                     AGPLHACEVAGELGISEVIIPPHPGTLCSLGLLMADTKFDLSRTRILAGTVENLDEVN
                     RQFESMVCQGTELLDREGVPAERRSFEFSIDMRYHRQNFEICIPISAGDMDVNALTKA
                     IEDFHAEHKRSYGYCNKAAAVQFVSYRVSAVGTIDKPNFTPEPLRPNNPLPNAIEHRS
                     VLFQNHSLRIDTPVYNRADFAAGQEITGPCIMEQMDTTLV"
     gene            complement(6311..7147)
                     /locus_tag="PK513_04000"
     CDS             complement(6311..7147)
                     /locus_tag="PK513_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO81646.1"
                     /translation="MTGLSADRIAIGSYPYYRYSMDYFLDTASQLGFSKVELWTAAPQ
                     IDLMDLSPAFCRDLRSKLKARNLTVCAITPEQCNYPFNLAAQEDSLRQRSVDYYCTAV
                     DLAAELQCPWVLITSGSGYYDLPKDPALRRSLDSIEKIARHAVAHDVKLVYETLTPMS
                     SNLVNSPAQVREMIDQLPANSVFGLLDIGQMTVMGQTVAEYGAALGDKLAHVHLHDTH
                     PSCHMALGDGDLDLERIIGEILALGYGGLFSLECNDARYRLDPRAADAKNAAWLRDHG
                     IT"
     gene            complement(7144..8049)
                     /locus_tag="PK513_04005"
     CDS             complement(7144..8049)
                     /locus_tag="PK513_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO81647.1"
                     /translation="MVKVLGLGDNVCDIYLNQGVMYPGGQALNVAVYAKQLDVQSDFL
                     GVNGTDEVAEHVLRTLDELGVGHSRCRRTRGENGFACVTLIDGDRVFLGSNKGGVLQK
                     TPIKLGQDDLTYIDCFDVVHTTNNGFIDEQLPLLAPLSPLISYDFSYRWNEQDRLERV
                     CSYIDFSFLSCSELSEDETFELCEHVYKKGCGVVVATRGSKGAFVWDGARVYRQPPYA
                     VQAIDTMGAGDSFAACLLVRLAQALKSDGKGRWTDANYRAKILPPALADAAAFASKTC
                     LVHGSFGYGTPVPASLKTRIKEVVS"
     gene            complement(8064..9506)
                     /locus_tag="PK513_04010"
     CDS             complement(8064..9506)
                     /locus_tag="PK513_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO81648.1"
                     /translation="MNKEQFVIETIESNRDRYTEAAGHIWDYAETRFDEKRSADELKA
                     ILRHEQFVLTDGVAGMQTAFVAEAGQGRPLIGFLGEYDALPGLSQHADSVTRSPITPG
                     APGHGCGHHILGTGAMAAAITVKRYLEENNLPGTIRFYGCPAEEGGGGKIYMARAGLF
                     DDVDCAITWHPADDNNIWSMNFMAIQSLRIHFKGISAHAPSQVHIGRSALSAAELMNV
                     GANYLRGQVQRDVCINYAFIDAGGVAPNVIPNYSEVVYNIRANNHKRAMETARRIDEI
                     AHGAAMMTRCEADVEYTGGLSELIPNRTLERIAYEQFVKIGPTPHTMEDENFCREIHK
                     SFPENAEESTFSTLEYLYGPEAAPLIPQIRGKVINDVLYPYKAIPHAKYGSTDVCDVS
                     WFTPTIQLTTACYAKDTPGHSWQEVAQGKRPLCYNGMLTGAKVMALTGIAVATDGEAM
                     RAVRREFTEAMKDKTYECPIPEDLKPEASF"
     gene            complement(9520..10503)
                     /locus_tag="PK513_04015"
     CDS             complement(9520..10503)
                     /locus_tag="PK513_04015"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOO81649.1"
                     /translation="MNQNLLEVKNLKKYFITKGGLFSGPTRYVRAVDGVSFSLKEGDI
                     FGIVGESGCGKSTLGRSILRLIDATDGEILFEGRDILKLSKEEMRLLRQKMQIIFQDP
                     GASLNPRMTVGEIIQEPLDIFSGKNEKEKEMKVLELMDMVGINHSYISRFPHEFSGGQ
                     RQRIGIARALAVMPKLIICDEPVSALDVSVRAQVLNLINELKQEFNLTYIFISHDLSV
                     VNHICNQIAVMYLGRIVEIAEKKELFENPTHPYTQALLSAIPMPDPSLQRERIILQGD
                     VPNPANPPSGCHFHQRCPYATERCIREVPQFTEVANDHFVMCHLVDQPLNS"
     gene            complement(10478..11485)
                     /locus_tag="PK513_04020"
     CDS             complement(10478..11485)
                     /locus_tag="PK513_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO81650.1"
                     /translation="MKDDVLLEVSGLKTYFFDRKGQVPAVDGVDFTLHKNETLGIVGE
                     SGCGKSVTSMSILHLLPPKGKIVEGNIRFKGRDITHLSDEDMTRVRGKEIAMIFQEPM
                     TSLNPVYTVGYQIREMILRHEKMSKKKAREKSIEMLRLVNIPAPEKRVDEYPHELSGG
                     MRQRVMIAMALACDPQLLIADEPTTALDVTIQAQILDLIRNLKKKMDTSVIMITHDLG
                     VVAEMADYVLVMYAGMVMEYSEVHKLFKKPLHPYTQGLMRALPKMGEENKKLYTIEGQ
                     VPSLYDLPTGCRFWPRCPYATERCKAEMPALYEVNGCKVRCFKYEKKETPGNESEPIR
                     G"
     gene            complement(11732..13225)
                     /locus_tag="PK513_04025"
     CDS             complement(11732..13225)
                     /locus_tag="PK513_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO81651.1"
                     /translation="MAIISSWNTLNIYNTSGNYGHCVADQMFERMVTCNHDGEYLPRL
                     ANSWEMSADNTSITFYMNENCTWHDGEPLTADDAVFTLQAVTNEAVDNYYRSEFSILA
                     GTDENGVCADPAALGAEAIDEYTLQLTFKSAKDQSTILSSLCSFFYILPQHILDTGNY
                     ADINTSDFWSAPIGSGPFKYVKDTAGEELELEANTDYYLGCPDFKTLVVKVVPASSLT
                     AGLLNGDIDVVGAGSIPLSDWETVKSADNLVAASVPGYSYQYMEFNLSEGETYFQDQA
                     VRVAFDKAIDKQLIVDQLMAGEGQVAVGPMPEYHPYFNSDLVGNSYDPTAAKSELEAA
                     GFDFTKEYRLIVPQGNQVREQSALIIQQNLADIGITVTIETYDFPTLLEMMRNGDFDL
                     GLLGGGSNIDPAESAVIVKPGSAQNYSLLTDSKWYDLAEAGSSLVGFSERKAAFDAYQ
                     AALVEDQPYIWLYHQNNLWAHSTRLVSIPQDDFIWYNYEVWTWELAD"
     gene            13215..13472
                     /locus_tag="PK513_04030"
     CDS             13215..13472
                     /locus_tag="PK513_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81652.1"
                     /translation="MIAMVTTSSFGPVASTVLDVVVSVEDVVSEEAVSEEVGLVLQPT
                     SASGSTKAIARRKHTFLFMFYTPFLFYLSHSFGDECNKLDA"
     gene            complement(13469..14329)
                     /locus_tag="PK513_04035"
     CDS             complement(13469..14329)
                     /locus_tag="PK513_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81653.1"
                     /translation="MSQKKAGFRNFIKRYSRNRLAVFGFFTFILIALVVLILPSFLPF
                     TESQIDATAFNQAPGGNHLLGTDDVGRDVLARLLYGGRVSLFVGIMSTAISVIIGIPL
                     GLLAAFYRGALETMVMRASDIFMTIPSTILILFLVSMFGPSIVTVTVVIGVLGWTKFA
                     RQIYGTVLSVREKDYVEGGIAIGEKDSRVITKYILPNAIAPIFISITFRIASAIILES
                     SLSFLGMGVQPPAASWGNMLYNAQSITILKSRAWMWLPPGLMLMTTILSINFIGNGLR
                     DALDPKTSIR"
     gene            complement(14362..15315)
                     /locus_tag="PK513_04040"
     CDS             complement(14362..15315)
                     /locus_tag="PK513_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81654.1"
                     /translation="MKRYVLKRILIAIPTFIGITILVFGLCYTASGSPLELLLNNQNI
                     SEAEVLRQAEKLGLNQPVYMQYLLWMKNFLSGNLGISYRTSGEVLPMIMNALWPTVLL
                     TLSAVLVACIISFPLGIQSACHQNGAWDNVSSVFSFLSTSTPSFFLALVFLYLFSVQL
                     GILPIGGMYDTGKSTSILSLLRHLIMPATVLGSTMVGSLIQYTRSSMLEVMREDYVRT
                     AYSKGLRNRVVIFKHVLRNSLIPVATYLGMEIPMLIGGAVVTEQVFSWPGLGNLMLKS
                     ISARDYPVIMGVTIVTAIAVLVFNLLTDLFYGVLDPRIRYN"
     gene            complement(16095..16295)
                     /locus_tag="PK513_04045"
     CDS             complement(16095..16295)
                     /locus_tag="PK513_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HOO81655.1"
                     /translation="MCCLTVCPKLCCIPNSTGHIKSTLTGNHCVFPLIDGEIICGGAQ
                     DIYLVEFDGPQLRRIYVETIGE"
     gene            complement(16339..>16608)
                     /locus_tag="PK513_04050"
     CDS             complement(16339..>16608)
                     /locus_tag="PK513_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO81656.1"
                     /translation="DEFKKNAVKLSYASPKPVRAVADDLGIAVNLLYNWRKKYTASGE
                     KTPMATMEEELKALRLENAELKMERDMLKKQRPTLRSSRSEILDT"
BASE COUNT         4120 a         4355 c         3743 g         4380 t
ORIGIN      
        1 gcacccaccc tcgtgcaatc gcgcgattgt atcacttcgt gagggtttag ccaaaaacag
       61 aaggtcaccg tttggtggcc ttttgttttt gctttcttgc aggtcaagcc aacgaaaccg
      121 ccttaacaag ccacatcgcg gctgttatct gttgcggagc cgagcgcgcc ctgtgggcga
      181 tgcagcgagg cggagcaagc ccagcgcggg cgttttagga agccgcttta tgcggcaacg
      241 cgaaacgccc ctgcgcgcag gccgcgggtc ggcgggtatc gagtccaaca gggggagcca
      301 aacaaaataa atccgaaccc gcttccaatt ggagatgggt tcggatttgt tgtttatatt
      361 gaatctttct agcaaaactt ttcacaacaa tcgatatgtg caaaagaatc acactagcga
      421 actataaaat caaaatcttc tccgtagcca acttgtttac acacatgaag aacctctccg
      481 tcaccacgga agatgcgccc aaaggttctc aatagtggag cattatgagg tacctcaagc
      541 aatttggctg ttttagcgtc ggctttgaca attcttaaaa tcatctcgcc agtaacaatt
      601 tctatgtcct tctcttctct gagatagcga taaagtgaat catgctccaa atcaatttct
      661 agtaaataga catattcttt tggaaaatag ttatcctcaa tcacgcaggg cacgccgctg
      721 gcgtaccgta atcgttgtat ccgcagtgct ctgtcacctt tgggaatgtg cagccattct
      781 gacacctgcg gatcatctaa aggaatcagt ccggcctcca gcagttttgc tccggcaatt
      841 ttaccattgg cttcacataa ttccgtgaag ctgaaccccg atagttttaa atccttgtgc
      901 atctttggag gactaataaa cgttcctttt ccttgctttc tttttacaag gccctgttcc
      961 accagttcag acatcgcacg gcgaattgta atacggctaa caccgtattg ttccgccaac
     1021 tcctcttctg ttggtaaacg atgctttgta ttgtaatttt tcttttcaat atcctttttt
     1081 atctcctgca tgatctgctc atacagtgga acaatgctat cataatctag caaatttggt
     1141 ttctcctctc gggaccagtt ggtccgcatt gctatcatta tatatagtta tataactata
     1201 tatgcataac attatattac cattctttct gttagtacac aatacaattc ttacaggttt
     1261 cttaacccga ttctgccatt tggtgaaata tattattatt cgtaaagtca cgtatcgata
     1321 gcatgaacta cactggtaaa tgaaaagggt ccgtagaagg acccttttga caaaatgtat
     1381 aaaaaagatc aatattcaaa tttccacata taccggcggt atgacattgg gtgcattcgt
     1441 atatccgcca tagccgcgat aaactgtttg gttaccggat ggattaataa gccattgaaa
     1501 tactcagcca ccgtttcatc gagctcgtca agtcccaatt ccgaggcgtc aatcacatag
     1561 tgatgtccgg catattgatc caggaatcgt tcgactcgat catccagcgg acgggtacgt
     1621 ccaacgcttt tcaacagcaa aatcgcaaga tctttatcgg ttgtctcaaa cgcgccgtgg
     1681 aaatattcac ccgtatgtac aggtacgcag tgcacagcct gcatctcctg aaagaagcag
     1741 aatgcgtcgg aatacgcgct ttcccatgcg gcgccactgg ccatgacatt ccaaaccgta
     1801 tcgtctttat aggtcattgc aaattttacc gcgcgttgtt ttaccttctt ataagctgca
     1861 tgatagattg cgggtaattt ttcaaaagcg tccatcgctt tatcataata tggataattc
     1921 tgcatttgat gaagcagttc aaaagcgatc ttcagcgccg tgccttggct gttctgatgc
     1981 atgagttcag gtttttcata ccattcatcg gaatggtaat agacaatcga ataatcggcg
     2041 ctttgcgcgg taagagaatc gccatagccg gtcaaaccga tcgtaattgc gcctgctttt
     2101 tttgcgacat tgagcgcctc taccgtttcg ggcgtcgctt tcgttgaaat gcaaattacc
     2161 aaagcgttcc gatccacgca cttcggcgtt gcgcttacaa actcactgct gttatatccg
     2221 gcagtgtgga acatggtgct ttcagcattt agcagatagc gggcgggata agacgccgaa
     2281 aacgagcctc cgcatgctgc aaagataact gttttaagcc ctccgcgtgc atccagttcc
     2341 tttttgacac agttaatagt ttcaatgacc tctttcattc ctgcaatcat agtccttata
     2401 tcctttcttc aagctctagc gaagctttgc tgtagcctgt ttatctattt gccagtcgcc
     2461 ttccggcgtc cggcagatta caacgccata gagttcacgt gctttctcga ccgatacttt
     2521 tttctcaata acatcattca aaaccttttc agccgctcgc atgcggggat tcccatatcc
     2581 tcccgcgccg ggagtgacaa cgcggatcac atcgcctttt tttaacggca ggcttgatgt
     2641 tttatgaccc atttttgttt ttgttccgtc ttttgaaatg cgccagaagg aaccgacttc
     2701 accatccagt ccgccttcaa gtccccatgg atgaaattta tggcgatccc ccagtccggt
     2761 gtaaagaatg ttgtcctgca agatttcaat ttcgcgttca attccaagtc cgccgcgatt
     2821 tttgccggcc ccacccgagt cttccttcag ttcatacttg cgagccagaa ttttattgaa
     2881 ttccagctcc gtcgcctcga taggcatatt ggacgtattc gtcatgttga cttgaacgcc
     2941 ggatagcccg tcataatgaa tggatgcacc gccgccgccc gcgattacct catggaatat
     3001 aaatacacga tcggacccga tctcgccttt tccggcaagg attgttgttg tgcaagcacc
     3061 gttcccggcg gtgacgacag cttccggcaa aacgggagcc agcgcaccaa agatcgcatc
     3121 gggcacgcgc tgcgaactat cgatctgcgc gccgacggga gcaggttcat tgggattcaa
     3181 tatgcttcct ttgggcgcaa taatgcgaat tgcgcgattg atcccctcgt ttgcaggaat
     3241 atccgggcca agcattgcct taatggcaaa gaaaacatcg gcctttgtgc agggccacgg
     3301 cacatttatc ggtgcttcta tctgggcggc cgaacctgtg aaatcaaccg ttaagtcatc
     3361 gtcgtcgatt gtaatcttcg cctttatcgg taatggatcg gggtatttat cgccgcagcc
     3421 atcaacgtaa tcggtatact cgtaatcacc attgggcagt ttggcaacaa cagcgcgcac
     3481 ccggcgttca gaataatcga tgatctcctt catacagtca atcaatacat ctccatagcg
     3541 tgcataggcg tcgcagatgc gttcgatacc gatcagattt gcggacatct gcgctgtcag
     3601 atcgccttcg cgttcctgtg ccacgcgggt gttgtccagc agcagatcca tcacgctctg
     3661 aataagcgcg ccattttcac aaatccgaat taccgggatg cgaatgcctt cctgaaacat
     3721 gctgtcggcg tcgccggagg tggaacccgg aaccttgccg ccaatatcgg aatgatgtgc
     3781 gatgttggca atccagccta caagcttgtc gcccgaaaat gccggggcgg caataacgat
     3841 atccggcaga tggttgcccc cgccatgata tggatcgttt gcaataaaca tgtcgccggg
     3901 tttgatgttt tctctgccaa atttcttata aatatttgga acgatgctga gcatggaccc
     3961 caataacgct gcaaggctct ccatctgcgc cacagaatta ccgtcagggt ctatgacggc
     4021 cgtgcataca tcatggcgtt ccttcatgtt ggtggaataa gcgctcctga taatgatgcc
     4081 gtaagtttcc tccgctatcg acatcagcag attgcggaca acttccacag tgaccgtgtc
     4141 aacccttctc ttctgcgtca tcttatttca cctcatcaaa atgaatcttt atattgccat
     4201 atccatctat gcgcgcggtc catccggnnn nnnnnnncca ccagcgttgt atccatctgt
     4261 tccatgatgc atggcccggt aatctcttga cccgcggcaa agtcggcgcg attataaacc
     4321 ggcgtatcaa ttcgcaatga atgattctgg aataacacac ttcgatgctc aatggcattt
     4381 ggaagcggat tgttcggccg caagggctct ggcgtgaaat tcggcttatc aatcgttccg
     4441 acagcagaga cgcgataact gacaaattgc acagctgccg ccttattgca atatccatag
     4501 ctgcgcttat gttccgcatg gaaatcctca atcgcctttg tcaacgcatt tacatccata
     4561 tctccagcgg aaattggaat acatatctca aaattctgcc ggtgataacg catatcaata
     4621 gaaaattcaa aactgcgtcg ttccgcgggc acgccttcgc ggtcaagcaa ttcagtgcct
     4681 tggcaaacca tactttcaaa ctgtcggttg acctcatcaa ggttttctac agttcccgcc
     4741 agaattcgtg tgcgcgacag gtcaaacttt gtgtcggcca tcagcaatcc cagagaacaa
     4801 agcgtccccg gatgtggcgg aatgattact tcgctgatgc ccagttcccc ggccacctcg
     4861 caggcatgca ggggaccggc gccaccgaat gccatcaggc tgaattcacg cacatcgtaa
     4921 cctttttcca cagagacgct tcgaatggcg cggatcatat tggaatttac aaccgaaatg
     4981 attcctttgg ctgcttcgtc ggaactcagg ctgcttttgg aacagatgtt ctcattgagt
     5041 gcgtcgagtg ccttttcaag atagacctgc atgcggcctc ccagaatgcg gctctgattg
     5101 agcttgccca gcaaaatatt ggcatccgta acaaccggac gtgtgcctcc tcgtccataa
     5161 catgccgggc caggcgttgc gcccgcactt tccgggccga ctttcagcgc gccgccctcg
     5221 tcaatccatg cgattgagcc gccgcccgcg ccgatggtaa taatgctgat catcggaatc
     5281 ctggccgggt agccttccac aagccgttcg ttggatacct gcggcgtgta attctcgatc
     5341 aagctgatgt cggcactggt tcctcccata tcaaatgtga tgatcttatc ggcttcgcat
     5401 tggcggccga tatacgccgc ggcaataacg ccggcactgg gtccggatac agctgttttg
     5461 attggacatt ctatggtttc ttttatcgaa ataatcgagc cgttggattg cgtaacatac
     5521 ggctctgcgg tgatgcccag atctttgatt gatttttgaa aattatatac atatttttcc
     5581 attacaggac ccaaatagct attcagcacc gtcgtactca ttcggctgta ttcgcggaat
     5641 tccggcgcca acttgctgga aatggtcaca tatgcctctg gatactcttc cttaataatc
     5701 tcttcaattc tcagttcgtg ggagggattg ataaacgaga ataacgtaca caccgcaatg
     5761 gattgaacct ttttacgttt cagttcacgc acaacctcgc gtacttcgtt ttcatccagc
     5821 ggcgtttcca cgcttccgtc atacaatatc cgttcgctga ccccatagtt caatcctgaa
     5881 gggatcagag ggcagggctt ctgttcctga agtttataaa gcgacgggcg gcgctggttt
     5941 ccaatctcca taagatcctt aaaccctttg gttgtgacca acgcgagtac cgcgcctttt
     6001 cgttcgatca gcgcgttcgt tcccaccgtc gttccatgcg caagatatgc aacctgcgcg
     6061 gacaatgcgt gtttgatcga caatatgtcg tcaatgccct ttacgatggc ccgagaggga
     6121 tcttccggtg tggagctgtc tttatagttg taaagctcgc cggtatcctg attgaaaacg
     6181 gaaaaatccg tgaatgttcc gccaacgtca accccgatca tatatcccat gtagtttcac
     6241 ctcacaaatt agtatcggcc ggtatatgcc gtgctaaaaa cttcttctat cgttatccgg
     6301 gaaaacggat tcaggttatc ccatggtctc tcaaccacgc cgcattcttt gcgtcggccg
     6361 cacgcgggtc cagccggtag cgcgcgtcat tgcattcaag cgaaaacaac cctccatacc
     6421 caagcgccag aatctcaccg ataatccgct ccagatccag atccccatcg cccagtgcca
     6481 tatggcagga tggatgcgta tcgtgaagat gcacatgtgc aagtttatcg cccagagccg
     6541 cgccatattc ggctacggtt tgccccatca cggtcatctg gccaatatca agcaatccaa
     6601 agacactgtt tgcgggcagt tgatcgatca tctctcgcac ttgcgcgggc gagtttacca
     6661 agtttgagga catcggcgtc agtgtttcgt aaactagctt tacgtcatgt gccacagcat
     6721 gtcgggcgat cttttcaatt gagtccaggc tgcgccgcag cgccggatct tttggcaagt
     6781 cgtaataacc actgcctgac gttatcaaaa cccaggggca ctgcagttcg gctgcaagat
     6841 cgactgctgt gcaataataa tccacactgc gttggcgcag cgaatcttcc tgtgccgcca
     6901 gattgaacgg ataattacat tgctccggcg tgatcgcgca taccgtcagg ttacgtgctt
     6961 tgagtttgga acgtaaatct ctgcaaaacg ccggtgataa atccatcaga tctatctgcg
     7021 gcgcggcggt ccagagttcc acttttgaaa aaccgagttg cgaagccgta tcgagaaaat
     7081 aatccatgga gtaacgatag tatggataac taccaatcgc tatccgatcc gcagagagtc
     7141 cagtcacgat accacctcct taatgcgtgt ttttagagaa gcaggaaccg gcgtaccgta
     7201 tccaaatgag ccatgaacaa ggcaggtttt ggaagcaaac gccgctgcat cggccagggc
     7261 tggcggtaag attttggcgc ggtaatttgc gtcagtccag cggcctttcc cgtcgctttt
     7321 cagtgcctgc gcaagcctga ccagcaaaca agccgcaaag ctgtcgcccg cccccatcgt
     7381 atcgattgcc tgcacagcgt atggtggctg tcgataaacc cgtgctccat cccatacgaa
     7441 agcgcccttg cttcctctgg ttgctaccac aacgccacaa ccttttttat acacgtgttc
     7501 gcaaagttcg aacgtttcat cttccgaaag ctcgctgcat gatagaaagc tgaaatcgat
     7561 ataagaacac acccgttcca gccggtcctg ttcgttccaa cggtaggaaa agtcatagct
     7621 gatcaacggt gacagcggtg ccagcagtgg aagctgctca tctataaatc cgttgttggt
     7681 cgtatgcacc acgtcgaaac aatcaatata agtaagatca tcttgaccca gcttaatagg
     7741 cgtcttttgc agcacgccgc ctttgttgct gcccagaaat acccggtctc catcgattag
     7801 cgtaacgcat gcaaagccgt tttcgccacg ggttcgccgg cagcggctat gtcccacgcc
     7861 taattcatca agcgtgcgca gcacatgttc cgccacttcg tccgttccgt taacccccag
     7921 aaaatcactt tgtacgtcga gttgtttcgc atacaccgca acattcaggg cttgaccgcc
     7981 cgggtacatc accccttggt tgagataaat atcacagaca ttgtccccga ggcctaacac
     8041 tttcaccata atattctctc cgctcagaaa gatgcttccg gctttaaatc ctcaggaata
     8101 gggcactcgt aagtcttatc cttcattgct tcggtaaact ctctgcgcac tgcgcgcatc
     8161 gcctcgccat cggtagccac ggcgataccg gtaagcgcca taaccttagc acctgtcagc
     8221 attccgttgt aacagagtgg ccttttaccc tgtgccacct cctgccagga gtgcccggga
     8281 gtatcctttg catagcaggc ggtcgtcagt tgtatcgtgg gagtaaacca actgacatcg
     8341 caaacgtccg tggaaccata ttttgcatgt ggtatcgctt tgtagggata cagcacgtcg
     8401 ttgataacct ttccacgaat ctgtgggatc agcggcgccg cttccggccc atataaatat
     8461 tccagggtgg aaaatgtgct ttcttcggca ttctccggga aagatttatg tatttcgcgg
     8521 cagaaatttt cgtcctccat cgtgtgcgga gttggtccga tttttacaaa ttgctcatag
     8581 gcaatccgtt cgagcgtacg atttggaatc agctccgaaa ggccgcctgt atattccaca
     8641 tccgcttcac agcgcgtcat catggccgca ccgtgtgcaa tctcgtcgat tctgcgggcg
     8701 gtttccatcg cccgtttatg attgtttgca cgaatgttat aaacaacttc cgaataattt
     8761 gggataacgt taggtgcaac gccgcccgca tcgataaaag catagttgat acaaacatca
     8821 cgctgcactt gaccgcgtaa ataatttgcg cctacgttca tcagctccgc cgcactcaac
     8881 gcgcttcgtc caatatgcac ctgcgaaggc gcatgagcgg agatgccttt aaagtggata
     8941 cgcaaactct gtatggccat aaaattcatc gaccaaatat tgttatcatc cgccggatgc
     9001 catgtgatgg cgcagtcaac atcgtcaaat aaaccggcgc gggccatata aattttaccc
     9061 ccgcctcctt cttcggcagg acagccataa aaccgaatcg tacccggcaa attgttttct
     9121 tcaagatagc gttttaccgt tattgctgcc gccatcgcac cggtgccaag aatatggtga
     9181 ccacatccat ggccgggcgc gccgggtgtg ataggactac gggtaacgga atccgcatgc
     9241 tgcgaaagac ccggcagagc atcatattca ccaaggaagc cgatcagcgg ccggccctgt
     9301 cccgcctccg cgacaaaagc ggtctgcatg ccggcaacgc cgtccgtaag gacgaattgt
     9361 tcatgccgca ggatggcttt caattcgtct gcggagcgct tttcatcgaa tctggtttca
     9421 gcgtaatccc agatgtgccc cgccgcttcc gtgtaacgat cccgattcga ctcgatggtt
     9481 tcaattacaa attgttcttt gttcataccc ttgctccttt cagctgttta aaggctggtc
     9541 caccaaatga cacattacaa aatgatcgtt cgccacctcc gtaaattgcg gcacttccct
     9601 tatgcaacgt tccgtggcat agggacaccg ctgatggaag tggcatcccg aaggcgggtt
     9661 ggctggatta ggtacatctc cttgcaagat gatgcgttca cgttgtaaag aaggatcggg
     9721 catcggaatt gccgacagca gcgcctgcgt ataagggtgc gtgggattct caaacaactc
     9781 ctttttctca gcaatttcaa cgattcgtcc gaggtacata actgcgatct ggttacaaat
     9841 atgattcaca accgacagat catgcgaaat aaagatatac gtcaggttga attcctgttt
     9901 cagttcatta ataaggttca gcacctgcgc gcggacggat acatccagcg cagagaccgg
     9961 ttcgtcgcat ataataagct ttggcatgac cgccaacgcc cgggcgatgc cgattctctg
    10021 ccgttgaccg ccggagaact catgcggaaa gcgagagata taggagtggt tgatccctac
    10081 catatccatc aactcaagaa ccttcatttc cttctctttt tcattcttgc cgctgaaaat
    10141 atccaatggt tcctgtatga tttcgcctac cgtcatgcga ggattcagag aggcgcccgg
    10201 gtcctgaaaa attatctgca tcttctgacg aagcagacgc atttcttcct tgcttaattt
    10261 aaggatgtcc cttccttcaa atagaatttc tccgtcggtt gcatcgatca gccgcaaaat
    10321 actgcgaccc aacgtggatt ttccacagcc ggactcaccc acgataccga aaatatcgcc
    10381 ctctttcaga gaaaagctaa ccccgtccac ggctcgcaca taccgtgttg ggccggaaaa
    10441 caaaccgcct ttagttataa aatatttctt taaattttta acctctaata ggttctgatt
    10501 catttcccgg cgtctccttt ttctcatact tgaagcagcg caccttacac ccgttcacct
    10561 cgtacagcgc aggcatttcg gccttgcagc gttccgtagc gtatggacaa cgtggccaaa
    10621 atctgcatcc cgtcggtaaa tcgtaaaggc tgggaacctg tccttctatg gtataaagct
    10681 ttttgttttc ttcacccatc ttgggtagtg cgcgcataag cccttgtgta tagggatgca
    10741 ggggtttttt gaataatttg tgcacctcgc tatattccat aaccattccc gcatacataa
    10801 caaggacata atcggccatt tccgccacaa cacccaaatc atgggtaatc attatgacgg
    10861 aagtgtccat tttcttctta agatttcgaa ttagatccag tatttgtgct tggatcgtca
    10921 catccagcgc agtggttggc tcgtcagcaa tcaacagttg cggatcgcag gccaaagcca
    10981 tcgcaatcat gacccgctgt cgcatgcctc cggatagctc gtgcgggtat tcatcaaccc
    11041 gtttttccgg tgccggtata ttaacaaggc gcagcatctc gatggacttt tccctcgcct
    11101 tttttttcga catcttttca tggcgcagga tcatttcacg aatctgatat cccacggtat
    11161 agaccggatt aagactggtc attggttcct gaaagatcat tgcgatttcc ttgccgcgga
    11221 cccgcgtcat atcctcgtcc gataagtgag taatgtcacg gcctttgaaa cgaatgttgc
    11281 cttccacaat cttacctttg ggtggcagca gatgcaaaat cgacatggag gttacactct
    11341 tcccacatcc ggattcccca acgatgccga gtgtttcatt cttatgcagg gtgaagtcaa
    11401 cgccatccac cgcgggaacc tgtcccttgc gatcgaaaaa ataggttttc aacccactga
    11461 cttccaacaa tacatcatcc ttcatagacg ccctcctgaa ccattttcat ttcgcaattc
    11521 cgtgtccgtt gtacgtgttt caaatttgga gcgatctttt taaatcctgc tgcatagctt
    11581 aaacctgtcc tttcggaaag gatatgatat gcaggacggt ctgcacggcc agacaaacgg
    11641 ccgtgcagcc gtcgcctcga atgtaccgct gaagcattac ttttttaatc cccgacattg
    11701 tttctcagtg tgtcgggata tctgcctgtc atcagtcggc gagttcccag gtccacactt
    11761 cgtagttgta ccaaataaaa tcatcctgcg ggatggatac caaacgggtc gagtgcgccc
    11821 acaggttgtt ctgatgatag agccagatgt atggctggtc ttccaccaaa gccgcctggt
    11881 aagcgtcaaa cgccgccttg cgctcgctga agcctaccaa agagctgccg gcttcggcca
    11941 gatcatacca tttggaatcg gttaacagtg aatagttctg ggcactgccc ggttttacaa
    12001 taacagcact ctcagcagga tcgatattgc ttccgccacc taacagtccg agatcaaaat
    12061 caccattgcg catcatttcc agaagagtcg ggaaatcata cgtctcaatt gtaacagtga
    12121 tgccaatatc ggccagattt tgctggatga taagggcgga ttgctcacgc acctgatttc
    12181 cttgcggaac gataagtcgg tattcttttg tgaaatcaaa tccggcggcc tccagttcgc
    12241 tctttgccgc ggtggggtca tagctgttgc ctaccaaatc actgttaaaa tacgggtgat
    12301 attcaggcat cggcccgacg gccacttgtc cctcgcctgc catcagctga tcgacgatca
    12361 attgcttgtc aattgccttg tcaaatgcaa cacgcaccgc ttgatcctgg aaatatgttt
    12421 caccttcaga aagattaaac tccatatact gataggaata accgggaaca gatgcggcaa
    12481 caagattgtc ggcggacttt acggtctccc agtcggaaag cggaatgctg ccggcaccga
    12541 ccacgtcgat atcgccgttc aacaatccgg cagtaagact gctggcggga actaccttga
    12601 cgaccagcgt tttaaaatcg ggacagccga gatagtaatc cgtgttggct tccagttcaa
    12661 gttcctcacc cgccgtgtcc ttcacgtatt tgaaggggcc gctgccaatc ggagccgacc
    12721 agaaatcgga cgtattgata tcagcatagt tacccgtgtc cagaatatgc tgaggcaaga
    12781 tatagaagaa gctgcacagg gagctaagga ttgtgctttg atcttttgcc gacttaaagg
    12841 ttagctgaag cgtgtattca tcaatcgctt ccgcacccaa cgcggcgggg tctgcgcaaa
    12901 cgccattctc gtcggtacca gcaagtatgc taaactcgct gcggtaatag ttgtcaacgg
    12961 cttcgttggt tactgcctgc agggtgaata ctgcgtcgtc ggcggtgagc ggttcaccgt
    13021 catgccaagt gcaattttca ttcatataga atgtaatgct cgtgttgtcc gcagacatct
    13081 cccacgaatt cgcaagccgt ggcagatatt cgccgtcatg attgcatgtg accatgcgct
    13141 caaacatttg gtcggcaacg cagtgtccat agttgccgga cgtattataa atgttcaagg
    13201 tgttccagga ggagatgatc gccatggtga cgacttcatc cttcggacct gtagcctcga
    13261 cggtgctgga tgtcgttgtc tccgtcgaag atgtagtttc cgaagaagct gtttctgaag
    13321 aggtcgggct ggtactgcaa ccaactagtg caagtggcag cactaaggca attgcaagaa
    13381 gaaagcatac ttttcttttc atgttctata ctcctttttt attttattta tcgcattcat
    13441 tcggcgatga atgtaataaa cttgatgctt agcggatcga agtcttggga tctaatgcgt
    13501 cgcgcaggcc gtttccaata aagttgatac tcagtatggt cgtcatcagc atcaatcccg
    13561 gaggcagcca catccacgca cgacttttca ggatcgtaat cgactgcgcg ttatacagca
    13621 tatttcccca agaggctgcg ggcggttgaa cgcccatacc cagaaagcta agtgacgatt
    13681 cgagaataat cgccgatgca atgcggaacg tgatcgatat aaaaatcggc gcaattgcgt
    13741 tgggcagaat atatttcgta atgacacggc tgtctttttc tccgatcgcg attccacctt
    13801 caacgtagtc tttttcacgc acgctcagca ctgtcccgta tatttgccgt gcaaattttg
    13861 tccatccgag cacgccgatg acgactgtaa ccgtgacgat agacggacca aacatagaaa
    13921 cgaggaatag aatcaaaatg gtggatggaa tggtcatgaa aatatcggac gcgcgcataa
    13981 ccatcgtctc cagagctccg cgatagaacg ccgccaaaag gcccagcgga atgccaatga
    14041 ttacactgat agcagtggac atgatgccca caaacagcga cacgcgcccg ccgtagagca
    14101 gtcttgctaa cacatcccgg ccgacatcgt cggttcccag cagatggtta ccacccgggg
    14161 cttgattaaa tgcggtcgcg tcgatctgcg actctgtaaa gggcagaaac gaaggcaata
    14221 tcaatacgac caacgcgatt aagataaagg taaaaaaacc aaataccgcc agtctgtttc
    14281 tgctatagcg tttaataaag ttgcggaatc ccgctttttt ttggctcaac ccagcacctc
    14341 cctatctggt tttattatcg atcagttgta ccggatccga ggatccagca ccccataaaa
    14401 caaatctgtc aatagattga acacaagcac agcgattgcg gtaacaatgg tgacacccat
    14461 tatgacaggg taatcgcgcg cgctgatgga tttaagcata agattgccaa gccccggcca
    14521 ggaaaaaact tgttctgtaa caacagcgcc gccaattaac atcggtattt ccatgccgag
    14581 ataagtggcc acaggaatca acgagttgcg cagcacatgt ttaaatataa cgacgcgatt
    14641 acgtaaccct ttcgaatacg ccgtacgcac ataatcttca cgcatgactt ccagcatact
    14701 gcttcgagtg tactgtatca ggctgccaac catggtgctt cccagtactg tggccggcat
    14761 aattagatga cgcagcaagg atagtattga agtgctttta cccgtatcgt acattccgcc
    14821 gatcggtaat attcccaatt gaaccgagaa caggtacaga aaaaccagtg caagaaaaaa
    14881 actcggagtc gaggttgata aaaacgaaaa aacggaagaa acattatccc aggctccatt
    14941 ttggtgacaa gccgactgaa tgccgagcgg aaacgaaatg atgcatgcga ccagaacggc
    15001 tgaaagcgtt agcagtaccg tcggccaaag cgcgttcata atcatgggca aaacttcacc
    15061 gctcgttcgg tatgaaatgc caagatttcc cgaaaggaag tttttcatcc ataacagata
    15121 ctgcatatag accggttggt tgaggcctaa tttctctgct tggcgcaata cctcggcttc
    15181 cgaaatgttt tggttgttga gcagaagctc cagtgggcta cccgaggcag tgtagcaaag
    15241 cccaaacacc agtatcgtaa tcccaataaa ggtcggaatc gcgattaaaa ttcttttcaa
    15301 cacatacctt ttcacattgc accacctaat tgagaaattc ggcgttttat acgcctcgta
    15361 cattttgtcg atgtttcgga gcatacataa aaatggcatc ctacacgttt cgtattttga
    15421 atcactcgca aacaacctcc aaagaaatct tcgcgtcgca atatttgcaa tttaattcct
    15481 atactgaacg tacgcacgaa tcattgctgc taaacattgc cccttttacg tagctatact
    15541 cttcatcgaa agcagagtaa ccggcaatcg tttcgttcaa aatcaaggca ggagttgttc
    15601 atgtgtgata gtccattgct tactcatctt atattgttat gttttgtatg tatttgtatt
    15661 atatccatat tgtattctgt tgtcaaccaa tattgttttt ttagaaagta ttgacaacca
    15721 tagaatagca atatatcaaa tcgaattgtc cgaaatagca atcgccaagc cgctatccgc
    15781 acaaaagtga gaggaaaacg aaatcaaccc agcctgttta ataaactact ctccaaagac
    15841 aggatatcag ttccggaaaa taccgacggc ataaaaaagc gctggtgaaa gggctccaat
    15901 gagttcctat cacacagcgc acttaaactt aagacgtgat gctggcttca aaacccgata
    15961 atcagatttt gaagccgctt aaaaatcgac ttttccactt tcgccctagc ctgacctcat
    16021 tggccgaatc cagtcttatt caaaacagtg gttcggccat gcagactcta tcatataagc
    16081 cgatgttgta tgagtcactc tccgatcgtc tcaacatata tacgtctcag ctgaggcccg
    16141 tcgaattcca caagatagat atcctgtgct ccgccgcaga taatttctcc gtcgatcagc
    16201 gggaaaacgc aatggtttcc ggtcagcgtg ctcttaatgt gaccggttga gttgggaata
    16261 caacacaatt tcggacagac tgttaagcaa cacatgtttg aagggctcga ttccagcgca
    16321 ttagccattg cataggactt aagtatccaa gatttcactt ctggagcttc gcaaagtagg
    16381 ccgctgcttt tttagcatgt cgcgctccat ttttaattct gcattttcaa gccggagcgc
    16441 cttgagttct tcctccattg tggccattgg cgttttctcg cctgacgccg tatatttctt
    16501 gcgccagttg tacaacaggt ttactgctat gcccagatcg tctgcgacgg ctcgcaccgg
    16561 ctttggactt gcataactta gtttgaccgc atttttcttg aattcatc
//
LOCUS       DAOSWR010000034        16526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2198_length_16526_cov_6.201263, whole genome shotgun sequence.
ACCESSION   DAOSWR010000034 DAOSWR010000000
VERSION     DAOSWR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2198_length_16526_cov_6.201263"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PK513_04055"
     CDS             <1..548
                     /locus_tag="PK513_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81657.1"
                     /translation="IHKGKISVQGTISGVVAGLIAITPAAGFVDFQGALIIGTAAGVI
                     CYLAVTYLKVLLKYDDALDVFGLHGVGGIVGAILVGVFANPEIGGAAGALYGNDKQLI
                     AQILSVVVTMVYSGVVTLILLLIIRTLMGLRVDPRVEYWGLDLAHHGETIVEYMEGLP
                     KKSPFNKSKSKPKAASKKAGD"
     gene            complement(559..1770)
                     /locus_tag="PK513_04060"
     CDS             complement(559..1770)
                     /locus_tag="PK513_04060"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019954671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOO81658.1"
                     /translation="MTVTFDDVQTAYKAIKDSTMRTPCVKAARLSLHLGIDLYLKLEN
                     LQHTNAFKARGALNKLLTLDEAQKKAGVIACSAGNHAQGVAYHAQRLGIPATIVMPSG
                     TPFNKIKRTEDFGAKVVLHGNNFDESVQFTLDMAKKEGLTFIHPFDDPLIVAGQGTLG
                     LEMLEQQPDLDVIVVPVGGGGLIAGVATAVKGVKPEIEVVGAQAEMFDAVKANLEKRP
                     GPVGGATLAEGIAVKAPSEENMKIIEKLVDRIDSASEAEIEDAVFDLLSSEKLVAEGA
                     PGAGLAVIKKNKKLYKGKKVGLVVCGGNIDSRLLSTLILRGLVRDGRICRLTFEIDDT
                     PGQLSDISRIIGESGANVIEVIHQRMMQSVSLKKAELEVVIEARDRHHVKEIVKTLRD
                     AGFKVKTDSDV"
     gene            2055..2795
                     /locus_tag="PK513_04065"
     CDS             2055..2795
                     /locus_tag="PK513_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO81659.1"
                     /translation="MSKKSRKPRNNKDTNIVHTAFDNRDWHPLDDEINGRRDQKYVKN
                     IKPRSDGQAALMKAIDEYSLTMAIGPAGTGKTYIAISKAAEALEKGQIERIILSRPAM
                     EAGESLGYLPGDLQEKMAPYLRPLFDSLGDRMGGKKVRQYIEDGTIEIAPVGFMRGRT
                     LNNAFVVIDEAQNCTYGQLKMLLSRLGWHSTMVVTGDPEQSDLLDGMSGLADISRRLE
                     TIDNIAICRLNETDIVRHPLVAEMLGAI"
     gene            2932..3465
                     /locus_tag="PK513_04070"
     CDS             2932..3465
                     /locus_tag="PK513_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81660.1"
                     /translation="MHTDGATSAHNEGATDLPEAMHSIHIMSAEMGGSGGPVYNYIIF
                     RDHSGADKVMMSGLAIERNDSDVRALRGVMAPPNEGGLPSEHFTIVKETEVFRGPVKE
                     YLRKMAMAVEALHFINAQNLNYRPEDENGDSPNSIAHTLIKAMGLEFPDETSAFWAPG
                     HERIILPKNWRSNYATT"
     gene            3452..3688
                     /locus_tag="PK513_04075"
     CDS             3452..3688
                     /locus_tag="PK513_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81661.1"
                     /translation="MLQPDLNDPIVKACVEIIQSAPQITPVTDEAAVSMCNKQRNFDP
                     ENPTVTADQIPALNANEDWNRAAGIVPKEMPSMR"
     gene            3775..4434
                     /locus_tag="PK513_04080"
     CDS             3775..4434
                     /locus_tag="PK513_04080"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOO81662.1"
                     /translation="MSEPFESPYMRLSDPSLWGIIFGNLVSMVLAITQDWPLGQIMWI
                     YWSQSVMIGVVNIIRMLSLESFTTKGMTMNDEPVPETEAGKRSVAIFFAIHYGIFHAA
                     YFAFLWQEQPLNALNMTEAMLMLLGLSAFVGSHSFSYMHNLRADFRQKKPNLGTLMFY
                     PYLRIIPRHLAIIFGSGIEAMGMLVFMGLKTFADAGTHMIEHYLFQKPGTDLRMKDLE
                     A"
     gene            complement(4462..4593)
                     /locus_tag="PK513_04085"
     CDS             complement(4462..4593)
                     /locus_tag="PK513_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81663.1"
                     /translation="MNTFQYTIIHELVDTNPTSEVGMVLKPVVKSHYACIDIKELQL"
     gene            complement(4626..4796)
                     /locus_tag="PK513_04090"
                     /pseudo
     CDS             complement(4626..4796)
                     /locus_tag="PK513_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(4856..4930)
                     /locus_tag="PK513_04095"
     tRNA            complement(4856..4930)
                     /locus_tag="PK513_04095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4896..4898),aa:Gly,seq:gcc)
     gene            complement(4993..5274)
                     /locus_tag="PK513_04100"
     CDS             complement(4993..5274)
                     /locus_tag="PK513_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81664.1"
                     /translation="MNIELGILNDGGVVLVSDNPLPEVVRRVEYYRDQRIFMLVYNND
                     DEGQLMDYEIPEDMSHPIEKSPNVIIYTMFKNHDPIGYRVPLIKVGELF"
     gene            complement(5271..5594)
                     /locus_tag="PK513_04105"
     CDS             complement(5271..5594)
                     /locus_tag="PK513_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81665.1"
                     /translation="MSDTVESDAHAAEALIAELQACGNEPVNNAWMAGRMPPGQCLGL
                     HPAPKDGQPGIREVYVTNGDMFKELVEKLPEAMANPRMAFCQVAGTEAPQQNADLTLD
                     PRMLL"
     gene            5733..6251
                     /locus_tag="PK513_04110"
     CDS             5733..6251
                     /locus_tag="PK513_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOO81666.1"
                     /translation="MNSLDFPLSPLPTTPENLFNALKNLDISYALYHHQAVFTVAESD
                     TLDRDIPGVHCRNLYLRDKKKKNFLVVAANETAVDLKTLQSKLNCGRLSFGSPDRLWE
                     FLGIRPGSVCPFTVMNDPEHRVQIVLDARMMAAETVNYHPLDNRMTIGLTPADLLKFL
                     GHTGHQPIILDL"
     gene            6298..7242
                     /locus_tag="PK513_04115"
     CDS             6298..7242
                     /locus_tag="PK513_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HOO81667.1"
                     /translation="MFGSKPRNIQPSAVRAPGAQQETIFDVGTADFETKVMAASMEKP
                     VIVDFWAPWCGPCKQLMPVLERAVQAAGGEVLLAKVNLDENPELAGALRVQSVPTVFG
                     FWGGQPVDAFQGAQPESAVQSFIDKLVQAARAARPDAIDIPEVLKIAAEALGASDLQT
                     AQSAYAQILQQDEKNAHAYAGMIRTFIAAGMIKQAQGLVAGAPEEITKASEFEAALTA
                     LELAQNVPSSDELLALEKKAADNPEDLDAKCALAEVQFGADQKEQAIETLLAAIAQDR
                     EWNDQEARKTLLRFFEALGHSDPLTIAGRKKLSRLLFS"
     gene            7269..7934
                     /locus_tag="PK513_04120"
     CDS             7269..7934
                     /locus_tag="PK513_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOO81668.1"
                     /translation="MEHNPFAPQFDDLPQSLAVFPLSSAFLLPSGHLPLNIFEPRYLQ
                     MVEDALAGNRLIGMIQPQPHQQDQERPALVKTGCAGKIVEFSETTDGRYLVNLCGIYR
                     FDVTEEISTPKAYRVVKPDWTPYKGDVSAHRCLDLDREKLKALLHNYFDQHGMDCDWG
                     MLDEAPDGRLITCLSMVCPFEQAERQALLEAPCCKSRAQIFMSMLEMAVRSGKEPHSC
                     YKH"
     gene            7967..8146
                     /locus_tag="PK513_04125"
     CDS             7967..8146
                     /locus_tag="PK513_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOO81669.1"
                     /translation="MSAKIDPKLLEILVCPLAKSPLRYDKEAQELVSEQAKLAYPIRN
                     GIPIMLVDEARALDS"
     gene            8356..9939
                     /locus_tag="PK513_04130"
     CDS             8356..9939
                     /locus_tag="PK513_04130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO81670.1"
                     /translation="MTRAFDKVQADPGRIIIFDTTLRDGEQSPGCSMNLEEKLKMAVL
                     LDELGVDVIEAGFPIASPGDFESVNEIAKVVKNATVAGLCRAKQADIEAAGNAIKPAE
                     SRRIHTFISTSPLHMKYKLKMGPNSVIEAVHESVALARGFTDDVEWSAEDASRTEDDF
                     LCQCVETAIAAGATTINIPDTVGYAVPEEYGRQIKMLFERVPNIDKAIISTHCHNDLG
                     LAVANSLAGVQNGARQVECTINGIGERAGNAALEEIVMTLRTRADALPYKTGIETTRI
                     TKTSRTLSTITGFSVQPNKAIVGANAFAHASGIHQDGMLKNNQTYEIMTPESVGLNES
                     KLVMAKHSGRAAFKNKLQELGFDLGEQAFQDAFVRFKSLADRKKEIFEDDLIALVEDE
                     VIRENETVRFVSLQVQAGSQGPQIAELCVKIDGEEKCARVEGNGPVDAIFKAIREILP
                     HEGARLQLYQVHAVTGGTDAQAEVTVRLEEDGKTVNGQGADADTLVASARAYIHALNK
                     LLTKRESMNPQTAGEALAP"
     gene            complement(10021..10644)
                     /locus_tag="PK513_04135"
     CDS             complement(10021..10644)
                     /locus_tag="PK513_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO81671.1"
                     /translation="MKTPDLNCRFCPRLFEFRAQNKDKFPDKFNAPVPSFGDLNAQVL
                     VVGLAPGLKGANFSGRPFTGDYAGDLLYATLLKFGFARGEYARHKDDGLELVDCRVTN
                     AVRCVPPENKPTTDEMNTCRDFLKSEIAAMPNLRTILSLGLISHNAVLKIFGHKISAF
                     KFAHGAHHHLNDRFTLVDSYHCSRYNTQTKRLTTEMFETIFSSLRGV"
     gene            10754..11158
                     /gene="rpoZ"
                     /locus_tag="PK513_04140"
     CDS             10754..11158
                     /gene="rpoZ"
                     /locus_tag="PK513_04140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOO81672.1"
                     /translation="MARVTVEDCIDKVDNRFELVMVAAQRARKIGSGAALTIERENDK
                     NTVVALREIAEQTVEVETLKEELVRSHQRMIAYEEEDESIDLMDGEEEWGTIASNHGK
                     AGASGMYAEEDIAQDDEPSLEDMAGGAPAEEE"
     gene            11306..13465
                     /locus_tag="PK513_04145"
     CDS             11306..13465
                     /locus_tag="PK513_04145"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOO81673.1"
                     /translation="MTDLPPLVDKIKSYNPDFNPQIVEAAIAFAKDKHEGQTRSSGEP
                     YYTHPVAVASILAEMKLDLGSILTAILHDTIEDTDATYDDLKDKFGCEVADLVDGVSK
                     LTRIESKTVEGKQAENFRKLLLAMSEDIRVLLVKLADRLHNMRTIEGIKNPEKRRRIA
                     LETLEIYVPLAERIGVHKIKGELEDLAFSILNPEARESIAKRLAFLRAEGTDLVDDII
                     AELKKSLEDVGIKAEISGREKTGYSIWKKMQRKNVAFEQLSDIMAFRIVVGSVEQCYA
                     TLGLIHSHYPSIPGRFKDYISTPKPNGYRSIHTTVMGPARQRIEIQIRTSEMHYQADM
                     GVAAHWAYKQGGQPMVQDVKKYRWLRELLEILEQEQQPEDFWENTKLELFQEHVFAFS
                     PKGDLIELPSGATPIDFAYAIHSDIGDRCVGAKINGRIAPLNTRLANGDQVDIITSKT
                     QQPSPTWERFVVTGKARSHIRKFVRNRQRNEYVTLGKAMLQKIFRQEGYEFSEKGVSG
                     ILKQFKAEENDDIYARIGSGTLVARDAFKAIFPAHKTAIAKRPSADMDQAVMVRPLSG
                     SDAPMPIKGLIPGMAVHFARCCHPLPGDRIVGIVTTGKGVTIHTIDCETLETFADTPE
                     RWLDVSWGEGPDSPEAHVGRINITLQNEPGTLATLSTVIASNGGNITNLKITSRSVDF
                     WQILVDVYVNDTKHLSNIIAALRATPIISSVERAKGQ"
     gene            13469..15265
                     /locus_tag="PK513_04150"
     CDS             13469..15265
                     /locus_tag="PK513_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOO81674.1"
                     /translation="MYDILSQIAWIAFFSIAAQWLGWRLKTPAIVFLLIGGFLAGPTL
                     NLVQPETLLGDLLNPVISLAVGIILFEGALNLNFKEIREARASIRQVILVGAPVAWLL
                     TTLAGHYVAGLSLPVALTFGALLIVTGPTVIMPLLKNAHLKERPASILKWEGIINDPI
                     GAVLAVLCYEFFRLQALENVALSEFFTSTVLAIIMITFFGVAIAYSLSWLFNHDVVPE
                     YLKPTALLSTALIYFVICNSIEHDFGLIGVTILGVALANMGISSIEELKKFKETISIM
                     LVSGVFILLTTKIDPAILFSIDWRGFAFIAMLLFVIRPLTLLASSIGTKMSWQEVILT
                     GWIAPRGIVCAAVAGVLGPYMVGIGYQDGEQILALAFAIVLFTVFAHGLSAKPVAQKL
                     KLAYTGKDSLIIVGASEWAVQFAKMLKGRDVDVMIADKNWHALREARLSDIPTYYGEI
                     LSEETEYHLELARYNTLLAVSNNPAYNALICNMFIHEFGRDKVYQFLPHEENEHERRK
                     IAHTIRGRTFGQADMDYWDIAGDYMQGWRFRASRAGQEENRIQFQQRIEEGNLKIVGY
                     ITQKGRLDLRQPESLEKFEEQDLLIFFEQGDS"
     gene            complement(15291..15806)
                     /locus_tag="PK513_04155"
     CDS             complement(15291..15806)
                     /locus_tag="PK513_04155"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOO81675.1"
                     /translation="MNIQDHIRTIPDFPKPGINFYDIATLLANPSAWRETIARLHRLT
                     EDYQPDLLMGIESRGFLVAAPLAYEMGCGFGMVRKHGKLPGQTLAHGYALEYGEDIIE
                     IQPDLIPAGSKVVLVDDLLATGGTMQAAEILLHKAGAHVVANLCIIELEGLNGRDKLT
                     APLESVIRCPA"
     gene            15888..16472
                     /locus_tag="PK513_04160"
     CDS             15888..16472
                     /locus_tag="PK513_04160"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOO81676.1"
                     /translation="MFKRRTPRTVLQNIRELFWPSMGWVRAAHYTKHRVVRLSDSTQK
                     IAFGLAIGVAISFTPILGTHFVQAGFLAYVLRANFLAALIGTFAGNPWTFPFMWWAAI
                     SLGSFIFQLLGLPAEAALPEHADFWVIWDLIKSEPYRIFAPWFVGGYLLGALTVPLTY
                     SLFYRLVGSAQAARKKARENRLHKVAREVTGQKK"
BASE COUNT         4139 a         3841 c         4254 g         4292 t
ORIGIN      
        1 tgattcataa aggaaaaatc agcgtgcaag gtacgatctc aggcgtagtg gccgggctta
       61 ttgccattac tccagcagca ggattcgtcg attttcaggg agctttgatt attggcaccg
      121 ctgctggtgt gatctgttat cttgccgtga cctatctcaa ggtattgctg aaatatgacg
      181 acgctctcga tgtttttggc ttgcacggcg ttggcggcat tgttggtgcg atcctggttg
      241 gcgtgtttgc caacccggag attggcggcg cggcaggcgc actttacggc aatgataaac
      301 agcttatcgc gcaaattctg tctgtcgttg ttactatggt ctattccggc gtggttacgc
      361 ttattctgct gctgatcatt cgcacactga tggggctacg ggttgatccg cgcgtagaat
      421 attggggact tgatctggcc caccacggtg aaaccattgt tgaatatatg gaaggtttgc
      481 cgaagaaaag cccgtttaac aaaagtaaaa gcaagcccaa ggccgccagc aaaaaagcag
      541 gtgactaaaa gcaggtgact aaacgtcgct atcagttttg accttgaagc cagcatcgcg
      601 cagggttttg acgatctctt taacgtggtg gcgatcacgg gcttctatga caacttcgag
      661 ttcggccttt ttcagggaaa cgctttgcat catgcgctgg tggataacct cgatgacgtt
      721 ggcgccagat tcaccgatga tgcgggaaat atcggaaagc tgacccggcg tatcgtcaat
      781 ttcgaacgtt aagcggcaaa tacgaccgtc acggaccagc ccgcgcaaaa taagcgtgga
      841 aagcaagcgc gaatcgatat tgccgccgca gacaacaagg ccgacttttt tgcctttgta
      901 gagctttttg tttttcttga tgacggctag cccggcgcca ggcgcacctt cggcaaccag
      961 tttttcagag gaaagcagat caaaaacagc atcttcaatt tctgcttcgc tggcagaatc
     1021 aatacgatca acaagttttt caatgatctt catgttttct tctgagggcg ctttcactgc
     1081 aatcccctca gccagcgttg cgccgccgac agggccgggg cgtttttcga ggttcgcctt
     1141 gaccgcgtcg aacatttcgg cctgcgcgcc gacaacctca atttcaggct ttacgccttt
     1201 gacagctgtc gccacccctg cgatgaggcc gccgccacca acagggacta cgatgacatc
     1261 aagatcaggc tgctgttcca gcatttccag tcccagcgtc ccttgccccg ctacaatcaa
     1321 cgggtcgtca aacggatgaa tgaaggtcag gccttctttt ttggccatgt ccagtgtgaa
     1381 ctggacagat tcatcaaagt tattgccgtg aagtacgacc tttgctccga aatcttctgt
     1441 tcttttaatt ttgttaaaag gcgtgccgct tggcataaca atagtagcgg gaatgccaag
     1501 acgctgcgca tgataggcaa ccccttgggc gtggtttccc gcgctgcaag caatgactcc
     1561 cgcttttttc tgtgcttcgt caagggttaa aagcttattc agcgcacctc tggccttaaa
     1621 tgcattggta tgctggagat tttccagctt aagatacagg tcaatgccta aatgcaaaga
     1681 caagcgtgcc gccttaacac aaggtgtgcg catggtggag tctttgattg ccttataggc
     1741 ggtctgaacg tcatcaaatg ttacggtcat agttaaagct cttttctttt aagcaggtga
     1801 caataaaagc ctatgtgggg aatagctagt cttttttttg tgatcaaaac cggaaataca
     1861 tggcgaaagc cgcagaaatt tggtactgtt agagtgtctt ggtaactata aggacttctc
     1921 cttgatcgca gtttaccttc ttcagaaata taaaccatct ggcgcttttc aacagatcgc
     1981 cccaaattct ttaatctctc ctttttattt gttttttaaa gccctcccca cccccatgga
     2041 aaggaccatc acgcatgtcg aaaaaatccc gcaaaccccg taataacaaa gataccaata
     2101 tcgtccacac tgcttttgat aatcgagatt ggcacccttt agacgatgaa attaacggcc
     2161 gccgggatca aaaatatgtg aagaatatca agccgcgatc tgatggacag gcggcgctta
     2221 tgaaagcgat tgatgaatat tcgttgacga tggccatcgg cccggcagga accggaaaga
     2281 cctacattgc gatttccaag gccgctgaag cccttgaaaa aggacagatt gaacgcatta
     2341 tcctctctcg cccagcgatg gaagccggag aaagtctggg ctatctaccc ggtgacttac
     2401 aggaaaagat ggcaccatat ttgcgcccct tgttcgattc attaggggac cgaatgggcg
     2461 gcaagaaggt gcgccagtat attgaggatg gcacgattga gattgccccg gttgggttta
     2521 tgcgcgggcg tacactgaac aacgcctttg ttgttattga tgaagcgcag aactgtacgt
     2581 atggacagct aaaaatgttg ctttcccgtc tgggctggca ttcgaccatg gttgtgacgg
     2641 gcgatcctga acaatcggat ttattggatg ggatgtccgg gctggccgat atttcacgca
     2701 gattggaaac cattgataac attgcgattt gccggttgaa tgagacagat attgtccgtc
     2761 atccattggt ggcggagatg ctgggcgcaa tataaggtat aaattcctaa gtttattctt
     2821 ctccttctct aaaaggccgg ttttgtactg gccttttttg tgtctctttt accgaaaatt
     2881 aactcccgta gcgcacacta agtaggtgaa aataaatgag ggtttaacgc aatgcataca
     2941 gatggggcga caagtgcgca taatgaaggg gctacggatt tgcccgaagc catgcattcc
     3001 attcatatta tgagtgctga aatgggcgga tcgggaggcc ctgtttataa ttatatcatt
     3061 ttccgggatc atagcggcgc agacaaagtg atgatgagcg gtctggcgat tgaacgtaat
     3121 gacagcgatg tgcgggcgct gcgcggggtt atggctccgc cgaatgaagg cggcctgccg
     3181 agcgagcatt tcacgatcgt gaaagaaacc gaggtgtttc gcggcccggt gaaagagtat
     3241 ttgcgcaaga tggcgatggc cgttgaggcg ctgcatttca tcaatgcaca aaatttgaat
     3301 taccgtccgg aagatgaaaa cggcgatagc ccgaattcga ttgcccatac gctgatcaag
     3361 gcgatggggc tagaattccc ggatgagacg tccgcgtttt gggcgccggg ccatgaacgc
     3421 atcattttac cgaaaaactg gagatcgaat tatgctacaa cctgatttaa atgaccctat
     3481 tgttaaagct tgtgtagaga ttattcagag cgcaccacaa ataacccccg ttactgatga
     3541 ggcagctgtt tccatgtgta ataaacaaag aaacttcgat ccggaaaatc caaccgtcac
     3601 ggctgatcaa attcccgcct taaacgcaaa tgaagattgg aacagagccg ccggaattgt
     3661 gccgaaggaa atgcctagta tgagataaca ataaagttta aaccacggaa aacgaacggg
     3721 tctgctgtga gcagacccgt tttttcgtgg gccattatct gataaggtca tgcgatgagt
     3781 gagccattcg aaagcccgta tatgcgtctg tctgatccgt ctctttgggg aattattttc
     3841 ggcaatctgg tttctatggt tctggcgatt acgcaagatt ggccgctggg ccagatcatg
     3901 tggatttact ggagccagag cgtgatgatc ggcgtggtca atatcatccg gatgctctca
     3961 ttagagagct ttaccaccaa aggcatgacg atgaacgatg aacctgtgcc ggaaaccgag
     4021 gcaggaaagc gttctgtggc gatatttttt gcgattcatt acggaatttt ccacgcggcc
     4081 tattttgcgt ttttatggca ggaacagcca ctaaatgcgt tgaacatgac cgaggcaatg
     4141 ttgatgctgc tgggcttaag cgctttcgtt ggctctcata gtttttccta tatgcataat
     4201 ttgcgcgcgg attttcgcca gaaaaagccc aatctgggga cgttgatgtt ttacccgtat
     4261 ttgcggatta tcccgaggca tctggcgatc attttcggca gcgggatcga ggctatgggg
     4321 atgctggtgt ttatggggct gaaaacattc gccgatgcgg ggacgcatat gatcgagcat
     4381 tatttgtttc aaaagccggg gacggatttg cggatgaagg atttggaggc atgagcgagt
     4441 tgacgatcaa taacctcatg cctatagctg tagttctttg atgtcgatac aagcataatg
     4501 gcttttgacc actggcttca aaaccattcc gacttcgctt gtcgggtttg tatcaacgag
     4561 ttcatgaata atggtgtatt gaaatgtatt catacaatat tgccgtcctc agtgcacttg
     4621 ctatcagctt ctttcaaaga aatttcggga tagacaccca aagccaatcg tttctcttta
     4681 tatttatcac ctgctttaat gcggtatttc atgcgccaat attttgagcc gttgggcatg
     4741 acctcgagat atagcccacg cccagccgat aatttatgct gtcctacgga ccgcataaaa
     4801 aagccctgaa aatcaatgtt tttcgggctt tattggacta tattgggtga taaaatggag
     4861 cgagcgaagg gattcgaacc ctcgaccccg accttggcaa ggtcgtgctc tacccctgag
     4921 ctacgctcgc atcgtttggg aatagatttg aagatttata gaggttttgt gacagaacgc
     4981 aaggtcaaaa atctaaaaaa gttctccgac cttgatgagc ggcactctat agccgatcgg
     5041 atcatggttt ttgaacattg tgtaaatgat tacattcggg ctcttctcaa taggatgaga
     5101 catatcttcc ggaatttcgt aatccattaa ttgcccttca tcatcgttat tatagaccaa
     5161 catgaatatc cgctgatcac gataatattc aacgcggcgt accacttccg gcaacgggtt
     5221 atcactcacc agaacaacac caccatcatt gagaatgccc agctctatat tcataacagc
     5281 atccttggat caagagttaa atctgcgttt tgctgaggag cttctgtgcc tgcgacctga
     5341 cagaaagcca ttctcgggtt agccattgct tcaggaagct tttcaaccaa ctctttaaac
     5401 atatcaccat ttgttacata gacttcacgg atacccggct ggccatcttt cggggccggg
     5461 tggagcccga ggcattgccc cggcggcata cgtccggcca tccacgcgtt attgacgggt
     5521 tcattcccac atgcctgtaa ttctgcaatt aaagcttctg ccgcatgggc atcgctctct
     5581 accgtatcac tcattttccc tatccacaca ttatttttta ttatttgctg tgtttgtgct
     5641 tattatgata agtgaaaaaa cttaagaaat tgcttatata tgaaaacaat ttatctaaaa
     5701 ttttatctat ttttatatgt aggaaaggac gtatgaatag tttggatttt ccattatctc
     5761 ctcttccaac cacgcccgaa aaccttttca atgcattgaa aaatctggat atttcttatg
     5821 ccctttatca tcaccaagcc gtctttacgg ttgcggaaag cgatacgctc gaccgtgata
     5881 ttccgggtgt gcattgccgc aatttgtatc tgcgggataa aaagaagaaa aattttctgg
     5941 tcgttgcagc caatgaaaca gcggtggatt taaaaacgct acagagcaaa ttgaattgcg
     6001 ggcggctatc ttttggttcg ccagatcgtt tatgggaatt tttgggtatc cgccccggtt
     6061 cggtttgccc attcaccgtg atgaacgacc cggagcatcg tgtgcaaatt gtgcttgatg
     6121 cacgtatgat ggcggcggaa accgtaaatt atcacccttt ggacaatagg atgacgattg
     6181 gccttacgcc tgcagatttg cttaaattcc tgggccatac aggccatcaa cctataattc
     6241 ttgatttata gggacaaaag cgtacataaa gggctgttat aatgaggatt aaaaagcatg
     6301 tttggatcaa aaccgcgaaa tattcagccc tctgctgtgc gagcacccgg tgcacagcaa
     6361 gaaacgatct ttgatgttgg caccgccgac tttgagacca aggttatggc cgcatcgatg
     6421 gaaaagccgg tgattgttga tttctgggcg ccatggtgtg ggccttgtaa acagcttatg
     6481 cccgttttgg agcgtgccgt acaggcggct ggtggcgaag ttttactggc caaggtcaat
     6541 cttgatgaaa atcctgagct ggcaggcgcc ttgcgcgttc aatccgtgcc gaccgttttt
     6601 ggtttttggg gcggacagcc ggttgacgct ttccaaggtg cgcaacctga aagcgctgtg
     6661 cagagcttta ttgataagct tgtgcaagct gcgcgcgcag cacgtccgga tgcgattgat
     6721 attccggaag ttcttaaaat tgctgccgaa gcgctgggcg caagcgatct gcaaacggca
     6781 caaagtgcgt atgcgcagat tttgcaacaa gacgagaaaa atgcgcacgc ttatgccgga
     6841 atgatacgca cttttatcgc cgcaggaatg attaaacagg cgcaaggatt ggtggccggc
     6901 gcaccggaag agattaccaa agcttccgaa tttgaagcag cactcaccgc gcttgagctg
     6961 gcgcaaaacg tcccttcatc tgatgaactg ctcgctttag agaaaaaggc agcagataac
     7021 cccgaggatc ttgatgccaa atgcgctctg gctgaggttc agtttggtgc agaccagaaa
     7081 gagcaagcga ttgaaacgct actggcggcg attgctcaag accgggaatg gaacgatcag
     7141 gaagcccgaa agacgctgct acggtttttc gaagcccttg gccatagcga tccgcttact
     7201 atcgcgggac gtaaaaaatt atccagactc cttttttctt aagaattgcg gactatttaa
     7261 tttgagttat ggaacataat cctttcgcgc cgcaatttga tgatcttccg cagagtctgg
     7321 cggtctttcc gctttccagc gcgtttttgc tgccttccgg gcatctgccg cttaatattt
     7381 ttgaaccacg ctatctgcaa atggttgaag atgcattggc tggcaatcgc cttatcggga
     7441 tgatccagcc ccagcctcat caacaggatc aggaaaggcc ggcgcttgtt aaaaccggct
     7501 gcgcgggaaa gattgttgag tttagtgaaa caaccgatgg gcgctatctt gtcaatcttt
     7561 gcggcatata tcgctttgat gttaccgaag agatcagcac acctaaggct taccgcgttg
     7621 tgaagccgga ttggacgcca tataagggtg atgttagcgc gcatcgctgt cttgatttgg
     7681 accgggagaa gcttaaagcc ttgctgcata attattttga ccagcatggc atggattgtg
     7741 actggggtat gctcgacgaa gcgccggatg gacggcttat cacctgctta tcaatggttt
     7801 gtccgtttga gcaggcagag aggcaagctc ttctggaagc gccttgttgt aaatcgcgcg
     7861 cgcagatttt tatgagcatg ctggaaatgg ctgttcgctc cggtaaagag ccgcactctt
     7921 gctataagca ttgatttatc tattcctgcg cgctaaagct gtaattatga gcgctaaaat
     7981 cgatccaaaa ttgttggaaa ttctggtttg tcctctggcc aaaagcccct tgcgctatga
     8041 taaagaagcg caagagcttg tttccgagca ggccaagctt gcctatccta ttcgtaacgg
     8101 cattccgatt atgctggttg atgaagcgcg ggcgctggat tcataaaaaa acttgcatat
     8161 cctcgtaaaa atccgtttat atacgctcat gagcaacgtg caaacacaat tgctgctaga
     8221 ccttctgctc cttatgcgcc cctgagcgat gcatgaggca ttttatgtgc ctgcgctaca
     8281 ggggaaaaat tccagacatt gaaatattaa gacaagcgca ttaaaaagaa gcgcatattt
     8341 aaggagtata cgcacatgac ccgtgctttc gataaagtac aagccgaccc aggccgtatc
     8401 attattttcg atacaacgct gcgcgatggt gaacaatcac cgggctgttc aatgaatctg
     8461 gaagaaaagc tcaaaatggc cgtgctgctt gatgagcttg gcgtggacgt gattgaagcg
     8521 ggcttcccga ttgcctcgcc gggcgacttt gaatcggtca atgaaatcgc caaggtggtt
     8581 aagaatgcca ccgttgccgg attgtgccgc gctaagcagg ccgatattga agcggcgggc
     8641 aatgcgatca aaccggcgga aagccggcgt attcatacct ttatctccac cagtcctttg
     8701 catatgaaat ataagctcaa aatgggtccg aattcggtaa tagaagccgt gcatgaaagc
     8761 gttgcgcttg cccggggctt tactgatgat gtggaatgga gcgcagaaga cgccagccgc
     8821 acagaagatg atttcctgtg ccaatgcgtt gaaacggcga ttgccgccgg ggcgaccacg
     8881 atcaatattc ccgatacggt gggttatgct gtccctgaag aatatggccg ccagatcaaa
     8941 atgctgtttg agcgcgtgcc caatatcgac aaggcgatta tttcgactca ctgccataat
     9001 gatttggggc tggctgtggc gaactcgctg gcaggcgtac agaacggcgc acggcaggtt
     9061 gaatgcacga ttaacggtat tggcgagcgc gcggggaatg cggcgctgga ggaaattgtc
     9121 atgactttgc gcacccgcgc cgatgcgctg ccctataaaa ccggaattga gaccacgcgc
     9181 attaccaaaa cttcgcggac gctttcgacc atcaccggct tttcggtgca gccaaacaaa
     9241 gcgattgtcg gggcgaatgc ctttgcgcat gccagcggca ttcatcagga cggtatgctt
     9301 aagaacaacc agacctatga gatcatgacg ccggaaagcg tcgggcttaa tgaatccaaa
     9361 ctggtgatgg cgaaacactc aggccgggct gcgtttaaaa acaagcttca agagctgggc
     9421 tttgatctgg gtgagcaagc ctttcaagac gcgtttgtgc gttttaaatc gctggcagac
     9481 aggaaaaagg aaatctttga agatgacctg attgctttgg ttgaggatga ggttataaga
     9541 gaaaacgaaa cggtacgttt tgtatcgtta caggtgcagg ccgggtctca gggtccgcaa
     9601 attgctgagc tttgcgttaa aatcgatggc gaagagaaat gcgcgcgtgt ggaaggtaac
     9661 gggcctgttg acgctatttt caaagctatt cgcgaaatac tgccgcatga gggcgcccgg
     9721 ctgcagcttt atcaggttca tgcggtgacc ggcgggacgg atgcgcaggc cgaggtcacg
     9781 gtgcgtcttg aagaagacgg caagacggtg aacggtcagg gcgcggatgc cgatacgctg
     9841 gtggccagcg cgcgggcgta tatccatgcg ctgaacaagc tgctgaccaa gcgcgagagc
     9901 atgaacccgc aaactgcggg cgaagccctt gcgccttgat ttcaatatca ttgcgagcga
     9961 ctgaaaggag cgcggcaatc cagttttcac cctaacggac acgaatggat tgcttcgtcg
    10021 ttacactcct cgcaatgacg agaatatcgt ttcaaacatt tccgtcgtca gacgttttgt
    10081 ttgggtgttg tagcgtgagc aatgatacga atcgacgagt gtaaaccggt catttaagtg
    10141 atggtgcgcg ccatgggcaa acttaaaagc cgaaatttta tggccgaata ttttgagcac
    10201 cgcattatgc gagatcagcc ccagtgaaag gatggtgcgg agattgggca tcgcggcaat
    10261 ttcactttta aggaaatccc ggcaggtatt catctcatct gttgtcggtt tgttttccgg
    10321 tgggacgcag cgcaccgcgt tggttacacg gcaatccacc aattccagtc cgtcatcttt
    10381 atgcctggca tattccccac gcgcaaagcc aaatttcagc aaggtcgcat acagcaaatc
    10441 cccggcataa tcgccggtaa aggggcgccc ggaaaaattc gcacctttaa gccccggcgc
    10501 gaggcctacg accagcactt gcgcgttcag atcgccgaaa gacgggacgg gtgcattgaa
    10561 tttatcgggg aatttgtctt tattctgagc gcggaactcg aataaacgtg ggcaaaaccg
    10621 gcaattcagg tcaggggttt tcatcgcccc atcatcgtca atatccctga aacgtcaagg
    10681 ggcttgcttt taaactcaaa actgcctata tatcggcctt caagttctga aagtttagat
    10741 gaggaagtaa cgaatggccc gcgtaaccgt tgaagattgt attgataaag ttgataaccg
    10801 ctttgagctt gtgatggtcg cggcgcaaag agcgcgtaaa atcggctccg gcgccgctct
    10861 gacgattgag cgcgagaacg acaaaaacac agttgtggcc ttacgtgaaa ttgctgagca
    10921 aaccgttgaa gtcgaaacac tgaaagaaga gctcgtacgt tctcaccaac gcatgatcgc
    10981 ttatgaagaa gaagatgaaa gcattgacct aatggatggt gaggaagaat ggggcacaat
    11041 cgcgagcaat catggtaaag ctggcgcttc tgggatgtat gcagaagaag acatcgctca
    11101 ggatgatgag ccttctttgg aagacatggc tggcggcgct ccggcagagg aagaatagtc
    11161 ttcgcctaat catgaatttg gaaggccgga ttttccggcc ttttttacgt acggctttta
    11221 cgatttatat tcatgctttc caatacgatc catgttagat ttaacgctta aaaaaattta
    11281 atatggccga caggtttagt gtgcaatgac agatcttcca cctcttgtgg ataaaatcaa
    11341 aagctacaat cccgacttca acccacaaat tgttgaggcc gcgattgcgt ttgccaagga
    11401 taagcacgaa ggccagaccc gatcatcggg agagccgtat tatactcatc cggtcgccgt
    11461 ggcgagtatt ttggcggaaa tgaagttgga tctgggcagt attctgacgg ctattttgca
    11521 cgatacgatt gaagataccg atgcgaccta tgacgatctt aaagataaat tcggctgtga
    11581 agtcgccgat cttgttgacg gggtgagtaa actcacccgg attgaaagca aaacagtcga
    11641 gggcaagcag gcggaaaact ttcgtaaatt gctgctggct atgtccgaag atattcgcgt
    11701 attgcttgtc aagctggctg accgtctgca taacatgcgc acaattgaag ggattaaaaa
    11761 ccctgaaaag cgccgccgca ttgcgctgga aacgcttgag atttacgtgc cgctagccga
    11821 acgcatcggc gtccataaaa tcaaaggcga gttggaagat ctcgcttttt cgattctcaa
    11881 tcctgaagcg cgtgaaagta ttgccaagcg cttggccttt ctgcgtgctg agggcaccga
    11941 tttagtcgat gacatcattg cagaacttaa aaaatctctc gaagatgtcg gtataaaggc
    12001 tgaaatttca ggccgggaaa aaaccggtta ctcgatctgg aagaaaatgc agcgtaaaaa
    12061 tgtggccttt gaacagctct ccgacatcat ggcttttcgc attgtggtgg gtagcgttga
    12121 gcaatgctat gctacgcttg gccttatcca tagccattat ccttctattc ctggacggtt
    12181 taaggattat atttccacgc ccaagcccaa cggctatcgt tccatccaca ccaccgtgat
    12241 gggtccggcg cgccaacgca ttgagattca gatccgtacc agcgaaatgc actatcaggc
    12301 cgacatggga gttgccgcac actgggccta taaacagggc ggccagccca tggttcagga
    12361 tgttaaaaaa taccgctggc tgcgggaact tttggagatt ctcgaacagg aacagcaacc
    12421 tgaagatttt tgggaaaata caaagctgga attgtttcag gaacatgtct ttgccttctc
    12481 tccaaaaggt gacttgattg agctgccttc gggcgcaaca ccgattgatt ttgcctatgc
    12541 cattcactct gatattgggg atcgctgtgt cggcgccaaa atcaatggcc gtattgcccc
    12601 gcttaatacg cgcctggcca atggggatca ggtcgatatc ataacttcga aaacgcagca
    12661 accttctccg acgtgggaac gttttgtggt tacggggaag gctcgctcgc atatccgaaa
    12721 attcgtacgc aaccggcagc gcaatgaata tgtcacgctg ggcaaagcga tgctgcaaaa
    12781 gatttttcgc caggaaggct atgagtttag cgaaaaaggc gtaagcggca ttttgaaaca
    12841 attcaaagcc gaagaaaatg atgatattta tgcgcgcata ggttccggga ctctggtggc
    12901 gcgcgatgct tttaaggcta tttttccggc gcataaaacc gcgattgcca agcgtccgtc
    12961 agctgatatg gatcaagccg taatggtccg tcctttaagc ggatctgatg cgccaatgcc
    13021 gatcaagggg cttattcccg gcatggccgt gcattttgcg cggtgttgtc atccgcttcc
    13081 cggtgaccgg attgtgggaa tcgtgacgac aggaaaaggc gtgaccatcc acactattga
    13141 ttgtgaaacg ctggaaacct ttgctgacac accagagcgc tggctggatg tttcctgggg
    13201 cgaaggaccc gatagtccgg aagcacatgt ggggcgcatt aatataacat tgcagaacga
    13261 gccgggtaca ctggccacgc tttcaacggt gatcgcttcg aatggcggca atatcacgaa
    13321 tctgaaaatc accagccgtt cggtcgattt ctggcaaatt ctggttgatg tgtatgtcaa
    13381 cgataccaaa catttaagca acatcatagc cgctctgcgc gcaacgccga ttatttccag
    13441 cgtcgaacgc gcaaagggac aatagaatat gtacgatatc ctttcccaaa tcgcatggat
    13501 tgcgtttttc agtattgccg ctcaatggtt gggctggcgg ttaaaaaccc cggcgattgt
    13561 gtttttgctt atcggcggtt ttttggccgg ccctactttg aacctcgttc aacccgaaac
    13621 cctgctcggt gatttgttaa accctgttat atccctggcc gtggggatta ttctgtttga
    13681 aggtgcgctc aatctcaatt tcaaggaaat ccgcgaggcg cgtgcctcca tccgccaggt
    13741 gattttggtc ggcgcgcccg tggcgtggct tttgacaacg ctggcaggac attacgttgc
    13801 ggggttaagc ctgcctgttg cgctgacctt tggcgcgctt ttgattgtca ccggcccgac
    13861 cgtaatcatg ccgctgctga aaaacgcgca tttaaaagaa cgcccggcct ccatcctgaa
    13921 atgggaaggg attatcaacg accctatcgg cgcggtatta gctgttttat gctatgagtt
    13981 ttttcgcctt caggcgcttg aaaatgtagc cttatccgaa ttttttacca gcactgttct
    14041 ggcgatcatc atgattacgt ttttcggcgt ggcaattgcc tattcgctgt catggttgtt
    14101 taaccatgat gtggttcccg aatatcttaa gccgaccgct ttgctctcta ccgcactgat
    14161 ttattttgta atctgtaatt cgattgagca tgatttcggc ctgattggcg taaccatttt
    14221 gggtgtggcg ctggccaata tgggcataag ctcgattgaa gagcttaaaa aatttaaaga
    14281 gaccatctcg attatgctgg tttcaggcgt gtttatattg ctgaccacca aaatcgatcc
    14341 ggccattctc ttttctattg actggcgcgg ctttgcgttt atcgcaatgt tgctctttgt
    14401 gatccgtccg ttgacattgc tggcttcaag tattggcacg aaaatgagct ggcaggaagt
    14461 gatccttacc ggctggatcg cgccgcgcgg gattgtctgc gccgcagtgg ccggggttct
    14521 tgggccatat atggtcggca tcggctatca ggacggggag caaattcttg cgctggcctt
    14581 cgctattgtg ctgttcaccg tttttgctca cggtctgagc gccaagccgg tcgcccaaaa
    14641 actgaagctt gcctatacgg gcaaggacag tttgattatc gttggagcct ctgagtgggc
    14701 ggtgcaattt gcaaagatgc ttaaaggccg tgacgtcgat gttatgatcg ccgataaaaa
    14761 ctggcacgcc ttacgtgagg ctcgtttgtc ggatattcct acatattatg gagaaattct
    14821 gtctgaagaa actgaatatc atcttgagct tgcgcgttat aatactttgt tggctgttag
    14881 taataaccca gcctataacg cgctgatttg caatatgttt attcatgaat ttggccgcga
    14941 caaggtctat caatttttgc cgcatgaaga aaacgagcat gagcgccgca aaattgccca
    15001 caccattcgc ggacgcacct ttgggcaagc cgatatggat tattgggata ttgccggcga
    15061 ttatatgcaa ggatggcgct ttcgtgccag ccgcgccggg caggaagaaa accgtattca
    15121 gtttcagcag cgcattgaag agggcaattt aaaaatcgtc ggatatatca cacaaaaagg
    15181 acggcttgat cttcgtcagc ctgagagcct cgagaaattc gaagaacagg acctgcttat
    15241 tttctttgaa caaggagatt cgtaggaaga ggctaaaatc aaaaaacaat ctatgctgga
    15301 cagcgaataa ccgattccag gggtgctgtg agtttatcgc gcccgttaag accctctagc
    15361 tcaatgatgc ataaatttgc cacaacatgc gcgccggcct tatgcagcaa tatttcagcc
    15421 gcctgcatcg tgccgccggt tgccagcaaa tcatcaacca atacaacctt ggagcctgcg
    15481 ggaatcagat ccggctggat ttctataata tcttcgccat attcaagcgc gtaaccatgc
    15541 gccaacgttt ggcccggtaa cttgccgtgc ttgcgtacca tgccaaaacc acagcccatc
    15601 tcataggcca gcggcgcggc caccaaaaac ccccgcgatt caatgcccat cagcaaatcc
    15661 ggctgataat cctccgttaa acggtgaagc cgtgcgatgg tttcacgcca tgccgatggg
    15721 ttggccagca aagtcgcaat gtcatagaaa ttaatccccg gcttgggaaa atcaggaata
    15781 gtgcgaatgt ggtcttgaat attcatgaga ttgccttcat ctttctaatg ctgaatcttc
    15841 gtgataataa gctgaaaacc ttacaacgta aatgacggcc tctataaatg tttaaacggc
    15901 gaacgccacg tacagtttta caaaatatca gagaactgtt ctggccaagt atgggctggg
    15961 ttagggcggc gcattataca aagcaccggg tggtcaggct ttctgacagc acccagaaaa
    16021 tcgctttcgg gctggctatt ggcgtggcta tttcctttac gcctatttta ggtacgcatt
    16081 ttgtacaggc cggatttctg gcctatgtct tgcgcgctaa ttttttggcg gcgctcattg
    16141 gtacttttgc gggaaaccct tggactttcc cgtttatgtg gtgggcggct atttcattgg
    16201 gctcgtttat atttcagctt ttgggcttgc ctgccgaagc ggcgctgcct gaacacgctg
    16261 atttctgggt tatctgggac ctgataaaaa gtgaacctta tcggatattc gcgccgtggt
    16321 ttgttggcgg ttatttactg ggggcactta ctgttccgct cacatacagt ttgttttatc
    16381 gtttagtcgg cagtgcacag gcggcccgta aaaaagcgcg ggaaaaccgt ttgcataagg
    16441 ttgcacggga agttaccggg cagaaaaaat aaggtacacc tctatgatca ttggcatagg
    16501 caacgatctg attgatataa accgtg
//
LOCUS       DAOSWR010000035        16496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2202_length_16496_cov_5.088437, whole genome shotgun sequence.
ACCESSION   DAOSWR010000035 DAOSWR010000000
VERSION     DAOSWR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 16496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16496
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_16496_cov_5.088437"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1072
                     /locus_tag="PK513_04165"
     CDS             80..1072
                     /locus_tag="PK513_04165"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOO81677.1"
                     /translation="MSESNLKIGVVGAGAWGTALAQMLSNGGRDCLIWAREPEIVESI
                     NTRHENTVFLPGIALSKPLRATGDLTETIQRDIVLMVTPAQHLRTTLKTIKNDLRPET
                     ILVLCSKGIELDTGLLLSEVCEQIIPEAKIAVLTGPTFASEIAAGLPAAATIGVSDPE
                     TGKTLQNALGVKGFRPYISNDVIGVQLGGALKNVIAIACGVVYGRKLGESARAALLTR
                     GVTEISRLGKAMGAQEETFMGMCGIGDLMLTASSMQSRNFSLGAALGEGKTLEEILKQ
                     RNAVTEGVHTAHAALNLAEKYDVEMPITQAVYQCLHDNLPVDQAIENMLTRPLK"
     gene            1400..1687
                     /locus_tag="PK513_04170"
     CDS             1400..1687
                     /locus_tag="PK513_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81678.1"
                     /translation="MIDDEDYKSLLFPYNLIGDDDGLSETALVPDQEVHKIGIDYIIE
                     YSFFTSQSDILYSGYYLSTDEEVVKFIGSSDYQQNIEDNLRLIFAPSLKEK"
     gene            1689..2111
                     /locus_tag="PK513_04175"
     CDS             1689..2111
                     /locus_tag="PK513_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81679.1"
                     /translation="MHCPITKINIPRFLIATIVGFAFVFGLDFLVHQNLFSNIYEQTT
                     HLWRPVDEIASFMPYMFGYQALLVIIIGFIYTRNHKGKGITEGLRFGLQIGVLLALLN
                     AAAYIWMPIPKALALSWAAVGLGTGLGLGIIFSLLYKK"
     gene            2123..2542
                     /locus_tag="PK513_04180"
     CDS             2123..2542
                     /locus_tag="PK513_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOO81680.1"
                     /translation="MAYWLLKSEPYKFSWDDQVKKGNGRWEKRVRNYQARNNLAAMKP
                     GDKGFFYHSNEGKEIVGILEVTSEAYTDPDPEKNETGKFVTVDVKPLTALKNPVTLAD
                     LKSHPVLSNMKVVKQVRLSVSPVTEEEWEIVTAMGSA"
     gene            2713..2955
                     /locus_tag="PK513_04185"
     CDS             2713..2955
                     /locus_tag="PK513_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81681.1"
                     /translation="MKNIYNFRQIKYKFYQFLLDERGSTALEYGLMLAGFSLAIAGAT
                     SLIGNELSTTIHDGLSPLLSTQIESSEGGTSGHLLR"
     gene            3077..4999
                     /gene="acs"
                     /locus_tag="PK513_04190"
     CDS             3077..4999
                     /gene="acs"
                     /locus_tag="PK513_04190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOO81682.1"
                     /translation="MADTFAPSKDILDNVGISEDQYKAMYKGSIEQPEIFWAVQAKRI
                     DWFKKPTIIKNTSFEGDVSIKWYEDGQLNACFNCIDRHLPEKENDVALIWESDEPDID
                     NKVTYGELLKEVSRLANALKQLGVKKGDRVIIYMPMVLEATYAMLACARIGAVHSVVF
                     GGFSPQSLHDRIVDCDAKIVITADEGVRGGKTIPLKNNCDEAVKNTPVEKVLVLEHRD
                     GEISWDNNRDVWWHDLVDDQPNECPCEIMHAEDPLFILYTSGSTGKPKGVLHTTGGYM
                     VYAALTHEVAFDYKPGEIYWCTADVGWVTGHSYIVYGPLANGATSLIFEGVPNYPDWS
                     RFWNVVEKHKVNIFYTAPTAIRALLREGDEPVTKTDRSSLRILGTVGEPINHEAWMWY
                     YNVVGEGRCPIIDTWWQTETGGFIITPLPCHTLKPGMAQKPFFGIKPAIVDSEGKILD
                     GETEGNFVILDSWPGQIRTVFGDHERFIKTYFSDFPGMFTTGDGAKRDKDGDYRITGR
                     VDDVINVSGHRLGTAEIEDAINEHADVAESAVVGYPHEIKGTGIYAFVTLAAGKQPSE
                     EIKNAVIQTVRAEIGPIASPDIIQWAPGLPKTRSGKIMRRILRKIATHEEDHIGDTST
                     LADPAVVEDLIKNRSK"
     gene            complement(5000..6424)
                     /locus_tag="PK513_04195"
     CDS             complement(5000..6424)
                     /locus_tag="PK513_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO81683.1"
                     /translation="MTDFSEKASSFEPQTVLLVGAGVIGRSLALAYAQNFPNFEICLY
                     DRDPQICAALQDVIQERGAENVCVLSDMNSFLQAARRAAVFDQCVPIDKMGDVQRFVR
                     GVLPKGCIVTNRGSAQVYALQQILPHVEQGRVHFGAHMLVGREASPGDELHCYPEMFA
                     GQICVIEALPENANALERDAHAKLMDINQRLGMDVKVLPAYIHDQLLGAFSHENTAAL
                     RVLVNATKGKATGLGATIMRASVGSETMWMPVYRFNQQAMVRACDIQEEALGKFKKAL
                     LLSDRALFSQLLRDAHDFRMRWPDADEPVDSLLGTMGELNGQGHDEHALVQKVFMPLL
                     FSVSRTLGFRDVVGRLGPELERYGHHFTDLLNTSAKDSTLAAKYNPDMLASLMWEHRT
                     GLLSELYHYQEQYRIFSNQLSSIGCDPEAAKALGASIRNTAEIMNGGADILPRRAGGG
                     QGFAFLDCPTSAGAQEFPLLQQLD"
     gene            complement(6506..8362)
                     /locus_tag="PK513_04200"
     CDS             complement(6506..8362)
                     /locus_tag="PK513_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO81684.1"
                     /translation="MISVEKQYLWPFFLTVPLILVSFASGMFFRGTDNILIFPHLMAM
                     ACFAAFSLWRGREQVWELPRGPLVFALLAYWFYVIISISWSTNTYNSTLFAIIFSVLP
                     FMFFALVVTPQPLRWLQVHMGALAVAVAIVSVWAGVQFFAIEPGRRVHHPMLNPNNLA
                     VVFNMALFPALALYFCARRRALMLIGFGLSALFLLAMFTTQSKGGLLSFLIVLAVFFI
                     FVRGQAGMNWRRIVAIAIVAFSLFAVVHAVSTKSFGGSFLEVVAPQDVESKTVSERKL
                     IWASTRHLIQDHFWGGTGLGTFTYFYPRYRHPKDYSDGYFAHMDPLQFWAEMGVMAPV
                     LFYLVLLLVLIRTVRATMMCAPNSLERGWIYACFCGLLALAGHAHISFHLYMPINLFL
                     AAFLLAGWFIATEAALKSSRYSLEAHHNWIKMAIYLTLLVIVFAPMVWIARATAGVHY
                     LNQVDSLMQAGQEEEARLALDQARRYSPKNYSSLYDVEAKYRLHMLNAKRQNMPPEQR
                     RKMYEEAHNYIDRASALNPGFVNLLNRKALIYFAGFPDIDPKGRDKAQTLLEEALAAN
                     PILLDVRIGLAHMYFIGGDKDKALAVMQDLYNYPVPKTFTPEQYQAVVKKFQ"
     gene            complement(8408..8740)
                     /locus_tag="PK513_04205"
     CDS             complement(8408..8740)
                     /locus_tag="PK513_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HOO81685.1"
                     /translation="MSDAAVDYEVDEIDETETQNDSEASGGGESQDVGGVAGQRLRSF
                     IERIERLEEEKAALAEDIKEVYAEAKGVGFDAKTMRKIVSLRKMDYEKRRESEELLDL
                     YKTAIGMV"
     gene            complement(8855..9523)
                     /locus_tag="PK513_04210"
     CDS             complement(8855..9523)
                     /locus_tag="PK513_04210"
                     /inference="COORDINATES: protein motif:HMM:NF022835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HOO81686.1"
                     /translation="MQRFLFLVCFCIGAMFVSQPAHAGKLLEFFFPSLRDAGPDPSQT
                     LKAPFAEDDVVAANEGGGAAHSEASMVPLHLPHRSEEIISTWVVTAVSDSLNFNGQNY
                     QDDIKKSFVHFDAGGRSEFEAFLKDNKIMDVLQSSQYSVQSYVSAPPLLLNEGAVNKR
                     YRWLYQVPVTVSYLKKGTTSYKGVEATNQHFLLQVQVGRNEEAKGDLGTFVERWSGRS
                     VKKP"
     gene            complement(9523..10119)
                     /locus_tag="PK513_04215"
     CDS             complement(9523..10119)
                     /locus_tag="PK513_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HOO81687.1"
                     /translation="MKQADDIQNKDKILEKVLPEVAFDGWHWSLVQRIARKEGYSAEG
                     LHALFPGKLKDVLAAFSDLADRKMLEVLQEVDPEDLRIRDRVSTALMARFEWLAQHKE
                     ALRQSLQFWMVPMNKPCGARLVWHSADKIWRYAGDRATDYNHYTKRGLLSGVIVSTTL
                     AFLNDETETLDNTRAFLDRRIENVMQLGKVINKVKRVS"
     gene            10178..11542
                     /locus_tag="PK513_04220"
     CDS             10178..11542
                     /locus_tag="PK513_04220"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOO81688.1"
                     /translation="MKNKADFKENTIGTKLTRYGKVSGTMAGLAAKIAGEKFLGLKIE
                     REEHAQALMTALGNLKGPLMKIGQILATIPEALPPEYANAFQELQADAPPMGWPFVRR
                     RMKTELGPDWEKRFETFEHEAAAAASLGQVHKGLAKNGQAIACKLQYPDMKSAIETDL
                     NQLKIIFGIYERYDKAIQTNQIYAELAERLLEELDYAREAKHCKLYEYMLRSEPSVHV
                     PHVIDDLSTDRLLTTSWMEGNKILTYKDAPQTQRNAIAMNMFRAWYVPLYYYATIHGD
                     PHLGNYTVREDNSINLLDFGCVRIFPQKFVGGVIDLYHALMNKDNELAVHAYETWGFT
                     DLTKEQVETLNIWAEFLYGPIMDDKVRAIGHADGAVYGRDTAEKVHARLRELGRQRGG
                     ITVPREFVFMDRAALGLGSVFIHLNAQINWYRVFNELIEDFDVDALATRQKESLTRFG
                     LLKT"
     gene            11833..>16496
                     /locus_tag="PK513_04225"
     CDS             11833..>16496
                     /locus_tag="PK513_04225"
                     /inference="COORDINATES: protein motif:HMM:NF017472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81689.1"
                     /translation="MTHIRAQQTHINPTQNPKQQRPQTTKRLNERGNVFFTLFGAVAI
                     VGVLGAGIMSTMRGPLSTMVEVNRREQAKAEARVAASLVLVNASDEAVEIICEGGTIG
                     VDEDGYTEGPEPVGGGGPTGGGTLPAVGAPQNDPWGNAYGYCAWDHGLAAADYSGCGN
                     YLAGNVDTNNIAIAVLSAGPNGVFNTTCVDHAGGYMTPNTGGGGDDIVVSMTYNQAIA
                     GSGGLWNLKATDPNIAEINKNLEVTGGGKFTDVLDLTDAAAQLQLGEKSLTLPTQATL
                     TTCNGANENLLRINTGPNPDVVEICNEDDGSPGSFAWVSVAAGANLWSQNGSDIYYGS
                     GNVGIGLNNPNDALDVVGTAEITGATILGSTLGVTGATTLSGTLAVTGTSALNNDVTI
                     TGGATGGTTDALSVRDSSASVIFTVQNDGTVGIGLSNPAEELDVSGDIQASGIYLLDG
                     TQILSNGDDTSNILLGDVAAFSSSSNYMNLGDAIHADLTTANKRVGIGFADTYDVSAF
                     NDTLEVNGTVDISDTLNVTNDTTVGGDLTVSGDDITMATNTAGHIMVADGTNFNPVAM
                     SGDVAIDGTGATTIQNDAVQEDDIDASSFGAGTGATCLKTDGSSIFTEACSGGGGGDG
                     LGGKGLPDVLANDNDAAGANAIGFGGVAIGSTTLSSGTATLELDVTGEIGATEYCDAD
                     GNNCFTTGEMNQDIAEVLGVGNNAGGATSLINLPGLAIGSTVFSTGAQTLEVDITGDV
                     GATNYCDADGNNCFTAATVSGGGGGIWENASNVVRLSSSGAHANADFVFGSPQLEDDG
                     NASHDSRIYFDKSKGAFRAGSATGNQWNYSSSGQYSVALGYDTTASGIYSTATGYGTV
                     ASNNWSTAIGATTVASGMASTAMGATTVASGMASITMGSSTTASGAIATAMGFDTVAS
                     GSTATAIGESTVASGSYSIAMGRDVIAGSGTAGDGSGDGSIAMGLRSPPAPIGTKPKI
                     TGDQSLGIFMGDQDGGVVMSSSNTLGLFGGRMILDPNVPATELSTSTGGEQDLEMDIT
                     GDLGAINYCDEDGNDCFTAADIAALSGSALPGAPDRGIQFNSGGNFAAEANFTYTSAG
                     DLIVGSYQLDDTGTGSEDNRMFFDVSKGAFRAGGVGGTQWDDGNVGTYSTAMGAATTA
                     SGYYSTATGGATSASGILSTAMGYGVIAGSGTAGDGAGDGSFAIGLTDETVTITTPSQ
                     ITGIQSMGIFMGDQDGLVMSASNTMGLFGGKMIIDPAVPATQLAASTGGEQDLELDVT
                     GDIGAVNYCDADGNNCFTPDEVGTGVFEDNSGVIRSTSGTVNYTTADFVFGSPQLADD
                     GDANHTSRMFFDKSKGAFRAGTATGARWDDANVGTYSAAFGYDVTASGNNSFATGNSV
                     VVSGYAAFGVGEGLGVTGTHAAAIGQTITSSGWASFGQGQFIYALGNLSHASGYEAGA
                     SGVLGTARGNYASALGWGSTAWGNHVTASGDSAYALGLIASATTITTEPEVTGNQSMG
                     IFMGDQDGGVDLSDANTLGLFGGRMVIDPNVPATNLSADTALEVDGTIKMAYGGEACD
                     ASR"
BASE COUNT         4680 a         4660 c         3606 g         3550 t
ORIGIN      
        1 cgtaaaggcc cgcccccgct ggccactaga tacgctcacc gcttgaccac cacgcataag
       61 ataaggcatt ctcattccca tgtcagaatc caatcttaaa ataggcgttg tcggcgcagg
      121 ggcctggggc acagcccttg cccaaatgct cagcaacggc gggcgcgatt gtctaatctg
      181 ggcgcgtgaa cccgaaatcg tggaatccat aaatacccgc cacgaaaaca ccgtcttttt
      241 acccggcatc gctctatcca aacccttgcg cgctacaggc gaccttaccg aaaccatcca
      301 acgcgatatt gtattgatgg ttacacccgc acaacatcta cgcaccacac taaagaccat
      361 taaaaacgac ctccgcccgg aaaccattct cgtcctatgc tccaaaggca tcgaactgga
      421 taccggtctg ctgctctccg aagtctgcga acaaatcatc cctgaggcaa aaatcgccgt
      481 cctcaccggc cccacatttg ccagcgaaat tgccgccgga cttcccgccg ctgcaaccat
      541 cggcgtaagc gaccccgaaa ccggaaaaac cctccaaaac gccctcggcg tcaaaggctt
      601 ccgcccttac atatccaatg acgtaatcgg cgttcagctt ggcggagcac tcaaaaatgt
      661 tatcgccatt gcctgcggcg ttgtctatgg acgaaagctc ggcgaaagcg cccgcgctgc
      721 ccttctcacg cgcggcgtga ccgaaatatc ccgtctcggt aaagccatgg gcgcacagga
      781 agaaaccttt atgggtatgt gcggtatcgg cgatctgatg ctcaccgcct cgtccatgca
      841 gtcacgaaac ttttccctcg gcgccgcatt gggtgaaggc aaaaccctgg aagaaatcct
      901 aaaacaaaga aacgccgtca ccgaaggcgt acacaccgct cacgccgccc ttaatttggc
      961 cgaaaaatac gacgtcgaaa tgcccatcac tcaggccgta taccaatgcc tgcatgataa
     1021 tctgcccgtc gatcaggcaa tcgaaaacat gctcacccgc ccactaaaat aaggatctgc
     1081 ttcaataaac atctgtaaca ccacagacaa aaggagtgat tgagatgtct gatgagaaaa
     1141 tatcaaatgt aggagacaag aaacggaaaa tattttttta acaacgctct ttttggtttt
     1201 ttgcatcgcg ctgcttattt ttacaaatac acgaagagac ctaatcagat atgttttcag
     1261 tgttccagaa tccaaagata tagtactggg acatgaagga aattctcaag gattttacac
     1321 aacagatgaa gagctaaaaa aagcaccacc ccagagcatt acgaagcctg gaaaaataga
     1381 cttcagaagg aagtgacaga tgattgacga tgaagattat aaaagtcttc tttttcctta
     1441 taaccttatt ggggatgatg acgggctaag tgaaacagca ttggttcctg atcaagaagt
     1501 tcataaaatt ggcatagatt atattattga atactcattc tttacctcgc aatcagatat
     1561 tctctattca ggctattatc tcagcacaga tgaggaggta gtaaagttta ttggctcatc
     1621 ggactaccaa caaaatattg aggacaattt gagacttatt tttgctccgt ctctaaagga
     1681 gaaataaaat gcattgcccc atcacaaaaa ttaacatccc gcgtttcctt atcgccacaa
     1741 tcgttggttt cgcctttgtt tttggactgg actttctggt ccaccaaaac ctgttttcaa
     1801 acatatatga acaaaccacg cacctttgga gaccggttga tgaaatagca agcttcatgc
     1861 cctatatgtt tggataccag gccctgctcg tcatcatcat cggctttatc tatactcgta
     1921 atcacaaagg taaaggcatc accgaaggcc tgcgctttgg cctacaaatc ggtgtcttgc
     1981 tagcgctgct caacgccgcc gcctacatct ggatgcccat ccccaaagca ctggctctaa
     2041 gctgggcggc tgtcggcctg ggaacgggtc tcggcctcgg tatcatcttc tcactgcttt
     2101 ataaaaaata ggaaacgcgc acatggccta ttggttgtta aaatccgaac cttacaaatt
     2161 ctcctgggac gatcaggtta aaaaaggcaa tggccgctgg gaaaagcgcg tccgcaatta
     2221 tcaggcgcgc aataacctcg ccgccatgaa gccgggcgac aaagggtttt tctaccactc
     2281 caatgaaggc aaggaaatcg tcggcattct ggaggtcacc agcgaagcct acactgaccc
     2341 tgatcctgag aaaaacgaaa ccggaaaatt tgtcaccgtt gatgtcaaac cgcttacagc
     2401 gcttaaaaac ccggtcacgc tggcggacct aaaatcccat cccgttctgt caaacatgaa
     2461 agtcgttaaa caggtccgcc tgtccgtttc tccagtcaca gaggaagagt gggaaattgt
     2521 caccgccatg ggcagtgcgt aagcgaatat acgcaacatc gctctagcga catcttcgtt
     2581 atggccgtct ggagcatgta ctatactatc attcgcgtta gcacataccc taaacacaaa
     2641 aaattaaaac aataacaata aattatgaaa ttaagttggc aaaaaacatt ttcatcatgc
     2701 tatactttaa ttatgaaaaa tatatacaat tttagacaaa taaaatacaa gttttaccaa
     2761 ttcctgttgg acgaacgcgg atcgaccgcg ctagaatacg gcctgatgct agccggtttc
     2821 agccttgcta tcgcaggcgc aacaagcctc ataggtaatg aactctccac aaccatccac
     2881 gatggcctct ccccgctact aagcacacaa attgaaagca gcgaaggcgg aacatcagga
     2941 catctcctcc gttaagataa attacgtttg ccgaccctta cgtaagaaaa caagccacgc
     3001 gcgaattgtt tattgtttta gctctctctt tttaatggca tagtaaacat ctaatcataa
     3061 tttaaggagt ttacgcatgg ccgacacttt cgccccctcc aaagacattc tggacaacgt
     3121 aggcatatcg gaagaccagt acaaagccat gtacaaaggt tcaatagaac agcctgaaat
     3181 tttctgggcc gtgcaagcca aacgcatcga ctggttcaaa aaacccacca tcatcaaaaa
     3241 cacatccttt gaaggtgacg tttccatcaa atggtatgaa gacggccagc tcaacgcctg
     3301 cttcaactgt atcgaccgcc acctgcccga aaaagaaaat gacgtagccc tgatctggga
     3361 aagcgatgaa ccggatatcg ataacaaagt tacctacggc gaattactca aagaggtctc
     3421 ccgcctggct aacgccctaa aacagcttgg cgtaaaaaaa ggtgaccgtg tcattattta
     3481 tatgcccatg gttctggaag ccacctatgc catgctggcc tgcgcaagaa taggcgccgt
     3541 gcactccgtt gtctttggcg ggttttcccc acaatctctg catgatcgaa tcgtcgattg
     3601 cgatgcaaaa attgtcatca ccgccgatga aggcgtgcgc ggtggcaaaa ctattcctct
     3661 caagaacaat tgcgacgaag cagtcaaaaa cacaccagtc gaaaaagttc tcgtccttga
     3721 acaccgcgac ggcgaaatca gttgggataa caaccgcgat gtctggtggc acgaccttgt
     3781 cgatgaccaa cccaatgaat gcccgtgcga aatcatgcat gccgaagacc cgctatttat
     3841 actctatacc tccggatcga ccggaaaacc caaaggcgtc ctgcacacca caggcgggta
     3901 tatggtatat gcagccttaa cccacgaagt cgcttttgat tataaacccg gagaaattta
     3961 ctggtgcacc gccgatgtcg gctgggttac aggccactct tacatcgtct acggcccgct
     4021 tgccaacggt gcaacaagct tgatttttga aggcgtacca aattaccccg actggtcacg
     4081 cttctggaac gtagtggaaa aacataaagt caatatcttc tacaccgccc ccaccgccat
     4141 ccgcgccctg ctccgtgaag gcgacgagcc tgtgacgaaa acagaccgca gctcactgcg
     4201 cattcttggc accgtcggcg aacccatcaa tcacgaagcc tggatgtggt attataacgt
     4261 cgttggtgaa ggacgctgcc ctatcataga cacatggtgg cagacggaaa ccggcggctt
     4321 cataatcacc cccttgccct gccacaccct caaacccggc atggcgcaaa aaccgttttt
     4381 cggcataaaa cccgctattg tcgatagcga aggcaaaatt ctcgacggag aaaccgaagg
     4441 caattttgta attcttgatt cctggcccgg acaaatacgt accgtattcg gcgaccatga
     4501 acgctttatt aaaacttatt tttctgactt ccccggcatg ttcacaacag gcgacggcgc
     4561 caagcgtgac aaggatggtg attaccgcat cacaggccgc gttgacgatg tcattaacgt
     4621 ttccggccac cgccttggca ccgctgaaat agaagatgcc atcaacgaac atgcagacgt
     4681 cgccgaaagc gccgtcgtcg gatacccaca cgaaattaaa ggcaccggca tctacgcatt
     4741 cgtaacccta gcagcaggaa aacaacccag cgaagagatc aaaaacgccg tcatccaaac
     4801 agtacgcgct gaaatcggtc cgattgcctc acccgacata atccagtggg cccccggcct
     4861 gcccaaaacc cgctccggca aaatcatgcg tcgtatcctg cgcaaaatcg ccacgcatga
     4921 agaagatcat atcggcgaca catcaacact ggccgacccg gctgtcgttg aagacctcat
     4981 caaaaaccgc tcgaaatagc taatcaagct gctgcaacaa aggaaattcc tgtgcaccag
     5041 cggaggttgg acaatcaaga aaagcaaacc cctgcccgcc gcccgctcgg cgcggtaaaa
     5101 tatcagcccc tccattcata atctcggctg tattacgaat agaagccccc aaagctttgg
     5161 cagcctcagg atcgcatccg atagatgaaa gctgattgga aaatatccga tattgttcct
     5221 gataatgata gagttcgctc agaagtccag tcctgtgctc ccacatcaat gacgccaaca
     5281 tgtccggatt atactttgcc gccaaagttg aatccttggc tgatgtgttc aaaagatcgg
     5341 taaaatgatg accgtagcgc tccaactcag gccccaaacg ccccaccaca tcacgaaaac
     5401 ccagcgttcg cgacacacta aacaacaatg gcataaaaac tttctgaacc agcgcatgtt
     5461 catcatgccc ctgcccattc aattccccca tcgtgcccag caacgaatcc acaggctcat
     5521 ccgcatccgg ccacctcatc cgaaagtcat gcgcatcgcg caacaactga gaaaacagag
     5581 cacgatcaga taataacaac gccttcttaa atttcccaag cgcttcctcc tgtatatcac
     5641 acgcccgaac catagcctgt tggttaaatc gataaaccgg catccacatc gtctcagaac
     5701 caactgacgc gcgcataatc gtcgcaccca gccccgtggc cttaccctta gtcgcattaa
     5761 ccaaaacccg caaagccgca gtattttcat gcgaaaaagc tcccaaaagc tgatcatgaa
     5821 tatatgcagg caaaacttta acatccatgc ccaaacgctg attaatatcc atcagcttcg
     5881 catgcgcatc acgctctaac gcattcgcat tttccggtaa agcctcaatc acacagattt
     5941 gccccgcaaa catttcggga tagcagtgca gctcatcccc cggagaagcc tcacgcccaa
     6001 ccagcatatg cgcaccaaaa tgcacgcgcc cctgttcaac atgcggtaaa atttgctgca
     6061 aagcatacac ctgcgcggaa ccacggttgg tcacaatgca ccctttcggt aaaaccccgc
     6121 gcacaaaccg ttgaacgtcg cccatcttgt caatcggcac acattgatca aagactgccg
     6181 cccgccgcgc cgcctgtaaa aacgaattca tatccgaaag cacacataca ttctcagcgc
     6241 ctcgctcttg tataacatcc tgcaatgccg cgcatatttg cggatcacgg tcatacaaac
     6301 aaatctcgaa atttggaaaa ttctgcgcat acgctagcgc cagcgaacga ccaatcactc
     6361 cggctccaac cagcaaaacc gtctgcggct caaaagaaga agctttttca gaaaaatctg
     6421 tcatcacaaa aaatcaaccc aactctttta cacaaataag caagagccag acactataag
     6481 tattttcata aaaaatgaaa ggggtttatt gaaatttctt cacaaccgct tggtactgct
     6541 ctggagtaaa agttttcgga accggataat tataaagatc ctgcatcacc gccaatgctt
     6601 tatccttgtc ccctccgata aaatacatat gtgcaagccc aatgcgcaca tccaataaaa
     6661 tcggattcgc tgccaaggct tcttccagca aggtctgtgc tttatcacgc cccttcgggt
     6721 cgatatccgg aaaaccggca aaataaatca aagccttacg attgagcaaa ttcacaaacc
     6781 ctggattaag cgcactggcc cggtcaatat aattatgtgc ttcctcatac atttttcggc
     6841 gctgttcagg aggcatgttt tgccgcttcg cattaagcat atgcaaccga tacttcgcct
     6901 caacatcata caatgaggaa taatttttag gactgtagcg ccgtgcctga tcaagcgcca
     6961 agcgcgcttc ttcttcctga ccggcctgca tcagggaatc aacttgattt aaatagtgca
     7021 cacccgccgt tgcccgtgct atccatacca ttggagcaaa aacaataaca agcaaagtca
     7081 aatagatagc catttttatc caattatgat gcgcctctag actataacga gaactcttca
     7141 acgcagcttc agtcgcaata aaccaccccg cgagcaaaaa cgccgccaaa aaaagattga
     7201 tcggcatgta gagatgaaag ctgatatgcg catgcccggc caaagccaac agcccgcaaa
     7261 aacacgcata tatccaaccg cgttccagcg aattcggagc acacatcatc gtcgctcgaa
     7321 cagtacgaat aagaaccaaa agcaaaacca gataaaacaa aaccggggcc atcaccccca
     7381 tctccgccca aaactgtaac gggtccatat gtgcgaaata cccgtcgcta taatcttttg
     7441 gatgtctgta ccgcggataa aaataagtaa acgtccccaa accggtcccg ccccagaaat
     7501 gatcctgaat caaatgcctg gttgaagccc aaataagctt gcgctcagac actgttttag
     7561 actcaacatc ctgcggcgcg acaacctcaa gaaacgatcc gccaaaagac ttcgtactta
     7621 ccgcatgtac aacagcaaaa agactgaaag ccacaatcgc tattgcaaca atgcgccgcc
     7681 aattcatccc tgcctgcccg cgcacaaaga taaaaaacac cgccaataca atcaaaaacg
     7741 aaagcaaccc gcctttactc tgcgtcgtaa acatcgccag caaaaacaac gcgcttaaac
     7801 caaatccaat cagcatcaaa gcccgtctgc gcgcgcaaaa atacaacgcc aacgccggaa
     7861 acagcgccat attaaacacc acagccagat tgttaggatt aagcatcgga tgatgcacac
     7921 gcctgcccgg ctcaattgca aaaaactgca cccccgccca aacagaaaca atcgctaccg
     7981 ccaccgccaa cgcacccata tgcacctgca accaacgcaa tggctgcggt gtcaccacca
     8041 atgcaaaaaa cataaacggc aacacactga aaataattgc aaacaaagta ctgttatagg
     8101 tattcgtaga ccaactgata ctgatgatca cgtagaacca atacgccagc aaagcaaaaa
     8161 ccagcggccc acgcggcaac tcccaaacct gttcacgccc gcgccacaat gaaaaagcag
     8221 cgaaacaagc catcgccatc aaatgcggaa aaataagaat gttatctgta ccgcgaaaaa
     8281 acatcccgct tgcaaaacta accaaaataa gcggtacagt aagaaaaaag ggccacagat
     8341 attgtttctc gaccgaaatc ataccctaca gccctctatc aaacaaatta agtaaaggcc
     8401 ctagcctcta aaccatacca atcgcggttt tataaagatc taaaagctct tcagattcac
     8461 ggcgcttctc ataatccatc ttgcgcaaag acacaatctt gcgcatcgtt ttggcatcaa
     8521 acccaacgcc tttagcctcg gcataaactt cctttatgtc ttccgcaagc gctgcttttt
     8581 cctcctcaag acgctcaatc cgctcaatga agctacgcaa ccgctgtccg gccacaccgc
     8641 caacatcttg tgattcacca ccaccggaag cctcactatc attttgtgtt tcagtttcat
     8701 caatttcatc aacttcataa tcgacagcgg catcggacat aaaaactctc cttaaaagat
     8761 ttatattgat tcggatggcc caattgtaac gcccgagacc aaactgtgaa gcctcaattc
     8821 ataaaaacca aacgtctttt gataaacgat aattttaagg cttcttaacc gacctaccgc
     8881 tccagcgttc aacaaacgta ccaagatcac ccttcgcctc ttcgttccgg ccaacttgca
     8941 cctgcaacaa aaaatgctga ttagtggctt ccaccccttt ataactggtt gtaccttttt
     9001 tcaaataact aactgtcacc ggcacctggt aaagccaacg ataacgttta ttaaccgcac
     9061 cttcattcag caaaaggggc ggtgcactca catagctctg tacgctatac tgtgaagatt
     9121 gcaatacatc cataatctta ttgtctttta aaaacgcctc aaactcacta cgccctccgg
     9181 catcaaaatg aacaaacgat ttctttatat cgtcttgata gttctgaccg ttaaagttca
     9241 aagaatcaga cacagcagtc acgacccaag tagaaataat ttcttccgag cgatgaggca
     9301 aatgaagagg aaccatactg gcctcactat gcgccgcacc ccctccctca ttcgcggcaa
     9361 ccacatcatc ctcggcgaaa ggcgctttca gagtctgaga tgggtccggc cctgcatcac
     9421 gcaaagacgg aaagaaaaat tcaagcagct taccggcatg cgctggctga gaaacaaaca
     9481 tagccccaat acaaaaacac acaagaaaaa gaaaacgctg cattacgaca cccttttaac
     9541 cttgttaata accttaccca attgcatcac attttcgata cgtctgtcta ggaaagctct
     9601 ggtgttgtcc agcgtttctg tttcgtcatt taaaaacgcc agcgtcgtcg aaacgataac
     9661 ccccgaaagc aacccccgct tggtatagtg attgtaatct gtagccctat ccccggcata
     9721 cctccaaatc ttatctgcac tgtgccacac gagcctggct ccacaaggct tattcatcgg
     9781 aaccatccaa aattgtaaac tctgacgcaa agcctcctta tgctgtgcaa gccactcaaa
     9841 acgcgccatc aaggccgtag aaacacggtc ccgaatgcgc aaatcttcag ggtcaacctc
     9901 ttgcaaaacc tccagcatct ttcgatccgc caaatcggaa aatgccgcca gcacatcttt
     9961 aagcttaccg ggaaaaagag catgcaaccc ttcggcgcta taaccctctt ttcgcgcgat
    10021 acgctgaacc aaagaccaat gccaaccgtc gaaggcaacc tctggaagca ctttttctaa
    10081 aatcttatct ttgttttgaa tatcatcagc ctgtttcata tctggataat gtcattttta
    10141 cctagacatt ccaaccaaaa ccgccatctt atctcccatg aaaaataaag cagacttcaa
    10201 agaaaacaca attggcacga agctaacgcg ctatggcaaa gtttccggca ccatggcagg
    10261 actggctgcc aaaatcgccg gcgaaaaatt cctcggactg aaaatcgagc gcgaagaaca
    10321 tgctcaggcg ctcatgacgg cgctgggaaa tctaaaaggc ccgctgatga aaatcggaca
    10381 gattctggcg actattccag aagcccttcc tcccgaatac gccaatgcct ttcaagaact
    10441 tcaagctgat gccccgccaa tgggctggcc attcgtccgc cgccgcatga agaccgaact
    10501 cggaccggac tgggaaaaac gttttgaaac gttcgaacac gaagccgccg ccgcagcctc
    10561 cttgggacaa gttcataaag gattagctaa aaacggccaa gctattgctt gtaaactaca
    10621 atatcctgac atgaaaagtg caattgagac tgatttaaat cagctcaaaa tcattttcgg
    10681 tatctatgaa cgctacgata aagccattca gacaaaccag atctacgccg aattagccga
    10741 acgcctttta gaagaactcg actacgcccg cgaagccaag cactgcaaac tttacgaata
    10801 tatgcttaga agtgaaccga gcgtccacgt cccccacgtc atcgatgacc tatctaccga
    10861 ccgcctgctc accaccagtt ggatggaagg aaacaaaatc ctaacctata aagacgcccc
    10921 gcaaacgcaa cgcaatgcca tcgccatgaa tatgttccgc gcgtggtatg tcccgcttta
    10981 ttattacgcc accatccacg gcgacccgca tttaggaaac tacaccgtgc gtgaagataa
    11041 cagcatcaat ttgcttgatt tcggctgtgt ccgtatcttc ccacaaaaat tcgtcggcgg
    11101 cgtcatcgac ctttaccacg cattaatgaa caaagacaat gagttagccg tccacgctta
    11161 cgaaacctgg ggctttacag acctgaccaa agaacaggtc gaaaccctga atatctgggc
    11221 cgaattcctc tatggcccga tcatggatga caaagtccgc gccatcggcc acgccgacgg
    11281 cgccgtttac gggcgcgata ccgccgaaaa agtccatgcc cgtctccgcg aactcggccg
    11341 acagcgcggc ggcataaccg tcccgcgcga attcgttttt atggacagag ctgcactggg
    11401 cctcggctct gtctttattc acctcaatgc gcaaatcaac tggtatcggg tctttaacga
    11461 acttatagaa gattttgacg ttgacgctct agccacccgt caaaaagaaa gcttgacccg
    11521 attcggtctc ctcaaaacgt aattccgcca aaaatcattc agaatcacca accgattcgc
    11581 aaatattaag acattataaa aactataaaa aaccataaaa tacgtacacc acctctttgt
    11641 acgcatcatt atggaataat ttgtgcatat ccacaggtct caacctcatt tttttagaaa
    11701 cgataaggat ttagtaaggt ttcttattca tacttgaagg tgagattctt aaattgttgt
    11761 tgttttacaa aatctggggc ttgggggctt acgcaggtgg acgcgaataa atttttcggc
    11821 atattttcac gcatgacgca catccgcgca cagcaaacac acataaaccc aacccaaaac
    11881 ccaaaacaac aacgccccca aacaacaaaa cgcttaaatg aacgtggcaa cgtgttcttc
    11941 accctctttg gcgctgtcgc catcgtcggc gttcttggcg ccggcatcat gtccaccatg
    12001 cgcggccccc tctctaccat ggtcgaagtc aatcgccgcg aacaggccaa agccgaagcc
    12061 cgcgtcgccg cctctctcgt ccttgtcaac gcaagcgatg aagctgttga aatcatctgc
    12121 gaaggcggaa cgattggcgt tgacgaagac ggctatacag aaggccctga accggtcggc
    12181 ggcggcggcc caactggtgg cggaacgcta cctgctgtcg gcgcccctca aaacgaccct
    12241 tggggcaacg cttatggcta ttgcgcatgg gaccacggtc ttgccgccgc tgattatagc
    12301 ggttgcggca actatctcgc gggaaacgtt gacaccaata atatagccat tgccgtttta
    12361 tccgccggtc ctaacggcgt tttcaatacg acttgtgttg atcatgcggg cggctatatg
    12421 actcccaata ccggcggcgg cggcgacgac atcgttgtat ccatgacata taatcaggcc
    12481 attgcaggat cgggagggct gtggaatcta aaagccaccg atcctaacat cgcagaaatc
    12541 aacaaaaacc ttgaagtcac cggcggcggt aagtttactg acgtcctcga cctcacagat
    12601 gccgctgctc agcttcagct tggtgaaaaa tcattaacct tgccaacgca agctacactg
    12661 actacctgta atggcgccaa cgaaaacctg ctgcgtatta atacaggacc aaacccggat
    12721 gttgttgaaa tctgtaacga agatgatggt tcccccggca gcttcgcctg ggtatcagtg
    12781 gcagcaggtg caaatctctg gagccaaaac ggctctgaca tctattatgg ttcaggaaat
    12841 gtcggcatcg gcctgaacaa ccccaacgat gctctcgacg tcgttggcac agccgagatc
    12901 acgggcgcaa cgattttggg aagcacactt ggcgtaaccg gagccaccac actctcagga
    12961 acgctcgcgg ttaccggaac atcggcgctt aacaatgatg tcaccatcac cggcggcgcc
    13021 acaggcggca cgaccgatgc cctcagcgta cgcgatagct ccgcctccgt tattttcacg
    13081 gtacaaaatg atggcactgt tggtatcggc ttaagcaacc cagccgaaga actcgatgtt
    13141 tccggcgaca ttcaggcttc aggcatttac ctgctcgatg gcacacaaat actcagcaat
    13201 ggcgatgaca cctccaatat cctgcttggc gatgtcgccg cattcagctc aagctcaaat
    13261 tacatgaatc tcggcgatgc catccacgca gacctgacca cagcaaacaa acgcgtcggc
    13321 atcggatttg ctgacacata tgatgtcagt gcctttaacg acacactcga agtcaacggc
    13381 accgtcgaca tctctgatac gctcaatgta accaacgaca caactgttgg cggagatctc
    13441 acagtctccg gcgatgatat taccatggcc accaacaccg ctggccatat catggtcgcc
    13501 gacggcacca acttcaaccc tgttgcgatg agcggcgacg tggcaattga cggcacaggc
    13561 gcaaccacca ttcaaaatga cgcagttcag gaagacgata tagatgccag cagttttggc
    13621 gcaggcacag gcgcaacctg tctaaaaact gatggctcaa gcatcttcac agaagcttgt
    13681 tccggcggcg gtggcggtga tggtctcggc ggcaaaggcc tgccggatgt tctcgctaat
    13741 gataacgatg cagctggcgc aaacgcaatc ggttttggcg gtgtagcgat tggttctaca
    13801 acgctaagct ccggcacagc cactctcgaa ctcgacgtca caggagaaat cggcgccacc
    13861 gaatattgtg atgcagacgg caataactgc ttcaccaccg gcgagatgaa tcaggacatc
    13921 gccgaagttc ttggtgtcgg caacaatgca ggcggtgcca ccagcctgat caacctgccg
    13981 ggcctcgcca tcggctcaac cgtcttcagc accggcgcac aaacactaga agttgatatc
    14041 accggagatg tcggcgctac caattactgc gacgcggacg gaaataactg tttcacagca
    14101 gcaacagttt ctggcggcgg cggcggaatc tgggaaaatg cctccaatgt tgtgcgcctg
    14161 tcaagctcag gagctcacgc aaatgcggat tttgtgtttg gtagtccgca actcgaagat
    14221 gacgggaatg ccagccacga ttcccgcata tatttcgata aatccaaagg cgccttccgt
    14281 gcgggatctg ctactggaaa ccaatggaat tactccagct ccggccaata tagtgtagcg
    14341 ctcggttacg atacaacagc tagcggaatt tacagcacag ccacagggta cggaacagta
    14401 gccagcaata actggagcac cgcgatcggc gccaccacag tggccagtgg catggcaagt
    14461 acggcgatgg gtgccaccac agtggccagt ggcatggcaa gtatcacgat gggaagcagc
    14521 acaacagcca gcggagctat cgctacagcc atgggctttg acacagtcgc cagcggtagt
    14581 acggccacgg caatcggtga gagcacggta gcaagcggct cttacagtat cgcgatggga
    14641 cgagatgtta ttgccggatc tggcacagcc ggtgatggct caggtgacgg ctccattgcg
    14701 atggggttaa gaagcccgcc agctccaatc ggaacaaaac caaaaatcac aggagatcaa
    14761 tccctcggca tcttcatggg cgatcaggat ggcggtgttg taatgagctc ttccaacacg
    14821 ctcggcctgt ttggcggacg catgattctg gatcctaatg ttcccgccac agagctttca
    14881 acctccaccg gcggggaaca ggacctggaa atggacatca caggagatct gggcgctatc
    14941 aattattgcg atgaagacgg gaatgactgt tttacagccg ccgatattgc cgccctgtcc
    15001 ggcagcgccc tgcccggtgc gcccgaccgc ggcatccagt tcaactccgg cggcaacttc
    15061 gcagccgagg ccaacttcac ctatacgagt gcaggtgacc tcattgtcgg gtcttatcaa
    15121 ctcgatgata ccggcacggg atcagaagac aaccgcatgt tctttgatgt ctccaaaggc
    15181 gccttccgtg ccggaggtgt tggaggcacg caatgggatg atggaaatgt cggaacttac
    15241 agtaccgcga tgggtgctgc taccaccgct agcggctatt acagtacagc gacggggggt
    15301 gctacatcag caagcggtat cctcagtacc gctatgggat atggcgtaat agcaggaagc
    15361 ggcactgccg gagatggtgc aggcgatggt tccttcgcca ttggtttgac cgatgaaacc
    15421 gtcaccatca ccacaccgtc ccaaatcaca ggcatccagt ccatggggat cttcatgggc
    15481 gatcaggacg gactggtcat gagcgcatcc aataccatgg ggctctttgg cggcaaaatg
    15541 attatagatc ctgccgttcc cgcaacacag cttgccgcct ccaccggcgg agagcaggat
    15601 ctggagcttg atgtgacggg cgatatcggc gcggtgaatt actgcgatgc cgatggaaat
    15661 aactgtttta ccccagacga agtcggcacc ggagtttttg aagataattc cggcgtaatc
    15721 cgctccacca gtggcacggt taactatacc accgcagatt ttgttttcgg ctccccgcaa
    15781 cttgctgatg atggcgatgc caaccacact tcccgcatgt tcttcgataa atccaaaggg
    15841 gccttccggg cgggaactgc tacaggcgcg cgatgggatg atgccaatgt cgggacttac
    15901 agcgcagcct ttggctatga tgtaacagcc agcggtaaca atagttttgc aaccggaaat
    15961 tcggtagtag tttccggcta cgcagccttt ggtgttggag aagggctggg tgttacggga
    16021 acacacgccg cggcgatcgg acagactata acgtcaagcg gctgggcaag ctttggacaa
    16081 ggacaattta tctatgcgct cggaaatctc tctcatgcat ctggctatga ggcaggagca
    16141 agcggtgtcc tcggcacggc cagaggaaat tatgcctcgg ccttaggctg gggcagcacg
    16201 gcctggggta accatgtcac cgccagtggt gatagcgcct acgcactggg tctgattgcc
    16261 tccgcaacga caattacaac agaaccagaa gtcaccggca atcagtccat ggggatattc
    16321 atgggcgatc aggatggcgg tgttgatcta tctgatgcta acacactcgg cctcttcggc
    16381 gggcgaatgg tcatagaccc gaatgtcccg gctacaaatc tttccgccga cacagcgctg
    16441 gaagtcgacg gcacgattaa aatggcctat ggcggcgaag cctgtgatgc gtcccg
//
LOCUS       DAOSWR010000036        15903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2315_length_15903_cov_5.713276, whole genome shotgun sequence.
ACCESSION   DAOSWR010000036 DAOSWR010000000
VERSION     DAOSWR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15903
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_15903_cov_5.713276"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..272)
                     /locus_tag="PK513_04230"
     tRNA            complement(199..272)
                     /locus_tag="PK513_04230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Gly,seq:tcc)
     gene            complement(448..522)
                     /locus_tag="PK513_04235"
     tRNA            complement(448..522)
                     /locus_tag="PK513_04235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Gly,seq:ccc)
     gene            complement(625..1332)
                     /locus_tag="PK513_04240"
     CDS             complement(625..1332)
                     /locus_tag="PK513_04240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO81690.1"
                     /translation="MSAPKAYLTIDDSPSPDTEKLVDFLTARHIPALLFVRGALLEQN
                     PSAIEYAITKGLRIGNHSYAHKPAGDMQPQDWADDLEKCDHLIEAAYARCGISRPAKY
                     YRFPYIDRGDGCKIEQIDSAGIVENNKTSILQQYLHDQGFSQPFADMPSSYPSNTVDC
                     LYTFTARDWMLNDTHRGRQPIKTREDLIARAEQDEGLKVADHPHIFLLHDQPGIFEDV
                     CALIDYFCENNFEFLDF"
     gene            complement(1329..2258)
                     /locus_tag="PK513_04245"
     CDS             complement(1329..2258)
                     /locus_tag="PK513_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOO81691.1"
                     /translation="MSILADIGGTYARFAVSSPKGPEKIAKYEAADFENLQEALAHYC
                     KEHELDPSGALRVAAAGSPENGLWAFVNKNTWVIDPKELEGEGWQVERIVNDFEAAAW
                     SLPVLEECDLKALRAGAPASPSKCILGPGTGLGLAYVHQGDKPIVQPTHGGHLPVACL
                     TDEQALVIQAVQRFKGNETIPVYENVVSGPGLYNLYRALCLIGGSSVQVQSPEEMMRQ
                     ADDLKVSTALRLFHEFLGLFAASSVIAGHAYGGLYLTGGVLGRLIEAGQFDQEHFKHW
                     FCLKGIAPVTRDLRAMPIYHITYPYPALKGLNA"
     gene            complement(2309..3052)
                     /locus_tag="PK513_04250"
     CDS             complement(2309..3052)
                     /locus_tag="PK513_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOO81692.1"
                     /translation="MLKDHHHSPEEIKARLSAGPGVFYLKEWVYGGIDGVVTTFAVVA
                     GVVGAGLSPAIVMVLGLANLLADGFSMAASAYSAMKAEEDNYKRLHAREVRHVAQHHD
                     GEIEETRQIFASKGFKGEELEQMVSTISRDKDIWIEFMLQEEYGIARPIHSAMKAGVQ
                     TFWAFFICGAMPLIPFLLPGAILPTSYQPVLALILSAITFFAIGSLKSLWSLKSLPRE
                     GFETLFIGLTAAAMAYGVGYALQTWVQGG"
     gene            3101..4174
                     /locus_tag="PK513_04255"
     CDS             3101..4174
                     /locus_tag="PK513_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOO81693.1"
                     /translation="MSIQNRYDRQRILSEIGAAGQTKISTSSVLCVGAGGLGCPALLY
                     LAAAGVGLIGIVDFDVVEETNLQRQVLFDTSQIDQNKAQAAKVRLNALNPEIDIQAYA
                     EELSDKNALALFEKYDIIIDGTDNFPAKFLISDAAVKAGKPFVYGSILGFDGQLAVFN
                     YKGGPCYRCLFPEAPKGHIPNCAQAGVIGAVAGMIGTAQAMEAIKVIVDHESFKPLAG
                     KLWTIDMHSMENRLLTLPKTSDCPVCSKTKEEIALNYNSPTCGLIPEITPQQAKDKQT
                     ALLIDVREIDEWNAGHIEGAQHVALSVLVQGHVPELPPDCEIVLYCQKGMRSLQAAQI
                     LKGHGCTNIMNMTGGYEAWLKSS"
     gene            4264..5448
                     /gene="ubiM"
                     /locus_tag="PK513_04260"
     CDS             4264..5448
                     /gene="ubiM"
                     /locus_tag="PK513_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HOO81694.1"
                     /translation="MAMKHDIIIIGAGPAGLSFAREMAETGLNIVLLEKQPESVLANP
                     PYDGREIALTHLSRKIMGDLGMWDSIPDEKISLIKTAKVLNGGSPYALSFDHEEAGEE
                     TLGFMISNHQIRKAAYESLAGYKNIQILSEKEVSTLGTDQEKGWVELSSRERLEAPLI
                     VAADSRFSATRRMMGISTSMLDFGRTCIVCTMSAELPHHETAYECFHFDRTLAVLPLN
                     NNQVSIVITLGSEESAQVLAMGKEDFAADIQHRFEGRLGTMKLTSELFPYPLVATFAK
                     TFCANRFVVIGDAAVGMHPVTAHGFNLGLRSAHTLAKEIKDALQTGSDYAAQTVLQRY
                     SQKHTHASALLFHGTNALVKLYTKDTPAAKFARRTLLRLGNRLKPAKRLIMNQLTENK
                     AA"
     gene            complement(5452..6177)
                     /locus_tag="PK513_04265"
     CDS             complement(5452..6177)
                     /locus_tag="PK513_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO81695.1"
                     /translation="MSFEWKKNVAHRFDDCANEYDRHSAIQEKIAQTLAADLPDIENP
                     LVLEIGCGTGALTQRLLKCYPGGRFHITDISAHMVDRARSRIGEQKNVRWSVMDGESP
                     AKDQHYDIIVSNMAFQWFGNPHGTFETLLGLLESGGVIFYTVPAPESFPEWRASLKEF
                     SLDNGLLNFESWPGVFREDEIVEHHQSTLHFLRNLKKGGMNTPRIGYKTLSRKDLLKT
                     CRYADESFHGQVTWHILYGRIQG"
     gene            complement(6167..6787)
                     /locus_tag="PK513_04270"
     CDS             complement(6167..6787)
                     /locus_tag="PK513_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO81696.1"
                     /translation="MSADIIFVHGWGTGSAFWDRVRARLPEMQTVAIDLEFIGGGQSS
                     CTAITRESSIYITHSLGTMWALKNHHRSVKGLIAINGFSCFQHFADERTLRAMKTRLQ
                     RTPEKQMQNFWKTSGLPPSDDLNVQRLKEGLAWLSSWDVKEELKDLTCPILSLAGAED
                     PILPLALMKEEWAGYDMPVCEGACHALPLSHPDWCAEHIKDFIREL"
     gene            6999..8978
                     /locus_tag="PK513_04275"
     CDS             6999..8978
                     /locus_tag="PK513_04275"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOO81697.1"
                     /translation="MTIIAALKPEISDEITSLYGGTDVDCSAAVSEYISESDWRVKAN
                     ANTGYSNAGLINNVAGKVIANFWLDEIYAPEEGGAHRKGDIHIHDLDCLTGYCAGWSL
                     RALLNEGFNGVDGRVSSRPPRHFREALGQMSNFLGILQSEWAGAQAFSSFDTYLAPYV
                     FRDNLSFSEIKKAIRQFVYNLNVPARWGQSPFTNITLDITVPEDLADQIPTNNNLHLF
                     KGLDDDDLLRRAQQRDMGLRSLEDTSFKHFLPEMELIVIAYYEVMTEGDSSGKPFTFP
                     IPTVNITEDFDWDSKVAQAIFDNTAKVGSSYFQNFVGSQWTRDKNGERVRNPEAYSPG
                     AVRSMCCRLQLDLRELLKRGNGLFGSAEMTGSLGVVTLNMARLGYRFKGDMAGLYKEL
                     DRLMDISRSTLEKKRAYVQKMYDRGLYPYTARYLPFFRNHFSTIGVNGMNEMIRNFTE
                     DQHDLTDERGIELSMEILDHMRARMVEYQEATGNLYNLEATPAEGTTYRFAKEDAKHY
                     PGILQAGAGENIYYTNSSQIPAGHTDDPFEALELQNKLQCKYTGGTVLHLYMGEKLSS
                     AEACKRFVKKVIENYQLPYITVTPVFSTCETHGYLNGEQPECPHCGEETNVWTRVMGY
                     FRPVASFNTGKKGEHKERKHFLERRIESADLFEKA"
     gene            8981..9730
                     /locus_tag="PK513_04280"
     CDS             8981..9730
                     /locus_tag="PK513_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOO81698.1"
                     /translation="MPRDLNSGSHPPNTGGGNGDALSVYDVTPFTMLDFPEKCAAIIW
                     FSGCNMRCGYCHNPQIVKGKGTRKIEDVLTFLEKRASLLDGVVLSGGEASIYPGLAGF
                     AARVKDMGYAIKLDTNGTRPDMVQGFLEQGLLDYVALDYKAPPEKFKTVTGIKKFHNF
                     EKTLDLLCVQSAVPFEVRTTVHTDLMDEYDVSTIIADLENRNYKGVYYIQNYIDNCGQ
                     TLGMLGPQKRTLVLSLLPASTAFSIEFRNFP"
     gene            complement(9868..11559)
                     /locus_tag="PK513_04285"
     CDS             complement(9868..11559)
                     /locus_tag="PK513_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOO81699.1"
                     /translation="MTEQNYPWLETYPPKPDWHMEISPGLMTEVFDQTAARSPNTQAF
                     DFLGKTYDWAALHAAVNKFAAGLQALGVTKGAKVGLFLPNCPLSLIAYYGILKTGGTV
                     VNFNPLYARDELAAQIEDSETDIMVTLDLAMLMDKMEEMLSDTRLSTLIVGTFTDYLP
                     FPKNLLFPIVKSKDIARLEASPRIILLGDFLKNDGQVKPVEINPLEDIAVLQYTGGTT
                     GTPKAAMLTHMNIYANAVQAYANFPDPQGHEKMLAVLPFFHVFAMTVCLNMAVLAGME
                     IVTIPRFDLNDTLDIIQKKKPNYMCGVPAIFSAINNHPKLKNFDLSSLDFCISGGAPL
                     PVEVKKKFETKTGCTVVEGYGLTESSPVATVNPPFGENVAGSIGLPLPQTIIEIIDPD
                     DQTTPMVLGERGEVCIRGPQVMKGYFKRPNETANVLRPTDDGGLRLHTGDIAYMDERG
                     YTYIVDRIKDLIITNGYNVYPRHVEEAIYKHPNVEECIVAGLPDPNRGEIVKAWIKLK
                     EGHELTAEDLKSFLDEKISKIEMPKRIEFRDKPLPKTMIGKLSRKDVVAEELGEV"
     gene            11794..12303
                     /locus_tag="PK513_04290"
     CDS             11794..12303
                     /locus_tag="PK513_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOO81700.1"
                     /translation="MSATQSDLRPEGELQPIKMHLKWFNGTKGFGFVVPEDESFDAFV
                     HITTLQEAGLHALGEGAILMCTLFDGPKGKQVQDVIEVIESGSVNLMPEEIGEDGTFS
                     LGGLVKWYKPEKGFGFIIPDDGMKDVFIHKTLLDKLEMEDLEAGQRVKVRLKVVDKGR
                     EAVDIEIIR"
     gene            complement(12377..12871)
                     /locus_tag="PK513_04295"
     CDS             complement(12377..12871)
                     /locus_tag="PK513_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HOO81701.1"
                     /translation="MSWTDERVELLTKLWGEGKTAAEIAKELGGVTRNAVIGKAHRLK
                     LSNRVSPIQQNKKPAPVKSQPTVAAPERKIQKAAAQDNRQGVPLVDLKPNQCRWPFGD
                     PRDESFGFCGHANIAGLPYCSEHAKVAYQAATRNRILKAEEEAAKEDISQEDLEIVKQ
                     AAAK"
     gene            complement(13031..13942)
                     /gene="era"
                     /locus_tag="PK513_04300"
     CDS             complement(13031..13942)
                     /gene="era"
                     /locus_tag="PK513_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOO81702.1"
                     /translation="MSTDRSEETRCGFVSVVGLPNAGKSTLINQLVGTKVSIVSSKVH
                     TTRSRILGIALHDQAQIILIDTPGIFDAGGARTMEKAMVASALDALEDSECVIHIVDA
                     ASKNTLNKNANLIKKLPKNKPVILVLNKTDKVHKPDLLALATSFNDAFDYAATFMVSS
                     LKGQGTKELLDHLAAELKPGPWMYDEDQITDLPMRMMAAEITREKIFNQLHKELPYSA
                     MVQTETWEEFDNGSIKITQLVFVERDSQKAIVLGKGGTRIKQIGKAAREEMQDIFGMP
                     VHLKLFVKVEEKWAERSENLREMRLLS"
     gene            complement(13942..14622)
                     /gene="rnc"
                     /locus_tag="PK513_04305"
     CDS             complement(13942..14622)
                     /gene="rnc"
                     /locus_tag="PK513_04305"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOO81703.1"
                     /translation="MSVKPVTELEIRIAHHFQDKDLIRAALTHSSAAAGYNYERLEFL
                     GDRVLGLVIAELLYTRFPKEPEGDLAKRLAALVQGSFLAKISQELELGAYITLSDAEA
                     QAGGAENDNILADIFESLIGALYLDGGFEKCQKLIHKLWADRLDVMKEPPQHPKTQVQ
                     EWTQGQGLGLPEYRIVAQSGPDHAPVFDIELRVEGFDPVVAQGKSRQFAEKEAAREFM
                     KQRKSGVS"
     gene            complement(14622..15482)
                     /gene="lepB"
                     /locus_tag="PK513_04310"
     CDS             complement(14622..15482)
                     /gene="lepB"
                     /locus_tag="PK513_04310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO81704.1"
                     /translation="MSQDNFKNSEASLRADPNLKVGADGSEPPLTAKEERADLFKTAM
                     IAVLLALVIRTFLFEPFNIPSGSMKPTLLVGDYLFVSKPAYGYSRYSFPFGLAPLEGR
                     VLDKEPQRGDVIVFKLPSNPRIDYIKRLVGLPGETVQVRNGRLYINGELIPREAIGIK
                     EGTPPEDEGTPMYHYIETLPGGVVHDIFEESDSGPLDNTQVYTVPEGHYFMMGDNRDN
                     SQDSRVQNVVGFVPFENFVGRADFLFFSTNGKAQLFEIWKWPWTLRYNRFFNDIDPVR
                     LTPEAEDSQS"
     gene            complement(15535..>15903)
                     /gene="acpS"
                     /locus_tag="PK513_04315"
     CDS             complement(15535..>15903)
                     /gene="acpS"
                     /locus_tag="PK513_04315"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOO81705.1"
                     /translation="RVQELMARFETRFIERCFTAHERETAEARRSGGGHIAAYAKRFA
                     AKEAVAKALGSGFSGGITMKDIGVVTGERGEPRIQLSGAAQKRLAAITPGGHETFIHL
                     ALSDEPPLAQAVVIIEARSA"
BASE COUNT         4078 a         4022 c         3762 g         4041 t
ORIGIN      
        1 ttaaaccaat gctctgatgt aagaaaaccc gctgataaag tcaacgggtt ttttgaattt
       61 caaacgtgga atgaacggtt aaattctaac cgttattacc agtattgtct aaagaagaaa
      121 cgtgatccat tatctgtagt tctgccattc ctaaagttgt atcactatct acaggaagaa
      181 ggccactatg ataaggaatg gagcgggcga ccgggatcga accggcatat tcagcttgga
      241 aggctgatgt tctaccattg aactacgccc gcattgggat gccagtatac tcattcaccg
      301 gccgttgaca acatattatg gagcgggcga ccgggatcga accccctcgc gcaacgccgt
      361 tgcgcgttcc ttataataaa atagcgggtt cgatacgact cctgatgctg gccggtataa
      421 aaaatactct ccacttcaat taagcgttgg agcgggagac ggggatcgaa cccgcggccc
      481 tcagcttggg aagctgatgc tctaccactg agctactccc gcgtggtggg tagaaataat
      541 ctgatttaga tctgaaatca agtccttcgt cggctgcgcc gacataatct cgtctaagcg
      601 ttcgctttgc tcgcgcaagt ctcattaaaa atcaaggaat tcgaaattgt tttcgcaaaa
      661 ataatcaatc agggcacata catcttcaaa aataccgggc tgatcgtgca gaagaaaaat
      721 gtgaggatga tctgccactt ttagtccctc atcttgttct gcgcgggcga ttagatcttc
      781 gcgggttttt ataggctggc gtccgcggtg cgtatcattg agcatccagt cccgagcagt
      841 gaaggtataa aggcaatcaa cagtatttga agggtaagac gacggcatat cggcaaaggg
      901 ctgtgaaaaa ccctgatcat gcaaatattg ctggaggatg ctggttttgt tgttctcaac
      961 aatgccggcg ctatcaatct gttcaatctt gcagccgtca ccgcgatcaa tataaggaaa
     1021 tcggtaatac tttgcgggac gcgaaatgcc acaacgtgca taggccgctt cgatcaaatg
     1081 atcgcatttt tcaagatcat ccgcccagtc ctgcggctgc atatctccgg caggcttatg
     1141 agcataagaa tgattaccga tccgcaggcc cttagtaatt gcatactcaa tggcgctcgg
     1201 attttgctcc agcaacgccc cgcggacaaa caaaagcgct ggaatgtggc gcgcggtaag
     1261 aaaatcaacc agcttttccg tatcgggcga tgggctgtca tcaatcgtca aataggcctt
     1321 aggcgcactc atgcgttcag ccctttcaga gccggatagg gatatgtgat gtgatagatt
     1381 ggcatggcgc gcagatcacg ggtgacaggc gcaatacctt tgaggcaaaa ccaatgcttg
     1441 aaatgttctt gatcaaattg cccggcctca atcaatcgcc ctaaaacccc gccagtgagg
     1501 taaagcccgc cataagcatg gccggcaatg acactggagg cggcaaacag acccaaaaat
     1561 tcatggaaca ggcgcagagc tgtgctcact tttaaatcat ccgcttggcg catcatttct
     1621 tctgggcttt gcacttgcac tgaactgccg ccaatcaaac acaaagcccg gtaaagatta
     1681 taaagccccg ggccgcttac tacattttca tagacaggaa tggtctcatt acctttaaag
     1741 cgttgaacag cctgaattac aagagcttgc tcatcggtta aacaagccac aggcaagtgc
     1801 ccgccgtgtg taggctgcac aattggtttg tcaccttgat gcacataggc cagccccagc
     1861 cccgttcccg gaccaagaat gcattttgac ggacttgcag gagcgcccgc gcggagagct
     1921 ttcaggtcgc attcttccaa aaccggcaac gaccaagcag ccgcttcaaa atcattgaca
     1981 atgcgttcga cctgccagcc ttcgccttca agctccttgg ggtcaataac ccatgtgttc
     2041 ttattcacga acgcccacag gccgttttcc ggtgaacccg ccgccgcaac ccggagtgca
     2101 ccggatggat ctaattcatg ctctttgcag taatgcgcca gtgcctcttg cagattttcg
     2161 aaatccgccg cttcatattt tgcaatcttt tccggacctt tcggcgaaga gaccgcaaaa
     2221 cgtgcgtacg tgccaccaat atctgccaaa atgctcatga cattatcatg cacgaagcaa
     2281 tgcgttttga aaataatgat ttattggtct atccgccttg gacccaggtt tgcagggcat
     2341 agccgacgcc ataggccatt gccgctgccg tcaagccgat gaataatgtc tcaaacccct
     2401 cacggggcaa agatttcaag gaccacaggc ttttgagaga acctatagca aagaacgtga
     2461 tagccgaaag gatgagagcc aagacaggct gatatgatgt tggcaggatt gcgcccggca
     2521 gcaaaaacgg aatcagcggc atcgcgccac agataaaaaa tgcccagaaa gtttgtacgc
     2581 ccgctttcat cgccgagtgt atgggccggg ctatgccgta ttcttcctga agcataaatt
     2641 caatccagat atctttatct ctggaaatgg ttgataccat ttgctccagc tcttcccctt
     2701 taaaaccttt gctggcgaag atctggcggg tttcctcgat ttcgccatcg tggtgctgtg
     2761 caacatggcg cacttcacgc gcatgcaggc gtttataatt gtcttcttcc gccttcatag
     2821 cgctataagc gcttgcggcc atcgaaaacc cgtcggccag cagattggcc agccccagaa
     2881 ccatgacaat cgcaggagac aaacctgcgc caacaacgcc cgcaacaacg gcgaaagttg
     2941 tcacaacccc gtcaatgccg ccatagaccc attccttaag ataaaaaacc cccggcccgg
     3001 cgctgagacg tgctttgatt tcttccgggc tatgatgatg gtctttaagc atgaaacgga
     3061 tttttctttt caaatgctga agcgtgatta caatgcagtc atgagcatac aaaaccgcta
     3121 tgacaggcaa cgcattctat cggagatagg cgctgctggc caaacgaaaa tttcgacctc
     3181 gtccgttttg tgtgtcggtg cgggcggtct ggggtgtccc gcactattgt atctggccgc
     3241 agcgggagta gggctcatcg gcatcgttga ttttgacgtt gtagaagaaa caaatcttca
     3301 aagacaggtg ctttttgaca caagccaaat tgaccagaac aaggcgcagg ctgctaaagt
     3361 gcgcctaaac gcccttaatc ctgaaattga tattcaggca tacgcggaag agctgagtga
     3421 taaaaatgcg ctcgctcttt ttgaaaaata cgacattatt attgatggga cggacaattt
     3481 tccagctaaa ttcctgatta gcgatgctgc tgttaaggcg ggaaaacctt ttgtttacgg
     3541 atctatcctc ggctttgacg gccagctcgc cgttttcaat tataaaggtg gtccgtgcta
     3601 ccgttgttta ttccccgaag ccccgaaggg gcatattccc aattgtgccc aggccggcgt
     3661 aatcggcgcg gttgccggga tgatcggcac agcgcaggcg atggaggcta tcaaagtcat
     3721 tgtagaccat gaaagcttta aaccccttgc cggtaagctt tggacaatcg atatgcattc
     3781 catggaaaat cgtttactaa ccttgccaaa aacatcagat tgtccagtct gttcaaaaac
     3841 caaagaggag attgccttga attacaattc ccccacttgc ggtcttattc ccgaaatcac
     3901 cccgcagcag gcaaaggaca aacaaaccgc gcttcttatt gatgtacgcg aaatcgacga
     3961 atggaatgcc ggacatattg aaggcgcgca gcatgtcgcc ttatcggttt tagtccaagg
     4021 ccacgttccg gaattgccgc cggattgtga gattgttcta tattgtcaaa aaggtatgcg
     4081 gagcttacag gcagcacaga tccttaaggg ccatggctgc accaacatca tgaatatgac
     4141 cggtggctac gaagcttggc tcaaaagctc ttaaaaaccg ccctttattg actcgcatca
     4201 agtttgtatt tttcaaaata ctgttatagt gtctcgcata attatactta aagagttcgg
     4261 ataattgcga tgaaacacga cattattatc attggcgccg gcccagccgg gttgagcttt
     4321 gcccgcgaaa tggccgaaac ggggctaaat attgttttgc ttgaaaaaca gcccgaaagc
     4381 gttctggcca atccgcccta tgatgggcgt gaaatcgcgc tcacccatct ttcacgaaaa
     4441 atcatgggcg atctggggat gtgggactca attccggatg aaaaaatttc cctgattaaa
     4501 accgccaaag tcctgaatgg cggctcgcct tatgcgctgt ctttcgatca tgaagaggcg
     4561 ggcgaagaga cactgggctt catgatttca aaccaccaaa ttcgaaaagc agcttatgaa
     4621 tcgctggccg gatataaaaa cattcaaatc ctgagcgaaa aagaagtctc tacgcttggc
     4681 accgaccagg aaaaaggttg ggttgagcta agcagtcgag agcgcctgga ggcgccgctg
     4741 atcgtcgccg cagattcccg tttttccgcg acgcggcgta tgatgggaat ttccacttcg
     4801 atgctggatt ttggccgcac ctgcattgtc tgcacgatga gtgcggaatt accgcatcat
     4861 gaaaccgctt atgagtgttt ccattttgac cgcacgctgg ccgtgttgcc tctgaacaac
     4921 aaccaggttt ccattgtcat tacgctgggc tcggaagaaa gcgcgcaagt gcttgcgatg
     4981 ggtaaagaag acttcgctgc cgatatacag caccgttttg aagggcgttt gggcacaatg
     5041 aaactcacaa gcgaactgtt cccttatcct ctggtggcca cattcgccaa gactttctgc
     5101 gccaaccgtt ttgtggttat cggcgatgcc gccgtgggca tgcacccggt cacagcacac
     5161 ggctttaatt taggtctgcg cagcgcacac actttggcta aagaaatcaa agacgctttg
     5221 caaacaggca gcgattacgc ggcgcaaact gttttacagc gctatagcca gaaacacaca
     5281 cacgcctcag cgctgctttt ccatggtaca aatgcgctgg taaagctcta cacaaaagac
     5341 acgcctgcgg ccaaatttgc tcgccggacc ttgctgagat tggggaaccg ccttaaaccg
     5401 gccaaacgtt tgattatgaa tcagctcacg gaaaataagg ctgcttaaag cctatccttg
     5461 aatccgacca taaagaatgt gccatgtaac ctgcccgtga aagctttcgt cggcatatcg
     5521 gcaggttttt agtaagtcct tgcggctgag agttttatat ccgatacgtg gcgtattcat
     5581 tcctcctttt ttcagattac gcaagaaatg cagtgtactt tggtgatgtt caacgatttc
     5641 atcttcccga aaaacaccgg gccaggattc aaaatttagc aatccgttgt ctaaagaaaa
     5701 ttccttcaga cttgcgcgcc attccggaaa actctctggc gcgggcaccg tataaaaaat
     5761 tacgcccccg gattcaagca aacccaaaag cgtttcaaat gtgccatgcg gattaccaaa
     5821 ccactgaaac gccatgttgc tgacaattat atcatagtgc tgatctttcg ccggactttc
     5881 gccatccatt acgctccagc gcacattttt ttgttccccg atgcgcgagc gagcgcgatc
     5941 aaccatgtgc gcacttatat ccgtaatgtg aaaccgcccg ccgggatagc acttcaaaag
     6001 ccgctgcgtc aaagcccccg ttccacaacc gatctccagc accaacggat tttcaatatc
     6061 cggtaaatcc gcggccaggg tctgcgcaat tttttcctga atagcgctat ggcgatcata
     6121 ctcattggcg caatcatcaa atctatgcgc aacgtttttc ttccactcaa agctcacgaa
     6181 taaaatcctt tatatgctcc gcgcaccagt ctgggtggct taaggggagg gcgtggcacg
     6241 caccctcgca aacaggcatg tcatagcccg cccattcttc tttcatgagc gcgagcggca
     6301 aaattgggtc ctctgcccca gccaaagaca agatggggca cgttaaatct tttagctctt
     6361 cttttacatc ccatgaggaa agccatgcca gaccttcttt aagcctttga acgttcaaat
     6421 catcgctggg cggaaggccg cttgttttcc aaaagttttg catctgtttt tccggggttc
     6481 tttgaaggcg cgtcttcatg gcacgcaagg ttcgttcatc cgcaaaatgt tgaaagcagc
     6541 taaaaccgtt aatcgcaata agccccttta cgctccggtg atgattcttc aaagcccaca
     6601 tcgttcctaa agaatgtgtg atgtaaatgg atgactcgcg tgtgatcgcc gtgcaggagc
     6661 tttgcccccc gccaataaac tccagatcaa tcgccactgt ctgcatttca ggcaaacgtg
     6721 cgcgtaccct atcccaaaac gctgagcctg tcccccagcc atgaacaaaa ataatatctg
     6781 cgctcaaccg ctacctcttc taaggcattg aataaacacg cccttgttta cattcaagtt
     6841 ttatattcaa gaaaactatt taaaaattga cctggatcaa atttaaaaca ctatatatag
     6901 tggtaatgtg attcttgaaa atgcaatata ttgtgttttt cctagggttt tcgcccgctt
     6961 tcaaatctta ttcaaaacca atgtaacagg agtttatcat gaccattatt gctgccctca
     7021 aacctgaaat atccgatgaa atcacctcat tatacggtgg tacggacgtt gattgcagcg
     7081 ctgcggtcag tgagtatata tcagaatcag attggcgcgt taaagccaac gccaatacag
     7141 gctattccaa tgccggactg attaacaatg ttgccgggaa ggttatcgcc aatttctggc
     7201 tcgatgaaat ttacgcacct gaagaaggtg gcgcccaccg caaaggcgat atccatatcc
     7261 acgatctgga ttgtttaacc ggctattgcg ctgggtggtc tttgcgtgcg cttttgaacg
     7321 agggtttcaa cggcgttgat gggcgcgttt cgtctcgccc gccgcgtcac ttccgtgaag
     7381 cgctggggca aatgagcaac ttcctcggca ttctccagtc cgaatgggcg ggcgcacagg
     7441 cgttcagctc ttttgacacc tatctcgcgc cttatgtttt tcgtgacaat ctatcattta
     7501 gtgaaattaa aaaagccatt cgccagtttg tttacaatct caatgttccg gcgcgctggg
     7561 ggcagtctcc ctttacaaac attacgctcg acatcaccgt accggaagat ttagccgatc
     7621 aaattccaac caacaacaat ctccatttgt tcaaagggct ggatgatgac gatctgctgc
     7681 gccgtgccca gcaacgcgac atggggctgc gctcgcttga agacaccagc ttcaaacact
     7741 tccttccgga aatggaactg atcgtaattg catattacga agtcatgacc gaaggcgatt
     7801 ccagcggtaa accctttacc ttccccattc caacggtaaa tattactgag gattttgact
     7861 gggactcgaa agttgcccag gccattttcg acaacacagc gaaggtcggt tcgtcctact
     7921 tccagaactt tgtgggttcc cagtggacgc gtgataaaaa cggtgagcgc gtgcgtaatc
     7981 ccgaagcgta ttcccccggc gcggtgcgct ccatgtgttg ccgtttgcaa ctggatctgc
     8041 gcgagcttct caagcgcggc aacggcctgt ttggatcggc ggaaatgacc ggctctttag
     8101 gcgtggtcac gctcaacatg gcgcgtctgg gctatcgttt caaaggcgat atggccgggc
     8161 tgtataaaga actcgaccgc ctgatggata tttcaagatc cacgctggag aaaaagcgcg
     8221 cctatgttca aaaaatgtat gatcgtgggc tttaccctta cacggcacgc tatctaccat
     8281 tctttcgcaa tcatttctct acgatcggcg taaatgggat gaatgaaatg atccgtaatt
     8341 ttaccgaaga tcaacatgac cttacagatg aacgcggcat tgaactctcc atggaaatcc
     8401 ttgatcatat gcgcgcgcgc atggtcgagt atcaggaagc caccgggaat ctttataatc
     8461 tggaagccac gccggcagaa gggacgacat accgttttgc caaagaagat gccaaacact
     8521 atccaggtat tcttcaagca ggggcgggcg aaaatatcta ctatacgaac tcttcccaaa
     8581 tcccggctgg tcatacggat gatccgttcg aagcgctgga gctgcaaaac aaactgcaat
     8641 gcaaatatac aggagggaca gtactgcatt tgtatatggg cgaaaaactt tcttccgccg
     8701 aagcctgcaa acgctttgtc aaaaaagtga tcgagaacta ccaacttccc tacatcactg
     8761 ttacgccagt attttcgacc tgcgagacgc atggctacct caatggcgaa cagcccgaat
     8821 gcccgcattg cggtgaggaa acaaacgtct ggacgcgcgt catgggctat ttccgccccg
     8881 tggccagctt taataccggc aagaagggcg aacacaaaga gcgcaaacac ttccttgagc
     8941 gccgcatcga atctgccgat ctgtttgaga aggcctgaac atgccaagag atttaaattc
     9001 aggaagccac ccccctaaca cagggggtgg caatggcgat gcgctctccg tttatgacgt
     9061 tacgccgttt actatgctcg attttccaga gaaatgcgcg gcgattattt ggttttcggg
     9121 ctgcaatatg cgttgcggct attgtcacaa tccccagata gtaaagggaa aaggaacaag
     9181 gaagatagaa gacgttttga cgtttcttga aaaacgcgcc agcctgctcg atggcgttgt
     9241 tttgtctggc ggtgaggcct ctatttatcc cgggcttgcg ggttttgcag cccgcgtgaa
     9301 agatatgggt tatgcaatca aacttgatac aaacggcaca cgccctgata tggtgcaagg
     9361 ttttttggag caagggcttt tggattatgt tgcgctggat tacaaagcgc cgccggaaaa
     9421 gttcaaaact gtgacaggta ttaaaaaatt ccacaatttt gaaaaaacac tcgatttatt
     9481 atgcgtgcaa agcgccgtgc ctttcgaagt ccggaccacc gttcacaccg acttgatgga
     9541 tgaatatgac gtgagtacga tcatagcgga tctcgaaaat cgaaattata aaggcgtata
     9601 ttacattcaa aactacatcg acaattgcgg tcaaactctg ggaatgttgg ggccgcaaaa
     9661 gcgtacgctg gttttgagtc ttttgcctgc cagcacggca ttttcaatag aatttcgaaa
     9721 ctttccctga aactctcagg gacttattat gttatatttt aaacacggaa agggtgggat
     9781 gtttgtggag tgctccgctt gcaataacag agatataaga cgtcattgcg agtaagggcg
     9841 atgcccggag cgatgacctg tttttggtta gacttctccc aattcctccg ccacaacgtc
     9901 cttccggctg agctttccga tcatggtttt gggcagcggc ttatcacgaa actcgattcg
     9961 tttgggcatt tcgattttgg aaattttttc gtccagaaag cttttcagat cttctgccgt
    10021 caactcgtgg ccctccttaa gcttaatcca ggccttcacg atttccccgc gattaggatc
    10081 aggcagcccc gcaacaatac attcttcgac attgggatgc ttatagattg cttcttcaac
    10141 atggcgcgga taaacgttgt agccgttggt tataatgaga tctttgatcc gatcaacaat
    10201 ataagtatac ccccgctcat ccatatatgc gatatcaccg gtatgcaggc gcagcccgcc
    10261 atcatccgta gggcgcagta catttgccgt ttcgtttgga cgcttgaaat agcccttcat
    10321 cacctgcggc ccgcggatgc agacctcgcc acgctccccc aaaaccatcg gcgtcgtttg
    10381 atcatccgga tcgataatct cgataatggt ttgcggcagg ggtaaaccaa tggaaccggc
    10441 cacattttca ccaaacggcg gattaacggt agcaacagga gaagactcgg tcagaccata
    10501 cccttccaca accgtacatc ccgtctttgt ttcgaatttt ttcttaactt caacaggcag
    10561 cggtgcaccg ccggaaatac aaaaatccag cgaagacagg tcgaaatttt tgagctttgg
    10621 atggttgttg atggcgctga aaatcgccgg aacgccgcac atatagttcg gttttttctt
    10681 ttggataata tcgagcgtat cattcagatc aaatcgcggg atggtcacga tttccatgcc
    10741 ggccagcaca gccatattca aacaaaccgt catggcaaac acgtggaaaa acggtaacac
    10801 ggcaagcatt ttttcatgcc cttgcggatc aggaaaattg gcataggcct gcacagcgtt
    10861 ggcataaata ttcatatggg tcagcatcgc cgccttgggc gtgccggtcg taccgccggt
    10921 atattgcaac acggctatat cttcgagcgg gttgatttcg acaggtttta cctgcccgtc
    10981 atttttgaga aaatccccta gcaaaataat gcgcggtgaa gcttcaagcc gggcaatatc
    11041 cttgcttttc acgatgggaa aaagcagatt ttttggaaaa ggaagatagt ccgtaaatgt
    11101 cccgacaatc agcgtactca gccgcgtatc ggacagcatt tcttccatct tgtccatgag
    11161 catcgccaga tcgagcgtga ccatgatgtc ggtttcactg tcctcgattt gggcggcgag
    11221 ttcatcacgt gcgtagagtg ggttgaaatt gaccaccgtt ccaccggttt taagaatgcc
    11281 ataataggca atcagcgaga gcgggcagtt gggtaggaat aaaccaactt ttgcgccctt
    11341 ggttacgccg agggcttgca atccggcagc aaatttattt acggcagcat gcaacgcggc
    11401 ccagtcatat gttttaccca gaaagtcaaa agcctgcgta tttggtgagc gcgccgccgt
    11461 ttggtcgaag acttccgtca tcagacccgg agaaatctcc atatgccagt ccggctttgg
    11521 tggataggtt tcgagccaag gatagttctg ttcagtcatg agcatacctg tgtgcaaaaa
    11581 tcacacttta attataactg tttttcgcaa ggctttctat gcgttatgca atattggctg
    11641 gttcttagaa aaaacataac ctttatggtt gtattcaatt ttataaaaca agcctctaga
    11701 tttttactaa aagatatagc acactatatt tgggtttgtt cctaagcctg tccgcttagt
    11761 cttagttatt gtgttaattt ggagaattcc ccattgtctg ccacgcagag tgaccttcgt
    11821 cctgaaggtg aacttcaacc gattaaaatg catctgaaat ggtttaacgg aaccaaggga
    11881 tttggttttg ttgtacccga ggatgaaagc tttgacgcgt ttgtgcatat cacgactctt
    11941 caagaggcag gtctgcacgc actgggtgaa ggtgccattt tgatgtgcac attattcgat
    12001 ggtcccaaag gcaagcaggt tcaggatgta attgaagtga tcgagtccgg cagcgttaat
    12061 ctgatgcccg aggaaatcgg tgaagacggt acgttttcgc taggcgggct tgtaaaatgg
    12121 tacaaaccgg aaaaaggctt cggctttatc attcccgacg atggcatgaa ggatgtcttc
    12181 atccataaaa cgctgcttga caagctggaa atggaagatc tcgaagccgg ccagcgcgtt
    12241 aaggtgcgcc tgaaagttgt cgataaaggc cgcgaagcag tcgatataga aattattcgg
    12301 taaaaatcgt aaatctcaat catataaaaa agggcgcttt tttcgcgccc tttgtcattc
    12361 atacataaag ctgaagttat tttgcagcgg cttgttttac gatttcgaga tcttcctgag
    12421 agatatcctc ttttgccgct tcttcttcag ccttaagaat gcgattacgg gtcgcagcct
    12481 gataagcgac cttggcgtgc tcggagcagt aaggcaggcc ggcaatgttc gcatgcccgc
    12541 aaaagccgaa actttcatca cgcggatcgc caaaaggcca gcgacattga ttgggtttga
    12601 gatcaacaag cggaacgcct tggcggttat cctgcgcggc agctttttga atttttcttt
    12661 cgggtgctgc tacggtcggt tgtgatttta ccggagcggg ttttttattt tgctgaatag
    12721 gagaaacgcg gttggaaagt tttaagcggt gcgctttacc gataacggca ttgcgcgtaa
    12781 cgccaccaag ttccttagcg atttctgcgg ctgtctttcc ttcgccccag agtttcgtca
    12841 gcaactcaac gcgttcatca gtccaactca tgcttttttc tcccactggc gcttgcacca
    12901 gataagtcac tgatacaaaa cattaaaatt aactcgattt tctctagaga ctgcagtcta
    12961 tcagcagcga atcattttgg tcaaattatg atgagaaaaa aaatgcgtta tccacatcct
    13021 gaatttattt tcaggaaaga agcctcattt ctcgcaaatt ttcgctgcgc tcagcccatt
    13081 tttcttccac tttgacaaag agtttgaggt ggacaggcat gccaaaaata tcctgcattt
    13141 cttcacgggc agcctttccg atttgtttaa tgcgcgtccc gccttttcct agcacaatcg
    13201 ccttttgact atcgcgttca acgaacacaa gctgggtaat tttgatgctg ccattatcaa
    13261 actcttccca tgtttcagtc tgaaccatcg cggagtaggg cagttcctta tgaagctggt
    13321 taaagatttt ttcgcgggta atttccgccg ccatcatacg catcggcagg tcggtaatct
    13381 gatcttcatc atacatccac ggccccggtt taagttcggc ggcgagatga tccagcagct
    13441 ctttcgtgcc ctgacctttc agagacgaaa ccataaaagt ggctgcataa tcaaaagcat
    13501 cgttaaagct tgttgccaaa gccaacaggt ccggcttgtg aaccttgtcg gttttattga
    13561 gcaccagaat aacgggctta tttttaggaa gctttttgat cagattggca tttttgttga
    13621 gcgtattttt gctggccgca tcgacgatat gaatgacgca ttcgctatct tccagtgcat
    13681 caagcgcgga agccaccatc gctttctcca tcgtgcgcgc ccctccggca tcaaaaatgc
    13741 ccggcgtatc aatgagaata atttgcgctt gatcatgcaa tgcaattccc agaatgcgcg
    13801 aacgggtcgt gtgcaccttg ctggaaacaa tcgagacttt cgtgccgaca agctggttaa
    13861 tcaacgtaga tttcccggca ttgggaaggc ccacaacact cacaaaaccg cagcgcgttt
    13921 cttctgatct gtcagtgctc atcaggaaac cccgcttttg cgctgtttca taaattcacg
    13981 cgcagcctct ttttctgcaa actggcgcga cttgccttgc gcgaccaccg gatcaaaacc
    14041 ctcaacgcgc agttcaatat caaagacagg cgcatgatcg ggcccgcttt gggctacaat
    14101 tctatactca ggcagcccca atccctgacc ttgtgtccat tcctgcactt gtgttttggg
    14161 gtgctggggc ggctctttca ttacatcaag gcggtctgcc cataatttat ggatcagctt
    14221 ttggcatttc tcaaaaccgc cgtcgagata taacgctcca attaatgatt caaaaatatc
    14281 ggccaggata ttgtcatttt ccgccccacc ggcttgtgct tcagcgtcag aaagcgtgat
    14341 gtaagcacca agctctagct cctgagatat ttttgccaga aaagagccct gcaccagcgc
    14401 cgccagccgt ttggccaaat ccccttcggg ctctttggga aaacgcgtat agagcagctc
    14461 ggcaatcacc agccccaata cgcggtcacc aagaaattca agtcgctcgt aattataccc
    14521 ggccgccgcg cttgaatggg taagcgcggc gcgtatcaaa tctttatcct gaaaatgatg
    14581 tgctatacgg atttcaagtt ctgtaacagg tttaacactc attacgactg ggaatcctcg
    14641 gcctctggcg tcaggcgcac ggggtcgatg tcattgaaaa aacgattata acgcagtgtc
    14701 catggccact tccagatttc aaaaagctgc gccttgccat ttgtcgaaaa gaacaagaag
    14761 tcggcccggc caacaaaatt ttcaaagggc acaaagccga caacattctg tacgcggctg
    14821 tcttgtgaat tgtcgcgatt atcgcccatc ataaaataat gcccctccgg aacggtataa
    14881 acctgagtgt tatcaagcgg cccgctatcg ctttcttcga aaatgtcatg aacaactccc
    14941 ccgggcaggg tttcgatata atgatacatc ggcgtacctt catcttcggg cggcgtacct
    15001 tcttttatgc cgatagcttc gcgcggaatg agctcgccat tgatgtaaag gcggccattg
    15061 cgaacctgca cagtttcacc gggcaaccct accagacgct tgatgtaatc aatgcgtgga
    15121 ttgctaggca atttaaagac aatgacatcc ccgcgttgcg gttctttatc caacacgcgc
    15181 ccctccaaag gtgccagacc aaaaggaaag gaatagcggc tatagccata ggccggtttg
    15241 ctgacgaaca gataatcgcc aaccagcaaa gttggtttca tggacccgga agggatatta
    15301 aacggctcga acaaaaaggt gcgtattacc agagcaagca agaccgcaat catcgccgtc
    15361 ttaaaaagat cagcccgctc ttccttggct gttaacggtg gctcgcttcc gtctgctcca
    15421 actttcaggt taggatcagc gcgcaggcta gcttcagaat ttttaaaatt atcttggctc
    15481 attttgtata ttctcgccct tctaaagcgc ttttacagga tttatggacc ggactcaagc
    15541 gctacgcgct tcgatgatga ccacagcttg agcaagcggc ggttcatcac tcaaagccag
    15601 atggataaat gtttcatgcc cgccgggagt gatagccgcc agacgttttt gtgccgcgcc
    15661 gctgagctgt atccgtggtt cacctctttc gcccgtaacg acgccaatgt ctttcatggt
    15721 aatgccaccg gagaaaccgg agccaagcgc tttggcaacg gcttccttag cggcaaaacg
    15781 cttggcatag gcggcaatat gcccgccgcc tgagcgccgc gcctcggctg tctcacgctc
    15841 atgggcggta aagcagcgct ctataaagcg cgtctcgaaa cgcgccataa gttcctgaac
    15901 acg
//
LOCUS       DAOSWR010000037        15773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2338_length_15773_cov_5.946876, whole genome shotgun sequence.
ACCESSION   DAOSWR010000037 DAOSWR010000000
VERSION     DAOSWR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_15773_cov_5.946876"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..3207)
                     /locus_tag="PK513_04320"
     CDS             complement(67..3207)
                     /locus_tag="PK513_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOO81706.1"
                     /translation="MAENAALAKVAGGADSQEESFTQTERLSFAKEFLTRMRRGFATA
                     DKFLQNASPATKSGASSAQNILPEGEQLLFEVYLPRRLKLDNLIFGRVKGQRIALSLK
                     DFVAALDLPITVNTQTQTAQGWYIRENKFFDFNLNTKIARSDLGEFTISESVFSEDED
                     IFVPVDELARWFDFKIEVLASSLQIVIKPSQNLPLQERLEREKKLAGKGNSREPQLPL
                     RPEPRQLIDVPFVNVATSSRYNREGDSNQSNTRHTASVSTAGDFAYGTLSTQTQLNDK
                     DQISNIWMNYKQESPDPNLLGPLKAKRFEIGDVIQPRLPIDAQVRRELGARISNVDPL
                     RNYTNPSTAITGRAFPGWDVELYRGNQFLGYQRIGDDGFYAFNDVILFGTENIFRVVF
                     YGPQGEIREEEVSIPVDISRIAEGGSVYDVSLTFDNKQTYRKLDFNDEDEGSPALLAL
                     YEKPIAPGTVGSLGLRSSEQNGERNNVAYGGVSTLLGETLVNANLSLDDEADFASELV
                     ARRNIGSHDLYTTVRYFSDNYDTINGGNDTAGFLNARINANGALPIGLGHRPNYTASA
                     SYNLTNEGNSTTDTSLGFNTAYKNFTFNEQLNYTTTNTGADDRLNNTTSITGAFAGDR
                     VRLLSDYQIKPDNKLSRIVASYNHGFTRDLEMDIELERRIDPSITEASAQLNWQAGWA
                     RISPSIRYNTENDFFAGLNTNFGFARDPQGPGIKMFDRNITGNGAMSVFVFLDMNGDG
                     QFNQGDVPLKDVSVRALQNGGREVTDENGLAFFYQVQELRRTDIVLEEESLKDPYWIS
                     GFDGVSILPREGYVAELQYPVHIAGEMDGTLFVRKNSGKPVALRNIPIHLYNGDGAIE
                     KTAITDIGGFYLFDRVAPGRYLLVVDQKAAQSNNFARPKPEAIEIGYEGTIIYGHDVY
                     VDAGEKDIPSTVLAGLQDYKSRHPHIDFEQANYNIALNLGEYNSQLLMSTVWYRLHTR
                     YRQILSGGQLMVLPEHSYADIKTGKHTLRVGFHNGDIDDAYNRCRALIAREIACTVEV
                     LPAAAKKLAMGEKPRG"
     gene            3510..4085
                     /locus_tag="PK513_04325"
     CDS             3510..4085
                     /locus_tag="PK513_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81707.1"
                     /translation="MKFTSMKALAFAGALTIGLAMSGTAQAQTENVTATLITSSAITS
                     ALVTDMDFGEFLIQHGTDTPTLTLSNDGSIAVTSGGVVDSQVVEITPTISEGGVTVQI
                     PAPGSLDMTSSNLIDFASASLALDIVTYRTASENGQVITTGGVTGVAVPVTVLAGATD
                     EAIAFGGTIDISGTPLDNTHTATFDVTFTYP"
     gene            complement(4167..4856)
                     /locus_tag="PK513_04330"
     CDS             complement(4167..4856)
                     /locus_tag="PK513_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81708.1"
                     /translation="MNTSNEAKTYDIGWQHYRMNEDDGTGAGAATTITESPATEFDLS
                     QYVVFTPRRVTLPPSAKQKIRLALRRPAGVADGEYRAHLQFKALRDDINEQNESQEKG
                     ASSAAVKINISYSIPVMFRAGSPTLAGKIQNVKLQRNPENGMLEALVTIARGNDPYGV
                     IGHMYIYDPTGKVIGENGNAHVYPEVPSRTFRVPLIDESNLSGRNIRIVLRHYDNDKG
                     LIYDERLVPVQ"
     gene            complement(5017..5481)
                     /locus_tag="PK513_04335"
     CDS             complement(5017..5481)
                     /locus_tag="PK513_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81709.1"
                     /translation="MIRHIQYVLAGLILLGLSPRAFAGVTEIQALQFGEYIVTHNDAV
                     YSITVNVGGVVTYDPAGFIEIAATGQDGIYDIDGLPASTAITSVVISQTTPLTSGGLS
                     FQMNTFTESHPASTDGAGVAQIRVGATADTSGNGVPYLDRTYNGMLEIQINF"
     gene            5746..6708
                     /locus_tag="PK513_04340"
     CDS             5746..6708
                     /locus_tag="PK513_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81710.1"
                     /translation="MNKRFGLWVFWMLFAGLTASCGPLTDTPKPEQTLNSAPSSENLI
                     SPNAPSQASSTSALEVPAPIAMPPSYGDRPPEPGQMQSSTMPNGLPALQPKGINVESL
                     FATKISDSDQRFARLENAVLDLRREFETFKPAITRLVAVESDIQDLIKQLDILLRNEP
                     VAPPMPLKPETGPMPLTAADQAPPPNAGAISPAPPQSPAAPPAPKAVSGKPVVKALRV
                     GQHSDKIRLVLDVSKKTPYSADLDTEEKLLIIELPEAQWNAARQQSFARSELLQSYSV
                     EPINDGAGSRVVMTLKRPTQLIKQQALPPGNGNADHRIFLDLSL"
     gene            complement(6725..8092)
                     /locus_tag="PK513_04345"
     CDS             complement(6725..8092)
                     /locus_tag="PK513_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOO81711.1"
                     /translation="MRISFLLTCSALTLSGCIVSSLDLPDFKGPGSWVSASGQEQRIA
                     GANAVSLQNWWYRFNDPLLSVLIDKALADSPDRKIAEARILEARGLRKATRASLFPQI
                     GASATKGREDTVQSDADDYYDARFDVSYELDIFGKNRKRFAASNATLENLEAKYQDVT
                     LSLVAEVARTYTEMCAAEKQVRIAQDNLKTQEQTLSLVDQLYAAGESPQLDVERTKNL
                     VNTTRASIPEFQRQSENARLRLSVLTGALPNDLAGIEFVSKKIPDSDVAPVLMAPARV
                     LALRPDIRAAAANLRAKTNLSAATLTAIFPSFTLSGFYGVAENAFASSTGIWNVAVGA
                     AVTVLSFGRLEGEIDAARAREMEAYESYRKVVLEAVVEVETALSDYAHINEQAFSLFK
                     AYENAQEAFFLSQQLFKEGEISFLDVLDAQRTVNDAQSALTNAQAAKALALIRLYKSL
                     GVGPT"
     gene            complement(8089..11307)
                     /locus_tag="PK513_04350"
     CDS             complement(8089..11307)
                     /locus_tag="PK513_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOO81712.1"
                     /translation="MTLSGYFIDRPIFASVLSLLIFIAGFIAMFNLPISEYPEVSPPS
                     VNVSAAFPGANPMTIAETVATPLEEQINGVENMLYMSSLASADGRMSLTITFEIGTDV
                     DLAQQLVQNRVSQALPRLPDVTRQLGVTVTKSSPDLTMVVHLRSPSERYDMLYLRNYA
                     TINVKDRLAKISGVGRVMVFGSGDYAMRVWLNPEAIAELGMTPGEVIASIRGQNLQVA
                     AGTIGGAPYEEGLQVELPVNTQGRLQSVEEFENIIIKADENGALTRLKDVARLELGAE
                     SYALRSFLNNEPAVAIPIFAAPDANALEISDNVRAAMADLKKNFPDEIDYSIVYDPTV
                     FVKDSIQSVIHTLFEAVLLVVLVVIVFLQTWRASVIPFLAVPVSIVGTFAIMLFLGFS
                     INVLSLFGLILAIGIVVDDAIVVVENVERNISEGLKPRDATIQAMKEVTGPVIATSLV
                     LVSVFVPIAFLSGLTGQFYRQFAVTIAIATIISTIVALTLSPAYSAMLLKTKDAPKDL
                     LTRLINVLFGWFFVIFNKIFTASAHGYSKAVGFFTRHRVIMLILYAGFIAATVFSFNL
                     VPKGFIPMQDKQYLVAFAKLPPGANLERTESVIRQMADIATAHPGVSNAVQFPGLSIN
                     GFISSASSGIVFITLDDFEDRMSPEQYGPAIAGQLQQKFMAIPDAFVAIFPPPAVRGM
                     GTVGGFALHIQDRADLGIEALNKAVQQVTMRANQDPALSYVFSNYDVNYPQLFANLDR
                     ERAQQLGIPIQDVFAAMQVYLGSLYVNDFNMFGRTYQVIAQGDKEYRSDPSDILRLKT
                     RNKNGDMVPLGSVIKVEQSFGPEAAQRYNAFRSADLNGNIAPGYSTGQGQDAIAKILA
                     ETLPPGMSFEWTGLTYQQILAGNTAMLIFPLCLLLAYLVLAAQYENLFLPVAVILIVP
                     MSTLSAMVGVYLFGQFQMSMGAPTGMANNIFTQISLFVLAGLACKNAILIVEFARDLE
                     LQGRKIVEAAIEAARMRLRPILMTSFSFIMGVLPLVTSHGAGAEMRQAIGIAVFSGML
                     GVTFFGIVFTPLFYVVIRKVEVLLTGHNIHKASLESAGDNKI"
     gene            complement(11312..12589)
                     /locus_tag="PK513_04355"
     CDS             complement(11312..12589)
                     /locus_tag="PK513_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO81713.1"
                     /translation="MPKSLLEKLILSKLLIKLTVFCVIMLFVAGLFIALNPFNHADAQ
                     GPGEGMPQGPMPVSVEIIGTQDVRIWKDFPGRLSAVDYAAIRPQVSGRITEILFQDGQ
                     HVKKGDVLFVIDSRPYKAAVEQAKAALQAAQSNYNLADKEYKRAQELIKTNAISQRIV
                     DERRSRFNFVLAEISGAKAALQKAEIDLDYARVKAPISGRVSRAEITLGNVVESGPGA
                     PIVTTIVDDESIYVDFEVDEQTYLSNIREQVRSKEDEGKIPVQVILRGDAEQPIDGVI
                     HAFDNRINPASGTIRARAVLENSDGALLSGMFAKVRLGSADRARRMLVGEKAIGIDQD
                     RRFVYVVNKDNVIEYRQVQLGDRSGHSRVVLSGLSEGEKVVAQGIIMIRPGMPVAPMM
                     AGEQDKAESVPAAAPSEEPPQDETSSSSSIKGP"
     gene            complement(12776..13654)
                     /gene="cysQ"
                     /locus_tag="PK513_04360"
     CDS             complement(12776..13654)
                     /gene="cysQ"
                     /locus_tag="PK513_04360"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOO81714.1"
                     /translation="MSFNPNSMFDAEFHKRARERTRKLLANPEALCNMVRRIALGAGD
                     IIMKYYEDLDGLEVDSKHDDSPVTLADREAETFIQMSLEQLTLDIPVIGEEAVALGKK
                     TSVEGHEYFWIVDPLDGTKEFMNGGEEFTVNIALVKNAKPVLGVVYAPALGELYAGHG
                     EGTAIRWSQDTDTEKPISVRPPPKGGLNVVASKSHGDAQRLEKFLEQFRIKKLIKRGS
                     SLKICVIAAGKADIYPRLGPTCEWDTAAADAVLQAAGGVITDVNGEVLKYGGANPKWL
                     NPEFVACSFEWFNVKE"
     gene            complement(13859..15220)
                     /locus_tag="PK513_04365"
     CDS             complement(13859..15220)
                     /locus_tag="PK513_04365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOO81715.1"
                     /translation="MRIGMVGTGYVGLVSGTCFAEFGIDVVCLDKDEGKIKRLKQGEI
                     PIYEPGLEDLVAKQVKAGRLSFTTDLEEALKDSIAVFIAVGTPARSDNGHADMTYVYE
                     AAREIARVMEGYTLIVTKSTVPVGTAREVKRIMNEELGKQGRTDVEFDVCSNPEFLRE
                     GAAVNDFLRPDRVVIGADSERAVEIMRDLYRPLYINETPMVITSPETSETIKYAANAF
                     LATKITFINEIANLCEKTGANVQDVARGIGLDGRIGAKFLHAGPGYGGSCFPKDTLAL
                     TQIGQKYNAPQNIVETVVWVNTARQRSMAQRVIEACGGNVQGKRIAILGVSFKPNTDD
                     MRDAPSLQIIPYLQDEGAHVAAFDPVAMDEAKKHLSKVEWCKDAYVAATNADALVIVT
                     EWNEFRALGLGRLGEIMKEKRMIDLRNIYKPEDMRERGFHYVSIGRPEVPPGNEAKLK
                     KVS"
     gene            15507..>15773
                     /locus_tag="PK513_04370"
     CDS             15507..>15773
                     /locus_tag="PK513_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HOO81716.1"
                     /translation="MAKKETKKDAPAKAEPKASEQKPAANMPLFYSNPVPLDAKAHAD
                     LALKQNFGFGFTEGVNAVPLNLIEMPQVCHYYPIAFSPDGNATPV"
BASE COUNT         4005 a         3964 c         3788 g         4016 t
ORIGIN      
        1 cgatcgtagc ttaacttcgc cgcaaagctg gccgaagaac cgagaccacc tctctttttc
       61 gtggaattat ccgcgcggtt tttcgcccat agccagtttt ttggcggcgg caggcaggac
      121 ttcaaccgtg caggcaattt cgcgggcgat aagggcgcgg caacgattat aggcatcatc
      181 tatatcgccg ttatgaaagc cgacgcgcaa cgtatgttta ccggttttta tatcggcgta
      241 gctatgttcc ggcaggacca taagctgtcc gccggaaagg atttgacgat agcgggtatg
      301 cagacgatac caaacggttg acataagcaa ctgggaatta tattccccaa gattgagcgc
      361 gatgttgtag ttagcttgtt cgaaatcaat atgcggatga cgggatttat aatcttgcaa
      421 gcctgccaaa actgtacttg gaatatcttt ttcaccggca tcaacgtata cgtcatgccc
      481 atagatgatg gtgccttcat aaccgatttc aatggcttcc ggttttgggc gggcaaaatt
      541 gttcgattgc gccgcctttt gatcaaccac cagcaaataa cgtccgggcg ctacgcgatc
      601 aaaaaggtaa aatccgccaa tgtcggtgat agctgttttt tcaattgctc catcgccatt
      661 atagaggtgg atgggaatat ttctcagcgc aacaggcttc ccagaatttt tacgcacaaa
      721 taatgtgccg tccatttcac cggcgatatg aacgggatat tgaagttctg caacgtatcc
      781 ttcgcgcggt aaaattgaaa caccgtcaaa gccagaaatc cagtagggat ctttcagact
      841 ttcttcttcc agaacgatgt ccgtgcgcct gagctcctgt acctggtaaa agaaagccaa
      901 accgttttcg tccgtaacct cgcgtccacc attttgcaat gcgcgtacgg atacatcctt
      961 tagcggcacg tcaccctggt taaactgacc atctccattc atatcaagaa aaacaaaaac
     1021 gctcatcgca ccgttgccgg tgatgttgcg atcaaacatc tttatgcccg gcccttgtgg
     1081 atcgcgggca aaaccaaaat tggtgttaag gcctgcaaaa aaatcatttt cagtattgta
     1141 gcgaatgctg ggggaaatgc gcgcccatcc cgcctgccag ttgagttgcg ccgaagcttc
     1201 agtaattgag gggtcaatcc gacgttcaag ctcaatgtcc atctcaagat cgcgggtgaa
     1261 accatggtta tagcttgcca cgatacggct gagcttattg tcaggcttaa tctgataatc
     1321 cgatagcaga cgcacgcgat cgccggcgaa ggctccggta atactggttg tattatttag
     1381 cctgtcgtca gcacctgtgt tggtcgtagt ataattgagc tgctcattaa aggtaaagtt
     1441 tttgtaggcc gtattgaaac cgagagatgt atcggttgtt gaattacctt cgtttgtcag
     1501 gttgtagctt gcagaagccg tatagttcgg gcgatgccca agaccaatcg gcagggcgcc
     1561 gtttgcattg attcgcgcat tcaaaaatcc ggccgtgtca ttcccgccat tgatggtgtc
     1621 gtagttatca gaaaaatacc gcacagttgt atacagatcg tgggagccga tattgcgacg
     1681 tgcaaccagc tccgaagcaa aatccgcttc atcatccagt gataagtttg cattcaccag
     1741 cgtttcgccc aacaaggtcg atacacctcc ataggcgaca ttgttgcgct caccgttttg
     1801 ttcgctacta cgcagcccca aggagccgac cgtacctggc gcaataggtt tttcatataa
     1861 agccagcaag gccgggctgc cttcgtcctc atcgttgaaa tccaatttgc gatatgtttg
     1921 tttattatca aacgttaaag acacatcata tacactcccg ccttcggcga tacgcgaaat
     1981 atcaacgggg attgatacct cttcctcgcg aatctcgcct tgcgggccgt aaaaaacaac
     2041 acgaaaaatg ttctcggtgc cgaaaagaat cacatcgttg aacgcgtaaa agccatcatc
     2101 accgatgcgc tgatagccca agaactgatt gccgcgataa agctcgacat cccaaccggg
     2161 aaaagccctg cccgtaattg cggttgacgg attcgtatag ttgcgcaagg ggtcaacatt
     2221 gctgattcgc gcgcccaatt cgcggcgcac ttgtgcatca atcggcaagc gtggttgaat
     2281 tacatctccg atctcaaagc gctttgcctt taacggaccc aataaattcg ggtccggaga
     2341 ttcctgctta tagttcatcc agatattgct tatctgatct ttatcattca actgtgtttg
     2401 cgtactcaat gtcccatagg caaaatcacc ggcggtgctt acgctggccg tgtgacgggt
     2461 gtttgattga tttgaatccc cttcacgatt atagcgcgag ctggttgcga cattgacgaa
     2521 aggtacatcg atcaattgtc tgggctccgg ccttagcggt aattgcggct cgcgactatt
     2581 tcccttgcct gccaattttt tctcacgctc cagacgttcc tgaagcggta agttctgaga
     2641 aggcttgatt actatttgta agctagacgc caaaacttca attttaaaat caaaccagcg
     2701 cgcgagttca tcgacgggaa caaagatatc ttcgtcctca gaaaacacgg actcagaaat
     2761 agtaaattcg cccagatcag agcgcgcaat ttttgtgttt agattgaaat caaaaaattt
     2821 attttcacgg atataccagc cttgtgccgt ttgagtttgc gtattcacgg taattggcaa
     2881 atcgagtgcg gcaacaaagt cttttaatga caaagcaatt cgttgccctt ttacacgacc
     2941 aaaaatcaaa ttgtccagtt tcaagcgcct cggcagatat acctcaaaaa ggagctgttc
     3001 gccttccggc aaaatatttt gcgccgatga cgcgccgctt tttgttgcgg ggctggcatt
     3061 ttgcaaaaat ttatcggcag tggcaaaacc gcggcgcatg cgtgttaaga attctttggc
     3121 aaatgataaa cgctcagttt gcgtgaaaga ttcctcttgc gaatcagccc cccctgctac
     3181 ctttgcaagc gcagcatttt cagccataaa aaaacaactg ataaaacaaa tgtatagcgc
     3241 caatctcaag cgaaaaattg tcataaaact ctaaagctgt tatgggtata tggtgtaaga
     3301 cgcaaagaac tacagccatt taagcacata aagtctgtgg acacaattga acggcttttt
     3361 taaataatct gtgaataggt tttttgttta aaatttaata atgatttaac cggtttcgtg
     3421 tgatattaaa gaggtagtct taatttttct aaaattaaaa gcactgcgtt caaagaagat
     3481 gtaaccaggg actttaaagg agaagcctta tgaaatttac ttccatgaaa gcgcttgcat
     3541 ttgcgggcgc actcacgatt ggtcttgcta tgtccggcac agcccaagca caaactgaaa
     3601 acgtaaccgc cactttgatt acttccagcg cgattacatc agctctcgtc actgatatgg
     3661 attttggtga attcttgatc cagcatggaa cagatacacc aacactgaca ttatctaacg
     3721 atggctctat tgctgtaaca tctggcggtg ttgtcgactc tcaagtcgta gagatcacgc
     3781 cgactatttc agaaggtggt gttacggtgc aaatcccagc cccaggatca ctggatatga
     3841 catcgtcaaa ccttattgac tttgcttccg cttctttggc tttggatatt gttacttatc
     3901 gtacagcctc agaaaacggg caagtcatca caacaggcgg ggtaaccggt gtggctgttc
     3961 ctgtaacggt tcttgccggt gccacggatg aagccatcgc ttttggtggt acaattgaca
     4021 tctcaggtac gccgcttgat aacacgcaca cagctacatt tgatgtgacg tttacgtatc
     4081 cataagcggg taacggtata taaagtttga aaaaggcgcc ctttgaggcg ccttttttac
     4141 agtctttgtt cttccgctta tacagtctac tgcacaggga ctaaccgctc atcatatatc
     4201 aggcctttat cgttgtcata gtgcctgagc acgatgcgta tattgcggcc tgagagattg
     4261 ctctcatcaa tcaacggcac gcgaaatgtg cggcttggaa cttccggata gacatgcgca
     4321 ttaccgttct ccccgataac ctttcctgtc ggatcataaa tatacatatg cccgatcaca
     4381 ccatatggat cgttcccgcg cgcaatcgtt acgagtgctt caagcatgcc gttctctgga
     4441 ttgcgctgaa gctttacatt ttgaatttta ccggcaagcg ttgggctccc cgcgcggaac
     4501 atcaccggaa tactatagga gatattaatc ttcacagccg ccgaggatgc ccctttttcc
     4561 tggctctcat tttgctcatt aatatcatca cgcagtgctt tgaactgcaa atgcgctcta
     4621 tattcaccat cagcaacccc ggcagggcgc cgaagcgcca gcctgatttt ctgctttgca
     4681 ctcggtggca acgtgacacg gcgaggagta aaaacaacgt attgcgataa atcaaactcc
     4741 gtggctgggc tttccgtaat ggttgttgcc gccccggctc cggtgccgtc gtcctcattc
     4801 atcctgtaat gctgccagcc tatatcgtag gtttttgcct cgtttgaagt gttcacaaga
     4861 gttacctctt taaagcggtc cccttcttca aatacaattc ttgtgggagt gatcgtgata
     4921 tccgccaagg aggttttatg tgcaaacagc gatgcgaaca ttatactgca cacgaacaaa
     4981 accttaagta ttttcattat tctctcctga ttctttttaa aaattaatct gaatttccag
     5041 catgccatta tatgtccgat caagataagg aaccccgttg ccgctggtat ctgcagttgc
     5101 ccccacccgt atctgtgcca cgcctgctcc gtctgttgat gcaggatggc tctccgtaaa
     5161 tgtattcatt tgaaagctta aaccgccgga tgtgaggggg gtggtttgac ttataaccac
     5221 gctggtaata gccgtactgg caggcaatcc atcaatatca taaatcccgt cttgcccggt
     5281 tgccgcaatc tctataaagc ctgccgggtc gtaagtgacc acaccaccaa cattcactgt
     5341 tatcgaatat accgcatcat tatgcgttac aatgtattcg ccgaactgca acgcttgaat
     5401 ttccgtaacg ccagcgaatg cgcgaggtga taaaccaagc aatattaaac ccgctaaaac
     5461 atattgaata tgccgaatca tctcacgcct tttgttgaaa tttgagatca gagtactgaa
     5521 aatcccctta tatcacaagg tagatttttc atcaaaaaaa gcaaaaaacc gcgggtttgt
     5581 gcataaatat gtgcataaaa actgaatagt tgcttgaccg tgagtcatgg ctcacatacc
     5641 cttaaacgtg tatcgttctg atacttttgt gttttctttg ggctgacttt ttacatttcg
     5701 ggcatcgctc cactttttcc tcggggcatt ttggttcatt accatatgaa taagagattt
     5761 ggtttatggg tattttggat gctgtttgcg ggtttgaccg caagctgtgg tcctcttacc
     5821 gatactccca agcccgaaca gaccctgaat tcagcccctt cttcagaaaa ccttatcagc
     5881 ccaaacgccc cgtcacaggc ctcttccact tcagccctgg aagttcccgc ccctattgcg
     5941 atgccaccaa gttatggtga tcgcccgcct gaaccggggc aaatgcaatc cagcactatg
     6001 ccaaacggct tgcctgccct tcagcccaag ggaatcaacg ttgagagcct gtttgcaacc
     6061 aagatcagtg attctgatca gcgctttgcg cggcttgaaa atgccgtgct ggatctgcgg
     6121 cgtgagtttg aaaccttcaa acccgctatc acccgtctgg ttgcggttga atccgatatt
     6181 caggatttga tcaaacaact cgacatctta ctgcgtaatg aaccggtggc gccgcccatg
     6241 ccactaaaac ctgaaaccgg ccctatgccg cttacagcgg cagatcaggc gccgccgccc
     6301 aatgccggag ccattagccc cgctcctcca cagtcgcccg cagcgccccc tgcccccaaa
     6361 gcggtgagtg gcaagccagt tgtaaaagca ctgcgcgttg gacagcatag cgataaaatc
     6421 cgcctagttt tggatgtttc gaagaaaact ccgtatagcg cggatttgga tactgaagaa
     6481 aaattactga ttatcgaact gcccgaagca caatggaatg cagcccgcca gcagagtttt
     6541 gcccgctctg agctgttgca gtcctatagc gttgaaccga ttaatgatgg cgcgggaagc
     6601 cgcgttgtta tgacacttaa acgcccaacg cagcttataa aacagcaagc cctgccgcct
     6661 ggaaatggaa atgcagatca cagaatattt ctggatttga gcttatagtt tttttaaaag
     6721 ggctttacgt tggcccgacg cccaaagatt tgtaaagacg aataagggcc aaagccttag
     6781 cggcttgcgc attggtcagt gcgctttgtg cgtcgttcac ggtacgctga gcgtctaaaa
     6841 catccaggaa tgaaatttcg ccttccttga aaagctgttg ggagagaaaa aatgcttctt
     6901 gtgcattttc gtatgcttta aaaagcgaga aggcctgttc attaatgtgc gcataatcgc
     6961 tgagcgcggt ttcgacttcg acaactgctt cgagaacaac cttgcgatat gattcataag
     7021 cctccatttc gcgggcgcgg gcagcatcaa tttcgccttc cagacggcca aagctcagca
     7081 cggttacggc agcaccgacg gcgacattcc agatgccggt ggaactggcg aaggcatttt
     7141 cagcgacgcc ataaaagccg cttaaggtaa aacttggaaa aatagctgtg agcgttgccg
     7201 cgctcagatt tgtttttgca cgcaaattcg ccgccgccgc gcgaatatca ggacgcagcg
     7261 ccagaacccg cgccggggcc atcagcacag gcgcgacatc gctatccggg atttttttgc
     7321 ttacaaattc tatccctgca agatcatttg gaagtgcacc agtcaggacg ctcaagcgca
     7381 aacgcgcgtt ttcactctgg cgttgaaatt cgggaataga ggccctggtc gtgttgacga
     7441 ggttctttgt acgttcgacg tcaagctgtg gcgattcacc ggcggcgtag agctggtcaa
     7501 caaggctgag cgtttgttcc tgcgttttaa gattgtcttg tgcaatgcgc acttgttttt
     7561 ctgcggcgca catttcggta taggtgcgcg caacttctgc taccaggctg agcgttacat
     7621 cctgatattt tgcctccaga ttttcaagtg tcgcattgga agcagcaaaa cgtttgcggt
     7681 tttttccaaa aatatcaagc tcgtatgata catcaaagcg cgcatcgtaa taatcatccg
     7741 catcgctctg aacggtgtct tccctgccct tggttgcgga tgctccaatt tgcggaaaga
     7801 gtgatgcgcg ggttgccttg cgcaggccgc gggcttcaag aatgcgagcc tcagcaattt
     7861 ttcgatcagg actgtcggcc agagctttat cgatcaacac acttaagagc gggtcgttaa
     7921 aacgatacca ccagttttgc agtgatacgg catttgcacc ggcaatacgc tgttcttgtc
     7981 cgcttgcact gacccagcta cccgggcctt taaagtcagg caaatccaga gaactgacga
     8041 tacaaccgga cagcgttaag gcggagcatg tcaacaaaaa tgatattctc atattttatt
     8101 gtctcctgca gattcaagcg aggccttatg aatgttatga cctgtgagca agacttcgac
     8161 tttacgtata acaacgtaga aaagcggcgt aaaaacaatt ccaaaaaatg ttacgccgag
     8221 catgccggaa aaaaccgcga tcccgatggc ctggcgcatt tccgcgcctg cgccgtggct
     8281 ggtgaccaat ggcaacacgc ccatgataaa agaaaaagag gtcatcagga tcggacgaag
     8341 gcgcatgcgc gcggcctcaa tggcggcttc aacaatcttg cgaccctgca gttccaaatc
     8401 acgggcaaat tcaacgatca gaatggcgtt tttacacgcc agcccggcaa gcacgaacag
     8461 gctgatctgc gtaaagatat tgttagccat acccgtaggc gcgcccatgg acatttgaaa
     8521 ctggccaaat aaatacaccc cgaccatggc cgagagtgtg ctcatcggca cgattaaaat
     8581 cactgccacg ggcaagaaaa gattttcata ctgcgcggcc agtactaaat aggccaataa
     8641 caggcataaa gggaaaatca gcatcgcagt gttacccgcc agaatttgct gataggtcag
     8701 gccggtccat tcaaagctca tgcctggtgg cagggtttcg gcaaggattt tggcaatcgc
     8761 gtcctgcccc tgccctgtgg aatagcccgg cgcaatattg ccattcaaat cggcggagcg
     8821 gaatgcgttg taacgctggg cggcttcggg accgaaggat tgctcgactt tgatgactga
     8881 gcctaacggc accatatcac catttttgtt gcgtgttttc aggcgcagga tgtcggaagg
     8941 atccgagcga tattctttat cgccttgtgc gatgacctga taggtgcggc caaacatatt
     9001 gaaatcgttc acatagagag aaccgagata gacttgcatc gcggcgaaaa catcctggat
     9061 aggaatgccg agttgctggg cgcgctcacg gtcaagattt gcaaaaagct gcgggtaatt
     9121 cacatcatag ttactaaaaa cgtagctgag ggccgggtct tggttggcgc gcatagtgac
     9181 ttgctgaacg gccttgttca gcgcttcgat tcccagatca gcgcggtctt gaatgtgcaa
     9241 ggcaaagcct ccgactgtgc ccataccgcg cacagcaggc ggcgggaaaa ttgccacaaa
     9301 cgcatcgggg atggccatga atttttgctg gagctgcccg gcgattgcgg ggccgtattg
     9361 ctccgggctc atgcggtctt caaaatcatc aagcgtgatg aagacgatgc cggagctagc
     9421 cgagctgata aagccgttga ttgacaggcc ggggaattgt accgcattgc tcacgcccgg
     9481 atgagcagtg gctatatcgg ccatttggcg gataacgctt tcggtgcgtt caaggttggc
     9541 gccgggaggc agcttcgcaa atgccaccag atattgttta tcctgcattg gaataaaacc
     9601 tttgggcacg aggttaaagc tgaatacggt tgcggcaata aagccagcgt agaggatcag
     9661 catgatcacg cgatggcgag tgaagaagcc aacagctttg ctgtagccat gcgcgctggc
     9721 tgtgaaaatt ttattgaaga ttacaaaaaa ccagccaaaa agcacattaa ttaaacgcgt
     9781 caggagatct ttgggcgcgt ctttggtttt taacaacatc gcagaatatg ccggtgagag
     9841 tgtcagcgca acgattgtcg agatgatggt ggcgatggcg atcgtcacgg cgaactggcg
     9901 atagaactga ccggtcagac cgctgaggaa agcaatgggt acgaacacac tgaccagcac
     9961 cagcgaagta gcaatgaccg gcccggtgac ttccttcata gcctggattg tggcatcgcg
    10021 gggtttcaga ccttcgctga tattgcgctc aacattttca actactacga tggcatcgtc
    10081 gacaacaatt ccgatggcca gaataagacc gaagagtgag agcacattga tggaaaatcc
    10141 cagaaaaagc ataatggcga aggtgccgac aatcgaaacc ggcacagcca aaaacggaat
    10201 gaccgaagcg cgccaggtct gcaggaagac aatcaccacc aagactacga gcagcacggc
    10261 ttcaaacaat gtatggatta ccgattgaat agagtctttt acgaatactg tagggtcata
    10321 gacgatagaa taatcaattt cgtcagggaa attctttttg agatcggcca tggcggcgcg
    10381 aacattatcg gaaatttcca gcgcattggc gtcgggcgcg gcaaaaatcg ggatggcaac
    10441 tgccggctca ttattcaaaa atgatcgcag ggcatagctt tccgcgccaa gttcaagacg
    10501 cgcgacgtct ttgagccgcg tcaacgcgcc gttttcatcg gctttgataa taatattttc
    10561 aaattcctca acgctttgca gacggccttg cgtgttgacc ggtaattcga cctgcaggcc
    10621 ttcttcataa ggcgcaccac cgatcgttcc ggcggcgacc tgtaaattct ggccgcggat
    10681 ggatgcgatc acttccccag gagtcatgcc cagttcagca atggcttcgg ggttgagcca
    10741 gacacgcatg gcataatcac cggagccaaa gaccatcacg cggcccacgc cagagatttt
    10801 ggccagccgg tccttgacgt taatcgtggc gtaattgcgc aaatacagca tgtcatagcg
    10861 ctcggacggg gagcgcaagt gtacgaccat cgttaaatcc ggcgagcttt tggttacggt
    10921 cacgccaagc tggcgcgtga catcgggcag gcgcgggagc gcctgtgaga ctcgattttg
    10981 cacaagctgc tgggccagat cgacatcagt gccgatctcg aacgtgatgg tcagtgacat
    11041 acggccatcg gcagaagcca gcgaactcat atagagcata ttttcaacgc cgttgatttg
    11101 ctcttccagc ggcgtggcca cggtttctgc aatcgtcatc gggttagcgc caggaaaagc
    11161 ggcgctgaca ttgaccgaag gcggggacac ttctggatat tcggaaatcg gcaggttaaa
    11221 catcgcgatg aacccggcga taaagatcag cagcgaaagg acgctggcaa aaattggacg
    11281 atcgatgaaa tagcctgaca gggtcatgtc tttacgggcc ttttatggaa gaggacgacg
    11341 acgtttcatc ctgaggtggt tcttcgcttg gcgccgcagc aggcacgctt tcagctttat
    11401 cctgctcccc tgccatcatc ggcgcaaccg gcattccggg acggatcatt ataatgccct
    11461 gagccacgac tttctcgccc tcggacaggc cggaaaggac aacgcggctg tgaccgctac
    11521 gatcaccgag ttgaacctga cggtattcaa taacattgtc cttatttacg acatagacaa
    11581 agcggcgatc ctgatcgatg ccgatcgctt tttcgccgac gagcatccga cgcgcgcgat
    11641 cggcgctacc caaccgcact ttggcgaaca tgccggataa aagggcgcca tcactatttt
    11701 ccagcaccgc acgggcgcga atagtgccgg aagccggatt gatgcggtta tcaaaagcat
    11761 ggattacgcc atcgattggc tgctcagcgt caccacgcaa aatgacctgc acagggattt
    11821 taccttcgtc ttctttgctg cgcacctgtt cacgaatatt tgaaagatag gtttgttcgt
    11881 caacttcgaa atcaacatag atcgattcat catctacaat cgtcgtgaca atgggcgcac
    11941 ccggcccgct ttcgacgaca ttgccgagcg tgatttcagc gcgactgacc cggcctgaaa
    12001 tgggggcttt cacacgggca tagtccagat caatttcggc cttttgcaag gcggcctttg
    12061 caccgctgat ttcagccaga acaaaattaa agcgggaacg gcgctcatcg acgatgcgct
    12121 gggaaatggc gttggttttg atgagttcct gcgcacgctt atattcttta tcggccagat
    12181 tgtaattgct ttgcgcagcc tgaagagcgg ctttggcctg ctcaaccgcc gctttatagg
    12241 ggcgagaatc aataacgaaa aggacatcac ctttcttaac atgttggccg tcctgaaata
    12301 aaatctcggt gatgcggcca ctgacctgcg ggcgaatggc ggcataatcc actgcgctca
    12361 agcggccagg gaaatctttc cagatgcgaa catcctgtgt gccgataatt tcgacactga
    12421 caggcatcgg gccctgtggc ataccttcgc ccggcccttg tgcatcggca tggttaaacg
    12481 gattgagtgc gataaacagg cccgccacaa acaacatgat tacgcaaaag acggtcagtt
    12541 ttataagcaa ttttgaaagg attaattttt caagaagtga ctttggcatt ggtttgtctt
    12601 tcttgggcct gtggaaaact caggagttaa aataaatgct cactttacaa aaccttgcaa
    12661 tggttatcag agacttaggg ggtttttatg tttggctgag aacaagactt tgtaaagact
    12721 attaacttta ctctataatc gtccaaaaat gaagaggctt aaaacctaca agatctcatt
    12781 ctttgacatt gaaccattcg aaagaacagg cgacgaattc aggattgagc cattttggat
    12841 tcgcaccgcc atatttgagc acttcgccat ttacgtctgt gatcacaccg cctgcggctt
    12901 gcaagaccgc atcagctgca gccgtatccc attcacatgt cgggccgagg cgcgggtaga
    12961 tgtcggcctt gcccgccgcg ataacacaaa ttttcaatga acttccgcgc ttgatcagtt
    13021 ttttgattct aaattgctca aggaattttt ccagtcgctg cgcatcacca tgcgatttgc
    13081 tggcaactac gttcaatccg cctttgggcg gcggacgcac agagatgggc ttttccgtgt
    13141 ctgtgtcttg tgaccagcgg atagcagtac cttcgccatg gcccgcataa agctcaccta
    13201 aggccggagc atatactaca cccagaactg gcttggcatt tttaacaagc gctatattta
    13261 cagtgaattc ctcacctcca ttcataaatt cttttgttcc gtcgagcgga tcaacaatcc
    13321 agaaatattc gtgaccttct accgaggtct ttttaccaag agcaacggcc tcttcgccaa
    13381 tcacaggaat atcgagcgta agctgttcca gtgacatctg gataaaggtt tcagcctctc
    13441 gatcggccaa tgtgacggga gaatcatcgt gcttggaatc aacctctaat ccgtctaaat
    13501 cctcataata tttcattata atatcacccg caccaagtgc gatgcggcga accatattgc
    13561 atagagcctc aggattggcc aaaagctttc tggtgcgctc acgggctctt ttatgaaatt
    13621 cagcgtcgaa cattgagttg ggattaaaag acaatgaatt tttccttatt ttgagacttg
    13681 ttgattttat cttcgggata aacctgctgg ttaatatact tcaaaacttt ctgaatgcag
    13741 gcattaaatt agccttgcat gttaattata tcacgctcag cgaattggag cgctataaac
    13801 ttcataaata atatagatgc tttgtgcctt gagtttgttg cgatattggt acacagacct
    13861 atgatacctt cttgagtttt gcttcattgc cgggcggcac ttccgggcgg ccaatcgaga
    13921 catagtggaa gccgcgctcg cgcatatctt cgggtttgta gatattgcgc aggtcgatca
    13981 tgcgtttttc tttcatgatt tcgcccaaac gccccagccc aagtgcgcgg aactcgttcc
    14041 attccgtaac gatcaccagt gcatccgcat tggtggccgc aacgtaggcg tctttacacc
    14101 attcaacctt gctcagatgc tttttcgctt cgtccatagc gacaggatca aacgcggcca
    14161 catgggcgcc ttcgtcctgc agatagggga tgatctgcaa ggatggcgcg tcacgcatat
    14221 cgtcggtgtt gggtttaaaa gacacaccca aaatagcaat gcgcttgcct tgaacgttgc
    14281 ctccacaagc ttcgatgacg cgctgggcca ttgagcgctg gcgggcagta ttgacccaca
    14341 ccacggtttc tacaatattt tgcggcgcgt tgtatttctg gccgatttgt gtgagggcga
    14401 gcgtatcttt cgggaaacag gagccgccat agcccggccc tgcgtgcagg aacttcgcac
    14461 ctatacgccc gtcaaggcct attccacgcg cgacatcctg tacattagca ccggtttttt
    14521 cgcacaaatt ggcgatttca ttgataaagg taatttttgt ggccaaaaag gcgttggccg
    14581 catattttat ggtttccgag gtttccggcg atgttatcac catcggcgtt tcgttaatat
    14641 aaagcgggcg gtacagatcg cgcataattt caacggcgcg ttcgctatcg gcaccgatca
    14701 cgacacggtc aggacgtaag aaatcattca ccgccgcgcc ttcacgcaga aattcagggt
    14761 ttgagcagac atcaaactcc acatctgtgc gaccctgttt tcccaattcc tcattcatga
    14821 tgcgcttaac ttcgcgcgcg gtgccgacgg gaacggtcga tttggtcacg atcagcgtat
    14881 agccttccat cacacgggca atttcgcgcg ccgcctcata cacatatgtc atatcggcat
    14941 gaccgttatc actgcgcgct ggcgtaccca cagcgataaa aacggctatc gagtctttga
    15001 gcgcttcttc aagatctgtc gtaaaggata gacgcccggc ctttacctgc ttggcgacaa
    15061 ggtcctctag acccggttca tagataggga tttcgccttg cttaaggcgc ttaatcttgc
    15121 cttcgtcctt gtccagacac acaacgtcga tcccgaattc agcaaagcat gtgcccgaaa
    15181 ccagcccgac atagcctgtc cctaccattc ctatacgcat gattatccct ttcaatcaga
    15241 gcgttaaatg taaatctttt gcgccttttt cgcactgctc agaccttttg acaagcttta
    15301 ttccataccc agaaagaagt aaccagttgt taagataata cggctaagct tattagagtg
    15361 atcaggttta cctttcaact tcacatattt tttatctagg gtcaggacct aaaacattgc
    15421 ataatctttc atattttgtt taaattcaac ttctgaaatt taattccata gaattttaaa
    15481 ggcatttcaa acgataagga tcaataatgg ccaagaaaga gacaaaaaaa gacgcaccag
    15541 ccaaggcaga gccaaaagcg tcagagcaaa agccagcagc caatatgccc ttgttttatt
    15601 ccaaccctgt gccgcttgat gccaaggcgc atgcggactt ggctttgaaa cagaattttg
    15661 gtttcggctt tacagaaggt gtgaatgctg tgccgcttaa cctgatcgaa atgccgcaag
    15721 tttgtcatta ttacccgatt gcattttcgc ccgacggcaa cgcaacgcct gtg
//
LOCUS       DAOSWR010000038        15313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2427_length_15313_cov_5.713789, whole genome shotgun sequence.
ACCESSION   DAOSWR010000038 DAOSWR010000000
VERSION     DAOSWR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2427_length_15313_cov_5.713789"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /locus_tag="PK513_04375"
     CDS             <1..1970
                     /locus_tag="PK513_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO81717.1"
                     /translation="QVDLSKYTPDMFAKQNIFSRPSASDKPSADKEPARIAITEDGRI
                     VYASTAFSELSGTKKETLSGRTASTIINFSETSQTSPDFKTIEPGLHKIFIKGHANSF
                     DFHFDWLTTPDNKRFLIGSQTSENKKPSKTQMKNFIRRLKEIETAPEISAALSPQETE
                     NFLNLSHEVMLIVTDLGEITKANQTFCDLTGYNLKDLHAIPFIELFDEQDRSYVRSTM
                     QAFNPAGYEFALGAAQNFEARIVTKDRQCLWMEWRQQFENGTLYCTGRDITAIKQQEK
                     ALLRREKQLLQAESIGHMGHWNWVIGEETMDWSDEIYRIFGVERTGFSPTLEHMDDMV
                     HREDIARVNQAFQRAIIEENDYDMEFRINRPDDTIRFVRCEGRCAFDDKGEVIALYGI
                     MQDMTERTLYERDLRKAKEQAEAAYASRTQFLANMSHELRTPLNAIIGFSEMIERQLL
                     GPIGTEKYLEYISGIRESGQHLLDLISDILDMSKIEAGKYELTLEEISIAKIIQLCGH
                     MMEGRAHESGVKITLDHTLDEDFKIIADRRAILQILLNLLSNAVKFTPEGGEITLTGL
                     QRENYLSLKIADNGIGIPANKLASVTKPFEQVSSSYARNHEGSGLGLAISKELAEMHG
                     GTLFIESAVGQGTTVTVRIPFDASKTLKNSA"
     gene            complement(1981..2418)
                     /locus_tag="PK513_04380"
     CDS             complement(1981..2418)
                     /locus_tag="PK513_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81718.1"
                     /translation="MATRVERVCGSKEECSVPATTSEPQKSPSGLYELQIVEGRDDAH
                     YLQFQVLSTTRQSDSEIYKTVETVLVPEEKFYTRHRTYIMWDDEEDRVWVYSGDVGIF
                     FWDMAASGQWEKQICIPRGENVRDVPIFLKEVLREQFHSCFYD"
     gene            complement(2735..3664)
                     /locus_tag="PK513_04385"
     CDS             complement(2735..3664)
                     /locus_tag="PK513_04385"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO81719.1"
                     /translation="MSLASIPYRLFCFGYGYCCDYLGYDLVGREDWQVAGTTRDAEKR
                     DILKERGVEAYIFDKDVPLPDPASLLENTTHLLISTPPDSEGDPVFQAHAQDILSLPN
                     LQWVGYLSTTGVYGDRGGEWVNEASQRQPSSQRGSRREKAEDQWLSLFKNYGLPLHIF
                     RLAGIYGPGRSALDSIRAGRARRIDKPGHAFSRIHVEDIVRVLQASMQRPNPGAIYNV
                     ADDEAAPSHKVIEHACKLLRRPVPELEMFDEADLSPMARSFYYDNKRVKNERIKNELG
                     VVLKYKTYREGLQACLDAEEYALSVFKSNGKLF"
     gene            complement(3707..4375)
                     /locus_tag="PK513_04390"
     CDS             complement(3707..4375)
                     /locus_tag="PK513_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOO81720.1"
                     /translation="MRTLYHLWLHPFSRKVRIVLAEKKLDFTLQIEKVWERRTDFLAL
                     NPAGDVPVLIEKDGTTLANSQVICEYLEEVYPEVNLLGHDPVQRSEARRLVSWFDVKF
                     NREVTDNIVGEKIMKRLLKVGEPHGPAIRAGHANIHYHLDYIGFLTEKREWLAGDKFS
                     LADIAAAAHLSAVDYIGDVPWEEHRAARVWYEKITARESFQPLLEERIPGFASVGDLA
                     AVGT"
     gene            complement(4480..5736)
                     /locus_tag="PK513_04395"
     CDS             complement(4480..5736)
                     /locus_tag="PK513_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOO81721.1"
                     /translation="MQVHEIFMMKTVSQDYNRLARLFHWGMALLIFGLLIVGFVMTDL
                     PASPMKFQVYGLHKSMGLLVLGFGFMRLIWRFMSAYPLSLSTHVRWERFLSKTIHIVF
                     YVSIFVMPLSGWVMSSAGAHPVRFFGLFEMPALGSKNEVVFEAMEAVHLTFAVVLVGA
                     LGLHVAGALKHHLIDRDETVRRMGGNRTFAIIGVLLLMASSLLAGDEILDLVSAESEN
                     GHNAVNDSRDSFHELRVEVVKNNQDGESVSEWVIDPMESVIALSFLQYGQSVDAAFMN
                     WEAQIVFDPQGLDHSFVRAEIDMTSLETGSMERDVQVRGEEWFDVQTYPRAIFESESF
                     ESLGANRYRADGVLSLRGVKNQLSFPFSLTITPLDGGAKQATMAGDFTLNRLDLGIGQ
                     GTWKSTEAIGNSVNVRVNIVARASGI"
     gene            complement(5761..6369)
                     /locus_tag="PK513_04400"
     CDS             complement(5761..6369)
                     /locus_tag="PK513_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOO81722.1"
                     /translation="MSRLSLSVVAAALLFVPASVQAQVEKYSFDKAHTQILFFVNHLG
                     FSNSQGEFHDYDGSFTFDQTHPENSSVDVTIQSASIDMDDEKWDEHLKTADFFAVEKF
                     PTMHFKSTDIEITGDNSANITGDLTLLGVTHPVTLAVVHNKSDKHPFSGKYVSGFSGS
                     AILKRSDFGMNYGIPGVADEVQIRLEVEGIREEQEGEGTNNP"
     gene            complement(6429..6647)
                     /locus_tag="PK513_04405"
     CDS             complement(6429..6647)
                     /locus_tag="PK513_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81723.1"
                     /translation="MIYVIGIVGFIGGFIFGQMVLYFLLRHKTNQELLEDRMLKIKYG
                     LIGWGCAALGAYSFVEIYKVYFPSAVLS"
     gene            6698..7516
                     /locus_tag="PK513_04410"
     CDS             6698..7516
                     /locus_tag="PK513_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOO81724.1"
                     /translation="MTVLPLIILALVQGITEFLPISSSGHLILVHRIFGDIESWENRL
                     VLDVAVHVGTLFSVLIYFRKDVIAMLCGLINTLRGNFTHRGARLNLHLIIASLPIIIA
                     GLIVQILQPGWLLLLNVIALTTLIFGILLWIADRKPAEGKTLENLNFKDAFLIGCAQT
                     LALIPGTSRSGITMTAARFLGYSRTESAHFSLLLAIIAITGAGTLASLELYQSGDITL
                     GLDALLATILAFIAGWASIVLMMKWLERASFTIFAIYRIILGAVLLIILNTGIF"
     gene            complement(7513..8070)
                     /gene="rsmD"
                     /locus_tag="PK513_04415"
     CDS             complement(7513..8070)
                     /gene="rsmD"
                     /locus_tag="PK513_04415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOO81725.1"
                     /translation="MRIVAGKYGGRRLEVPGGRDIRPTSDKVRGAVFNALESMGAVRG
                     ARVLDCFCGTGALGLEALSRGAEFCSFMDKDKRSLALTRSNAERLDAALQSEFFLRDA
                     QRFIRPSSMPKYSLVFLDPPYRKGLIDIVLQALEEGECFDTAAVIVLESEKGYAPNMS
                     GSFTVQSTKTYGDTQVQFLNYTGQL"
     gene            complement(8070..8822)
                     /locus_tag="PK513_04420"
     CDS             complement(8070..8822)
                     /locus_tag="PK513_04420"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009814307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO81726.1"
                     /translation="MSEENHKGERIAKVMARAGLCSRREAECWIEAGRVQVNGERLAS
                     AACVVGVDDRIEVDGRLLGLAEEARLFLYHKPSGLVSTHKDERGRKTVFDMLPKELPR
                     VMSVGRLDLNTEGLLLLTNDGGLSRYLELPATGWSRRYRVRVLGAVDEARLADLKGGI
                     TVEGVRYKSIEAKLEQTGKSGANRWISVTLREGKNREIRRVMGALGLQVNRLIRVGYG
                     PFELNKLPCGGVQEVEAHVMKAQIPGYFKGHV"
     gene            8821..9291
                     /locus_tag="PK513_04425"
     CDS             8821..9291
                     /locus_tag="PK513_04425"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOO81727.1"
                     /translation="MTSHTSDQDHIYMRAALEEAKTAEALDEVPIGAVLVSPETGEII
                     AAHGNRTRTMCDPTAHAEILCIREICSFEKAQRTPGYDLYITLEPCAMCAAAISFARL
                     NRVIFGAHDPKGGGILHGGKFYDQPTCHHRPAVTHGILADECSQILRDFFKAKR"
     gene            complement(9292..10584)
                     /gene="purD"
                     /locus_tag="PK513_04430"
     CDS             complement(9292..10584)
                     /gene="purD"
                     /locus_tag="PK513_04430"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO81728.1"
                     /translation="MKVLLVGSGGREHALAWALAASEKCNALYCAPGNAGIEACAECV
                     GIGAEDVQGLVGFAKDKAIDLVVIGPEAPLVAGLADGLAAVNIAVFGPDKKAAQLEGS
                     KGFMKDLCAKYDIPTASYGRFTDIEAAQQFIEDQPGNVVVKADGLAAGKGVIMCDNKA
                     EAVEAAREMLSGGSFGEAGTEIVIEEFMDGEELSYFALADGKSVLPLSSAQDHKRAYD
                     GDEGPNTGGMGAYSPAHLMDDALEQKILERIIQPTIDGMAAEGCPFTGVLYAGIMMVD
                     GEPKLLEYNVRFGDPECQPLMMRLQGDLLEILDAAAHGRLAEIADRVSWSDKSALCVV
                     MATKGYPGSYPKNTVIKGLEDTAEVKDVTVFHAGTAKDENGQIISTGGRVLGVTALGE
                     TIAEAQRRAYMGVNKINWPDGFCRKDIGWRALKAEEAA"
     gene            complement(10627..13707)
                     /gene="putA"
                     /locus_tag="PK513_04435"
     CDS             complement(10627..13707)
                     /gene="putA"
                     /locus_tag="PK513_04435"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HOO81729.1"
                     /translation="MTSPSFSPYFQRSEEAAVKGLLQDFDWDEGRAARIGKEAAALIE
                     AMRGQKQEVGQLESFMRDYALDTEEGLALMMLAEALLRIPDKATRRELIRDKVAAANW
                     LEGVGGSKDWVVKAAGVGLFMTSRTLNGALSKIGEPIVREAMLKAMRMLGGQFVLGQD
                     IEEAMQKALPLRNRGYRMSYDMLGEGARSAADAQHYYEAYESAIEYIGQRADKKLVRR
                     PGVSVKLSALHPRYEFAQSEICVPEMAARLLALLQKAASYDLALTVDAEECARLNLSI
                     EIIERVLGDASLGDWDGFGLAVQAYHKAAPAVIDHVAEMAKARTRRIQMRLVKGAYWD
                     SEIKKAQVAGLCDFPVYTRKSHSDTSYLVCAHKMFDYVDTLYPMFGTHNAYSVAAIMD
                     MAHNVGAGAFEFQRLFGMGGALFDQLLDRGAVASIYAPVGPHKDLLPYLVRRLLENGA
                     NSSFVNRGLDPDASVESLVVDPVDKSKNRKEFKHPKIVLPKNLFMDETPQGRANSSGV
                     DLDAPETLAQLSQEIGAYDNSSVSAAALIDGRTLKSGAARTALNPADRADVVGQVYDA
                     DFKTVERAFEAAQAGFVEWNACDSDVRARALECAADMLEERRGEFMALLVREAGKTLV
                     DAVSEIREAVDFLRYYASRGRKLFAAEGEALPGPTGERNRLYQQGRGVFVCISPWNFP
                     LAIFTGQVAAALMTGNAVIAKPAEQTPLIAYKMVELLHEAGVIPAALHLLPGDGRVGA
                     CAVEHGLCAGVAFTGSTEVARIINRALAEKNGPIVPLIAETGGQNAMIVDSSALPEQV
                     VDDVILSAFGSAGQRCSALRVLCVQEDIADKVVRMLAGAMDELCIGDPARLSTDVGPV
                     IDEEAMAMLVHHHEALKGFGQCIGKVTVSNALARKGHYFGPCAYEIPGMGALTKEVFG
                     PILHVIRYSKDGLDDLLRDINASGYGLTLGIHSRLESFHQYVAAGVRAGNVYVNRSMI
                     GAVVGSQPFGGSGLSGTGPKAGGPHYLARFASERVISVDTTAAGGNASLVSLSE"
     gene            13755..15212
                     /gene="xseA"
                     /locus_tag="PK513_04440"
     CDS             13755..15212
                     /gene="xseA"
                     /locus_tag="PK513_04440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO81730.1"
                     /translation="MSESEAQKTKIQTETHRSNIPEFSVSDLALSLKRTLEENYGRIR
                     VRGELSRISIAGSGHLYSSLKDDQAVIDAVCWKGVLSNLSVKPEEGLEVICTGRISSY
                     PKSSKYQLIIESMELAGEGALLKMLEERKKKLGEEGLFAPERKKPLPFLPQTIGVVTS
                     PTGAVIRDILHRLADRFPRHVLVWPVLVQGDGAANQVASAIRSFQTIHKHGQPRPDLL
                     IVARGGGSLEDLMPFNEEDVVRAIAESEIPIISAIGHETDTTLADFAADVRAPTPTGA
                     AEMAVPVRANLIAQILEDEQRLISGMTRLLSEHRHKIESLSARLGDPARLLETQTQRI
                     DHLSYKLSGLFSRFLDTKKHALSEQTARLRHPRERLAEASQNLSRWNEHLETLSLKLT
                     REPTQKLDHLGKMLEAYSFENILQRGFSVVRDEKGTLIPDASNIHPNQVLDIQFRNQG
                     HIQAITTSKKASKPKTKKRKAPPPAQNDPQTNLFD"
BASE COUNT         4282 a         4884 c         2881 g         3266 t
ORIGIN      
        1 ggcaggttga cctttctaag tacactcctg atatgtttgc aaaacaaaat atattctctc
       61 gtccctccgc gtccgacaaa cccagcgcag acaaagaacc tgcacgtatc gcaatcaccg
      121 aggatggccg cattgtttac gccagcactg ccttttccga actttccgga acaaaaaaag
      181 aaaccttaag cggacgcacg gctagcacaa tcatcaattt ttcggaaact tcacaaacca
      241 gccccgattt taaaactata gaacccggcc tgcacaaaat ttttatcaaa ggccacgcaa
      301 acagttttga ctttcatttt gactggctta caacgccaga caacaagcgc tttctgatcg
      361 gctctcaaac atcagaaaat aaaaaacctt ccaaaacaca gatgaaaaat ttcatccgcc
      421 ggttaaaaga gattgagact gccccagaaa tttccgccgc actatcaccg caggaaacgg
      481 aaaattttct aaacctctct cacgaagtga tgctcatcgt cacagattta ggcgaaatca
      541 ccaaagccaa ccagactttt tgcgacctga ccggctataa ccttaaagat ttgcacgcca
      601 ttcccttcat cgagcttttc gatgaacaag accgctctta tgtccgcagc acaatgcagg
      661 ctttcaaccc cgcaggctac gagtttgcac taggcgcggc gcaaaatttt gaagcccgga
      721 tcgttaccaa agacaggcaa tgtctctgga tggaatggcg tcaacaattc gaaaacggaa
      781 cgctctattg caccggacga gatattactg cgatcaagca gcaggaaaaa gcgcttctcc
      841 gccgggaaaa gcaactctta caagccgaaa gcattggcca catgggccat tggaactggg
      901 tcatcggcga agaaaccatg gactggtcag acgaaatcta tcgcattttc ggcgtcgaaa
      961 gaaccggctt ttctcccaca cttgaacata tggacgatat ggtccaccgc gaagatattg
     1021 cccgcgttaa ccaggccttc cagcgcgcaa ttatcgaaga aaacgactat gatatggaat
     1081 ttcgaattaa cagacccgat gacacgatcc gcttcgttcg ttgcgaaggc cgctgcgcct
     1141 ttgatgacaa aggcgaagtt atagccctct acggtatcat gcaggatatg accgagcgta
     1201 cgctgtatga acgcgacttg cgtaaagcca aagaacaagc cgaagccgcc tatgcttcac
     1261 gtacacaatt cctcgccaat atgagccatg aattgcgcac acccttaaac gccattatcg
     1321 gcttttcaga aatgatagaa cgacaactac ttggccccat cggcaccgaa aaatacctcg
     1381 aatatatttc cggtatccgg gaaagcgggc aacacctgct cgatctcatc agcgacatac
     1441 tggacatgtc caaaatcgaa gccggaaagt atgaactcac tctcgaagaa atcagcatcg
     1501 ctaaaatcat acaactttgc ggccatatga tggaaggccg tgcccatgaa tccggggtaa
     1561 aaatcacact cgaccacacc ctggacgaag acttcaaaat tatcgcagac cggcgtgcca
     1621 ttttacaaat cttgctcaac ctgctttcca acgctgttaa attcaccccc gaaggcggag
     1681 agattaccct tacaggatta cagcgggaaa attatctgtc tcttaaaatc gccgacaacg
     1741 gcatcggcat tcccgccaat aaactggcca gcgtcacaaa acctttcgaa caggtctcct
     1801 cgtcctacgc ccgaaatcat gaaggctccg gccttgggct ggccatcagc aaagaactcg
     1861 cagaaatgca cggaggaaca ttattcatcg aatcggccgt gggacaaggc acaaccgtaa
     1921 ccgtacgcat ccccttcgat gcttctaaaa ccctaaaaaa cagcgcttaa attaacgatc
     1981 ttaatcgtaa aaacagctat gaaattgctc acgaagaact tccttgagga aaatgggtac
     2041 atctctaaca ttttctccac gtggtataca tatctgcttt tcccactgcc cggaggcagc
     2101 catgtcccaa aagaaaattc caacatcgcc gctatacacc cacacacggt cctcttcatc
     2161 atcccacatg atgtatgtac gatgccgtgt atagaatttt tcttccggca ccaacacagt
     2221 ctctaccgtt ttatatattt cactgtcact ttgtcgtgtt gtggaaagca cctgaaattg
     2281 tagataatgc gcatcatccc gcccttctac aatttgcaac tcatacagtc ccgacggaga
     2341 cttttgtggt tcggatgtcg tggccggtac ggaacattcc tcttttgaac cgcaaacacg
     2401 ctctaccctt gttgccattt caaacaacat tgccgatccc acagaaaaaa taataaaaac
     2461 gccgacaatg aacacaagca attttgaacc aaattttccg tacaaccacc ccaacacact
     2521 gccaagaaac gctgtcggaa cgacataaac aggaaaagag acaataaaag cgataaaaaa
     2581 cgacaccctt ccatcaggag aatcttgtga aaaccatgaa gcaaaagcgc ttagaatgag
     2641 tacaaaaatt attgatacga caaacccgaa ggcaccgcct ttctgccaac ttttcagttc
     2701 tttgaatcgc ctatgcactg atcatcgctc tgcattaaaa taattttcca ttcgacttaa
     2761 acaccgacag agcatattcc tcagcatcta gacacgcctg aagaccttcc ctgtacgtct
     2821 tgtatttaag caccacaccc agctcatttt ttatccgctc attcttcaca cgcttattat
     2881 catagtaaaa gctccgcgcc ataggcgata aatccgcctc atcaaacatc tcaagctccg
     2941 gcaccggacg ccgcaataac ttacaggcat gttcaatgac cttgtgactg ggcgccgcct
     3001 catcatccgc aacattataa atagcgcccg gattaggacg ctgcatagag gcctgcaaaa
     3061 cacgtacaat atcttctacg tgaatacggc taaaagcatg cccgggctta tcaatccgcc
     3121 gcgcccgccc cgcacgaatg gaatccagcg cactgcggcc cggcccgtaa atcccggcca
     3181 aacgaaaaat atgcagtggt aaaccataat tcttaaacaa cgatagccac tgatcttccg
     3241 ccttttcccg ccgtgaaccg cgctgcgagc taggttggcg ctgcgaagct tcattaaccc
     3301 attcaccgcc ccgatccccg taaacaccag tcgttgagag atagccgacc cactgtaaat
     3361 tcggcaaact caaaatatcc tgcgcatgcg cctgaaaaac ggggtcccct tcactatcgg
     3421 gcggtgtaga aatcaacaaa tgcgtggtat tttccaaaag agaagccgga tcaggcaaag
     3481 gcacatcctt atcaaaaata tatgcctcaa cgccacgctc tttcaaaata tcacgcttct
     3541 ccgcatcgcg agtggtaccg gccacttgcc aatcttcacg cccaaccaaa tcatacccca
     3601 aataatcaca gcaatagccg taaccaaaac aaaacagccg atagggtata gacgctaaag
     3661 acaaaaaagc ctcatcacat tcgttaaaaa ttaaaggact ggtgagctat gttccaacgg
     3721 ccgccaggtc gccaaccgac gcaaatccag gaatacgctc ttccaaaagc ggttggaaac
     3781 tctcacgcgc cgtaattttc tcataccaga ctcgcgccgc ccggtgctct tcccatggca
     3841 catcgccaat ataatcaacc gccgaaagat gagccgctgc cgcaatatcg gccaacgaaa
     3901 atttatcacc ggccagccac tcccgctttt cggtcaaaaa cccgatataa tcgagatgat
     3961 aatggatatt ggcatgcccg gcgcgaatcg cagggccatg cggctcccca accttcaaaa
     4021 gccgcttcat aatcttttcg ccaacgatat tatccgtaac ctcacgatta aatttcacat
     4081 caaaccagct caccagcctg cgtgcttcag aacgctgcac cggatcatgg ccaagcaagt
     4141 tcacttccgg ataaacctct tcgagatatt cacaaatcac ttgcgaattg gccaaggttg
     4201 ttccgtcctt ttcaatcaac accggcacat cacccgcagg gttcagagcc aaaaagtccg
     4261 tgcgccgctc ccagaccttt tcaatctgaa gtgtgaagtc gagcttcttc tcggccagaa
     4321 cgatccgaac cttgcgcgaa aaaggatgca accacaaatg atacaatgtt ctcataggga
     4381 aaatcttagc gcagtttttc gaatcagtgc aatcgccttt acacaggaaa atacaaaata
     4441 aaaaccctgg tttttaaaaa ataagcgcct tccgcacact caaattcccg acgctctggc
     4501 tacaatattg acccgtacat tcaccgaatt tcctatagct tccgtgcttt tccatgttcc
     4561 ctgaccaatc cctaaatcca gccggttaag cgtaaaatca cccgccattg tcgcctgttt
     4621 agctccacca tcaagcgggg taattgttaa agaaaaagga aaagacagct ggtttttcac
     4681 tccacgcaag ctcaaaacac catcggcacg ataccgattc gcccccagac tttcaaatga
     4741 ttcgctttca aaaatcgccc gcggataagt ctgtacatca aaccactcct ccccgcgcac
     4801 ctgcacatca cgctccatgc tacccgtttc caaactggtc atatcaattt cagcgcgaac
     4861 aaaactatga tcaagcccct gtggatcaaa cacaatctgt gcctcccaat tcataaacgc
     4921 tgcatcaacg ctctggccat attgtaaaaa tgataacgca atgacactct ccatcgggtc
     4981 aatgacccat tctgacacac tctctccgtc ctgattattt ttgaccactt ccacccgcag
     5041 ctcatgaaac gagtcacgcg agtcattgac tgcattatga ccattctcag actctgcgct
     5101 caccaaatct aagatctcat cccccgccaa caaactggac gccatcaaca acaaaacacc
     5161 aattattgca aacgtacggt ttccacccat ccgacgaacc gtttcatccc ggtcaatcag
     5221 atgatgtttc agcgctccag ccacatgcag gcccaacgca ccaacaagaa ccaccgcaaa
     5281 agtcaaatga accgcctcca tagcttcaaa aacaacctca tttttagagc caagcgccgg
     5341 catttcaaac aaaccaaaaa aacgaacagg atgcgcccct gctgaagaca taacccaacc
     5401 tgaaagcggc ataacaaaga tcgatacata gaaaacgata tgtatcgttt ttgacaaaaa
     5461 ccgctcccag cgcacatgag tagacaacga caaaggatat gcgctcatga accgccaaat
     5521 caaacgcatg aacccaaagc ccaaaacaag caatcccata gatttatgca aaccataaac
     5581 ctgaaacttc attggactgg caggtaaatc agtcatcaca aacccaacaa tcaacaaccc
     5641 gaaaatcaac agcgccatcc cccaatggaa caaacgtgca agccgattat agtcctgaga
     5701 aacagttttc atcataaata tttcatgcac ctgcatgcct ctttatcaat cgtaaaacat
     5761 ctacgggtta ttcgtccctt cgccctcttg ctcctcgcga ataccttcaa cttccaaacg
     5821 aatctgaacc tcatctgcaa cgcccggaat accgtaattc attccaaaat cagaacgctt
     5881 caaaatcgcg ctccccgaaa aaccggaaac atatttaccg ctaaacggat gcttgtccga
     5941 tttattatgc accaccgcca gcgtcaccgg atgagtaacg cccaggagcg tcaaatcacc
     6001 ggtaatattg gcgctattat ccccggtaat ctcaatgtct gtacttttga aatgcatcgt
     6061 cggaaacttc tcaaccgcga agaaatcagc cgtttttagg tgctcatccc acttctcatc
     6121 atccatatcg atactggcgc tctggattgt tacatcaaca ctcgaatttt caggatgcgt
     6181 ctgatcaaaa gtaaaagacc cgtcataatc gtgaaactcg ccctgcgaat tcgaaaaccc
     6241 gagatgattg acgaaaaaca aaatctgcgt atgcgcctta tcaaaactat atttttcaac
     6301 ctgcgcctgc acagacgcag gaacgaacaa caaagccgcc gctacaaccg ataacgataa
     6361 acgtgacata atctctctcc taaaaaataa aagtaattgt acaaaaaata gatgcaccgc
     6421 ctgtaaaatc aagacaggac ggcagacgga aaataaactt tataaatctc gacaaaggaa
     6481 tatgctccga gcgccgcaca cccccagcca atcaatccat attttatctt caacatacga
     6541 tcttctaaaa gctcttggtt ggttttatga cgaagcaaaa aatacaacac catctgcccg
     6601 aatataaatc cgccaataaa cccaacaatg ccaatcacat aaatcatacc cactattttg
     6661 ctttaactgc agcaaaaatc aagttaagcc tatagccatg acagtgcttc ctttaatcat
     6721 tctcgccctc gtgcaaggca taaccgaatt cctgcccatc agctccagcg gccacctcat
     6781 cctggtccac aggatattcg gcgacattga aagctgggaa aaccggctag ttctagacgt
     6841 tgccgtacat gtcggcaccc tgttttcagt cctcatctat tttcgtaaag acgttatcgc
     6901 catgctctgt ggccttataa acaccctgcg cggaaatttc acgcatagag gcgcccgact
     6961 caacctccac ctgattatcg cctcactccc gattatcatc gcaggcctta tcgtacaaat
     7021 cctccaaccc ggctggctcc tgctactcaa tgtgatcgcg ctaacaaccc tcatcttcgg
     7081 catcttgcta tggattgcag atcgcaaacc agccgaggga aaaacacttg aaaatctcaa
     7141 tttcaaggat gctttcctca ttggctgcgc acaaacgctc gcccttatcc ccggcaccag
     7201 ccgttccggt atcaccatga ctgccgcacg ctttctgggc tattcacgca ccgaaagcgc
     7261 acatttctca ctgctactag ccattattgc tatcaccggc gccggaacgc tggccagcct
     7321 tgaactttac caatccggcg atataacctt gggccttgac gctttgctcg ccacaatcct
     7381 cgcttttatc gccggatggg cttcaatcgt gctcatgatg aaatggctag agcgcgccag
     7441 cttcacaatc tttgccattt accgcatcat cttgggcgcc gttttactca taatcctcaa
     7501 caccggaatt ttctaaagtt gaccggtata attcaagaat tgaacctgcg tatcaccgta
     7561 ggttttggtt gattgaaccg taaatgaacc actcatatta ggcgcataac ccttctcgct
     7621 ctccagcaca atcacggccg ccgtatcgaa acactcaccc tcctccaagg cctgtaaaac
     7681 aatatcaatt agaccttttc gatacggagg gtctagaaat acaaggctgt atttaggcat
     7741 acttgagggt cttatgaacc gctgtgcatc acgcaagaaa aactcacttt gcagcgccgc
     7801 gtccaaacgt tcggcattac tacgcgtcaa cgccaaagag cgcttgtctt tatccataaa
     7861 gctgcaaaac tctgcccccc gagaaagcgc ctccagcccc agcgcccctg ttccgcaaaa
     7921 acaatcgagc acccgcgcac cgcgcaccgc gcccatagac tccaaagcgt taaaaactgc
     7981 accgcgcacc ttatcgctgg taggacgaat atcccggcct ccgggaacct ccaaccgccg
     8041 tccaccatat tttcccgcca caatccgcat cacacatgac ccttaaaata tcccggaatc
     8101 tgcgccttca tcacgtgcgc ctccacttcc tgcacaccgc cacacggcaa tttattcaat
     8161 tcgaacggac catacccaac gcgaatcaac cgattcacct gcaaccccaa tgcccccata
     8221 acccggcgga tctcgcggtt tttcccctcg cgtaaagtca ccgagatcca acgattagcc
     8281 ccgcttttcc ccgtctgctc aagcttggct tcaatcgatt tatagcgcac cccttcaacc
     8341 gtaatcccgc ctttcaaatc cgccagccgg gcttcatcaa ccgcgcctaa aacccgcaca
     8401 cgataacgcc gggaccaacc cgttgcaggc agctccaagt agcgtgacag gcccccatca
     8461 ttggtcagca gcagcaaccc ttccgtattc agatccagcc gccccacgct cataacccgc
     8521 ggtaattctt taggaagcat atcaaacacc gtcttacgcc cgcgctcatc cttatgcgta
     8581 ctcaccagcc cggatggctt gtgataaaga aacagccgcg cctcctcggc cagacccagc
     8641 agcctgccat ccacctcaat cctatcatcc acgccaacaa cgcacgccgc gctcgcaagc
     8701 cgctcaccat taacctgaac acgcccggcc tcaatccagc actcagcctc acgccgcgaa
     8761 cacaagcctg cccgcgccat aacttttgca atccgctcgc ctttgtggtt ttcttcactc
     8821 atgacatctc atacctcaga tcaggaccat atctatatgc gcgccgcatt ggaagaggca
     8881 aaaacagcgg aggccttgga cgaagtcccg atcggcgccg ttctggtaag ccctgaaacc
     8941 ggtgaaatta tcgccgccca cggcaaccgc acccgcacaa tgtgcgaccc cactgcccac
     9001 gctgaaatcc tgtgtatccg cgagatctgc tctttcgaaa aagcccagcg cacaccgggc
     9061 tatgacctct acataaccct tgagccttgc gccatgtgcg ccgctgcaat ctcatttgca
     9121 cgcctgaacc gggtcatttt tggcgcacat gacccaaaag gcggcggcat cctgcacggc
     9181 ggaaaattct acgaccagcc gacctgccac caccgcccag ccgtaaccca cggcatcctc
     9241 gccgacgaat gcagccagat cttgcgcgat ttttttaaag cgaaacgcta attaggcagc
     9301 ttcttccgcc ttcaaagccc gccaaccgat gtctttacgg caaaacccat ccggccaatt
     9361 aatcttgttc acacccatgt aagcccggcg ctgcgcctcc gcaattgtct cgcccagtgc
     9421 cgtaacccct aaaacgcgcc cacccgtgct aataatctgc ccgttttcat ccttggctgt
     9481 tccggcgtga aacaccgtca catccttcac ttccgccgta tcttccaatc ccttaatcac
     9541 tgtgtttttc ggataagatc ccggataccc ctttgtagcc atcaccacgc acagcgctga
     9601 tttatcgctc catgaaaccc gatcggcaat ttccgccaaa cgcccatgtg ccgccgcatc
     9661 cagaatttca agcaaatccc cttgcaaacg catcatcaac ggctgacact ccggatcacc
     9721 aaaccgcaca ttatattcca gcaatttcgg ctcaccatcg accatcatga ttcccgcata
     9781 taaaacgccc gtaaacggac aaccctctgc agccatcccg tcaatcgtcg gctgaataat
     9841 gcgctcaagt atcttctgct ccagcgcatc gtccattaaa tgcgccgggg aatatgcgcc
     9901 cattccaccg gtattcggcc cttcatcgcc atcataggca cgcttatggt cctgcgcgga
     9961 acttagcggc aatacgctct tgccatcggc cagcgcaaaa taactcagct cctcaccatc
    10021 cataaactct tcaatcacaa tttctgtacc agcctcacca aagcttccgc cactcagcat
    10081 ctcgcgcgcc gcttcaacag cctcagcctt gttatcacac ataatcacgc ccttaccggc
    10141 agcgagccca tcggctttca ccacaacatt gccgggttga tcttcaataa attgctgcgc
    10201 cgcttcaata tccgtaaatc ggccatagct ggccgtcgga atatcgtatt ttgcgcacaa
    10261 atctttcata aaacctttgg aaccctcaag ctgcgccgcc tttttatccg gcccgaatac
    10321 tgcaatattc actgcagcaa gcccgtcagc caaccccgcc accagcggcg cttcaggccc
    10381 aatcaccacc agatcaatcg ctttatcttt ggcaaaaccc accaatcctt gtacatcttc
    10441 cgcaccaata cccacgcact ccgcgcaagc ctcaataccc gcatttcccg gcgcacaata
    10501 tagcgcatta catttttccg acgctgccaa agcccacgcc aacgcgtgct cccgtccacc
    10561 tgaaccaacc aataaaacct tcattttcca atccttctta taaaagaaaa cgctttggat
    10621 aataaactat tcgcttaagg atacaagaga cgcattcccc ccggctgcag ttgtatcaac
    10681 gctgatcacc cgctcgctcg cgaagcgcgc cagataatgc ggcccgccgg ctttcggccc
    10741 cgtacccgac aacccgcttc cgccaaaggg ctgcgagcca accaccgcgc caatcatcga
    10801 acgattcaca taaacattcc cggcacgtac acccgccgcc acatactgat gaaaactctc
    10861 gagccggcta tgaattccca gcgtcaaacc atacccgctc gcatttatat ccctcagcaa
    10921 atcatccaaa ccatctttag aatagcgaat cacatgcaaa atcggtccaa aaacttcttt
    10981 ggtgagcgcc cccatgccgg gaatttcata tgcgcacggc ccaaaataat gccctttacg
    11041 tgcaagcgca tttgagaccg taaccttacc aatacactga ccaaaccctt tcaaagcttc
    11101 atgatgatgc acaagcatag ccatagcctc ctcatcgatc actggtccga catccgtaga
    11161 aagccgagca ggatcaccaa tacacagctc atccatagcc ccggccagca tccgcaccac
    11221 cttatcggca atatcctcct gtacacataa aacccgtaac gccgagcaac gctgacccgc
    11281 gctgccaaac gcgctcaaaa tcacatcatc cacaacctgc tcaggcaatg ccgaactatc
    11341 aacaatcatc gcattctgcc cacccgtctc ggcaatcagc ggtacaatcg gaccattctt
    11401 ctcagccaaa gcccggttta taatccgcgc cacctcggtc gaaccggtaa aagcaacccc
    11461 ggcacacagc ccgtgctcca ccgcgcacgc acctacgcgc ccgtcaccag gcagcaaatg
    11521 taatgctgca ggaatcacgc cagcctcatg cagcaattcc accattttat aagcaatcag
    11581 cggcgtctgc tccgctggtt ttgcaatcac cgcattcccc gtcatcaacg ccgcagccac
    11641 ctgcccggta aaaatcgcca aaggaaaatt ccatggggat atacacacaa acaccccgcg
    11701 cccttgctga taaagccgat tccgctcccc ggtcggcccc ggcaaagctt cgccctcagc
    11761 cgcaaaaagc ttgcgccctc gacttgcgta ataacgcagg aaatcgaccg cctcacgaat
    11821 ttcggatacc gcgtccacca acgttttccc ggcttcgcgc accagcaaag ccataaattc
    11881 accgcgccgc tcttccaaca tgtccgcagc gcactccagc gcccgcgccc gcacatccga
    11941 atcgcacgca ttccactcca caaaaccagc ttgcgcagcc tcaaacgcac gctccaccgt
    12001 tttgaaatcc gcatcataaa cctgccccac cacatcggcc cgatccgccg ggttcaaagc
    12061 cgtccgcgca gccccgcttt tcaatgtcct tccatcaatc aatgctgccg cactgacaga
    12121 actgttatca tacgcaccaa tttcctgact caattgtgca agcgtctcgg gcgcatcaag
    12181 gtccacacca gaagagttgg ctcgaccttg cggcgtttca tccataaata aattttttgg
    12241 caacacaatt ttagggtgct taaactcttt gcggttttta gacttatcca caggatcaac
    12301 cacaagactt tccaccgatg catccgggtc cagcccccgg ttcacgaagg agctattggc
    12361 cccattttca agcaaccgcc gcaccagata aggtaacaga tccttatgcg gcccaaccgg
    12421 cgcataaata ctcgccaccg ccccgcgatc taaaagttga tcaaacagcg cgccccccat
    12481 accaaataaa cgctgaaatt caaatgcgcc cgctccaaca ttatgtgcca tatccataat
    12541 cgccgccacg ctgtacgcat tatgcgttcc aaacatcgga taaagcgtat ccacgtaatc
    12601 aaacatttta tgcgcacaca ccaaatacga cgtatcgcta tggctcttgc gcgtataaac
    12661 cggaaaatcg caaagacccg ccacttgtgc ctttttaatc tcgctgtccc aatatgcgcc
    12721 cttaaccagc cgcatctgaa tgcgccgagt acgcgccttg gccatttcag ccacatgatc
    12781 aatcaccgcc ggcgcagcct tgtgataggc ctgcaccgca agcccaaacc catcccaatc
    12841 tcccaaggat gcatcaccca aaacccgctc aataatctca atcgacaaat tcagccgtgc
    12901 gcattcctcc gcatcgaccg tcaacgccag atcgtacgaa gctgcctttt gcaacaaagc
    12961 caataaccgc gccgccattt cagggacgca gatctcgctc tgcgcaaatt cataacgcgg
    13021 atgcaacgca gaaagcttaa ccgaaacccc gggacgccga accagctttt tatcggcccg
    13081 ctggccgata tactcaatcg cactttcgta cgcctcataa taatgctgcg catcagccgc
    13141 actgcgcgcc ccctcgccaa gcatgtcata cgacattcga tagccgcgat tcctgagcgg
    13201 caaagccttt tgcatcgcct cttcaatatc ttggccaagc acaaactgcc cgcctaacat
    13261 ccgcatggct ttgagcatcg cctcgcgcac aatcggctca ccgattttac tcaaagcgcc
    13321 attcaaagtc ctggaagtca taaacaaccc gaccccggca gctttaacca cccaatcctt
    13381 cgatccgccc acaccctcaa gccaattcgc cgccgcaacc ttgtcccgaa tcaactcccg
    13441 gcgcgttgcc ttatcaggaa tacggagcaa agcctcagcc aacatcatca gagccagccc
    13501 ctcctccgta tccaacgcat aatcacgcat aaaggattcc agctgaccaa cctcctgttt
    13561 ctggccccgc atggcctcaa ttagcgccgc cgcctcttta ccaatccgcg ctgccctacc
    13621 ttcatcccaa tcaaaatctt gaagcaagcc cttaacggca gcttcctcgc tacgctggaa
    13681 ataaggagaa aaggaaggag acgtcattga aaaaaaacct ttcaagcttc aaaggctaat
    13741 cctatcataa aaccatgtcc gaatccgaag cccaaaaaac aaaaatacaa acagaaaccc
    13801 acagatctaa cataccggaa ttttccgtca gcgatctggc gctttcactc aaacgtacac
    13861 tcgaagaaaa ttatggccgc attcgcgtcc gcggagagct ttcccgtatt tcaatcgcgg
    13921 gctccgggca cctttattca tccctcaaag acgatcaggc cgtcatcgat gccgtatgct
    13981 ggaaaggcgt actctccaac ctctccgtta aacctgaaga aggccttgaa gtcatctgca
    14041 ccgggcgtat atccagctac cccaaaagct caaaatacca actgatcatc gaatcgatgg
    14101 aactggccgg agaaggcgca ctcctcaaaa tgcttgaaga acgtaaaaag aaactgggcg
    14161 aggaaggtct cttcgccccg gaaagaaaaa aaccgctgcc cttcctgccc cagaccatcg
    14221 gcgtcgtcac ctcacccacc ggcgcagtca ttcgcgatat tctccaccgc ctggccgatc
    14281 gcttcccgcg ccatgtgctg gtctggcccg tcctcgtgca gggcgacggc gcggctaatc
    14341 aagtcgccag cgccatccgc agcttccaaa ccattcataa acacggccag ccccgccccg
    14401 acttgctcat cgtcgcgcgc ggcggcggct ctctcgaaga tttaatgccc ttcaatgaag
    14461 aagacgttgt ccgcgcgatt gccgaaagcg aaatcccgat catctccgca atcgggcatg
    14521 aaaccgacac cacacttgcc gacttcgcag ctgatgtccg cgcaccaacc cccaccggcg
    14581 ctgcagaaat ggccgtgcct gtacgtgcca acctcatcgc gcaaattctg gaagacgaac
    14641 agcgcctgat ctccggcatg acccgcctac tctccgaaca tcgccacaaa atagaatcac
    14701 tcagcgcccg ccttggtgat cccgcgcgcc tcctcgaaac gcagacccag cggatcgacc
    14761 acctttccta taaactctcc ggacttttta gccgtttcct cgatacaaaa aaacatgcgc
    14821 tatccgagca aaccgccagg cttcgccacc cacgcgaacg cctcgcagaa gcatcacaaa
    14881 atctatcgcg ttggaacgaa catctggaaa cgctcagcct caaactcacc cgggaaccaa
    14941 cacaaaaact ggaccatctt ggtaaaatgc tcgaagcata cagctttgaa aatattctcc
    15001 agcgaggttt tagcgtcgtg cgcgatgaaa aaggaactct tatccccgat gccagtaaca
    15061 tccacccgaa ccaggtactg gacatacagt ttcgtaatca gggccacatc caggcaatca
    15121 caacatcaaa aaaagcttca aagcccaaaa cgaaaaaaag aaaagcgcca ccacctgcgc
    15181 aaaacgaccc tcagacaaac ctgtttgact aacccaaaac cagatttcaa acaaattatt
    15241 cgccgtcttc ttctatggca gaggtggtct cggttcttcg gccagctttg cggcgaagtt
    15301 aagctacgat cgg
//
LOCUS       DAOSWR010000039        15275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2432_length_15275_cov_6.209001, whole genome shotgun sequence.
ACCESSION   DAOSWR010000039 DAOSWR010000000
VERSION     DAOSWR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_15275_cov_6.209001"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..2420)
                     /locus_tag="PK513_04445"
     CDS             complement(390..2420)
                     /locus_tag="PK513_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO81731.1"
                     /translation="MFVFKNSPLKNAKISLQINFIILAVALGFTLTGGIYFYSYTTSM
                     KKGAESNVLVSAKEMNDEIGYDFLNARRNEKDFLLRLDKKYVEKHAATSKKIHNKIKE
                     LGDLLARNGHSSEAVEKVDVGYIKYEAQFETVVKYWLEIGLNPNTGLQGDFYEAADDL
                     EELVNGIKDDKLTALYLKMRGDEKDFMTYLEYEYIEAVENDVAVFSKRLSDLASSQSN
                     QQEGPYLDAQRNISPAIRKEAAKNLDIYLEKFHEMADLRDNIVAQTKKLSSTFAEVQP
                     VLDSFSEEMAAQSKQATEEAKASAKASFDLMVGVMVSVLLFTSLLCVMIGRGISRPIL
                     ALSGVMNGLAEGNLETEVPFAEQTNEIGGMAKALQVFKDNAIETEQMRKEQKIREQRA
                     EEDKKRAMNELADSFEKEIGGIVDTVSSASTEMEATAKAMSESADQTSTKAGIVSSSA
                     EEASANVNSVAGAAEELSASISEISSQVASSAKVAAEAKQKADQTAERVQSLVSAAER
                     IGEVVVLISDIAEQTNLLALNATIEAARAGDAGKGFAVVASEVKSLASETSKATEEIS
                     NQISGIQSATRESDGAIKEILEVIKRIDEISGAVASAVEEQGAATSEITRNVQQASQG
                     TSKVTENISEVREAANDTGQSASMVLDSAKELAKQASSLGNTVSNFLNRVRT"
     gene            complement(2538..2912)
                     /locus_tag="PK513_04450"
     CDS             complement(2538..2912)
                     /locus_tag="PK513_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81732.1"
                     /translation="MKKTLGLFVATIVLAGFSTANALAADCVVHYKRTACAGQEEISY
                     KKCGGEQECEKTKPAESKEECMEAALKSCDNKRFDITKWKVITATYGDEQLIGGHDED
                     GAPNPEGTNFCAGDRPDLNQCE"
     gene            3281..4687
                     /locus_tag="PK513_04455"
     CDS             3281..4687
                     /locus_tag="PK513_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOO81733.1"
                     /translation="MFRNALLLTSASGLIFAASPALAEQMSMEEIQAQLQQLSAQVQN
                     LSKVVEQQNEVIKNQEAALQAQKQASAEAISEAVANIQPAAGGVVGDVKISMKPSPKI
                     ESADGKYSFQPFGRVHLDTTQFDDDAFDYANNSNFRRARLGFKGNLGEDFNYKSEIDF
                     AGEAVAFKEVTLTYTGLDAADIKVGHQKPSVGMEQNTSSNYLMFIERSAPTNAFTRDE
                     EIGVNVLAGGDNWSLGAGVFNEDGGNSGTGEDEDVTFDVRGSVNVLGLGSNASDSVLH
                     LGAGYSHREPTGNVRFRARPGTGDGARTIDTGNFGSVDDVGVYNAELAAVFGPVSFQG
                     EYLKADVSRSGGNQDADFDGYYAQAGWFLTGESRPYNGKIGNFKRVKPKNPFSLKNGG
                     WGAWEVLARYDNTDLNDASAGITGGEMDTVSVGVNWHLTDHIRLMANVIDVDSDANAA
                     TAANDDPTVYNFRAQWDF"
     gene            complement(5596..5847)
                     /locus_tag="PK513_04460"
     CDS             complement(5596..5847)
                     /locus_tag="PK513_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81734.1"
                     /translation="MPSQRTGHRHSVRDPRQAENPVRSAVDPVSGNTVNKAEAVIGAG
                     PDKTVYYFENEENFQKYASGPMPEMSHDHGAMDETMHHH"
     gene            complement(5901..6695)
                     /locus_tag="PK513_04465"
     CDS             complement(5901..6695)
                     /locus_tag="PK513_04465"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOO81735.1"
                     /translation="MYDFLTAIFVGIVEGLTEFIPVSSTAHLIVLVDGLNFPSPPGHV
                     FEVFIQIGAIFAVMWLYRVKLFHTAMCILREKQSQIFALNLILGSMPALLIGALFHDA
                     IKTMLYNPHVIAFTLIIGAIIMLIFEKRFTNTTVENVDAITPRKALLIGCCQMLALIP
                     GVSRSGATIMGGLTFGLSKPAATEFSFFLSIPVMFAAVLYDTYKNREAIMAFDQTGLL
                     AAGFIAAFLSALLVIKFVVNFISRHGFVPFAWYRIAAGIVIAVVFF"
     gene            complement(7126..7581)
                     /locus_tag="PK513_04470"
     CDS             complement(7126..7581)
                     /locus_tag="PK513_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRASH domain-containing protein"
                     /protein_id="HOO81736.1"
                     /translation="MKKLLMTLILAIMTVPAMAQEHSHDHAADWLKPVEAQFVCMVNN
                     QSYDTPQIAVEVEGKTYYGCCPMCKDRLAKDPAVRSAVDPVSGNPVNKAEAVIGAGPD
                     KTVYYFENEENFQKFASGPMPEMNHDHMEGMDHSMMNGDNTPQKHEDHH"
     gene            complement(7608..8228)
                     /locus_tag="PK513_04475"
     CDS             complement(7608..8228)
                     /locus_tag="PK513_04475"
                     /inference="COORDINATES: protein motif:HMM:NF021505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HOO81737.1"
                     /translation="MMMNMEIIPNWHPILVHFTIALLSVSAVLFIAEIFVRKQSWHIQ
                     LVTVARWNLWLGALATIATVIAGFYAFNSVPHSSEHQHLAMLDHRKWALSTAALFVLL
                     ALWSFSVTLRVKADFGKGTNLVFVGLITIAGLMLATTGYKGAELVYRHGLGVIPMQMS
                     MAGGHHHSHGGHEHTEKSPEEHHDTMPSTEMPDTDESHHDHNAHQH"
     gene            complement(8245..8976)
                     /locus_tag="PK513_04480"
     CDS             complement(8245..8976)
                     /locus_tag="PK513_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HOO81738.1"
                     /translation="MRTKLFFTTSLFLLAMAMPAFAEEAHNHNPADHQHGSEAYHMFR
                     VETDYGAGEHGPVARFDIDGWYGTDENKLWLRSEVENSDGVTEKAEFQALYSRNVATF
                     WDLQAGLRHDTQPTSTTYAVFGVEGLAPYFFETEAHLFVSDEGDVTARLREENDFLVT
                     QRLILQPYAEVNLSAQDVEDQEIGAGLTDGEIGLQTRYEFTRKFAPYIDVRYERKFGE
                     TSSIAKSNGEDNDNFIAGVGLRLMF"
     gene            complement(8966..10006)
                     /locus_tag="PK513_04485"
                     /pseudo
     CDS             complement(8966..10006)
                     /locus_tag="PK513_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
     gene            9897..10937
                     /locus_tag="PK513_04490"
     CDS             9897..10937
                     /locus_tag="PK513_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOO81739.1"
                     /translation="MTSYVSATPKRNSSTETGSTFCPSDDTTCIFRPGILTAGYPCQP
                     FSIAGSKRAEKDPRHLWPHIKDIVGVIEPEICFFENVSHHLRLGFQQVHDDLRSMGYS
                     VAAGLFTAEEVGAPHKRERLFILAYRDRDRCLGEHGTACGENDPQGRSQCAADTIAGG
                     DSHATGRLPVHETGSGKTEIRSGLQDGESPVADTSGARARVEQAAVQQGAVTDSTAMA
                     DPAGEREWREAGNLPEADERPDGRSPQFSDGTSQSMANAMRSGLEGFPRHERPEDAAD
                     FVTVSGFPVWPPSPGDREQWGYVADAFKPAVHRMADGMAFRVDRLRLCGNGVVPLVAA
                     YAFRILLAALRA"
     gene            11232..12488
                     /locus_tag="PK513_04495"
     CDS             11232..12488
                     /locus_tag="PK513_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOO81740.1"
                     /translation="MHELKVEYRSINDVTPYEGNARAHSEQQIKQIERSIMRWGFYNP
                     IKVDQNGIIIAGHGRLKAAQNLGLKEVPVIAVHCETEADRRALTIVDNSIAENATWNE
                     QLLKIEAEFLREVEDFDLEDLALDPVFLDELLLSDGSEDDQGEGEEENVPEPPDDPVS
                     RVGDLWLCGRHKVLCGDSTKKEDYEKLFGAEELADMVFTDPPYNVNYGRTEETRARGK
                     GKKIKGREIKNDNLGAAFGKFLSDLCALALEYTKGAIYICMSGSEMANLEKAFTENGG
                     HVSTQIIWAKNHYTLGWSDYHKQYEPIMYGWRKGNDHYWCGARDQSDLWHVNRPQVNT
                     VHPTMKPVALVEKAILNSSKTQDIILDPFGGSGTTMIAAENTGRYARLIELDPKYVDV
                     IVERWQEATGKTAIHADTGKAFKMND"
     gene            13035..13190
                     /locus_tag="PK513_04500"
     CDS             13035..13190
                     /locus_tag="PK513_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81741.1"
                     /translation="MKSNTQPKIIKDDLPIKKPPQKQWIDKRAANQKEPKYLLPKEED
                     CSNRATD"
     gene            13194..13454
                     /locus_tag="PK513_04505"
     CDS             13194..13454
                     /locus_tag="PK513_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81742.1"
                     /translation="MITSPQLRAARALLGWDQKQLAELSGLSLPTIQRMESSDDMVRG
                     NVDSLTKIISALNEAGVELIGESVISTGQGRGVRLINKSEAS"
     gene            13457..15139
                     /gene="sulP"
                     /locus_tag="PK513_04510"
     CDS             13457..15139
                     /gene="sulP"
                     /locus_tag="PK513_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOO81743.1"
                     /translation="MIPAHFTPKLFSLLRQGYNFDTFKSDVMAGLTVAIVALPLSMAL
                     GIASGASPDKGLITAVIAGFLISFLGGSRVQIGGPTGAFIVVIFNVIAQYGYDGLILA
                     TLLAGIILVIAGYAKFGRVVKYIPYPVVTGFTAGIAVIIASSQIKDFLGLHVPEVPAE
                     FIAKWQSYFLHIDTFSLITLIVGLAALLTIILLKRFAPKLPGYLIAVAITALTVFVFH
                     LPVETIGSRFPDMPSGLPMPHIPAWDFEKFISIVPSAFTIAFLAGVESLLSAVVADGM
                     TGYKHRSNQELVGQGVANIASAFFGGMPATGAIARTATNIKAGGKTPMAGMFHALFIL
                     LFILFATDIMAFVPLAALSAILFIVAWNMSEFQHFIHIFRLSKSDRTVMLLTFALTVL
                     VDLTVAIGVGVTLASLLFMREMSKSVVLRSQGRHIRDIFNDNEEDVEQRDDLPVGVEV
                     FRITGPLFFGVAGTMLETLQSMGEVPKILIIRMKLVPYMDVAGLNAMRNLMKGCHTKH
                     TKVILSGLQPQPGDMLDKADIKDNHTNLWISASYDDAIALAHQLVAEPTVSM"
BASE COUNT         3946 a         3728 c         3575 g         4026 t
ORIGIN      
        1 gaacctccgc atggcgatac caaaaaccgc tgccttaccg cttggctaca ccccagcaag
       61 aaatgtacac atatggcaga cggtgccgca acacggactc aaaccgcaga cctgacgatt
      121 acaaatttta tttttgcaag cctcaggaca gcacacaatc tctcgtaaca tcactgctga
      181 ctgttattta atgaaaagct acattttcgt caagctttat aaaaactcat aatgtttcac
      241 tgataattgc ctcattcgtt acccaaatgt tacccaaaat ttcatgggct tatttataag
      301 ggacaggtca gtttacgttg gctcggtctt ttcatttaag aactttattc aaatatcaaa
      361 gaacacaggg accgatgaac aaaaggaaat cacgtcctga cacgattcag aaagttactc
      421 accgtgttgc caagagaact cgcttgcttt gctaactctt ttgccgaatc aagaaccatg
      481 ctcgcggatt gccctgtatc atttgcggcc tctcttactt cgctaatatt ctcggttact
      541 tttgaggttc cctgcgaagc ctgctggacg ttacgtgtaa tctcgctcgt agcggcgcct
      601 tgctcttcca cagcagaggc caccgctccg gatatttcat ctatacgctt gataacttca
      661 agtatctctt taatagcacc atcagattca cgtgtagcac tttgaattcc agaaatctga
      721 ttagaaatct cttcagttgc tttagacgtc tcactagcaa gggatttaac ttcgctcgcc
      781 acgaccgcaa atcctttacc ggcatctcct gctcttgctg cttcgatagt cgcatttaag
      841 gccaaaagat ttgtttgctc agcaatatca gaaatgagca caacaacctc gccaatgcgc
      901 tctgctgcgc tgacaaggct ttgcacccgt tctgcggttt ggtcagcctt ctgctttgct
      961 tccgcagcaa ctttcgccga gctggcaact tgagaagaaa tttcactaat cgaggctgaa
     1021 agctcttcag ctgcgcccgc aacagaattc acattcgcag acgcttcttc cgcagaggaa
     1081 gaaacaatac cggctttagt gcttgtctga tcggcacttt cagacatggc ttttgccgtc
     1141 gcttccattt ctgttgaagc agaagaaacc gtatcaacga tcccaccaat ctccttttca
     1201 aaggaatcgg ccagttcatt catagctctc ttcttatctt cctctgcgcg ttgttccctt
     1261 attttctgct cttttcgcat ttgctccgtt tcgatagcgt tatctttaaa aacttgcagc
     1321 gctttcgcca tacccccgat ttcgttggtc tgctctgcaa aggggacctc tgtctccaga
     1381 ttgccctctg ccaatccatt cataacacca gataatgcga gaatgggacg agagatgccg
     1441 cgcccaatca tcacgcatag caggcttgta aataacaaca cggaaaccat aacaccgacc
     1501 ataagatcaa aacttgcctt agcgcttgct ttagcttctt ctgtcgcctg tttcgattgt
     1561 gcagccattt cttccgaaaa actgtccaga acaggttgca cttcggcgaa agtcgaagaa
     1621 agtttttttg tttgcgcaac aatattgtca cgcaaatccg ccatctcatg aaatttctca
     1681 aggtatatgt ccaggttttt ggctgcctct tttcggatgg ctggagatat attccgctgt
     1741 gcgtctaaat aggggccttc ttgttggtta gattgggagg aagccagatc cgataatctt
     1801 ttcgaaaaaa ccgccacgtc attttctaca gcctcaatgt attcatattc cagatatgtc
     1861 ataaaatctt tttcatcacc gcgcattttc aggtacaagg cggtgagttt atcatctttt
     1921 atgccgttca ccagctcttc aagatcatca gcagcctcat aaaaatctcc ctgaagaccg
     1981 gtgtttgggt ttagtccgat ttcaagccag tatttcacga cagtctcaaa ctgagcctcg
     2041 tattttatat aaccaacatc aactttttcc acggcttcgg atgagtggcc gtttcttgcc
     2101 aaaagatcgc ccaattcttt gattttatta tgaatttttt tgctggtggc ggcatgtttt
     2161 tcaacatact tcttatccag acgcagtaaa aaatcctttt cgttgcgccg cgcatttagg
     2221 aaatcataac cgatttcatc gttcatctcc ttagcagaga ccagaacatt actttcagcc
     2281 ccctttttca tacttgttgt gtagctatag aagtaaatcc cgccggtgag agtaaaacca
     2341 agcgccaccg ccagaattat aaaattaatc tgcaggctga ttttcgcgtt cttcagggga
     2401 ctatttttaa aaacaaacat ggggggggga gcctctgcat gatatcaata gaagttaatt
     2461 attacgcaga aaactcagca cccttatatg cggggaaaga cgcggcccat attccaaaac
     2521 cgcatcttat ttatttctta ttcgcactga ttcaggtcag ggcgatcacc ggcacaaaaa
     2581 tttgtacctt ctggattggg cgcaccatct tcatcatggc cgcctataag ctgctcatca
     2641 ccataagtcg ccgtaataac cttccatttg gtaatgtcaa agcgcttgtt gtcacagctc
     2701 ttcaaagctg cttccataca ctcttctttt gattcggctg gcttcgtttt ttcacactcc
     2761 tgctcaccgc cgcatttttt gtaggatatc tcttcctgac cggcacaggc ggtacgctta
     2821 tagtgaacaa cacaatcagc agccaaagcg ttcgctgttg aaaagccagc cagaacaatt
     2881 gttgcgacaa ataaaccaag tgttttcttc attatatttc tccttaacgg ttagttatta
     2941 accatatgtt ttacatccat tcatcgtcat gaagaacggt ctctttttta tgacaacgaa
     3001 tccctgaacg caatgagcat gcagcaacat ctcgtcgaat caaggggata aactttagga
     3061 actatcccgc caacaccacc agacttagca acacgccttc ctatgcgaca tcctgcgcag
     3121 aaattcaaaa ataaacacta ttaaaaaata atattatata tgaattaatt tgcagctaac
     3181 acgccaccat cttgcccctt tattcgaaaa atgtaaactc aacattcgag ggggtgattg
     3241 tatccccaac ttcaatctac agaaaataaa ggataccgtc atgtttagga acgccctttt
     3301 acttacgtct gcgtctggtt tgatttttgc ggcaagcccg gcgctagccg agcaaatgtc
     3361 gatggaagaa attcaggcgc agctgcagca attatcggct caggttcaaa acctatccaa
     3421 agttgttgaa caacaaaacg aagtcattaa gaatcaggaa gccgcgttgc aagcgcaaaa
     3481 gcaagcctct gccgaagcaa tatcggaggc ggttgccaac atacaaccgg ctgcaggagg
     3541 cgttgtcggc gacgtcaaaa tttcgatgaa gccatccccg aaaattgaat cggcagacgg
     3601 aaaatactct ttccaaccat tcggtcgtgt acacctcgat acgacacagt tcgacgatga
     3661 cgcttttgac tacgccaaca attcaaattt ccgccgtgcc cgccttgggt ttaaaggcaa
     3721 tctcggcgaa gacttcaatt ataaatctga aatcgatttt gccggcgaag ctgtcgcttt
     3781 taaggaagtg accctgacct ataccggact tgatgctgcg gacattaaag tcggtcacca
     3841 aaaaccatcc gtaggtatgg aacaaaacac aagctccaac tatctgatgt ttattgagcg
     3901 ctccgctccg accaatgctt tcacacgtga tgaggaaatc ggtgtgaatg ttctggccgg
     3961 cggggacaac tggtccctgg gtgcgggcgt cttcaatgaa gatggaggca atagtggaac
     4021 gggtgaggat gaagacgtta ccttcgatgt tcggggctct gtaaatgttc tgggtcttgg
     4081 cagcaatgcc tctgacagtg tattgcatct tggtgccggt tatagccacc gcgaacccac
     4141 aggcaatgtc cgcttcagag cccgtcccgg aacaggcgac ggagcccgca cgatcgacac
     4201 agggaatttc ggttccgttg acgatgtcgg cgtttacaac gccgaactgg ccgccgtgtt
     4261 tggcccggtc agtttccagg gtgagtattt aaaagcggat gtcagccgta gcggtggaaa
     4321 tcaggacgct gattttgatg gctattatgc ccaggcaggc tggtttttaa cgggcgagag
     4381 ccgcccttac aacgggaaaa tagggaattt caaacgcgtg aaacctaaaa atccgttcag
     4441 cctgaaaaat ggcggctggg gcgcgtggga agttttggcc cgctatgata atacggatct
     4501 gaatgatgca agcgcgggta ttaccggcgg agaaatggat actgtgagcg tgggtgtaaa
     4561 ctggcacttg acggaccata tccgcctcat ggctaatgtt attgatgtgg attcggatgc
     4621 taacgctgcc accgcagcaa atgacgatcc aacagtttat aacttccgcg cacagtggga
     4681 tttttaaatc aaaagcgacc ccttttaatt acaaaaagct ccgcgaacat gcggagcttt
     4741 tttataaaga cactttaagc caaaaactca cgcaggaaca actggccgtt ctggcgggtg
     4801 tgagcaagcc gacgctcaac agtttcgaac agggcaagac aacgataaag ctggagagcg
     4861 cgctcaagat tttaaaggtg ctgggtctgg cataaggaaa attgcaaaca tactctcatg
     4921 attttttgga cgaatcttcg aaaatcaccc gtgacatttc cggaaaagta ttgtagtttc
     4981 cagcccatag caaaaggccg cggtagctca gtggtagagc gcacccttgg taattaagaa
     5041 aaattgcctc ctcgcttgga aactggcgag agaacactgc tcaaattcgg ggaaacctcc
     5101 cggacttaaa caaagtccat ggcaatcccg agccaagcct cttagaatgg gggaaggtgt
     5161 agagactaaa cgggcagcac ctaaagcccc ggacttgatc cgggggtatg gtgaagagat
     5221 agtccaggct gcaaacgccc caaatttcat atgatttgag gggcggcggt gaaagccgtg
     5281 gcggtatgaa gggtgaggtc gagagttcaa ttctctctcg tggcaccatt ttaacaatta
     5341 cgaactttgt ctacgctttt tcacctcccc tgtgaagcct agtatatgta aggttgccct
     5401 gtgaatttac cgcccaaaag accacaccct tcggggagag attctctctc tggggccgtt
     5461 tttctctcaa agggtgtgac ctcgcgtaat tgagtcacac ccctaagaat tgttattttt
     5521 aagtatgttg caatgtatta aaaaattaac ggtttaagga tgctctgcat accaatagat
     5581 aggagaggca aacttttaat gatggtgcat agtttcatcc atagcaccgt gatcatggct
     5641 catttccggc atcgggccgc tggcatattt ctggaagttt tcttcattct cgaaataata
     5701 gaccgttttg tccggtccgg caccaataac agcttcggcc ttatttacgg tattgccgct
     5761 aaccggatcg acagcagaac gaacagggtt ttccgcctga cgcggatcgc ggactgaatg
     5821 acgatggcca gtacgttgac tcggcatacg cgagagctgg atagcaaggt catcaatttc
     5881 atgaagaacg tgaaagcagc ctaaaagaaa acaaccgcaa tcacgatgcc tgctgcgatg
     5941 cggtaccaag cgaacggcac aaagccgtgg cggctgataa aattcacgac aaactttata
     6001 accagcaggg cgcttaggaa ggcagcaata aaacctgcgg ccaaaagccc agtttggtca
     6061 aacgccatga ttgcttcgcg gtttttataa gtgtcgtaga gaacagctgc aaacatcacg
     6121 gggattgaaa gaaagaatga gaactctgtt gccgctggtt ttgaaaggcc aaacgttagc
     6181 ccgcccatga tggttgcgcc cgagcgcgaa acaccgggaa tgagcgccaa catctggcag
     6241 cagccaatca gcagcgcctt gcgcggggtg attgcatcga cattctcgac cgtagtgttt
     6301 gtaaagcgtt tttcaaaaat caacatgatg attgcgccga taatcagcgt gaaggcaata
     6361 acatgtggat tatagagcat ggttttgatg gcgtcatgaa acaaagcgcc aataagcagg
     6421 gctggcatgc ttccgagaat aaggttgagt gcaaatatct gtgattgctt ttcacggagg
     6481 atgcacatgg ctgtgtgaaa aagtttaacc cgatagagcc acatcactgc aaaaatcgct
     6541 ccgatctgga tgaaaacctc gaacacatgt cccggcggcg aggggaagtt caggccatcg
     6601 acaagcacga tcaaatgcgc agtcgaggat acggggataa attccgtcag gccttcgacg
     6661 atgccgacaa aaatggcggt aagaaaatca tacatcggtg ggagtattcc gtttatttga
     6721 gttatggtgg ttccgactgg acttgaatcc gcatggcctt acggccaacg gattttaagt
     6781 ccgttgttat aatattgcac ccttgattta atttaacaat ttcatttttt ataaaaaagc
     6841 gtgtaagttt ttgtgcgctt gtgtaagcca aaatctgctg cgcctaagtc agccgtagac
     6901 gcttacacag cgtttttcag actgataagt atagagatgt taccaccttt caaaagaccc
     6961 aaaattccga tttatagact taggcagaag ttagtcacac taaacgcagc cgcaaggtat
     7021 ttgctatcac gcttatgatc ccagcatggg tgcgcattat aatcatactc atgatgagag
     7081 tgatgcgatt tatcctgatt cattttttct ctcttaaata tagagttaat gatggtcttc
     7141 gtgtttttga ggtgtattat ctccattcat catagagtga tccatgcctt ccatatgatc
     7201 atggttcatt tccggcatcg ggccgctggc gaatttctgg aaattttctt cgttctcgaa
     7261 ataataaacc gttttgtccg gtccggcacc aataacggcc tcggctttgt tcacgggatt
     7321 gccgctaact ggatcgacag cagaacgaac agcggggtct ttagcaagcc tgtctttgca
     7381 catcgggcag cagccgtaat aggttttacc ttcgacctct acagcgattt gcggcgtgtc
     7441 gtagctttga ttattcacca tacatacaaa ttgtgcctcg acaggcttca gccaatctgc
     7501 ggcatgatcg tgggaatgtt cctgcgccat ggccggaaca gtcataatcg cgagtattag
     7561 tgtcataagt agttttttca ttttctttct cctttttaga gttggggtta atgttgatgt
     7621 gcgttgtgat cgtgatgact ttcatccgta tcaggcattt ccgtgctcgg catggtgtcg
     7681 tgatgttctt ctgggctttt ttcagtatgc tcgtgtccgc cgtggctatg gtgatgacct
     7741 ccggccatac tcatctgcat gggaataacg ccaaggccgt ggcggtaaac aagctccgct
     7801 cctttatatc cggttgtcgc cagcataagt ccagctattg taataagccc tacaaagacc
     7861 aaatttgttc cttttccgaa gtcagctttg acgcgcaaag tcacagaaaa agaccatagt
     7921 gccagaagca caaacaaggc agcggtgctt aaagcccatt tacggtgatc gagcatggca
     7981 agatgctgat gttcactgct atggggaaca gaattaaagg cataaaatcc ggctatcacc
     8041 gtagcaatag tggccaaagc cccaagccat aaattccacc ttgcaaccgt tacaagctgg
     8101 atatgccagg actgtttcct gacaaatatc tctgcgataa acaacacggc agagacgctt
     8161 aaaagcgcga ttgtaaaatg caccaatatg gggtgccagt ttggtataat ttccatgttc
     8221 atcatcatgt ccatgatctt cctcctaaaa cattaagcgc aggccgaccc ctgcaataaa
     8281 gttgtcattg tcctcaccgt tacttttggc aatcgaagag gtttcaccga atttgcgctc
     8341 ataacggaca tcgatataag gcgcgaattt acgggtaaac tcgtagcggg tttgaaggcc
     8401 gatttcgcca tcagtcaaac ctgcaccgat ttcctgatcc tcaacatcct gcgccgataa
     8461 attgacttct gcgtatggct gtaagattag gcgctgtgtt acaagaaagt cgttttcctc
     8521 tcgcaatcgc gccgtaacat cgccctcgtc actaacgaat agatgcgcct ccgtttcaaa
     8581 gaaatacggc gcaagccctt caaccccaaa gactgcatag gtagtagaag tcggctgcgt
     8641 atcgtggcga agccctgcct gtaagtccca gaaggttgca acattacggc tgtagagcgc
     8701 ttgaaattcg gctttttctg ttacgccatc ggagttttca acttcgcttc tgagccagag
     8761 tttattttca tccgtcccat accagccatc aatatcgaaa cgggcaactg ggccgtgttc
     8821 tccggctccg taatccgttt ctaccctgaa catgtgatag gcttccgatc catgctgatg
     8881 atcggcagga ttgtggttat gagcctcttc agcaaaggca ggcatagcca tggccaataa
     8941 gaacaatgat gttgtgaaaa aaagtttagt gcgcatggtt gtgccctcct tgcgtttcag
     9001 tttttgtgtc gtggttcatt tgggagtgat ccattttact atgatccata ccttccatgg
     9061 cgggcatatc cattttcatt tgcccgtgat ccatttgaga atggtccatc ttggaatgat
     9121 ccatgccgcc catatcatga tgcattggct ttgccgctgc attcggccta taaggcgcgg
     9181 cctttgcgta atcagggatg tccgatggat caagtttggc gacaacagct ttcgtcatca
     9241 tgccagcggc catgtggtag agaagatggc agtggaatgc ccattctcct gcctcatctg
     9301 ccgtcagcaa tacagaatag ctcccgccag gcggtacaat cactgtatgt ttattgggca
     9361 gcttttccat cggctggccg ttttcaagct gcacaaacat tccgtgtaag tgcatcgggt
     9421 gcgccatcat ggtgtcattg acaaatttca gtctgacacg ctcaccatat cttaacttca
     9481 tcggcccaga gtcggagtat ttcttgccat tcatcgtcca gatataacgc tccatattgc
     9541 cgccgagacg caccagaatt tcacgctcag gctggcgcac atccttttgt gtgccaagat
     9601 agcgcaggtc ggaatagcta agctgcttat cacccttggg ggtgcccgct ttcgcccagc
     9661 cgctttccat ctcagccatc atatcctcca tttcttctgg cgtcatttta ctatgatcca
     9721 tgccgctcat atcatgtccc atacccatgt cggccatggt taaaagagtg cggaaacgct
     9781 gctcaggaat ttcacccttc atgccgtcac gcggagccag tgtgccaagt gcaaagccac
     9841 tgcgatcaat cggttctgct gtaatcgtat aggctttatc ttccttgggc gttacgatga
     9901 catcgtatgt ttcagccaca ccaaaacgaa actcatcaac agaaacaggc tcaacattct
     9961 gcccgtccga tgacacaacc tgcattttca ggccaggtat cctcactgca ggatatccgt
    10021 gccagccgtt ctcaatcgcc ggatccaaac gcgccgaaaa agaccctcgg cacctctggc
    10081 cgcacatcaa ggatattgtc ggcgttatcg aaccagaaat ttgtttcttt gaaaacgtct
    10141 ctcaccacct acgtctgggc ttccagcaag tccatgacga cttacgatca atgggttaca
    10201 gcgttgcggc gggcttgttt actgcggaag aagtcggtgc gccgcataag cgagaacgct
    10261 tattcatcct ggcctaccgt gaccgcgacc gatgcctcgg tgaacacggc acggcctgcg
    10321 gagaaaatga tccgcaagga cggtcgcaat gtgctgcgga caccatcgct ggcggagaca
    10381 gtcatgcaac cggccgactt cccgtacacg aaacaggatc tggaaaaaca gaaatccggt
    10441 caggattaca agacggcgaa agtccagtgg ccgacacctc gggcgcacga gccagggtcg
    10501 aacaagccgc cgttcagcaa ggggctgtca cagacagcac ggcaatggct gaccccgcgg
    10561 gtgagcgaga gtggcgagaa gcaggaaacc ttcctgaagc ggatgaacga ccggacggac
    10621 gaagtccaca gttctctgac ggcacaagcc aaagcatggc caacgccatg cgctcgggac
    10681 tggaaggatt ccccaggcat gaacgaccgg aagacgcagc agatttcgtt accgtttctg
    10741 gctttccggt ctggccacca tccccaggcg accgagagca atggggatat gtcgcagatg
    10801 cgtttaaacc cgctgttcac cgaatggctg atgggatggc ctttcgggtg gacagacttc
    10861 ggctctgtgg aaacggagtg gtgccactgg tggcagctta tgcgttccga attttgcttg
    10921 ctgctctccg tgcatgaacg gcacatcgca tcgtaatcct gtgttgcggg attgttgcgg
    10981 gctttgtcaa ccaagtgccg aattgtcaac cagagcgatc ctgtcaacca agcggaaatc
    11041 aagtcgaaag attatctaaa gaaatcaaaa ggttgcaatc cctgttctgt caacctgtca
    11101 actaagttgc gaggtctgac gctaaaaaac aaatgcgcgt tcgccgcccg tatccgttcg
    11161 ccgcccacag tacctttttt atttttgcgc tctcgcgcaa caaccccagc aacatgaaag
    11221 cagagtgaaa aatgcacgag ctcaaggtcg agtatcgatc aataaatgat gtcaccccct
    11281 acgaggggaa cgcccgcgca cacagcgagc agcagatcaa acaaatcgaa cgctccatca
    11341 tgcggtgggg cttctataac ccaatcaagg tagaccagaa cggtattatt atcgccggac
    11401 acggtcgcct caaagctgca caaaacctcg ggcttaaaga ggttccggtc atcgctgttc
    11461 actgtgagac cgaggccgac cgtcgtgcgc tcaccatcgt tgacaacagc attgctgaga
    11521 acgctacgtg gaatgaacag cttttaaaga tcgaggctga gtttttacgt gaggtcgagg
    11581 attttgatct ggaagatttg gcactcgatc cagtgtttct tgatgagttg ttactgtcag
    11641 acggcagtga ggacgaccag ggcgaaggcg aagaagaaaa cgtgccagag ccaccggacg
    11701 atccggtgtc gcgtgtgggg gatctgtggt tatgtggcag gcataaggtg ctgtgcggtg
    11761 actccacaaa gaaagaggat tacgagaagc tctttggggc ggaagagttg gccgacatgg
    11821 tgtttaccga ccccccgtat aacgtgaatt atggccggac ggaggagaca cgcgcccgtg
    11881 gtaagggcaa gaaaataaaa ggccgtgaaa ttaaaaacga taatctgggc gcggcattcg
    11941 gcaaattcct ctccgatttg tgcgccctgg cactcgaata taccaaaggc gccatttaca
    12001 tctgcatgtc cggcagcgaa atggccaatc tggaaaaggc atttactgag aatggcggtc
    12061 atgtttccac gcagattata tgggcgaaaa accattacac gctcggctgg tctgattatc
    12121 acaagcaata tgagccgatt atgtacggct ggcgcaaagg taacgaccat tactggtgcg
    12181 gtgcgcgaga tcaaagcgac ttgtggcacg taaaccgccc acaggttaac accgtccatc
    12241 cgacaatgaa gccggtcgcg ttggtcgaaa aggcgatcct caattccagc aaaacgcagg
    12301 atattatcct cgatccgttt ggcggatccg gcacgactat gattgctgcg gaaaatacag
    12361 ggcgttatgc gcgtctgatc gagcttgacc ccaaatacgt ggacgtgatt gtcgaacgct
    12421 ggcaagaagc cacaggaaaa acggccatcc acgctgatac ggggaaggca ttcaaaatga
    12481 atgattaaca atatctttat atcaaacttt acgggtgaga agactgcaat gctctgcttt
    12541 tggctttaga gcttctccac atctttcgca attgacgata aatttcagtc gctgtctttt
    12601 ttgtttcggg taagccagat tgccgcaatg aaaagtcctt tggcgcgcgg cagaataaga
    12661 aagacgcaaa gcaaagctat tataatcagt atagttgttt ctacaacgtc cggaaaaaca
    12721 tcatgtgtta caaaataaaa tataaacggt gccatgatgt ggcccgtaat aagaattgtc
    12781 agccacggcg gtgcatcatc agcgcggatg tctgagaaat cttcatgaca aacagagcaa
    12841 attttgtttt gtgttatata tgatttaaaa agattccctt gtccgcaatt cgggcaatcc
    12901 ccttttatgc cgcgccagag cacgtcctgg gttttgtttg tcttgttttt gttcattttc
    12961 atatgcgcca aatgatgttt tatacatgat atttatatct tgaaacattc ttttataatg
    13021 cttatatatg atttatgaag tcaaatacac agcctaaaat tatcaaagat gatcttccaa
    13081 tcaaaaaacc gcctcaaaaa cagtggatcg ataagcgtgc agccaatcaa aaagagccaa
    13141 aatacttact tcccaaagag gaagattgca gcaacagagc tacggattga gtcatgatta
    13201 cgtcaccaca acttcgcgcc gcccgcgcct tgttgggctg ggatcaaaaa caactagctg
    13261 agctttccgg cctttcactg ccaacgatcc agcgtatgga gagcagtgat gatatggtac
    13321 gtggcaatgt cgattctctt acaaaaataa taagcgccct aaatgaagct ggggttgagt
    13381 tgatcggtga atccgtcatc agcaccggcc aaggccgggg cgttcgcctt atcaataaat
    13441 ccgaagcgtc ttgaatatga ttcctgcaca ttttacaccc aagctttttt cacttctccg
    13501 tcagggctat aattttgata cgtttaaatc agatgttatg gctgggctaa ccgtagctat
    13561 tgtggctctg cccttgtcga tggcattggg aattgccagc ggcgcatcgc cggataaggg
    13621 actaattact gccgtcattg ccggattttt aatctcgttt cttggcgggt cgcgggttca
    13681 gattggtggg ccgactggcg cgttcattgt tgttatcttt aacgtgattg cccagtacgg
    13741 ctatgatggc ttgattctgg ccacgcttct ggccggaatt atccttgtca ttgccgggta
    13801 tgcaaaattt ggacgggtcg tcaaatatat tccctatcct gttgtcacag gctttaccgc
    13861 cggaatagcg gtcattatag cctcctcaca gatcaaggat tttctcggtc tgcatgttcc
    13921 cgaagtcccc gcagaattta ttgccaaatg gcaatcctat tttttgcata ttgatacatt
    13981 tagcctgata accctcatcg ttgggttggc agcattgcta acgatcatct tgttaaaacg
    14041 ctttgcaccc aagttaccgg gctatcttat cgccgtggcg ataacggcac tcaccgtttt
    14101 tgtttttcac ttgccggttg agacaatcgg ctcacggttt cccgatatgc cttcggggct
    14161 gccaatgccc catatcccag cttgggattt tgaaaaattt attagcatcg ttccgtctgc
    14221 ttttaccatt gcctttctgg caggcgtgga atctttgctc tctgccgtag tagcggacgg
    14281 tatgacagga tataagcatc gctccaatca ggagcttgtc gggcaaggtg ttgccaatat
    14341 cgcctcagcc ttcttcggtg gaatgcccgc gactggggcc atagcccgca cggctacaaa
    14401 cataaaagca ggtggaaaaa ctccaatggc agggatgttt catgccttat tcatcctgct
    14461 gttcatcctg tttgcaaccg atatcatggc ttttgtgccg cttgccgcgc tatcggcgat
    14521 tttatttatc gtggcctgga atatgagcga atttcaacat tttatccata tcttcaggct
    14581 ctcaaaaagc gaccgtactg ttatgcttct aacttttgcc ctgacggttc tggttgattt
    14641 gactgtggct attggtgtcg gcgtgaccct agcttcctta ttgtttatgc gtgagatgag
    14701 caaatccgtt gtactgcgtt ctcagggacg tcatatacgc gatatcttta acgataacga
    14761 agaggatgta gagcaaaggg atgatctccc tgtcggcgtt gaggtttttc gcatcaccgg
    14821 accactcttt tttggtgttg ccggaacgat gctcgaaacc ctccaatcta tgggcgaagt
    14881 gccaaaaatt cttattatcc gcatgaaact ggtaccgtat atggatgtgg caggcttgaa
    14941 tgccatgaga aatcttatga agggatgcca taccaaacac acaaaagtga tcttgagcgg
    15001 tttgcagcct caacctgggg atatgctgga caaggcggat ataaaagaca atcacacaaa
    15061 tttatggata tcggccagtt acgatgatgc cattgctctt gcgcaccagc ttgtcgcgga
    15121 acctacggtt tcaatgtaga ttgatgtttt atgtctctct ttttttgatt acgtaacttt
    15181 cttttcttca gattctgtat caagtttttc gattttatag gcagaggtgg ccccaagaat
    15241 tcggacagtg agttaagcta cgatctatga ccgaa
//
LOCUS       DAOSWR010000040        15217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2447_length_15217_cov_5.151761, whole genome shotgun sequence.
ACCESSION   DAOSWR010000040 DAOSWR010000000
VERSION     DAOSWR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_15217_cov_5.151761"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..2223)
                     /locus_tag="PK513_04515"
     CDS             complement(73..2223)
                     /locus_tag="PK513_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81744.1"
                     /translation="MNETRFPVGVVPPGQSSRAASASEPNARLIGVPNEVRAELQTRK
                     AALRLRGEVVRHNDDGSVRVRTPRGDVDVQLREGQPRPERGARVELEIQPPKTPDRPP
                     ENVTVRTLPHAPVRGESEAPAPPAASPPPARASATPVAVHVRGESQAPAPPRPPLPAD
                     VRHGQNAGEKVVQSLPPEGSVVRLQPLPARALQSLPALPDLIQQAVQVVIQPAVFQTH
                     ILARQAETEISQAVLNIQRPATLPAPEVFETLAPLTQPVPEVSARPLPLTQPTQNTPL
                     PAAITAPQSFPIPQAQVFSQNIALVVPETDSAPALTPAPVQTRVLSIPFSPAPRIALP
                     LPIADFVSSPLPVSTITAPTRVQPLQPLAPVLVKPQALDVVIAKILPPDVQILSGSLG
                     VETPAPGTLQVTKPEMLIMHNQNAGNLVGVVTAVTPDTLPVISVFFQQLGNEQLFTLQ
                     FPSESVTIGTQIQVTPHVSPSSAQMLVTPPALPQEIPLSAFLTPQPWPALDEALQLLA
                     RAVPQTAQAMVNVTPSPGAPAQLGPAIMFFVAALRGGDLGSWLGEKATSILKSEKGGH
                     SLSRLLSDGAVMARVAREPMGQDWRALNIPLYYEGDMHKIGLYYKHEHDEQEDGLSPL
                     KGTRFVFDLSLDAMGKVQLDGLFRPVSDMGKRLDLVVRTEDNFSEATRAEMRRVYARA
                     LRDSGVTGELLFQTQPESWVTVSTNQDNVLGVSA"
     gene            complement(2307..3137)
                     /locus_tag="PK513_04520"
     CDS             complement(2307..3137)
                     /locus_tag="PK513_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOO81745.1"
                     /translation="MSDTQTHVEARTKAPSFRRGKNLLAVASGKGGVGKTWFSVTLAQ
                     ALTQMGKKVLLFDGDLGLANVDVQLGLMPKRDLNDVIRGRLSLDKVIQHYDEGGFDII
                     AGRSGQASLSSLPSQRLAHLRDQLLEVADNYDIVIADLGAGVDRTVRMLSATAMRTIL
                     ITTDEPTSLTDAYAFIKLGNAAGMSKNISIVVNQAPSEMEGEKTYKTLLKACSNFLRL
                     QPPLAGLVRHDPKVKETIRHQTPILIRSPNAQAAGDVEAIARHVWKSMSEARKAAADG
                     "
     gene            complement(3134..4243)
                     /locus_tag="PK513_04525"
     CDS             complement(3134..4243)
                     /locus_tag="PK513_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HOO81746.1"
                     /translation="MRLKSFYAKTMTEAMHMVREALGEDAIILATREERGGPSRGGAV
                     RVTAAIDPGDYDERAFDDTGFGTGDLGGPNFETHGRSAPPSADTWLQYDEEDEESAVA
                     EEITDAMLRHAVPEDVMDQILSCATVVGFEQPGVALVAAMEHLFQFTPLPQGVTSKAL
                     MFVGPPGSGKTLAAAKAAARGVMNGLKVGVISTDTVRAGGLEQLAAFTKLLRTDLKAA
                     ESPEALAKAVAGFKGYDQIIIDTAGMNPFNTDDIRKLARLIGAAPIEPYLVLPGGMDA
                     EECGEIGRVFSTIGAHTLVPSRVDMARRLGGLLSAAHHGGLSFADASNTPKVADGFVS
                     LNPKNLSKLLMPGAYRNETARGQHQPALRRTGTHQ"
     gene            4440..5147
                     /locus_tag="PK513_04530"
     CDS             4440..5147
                     /locus_tag="PK513_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81747.1"
                     /translation="MLDTPTKFTVDASDIIEQLPHLRPRLLDLFGKVSRVYSRSDVRS
                     HLAVMPNMYYILSSGHFAGDGKKYTEVGKLNRKFDFSSSSSALRARTKQDIGERYTEL
                     ELGSALYYERLVSISANLKVVKGKGGLETTLEGFNLSEIGLREPQTAQQAQAVLDAMS
                     SALESLKSGKHVEDRIFSLARLRKFLISEGIEPVEGFPVKRLEDFDAELLEKVSQGVA
                     RRVAPSQTPPPSSLEVI"
     gene            complement(5361..7448)
                     /gene="flhA"
                     /locus_tag="PK513_04535"
     CDS             complement(5361..7448)
                     /gene="flhA"
                     /locus_tag="PK513_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO81748.1"
                     /translation="MNSAAGALAPYFRSDVLFAFGIIGIIMFMLVPMPTFMLDVGLSI
                     SIMFSVMILMTVLFIRAPLEFSTFPTVLLVATSLRLGLNVASTRLILSEGHTGSDAAG
                     QVIGAFGTFIIQDSYVVGAIVFAILVIVNFVVITKGSGRIAEVAARFTLDAMPGKQMA
                     IDADLSAGLITEDEAKERRSKLEQESTFFGAMDGAAKFVRGDAIAGLLITFINIVGGI
                     VIGTVSEGLSLGEAASTYTILTIGDGLVSQIPALVVSVGAGLLVSKAGVAGSAEKVLF
                     EQFGAYPRALAMSAVLMLILGLVPAIPAAPFVTLAAVTGVMSYFSFQRLAVAEEAEKA
                     ATAAAMRPGAPGSPVPAEEPISKALAMDTIRLEMGYGLLPLVQGDGGNKLTDQIKGLR
                     RQLAEDMGYVLPAVRIQDNLQLPANTYVVRVKEIEAGRGEVRPNMNMCMDPTGAKITL
                     PGEATVEPTFGLPAMWIDEQYKEEAHFKGYTVVDAPTVITTHITEIIKDNMPDLLNYA
                     ETQKLLDELPKEYQKLVADVIPSQINVGGLQRVLQNLLGERVSVRDLPAILEGIAEAS
                     AFTKNIAHITEHVRSRLARQLCDSNTNASGVVALVTLTPEWEHAFGDALMGDGEEKQL
                     AMPPSKLQDFITVIRERYDELAMQGETPVLLTSPAIRPYVRSVIERFRPQTVVMSQNE
                     IHPKARIKTLGQV"
     gene            complement(7686..8507)
                     /locus_tag="PK513_04540"
     CDS             complement(7686..8507)
                     /locus_tag="PK513_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81749.1"
                     /translation="MMSEEDSKLEQLGAAAKNAGQAVVETLKDDEKRGAIVQTVVDGA
                     ESTVDKLTDTLNNAALGIKSLTKDDLTAENIAKIAQEKGPDAVVEAIKAAGYFSLFKG
                     PTQIAAMLAGDNGASKLADVINGKVKPDGKPADRPTESLDLMAMIMSGDFSGLKSHAE
                     SWFDDITEKLSGGFGQMISKFTGFDLSGITGMLAGLKNTVMGFFGSMGGGEDAPEETM
                     VADTSVYTTPQVEKTSGAGLDGVPSLTDEHKVVSNTPAAKPATPALEGPSGPTNG"
     gene            8692..9615
                     /locus_tag="PK513_04545"
     CDS             8692..9615
                     /locus_tag="PK513_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO81750.1"
                     /translation="MEIFVIVFVGLCILLVFKGIKSVPQGYEFTIERFGKYIHTMEPG
                     LHFIVPFVDAIGAKMNMMETVLDVPSQEVITKDNALVQVDGVVFYQVLNAARAAYEVL
                     DMERATLNLTMTNIRTVMGSMDLDELLSERDAINARLLSIVDEATEPWGIKVTRIEIK
                     DITPPRDLVDSMARQMKAERDKRATILEAQGFRQAEIERAEGEKQAAILEAEGRREAA
                     FRDAEAREREAEAEANATRVVSEAIAKGDPRAINYFVAQDYVGALGKFANSPNQKILM
                     MPLEASAVIGALGGVAEISKELFSKDDKKVA"
     gene            9619..10086
                     /locus_tag="PK513_04550"
     CDS             9619..10086
                     /locus_tag="PK513_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO81751.1"
                     /translation="MELFVYNLEEIVYWHWLVLAGVFFILEILSMSFFFLWIGVSAVI
                     MGVLVLIAPEVSWQLQFSIWAALSVIGAVGWRVYKKNNPNAIKSDQPNLNKRGDQYVG
                     RTFTLAEPITNGFGKVKVDDSIWKVECKTDLEAGAKVKVAAVDGTVLQVEAVE"
     gene            complement(10496..11911)
                     /locus_tag="PK513_04555"
     CDS             complement(10496..11911)
                     /locus_tag="PK513_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO81752.1"
                     /translation="MRLMIIGQLEGYISQAGKIALQKGAKVIHCEDTEQAIGALLNGK
                     GADLAMVDVKQKIRSFIDRLEAERIHMPVIACGIGTDARAAVKAIEQGAKEYVPLPPD
                     ADLIAAVLAAVSEEQRSMIADDPSMRGVMTLADKIAPSEATVLITGESGTGKEVMSQY
                     IHGKSKRASGPFIAVNCAAIPENLLESELFGHEKGAFTGATGRRIGKFEEAHAGTLLL
                     DEVSEMHPSLQAKLLRAIQEREITRVGSNDAVKVDVRILATSNRDLEKSVEKGEFRED
                     LYFRLNVVNIALPALRERPKDIVTLAQFFADKYAQANGVEKKVLSAEAKAKLQTYGWR
                     GNIRELENTMHRAILMAVEDELEADAIHLADGAFQTGTPAQTAASALDPAGVGGAGAK
                     PVAAAQGAGPVENPGGVDTLVGRTIADVERDMIINTLEHCLGNRTHAANILGISIRTL
                     RNKLNAYKDEGVAVPTSASGG"
     gene            complement(12000..12824)
                     /locus_tag="PK513_04560"
     CDS             complement(12000..12824)
                     /locus_tag="PK513_04560"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO81753.1"
                     /translation="MKTERIAGLRKVLFTLGVLYFALIAVLFVKQRDFIYIPDKTVPS
                     KSLVSADGMAQDIEVHTEDGLFLKAWFIPSVQRGMPVILFFHGNAGNISHRLFKVSDY
                     IEAGYGVLMAEYRGYGGNPSTPSEEGLYADARAYFSWLNAHGYKDEDLILYGESLGSG
                     VAIEMAARRKVKALILEAPYARLSDPARKTYFFIPFIEHLMHDKFLSIEKIHAVNAPK
                     LFLLAGKDEVLGAQTGMELYEAAHEPKTLKIFENASHNGAYQYGAGSLVLLFLSTL"
     gene            complement(12856..13707)
                     /locus_tag="PK513_04565"
     CDS             complement(12856..13707)
                     /locus_tag="PK513_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOO81754.1"
                     /translation="MSDAASQDGKFSDSGPMPVVSVPRHTSSVDFATILGLFFTFGLI
                     IAAIAIGDSNANFIEVRSLMIVLLGTLTATAISYTPEELSRSGAIFSRSVFIHKWNPT
                     AMAANLVSLAVIAKKKGVLALTSYESELRKDAFLYRSFQMVVDGFPADDIEFLLTQEL
                     EAGIERHKRSASITRRASEIAPAMGLIGTLIGLVMMLADLENPETIGPAMAVALLTTF
                     YGAILGTIVMAPLAVKLEKRSSDESLIKRLIKIAAVSVARQENPRKLEMLLNSELPPA
                     KRIRYFD"
     gene            complement(13724..13972)
                     /gene="fliN"
                     /locus_tag="PK513_04570"
     CDS             complement(13724..13972)
                     /gene="fliN"
                     /locus_tag="PK513_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOO81755.1"
                     /translation="MADDVTAIYEIPVQISAVLGRSTMQVSQLLKLGRGAVVELDRKV
                     GEAIDIYVNNRLVARGEVVVVEDKLGVTMTEIVKSDRA"
     gene            complement(14133..14918)
                     /locus_tag="PK513_04575"
     CDS             complement(14133..14918)
                     /locus_tag="PK513_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81756.1"
                     /translation="MRTKTVEDIKQKFFFDHNIFDANGNIESELLEEEEPPIPVFSEA
                     ELEAAKKAAFQNGHEQAMQEAKASRAQHLAGVLGALAKDMTSLFAQEDARERLYEREV
                     VALVSQIYEKLFPHYAEKAGFGELTAALGNVLGNHSRQSLVLVRIHPDVSDGVEKFLQ
                     KLQSQNPELRYTVQGDETLSAYSCSLSWEDGGALHNNEALAEEILGILKDGSKDGLAG
                     GAAKRHDGRKGGGDETAPQAALSETEERNENQEHPDLKERPDE"
     gene            complement(14931..>15217)
                     /locus_tag="PK513_04580"
     CDS             complement(14931..>15217)
                     /locus_tag="PK513_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOO81757.1"
                     /translation="NLDPASVQTLIRAVDKDKLPTALKGATETIKDLFFSNMSERAAK
                     IMKEDMAAMGPVRLKDVEESQQYVVNVAKDLESRGEITIASGDAEDELIY"
BASE COUNT         3755 a         4331 c         3613 g         3518 t
ORIGIN      
        1 gataggccga cgcggcaatc cagaactgga ttgcttcgct ctcgttggtc gcgcacaatg
       61 acacggtaca aattaggcgc tcacgcccaa cacattgtcc tgatttgtcg aaaccgtaac
      121 ccagctttcc ggttgtgtct ggaacaacaa ttcacctgta accccgctat cgcgcaaagc
      181 gcgtgcatac acccgccgca tctcggcgcg tgtcgcttcg ctaaaattgt cttccgtgcg
      241 caccaccaga tccaaacgct tacccatatc cgacactgga cgaaacaacc cgtcgagctg
      301 aactttgccc atggcatcca aagacaggtc aaaaacaaac cgtgtgcctt tcagaggcga
      361 gagcccatcc tcttgttcgt catgctcatg cttataataa agcccgattt tatgcatatc
      421 gccctcgtaa tacaaaggga tattcagcgc ccgccaatct tgccccatcg gctcacgcgc
      481 cacgcgggcc attaccgcac catcgctgag caggcggctt aaactgtgcc cgcctttctc
      541 gcttttaaga atgctggtcg ctttctcgcc aagccacgat cccaaatcac cgccgcgtaa
      601 ggctgccaca aaaaacataa tcgccgggcc aagctgtgcc ggagcgccgg gtgaaggggt
      661 cacattcacc attgcctgag cagtttgtgg caccgcgcgg gccaaaagct gcaaggcctc
      721 atccagcgct ggccagggct gcggcgtaag aaaagcagac aacggaattt cttggggcag
      781 ggctggcggc gtgacaagca tttgcgcact tgatggtgaa acatgcggtg tgacctgaat
      841 ctgcgtaccg atcgtgacgc tctcggacgg gaattgcagc gtgaaaagct gttcatttcc
      901 caattgctga aaaaacacac tgatcaccgg caatgtatca ggcgtcaccg ctgtcacaac
      961 gcccacaaga tttccggcat tttgattgtg catgatcagc atctcaggct ttgttacttg
     1021 caatgtgccc ggcgcaggcg tttcaactcc aaggctacct gaaaggatct gcacatccgg
     1081 cggtaagatt ttcgcaatca cgacatccaa agcctggggc ttcacgagca ctggcgcaag
     1141 cggctgtagt ggctgcacac gtgttggcgc agtgatagtc gaaacgggaa gaggcgaaga
     1201 tacaaaatcc gctattggca gaggcaaagc aatcctaggc gccggggaaa agggaatgct
     1261 caaaacacgc gtctgtacag gagcgggagt cagggcaggg gcgctatctg tctcgggaac
     1321 aacaagcgcg atattttgag aaaacacttg cgcttgcggt ataggaaaac tttgtggcgc
     1381 tgttatggca gcaggtaaag gcgtgttttg tgttggctgc gttaatggta atggcctcgc
     1441 tgatacttct ggtactggct gcgtaagtgg tgcaagcgtt tcaaaaacct ctggcgcagg
     1501 tagggttgct ggacgctgaa tattcaggac cgcctgcgag atctctgttt ctgcctgacg
     1561 ggcaagtata tgcgtttgaa aaacggctgg ctgaattact acttgtactg cctgctgaat
     1621 cagatcgggc aaagccggaa ggctttgcaa tgcccgcgca ggaagcggtt gtaagcgcac
     1681 gaccgatcct tccggtggta ggctttgcac caccttttca cccgcatttt gcccgtgacg
     1741 gacatcggcg ggtaaaggcg ggcggggcgg cgcaggcgct tggctttctc cgcgcacatg
     1801 tacagccacc ggcgtggcgc tggcacgcgc cggtggaggg gaggcggcag gcggcgcagg
     1861 cgcttcgctt tccccgcgca ctggtgcgtg cggtaatgtt ctcacagtta cattttcagg
     1921 cggacgatcg ggagtcttgg gcggctgaat ttcaagctca acgcgggctc cgcgttccgg
     1981 ccttggttgt ccttcgcgaa gctgtacatc cacatctccg cgcggtgtgc gcacgcgcac
     2041 cgagccgtca tcattatgcc gcaccacttc accacgcagg cgcaaagccg ccttgcgcgt
     2101 ctgcaattcg gcgcgcacct cgttcggcac gccaatcagc cgggcatttg gctcggacgc
     2161 cgaagccgcc cgcgaagatt gccccggtgg cactacgcca actggaaatc gcgtttcgtt
     2221 cataatggaa aacagaatac tgaaatgctg aaatattgga agctgaatga ttggagtaca
     2281 aacttttcca acattccaac cttcaatcac ccatctgccg ccgccttgcg ggcctcgctc
     2341 atcgatttcc acacatgccg cgcaatggct tcaacatctc cagcagcctg cgcattgggt
     2401 gagcggatca ggatcggcgt ctggtgacgg atggtttcct tgactttcgg gtcatggcgt
     2461 accagtccgg ccaagggcgg ctgcaggcgc aggaagttcg aacaggcctt gagcagagtc
     2521 ttataagtct tctcgccttc catctcgctg ggagcctggt tgaccacgat ggaaatattc
     2581 ttcgacatcc ccgccgcatt gcccagcttg ataaacgcat aagcatcggt tagcgaggtc
     2641 ggctcatccg tcgtaatcaa aatcgtgcgc atcgccgtgg ccgaaagcat ccgcacggtg
     2701 cgatcgacgc ccgcgccaag gtccgcaatc acaatgtcgt aattatcggc cacttccagc
     2761 aactgatcgc gcaaatgcgc caaccgttgc gagggcaggg aggacagcga ggcctgcccg
     2821 ctgcgcccgg cgataatatc aaatccgcct tcatcataat gctggatcac cttatcaagg
     2881 ctcagccgcc cgcgaatcac atcgttcaga tcgcgcttgg gcatcaggcc gagctgcaca
     2941 tcgacattcg ccagccccag atcaccgtca aacagcaaga ctttcttgcc catttgcgtg
     3001 agggcttggg ccagcgtgac agagaaccac gtcttgccaa ccccgccttt accgctggct
     3061 acggccagca ggttcttccc gcggcgaaag ctcggggcct tagttctggc ttcgacgtgt
     3121 gtttgcgtgt cactcattga tgtgttcctg ttcggcgaag cgccggttga tgttgaccac
     3181 gcgcggtctc attacgatac gcgccgggca ttaacagttt agacaaattt ttcggattaa
     3241 gcgagacgaa accatcggca actttcggag tattcgaggc atcggcaaag ctaagtccgc
     3301 catgatgcgc tgcagacagc aagccgccga gcctgcgcgc catatccacg cgggaaggca
     3361 ccagcgtatg cgcaccgatc gtggaaaata cgcggccaat ctcgccgcac tcttccgcat
     3421 ccatcccgcc gggcaaaacg agataaggct caatcggagc cgcgccgatc aaccgcgcca
     3481 gcttccgaat atcatcggtg ttgaacggat tcattccggc ggtatcgata atgatttggt
     3541 catacccttt aaaccctgcc actgctttgg ctaaagcctc agggctttcc gctgccttca
     3601 aatccgtgcg cagcagcttt gtgaaagcgg ccaactgttc aagcccgcca gcccgcaccg
     3661 tatcggttga aatcacgccg accttcagcc cgttcatcac gccgcgcgcc gcagcctttg
     3721 ccgcagccaa agtcttcccc gaacccggcg gaccgacaaa catcaacgct ttgctggtta
     3781 caccttgagg caggggagta aactgaaaca aatgctccat cgccgcgacc agcgccacac
     3841 cgggttgttc aaacccgacc acggtggcgc aggagagaat ctggtccatt acatcttcgg
     3901 gcaccgcatg gcggagcatc gcatcggtaa tttcttcggc caccgccgat tcttcatcct
     3961 cctcatcata ttgcaaccac gtatcggcgg aaggaggagc gctccgccca tgtgtttcaa
     4021 aattcggccc gccaagatcg ccagtgccaa agcctgtatc atcaaacgcc cgttcgtcat
     4081 aatcgccggg gtcaatcgcc gcggtcacgc gcaccgcgcc cccgcgtgaa ggcccgccgc
     4141 gctcctcgcg cgtcgccaaa atgattgcat cttcgcccaa agcctcacgg accatatgca
     4201 tcgcctctgt catggttttg gcataaaagg atttcagccg catgtgtgta tatcttactc
     4261 aagcaaatta taaacgcaac ttagtgtagc acaccaccat cttccataaa aagcgggata
     4321 tgcctgtgga tgagtagggg ataaataaag gctttttgag aacattgttg acataataac
     4381 aaaatttaac tatattctta ctatattcaa aatatgcgtt taaaaggata aagggctccg
     4441 tgctagatac gccaacaaaa ttcacagttg atgcgagcga tataattgaa caactcccgc
     4501 atttacgtcc gcgtttgctt gacttgttcg gcaaagtttc gcgtgtctac agcagatcag
     4561 atgtaagaag tcatttagct gtaatgccta atatgtatta catactttca agtggtcatt
     4621 ttgccggaga tggaaaaaaa tacactgaag ttggaaagct taaccgaaaa tttgattttt
     4681 catcctctag ctctgccctg agagcgagaa caaaacaaga tattggcgag cgttatactg
     4741 aattggaatt gggtagtgcg ttatactacg agagattagt cagtatatca gcgaacttaa
     4801 aagttgttaa aggcaagggt ggccttgaaa ccactttgga aggatttaat ctgtccgaaa
     4861 tcggtttgcg ggagccgcaa acagcgcaac aagcgcaggc agttctggat gcgatgagtt
     4921 cagctctaga aagcctgaaa agtggaaagc atgttgaaga cagaattttt agtttagcta
     4981 ggttaagaaa gtttctaatc tcagaaggta ttgagccggt tgaaggtttc ccggtaaaac
     5041 gtttagagga tttcgacgca gagcttctag aaaaggtcag ccagggcgtt gcccgaagag
     5101 tggcgccctc ccaaactcct ccgccttctt cgcttgaagt catttaatta aaaaacatcc
     5161 ccatacattt gttcgcctta aacctgcccc agcgtcttaa ttcgcgtttc ctatcgtcat
     5221 tgcgaggagg ccgctaggcc aacgcggcaa tccatcttat gcggatagtt tcagagatgg
     5281 attgcttcgt cgcttacgct cctcgcaatg acgactttct aaaaaaactc tgcgccctct
     5341 gcgtctctgc ggttaaaaaa ttaaacctgc cccagcgtct taattcgcgc tttcgggtga
     5401 atttcgttct ggctcatcac caccgtctgc gggcggaaac gctcaatcac ggaccgcaca
     5461 taagggcgga tcgcgggcga ggtcaacagc accggcgttt cgccctgcat ggccaactcg
     5521 tcataacgct cgcggatcac ggtgataaaa tcctgcaact tcgacggcgg catggcaagt
     5581 tgcttttctt cgccatcgcc catcagcgca tcgccgaacg catgctccca ttccggtgtc
     5641 agggtgacca gcgccaccac gcccgaagca ttcgtgttgg aatcacacag ctggcgagcc
     5701 aggcgggagc gcacatgctc ggtgatatgc gcgatatttt tggtaaaggc gctggcctct
     5761 gcgattccct ccaaaatcgc cggcaaatcg cgcaccgaaa cccgctcgcc cagcaaattc
     5821 tgtaacacgc gctgcagtcc gccgacatta atttgcgagg gaataacatc cgctaccagc
     5881 ttctgatact ctttgggcaa ttcatcgagc aatttctgcg tctcggcgta attcagcaga
     5941 tccggcatat tgtctttaat aatctcggta atatgcgtgg tgatcaccgt cggcgcatcg
     6001 actaccgtat agcctttaaa atgcgcctct tctttatatt gctcatcaat ccacatcgcg
     6061 ggcaaaccaa aggttggctc cacagtcgcc tcaccgggca gggtaatttt cgcccccgtc
     6121 gggtccatgc acatattcat attgggccgc acctcgccgc gcccggcttc gatctctttg
     6181 acccgcacaa catacgtatt ggccggaagc tgcaagttat cctgaatgcg aaccgccgga
     6241 agcacataac ccatatcctc ggcaagctgt cggcgtagcc ctttgatctg atcggtcagc
     6301 ttgttgcccc catccccttg caccagcggc aagaggccat agcccatttc caggcggatc
     6361 gtatccatcg ccagcgcttt ggaaatcggc tcttccgccg gaacggggga accgggcgcg
     6421 ccgggacgca tcgcagcagc agtggcggct ttttctgctt cctctgctac tgccagccgt
     6481 tgaaaggaga aatacgacat cacgccagtg acagcggcca aagtcacaaa cggcgccgcc
     6541 ggaattgccg gaaccagccc caaaatcaac atcagcaccg cactcatcgc caaagcccgc
     6601 ggatatgcgc caaattgctc gaataaaacc ttttctgcgg accccgcaac cccggccttg
     6661 gaaaccagca gaccagcgcc gacagacaca accagcgccg gaatctggct gaccagcccg
     6721 tcaccaatgg tcaatattgt ataggtcgaa gcggcttcac ccagcgacag cccttctgaa
     6781 acagtgccga tgacaatccc gccgacaatg ttgataaagg taatcagcaa acccgcaatc
     6841 gcgtctccgc gcacgaactt ggccgcaccg tccatcgccc cgaaaaaggt gctttcctgc
     6901 tccagctttg atcgccgctc cttggcttcg tcttcggtaa taagcccggc ggataaatcc
     6961 gcatcaatcg ccatctgctt gcccggcata gcatccaggg taaagcgtgc cgcgacttcg
     7021 gcgattcgcc ccgaaccctt ggtaatcacg acaaagttca caatcaccag aatggcaaac
     7081 acaatcgcgc ccaccacata gctgtcctga ataataaacg tcccgaatgc gccaatcact
     7141 tgccctgccg catccgatcc cgtatgtcct tccgataaga tcagccgcgt cgaggccacg
     7201 ttcagcccaa gtctgagcga agttgccacc agcaagacgg tgggaaatgt cgaaaattcc
     7261 agcggcgcgc ggataaacag caccgtcatc aaaatcatca ccgaaaacat gatcgaaatc
     7321 gacaggccaa catcgagcat aaaagtcggc atcggcacca gcatgaacat gatgattccg
     7381 ataatcccga aggcgaacag aacgtcgctg cggaaatacg gtgccaaagc gcccgccgcg
     7441 ctattcatat tcataccgcc acccatgcgc accggcgcag ccggagaagc ggctttctga
     7501 gcgggaatat tggaaccgga tgaatcagcc atgacttacg ttctgcaaat aaccttgtga
     7561 tgtcattcct gcgaaagcag gaatctatga aacgcaacaa cacaccccga ttattatcag
     7621 ggcacatatc gccattctat ctgcgtgatg aaattaaggc aaatgaaagg agttaaagag
     7681 gttggttatc catttgtagg cccgcttggg ccttctagtg ctggagttgc tggtttggct
     7741 gcaggcgtat tggagacaac tttatgttca tctgttagac tcggcacgcc atcaagcccc
     7801 gcgccggaag ttttttcaac ttgcggcgtt gtatatactg aagtatctgc aaccatggtt
     7861 tcttccggcg catcctcccc gccgcccata ctgccaaaga atcccatgac cgtattttta
     7921 agacccgcaa gcatgcccgt gatgccggaa agatcaaagc cggtgaattt tgaaatcatc
     7981 tggccaaagc caccacttag tttttcagta atgtcatcaa accagctttc agcatgactt
     8041 ttaaggccgc tgaaatcgcc gctcataatc atcgccataa gatcaagact ttccgtaggc
     8101 ctatcagcag gttttccatc gggcttaacc ttgccattga taacatcggc cagtttgctc
     8161 gccccgttat cgccagcgag catggcggca atctgtgttg gacctttaaa gagagaaaaa
     8221 tatccagcag ctttgatcgc ttcaacgacc gcatccggac ctttctcttg cgcgattttt
     8281 gcgatgttct cggctgttag atcatctttt gtcagagatt tgatgcctag ggccgcatta
     8341 ttcaatgtgt cggttaattt atcgacggtc gactctgcgc cgtcaacaac ggtttgaacg
     8401 atcgcccctc gcttttcatc atctttgagt gtctcaacaa cggcctgacc ggcatttttc
     8461 gccgccgcac cgagctgttc aagcttgctg tcttcttcag acatcattca cacctttatt
     8521 attctctatt atatggccgt ttttttggac cttttttcgt gttcccatta tgaagtccaa
     8581 accttaataa aaccttaata ctagcccaaa agcctgctta acccttgcgt gccgttgcca
     8641 aatgcgccat gcttcttgta aaatttgaaa tcaaaacaag gaatcacatc catggaaatt
     8701 tttgtcattg tctttgtcgg cctgtgtatc ttgctggtat tcaaggggat caaatccgtc
     8761 cctcaaggct atgaatttac catcgaacgc ttcggcaaat acatccacac catggagccc
     8821 ggcctgcatt tcatcgtgcc cttcgtcgat gccatcggcg caaaaatgaa catgatggaa
     8881 accgttcttg atgtcccctc gcaggaagtc atcaccaaag acaatgcgct ggttcaggtt
     8941 gacggggttg tattctatca agttttgaac gccgcgcgcg ccgcttacga agtgctcgat
     9001 atggagcgcg caacgctcaa cctgaccatg accaatatcc gcacggtgat gggctctatg
     9061 gatctcgacg aactcctctc cgagcgtgat gcgatcaacg cccgcctgct gtccatcgtt
     9121 gatgaagcga ccgagccgtg ggggatcaaa gtgacccgga ttgaaatcaa agatattacc
     9181 ccgccgcgcg atctggttga ttccatggcc cgccagatga aggccgaacg cgacaaacgc
     9241 gccaccattc tggaagccca gggcttccgt caggccgaaa tcgagcgggc cgaaggggaa
     9301 aagcaagccg caattttgga agccgaaggc cgccgtgaag ccgccttccg agacgccgaa
     9361 gcccgcgaac gtgaagccga ggccgaagcc aacgccacgc gcgtggtctc tgaagccatc
     9421 gccaaaggcg atccgcgcgc catcaactac ttcgtcgcgc aggactatgt cggcgcgctc
     9481 ggtaaattcg ccaattcgcc gaaccagaaa attctgatga tgccgctcga agcctccgcc
     9541 gtcatcggtg ctctgggtgg cgtcgccgaa atctccaaag aactcttcag taaagatgat
     9601 aaaaaggtag cgtaacccat ggaattattt gtctataacc tcgaagaaat cgtttattgg
     9661 cactggctcg tgctggccgg ggtgttcttt atcctcgaaa ttctgtcgat gagcttcttc
     9721 tttctgtgga tcggcgtctc cgccgttatc atgggcgttt tggtactcat cgccccggag
     9781 gtatcctggc aattacagtt ctcaatctgg gctgcgctct ctgtgatcgg cgcggtcggc
     9841 tggcgtgttt acaagaaaaa caaccccaac gccatcaaat ccgaccaacc gaacctgaac
     9901 aagcgcggcg atcaatatgt cgggcgcacc ttcactttgg cagaaccaat caccaacggt
     9961 ttcggcaagg tcaaagtcga tgattccatc tggaaagtgg aatgcaaaac cgaccttgaa
    10021 gcaggcgcaa aagtcaaagt cgccgccgtc gacggcacag tgctacaggt cgaggcggta
    10081 gaataggaat atcggcaaga ttatctcttg atagcaagac atcaatccgg ctcgttcaac
    10141 ggctgaattg gagcgtccgt ccgcaccgtt tcaagaaaat tccggcaact cctttgaaat
    10201 tgctcaaaag gtgtcgggcg tacattcggc atatcggggc agcgcgtatc gcaaaaatgg
    10261 tcccgggcag gaggagcgct gctcaaaggt tgttcatgac cacgtatcga aatcacctcc
    10321 tctttatgac catgctatga tcagcgccgc gcacagtcaa ggttatggca atttttatcc
    10381 agagctttgt cagaacatac caggatcaaa gggggagaat gttttttaaa aaagctgtaa
    10441 gcgaataagt agaaaaagcc gagtcaaaac tcaatagaac tccgagtgga acgaattacc
    10501 ccccgctcgc gctggtcggc acagccacac cttcgtcttt ataggcgtta agcttattgc
    10561 gcagcgttct gatcgaaatg cccagaatat tcgccgcatg ggtacggttg cccagacaat
    10621 gctccagcgt gttgataatc atatcacgct caacatcggc gatcgtccgg ccaaccagag
    10681 tgtccacgcc gccaggattt tccaccggcc cagccccttg agccgccgcc accggcttcg
    10741 ctccggcgcc cccaacgccc gccggatcta gcgcggaagc cgccgtctgc gccggagtgc
    10801 cagtctgaaa tgcaccatcg gccagatgaa tagcatcggc ttccagctca tcctccaccg
    10861 ccatcaaaat cgcgcggtgc atggtgtttt ccagctcgcg aatattcccg cgccagccat
    10921 aggtctgcaa cttcgcttta gcttccgccg acaaaacttt tttctcaacg ccattggcct
    10981 gcgcatattt atcggcaaaa aactgcgcca gcgttacgat atctttaggc cgttcccgca
    11041 aagcgggcag ggcgatattc accacattca accggaaata gagatcctca cggaactcgc
    11101 ctttctcaac cgacttttcc aggtcgcggt tcgatgtcgc cagaatgcgc acatctacct
    11161 tcaccgcatc gttcgatccg acgcgggtaa tctcgcgttc ctgaatagcg cgcaggagct
    11221 tggcctgcaa tgaaggatgc atctcggaaa cctcatccag caaaagagtt cctgcatgag
    11281 cctcttcaaa cttaccgatc ctgcgccccg ttgcgccggt aaaggcgcct ttttcatggc
    11341 cgaacagctc gctttccagc aaattttccg ggatcgccgc gcagttcacc gcaatgaacg
    11401 gtcccgaagc gcgcttggac ttgccgtgaa tatactggct catcacctcc ttacccgttc
    11461 cggactcacc ggtgatcaac accgtcgcct cactcggcgc aattttgtcg gccagcgtca
    11521 taacacctcg catcgatgga tcatcggcaa tcatagatct ttgctcttcg ctcaccgctg
    11581 ccagcaccgc cgcgatcaaa tccgcatccg ggggcagggg cacatattcc ttcgcaccct
    11641 gctcaattgc cttcaccgcc gcgcgcgcat ccgtgccaat cccgcaggca atcacaggca
    11701 tatgaatgcg ctcggcctcc agccggtcaa taaaactacg gattttttgc tttacatcga
    11761 ccatcgccag atcagcacct ttaccattca aaagagcacc aatagcttgc tccgtatcct
    11821 cacaatgaat aaccttcgcg cctttttgca gagcgatttt tccagcctga gaaatataac
    11881 cttccagttg tccgatgatc attaaacgca tttaaagtaa actcccgtaa aactcgtaag
    11941 ctataacaat accatgcgcc acagtgtgga caagtaaaag aaggcgaaga cgctatctat
    12001 tataaagtcg aaagaaaaag caaaaccaga gaaccggcgc catattgata cgcaccattg
    12061 tgagaagcat tttcaaaaat tttaagagtt ttaggctcgt gcgctgcttc atataattcc
    12121 atcccggttt gcgctcctaa aacctcatct ttaccagcaa gtaaaaacag cttgggtgcg
    12181 ttcaccgcat gtattttttc aatcgataaa aatttgtcat gcatcagatg ctcaataaac
    12241 ggaatgaaaa agtaagtctt tcttgccggg tccgatagcc gcgcataagg cgcttccaaa
    12301 atcaaagctt ttacttttcg ccgcgcggcc atttcgatag caaccccgct gccaagggac
    12361 tctccgtata gaataaggtc ttcatcctta tatccatgcg cattgagcca tgaaaaataa
    12421 gcccgcgcat cggcgtataa accttcttcg ctcggcgttg atggattgcc gccataaccg
    12481 cgatattccg ccatcaaaac cccatacccg gcctctatat aatccgaaac cttaaacagg
    12541 cgatggctga tgtttccagc attgccatga aaaaacaaaa tcaccggcat gcctctttgt
    12601 accgagggaa tgaaccacgc ctttaagaac aaaccatctt cagtatgaac ttcaatatct
    12661 tgtgccatgc catccgcact cacaagagac ttgctcggta ctgttttatc cgggatataa
    12721 ataaaatcgc gttgtttcac aaacaaaact gcgatcagtg caaaataaag aacaccaaga
    12781 gtaaaaagaa ctttacgcag acctgcaatg cgttcagttt tcaattcttt cctataaagg
    12841 cggagcatta gggccctaat caaaatagcg aatgcgcttg gctggcggaa gttcagagtt
    12901 cagcagcatt tcaagcttgc gggggttttc ctgacgcgct acagacacgg ctgcaatttt
    12961 aatgagtctt ttaattaacg attcatctga tgaacgcttt tctaatttca cggctagcgg
    13021 tgccatgaca atcgtcccca aaatcgcgcc ataaaaagtg gtaaggagcg ccaccgccat
    13081 cgccggccca atcgtttccg ggttttctaa atcggcgagc atcatcacca agccaatcaa
    13141 cgttccaatt aaccccatcg ccggtgcaat ctcggaagcc cggcgcgtta tgctggccga
    13201 gcgcttatgc cgctcaatcc cggcctccag ctcctgcgta agcaaaaact caatatcatc
    13261 agcaggaaaa ccatcaacaa ccatttgaaa agaacgataa agaaacgcat ccttccgaag
    13321 ctcggattca taacttgtca aagccaaaac gccttttttc ttggcaataa cggccaagga
    13381 cacaagattg gcagccattg ccgtggggtt ccacttatgg ataaaaacag agcgcgaaaa
    13441 aattgcccca gaacgcgaaa gctcttccgg agtataagaa attgcagtag ccgtcaaagt
    13501 tccaagtaaa acaatcatca aagagcgcac ttcgatgaaa ttggcgttac tgtcaccaat
    13561 tgcaatagct gcgataataa gaccaaacgt aaaaaacagt cctaaaattg tcgcaaagtc
    13621 tacgctggaa gtatggcgtg gaacactcac gaccggcatt gggccggaat ctgaaaattt
    13681 tccatcttgg gaggctgcgt cactcattca attatcccat taattatgca cgatctgatt
    13741 tgacgatctc agtcatggtt acgcccaact tatcttcaac aacaacgacc tcaccgcgcg
    13801 caacgagacg attatttaca taaatatcaa ttgcttcgcc gactttacga tccagttcaa
    13861 caaccgcgcc acgtccaagc ttaagaagct gactgacttg catggttgaa cggcccaaaa
    13921 cagctgaaat ttgcactgga atttcgtaga tcgccgtgac gtcgtctgcc atcggttcgc
    13981 caatggcata ttcattaccg gtagaaggcg ttgcgccctc agaagcggaa atttcattga
    14041 gattcatccc ttctgccgcc ggtgccgcat caaagatatc gtcaattgcg gactgttcaa
    14101 cgctgccgcc atccgtgtct tgatcgtctt catcattcat cgggtctctc ctttaaatca
    14161 ggatgctcct gattttcatt ccgttcttcc gtctctgaaa gagcggcctg tggcgcagtc
    14221 tcatcaccgc caccctttct accatcatga cgctttgcag ccccacctgc aagtccgtct
    14281 ttcgatccat ctttaagaat gcccagaatt tcctcggcca aagcttcatt gttatgcagc
    14341 gcaccaccat cttcccatga caggctgcac gaataagcgc ttaaggtttc atcgccctgc
    14401 accgtatagc gtaattcagg attttgcgac tgtagttttt gcaaaaactt ttcaacacca
    14461 tcgctaacat caggatgaat gcgcaccaac accagacttt ggcgcgaatg gttgcccaac
    14521 acattcccaa gagcagccgt taactcgcca aaaccggctt tttccgcata atgaggaaaa
    14581 agcttttcat aaatttgtga aacaagcgcc acaacttcac gctcataaag cctttcgcgc
    14641 gcatcttctt gcgcaaacag tgaagtcata tctttggcca gcgcccccaa aacgcctgcc
    14701 aaatgctgtg cacgactggc tttggcttcc tgcatggcct gctcatgccc gttttgaaac
    14761 gctgcctttt tggcagcctc aagttcagcc tcactaaaga ccggaatagg aggctcttct
    14821 tcttcaagaa gttcggattc gatattacca ttggcatcga atatattgtg atcgaaaaag
    14881 aatttttgct taatgtcttc caccgtcttc gtgcgcattt caaatcaaga ttaatagata
    14941 agctcgtctt cggcatcacc gctggcaatt gtgatttcac cgcgtgactc gagatctttc
    15001 gcaacattaa cgacatattg ctgggattct tcaacatctt taagtcgcac aggccccatc
    15061 gcagccatat cttctttcat aatctttgcc gcgcgctctg acatattcga gaagaaaaga
    15121 tccttgatcg tctctgtcgc acctttcaag gccgttggca atttgtcttt atcaacagcg
    15181 cgtataagtg tttgcacaga ggccggatca agattga
//
LOCUS       DAOSWR010000041        15045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2487_length_15045_cov_5.430220, whole genome shotgun sequence.
ACCESSION   DAOSWR010000041 DAOSWR010000000
VERSION     DAOSWR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_15045_cov_5.430220"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="PK513_04585"
     CDS             complement(<1..1706)
                     /locus_tag="PK513_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81758.1"
                     /translation="MRIRSLVSSVAVLGAFLASSALVGDALAQKGSIMAPSTGWAVSK
                     IGDAGGAYCALARKYNQDTVLTLARNEFSETSFALDFQRPILNQQAAMKVILDPGAGQ
                     QRAYEIKPVSDKAFVVRLGRDSSFFDALARTGLLRVETDGQSYHFNVADIDEGQFKLD
                     ACVVNMVMPAAGEDGSLMPDMDAAVVNAGKSFRQEINALRRQITSLEEQNAALKVQMS
                     TRNEGVEETSSSVVQLSGKIRELEDANALLKRQLSAAQTAQAGESLVESDVRALDAKI
                     VTLENENARLQALLQTGEVVQSSHDLERITALEAEVQSLQTRNGNLQASLDAQGEGKE
                     LVDGLRSQIEELENENSQLSTKILAAREAVRSEFKQQIAALNNENAALKTSAGSKVVD
                     AELLEQLRHQIAQVQNENRLLQETAAKAQGDLETQFHEEQEKTVEKVRNEQAVRIAAL
                     ENEVDALKAESESKTKALTEISEGTAALATLRDENAALKEKLAFQEVQRSQSDVMVTR
                     IENLEAENEDLRAQLDVAQQSLSAGSDAAEADANAHVARIADLEAERDALKALAAQGE
                     KKV"
     gene            complement(1885..2964)
                     /locus_tag="PK513_04590"
     CDS             complement(1885..2964)
                     /locus_tag="PK513_04590"
                     /inference="COORDINATES: protein motif:HMM:NF022136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HOO81759.1"
                     /translation="MRTQVCGTVLLMGMVMSGGALANPLTVDPYSSEQVAQSVIQAGF
                     QFLPPLRDSVSILNDSQTLNSSKPEDSSAQEQFKQPVVQNEPKLELVVPVYDSAIAQV
                     PQPGDPYFDPPSQDRLMVVVEDEPAQEVLEFDVSREIPDESVARSSEVRRGALSGDLN
                     KGNAVAEVQGEPYEPLVVRMSDHIGGGVSVSEDEPILLVPSSFKDPLVEEVLAAENTV
                     SAKEAPVLDVLPPVFEKPEEIIVWNGGRVPVNTPIPVVDFEPASGTEQVDVVEEGQWR
                     ALEGQNIRQTLEQWSQAADVALIWDNANAFAVLEPFEIRGDYEQAVQMLLDQYMRSEV
                     RPVATLHLDQESQQKTLVVRIVEGG"
     gene            complement(3069..4185)
                     /gene="prfB"
                     /locus_tag="PK513_04595"
     CDS             complement(join(3069..4115,4117..4185))
                     /gene="prfB"
                     /locus_tag="PK513_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114581419.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOO81760.1"
                     /translation="MKAETESIVRDIEMALALLRRHLDWDVSVLRLDELNALSENPDI
                     WNDADKAQKVMRERNRLDDQISNVRALEQSLSDNVELIALGEEEGDEAVVAEAEQALE
                     VMKATVERRQLQSLLSGEADSNDAYLQVNAGAGGTEAQDWANMILRMYTRWAEGRGYK
                     VCVLEMSDGEEAGIKSATLKIEGDQAYGWLKTETGVHRLVRISPFDSNARRHTSFASV
                     AVSPVIDDDIDIEVEENDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVAAVQSER
                     SQHQNRANAMALLKAKLYEAELHKREEQAAAEHGEKTDIGWGHQIRSYVLHPYQMIKD
                     LRTGVETSQSAAVLDGGLDRFLEAALAQKLHRNEENS"
     gene            complement(4226..4819)
                     /locus_tag="PK513_04600"
     CDS             complement(4226..4819)
                     /locus_tag="PK513_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOO81761.1"
                     /translation="MSQYLRGQKIAVLVANGFSEKDLTLTQKEILNFGVQIRIVSMDA
                     GLVSSWNDDDGQESWGLNFASDQVLSRALAVDFDVLLVPGGQRSLDKLKMTAHTRRFI
                     RGFVEAGKTVIFFHDALELLAFAECGGGVRVSGPEGVREMLEVQGAVWVDDALVQSGA
                     VMSGRCDEDTRGDFTAAVAQFLMEQASAGAMSQAVAA"
     gene            5116..6036
                     /locus_tag="PK513_04605"
     CDS             5116..6036
                     /locus_tag="PK513_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOO81762.1"
                     /translation="MNTLRNMILTAGLLALGGTTSACTAFTSFSEVEALNDAQAVGSP
                     FTQALAEEYRRFANSELKDMFDYPDALHFARKGLAAASGENVLPEPVVDWNLSEEHIR
                     ELSAARGRLIVAYDLGGREVAPEIAAKAQAKYDCWIEGQEEAWQDSDGMACKDEFLKH
                     ISRLEGMIQPPPAAPAPEITADTGAFDIDPNAPMAVENAMYLVFFNWDSSDLGSGALN
                     VLDAVANEISKNPPATINVVGHTDTSGSQKYNQRLAFKRANNVKDALVQRGVDPALIL
                     VDAKGETELLVETPDNIREPANRRVNISFN"
     gene            6213..7655
                     /locus_tag="PK513_04610"
     CDS             6213..7655
                     /locus_tag="PK513_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOO81763.1"
                     /translation="MDFRPVLYIQGILLSILSIGMIIPMLADMYWGNPDWKVFFLCIV
                     ITAFFGGSLILSNAGLEFTMNLRQAFMMTFMSWVGLSLFASLPLTLAGLNMSFTDAFF
                     EAVSGITTTGSTVMTGLDTAPPGILLWRSILQWLGGIGIVLMAMSVLPMLSVGGMQIF
                     RTELSENEKALPRTAQLASSIGAIYVGLTMLCLLTYLLAGMGKFDALNHALTTLATGG
                     FSTYDASIGHFHSDIIELLCSFFMIIAGTPFILYLKAVRGNLRPLFVNSQVRTFLLIL
                     SLSIAATTTYLVITHDMSVYRAFIGATFNMTSLMTGTGFASEHYDQWGGFVIALTFFL
                     MAMGACAGSTSCGIKIFRFEIFLSVIRVQIQKLLHPHGAFVAHYNGKPIPEDVPLSVM
                     SFLFLYAICFIALALALSALGLDFITSLSGAMTSISNVGPGLGEVIGPSGNFSPLPDS
                     AKWVLSVGMLLGRLEILAALILLHPSFWRH"
     gene            complement(7662..8630)
                     /locus_tag="PK513_04615"
     CDS             complement(7662..8630)
                     /locus_tag="PK513_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOO81764.1"
                     /translation="MDDLISEGCLDAPGAFDEADDDVIQSFQLDVSSLRGRAVRLGSA
                     LNDILEPHDYPNPVAHLVAETVTVTLLLSSMLKFDGIFTLQIRGDGPVSLLVADMTSE
                     GQIRGCAHFDSERLEQARAQLLALKAEETSRNHLAQYLGKGYVAFTVDQGEAAERYQG
                     VVELRGASLVDCVQHYFNQSEQIISGIKMAVGQRGGVWRAGGVMLQKMPDGDGYSEIK
                     GNMDEDDWRRAMILLESCTEDEFLDPNLHSNALLTRLFHEEDVRVFQPVNVFKGCRCS
                     EEKVCTMLTMMSVEDLDYMAEDGRISMRCEFCSQEYSYAREKFPAS"
     gene            complement(8662..9465)
                     /locus_tag="PK513_04620"
     CDS             complement(8662..9465)
                     /locus_tag="PK513_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81765.1"
                     /translation="MKMPESLTPLHINTVNVIGLLTLIKREVDRFMNVYMQTLVAPVI
                     TTLLFYTIFALAFGGVARRVGDVPFLEFLAPGLIMMTMVQNAFANTSSSIVISKVQGS
                     IVDILLPPLSSFEMYVGFTVGAVARGLIVGLVSGTVMSFFVPISVSSWWLIIAYAVLG
                     TMMLGSLGLAAGIWSEKFDHIAAVTNFIVTPLTFLSGTFYAVEALPPVWQGLAHYNPF
                     FYMIDGFRAGFIGQADGNTLVGISLLIVINFSLAMLTLWMLRTGYKIKS"
     gene            9490..10086
                     /locus_tag="PK513_04625"
     CDS             9490..10086
                     /locus_tag="PK513_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOO81766.1"
                     /translation="MSQNNISNTNSKPQNNKRKRPKKITETYLHNAGLYYLERYASSA
                     ANFHDVMLRKVKRSCITHEDQDYTQCTALVDQLVKKFIRTGLLDDAVYTRAMVTSLRR
                     RGKSTRAIHSALRAKGLDSSQIEQTLVIIDQESHENPAEAERHAALTFARKKRLGPWR
                     SDKEINFQKELSCMARAGFSYDTSRSVLEISEDNIPFT"
     gene            complement(10094..12685)
                     /locus_tag="PK513_04630"
     CDS             complement(10094..12685)
                     /locus_tag="PK513_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOO81767.1"
                     /translation="MQGQPKGQQNSSINGFEHQRKGSDSAVLYVFLVVGLVLVFSTFT
                     VLQSVVRGAVVEEQVHVSQDFVEEIVSDFRGLSEPLKGLAAMMALSFEMKEDRLSRYI
                     NQEHSAFEAYSQIVWFYETPDKEWTFSYLRSNTLQNEINGGPQLKPDAEMLKVLLKLE
                     VSGDLRLVPNVGGFEQYVRSQNPKITYRPFALMKSVSAGGVRKGFLLAVVDVASLYDE
                     GWATGHQQVLSMRIQDVESNNVLFEFVRNPETTSATGGRQVYEFSFAGRHFEVASDFQ
                     VSQRVRLLGGFPYMVAFLAVFLVVAGAFFVHYGQKQAYNLKKMNDTLARKNFELKAEV
                     EERERLADAVEAAERSSRAVVDAIGDIIFEADRDGRIVFLNRAWEKVTGFDVGQSTGQ
                     NLEQLVYPQDREEVMRGLRAVLYEGKDEARSFTQIRVADGTFKAVELVMKAVARNTNG
                     GHRHDFVVGTFTNIEERRKVERALSDAEKKYRSIVENAVGGIFQMTSDGLYLSANPAM
                     AKILGYDSPEQLLRGVKNANEQVYVDTAKRQSVYDRLTSLPEALSHEVKMYKRDGEII
                     WVHENIRGVVDDSGQLLFIEGSVEDITRRKNSDLAIQEAKVHSDLANRAKSEFLANMS
                     HELRTPLNSIIGFSEMIKNEVCGTIQHRPYWEYACDIHESGHKLLQVINEILDISKIE
                     AGDRQLNESVVNIEAVFDSVAELLETKIENAHLHITRALGGVPNIIVEELALKQILLN
                     LLSNAVKFTPYDGRITVSAQLSREGDMHISITDTGVGLDEYEIKKALSPFGQVDNDLS
                     RSGSGTGLGLTLVGALVGLHGGSFDLFSKKGIGTTATVILPAERVVRDKREAASVSVK
                     EASEATE"
     gene            12916..13365
                     /locus_tag="PK513_04635"
     CDS             12916..13365
                     /locus_tag="PK513_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOO81768.1"
                     /translation="MSRQNPFAEFFTANDFSKFFENAQSMPFDLQGFMETQRKNMQAL
                     SDAQQIAIENLQTIAQRQSAIISEMVEDNAQITKELMSEGTPEEKISQNADLFKTLYE
                     RSVKNMNDIAEMMNKSNQEASSIINKRVKATMNEVKAAMDKSQKKAA"
     gene            complement(13455..14789)
                     /locus_tag="PK513_04640"
     CDS             complement(13455..14789)
                     /locus_tag="PK513_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO81769.1"
                     /translation="MSEGLPIKKFLPRTLFGRSLMILVTPVLLIQIITSFVFFDRHWS
                     KMTTRLAFAVSGELSVITATIKNGDTIRDIENIIAYSGRYLDLDVRFIPDGRLPDKQE
                     SSVVQVWESVVAQKLSAELATQISEPFVIHANFADKMIEVFIQLEDGLVRVSLPQRRL
                     FSSSGYVFLLWMIGASLLLLVVAVVFMRNQVRPIRKLAVAADRFGRGRAAPFFKPEGA
                     KEVRQAGHAFLEMRRRIERQVSQRTEMLAGVSHDLRTPLTRMKLQVEMMEEGLDKADM
                     QQDIADMERMIHGYLDFVRGDGDEEFEAVSLAEFFERLVAKMKHQGVKTDLMVAGGLS
                     LVVRPLAFERAISNLLTNAGKYADHIWVSARKDGEKLQIVIEDNGPGVAEDQYEAVFK
                     PFMRVDSSRNAETGGVGLGLPIAMDIIHAHGGKIWLEASEYGGLKVMVRMPL"
     gene            complement(14793..>15045)
                     /locus_tag="PK513_04645"
     CDS             complement(14793..>15045)
                     /locus_tag="PK513_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO81770.1"
                     /translation="ARAGEEVVRLTSAEAQLLKALGARGGEVLSREELARACGVDAGE
                     RTIDVQVTRLRRKIENDTKKPRYLQTVRGKGYVLRSEEL"
BASE COUNT         4324 a         4567 c         2678 g         3476 t
ORIGIN      
        1 actttctttt caccttgagc agccaacgct ttcaaagcat cacgctctgc ctccagatct
       61 gcaattctcg ccacatgagc gttagcgtcc gcctccgccg catccgatcc cgcagacaaa
      121 ctttgctgcg ccacatcaag ctgcgcacgc aaatcctcat tctcagcctc aaggttttca
      181 atgcgcgtta ccatcacatc actttgacta cgctgcactt cttgaaaagc cagcttttct
      241 tttaaagcag cattctcatc acgcagcgtc gcaagcgcag ccgttccctc actaatttca
      301 gtcagcgcct tcgtcttaga ctcactttca gccttcaacg catcaacttc attttctagc
      361 gctgcaatcc taactgcttg ttcattacgc accttttcaa ccgttttttc ctgctcttca
      421 tgaaattgcg tttcaagatc cccttgcgcc ttcgcagccg tttcctgcaa caaacgattt
      481 tcattttgca cctgagcaat ctgatgtcga agctgctcca aaagctcagc gtccaccacc
      541 ttacttcccg cagatgtttt caaagcagca ttctcattat tcagcgcagc aatctgttgt
      601 ttaaactcac tacgcacagc ctcgcgcgcc gcaagaattt tagtgctaag ctgtgaattc
      661 tcattctcca gttcctcaat ttgcgaacgc aacccatcaa ccaactcttt cccttcaccc
      721 tgcgcatcaa ggcttgcttg caaattccca ttacgcgttt gcaaggactg gacttcagct
      781 tccagagcag taatgcgctc aagatcatga gaagattgaa cgacctcgcc tgtctgtaac
      841 aaggcctgca accgagcatt ttcgttttcc aacgtaacaa tctttgcgtc caatgctcgc
      901 acatcacttt ccactaaact ctcaccagct tgtgccgttt gtgctgcaga aagctgccgc
      961 ttcaacaatg cattcgcatc ctctaactca cggattttcc ctgataactg cacaacagaa
     1021 gaagacgtct cctctacgcc ttcatttcga gtgctcattt gcaccttcaa cgccgcattc
     1081 tgctcctcta aagacgtaat ctgccgccgc agcgcattaa tctcctgacg gaaagatttc
     1141 ccggcattca caacagccgc atccatatca ggcataagcg atccatcttc accagccgca
     1201 ggcatcacca tgttaacaac acacgcatca agcttgaact gtccctcatc tatatcggcc
     1261 acattaaaat gataagactg cccatccgtt tccacgcgca acaaccctgt ccgagcaagg
     1321 gcatcaaaaa atgaagaatc ccgccccaaa cgcacaacaa acgccttatc tgaaaccggc
     1381 ttaatttcat aagcacgctg ctgaccagct cctggatcca aaataacctt catcgccgct
     1441 tgctgattca aaataggccg ttgaaaatcc agcgcaaacg aggtctccga aaactcatta
     1501 cgtgccaatg tcaaaaccgt atcttgatta tatttccgcg ctaacgcaca atacgcaccg
     1561 ccagcatcac caatcttgga aacagcccac cctgtagacg gtgccattat ggagcccttt
     1621 tgcgccaaag cgtcgccaac aagcgccgaa ctggccaaaa acgcacctaa aacagccact
     1681 gaagaaacca aagatcgaat acgcatcgct cttttccctt aaatgcaatg aaaatcaaaa
     1741 taaagacagg acattacaaa actcacttcc caaaacacga gcaaaagaat cataaaccga
     1801 aacccccact ccagcttacg gtcataagga tatgagtcaa aacttattca cattctgcaa
     1861 gctaggcctg tgggtaaaat ctcgctaccc accctcaaca atccgcacaa ctaacgtctt
     1921 ttgctggctt tcctgatcca aatgcagtgt tgcaaccgga cgaacctcac tacgcatata
     1981 ttgatccaaa agcatctgaa ctgcctgctc ataatcaccg cgaatctcaa atggttccaa
     2041 aaccgcaaaa gcgttagcat tatcccaaat caatgccaca tcggcagcct gcgaccattg
     2101 ctcaagcgtc tgacggatat tctgcccttc caacgcacgc cattgcccct cctcaactac
     2161 atcaacctgt tccgtaccgg acgctggctc gaaatccacc accggaatag gagtattaac
     2221 cggcacacga cccccgttcc acacaataat ctcctcaggt ttttcaaaga ccggtggcaa
     2281 aacgtctaaa accggcgctt cttttgcaga aaccgtattt tcagccgcta gcacctcttc
     2341 aaccagagga tctttaaacg aagaaggcac aagcaaaata ggctcatctt cagaaactga
     2401 tactccccca ccgatatgat cagacatgcg aaccaccaaa ggctcataag gctcaccctg
     2461 aacctctgca acagcattcc ctttatttaa atcgccggaa agagcccccc tccgaacctc
     2521 agatgacctg gcaacgctct catcaggaat ctcccgtgac acatcaaact ccaatacctc
     2581 ttgtgcaggc tcgtcctcca ctaccaccat caaacggtcc tgacttggcg ggtcaaaata
     2641 cgggtcaccc ggttgaggga cctgagctat agcagaatca tacaccggca ccacaagctc
     2701 gagtttaggc tcattctgga ctacaggttg cttaaattgc tcctgtgcgc tggaatcctc
     2761 cggttttgaa gagttcagcg tctgagaatc attcaaaatg gaaaccgaat cacgcagcgg
     2821 tggcaaaaac tgaaatccag cctgaatcac agactgcgca acctgctctg acgaatatgg
     2881 atcaaccgtc aacgggttcg ccagcgcccc gccactcatg accatcccca ttaaaagaac
     2941 tgttccacaa acctgagtac gcataattga aaatccctat aaaattgtaa atttacgaat
     3001 cagcctaatg taacaccatc gatataacaa gcccaccaac tcgaccaaac agcttttatc
     3061 gcttagagct atgagttttc ttcattccga tgaagctttt gcgccaacgc cgcttccaag
     3121 aaccgatcaa gccccccatc caacacggcc gcagattgtg aagtttcaac ccccgtgcgc
     3181 aaatccttaa tcatttgata cggatgcaaa acataagagc gaatttgatg cccccaccca
     3241 atatcggttt tttctccatg ttctgccgcg gcctgttctt cgcgcttatg caactccgct
     3301 tcataaagtt tggccttaag cagtgccatt gcattggcac ggttctggtg ttgtgagcgc
     3361 tcactctgta ccgctgcgac aatccctgtc ggtatatgcg taatacgaac cgcagaatcc
     3421 gtagtgttaa catgttggcc acctgctcca ctgctacgat acgtatcgat acgtaaatca
     3481 ttttcctcaa cctcaatatc gatgtcatca tcaatcaccg gcgagaccgc aaccgaggca
     3541 aaacttgtat gccgccgcgc attcgaatca aacggtgaaa tccgcaccaa acgatgcacc
     3601 cccgtttctg tcttcagcca gccgtaagcc tgatcgcctt caattttcaa agtagccgac
     3661 ttaattccgg cctcttcccc atcactcatt tccaaaacac aaaccttata tccgcgccct
     3721 tctgcccaac gcgtatacat ccgcagaatc atattagccc aatcctgcgc ctctgtcccg
     3781 ccggccccgg cattgacctg caaatacgcg tcattgctgt cagcctcacc cgaaagcaag
     3841 ctctgtaact ggcggcgttc aaccgtcgct ttcatcacct ccaaagcctg ctcagcctca
     3901 gccacaacag cctcatcacc ctcttcttcc ccaagagcga tcaattcaac attatcactt
     3961 aaagattgct ccaacgcccg cacattggaa atctgatcat ccagccgatt acgctcgcgc
     4021 ataactttct gagccttgtc agcatcattc caaatatccg ggttttcact taaagcatta
     4081 agttcatcga gacgcaaaac agaaacatcc cagtcaaaga tgcctcctca gcaaagccag
     4141 agccatttca atgtctctta caatgctttc agtttcagcc ttcaagttaa accccttaaa
     4201 atcgaacgct ccaccaaaga gctacctaag ctgccacagc ctgcgacatc gcaccggcag
     4261 aagcctgttc cattaaaaac tgcgccaccg ctgctgtaaa gtctccgcgt gtatcttcat
     4321 cacagcgccc gctcatcaca gcgccacttt gcaccaatgc atcatcaacc caaaccgcac
     4381 cctgaacctc cagcatctca cgcaccccct caggtccact cacacgcacg ccgccgccgc
     4441 attcagcaaa agccagtaat tccaaagcat catggaaaaa tataaccgtc ttacccgcct
     4501 caacaaagcc gcgaataaat cgccgcgtat gcgctgtcat tttaagcttg tcaagactgc
     4561 gctgaccacc aggcaccagc aacacatcaa aatcaaccgc caaggcccga ctcaaaacct
     4621 gatcggaagc aaaattcagc ccccagcttt cctgcccgtc atcatcattc cagctactca
     4681 ccaatcctgc atccatactc acaatgcgaa tctgcacacc aaaatttaaa atctcctttt
     4741 gcgtcaaggt caggtccttc tcagaaaacc cattagcaac gagcacagca attttttgac
     4801 cacgcagata ttggctcatt caaaaactcc ttaatttata agcatttaac gttttgattc
     4861 ttttaagaaa atcgaaaaac cgggcagaca tctttacaaa agatgtatcg acacaccgga
     4921 attttgccac tttatttatc gtctgacacc aaagagtcaa gtttttattc cccacgccaa
     4981 aaaacccacc agaaaaaatc ctctggaaaa gcaatcccaa agccctaaac acgctttaca
     5041 atacggaata agggctttaa aagcataagt attattaaaa agcatgcttt caatactggt
     5101 ttaaggagca cataaatgaa cacgctgcgc aatatgattt taaccgctgg cctgctggca
     5161 ctggggggaa cgacgtccgc ctgcactgcc ttcacaagct tcagcgaagt agaggcctta
     5221 aatgacgccc aagccgtggg cagcccgttc acccaggccc ttgccgaaga atatcgacga
     5281 ttcgccaaca gcgaacttaa agacatgttc gactaccctg acgcactaca ctttgcgcgc
     5341 aaaggtctgg cggcagcctc cggcgaaaat gttttgcccg aaccagttgt tgactggaac
     5401 ctgagcgaag aacatatccg cgaactcagc gctgcgcgcg ggcgtttgat cgtcgcatat
     5461 gatcttggcg gacgcgaagt tgctcccgaa atcgccgcca aagcacaggc aaaatacgac
     5521 tgctggattg aaggacagga agaagcatgg caagattcag atggaatggc ttgcaaggat
     5581 gaattcctga aacatatcag tcgactcgaa ggcatgattc agccgccacc cgccgctcca
     5641 gcccctgaga taaccgccga cacaggtgcg tttgacattg accctaacgc ccctatggca
     5701 gtcgaaaacg ctatgtatct ggtcttcttt aactgggatt catcggatct cggctcagga
     5761 gcacttaacg ttctcgacgc cgtcgctaat gaaatctcta aaaacccgcc tgcaacaatc
     5821 aatgttgttg gacacactga tacatcaggc tctcagaaat ataaccaacg tctggcattc
     5881 aaacgcgcca acaatgttaa ggatgccttg gtacaacgtg gcgttgaccc ggccctgatt
     5941 ttggtcgacg ccaaaggcga aacagagcta ttggttgaaa caccggacaa tatccgcgaa
     6001 ccggcaaacc gccgtgtgaa tatctctttc aactaaaaga gtaatcgcat aaaatcagag
     6061 ccgaagcata gcctgcctgt gcttcggctt tttatagcgc tcacaatagt cgcttgaatt
     6121 tctcttctga caagacgcaa gcgacgacaa atagaaaacc tatttcagaa gctaagcaac
     6181 gctgtcagaa gataaagaca aggctactat ttatggattt tagacctgtt ctatacattc
     6241 aaggtatttt actttccatc ctgtctatcg gcatgatcat tccaatgctc gcagatatgt
     6301 attggggcaa ccctgattgg aaagtctttt tcctatgtat cgtcatcaca gcttttttcg
     6361 gcggatcttt aattctgagc aatgccggtc tcgaattcac catgaatcta cgccaagcct
     6421 tcatgatgac cttcatgagt tgggttggcc tgtccctctt cgcttccctc cccctaacgc
     6481 ttgcaggact aaacatgagc ttcacggatg cgttttttga agccgtctcc ggcatcacca
     6541 ccaccggctc cacagtcatg actggcctgg acaccgcccc ccccggcatt ctgctctggc
     6601 gttcaattct acaatggcta ggcggcattg ggattgtcct gatggccatg tccgtcctgc
     6661 caatgctcag cgttggtggc atgcaaattt ttcgaaccga actctcagaa aatgaaaaag
     6721 ccctaccccg tacagctcaa ctcgccagct ccatcggcgc aatttatgtc gggctaacca
     6781 tgctttgtct actcacatat ttattggcag gtatgggaaa atttgacgcc ctcaaccatg
     6841 ctttaaccac attagcaacc ggcggattct ctacctacga tgcctctata gggcacttcc
     6901 acagcgacat aatagagtta ctctgctcct tttttatgat cattgcaggg acccccttta
     6961 tcctgtattt aaaagccgta cgaggcaacc tcaggcctct atttgtaaat tcacaagtac
     7021 gcacattttt gctaattctc agcctttcaa tagcggcaac cacaacctac ctagtcataa
     7081 cccacgacat gagtgtatac cgggcattta taggcgctac atttaacatg acttcattga
     7141 tgaccggcac aggctttgcc agcgaacatt acgaccaatg gggcggcttc gttattgccc
     7201 tcaccttctt cctcatggcc atgggcgcct gcgcaggatc aacctcctgc ggcatcaaaa
     7261 tcttccgttt tgagattttc ctatctgtca tccgtgtaca aatacaaaag ctcctgcatc
     7321 cgcacggcgc ctttgttgcc cactacaatg gcaaacccat ccccgaagat gttccgctct
     7381 cagtgatgag cttcctcttc ctctacgcca tatgcttcat cgctctcgca ttggcgctct
     7441 ctgctctagg actggatttc ataacatcgc tttccggcgc catgacctcc atatcgaatg
     7501 taggtcctgg gctgggtgaa gtcatcggac catcaggaaa tttttcaccc ctccccgaca
     7561 gtgccaaatg ggtcttaagt gtcggcatgc tgctaggacg cctcgaaatc ctggccgcac
     7621 ttatcctgtt gcacccgtca ttctggcggc attaatgctt tttagctggc aggaaacttt
     7681 tcacgcgcat agctatattc ctgagaacaa aattcacagc gcatggaaat ccgaccatct
     7741 tctgccatgt aatcaagatc ttcaaccgac atcatcgtta acatggtaca gaccttctct
     7801 tcactgcacc gacaaccttt aaagacattc acaggctgaa acacccgtac atcttcttca
     7861 tgaaataacc gcgtcagcag cgcattagaa tgcaaattcg gatcaagaaa ctcatcttcc
     7921 gtacagcttt ccagtaaaat catcgcccga cgccagtcat cctcatccat attacccttg
     7981 atttccgaat aaccatcacc atctggcatt ttttgaagca tcacaccacc agcccgccaa
     8041 acgccgccgc gctgccccac ggccatctta atcccgctga taatctgctc ggattgatta
     8101 aaataatgct gcacacaatc caccagcgaa gccccacgca actcgacaac cccctgataa
     8161 cgctccgccg cctcaccctg gtcaactgta aaagcgacat agcccttccc cagatattgc
     8221 gccaaatgat tacgcgaagt ctcttcggcc tttaaggcca agagctgcgc ccgcgcctgc
     8281 tctaaacgct cagaatcaaa atgcgcacac cctcgaattt gcccttccga tgtcatatca
     8341 gccactagaa gactcaccgg accatctccc ctgatttgca aggtaaatat cccatcaaat
     8401 ttcagcatcg aagaaagcaa aagcgtcacc gttaccgttt cggcaaccaa atgcgccacc
     8461 ggatttggat aatcatgcgg ctcaagaata tcattcaacg cgctgcccaa ccgcacagcg
     8521 cgcccccgca atgacgaaac atccaactga aaactctgta taacatcgtc gtctgcttca
     8581 tcaaaggcgc cgggcgcatc aaggcacccc tctgagatta aatcatccat cccttcatta
     8641 tagcacgaaa acacagggct tttacgactt gattttataa cccgtacgca acatccaaag
     8701 cgtcagcata gccaaagaaa aattaataac gatcaaaaga ctaatgccaa ccagcgtatt
     8761 tccatccgcc tgcccaataa acccggcgcg aaatccgtca atcatgtaaa aaaacggatt
     8821 ataatgcgcc aatccttgcc aaaccggcgg caaagcctcc accgcataaa atgtccctga
     8881 taaaaaggtc aaaggcgtta caataaaatt tgtcaccgct gcaatatgat caaatttttc
     8941 cgaccaaatt ccggccgcca gccccaaaga accaagcatc atcgtaccca aaacagcata
     9001 ggcaataatc aaccaccacg aactcacaga aatcggtaca aaaaaactca tcaccgtccc
     9061 actcaccagc ccgacaatta acccgcgagc aacagcaccc acggtaaaac caacatacat
     9121 ttcaaagctc gacaatggtg gcaacaatat atcaacaatg ctgccctgaa ccttggaaat
     9181 cacgatagag gacgacgtat tggcaaacgc gttttgtacc attgtcatca taatcaaccc
     9241 cggagctaaa aactccagaa acggcacatc accaacccga cgtgccacac caccaaacgc
     9301 cagtgcaaaa atcgtataaa ataacaacgt tgtaataacc ggcgcaacca atgtttgcat
     9361 atacacattc ataaatcggt caacttcacg cttaatcaac gtcaaaagac cgataacatt
     9421 gacagtgttg atatgaaggg gtgttaagga ctctggcatt ttcatcatat tttcgttaaa
     9481 attcatacca tgtcacaaaa taatatttcc aacaccaata gcaagccaca aaacaacaag
     9541 cgcaaacgcc caaaaaaaat taccgaaacc tatctccaca atgccggact atattacctt
     9601 gaacgctacg cctccagcgc cgcgaacttc cacgatgtca tgctccgcaa agtcaaacgc
     9661 tcctgcataa cccatgaaga tcaagactac acccagtgca ccgcattggt agaccaactg
     9721 gttaaaaaat ttatccgcac aggcctgctc gacgatgccg tctatacccg cgccatggtc
     9781 acatccttgc gtcgccgcgg aaaatccacc cgcgccatcc acagcgcctt acgcgccaaa
     9841 gggctcgatt ccagtcaaat cgaacaaaca ctggtcataa tagatcagga atcgcacgag
     9901 aacccagccg aagccgagcg ccacgccgct ctcacatttg cacgaaaaaa gcgcctggga
     9961 ccctggcgca gcgacaaaga aataaatttt caaaaagagc tctcatgcat ggccagagcc
    10021 ggattttctt atgatacgtc gcgaagcgtt ttagaaataa gcgaagacaa tatccccttc
    10081 acataaatac acattattcc gtcgcctcag acgcctcctt aaccgaaaca ctagcggcct
    10141 ccctcttatc ccgaacaaca cgctcggcag gtaaaataac cgttgccgtt gtccctatac
    10201 cctttttcga aaacaggtca aacgaaccac catgaagccc cacaagcgca ccaaccaatg
    10261 tcagtccaag ccccgttccc gaaccgctgc ggctcaaatc attatcaacc tgcccaaaag
    10321 gagaaagcgc ctttttaatc tcatattcgt caagtccaac ccccgtatcc gtaatcgaaa
    10381 tatgcatatc accttcacga ctaagctgcg cactcaccgt aatgcgccca tcgtagggcg
    10441 taaacttgac cgcgttagaa agcaagttca acaaaatctg cttcaaagca agctcttcca
    10501 cgataatatt gggcacacct cccaacgccc gagtgatatg caaatgagca ttttcaatct
    10561 tagtctcaag caactcggca accgaatcaa aaaccgcttc aatattaacg acactttcat
    10621 tcaactgccg gtccccggcc tcaatttttg aaatatccag aatttcgtta ataacctgca
    10681 acaacttatg cccgctctca tgaatatcac aggcatattc ccaataagga cgatgctgaa
    10741 tcgtcccaca cacctcattc ttaatcatct cagaaaaccc gataattgaa ttaagcggcg
    10801 tacgcaattc gtggctcata ttcgccaaaa actcggactt cgcgcggttc gcaaggtcag
    10861 aatgcacttt cgcttcctga atagccaaat cagagttttt ccgacgggtt atatcctcaa
    10921 cacttccctc aataaataaa agctgcccgc tgtcatcaac aacgccacga atattttcat
    10981 gaacccaaat aatctcccca tcgcgcttgt acattttaac ctcatgcgaa agcgcctccg
    11041 gcaaactcgt aagcctgtca taaacacttt gacgcttcgc cgtatcaaca tagacttgct
    11101 cattggcatt tttgacccca cgaagtaact gctccggact atcatagcct aaaatcttcg
    11161 ccatcgccgg attggcactt agataaagcc cgtcagacgt catctgaaaa atcccgccaa
    11221 cagcattttc aacgatagaa cgatacttct tctccgcatc gctcaatgcc cgttcaacct
    11281 tacggcgctc ctcaatattg gtaaaagtcc caacaacaaa atcgtgacgg tgtcccccgt
    11341 ttgtattacg agcaacagcc ttcataacca gttccacagc cttaaaggta ccatcagcca
    11401 cgcggatttg cgtaaaactg cgtgcctcat ccttgccctc atacaaaaca gcccgcaaac
    11461 cacgcattac ctcttcacga tcctgaggat aaacaagctg ctcaagattc tgccccgttg
    11521 attgccccac atcaaaaccg gtcactttct cccaggcccg gttcaaaaaa acaattcgcc
    11581 cgtcacgatc agcttcaaaa ataatatcac caatcgcatc aacaaccgcg cggctgctcc
    11641 gctccgccgc ctctacagca tccgcaagac gttcacgctc ttcaacctca gctttcagct
    11701 caaaattctt ccgcgccaag gtatcattca tttttttaag attataagcc tgcttctgcc
    11761 cataatgaac gaaaaatgcc ccggcaacca ccaggaaaac agccaaaaaa gcaaccatat
    11821 agggaaagcc gcccaacaac ctcacacgct gcgaaacctg gaaatcactc gcaacctcaa
    11881 aatgacggcc cgcaaaagaa aattcataaa cctgacgtcc ccccgttgca gatgttgtct
    11941 cgggattacg gacaaactca aaaagcacat tattactttc aacatcttgt atgcgcatag
    12001 atagaacctg ctgatgccct gtagcccaac cctcatcata aagagaggct acatcaacaa
    12061 ctgccagtaa aaatccttta cgcacccctc ccgcgctcac agatttcatt aaagcaaacg
    12121 gacggtaagt gattttagga ttctgactgc ggacatattg ctcaaagcct ccaacgttag
    12181 gcaccaaacg caaatcacca gagacctcaa gcttcaaaag aactttaagc atctcagcat
    12241 ccggtttaag ctggggtcca ccattaattt cattctgcaa cgtattgctt cgcaaatacg
    12301 aaaacgtcca ctctttatca ggggtttcat aaaaccagac aatctggcta taggcctcaa
    12361 acgctgaatg ttcttgatta atatagcggc ttaatctgtc ctctttcatc tcaaaagaca
    12421 aagccatcat ggccgccaac ccctttaatg gttctgacaa accacgaaaa tcactaacaa
    12481 tctcttcaac aaaatcctgt gaaacgtgga cttgctcttc cactacagcc ccgcgaacaa
    12541 cactttgcaa caccgtaaat gtgctaaaaa ctagcactaa tcccacaact aaaaatacat
    12601 aaaggacagc agaatcggag ccttttcgct gatgttcaaa gccgttaata gaactatttt
    12661 gctgcccttt aggttgcccc tgcatgatgt aaactcattt gtaattcgtg cattatcccc
    12721 ttgcacaccc ccataaagat gcctgaaaac ccttaaaaaa atgctaaaat acgaaacgtt
    12781 ctaaaaaacc ctgaaaaaca aagctataag acaggcctgt tctctacatt ttttcatttg
    12841 tgcactgcta cgctttaatg taacaatggc aacagcaaac ctgtggcatt caccattttg
    12901 aaaaggaata tcaccatgtc gcgacaaaat ccttttgctg agtttttcac cgccaacgat
    12961 ttttcaaaat tttttgaaaa tgctcaatcg atgccttttg acctgcaagg tttcatggaa
    13021 acccagcgca aaaacatgca agccctgtcc gatgcacagc aaatcgccat tgaaaacctt
    13081 cagacaattg ctcaacgcca aagtgccatc atctcagaaa tggttgaaga caatgcacaa
    13141 atcaccaaag aattgatgag cgaaggcaca ccggaagaaa aaatctccca aaacgccgat
    13201 ctgttcaaaa cactctatga gcgcagcgtc aaaaatatga acgatattgc tgaaatgatg
    13261 aacaaatcta atcaggaagc cagcagtatc atcaacaaac gcgtcaaggc cacaatgaac
    13321 gaagtcaaag ccgcaatgga caaatcgcag aaaaaagcgg cctaactaac gctttatata
    13381 tttgctttcc ctacgaaaac agggagcttc ttctctcatg aacataaact attcatagat
    13441 tgcagttgcc ttttctaaag cggcatccgc accataacct ttaaaccgcc atactcactg
    13501 gcctcaagcc aaatcttacc gccatgcgca tgtataatat ccatcgcaat cggcaacccc
    13561 aaaccaacgc cgccagtttc cgcgttgcgt gagctatcaa cccgcataaa gggcttaaac
    13621 accgcctcat actgatcctc cgccaccccc ggcccgttat cctcaatcac aatctgtaac
    13681 ttttcgccgt ctttcctggc gctcacccag atatggtccg catacttccc tgcattcgtt
    13741 aacaaattgc tgatcgcgcg ctcaaacgca agtggccgta ccaccaaact caatcctcct
    13801 gcaaccatca aatccgtttt tactccctga tgtttcatct tcgcgaccaa cctctcaaaa
    13861 aattctgcca acgaaacggc ctcaaattcc tcatcaccat ccccgcgcac aaaatcgaga
    13921 tacccatgaa tcatccgctc catatcggca atatcttgct gcatatcagc tttatccagc
    13981 ccttcctcca tcatctcaac ctgcagcttc atccgcgtca atggtgtgcg caaatcatga
    14041 gacacaccgg ccagcatctc tgtgcgctgc gaaacctgac gctcaatacg tcggcgcatc
    14101 tccaaaaacg catgaccagc ttggcgcacc tcttttgccc cttccggctt aaaaaacggc
    14161 gcagcgcgcc cccgtccaaa tctgtccgcc gccaccgcca acttgcgaat aggccgcacc
    14221 tgattacgca taaacaccac cgccacgacg agcagcaata aagacgcccc tatcatccac
    14281 aacaaaaaca cataaccaga cgaagaaaac aagcgccgct gcggcaagga aacacgcacc
    14341 agcccatcct caagctgaat aaacacctca atcatcttgt cggcaaagtt ggcatgaata
    14401 acaaacggct cagaaatttg cgtcgccagc tccgctgaaa gcttttgcgc cacaacgctc
    14461 tcccatactt gcacaactga agattcctgt ttatctggca accgcccatc gggaataaaa
    14521 cgaacatcca aatccagata ccgccccgaa tacgcaataa tattttcaat atccctaatt
    14581 gtatcaccat tcttaatcgt tgccgtaatc accgacaact cacccgaaac ggcaaaagcc
    14641 agccgtgttg tcattttgct ccaatgacgg tcaaaaaaca caaaactggt aataatctgg
    14701 atcaacaaca ccggcgtgac caaaatcatc aacgaacgtc caaacagggt tcgcggcaaa
    14761 aactttttaa taggcaaccc ttcgctcatt ccctaaagct cctcactcct aagaacatac
    14821 cctttaccgc gcactgtttg taaatagcgc ggtttcttgg tgtcgttctc aatttttcgc
    14881 ctgagccggg ttacctgcac atcaatcgtg cgctccccgg catccacgcc gcacgcgcgg
    14941 gccaactctt cgcgggacaa gacctctcct ccacgtgcac ccaacgcctt aagcaactgc
    15001 gcctccgcgc tcgtcaaccg caccacctcc tcgcctgcgc gtgcg
//
LOCUS       DAOSWR010000042        14988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2504_length_14988_cov_5.623518, whole genome shotgun sequence.
ACCESSION   DAOSWR010000042 DAOSWR010000000
VERSION     DAOSWR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14988
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2504_length_14988_cov_5.623518"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..234)
                     /locus_tag="PK513_04650"
     tRNA            complement(158..234)
                     /locus_tag="PK513_04650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ile,seq:cat)
     gene            complement(484..1749)
                     /locus_tag="PK513_04655"
     CDS             complement(484..1749)
                     /locus_tag="PK513_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81771.1"
                     /translation="MYEQVFQDIKGVGIATLNDFRSEASGLARQDIRKGMLKVVFSDG
                     RAVGVAAPYATGIALAGRNNLEEVAFTSFQKRFSVDWTGEKARASKVIAVTIDNIASK
                     GFVFLSKGMIASQWERLQVRLSPEIAYDLSKNIGIPVWPWVELEAKGCELITPSGART
                     FPRISISSFCHKAELMKEFNEYGNPVCLTYTEGKRDVEKAIMAPSYYLDDLREIKRAN
                     VKEIPAKFITQTPAAQWTIPDNEIWLMKDATNHNVDPQAIFLPAELPASVADFFSAQN
                     SFAPLKVDELDNDKFYDLSAGAKKPGLSDILLQIAFNIQNKEGGDSRFINILPRYKRN
                     SPHQLLICNDLHGAFNLLVKTPVEGGLLNVKNIVQADMRNALNSALCRLERATVAQIS
                     LSAGGNPILMVKNDQRAMGAVLHARDLIM"
     gene            complement(1906..3837)
                     /gene="rpoD"
                     /locus_tag="PK513_04660"
     CDS             complement(1906..3837)
                     /gene="rpoD"
                     /locus_tag="PK513_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOO81772.1"
                     /translation="MAVSEKQTDDNQDTEGKGSLANVVKKLVKIGKDKGFLTYDEINK
                     AIPAAEFSSEQVDDAMATFSDIGIQLVEKEDDVAEESGDDNQRGHEAGGNIADDDTGR
                     TDDPVRMYLREMGAVELLSREGEIAIAKRIEAGRELMIGGICESPLAIESIIAWYKAL
                     QDGDILLREVIDLDATYNEPPEDATAEPEEDESDEDAEDRDEDSDDENDDESSDSDGD
                     DEVNISLAAMEEELAPQVFEMFGQIQKTYKKMQKVQQVRLDALQNGKEPAEADNKKYN
                     QLKEEMVEHMNEVQLNNARIEQLLDRLYSMNRELVGVEGKLMRMAVDAKVKREDFLEN
                     YYGSELDPKWLARVSKLKGKGWDKFTSNNKEQIEKIREQIALISEEAMLPISEWRRII
                     GTVQKGEKEAARAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFE
                     YRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMMHEIGREP
                     TPEELAERLHMPLDKVRKVLKIAKEPVSLETPIGDEEDSSLGDFIEDKNAIAPIDSAI
                     HSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFNVTRERIRQIEAKA
                     LRKLKHPSRSRKLRSFLDT"
     gene            complement(3956..5800)
                     /gene="dnaG"
                     /locus_tag="PK513_04665"
     CDS             complement(3956..5800)
                     /gene="dnaG"
                     /locus_tag="PK513_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOO81773.1"
                     /translation="MSISPRFLDELRNRLTLSEMIGRRISVQRAGREFKACCPFHNEK
                     TPSFTINDDKQFYHCFGCGAHGDVIGFVMAHDNVSFIDAVETLCAEAGMQMPKPDPMA
                     VEKAEKAKGLHDLMDEATRWMQDQVIQPQNTDILEYLEGRGMDKVTRANFRIGYAPAD
                     GQALRKYLRAKGFSDAQMLEVGLLKDPNRGAEPYIFFRDRVMFPVCDRRGRVVAFGGR
                     ILPESMRPQTGKNFTPPKYINTADTPLFDKGRMLYAESMARQAAREGHTLIVTEGYMD
                     VIACNKYGFKGAVAPMGTALTEDQILVLWSMMVDEDKIPILCFDGDNAGRKAASRACE
                     RLLPLLQPGKSARFAFLPEGEDPDSLLRASGAGGFKAVLSASLSLFDFLWITHTAGRD
                     LDTPEARAGLTKALQKQVSVIGDANVQRHYRELVRARISEKFFRPYQNKGGLRSQGSA
                     PKPKSSIRLRPPLQKKNVLSQRILLASMINNPALYHKVEEEFGQFIINDPAMERLRQE
                     IIVFLGNDDALDTASLSTHLNSCGFEQEMHDILNESVYVHAAFARIGAFETMEQGEAV
                     KNWLDLYHALQGRETRGEVCAEWKTAFETSNEEQEDRLRDWVRIKNAE"
     gene            6169..6300
                     /locus_tag="PK513_04670"
     CDS             6169..6300
                     /locus_tag="PK513_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81774.1"
                     /translation="MPEIFSTGAKFKLVEYGSNQQGLVSIAYVKDKLVELLQRIAQK"
     gene            complement(6371..6829)
                     /locus_tag="PK513_04675"
     CDS             complement(6371..6829)
                     /locus_tag="PK513_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOO81775.1"
                     /translation="MEKREEFTAALKDALKAKDQTAMSTIRLILAALKDRDITARGQG
                     KADGIEESEILSMLQSMIKQRQEASKIYCDAGREELAEREEAEIEVIQKFMPVQLGEE
                     EVAKAVEKIIGDTGATGIKDMGKVMGALKSQYAGQIDMGKAGAIVKARLG"
     gene            6928..8100
                     /gene="carA"
                     /locus_tag="PK513_04680"
     CDS             6928..8100
                     /gene="carA"
                     /locus_tag="PK513_04680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOO81776.1"
                     /translation="MTQVPTLKQPSEASAVILFADGTSFFGRGFGAQTKAVGEICFNT
                     AMTGYQETLSDPSYAGQIITFTFPHIGNVGTNNEDLESLNPAARGLIVREDVTNPSNW
                     RAQSRLDEWLKSHNLPGISGIDTRALTRYIRDNGAPNSVLVHDLSGKFDLEALHKEAQ
                     DWPGLEGMDLAKEVSCTQSYSWKEKCWTLEEGYTEQDHPEYKVVAVDFGAKRNILRCL
                     ADTGFDVMVVPATSTAEEILAHKPDGVFLSNGPGDPAATGEYAVPMIRALLDQGMPIF
                     GICLGHQLLALALGAKTRKMHLGHRGANQPVKDLLTGKVEITSQNHGFEVIAESLPEG
                     TEQSHLSLFDGSNEGLRVKDKPAFSVQYHPEASPGPQDSYYLFERFKENIQKAKAA"
     gene            8232..9584
                     /locus_tag="PK513_04685"
     CDS             8232..9584
                     /locus_tag="PK513_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOO81777.1"
                     /translation="MSQITSQETVSVDDLSQGMTAPGKVNHFLEGYFRHTDIDRIPEK
                     LMFIIACADSREDMEVKFGLNYGEALFYRAVGAVVPDPDDEIASLPMKAALEHALKNP
                     HQVEIALLIPHTHCGAVKAHMTGEGPKALLTAMSGTTNFSGISGSIMTPEFKLVSDPD
                     EVMAVLRQENMNEGVKHAVLRSAERACMDVSRDNLLKYPQIKEGLKSGKFKLLTGLDD
                     IACDNISFQMDGHSEGKNITERFLHRYKNATEGLYDSENGGRMPELVLNGQHPEALFI
                     SGLSGDLGKVFSVRIGDAFVHRHTFGLIPPPRQDTSQPDPLAATIALALISGVETFIH
                     KFHADGTFRNYMDGDLLPYSSQWLEYCIPDLSQMKNSGASLDQVRLHCMLKSIEHLKL
                     YDGVQAAMNARKLSVLSLYEDPEKRELFVPDRDKNCFVKVLLPDENPAPQENRAFELA
                     "
     gene            9787..10434
                     /locus_tag="PK513_04690"
     CDS             9787..10434
                     /locus_tag="PK513_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOO81778.1"
                     /translation="MTQDLLNGFEDFVHYQYEDRTALMPKLVEDGQAPKYFIISCIDS
                     RANPGTIFRARPGTFFAHKAMGALVRPYKQGTALAASLQFALTYNNVPNVIVLGHTQC
                     GAIEALSKNLEDEEITSFISMAKASLKKAQACCSDHDEILAMTEKETILQSAENLKTY
                     PSVQRALAENRLNIKAWLFDMKEGQLLEYNIQNKDFRIISASHKTEDSRKEESHG"
     gene            10427..10954
                     /locus_tag="PK513_04695"
     CDS             10427..10954
                     /locus_tag="PK513_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOO81779.1"
                     /translation="MAERVNTLQTVIPVERIAAAIYLIRGEKVMLDSDLAELYGVTTG
                     ALNQAVSRNEERFPEDFSFQLTEIEWKSLISQTVISNTGRGGRRKLPRVFTEHGALML
                     SSVLRSQQAAQVSIAVTRAFVHMRRLLATNEELARKVAQHDQQIANLYEHVERLLRWP
                     EPEKKPIGYIKAKDA"
     gene            10994..11278
                     /locus_tag="PK513_04700"
     CDS             10994..11278
                     /locus_tag="PK513_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81780.1"
                     /translation="MTKKYWLIEGYDSLDRIYERKIPVGSMTDSQITNALKALAAKEG
                     LDLNEIIGAYAKRKTKVANDLLEVRRTSPFFGYECGENPYFIARVVEDNA"
     gene            11271..11837
                     /locus_tag="PK513_04705"
     CDS             11271..11837
                     /locus_tag="PK513_04705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81781.1"
                     /translation="MPKRTDINVRFAAPEDAEQMSAVANSNFVPLDKLSERAHQGFTI
                     WPRDQNDYANCIGRSSHVAVAEDADGKIVGHILAYDMGVFKQIARQSGSKNPIHQYKE
                     YPDTSIFLDQITIKPDHHGMGIGSMLDAFLRAHAPQSDLWVTDIVHKPVKNEASVAFF
                     ENLGFELQDEVEHDGWTLGIYEREMRHA"
     gene            11830..>14988
                     /gene="carB"
                     /locus_tag="PK513_04710"
     CDS             11830..>14988
                     /gene="carB"
                     /locus_tag="PK513_04710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOO81782.1"
                     /translation="MPKRTDIKSIAIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     VLINSNPATIMTDPEMADATYIEPITAKVVAKILRRERPDALLPTMGGQTALNTALEL
                     EKMGVLEELGIELIGAKADVIDKAENRERFKHCMESIGLENPKSAVVHSVEEAREALK
                     TLGLPTIIRPSFTLGGTGGGVAYNREEFEKIVREGLDASPTHEVLLDESLLGWKEYEM
                     EVVRDKNDNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQIMRNASIAVLRAIG
                     VETGGSNVQFGMNPDTGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LGNDITGGKTPASFEPTIDYVVTKIPRFAFEKFKGAEPHLTTAMKSVGEAMAIGRNFE
                     ESIQKAMRSLEKGLTGLEPVADMEIDEIRAEIARATPQRLLYVAEGLYQGLSIDEVYK
                     LCKFDKWYLERIDHLMKTERAMRENGLPTTAEGWMKIKKLGFSDTQLAKIMKVEEADV
                     RKARWAHDVHPVYKRVDTCAAEIPSDTPYMYGTYEGDGLSPAENEIDPCEKEKVIILG
                     GGPNRIGQGIEFDYCCVHAAYALKEAGYETIMVNCNPETVSTDYDTSDRLYFEPLTEE
                     DVIELIRAEQAFGRVKGVIVQLGGQTPLKLADALEREGIPILGTNPDAIDLAEDRERF
                     QKLVNDLGLKQPPNGLASSAEEAFEKAEGIGFPLVVRPSYVLGGQGMEIVHNMDELKR
                     YINIAVKVSGDSPVLLDRYLKSAIELDVDALCDGTDVYVSGIMEHIEEAGIHSGDSAC
                     VLPPHSLPSETVAELERQTIALAKALNVIGLMNVQFAVRPDDDTGELVIYLIEVNPRA
                     SRTVPFVAKATGVPVAKIAARLMAGEKLKDFKNILVGMPSTHSAVKAPVFPFNRFPGV
                     EPLLGPEMKSTGEVMGIDKNLAHAIAKSQLAAGTLLPKSGTVFLSVKTADKEALLPIS
                     QELEAMGFDLVATGGTCRYLDEHGVKVERINKVLEGQPHIVDAIINGDIHLVINTTMK
                     NAQAVADATSIRRQSLMHKVPYY"
BASE COUNT         3856 a         3593 c         3710 g         3829 t
ORIGIN      
        1 acagagaatg aacgtcggga gaatggagag aatctggtgg ctggagagag gttccagaaa
       61 gatgtcaaaa cgtccgaaac cctttgaata caaggggaaa aagaaaaccc gtctacccag
      121 aggattactc tggagagacg ggttgtcacc aaaaaaatgg tgggcctgga gggacttgaa
      181 cccccggcat aaccgttatg agcggtccgc tctaaccaac tgagctacag gcccccatga
      241 attttacgta cccatatacg tacccaaaga ggaaaaatga agtttttaaa taagacaaaa
      301 cccattgatt tacaacaatt aaatagcctt ccctctgtcg gcgaagctgt ccgttatgag
      361 cggactgctc taaccagctg agctacaggc ccatgcctta ttgtaacatg cgataattga
      421 ttaccagagt tcaaaacggg aggctactga aaaaagccgc cttttcggcc tatgcaaata
      481 ttattacata attaagtcgc gcgcatgcag aacggcaccc attgcacgct ggtcgttctt
      541 caccatgaga atagggtttc cgccagcact gagtgatatt tgcgcaacag ttgcccgctc
      601 cagtctacat aaagctgaat ttagagcatt gcgcatatcg gcctgaacaa tgttttttac
      661 atttaacaac ccaccttcga ccggcgtctt tactaaaaga ttaaaggcac catgaagatc
      721 attacatatt aaaagctgat gcggactgtt tcgcttataa cgtggcaaaa tatttataaa
      781 ccttgaatcg ccgccttctt tattttgtat attaaatgct atttgcaaga gtatatcaga
      841 taatcccggt ttttttgctc ctgcagacag atcataaaat ttgtcattat caagttcatc
      901 gacctttaac ggtgcaaaac tattttgcgc cgaaaaaaaa tctgcgaccg atgccggcag
      961 ctctgcgggc aaaaaaatgg cctgaggatc gacgttatgg tttgtggcat ctttcatgag
     1021 ccatatctca ttgtcaggaa tggtccactg ggcagcaggc gtctgagtta taaattttgc
     1081 tggaatttct ttcacgttgg ctctttttat ttccctcagg tcatccagat aataggatgg
     1141 cgccatgatt gccttttcaa cgtctctctt accttcagta taggtaagac ataccggatt
     1201 gccatactcg ttaaattcct tcatgagttc tgctttatgg caaaagctgc taatagaaat
     1261 gcgaggaaat gtgcgggcac cagacggcgt gatcaattca cagccctttg cctctagctc
     1321 tacccacggc caaacaggaa tgcctatatt tttactaaga tcatatgcaa tttcaggcga
     1381 caacctaacc tgaagtcgct cccattgact ggcgatcatc cccttggaaa gaaatacaaa
     1441 ccccttactt gcaatattat ctatcgtaac agctattacc tttgaggctc ttgccttttc
     1501 tccagtccag tcaacagaaa accttttttg gaaagatgtg aaagctactt cttccaaatt
     1561 atttcgccct gccaatgcta tgcctgttgc atagggcgcc gccacgccaa cagccctgcc
     1621 atcagaaaag actactttta acataccttt acgtatatcc tgacgggcta agcctgaggc
     1681 ttcgctacga aaatcgttaa gcgttgcaat gccaacaccc tttatatcct ggaacacctg
     1741 ctcatacatt ttttatgccc ttctattata gctctccggt tttgtcgggc aaaataacat
     1801 cagttatatg aaaaatcaat cttttcataa tgtttatttt taggcgaaaa aatggggctt
     1861 ttattcgcac ataaaaggca agaaaaggca gagactgcag aatttttaag tatccagaaa
     1921 gcttctcagc ttacgagagc ggcttgggtg tttgagcttg cgcagggctt tagcttcgat
     1981 ctggcggata cgctcgcgtg tcacgttaaa ttgctgcccg acttcttcca atgtatggtc
     2041 agtgttcatg ccaatgccaa aacgcatgcg caaaacacgc tcttcacgcg gagtcaacga
     2101 ggccagaaca cgggttgtgg tttcacgcag attggagtgg atagcactat caatcggtgc
     2161 gatagcgttt ttatcttcga taaagtcgcc cagagagcta tcctcctcat caccaatagg
     2221 ggtttcaaga gagaccggtt ctttggctat tttcaagacc ttacgaacct tgtctaaagg
     2281 catatgcaag cgctcggcca attcttccgg cgtgggttcg cggccaattt cgtgcatcat
     2341 ctggcgcgag gtccgcacga gtttattgat agtttcgatc atgtgtacag gaatgcggat
     2401 ggtgcgggcc tgatcagcga tgctgcgcgt gatggcctga cggatccacc aggttgcata
     2461 ggtggaaaat ttgtagccgc gccggtattc aaatttatca acggccttca tcagaccgat
     2521 attgccttcc tgaataagat caaggaattg cagaccgcgg tttgtgtatt ttttggcaat
     2581 cgaaatcacg agacgtaagt ttgcctcgac catctctttc ttggcgcgcg cggcttcttt
     2641 ttcgcctttt tgtacagtgc cgataatgcg acgccattcc gagatgggca gcattgcctc
     2701 ttcggaaatc agcgcgattt gctctcggat tttctcgatt tgctctttat tgttgctggt
     2761 aaacttatcc caacccttgc ctttaagctt ggatacgcgg gcaagccatt ttggatcgag
     2821 ttcggagccg taatagtttt ccaggaaatc ttcgcgctta accttggcgt caacagccat
     2881 gcgcattaat ttaccctcga cccccaccaa ctcgcggttc atagagtaaa ggcgatccaa
     2941 aagctgctca atccgtgcgt tattgagctg cacttcgttc atgtgctcaa ccatttcctc
     3001 tttgagttga ttgtattttt tgttgtcggc ttcggcaggc tccttaccat tttgcagcgc
     3061 atccagacga acctgttgca ccttttgcat tttcttatag gttttttgaa tctgcccaaa
     3121 catttcaaaa acttgcggag caagctcttc ttccattgcg gccaacgaga tatttacttc
     3181 atcatcgcca tcgctatcag aggattcgtc atcattttcg tcatcggaat cttcatcacg
     3241 gtcttcagcg tcctcgtcag attcatcttc ttccggctcc gcggttgcat cttccggagg
     3301 ttcattatag gtcgcatcaa ggtcaatgac ctcgcgtagc aagatatcgc cgtcttgcaa
     3361 cgctttatac caggcgataa tcgattcaat ggccagcggt gattcgcata tcccgccaat
     3421 cattaattcg cggccagcct caatgcgttt ggcaatggca atttcacctt cgcgggaaag
     3481 cagttcgacc gcgcccattt cacgcagata catgcgcaca gggtcatcgg tacggcccgt
     3541 atcatcatcg gcgatgtttc caccggcctc gtggcccctt tggttatcat caccagattc
     3601 ctcagcgaca tcgtcttctt tttcaacaag ctggataccg atgtccgaaa atgtcgccat
     3661 ggcatcatcg acctgctcgg aggaaaactc agcagcgggg atggctttgt tgatttcatc
     3721 ataggtcagg aagcctttgt ccttacctat tttgacaagt tttttcacga cattggcgag
     3781 tgagcctttg ccttctgtgt cctgattgtc gtcggtttgt ttttcactca cagccaaagt
     3841 tccaagtcct tttaaagcca cgtttttgtt tatccggcct ttgattaaat cttaatattc
     3901 ttgattttta aagtgtttag atacgatttt cacggaatta tacccgctat cgcctctatt
     3961 cagcgttctt gattcgtacc caatccctta acctatcctc ttgttcctca ttcgaagttt
     4021 caaacgccgt tttccattca gcgcacactt ctcctctggt ttcgcgccct tgtagcgcat
     4081 ggtacaggtc aagccagttt ttaacggctt ccccttgctc catggtttca aaagcgccga
     4141 tccgcgcaaa agcagcgtga acatatacag attcgtttaa tatgtcatgc atttcttgtt
     4201 cgaaaccgca ggaattcaaa tgggtactca aggacgctgt gtcaagagcg tcgtcgttgc
     4261 ctaaaaacac aataatctcc tgacgcaggc gctccatcgc gggatcgtta ataataaact
     4321 gaccaaactc ctcttccact ttgtgataaa gcgcagggtt attgatcatt gaagccaaca
     4381 agattcgctg tgaaagtacg ttttttttct gcaaaggcgg acgcaggcgt atcgaagatt
     4441 taggtttcgg ggcactgccc tgagagcgca ggccgccttt attttgatag ggccgaaaga
     4501 atttttccga aatgcgggca cggaccagtt cgcggtaatg gcgctggacg ttggcatcac
     4561 caatcacgga gacttgtttt tgcagcgctt tggtcaggcc agcgcgggct tccggggtat
     4621 cgagatcacg cccggcggta tgggtaatcc agaggaaatc aaacaaggag agcgaagcgc
     4681 tcagcacggc tttgaatccc cctgctccag aagcgcggag caagctgtcg gggtcttcgc
     4741 cttcgggcaa aaaggcaaag cgggcgcttt tgccgggctg aagcaatggc aaaagacgct
     4801 cacaagcgcg gctggcagcc ttgcgcccgg cattatcgcc gtcaaagcac aggatcggga
     4861 tcttatcctc atcgaccatc atcgaccaga ggacgagaat ctggtcttcg gtcaaagccg
     4921 tgcccatcgg cgcaaccgcg cctttgaagc catatttgtt acaggcgatg acatccatat
     4981 aaccttcagt gacgatcagt gtatgccctt cacgcgcggc ctgacgagcc atcgattcgg
     5041 cgtaaagcat acgcccctta tcgaacaggg gcgtatcggc ggtattgata tatttgggcg
     5101 gggtaaaatt tttgcctgtt tgtgggcgca tggattctgg caggatgcgc ccgccaaagg
     5161 caaccaccct gcctcggcgg tcacagacag ggaacatcac ccggtcgcgg aaaaagatat
     5221 aaggttctgc gccgcgattg gggtccttga gcaagccgac ctcaagcatt tgcgcgtcgg
     5281 aaaaaccctt ggctcttaaa tatttgcgca aagcctgacc atcagccggg gcatagccaa
     5341 tgcgaaaatt ggcgcgcgtt accttatcca tgccgcggcc ttcaagatat tcgagtatat
     5401 ctgtattttg cggctgtata acctgatctt gcatccatct cgtggcttca tccatcagat
     5461 cgtgcagccc cttggctttt tcggcctttt caacagccat cgggtcaggc ttgggcattt
     5521 gcatgccggc ctcggcgcac agcgtttcga ccgcgtcgat gaaggataca ttgtcgtggg
     5581 ccatgacaaa accaatgaca tctccatgtg caccgcagcc aaagcaatga taaaattgct
     5641 tgtcgtcatt aattgtgaag gacggtgttt tttcattatg aaacgggcag caggctttga
     5701 attcacggcc cgcgcgctgc acagaaatac gccgcccgat catttcggac agggtgagac
     5761 gattgcggag ttcatccaaa aaacgtggag aaatagacat gtgtttcacg ttagcagatt
     5821 ataaattcaa aaaatattat atttgcactt gctatatttc tggataatac atatacactt
     5881 gtctatattt tattcagaaa gggaagtttg gaactatgcc gaaagtcttt cagaaaaatt
     5941 gtaagattac actcattcaa tacggcgatt cctgtcaaaa tgcgctccat ctaaaagata
     6001 ttcaggctta tatttcaaag acttttaaga gatttataaa gtaaccgtct tctttcccca
     6061 ttttaaaatt ttgaatcata tcaatccctt atctatgtaa atttgcactg cagcactcat
     6121 tcgtgtatat tcggtgttag caatatttaa gaatgagagg atggcgtcat gccagaaata
     6181 ttttcaacag gtgccaagtt caagctggtt gagtatggtt caaaccaaca aggtctggtc
     6241 agtatcgcat acgtcaaaga caagctggtt gaactgctcc agcggattgc gcaaaaataa
     6301 caacacttaa aagataacag ggccgcataa tgcagccctt ttctttgcag tacataccac
     6361 cggttttaac ttaccccagc ctggctttta caattgctcc ggccttaccc atatcaatct
     6421 gcccagcata ttgggacttc aaagcgccca taaccttacc catatccttg attccagttg
     6481 cgcctgtgtc accgatgatt ttttctaccg ctttggcaac ttcctcttca ccaagctgca
     6541 ccggcatgaa tttttgaatc acttcaattt cagcttcttc gcgctcggcg agttcttcac
     6601 ggcctgcgtc acaatatatt ttgctggctt cctgacgctg tttgatcatg ctttgcaaca
     6661 tcgagagaat ctcactttct tcaattccat cagcctttcc ttgccctcgc gcagtaatat
     6721 cacgatcctt gagcgcagcc agaatcagac ggatggttga catcgccgtt tgatccttgg
     6781 ctttcaatgc atctttcagc gcggcagtaa attcttccct tttctccatt ttatcctcat
     6841 ctttcgtcgt tttttgcttg gcattcgccg gtaaattctt tagatacttt gtttctagcg
     6901 acacccaagc tagaaagaca atcttttatg acgcaagtac ctaccctaaa acagccttcc
     6961 gaagcaagcg ctgtgattct ttttgctgac ggcacgtcat ttttcggacg cgggtttggc
     7021 gcacaaacca aagcagtcgg cgagatttgt tttaatactg caatgacggg gtatcaggaa
     7081 acactgagcg accccagcta tgccgggcag attatcacct tcacattccc tcatatcggg
     7141 aatgtcggca ccaacaatga agatttggaa agccttaacc cggcggcgcg cgggctgatt
     7201 gtgcgtgagg acgtaaccaa tccttcgaac tggcgggcgc agagccgtct tgatgaatgg
     7261 cttaagagcc ataacctgcc ggggatcagt ggtatcgaca cacgcgcgct gacccgttat
     7321 atccgcgata atggtgcgcc gaacagtgtg ctggttcatg atctgtcggg caaattcgat
     7381 ctagaggctc tgcataaaga agctcaggat tggcccgggc ttgaaggaat ggatctggcg
     7441 aaagaggtgt cttgcacgca aagttatagc tggaaggaaa aatgctggac acttgaggag
     7501 ggttatacag aacaagatca tcctgagtat aaagtcgtgg ccgttgattt tggagccaag
     7561 cgcaatattc tgcgctgcct tgctgataca ggttttgatg tgatggttgt tcccgcaaca
     7621 agcacggcgg aagagatttt agcacataaa ccggatggcg tgtttctgtc caacggtccg
     7681 ggcgatccgg cggcgacggg tgaatatgcc gtgccgatga tccgcgcttt gctggaccaa
     7741 ggaatgccga ttttcgggat ttgtctgggc catcaattgc tggcgctggc cttgggtgca
     7801 aaaacccgca agatgcatct ggggcaccgc ggggcgaacc agccggttaa agatttgctc
     7861 accggcaagg tcgagattac ctcgcaaaat catgggtttg aggtgattgc cgagtcgctg
     7921 cctgagggga cagaacagag tcatctcagc ctgttcgacg gctcgaatga aggtttaaga
     7981 gtgaaggata aacctgcttt ttcggtgcaa tatcaccccg aggcgtcgcc ggggccacaa
     8041 gacagttatt atttgtttga acggtttaaa gagaatattc agaaagcgaa ggccgcttga
     8101 caaaaagctg gacacgaaaa taaagttatg gcaatttctt gcgaattttc cgaatgaaag
     8161 caaactggaa accattgtag ccgcttaaaa ctaatcaaca gggcccttaa aactacggaa
     8221 agacacttta tatgagtcaa attacatccc aagaaaccgt atccgttgat gaccttagtc
     8281 aaggtatgac tgctcccggt aaagtaaacc attttctaga aggttatttc aggcacacag
     8341 atatagaccg tattccagag aaattaatgt tcatcatagc ctgcgccgat tcccgcgaag
     8401 atatggaggt caagtttggt cttaattatg gcgaagcctt attctacagg gccgtcggcg
     8461 cggtggtccc cgatcctgat gatgaaatag cctctcttcc gatgaaggct gcacttgaac
     8521 acgctcttaa aaatcctcat caggttgaaa ttgcccttct tattccgcat acccattgcg
     8581 gggctgtaaa agcgcatatg acgggagaag ggccaaaggc gctcttaacg gcgatgagcg
     8641 gcactacaaa tttcagcggt atttcgggca gcataatgac accggaattt aaacttgttt
     8701 ccgatcctga cgaggtcatg gcggttttaa gacaagaaaa tatgaatgaa ggagtaaagc
     8761 atgccgtgtt acgaagcgca gaaagagcct gcatggatgt aagccgtgac aatttattaa
     8821 aataccccca gatcaaagaa ggtctgaaga gcggaaaatt taagctttta acagggcttg
     8881 atgatatcgc ctgcgacaat atctcttttc agatggatgg gcacagtgaa ggaaagaata
     8941 ttaccgagag attccttcac cgatataaaa atgccacaga aggattatac gattctgaaa
     9001 acggtggtcg catgcccgaa cttgttttga acgggcagca ccccgaagcc ctttttatca
     9061 gcggtctgag cggagatctt ggcaaagtct tcagcgttcg catcggcgat gccttcgtcc
     9121 acagacatac tttcgggttg atccctcccc caagacagga cacatcacag ccggacccgc
     9181 tggctgccac gattgctttg gctttgatca gcggcgtaga gaccttcatc cataaatttc
     9241 atgcggacgg cacctttaga aactatatgg atggagatct tctgccttac tcctctcaat
     9301 ggcttgagta ctgcattccg gatttatcac agatgaaaaa ttcgggagca tctttggatc
     9361 aggtgcgcct gcactgtatg ctcaaaagca ttgaacattt aaaactctat gacggtgttc
     9421 aagcggctat gaacgccagg aagctttcgg ttttatctct ttacgaagat cctgaaaaaa
     9481 gggagctttt cgttcccgat agggataaaa attgttttgt taaagtcctg ctgccagatg
     9541 aaaatcctgc cccgcaagag aatcgagctt ttgagctggc gtaatggcgc gcaggaaaag
     9601 tacttacata tttttctgtt gaactcttcg cgcaattaag atacacaatc atcctcggta
     9661 ccactaacaa aaccgagacc tcatgtccaa gcgcgctgac atcacgtcca tctgcatctg
     9721 cattaccggc cctcatgttt ctccgtttga gcggttcaga gaaaatattc agaaggccaa
     9781 ggtcgcatga cgcaagactt gctaaacgga tttgaagatt tcgtccatta tcaatatgag
     9841 gataggacgg ctttgatgcc taaactggtt gaggatggtc aggccccgaa atatttcatc
     9901 atcagttgta tcgattcgcg cgccaatcca ggcacaattt tcagagcccg tccgggcact
     9961 ttttttgcgc ataaggcgat gggcgctctt gtgcgcccat acaagcaagg cacggcgctg
    10021 gccgcctctt tgcagtttgc gcttacatac aataacgtcc cgaacgttat cgttttaggg
    10081 catacgcagt gcggtgccat tgaagcgcta tcgaaaaatc ttgaagatga ggaaattacg
    10141 agcttcatca gcatggccaa agcctcactc aaaaaagcac aggcctgttg ttcggatcat
    10201 gatgaaatat tggccatgac cgaaaaggaa accatcctgc aaagtgcgga aaatctaaaa
    10261 acctatccca gcgttcaacg cgctttggcc gaaaaccgcc tgaacatcaa ggcatggctg
    10321 tttgatatga aagaaggaca attgctcgaa tacaatattc agaataagga ttttagaatt
    10381 atttcggcct ctcataaaac tgaagacagt cgaaaagagg aaagccatgg ctgagcgcgt
    10441 gaacaccctt caaactgtca tccccgtgga acggatcgcg gcggcgattt accttatccg
    10501 gggggagaaa gtgatgctcg attccgatct ggcggaattg tacggggtaa cgaccggtgc
    10561 tttaaatcag gccgttagcc ggaatgaaga acgctttcct gaggattttt ctttccaact
    10621 cactgaaatt gaatggaaat ctttgatatc acaaactgtg atatcaaaca caggccgcgg
    10681 tgggcgtcgt aaattacccc gcgtttttac tgaacatggc gctttgatgc tttccagtgt
    10741 tttacgcagc caacaagcag cgcaggttag catcgccgtt acaagggctt ttgtccatat
    10801 gcgcagattg ctggcgacaa atgaggaatt ggcccggaaa gtggcgcagc acgatcagca
    10861 gatcgcgaat ttatatgaac atgtggagcg gcttttgaga tggccagagc cggagaaaaa
    10921 accgatcggg tatattaagg cgaaagatgc gtgatatttt gaaccgcaga gacgcagaga
    10981 gcgcggagga aagatgacaa aaaaatactg gttaatagag ggctatgata gcctagatag
    11041 aatatacgaa agaaaaatac cggtaggtag tatgacagac agtcaaatta ccaatgcttt
    11101 aaaagcattg gcagctaaag aaggcttaga cctcaatgaa attattggag cttacgcaaa
    11161 aaggaaaaca aaagttgcta atgacctttt agaagttcgc agaacctctc cgttttttgg
    11221 atatgaatgt ggagaaaacc cttattttat tgcgagagta gtagaagaca atgcctaaaa
    11281 gaactgacat aaacgtcagg tttgccgctc cggaggatgc ggagcagatg agcgctgttg
    11341 cgaacagtaa ttttgtccct ttggacaaat tatccgaacg tgcgcatcaa ggctttacaa
    11401 tctggccaag agatcaaaac gactatgcaa attgcattgg ccgctcttct cacgtggcgg
    11461 ttgccgagga tgccgacggt aaaattgtcg gtcatattct ggcgtatgat atgggagtct
    11521 ttaagcaaat agcccgccag tccggaagta aaaaccccat acaccaatac aaagaatatc
    11581 ccgatacttc cattttttta gatcagatta caatcaaacc cgatcatcat ggaatgggaa
    11641 ttggttcgat gcttgatgcg tttttgcgtg cgcatgctcc gcaaagcgat ttatgggtta
    11701 ctgacattgt gcataaacca gtcaagaatg aagcgtctgt cgcctttttt gagaatttag
    11761 gatttgaact acaggatgag gtcgagcacg acggctggac tttgggaatt tatgaaagag
    11821 aaatgagaca tgcctaaaag aaccgacata aaatccatcg ctattatcgg ggccgggccg
    11881 attgttatcg ggcaggcctg cgagtttgac tattccggcg cacaagcttg taaagcgctg
    11941 cgtgaggaag gatatcgggt ggtgctgatc aactccaacc cggcgacgat catgaccgac
    12001 ccggagatgg ccgatgcgac ctatatcgag ccgattaccg ccaaagttgt cgccaagatc
    12061 ctgcgcaggg aacgtccgga tgcgttgctg ccgactatgg gtggacaaac ggcgctcaac
    12121 accgctctgg agctggaaaa gatgggtgtg ctcgaagagc tgggcattga gctgatcggt
    12181 gcgaaggcgg atgtgatcga caaggccgaa aaccgcgagc ggtttaagca ctgcatggaa
    12241 agtatcgggc tggaaaaccc gaaaagcgcg gtggtgcatt ccgtggagga agcgcgtgaa
    12301 gcgcttaaga cccttggtct gccgacgatt atccgcccct ccttcactct tggcggcacg
    12361 gggggcggcg tggcctataa ccgcgaggaa tttgaaaaga ttgtgcgcga ggggctggac
    12421 gcttcgccga cccatgaagt gctgcttgat gagagccttt tgggctggaa agagtatgag
    12481 atggaggtgg tgcgcgataa aaatgataac tgcatcatcg tgtgttccat cgaaaatatc
    12541 gatccgatgg gcgtgcatac cggggattcg atcacggtgg ccccggccct gacgctgacc
    12601 gataaagaat accagatcat gcgcaatgcc agcatcgctg ttctgcgcgc aattggcgtt
    12661 gagacgggcg gatcgaacgt gcagttcggt atgaaccccg atacaggccg catggtggtg
    12721 atcgagatga atccgcgcgt ctcgcgttct tcggcgctgg cctctaaagc cacaggcttt
    12781 ccgattgcga aaatcgcggc gaagctggcc gtcggctata cgctcgatga gctgggcaat
    12841 gacattaccg gcgggaaaac cccggcctcg tttgagccga caatcgacta tgtcgtcaca
    12901 aaaattccgc gctttgcgtt tgagaaattc aagggcgccg aaccgcacct gaccacagcg
    12961 atgaagtcgg tgggtgaagc gatggcgatt ggccgtaatt ttgaggaaag tattcagaaa
    13021 gccatgcgca gccttgaaaa aggactgaca ggccttgaac cggtggcgga catggagatt
    13081 gatgaaatcc gcgccgagat tgcccgcgcc acgccgcaac gccttcttta cgtggccgag
    13141 ggcctgtatc aaggtctgag tatcgatgaa gtttacaagc tttgcaaatt cgacaaatgg
    13201 tatctggaac ggatcgatca tttgatgaag accgaacgcg cgatgcgtga aaacggtctg
    13261 ccaacaacgg ctgaaggctg gatgaagatc aagaaactcg gtttttcaga tactcagttg
    13321 gccaagatta tgaaggtcga agaagccgat gtgcgcaagg cgcgctgggc gcatgacgtt
    13381 catccggttt acaaacgcgt ggatacctgc gcggcggaaa tcccgtcgga tacgccatat
    13441 atgtatggca cctacgaagg tgatgggctt agcccggctg aaaatgaaat tgatccttgt
    13501 gagaaagaaa aagtcattat tctgggcggc gggccgaacc gcatcggtca gggtatcgaa
    13561 tttgattatt gctgtgtgca tgcggcctat gctctgaaag aggcgggtta tgaaacgatc
    13621 atggtcaact gcaacccgga aacggtttca accgactacg acacctcgga ccggctatat
    13681 ttcgaaccgc tcaccgaaga agatgtgatt gaattaatcc gcgccgagca agcgttcggt
    13741 cgggttaagg gcgtgatcgt ccagcttggc ggtcagacac cgctcaagct tgccgatgca
    13801 ttagagcgtg aaggcattcc tattctcggg accaacccag acgcgattga tctggctgaa
    13861 gatcgcgaac gttttcaaaa gctggtcaac gacctcggcc tcaaacagcc gcccaacggg
    13921 ctggcctctt ctgcggaaga agcctttgaa aaggccgaag ggatcggctt cccgctcgtt
    13981 gtacgcccct cttacgtgct aggcgggcaa ggtatggaaa tcgtgcataa catggatgag
    14041 ttgaagcgct atattaatat tgcagtgaag gtttcgggcg attctcctgt tttgctcgac
    14101 cgttatctca aaagcgcgat tgaattggat gttgatgcgc tgtgtgatgg gacggatgtg
    14161 tatgtctcag gcatcatgga acatatcgaa gaagcgggca ttcactcggg cgatagtgcc
    14221 tgcgtgttgc cgccgcattc cctgccctct gaaaccgtgg cggagttgga acgccagacc
    14281 atcgcgctgg ccaaagcgct gaatgttata ggcctgatga acgtgcaatt cgcggtgcgt
    14341 ccggatgatg ataccggtga actggtgatt tatctgattg aggtcaatcc gcgtgccagc
    14401 cgtacggtgc cgtttgttgc caaggcgacc ggcgtcccgg tggctaaaat cgctgcacgc
    14461 ctgatggccg gtgaaaagct taaagatttt aagaatatcc ttgtcggtat gccgagtacg
    14521 cacagcgcgg taaaggctcc agtttttccg ttcaaccgtt tccccggtgt tgagcccctg
    14581 ctcggccctg agatgaaatc caccggtgag gtgatgggta ttgataaaaa tctggcgcat
    14641 gcgattgcga aatctcagct cgcggcgggc acattgttgc ccaaatccgg gacggtgttc
    14701 ctgtcggtga aaaccgccga taaggaggcg ttactgccga tttctcagga gctagaggcg
    14761 atgggctttg atctggtcgc cacgggcgga acgtgccgtt atctggatga acacggcgtg
    14821 aaggtcgaac ggattaacaa ggttctggaa ggccagccgc acattgtcga tgcgatcatt
    14881 aacggcgaca ttcatctggt gattaatacg accatgaaaa atgcgcaggc cgtcgcggac
    14941 gccacttcca tccgccgcca gtccctgatg cacaaagtgc cgtattat
//
LOCUS       DAOSWR010000043        14822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2552_length_14822_cov_5.221846, whole genome shotgun sequence.
ACCESSION   DAOSWR010000043 DAOSWR010000000
VERSION     DAOSWR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2552_length_14822_cov_5.221846"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="hemC"
                     /locus_tag="PK513_04715"
     CDS             <1..670
                     /gene="hemC"
                     /locus_tag="PK513_04715"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOO81783.1"
                     /translation="LFSRKIKELADYSQLKNEGLAVLPQAAVVGSASVRRGAFLLSAR
                     PDLKIVPFRGNVQTRINKLRGPESGGVIDCTLLAMAGLNRLGLASEVDIILEPEEMLS
                     AAAQGAVGIEVRASDNDILSIFSHISDYNTVVCVKSEREVLRILDGSCHTPIGVYGVL
                     DDGELWLRVRVVSLDGAQRFDDEIRGAVQTVEEGQSLGRVLGERLKRRVPPEIFNQEI
                     VDDG"
     gene            663..3167
                     /locus_tag="PK513_04720"
     CDS             663..3167
                     /locus_tag="PK513_04720"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF021264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOO81784.1"
                     /translation="MAEKILQVSSQQANGQRSVILVTRPKTQAGLFADDLAGRGYEAF
                     VEPMSIIEPLDFHVPDLGYYQGLIFTSANALEIYAQAHKDRSLPVYVVGRQTQEMAYE
                     HGFKKVYSARGNAANLADYIKGHVPDMKCPLLHVRSTHVAYPLKEALQNTGYNVGQLI
                     VYDAPMVEAFSDEGCAFLENGRVQAVTFFSRRTAENFLKLIEGAGLTEVLAGIKALCL
                     SKSVLQCVRPELWQGAYVADKPERQGMMDLVARYCVKGPEKLQTDKDDVERQGMSKSE
                     NRPIENAAEVIERFGGIRPMAKKIDVAVTTIQGWKKRDVIPAARRQVILEAAVAHDVD
                     LSDILPDAPPANENNKGEPAERKAARDDAIVLEQKLVSDLQAESSDGEAEEGKLTMTT
                     ERRSVLAASAAMQGNMAQKEKHVLSRHSWLIIVIILGVTIGVAALLWPQKIDNNGRPD
                     RLSALEARMQETEGEMAAVKDRQSFFGTLIPDNLDEQLASLQDQAGRAKQQLGQAVEK
                     VQEVSADVLAENAGTLSERAEKLENHLQEMTGSPVLAGMLEKLQEMQTDTEGKNQLDQ
                     AMEELSGIIGGLEGQLGAGSGLFESTLDSARGQSEALGQTFENVPATDLKAAAMLLAM
                     TQFRSSLNRDNAAFGNDFNVLMGLVGEEDTELRSALERLAPHAQSGVLTPSGLSNEFR
                     SLAGEAVVASLQGEDVSLGERAKARINQVLQVEKDGELISGTETQAKLAAADQLLQEG
                     NLEEAIATVETLDGPAGLYMAEWLDKANVTLMTEKLKGMLTQSVNTHAYGKALGITAG
                     GVIPGSSKLIRNEETGINILRRNTLPKASDISNPFE"
     gene            3199..4581
                     /locus_tag="PK513_04725"
     CDS             3199..4581
                     /locus_tag="PK513_04725"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOO81785.1"
                     /translation="MIKALWFAIKVGVLIALSVWIAERPGSVRIEWMEYTFTIQMGFF
                     LLVALVFVLLTLFIYQVLKTFVGFPSSLRRYNEVKAREKGYRALTRGLTAVAAGDKKA
                     AALYSKKASKLLEGDTGLPLLLEAQAARLDGREEDAAQSFVALLEDKDASFLGVRGLL
                     QTALGSGDDEGALALAEKALDLHPHQGWILKIVYDLQIRQRLWTKAEKMLARVENAGV
                     MAPEVVRSDRLALYLAQAEKDLADGYSSDALYLIKKAQKLSPDFAPATISLAEFHMRE
                     GHPRKAQNAVLKAWKTAPHEALALYWMTLKDDENVPDALARLRHLERLLKMCGESARV
                     QLMAGQIAIEAGLWGEARAFLQEAEKLGATTSLYKAFAMLEERSSRNEVAIKEHLSRA
                     AGAMADKVWVCRETGNIYERWSPVAQPHGSFNTIIWDIPHAHYGPARLLNDKAHMSEA
                     LIEAPDIDAA"
     gene            complement(4578..5381)
                     /locus_tag="PK513_04730"
     CDS             complement(4578..5381)
                     /locus_tag="PK513_04730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO81786.1"
                     /translation="MSTNGNGNNRDKDPDDKKIIKFPSLAERDKMCREQHEEEQRWRK
                     QYRQKSKAAHKANTQPFFKAGNIPPFSRALIASFWIVQLSLYLLISAPDRYEIFQTFG
                     FVPANYTNGAPWPWTTFISPVTHAFIHGSWMHLFFNSVMGLVLCMFFEKIFGARTTAI
                     FFTLCILAGAALYFILSPFSTLPVIGASGGISGLFAAVIYISMTQTGNPLAKRFSKHG
                     PWPILAFWALLIIIPGLLIGQSVAWQAHLGGYLAGISLLIGMKKGRIKL"
     gene            complement(5500..6165)
                     /locus_tag="PK513_04735"
     CDS             complement(5500..6165)
                     /locus_tag="PK513_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81787.1"
                     /translation="MQVNVKQFLEEAGITEAFYPGKRLVHSCRQPGEYKSHCVVLDWR
                     NPDKIKIEVKAGLSGKDLEPKQLKYYPVSFQTPTYVDIEIINDNKDDKDDEGEKGKSG
                     KSGGGGKKPAKKKLSDVKLMASEAFGTVMEGKIPEAGKIIEMVVLGTQIAAEAYSNVM
                     GKLAHGIAHAKISTTDMLAQAGKFVTKYTPPSFMKPTGDEQATYKYDREKNADIGYKA
                     GLG"
     gene            6419..6991
                     /locus_tag="PK513_04740"
     CDS             6419..6991
                     /locus_tag="PK513_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81788.1"
                     /translation="MSIVFLGVGFGPALAQELFLRKDDGSAKDGKPTLYMKPRESGFT
                     DQTEHFAVKYKDKLKLQQQKGNTKVLISSFEEMQELGFKPRNAKEIDAYGRMHRALSQ
                     NIMYKRREALIAHLEKQEREVEAMLARRDAGALKVDDQKDSATVSKETSEDAQGGHDT
                     QTTKILKKPRVYVKKTADEKARPTKVFKDY"
     gene            7077..7907
                     /locus_tag="PK513_04745"
     CDS             7077..7907
                     /locus_tag="PK513_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO81789.1"
                     /translation="MDVFAEFAVQNWAVLIGGLVVLGALAGFLAGLLGIGGGIVLVPG
                     LLFIFSALGLASESLIHVCVGTSLALIVPNGLMSSRAHWQKGAVDFSLVKPIGVGVLV
                     GVVIGTLIADRLEGVQLQQVFSVVIVCLAAVMAINPARFHFFKTMPPQPWPSIAGSFI
                     GAISSLIGIGGATLSVPFMSLCGVDMRRAIGSAAALGVVIAVPGTFGFILTGLGEPGR
                     PPFSIGYVNMLAWITILPSSLLAVRAGVWAAHKLPLEVMRRVFAGFLVLVALKMGLGL
                     "
     gene            7904..9181
                     /locus_tag="PK513_04750"
     CDS             7904..9181
                     /locus_tag="PK513_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO81790.1"
                     /translation="MSAVITHSDTGVIHRLMRVDTRYFQGGMLCLAILIYGAWGSPTP
                     DSPGWLELLIAALLAASVGISGLMGAFIFRTKDPRWKQTGQILLIFGLSVPLLGGLWG
                     GYERGFIIRDLAAFGFMVLPVFLWPLIQVQANMARPFVYAAAALGILFALRASFDVPS
                     LAVGEELFYLANMPSVLFAAVFLGGTAMQIFVRDCTLRAVLVAFALLAFVGVSLWSMI
                     EMQQRASLGVFVLSIVCVYGLCLWRNARRSFVLVCFLVILVVFLWPEVGNVLNDMERK
                     TALVGGNMRVEEWRAVWEEISIHPLSLFFGQGWGATFSSPAVADIRVNYTHGLLSSLL
                     FKTGLVGFSLGGVYLLALARTGLRLFAHAPVLALALAGPILIDVFLYASFKSLDFGLM
                     LALVVSCCFYGDGKVCGVETDADVLYSGKVIKG"
     gene            9249..10445
                     /locus_tag="PK513_04755"
     CDS             9249..10445
                     /locus_tag="PK513_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO81791.1"
                     /translation="MYPPVRGASGRVLRDLARSFAREGWQVTVITTGPKAMSERDGAV
                     RVVRVKGAEKPGGAFGYIWIWMKLLWVALRLPKTHLVVTLTDPPFLAIVGRILQFFKR
                     SHHIHWCQDLYPDVFSALGVKFPEFIMRFLKNRVRAAMKRADKVIVIGRCMARHLNYD
                     GFDPKQITVIPNWVDSELVQPLQDDDCEPVYVTTPESYRSYEEQRKHGPKFRVLYAGN
                     IGRAHPVETILKAAEILNETNPDVEFVFVGDGPRHEIINRKRILMHLENIRLLPYQPV
                     SRLKDLMESGDMHLISMDGQAGGLLVPVKLYSALAAHRPCVFIGPAHSEAAKVIEDFK
                     AGVVCAPGDVQGLAEHIRRYRENSDKWFSDFEGAKAASEVFVPKESINAWIERAWAVV
                     DQDVHV"
     gene            10442..11455
                     /locus_tag="PK513_04760"
     CDS             10442..11455
                     /locus_tag="PK513_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81792.1"
                     /translation="MSQDRYDSEVTPSNREQTLGEFMREVWSGRVFVFGGLMIGLVLA
                     FVMMSSAVPHFRAEMILAPASPMNMAASPVNYAGGALEQNSEVMSFTRFEVSFKGVPP
                     AALLLRDPEITDGLARDKAFSFSRPEQGWSAEKLAEYIARRVEVDPVGETSLRALRYL
                     HPDKEFAALFLQRLHNITDGLIRHGIRREVNERITYLNKAIAENMNPEHRRALTALLM
                     EQERLKMLVSIDQPYAASIVVPASVSVKARWPDGSLIYSGFAFVGALLGFVVFSARGV
                     CREDIFAVFPGGKVKQQEWFFPESGNNNEKPSFAAGRRPLTGKKRDQDPDEDSDLPSE
                     AAE"
     gene            11465..12325
                     /locus_tag="PK513_04765"
     CDS             11465..12325
                     /locus_tag="PK513_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO81793.1"
                     /translation="MGNPPVSVIITTKNEERRIGACLEVLSRFDDVWVVDSDSSDQTQ
                     QIARKAGARIKNYVWDGCYPKKRQWCLENLALKYEWVFFVDADEIVPRGLVDEIAGLD
                     WCAAGYFVKGLYEFEGRFLHHGLCNNKLALLDRRKVEFPVVDDLDIEGMGEIEGHYQP
                     VLKNGFENEDIGQTETPLLHYAYDDLQSWEERHHRYARWEAGMNAKGAWPVDPRPVRQ
                     FAKEAFRNVPFRGVVAFVHCYIFKLGFLDGAAGYRFARSRARYYRMISDASKAMVQPC
                     AESKEQPGRL"
     gene            complement(12657..12998)
                     /locus_tag="PK513_04770"
                     /pseudo
     CDS             complement(12657..12998)
                     /locus_tag="PK513_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            13120..14523
                     /locus_tag="PK513_04775"
     CDS             13120..14523
                     /locus_tag="PK513_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81794.1"
                     /translation="MAERAIDTGIAKALAQQFLKRLVDDFKFGSDVDTMEQVVNGLLP
                     RDDKGKPKFDDASRAQAAKTLITGLNALYKKSDVDSRVQGAIENIFKDARLKTAHDLA
                     TQLKDQMRTQEFLALLKNDDADFGFYFNDRQIKAIKHNLNLISPGQNGGPGLGDMIAN
                     FLGLRGDLDGTAEPTARSELTEGFHAMTNGGQNSLAKFWDKDRAGDNKAFNVWNPPSG
                     EEQKKAFFSKTGMMNSKGKSEYQHYDSDAKVFKINFEDMILAKWEKDGIVKFNGPYAR
                     EHFINFVQNQRMSGELGAPLSAALESKGQISFASGSARADFDEFTSGLAGQGLDNYEI
                     AEAIKKYAEDSTNGVTLPKAGLHKFENELPETISLFQDAKSPDEFADKVLAFANWYPG
                     SGIVVVDPKVEQETPSVMRGKPNDAPAHSKASASKTPEEKLGEIKVVAGQITTPSASP
                     EPPAAVPVISSPGVQPS"
     gene            complement(14532..>14822)
                     /locus_tag="PK513_04780"
     CDS             complement(14532..>14822)
                     /locus_tag="PK513_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HOO81795.1"
                     /translation="QFRERKASLDYIHEAGGFTFHADKDRTFVIYPDGAAQPLQVSSW
                     NYNPVMIPPGSTIVVPRDPKPFDFIQSAKDVSQILSNLAVTAIFIDDVADSN"
BASE COUNT         3300 a         2717 c         4544 g         4261 t
ORIGIN      
        1 attattttca cgtaaaataa aagaattagc tgattatagc caattaaaaa atgaaggttt
       61 ggcggtgtta ccgcaggctg ccgtcgtggg cagtgcgagc gtgcggcgcg gggcgttttt
      121 gctttcggcg cggcctgatt tgaagatcgt gccgtttcgc ggcaatgtac agacgcggat
      181 taataaattg cgtgggccag aaagtggtgg ggtgattgat tgtacgcttt tggcgatggc
      241 gggattaaat cgccttgggt tggctagtga ggtggatatt attctggagc cagaggagat
      301 gttgtcggcg gcggcgcagg gtgctgtcgg gattgaggtg cgggcgtccg ataatgatat
      361 tttatccatt tttagccata tttctgatta taatacagtg gtttgcgtaa aatcagagcg
      421 ggaggttttg cggattctgg acgggtcttg tcatacgccg atcggggtgt atggggtgct
      481 ggatgatggt gaattgtggt tacgggtccg tgtggtctca ttggacgggg cgcagcgttt
      541 cgacgatgaa attcgcggag ctgtgcagac ggttgaagag gggcaatcgc tcgggcgtgt
      601 gcttggtgag cgtctaaaac gccgtgtgcc gccagagatt tttaatcaag agattgtcga
      661 cgatggctga gaaaattctt caggtgagca gccaacaggc gaacggccaa cgttcggtga
      721 ttttggtaac ccggcccaaa acacaggccg ggctttttgc cgatgatttg gcagggagag
      781 ggtatgaggc ttttgttgag ccgatgagca tcatcgagcc gctcgacttt catgtgccgg
      841 atttgggtta ttatcagggg ctgattttta ccagcgcgaa tgctctagaa atatatgcgc
      901 aggcccataa agaccgcagc cttccggttt atgttgttgg gcggcagaca caggaaatgg
      961 cctatgagca tgggtttaag aaagtttaca gcgctcgggg caatgctgcc aatctggcgg
     1021 attatatcaa agggcatgtg ccggatatga agtgtccgct tttgcatgtg cgttccacgc
     1081 atgtcgccta tccgttaaaa gaggcactgc agaatacggg atataatgtc ggtcagttga
     1141 ttgtttatga tgcgccgatg gttgaagcgt ttagcgatga agggtgtgcg tttttagaaa
     1201 atggccgtgt tcaggcggtg acattttttt ccaggcgtac ggctgaaaat tttcttaaac
     1261 ttatagaggg cgcgggttta accgaagttc tcgccggtat aaaagccttg tgcctgagca
     1321 aatccgtgtt acagtgcgta cgccctgaat tgtggcaagg tgcttatgtt gcagataagc
     1381 ctgaacgtca gggcatgatg gatctggttg cgcggtattg cgtgaaaggg ccagaaaaat
     1441 tgcagactga taaggatgat gtggagagac aaggtatgag caaatccgaa aatagaccga
     1501 ttgagaatgc ggcggaagtg attgagcgtt ttggcggcat tcgccctatg gccaagaaga
     1561 ttgatgtggc tgtgactaca attcaggggt ggaaaaagcg ggatgttatt ccggcggctc
     1621 gcaggcaggt tattttagag gcggcggtgg ctcatgatgt tgatttgagc gacattcttc
     1681 ctgatgcgcc gccagcgaat gagaataata agggagaacc tgctgagcgt aaagctgcga
     1741 gagacgatgc aatcgttttg gagcagaagc ttgtttctga tttacaggca gagtcttcag
     1801 atggagaggc agaggaaggt aagttgacga tgactacaga gcggcgttct gttttggcgg
     1861 cgtctgcagc gatgcaaggc aatatggctc agaaagaaaa gcatgttttg tcacgtcata
     1921 gctggttgat tattgttatc attttggggg taactattgg agtggctgca cttctttggc
     1981 cgcagaaaat tgataataac ggcaggccgg atcgtttaag cgctttggag gcgcggatgc
     2041 aggaaactga aggtgaaatg gcggcggtta aagatcgaca atcgtttttt ggtacgttga
     2101 ttccggacaa tcttgatgaa cagcttgctt ctttgcagga tcaggccggg cgggccaaac
     2161 agcagttggg gcaagcggtg gaaaaggtgc aggaagtttc cgccgatgtt ttggctgaaa
     2221 atgctggcac tttgagtgaa cgtgcggaaa agttggaaaa tcatttgcag gagatgacag
     2281 gatcgccggt attggctggt atgctggaaa agctgcaaga gatgcaaact gatacggagg
     2341 ggaaaaacca gcttgatcaa gcgatggagg agttgagtgg cattataggg gggctggaag
     2401 ggcagcttgg cgctggatcc ggtttgtttg aaagtacgct cgattctgcg cgtggacaaa
     2461 gtgaggcact gggccagacg tttgaaaatg tgccagctac tgatctgaag gcggcggcaa
     2521 tgcttttggc tatgacgcag ttccgttcgt ccttgaatcg ggataatgcg gcctttggca
     2581 atgattttaa tgtgctgatg gggttggttg gtgaagagga tacggagctt cgctcagcgc
     2641 ttgagaggct tgcgccgcat gcgcaaagcg gagttcttac accgtctgga ttaagtaatg
     2701 agtttagatc gttggcaggt gaggctgttg tggcttcgtt gcagggcgag gatgtgtctt
     2761 tgggagagcg tgcgaaggcg cggataaatc aggttttgca ggttgaaaaa gacggcgaat
     2821 tgatttccgg tactgaaacg caggctaagc ttgcagcggc ggatcagctt ctccaagagg
     2881 gtaatctgga agaagctatt gcaacggttg aaacgttaga tgggcctgcg gggctgtata
     2941 tggcggaatg gctggataaa gccaatgtga cgttgatgac agaaaagctc aaagggatgt
     3001 tgacgcaaag cgtgaataca catgcctatg ggaaagcgct cggcattaca gccgggggcg
     3061 tcattccggg aagttcaaag cttattcgca atgaagagac tggtattaat attttaagac
     3121 gtaatacttt gcctaaagcc tcggatattt ctaatccgtt tgagtgagtt ttggttataa
     3181 aaccatgagg aaaccattat gatcaaagcg ctgtggtttg ccattaaagt cggtgttttg
     3241 attgcgctta gtgtctggat tgctgagcgt ccggggagtg tgcgcatcga gtggatggaa
     3301 tatactttca ctatccaaat gggtttcttt ttattggttg cgttggtttt tgtgctgctg
     3361 acgttgttta tctatcaagt gcttaaaact tttgtcggtt ttccttccag tttgcggcgt
     3421 tacaacgaag ttaaggcacg ggagaaaggt tatcgtgctt taacgagggg gctgaccgct
     3481 gttgctgccg gggataagaa ggctgcggct ttgtattcta agaaggcatc gaaattgcta
     3541 gagggggata cggggttgcc tttattatta gaggcgcaag ccgcgcgtct tgatggtcgt
     3601 gaagaagatg cggcgcagag ttttgtggct ctcttggaag ataaggatgc ttcgtttttg
     3661 ggggtgcggg gattgttgca aacagctctg ggtagcggtg atgatgaggg tgctttggca
     3721 ctggcggaaa aggcgctgga tttgcatccg catcaagggt ggattttaaa gatcgtttac
     3781 gatttgcaaa ttcgtcagcg gctttggaca aaggcagaaa agatgctggc cagagttgaa
     3841 aatgctggtg ttatggcgcc ggaagtggtg cgaagcgacc ggctggcgct ttatctggcg
     3901 caggcggaaa aagatctggc cgatggttat tcttccgatg cgttgtattt aatcaaaaaa
     3961 gctcagaaat tatcgcctga ttttgcgcct gcaactattt ctttggccga atttcacatg
     4021 cgtgaagggc atccgcgcaa ggctcagaat gcggtgctaa aggcttggaa aacggcgccg
     4081 catgaggcgt tggctttgta ttggatgaca ttaaaagatg atgaaaatgt accagatgcg
     4141 ctggcgcgtt tacgccatct tgaaaggctt ttgaagatgt gcggcgaaag tgcgcgggtc
     4201 cagctgatgg ccgggcagat tgcgatagag gcggggcttt ggggggaagc gcgggcgttt
     4261 ttgcaggagg ctgagaagtt gggggcaacg acatcgctct ataaggcgtt tgcaatgctg
     4321 gaagagcgat cttcgcgtaa tgaagtggcg attaaggagc atctttcccg ggcggctggt
     4381 gctatggctg ataaggtttg ggtgtgccgg gagacaggta atatttatga gcgctggagc
     4441 ccggttgcgc agccgcatgg atcgtttaat acgattattt gggatatacc gcatgcgcat
     4501 tatggaccgg cccggttact caatgacaag gcgcatatga gcgaagcttt gattgaagcg
     4561 cctgatattg acgcggccta gagtttgatt cttccttttt tcattcctat cagcaggctg
     4621 atgccggcta aatagccacc gagatgggct tgccaggcga cgctttggcc tatcaacagg
     4681 cccggaatga tgatcaggag cgcccaaaat gcaaggattg gccaagggcc gtgtttgcta
     4741 aagcgcttag ctagtgggtt gccggtttgt gtcatagaga tgtaaattac cgcggcgaag
     4801 aggccgctga tgccgccgga tgctccgatg accgggaggg ttgagaaggg gctgagtata
     4861 aaatacagcg ctgcccctgc taaaatgcat agtgtgaaaa atatggctgt ggtgcgcgcg
     4921 ccgaagattt tttcgaaaaa catgcataga accagtccca tgacgctgtt aaaaaacaga
     4981 tgcatccaac tgccgtggat aaaggcatgg gtgacgggac ttatgaaggt ggtccatggc
     5041 catggcgcgc cgttagtgta gttggctggg acgaagccga aggtctggaa aatttcatag
     5101 cggtctgggg cgctgatcag tagatagagt gagagctgaa caatccagaa tgaggcgatc
     5161 agggcgcggg aaaaaggcgg gatattgccg gctttgaaaa aaggctgtgt atttgctttg
     5221 tgggcggctt tgcttttttg gcggtattgt ttgcgccagc gctgttcttc ttcatgttgc
     5281 tcgcggcaca ttttgtcccg ctcggctaag gaagggaatt tgatgatttt tttatcgtca
     5341 gggtctttat cgcgattatt gccattgccg tttgtgctca tggttttaat ataagggcat
     5401 gggtgaatgg aacaaagtaa aaaaccgcct ttcggagaag ggcggtttta gagtttattg
     5461 gagttggtta atttttacat gctcactgta attctgcggt tagccaagtc cggctttata
     5521 gccgatgtcg gcgtttttct cgcgatcata tttgtaggtt gcttgctcgt cgcctgttgg
     5581 tttcataaag gatggcgggg tatatttcgt aacgaacttg cctgcctgag ccaacatatc
     5641 ggttgttgag atttttgcgt gagctatgcc gtgggcgagc ttacccataa cgttgctgta
     5701 ggcttctgcg gcgatttgtg tgcccagaac aaccatttcg atgattttac cggcttcagg
     5761 gattttccct tccatcacag tgccaaaagc ttcagaggcc atcagtttca catcggaaag
     5821 ttttttcttg gcgggttttt taccgccgcc gccgcttttt ccggatttgc ctttttcccc
     5881 ttcgtcgtct ttgtcatctt tgttgtcatt gatgatttcg atatcgacgt aggtaggggt
     5941 ttggaaagat accggatagt atttgagctg tttgggttca aggtcttttc cagatagccc
     6001 ggctttgact tcaattttaa tcttgtcggg attacgccag tccagcacca cacagtggct
     6061 tttgtattcg ccgggttggc ggcaagaatg aaccagacgc ttgccgggat aaaaagcctc
     6121 ggtgatgcct gcttcttcta aaaactgttt tacatttacc tgcatgttat accactcctc
     6181 tatattgccg cagttcattg tatttttggc ttgttttgcc aattcgtttt tatcgaaatt
     6241 atgtctataa tgtacgtaat tcgttaaaaa agatttaata tatttaaaca tattgaaaaa
     6301 gggcaaagaa tctttgtttt tgcgcctttt aaagcttttc tgttatactt accggaggag
     6361 taatctggga cggtttttta tgattcgcag gttttttatg attcgcaggt tttttatttt
     6421 gagtattgtt tttctcggcg tgggattcgg tccggcattg gcgcaggagt tatttttgcg
     6481 taaagatgat ggttcggcaa aagatgggaa acccacattg tatatgaagc ccagagagag
     6541 cgggtttact gaccagacag agcattttgc tgtgaaatat aaggacaagt tgaagcttca
     6601 gcagcaaaaa ggcaacacca aggttcttat cagttcgttt gaggaaatgc aggagcttgg
     6661 gtttaaaccg cgcaatgcga aagaaataga cgcctatggg cgtatgcacc gggcgctttc
     6721 acagaatata atgtataagc ggcgtgaagc tttgattgcg catttggaga agcaagaacg
     6781 ggaggttgaa gcgatgcttg ctcgccggga tgccggagct ttgaaggttg acgatcagaa
     6841 agatagtgca acagtttcta aagagacgtc tgaggatgct cagggcgggc atgatactca
     6901 aacgacgaaa atcctaaaaa agccgagggt ttatgttaag aaaaccgccg atgagaaagc
     6961 acggccgact aaggttttta aggattatta gacctcattt tacatgagag tcttttgaat
     7021 aaagcagtct cttgatgggc tgctttttta tttcttgtgt gctaaaaatt ttgtttatgg
     7081 atgtttttgc agaatttgct gttcagaatt gggcggtgtt aattggtggt ttggttgttc
     7141 ttggtgcact ggccggtttt ttggcaggtt tacttgggat tggcggtggt attgttttag
     7201 tgccgggtct gttatttata ttttctgctt tggggcttgc ttccgagagt ttgatccatg
     7261 tttgtgtggg aacgtctttg gctcttattg ttcctaatgg tttgatgtct tcgcgggcgc
     7321 attggcaaaa aggcgctgtt gatttttctt tggttaagcc tatcggtgtg ggtgttttgg
     7381 ttggcgtggt catcggcact ttgattgcag atcgccttga gggagtgcag ttacagcagg
     7441 tattttctgt tgtaattgtt tgtctggcgg cggttatggc gataaatccg gcgcgttttc
     7501 acttttttaa aacgatgccg ccccaaccct ggccctctat tgccggtagt tttatcggag
     7561 cgatttcatc tttgataggg attggcggcg ctactctcag tgtgccgttt atgagcctgt
     7621 gtggtgtgga tatgcgccgt gcaattggtt cagcggcggc attgggcgtt gtgattgccg
     7681 tgccgggtac gttcggtttt atcctgaccg gtttgggaga accgggacgg ccgccgtttt
     7741 cgattggcta tgtgaatatg ttggcctgga ttacgatttt gccaagctct ttgttggctg
     7801 tgcgcgccgg ggtgtgggca gcgcataaat tgccgttaga ggtgatgcgt agggtttttg
     7861 ccggattttt agtgctggtg gcgttgaaga tgggactggg gttatgagcg cagtcataac
     7921 ccatagtgat acaggggtta tccaccgact tatgcgtgtt gatacccgtt attttcaggg
     7981 aggtatgttg tgtcttgcga ttttgattta tggcgcgtgg ggatctccaa cgccagattc
     8041 gccggggtgg cttgagctgc ttattgctgc gttgctggct gccagtgtag gaatttccgg
     8101 tcttatgggc gcttttatat ttagaacaaa agatccgagg tggaagcaga cggggcaaat
     8161 cttgttgatc tttggtttga gtgtgccatt gcttggtggc ttgtggggcg ggtatgaacg
     8221 tggatttata attcgggatc ttgccgcttt tggttttatg gttttgccgg tgtttttatg
     8281 gcctttaata caggtacaag cgaatatggc gcggcctttt gtttatgctg ctgcggcgtt
     8341 aggaattttg tttgctttgc gggcgagttt tgatgtgccg agtttggctg ttggggaaga
     8401 actattctac ctggcgaata tgccaagtgt tttgtttgcc gctgtgtttt tgggtggaac
     8461 ggctatgcag atttttgttc gtgattgtac tttgcgcgcc gttttggtgg cgtttgcact
     8521 gttggcgttt gtgggggtga gtttatggtc gatgattgaa atgcagcagc gcgccagtct
     8581 tggagtgttt gttcttagta tagtttgtgt ttatggctta tgtctttggc gtaatgcgcg
     8641 acggagtttt gttcttgtct gttttttggt cattttggtt gtttttcttt ggcctgaggt
     8701 cggtaacgtg cttaacgata tggagaggaa aactgcgttg gttggtggaa atatgcgcgt
     8761 cgaagaatgg cgcgcagtgt gggaggaaat ttccattcat ccgctgagtt tgttttttgg
     8821 acagggatgg ggtgcgacat tttcatctcc agcagtggcc gatattaggg tgaattatac
     8881 acatgggctg ctttctagcc tgttgtttaa aaccggtttg gtcggttttt ccctgggggg
     8941 tgtttacttg ttggctttgg cgcgtacggg tctacggtta tttgctcatg cacctgtttt
     9001 ggcgctggcg ctcgccgggc cgattttgat tgatgtgttt ttatatgcca gctttaaatc
     9061 gctggatttt ggtcttatgt tggcgttggt tgtttcttgt tgtttttacg gggatggaaa
     9121 ggtgtgcggc gttgaaactg atgcagacgt gttgtattca ggtaaggtta tcaagggata
     9181 aatatatata tttttcagga gtgatctgag catccatgcg caggccgtct attctttttt
     9241 tgaaccgagt gtacccgccg gtgcgggggg cttctggccg ggttttgcgg gatctggcgc
     9301 gtagttttgc gcgtgagggg tggcaggtta cggtgattac tacgggtccg aaagccatga
     9361 gcgagcgcga tggggctgtg cgtgttgttc gtgtgaaggg ggcggaaaag cccgggggtg
     9421 cgtttgggta tatctggatt tggatgaagt tattgtgggt agcgttgcgt ttgccgaaga
     9481 cgcacttagt cgtgacgctc actgatccgc cattcttggc gattgttggg cgaattctgc
     9541 agtttttcaa aaggagccat catattcact ggtgtcagga tttgtatccg gatgtatttt
     9601 cggcactggg ggtaaagttt cctgagttca taatgcgttt tttgaagaac cgggtacgcg
     9661 cggccatgaa gcgggctgat aaagttattg tgattggccg gtgcatggca aggcatttga
     9721 actatgatgg gtttgatccg aagcaaatta cggtgattcc caactgggta gattctgaat
     9781 tggtgcagcc tttgcaggat gatgattgcg aacctgtata tgttactact ccggaaagct
     9841 atcgcagtta tgaagagcag cgaaaacatg ggccaaagtt tcgcgttctt tatgcaggga
     9901 atattggtcg tgcgcatccg gttgagacga ttttgaaggc ggcggaaatt ttgaatgaaa
     9961 ccaatcctga tgtggagttt gtttttgtcg gggacgggcc gcgccatgag ataattaacc
    10021 gtaagcgtat acttatgcat ttggagaata ttcgtttatt gccgtatcaa cctgtgagcc
    10081 gtttgaaaga tttgatggaa agtggtgata tgcatttgat ctctatggat gggcaggctg
    10141 ggggattgct tgttccggtg aaattgtatt cggcgttggc ggcacatagg ccgtgtgtgt
    10201 ttatcgggcc ggctcattcg gaagcggcaa aagttattga ggactttaag gctggggtgg
    10261 tttgcgctcc cggcgatgta caagggcttg ccgaacatat tcgacgctat cgggaaaata
    10321 gtgataaatg gttttcagat tttgaagggg ccaaggctgc atctgaggtt tttgtgccta
    10381 aagaatccat taatgcctgg attgaaaggg cctgggctgt ggttgatcag gatgttcatg
    10441 tatgagtcaa gacaggtacg atagtgaagt gacaccaagc aaccgtgaac aaacactggg
    10501 tgagtttatg cgtgaagttt ggtccgggcg tgtttttgtt ttcggcgggc ttatgattgg
    10561 tttagtgctc gcctttgtga tgatgagcag tgctgttcca cattttcggg cagaaatgat
    10621 tcttgctccg gccagtccga tgaatatggc ggcttcgccg gtcaactatg ccgggggcgc
    10681 attagagcaa aatagtgagg ttatgagttt tacgcgtttt gaggtgagct ttaaaggtgt
    10741 gccgcctgca gcgctgttgt tgcgtgatcc ggaaattacc gatgggctgg cgcgtgataa
    10801 ggctttttct ttttcaaggc ctgagcaggg ctggagcgct gaaaaactgg cggaatatat
    10861 tgcgcgccgg gttgaggtcg atcctgtggg agaaacgtct ttgcgggctt tacggtattt
    10921 gcatccggat aaagagtttg cggcgctgtt tttacagcgg ctgcataata ttaccgatgg
    10981 tcttattcgt catggtattc gccgcgaggt gaatgagcgc attacatatt tgaacaaagc
    11041 gattgctgaa aacatgaatc cggagcatcg acgcgctttg actgcgttgt tgatggaaca
    11101 agagcgcttg aagatgctgg tgtcgattga tcagccttat gcggcgagta tcgtggtgcc
    11161 tgcttcggtt tctgtgaagg cgcgttggcc ggacgggtcg ttgatttatt cgggttttgc
    11221 atttgttggc gctttgcttg gttttgtggt gtttagtgcg cgcggggttt gccgtgagga
    11281 tatatttgct gtgtttccgg gagggaaggt taaacagcag gaatggtttt tccctgagag
    11341 cgggaataat aatgagaaac catcttttgc ggccgggcga cggccattaa caggaaagaa
    11401 acgagatcag gatcctgatg aggattctga tcttccctcc gaggcggcgg agtaggaggc
    11461 gtgtatggga aaccctcctg ttagcgttat tattactacc aaaaatgaag agcgccgtat
    11521 tggcgcatgt ttagaggtgc tttcgcgttt tgatgatgtc tgggttgtgg attctgatag
    11581 cagcgatcaa acgcagcaga ttgcgcgcaa ggccggagcg aggattaaga attatgtctg
    11641 ggacggatgt tatccaaaaa agcgccaatg gtgtctggag aatttggcgc ttaaatatga
    11701 gtgggtgttt ttcgtagacg cggatgaaat tgtgccgcgc gggctggttg atgaaattgc
    11761 cgggcttgat tggtgcgcgg ccggatattt tgtgaaggga ctttatgagt ttgaggggag
    11821 gtttcttcac cacgggcttt gtaataacaa gctggccctg ctggatcggc gcaaggttga
    11881 atttccggtt gttgacgatc tggatattga gggaatgggt gagatagagg ggcattatca
    11941 acctgtgctt aagaatgggt ttgagaatga agatattggg cagaccgaaa cgcctttgtt
    12001 gcattatgcc tatgatgatt tgcaaagttg ggaggaacgt caccatcgtt atgcgcgctg
    12061 ggaggcgggg atgaatgcga agggtgcgtg gcctgtggat ccgcgcccgg tgcgtcaatt
    12121 cgccaaggaa gcgtttcgca acgtcccgtt tcgcggcgta gttgcgtttg tacattgtta
    12181 tatttttaag cttggttttc tggatggggc ggcggggtat cgtttcgcgc ggtcgcgggc
    12241 gcgttattat cggatgattt cagatgccag taaagcgatg gtacaaccct gcgcagagtc
    12301 caaagagcag cctgggcgct tgtaattatt ctattttgga cggggccagt gagctgtaaa
    12361 tcttttcgga tttgatattg ttcgcgtcgg cccaatactg cgttcgtctg cgaaacgccg
    12421 ccgctttcga aaatgcagat ggggaagggg caatacaata tattgccgtg gtcttttaaa
    12481 aaccgtgcac tgagatcgta atctgcggcg attttataac gcaggtcata gcgcatatcg
    12541 ccgagggctt tgcggttata gagcatggct tgatgatggg tgaacatacc gctgcgtaaa
    12601 tcagctgtgc gtgcgggctt gtaagcgccg tcttcaagag aatctccata aatgaagtcg
    12661 gggtgattgt gagctgcctt tttgatgttt tggagcgttt cgggcgcggc gagggcgtct
    12721 ccggcgttta ggaagagtaa atattttccg ctggcgcgct ctaccccttt attcatggcg
    12781 tcatagatgc cgcagtccgg cctgctggcc cattttgcgt cggtggtttt tagatattgc
    12841 ggggtttcat ctgttgaagc gccgtcaatc acaatccatt cgaaatcatc gcagttttgg
    12901 tcctggagag atttatgcgt ggtctttagc cctgcgatgt tgttgagcgt gacggttata
    12961 atggaaaaaa atgctgcttt ttcaggggct ttgctcatct tattttgaat tttaacagaa
    13021 tattaaggat ttggaagcat tataggggtg cggaaaaggc cttaaaaatg ggcagtgaat
    13081 ttaagtgcta aacgcaataa tatgagaggg tgtaattgaa tggccgaaag ggcgatagat
    13141 actggtatag ccaaggctct ggcacagcaa ttcctcaaga gattggtgga tgattttaag
    13201 ttcggctccg atgtggatac gatggagcag gttgtgaacg gtttgttacc gcgcgatgat
    13261 aaagggaagc ctaaatttga cgatgcgtcg cgtgcgcagg ccgccaagac gctgattacc
    13321 ggattaaacg cgctttataa gaaaagtgat gtcgatagcc gtgttcaggg ggctattgag
    13381 aacatcttta aagatgcccg tttaaaaacc gcgcatgatc tggccacgca gttgaaggac
    13441 cagatgcgaa cgcaggaatt tttggcgctg ttgaagaatg acgatgcgga tttcggtttt
    13501 tattttaatg acaggcagat caaagctatt aagcacaatt tgaaccttat atcgccgggg
    13561 caaaatggtg ggccggggtt aggcgatatg atagctaact ttttgggttt gcgcggtgat
    13621 cttgatggta cggcagagcc aacggcgcgt agcgaactga ccgaaggatt tcacgctatg
    13681 accaatggcg ggcaaaacag cctggccaag ttttgggata aagaccgcgc tggggataat
    13741 aaggcgttta atgtctggaa cccaccgagc ggggaagaac aaaagaaagc gtttttctcc
    13801 aaaaccggga tgatgaattc caaaggaaag agtgaatatc agcattatga cagcgatgcc
    13861 aaggttttta aaatcaactt tgaagacatg attctggcca agtgggaaaa agacggcatt
    13921 gttaaattca atgggcctta tgcgcgtgag cattttatta atttcgttca aaatcaacgt
    13981 atgagcggcg agttgggcgc gcctctttcc gctgcgctgg aaagcaaggg gcagatttct
    14041 tttgcttctg gatctgcgcg ggctgatttt gatgagttta cgtccggttt ggccgggcaa
    14101 ggtctggaca attatgaaat tgcggaagca attaaaaagt acgcagaaga ttctactaat
    14161 ggtgtgacgc ttcctaaagc ggggctgcat aaatttgaga atgagttgcc ggaaacgatc
    14221 tcgctgtttc aggatgcaaa aagtccggac gagtttgcgg ataaggtttt ggcttttgcg
    14281 aactggtatc cgggaagcgg gattgtggtg gtcgatccca aagttgagca ggaaacgcca
    14341 agcgtgatga ggggcaagcc taatgatgcg ccggcgcaca gtaaggcttc tgcttcgaaa
    14401 acgcctgagg aaaagctggg cgagattaaa gtggtagctg gccagatcac aacgccatct
    14461 gcaagcccgg aacctccggc ggctgtgcct gtaatatctt ctcccggcgt tcagcccagt
    14521 taatttgtta tttaattgct gtcggcaaca tcatcaataa agatggcggt cacagctaga
    14581 ttcgaaagga tttggctgac atcttttgcg ctttggatga agtcaaaggg tttggggtcg
    14641 cgcggtacga caatggtgga gccgggcggg atcatcaccg ggttatagtt ccatgaagaa
    14701 acctgtagcg gctgggctgc gccgtcagga taaatgacga aggtgcggtc tttgtcggcg
    14761 tggaaggtga agccgcccgc ttcatggatg taatcgagcg aggctttgcg ctcgcggaat
    14821 tg
//
LOCUS       DAOSWR010000044        14728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2579_length_14728_cov_5.119335, whole genome shotgun sequence.
ACCESSION   DAOSWR010000044 DAOSWR010000000
VERSION     DAOSWR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_14728_cov_5.119335"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..425)
                     /locus_tag="PK513_04785"
     CDS             complement(30..425)
                     /locus_tag="PK513_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81796.1"
                     /translation="MSSALSDLKILLVEDQAEARAMIRNMMGELGITQIFEASDGREA
                     LTFLDSAYDFIDLLVCDWNMPGMTGVDLLRQLRSVDSTMPFLMVTGRIDMDSVVEAKS
                     AGVTAYIRKPFSSKQLEAKLRIILHKIAA"
     gene            complement(425..2560)
                     /locus_tag="PK513_04790"
     CDS             complement(425..2560)
                     /locus_tag="PK513_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO81797.1"
                     /translation="MFTESYYWLLASGIFLLAVLMLCGFMFYMMRCRCKTRNYTSALE
                     KAADFIGNGLVFFGVDDRLVLANVQAHEFLPDLLENKGKRLSGEPLNLQDFLIYFYDM
                     AVECDESLINTLGRSAMTNTDIGFREVIMTAGNRLCLVEVQKIPHVGTNLILIDVGDL
                     KNQEDYLLKLNHYNHELTQAIQAATSGIVVTKQDHVSGLQRIVFANKAFCEDMHLSRD
                     DILGKNVHEVFATIVDAKTMEKIYQISEGHEDGSVELSILCSGGEKCCGACWYELKHT
                     SVQGYTGKLDLSIWLLNDMTALKMREAELSKTQKLEALGQLAAGVAHDFNNILSIIDG
                     YARIAGKNWDDKARAMDDLDRIRTASRRGANLIKKMLTFSRHEIVDDTVIDLGAVVRE
                     QELLLRPLLDASVKCSVLIDHQEMYVECPIENITQIIMNLVLNARDVMPDGGVLLLEV
                     RACPQKTLPAVLLKQNSDKSYASIIVSDTGMGMPKDVVDRIFDPFFTTKEQGKGTGLG
                     LSMVYGLVKQIGGHIDVFSTLGQGTTMSVYLPLSDKVPKKILGGEDNLSSIRFDGYSV
                     LVAEDEPDLLALVSNMLEDLGMKVLRASNGNEALAVQDDYEGKIDLLLTDVVMPELNG
                     VDLADLLKELRPDVNVIFMSGYPAKGQMARVEIPEDAVFIAKPIEYDALVKVIYTILS
                     SGVGESIEDSVQISRWASKGGIEKEEERF"
     gene            2733..4847
                     /locus_tag="PK513_04795"
     CDS             2733..4847
                     /locus_tag="PK513_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOO81798.1"
                     /translation="MPCVKPDFFYKVLLHSAALWGGAIYAHAAPPQPSTSLYGPLGLL
                     AIPSARMDHAGTLRAGVSTLDPYVHSWLSVQLADPLSITLRQSAEISNINEDAKRLYP
                     GVDFKLRLLEESAYRPEIAIGARSAFGHKRMAGEYISASKRYKDFDFTAGLGWGRLGS
                     AAHFKNPLKSLSSHFGGNRNLDGEMPSGPENWFTGGDIGLFGGVEYFTPLKGLSLKAD
                     YGADRFKAEQAAFDYNAPAPWAVGLNYQPAPWIDLSLGMQGTDKLMARLSLQGNVKKW
                     PDQDRKYTANDESPFRPYRTGLALPAEMELKAAGENIMLYDAQTDYETASATLTLENN
                     RSSPCQLARAFKHMANHAGPSIERLEITPIHLGLRGPKIALLRADLENALGRQAGSAE
                     EIWHNMEIIPSAENPLRKHHQPLEYGYGLRDYALTLDTQASLSEEDSGTLYRTALVAG
                     AQAPELFGLIDNFVSFRLNLADNLDRLTDIRPRAALPVRSDVDRFARRTIALDTAFNA
                     FTHSFRSDLHTSLMGGYLEEMYAGAGGEILYRPYKARWAVGAESFLVLKRNPESTLNL
                     GLNGDHLLSGHIQGWYDLPHWDITVNAKAGRYLAEDLGATLSLQKRFHGGAVLEAYST
                     ITNQSDFDLFGGTTHADHGIRLALPLGGIKYIPRNAKAKFTLAPFGRDIGQKLENPLP
                     LYELTEGFSARHLAQYWDEITP"
     gene            complement(5017..8403)
                     /locus_tag="PK513_04800"
     CDS             complement(5017..8403)
                     /locus_tag="PK513_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HOO81799.1"
                     /translation="MNTFLLSFDANTSFSGGNPPTLEILVGGVVVSSVTMVSGATSYD
                     VFIEYTGTAPSSLSFRFDGASGDPGDTITFTAVSINSSALNLGTDLTATILAQAQSSV
                     VSAGTDLFGHTTPSIGVPNVTGTAGDDANLSGGNAADTIDGLAGADRIRGLGGDDTIN
                     GGDGDDMIFGEGGADTVIAGNGNDMVFGNDGDDILYGEADNDYLIGGAGNDILNGGAG
                     NDGLLGDAGDDILFGEDGDDWLIGDDGDDILFGDDGNDTLIGGADNDALAGGDGDDYI
                     SGGTGNDLLSGGDGADEIIAGTGDDIASGGAGNDSIYGDDGADELAGGDDDDYISGGD
                     GDDTLDGDAGVDVLVGGAGADTMNGGTGDDILHAHGLDAVAISDILFNNPNVVYSQET
                     GSFYQFVSANVDYAAAVAAASATTLNGVAGHLATITSAAENTYVSGLLSDDSYISGAD
                     IAQNGTWQWNAGLEAGVQFSDVSGTSVNGMYENWASGQPQVNTEYNAILYTTGVWHDW
                     VDTSTHSYVIEWEAGLMSDDGAADILSGGAGNDWIYGYDGDDTLNGDGDDDVIFGGDG
                     SDIIDGGSGNDVLFAYDATVQTSGTAGGGSTVTALEATFDTDEDGFTYADGFPGVGGS
                     DGANVTISGNYISGDGNTSAGSIEVNINSTGNPTGPLSGSYSQTITTTADLTSVQMSI
                     SYSMFLDGTTETGENLYLYVTVDGTQYGLGGNNWVDELNGVNGDGADHTTGWLTETLN
                     IADLSAGSHTIRIGAYLNDTSQSSEDGWIRFDDILLTGVDTTVGSGSTGVEDAGETNV
                     VSGGDGADTIYGSTGMDTLNGDAGADTIYSASANEAWDALVAQVLADNAGVVYSVETN
                     SFYQHITTNATWTTANTNANAATLTGLTGNGYLATITSQAEQDYVWSLGGGNNLWGGA
                     TDVTTEGNWVWQGGSESGLTFWTGGAANSGGAAINGHYDNWRSDGSEPWGGSSTADYF
                     ILNSALGGQWWTENNNAVYTGNAGADYVIEWDADELLTSVDYTTINGGAGADTLYGND
                     GIDIFLFETATWDATDTIENFSATGRDAIDISDLLSGYSYTTSNINDFVTLTEASGNT
                     TIAVDANGATGGASFTNVAVLNGVTGLDLYQMIAADNLIV"
     gene            complement(8924..9916)
                     /locus_tag="PK513_04805"
     CDS             complement(8924..9916)
                     /locus_tag="PK513_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010300037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO81800.1"
                     /translation="MDECSDKEKDTLRFIISEEQVGQRLDKALAALADGFSRSRLQGI
                     IQDGALHLNGLVCRDVSHKVSLGSVIEIEIPPVEEAAPQPEKIALNVIYEDEDVLVIN
                     KPAGLVVHPGAGNASGTLVNALLYHCGESLSGIGGVARPGIVHRLDKDTSGLMVVAKH
                     DRAHQGLAAQLEDRSLSRIYEALALKVPVPVKGVVDMPIGRDPRNRLKMSVKGQNAKP
                     ARTHYRVLKAYGEALSLVECKLESGRTHQIRVHMQALKHPLIGDPLYRAQDTAVRGAM
                     KKAGYEEDVIMAALNFPRQALHAKSISFVHPLSGEVHNYEVDAPDDFFNLLKKL"
     gene            complement(9918..11258)
                     /locus_tag="PK513_04810"
     CDS             complement(9918..11258)
                     /locus_tag="PK513_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81801.1"
                     /translation="MINRLVLFSAFVGVFALWSGGVWAQNCDIQYEAIIKLRAPTLGS
                     YNVWDSVHGEIPTHERFKSALVNETGTVLAAGERVLPAKGDRKDLLLVEIGRNGRVLW
                     EKDYEVPGLAELVKVLPHDKGMVMLANITPPKGRQHIWLGVFNLQGDLLFSKVVRDPG
                     NHLRGHDMEPSASGQSFVIAASASSPKEDKPDWSVLYRVNSAGVVIADHAFVIGSENA
                     VYDLYPMKDGGYVASGSIDNAIGRKTGWVMRIAEDLNMMWQKSYPRGAAAEIVRAHEM
                     LADTLAVVGSALPMGDGNRAAWVMVVNDATGDVVWQRYLNGSLHFDGRDVMVSEDGLI
                     SVLIDGQTPEGSEEIEHVRLLTLNPRGVLFSSDEFFNGEAVDAYALMASKGAERLIIG
                     ETRVAYKIEHSRAINEQGPEIPDEVVRSQEGWVVAATGVDPYPDPCRQRERVMP"
     gene            11297..12412
                     /locus_tag="PK513_04815"
     CDS             11297..12412
                     /locus_tag="PK513_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOO81802.1"
                     /translation="MTATNVYASNKRPLQESTTNPMKLLIISDAWHPQINGVVRTYEH
                     ISQEMQKLGHTVKVIGPADFPMSVPMPGYKEIRLAIAPYRKLQRIIKKYNPDHIHLPT
                     EGPLGWAGRKYCLRNERRFSTAFHTRFPEYVAQRAAQILPSSHDFVHARVVNYVRRFH
                     APSSGMTVATGSLEETLKNWGFQNPMHRVTRSACLDTFTPGEKTLFHDLKGPIALYVG
                     RIAIEKNIEDFLKMDWPGSKVIVGTGPQKAELEQKYPDALFVGKKTGAELVEHYRSAD
                     LFVFPSRTDTFGIVLIEALACGLPVAAYNVTGPKDIITKDFLGSLHDTNLSLAAKTAL
                     QNKDLEKRTTHVHKEYTWQHAAAQYEDMILNKILQTR"
     gene            12424..12789
                     /locus_tag="PK513_04820"
     CDS             12424..12789
                     /locus_tag="PK513_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81803.1"
                     /translation="MAHILIAEDDQSMCSFLQLALEKAGHNVQIAYNGIDALKIIEES
                     NPPIDLLLSDIVMPGMDGVELSNKATKTHPSIKIMFITGFAAVAMEREDLDREKTKVL
                     SKPFHLNDLVEQVETLLST"
     gene            12937..14718
                     /locus_tag="PK513_04825"
     CDS             12937..14718
                     /locus_tag="PK513_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81804.1"
                     /translation="MAENTHIAEEYIKKLGGNNTVWADTIAPPTAEQTARLANFANEG
                     MKNSLLQGYAADMGPDTNFWNKIGTKDGFHTAPVDGKTGQFRFENLTQTTLPTLGGTV
                     GAVFAPLEEEWLVGLDFEVNVKGTILVEMAKPGEELGPAGYKLSHNVKTDLNFYNEKV
                     EDPLKVSGKAFWIEKSEEIPSFAFKHQEETNLGNAMADLGNAPAGLSFASLDTAKNPD
                     MSGVISDTTPLPPAAPVEKSVDPFTQLNPEEAHKGYSGPAPALIDAKVTFSGEYQINA
                     KINVEVEHTLTVSRQDYERIKAELDGQTIYSASVAGSVTVWNPGADSEGNASEFFVHV
                     GLDKLEMKGGINETVAMTKLLQDEKDGAGQQYIAELNGRAPPPVNPYLQDSPGKTMIA
                     KASPTGTADDVRLAQASCNLLSDANLAIDGDPGTKTQSCVGTVLGDDYVADDWKQNAE
                     NLQEIVALSPEAAEKGRILATKTLNGEIKDFSKEAYETQAFLKAKEGIAFDGDIGRGT
                     RAAATAHLDMENQPSIEAIANAPSPGKGTPSLSCEFGKVCGPENAANDPSFDQPETDA
                     RAVAKTQPPIQPTREVVSVLRPALRRS"
BASE COUNT         4179 a         4481 c         2847 g         3221 t
ORIGIN      
        1 tttctggatt taagctgtga gtccggaccc taagccgcta ttttatgaag aataatccgc
       61 aatttcgctt caagctgttt agaagaaaaa ggcttacgaa tatacgccgt cacaccggca
      121 gactttgctt caactacaga atccatatct atgcgccctg ttaccatcaa aaacggcata
      181 gtagaatcaa cactgcgcaa ttgtcgaagc aaatcaaccc ccgtcattcc tggcatattc
      241 cagtcacaca ccaaaagatc aatgaagtca taggccgaat ctaaaaatgt cagcgcctcg
      301 cgcccgtcac tagcctcaaa aatttgcgtg atgccaagct cacccatcat attacgaatc
      361 atggcccgcg cttcagcctg gtcctcaacc aaaagaattt taagatcact taaagcagaa
      421 ctcatcaaaa tctctcctct tctttttcaa taccaccctt actggcccac cgtgagattt
      481 gcacggaatc ctcgatactt tctccaactc cagacgataa aatggtataa ataactttaa
      541 ccaaagcatc gtattcaatg ggcttggcaa taaacacagc gtcttcagga atttctacgc
      601 gggccatttg cccttttgcc ggataaccac tcatgaagat aacattgaca tccgggcgta
      661 attctttcaa caaatctgcc agatccacac cattgagttc aggcatcaca acatctgtca
      721 gcaatagatc aatttttccc tcataatcat cttgcacagc caaagcctca ttgccattgc
      781 tggcccgcaa caccttcata cccaaatctt caagcatatt actgaccaat gccaacaaat
      841 caggttcatc ctctgctaca agcacgctat aaccatcaaa ccggatagaa gaaagattat
      901 cctctccacc caaaattttc ttgggaacct tgtcactcaa aggcaaataa acgctcattg
      961 tcgttccctg ccccaaagta gaaaacacat caatatgccc gccaatttgc ttcacaagcc
     1021 catagaccat agacaacccc aacccggtgc ctttcccttg ctcctttgta gtaaagaaag
     1081 gatcaaaaat acgatcaact acatccttcg gcatccccat gcccgtatct gaaactatga
     1141 tggacgcata agatttgtca gagttttgct tcaaaagaac cgctggcaaa gttttttgcg
     1201 gacaagcccg cacctccagc agcaacaccc ccccatcagg catgacatca cgcgcattca
     1261 agaccaaatt catgataatt tgcgttatat tctctattgg acactcaaca tacatctcct
     1321 gatggtctat caaaacgctg cacttaacag atgcatccag caaaggccgc aacaacaact
     1381 cctgctcgcg caccactgcg cctaaatcaa tcacagtatc atcgacaatc tcatgacgac
     1441 tgaatgtcag catttttttg ataagatttg ccccgcgcct tgaagctgtg cgaatacgat
     1501 ccagatcatc cattgcccgc gctttatcat cccagttttt tccggcaatg cgggcatacc
     1561 cgtcaataat cgacaatata ttattgaaat catgcgccac tccagccgcc aactgcccga
     1621 gagcttccag cttttgtgtc ttggaaagct cagcctcccg cattttcaaa gccgtcatat
     1681 cattcaacag ccaaatcgac agatccagtt ttccggtata accctgcaca gacgtatgtt
     1741 taagctcata ccaacacgcc ccgcaacact tctccccgcc agaacataaa atgctcaact
     1801 caacgctgcc atcctcatgc ccctcactga tttgataaat tttttccatg gtcttggcgt
     1861 cgacaatagt ggcaaaaacc tcatgaacat tcttaccgag aatatcgtcg cgcgagagat
     1921 gcatatcctc acaaaacgcc ttattcgcaa atacaatcct ttgtaaacct gaaacatggt
     1981 cttgcttggt cacgacaata ccgctcgtcg cagcctgaat agcctgggta agttcatgat
     2041 tataatggtt aagcttaagg agatagtctt cctgattttt aagatccccg acatcaatca
     2101 aaatcaggtt cgtaccaaca tgcggaatct tttgcacttc aaccagacac aaccgattcc
     2161 cggcagtcat aatgacctca cgaaaaccta tatcagtgtt agtcatcgct gaacgtccca
     2221 gcgtattaat caaggactca tcacactcaa cagccatatc ataaaaataa attaaaaaat
     2281 cctgaagatt caaaggctca cctgacagcc gcttcccctt attctccaat aaatctggca
     2341 aaaattcatg cgcctgcaca ttggcaagaa ccaaacgatc atcaactcca aaaaaaacaa
     2401 gcccatttcc aataaaatca gccgcctttt caagcgccga tgtataattg cgcgtcttac
     2461 aacgacaccg catcatataa aacataaaac cgcacagcat caaaactgcg agcaaaaata
     2521 taccgctagc caacagccaa taataagatt ctgtaaacaa aaactccccc ttaagcaaaa
     2581 ctactgattt gcaactctaa gtttatgttt ataactaccc cttgtctaga catctctgta
     2641 gagaatctga atacccgcgt atatactaca acaaatctgc atattctctt caaagccttg
     2701 cactaaacgc ccattacaaa acgcttacaa caatgccttg tgtaaaacct gacttttttt
     2761 ataaggtact cctgcacagc gccgcactat ggggaggcgc aatttacgcc catgcagccc
     2821 cgccgcaacc ctccacctct ctctacggcc cgctcggcct cctcgcaatc cccagcgccc
     2881 gcatggacca cgcgggcaca ttgcgcgcag gcgtttcaac actcgatcca tacgtgcata
     2941 gctggctcag tgtccaactc gccgatccac tctccatcac gctgcgccaa agcgccgaaa
     3001 tctccaacat caatgaagat gctaaacgcc tctatcccgg cgttgatttt aaactccgcc
     3061 tgctggaaga aagcgcctac cgccccgaaa tcgccatcgg cgcaaggagc gcctttggcc
     3121 ataaacgcat ggccggagaa tatatctcag cctccaaacg ctataaggat ttcgacttta
     3181 ccgccggtct cggctggggc cgcctcggaa gcgcagcaca ctttaaaaac ccgctcaaat
     3241 cactcagttc tcacttcggc ggtaatcgca atctcgatgg tgaaatgcca agcggcccgg
     3301 aaaactggtt taccgggggt gacatcggcc tgtttggcgg cgtcgaatat ttcactccgc
     3361 tcaaaggcct ctctctaaaa gctgattacg gcgccgaccg gttcaaagcc gaacaagcgg
     3421 ccttcgacta caacgcaccc gctccttggg ccgtaggcct gaactaccaa cccgccccct
     3481 ggattgatct cagccttggt atgcaaggca cagataaact catggcccgc ctgtccctgc
     3541 aaggcaatgt caaaaaatgg cccgatcaag accgcaaata tacggctaac gatgaatccc
     3601 ccttccgccc ttaccgcact gggctggccc tccccgcaga aatggagctt aaagccgcag
     3661 gcgaaaacat catgctctat gatgcacaaa ccgactacga aaccgccagc gccaccctga
     3721 cattggaaaa taaccgctca agcccatgcc agctcgcccg cgcttttaag cacatggcca
     3781 atcacgcagg cccctccatc gagcgcctcg aaatcacccc tatccacctc ggtttacgcg
     3841 gcccaaaaat agccctgcta cgcgctgacc tcgaaaacgc actgggccgc caagcaggca
     3901 gcgccgagga aatctggcac aatatggaga tcataccctc cgccgaaaac cccttgcgca
     3961 agcaccacca accactggaa tacggctatg gcctgcgcga ttacgccctc accctcgata
     4021 cacaagccag cctgtccgaa gaagatagcg gcacccttta ccgcaccgcg ctcgtcgctg
     4081 gcgcgcaggc gcccgaactc ttcggcctga tcgacaattt cgtgagcttc cgcctcaacc
     4141 ttgctgataa tctcgatcgt ctcaccgaca tccgcccgcg cgccgccctg cccgtacgct
     4201 cagatgttga tcgctttgcc cgccgcacca tagcgctcga cactgctttc aacgccttca
     4261 cccacagctt ccgctccgac ctgcatacga gcctcatggg cggctatctc gaagaaatgt
     4321 atgccggcgc cggcggagaa atcctctacc gcccctataa agcccgctgg gccgtcggcg
     4381 ccgaaagctt tctggtcctt aaacgcaatc cagaaagtac gctcaatctc ggcctaaacg
     4441 gcgaccacct gctcagcggc cacatccagg gctggtacga tctgccccat tgggatatca
     4501 ccgtgaacgc caaagccggg cgctatctgg ccgaagatct cggcgcgacc ctgtccttgc
     4561 aaaagcgctt ccatggcggc gcagttttag aagcctattc cacaatcacc aaccagagcg
     4621 acttcgacct gtttggcggc actacccacg ccgatcacgg catccgtctc gccctgcccc
     4681 tcggcggcat caaatatatc ccgcgcaatg ccaaagccaa atttactctc gctccgtttg
     4741 gccgtgacat cggccagaaa ctcgaaaacc cgctgccact ctacgaactg acagaaggct
     4801 tttcagcccg gcatcttgca caatactggg atgaaatcac accgtaaatt ttggaaaaaa
     4861 taaacacctc cgccccaagg ggcgaggtat cacgtcattg caaaccgcca aaggcggtga
     4921 agcaatccag aatttatcct tgtggcactg gatcgcttcg cttcgctcgc gatgacgatt
     4981 acgccgcaag cagcgaggaa ttaaacctga aagagattaa acgattagat tatccgccgc
     5041 gatcatctga tacagatcaa gaccagtgac accattcaaa accgctacat ttgtaaagct
     5101 cgcgccgccg gttgcaccgt ttgcatcaac tgcgatggtg gtgttaccag aagcttcagt
     5161 aagtgtcaca aaatcattaa tattgcttgt cgtataacta tacccgctca gcaaatcgct
     5221 aatatcaatc gcatcacgac ccgttgcgct gaaattctca atcgtatccg ttgcatccca
     5281 tgtcgccgtc tcgaacaaga aaatatcaat cccgtcattg ccataaagcg tatcggcacc
     5341 agccccgcca ttgatcgttg tgtaatcaac actggtaagc aattcatcag catcccattc
     5401 gataacatag tctgcgcccg cattgcccgt ataaacggca ttattatttt cagtccacca
     5461 ctgcccgcca agagccgaat tcaggataaa ataatcggct gtagaagaac caccccacgg
     5521 ctcagacccg tcagatctcc agttatcata atgcccgttt atcgccgcac cgccggaatt
     5581 tgcggcaccg ccagtccaga aagttaaacc tgactcagaa ccaccctgcc agacccagtt
     5641 cccttctgtc gtaacatctg ttgcaccgcc ccatagattg tttccacctc ccaaggacca
     5701 gacataatcc tgttcagcct gagatgtaat cgtggccaaa taaccgtttc ctgtcaaacc
     5761 ggtcagtgtg gctgcattag cgtttgtatt ggctgtagtc cacgttgcat tggtcgtaat
     5821 atgttggtag aaactattgg tctcgacact gtaaacaaca ccggcattat ccgcaagaac
     5881 ctgcgcgacc aaagcgtccc acgcttcatt cgccgaagcc gaataaattg tatccgctcc
     5941 cgcatcgccg ttaagtgtat ccatcccggt actaccgtaa atcgtatcgg cgccatcacc
     6001 accgctgaca acattggttt ccccggcatc ttctactccc gtcgaaccgc tcccgacagt
     6061 agtatcaacc ccggtcagga ggatgtcgtc aaaacgaatc catccatctt cagatgattg
     6121 acttgtatca ttgaggtaag ccccaattct aatcgtatga ctccctgcag aaagatcggc
     6181 aatattcaaa gtttcagtca accaacccgt cgtatgatcg gcaccatcgc cattgacccc
     6241 atttaattca tcaacccagt tattaccccc aagaccatat tgggtcccat caactgtgac
     6301 atacaaataa agattctcac ctgtctccgt agtcccatca agaaacatgc tgtaggaaat
     6361 tgacatttgg acagacgtca aatcagccgt cgtagtaatc gtctggctat aagaaccgga
     6421 aagcggcccg gtagggttac cggtcgaatt gatattcacc tcgatacttc cggcagacgt
     6481 gttcccgtcc ccactgatat aatttccact aatcgtaaca ttggcaccgt cagacccgcc
     6541 aaccccggga aagccgtctg cataggtaaa gccatcttca tccgtatcaa atgtcgcctc
     6601 aagagccgtg accgtcgaac cgccgcccgc agtgccacta gtttgaaccg tcgcatcata
     6661 ggcaaaaagc acatcattgc cggaaccgcc gtcaataata tcgctaccgt caccgccaaa
     6721 aatcacatca tcgtcaccat cgccattaag ggtatcatcg ccatcatagc cgtaaatcca
     6781 atcattacca gcaccgccac tcaaaatatc agccgcgcca tcatcgctca tcaagccggc
     6841 ttcccattca atcacatagc tgtgagttga cgtatcaacc caatcatgcc aaacgcctgt
     6901 cgtatataaa atagcgttat actctgtgtt tacctgcggc tgcccagacg cccaattctc
     6961 gtacataccg ttaacagatg ttccgctgac atcgctgaat tgtacacctg cttctaagcc
     7021 ggcattccat tgccaggtac cgttttgagc aatatctgca ccactgatat acgaatcatc
     7081 gcttaacagc ccagacacat aagtattttc cgccgcagag gtaattgttg caaggtgccc
     7141 ggcaacacca ttgagtgtcg tcgcagatgc ggcggcaaca gcagccgcat aatcaacatt
     7201 cgcgctaaca aattggtaga aactgcctgt ttcctgagaa taaacaacat tggggttatt
     7261 aaacaaaata tcggaaatag cgaccgcatc cagaccatgc gcatgcaaaa tatcatctcc
     7321 ggtgccacca ttcatcgtat cggcaccagc tccgccaaca agcacatcaa cgccagcatc
     7381 accgtctaac gtatcatctc catccccgcc ggaaatataa tcatcatcat cgccgccggc
     7441 aagctcgtcg gcgccgtcat cgccgtaaat actatcgttg cctgccccgc cagaagctat
     7501 atcatcgcct gtacccgcaa taatctcatc tgcgccatcg ccaccggaaa gcagatcatt
     7561 accagtacca ccggaaatgt aatcgtctcc atcaccaccg gctaaagcat cgttatctgc
     7621 cccgccgatg agcgtatcat tgccatcatc gccaaaaaga atatcgtctc cgtcatcgcc
     7681 aatcagccag tcatcgccat cttcgccaaa aagaatatcg tccccggcat caccaagcaa
     7741 accatcgtta cccgcaccac cattaagaat atcgttaccc gcaccgccaa tcaggtaatc
     7801 gttatcagcc tctccataaa gaatatcgtc accatcattg ccaaagacca tatcattgcc
     7861 gttaccggca ataacagtat cggcgccgcc ttcaccaaag atcatgtcat caccgtcacc
     7921 gccattaatg gtgtcatcac caccaagacc acgaatgcga tctgccccgg ccaacccatc
     7981 aatagtatcc gccgcattcc ccccggaaag attggcatca tccccggctg ttccagtaac
     8041 attcggaaca ccgatgctcg gcgtcgtatg accaaaaaga tccgtacctg cagaaaccac
     8101 agagctctga gcctgcgcca aaatcgtagc cgtaagatca gtccccaaat taagagcact
     8161 actgttaatc gaaacggctg tgaaagtgat ggtatcgccc ggatcaccag aagcgccatc
     8221 aaatcgaaaa cttaaggatg aaggagccgt accggtatat tcaatgaaaa catcatagct
     8281 ggtggcaccg gaaaccatcg taacagagct gacaacaacg ccacccacca gtatctccaa
     8341 agtcggagga tttccacctg aaaagctggt attcgcatca aatgataata aaaacgtatt
     8401 catcgcccgg ttttctgccc ttaactagta acccaagttc tctcagtagt taaagcaatg
     8461 ccttttaacg cattgttttt tatatttatt cttattaata ataccagaat atggttagaa
     8521 aaacattaac gcataaagct taacaaaatc ttgcttaatc tgctagaaca gacaaaaaaa
     8581 tccgccccaa ggggcggttt tagaggtacc agatcggatg aagtagtctt aaaaagtatg
     8641 tctcattaaa tgtagtagtc ttaaattgta gcagtcttaa aaggagattc aaaaaccctg
     8701 ctaatattgc tttaatttcc tccgccattc gtccgcttta tttatttact cacttgataa
     8761 aatcggatct cttatgtctc aagcgggggg cctattttgc cctgcccccc ttgctcagat
     8821 ttggtcttac gcactgacct tatatcttca gaatacataa aattttatgt aaatacaata
     8881 aaattatcaa aattttatgt aaaattttat gtaagtgtat tttttacagt tttttcaaca
     8941 ggttaaaaaa atcatctgga gcatcaactt cataattatg cacctcacca ctgagcgggt
     9001 gcacaaatga aatacttttc gcatgcaatg cctgtctggg aaaattcaat gcggccataa
     9061 taacatcttc ttcataaccg gcctttttca tagccccgcg caccgccgta tcctgcgcgc
     9121 gataaagcgg atcaccgatc aaaggatgct taagtgcctg catatgcacc cgaatctgat
     9181 gcgttcgccc gctctcaagc ttacattcca ccaggctaag cgcctctcca taagctttca
     9241 aaacccggta atgcgtacgc gccggcttgg cattttgccc cttcacactc atttttaagc
     9301 gatttcgagg gtcccgccct atcggcatat ccacaacacc cttaaccggc accggaactt
     9361 taagcgccaa agcctcataa atccggctta aagaccggtc ctcaagttgc gccgccagcc
     9421 cctgatgcgc ccggtcgtgc ttggccacca ccatcaaccc gcttgtatct ttatcaagcc
     9481 gatggacaat ccccggacgc gccacaccgc caatccccga caggctttcc ccgcaatgat
     9541 ataacaaagc attgaccaaa gtcccgctgg cattacctgc ccccggatgc accaccaacc
     9601 cggccggctt attaatcacc agcacgtcct catcttcata aataacatta agagcgattt
     9661 tttcaggttg cggggccgcc tcttccacag gaggaatctc aatctcaata acgctgccaa
     9721 gcgaaacttt atgcgataca tcacgacaca ccagcccgtt taaatgcaac gcaccgtcct
     9781 gaattatacc ttgcagacgc gaacgcgaaa aaccatccgc taaagcagcc aaagccttgt
     9841 caagccgctg ccccacctgc tcttcactaa ttataaaacg taacgtatct ttttctttat
     9901 ctgagcactc atccatatta cggcatcacc cgctctcttt gccgacaagg atccggatac
     9961 ggatcaaccc ccgtagccgc aaccacccac ccttcctgac tacgcacaac ctcatcggga
    10021 atctccggtc cctgctcatt tatggccctg ctgtgctcaa ttttatatgc aacgcgcgtc
    10081 tcaccaataa tcaaccgctc cgccccttta ctggccatca acgcgtaagc atccaccgct
    10141 tcaccattaa aaaactcgtc actcgaaaac agcacgccac gcggatttaa cgtcaataaa
    10201 cgcacatgct ctatctcttc actgccctca ggagtctgac catcaataag cacactgatt
    10261 aaaccatcct cactcaccat cacatcacgt ccatcaaaat gtaaagagcc gttgagatag
    10321 cgctgccaga ccacatcgcc ggttgcatca ttcacaacca taacccaggc tgcacggttg
    10381 ccgtcaccca tcggcaacgc gctaccgaca accgccagag tatcggcaag catctcatgc
    10441 gcccgtacga tctcagccgc cgcaccgcgc ggataagact tctgccacat catattaaga
    10501 tcctcggcta tgcgcataac ccatcccgtc ttacgcccaa tagcattatc aatagagccg
    10561 ctcgctacat agccgccatc tttcattgga tataaatcat aaacagcatt ttccgaacca
    10621 attacaaacg catgatccgc aatcaccacg ccagcactgt tcacgcggta taataccgac
    10681 caatcaggtt tatcctcttt tggtgatgat gccgacgccg cgatcacaaa agactgacca
    10741 gacgcactgg gctccatatc atgcccacgc aaatgattac ctggatcgcg caccaccttg
    10801 ctgaacagca aatcaccttg taaattgaat accccaagcc agatatgctg ccgtcctttt
    10861 ggaggcgtga tattcgcaag cataaccata cccttatcgt gcggcaaaac cttcacaagc
    10921 tctgccagcc ccggcacctc ataatccttc tcccacaaca cccggccatt gcgcccaatc
    10981 tcgaccaaca acaaatcctt acgatcccct ttagcaggaa gaacgcgctc accggcagcc
    11041 agaaccgtcc ctgtttcgtt aaccaaagca ctcttgaacc gctcatgcgt aggaatttcc
    11101 ccatgcacac tgtcccacac attataggac cccaacgtcg gcgcccgcag ctttataatc
    11161 gcctcatact gaatatcgca gttttgtgcc catacaccgc cagaccacag cgcaaaaaca
    11221 ccgacaaatg cagaaaacaa aaccagcctg tttatcattt tcacgaaacc tttaaagcct
    11281 ttgaaagact ataaaattga cagcgacaaa tgtctatgcc agtaacaaaa gaccattgca
    11341 ggaaagcacg acaaacccga tgaaactctt gattatcagc gatgcctggc acccgcaaat
    11401 taacggcgtt gtgcgcacct atgaacatat tagtcaagaa atgcaaaaac tgggccacac
    11461 tgtcaaagtc atcggcccgg cagattttcc catgagcgtg cccatgcccg gctataaaga
    11521 aatcagactg gccatagccc cctaccgaaa actgcaacgc ataataaaaa aatataaccc
    11581 cgaccatatt caccttccca ccgaaggacc tctcggctgg gctggacgca aatattgcct
    11641 gcgcaatgaa cgtcgctttt caaccgcttt ccacacccgt ttcccggaat atgtcgccca
    11701 gcgcgccgca caaattttgc cctcatcaca cgatttcgtc catgcccgcg tcgtgaatta
    11761 cgtccgtcgt ttccatgccc cctcttccgg catgaccgtc gccaccggaa gccttgaaga
    11821 aacgctgaaa aactggggct ttcaaaaccc gatgcaccgc gtcacgcgca gcgcttgtct
    11881 cgatacattc actccaggag aaaaaacact ttttcatgat ctcaaaggtc cgatcgccct
    11941 gtatgtcgga cgcatcgcca ttgaaaaaaa catcgaagac ttcctcaaaa tggattggcc
    12001 gggaagtaaa gtcattgtcg gcaccggtcc gcaaaaagcc gaactggaac aaaaatatcc
    12061 cgatgcgctc ttcgtcggca aaaaaaccgg cgctgagctt gtagaacact accgctccgc
    12121 cgacctgttt gttttcccct cacgcacaga cacattcggc atcgtcctga tcgaagcgct
    12181 ggcctgcggc ctgccggtcg ccgcttataa tgtaaccggc cccaaagaca ttatcacaaa
    12241 agattttctc ggctctctgc acgacacgaa cttaagcctg gccgcaaaaa ccgctctgca
    12301 aaacaaagac ttggaaaaac gcaccaccca tgtgcataaa gaatatacgt ggcaacatgc
    12361 cgctgcccaa tatgaagaca tgatactgaa taaaatcctc caaacacgat aaaggaaacg
    12421 actatggccc acattctcat tgccgaagac gaccaatcaa tgtgcagctt cctccaactc
    12481 gcgctggaaa aagccggcca taatgtacaa atcgcctata acggcattga tgccctgaaa
    12541 attatagaag aaagcaaccc ccccatcgat ttactcctca gcgatattgt catgcccggc
    12601 atggatggtg ttgagctgtc caacaaagcc acaaaaactc atccttcaat caaaatcatg
    12661 tttatcaccg gctttgccgc cgtagccatg gaacgcgaag acctggaccg ggaaaaaacc
    12721 aaagtcctgt ccaaaccttt ccacctcaac gatctcgtag aacaagtgga aaccttgctg
    12781 tccacctgaa cacactcccg actcagagac ccgaaacact gcaaacacag cgactttcaa
    12841 gcatatccta acactttatt aacgaaattc accgataata aggtgcaaaa ctatatctgc
    12901 tccgcaggtg cagtagaatt gagttaaggg cgtaaaatgg cggaaaacac ccatattgca
    12961 gaggaatata taaaaaaact gggcggtaac aacaccgtct gggcagacac aatcgccccg
    13021 cctaccgccg agcaaaccgc cagattagcc aatttcgcca acgaaggcat gaaaaactct
    13081 ctccttcaag gctatgccgc agacatggga cccgacacta acttttggaa taaaataggg
    13141 acaaaagacg ggtttcacac cgcccccgta gacggaaaaa caggacaatt tcgtttcgaa
    13201 aacctgacac agacaacact tcctacactc ggcggaacag tcggcgccgt tttcgcgcct
    13261 cttgaagaag aatggctggt tggtctggat ttcgaagtca atgtcaaagg cacaattctg
    13321 gtagaaatgg ccaagccggg cgaagaactt ggaccagcag gctataagct ctctcacaac
    13381 gtcaaaacag acttgaactt ctacaacgaa aaagtagagg acccgctaaa agtctcaggc
    13441 aaagccttct ggattgaaaa gagcgaggaa ataccgagct tcgctttcaa acaccaagaa
    13501 gaaacaaacc ttggaaacgc aatggccgac ctcggcaacg cgccagccgg tctgtctttc
    13561 gcctccctcg acacggcaaa gaatccagac atgtcaggcg ttatctccga tacaacaccc
    13621 ctgccgcctg ctgctccagt tgaaaaaagc gttgatccat ttactcagct taacccggaa
    13681 gaagcacata agggttactc agggcctgcc ccggcgctca tcgatgcaaa agtcaccttt
    13741 tccggcgaat atcagatcaa cgctaaaata aatgtcgaag tcgaacacac cttgaccgta
    13801 tcacgtcagg actatgaacg tattaaagcc gaacttgacg gccaaacgat atattccgcc
    13861 agcgttgccg gatccgtaac cgtatggaac cccggtgcag acagcgaagg gaacgccagc
    13921 gaattcttcg ttcacgtagg gcttgataaa cttgagatga aaggcggcat aaacgaaacc
    13981 gtcgccatga ccaaactgct gcaagatgaa aaagacggcg ctggccagca atatattgcc
    14041 gaactcaatg gaagagcacc gcccccggtc aatccatacc ttcaagacag ccccggcaaa
    14101 accatgatcg ccaaggcaag ccccaccggc acagcggatg acgtccggct tgcccaggca
    14161 tcatgcaatc tgctcagcga cgccaacctc gcgattgacg gcgatcccgg caccaaaacg
    14221 cagagctgcg tcggcacagt tctgggcgat gattatgtcg ccgatgattg gaaacaaaac
    14281 gccgaaaatc tgcaggaaat cgtcgccctc agccccgaag ccgcagaaaa aggccggatt
    14341 ctggcaacaa aaaccctcaa cggcgaaata aaggatttct caaaagaagc ctacgaaact
    14401 caggcattcc taaaagccaa agaaggcata gcctttgacg gcgacatcgg ccgaggcacc
    14461 cgcgccgccg caacggccca tctcgacatg gaaaaccagc ccagcatcga agccatcgcc
    14521 aacgcaccgt ccccggggaa aggcactcca tcactcagtt gtgaatttgg aaaagtctgc
    14581 ggcccggaaa acgccgccaa cgatccaagc tttgatcaac cggaaaccga cgctcgggcc
    14641 gttgctaaaa cgcagccccc catacaacca accagagagg tggtctcggt tcttcggcca
    14701 gctttgcggc gaagttaagc tacgatcg
//
LOCUS       DAOSWR010000045        14324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2677_length_14324_cov_5.749667, whole genome shotgun sequence.
ACCESSION   DAOSWR010000045 DAOSWR010000000
VERSION     DAOSWR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_14324_cov_5.749667"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..486
                     /locus_tag="PK513_04830"
     CDS             91..486
                     /locus_tag="PK513_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81805.1"
                     /translation="MALYGLHCKDFDHFSTLFCRDFNLKSLIIKRLSSDSQEKSFYLA
                     SITTQHNNSVCARSDNLSGLTTLLSNKILSDLIAVEFGLNRDSSAQNLLGAASKLATI
                     GRVLDNNFIPEEKLKRIKKLEKPETINRM"
     gene            710..1243
                     /locus_tag="PK513_04835"
     CDS             710..1243
                     /locus_tag="PK513_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOO81806.1"
                     /translation="MANDNFDFTAGTSVVYPAHGVGVIEGIETQNIGGMEVSLYAISF
                     EKDRMRLKIPVMKAEAAGLRKLSSTDRMGDALKTLKGKARVRRTMWSRRAQEYEMKIN
                     SGDPVAIAEVLRDLKRSNDDSEQSYSERQIYQSALERLAREVAAVEKITEGKAIERLE
                     DIMGVPKEMPLEDKQAA"
     gene            1418..2233
                     /locus_tag="PK513_04840"
     CDS             1418..2233
                     /locus_tag="PK513_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81807.1"
                     /translation="MVWKDSDSISSMGTPHIVWSIPAIHADLVQLVRLHDMIFSRIKP
                     GDRIIYHGNYTGYGERAVACLDEILAFRRMVLALPGMLPSDIVYLRGQQELIWEKLLQ
                     IQFAPDPTNVLLWMLGNGLTNTLYDYGLSPHDGIDACKTGIMGLTKWTNSIRKALRAH
                     PGHETFMNQLKRMAVTDKSAPAPMLFVHAGLNIERALHEQGDDIWWATDNFRKIEHPY
                     APFTKVVRGFDPDHGGMYLNGVSATVDDGCGFGGQLISVGFDPDGAAAHILEA"
     gene            2370..3266
                     /locus_tag="PK513_04845"
     CDS             2370..3266
                     /locus_tag="PK513_04845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOO81808.1"
                     /translation="MTYIDDPQTQKANMDYIRCSMGEPLLEKGHELDLARQWRDKKDE
                     RALHKLVKSYTRLVISVASRFRNYGLPMGDLIQEGNVGLMLAAERFDPERDIRFSTYA
                     TWWIRASIQDYVLRNWSIVRTGTTSAQKSLFFNYRRLRAKVERAADHEGLNDDSRVQI
                     ANELNIKVKDVEAMEGRLSGTDQSLNAHIGQDGEDEWQSLLSDERPNPEEVALHVVDS
                     EARSRWLNNALHDLSDREQTIIRQRHLGQEVVTLEALGKELGVSKERVRQLEVRAMEK
                     LKTSIENQADIGPELFSGGLPY"
     gene            complement(3357..4763)
                     /locus_tag="PK513_04850"
     CDS             complement(3357..4763)
                     /locus_tag="PK513_04850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOO81809.1"
                     /translation="MTAALTGCSTNPATGQQQFTALMSPQQEVQVGASEHQKIAQQFG
                     FYEDARVNAYVTSVGRKVTTKTERPDVQYKFFVLDSPIVNAFALPGGYIYVSRGLLAL
                     ANSEAELAAVLAHETGHITGRHSAERYSRGVVTTLGAGVLSAVLGSDGASQALGVGAN
                     LYLSAYSRGQESEADALGLRYMTHGGYDVDAMAKFLSSLKAQSDLDSRLAGNGNANGF
                     SYFSTHPATADRVAQTRTQARNYPSGGQVNRNAHLNIINGMIYGDSAAQGFVRGQSFY
                     HPKMDFTFTVPAGFNLKNSPAQITASASNGAVLIFDMAANKQGLDPRSYVQSWVDDRQ
                     LQGLESINVHGRTGATASFAGNINGRPVTIRLVAIAYGDRFARFQMAIPAGAPAAQLE
                     GLKAATYSFRSMSDKEKNSVHPYRLQTFIAQSGDSVASIVRRQPFTNLQEERFLVLNG
                     LRAGEPLQTGWMYKKIVE"
     gene            4827..5690
                     /locus_tag="PK513_04855"
     CDS             4827..5690
                     /locus_tag="PK513_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOO81810.1"
                     /translation="MFLKNWHILVFFLLLANLYSIGAAYGQDASALPASDFSALRKTG
                     EAQVLEVISPLTVKLSNGDIVRLPGLHFPDYDYTAERTGPFALTAMKVLQDMLEGKKV
                     EIYQTPKKDWGRTNRMGHALAHLVRSDSGLWAQGMLIRLGLAQVKTSQRNPEMAAQLY
                     ALEAEARHEKIGIWENDDVILSPEDALKHLNSFQIVEGVIQSAAMKKNRVYLNFGGNW
                     RNDFTVSIAPEDRRIFSKAEINPLDWNGAHVRVRGWVEEYNGAYIEIDHPEALEILSA
                     GDDVQKRPNDK"
     gene            5763..6776
                     /gene="pip"
                     /locus_tag="PK513_04860"
     CDS             5763..6776
                     /gene="pip"
                     /locus_tag="PK513_04860"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOO81811.1"
                     /translation="MPHRKHKPVSQTTNDVSRTPRKPLLDLFPALTPYSSGFLDVDDT
                     HNLYWEQSGNPDGAPVLFLHGGPGAGATPVHRRFFDPDHYRIIIFDQRGSGRSHPLGS
                     LENNTTVHLVDDIERLRQHLRIERWHIFGGSWGSTLSLSYARVYPQRCISMILRGIFL
                     CEQDEIDWFLYGMRNIFPEAWEQFAGFLPEDEQSDLLDNYYKHLTGADEEKQMEAAIH
                     WSLYEGACASLLPNYETITTDEQKDHALALARIEAHYFKYEVITRENSLLNSIDKIRE
                     IPSTIIHGRYDLICPIRTAHKLHQLWPEADYIVVPDAGHSALDPTLRSRLIEATERAK
                     TLA"
     gene            6820..8025
                     /locus_tag="PK513_04865"
     CDS             6820..8025
                     /locus_tag="PK513_04865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO81812.1"
                     /translation="MSFQSIRDMDFQGKKVLLRADLNVPARRGKVTDTTRIDRLKPTI
                     DFLRGAGAKILILSHFGRPEGEQNPEMSLAFLTPTLEERWGCNIRFSPDCIGVKAEAV
                     IAKMCEGDVTLLENVRFYKEEKQNAPEFVAKLAALGDVFVNDAFSAAHRAHASTEGLA
                     HVLPAVAGLLMEQELNALDAALEHPKKPVAAIVGGSKISTKLGVLNNIVQRVDYMILG
                     GAMANTFLYANGADVGSSLCETNMKDEALRIFETARKGGCEIVLPIDSVVVSELKLNA
                     PSEIVDSQAIPNDKMAIDVGPKSIAYVESILEKSKTLLWNGPMGVFELKPFDHGTNAL
                     ARKAAEQTKTGNLVSIAGGGDTVAALENAEVCQDFTYISTAGGAFLEWLKGETLPGIE
                     ALNITQKAA"
     gene            complement(8026..9159)
                     /locus_tag="PK513_04870"
     CDS             complement(8026..9159)
                     /locus_tag="PK513_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HOO81813.1"
                     /translation="MDLSFLDKTSTVTPLLVRLYDSHKLYGLASDDKPLARAELTSAV
                     SELLEMELSPKESELIADVLIALMRQAEIDLREALSERLAILENVPLRLILQMANDEI
                     PVAAPVLSKSPVLGDMDLIYIIKSKGPKYWQAIAKRENMSNQIMNLLADTRDIDTTIQ
                     LVKNKKINLTEYSLTILSDLAQQSDALAAPLLRREEVGPELAKVLYAYVGEEIKEQVK
                     TLYGFEAQSVVQAVDEVIVEMVEASESVSEFTPNKAILRAADRYKQKGLLTIKLMLGT
                     LRRGQIAAFVGQFSRYSGLDPKTTLEILSQTSGQGLAVACKAFDVPKEDFISIFLLTN
                     RVRNHGRMVALNDMTKAINYFNRIKPEVARSIIENSLDEELKK"
     gene            9447..9734
                     /locus_tag="PK513_04875"
     CDS             9447..9734
                     /locus_tag="PK513_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81814.1"
                     /translation="MAGPLSGIAGQQQVPLAQPSQTSTQNTNGVRGQSEQKPEADRVQ
                     PQGAPAAETQNTETHNEDVLQQQIQNALTTDQNDPAGAETLRRGSLVDVVI"
     gene            complement(9837..11255)
                     /locus_tag="PK513_04880"
     CDS             complement(9837..11255)
                     /locus_tag="PK513_04880"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOO81815.1"
                     /translation="MMHSPDMIAPKTCSAPEEVLAQVKIIYDAQIAFLRARFDALTKG
                     DGVKGRSRAFYPYARISTKYARRTDTRLSYGFAPRPGTYSITLTRPDIFANYYLEQFR
                     NIIKNHDITIEVGVSNTPIPLHFALGEEFHLEGDLGAEQLKDLPDLFDMPNLDIMDDE
                     IANGTYIPAEGQDRPLALFTAPRVDLSIQRLRHYCGMPAEHFQNFVIFTNYQFYIDEF
                     VKIGESKAGKDGYSEFIQPAAATRLPQMPAYHLKRKDGKGITMVNIGVGPSNAKTITD
                     HIAVLRPHAWLMLGHCAGLRNSQNLGDYVLAHGYLREDNILDTDLPTSIPIPALAEIQ
                     VALEKAVSEICGFEGYDIKKVMRTGTVATTDDRNWELRSSIRQNKRLSQSRAIAMDME
                     SGTIAANGFRFRVPYGTLLCVSDKPLHGQLKLPGMANTFYRQRVDQHLQVGIRAMEML
                     RQLAPEKLHSRKLRSFNEVAFQ"
     gene            complement(11287..12471)
                     /gene="ftsY"
                     /locus_tag="PK513_04885"
     CDS             complement(11287..12471)
                     /gene="ftsY"
                     /locus_tag="PK513_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOO81816.1"
                     /translation="MVFWRKKQNIAEQEQEEREEKLFHPKGEPAIEPSTDYEAELDDE
                     IRHEMEDETASEILDDLRLSPAPKHDRLDDAEEAEEFADDSNEGGWLSRLTSGLSKST
                     NKITKGLTDLVTKKTLDQETLDGLEELLIEADLGPKTAAKIIEEFSKGRFGKEISEEE
                     IREALAETIAGILKPVAKLLTIEKPDDGPFTIIVCGVNGVGKTTTIGKIAQNLKFKDH
                     CSVVMAAGDTFRAAAVEQLGIWAKRTGAKLVQKDVGADAASVAFEAYEQAKNQGADVL
                     LIDTAGRLQNKANLMDELAKVIRVLKKQDESLPHETLLVLDATTGQNAFSQLETFKDM
                     VNVTGLIVTKLDGSAKGGVLVGLADQFGVPVYSIGIGEGLDDLQPFCPREYARALMGL
                     EN"
     gene            complement(12507..13760)
                     /gene="mtaB"
                     /locus_tag="PK513_04890"
     CDS             complement(12507..13760)
                     /gene="mtaB"
                     /locus_tag="PK513_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOO81817.1"
                     /translation="MDQKSPKLVTFGCRLNTYESEVMRTHAANAGLDDAIIFNTCAVT
                     KEAERQARQAIRRARRENPNAKIIVTGCSAQITPDTYAAMPEVDQIIGNDLKLKAESW
                     GVEQGEKVMVNDIMSVTETASHLIEGFEDRSRAFLQVQNGCDHRCTFCIIPYGRGPSR
                     SVPIGVIAEHVRALVEQGYNEIVFTGVDVTSYGPDLPGAPTLGQMIRRVLALVPELPR
                     LRLSSLDPVEIDDDLWRLIAEEPRLMPHLHLSLQAGDDMILKRMKRRHLRNDVIAMCQ
                     RARTLRPDIEFGADIITGFPTETEEMFQNTLKIVEECDLTYLHVFPYSERKGTPAARI
                     PGQVPPKERKERAARLRALGELQLQKLLRRNLNKEAQIIVEKGSFGRTQNFSPVHLNA
                     KARPGDLLSVKLSGITGENLLGEPL"
     gene            complement(13771..>14324)
                     /gene="dapF"
                     /locus_tag="PK513_04895"
     CDS             complement(13771..>14324)
                     /gene="dapF"
                     /locus_tag="PK513_04895"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO81818.1"
                     /translation="DQCAIETGRGVLDCSAVENGLVTVDMGAPQLDWAAIPLAEERDT
                     LMVELGTDTHNPAVAVGMGNPHCVLFVDDAENALVERFGPVIENHALFPERTNVEFVQ
                     LLEDGRLRQRTWERGCGETLACGSGACAVAVATIRRGLIDGRKVEILLNGGPIHIEWR
                     ESDNHVLMTGPVAYIFDGRLTNL"
BASE COUNT         3533 a         3337 c         3720 g         3734 t
ORIGIN      
        1 cgatcgtagc ttaacttcgc cgcaaagctg gccgaagaac cgagaccacc tctgagcagc
       61 ctgataaaaa ttacaggata tatttcgtac atggcccttt acgggctgca ttgcaaggat
      121 tttgatcatt tttcgacgct gttttgtcga gatttcaatc ttaagagcct aataattaaa
      181 cggctttcct cagatagtca ggaaaagagc ttttatcttg ccagcattac cacacaacac
      241 aataattcgg tgtgtgcaag atctgataat ttatccggcc tgacgacttt gttatcaaat
      301 aaaattttga gtgatcttat cgcggtagaa tttggattaa atcgcgatag ttctgcacaa
      361 aatttattgg gtgcggcgtc taaactagca acaatcgggc gcgttctgga caataatttt
      421 attccggaag aaaagttgaa acgtataaaa aaacttgaaa aacctgaaac tatcaacagg
      481 atgtaaagtt attaaaaaca gtacattggc tctgtttgtt aataaaaatt tttaagactt
      541 tattaagttt ttgttgaaaa tttgtgaaat tttaggtacc atctggcttc acgtttaaac
      601 aagacattgt tttgtgaatg gcgaaagtgc ttatccgggg cggactcggt gcgctgtggt
      661 gtgggccttt tgtcaataaa cataacaaca aaaagaaggt gcattgaaca tggctaatga
      721 taattttgac ttcactgctg gaacctccgt tgtataccct gcacatggcg taggcgttat
      781 cgaagggatc gaaacccaga atattggcgg catggaagtc agcctttatg ctatttcttt
      841 tgaaaaagac cgtatgcgcc ttaaaattcc tgtgatgaaa gccgaggctg cgggcctgcg
      901 caaactcagc tctacggatc gtatgggcga tgcgctaaaa acactgaaag gcaaagcccg
      961 cgtacggcgt acaatgtgga gccgccgcgc acaggaatac gaaatgaaga ttaattccgg
     1021 tgatcccgtc gcgattgcag aagttctgcg cgatctgaaa cgtagtaatg acgatagcga
     1081 gcaatcttac agcgaacgtc aaatctatca atcagcactg gagcgtttgg cacgcgaagt
     1141 ggcggcggtt gagaaaatta ccgagggcaa agcaatagaa cgccttgaag acattatggg
     1201 tgttccaaaa gaaatgcctc tggaagacaa gcaagccgct taagagtggg cttctcttac
     1261 atttacctga aattttaatc gggcgcatgg ataagttttt aggctttcta tgcgcctgat
     1321 tttattatat actgactttg attctgggaa ctcttcccgc cctcaccttt tttcggtcac
     1381 cgaaaagtca cccacgagaa cgaacgcaaa cgtgaatatg gtttggaaag attctgattc
     1441 tatctcctca atgggcacac cccatatagt atggagcatt cctgctattc acgcagatct
     1501 agtacagctt gtgcgcttgc atgatatgat ttttagtcgg atcaagccgg gtgaccggat
     1561 catttatcat ggtaattaca caggctatgg tgagcgtgcg gtagcgtgcc ttgatgaaat
     1621 tctggccttt cggcggatgg tgttggcgct gccggggatg ctgccgtctg atattgttta
     1681 tctgcgcggg cagcaggaac ttatctggga aaagctgtta cagattcaat ttgcgcccga
     1741 cccgaccaat gttttgctgt ggatgttagg gaacggtctg accaacacgc tttatgatta
     1801 tgggctgtcg ccgcatgacg ggattgatgc ctgcaagacc ggtattatgg gcctgacgaa
     1861 atggacaaac tcgatccgca aggcgctgcg agcgcatccg ggccatgaaa cctttatgaa
     1921 ccagctcaaa cgtatggctg ttacggataa gagcgcccct gccccgatgc tgtttgttca
     1981 tgcagggcta aatatcgaac gcgccttgca tgagcaaggc gatgatattt ggtgggcaac
     2041 ggataatttt agaaaaatag agcaccctta cgcgcccttt accaaagttg ttcgcggatt
     2101 tgatcctgat catggcggca tgtatcttaa cggcgtcagc gccacggttg atgacgggtg
     2161 cggctttggc ggacagttaa tcagcgtcgg gtttgatcct gatggcgcgg cggcgcatat
     2221 acttgaggcc tagggctttt tgagcctgcc gcatcgacga atttcaaaaa aaatattact
     2281 ttttgttaac ttttttgtgt tttgagcgaa tgcaaatgca agcggcatac gtaagtttta
     2341 cgtgcctaca taaaaggagg atgaatttta tgacgtatat tgatgatccg cagacacaaa
     2401 aagcgaatat ggattatatt cgctgttcta tgggtgagcc tttgttggaa aaaggccatg
     2461 agttggactt ggcgcggcaa tggcgtgaca aaaaagatga gcgcgcgctg cataagcttg
     2521 tgaagtctta tacacggctg gtgatttcag tggcctcgcg ctttcgtaat tatggcttgc
     2581 cgatgggtga ccttattcag gaaggcaatg tcgggttgat gctggccgct gagcgttttg
     2641 atccggagcg tgatattcgt ttttcgacct atgcgacgtg gtggatccgc gcgagcatcc
     2701 aggattatgt actgcggaac tggtcgattg tacgcaccgg cacgacatcg gctcagaaat
     2761 cgctgttttt taattaccgc cgtttgcgtg caaaggttga gcgcgccgcc gatcatgagg
     2821 gccttaatga tgatagccgg gtgcaaattg ccaatgagtt gaacatcaag gtcaaggatg
     2881 tagaggctat ggaaggccgt ttgtctggca cagatcaatc gctgaatgcc catattggtc
     2941 aggacggcga ggatgagtgg cagagcctgc tgagtgatga gcggccaaac ccggaagaag
     3001 ttgcgttgca tgttgtagat tctgaggcgc gatcgagatg gttgaacaat gcactgcacg
     3061 atctgagtga tcgggagcag actattatcc gccagcggca tttgggacag gaggtcgtga
     3121 cgcttgaggc gcttggtaaa gagttgggcg tgagtaagga gcgtgtgcgc cagcttgaag
     3181 tgcgagcgat ggaaaagctg aaaacatcaa tcgaaaacca agccgatatc ggcccagaat
     3241 tgttttccgg cggactaccg tattaaacct taaaggtttc ctttccccag ctgcactaac
     3301 tgatcggcac ctctcttccc cttaagtgtc ggtcagtttt tatgcggata ttcgatttat
     3361 tctacgattt tcttgtacat ccatccggtc tgcaacggct cgcctgccct caagccgttg
     3421 aggacaagga aacgttcttc ctgaagattt gtaaaaggct gacggcgaac gattgatgct
     3481 acgctatcgc cggattgggc gatgaacgtt tgcaagcgat atggatgcac agagtttttc
     3541 tctttatcgc tcatagagcg gaagctatag gtcgccgcct ttaagccttc gagctgcgcg
     3601 gctggagcgc ccgccggaat cgccatctgg aagcgggcaa agcgatcacc atatgcaatc
     3661 gccaccaagc ggatcgtcac aggacggcca ttaatatttc cggcaaaact agccgttgcg
     3721 cctgtgcggc catgtacatt gatggattca agaccttgca actggcgatc atcgacccag
     3781 ctttgcacat agctgcgcgg gtcaagccct tgcttatttg ccgccatatc gaagatcaga
     3841 actgcgccgt tgcttgcgct cgcggtgatt tgggcggggc tgtttttcaa attaaagcct
     3901 gcgggcacgg tgaaggtaaa gtccattttc gggtgataaa aactttggcc gcgaacgaac
     3961 ccttgggcgg cgctatctcc ataaatcatg ccgttaatga tattgaggtg ggcattccga
     4021 ttcacctgcc cgccactagg ataattgcgg gcttgcgtgc gcgtttgcgc aacgcggtcg
     4081 gcggtggccg ggtgagttga aaaatagcta aagccgtttg cattaccatt gcctgccagg
     4141 cggctgtcga ggtctgattg tgcctttaat gaactcagga atttagccat cgcatctaca
     4201 tcgtaaccgc catgagtcat gtagcgcagc cccagtgcgt cggcttcgct ttcctgccca
     4261 cgggaatagg cgcttaagta gaggttcgcg ccgacgccca atgcttgcga ggctccatca
     4321 ctacccagca cagcggagag cacccctgcg cccagtgtgg taactacgcc gcgtgaatag
     4381 cgctctgcgg aatgacgccc ggtgatgtgt ccagtttcat gggccagtac ggcggcgagt
     4441 tcggcctcac tgtttgccaa tgccagcagt ccacgcgaaa catagatata gccgcccggc
     4501 agtgcaaaag cgttgacgat gggactgtcg agaacaaaaa atttatactg aacatcgggg
     4561 cgttcggttt tggtggtgac ctttcgaccg acgctggtga cgtaggcatt aacccttgca
     4621 tcttcataaa agccgaattg ctgggcgatc ttttgatgct cggatgcgcc aacctgcact
     4681 tcttgctggg gcgacatcaa agcggtgaat tgttgctgtc ctgtggcagg attggtggag
     4741 caccccgtca gtgcggcagt caaaaaaacg tttaaaactg caaagaaaac cttgaaacgt
     4801 cgcatcacat ccatccactg ttagttatgt ttcttaaaaa ctggcacatc ttagtctttt
     4861 tcctgctgct cgccaactta tatagtatcg gcgctgcgta cgggcaagac gctagcgctc
     4921 tgcccgccag cgatttttcc gcgctcagaa aaaccggcga agcacaagtg ctggaagtca
     4981 tcagcccgct tacggtaaag ctcagcaacg gcgatattgt ccgcctgccg ggactacact
     5041 tcccagacta tgattatacg gcggagcgca ccggcccctt cgctcttact gccatgaagg
     5101 tccttcaaga tatgctggaa gggaaaaaag tggagattta tcaaacgccg aaaaaagatt
     5161 gggggcggac caaccgcatg ggccatgcgc tggcacatct tgtacgatct gatagcggat
     5221 tgtgggcgca aggaatgctg atccgccttg gcttggcgca ggtcaaaaca tctcagcgca
     5281 acccggaaat ggcggcccag ctttatgcgc tggaggcgga agcccgccac gagaaaatcg
     5341 gcatttggga aaatgatgat gtcattttga gcccggaaga tgcgctgaaa catctcaaca
     5401 gctttcagat tgtggagggt gtcattcaaa gcgcggcgat gaaaaagaac cgggtttacc
     5461 ttaattttgg cgggaactgg cgtaatgatt tcactgtatc gattgcgccg gaggaccgaa
     5521 gaatattttc gaaggctgag attaatccgc tggactggaa tggtgcgcat gtgcgggtgc
     5581 gtggctgggt tgaagagtat aatggcgcgt atattgaaat tgatcatccg gaggctctgg
     5641 aaattttaag cgcaggcgat gatgtacaaa aacggccaaa cgacaaatag attgcctcgt
     5701 gatacctgaa gtcttaagct ggtgcaatcg taaacatttt aatattttgc ctgaatttgc
     5761 cgatgccaca tcgtaaacac aagcctgttt ctcaaaccac aaatgatgtt agccgcacgc
     5821 ctcgaaagcc tttactcgac ctgtttccag ccttaacgcc gtattcaagt ggttttttgg
     5881 acgtcgatga cacgcataat ctttactggg agcaaagcgg taatcctgat ggggcgcctg
     5941 ttttgttttt gcatggcggt cctggtgctg gggcaacgcc ggtgcatagg cgtttttttg
     6001 atccggatca ttaccggatt ataattttcg atcagcgcgg atcggggcgt tcacaccctt
     6061 tgggcagtct tgaaaacaac acaactgtgc atctggttga tgatattgaa cggcttcgcc
     6121 agcatttgcg tattgagcgc tggcatattt ttggcggatc gtggggcagc accttgtcgc
     6181 tgtcttatgc ccgcgtatac ccacagcgct gcatcagcat gattttgcgc gggatttttt
     6241 tatgtgagca agatgagatt gactggtttc tttacggcat gcgcaacatc ttccccgagg
     6301 cgtgggagca gtttgcgggt tttttaccgg aagatgagca aagtgacctg ctggataatt
     6361 attacaagca tttgactgga gcagatgaag aaaaacaaat ggaagcggct attcactgga
     6421 gtctttatga aggagcatgc gcatcgctgc tgcccaatta tgaaaccatt acaacggatg
     6481 aacaaaaaga ccatgcgctg gcgctagcgc ggatcgaggc gcattatttt aaatatgaag
     6541 tgattactcg tgagaacagt cttttgaaca gcattgataa aatccgggaa atcccctcaa
     6601 ctatcattca tggacgttat gatctgattt gtccgatccg aactgcgcac aaacttcatc
     6661 agctttggcc ggaggcagat tatattgttg tgcccgacgc cggacattcg gcgcttgatc
     6721 ctacgctacg ttcgcgcttg attgaagcaa cagaacgtgc caaaaccctc gcatagaaaa
     6781 acaaacacga aaatgaatag cgacaaaaac gagacaaaga tgagttttca atcgatccgc
     6841 gatatggatt ttcagggtaa aaaagtcctt ttgcgcgcgg atttaaatgt gccggcgcgg
     6901 cgtggtaaag taacggatac tacacgcatt gaccgtttaa agccgacgat tgattttctg
     6961 cgcggggctg gtgcaaaaat tcttattctt tcccattttg gccgcccgga aggagagcaa
     7021 aacccggaaa tgtcgttggc ctttttgaca ccgactctgg aagaacgctg gggatgtaat
     7081 attcgtttta gccctgattg cattggggta aaggctgaag ctgtaattgc aaagatgtgc
     7141 gaaggcgatg ttacgctgtt ggaaaatgtt cgtttttaca aagaagaaaa gcaaaatgcg
     7201 ccggaatttg ttgcaaaact ggcggcgcta ggggacgttt tcgtcaatga cgctttttcc
     7261 gcagcgcacc gcgcccatgc aagtacggaa ggcttggcgc atgttcttcc agcggtagcg
     7321 gggcttttga tggagcaaga gctgaatgct ctcgatgcag cgttggaaca cccgaaaaaa
     7381 ccagtagcgg caattgtcgg cggttcaaaa atttccacta aacttggagt gctcaacaat
     7441 atcgtacagc gggtggatta tatgatcctc ggcggggcaa tggccaatac atttctttat
     7501 gcaaatggcg cggatgtagg tagctccttg tgtgagacca acatgaagga tgaagctcta
     7561 agaatttttg aaacggcacg caagggtggc tgtgagattg tactgccgat tgatagtgtg
     7621 gtggtgagcg agctcaaact caacgctcct tctgaaattg ttgattctca ggccattccg
     7681 aatgataaaa tggctattga tgtcggtccg aaaagcattg cctatgtcga aagcattctt
     7741 gaaaaaagca agactttgct ctggaacggg ccaatggggg tatttgagct taaacctttt
     7801 gaccacggta ccaatgcatt ggcgcgcaag gctgcggaac aaacaaaaac cggaaatctg
     7861 gtcagtattg ccggaggcgg cgatacggtc gctgcgctgg aaaatgccga agtctgtcag
     7921 gatttcactt acatttctac tgcgggcggg gcatttttag agtggcttaa aggcgagaca
     7981 ctgcccggca ttgaggccct caatataact caaaaagccg cctgattatt tcttcaattc
     8041 ttcatcgaga gaattttcaa tgatactgcg tgcgacctca ggtttgatcc tgttgaaata
     8101 atttatcgct tttgtcatat cgttcagcgc aaccatgcgg ccgtggttgc ggacgcggtt
     8161 tgtcagtaaa aaaattgaaa taaaatcttc ttttggaaca tcaaaagcct tacacgctac
     8221 ggccagcccc tgcccgctgg tttgtgaaag aatttccagc gtggtcttcg gatctaaacc
     8281 agaataacga gagaactgtc caacaaaggc tgctatttgc ccgcggcgta acgtgccgag
     8341 catgagctta atggttagca gtcctttttg cttataacgg tctgctgccc tgaggatagc
     8401 cttgttgggc gtaaattcgc tgacggactc gctggcctca accatttcaa caatgacttc
     8461 atcgacggcc tgaactacag attgggcttc aaaaccgtag agtgttttga cttgttcttt
     8521 tatttcttca ccaacatatg catataggac tttggccaat tccgggccga cctcttcacg
     8581 ccgcagaagc ggagcggcca gtgcgtcaga ttgctgggcc aagtcgctaa ggattgtcag
     8641 actatattcg gttaagttaa tttttttatt tttaactagt tgaatcgtcg tatcaatatc
     8701 acgcgtgtca gccagcaagt tcataatctg gttgctcata ttttcgcgtt tggctatagc
     8761 ctgccagtat ttcggcccct tggacttaat gatataaatt aaatccatat cccccaagac
     8821 cggactcttg cttaaaacag gcgctgcgac aggaatttcg tcattggcca tttgcaaaat
     8881 gagacgtagt ggcacatttt ctaaaatagc gagacgttca gataaagctt cccgcaggtc
     8941 tatttctgcc tggcgcatca aggcaatgag tacatcggcg atcaattcgc tttctttagg
     9001 agaaagctcc atctccagta attcgctaac agcagaggtc agttcagcac gcgcaagagg
     9061 cttgtcgtca ctggccaaac catacagctt gtgcgaatca tatagtcgca ccaataaagg
     9121 tgttactgtt gatgtcttat ctaaaaaact tagatccatg agatgcccca gactgcgtca
     9181 tatttttatt ttatttttat ataagaaaac aatgaagtac tttgttattc atagcatttt
     9241 agctaaattt tatactgtta tttccgttct ctgcctctat atatacgggg tatttcttac
     9301 gtttcagtta ataaaaaatt accaaaaaat taatgtatgc aatctctcgt gtttgattta
     9361 tagactctgc acccttatac tgttttttaa cgcttatgaa gtaccctata aattggggtt
     9421 tcattttgta aaaaggatac aatcttatgg caggaccatt aagcggtatt gcgggacagc
     9481 agcaggttcc cctggctcag ccctcacaga cgtccactca aaatacgaat ggtgttcgtg
     9541 gtcagagtga gcagaagcca gaagctgacc gggttcagcc acaaggtgca cctgcagctg
     9601 aaacgcaaaa tacggaaaca cataatgagg atgttctgca gcaacaaatt caaaatgctt
     9661 tgaccactga ccagaatgat cctgccggtg ctgagacgct gcggcgcggt tcgttggttg
     9721 atgtggtgat ttaagcatag agattttacg ttcgtgggga tgggcgcttc tttttgaagc
     9781 gccttttcga ttctggccga ggcgtttcta attagggggg caaaacctaa gtgcgcttat
     9841 tgaaatgcga cttcgttgaa gctgcgaagt ttgcggctat ggagtttttc tggggccaat
     9901 tggcgcagca tttccatggc ccgaataccg acctgtaagt gctggtctac gcgctggcga
     9961 taaaacgtat tggccatgcc cggcagcttg agttgcccgt gcagaggttt gtcggagaca
    10021 catagaagcg tcccataggg tacgcgaaaa cgaaaaccgt tggcagcgat tgtaccgctc
    10081 tccatatcca tggcgatggc gcggctttgg ctgaggcgtt tattttggcg gatggaagag
    10141 cgcagttccc aattacggtc atcggttgtg gcgactgtgc cggtgcgcat gacttttttg
    10201 atgtcgtatc cctcaaagcc acaaatttcg ctcacagctt tttccagcgc gacttgaatt
    10261 tccgccagag cgggaatagg aatgctggtc ggcaggtcgg tatcgaggat attgtcttcg
    10321 cgtaagtagc cgtgggcaag aacataatca ccgagatttt gagaattgcg cagtcctgcg
    10381 caatggccga gcatcagcca cgcatgcgga cgcagtacag cgatgtggtc ggtgatggtt
    10441 ttggcattgg aagggccaac accgatattg accatggtga tgcccttacc atctttgcgt
    10501 ttgagatgat aggccggcat ttgtggcaaa cgtgtggctg cggcaggctg gataaattcg
    10561 ctataaccgt cttttcctgc tttgctctcg ccgattttaa cgaattcatc gatatagaat
    10621 tgatagtttg taaagatcac aaaattctga aaatgttctg caggcatgcc acaataatgg
    10681 cgcagacgct gaatgctgag atcaacgcgc ggcgcggtaa agagcgcgag cggtcgatcc
    10741 tgcccttctg cgggtatata cgtgccatta gcgatttcat cgtccataat atccagattc
    10801 ggcatatcga atagatccgg caggtcttta agctgttctg ctcccaaatc gccctcaagg
    10861 tgaaattctt cacccagcgc gaaatgcaat ggaatcggcg tattgctgac accgacttca
    10921 atcgtaatat cgtgattttt tatgatatta cgaaattgtt cgagatagta attggcaaaa
    10981 atatccgggc gggtcagggt tatgctatat gtgccggggc gcggagcaaa gccataggac
    11041 agcctcgtat ccgtgcggcg tgcatatttg gtcgaaatgc gcgcataagg gtaaaatgca
    11101 cggctgcgtc cctttacccc atcgcctttg gtcagcgcgt caaagcgggc gcgcaggaag
    11161 gcaatctggg catcatagat aattttgacc tgcgccaaaa cttcttcagg cgccgaacag
    11221 gttttggggg cgatcatatc gggagagtgc atcatccttt tattatcgca gattttttgt
    11281 tatttatcaa ttctctagac ccataagtgc gcgggcgtat tcacgcggac agaatggttg
    11341 taaatcatca agcccttcac caataccgat agaatagacc ggtacgccga actggtcggc
    11401 cagcccaacg agaacaccac ctttggcgct gccgtcgagc ttggtgacga tcaggccggt
    11461 gacattgacc atgtccttga aggtttcaag ttgagaaaat gcattttgac cggtggtggc
    11521 atcaagcact aaaagcgttt catgcggcaa gctttcgtcc tgttttttta gtacgcggat
    11581 gactttggcc aactcatcca tcaagtttgc tttgttttgc aagcgtccgg cggtgtcgat
    11641 cagaagcaca tcagcccctt ggtttttggc ctgctcataa gcctcaaaag ccacagaagc
    11701 ggcatctgca ccaacgtctt tttgtaccaa cttcgcgccg gtgcgcttgg cccaaatgcc
    11761 gagctgttcg acagcggccg cgcggaaagt atccccggca gccatcacaa ccgaacagtg
    11821 atctttaaat ttcaaattct gggcaatctt accaatcgtg gtggtcttac ccacgccatt
    11881 cacgccgcaa acgattatag taaaaggtcc gtcatccggt ttttcaatag taagcagttt
    11941 ggcgacaggc tttagaattc ctgcgattgt ttcggccaga gcctcacgga tttcttcttc
    12001 actaatttcc ttgccaaagc ggcctttaga aaattcctcg ataattttgg cagcggtttt
    12061 tggaccaaga tccgcctcga taagcaactc ttccaacccg tcaagtgttt cttgatccag
    12121 cgttttcttt gtaaccagat cggtgagtcc tttggtgatt ttgttggtgg atttggacaa
    12181 accactggtc aggcgtgaaa gccaaccgcc ttcattactg tcatcggcaa attcttcggc
    12241 ctcttccgca tcgtcaagcc tatcatgctt aggcgcagga gacaggcgca gatcatcaag
    12301 aatttcactg gcggtttcat cttccatctc atgacgaatt tcatcatcca gttcggcttc
    12361 ataatctgtg gatggctcaa ttgccggttc acctttggga tgaaaaagtt tttcttcacg
    12421 ttcttcttgt tcctgctcgg caatattttg ctttttacgc caaaaaacca tttcaatctc
    12481 cttgtgttat ggagattata cgccctctaa agcggctcgc caagcaggtt ttcgcctgtt
    12541 atgccggaga gcttgacgga caaaagatcg ccaggacggg cttttgcatt cagatgcaca
    12601 ggcgaaaagt tttgagttct gccaaaactt cctttttcaa caataatttg cgcttcttta
    12661 ttaaggtttc gcctaagtaa tttttggagc tgcagttctc ctagcgctcg caagcgcgcg
    12721 gcgcgctctt tgcgctcttt tggtggaacc tgaccgggaa tacgtgcggc gggagtgcct
    12781 ttgcgctctg agtaaggaaa gacatggaga taggtcaagt cgcattcttc gacgatttta
    12841 agggtgtttt ggaacatctc ttcggtttca gtgggaaagc cggtgataat gtcggcaccg
    12901 aattcaatat cggggcggag ggtacgagcc cgctggcaca tcgcaataac atcattgcgc
    12961 aaatggcggc gcttcatgcg tttaaggatc atatcatctc ccgcttgcaa gcttagatgc
    13021 agatgcggca tcaggcgtgg ttcttcggca atcaggcgcc aaaggtcgtc atcgatttcg
    13081 accggatcga gcgatgaaag gcgcagacgc ggcagttcag ggacaagagc cagtacgcgg
    13141 cggatcatct gccctagtgt cggcgcgcca ggcaaatcgg gcccgtatga ggtaacatcg
    13201 acgccggtaa atacaatttc attataacct tgttcgacca gcgcacgaac atgttcggca
    13261 atcaccccta tagggacaga acgcgaaggg ccgcgaccat agggaataat gcaaaaagtg
    13321 cagcggtggt cgcagccatt ttgcacctgc aggaaggcgc gggagcggtc ctcaaagcct
    13381 tcgatcagat ggctggcggt ttcggttaca gacataatgt cgttgaccat gaccttttcg
    13441 ccctgctcca ccccccagct ttcggcttta agctttaaat cattgccgat gatctggtcg
    13501 acctcaggca tagcggcgta ggtatcaggg gtgatctgcg ccgagcagcc cgtgacaatg
    13561 atttttgcat tcggattttc acgccgggca cggcgaatgg cttgccgggc ctgacgctcg
    13621 gcttcttttg ttacagcgca ggtgttaaag atgatggcgt catccagccc cgcattagcg
    13681 gcgtgcgtgc gcatcacctc tgactcatag gtgttgaggc ggcagccgaa ggtgacgagt
    13741 tttggactct tttgatccat gagcgcggtt ttacagattt gttaacctac cgtcaaatat
    13801 ataggcgaca ggaccggtca tgaggacatg gttgtcgctt tcgcgccatt cgatatgaat
    13861 ggggccgcca ttgagaagaa tttcaacttt gcggccatca atgaggccac ggcgtatcgt
    13921 cgcaacagcc actgcgcacg cgccggagcc gcaagccagc gtttcaccgc agccgcgctc
    13981 ccaagtacgc tggcgcagtc ggccatcttc aagaagctga acaaactcga catttgttcg
    14041 ctccggaaac agagcatggt tttctattac agggccgaaa cgttcaacca gcgcattttc
    14101 agcgtcatcg acgaacagta cgcaatgcgg gttgcccatt cctacggcga ctgccgggtt
    14161 atgggtatct gtgcccagct cgaccatcag cgtatcacgt tcctcagcca gaggaatagc
    14221 ggcccaatcg agctgcggcg cgcccatatc gacggtcacc agcccatttt cgacggcgct
    14281 gcagtccagc acgccgcgtc cggtttcgat tgcgcattga tcag
//
LOCUS       DAOSWR010000046        14248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2697_length_14248_cov_5.300500, whole genome shotgun sequence.
ACCESSION   DAOSWR010000046 DAOSWR010000000
VERSION     DAOSWR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_14248_cov_5.300500"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PK513_04900"
     CDS             <1..1443
                     /locus_tag="PK513_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81819.1"
                     /translation="QELKDSETKIVELQGQVESNDKASMKIEELETRQDEMKAALADL
                     VKLSEKYRDSIVQQKEHIEVAERDVAALTAEKTELEIALAAAEQKLENMEPLLREVQS
                     LPDDTEKNVVQESLIAEGKAQPVQAVPEEAGVSSGPVVQSVHIDVPVPAQKPVVKATQ
                     RAEIIGELDVVPVSEPNEILQNGGVALALEQVETEMRITDPNNQARMQVLVREYEALK
                     AFAERQGVEVIRSEIAIAEDSGDKNVYSDLGDVNEAQRQEYMMKMRAEGAHNETEWLV
                     KDMDSQVALEPEDIVMSQSSDPFEDMEVDHASEIELAAQGVDPHVGLEFAPGLYAGVR
                     SSALSITDLITQAKVSSMDKIEKINEGAADAVAYQWSDDSIYGSAEQKTLSSSSQFDE
                     MVQAYLERTQSRCPGEFAVVPDNTIGGDNMRVDSYEVACVGADVDSGASLLFFNQGER
                     FTVVAHEAPTEALGKAMGMRNKVMRFIIGS"
     gene            complement(1610..2098)
                     /gene="phaR"
                     /locus_tag="PK513_04905"
                     /pseudo
     CDS             complement(1610..2098)
                     /gene="phaR"
                     /locus_tag="PK513_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
     gene            2285..3472
                     /locus_tag="PK513_04910"
     CDS             2285..3472
                     /locus_tag="PK513_04910"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOO81820.1"
                     /translation="MSEGVVVIVAAKRTAIGAFGGALSGVSAARLGQVVIEAVITDAG
                     LKPGDIDEVILGQVLTAGLGQNPARQAAIYAGIPEEKTAYTINQVCGSGLRSVALGMQ
                     SILCGDANIVIAGGQENMSASPHCIHMRDGVKFGNTELSDTMIVDGLWDIYNDYHMGI
                     TAENIAEKYSISREEQDTFAAQSQQRAEAAIVAGKFKDEIVPVTIKKRKEEVVFEQDE
                     FPRAGVTVEGLSGLRPAFKKDGSVTAGNASGINDGAAVVVLMRQSEAEKRGLVPLAKI
                     KSWAMAGVDPQVMGTGPIPASAKALKKAGWSVGDLELVESNEAFAAQACCVMKEAGFD
                     PEITNVNGGAIALGHPIGASGARVLTSLLHEMKRREAGKGLATLCIGGGMGIAMCVEK
                     VSR"
     gene            3494..4219
                     /gene="phbB"
                     /locus_tag="PK513_04915"
     CDS             3494..4219
                     /gene="phbB"
                     /locus_tag="PK513_04915"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOO81821.1"
                     /translation="MTNKVAIVTGGTRGIGLEISRTLMADGYKVAAFYAGNEPAAKAF
                     EKETKGRAFKVDVADLGKCEKAVARVEKELGPIGVLVNNAGITRDGMLHKMSDENWHV
                     VIETNLTSCFNMCRAVVGGMRDRGAGRIVNISSINGQKGQLGQANYSAAKAGMLGFTK
                     ALALESARKGITVNAICPGYVKTEMTEAMDSAVLESIVKQIPVGRMGTPKEIAAIVSF
                     LVSERAAFITGATLSANGGQYMV"
     gene            complement(4237..4497)
                     /locus_tag="PK513_04920"
     CDS             complement(4237..4497)
                     /locus_tag="PK513_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81822.1"
                     /translation="MKQRTEQLTEILRAATAPGVMGLNNELANTLNALDDTNMYTEPD
                     ADDGPDSDIKLTSKDCAETIDEMRELSKQTTQQVLDMIRTCG"
     gene            4625..5155
                     /locus_tag="PK513_04925"
     CDS             4625..5155
                     /locus_tag="PK513_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOO81823.1"
                     /translation="MSRGSTVILERRFVPKGTLIMEEGEPGNCAYLIQSGVVEVYTEN
                     EGREVSLAKLDLGQIFGEMALIFDELRTASVRALEDCNLIVITRQSFQAKLGKTDPTI
                     AAIVKMLTQRVISANNTLVNKKSDVKDLVETTEIIYDNILSSLPRARQRTFQASVLPK
                     LEEFLKAVDVFEERYS"
     gene            5256..8708
                     /locus_tag="PK513_04930"
     CDS             5256..8708
                     /locus_tag="PK513_04930"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOO81824.1"
                     /translation="MKWLFAVFSLFVMLLIGLAVAPSFFDWNKYKEPILQQVAELTGH
                     NVRIEGDISLALLPSPRVFLADVMVLDPSDKAGEKVFAAFKQLDVRAQLMPLFNGQVV
                     VRSVRLEAPQVSLFKDVDGRFNFMTPKLEAMVNAKKALSAGEKVSSGAQKFAVSFEDI
                     SIENGVFEYREAGKKAEHKLRGVNLDVQASSLQGPFKAEGSLLYNDQPIEFEAKSGKI
                     DTVLQSTSLNVKARSRGVDARYAGVLSFGGMPEAQGEISVRIEDLKALSGKEASSTGV
                     SGALAVSGLLSANADKASLKNAELEVAGKQLNGAVDIAMKPLSMNGAFTGQDIVDLDS
                     IFGRKKASNGGSFDAASLAGMLPGTLEIQEVGDIKLSLQVPGVVLNGQVLKKVQFTVQ
                     NTEKSFAGLVDVGEVPGQGRMQFSGALSFADKSKSAKTGMVIYSGPVAAFEIKGETQN
                     LPITVQAFTGMNNLPLVRDSKRGVFEFAGKVRPSGLSLDKGIINMDEAAFSLSGAFEG
                     QKDTPRSLLKAKVVADKLDFDTLMNGSGGSVAGDPLAPLKTLALPFDVDLDLRIHDTT
                     IQGHAIKGFGVEAALRPSVLRIVKAGADQFVGSVVNVNGTIGDLKNMAGLDIRVLVDT
                     PDPYKLAGAFKVDTAAWPKDLGRTQASLKASGDLKALNVDASIKALGGEVFVKGRASH
                     PLTQLSVSGLALLVKHPNMDKALQSFAPGAPRYASLSKPMDFAADVAMDGKVTSLSNI
                     KADLAGTTITGAIRLDSSGTKPELNGSLRLGDLVLKSGKSGSSVGQSSAVSGSKSAEK
                     WSSAPMDAGWLHAMNADIDVAANSILYETWDMKKPALKLTLQNGVMNIADLQAGLYDG
                     QISAAGSVSSTGPKAPMIVKMASKIDNINMGALAKALVGSGRIQARGDVSFDFDVSGT
                     GESQAALVGSLNGRANLAGNNVVMKGFDLAGLAGALMESNKPLPRVQQILNASTSSGE
                     TAFDTVKGVYAIEAGRVNITEMVMAGSEAKIDSKGSVNLPRWWIDTMHTVTLANAPDV
                     DPFDVVIKGSLDNPSNTFGSGLFDTFVRQRLQQKVVEELPDLLGKDVTEKLQKFGVLP
                     QKRAKTDGTLPPVTETEPASGEQQPSTQKQPQVQPVAPAPPPKSDEEKAQEAIQGLLK
                     GLLQ"
     gene            complement(8689..8910)
                     /locus_tag="PK513_04935"
     CDS             complement(8689..8910)
                     /locus_tag="PK513_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOO81825.1"
                     /translation="MKKHSVNILGHQTSITLEDEFWDALKTEASAQDLSLNKLVAEID
                     EQRGENNLSSAIRLYILKMLQQKLTEATP"
     gene            complement(8945..9304)
                     /locus_tag="PK513_04940"
     CDS             complement(8945..9304)
                     /locus_tag="PK513_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81826.1"
                     /translation="MYDIPNDFNELLSGCRGEINLVGFGPYDIQIHCGNYTIQTYAHL
                     KYVTKEKTFTGNAEEQQSREVLLKCIGQDIKDIQKINENSFVVSISNGDKITFIADKP
                     EFECIMVRMPDNMDIII"
     gene            complement(9309..10724)
                     /gene="fumC"
                     /locus_tag="PK513_04945"
     CDS             complement(9309..10724)
                     /gene="fumC"
                     /locus_tag="PK513_04945"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOO81827.1"
                     /translation="MSKPAKNLTDTRTESDSIGDMQVPAEAYYGAQTARSLMNFDIGT
                     EKMPSALIRALGIQKKAAALANIKQGSMDKDLGKTIIKAAEEVIDGTLADQFPLAIWQ
                     TGSGTQSNMNANEVISGRANEILTGTRGGKEPVHPNDHCNMGQSSNDTFPTVMHIAAV
                     EEIHTSLLPALEQLHKALEAKSKAFATLVKNGRTHLQDATPLTLGQEFSGYAKQVEYG
                     IARVKDTLPRLYQLAQGGTAVGTGINTRIGFDIEFAAQVAEITGLPFVTAENKFEALA
                     AHDALVETSGALNTLAVSLMKIANDIRLLGSGPRSGIGEIALPANEPGSSIMPGKVNP
                     TQCEALTMVCAQVMGNHTTVSIAGSNGHFELNVFKPVIIYNVLQSIRLLADSTKSFTK
                     RCVEGIEANEKRITELMENSLMLVTALNPHIGYDNAAKIAKKAHADGTTLKQTAIELG
                     LLTEEQFDEWVDPLSMIKPRD"
     gene            complement(10729..11787)
                     /locus_tag="PK513_04950"
     CDS             complement(10729..11787)
                     /locus_tag="PK513_04950"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOO81828.1"
                     /translation="MNKITLGILGGGQLGRMSALAAANLGISVVIFTSEKNSPASLVA
                     AKTIIADYTDERALKEFSKIVDVISYEFENIPIETIHFLQKLKPVYPNEKLLEVSQDR
                     RSEKNFLGSIKIPTGRYAPVDKPQDMLDTLEKWNADSCILKTTRFGYDGKGQARYTSD
                     SNIDTLWTQFNGAPLIMEEIIDFDCEISVIVARDATGKTIHYDPGLNEHKNHILSKTT
                     VPAPLDKNVLSEAVSLAEKLAKDVNLRGVLTLELFVTKDNRLLANEIAPRTHNSGHWT
                     IDACAHSQFENHVRAVCGLPVIEPHRHSPCEMHNLIGDDINLIEKYQRQENACLHDYG
                     KQDIRPGRKMGHITILKD"
     gene            complement(11801..12298)
                     /gene="purE"
                     /locus_tag="PK513_04955"
     CDS             complement(11801..12298)
                     /gene="purE"
                     /locus_tag="PK513_04955"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOO81829.1"
                     /translation="MTHTDKDPQVGIIMGSQSDWDTMGEAHDILHELNIPHEVKIVSA
                     HRTPERLYNYAETAKNRGLKVIIAGAGGAAHLPGMTASMTPLPVLGVPVESKALKGMD
                     SLLSIAQMPGGIPVGTLAIGKAGAKNAGLLAAAILATADKTIAKALDDFRAKQTADVA
                     QTPSD"
     gene            complement(12326..12547)
                     /locus_tag="PK513_04960"
     CDS             complement(12326..12547)
                     /locus_tag="PK513_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOO81830.1"
                     /translation="MYDFSDMEDQEELQAKLAEYQSEHKALDHMIEIAMASDKPVNLL
                     HIQQLKKKKLWLKDMIKKIESDLIDDIIA"
     gene            12684..13766
                     /locus_tag="PK513_04965"
     CDS             12684..13766
                     /locus_tag="PK513_04965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOO81831.1"
                     /translation="MTMSKTIGILTSGGDCAGLNAVMRAVVHRADLLGWSVIGIENGT
                     AGLLETPYRVRKLHPTDFDGFVMRRGGTILGTTNSRDPFRYKVDDGTVKDRSEEVIAA
                     LKDLDIEALIGIGGDGSFDILSRLTKKGGLNMVGIPKTIDNDLAMTDVSVGFDTALEV
                     ATEALDRLQPTAASHDRVMILEVMGRDAGHIALNAGIAGGADVILIPEIPYTLEGIAK
                     KIQRVKESGRNFALVIVSEAVKGPEGEKREVVYHGGETRYGGIGEYLGPRIAEATGFE
                     TRVTVLGHVQRGGNPTHNDRLLAQGLGVKAVDLIAEGTYGKMVAWRGREAVAVPIEDA
                     IKKCRVVESDGFLVHTARSLGISFGD"
     gene            13833..>14248
                     /locus_tag="PK513_04970"
     CDS             13833..>14248
                     /locus_tag="PK513_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO81832.1"
                     /translation="MLYVEQSLGPNEELVHVGKFHWMYTVHAFMAIIWGIIISLMIVV
                     GGVIVYKQMGIFPANLPIMEGLQNLDLLHPGLRIAAFIAFVLGLLSFAQMMVVKATTE
                     IAITNTRLVYKRGLVARQVGEMSIDRIEGVNVLQTI"
BASE COUNT         3217 a         2659 c         4313 g         4059 t
ORIGIN      
        1 caggagctca aagattctga gacaaaaatt gttgagttgc agggccaggt tgaaagtaat
       61 gataaagcct ctatgaaaat tgaggagctt gaaacacggc aagatgaaat gaaggctgcg
      121 ttggcagatc ttgtgaaact ttcagagaaa tatcgcgata gtatcgtgca acaaaaagag
      181 catattgagg ttgctgagcg ggatgttgct gcattgacgg cagagaaaac agagcttgag
      241 attgcgttag ctgctgcaga gcagaagctt gaaaatatgg aacctctttt acgtgaggtg
      301 caatctttac cggatgatac tgaaaagaat gttgttcagg agagtctgat tgcggaaggg
      361 aaagctcagc ccgttcaggc tgtgccggag gaggctgggg taagttctgg tcctgttgtc
      421 cagagtgtgc acattgatgt gccggttccg gcacaaaaac cagttgttaa ggcgacacaa
      481 agagctgaaa ttattggaga gttagatgtt gtacctgttt ctgagcctaa tgaaatactt
      541 caaaatggcg gcgttgcctt ggctttagaa caggttgaaa cggaaatgcg tataacggat
      601 ccgaataatc aggcgcgaat gcaggtcttg gtgcgggagt atgaggcgct taaggctttt
      661 gctgagaggc agggtgttga agttattcgt tcagaaatcg ctatagcgga ggatagtggt
      721 gataagaatg tttactcaga tttaggtgat gtgaatgaag cgcagcgtca ggaatatatg
      781 atgaaaatgc gcgctgaggg cgcgcataat gagaccgagt ggctggtaaa ggatatggac
      841 tcgcaggtgg cgcttgagcc ggaggatatt gtgatgagcc aaagttccga tccgtttgag
      901 gatatggagg ttgatcatgc gagtgagatt gagcttgctg cacagggcgt cgatcctcat
      961 gttgggctgg aatttgcgcc tgggctatat gcaggggtgc gttcgagtgc gttgtctatt
     1021 actgatttga ttacgcaagc taaagtttct agcatggata agattgaaaa aattaatgaa
     1081 ggggcggcgg atgctgtggc ctatcagtgg agcgatgata gtatttacgg ttctgctgaa
     1141 cagaaaacgc tttcttcgtc ttcgcagttc gatgagatgg ttcaggctta tcttgagcgt
     1201 acgcagagcc gttgtcctgg tgagtttgct gtcgttcccg ataatacgat tggtggtgat
     1261 aatatgcgcg tcgatagtta tgaagttgca tgtgtcgggg ctgatgttga ttccggggcg
     1321 tcattgctct tctttaatca aggggagagg tttaccgttg ttgcacatga ggcaccgaca
     1381 gaggcgcttg gcaaagctat ggggatgcgc aataaggtga tgcgttttat tatcggttca
     1441 taatttttat gcggtccgta ggacagaaca ccacgggttt acccgtgggt atctattgac
     1501 aaacgggctc gccaattttt tgggagcccg ttttttttcg catgcacgaa actgagtttc
     1561 taaattattt ttgctttttt tgaggatctt gttccgtgta atccgcatta aaaggcatga
     1621 acattttcat ggtgttttca aacatttcca tgtttttgcg gctcatttct tcaaagtcgg
     1681 cacttccggg gataggcgga aacatatttt gtatcgggtt tatgccttca aatgatttgt
     1741 tgatttgttc gcgtacctgt tcctggtttt taacaaaagt atcgagcgtt tgttcgagat
     1801 aattgggcac gattgactgg atgttgtcgc cgtaaaagcc tatgaggttt ttgagaaagc
     1861 cggtgggaag aagattctcg cctttggatt cctgttcgac aataatttgt gtgagtacgg
     1921 agcgggtgat atcggtgccg ctcttggcat catagacgac gaaatcaatg ccgtctttaa
     1981 ccatttcaca taaatcatca agcgtgacgt agctgctgcg tcctgtatca tataggcggc
     2041 ggttagcata tttcttaatt acagtcgggt tatcctgccc gttcttacct ttggccatgt
     2101 tgcaccttct ttggttaaaa gttcataatt tgctgcagtg gaatagggta taataagcat
     2161 ataatgtttt ttttgaaaag aaaattttgc gttgcatata attattttta tttacatgtg
     2221 gtgtggcgcg ccatatcttg gcggtgattt aaagtgtttt ttgttctgga aatggagagt
     2281 gtaaatgagt gaaggtgtgg tggtaattgt tgcagcaaag cgtacggcta ttggtgcgtt
     2341 tggcggcgcg ttgtcaggtg tgtctgctgc aagactgggg caggttgtga tagaggctgt
     2401 catcacagat gcggggctta agcccggtga tattgatgag gttatattag ggcaggtttt
     2461 gacggctggg cttgggcaaa atccggcgcg gcaagctgct atatacgcag gtattcctga
     2521 ggagaaaacc gcttatacaa ttaatcaggt ttgtggttcg ggtttgcgta gtgttgcgct
     2581 gggaatgcag tcaattttgt gcggtgacgc gaatattgtt attgctggtg gtcaggaaaa
     2641 tatgagtgct tcgccacatt gcatacatat gcgtgatgga gtcaaatttg gaaatacaga
     2701 acttagtgac acgatgattg tggacgggct ttgggacatc tataatgatt accatatggg
     2761 aattacggca gaaaatattg cggaaaaata ttccatttcc cgtgaagagc aggatacgtt
     2821 tgcagcgcaa tctcaacagc gtgcggaagc cgcgattgtt gcaggtaaat ttaaagatga
     2881 aatcgtgccg gtgacgatta aaaagcgtaa agaagaggtt gtgtttgaac aagatgagtt
     2941 tccgcgtgcg ggtgtgacag tagaggggct tagcgggctt cgtccggcat tcaagaaaga
     3001 cgggagcgtg acggcaggaa atgcttcggg tattaatgac ggagcggcag ttgttgtttt
     3061 gatgcgccaa agtgaggcgg aaaagcgagg gcttgtgcca ttggcgaaga ttaaaagctg
     3121 ggctatggcg ggagttgatc cgcaggtgat gggtacgggg ccgattccag ccagtgcgaa
     3181 ggctttgaaa aaagcggggt ggtctgtcgg tgatctggaa ttggtggaat caaatgaggc
     3241 ctttgcggcg caggcttgct gcgttatgaa agaagccggt tttgaccctg agattaccaa
     3301 tgtgaatggc ggggcgattg ctctggggca tccgattggc gcgtcgggtg cgcgggtgct
     3361 tacgagctta ttgcatgaaa tgaagcggcg cgaggccggg aaggggctgg caacgctttg
     3421 tattggaggc gggatgggga ttgccatgtg cgttgaaaaa gttagtcgtt agtagttttt
     3481 agaaggaaga aaaatgacaa ataaagttgc aattgttacg ggcggaacgc gcgggattgg
     3541 tctggagatt tcgcgaacgc tgatggctga tgggtacaag gttgcagcct tttatgcggg
     3601 caatgaacct gcggccaagg cctttgaaaa agaaacaaaa ggccgggcgt tcaaggttga
     3661 tgtggccgat cttggtaaat gcgaaaaagc tgtggccagg gttgagaagg aactggggcc
     3721 gatcggtgtt ctggtcaata acgctggcat tacgcgggac ggaatgttgc ataaaatgag
     3781 tgatgagaac tggcatgtgg tgattgagac aaacctgacc tcctgcttta atatgtgccg
     3841 ggctgtggtt ggcgggatgc gcgatcgggg cgcgggacgg atcgttaata tcagttcgat
     3901 caacgggcaa aagggccaac tggggcaggc gaattattca gcggccaagg ccgggatgct
     3961 tggttttacc aaggcattgg cgttggaaag tgcacgtaaa ggtattacgg tgaatgcgat
     4021 ttgtccgggc tatgtcaaga cggagatgac cgaggcgatg gattctgccg ttttagaaag
     4081 tattgtgaag cagataccgg tggggcgaat ggggacgccg aaggaaatcg cggcgattgt
     4141 gtcgttttta gtgtctgagc gggctgcgtt tattaccggt gcaacacttt cggctaatgg
     4201 tggtcaatat atggtctagg tcatcaggaa catggcttag ccgcaagttc gtatcatatc
     4261 cagaacttgc tgggttgttt gcttcgatag ttctctcatt tcgtctatag tttctgcgca
     4321 gtcttttgat gttagtttta tgtctgaatc aggcccatca tctgcatcag gttcggtgta
     4381 catgttcgtg tcgtcaagtg cgttgagcgt gtttgcgagt tcgttgttta gtcccattac
     4441 gcctggagcg gttgctgctc tcagaatttc ggtgagttgt tctgttcttt gcttcatctt
     4501 tctagctctt aaattgttgt tttctttaat tgtttttatc atgtgtcttt gtagctggca
     4561 aactcatagt ttgttcgatt ggattcctct ttctttacag gtttatgctg ggtataacgg
     4621 atatatgagt cgcggatcta cagtcatact tgaacgccgg tttgtcccta agggaacctt
     4681 gattatggaa gaaggggagc ctgggaattg cgcgtatctt attcaatcgg gcgtggttga
     4741 ggtttatacc gagaatgaag gtcgggaagt tagtctggcc aaactagatt tggggcagat
     4801 tttcggggaa atggcgctga tctttgatga gttgcgcacg gcgtctgtgc gggcgcttga
     4861 ggattgtaat ttgattgtta ttacgcgcca gtcctttcag gcgaagttgg gaaaaacgga
     4921 tcctactatt gcggcgattg tgaaaatgct tactcagcgt gtgatttcag caaataatac
     4981 actggttaac aagaagagtg atgttaagga tttagtggag acgacggaga ttatttatga
     5041 caacattctc tcgtctttgc cgcgtgctcg gcagcgaaca tttcaggctt ctgttttgcc
     5101 aaagctggaa gagtttttaa aggctgtcga tgtttttgaa gagcgctatt cctagctctt
     5161 gttttcttgt catttgcagc atttttccta tataaaagca tgaaccttta aacttttgtg
     5221 tggcttgttt atgctctcgc gtcaggagat ttcttatgaa atggttattc gctgtttttt
     5281 cgttgttcgt tatgctgttg atcggtttgg cggtggcacc gagctttttt gattggaata
     5341 aatacaaaga gccgattttg caacaggtgg cggagcttac agggcataat gtgcgtattg
     5401 aaggtgatat ttcgctggcg ctgttgccta gtccgcgggt gtttttggct gatgtgatgg
     5461 tgcttgatcc gtcggataag gcgggggaaa aagtgtttgc agcgtttaag cagcttgatg
     5521 tgcgcgccca attgatgccg ctcttcaatg ggcaggtagt cgtgcgttcc gtgcgtctgg
     5581 aggctccgca ggtttcgctg tttaaggatg tggatgggcg gtttaatttt atgacgccga
     5641 agttggaagc tatggtgaac gcgaaaaagg cgctgagcgc tggcgaaaag gtgtcttctg
     5701 gggcacagaa atttgcagtt tcgtttgaag atataagcat tgaaaacggt gtttttgagt
     5761 atcgcgaggc tgggaaaaag gcagagcata agcttcgggg ggtgaatttg gatgtgcagg
     5821 caagctcttt gcaaggtccg tttaaggcgg aaggatcgct cctttataat gatcagccga
     5881 ttgaatttga ggctaagagc gggaaaattg atacggtatt acaatcgacc agtttgaatg
     5941 taaaagcgcg ttctcgcggc gttgatgcgc gttatgcggg cgtgttgagc ttcggtggta
     6001 tgccggaggc gcaaggcgag atttctgttc gcattgaaga tttaaaggcg ctgagtggca
     6061 aggaggcatc gtcaacggga gtatcaggcg ctttggcggt tagcggtttg ttgagcgcga
     6121 atgctgataa ggcttcactt aaaaatgctg agttagaggt tgcgggaaag cagcttaacg
     6181 gggctgttga tattgctatg aagccgctga gcatgaacgg ggcgtttacc gggcaagata
     6241 ttgttgatct tgactcgatt ttcgggcgca aaaaagcttc aaatggcggg tcttttgatg
     6301 cggcttcttt ggccggaatg ctgccgggaa cacttgagat tcaagaggtt ggcgatatta
     6361 aattgtcttt gcaggtgccg ggagtggtcc tgaacgggca agtcttaaaa aaggttcaat
     6421 ttactgtaca aaatacagag aaaagttttg ctggtctcgt tgatgtgggg gaagtacctg
     6481 gtcaggggcg gatgcagttt tccggggcct tgagttttgc ggataaatct aagtcagcca
     6541 agacgggtat ggtgatttat tccggtccgg tggcagcgtt tgaaattaaa ggtgagacac
     6601 aaaacttacc cattacggtg caggctttta ccgggatgaa taatttgccg ttggtgcgag
     6661 attccaagcg cggagtgttt gaatttgcag ggaaggtgcg tccgtctggc ctgtcgcttg
     6721 ataaaggcat aatcaatatg gatgaggcgg cgttttcact ttccggtgca tttgaggggc
     6781 aaaaagatac gccgcgctct ttattgaaag ctaaggtggt tgcagataaa ctggattttg
     6841 acactctgat gaacggttca ggtgggtcgg tggctgggga tcctttagcg ccgctgaaaa
     6901 cgctggcgct tccctttgat gttgatttgg atctgcggat tcatgatacg accattcagg
     6961 gacatgctat taaaggtttt ggcgtagagg cggcgctgcg gcctagtgtt ttacgtattg
     7021 tcaaagcggg ggcggatcaa tttgtcggat cggttgtgaa tgtgaacggg acgattggcg
     7081 atttgaaaaa tatggcgggg ctggatattc gcgttttggt cgatacgcct gatccttata
     7141 agctagccgg ggcatttaag gttgatacgg cggcgtggcc gaaggatctg gggcgaacgc
     7201 aggcgagcct taaggccagc ggggatttga aggcgttaaa tgttgatgcg tctattaagg
     7261 cgctcggcgg tgaggttttc gtgaaggggc gggcttctca tccgttgact caactttctg
     7321 ttagcggttt ggcgctattg gtaaagcatc cgaatatgga taaggcgttg caaagctttg
     7381 cgccaggcgc gccgcgttat gcctctttga gcaagcctat ggattttgca gcggatgtgg
     7441 cgatggatgg caaggtgaca tctttgagca atatcaaggc tgacttggct ggcacaacga
     7501 taaccggagc aattcgactg gattcgagcg gtactaagcc tgagctgaat ggaagtctgc
     7561 ggcttggcga tctggtgttg aaaagtggca agagtggttc atctgtgggt caatcttccg
     7621 ccgtttccgg ttcaaaatct gctgaaaaat ggtcgagcgc gcctatggat gcgggctggt
     7681 tgcatgcgat gaatgcggat atcgatgtgg cggcgaattc tatactttat gaaacgtggg
     7741 atatgaagaa accggctttg aagcttacac ttcaaaatgg tgtgatgaat attgctgatt
     7801 tacaggcagg cttgtatgac gggcagatta gtgcagcggg cagtgtttca tcaacgggac
     7861 cgaaggcacc gatgattgtg aagatggcta gtaaaataga caatattaat atgggggctt
     7921 tggccaaggc tcttgtcggc tccgggcgca ttcaggcgag aggggatgtg tcatttgatt
     7981 ttgatgtatc cggcaccggg gaatcgcagg cggcgcttgt cggatcttta aatgggcggg
     8041 ccaatctggc cgggaataat gtggtgatga aggggtttga tctggccggg ttggcaggtg
     8101 ccttgatgga aagcaacaag ccgttgccgc gggtgcagca gattttgaat gcgtcgacca
     8161 gcagtggaga gactgcgttt gatacggtga agggcgttta tgcgattgaa gcgggccgcg
     8221 ttaatattac cgagatggtg atggcggggt ctgaggcaaa gattgactcg aaagggtctg
     8281 tgaatttgcc gcgttggtgg attgatacga tgcacacggt aacgctggcc aatgcaccgg
     8341 atgtggaccc gtttgatgtg gtgattaagg gatctctgga taatcctagt aatacgtttg
     8401 gtagcgggtt gttcgatacg tttgttcgtc aacgcttgca gcaaaaggta gtggaagagt
     8461 tgccggattt actgggtaag gatgtgacgg agaagttgca gaagtttggt gttttgccgc
     8521 agaaacgtgc taaaacagat ggaacactgc ctcctgtaac agaaactgag ccggcttctg
     8581 gtgagcagca gccttctacg cagaagcagc cgcaagtgca gcctgtagcg cctgcgccgc
     8641 cgcctaagag tgatgaagaa aaagcacaag aagccattca ggggttgctt aaggggttgc
     8701 ttcagtaagt ttttgttgca gcattttgag gatatagagg cggatagcac tggagagatt
     8761 attctcgccg cgctgctcgt cgatttcagc gacgagttta ttgagtgaga ggtcctgcgc
     8821 gctggcttcg gtttttagtg cgtcccagaa ctcgtcttcc agtgtgatgc tggtttgatg
     8881 gccaaggatg ttgacggagt gttttttcat ttttaccaca gaggcacgga gtacacagag
     8941 ttttttaaat aataatatcc atattgtctg gcattcttac cattatgcat tcaaattccg
     9001 gtttatccgc aatgaatgta attttatctc cattagaaat gctcacgaca aagctatttt
     9061 cgtttatctt ttgaatatcc ttaatatctt gcccaatgca ctttaaaaga acttctctac
     9121 tttgttgctc ttctgcatta ccagtgaagg ttttttcttt tgttacgtat ttgaggtgtg
     9181 catatgtttg tatcgtgtag tttccacagt ggatctgaat atcgtatggc ccgaaaccta
     9241 ctagattaat ttccccacgg catccagata ataactcgtt gaagtcgtta ggtatgtcat
     9301 acattttttt aatctcttgg tttgatcata gagagcgggt ctacccattc gtcgaattgc
     9361 tcttctgtga gcaggccgag ttctatggct gtttgtttga gcgttgtgcc gtcggcgtgg
     9421 gctttcttgg cgattttggc cgcgttgtca tagccgatat gagggttgag cgcggtgaca
     9481 agcattaatg agttttccat caactcagtg atgcgttttt cgtttgcctc aatgccttcg
     9541 acgcagcgct tggtaaagct tttcgtactg tcggcgagca ggcggattga ttgcaggaca
     9601 ttgtagatga tgacgggttt gaagacgttg agttcgaaat gtccgttaga gcccgctatc
     9661 gatacagtcg tatgattgcc catgacttgt gcgcagacca tggtgagggc ttcacactgg
     9721 gttgggttga cctttccggg catgatagag gagccgggct cgttggctgg cagggcaatt
     9781 tcgccgatcc ccgatcgcgg gccggagccg agcaagcgga tgtcgttggc gattttcatg
     9841 agcgatacgg ccagcgtgtt gagcgcgccg gaggtttcta ccagtgcgtc gtgggcggcc
     9901 aaggcttcga atttattttc ggcggtgaca aagggcaagc cggtgatttc agcgacttgc
     9961 gcggcgaatt ctatgtcaaa tccgatgcgt gtgttgatgc cggtgccgac agccgttccg
    10021 ccctgggcga gttgataaag gcgcggcagg gtgtctttta cacgggcgat accatattcg
    10081 acctgtttgg cgtagccgga gaattcctgg cctagcgtga gcggtgtggc gtcctggagg
    10141 tgtgtgcggc cgtttttgac gagcgtggcg aaggctttgg atttagcctc cagcgctttg
    10201 tggagttgct ccaaggcggg gagcagcgat gtgtgaattt cctccacggc ggcgatgtgc
    10261 attactgtgg ggaaggtatc gttcgagctt tggcccatat tgcagtgatc gttcgggtga
    10321 acgggttctt taccgccgcg tgtgccagtg aggatttcgt tggcgcgccc ggagataact
    10381 tcgttggcgt tcatgttgga ttgtgtgccg gagccggttt gccatatggc gagagggaac
    10441 tggtcggcca gtgtgccatc aatgacttcc tccgcggctt tgatgatggt tttaccgagg
    10501 tctttgtcca ttgagccttg cttgatattg gccaaggcgg cggctttttt ctgaatgccc
    10561 agagcacgga tgagggcgga aggcattttt tcggtgccga tgtcgaaatt catcaaagaa
    10621 cgagcggttt gcgcgccgta ataggcctcg gccgggactt gcatatcgcc gatagaatcg
    10681 ctttctgtgc gggtatctgt gaggtttttc gctggcttgc tcatgttttt agtcctttag
    10741 aattgtgata tggcccattt tgcggcccgg ccttatatct tgcttgccgt agtcatgaag
    10801 gcaagcgttt tcttgtctct ggtatttttc aataaggttt atgtcgtcgc caatgagatt
    10861 atgcatttcg caaggtgaat ggcggtgagg ctctatgacg ggcaggccgc atacggcgcg
    10921 gacgtggttt tcgaattgtg agtgggcgca ggcgtcaatc gtccaatggc cggaattgtg
    10981 ggtgcgcggg gcgatttcat tggcgagcag gcggttatct ttggtaacaa aaagctccaa
    11041 cgtcaggact ccgcgcaggt ttacgtcttt cgccagtttt tcggcgagcg aaacagcttc
    11101 gctcaggaca tttttatcga gcggggcggg tacggtggtt ttggagagga tatggttttt
    11161 gtgttcgttg agaccgggat cataatggat ggtttttccg gtggcatcgc gggcgacgat
    11221 gacggaaatt tcacaatcga aatcgataat ctcttccatg attaaaggtg cgccattaaa
    11281 ttgagtccat aacgtatcga tattggaatc gcttgtatag cgcgcttgac ccttgccatc
    11341 atatccgaaa cgtgtcgttt ttaatatgca ggagtccgca ttccattttt caagcgtatc
    11401 gagcatatct tgaggtttat ccacaggggc ataccgaccg gtcggtattt ttatgctgcc
    11461 caggaagttt ttttcgctgc gacggtcttg agaaacttcc agtagctttt cattcggata
    11521 aacgggctta agtttttgca ggaaatggat tgtttctatt ggaatatttt cgaattcata
    11581 agaaattaca tcaacaattt tggaaaactc ttttagggcg cgctcgtcag tataatcggc
    11641 aataatcgtc ttcgcggcga cgagagaggc cggagaattt ttctcagaag tgaagatgac
    11701 aacggaaatg ccaagattgg ctgcagccag cgcgctcatt cgcccgagct gtccgccgcc
    11761 gagtatgccg agtgtgattt tattcatgga cgaggttata tcaatctgag ggtgtttgtg
    11821 caacgtcagc tgtttgtttt gcgcggaaat catccagagc tttggcgatg gttttatcgg
    11881 ctgtggccag aatggcggcg gcaagcaggc ctgcgttttt ggcaccggct ttgccgatgg
    11941 caagcgtacc gacagggatg ccgccgggca tttgcgcgat ggaaagcagg gagtccatgc
    12001 ctttgagggc cttgctttca actggaacgc ccaaaacggg cagcggggtc atggatgcgg
    12061 tcatgccggg caggtgcgcc gcgccgcctg cgcctgcgat aatcaccttc agtccacgat
    12121 tcttagcggt ttcggcgtaa ttatacaggc gctccggtgt gcgatgcgcc gaaacaattt
    12181 tgacttcgtg cgggatgttt aattcgtgca ggatgtcatg ggcctccccc atggtgtccc
    12241 aatcgctctg ggagcccatg atgatgccaa cttgagggtc tttatccgta tgagtcatga
    12301 gcactttata aatgttttga ggggattaag caataatgtc gtcgatcagg tcgctttcaa
    12361 tctttttgat catgtctttg agccacagct ttttcttttt gagctgttgt atatgtagta
    12421 aattgacagg cttatcactc gccatcgcga tctcgatcat gtgatcaagc gctttgtgct
    12481 cgctctggta ttccgccagt ttcgcttgaa gttcttcttg atcttccata tcagaaaagt
    12541 cgtacaaaaa ttgatccttt aaaggcttaa gcctaacaca taaaatgttg tttgataata
    12601 gaaaatgcgt atcatggata tagaatttaa cagggtattc attcttcgcc ttgtaaactc
    12661 ttcttttaat tctcttaagg tggataacta tgtcaaaaac aattgggatt ctgaccagtg
    12721 gcggtgattg tgccgggtta aacgcggtta tgcgggcggt ggtgcatcgg gccgatttgc
    12781 tcggttggag cgttatcggc attgaaaatg gtacggcagg gttgttggaa actccttatc
    12841 gtgtacggaa attgcatcca actgattttg acgggtttgt tatgcgccga ggaggaacga
    12901 ttttggggac gacgaatagc cgcgatcctt ttcgttataa ggtggatgat ggaacggtta
    12961 aggatcgctc ggaggaagtg attgccgcgc ttaaggatct ggatattgag gcgttgatcg
    13021 ggattggcgg tgacgggagt tttgatattc ttagccgttt gaccaagaag ggcggtctta
    13081 acatggtggg aatcccgaag acgattgata atgatctggc gatgacggat gtttctgtcg
    13141 gatttgatac ggctttggaa gttgcgacag aggctttgga ccgtttgcag ccaacggcgg
    13201 ccagtcatga ccgggtgatg attttggaag tgatggggcg cgatgcgggg catattgcgt
    13261 tgaatgcggg cattgccggc ggggcggacg tgattttgat tcctgaaatc ccttatacgc
    13321 tggaagggat tgcgaagaaa atacaacgcg ttaaagaaag cgggcggaat tttgcgcttg
    13381 tgattgtgtc ggaagctgtg aaagggcctg agggtgaaaa gcgcgaggtt gtttatcatg
    13441 gcggtgaaac gcgttatggc ggaattggtg aatatttagg gccaagaatt gccgaagcaa
    13501 ccgggtttga aacgcgcgtg actgttctgg ggcatgttca gcgcggcggg aatcctaccc
    13561 ataatgatcg tttactggcg caagggctgg gggttaaagc cgtcgatctt atcgcggaag
    13621 ggacatatgg caagatggtc gcgtggcgtg gccgggaggc tgtggccgtg ccgattgaag
    13681 atgcgattaa aaagtgccgc gtggtggagt ctgatggctt tttggttcat acagcccgca
    13741 gcctcgggat ttcattcggg gattagaaaa gcgggattga agcaggtcag cgtttcaggc
    13801 taagctgatg ggaacaagat taaggaattt gaatgctata tgtagaacaa tcgctggggc
    13861 cgaatgaaga gctggttcat gtcgggaaat ttcactggat gtacacggtg catgccttca
    13921 tggcgattat ctgggggatt atcatcagcc tgatgatcgt ggttggcggt gtgatcgttt
    13981 acaaacaaat gggcatcttt cctgctaatt tgccgattat ggaaggtttg caaaatttag
    14041 atcttttgca tccaggtctt agaattgcgg cctttattgc cttcgtcctt ggtctgctta
    14101 gctttgcgca gatgatggtg gtgaaggcga cgactgaaat tgcgattacc aatacgcggt
    14161 tggtgtataa gcgcgggctg gttgcgcggc aggtcggtga gatgagtatt gaccggattg
    14221 aaggggtgaa tgttttgcag actatttt
//
LOCUS       DAOSWR010000047        13636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2857_length_13636_cov_5.443487, whole genome shotgun sequence.
ACCESSION   DAOSWR010000047 DAOSWR010000000
VERSION     DAOSWR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2857_length_13636_cov_5.443487"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..850)
                     /gene="mnmD"
                     /locus_tag="PK513_04975"
     CDS             complement(137..850)
                     /gene="mnmD"
                     /locus_tag="PK513_04975"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOO81833.1"
                     /translation="MFGASPKGAPRSKEFDDVYFSADDGLAETEYVFLDGNHLPRGWQ
                     GRDRFVIAETGFGTGLNFLAAWKRFEETSTSDQRLDFISFEKFPLDGEEIHLHLHPWD
                     PFFGAHLRKMIRLYPPLMPGFHRLSLSGRVNLTLVFDDVNAAISRIDTKVDAWFLDGF
                     KPSSNPDMWTDVVFQNMARLSYNGSRMATFTAAGRVRRGLEAVGFAVEKVPGFGRKRD
                     MTIGVFKHGDSLSDRGTEI"
     gene            902..2950
                     /locus_tag="PK513_04980"
     CDS             902..2950
                     /locus_tag="PK513_04980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOO81834.1"
                     /translation="MSKNPLLEISNLPNHAPAFGAIKNEHYMPAVKAAIEEARAVYES
                     IKKNPEEPNFKNTVEAMETAGETLSQVTSIFYNQLSSTGGDELHELAEKIGPITSNFS
                     SDIILDAALFARLKAVHDKIEELDLTVEQHTLLEEDYKNFVRGGALLPEDKKQRLREI
                     NEALSTLGPAFMNNANKSAEKFEMVIKDKADLAGLPESAIEAAALSAKEKNMNGQWRF
                     TLDYPSYAPFIQYADNRELREKMWRAFGSRAYGDEFDNCENIKKIITLKDERARLLGY
                     KTHAHYVLERRMAENPSSVLGFLKKLKSTYKPAAKKDLAQLQKFAKKHGLESELKPWD
                     VGYYAEKLKQKLFDFSSEDLRPYFPLDKVLAGCFEHFSQLFNIEFREAKDYETWHKDV
                     KTFELYNKTSGAFIGTLFGDFFPRTGKKNGAWMTAYRSQGLFHGKIERPVIGIVCNFT
                     KPTEDKPSLITHGEVTTLFHEMGHAIHGLLSNITYRSLSGTSVLWDFVELPSQLQENW
                     CYEKKTLDHFAAHYETGEKIPVNLIEKVRKAKNFMVGWGGLRQIGLGMLDMTWHTADP
                     STIDDVAAFEDKALKDTTLFPRLAGPTSTSFSHIFAGGYSAGYYSYKWAEVLDADTFE
                     LFLEKGLYDRKTAKAYQEKILSKGGSQHPAILYRNFRGRDADPDALLRREDLLKDKAA
                     "
     gene            complement(3049..3124)
                     /locus_tag="PK513_04985"
     tRNA            complement(3049..3124)
                     /locus_tag="PK513_04985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3089..3091),aa:Met,seq:cat)
     gene            complement(3414..3839)
                     /locus_tag="PK513_04990"
     CDS             complement(3414..3839)
                     /locus_tag="PK513_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81835.1"
                     /translation="MGDTKYTALGRTNTRIIEQGIMPAEKFQKNKYSPKADYPKDVTD
                     QFNTKALPLDHIEETFLRLYERQKLFADNNTAYTSPQRITATMMEELDDRGPEGRHPA
                     IEAAFQDIMSRPDKFGLEVKEAGMFNFGGPHNFTPIVPQ"
     gene            complement(3973..4431)
                     /locus_tag="PK513_04995"
     CDS             complement(3973..4431)
                     /locus_tag="PK513_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81836.1"
                     /translation="MAEPGDITLASYNSAGVSKDTRNRLAEIYKEIGREPSVGSKIKV
                     EGPKAEFTKCADPVCTFMNQYNDINEALERDGGVISEISSWGINANNFDDVITMAKKG
                     LIPEEISDEVNAAIAEVEANQAKYRIEDGWFGSHDSTAADNRMELPGPSS"
     gene            complement(4555..5721)
                     /gene="mnmA"
                     /locus_tag="PK513_05000"
     CDS             complement(4555..5721)
                     /gene="mnmA"
                     /locus_tag="PK513_05000"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOO81837.1"
                     /translation="MNSLGINKAPKDTRVVVAMSGGVDSSVAAALLYEQGYDVVGVTL
                     QLYDSNDHGGEAVAKSKTCCAGQDIYDAKKVAGICGFPHYVLNYESNFRESVIEDFAD
                     SYMRGQTPIPCVKCNQSVKFHDLLQVAKDLGADCMATGHYIKRVINDDGRATLHRAHD
                     HGKDQSYFLFATTQEQLDFLRFPLGGWSKDKTRREADRLGLITADKPDSQDICFVPHG
                     DYARVVKKIRPEAEQPGDIVHIDGRTVGTHEGIIHYTIGQRKGLGIGGGVSENNEPLY
                     VVQIDPATNTVIVGPKEALARDILYLEDCNWLYDTKNLKAKKGCAFLPVTVKFRSVMQ
                     PTPAVLNTEKNEIRLDQPQYGISPGQAAVCYAGDRVLGGGWITKTSKKSAQNAA"
     gene            5812..6537
                     /locus_tag="PK513_05005"
     CDS             5812..6537
                     /locus_tag="PK513_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81838.1"
                     /translation="MFSYIRRQILQKRLGFAFKQKELPIIDQNPEFFFSGDYLRHARE
                     TKERIEKIILEKGFEGNFDLVLVDEFNHCARKIKPIYGRETTVLQDFMASQKDSYQTL
                     RDFFTEAKDVLVYEASSLRDFAYSQLTVASKKALREELGKISSSDFFEATPLFQAFHF
                     WKAAMESNYLNKDRGNCDFWGDSLRVSCMRGEGDLMVRFYESVYNLDVYGNEPPEGHR
                     RRDEELLKGVLGGFDWDAAVPSH"
     gene            complement(6541..6828)
                     /locus_tag="PK513_05010"
     CDS             complement(6541..6828)
                     /locus_tag="PK513_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOO81839.1"
                     /translation="MIRHIVMFSAKNKDDLDTIESGLMNLTQIPGAKIEITRNQKADQ
                     LGNDIDIVVYGEFPDLETLNAYKRHPLYEKSTSIVRPLRELRFAADIEAAT"
     gene            complement(6825..7169)
                     /locus_tag="PK513_05015"
     CDS             complement(6825..7169)
                     /locus_tag="PK513_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO81840.1"
                     /translation="MPKITFLHKDGSHQIIEAENNWSIMQVAVEHKIKGIEGACGGSM
                     ACATCHVYIHSDWIDRVTAAGNEQCEEEEDMLDMAFDVRDTSRLGCQVKLTEDLDGLV
                     VTLPGTKEEAFS"
     gene            complement(7162..8271)
                     /locus_tag="PK513_05020"
     CDS             complement(7162..8271)
                     /locus_tag="PK513_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOO81841.1"
                     /translation="MDKKTYLDYNATAPTRPEVIAIINECLSAPHNASAVHSFGREGR
                     KIVECAREKIAALVGVPPAQIIFNSGATEGNNTVLRHFTKERVLVSAIEHPSVLETVP
                     KSDRIPTTPEGVLDLETFEILLKAEKTALVSVMLANNETGIIQPVKEAAALAHKHGAL
                     FHCDAVQAAGRIPIDMAALGIDFFTLSAHKIGGPQGIGALALGICGITPTLLHGGGQE
                     KSARAGTENVAGIAGFGIAAELAQDGIQDYQNKTRALQTKLETTLKNIKIYGKNAPRV
                     ANTTLFSQPGVSSETLLIALDLEGVAVSNGSACSSGRVQPSHVLKAMGVSDEETTSAI
                     RISTGWNTTQNDIDRFIKVWQNITSRIQKKAQDRA"
     gene            complement(8281..8745)
                     /locus_tag="PK513_05025"
     CDS             complement(8281..8745)
                     /locus_tag="PK513_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO81842.1"
                     /translation="MPSTLTLNSRGEYALIAMVELTRTDSKNPLPLTKIAANAGISLS
                     YLEQLVSGLRRHGLVKSYRGPGGGYLLNKSADEIMITDILRASEDNAPAKRKKTKTGK
                     TNPCEHTHALWEHIGRILSATLCEITLHDVLHNQLEHHPHARKLFETIGKIA"
     gene            9104..9787
                     /locus_tag="PK513_05030"
     CDS             9104..9787
                     /locus_tag="PK513_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO81843.1"
                     /translation="MPEVIFNGPAGRLEGRYSHSKIKNAPLALILHPSPEHGGTMNNK
                     ITYNMFQGFAARGFSCLRFNFRGVGRSQGLFDQGEGELSDAASALDWMQEINPNAPYV
                     WVGGFSFGAWIGMQLLMRRPEIQGFISVAPPANTEDFSFLAPCPTSGLVVHGGKDDVI
                     EESFVSEFVERLHQQKGIDIDYRVIDEANHFFHGYNDVIIDHMHDHLNKAGAGRQVRL
                     EDMVTALAG"
     gene            complement(9855..10334)
                     /gene="bcp"
                     /locus_tag="PK513_05035"
     CDS             complement(9855..10334)
                     /gene="bcp"
                     /locus_tag="PK513_05035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOO81844.1"
                     /translation="MTELKVGDPAPNFTAPADGNRELSLSDFKGQKIVLYFYPKDDTP
                     GCTKESCGFNEALQAFKKLGTTIIGISKDSPVRHDKFKAKYNLNFPLVSDENTDICER
                     YGVWKEKNMYGKKYMGIERSTFLINEEGKIAAIWRKVKVDGHVEEVESAAENIAKAA"
     gene            10424..13588
                     /locus_tag="PK513_05040"
     CDS             10424..13588
                     /locus_tag="PK513_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HOO81845.1"
                     /translation="MDFKLFCRRSGLIFVEILAVGVVLTVVAVCVLGWRLLSAPLDME
                     FARPYIQEALRDEQSGMYVSMDNIVLFWPDLREPLLLGLKGASVYGPEGRVVASIGEA
                     ALGLNKAHLLMGQIRPEGLVLKRPSLSLIRNADNSFDIGVADTKIYGPAQPKEEDGAQ
                     VDYIEQILGVLAGDQNGPVADSLLSELRSFEIEEASLLVDDRVLALSWVLPRADIVIE
                     RVEGALRSKGIVDLPAEAGAAPRLKFTAELGLASEAVDLDAILENFDLSLLGQKIPEL
                     EILKMQDVPLNAQFKARIDKGMKLQRADLTVLSEAGALNIAELSAQPVVYKDLGVLAH
                     YTAQNGQVDLKKVQVTISGVKLEGNADLTLSEAGTVNGPVRLEIERLAQSAIAPLWPV
                     ALAEDNSKEWIVDKLGAGIFSNIYAQGDLEVLPGEGRTSVDLKKLVAGFEFEGMSIDY
                     RAPLKPVTNAKGRARFDLDSETIRIDIESARVMDLAVSEADVELVNIIEAGKGQADIN
                     LKLQGPAKSVFTYIRDEPIGVNSEIDVANVRGQADMRVNISLPTKKDIKIADVDVNVR
                     GTLSNAFLPNVVRGLPLSEGPFDVSVKNNALRVRGKGRLGDQPITLDYHEYLSAAGKP
                     YAMQVKAAVMATPELRDQFGMDLSSFLEGSASVDVTYTEYAGGGAEALVAADLTPARV
                     FVDPFDYEKKPQVKGSATLKAVLKNGDLKEILDLKGSAPALKLESSHLRFRQKGEETE
                     LAGGKVSRFTLNETVAQLEFEVEPSGKTKIVLDGPFLDARPFMDDEKEEGKPYEAPPM
                     LISVAADRMRTADEETVQYAKLYIDIDEQGTINQMEMDGIAGGGDIYLRYKPDGSGKR
                     VFRLEADDAGAILKAFDLYKGVRGGKLVIYGEPIRGVFDRNLIGVAEMSDFKVIDAPA
                     LAQLLGAMSLPGLMASLNGQEGLSFTKMEANFDWRYRPNGSLLVFKDGRTSGNSLGLT
                     FDGTFDNAAHTMDVTGTIIPLSGINKVIGDIPLVGDILTGGTGALIAATYTMKGSSED
                     PKVSVNPLSVLTPGILRRVLFEQK"
BASE COUNT         3186 a         2998 c         3678 g         3774 t
ORIGIN      
        1 taaagcccga aaggattacc cgaagccccg gaagccaacg tcgcctctgt ctcatacact
       61 acaggatgca accccgcatt ttttaaggtg taagccagcg cacacccggc aatcccaccg
      121 ccgacaatcg caatcttcat atctcggtcc cccggtcact caaggaatca ccgtgtttaa
      181 aaaccccaat cgtcatatcg cgcttacgcc cgaacccggg aaccttttca actgcaaaac
      241 caacagcttc caacccacgg cgcaccctgc ccgcagccgt aaaggtcgcc atacggctac
      301 cattatagct aagccgcgcc atattctgaa ataccacatc ggtccacata tccggattgg
      361 aagacggttt aaatccatcg agaaaccacg catcaacctt cgtatcaatt cttgaaatcg
      421 cagcatttac atcgtcaaac accaacgtca aattgacacg cccgctcaaa gacaaacgat
      481 gaaaccccgg cataagtggc ggatataacc gaatcatctt tctaagatgg gcgccaaaaa
      541 acggatccca gggatgcaaa tgcaaatgga tttcctctcc gtccaatgga aatttttcaa
      601 atgaaatgaa atcaagccgt tgatcagacg ttgacgtttc ctcaaaccgc ttccacgctg
      661 ccagaaaatt aagccccgtt ccaaaaccgg tctcggcaat cacaaagcga tcacgcccct
      721 gccagccacg cggcaaatga ttaccatcca gaaaaacata ctccgtttcc gccagcccgt
      781 catcggcaga aaaatacaca tcatcaaatt cttttgatcg cggcgcccct ttggggcttg
      841 caccaaacat agcggcctct atagttattg atcttataag aatttacgcg agtgtaacac
      901 aatgtccaaa aatccgcttc tagaaatttc aaatctgccg aaccacgccc ccgcctttgg
      961 cgccatcaaa aacgaacatt acatgcccgc cgtaaaggcc gccatcgaag aagcccgcgc
     1021 cgtctatgag tcgattaaaa agaaccccga agaaccaaac tttaaaaaca cagtcgaagc
     1081 tatggaaaca gcgggagaaa ctctgagcca ggtcacaagc attttctaca accagctctc
     1141 atccacagga ggtgatgagc ttcacgaact ggctgaaaaa attggcccca tcacctcaaa
     1201 tttctccagc gatattatcc tggatgcggc gctctttgcg cgcctcaaag ccgtacatga
     1261 taaaatagag gagctggacc tgaccgtaga acagcacacc cttcttgaag aagactataa
     1321 aaacttcgtc cgcggcggcg cgctgctccc cgaagataaa aaacagcgcc tgcgcgaaat
     1381 taatgaggcg ctctccaccc tcggcccggc ttttatgaac aacgccaata aatccgccga
     1441 aaaattcgaa atggtcatta aagacaaagc cgatctggcc ggtctgcccg aaagcgctat
     1501 tgaagccgcc gccctgtctg ctaaagaaaa aaacatgaac ggacaatggc gtttcacact
     1561 ggattacccc agctatgccc cgttcattca atatgccgac aaccgcgagc ttcgcgaaaa
     1621 aatgtggcgt gctttcggca gccgcgccta cggtgatgaa tttgataact gcgaaaacat
     1681 caaaaaaatt atcaccctga aagacgaacg tgcgaggctg ctcggttata aaacacatgc
     1741 ccactatgtt ttggaacgcc gtatggccga aaacccctca agcgttttag gcttcctcaa
     1801 aaaacttaaa agtacctata aaccggcagc caaaaaagat cttgctcagc tccagaaatt
     1861 cgccaaaaaa cacggcctcg aaagtgagct taaaccttgg gatgtaggct attatgctga
     1921 aaagctaaaa caaaaactct tcgatttttc ttccgaagac ctccgaccct acttcccact
     1981 ggataaagtc ctggccggat gttttgaaca tttctcacag ctatttaaca tcgagttccg
     2041 cgaagcaaaa gactatgaaa catggcacaa agacgtcaaa acctttgaac tttataacaa
     2101 aacctccggg gccttcatcg gcacactttt cggcgacttc ttcccccgca caggtaaaaa
     2161 gaacggcgca tggatgaccg cctatcgcag ccagggcctg ttccatggca agatcgaacg
     2221 cccggtcatt ggcatcgtct gcaacttcac aaagcctacc gaagacaaac cctcgctcat
     2281 tacccacggc gaagtcacaa ccttgttcca tgaaatgggc cacgccattc acggattgct
     2341 ttcaaacatc acctatcgct ccttatcagg cacctcagtc ctctgggatt ttgtcgaact
     2401 cccctcccag cttcaggaaa actggtgcta tgagaagaaa accctcgacc atttcgccgc
     2461 ccattacgaa accggtgaaa aaataccggt caaccttatc gaaaaagtac gcaaggccaa
     2521 aaacttcatg gtcggctggg gcggtttgcg ccaaatcggc ctcggaatgc tggacatgac
     2581 ctggcacacc gccgacccgt caacaatcga cgatgtcgcc gcattcgaag ataaagcgct
     2641 taaagataca acgctcttcc cacgcctcgc cggaccgacc tcaaccagct tcagccatat
     2701 cttcgcaggc ggctattccg ccggatatta cagctataaa tgggccgagg ttctcgacgc
     2761 cgatacgttc gaactgtttc ttgaaaaggg cctgtatgac cgcaaaaccg ccaaagccta
     2821 tcaggaaaaa atcctgtcca agggcggttc ccagcatccg gcaatcctgt atcggaattt
     2881 ccgtggccgc gacgcagacc ccgacgccct gctccgccgc gaagacttgc taaaagataa
     2941 ggcagcgtaa ataaaagccg tgtgccgttg cgaaaatggt tttggacgat ttttctgatg
     3001 gcgacgtcaa atgacggaca aggctgattt tagctttgaa ggcggggttg gtagcggtgg
     3061 ggtgatttga acacccgaca caaggattat gattcctctg ctctaacccc tgagctacac
     3121 cgccgtaacc taaatggcca agaagaccga tttgttactc caatgatacc cgtaaagggt
     3181 agggttatgt tttttatact ttccaaggct tgtcatttca agccgtttgg agcgcgcctc
     3241 atttgagaca tatggatttg ttccactgct ttaaccggct gagatacttc gccataagcg
     3301 ctgttgacgc tgcaaggttg gtttatcgcg ttttcatttg aaattgtcaa gagaggcgta
     3361 tttaaaattg ccgcgtggcc tgaaaaaggt gcggggttac agagagttgt tttttattgc
     3421 ggcactattg gggtgaaatt gtgcgggcca ccgaaattaa acataccggc ttctttgact
     3481 tcaaggccga atttatcggg tctgctcatg atatcttgaa atgcagcttc tatggccgga
     3541 tgtcgtcctt ccggtcctct gtcatcgagt tcttccatca tggttgctgt aatgcgttgt
     3601 gggcttgtat atgctgtgtt gttgtcggca aagagttttt gtctttcata gaggcgcaag
     3661 aatgtttcct cgatatgatc caagggaagt gccttagtgt tgaattgatc cgttacatct
     3721 ttagggtagt ctgctttggg actatattta tttttctgga atttttccgc aggcataatt
     3781 ccttgttcga ttatgcgcgt atttgtccgt cctaaagctg tatattttgt atcgcccatg
     3841 tcaaatgccc tcttgagcgt atctcgctct ttttgacata ataacatttc agatgttaaa
     3901 aattgattaa ggtaaattac ggattatggc tgtgagcaat gtttttagtt gagtttttga
     3961 tggtggttat ttttaggagg atggtcccgg gagttccatt ctgttgtcag cggcggtgct
     4021 gtcgtggctg ccgaaccatc catcttcaat gcggtatttt gcttgattgg cctcgacttc
     4081 ggcgatggct gcatttactt cgtcgcttat ttcttcgggg atgagccctt tcttagccat
     4141 tgttatgaca tcgtcaaaat tattagcgtt gatcccccat gatgaaattt cactgatgac
     4201 cccgccatcg cgttcaagag cttcgttaat gtcattatat tggttcatga aggtgcaaac
     4261 cgggtcggca catttggtga attctgcctt tgggccttcg actttaatct ttgaaccaac
     4321 agaaggttcg cggcctattt ctttatagat ttcggccagt cggtttcgtg tgtctttgct
     4381 aacgcctgcg ctgttatagc ttgctaatgt gatgtcccct ggttccgcca tgtgtcaagc
     4441 cttttctggt taagttacgg ctttattata cataatttat tcttaagggg tggttaacgt
     4501 ggcaggaaag gcgactttgt ggcgggagat atagaattgt ctgaaaatgc tggcttaagc
     4561 agcgttttgc gcggattttt tgcttgtttt tgtgatccag ccgccgccga ggacgcgatc
     4621 gcccgcatag catacggcgg cttgccccgg tgaaatgccg tactgtggct ggtctagtct
     4681 gatttcattt ttttccgtgt ttaaaacggc gggtgtaggc tgcatgacag agcggaactt
     4741 cactgtgaca gggaggaatg cgcagccttt ttttgccttc aggtttttgg tgtcatacag
     4801 ccagttgcaa tcttctagat acagaatatc gcgggccagg gcttctttcg ggccgacgat
     4861 gacggtgttg gtcgcagggt cgatctgcac gacatataag ggttcgttgt tctcgctgac
     4921 gccgccgccg atgccgaggc ctttgcgctg gccgatggtg tagtggatga tcccctcgtg
     4981 cgtgccgacg gtgcggccat cgatatggac gatgtcgccg ggctgttccg cttcggggcg
     5041 gatttttttg actacacgcg catagtcgcc atgtgggacg aagcagatgt cctgagagtc
     5101 gggtttgtcg gcagtgatca ggccgaggcg gtcggcctcc cgacgggttt tgtctttgct
     5161 ccagccgccc aagggaaagc gcaggaagtc gagttgttcc tgcgtggtgg cgaagaggaa
     5221 atagctctgg tccttgccgt ggtcgtgggc gcggtggagg gtggcgcggc catcgtcgtt
     5281 gattacgcgt ttgatgtagt ggccggtggc catgcaatct gcgccgaggt ctttggccac
     5341 ttgcagcaga tcgtggaatt tgacgctctg gttgcatttg acgcaaggga tcggggtttg
     5401 gccgcgcata tagctatcag cgaaatcctc gatgacgctt tcgcggaaat tgctttcgta
     5461 attcagaaca tagtgaggga agccgcaaat acctgcgact tttttggcgt cgtagatatc
     5521 ctgtccggcg cagcaggttt tcgatttggc gaccgcttcg cccccatggt cattggagtc
     5581 atagagttgg agcgtaacgc cgacgacatc atagccttgc tcatacaaga gcgcggcggc
     5641 gacggaggag tcgacgccgc cggacatggc cacgactacg cgcgtgtcct tgggggcttt
     5701 gtttatgcct aggctattca taacgggggt tatataagcg tttgccgtga aaaagtccag
     5761 atttccgagt ttttgttgac atattataaa atcaaatata gtgagtgtgc catgttttca
     5821 tatattagac gccagatatt acaaaaacgg cttggttttg cttttaagca aaaagagttg
     5881 ccgattatcg atcaaaatcc tgaattcttc ttttcggggg attatttgcg tcatgcgcgt
     5941 gaaacgaaag agcgcattga gaaaataatt cttgaaaaag ggtttgaggg caattttgat
     6001 cttgtgctgg ttgatgaatt taatcattgc gcgcgtaaga ttaagccaat ttacggcagg
     6061 gagactacag ttttgcagga ttttatggct agtcagaagg atagctacca aacgctgcgg
     6121 gattttttta ctgaagctaa agatgttctt gtttacgagg cttcgagttt gcgtgatttt
     6181 gcgtattctc agcttacagt ggcatcaaag aaggctcttc gggaagagct tgggaaaata
     6241 tcgtcatcag atttttttga ggctacgcct ttgtttcaag cctttcattt ttggaaagct
     6301 gctatggaat caaattattt gaataaagat agaggcaatt gtgatttttg gggagactct
     6361 ttgcgtgtgt cgtgcatgcg cggcgaaggt gatttgatgg ttcgctttta tgaatctgtt
     6421 tataatctcg atgtgtacgg gaatgagcct cctgaaggac atcgccgccg ggatgaagag
     6481 cttttgaagg gcgtgctggg cggttttgat tgggacgccg ccgttccctc tcattaggtt
     6541 tcaagttgca gcctcaatat ccgcggcgaa gcgtagttcg cgcaagggac ggacgatgga
     6601 ggtagatttt tcgtagagcg ggtggcgttt ataggcgttc agtgtttcaa gatcggggaa
     6661 ttcaccatag acgacaatat cgatgtcgtt gccgagctga tcggcttttt gattgcgggt
     6721 gatctctatt tttgcgccgg gaatttgcgt gaggttcatc agcccgcttt cgattgtatc
     6781 gaggtcatcc ttgttcttgg cggaaaacat tacaatatgc cggatcatga aaatgcctct
     6841 tcttttgtgc ctggcaaggt gacgaccagg ccgtcgagat cctcagtgag ttttacctga
     6901 catccaagcc gagaagtgtc acgtacatcg aaggccatgt cgagcatgtc ttcttcttcc
     6961 tcgcattgtt cgttgcccgc ggcggttacg cgatcgatcc agtcagagtg gatatagaca
     7021 tggcaggtgg cgcaggccat tgatccgccg caggcaccct ctataccttt gatcttatgt
     7081 tcgacggcaa cctgcatgat cgaccagttg ttttcagctt ctataatctg gtgcgaaccg
     7141 tctttatgca ggaatgtaat tttaggcacg gtcttgagct ttcttttgga tgcgggaggt
     7201 gatgttttgc cagactttga taaagcgatc aatatcgttt tgggttgtgt tccatccggt
     7261 cgagatgcgt atggcgcttg tggtttcttc atcgcttacg cccatggctt tgagaacgtg
     7321 gctgggctga acgcggcccg atgagcaggc tgagccgttg gatacggcga cgccttcgag
     7381 gtcgagcgct atgagcaatg tttcggagga tacgccgggc tgggagaaca gcgtggtgtt
     7441 cgcaacgcgc ggggcatttt tgccgtagat ttttatgttt tttaacgtcg tttcaagttt
     7501 ggtttgcagg gcgcgtgttt tgttctgata atcctgaatt ccgtcttggg ctaactctgc
     7561 tgctatccca aatccggcaa ttccggcgac attttcggtg ccggctcttg cggatttttc
     7621 ttgcccgccg ccatgcagca gggttggtgt tattccgcaa atgccgagtg cgagggcccc
     7681 gataccttga gggccgccga ttttatgggc tgagagggta aaaaaatcga taccaagggc
     7741 ggccatatcg atggggatac gcccggcggc ctgcacagca tcgcagtgaa atagcgcgcc
     7801 gtgtttgtgg gccagcgctg cggcttcttt gacgggctgg ataatgccgg tttcattatt
     7861 ggccagcatc accgaaacta atgcggtttt ttcggctttg agtagtatct cgaaggtttc
     7921 cagatccagc acgccttccg gagtagtcgg gatgcggtca cttttcggga cagtttccaa
     7981 aacggacgga tgttcaatgg ctgatacgag aacccgttct tttgtgaaat ggcgcaggac
     8041 tgtgttattg ccctctgtcg cgccggaatt gaaaatgatt tgagcaggcg ggacaccgac
     8101 gagggcggca attttctcgc gggcacactc gacaatttta cgtccttcgc ggccaaagct
     8161 atgtacagca gaagcgttgt gcggcgcgct taagcattca tttataatgg cgatgacttc
     8221 tggccgcgtt ggcgcggtgg cgttgtaatc taaataggtt tttttatcca tggacgctat
     8281 ttaagcgatt ttccctatgg tttcaaatag tttacgagca tgtgggtgat gttcaagttg
     8341 attgtgcagt acgtcatgta atgtaatttc acacagagtt gccgataaaa ttctaccaat
     8401 atgctcccat agggcatggg tatgttcaca aggatttgtt ttgccggttt tcgttttttt
     8461 gcgttttgcc ggtgcgttgt cttcgctggc gcgcagaata tcggtaatca tgatttcatc
     8521 ggcactcttg tttaaaagat acccaccgcc ggggccgcga tagcttttga caaggccgtg
     8581 gcgtcgtagt ccggagacga gttgttcaag gtaggatagt gaaattccgg cattggctgc
     8641 aatttttgtt aatggaagcg gatttttaga gtctgtacgg gtaagttcaa ccattgctat
     8701 gagggcgtat tctccgcgtg aatttaatgt gagtgttgag ggcattcgtt tttacggttt
     8761 taaattttcg tttatattaa taatgtctaa tgctttatag gttctttggc gtttttgcac
     8821 tatgaaattt tcttttttgt cgtatgtttt tgtgaatttt gtgaaaaagg gcggctgttt
     8881 ttttacatag tacatatttt gagctacttg gactgaaaat gattcgattt gtagcgggta
     8941 ttatccacaa acagaatcaa taggttgtaa tgtttttata gacagtgcag gatgtttttc
     9001 ctatataggg gcagctttaa aatatagcag gatcggggcg aggcttttat ttccttcgtt
     9061 ccggttttta aaaatcaaac ccgaaaacag ttagggatac taaatgccag aagttatttt
     9121 caatggtccg gcgggccgtc ttgaaggtcg ctattctcat tcaaaaatta aaaacgcccc
     9181 gctcgcttta attttacatc cgagtccaga gcatggcggt acgatgaata acaaaatcac
     9241 ttataatatg ttccaaggct ttgcggcgcg cggattttcg tgtttgcggt ttaatttccg
     9301 tggtgttggg cgttcgcaag gactgtttga tcaaggcgag ggagagctta gcgatgccgc
     9361 ttctgcgctg gactggatgc aggagattaa tccaaatgcg ccgtatgttt gggttggcgg
     9421 gttttctttt ggtgcgtgga tcggtatgca gcttttaatg cggcgcccgg aaattcaagg
     9481 ttttatttct gtcgctccgc ctgcgaatac tgaggatttt agttttcttg caccgtgccc
     9541 gacttccggt ctggtggtgc atggaggcaa agatgacgta atcgaagaat ccttcgtgtc
     9601 tgagtttgtt gaacgtttgc atcagcaaaa aggtattgat atcgattacc gcgtgattga
     9661 tgaggctaat catttcttcc acggctataa tgacgtgatt attgatcata tgcatgatca
     9721 tttgaacaag gctggtgccg ggcgtcaggt tcggctggaa gatatggtca ccgctctggc
     9781 gggatagtca tagtcctttg tgatcaccaa atttttctaa atagaaaagc cctcaatcgt
     9841 cgagggcttt ttgtctaggc ggcttttgcg atgttttctg cggcgctttc aacttcttca
     9901 acgtggccgt cgactttgac tttgcgccat atagcggcga ttttgccttc ctcattgatc
     9961 aggaaggttg agcgttcaat gcccatgtat tttttgccgt acatattttt ttctttccag
    10021 acgccatagc gttcgcaaat gtcggtgttt tcatcggaga cgagcgggaa attcaaattg
    10081 tattttgctt tgaatttatc atggcgtact ggtgagtctt tggagatgcc gatgatggtt
    10141 gtgcctagtt ttttgaaagc ttgcagggct tcgttgaagc cgcaactttc cttggtgcaa
    10201 ccgggtgtgt cgtctttggg atagaaatat aggacaatct tttggccttt aaaatccgat
    10261 agggaaagtt cacgattgcc gtctgcgggg gcggtgaaat taggggcggg gtctccaact
    10321 tttaattctg tcatagcgtt gtcctttcaa aaatccatac ttacttcagt ttctttaaca
    10381 tgggggcttt gcggcttaag tcaagcgtgg cataaaatag gatatggatt tcaagctttt
    10441 ctgcagacgt agtggtctta tattcgttga aattttggcg gttggcgttg ttttaacggt
    10501 ggtggctgta tgcgttttag gatggcggct tttgtcggcg ccgctggata tggagtttgc
    10561 gcggccatat attcaagaag ctttgcgtga tgagcaaagc ggtatgtatg tcagcatgga
    10621 taacattgtg ctgttttggc cggatttgcg cgagccgttg ctgctgggcc taaagggggc
    10681 gagcgtgtat gggccggagg ggcgtgttgt ggcttcgatt ggcgaagcag cgctggggct
    10741 gaataaagcg catttgctga tggggcagat acggcccgaa gggttggttc ttaagcgtcc
    10801 aagtttgagt cttattcgaa atgcggataa tagctttgat attggcgtgg cagatacaaa
    10861 aatttatggt ccggcgcaac caaaggaaga agatggcgcg caagttgatt atattgaaca
    10921 aattctaggc gttcttgccg gcgatcagaa cgggccagtg gcggatagtt tgctttcgga
    10981 actgagaagt tttgagattg aagaggccag cttgttggtt gatgaccggg tgctcgcgct
    11041 ttcgtgggtt ttgccgcgcg ctgatattgt gattgaacgg gtcgaggggg ctttgcggtc
    11101 gaaggggatt gtggatttgc ctgcagaggc gggcgccgcg cctcggctta aatttacggc
    11161 agagttagga cttgcctctg aggcggtaga tctggatgca attttagaaa attttgattt
    11221 gtcactgttg gggcagaaaa ttcctgagct tgagattttg aaaatgcagg atgtgccgct
    11281 caatgcacag tttaaggcgc gtattgataa aggtatgaag ttgcagcgcg ctgatttaac
    11341 ggtgctttct gaggccgggg cgttgaatat tgcagagctt tcggcacagc ctgtggtcta
    11401 caaagattta ggcgttttgg cgcattacac ggcgcagaac ggtcaggttg atctcaagaa
    11461 ggtgcaggtt acgatcagcg gggtaaagct tgaggggaat gccgatttga cgttgagtga
    11521 ggcgggcact gttaacggtc cggtgcgtct tgaaattgaa cgtttagcgc agagtgcgat
    11581 tgcacctttg tggcctgtgg cgctggccga ggataacagc aaggaatgga ttgttgataa
    11641 gctcggggca ggcatttttt caaatattta tgcgcagggc gatcttgagg ttctaccggg
    11701 tgagggtaga acgagtgttg atctgaagaa gcttgtggcg ggatttgagt ttgaaggtat
    11761 gagtattgat tatcgtgcgc cacttaagcc ggttactaac gctaaaggca gagccagatt
    11821 cgatctcgat agcgaaacga tacgtattga tattgaatct gccagagtga tggatttggc
    11881 tgttagtgaa gcggatgttg agttagtcaa tattattgag gctggaaaag ggcaagccga
    11941 tattaacctt aagcttcaag gtcctgctaa atcagttttt acctacattc gtgatgagcc
    12001 gattggggtg aatagtgaga ttgatgttgc gaatgttcgt ggtcaggctg atatgcgggt
    12061 taatatttcc ttgccgacga aaaaggacat taagattgcc gatgtcgatg tgaatgttcg
    12121 aggaacgctc agcaatgcat tcttgcccaa tgtggtgcgc ggacttccct taagtgaagg
    12181 gccgtttgat gtaagcgtga aaaacaatgc tttgcgggtg aggggcaagg gacggcttgg
    12241 cgatcagccg attacgcttg attatcatga gtatttaagt gcggcaggca agccctatgc
    12301 catgcaggtt aaggctgcag tgatggcgac gccggaactg cgtgatcagt tcggcatgga
    12361 tctgagcagc tttttagaag gatcggcttc ggttgatgtg acctatacag aatatgctgg
    12421 ggggggcgcc gaggcgcttg tggcggcgga tttgactccg gcgcgggtct ttgtcgatcc
    12481 gtttgattat gagaagaagc ctcaggtgaa gggaagcgca acgcttaaag cggttttgaa
    12541 gaatggggac ttaaaggaaa ttcttgatct gaaagggagc gcgccggctt tgaaactgga
    12601 gtcttctcat ttacggtttc gtcagaaggg cgaagagaca gaattggctg gaggcaaggt
    12661 ttcacgtttt acgttgaatg aaacggtggc gcagttggaa tttgaggttg agccttccgg
    12721 aaagacgaaa attgtgctgg atgggccctt ccttgatgca aggccgttta tggacgatga
    12781 aaaagaagag ggtaaacctt atgaagcgcc gccgatgttg atttcggtgg cggctgaccg
    12841 gatgcgcacg gcggatgaag aaacagtgca atatgccaag ctgtatatcg atattgacga
    12901 acagggcaca attaaccaga tggaaatgga tggtattgcg ggcggcggcg atatttattt
    12961 gcgttataag cccgatggct ctggcaagag ggtctttcga ttggaagccg atgatgcggg
    13021 tgcgatactt aaagcatttg atttgtataa aggtgtgcgc ggcggcaagc tggtgattta
    13081 cggcgagccg atccgcggag tgtttgaccg gaatttaatc ggtgtggcgg agatgagcga
    13141 tttcaaggtt atcgatgctc cagcattggc gcagcttctc ggggcgatgt ctttgccagg
    13201 gttaatggcc agcctgaacg ggcaggaagg cctcagcttt accaagatgg aggcgaattt
    13261 tgactggcgg tatagaccga acgggtcgtt gctggtgttt aaagatggcc ggacctcggg
    13321 aaattcattg gggctgacct tcgacgggac gtttgataat gccgcccata cgatggatgt
    13381 gaccggtaca attattccgc tgtccgggat taacaaggtg atcggcgata ttccgctggt
    13441 cggggatatt ttgaccggcg gaaccggcgc gttgattgcg gcgacctata cgatgaaagg
    13501 atcaagcgaa gatccgaaag tctcggtcaa tccgctgtcc gtattgacgc cggggatctt
    13561 gcggcgcgtg ctgttcgagc agaaataggc cattttgggg tgtcttttaa cccactgttt
    13621 ttatgtatta aagttc
//
LOCUS       DAOSWR010000048        13554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2882_length_13554_cov_5.062894, whole genome shotgun sequence.
ACCESSION   DAOSWR010000048 DAOSWR010000000
VERSION     DAOSWR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_13554_cov_5.062894"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK513_05045"
     CDS             complement(<1..559)
                     /locus_tag="PK513_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOO81846.1"
                     /translation="MHRPSLRQLSYLIALRERGSFIAAAEECAVTQSTLSAGIKELEI
                     ILSQPLVIRGRKATHLTPFGLEVYDNAQKIINDTDRIVARSKLISQPLSGPLRLGIIP
                     TIAPYMLPDMLPKLIEKFPRLELQIHEDLTDRLLESLRKSQIDLALLAFPYNTPDMHQ
                     RILFEEKFYAAVGKHAGPAANSINIK"
     gene            704..1285
                     /locus_tag="PK513_05050"
     CDS             704..1285
                     /locus_tag="PK513_05050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOO81847.1"
                     /translation="MLGIGDQIPEFEITGVKPGFNVHEENGESAFEAITEKSFEGKWK
                     VFFFYPKDFTFICPTEIVEFAKLNDEFADRDCVVLGGSTDNEFCKLAWRREHPDLSKL
                     NQWSFADTNGSLIDGLGIREMQAGVALRATFIVDPHGVIQHVAVNGLNVGRNPKETLR
                     ILDALQSDELCPCNRAVGGETIDVHKEAQKIAA"
     gene            1355..>1739
                     /locus_tag="PK513_05055"
     CDS             1355..>1739
                     /locus_tag="PK513_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HOO81848.1"
                     /translation="MSIDNLKNKMPDYAKDIKLNLSSLANEETLNSQQLWGAFVATAH
                     AGRNVAVIREIEEEAAQYLSPEALEAAKAAAAIMAMNNVYYKFTGMMADYQAIPAKLR
                     MNIIGNPGVDKVDFELWSLAVSAING"
     assembly_gap    1740..1839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1840..2055
                     /locus_tag="PK513_05060"
     CDS             <1840..2055
                     /locus_tag="PK513_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO81849.1"
                     /translation="VDKVDFELWSLAVSAINGCQFCVKSHEKKIREGGLSLEQVQTAV
                     RIAAVVQAVSAVLDGAAALGGDVAQAA"
     gene            2173..3045
                     /locus_tag="PK513_05065"
     CDS             2173..3045
                     /locus_tag="PK513_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81850.1"
                     /translation="MGRIDQSTVDTSFVPERHPGARARWAIQIATVDSFKWRGSHKLA
                     SFLGRNSVEHSFTRMAQIWCDEDGKPLKDPETGRLYPQICVRGELNPGAIDPKTGEWT
                     GLRIDPHTVGNIVSTFIPRRLMDAFNNAVFKSKKCKLGVFSWYGDGIEPLDADYVVEE
                     PVAISDDQDCIEALWAVYQERAEDINRQGLDYDLVRRNCHTVNAVLNAGNVEAVERFA
                     KRGFMRWGAKTDAIKVAEDTPPVVRSLEELQKLNCNWADGVCMSCQRLAGRGQLHSEE
                     LQDGSHELSVEYAG"
     gene            3146..3781
                     /locus_tag="PK513_05070"
     CDS             3146..3781
                     /locus_tag="PK513_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81851.1"
                     /translation="MRFGVTNDALCGLYERFGLDLDDERDGPVQMLYEETITDDDPEI
                     GVRQFLGNVSLDKVLQCQLFSKEDYNRFLGPPLGLADYLEHLKGEMQDHREDHTRSIE
                     IIDQLIPRTESTEDLVFKGYLGRYQVSWSASPIMRFGTTGMSDAPLSLDKPQDCMNAL
                     ERIEYGHDSFFEGRVLAVLSRFKGVDWPGIQIEVEPYEEGPVFEPEGTPVP"
     gene            complement(3784..5082)
                     /locus_tag="PK513_05075"
     CDS             complement(3784..5082)
                     /locus_tag="PK513_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO81852.1"
                     /translation="MLENINIEEYWNIITNWCANHFWNTDTLYQGILLLIAFVLASIS
                     YSMFRSKAAKLIEKTKMPYAARRISNNLLRLLLPVVALIILVIGDQLLKSDIMGVNTA
                     LTEGVMKVLLAWVAIRTAVQFIANSAIRNFFALVIWTIAALSIFGLLHQTMTTLDSIS
                     FSIGDFRLSALIVIKSILSIFTLLYLAIFASSFAERRIFRIKSLTRSSQVLIAKVIRV
                     ALIVFALLVGVTSAGIDLSLFAVFGGAIGLGIGFGLQKGISNLFSGMLLLMDQSIKPG
                     DVLELENGTFGWVNQMAARYTEIVTRDNKSFLIPNEELITQRVVNWSHGNSLIRLQVN
                     FGTHYNSDPHKVIEIAEAVAAAAHERIHKDPPPVCHLTEFGDSSINFSVRFWIKDAEA
                     GVTNIKGAVMLALWDAFKENAIEIPYPHREVYVHEVKKGA"
     gene            complement(5169..5792)
                     /locus_tag="PK513_05080"
     CDS             complement(5169..5792)
                     /locus_tag="PK513_05080"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO81853.1"
                     /translation="MSQAALQKNNAQTTPTMQARKGRPRSQKSKTAILKATNSLLLHM
                     SVQELSIEAIAKKAKVGKTTIYRWWPNKTAVVMDALRAQPGIQSPLPTPKNNTEAIIM
                     QLDKLIRMLDGNNGDTIAQLFSEAQADDKAQKIFARTFLTPLIDAVEFSIEQGKDHKE
                     FRSSLNTKLAVDLLCGPIFFRLMSRPADLNEEFRETYPKEAVRLISA"
     gene            complement(5990..7279)
                     /locus_tag="PK513_05085"
     CDS             complement(5990..7279)
                     /locus_tag="PK513_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOO81854.1"
                     /translation="MTQRFKTAFIALFLIAPLLVLGACQTGSGYRPHTSVPQAPAQQA
                     PGILSSTSSDELLGNQQNPITPTDHMINLPTVKVGILLPLSGQHQKLGDSMLKAAQMA
                     LFEIGHANLELIPRDTKGTAAGAQAAARSAIDSGAQLLLGPVFAPAVRSAKPVAQRAH
                     INMIAFSTDWTLANSNTYVMGFMPFDQVKRVTSFAARHGITRVGVLAPDTNYGRIVSN
                     AFNAAAPHYNITITENISYPAGTSNLAPIVKTFTHYEARKSAGALAQTPFDAVLMPVG
                     DQDARAIASLLSHYNLPPRSVRRIGTGLFDEASLASEANLEGAWFAAPDPAARHSFEQ
                     RFINTYGTHPPRLATLAFDATALSAILARRGLQSTGRPAFDHTAITNPNGFSGLDGIF
                     RFRQDNTAERGLAVLEFRRGRLGVIDQAPKSFQNMTQ"
     gene            7315..8217
                     /gene="rsmI"
                     /locus_tag="PK513_05090"
     CDS             7315..8217
                     /gene="rsmI"
                     /locus_tag="PK513_05090"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOO81855.1"
                     /translation="MSEKLSQSESKEKKRGWLLPDDAGRGLTPGLYLVATPIGNLRDI
                     SLRALDTLSQADGVLCEDTRVSGKLLKHYEIAQKLSVYNDHSDETVRAKIVARIGRGE
                     ALALISDAGMPLVSDPGYKLVADVQRAGLNVTSVPGANAVLAGLQLSGLGSDRFSFMG
                     FLPSKSAARRSILCEWQGSPGTLIAFETAPRLLAALDDIESALPGRRVAVVRELTKLY
                     EEVRCDDVAALRAHYEERGPVKGEIVLVIEAAQVRTYSEDDLKALLEKALKEMSTRDA
                     AAQVAEVSGQPKKVLYALALEISK"
     gene            8214..8615
                     /locus_tag="PK513_05095"
     CDS             8214..8615
                     /locus_tag="PK513_05095"
                     /inference="COORDINATES: protein motif:HMM:NF009151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOO81856.1"
                     /translation="MSRLTKEAAYQRGVAAEQAAARYLQRQGYKILEQRYKTRVGEID
                     LIVRRGEMLVFVEVKAHKDPESALYAVTPRTRRRIEGAAGQYLADHEEASGLSMRFDV
                     IVVPPESLRKGLNLLGAVSIRHLDNAWLSGQ"
     gene            8654..9517
                     /locus_tag="PK513_05100"
     CDS             8654..9517
                     /locus_tag="PK513_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOO81857.1"
                     /translation="MVRKFLILSVCVLFLPGCSALGVAAGVGATAGIAAAQEGGLSAA
                     VDDARIQIEINDLWFKYDVETFRKLDLTINQGRVLITGVVQDPEARVEAVRLAWQPKG
                     VKQVINEIQIAESAGIVGFAKDTWITTRLRTAITFDREILSINYSIDTVRGTVYLMGF
                     AQNRSELNRVIEIARTINGVNGVVSYVKFVGGKTQTGGVDDGSTYQSTYDDNYVYQNA
                     QGQSVPVRPYEDSNMPIDLRGSQPSPDYGTDSSSYSNNVDPMFSQEPSAPARPTQRQG
                     IESEEILWNDQ"
     gene            9507..10373
                     /locus_tag="PK513_05105"
     CDS             9507..10373
                     /locus_tag="PK513_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO81858.1"
                     /translation="MTSNPSGLEELSGAFDPESYIQSLGSIPDDRINLAHAALALAAL
                     SHEGVSLGRYIYHLQVIGDEVGARYAEIFGDSAKAQLEALSDILHGRYHYSGDRESYD
                     DLQNASLIRVIDRAKGRAIVLSILYIHSARAQGWNIVGLDVSGHFLCRLEAGGQRIIF
                     DPFEGGRIVEAQDMREIVKRVFGAQAELSSEYYEPVGNRGILVRLQNIIKFRQIEAED
                     YQGALETVQRMRQIDPQEYRLLLDEGVLLARTGEPRGAIDSLVAYIEKAPNSRDRMEA
                     ELLLQQLNDALN"
     gene            10540..11109
                     /gene="pal"
                     /locus_tag="PK513_05110"
     CDS             10540..11109
                     /gene="pal"
                     /locus_tag="PK513_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOO81859.1"
                     /translation="MTLSLFRKVSKDSFALSRLMMILAVVFLVSACSSNDDEEVIFDG
                     QSAAAREGITDGPLDEIYQGGAQQGPAPGTQADLVVNVGDRIFFGTDRYDLNNEARMI
                     LDKQAAWLEQYSSLNVTIEGHADERGTREYNLALGERRANSVRNYLSALGIAPSRIQV
                     ISYGKERPAVPGANESAWSQNRRSVTKID"
     gene            11134..12102
                     /gene="ybgF"
                     /locus_tag="PK513_05115"
     CDS             11134..12102
                     /gene="ybgF"
                     /locus_tag="PK513_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOO81860.1"
                     /translation="MTILSFYIRKRRAWIFSGLLAVALAGLPFMAGAQGGDWDMQNRI
                     KRLENEIQTLSRAVYRGEKPPAGVGGGLSGGADAEIRLQQIETEVRGLRGDLEEQSFE
                     IRELKDQLERFTSDMELRMNDLEGSGASRSGSAAPVSGASRYTARPLRDSNPPSVVGS
                     GVGQTYQWSSSNANSGGAVGQLGSYTESGDGTSSSDLASATYENAFSMVKNHQYKGAQ
                     VEFESFLRDHPDHPLAGNAKYWLGETHYVRGNFETAARIFAEGYQQYPKGSKAADNLL
                     KLGLSLDALGKKDDACVALRQLKKENPSGAAPVMRRADQEMSRIGC"
     gene            12152..13150
                     /gene="tilS"
                     /locus_tag="PK513_05120"
     CDS             12152..13150
                     /gene="tilS"
                     /locus_tag="PK513_05120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOO81861.1"
                     /translation="MNFEDFNHRMLALGVGGTGPIAVAVSGGPDSMALCWLLSRWADG
                     RGVYVHALTVDHGLRPEAADEAVQVGAWLDGWSQQVGHHILRWETPADTAIQEEARRA
                     RYGLMAEYCECHDIEYLFLAHHRDDQAETVLFRLAKGSGLDGLSGMQECQAYDERLTL
                     VRPLLDCGKAELLKLCGAEDISYFEDPSNANSDFARVRLRKARCVLEEEGLTSKRLAV
                     TAQRLGRARRALEVISAQAYQNAAIESNTKHIVFKINILFMQPEEVILRCLLKALSVL
                     RPLSDYAPRMERVEVILYDLMAVKNKGTPFRKRTLGGVMFAMDENLGILRLTREYS"
     gene            13313..>13554
                     /locus_tag="PK513_05125"
     CDS             13313..>13554
                     /locus_tag="PK513_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOO81862.1"
                     /translation="MNGFGRNLIFWLAIGLTMIFLFNIFQGAQQGAINRGNKISYSEF
                     MTDAQAGRVSNVVIKGQELHGVFASSGEEFKVMVPP"
BASE COUNT         2996 a         2446 c         4257 g         3755 t
ORIGIN      
        1 ctttgatatt gattgaattt gcggctgggc cagcatgctt gccgacggcg gcgtagaatt
       61 tttcttcaaa caaaatgcgt tgatgcatgt ctggagtgtt atacggaaag gcgagcaggg
      121 cgaggtcgat ttgtgatttt cttaatgatt ccagtagtct gtcggtaagg tcttcgtgga
      181 tttgtagctc caggcgggga aatttttcaa tcagttttgg gagcatatcg ggaagcatat
      241 agggcgctat ggtggggatt atcccgaggc gcagtggccc gctaaggggc tgagatatta
      301 gctttgacct tgctacgata cgatctgtat cgttgataat tttttgtgcg ttatcataga
      361 cttcaaggcc aaatggcgtg aggtgcgtgg ctttgcgtcc ccggatgacg agtggctggc
      421 tgaggatgat ttcaagttct ttaatacctg cgctcagcgt gctttgtgtt acggcacatt
      481 cttctgctgc ggcgatgaat gatccgcgtt cgcgtagggc gatcagataa cttaattgtc
      541 tgagactggg tctatgcata tatcgaaaat atcatatcaa tgtgtatttt agtatttatt
      601 ttttcgatat taataagtgg aaatttttgt tggattttgt cgttctgagg gtgcaagtta
      661 cgggcatgga tttttaaccc gaacaaagaa tggagattta gaaatgctcg gtattggtga
      721 tcaaattcct gaattcgaaa ttacaggtgt gaagcccggc tttaacgtgc acgaagaaaa
      781 tggcgaaagc gcgtttgagg cgatcacgga aaaaagtttt gaaggcaagt ggaaggtttt
      841 ctttttctac ccgaaagatt ttacgtttat ctgcccgaca gaaatcgtcg aatttgcaaa
      901 gctgaatgat gaatttgcag accgtgattg tgtggttttg ggtggttcta cagataatga
      961 gttttgtaaa ctggcatggc gtcgcgagca tcctgatttg tctaagctga accagtggtc
     1021 atttgctgat acgaacggtt ccttgattga cggtttgggt attcgtgaga tgcaggctgg
     1081 cgttgcgttg cgtgcgacat tcattgtcga tcctcatggc gtaattcagc atgttgccgt
     1141 gaacggcctg aatgtgggac gcaatccgaa ggaaaccttg cgcattctgg atgcgttgca
     1201 atctgatgag ctgtgtccgt gtaaccgtgc ggttggtggc gagacgattg atgttcataa
     1261 ggaagctcag aagatcgcgg cttaattaag gttcgcaggc ggcaggtttg aagaactttg
     1321 ccgcctgttt tgtcttttag agaaaggaaa aacgatgtct attgataatt tgaaaaataa
     1381 aatgccggac tatgccaagg atattaagtt gaatttgtcg tcgctggcga atgaggagac
     1441 gctgaactcg cagcagcttt ggggggcgtt tgtagcgaca gcgcatgccg ggcgcaatgt
     1501 ggctgtaatt cgtgagattg aggaagaagc ggcgcaatat ctttcgcctg aggcgctgga
     1561 ggcggccaag gcggcggcgg cgattatggc gatgaataat gtttattata agttcaccgg
     1621 catgatggcg gattatcagg ccattccggc gaaattgcgg atgaatatta tcgggaatcc
     1681 gggcgtggac aaggttgatt tcgagctgtg gtcgctggcg gtttcggcga ttaacgggtn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggacaaggt tgatttcgag
     1861 ctgtggtcgc tggcggtttc ggcgattaac gggtgtcagt tctgtgtgaa gtcgcatgaa
     1921 aagaaaatcc gtgagggcgg tttgagcttg gagcaggtgc aaaccgcggt gcggattgcg
     1981 gcggttgtgc aggcggtttc ggctgttctg gacggggctg cggcactggg gggagatgtc
     2041 gcacaggcgg cttaaaagct tgttatcgat gtattttata ggagcgcgcc tgttatgggc
     2101 gcgttttttt tgcattttgt gtttaaattg ggtattttat tacggaaatt aatttgagat
     2161 ggtttttaaa aattgggcag gattgatcaa agtacagtag atacgtcttt cgttcctgaa
     2221 cggcatccgg gggcgcgggc gcgctgggcc atacaaattg cgacggttga tagctttaaa
     2281 tggcgaggga gccacaagct ggccagtttt ttggggcgca atagcgttga gcattctttt
     2341 acgcggatgg ctcaaatttg gtgtgatgaa gatggcaagc cgttgaaaga cccggaaacg
     2401 ggacggcttt atccgcagat ttgcgtgcgc ggggagttaa atcccggagc cattgatcct
     2461 aagacaggcg agtggactgg gctaaggatt gacccgcata ctgttgggaa tattgtttcg
     2521 actttcattc caaggcggct gatggatgct tttaacaatg ccgttttcaa gagtaaaaaa
     2581 tgtaaattgg gtgtttttag ctggtatgga gatggtatag agccgcttga cgccgattat
     2641 gttgtagagg agccggtggc gattagcgat gatcaggatt gtatcgaggc tctgtgggcg
     2701 gtttatcagg agcgtgcgga ggatattaac aggcaaggac ttgattatga tttggtgcgg
     2761 cgaaattgtc atacggtcaa tgcggttttg aatgcaggca atgtcgaggc ggttgagaga
     2821 tttgccaagc ggggttttat gagatggggg gcaaagacgg atgcgattaa ggttgctgaa
     2881 gatacgccgc cggtggtccg gtccttggaa gaactgcaaa agttgaattg caattgggcg
     2941 gatggggttt gtatgagttg tcaaaggctt gctgggcgag gtcagttaca ttctgaggaa
     3001 ctgcaggatg ggtctcatga acttagcgtt gaatatgccg gttagggtgt agttacgggc
     3061 gcgtttttta tttgacaagg cactcatttt tacataattt cacaacggtt aatttttgtc
     3121 ggatttagtg gacgaggttt tacttatgcg ttttggtgtt acaaatgatg ctttgtgtgg
     3181 gctttacgag cgttttggcc ttgatttgga tgatgaacgg gatggtcctg ttcagatgtt
     3241 gtatgaagag acaattactg acgatgatcc tgaaatagga gtgaggcagt ttttgggaaa
     3301 tgtttctctt gataaagtat tgcagtgtca gttgttttct aaggaagatt ataatcgttt
     3361 tctggggccg ccacttggtc ttgccgatta tcttgagcat ttgaagggtg agatgcaaga
     3421 tcatagggaa gatcatacgc gctctattga gattattgac cagcttattc ctcgtacgga
     3481 aagtacggag gatttagttt ttaagggata tttgggacgt tatcaagttt cgtggagcgc
     3541 cagtcccatt atgcgttttg gtacgactgg aatgagtgat gctcctttat cgctcgataa
     3601 gcctcaagac tgtatgaatg cattagagcg tatagaatat gggcacgata gcttttttga
     3661 ggggcgggtg cttgccgttt tgtcgcgatt taagggtgtt gattggccgg gcatccaaat
     3721 tgaggtcgaa ccgtatgagg aggggcctgt gtttgaaccc gaagggaccc ccgtgcccta
     3781 aggttaagcg ccttttttaa cctcgtgcac atagacttcg cggtgggggt aggggatttc
     3841 gatggcgttt tccttgaacg cgtcccataa ggcgagcatg acggcgcctt tgatgttcgt
     3901 tactccggct tcggcgtcct tgatccagaa acgcacagag aagtttatcg agctgtcgcc
     3961 aaattctgtt agatggcata cgggcggcgg atccttgtgg atgcgctcgt gcgcggcggc
     4021 agcgacggct tcggcaattt ctatgacttt atgcgggtcg gagttgtaat gcgttccgaa
     4081 atttacctgc aggcggatga gggagttgcc gtggctccag tttaccacgc gctgggtgat
     4141 gagttcttca ttggggatga ggaaggattt gttgtcacgg gtgacgattt cggtgtagcg
     4201 tgcggccatc tggttgaccc agccgaaggt gccgttttca agttccagca cgtcgccggg
     4261 tttgatggac tggtccatca gcagtagcat gccggaaaac agattcgaga tgcctttttg
     4321 caggccgaaa ccaattccca agccgatggc gccgccgaag acggcgaaca gggagaggtc
     4381 gatgccggct gatgtgacgc cgacgagcag ggcgaagacg atgagggcga cgcggatcac
     4441 tttggctatc agaacctgtg aagagcgggt caggcttttg attctgaata tccggcgttc
     4501 tgcaaagctg gaggcgaata tggccagata cagcaatgtg aatatcgaca ggatgctttt
     4561 tatgacgatg agggcggaga ggcggaaatc tccgatgctg aaggatatgg aatccaatgt
     4621 cgtcatggtt tggtggagca ggccgaagat gctgagcgcg gctatggtcc agatcacgag
     4681 agcgaagaag ttgcggatgg cgctgttggc aatgaactgg acggcggtgc ggatggcaac
     4741 ccaggccagt aaaactttca ttacaccttc ggttagagcg gtgttgacac ccataatatc
     4801 ggatttgagg agttggtcgc cgatcaccag aatgatcaag gcaacgactg gcaggagcag
     4861 gcgcagcagg ttgtttgata tacgccgcgc ggcatacggc atttttgttt tttcgattag
     4921 ttttgcagct ttgctgcgaa acatactgta gctgatgctg gctaagacaa aggcgatgag
     4981 gagtaaaatg ccttgataga gtgtatcggt attccaaaag tgattggcgc accagtttgt
     5041 aatgatattc cagtattctt cgatgttgat attttccagc atggcacaat tctaggcgct
     5101 cttgacgggg ttgaaaagag gcaattgtat gagtacggtt gttgtgagct tgtttaaaag
     5161 cgcttacttt atgcgctgat caggcgcacc gcttctttcg ggtatgtttc gcggaattct
     5221 tcgtttaaat ccgccggacg ggacataagg cggaagaata ttggtccgca cagtaaatcc
     5281 acagccagtt ttgtgtttaa ggatgagcga aattctttgt ggtctttgcc ttgttcgatg
     5341 ctgaattcga cggcatcgat gaggggggta agaaatgttc ttgcgaaaat tttctgagct
     5401 ttgtcatcgg cttgtgcttc agaaaaaagt tgggcgatgg tgtcaccatt gttgccgtcg
     5461 agcatacgga tgagtttatc aagctgcatg ataatggctt cagtattgtt tttcggtgta
     5521 gggagggggc tttggatgcc aggttgagcg cgcagggcgt ccatgacaac ggcggttttg
     5581 ttcggccacc agcgataaat tgtcgttttg ccaactttgg cctttttagc gattgcttcg
     5641 atggagagtt cctgaacgct catgtgcagc agcaaagaat tggtggcctt caaaatggct
     5701 gttttcgatt tttggctgcg cggacggcct ttgcgcgctt gcattgttgg tgtggtttgc
     5761 gcgttgtttt tttgtaatgc agcctgagac atcaatgttt ccctctggtt taaaattgaa
     5821 acagttcgta tcgtatttta aaaattttac tctaggaaac gcagagtggc gactttttga
     5881 ataaaattca agaggagaat gtgtgatttt tacaaaaaaa tgtacatctg tggatgatat
     5941 aaataatgtc agaaaagtct aatattatta cacttatttg tggtgtacgt tattgggtca
     6001 tattctgaaa gctttttggg gcttgatcaa tgacgcccag cctgccgcgg cgaaactcga
     6061 gaacggccag tccgcgttca gctgtgttat cctggcggaa gcggaatata ccgtcaaggc
     6121 cggagaagcc gttcgggttg gttatcgctg tgtggtcaaa ggctggccga cccgttgatt
     6181 gcaggccgcg cctagcaaga atagcggaca gggctgtggc gtcgaaggcc agtgtcgcta
     6241 aacgcggggg gtgggttcca taggtgttta taaaacgttg ttcaaagcta tggcgcgcag
     6301 ccggatcagg cgcggcaaac catgcgcctt ccaggttggc ttcgctggct agtgaggctt
     6361 catcaaacag gccagttcca atacgccgga cactgcgcgg ggggaggtta tagtgggaga
     6421 gcaggctggc gatagcacgt gcatcttgat cgcctacggg catcagtacg gcgtcgaaag
     6481 gcgtttgggc caaggcacca gcgcttttgc gtgcctcata gtgggtaaag gtttttacga
     6541 tgggggcgag gtttgatgtt ccggcagggt agctgatgtt ttcagtaatg gtgatgttat
     6601 agtgtggggc ggcggcgtta aatgcgtttg aaacgatgcg gccataattg gtatcggggg
     6661 caagcacacc gactcgggtt atgccgtgcc ttgctgcgaa gctggtgacg cgtttgacct
     6721 ggtcaaaggg cataaagccc atgacgtagg tgttgctatt ggcaagggtc cagtcggtgg
     6781 aaaaggcgat catattgatg tgtgcgcgtt gggcgacggg ttttgcgctg cgaactgccg
     6841 gggcaaagac cgggccgagg agaagttgag caccggaatc gattgcagac cgggctgcgg
     6901 cttgcgcacc tgcggcagtg ccttttgtat cgcgcgggat gagttcaagg ttggcgtgtc
     6961 cgatttcaaa cagagccatt tgtgcggcct tgagcattga atcgccgagt ttttggtgct
     7021 gaccgcttaa aggaagcagg atgccgactt ttacggtggg caggttaatc atgtggtccg
     7081 tgggtgttat ggggttttgc tggttgccaa ggagttcgtc agaggatgtt gagcttaaga
     7141 tgccgggggc ttgttgggcc ggggcttgtg gaacgcttgt gtgcggtcta tatccactgc
     7201 cggtctggca tgcgccgagc acaagaaggg gcgcgatgag aaagagagca ataaaagcgg
     7261 ttttaaatct ttgtgtcatt gtatccatca tcatttgttt agtatggttt tcaaatgtcc
     7321 gagaaattat cacaaagcga gagtaaagaa aaaaaacgcg gatggctact gccagatgat
     7381 gccgggcggg gtttaacgcc gggattatat ctggtggcga cgccgattgg gaatttgcgc
     7441 gatatatcct tgcgggcgct ggatactctt agccaggcag atggagtttt gtgtgaggat
     7501 acgcgggttt cgggtaagtt gctgaaacat tacgagattg cgcagaagct gagcgtttat
     7561 aatgaccata gtgatgaaac ggtgcgtgcg aagattgtgg cgcggattgg tcgtggtgag
     7621 gcgctggccc tgattagtga tgcggggatg ccgttggtgt ctgaccctgg gtataagctg
     7681 gtggcagacg tgcagcgggc tggtttgaat gtgactagcg taccgggggc gaatgcggtg
     7741 cttgctgggt tgcagctttc ggggctgggt tcggacaggt tttcatttat gggatttttg
     7801 ccgtcgaaaa gcgctgcgcg ccggagcatt ttatgtgagt ggcaaggatc tccggggacg
     7861 ttgattgcgt ttgaaacggc gccacggttg ctggcggcac tggatgatat tgagagtgcg
     7921 ttgccgggtc ggcgtgtggc tgtggtgcgc gaattgacca agctttatga ggaagtgcgt
     7981 tgtgatgatg tggcggcttt gcgcgcgcat tatgaggagc gggggccagt aaagggggag
     8041 attgtgctgg tgattgaggc tgcgcaggtg cgaacgtata gcgaggatga tctgaaggcg
     8101 ttgttggaaa aggcgcttaa agaaatgagt acgagagatg cggcggcgca ggtggctgag
     8161 gtgagcggac agccgaaaaa ggtactttat gcactggcgc tggagatttc gaagtgagca
     8221 ggctaacgaa agaggctgct tatcagcgcg gcgtggcggc tgagcaggcg gctgcacgat
     8281 atttgcagcg acaagggtat aagattttag agcagcgtta taaaacgcgg gttggggaga
     8341 ttgatcttat cgtgcgtcgg ggggagatgc tggtgtttgt cgaggttaag gcgcataaag
     8401 acccggagag cgcgctgtat gcggttacgc cgcgtacgcg tcgacggatt gaaggcgctg
     8461 cggggcaata tctggcggat catgaggagg cgtcgggcct ttccatgcgg tttgatgtga
     8521 ttgtggtgcc gcctgaatct ttaagaaaag ggcttaatct gttgggggca gtttctatac
     8581 gtcatctgga caatgcgtgg ttgtctggtc aataatgtaa gctgcttcgt gtttggtttt
     8641 caggatgatc atcatggttc gtaaatttct gattttatct gtttgtgttt tgtttttgcc
     8701 tggctgttcc gctctgggtg tggcggctgg tgttggcgct actgcgggca ttgccgcagc
     8761 gcaagagggc ggtttgtcgg ctgcggttga tgatgcgcgc attcagattg agattaatga
     8821 tctttggttt aagtatgacg ttgagacatt tcgtaagctg gatctgacga ttaatcaggg
     8881 gcgtgttttg attacggggg ttgtgcaaga tccggaagct cgggtggaag cggtgcggct
     8941 ggcttggcaa cctaaaggtg tgaagcaggt gattaacgag attcaaattg ccgagagtgc
     9001 gggtattgtt ggttttgcca aggatacatg gattacaaca cggcttcgta cggcgattac
     9061 gtttgatcgg gaaatattgt cgattaacta ttcaatcgat acggtgcgcg ggacggttta
     9121 tttgatgggc ttcgctcaaa accgctctga gcttaaccgg gtgattgaaa tagcgcgcac
     9181 gattaacggt gtgaatggtg tggtgagcta tgtgaaattt gttggcggca agacacaaac
     9241 tggtggtgtt gatgatggct caacatatca aagtacgtat gacgataatt atgtttatca
     9301 gaacgcacag ggacaaagtg ttccggtgcg tccgtatgaa gattcaaata tgccgattga
     9361 tttgcgcggg tcacagcctt cgcctgatta tggtacagat tcgtcatctt attcgaataa
     9421 tgttgatccg atgttctctc aggagccgtc tgcgcctgca cgaccaaccc agcgtcaggg
     9481 cattgaaagt gaagaaattc tctggaatga ccagtaatcc tagcggactg gaagagttgt
     9541 ctggtgcatt cgatcctgaa agttatatcc agtctttagg aagcatacct gatgatcgaa
     9601 tcaatctggc gcacgcggct ttggccttgg cagcgcttag tcatgaaggc gtatctttgg
     9661 ggcgctatat ttatcatttg caggtgattg gtgatgaagt gggggcgcgt tatgcagaga
     9721 tttttggtga cagtgctaaa gcgcaattag aggctttgag cgatatattg cacgggcgtt
     9781 atcattatag cggtgatcgt gaaagttatg atgatttaca aaacgccagt ttaattcggg
     9841 tgattgaccg ggccaaaggg cgggcgattg tgctcagtat tctttatatc cacagtgcgc
     9901 gggcgcaagg gtggaatatt gtggggcttg atgtttcagg gcattttctg tgccgtttgg
     9961 aagcgggcgg gcagcgcatt atttttgatc cgtttgaggg tgggcgcatt gttgaggcgc
    10021 aagatatgcg cgaaattgtt aagagggttt ttggggcgca ggcggagctt tcgtcggaat
    10081 attatgagcc ggtggggaat cggggtattc tggttcgttt gcaaaatatt atcaagtttc
    10141 ggcagattga ggctgaggat tatcaaggtg cgctggaaac tgtgcaacgg atgcgacaaa
    10201 ttgatccgca ggaatatcgg ttattgctgg atgaaggggt gcttttggcg cgcacaggtg
    10261 agccgcgagg agctattgat tcgctggttg cctatattga aaaggcgccc aattcgcgtg
    10321 atcggatgga ggctgagctg ttattgcagc agcttaatga tgctctgaat taaagagcgg
    10381 tgaagggttt cgcgtgtatg agcgtatatg tttgtaagga taagttggct ataaaaaatg
    10441 atggctcttg cgcgggtgtt tgtaaccgaa tagaacttga ttctgaacag gcatctgtac
    10501 tgtttatgga gtgtgctcaa tcttgttaag gagaatatta tgaccctctc actgttccgc
    10561 aaagtttcga aggactcttt tgcactgtcc cggctgatga tgattttggc tgttgtgttt
    10621 ttggtttcgg cttgttcatc gaacgatgac gaggaggtta tttttgatgg ccagagtgcg
    10681 gctgcgcgtg agggcattac tgatgggccg ttggatgaaa tttatcaagg tggtgcgcag
    10741 caaggaccgg ctcctggtac gcaggctgat ctggttgtta atgttggtga tcgtattttc
    10801 tttggtacgg atcgttatga tttgaacaat gaagcgcgta tgattttgga taagcaggct
    10861 gcctggcttg agcaatattc gagcttgaat gttacgattg aaggtcatgc agatgagcgc
    10921 ggtacacgtg aatacaacct ggctttgggc gaacgccgtg caaactctgt gcgtaattat
    10981 ctgtctgcgc tgggtattgc tccatcacgc attcaggtca taagttatgg taaagagcgt
    11041 ccggctgttc cgggtgcgaa tgagtctgcg tggtcacaga accgtcgttc tgtcaccaag
    11101 attgactaac aaattgtgat aaatggtctg taaatgacga ttctttcttt ttatatccgc
    11161 aaacggaggg cctggatttt ttcaggcctt ttggctgtgg cgcttgcggg gttaccgttt
    11221 atggcgggtg cgcagggggg tgattgggat atgcaaaacc gcattaaacg gttggaaaat
    11281 gagatccaga cgctcagccg tgcggtctat cgaggtgaga agccgcctgc gggcgttggc
    11341 ggaggcttgt ctggcggggc tgatgccgaa attcgattgc agcagattga aacggaagtg
    11401 cgtggtttga ggggggattt ggaagagcag agctttgaga ttcgtgagct taaagatcag
    11461 cttgagcgtt ttaccagtga tatggaattg cggatgaatg atctggaagg ttctggagct
    11521 tcgcgttccg gcagcgctgc gccggtgagt ggggccagtc gttatacggc taggccgctt
    11581 agagatagca atccaccgag cgttgttggt agtggcgtcg ggcagactta tcagtggagt
    11641 tccagtaatg ccaacagtgg aggtgcggtc gggcagcttg gttcgtatac ggaatcgggg
    11701 gacgggacat cgtcttctga tttagcgtct gcaacttatg aaaatgcatt ttcgatggtg
    11761 aaaaatcatc aatataaggg ggcgcaggtc gaatttgaga gtttcttgcg tgatcaccct
    11821 gatcatccgt tggcgggtaa tgctaaatat tggctcggcg agacacatta tgtacgcggg
    11881 aattttgaaa cggctgcgcg gatttttgct gaggggtatc agcaatatcc gaaaggttct
    11941 aaagcggcgg ataacttgct gaaactgggg ttatctttgg atgcgctggg gaagaaggat
    12001 gatgcatgcg tggcgcttcg tcagcttaag aaagaaaatc cgtccggggc tgcgcccgtg
    12061 atgcgtcggg ccgatcagga aatgtcccgg atcgggtgtt aaagagctgc tatcgtttag
    12121 ctgtttgctc tttggaagct ggtgaaatct tttgaatttt gaagacttta atcatcgtat
    12181 gttggcgctg ggtgtggggg gcactgggcc gattgctgtg gctgtgtcag gtgggccgga
    12241 ctctatggcg ctgtgctggt tgttgtcgcg ctgggcggat ggacgcgggg tttatgttca
    12301 tgctttgacg gttgatcacg ggctcaggcc tgaagcggct gatgaggctg tacaggtagg
    12361 cgcgtggttg gatggctggt cgcagcaggt ggggcatcat attttgcgct gggagactcc
    12421 agcggacaca gccattcagg aagaggcgcg cagggcacgt tatggtctga tggctgagta
    12481 ttgtgagtgt catgatattg agtatttgtt tttggcacat caccgggatg atcaggcgga
    12541 aacggtgttg tttcgtttgg ccaaaggcag tgggctggat gggctttccg ggatgcagga
    12601 gtgtcaggct tatgatgagc gtttaacgtt agtgcggcct ttgttggatt gtggtaaggc
    12661 ggagttattg aaattatgcg gggcggaaga tatttcatat tttgaggacc ccagtaatgc
    12721 aaatagtgat tttgcgcggg tacgattgcg taaggcgcgg tgtgttttgg aggaagaggg
    12781 acttacttcg aagcgattgg cggtaacggc acagcgccta gggcgcgcgc gccgtgcttt
    12841 ggaggttatc agcgcgcagg cctatcaaaa tgcggccatt gaatcaaata cgaaacatat
    12901 cgtatttaaa attaatattt tatttatgca gcctgaagag gttattttgc gatgtttgtt
    12961 aaaggcgttg tcggttttgc gtccgctatc cgattatgcg ccgcgtatgg aaagggttga
    13021 ggtgatttta tatgatttga tggctgttaa aaataagggc acgccgtttc gaaagcgcac
    13081 attggggggg gtgatgtttg caatggacga gaatttgggg attttgcgtt tgacgcgcga
    13141 gtattcttaa gaaccctttc gaaaaagtct gggaaagaaa aaaagaggcg ttttttatat
    13201 ggttcttaat catgattgtt tgaaaaacca cggttttagc cttgattttc cttgtctgga
    13261 gcggaaattg tactacattg gatattctga attagaagaa ggataggcaa ctgtgaatgg
    13321 atttggtcgc aatttgattt tctggctggc gattggcctg accatgattt tcctgttcaa
    13381 tatttttcag ggcgcgcagc aaggggcaat taaccgcggg aataagattt cctatagtga
    13441 atttatgacg gatgcgcagg cggggcgtgt ttcgaatgtc gttattaaag ggcaggaatt
    13501 gcacggcgta tttgcttcaa gcggcgaaga attcaaggtg atggtgccgc cgaa
//
LOCUS       DAOSWR010000049        13316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2955_length_13316_cov_5.710429, whole genome shotgun sequence.
ACCESSION   DAOSWR010000049 DAOSWR010000000
VERSION     DAOSWR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2955_length_13316_cov_5.710429"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="pyrB"
                     /locus_tag="PK513_05130"
     CDS             complement(<1..306)
                     /gene="pyrB"
                     /locus_tag="PK513_05130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOO81863.1"
                     /translation="MRHLLGIDELDAEQIAVFLSRAQEYSDALKSGSWDTERLKNKVI
                     LHLFFENSTRTLTSFEIAAKRLGADVVNWNPETSSLAKGENFIDTVDTLVSMKPDAIV
                     "
     gene            548..868
                     /locus_tag="PK513_05135"
     CDS             548..868
                     /locus_tag="PK513_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81864.1"
                     /translation="MPVETQTDPKRGIIHKDPLLAFEDGKLVMVVEHHFENVPSWVEW
                     DKDRQIVTITQMNGDLDEAKLEIKEDCIGRLEMARNLLLVSLDVSNDNDERIMHSVPI
                     LIRT"
     gene            901..1086
                     /locus_tag="PK513_05140"
     CDS             901..1086
                     /locus_tag="PK513_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81865.1"
                     /translation="MEILNKNEASFAGYEAIVAAAVAIENPQEGETYNLGVIKVATSN
                     TVASVGLQNEPDGPKLN"
     gene            complement(1079..2305)
                     /locus_tag="PK513_05145"
     CDS             complement(1079..2305)
                     /locus_tag="PK513_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOO81866.1"
                     /translation="MNKALHNIRILDLSRVLAGPYCTQMLGDLGAEILKVEKPGSGDD
                     TRKWGPPFLQNGQGSNTSESAYYLSCNRNKKSIAIDLTTPKGQEIVKSLIAQCDIVIE
                     NFKVGGLKKYGLDYESLKKDHPALIYCSITGFGQTGPLAREPGYDFLAQAMTGLMACT
                     GDPDGAPMKVGVALGDVMTGLNAAVGILAALYHRRETGQGQYIDLALGDCTLAALTNI
                     AQYYLTSGRPAPRVGNAHSTIVPYQAFKAADGYVILAVGNNEQFQRFCDFAGHSEWAE
                     DERFATNSARVLHRETLVPMIETLIARHDIAHWIDGLHDADVPCGPVNTMDKVFGDPQ
                     ISARDMKIEMEHPLTNAPVHLVGSPLKLSGTPVTYKYAPPVCGQHTREILSQQLRLSD
                     AEIDALAAEQIIQIVS"
     gene            complement(2302..2814)
                     /locus_tag="PK513_05150"
     CDS             complement(2302..2814)
                     /locus_tag="PK513_05150"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOO81867.1"
                     /translation="MLEIVSILCLLIGTGLLAALIIIDLRTLLLPNILVLSLALAGFM
                     FHASTLFFYMPVSDMVLGGFIGGGTLYLIRALGNAFYKTDTLGLGDVKLMAVGGLWLG
                     SYFILIALMAGAVAGLVHGLMLIVRQWIKTRRIESLSTFSLPAGPGFAAGLFIAALEK
                     FFPLTHLGLS"
     gene            3031..3417
                     /locus_tag="PK513_05155"
     CDS             3031..3417
                     /locus_tag="PK513_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO81868.1"
                     /translation="MTESAKILVVEDNDFVRMQIAKFLQDEGYEVIEGTDGSDALEKM
                     APDVAMAIVDVRMEPIDGFEFIKTLRSEDNETPVVLVTGDQNPDLLAEAGKWHVAAVL
                     MKPVQRDRLVKTVSRTLQSLSRASAQ"
     gene            3414..4976
                     /locus_tag="PK513_05160"
     CDS             3414..4976
                     /locus_tag="PK513_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system family protein"
                     /protein_id="HOO81869.1"
                     /translation="MTDGKTNKVSMKRFCSRSSMLLVLGAVSLVFLGSCADVNTPGAR
                     NANIPSPAMADPRPEAINEQPDSVIYLPLGSDVLVPEVIRGAALPDEVVGPFELRSET
                     LAGALQLILADYQIPLAFETDEGLTRTITVANLRGPLSNVVDKVCGLADLYCSFEDGL
                     LVIKDMQTFTVSIPPIGGNTDMLSSLATGLQAITGKTPITETATRTIIYEATSRTAQQ
                     AERYFQRIRSNTALIVFEVYIWEVGLNSDNATGIDWQKIDVFGKYATGINIPGGVGAG
                     FTPISIGLPTVTGTPSFDGNDVLEFISNYGSVKTISQPQITVLSGSEATLRAADTVNY
                     VSSLSRSVDNGEVTVSTETDSVDTGFTLTISSAWDKATIYGNIDIELQEFRRFQNFDA
                     DGTTLQLPETTERELSTQIRIRPGDSLLIAGLVRENDQYDTAGPGFNKPLFPTSRSAT
                     VSNTELVFLLKPRVIVYTSEGKAENYAQKTIVQQGNGVFGQDSVSSEQLPQPIIFPTS
                     TIPADLLNPSGH"
     gene            5107..6774
                     /locus_tag="PK513_05165"
     CDS             5107..6774
                     /locus_tag="PK513_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOO81870.1"
                     /translation="MAVLYFDQIFIIIMRLHLFILMASMLCFSVFISGNALAQYPGFD
                     DPGKTVSGGGSAGLVPVTPSIDGGAIPIGATAQVVVLFRNEGGQPVETGTIRLYPSST
                     VSAIVSLNQCENEPLPSGAECAIALSVKGLQAGPWRVEMLMAHSGRTRLVTATVMGSV
                     ETTEGAGDKLTSDIEMIPDTVDFGSLNAAQTLIEPVILRNITSNEISINDIYIDTSEQ
                     AGYALKTECSKLAAGQSCIATITWSPKLKGRSSGVLVVKHTGPAALSSVPLKGEYAPE
                     NVSSAQVFPQAVPGKGLLVSSAEDIDFGSGIETASSITVSLVNSGDADLMLRDIRISG
                     SDNGVGFKEDGCLPGTVLEPVEACPLTVTWSPTRVGDILDDIQILHDGARGVLVLPVR
                     GNALSTVSQDQKAIVLSEGAIELDGEAPERSEEALEAGVPETQEEAAQKNAARVGAKP
                     SYAASSSANISGALDGYKITSFSPTRAIINGPGGSRLVFDNEEVVLGGVPWMVLIQPS
                     GIEFMHRGKRVLLLFDRSLSSINRVNASSSESGSSSGSSSSSDASDG"
     gene            6777..8546
                     /locus_tag="PK513_05170"
     CDS             6777..8546
                     /locus_tag="PK513_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO81871.1"
                     /translation="MDDTTQNDDKRKQGGDGPPQGVERRKADSRKRMRSSEGRERYLD
                     MVQRERALLLDQGIARSTEQLLDMAGAAVPSDIEDEGVKDRATGDQLRAILPVQSWLP
                     LSIHLIGLRDGIMRIAPLHDMNDRQINSLISTAQRSGFHVEKIEIEVWDRKELIDTLR
                     SVHDLSADRCEKTLALWLTDPDNGLLLNQFQRDMMAEALQMRASDIHLVQDNNPDAPN
                     WIQYRIDGDMIPMHLLPADAMARLTTLLKRDAGLNFGDRTTPKDGRFGFTWQGRAIDV
                     RVAAGPQAQDGEKLTLRLLDRAALKGFDELFSRHEDVADYLAQLLSPDIKGGGGMILL
                     SGPTGSGKTTTLYACVQQIDRRRRHVLTIEDPIEYELRYATQWQVRPGMEGGGFADLI
                     RASMRHDPDYIIIGEMRDADTVETSLKAAESGHTVISTIHADTALQTFERLRSLMPSE
                     RERSSTYTLAQQVKCILNQRLVRTLCQGCAHKEEAGKVLKEHELKLFDLAPEARVRRH
                     NTSGCDLCNRTGISGRTLLLDALLITLGPAHRNEIYEALIGNVNNVVNEEGVVVHTRR
                     EGLIDLVLSGLVDPKAAISYLEQ"
     gene            8739..9959
                     /locus_tag="PK513_05175"
     CDS             8739..9959
                     /locus_tag="PK513_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO81872.1"
                     /translation="MQQWVAEIAYETPSGPKKVSESFYLPSVDDVRVAVTKKGGFVLS
                     IRPHARSPLERLLARSTWWQVQLLRGIQFRSTSTSPGVALWKIIQAETNPRRQNIMAP
                     AREALARGLGVIDALKALNTFDHGTLAILAGSERANRLVEGIPHAIHSITQKKKNAAA
                     IMGTMGWLAFDVFSIVSSLWAGKDMVLGWFRDNTPTDPEELEKFLTVVGRLELTWDVL
                     IFTSVGFGAFMAWCVFSYWINRGKRDFPTARIVRKIPLIGGYLRDLAFADSMSAAARM
                     LRGLVPINDTLDQSSEASSAPDVTEYWKESLKDLGRGVSLGAALDRAPLTRSERLEVA
                     GLSDLGQVATVMESIAEMRSQAARTKHKLIVWIAFLLTGIFLAIAFGSAIYALTVMNM
                     SMDSMMGGLMEGAI"
     gene            9970..10953
                     /locus_tag="PK513_05180"
     CDS             9970..10953
                     /locus_tag="PK513_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81873.1"
                     /translation="MALISKEDKGLPGKRIQENASGVSRAAPGISDYLAGEAASVPTD
                     LKPYLPQELVGGVIPHIPGTEEEAVWNAASQACGTEKVHYCYSVDENKIWYLACPSAS
                     LASNPDSWCPLAAALPGNSEYWDRETVYLYEQEGLASALRWDPETGRMQVFLGAARTI
                     LPRVQSMDANFVTINPDVADLVPWKNRMLKTEKLSRATVRMLLFIGMIVNFIAIIILL
                     ISYVSVNMIERNLSKVQEETQLASNQLVQQAKDAMQSDTIKHMVRVQEILDGLGRLDS
                     TLVRYEIKGAQVEWQALVPPAYIQTVMTKYPVRVDDTVKEKDGRIRVIGNR"
     gene            11078..11620
                     /locus_tag="PK513_05185"
     CDS             11078..11620
                     /locus_tag="PK513_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81874.1"
                     /translation="MDFSKLQQSFKEFDWRSLKKYTSPQAADDLNAFLEKMPQNAGQT
                     MLIIAGLMWAMGGAIGLFTTVQLQKLTETRAQLQEAQALKPSVPQVRDVPVNPNEVDE
                     FVTKVKDIYTGLKISASGPSISITATTLGSFGQFREAIGHVQNGGAGWNVNIQNFCVG
                     RECERYPLSASLKINKVSVK"
     gene            11663..12460
                     /locus_tag="PK513_05190"
     CDS             11663..12460
                     /locus_tag="PK513_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81875.1"
                     /translation="MLKTLKRFYKNKKTLGRAAERGNVLFLILIAVALFAALSYAVTQ
                     SSRSGGGASDGESNLVNSAQITQYPASVRTAIVRMIIGGTDVGQLNFDPPSAFSIAVT
                     GCDVASVSSTCVFHPDGGGATRVTAPPEVMASGSQGDWIFTSDFAIENIGRTTASNNS
                     NDVIAFLPGVGQSLCERLNTELGVPTSTDADGDSVPDAGIVIGNVPTAADEMDNNNLG
                     IGPAAAGAGKRLTNDLSGQPFGCADFDATTNQADDSDLVYFHVLVER"
     gene            12469..>13316
                     /locus_tag="PK513_05195"
     CDS             12469..>13316
                     /locus_tag="PK513_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81876.1"
                     /translation="MNLQPYKIVTKRASEAGSALVYILIAIALLAALTISFMEPSSQQ
                     TSSQNTFKTVSALQGQIDIIRSAIQECVLIYPNGDPTINTAPAGSDPNPYVKFPINPD
                     STHYTSATPGRSGDRLVRNMRCPGNPTTANVYDHGLIFTGSSGKFLGPAPDLFDDWQI
                     YNGEDGVFYWTETDKTDAFVKAALEKLDEKFSECEADVIDTSDAPAGAKDLDSNALLT
                     CPANHLCFRVWIIQNEATNVYNGDAAGDEGGGSCHGFHEINPFRLSAVCPAGEVSPHE
                     EEGHVG"
BASE COUNT         3312 a         3177 c         3489 g         3338 t
ORIGIN      
        1 cacgatcgca tccggcttca tcgacaccag cgtgtcgacc gtgtcgataa aattttcacc
       61 cttggccagc gagctggtct ccgggttcca gttcaccaca tccgcgccca gacgcttggc
      121 cgcaatctcg aatgaggtca gcgtccgcgt cgaattttca aaaaacagat gcaggatcac
      181 cttgttcttc agccgttccg tatcccacga tccggactta agcgcatcgg aatattcctg
      241 cgcccggctc aaaaacacag cgatttgctc ggcatccagc tcatcaatcc ctaataaatg
      301 gcgcataatt gtatccccgt atgaaaaaaa cacatcacat aaagcaaaac catagaacct
      361 tatccgatgt ccagagggaa aatttttgct atactcagga aaggcaattc tgtcgctttt
      421 cttctttttt ctctttttct ttcattcaaa aggcaaaaaa atgttcaata ttataagaat
      481 attaatagat ttcctgcata aaatagggtt aagctttggg acaaaatcag agcaaagtgc
      541 acggcccatg ccggttgaga cgcaaacgga cccaaaacgc gggatcattc ataaagatcc
      601 gcttttggcg tttgaagacg gcaaactggt catggtggtc gagcaccact ttgaaaatgt
      661 cccgagctgg gttgaatggg ataaggacag gcagattgtc accattaccc aaatgaatgg
      721 tgaccttgat gaagccaagc tggaaatcaa ggaagattgc atcgggcgtt tggaaatggc
      781 acgcaacttg cttttggtaa gtctcgatgt cagtaacgat aatgatgaaa ggattatgca
      841 ctctgttccc atccttataa ggacgtaaaa acaggcaggg tgtgttaaag gagaaaagag
      901 atggaaattt taaacaaaaa cgaagcaagc tttgccgggt atgaagccat tgtcgccgcc
      961 gccgttgcga ttgaaaaccc gcaagaaggt gagacctata atcttggcgt aatcaaggtt
     1021 gccaccagta atactgtggc atcagtaggc cttcaaaatg agccggacgg cccgaaactt
     1081 aactaacaat ctggataatc tgttcagccg ccagcgcatc aatctccgca tcggagagcc
     1141 tgagctgctg gcttaaaatc tcgcgcgtat gctgcccgca tacgggagga gcatatttgt
     1201 aagtgaccgg cgttcctgag agcttcagcg gactgccgac cagatgaacg ggcgcattcg
     1261 ttagcgggtg ctccatttca atcttcatat cgcgcgcact gatctgcgga tctccaaaaa
     1321 ccttatccat agtattgacc ggcccgcagg gcacatcggc atcatgcagt ccgtcaatcc
     1381 agtgcgcaat atcatgccgg gcaatcagcg tttcaatcat cggtacaaga gtttcacgat
     1441 ggagaacccg cgcgctgttg gtggcaaagc gttcatcttc agcccattcg ctatgcccgg
     1501 caaaatcgca aaatcgttgg aactgctcat tattgccgac cgccaaaatg acgtaaccat
     1561 ccgcggcttt gaaagcctga tacggcacaa tcgtcgaatg cgcattgccc acgcgcgggg
     1621 cagggcggcc tgaagtaaga taatattgag caatattggt tagtgccgcc aaagtgcaat
     1681 cgcccagcgc cagatcgata tattgtccct ggcctgtttc acggcgatga taaagcgccg
     1741 ccagaatgcc caccgccgcg ttcagccccg tcataacatc acccaatgcg acgccgactt
     1801 tcatcggggc gccgtcagga tcgcccgtac aggccatcaa tcccgtcatc gcctgcgcaa
     1861 gaaaatcgta tcccggctca cgggccagcg gccccgtctg gccaaacccg gtaatcgaac
     1921 aatatataag ggccggatgg tcttttttca ggctctcgta atccagcccg tatttcttca
     1981 gccctccgac cttgaaattc tcaatcacaa tgtcgcactg cgcaatcagg gatttaacga
     2041 tctcttgtcc tttgggtgtg gtcaaatcaa tcgcaatcga ttttttattc cggttgcacg
     2101 acaggtaata ggcgctctcg cttgtattgc taccttggcc gttctgtaag aaaggtggtc
     2161 cccatttacg cgtgtcatcg ccgcttccgg gtttttcgac cttaagtatc tccgcgccaa
     2221 gatccccaag catttgtgtg caatacggcc ccgccagaac gcgggaaaga tcgagaatgc
     2281 gaatattgtg aagcgcttta ttcatgatag accaagatgg gtaagaggaa agaatttttc
     2341 aagagccgcg ataaacagcc cggcggcaaa tcctggtccg gcgggtagcg aaaatgttga
     2401 gagcgattca atcctgcgcg ttttgatcca ttgccgcacg attagcataa gcccatgcac
     2461 aagcccggca acagctccgg ccatcaaggc tatcagtatg aaatacgagc ccagccacaa
     2521 cccacccacc gccatcagct tcacatcgcc tagccccagc gtatcggttt tataaaacgc
     2581 attgccaaga gcgcgaatca gataaagcgt tccaccgcca ataaacccgc ccagtaccat
     2641 atcgctcacc ggcatataga aaaacagcgt actggcatga aacataaatc cggccagtgc
     2701 gaggctcagc accagaatat tcggcagcaa caaggtgcgc agatcaatga taatcagggc
     2761 ggcgagaagc cccgtgccga tcaacagaca aagaatggaa acgatttcga gcatagatac
     2821 caggccgctg cgtgaaacag attgcaacag tctaacagtt tttatcaggc ggctaaatga
     2881 gttttgaccc taaaactgtg tacagtcctg ataagggttc tgttctgatg cgttattttg
     2941 ctttaaagtg attcgaaacg tgtatttttc aaacactctt gacgtgtggt tttgtcctaa
     3001 ccttttattt attctaactt ttagagttct atgactgaat ctgccaaaat tttagttgtt
     3061 gaagacaatg actttgtccg catgcaaatt gccaaatttt tgcaggatga aggctatgaa
     3121 gttatcgaag gaacagacgg aagcgatgcg cttgagaaaa tggcacccga tgttgcaatg
     3181 gccattgtgg atgtgcgcat ggaaccgatt gatggttttg agtttattaa gactctgcgg
     3241 agtgaggata atgagacgcc cgttgttttg gtgacggggg atcagaatcc cgatcttttg
     3301 gctgaggctg ggaagtggca cgttgctgca gtcttaatga aaccggttca gcgcgacagg
     3361 cttgttaaaa cggtgtccag aacgcttcaa tctttatcgc gggcaagtgc gcaatgacgg
     3421 atggtaaaac gaataaggtc agtatgaaac gtttttgcag ccgaagttcg atgttgcttg
     3481 tgctcggtgc tgtgtctctg gtgtttttag gaagctgcgc cgatgtgaat acgccaggtg
     3541 cgcgtaacgc aaacattccg tctccggcaa tggccgatcc tcggcctgag gcgattaacg
     3601 agcagcccga tagtgtgatt tacctgccgc taggaagtga tgttctcgtg cctgaggtta
     3661 ttcgtggagc ggccttacct gatgaggttg ttggtccgtt tgagctacgc tcagaaactc
     3721 ttgccggagc tttacagctc attctggctg attatcaaat tccgctggct ttcgaaacag
     3781 acgaggggtt aacgcggaca atcacggtgg ccaatttacg cgggccgctc agcaatgttg
     3841 ttgataaggt ctgtggtctt gcggatctgt attgttcttt tgaggatggc ttgctggtca
     3901 ttaaggacat gcagacattc acagtctcta tcccgccgat tggtggaaat acagatatgt
     3961 tatcatcgct tgcaacgggc ttgcaggcga ttacaggaaa gacgcccatc acagaaacgg
     4021 caacgcgcac aatcatttat gaggcgacaa gccgtaccgc ccagcaggcg gagcgctatt
     4081 ttcagcgtat tcgttccaat acggccctga ttgtttttga agtttatatc tgggaagtgg
     4141 gtctgaattc tgataacgca accggtatcg actggcagaa aattgacgtc tttggtaaat
     4201 atgcaaccgg aattaatatt cctggcggtg tcggcgcagg ctttacgcca atcagtatag
     4261 gcttgccaac agtcaccgga acgccgtcct ttgatggcaa tgatgtgctt gagtttattt
     4321 ccaattacgg gtcggtgaaa actatatctc agccgcaaat cacggttttg tctggttcgg
     4381 aggcaacgct gcgcgctgct gatacagtga actatgtctc ctctcttagt cgttctgtcg
     4441 ataatgggga ggtgacagtt tcgacggaaa ccgattcggt agacacgggc tttacactga
     4501 ccatttccag tgcctgggac aaagctacaa tttacggtaa tattgatatt gagctgcagg
     4561 aatttcgccg ctttcagaat tttgatgccg atggcaccac gctgcaattg ccggaaacaa
     4621 ccgagcgaga actttcaacg cagattcgca ttcgtcccgg tgattcgttg ctgattgccg
     4681 gtttggtacg agagaatgac caatatgata ccgctggccc gggttttaac aagccgctct
     4741 tcccaacatc gcgttcggca actgtgtcca ataccgaatt ggtcttcctg ctgaagccgc
     4801 gcgtgatagt gtatacatca gaagggaagg ctgaaaacta cgcgcaaaaa acgattgtgc
     4861 agcaaggtaa tggtgtgttt ggacaggatt ccgtttcatc tgaacagttg ccccaaccaa
     4921 taatctttcc aacaagcacg attcctgctg atttgctgaa cccatccggg cattaatctt
     4981 tgggttgaag atgcgttttt atccggggat gctaaaactg aataaaagtc gaaaaaacgg
     5041 tatttaatgc tgtttcagtg aatttttatg ttatgattca acatcagtca tgcgtgtgag
     5101 tacgctatgg cagtccttta ttttgatcag atattcatca ttattatgcg tttgcatctt
     5161 tttattttaa tggcttcaat gttgtgtttc tcggtattta tatcggggaa tgcgctggcg
     5221 caatatccgg gatttgatga ccccggtaag acggtatcgg gtggggggtc ggcaggcttg
     5281 gtgccggtta ccccaagtat agatggtggg gccattccaa ttggagcgac agctcaggtt
     5341 gtggtgctgt tccggaatga gggcggtcag cccgttgaaa ccggcacaat ccgtctttat
     5401 ccctcttcaa cggtttctgc aattgtttct ctgaatcagt gcgagaatga gccgcttcct
     5461 tctggcgccg aatgtgcaat cgccttgtct gtaaaaggtt tgcaggccgg gccttggcgt
     5521 gttgaaatgc tgatggcgca cagtggccgt acgcgcctgg tcacggcaac ggtgatgggg
     5581 agcgtggaga caaccgaagg cgctggagat aagcttacaa gcgatattga aatgatccct
     5641 gacacagtcg attttggatc tctcaatgcg gcgcaaactc ttatcgagcc tgtgattttg
     5701 cgcaatatca cctcaaacga gattagcatt aatgacatct atattgatac cagcgaacaa
     5761 gccggttacg cccttaaaac cgagtgtagt aaattggccg ccgggcaatc ctgtatcgcg
     5821 acaattacgt ggtccccaaa gctaaaaggg cgctcttcag gtgtattggt ggttaagcat
     5881 acggggcctg cagccctttc cagcgtaccg ttgaagggcg agtatgcccc ggaaaatgtg
     5941 agcagtgcac aggttttccc tcaggctgtc ccgggtaaag ggttgttggt ttcctcggcg
     6001 gaggacattg attttggctc ggggattgaa actgcttcaa gcattactgt ctcgctggtg
     6061 aattccggcg atgctgattt gatgctacgc gatattcgta tttctggctc tgataacggt
     6121 gtagggttta aggaagacgg gtgtctgccc ggtacggtgc ttgagcctgt cgaggcttgc
     6181 ccgttaacag tgacatggtc tccaacccgc gtgggcgata ttctggatga tattcaaata
     6241 ttgcacgatg gcgcgcgcgg tgtgttggtg ttgccggtgc gtggtaatgc cctttcaacg
     6301 gtaagtcagg accaaaaagc aattgtttta agtgaagggg cgattgagct tgatggcgaa
     6361 gcgcccgaac gttccgaaga agcccttgag gcgggtgtgc cggaaacgca agaagaagct
     6421 gcgcagaaaa acgctgcgcg tgtcggggct aagccgtcct acgccgcatc atcgtctgca
     6481 aatatctccg gcgctttgga cggatataag atcacctcat tttctccaac gcgcgccatt
     6541 attaacggtc cgggcggaag tcgccttgtt ttcgataatg aagaagtcgt tttgggcgga
     6601 gtgccttgga tggtactgat tcagcccagc ggcattgaat ttatgcatag aggaaagcgc
     6661 gttttgctgc tttttgacag atctttatct tctattaacc gtgttaatgc atcatcaagc
     6721 gagagtggga gcagttctgg atcttcctca tcaagtgatg caagtgatgg ttaaatatgg
     6781 acgatacaac gcaaaatgat gataagcgca aacaaggcgg ggacggccca ccacaaggtg
     6841 tagagcgccg taaggccgat tctcgtaagc gtatgcgctc cagtgagggg cgtgaacgct
     6901 atctggatat ggttcagcgc gaacgcgctt tgttgctcga tcaggggatt gcccgctcta
     6961 ccgaacagct gttggatatg gcaggcgctg ccgtgccgtc cgatattgaa gacgaaggtg
     7021 ttaaagaccg cgccaccggc gaccagcttc gtgcaatcct gcctgttcaa tcctggctac
     7081 ctttaagtat ccacctgatc ggcctgcgtg atggcatcat gcgcattgca ccgttgcacg
     7141 acatgaatga tcgccagatc aattctctaa tctcaaccgc gcaacgaagc ggtttccacg
     7201 ttgaaaagat tgaaatcgag gtttgggacc gtaaagaact tatcgacacg ctacgatctg
     7261 tgcacgattt gtctgccgat cgttgcgaaa aaacattggc gctgtggctg acagaccccg
     7321 ataatggctt gcttttgaac cagtttcagc gcgatatgat ggccgaagca ctgcaaatgc
     7381 gcgcctccga tattcacctg gtacaagata acaaccccga tgcgccaaac tggattcaat
     7441 atcgcatcga tggcgatatg attccgatgc atctgcttcc tgctgatgca atggcgcgtc
     7501 tgacaacctt gctgaaacgc gatgcgggcc tgaacttcgg ggacagaacg acgcccaaag
     7561 atggccgctt tggctttacc tggcagggcc gagcgattga cgtgcgtgtg gcggccggtc
     7621 ctcaggcgca agacggtgaa aagctgacat tgcgtttgct tgaccgcgcc gcgctcaaag
     7681 gttttgacga attattcagc cgtcatgaag acgtggcgga ctatttagct caacttctct
     7741 cgcccgatat taagggtggc ggggggatga ttttgctctc cggtccgact ggttctggta
     7801 aaaccacaac actctacgct tgtgtgcagc agatcgaccg ccgccgtcgc cacgttctga
     7861 ccatcgaaga tccgattgaa tacgaactgc gttatgcaac gcaatggcag gtccgtcctg
     7921 gtatggaagg cggggggttt gccgatctga ttcgcgcctc aatgcgtcat gatccggact
     7981 atattatcat cggggagatg cgtgatgctg atacggtgga aacgtctctt aaggccgctg
     8041 aatccggtca caccgtgatt tcaaccattc acgccgacac cgcactgcaa acctttgagc
     8101 gcttgcgctc tttgatgcca tccgaacgcg agcgctcctc gacctataca ttggcccagc
     8161 aggttaagtg cattttgaat cagcgtttgg tccgcacgct ttgccaaggt tgtgcacata
     8221 aagaggaagc gggtaaggta ctaaaagaac atgaattaaa actcttcgat ctcgctccgg
     8281 aagcaagggt gcgccgtcac aatacttcgg ggtgcgattt atgtaaccgc accggtattt
     8341 cagggcgaac gctgcttttg gatgcgcttt tgattacatt gggcccggcg caccgtaatg
     8401 aaatttacga agctttaatc ggaaacgtga ataatgtagt gaacgaggaa ggtgttgtag
     8461 tgcacacgag gcgcgaggga ctcattgacc tcgttctgag cggtcttgtc gatccgaaag
     8521 ccgcaatatc ctaccttgag cagtaataac ccctatgtca tcccggcgca ggccgggatc
     8581 tcataccggt aacagcaaga ttcctgcttt cgcaggaatg acaggtaaag aatgttttgt
     8641 cgcttcgctt ttcttttaca caaggcagga gaagcgatgc gtgtaattga tacgcgagcg
     8701 acgagtaacg ctgtgtaaaa gaaaagcgaa gtgacaaaat gcagcaatgg gttgcggaaa
     8761 ttgcatatga aacgccgtcg gggccgaaaa aggtcagcga atcgttctac ctgccatctg
     8821 ttgatgatgt tcgcgtcgcc gtaaccaaaa aaggtggctt tgtgctgagc attcgcccgc
     8881 acgcacgttc gcctttggaa cgcctgctgg cacgttcgac ctggtggcag gttcagcttt
     8941 tgcgcggtat tcagtttcgc tcaacatcaa catcaccagg cgttgctcta tggaagatta
     9001 tccaggctga aacaaacccg cgcaggcaaa atataatggc cccggcccgc gaagccttgg
     9061 ccagagggct gggcgtaatt gacgcgctca aagcgctcaa tactttcgat catggaacat
     9121 tggcgattct tgccggttcg gaaagagcta accgcttggt agaggggatt ccacacgcca
     9181 tccactccat tactcaaaag aagaaaaacg ccgccgccat catgggaacc atgggttggc
     9241 tggctttcga tgtgttttca attgtgtcat ccttgtgggc tggtaaagac atggttttag
     9301 ggtggtttcg cgataatacg ccaactgacc ccgaggaact tgaaaaattc ctgactgttg
     9361 ttgggcgtct ggaactaacc tgggacgttt tgattttcac atctgtaggg tttggagcct
     9421 ttatggcatg gtgcgtgttt tcctattgga taaaccgcgg aaaaagggac tttccaaccg
     9481 cgcgcattgt ccgcaaaatc ccgcttattg gcggctatct gcgcgatttg gctttcgctg
     9541 attcaatgtc ggcagccgcc cgtatgctgc gtggactggt gccgatcaac gatacgcttg
     9601 atcaatcttc agaagcttca tccgcaccgg atgtaacaga gtattggaag gagtcgttaa
     9661 aggaccttgg gcgcggggtt tcattgggag ccgccttgga ccgcgcgccg ttaactcgca
     9721 gtgaacgtct tgaagttgca ggcttgtctg atctcggcca agttgctaca gtaatggagt
     9781 ctatcgccga aatgcgttcg caggcggcgc ggacaaaaca caagttgatc gtttggatcg
     9841 catttcttct aaccggcata ttccttgcca tcgctttcgg gagcgccatt tacgcattga
     9901 cagttatgaa tatgagtatg gattcaatga tgggcggtct gatggaaggc gcaatttagg
     9961 gaaaaagatt tggctctaat ttctaaagaa gataaaggct tgccaggtaa acgcattcag
    10021 gagaatgcgt ccggcgtttc gcgcgcggcg cccggtataa gcgattatct ggccggtgaa
    10081 gccgcaagtg tacccacaga cttgaagcct tacttgcctc aggaactcgt cggcggcgtt
    10141 attccccata tccccggcac ggaagaagag gccgtctgga atgcagcgtc gcaagcctgt
    10201 gggacggaaa aggtgcatta ttgctattcc gtcgatgaaa ataaaatctg gtatttagcc
    10261 tgcccgtctg catcattggc ctcaaaccct gacagctggt gccctctggc cgccgcgctt
    10321 ccgggaaata gcgaatattg ggatcgtgaa acggtctatc tctatgaaca agaaggacta
    10381 gcctcagccc tgcgctggga cccggaaact ggccgcatgc aggtgttttt gggggctgcc
    10441 agaacgattt taccgcgcgt acaaagcatg gatgcgaatt tcgtaacgat taatccggat
    10501 gtggcagatc tggtgccatg gaaaaatcgt atgcttaaaa cagaaaaact ttctcgcgct
    10561 acggtacgaa tgttgctgtt tattggcatg atagtgaatt ttatagcaat tataatcctg
    10621 ctgattagct acgtatcagt caatatgata gaacgaaact tgtcaaaggt tcaggaagaa
    10681 acacaattgg cttcaaatca attggtccag caagctaaag atgcgatgca aagcgataca
    10741 atcaagcata tggtgcgtgt tcaggaaatt ctcgacggtc tgggtaggct ggacagcact
    10801 ctggttcggt atgaaattaa aggcgcacag gtcgaatggc aggcattggt gccgccagcc
    10861 tatattcaaa cagtgatgac aaaatatccg gtgcgggtcg atgatacggt aaaggaaaaa
    10921 gatgggcgta ttcgcgttat aggaaatcgc taaaaataca aaaagactat acctttgata
    10981 ggggggctgt gtactgtttt taaaaggaat ttaacctctt tattttatac taaatataac
    11041 tataatttta cgaatgaaca agatcgggga atagaagttg gattttagta agctacagca
    11101 gtcttttaag gaatttgatt ggcgctcgct taaaaaatat accagtccgc aagctgcgga
    11161 cgatcttaat gcatttctgg aaaaaatgcc gcaaaacgcc ggtcagacga tgttgattat
    11221 tgccggtctt atgtgggcta tgggcggggc gattggtttg ttcactacag ttcagctgca
    11281 aaaactcacg gaaacgcgtg cgcaattgca agaggcgcaa gcgctaaagc ccagtgtacc
    11341 acaggtcaga gatgttcctg taaatcctaa tgaagtggac gaatttgtta ccaaggtcaa
    11401 agatatttat acgggactga aaatcagtgc cagtggacca tctatttcaa taacagcaac
    11461 aactttaggt tcatttggac aatttcgtga ggctatcggg cacgttcaaa acgggggtgc
    11521 aggatggaac gtgaatattc agaacttttg cgtagggcgc gagtgtgaaa gatatccact
    11581 ttcggcatct ttgaagataa acaaggtttc cgtgaagtga ttgggatgat atacttaaag
    11641 ttacaaggtg gagaataaaa atatgttgaa gacacttaaa cgtttttata aaaacaaaaa
    11701 aaccttagga agagcggctg aacgtgggaa tgtgctgttc ctgatcctga tcgctgtggc
    11761 gctgtttgcc gccctttcat acgctgtgac gcaatcctcg cgctccggcg gtggtgcttc
    11821 tgatggagaa agtaatcttg tgaattcagc gcagatcacc cagtatccgg cctctgtgcg
    11881 cacggccatt gtccgtatga ttattggagg cacagatgtc ggtcagctca attttgatcc
    11941 tccatctgct tttagtattg cggtcaccgg ctgtgacgtg gcttctgttt cttcaacatg
    12001 tgtgttccac cctgacggtg gtggtgccac gcgtgtaacc gcgccgcccg aggttatggc
    12061 cagtggatcg caaggagatt ggattttcac cagtgatttt gctattgaaa acattggtcg
    12121 gaccacggcc tctaacaact cgaacgatgt cattgcgttt ttgcccggag ttgggcaatc
    12181 cttgtgtgag cgtctgaaca ccgagcttgg tgtgccgacc tcgacagatg ctgatggcga
    12241 tagtgtgccc gatgcaggta ttgtaatagg caacgttcca acggccgctg acgaaatgga
    12301 caacaacaac ctgggcattg gcccggctgc tgctggcgct ggtaaacgcc tgaccaacga
    12361 cctgtctggc cagccttttg gctgtgccga ctttgatgcc acgaccaatc aggcggatga
    12421 cagtgacctt gtatacttcc acgttcttgt cgaacgttaa ggcgggaaat gaatttgcag
    12481 ccttataaga tagtaacaaa gcgcgcttct gaagctggaa gcgcgcttgt ctatatcctg
    12541 attgcaatcg cgctgctcgc ggctctgacg atcagcttca tggagccgtc ttcccagcaa
    12601 acctcgtcac aaaacacatt taaaaccgta tccgctctgc aagggcaaat tgacattatt
    12661 cgctccgcca ttcaggaatg cgttttgata tatccgaacg gcgatccgac aattaacacc
    12721 gcgcccgccg gaagcgatcc aaacccctat gtgaaattcc cgatcaatcc ggattccacg
    12781 cactacacgt ctgcgacgcc tggccgctcc ggtgatcgct tggttcgtaa catgcgttgt
    12841 cccggtaatc ctacaacagc caatgtctac gatcatggtc tgatttttac cggctccagc
    12901 ggaaaattct tggggccggc cccggatctg tttgatgact ggcagattta taacggtgag
    12961 gatggcgtat tttactggac cgaaaccgac aaaactgacg cctttgttaa ggcggccttg
    13021 gaaaaactgg atgaaaaatt ctccgaatgc gaagccgatg tcattgatac ttccgatgca
    13081 cccgccggag ccaaagatct tgacagcaac gctctgctga cctgtccagc taatcatctg
    13141 tgcttccgtg tctggataat ccagaatgag gccacaaatg tctataatgg cgatgccgcc
    13201 ggcgatgagg gcggcgggtc ctgtcacggt tttcatgaga tcaatccttt ccggctctca
    13261 gccgtttgcc cggcgggcga agtatccccc cacgaggagg aaggacacgt tgggta
//
LOCUS       DAOSWR010000050        13216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_2974_length_13216_cov_6.057594, whole genome shotgun sequence.
ACCESSION   DAOSWR010000050 DAOSWR010000000
VERSION     DAOSWR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_13216_cov_6.057594"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1031
                     /locus_tag="PK513_05200"
     CDS             378..1031
                     /locus_tag="PK513_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO81877.1"
                     /translation="MAIKRDIQGRKPAEDRKREIVSTALKLADKVGPDRLSAAMIARN
                     VEISQPAVFKHFSSMNALWLAVAAHIETAMKKKWEAAFSAEKDEREQLRDLVLAQLRF
                     IQTVPAVPGILFSRELHAGNKGLRQAFLKIMSALHNRITNIIASGQESGVFSRDVPAV
                     DSAYLVLGFIQGLAVRWSVSGKNFPLVDEGERLFAIQMRFLMEEPSSGGKGDEHEQT"
     gene            1018..2175
                     /locus_tag="PK513_05205"
     CDS             1018..2175
                     /locus_tag="PK513_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO81878.1"
                     /translation="MNKRNRKAFLALISILAVIAATVFFVKNKPLSINITPVKEDVTV
                     EVYGLGTVEAKILSTVGFEVGAALVELNADQGDRVEKGAVLARLHSAEQEAKTALAAA
                     AVQSAESRLKKAQAAIPKLQSTLDFHKVSNKRSKELLPRGSIGEEEAEQDQLNEDVAA
                     AELEIARNDVLLMKAELAEAQAQYDFETTLLTHHVLKAPYDALVVERHKEQGTVIAPG
                     ETVFTLIDPATVWVLGYVDEARAGAIRAGQPARVRLRSLPHEAFEGKVARIDIESDRV
                     SEERRVYISCTRCPENFHLGEQAEIFVQTAILKKPLLVPETAVERFDGTSGYVWTVQE
                     KRLRRIPVTFGHKTLDGYLEVTGGIDAGTDIVAALARGLREGRRAIVRKER"
     gene            2177..3316
                     /locus_tag="PK513_05210"
     CDS             2177..3316
                     /locus_tag="PK513_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81879.1"
                     /translation="MNLAYHDIRHSMGRFILTCAGLSLLLGVVLSMIGIYRGLVDDAL
                     TLARTPAANIWVVEAGTRGPFAESSRIPGDTREAVARLHGVAAAGSVTYQSIETRHGA
                     GKLRLYVVGYEPGRPGGPDRITEGRAIARSHYEIIADRRTGLKTGERVLIGDNHFTVA
                     GLTDGQVSSGGDPVIYMSLLDSQELQFELAPPAARREVARGNAPDTADMDTVNAVIAR
                     LKPQASAEEIVNTVERWKHFAATTQQEQENILTRSVVDKARKQIGLFTAILLIVSAVI
                     ISLIIYTMTMDKIREIATLKLIGAPDKTIIGLIVQQALAMGVIGFGLGALLIVSIKDY
                     FPRRVVLQPEDGLLLAAAVLIVCILSSGLGVRLALKVDPATALGG"
     gene            3326..4060
                     /locus_tag="PK513_05215"
     CDS             3326..4060
                     /locus_tag="PK513_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO81880.1"
                     /translation="MKNNNHKKAESSPLVHLHGISKHFGEGSVRVDALKNIDIEVSAG
                     QVVALLGPSGSGKTTLLNVIGCILEPNEGYMVLDDDVVYDGHWLRSDLRALRLEKIGF
                     IFQFHNLLPFLSAQDNVGLVLELAGYDKTAARDRAEELLSYLEVDHRKDSFPAKLSGG
                     EAQRVAIARALANRPRIILADEPTAALDSGRAGIVMDLLRKVAAEQDAAILAVTHDEK
                     IYDRFDHIFHLRDGELEQETQQEEKT"
     gene            4057..4527
                     /locus_tag="PK513_05220"
     CDS             4057..4527
                     /locus_tag="PK513_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81881.1"
                     /translation="MRIMKLGLTGLAIMAFLTGGPMTAHAASPHDTHHGYHGESNMTM
                     KEVMQGLNAELKNLTDAIILEDYTAIEQSARGIAHHPPPSEAELKRIFSILGEDSEAF
                     KACDQAVHDLAAKLAGIAAGKNMKDILDTYHAMIVKTVECHASFKDNISGKKLD"
     gene            complement(4564..5145)
                     /locus_tag="PK513_05225"
     CDS             complement(4564..5145)
                     /locus_tag="PK513_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO81882.1"
                     /translation="MSIAVYIRVSTHSQKSDSQKAEISRWLKAHGHDLSEVMWYEDHE
                     TGTTMNRKEFNLLTEAVFAGAVKTVVVWKLDRLARSMKEGINVLSSLCESGVRVVSVT
                     QQIDLSGTVGQMVAGVLFGIAQIEHQHIKERQAAGIAVAKAKGIYTGRKKNTTKAKPE
                     RAKTLKEQGLTHAEIAQALNVTIRTVGNYLKEA"
     gene            complement(5254..8172)
                     /locus_tag="PK513_05230"
     CDS             complement(5254..8172)
                     /locus_tag="PK513_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOO81883.1"
                     /translation="MSKAAQDSYFWHLSDQEHQFIKKYRGLNRLKCATLLKYYQIEGR
                     FPEKFSDIPDIGRKILSENLKVPDDVDPDYDYMDRTGKRLRQSVREFLGIRNSLPEDW
                     ETAQSDLVEVCFQDFDAENALSQLLKQWFQDRNIERPTVLREERILNAVSVNLEERRN
                     QHIANLLSDTHQQSLDDLLKTQTGETSALLVLLKDDPGKPCLSSVFIELSKLESIDNL
                     CLPLDLFPADWQKLRKTYRQRATREPVRELRRHPDHIRYALLAAFCMERREEILDNLT
                     DLLIQIVHKIQVKAERRVTKEIAGGVREVSAKKALLFKIAQTAIENPDATIRDALFPI
                     IGENTFNDLVKEFEASSPFYNEQVELVARNSYKSHYRRMVPPILEVLKFRCNNQHHRP
                     AIDAITILKDLKFGQRVLRVDVLPISGIVPKSLQPLLIEDGKINRISYEICVLRALRN
                     ALRCREMWVEGARRYRNPDEDVPQDFEEKRTAYYEDLSLPIRSNDFITNLRSRMEKAL
                     RTLNSGLYKNNKVFIRLHGKNLIKVSPLEPQAESAFLKHLKSQVMMQWPMTSLLDVLK
                     EADCQIGFTNLLKSYGDREILSRDVLQKRLLLCLYALGTNTGLKRVLAGGADVTLDEL
                     RYVKERYVHKDGLKAAIAKIVNATLQVRQSQIWGESTTSCASDSKKLHAWDQNLMSEW
                     HVRYKGRGVMVYWHVEKNSLCVHSQLKRCSSSEVSSMIEGVLRHCTDIEIDKQYVDSH
                     GQSEVAFAFCELLGFSLMPRLKAIAQQKLYLPDNDMRSELHNLQPILKKHPINWEMIS
                     QQYDQMVKFATALQKGTASAESILNRFTRNNRQHPTYQALAELGRAVKTIFLCEYLDS
                     EETRREIHEALNVVENCNSTVDFIFFARGNEISSNQLEAQELSILSLHLLQVCLVYVN
                     TIMIQQILAEPKMMNKMREEDFRALTPLIYGHITPYGSFDLDMDQRLFPENDNLLMAA
                     "
     gene            complement(8413..8892)
                     /locus_tag="PK513_05235"
     CDS             complement(8413..8892)
                     /locus_tag="PK513_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOO81884.1"
                     /translation="MLTHLEKKVLPYTPEQMYALVAEVDKYSEFLPWCVASRINKHEG
                     DSVFYADLVVGYKMFRERFSSKVTLESPSEHAPGTIDIEYLRGPLRHLKNHWRFISEP
                     DGSCLIDFSVEFEFKNKALQGLSQAFFQEVIRRMVDAFEQRALAVYGSRRHCGQPPF"
     gene            complement(8911..9876)
                     /gene="lipA"
                     /locus_tag="PK513_05240"
     CDS             complement(8911..9876)
                     /gene="lipA"
                     /locus_tag="PK513_05240"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOO81885.1"
                     /translation="MTYNEKLLDKARRHPEKQKNPDRPAGRKPDWIRVPAPQGKIYGE
                     TKDIVRRAKLNTVCEEAGCPNVGVCWQHKHAAFMIMGEVCTRACAFCNVATGKPGALD
                     PLEPLRIGVAVKQMGLKHVVVTAVDRDDLPDGGAEHFVKTVQGIRMKSPETTVEIFPG
                     DFGGNQSDWDTVIGSRPDVFNHNIETVARLHPTIRPGARYFRSLRMLQRVKDVDPSIF
                     TKSGLMVGLGETMEEIAQMMDDMRAADIDFITIGQYLQPTPKHAPVMKWWSPEEFAEL
                     TKLAKRKGFLMISASPLTRSSHHAGDDFAKLKAAREAQLAAQAAE"
     gene            complement(9877..10179)
                     /gene="yecR"
                     /locus_tag="PK513_05245"
     CDS             complement(9877..10179)
                     /gene="yecR"
                     /locus_tag="PK513_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HOO81886.1"
                     /translation="MKNFLLLVVIFLSGCAVNKTMYATGGSKADGTVTLSYSYGGFEQ
                     PVVDYASALTTAKDKCKAWGYKNAEAFGGESLKCLAYNAYGCTQQQVDVMYQCLDK"
     gene            complement(10203..11606)
                     /gene="lpdA"
                     /locus_tag="PK513_05250"
     CDS             complement(10203..11606)
                     /gene="lpdA"
                     /locus_tag="PK513_05250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOO81887.1"
                     /translation="MSDKKFDVIVIGGGPGGYVAAIRAAQLKMKVALVEANHLGGICL
                     NWGCIPTKALLRSSEVFHLAQHADEYGLKIKGLEFDLQKIVERSRGVAKQLSGGIGHL
                     LKKNKVTVFDAYSKLEGGGKVALEKDGKKTDTLEGKHIIVATGARARVIPGFEPDGKF
                     VWTYKEALVPDTMPKKLLVIGSGAIGIEFASFFKTLGAEVTVVEMMDRIVPVEDAEIS
                     GMLEKALTKQGMKIIKNAKVSKLDKGKTEVTAHIEEQGGKVEKLTFDRVISAVGIVAN
                     TEGLGLEKTKVKLDRGHIVTDGYLKTDEPGVYAIGDVTGAPWLAHKASHEGIICVEKI
                     AGVKDVHPMVKSNIPGCTYCHPQVASVGLTEAAAKEQGYKVKVGRFPFMGNGKAIALG
                     EPEGLVKTVFDEKTGELLGAHMIGAEVTEMIQGYVIGKTAELTEAEFMHTVFPHPTLS
                     EMMHESVLDAYGKAIHF"
     gene            complement(11616..12209)
                     /locus_tag="PK513_05255"
     CDS             complement(11616..12209)
                     /locus_tag="PK513_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81888.1"
                     /translation="MSSQELVLSAQNIEGKYIKLLWSGLLFLAIGLACCIGSFYYFYM
                     IGEYDEDKQSVRQILPFLLCFIPVYGAQLIKKGLREKKLFVRSAVKELRFDKEGISGP
                     IIFLEGRVRDNLREMNKPSFNISWDDIDKFIVEPVRGSKTYGSPPYYKVTRKKTDGGL
                     YTSYFILRNYFRDREQDILGFVGGKISQDHIIINDEP"
     gene            complement(12206..12748)
                     /locus_tag="PK513_05260"
     CDS             complement(12206..12748)
                     /locus_tag="PK513_05260"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81889.1"
                     /translation="MIERKPIVGVMGSHDERWEEYARPLGELIARRGFHLMTGAGGGI
                     MIEVCKAFQSVKGRKGVALGIRPAVDYKGEELSKDEFPNSYIDLPIITPLSAKAQSDA
                     MPYSRNLVNVMSSKALVILPGSHGTRNEVSLGLMYEKPLMLFGPDEAFDQFPEEPLRS
                     DDISHVEQFFDDVFGGEGQV"
     gene            complement(12760..>13216)
                     /locus_tag="PK513_05265"
     CDS             complement(12760..>13216)
                     /locus_tag="PK513_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006280214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOO81890.1"
                     /translation="NVSWTDEAIHQYLSSDISVAVATPNGLITPIVRAAETKGLRAIS
                     DEVKELAGRAREGKLTPEEFQGGSFTISNLGMFGVKEFAAILNPPQSCILAVGAGEQM
                     PRVVNGEIKIQTVMSVTLSTDHRSVDGAVGAQWLQYFKQYIENPVSMLV"
BASE COUNT         3358 a         3550 c         3036 g         3272 t
ORIGIN      
        1 ctggacgcac tccatccacg gggcaagccc cttggttttt acgtgcctat aaaaaaacaa
       61 aacaatgcaa tatgcaacgt cttgatacgg tgtccgacca gaacatattt gctatgtgag
      121 tacgaaagct gaccctgaac agatagaaac acaacggaaa ctgtctctat ctttgcgctt
      181 aataggggta aagtgagcgt agctgtcaaa tggggccaaa tcacagttac tctcaaaaat
      241 ttcccacaat cctactctta aaacatggtt cgcaggttat gtgatcgcca cacaaatact
      301 ttttgacttt tcaaacgcct taatataagt tagttattag ttactaacca aacaatatta
      361 aggcttacat tttataaatg gctatcaaaa gagatataca ggggcgtaag ccagcggaag
      421 accgtaaacg tgaaatcgtt tcaacggcgt tgaaacttgc cgacaaagtc ggcccggata
      481 ggctttcggc ggcgatgatc gcccggaatg ttgagattag tcaaccggct gtttttaagc
      541 atttttcgag catgaacgct ctctggctgg ctgtggccgc tcatatcgaa acggcgatga
      601 aaaaaaaatg ggaagcggct tttagtgcgg aaaaagacga gcgcgagcaa ttacgtgatc
      661 tggtgctggc gcaactgcgg tttatccaga ccgttccggc tgtgccgggt attttattct
      721 cccgcgaatt gcacgccggc aataaggggt tgcgccaggc ctttttaaaa attatgagcg
      781 cacttcacaa tcgcattacg aatattatcg cttcgggtca ggaaagcggc gttttcagcc
      841 gtgacgttcc tgcggtcgac agtgcttatc ttgtcctggg gtttatacaa gggctggcgg
      901 tacgatggtc ggtaagtgga aaaaattttc ctctggtgga cgaaggtgaa cggctgtttg
      961 caatacaaat gcgctttttg atggaagagc cgtcatccgg aggcaaagga gatgagcatg
     1021 aacaaacgta atcgcaaagc ctttcttgcc ctgatttcta ttctggccgt catagcggca
     1081 acagtgtttt ttgtaaaaaa caaaccgctg agcattaata tcactccggt aaaggaagat
     1141 gtgaccgtgg aagtttacgg gctgggcacc gtggaagcca aaatcctctc gacggtcggt
     1201 ttcgaagtgg gcgcggccct tgtcgaactg aacgcggatc agggcgacag agtggaaaaa
     1261 ggcgctgtgc ttgcccggct ccatagcgcg gagcaagagg caaaaaccgc tttggccgcc
     1321 gcagccgtgc agtccgcaga gtccagattg aaaaaagcgc aggccgccat tcccaagctg
     1381 caatccaccc tggattttca caaggtcagc aacaaaagaa gcaaggagct gcttccccgc
     1441 ggaagcatcg gagaagaaga agccgaacag gatcagttga atgaggatgt cgccgcggcg
     1501 gaactggaaa ttgcccgcaa cgatgtcctg ctgatgaagg ccgagcttgc cgaagcgcag
     1561 gcgcaatatg attttgaaac cacgctactc acgcaccatg ttttgaaagc gccctatgat
     1621 gcgctggttg tcgaaaggca taaagaacag ggaaccgtta ttgcgcccgg cgagactgtt
     1681 tttacattaa tcgaccccgc aacggtctgg gtcctaggct atgtggacga agcccgcgcc
     1741 ggggctatcc gggccgggca gcctgcgcgg gtacgccttc gctctttgcc gcatgaggcg
     1801 tttgaaggca aggtcgcgcg catcgacatt gaaagcgacc gggtcagcga agagcgccgc
     1861 gtctatattt catgcacacg ctgccccgaa aattttcatc tgggcgaaca ggctgagatt
     1921 tttgtgcaga cagcgatctt aaaaaagcct cttttggttc cggaaacggc ggtcgaacgc
     1981 tttgatggca caagcgggta tgtctggacc gtgcaggaaa agcgcctgcg ccgcattccg
     2041 gtgacattcg ggcataaaac gctggatgga tatctggaag ttacaggcgg catcgacgcc
     2101 ggcacagata ttgttgcggc ccttgcacgc ggcttgcgtg aaggacgcag agccatcgtc
     2161 aggaaagagc ggtaagatga atctcgccta tcacgacata aggcacagca tgggtcgttt
     2221 tatactgacc tgcgcggggc tgagcctcct gctgggggtt gttttgtcca tgatcggcat
     2281 ttacagaggt ttggttgacg atgcgctgac tctggcgcgg acgccggcgg cgaatatctg
     2341 ggtggttgaa gccggtacgc gggggccgtt cgcggaatcc tcccgtattc ccggcgatac
     2401 gcgtgaagcc gtggcgcgtt tgcacggagt cgcggcggcg ggttccgtaa cataccagtc
     2461 aatcgaaacg cgccacgggg cgggtaaatt acggctttat gtcgttggtt acgaacccgg
     2521 aagaccgggc gggcctgaca ggattacaga aggcagggct atcgcccgca gccattatga
     2581 gataatcgcc gaccgccgga ccgggctgaa aacgggcgag cgtgttttaa ttggcgataa
     2641 tcattttacc gtcgccgggc ttacggacgg ccaggtctcc tccggcggcg atccggtcat
     2701 atatatgagc ctgctggatt cgcaggaact acaattcgag ctggcgccgc ccgccgcccg
     2761 ccgcgaagtt gcgcgcggca acgcgccgga cacagccgac atggatacgg tcaatgccgt
     2821 catcgcccgg ttaaaaccgc aggccagtgc ggaagaaatc gtcaatacgg tagagcggtg
     2881 gaagcatttt gccgccacca cccagcagga acaggaaaac atcctgaccc gctcggtcgt
     2941 ggacaaggcc cgcaagcaga tcggcctgtt taccgccatc ctgctgatcg tttcggcagt
     3001 gattatctcc ctcatcattt acaccatgac catggacaaa atccgcgaga ttgccacgct
     3061 caagctgatc ggcgcgccgg acaaaacgat catcggcctg atcgtgcagc aggcgctggc
     3121 catgggcgtt atcggttttg ggctgggggc gctgctcatt gtcagcatca aggattactt
     3181 cccccggcgc gtagttttgc agccggagga cggcctgctg ctggccgctg ccgttcttat
     3241 tgtttgtatc ctctccagcg gtcttggcgt gcggctggcg ctcaaggtcg atcccgcgac
     3301 ggcgttggga gggtaaatag cggccatgaa gaacaacaat cataagaaag cagagtcatc
     3361 ccccctcgtt catctgcacg ggatcagcaa acatttcggt gaaggcagcg tccgcgtcga
     3421 cgccctgaaa aatatagaca tcgaagtttc ggctgggcag gtcgtcgccc tgctggggcc
     3481 gagcggatcg ggaaaaacaa ccttgctcaa tgtaattggc tgtattctgg aaccgaatga
     3541 aggatacatg gttctggatg acgacgttgt gtatgacgga cactggctgc gttcggattt
     3601 acgggctttg cggctcgaaa aaatcggctt tatctttcag tttcataatt tgctgccgtt
     3661 tttaagcgcg caggacaatg tcggccttgt tctcgaactg gcaggctatg acaaaacagc
     3721 ggcaagagat cgcgcggaag aactgctttc ctatctggag gtcgatcacc gcaaggattc
     3781 ctttccggcc aaactctccg gcggcgaagc gcagcgtgta gccatcgccc gcgctctggc
     3841 caacaggccc cgtattatcc ttgcggacga acccacggcg gcgcttgatt cagggcgggc
     3901 gggcatcgtt atggatttgc tgcgaaaggt cgcagccgag caggatgcag ccattctcgc
     3961 cgtgacgcat gatgaaaaaa tatatgaccg tttcgaccat atttttcatt tacgcgacgg
     4021 ggaactcgaa caggaaacac agcaggagga aaaaacatga gaattatgaa actgggactg
     4081 acagggctgg ccattatggc tttcctaacg ggcggtccaa tgacggcgca tgccgccagc
     4141 ccccatgata cgcaccacgg ctatcatggc gaaagcaata tgaccatgaa ggaggttatg
     4201 caggggctga atgccgaatt gaaaaatctg acagatgcaa taattctgga agattatacg
     4261 gccattgaac aaagcgcgcg gggaattgcg caccatccgc cgccaagcga agcggaacta
     4321 aaacgtattt tttccatcct tggcgaagat tcggaagcgt ttaaagcctg cgatcaggct
     4381 gtgcacgatc ttgcggcaaa actggccggg atagcggcag gaaaaaatat gaaggatata
     4441 ctggacacct atcacgccat gatcgtcaaa accgtggaat gccacgctag tttcaaagac
     4501 aatatttcag ggaaaaaatt ggattagaac ctaaaattat cgtttgcagc atatagatcc
     4561 cagctatgct tctttcaaat aattcccaac cgttcggatg gttacgttga gtgcttgcgc
     4621 aatttcagca tgggtcaatc cctgttcttt taaagtttta gcccgttcgg gcttggcttt
     4681 agttgtgttt ttcttgcggc cggtgtaaat accttttgct ttagcaactg caattcctgc
     4741 ggcttggcgt tctttgatat gctgatgctc gatctgggcg atgccgaaaa ggactcctgc
     4801 caccatctgg ccaactgtgc cggaaagatc aatctgctgt gtcacggata caacgcgcac
     4861 accggattca cagagtgatg aaagcacgtt gatcccttct ttcatagatc gcgccaatcg
     4921 atccagcttc cagaccacaa ccgttttgac cgcgccggca aaaacggctt cagtcagtaa
     4981 attaaattcc ttgcggttca tcgtggtacc tgtttcatga tcttcgtacc acatcacctc
     5041 gctcaaatca tgaccatgcg ctttcagcca acgtgatatt tctgcttttt ggctatctga
     5101 tttttgtgag tgggtggaaa ctcggatata aacggcaatg ctcatagcaa attagggtac
     5161 accctatttt gctctatgtc acgcaggttt aaaactgccg gaatccaacg gttaaaatca
     5221 gcgatttacg gatagaccct atttttcctg tttttaagct gccatgagca aattatcatt
     5281 ttctggaaac aatctctgat ccatgtccag atcgaatgag ccatagggtg taatatgtcc
     5341 ataaatcaga ggggtgagtg cacgaaaatc ttcttcacgc attttgttca tcattttcgg
     5401 ttctgccaat atttgctgaa tcatgatggt gttgacgtaa accaaacaaa cttgcaaaag
     5461 atgcagggac aagattgaaa gctcctgcgc ctcaagctgg ttcgatgaaa tttcattgcc
     5521 tctggcaaag aaaataaaat ccacggtact gttgcaattc tcgactacat tgagggcttc
     5581 gtgaatttca cgccgtgttt cttctgaatc cagatactcg cataagaaga tggttttcac
     5641 ggcgcgccct aattccgcca aagcttggta agtcggatgc tggcggttat tgcgggtaaa
     5701 acggttcaga atagattctg cgctcgccgt tcctttttgt aaggctgtgg cgaatttcac
     5761 catctggtcg tattgctgtg aaatcatctc ccagtttatc ggatgttttt tgagaattgg
     5821 ctgcaaatta tgaagctcag agcgcatatc attatcaggc agatagagct tttgctgggc
     5881 aatggctttt agacgaggca ttagtgaaaa tcccaaaagc tcacaaaacg caaaggccac
     5941 ttcgctttgc ccgtggctat ccacgtattg tttatcaatc tcaatgtccg tgcagtgccg
     6001 cagaacacct tcgatcatgc tgcttacttc cgatgaagaa cagcgtttga gctgtgaatg
     6061 cacacagaga gaattttttt caacatgcca gtacaccatc acaccccgcc ctttataccg
     6121 tacatgccat tcgctcatca ggttttgatc ccaggcatga agctttttgg aatcagacgc
     6181 gcatgatgtt gtgctttcac cccagatttg tgattgtctg acttgcaatg tagcattaac
     6241 aatcttggca atggcagcct tcaagccatc tttatgcaca tagcgttcct taacgtatcg
     6301 taattcatca agtgtcacat ccgcgcctcc ggccaatacg cgcttcaacc ctgtatttgt
     6361 cccaagggca taaaggcaga gtaaaagacg cttttgcaaa acatcgcgtg ataaaatctc
     6421 acgatcacca tagcttttta gaagattggt aaatccgatc tgacaatcag cctctttgag
     6481 tacgtccagt aaacttgtca ttggccactg catcataact tgagatttca gatgtttaag
     6541 aaatgctgac tctgcctgtg gttccagagg ggagacttta atcaggtttt tgccatgcag
     6601 cctgataaac actttgttat ttttgtacag gccgctgttt aatgttcgca aagccttttc
     6661 catgcgcgac ctgagattgg tgataaaatc atttgagcgt atcggcaggc tcaaatcttc
     6721 ataataagcc gttctttttt cttcaaagtc ctgcggcacg tcttcatccg gattacgata
     6781 gcgccgcgcc ccttccaccc acatctcacg gcaacgcaga gcattgcgta atgcgcgaag
     6841 aacacagatt tcatagctga ttcggttgat ctttccgtcc tcaatcaaaa gaggctgcaa
     6901 agattttgga acaataccgg agataggcag cacatcaaca cgcaataccc gctgcccgaa
     6961 ttttaggtct ttcaatatcg taatcgcatc aatcgccggc ctgtgatgct gattattaca
     7021 acgaaatttt aaaacctcta gaataggcgg caccatccgc cggtaatggc ttttgtaaga
     7081 attgcgtgcc accagctcta cttgctcatt gtaaaaggga ctgctggcct cgaattcttt
     7141 gaccagatca ttgaatgtgt tttcgccgat aatcggaaac aaagcgtcac gaatggttgc
     7201 atcaggattt tcgatggcag tttgcgctat tttaaagagc agtgctttct tggccgacac
     7261 ctcacgcaca ccacctgcga tttctttggt cactctgcgc tcagccttaa cctgaatttt
     7321 atggacgatc tggatcagta aatctgtgag attatccagg atctcttcgc gccgttccat
     7381 gcaaaacgca gccagaagag catagcggat atgatccgga tgacggcgaa gttcgcgcac
     7441 cggttctcgc gtggcgcgct gacggtatgt tttacgcagt ttttgccaat cggcagggaa
     7501 caaatccagt ggcaagcaca aattatcaat actttccagc ttggataact caatgaaaac
     7561 actgctcagg cacggtttgc ccggatcatc tttcaacaaa accaatagag ctgatgtttc
     7621 gccggtctgt gttttgagta aatcatccaa actttgctga tgtgtatcag agagtagatt
     7681 ggcaatatgt tgattgcggc gctcttcaag gtttacagac actgcattga gaatgcgctc
     7741 ttcgcgcaaa actgtcggac gctctatatt tcgatcttga aaccactgct tcaaaagctg
     7801 tgacaaggcg ttttctgcat caaaatcctg aaagcaaact tcaacaaggt cggattgtgc
     7861 tgtttcccaa tcttccggta atgaattccg tattcctaaa aactcgcgga cactctgccg
     7921 taagcgtttc cctgtgcgat ccatgtaatc atagtcagga tcaacatcat caggaacttt
     7981 cagattttca gataaaatct tacgcccaat atcaggaata tcgctgaatt tttcagggaa
     8041 acgaccctcg atctggtagt atttgagcag agtcgcacat tttaggcggt ttaaaccacg
     8101 atatttcttt atgaattgat gctcttggtc agacaaatgc caaaaatatg aatcttgtgc
     8161 cgccttggac atgctggaaa tctcccgttt ttgtaaactg aaagattatc catagcactt
     8221 ttggccccat ttgacagcta cgctcacttt acccctaata ggctcaaaag gggaagctca
     8281 ggaattaaac agtcctgtta agaatacagg ccaaagccct tatcatcctc gcatagatct
     8341 cttcggctta cgttccagac cctttatcta tgccgcaaca cgaagacaac aaattcactt
     8401 ccaatatgga ctctaaaatg ggggttgacc acaatgacga cgtgaaccgt acacagccaa
     8461 tgcacgctgt tcaaacgcat ccaccatccg gcgaataact tcttgaaaaa atgcttgaga
     8521 taaaccctgt agtgctttgt ttttaaattc aaattcaaca ctaaaatcaa taagacatga
     8581 cccatctggc tcgctaatga accgccaatg gttcttcaga tgcctcaacg gtccgcgcaa
     8641 atattcaata tcaattgtcc ctggcgcatg ttctgaaggg ctttccaacg tcacctttga
     8701 agaaaaccgt tcacgaaaca ttttatagcc caccactaaa tcagcgtaaa agacgctatc
     8761 accttcatgt ttattgatcc ggctcgccac gcaccacggc aaaaactctg aatatttgtc
     8821 cacctccgcc accagcgcat acatctgctc tggcgtataa ggaagaactt ttttttcaag
     8881 atgggtgagc atcgagtatc cagactgctc ctattctgcc gcctgagcgg ctaattgcgc
     8941 ctcacgcgca gccttcaact tcgcaaaatc atctccggca tgatgcgagg accgggtcag
     9001 cggcgatgcc gaaatcatca aaaacccttt acgcttggcg agcttggtca gctctgcaaa
     9061 ttcttccgga ctccaccatt tcatcaccgg cgcatgtttc ggcgtcggct ggagatattg
     9121 cccgatcgta ataaaatcaa tgtcggccgc gcgcatatca tccatcatct gagctatttc
     9181 ctccatcgtc tcgcccagcc ccaccatcag gccggatttg gtaaagatcg acggatcgac
     9241 atctttaacc cgctgcaaca tccgtaaaga ccggaaatag cgcgcccccg gacgaatagt
     9301 gggatgcagc cgcgccacag tttcaatatt gtgattaaac acatccggcc gcgatccgat
     9361 aaccgtatcc cagtcgctct ggttgccgcc aaaatcaccg ggaaagatct ccacggtcgt
     9421 ttccgggctt ttcatgcgaa taccctgcac ggttttgacg aaatgctccg ctccgccatc
     9481 aggcaaatca tcccgatcca ccgccgtcac cacaacatgt ttgaggccca tttgcttcac
     9541 cgccaccccg atacgcaacg gctcaagagg atcaagcgcg ccgggcttac cggtcgccac
     9601 attgcaaaac gcgcaggccc gcgtgcagac ctcgcccata atcatgaacg cggcgtgttt
     9661 atgctgccag cacactccga cattcggaca gcccgcctcc tcacataccg tgttcagctt
     9721 agcccttcgc acaatatctt tggtttcgcc gtaaatcttc ccctgtggcg cgggtacacg
     9781 gatccagtcc ggcttacgcc ctgccggccg atccgggttt ttctgctttt ccggatgccg
     9841 ccgggcttta tcgagaagtt tttcgttata ggtcatttat ttgtctaaac attgatacat
     9901 cacatcaact tgctgttgag tacatccata tgcgttataa gcaagacatt ttaaactttc
     9961 accaccaaag gcttctgcat ttttataccc ccatgcttta cacttatctt ttgctgtagt
    10021 taaagcagaa gcataatcaa ccacaggctg ctcaaaacca ccataagaat aagacagtgt
    10081 tactgttcca tcagctttac tgcctccggt agcatacatg gttttattta cggcacaccc
    10141 actcaaaaaa attacaacca acagtaaaaa gtttttcatc tgatatctcc aactttaaca
    10201 tgctaaaaat gaatagcctt cccatacgca tccagcacac tctcatgcat catttcgctg
    10261 agcgtcggat gcgggaacac ggtatgcatg aactcggcct cagtcagctc ggcggttttg
    10321 ccgatgacat agccctggat catttcggtc acttccgcgc caatcatatg cgctccaagc
    10381 agctcgccag ttttctcatc aaacacggtt ttcacaaggc cctcaggctc acccaaggca
    10441 attgccttcc cgttgcccat aaacgggaag cggccaacct tgaccttata gccctgctcc
    10501 ttcgccgctg cttcggtgag gcccaccgat gccacctgcg gatgacaata ggtacagccc
    10561 ggaatattcg acttcaccat cgggtgtacg tcttttaccc cggcgatctt ttcgacgcag
    10621 atgatcccct catgcgaggc tttatgcgcc agccacggcg cacccgtaac atcgccaatc
    10681 gcgtaaactc ccggctcatc tgtcttcaga tacccgtcag tgacgatatg tccacgatcc
    10741 agcttcacct tggttttttc cagccccaat ccttccgtat tggccacaat gcccaccgcc
    10801 gaaatcactc ggtcaaaggt tagtttttca accttgccgc cctgctcttc tatatgtgcg
    10861 gtcacttccg tcttgccttt atcaagcttg gagactttcg cgttcttaat gatcttcatc
    10921 ccctgcttgg tcaacgcctt ttccagcatc ccggaaatct ccgcatcttc tacaggcacg
    10981 atccggtcca tcatctcgac aacggtgacc tccgccccca gcgtcttaaa gaacgaagca
    11041 aattcaatcc caatcgcacc agagccaatc accagcaact ttttcggcat cgtatcaggc
    11101 accaacgctt ccttatacgt ccacacaaac ttgccatctg gttcaaaacc cggaatgacc
    11161 cgcgcccgcg cccctgttgc gacgataatg tgtttgccct caagcgtgtc agttttctta
    11221 ccatcttttt cgagcgccac ttttccaccg ccctcaagtt tgctgtacgc gtcaaacacc
    11281 gtgactttgt tttttttcag caaatgccca atcccgccgg agagctgctt ggccaccccg
    11341 cgcgaacgtt caactatttt ttgaagatca aattccaagc ctttgatttt aaggccatat
    11401 tcatcagcat gttgtgctag atgaaacacc tcgctggagc gcagcagcgc cttggtcgga
    11461 atgcaccccc aattcagaca aattccgccc aaatggttgg cctcaaccag tgccactttc
    11521 atcttgagct gcgccgcccg gatcgccgcg acataaccgc caggaccgcc accaatcaca
    11581 atcacatcga atttcttgtc actcatatcc atcctctacg gttcgtcatt aataattata
    11641 tggtcctgag aaatcttgcc ccctacaaaa ccaagaatgt cctgctctcg atccctgaag
    11701 taattacgca aaataaaata ggatgtgtaa agcccgccat ccgtcttttt tcgtgtcacc
    11761 ttgtaataag gcggcgaacc atatgtttta ctgccccgaa ccggctcaac gataaactta
    11821 tcaatatcgt cccaagatat gttaaaagaa ggcttgttca tttcacgtaa attgtccctc
    11881 acccgtccct caagaaaaat aataggcccg gaaataccct ctttatcaaa acgcaattcc
    11941 ttaacggcgc tgcggacaaa aagcttcttt tcccgcaaac ccttcttaat aagctgcgct
    12001 ccataaacag gaataaaaca caaaagaaaa gggagaatct gcctcacact ttgcttgtct
    12061 tcatcatatt caccgatcat atagaaataa taaaaactgc cgatacaaca ggccagaccg
    12121 atcgccaaaa acaacagtcc gctccaaaga agcttaatat attttccttc aatattttga
    12181 gcgctgagaa caagttcctg agaactcata cctgcccctc ccctccaaac acatcatcaa
    12241 aaaactgctc aacatgagaa atatcatcag agcgcaaagg ctcttccgga aactggtcaa
    12301 acgcctcatc cggtccaaac agcatcagcg gtttctcata catcaaaccc aaggacactt
    12361 cattacgcgt cccatgcgaa ccgggcagta taacaagcgc cttagatgac atcacattca
    12421 ccagattgcg gctgtaaggc atcgcatcgc tttgcgcctt ggcgctcagt ggcgtaataa
    12481 tcggcaaatc gatatagcta ttaggaaatt catctttcga aagctcctcg cctttataat
    12541 ccaccgccgg acgaatgccc aaagcaaccc ccttgcgccc tttaacgctt tgaaaagcct
    12601 tgcaaacttc aatcataatg ccgccgccag ccccggtcat aagatgaaat ccgcgccgcg
    12661 caatcaactc acccaatgga cgcgcatact cctcccaccg ctcgtcgtgc gagcccataa
    12721 caccaacaat cggcttacgt tcaatcatag cctctcccct taaaccagca tactcaccgg
    12781 attttcaata tattgtttaa agtattgcag ccactgcgcc ccgacagccc catccacaga
    12841 gcgatgatcg gtcgaaagcg tcacactcat caccgtctgg attttaatct ctccattcac
    12901 cacacgcggc atttgctcgc ccgcgcccac cgccagaatg cagctctgcg gcgggttaag
    12961 gatcgcggca aattctttca caccgaacat cccgagattg gaaattgtaa agcttccgcc
    13021 ctgaaattcc tcaggggtaa gcttgccttc acgcgcccgg ccggccagct ccttaacctc
    13081 atcggaaatc gcgcgcaaac cctttgtttc cgccgcccgc acaatcggcg tgatcaaacc
    13141 gttcggcgtc gccaccgcca ccgagatatc cgaagataga tattggtgaa tagcctcatc
    13201 ggtccagctt acattc
//
LOCUS       DAOSWR010000051        12989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3053_length_12989_cov_5.779109, whole genome shotgun sequence.
ACCESSION   DAOSWR010000051 DAOSWR010000000
VERSION     DAOSWR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_12989_cov_5.779109"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PK513_05270"
     CDS             <1..577
                     /locus_tag="PK513_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOO81891.1"
                     /translation="NPEGDDGIAGPKTKKAVEIAQKALGMEVDGKITPEFTARLDQKI
                     AAVQAQKADVKVVQAPSTTTTERFTVAASLADKQEPEAPKPKEQKSVQEPKLSAIDEH
                     IRIMTGGMDPDKQNRYDPNAPSLWVAFKNAAGSLFGSDDEKPEVAGEKREPAPVDELS
                     TWAATGEPVEPPQGQQNLPPAGTGGGPMRPV"
     gene            632..1771
                     /locus_tag="PK513_05275"
     CDS             632..1771
                     /locus_tag="PK513_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81892.1"
                     /translation="MGTRQPAGMGFVNNGARGTKDFRFGEDVLARSEVAKFLNGKPLT
                     YETAEQFKVFVSGHGAFNSADQEMIAAANPYLQPEVLDKFIYKEPPAHPPQKTAEPLI
                     QNLEKITREDGSLPLYWAQKIAKDAEQKVLNGENFSVEDRKMLETLLEHKIHQIPAYK
                     KPLEEALRLVNQGLERQANPKAKTGPSEKPTASQERQQETTQDFGAKTFPAKPPPVIK
                     AEPLPPAGGQTKPQTQPVPPTEGNQTGILARISKIDGPRDYLRDLDSNQVKEMQRVLG
                     VNDDGKWGQKTMAAAAQYIAKNNLPQNISLEDLQKHMQGVKPVSEAQQGYDEDRATHY
                     AQEAAKIEKGATKEEFKEAHGNPVPDAAPGEPKATPSTTPVVRLA"
     gene            complement(1768..2205)
                     /locus_tag="PK513_05280"
     CDS             complement(1768..2205)
                     /locus_tag="PK513_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO81893.1"
                     /translation="MAVKIRAIENRDFPDWLPLWNGNNLGMRDEAVTSETWSRLIDPN
                     SKVCGLVAEKDGALVGLVHYILHPTTGSLHDVCYMQDVFVSPDARREGIAKKLITYIS
                     QIGKREKWARIYWLAESNNAAAQSLYRALGQKLDFTLHVMKLD"
     gene            complement(2220..3320)
                     /gene="pheS"
                     /locus_tag="PK513_05285"
     CDS             complement(2220..3320)
                     /gene="pheS"
                     /locus_tag="PK513_05285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO81894.1"
                     /translation="MTDINALKTEITSEIEAARTPAALDDVRVTVLGKKGRITALMKT
                     LGGMDPEERKTVGAALNVLKDEIAELIKRQEKALKKQALDERLATETLDVTLSTRPER
                     SGKIHPISQTMEEMVGIFGAMGFSVAEGPDIEDDWHNFTALNFPPGHPAREMQDTFFL
                     PEDPSEEGELAKKLLRTHTSAVQIRHMKDNEPPYKIVCFGRTYRSDYDMTHSPMFHQM
                     EGLYIDKNVTMAHLKGCLTDFVRSYFKMDDLPVRFRPSFFPFVEPGAEVDIGYIKKPD
                     GGIKLGKAPDGQEQKWMEVLGAGMVHPNVLKNCSVDADVYQGFAFGMGIERLTMLKYG
                     IPDLRTFFECDTRWLAHYGFDPLDRPNNSTRS"
     gene            3427..3822
                     /locus_tag="PK513_05290"
     CDS             3427..3822
                     /locus_tag="PK513_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81895.1"
                     /translation="MSKTKVAANHYLAEVSQSVDGGGRLGRDFWVAVRDFNRYEPSDI
                     TLSQYSQQYHTPSNEQVRRKQKRAERLGELYPLAGEAWAQEAYRNVHGFFKRLEEERF
                     TEAQAQDLETDLREGVQSIVQDLLIPHLK"
     gene            complement(3814..4479)
                     /locus_tag="PK513_05295"
     CDS             complement(3814..4479)
                     /locus_tag="PK513_05295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO81896.1"
                     /translation="MILNNKLFVFDWNGTLLADTVPSWKAGNVCLEFYGAEAISLKTH
                     RETFCFPIIPFYEKHGVSEETVLERRDEGNAVFQDTYEQLAAGARTRHGARDLLEYLS
                     EQGAECIILSNYITERIERHLKRLNIEHYFSYVSAHDCNGTTILQSTTKVLRLRDYMS
                     ESGHAPENTIIIGDTMEEPEIARALGITSIGITDGYISRARLRQAAPDHIVHRLDEIV
                     KLF"
     gene            complement(4581..5330)
                     /locus_tag="PK513_05300"
     CDS             complement(4581..5330)
                     /locus_tag="PK513_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO81897.1"
                     /translation="MGEYSKEHIWIIGASSGIGRALAIELAARGGALILSARNEDALN
                     SLNDALGGGHQVIALDAGDHKALSAAAKAIPKLDRVIFMAALYAPHGKETKDIDFIHD
                     MIRVNLGGAYNTTNATLPIFEKQGHGQIALCGSVAGYRGLPYGQPYCATKAAMINYAE
                     SLKIELEPHNIDVKIINPGFVRTPLTDKNDFPMPMMIEADAAAKVIAGDLRSKRFEIH
                     FPKRFTWMMKILYFLPAPLYFFVARKIVSKL"
     gene            complement(5353..7974)
                     /locus_tag="PK513_05305"
     CDS             complement(5353..7974)
                     /locus_tag="PK513_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOO81898.1"
                     /translation="MKYIWYAFLGLFSLGFLGLLVCVAGVLYILNYYGSDLPDYKQLK
                     TYQPPIVTRLYAGDGRLMAEFAQEKRIFMPIEKIPDLVKNAFIAAEDKNFYTHQGVDP
                     TAIVRAAYVYGQHLMGKDVNVIGGSTITQQVVKNFLLTNERKFERKIREAILASRIER
                     AMTKDQILELYLNEIFLGYRAYGVAAAGLQYFNKSLDELSVAEAAYLAGLPKAPNNYH
                     PTRHHDRAVKQRNEVIRRMENNGFITEGQAELAKLAPLEVITRDEDRRVNAPYFAEEV
                     RRQLAGRYGEDSLYQGGLAVRTTIDPKLQEIAERALREGLMAYDRRHGYRGPLKTVTN
                     VAEWKSKLEEFKAPQGIIDGWKIAIVLDVGSARAEIGFEDGTKGTLPLDGVKWARKSL
                     NEGYALGPEITAVDEVLSKGDIIAVSPIDGSKNNYVLEQIPLVQGAIIAMDPHTGRIL
                     AMQGGWNYHYGSSEFNRATQAQRQPGSAFKPFVYLAALENDFTPATLVLDAPFVIEDR
                     PGNFWSPTNYSNEYYGPTPIRVGVEKSRNLMTVRLADYLGMDTIVDYAKRFGIDEDMP
                     PLLANSLGAGETTLLKLTSAYGELVNGGKKIEPSFIDRIQDRRGKTIFRHDDRQCHNC
                     GDLIRWDLQEAPELPDPREQLVDERHAYQIVSIMEGVVQRGTGVRLKELERPLAGKTG
                     TTNESKDTWFMGFSPDLVTGVFVGFDEPRSLGKRETGSSVALPVFKSFMEEALADEPI
                     LPFRIPNGIRNVQINAETGARAQIGDKKVIWEAFTVGTEPTHDMFILDGTGINLFTGN
                     AAYYDYDAQSDAYSPGNPGGYDLEPFPQDNGQDGGLYTPEGTAAGQNPDQLPSRPPYN
                     PSPTTPSETNVGTGGLY"
     gene            complement(8130..8903)
                     /locus_tag="PK513_05310"
     CDS             complement(8130..8903)
                     /locus_tag="PK513_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO81899.1"
                     /translation="MSDTPPKIGLKNVCKAFGSNRVLNGVDLVVPEGKSMVIIGGSGT
                     GKSVMLKCILGLMTPDSGSIEIDGQETVGLSPKDRDTLMRKFGMLFQGAALFDSLKIW
                     ENVAFGLIQGRGMDRVQAREIAVQKIEAVGLRPDVSDLFPAELSGGMQKRVGLARAVA
                     ASPEIIFFDEPTTGLDPIMADVINQLIVEQVDDIGATALSITHDMGSVRTIADYVAMI
                     HEGKIVWTGPVEDLDDSGNAYVDQFIHGKADGPITRSVA"
     gene            complement(8900..9718)
                     /locus_tag="PK513_05315"
     CDS             complement(8900..9718)
                     /locus_tag="PK513_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO81900.1"
                     /translation="MEPQSSIQSSGLFDPLLNVFQAVGAFILNFFQRAGALSIFIGRA
                     IYHSFTPPFFPAQLWRQLVDIGYYSLPVVGMTALFTGMVLALQSYTGFTRFNAEGAIA
                     GVVVLSVTRELAPVLAGLMVAGRVGAAIAAEIGTMRVTEQIDALCTLSVNPFKYLIAP
                     RLIAATITLPILVLIGDIIGVYGGYVVSIYNLGFSPGTYLTQTWDIIETMDVVSGLVK
                     AGAFGFIVAVMGCYNGFNSGRGAQGVGAATTNAVVSSSILILIFNYLLTQVFFS"
     gene            9809..10531
                     /locus_tag="PK513_05320"
     CDS             9809..10531
                     /locus_tag="PK513_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO81901.1"
                     /translation="MKTLHNINPADLVDMSAQESAHPYRVEVAYACDDNLLFGQQIYR
                     SDAHLWLHQDLADIVSKAAADLNDQGYCLILYDGLRTVDAQAAMLETRAVKENPHWLE
                     EPRLLSPPGAGAHPRGMAIDCSLETLEGRLLDMGTPFDYLSTDPAPESNPAHRAYTDL
                     TEEARTNRKILDTAMAKAAQESGTPLWPLPQEWWDFRLPLEVSETYAPLSDADLPPEI
                     RMVRYSQLSSFQRNTKLVKPRT"
     gene            complement(10648..11706)
                     /gene="alr"
                     /locus_tag="PK513_05325"
     CDS             complement(10648..11706)
                     /gene="alr"
                     /locus_tag="PK513_05325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO81902.1"
                     /translation="MRGRLSIDLGALARNYRTIQNMVGENCTAAAVVKADGYGLGVAA
                     VAKALKGAGAQDFFVANLDEAAALRGILGEGPVIYTLNGFDARGAHVYQSEQIIPVLN
                     SLEDVQDFGAHGQDLPAPALHIDTGMNRLGIRMDRLDALQGHDFSLVMSHFACADEKD
                     HSMNAAQFEVFQAAAKHFPRTKKSLANSSGAFRSNAYHFDMVRPGMCLYGLNPKPETE
                     NPMEAVVSLSVPILQIRDTAPGETCGYNATYRFKNKTRLAVVALGYADGFLRSLSNGG
                     VLYWQGIPCPVRGRVSMDLTIVDLSAVAERALPTPGGMMEVIGMHQSADDLARDAGTI
                     GYEILTSLGARHLRRYID"
     gene            complement(11710..>12989)
                     /locus_tag="PK513_05330"
     CDS             complement(11710..>12989)
                     /locus_tag="PK513_05330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOO81903.1"
                     /translation="TLIERGQNASPVTLKNYFENDEDLSDVGGASYLADLAANVVSVI
                     NTADYARTIYELYLRRQLISLGEDVVNEAYEHSLDSDAMSTVERMESRLFELAETGDV
                     KGGFITLRDSVLTANEHAEKAFQSDGHVTGITSGLVDVDKQLGGFQNSDLLILAGRPS
                     MGKTALAVNWAFNAAKAYAESGGKQGGRVAFFSLEMAAEQLAGRILSEQSGISGDAIR
                     KGNIKQEDFRAFVEASQKMSQVPLYIDDTPALSIGAVRTRSRRLKRQHGLDMIVVDYL
                     QLLRGTGSRQSESNRVLEISEITRGLKAIAKELEIPVIALSQLSRAVEQREDKRPQLS
                     DLRESGSIEQDADVVMFVYREEYYLSRAEPEPGTEKHMKWQESMERAHNVGECIVAKQ
                     RHGPIGIVKMFFDPNLTKFSDLDPNHIGASFGE"
BASE COUNT         3399 a         3540 c         3077 g         2973 t
ORIGIN      
        1 aaaccctgaa ggtgatgatg ggatagccgg tccaaaaacg aagaaagcgg tagaaatagc
       61 acaaaaagcc ttgggaatgg aagtagatgg gaaaatcacg cccgagttta cagcacgtct
      121 ggatcagaaa atcgcggcgg tccaagctca aaaagctgat gttaaagttg ttcaggctcc
      181 ctcaacaacc acaacagaac gctttaccgt agcagcaagc ctcgccgaca aacaagaacc
      241 ggaagcgccg aagccaaaag agcagaaatc tgttcaagag ccaaagcttt ccgccattga
      301 tgaacatata cgcatcatga ccggcggtat ggatccggat aaacaaaatc gctacgaccc
      361 aaacgcgccg tcgctctggg tagcctttaa aaatgcagca ggaagcctct ttggttcaga
      421 cgatgaaaag cctgaagtcg ccggggagaa acgagaacct gcgccggttg acgaactatc
      481 aacctgggcc gcaacaggcg agcccgtgga accgccacag ggtcaacaaa atctacctcc
      541 ggcaggaact ggtggtggtc ctatgagacc ggtttaaaga ccaaaaactg acaaagggat
      601 tggcgcattt gagtatgaaa agataacggg aatgggaacg agacaaccag ccggaatggg
      661 ctttgtgaat aacggggcac gaggaaccaa agattttagg ttcggtgagg acgtactggc
      721 gcgttctgaa gttgcaaaat ttctcaacgg aaaaccgctg acttacgaaa cggctgaaca
      781 attcaaagta tttgtttccg gacatggcgc atttaattct gcagatcagg aaatgatcgc
      841 tgcggccaat ccatatctcc aacccgaggt tttggacaaa ttcatttata aagaaccgcc
      901 tgcccatccg ccgcagaaaa cagcagaacc gctaattcag aatttggaaa agatcacgcg
      961 tgaagatggc tctttacctc tgtattgggc tcagaagatt gccaaggatg ccgaacaaaa
     1021 agttcttaac ggagagaatt tttccgtaga agaccgtaaa atgctcgaaa ctttgcttga
     1081 gcacaaaatt catcagattc cggcttataa gaaaccgttg gaagaggctt taagattggt
     1141 taatcaaggg cttgagcgcc aagccaatcc aaaagcaaaa accggtcctt ctgaaaaacc
     1201 aacagcttct caagaacgtc aacaggaaac aacgcaggat ttcggtgcaa aaacatttcc
     1261 ggccaaaccg ccgccggtta taaaggcaga acctttgccg ccagctggtg gacaaaccaa
     1321 gcctcaaact caacccgtgc cgccaacgga gggaaaccag accgggattt tggcacgtat
     1381 cagtaaaatt gacggtccgc gtgattattt acgggattta gattccaatc aggtcaaaga
     1441 aatgcagcgt gttctaggcg tgaatgatga cggcaagtgg ggacaaaaaa ccatggctgc
     1501 agccgcgcaa tatattgcaa agaataattt gccgcaaaat ataagcctcg aagatctgca
     1561 aaaacatatg cagggtgtta aacctgtatc agaggcacaa caaggctatg atgaagacag
     1621 ggcaacgcat tatgcacaag aggcggcaaa aatagaaaaa ggtgcaacga aagaagaatt
     1681 taaggaagcc cacggcaatc ccgtgccgga cgcagcgccg ggagaaccga aagcaacacc
     1741 gtcaaccact cccgttgtgc ggctcgccta atccagtttc atgacatgta aggtgaaatc
     1801 aagcttttgg ccgagcgccc ggtaaaggct ttgcgcggcg gcattattgc tttcggccag
     1861 ccaatagatg cgcgcccatt tttcgcgctt gccgatctgg ctgatgtagg taatcaattt
     1921 tttggcgatg ccttcccggc gtgcgtcagg ggagacgaaa acatcctgca tataacatac
     1981 gtcatgcaag ctaccggtgg tgggatggag gatgtaatga acaaggccaa caagcgcgcc
     2041 gtctttttcc gcgaccagtc cgcagacttt ggaattcgga tcgatcaggc ggctccaggt
     2101 ctctgatgtc accgcttcat cacgcatgcc gagattgttg ccattccaca gcggcagcca
     2161 gtcaggaaaa tcacggtttt ctatggcgcg gatttttaca gccataatgg gccagccctt
     2221 tatgaacgtg tggaattatt aggccggtcc agcggatcaa agccataatg cgcaagccag
     2281 cgcgtatcgc attcaaaaaa ggtgcgcaag tcaggaatgc cgtatttgag catcgtcagg
     2341 cgttcaatgc ccatgccgaa cgcaaagcct tgataaacat ccgcatccac gctgcagttt
     2401 ttcaggacat tggggtgcac cataccggcg cccagcactt ccatccattt ttgttcttgc
     2461 ccatcggggg ctttgccgag cttaattcca ccatcgggct ttttgatata gccgatatcg
     2521 acttccgcac ccggttccac aaacgggaag aaggatggac ggaatcgcac cggtagatca
     2581 tccattttga aatagctgcg cacgaaatca gtcaggcagc ctttcagatg cgccatggta
     2641 acgtttttgt cgatatacag cccttccatc tggtggaaca tcggtgagtg ggtcatgtcg
     2701 taatcggaac ggtaggtccg cccaaaacac acgatcttat agggtggttc attatctttc
     2761 atatggcgga tctgcacggc ggacgtatgg gtacgcaaaa gttttttggc caactcacct
     2821 tcttcgctcg ggtcttcggg caggaagaat gtatcttgca tttcgcgggc cgggtggcca
     2881 ggcgggaagt tcagcgccgt aaaattatgc cagtcatctt cgatatccgg gccttcggca
     2941 acgctaaagc ccatggcccc gaaaataccg accatttctt ccatagtttg cgaaatcggg
     3001 tggattttgc cgctgcgttc agggcgggtc gataaggtca catcgagcgt ctcggtggcc
     3061 agacgttcat cgagcgcttg tttcttcagg gccttttcct gacgtttaat caactcggcg
     3121 atttcgtcct tcagaacgtt gagcgcggcg ccgacggttt tacgctcttc cgggtccata
     3181 ccgcccagcg tcttcatcag cgcggtaatc cgcccctttt tgcccagtac ggtgacacgc
     3241 acatcatcca gcgctgcagg ggttctggcc gcttcgattt cactggtgat ttcagttttt
     3301 aatgcattga tatcagtcat gaggtgcttt ttagcaccaa ggtcttgatg cgccaagggt
     3361 tttatttatt gacatattgt tacacatgtt gtctattcgt cccacaattc aaagctggag
     3421 taaaaaatga gtaaaacaaa agtcgcagca aatcattatc tggcagaagt ttcacaatct
     3481 gttgacggcg gcggaaggct tggccgggat ttctgggtcg cggtgcgaga ttttaatagg
     3541 tatgagccaa gcgacataac cctttctcaa tactcccagc aataccacac ccccagcaat
     3601 gaacaagttc ggcgcaaaca aaaacgcgct gagcggcttg gggaattata cccgcttgcc
     3661 ggagaggcct gggctcaaga agcatatcgc aatgtgcacg gtttctttaa gcgtttagaa
     3721 gaagagcgct tcaccgaagc ccaagcccag gatttagaaa cagacctgag agaaggcgtc
     3781 caatccattg tccaggacct tcttatcccg cacttaaaat aacttcacga tttcatcgag
     3841 ccggtgaacg atgtgatcgg gggcggcctg ccgcaagcgc gcacgcgaaa tatagccatc
     3901 ggtaatcccg attgaggtaa tccccagcgc ccgcgcaatc tccggttctt ccattgtatc
     3961 gccgataatg attgtgtttt ccggagcatg gccggattcg ctcatataat cgcgcaggcg
     4021 caagactttg gttgtacttt gcaaaatcgt cgtgccgttg caatcatgcg cggaaacata
     4081 agaaaaatag tgttcaatat tcaggcgctt cagatggcgt tcaatccgct cggtaatgta
     4141 attggacaaa ataatgcatt ccgcaccctg ttcgctcaga tactccagca aatcccgcgc
     4201 gccgtggcgg gtgcgtgcac ctgcggccag ttgctcatag gtgtcctgaa acaccgcatt
     4261 gccttcatcg cggcgctcca gcacggtttc ttccgaaacg ccgtgtttct cataaaacgg
     4321 aatgatcgga aagcaaaagg tctcgcggtg ggttttgagc gagatggctt ccgcgccgta
     4381 gaattcgagg cagacattgc ctgccttcca gcttggcacc gtgtcagcca gcagcgtccc
     4441 attccaatca aatacaaaga gtttgttatt gagaatcatg ctccacatcg tacacgacag
     4501 acgccgtagt ctaaaaatga tttctatagt caatcaccgt ttgactcctg aacaaaaaca
     4561 ttgattttca gggcgttttt tcaaagtttc gaaacgattt ttcgtgcgac aaaaaaatat
     4621 aaaggcgcag gcaggaaata taggatcttc atcatccagg taaaacgttt gggaaaatga
     4681 atttcaaagc gctttgaacg caaatctccg gcaatcacct tggccgccgc atcggcttca
     4741 atcatcatcg gcatgggaaa atcgttttta tcagtcagcg gcgtgcgcac aaaaccagga
     4801 ttgataatct tcacatcaat attgtgaggc tccagttcaa ttttaaggct ctcggcataa
     4861 ttaatcatcg ccgccttggt tgcgcaataa ggctgaccat agggcagccc gcgataacca
     4921 gccacgctgc cacataaggc gatctgcccg tggccttgtt tctcaaaaat cggcaaggtg
     4981 gcattggtgg tattatacgc gccgcccaga ttgaccctga tcatgtcgtg aatgaaatcg
     5041 atgtctttag tctctttacc gtgcggcgcg tatagtgcgg ccataaagat aacgcgatca
     5101 agctttggaa tggccttcgc tgcagcgctt aacgccttat gatcgccggc atctaaagcg
     5161 ataacctgat gccccccgcc aagcgcatca ttgaggctat ttaaagcatc ttcattgcgg
     5221 gcagataaaa taagcgctcc gccccgcgct gccagttcaa tagccaaagc ccggccaata
     5281 ccgctgctgg cgcctataat ccaaatatgc tctttgctat attcgcccat aaaaaagctt
     5341 atctaaaaat gactaataaa gcccgccagt gccgacattg gtttcgcttg gcgttgttgg
     5401 cgagggattg taaggtggcc tgcttggcag ctgatccggg ttttgccctg ctgctgttcc
     5461 ctcaggcgta taaagtccgc catcctggcc gttgtcctgc ggaaaaggtt caagatcata
     5521 gccgccggga ttacccggac tataagcatc gctctgagcg tcataatcat aataggccgc
     5581 attgccggta aacagattaa tccccgtacc atcgagaatg aacatgtcat gcgtcggctc
     5641 tgtgccgacc gtaaaagctt cccagatcac ctttttatcg ccgatttgcg cccgcgcgcc
     5701 cgtttcggcg ttaatctgca cattgcgaat gccgttaggt atccgaaacg gcaaaattgg
     5761 ctcatcggcc agcgcttctt ccataaagct tttaaacacg ggcaaggcaa cagaggaacc
     5821 tgtttcgcgt tttcccagcg agcgcggctc atcaaaaccg acaaacaccc cggtaaccag
     5881 atcaggggaa aagcccataa accaggtatc tttggattca ttggtcgtcc cggttttacc
     5941 ggccaaaggc cgctccagct ctttgaggcg tacgcctgtc ccgcgctgga caacgccttc
     6001 catgatcgaa acaatctgat aggcgtggcg ctcatcgacc agttgctcac gcggatcagg
     6061 aagttccggc gcttcttgta aatcccagcg aatgagatca ccgcaattgt ggcactgccg
     6121 gtcatcatgg cggaaaatgg tcttgccacg ccggtcctga atgcggtcga taaaggaagg
     6181 ctctatcttt ttcccgccat ttaccaactc gccataggca ctggttaatt ttaacaatgt
     6241 ggtttcacct gcgcccaaag aattggctag cagcggcggc atatcctcat caatgccaaa
     6301 gcgcttcgca taatccacga ttgtgtccat accaagataa tcggcaaggc gcaccgtcat
     6361 caggtttcgt gatttttcaa cccccacccg gataggagtc gggccgtaat attcgtttga
     6421 atagttggtc gggctccaga aattccccgg tcggtcttca atcacaaaag gtgcatctaa
     6481 aaccagcgtg gcgggcgtaa agtcattttc cagcgcagcc agataaacaa acggcttaaa
     6541 cgcggaaccg ggctgacgct gggcctgcgt tgcgcggtta aactccgaac tgccgtaatg
     6601 gtaattccac ccgccctgca tcgccaaaat gcgacctgta tgcgggtcca tggcaatgat
     6661 tgccccttgg accagcggaa tttgctctaa gacataatta tttttgctgc cgtcgattgg
     6721 tgaaacggca ataatgtcgc ccttggagag cacttcatct acagccgtaa tttccggtcc
     6781 cagcgcataa ccttcattca gggacttgcg cgcccatttt acgccgtcaa gcgggagcgt
     6841 ccctttggtc ccgtcttcaa aaccaatctc ggcgcgcgca ctgcctacgt ccaaaacaat
     6901 cgcgattttc catccgtcga taatgccttg cggcgcttta aattcttcaa gcttactttt
     6961 ccattctgcg acattggtca cggttttgag cggcccacgg tagccatgac ggcgatcata
     7021 agccatcaac ccctcgcgca aagcgcgctc ggcaatttcc tgcaacttcg ggtcaatcgt
     7081 ggtgcgcacc gccagcccgc cttgatacag gctgtcctca ccataacgcc cggccagctg
     7141 gcggcgcact tcttcagcaa aatacggtgc attgacccgc ctatcttcat cgcgcgtgat
     7201 tacttccagc ggtgccagtt tggcaagctc tgcctgacct tctgtaataa agccgttatt
     7261 ttccatccgc cggatgacct cgttgcgctg cttaactgcg cgatcatgat ggcgagtagg
     7321 gtgataatta tttggtgctt tcggaagacc cgccagataa gcagcttcag ccacactaag
     7381 ttcatccaga gatttattga aatattgcag tcccgctgcc gctacgccat aagcccgata
     7441 ccctaggaaa atttcgttca ggtaaagctc aaggatctgg tccttggtca tcgcccgctc
     7501 aatgcggctg gctagaatgg cctcgcggat tttgcgttca aattttcgct cattggtgag
     7561 cagaaaattt ttaacgacct gctgtgtgat agttgacccg ccaataacat tgacatcctt
     7621 acccatcaaa tgctggccgt aaacataagc tgcgcgcaca attgccgttg gatcaacgcc
     7681 ttggtgcgta taaaaattct tatcctccgc agcaataaag gcgtttttta ccagatcagg
     7741 aatcttttcg attggcataa atatgcgctt ttcctgcgca aactctgcca tcagccgacc
     7801 atctccggca taaagccgcg taacaatcgg cggctgatat gttttcagct gcttataatc
     7861 cggcaggtcg gaaccataat aattcaggat ataaaggacc cccgcgacac acacaagcaa
     7921 ccccagaaaa ccgagggaaa acaatccgag aaaagcgtac cagatatatt tcatattttt
     7981 aaaaaccaca gataaacacg gatggacaca gatatgtctt tttaaacacc aacaccatcc
     8041 agcgcaccaa aatttttatt tattttttca agtatgctgg gtcaacctaa ccttaaaagt
     8101 ttgaatctgt gttcatctgt ggttaaaaat caggccacgc tgcgcgtaat cggcccgtca
     8161 gctttgccat gaataaactg atcaacataa gcattgcccg aatcatcgag gtcttcaacc
     8221 ggcccggtcc agacaatttt gccttcatgg atcatcgcta cataatcagc aatcgtgcgc
     8281 acagagccca tatcatgggt aatggacagc gccgttgcgc caatatcgtc aacctgttca
     8341 acgataagct ggttaatgac gtcagccata atcgggtcca gaccggttgt cggttcatca
     8401 aagaaaataa tttctgggct tgccgctacg gcgcgcgcca ggcccacgcg cttttgcatg
     8461 ccgcccgata gctccgccgg aaacaggtca gacacatccg ggcgcaaacc gacagcctca
     8521 atcttctgca cggcaatctc acgggcttgc acccggtcca tcccgcgccc ctgaatcagg
     8581 ccaaaggcga cattctccca aatttttaag gaatcaaata atgccgcgcc ttgaaacagc
     8641 ataccaaact tgcgcatcag cgtgtcgcgg tctttgggcg acaagccgac agtttcctgt
     8701 ccgtcaattt caatggagcc gctatccggg gtcatcaatc ccaaaataca tttcagcatc
     8761 accgatttac ccgtgcccga tccgccgata atcaccatgg atttgccttc cggcacgaca
     8821 aggtctacgc cgttaagtac acggttagaa ccaaaggcct tgcagacatt ttttagccca
     8881 atttttggcg gtgtatcgct catgagaaaa acacctgtgt caacagatag ttaaagatca
     8941 aaatcaggat cgaggatgat accaccgcgt tggttgtagc cgcgcccacc ccttgcgccc
     9001 cgcgcccgga attaaatccg ttataacagc ccatcaccgc aacgataaag ccaaacgcac
     9061 ctgccttaac caaaccggac actacatcca tcgtctcaat aatatcccaa gtttgagtaa
     9121 gataagtacc ggggctaaaa cccagattat aaattgaaac aacatagcca ccataaactc
     9181 cgataatatc accaatcagc accaaaattg gtaaagtaat cgtcgcggca atcaaacgcg
     9241 gcgcaatcag atatttaaac ggattaaccg aaagcgtgca cagcgcatca atttgctcag
     9301 tcacgcgcat cgtgccaatt tctgcagcaa tcgccgcgcc cacacgtccc gcaaccatca
     9361 ggcctgccaa aacaggtgcc agctcgcgcg ttaccgaaag caccaccacc ccggcaatcg
     9421 caccctcggc attaaagcgg gtaaagccgg tatagctttg cagcgccagc accatcccgg
     9481 taaacagtgc ggtcatacca actacaggca gcgagtaata accaatatcg accaattgtc
     9541 gccaaagttg ggcggggaaa aatggtggtg taaaactgtg gtaaattgca cgtccaataa
     9601 aaatagataa ggccccggcg cgctgaaaaa aatttaaaat aaacgcaccg accgcctgaa
     9661 agacattcaa taacggatca aaaagacctg atgattgtat agacgattgt ggttccatga
     9721 atagtttctg ttatagttca aaaatgcaca gtaaaacatt gctccgcaat ggtccagcgt
     9781 gaaagcacga ggttaagact tcaatcacat gaaaacactg cacaatataa atccggcaga
     9841 tctggttgat atgagtgcac aggaaagcgc ccatccttac cgcgtggaag tcgcctatgc
     9901 gtgcgatgat aatttattgt ttggccagca gatttaccgc tctgacgcgc atttatggct
     9961 gcatcaggat ttggccgata ttgtttcgaa agccgcagct gacctaaacg atcaagggta
    10021 ttgcctgatc ctctatgacg gattgcgtac ggttgacgca caggcagcca tgcttgaaac
    10081 ccgcgcggta aaagaaaatc cgcattggct ggaagagccg cggctgctct ctccacccgg
    10141 cgcaggcgca cacccgaggg ggatggcgat tgattgttcg cttgaaacgc tggagggccg
    10201 acttttggat atgggtacgc cctttgatta tctcagcacc gatcccgcgc cggaaagcaa
    10261 tcccgcccac cgcgcataca cagatctcac cgaagaggcg cgcacgaacc gtaaaatcct
    10321 tgacactgca atggccaaag ccgcgcaaga atctggaacg ccgctctggc cgctgccgca
    10381 agaatggtgg gattttcgcc tgccgttaga ggtgagcgaa acctacgccc ctctaagcga
    10441 cgccgattta ccgccagaaa tccgcatggt acgctattcc caactttctt cttttcagcg
    10501 aaatacaaaa cttgtgaaac cccgcacttg atgcggggtc caatacgata cataattaaa
    10561 ccctatgggc cccggcttaa agccggggtt tcttttttct ttttatttca atcatttaaa
    10621 agaagttgag aattgggtga tgatcggtta atcaatatag cggcgcaaat gccgcgcccc
    10681 caggctggtg agaatttcat aaccgattgt cccggcatcg cgggccaggt catccgcgct
    10741 ttgatgcatc ccaatcacct ccatcatacc acccggtgtc ggcaaagccc tttccgccac
    10801 agcgctcaaa tcgacaatgg tcagatccat agaaacgcgt ccgcgcacag ggcaaggaat
    10861 gccttgccaa tacaacaccc cgccattcga gagactacgc aaaaacccgt ccgcatagcc
    10921 aagcgcgacc acggccagcc ttgttttatt tttaaaacga tacgtagcgt tatatccgca
    10981 ggtctcgcct ggcgccgtgt cacggatttg caaaatggga accgacaggc tgaccactgc
    11041 ctccatcgga ttttccgttt ccggcttggg gttcagacca tacaaacaca taccagggcg
    11101 aaccatatcg aagtgatacg cattggagcg aaaagccccg gaggaattgg cgagcgattt
    11161 cttcgttcgg ggaaaatgtt ttgcagcggc ttgaaagact tcaaactgcg ccgcgttcat
    11221 tgaatggtct ttttcatccg cgcaggcaaa atggctcatc acaagactga aatcatgccc
    11281 ctgtaacgca tccagcctat ccatgcgaat acccaaacga ttcatccccg tatcaatatg
    11341 cagcgctgga gcagggaggt cctgcccatg cgcaccgaaa tcctgcacat cttcaagtga
    11401 attcaaaacc gggataatct gttcagattg ataaacatga gcgccccgcg catcaaagcc
    11461 attaagcgta taaatcactg gcccttcgcc aagtatgcca cgcagcgccg ctgcctcatc
    11521 cagattggca acgaaaaaat cctgtgctcc cgcacccttt aaagccttgg ccaccgccgc
    11581 gacgcctaag ccataaccat cggccttaac caccgctgcc gccgtgcagt tttcaccaac
    11641 catgttttga attgtacgat aattacgcgc caatgcgcca agatcaatgc tgaggcgccc
    11701 gcgcatcgct tactctccga aactcgcccc gatatgattg gggtcaagat ccgaaaattt
    11761 cgtcaggttc ggatcaaaga acatctttac aatgccgatc gggccgtggc gctgcttggc
    11821 cacaatacat tcgccaacat tatgcgcgcg ttccatgctt tcctgccact tcatatgttt
    11881 ttccgtaccc ggctcaggtt ccgcccgcga tagataatat tcctcgcggt agacaaacat
    11941 caccacatcc gcatcttgct caattgatcc tgattcccga agatccgaga gttgcgggcg
    12001 tttatcttca cgctgttcca ccgcccgtga aagctgcgac agcgcaatca ctggaatttc
    12061 cagctctttg gcaatggcct taagcccccg cgtaatctcg gaaatttcca gcaccctatt
    12121 agattcagac tggcgcgaac ccgtgccgcg caaaagttgc aaataatcca ccacgatcat
    12181 atccagcccg tgctggcgct tgagcctgcg tgaacgcgtt cgcaccgcgc caatcgacag
    12241 ggcaggggta tcatcaatat acagcggcac ctgactcatc ttttgtgaag cttcaacaaa
    12301 ggcgcggaaa tcttcctgct tgatattgcc cttgcgaatc gcatcaccgg aaatgcccga
    12361 ttgctccgac aaaatccgcc cggcaagctg ttccgccgcc atctccaaag agaaaaacgc
    12421 cacgcgcccg ccttgctttc cgccgctttc cgcataggcc ttggccgcat taaacgccca
    12481 gttcaccgcc agcgcggttt tccccatcga cggacgcccg gctaaaatca gcagatcgga
    12541 attctgaaat ccaccgagct gtttatcaac atcgaccagc ccggaggtaa taccggtcac
    12601 atgcccgtcg gactggaagg ctttttccgc atgctcattc gccgtgagaa ccgaatcgcg
    12661 caaggtgata aagccgcctt tgacatcgcc ggtttcagcc agttcgaaca ggcgcgattc
    12721 catgcgttca accgtgctca tcgcatcgga atccagcgaa tgctcatatg cttcgttgac
    12781 gacatcttcg cccagtgaaa tcaactggcg gcgtagataa agttcgtaaa tcgttctggc
    12841 gtaatcggcc gtattaatga cactgaccac attggccgcc aggtcagcca gatatgacgc
    12901 gccgccgaca tcgctcaaat cttcgtcatt ttcgaaatag ttttttagcg ttaccggcga
    12961 agcattttgc ccgcgctcga tcaaggtta
//
LOCUS       DAOSWR010000052        12933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3067_length_12933_cov_5.226200, whole genome shotgun sequence.
ACCESSION   DAOSWR010000052 DAOSWR010000000
VERSION     DAOSWR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_12933_cov_5.226200"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK513_05335"
     CDS             <1..547
                     /locus_tag="PK513_05335"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292843.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HOO81904.1"
                     /translation="VISGGGGILVLEELEHAKARGAKIYGEITGYGATSDGADMVAPS
                     GEGAVRCMRQALSTIKTPVTYINTHGTSTPVGDITEINAIKEVFEPLDQAMPHISATK
                     SMTGHSLGGTGVQEAVYSLLMMKHNFVAPSINIENLDEGAAGLPIARARVDNVQHETI
                     ISNSFGFGGTNCTLAISKYKG"
     gene            554..1354
                     /locus_tag="PK513_05340"
     CDS             554..1354
                     /locus_tag="PK513_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO81905.1"
                     /translation="MHSEFMKGKKGLIMGVANDHSIAWGMAKALHEAGAEMAFTYQGE
                     AFGKRVKPLAESIGSDILIDCDVTSEDNLDEVFSTLADKWGRLDFLVHAIAYSDKNEL
                     QGRYVDTSLDNFLKTMHISCYSFTSVMRRARDLMSDGGSAVTLTYYGAEKVMPNYNVM
                     GVAKAALEASTRYLAADLGPENIRVNAISAGPMRTLAGAVIGGSRKTFKYCNAAAPLR
                     RPVELDELGGTALYLLSDLSSAVTGETHYVDCGFNAMGMAPHDQLDQL"
     gene            complement(1440..2186)
                     /locus_tag="PK513_05345"
     CDS             complement(1440..2186)
                     /locus_tag="PK513_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOO81906.1"
                     /translation="MPLKLPKIIGHRGCAAYAPENTIEGIHTAADMGVEWVELDVKLT
                     KDQVPIIFHDETLERTTDSSGNIRDLTLQEVKELDAGSWFSDGFFGVSVPTLEEAVDA
                     LIERDLGLNLEIKPCPGREKETAEVALDVLSGIWDDHDRLLISSFQHVSMETALDMAA
                     DWYRGFLLPEAWPENWRELAEYLQVSTVHLNGNTCTREQVDMAMELEKPVLAYTINDT
                     DRARFLQSWGVDGFFSDAPDVLQDGLFSVH"
     gene            complement(2294..4417)
                     /gene="pnp"
                     /locus_tag="PK513_05350"
     CDS             complement(2294..4417)
                     /gene="pnp"
                     /locus_tag="PK513_05350"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO81907.1"
                     /translation="MFKVYRKEIDFAGKILTLETGKIARQADGSVLATIGETSVLCCV
                     TAAKSVKEGQDFFPLSVHYQEKTYAAGKIPGGFFKREARPSEKETLTSRLIDRPIRPL
                     FPKGFLNEVQVVATVISHDLENDPDMIAMVGCSAALTLSGIPFMGPIGGARVCYKDGE
                     YLINPPMPEVGETDLDLVVAGTGEGVLMVESEAQELSEEVMLGAVSAGHKAYQSVISG
                     IIELAEMAAKDPWDIEETPEAIKKLEADIKAKFAADVEKAYGITDKLERQTAVGEVRN
                     AAIEAFTDEENGINDKIITAKFKSLEADVVRGSILKTGKRIDERDTKAVRPIVAEVGV
                     LPRTHGSALFTRGETQALVVSTLGTGQDEQIMDTLEGEYRERFLLHYNFPPYSVGEAG
                     RMGPPGRREIGHGKLAWRAIHPLLPSAEDFPYTMRSVSEITESNGSSSMATVCGTSLA
                     LMDAGIPLKAPVAGIAMGLIKEGDEFAVLSDILGDEDHLGDMDFKVAGTENGITALQM
                     DIKITSITEEIMTIALAQAKDGRIHILGEMAKALAEARAELNEHAPQIVSLKIPVDKI
                     RDVIGTGGKVIREITETTGVKMDVEDDGTIKVAAANQESIDAAVKWIKDLTDEPEVGE
                     VYTGKVVKTVDFGAFVNFMGAKDGLVHISELQDARTANTTDVVNEGDEVKVLLMGIDD
                     RGKIKLSMKRVDQETGEKIEKEDAA"
     gene            4749..5387
                     /locus_tag="PK513_05355"
     CDS             4749..5387
                     /locus_tag="PK513_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOO81908.1"
                     /translation="MSNTLVLLRHGQSQWNLENKFTGFHDVDLSGKGIEEARQAGEQI
                     KQAGITFDQVFTSTLTRANRTAKLALEAAGQSDLIDTMTFHDDLRERDYGDLTGLNKD
                     ETRAKYGDEQVHIWRRSYDTRPPGGECLQDVVENRVRPYYEANIKPLLDDGKNILIAA
                     HGNSLRAMLIILGVETPDSINNAEIPTGQPLVFELDNGEIKARYFLSEKEVA"
     gene            complement(5391..5879)
                     /locus_tag="PK513_05360"
     CDS             complement(5391..5879)
                     /locus_tag="PK513_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81909.1"
                     /translation="MNTPPLVKVFALYSTLTFLDGCSTPRQPNGGPLIFGPIPVQPTI
                     QSWIHGNDEPKDDCERDVVVQAQGWLDKYGQEMDENITFYSPLDEIKSVWDAKKFLSD
                     LEIYLAPVNATMASRKPSDYADVRELNRRIHYNNRFEPARVIQKALGAKGPSFEHFNC
                     RW"
     gene            complement(5985..6263)
                     /gene="rpsO"
                     /locus_tag="PK513_05365"
     CDS             complement(5985..6263)
                     /gene="rpsO"
                     /locus_tag="PK513_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012179655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO81910.1"
                     /translation="MSISANIAEKKAKTIEEYATKKGDTGSPEVQVAILTTRINELSE
                     HMQQHKKDFHSRHGLLAMVAKRRKLLDYLKGKNEARYQDLIKRLGIRR"
     gene            complement(6278..7201)
                     /gene="truB"
                     /locus_tag="PK513_05370"
     CDS             complement(6278..7201)
                     /gene="truB"
                     /locus_tag="PK513_05370"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOO81911.1"
                     /translation="MGRRKKGDKIDGWVNLDKPLGLTSTQAMAKVRRILNAQKAGHAG
                     TLDPSATGILPIALGEATKTIPFAQDRLKIYEFTITWGEQRDTDDSEGETIATSDVRP
                     APADIEALLCEFMGEIEQIPPRFSAIKIDGQRAYDLARGGEDVELKPRKVFIESLELL
                     DARADDADFVMTCGKGTYVRSLARDMAEKLGTYGYVSALRRLKVGPFSEKNAISLEKL
                     EEMGDKAREALLPLETVLDDIPALAIKEGEMAKFRNGQSVSFVARPDFERLKQIGLEN
                     DHIAIAMFQERPIAMVERQGPNIQPVRVFNI"
     gene            complement(7205..7588)
                     /gene="rbfA"
                     /locus_tag="PK513_05375"
     CDS             complement(7205..7588)
                     /gene="rbfA"
                     /locus_tag="PK513_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOO81912.1"
                     /translation="MSSHHTHKGPSQRQLRVGEQLRHIIAETMSRGHFHHEALLDAGR
                     VSVTEVRVTPDLKNAKAYVMSLGGADMDIILPALNEEAHVFQKEIGRQQNMKFTPKIR
                     FVRDESFDEAQRIDDLLRGVHLPEE"
     gene            complement(7604..10099)
                     /gene="infB"
                     /locus_tag="PK513_05380"
     CDS             complement(7604..10099)
                     /gene="infB"
                     /locus_tag="PK513_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO81913.1"
                     /translation="MSETKNKDSDDQKKEGGKKTLSLGGTLSLKGGAPTPRPGIAPGT
                     VAVEVRRKRTTAPTSAPQDNTPQSDAHLSDAEREARAKALEKALADGGKTKSSLPKRR
                     TTIEDKKKEEAANKEQAEKSAREKELEELERIEAEESKKAADAQANAPTIPLNPDDPS
                     TARRAKFGEDSTSENYRDKLKKAAPKAPSKTAEDRRGGRLTVTQALNRDFDRDRGMSM
                     AAQRRAREKARMAAKGPKEPAQKVFREVVVPEVITVGELANRMTEPTGEVVKTLMKLG
                     VMATVTQSIDADTAELVIGEFGHTIKRVTEADVEEGLSGVEDKPENLKTRPPVVTIMG
                     HVDHGKTSLLDAMRQTNVVSGEAGGITQHIGAYQVHMESGEKITFLDTPGHAAFTEMR
                     ARGANVTDICVIVVSAEDSVMPQTIEAINHAKAAKVPIIIAVNKIDLPAADPMKVKTE
                     LLQHEVVVEDLSGDVQCVEVSAKQKTNLDKLEEAILLQAEVLELKANPNREAQGVVIE
                     SKLEKGRGPVVTVLVQNGTLHIGDVFVAGAEFGKVRALINDQGKNVDEAIPGQPVEVL
                     GLNGVPDSGDLVSVTPDEGKAREISEYRQRKRREMQAVAAVKGRTTIDDLLAAREAGE
                     KTALPVIVKGDVHGSVEAILGSFDKMVAENDDIEVQALHTGVGGITESDVKLARASGG
                     MIIGFNVRANVQARDLAQREGVDIRYYNVIYDVIDDAKAILSGMLSPSQREEYIGNAT
                     ILQVFNVTKVGKVAGCMVDMGIVKRGAGVRLLRDDVVIHEGKLKTLQRFKDEVKEVKE
                     GTECGMAFENYDDIKAGDVIECFEIIEEARTVQ"
     gene            complement(10320..11864)
                     /gene="nusA"
                     /locus_tag="PK513_05385"
     CDS             complement(10320..11864)
                     /gene="nusA"
                     /locus_tag="PK513_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOO81914.1"
                     /translation="MELLQVADNVAREKNIDREIVVTAMEEAIQKAGRSKYGHDHDIR
                     AEINRKTGNIALKRYREVVEETAEIENEMAQMPLSQAQRIKPDAKIGEFLIDELPPLD
                     FGRIAAQTAKQVIMQKVREAERSRQYSEFKDRVGEVINGVVKRVEYGNATVDIQGRAE
                     AVLRRDEGLPREHLKNGDRVRAIIYEVREEMRGPQIFLSRTHPEFMAKLFMQEVPEIY
                     DGIIEIKSVARDPGSRAKIAVVSKEGSIDPVGACVGMRGSRVQAVVNELGGEKIDIVP
                     WSEDIAGFVVNALQPAEVTKVVLDEETKRMEVVVPDEQLSLAIGRRGQNVRLASILVG
                     WDIDILTEAEESERRAEEFKTRSSLFMEALDVDDVIAHLLVAEGFTKVEEIVETPIDE
                     LNQIEGFEEEISTELQNRAATWLDAKQKELSEKQVELGIADDLMHAEGLTPAQIIMLG
                     KTGVKTLDDLADLAADELAETLGEGQITVKDANAVIMAARAHWFEAEDKVAAEAESAA
                     AAENAA"
     gene            complement(11877..12500)
                     /gene="rimP"
                     /locus_tag="PK513_05390"
     CDS             complement(11877..12500)
                     /gene="rimP"
                     /locus_tag="PK513_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOO81915.1"
                     /translation="MLGFLNLMRHCERSEAIQSHKAKILDCRVSPSGFLAMTVRIVMK
                     LTPLEQRLSEYARPVIEDLGFALVLIKVVGEGGSQNVQILAEDPATRNLGVDDAAVIS
                     RALSAVFDVEDPIKGAYRLEVSSPGIDRPLVRIEDFETYKGFEAKIESCVPAENGQKR
                     FRGVLQGLNGESVLIDTDQGKAEIPFSNLTKAKLVMSDELIQKTANL"
     gene            complement(12598..>12933)
                     /locus_tag="PK513_05395"
     CDS             complement(12598..>12933)
                     /locus_tag="PK513_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81916.1"
                     /translation="ALKNTRLSKVIFNTYQSKISSVENITLEHRTPEGRLIPPLKNNL
                     KEFKSCIIGANQICYYEQVYNDSNLPHDMIENVIIDFTANGKKHHISKTYLIERAYHF
                     SFWDILMGV"
BASE COUNT         3028 a         3658 c         2889 g         3358 t
ORIGIN      
        1 cgtcatctcc ggcggcggtg gcattctcgt tctcgaagaa ctcgaacatg ccaaagcgcg
       61 cggggcaaaa atctatggcg aaatcaccgg ctatggcgca acatccgacg gcgccgacat
      121 ggtcgcgccg agcggggaag gggcggtgcg ctgcatgcgc caggcgctgt ccaccatcaa
      181 aacgccagtg acctatatca atacgcacgg cacctcgacg cctgtgggtg atatcaccga
      241 aatcaatgcg atcaaagaag ttttcgaacc gctcgatcaa gccatgccgc atatcagcgc
      301 cacaaaatcg atgaccggcc actctctggg cggtaccggc gtacaggaag cagtctattc
      361 gctactgatg atgaaacata atttcgttgc cccgtccatc aacatcgaaa atcttgacga
      421 aggcgccgcc ggactcccca tcgcccgcgc gcgcgtcgat aacgtccagc acgaaacaat
      481 aatttccaac agctttggct tcggcggcac caactgcacc ctcgccatca gtaagtacaa
      541 agggtagggc gcaatgcatt cagaattcat gaaaggcaaa aaagggctca tcatgggcgt
      601 ggccaatgat cactccatcg cctggggcat ggccaaggcc ttgcacgaag cgggcgcgga
      661 aatggctttt acctatcaag gcgaagcctt tggcaagcgc gtgaaaccac tcgctgaaag
      721 cattggttcc gatatcctga tcgattgcga cgtaactagt gaggataacc tcgatgaagt
      781 tttttccaca cttgcggata aatggggcag gctcgacttt cttgtccacg ccatcgccta
      841 ttctgataag aatgagctgc agggccgcta tgtggatacc tcgctggata actttctgaa
      901 aaccatgcat atctcctgct attctttcac ctctgtcatg cgccgggcca gggatttgat
      961 gagcgatggc ggcagcgcag tgacactcac ctattacggt gcggaaaaag tcatgccgaa
     1021 ttataacgtt atgggcgtgg ccaaagccgc gttggaagcc tcaacccgct atctggcggc
     1081 cgatctcggc cctgaaaaca tccgcgtcaa tgcgatcagc gccgggccga tgcgcacatt
     1141 ggcgggcgct gtcattggcg gctcccgcaa aaccttcaaa tattgcaacg ccgcagcgcc
     1201 cttgcgaaga cccgtagaac tcgatgaact gggaggaacc gccctgtatt tgctctcaga
     1261 cctgtcgagc gctgtcaccg gtgaaaccca ctatgttgat tgcggcttca acgccatggg
     1321 catggccccg cacgatcagt tggatcaact ttaacgtcat tgcgaggagc aaagcgacga
     1381 agcaatctac tctattgtga aaatggattg cttcgctgtg ctcacaatgg caaaaaagcc
     1441 taatgcacgc taaaaagccc gtcctgcagg acatcaggcg cgtcggagaa aaaaccgtcc
     1501 acgccccagc tttgcaggaa tcgcgcccgg tccgtgtcgt tgatcgtata ggctagcacc
     1561 ggcttttcca attccattgc catatcaact tgttcacggg tgcaggtgtt accgttcaaa
     1621 tgtacggtcg aaacctgcaa atattcggcc agctcccgcc agttttccgg ccacgcctca
     1681 ggcaacaaaa acccgcgata ccagtccgca gccatatcca gcgcagtttc catgctcaca
     1741 tgctggaatg acgaaatcaa caacctatca tggtcatccc aaatgccgga aaggacatcg
     1801 agcgccactt ccgccgtttc tttttcacgc ccggggcagg gcttgatctc taaattcagt
     1861 cccaaatccc gttcaatcaa cgcatcgaca gcctcttcca gagtcggcac gctcacacca
     1921 aaaaaaccat cggaaaacca gcttccagca tccagttcct taacttcctg cagggtcaaa
     1981 tcacggatat tcccgctgct atccgtcgtg cgttccagcg tttcatcatg gaagataatc
     2041 ggcacctgat ccttggtcag cttcacatcc agctcaaccc attccacgcc catatccgcc
     2101 gccgtatgaa tgccttcaat cgtgttttcc ggcgcatagg ccgcgcaccc gcggtgacca
     2161 ataatttttg ggagttttag gggcatagat cctcatcatt gtcattgcga ggaaggccga
     2221 agccctgacg cggcaatcca actcctgtag atggattgct tcgctccgct cgcaggtgac
     2281 gtgtcctcag tgattaagcg gcatcttctt tctcgatttt ctcgccagtc tcctgatcaa
     2341 cgcgtttcat ggaaagcttg atcttgccgc gatcatcaat gcccatgagc agaaccttga
     2401 cctcgtcacc ttcattcact acatcggtgg tgttagcggt gcgggcatcc tgcaattcag
     2461 agatatgaac cagaccatcc ttcgcgccca taaagtttac aaaggccccg aaatcaacgg
     2521 tttttacgac tttgccggta tagacttcgc ccacttccgg ctcgtccgtt aggtctttga
     2581 tccacttcac agccgcatca atggattcct gattagcagc agcaactttg atcgtgccgt
     2641 catcttccac atccatttta acgcccgtgg tttcagtgat ttcacgaatg accttaccgc
     2701 ctgtgccgat cacatcgcgg attttatcaa ctgggatttt cagcgataca atctgcggtg
     2761 catgctcgtt cagctcggcg cgtgcttcgg ccaacgcttt cgccatttca cccagaatat
     2821 ggatacggcc atctttcgcc tgcgccaggg caatcgtcat gatttcctcg gtgatagacg
     2881 taatcttgat gtccatctgc aaagcggtaa tcccgttctc ggttccagcg actttgaagt
     2941 ccatatcacc taagtgatct tcatcaccaa ggatgtcaga cagaactgca aactcatcgc
     3001 cttctttaat caatcccatc gcaatccccg cgacaggagc cttcaacggt atgcccgcat
     3061 ccataagtgc taaagacgtt ccgcaaacag ttgccataga ggacgaacca ttactttcag
     3121 taatctcgga tacgctgcgc atcgtgtaag gaaaatcctc agcagacgga agcaggggat
     3181 gtatagcgcg ccatgccagt ttcccatgac cgatttcacg tcggcctgga ggccccatac
     3241 ggccggcctc accaacggaa tatggcggga agttataatg cagcaagaaa cgctcgcgat
     3301 attcaccttc caacgtgtcc atgatttgct cgtcctgacc ggttccaaga gtggaaacaa
     3361 ccaaagcttg cgtttctccg cgcgtaaaca gagcagagcc gtgcgtacgc ggcaaaacgc
     3421 caacttccgc aacaatcggg cgcacagcct ttgtgtcgcg ctcatcaata cgcttgcctg
     3481 tttttaggat cgagccgcgc acaacatcgg cttccagaga tttgaacttg gcggtgatga
     3541 ttttgtcatt aatgccgttt tcctcatccg taaaggcttc aatcgccgca ttgcggactt
     3601 cgccaacagc cgtttggcgc tcaagcttgt cggtaatgcc ataggctttt tcaacatccg
     3661 ccgcaaactt ggctttaata tcggcttcca acttcttgat cgcttccggt gtttcctcga
     3721 tatcccacgg atctttcgcg gccatttccg ccagctcaat aataccgcta atcacagact
     3781 gataagcctt atgaccggcg ctcaccgcgc caagcatcac ctcttcggaa agctcctgcg
     3841 cttcggattc aaccatcaac acgccttcac ccgtaccggc aacaaccaaa tccagatccg
     3901 tctcgccaac ctcaggcatc ggcgggttga tcagatattc accgtcttta taacaaacgc
     3961 gcgcaccgcc aatcggtccc ataaacggaa tgcccgagag tgtaagtgcg gcagagcacc
     4021 ccaccatggc aatcatgtct ggatcgtttt ccagatcgtg agaaatcacc gtcgccacaa
     4081 cctgcacctc gttgaggaag cctttaggga acagcggacg aatcggacgg tcgattaagc
     4141 gtgatgttag cgtttctttt tcgctagggc gcgcctcacg tttgaaaaag ccgcccggaa
     4201 ttttaccggc ggcatatgtt ttttcctgat agtgaacgga aagcgggaag aagtcctgcc
     4261 cttcttttac gcttttggct gccgttacgc agcataaaac ggatgtttcg ccaatggtgg
     4321 caagtacgct gccatcggct tgacgggcga tttttcctgt ttccagagtg agtatttttc
     4381 ctgcaaaatc aatttctttg cgatagactt taaacatgta ttttaccttt catataaccg
     4441 caaacgcatc tccggcgtta cggtttcatt gatgtttaaa attttgggaa tttgtggggt
     4501 ggactgtaaa atgcaaacgt ttgcacttaa cggtcaacct cacgcttccc ccttttcaat
     4561 tgcgcgtgag agtggcatga tccgctctga aagtcaaaga taaaaggcaa gccgtgttta
     4621 tattagaggt gtttctttcg atcattatac tgattttggc aatacctttg gcagcctttt
     4681 tactttttat attgaatcct gaattatgga tttttgattt tatttacccc caaagaaagt
     4741 caataaaaat gtccaataca ctcgtccttc tccgccatgg ccaatcacaa tggaaccttg
     4801 aaaacaaatt caccggattt cacgatgtag atttgtccgg caaaggcatc gaagaagccc
     4861 gccaggccgg cgaacagatc aagcaggccg gcattacctt cgatcaggtg tttaccagca
     4921 cgctgacccg cgccaaccgc acagccaagc tggccttgga agcagccggg cagagcgatt
     4981 taatcgacac catgactttt catgatgatc tgcgcgaacg tgattacggc gatctcaccg
     5041 ggctaaataa ggacgaaacc cgcgccaaat acggcgatga acaggtccat atctggcgtc
     5101 gttcatatga cacgcgtccg ccgggcggcg aatgtctgca agatgtggtc gaaaaccgtg
     5161 ttcgcccgta ctatgaggcc aatatcaagc cgctgctcga tgacggaaaa aacatcctta
     5221 ttgccgccca tggtaattct ttgcgcgcca tgctgatcat tctcggcgtc gaaacgcccg
     5281 atagcatcaa caatgccgaa attccaaccg gccagccttt ggttttcgaa ctggataacg
     5341 gcgagatcaa agcccgttat tttctcagtg aaaaagaggt ggcttaaagt ttaccagcgg
     5401 caattgaaat gctcgaatga agggcctttc gcgcccaaag ctttttgaat aacgcgcgca
     5461 ggttcgaatc gattattata gtgaatcctg cggttaagtt cgcgcacatc cgcataatct
     5521 gaaggctttc gtgaagccat agtcgcgttt accggcgcaa gataaatctc aagatcggat
     5581 aaaaatttct tagcgtccca aacacttttg atctcgtcca gtgggctgta gaatgtgatg
     5641 ttttcatcca tctcctggcc atatttatca agccaccctt gcgcttgcac taccacatcc
     5701 cgttcgcagt catctttagg ttcatcattt ccatgaatcc aggattgtat agtcggctga
     5761 acgggaattg gtccaaaaat aagcggcccg ccatttggtt gtctaggggt gctgcatcca
     5821 tccaaaaacg ttagagtaga atacagggca aaaactttaa ctaaaggtgg tgtattcatg
     5881 ataccctcca aatttgccat aacgtacacc taccgcctta cagaatgcaa gaaaaaagcg
     5941 ctttccgcac gaaaagcgct ctttcaaatc tatatatccg tgttttagcg acggataccc
     6001 aggcgtttaa tcaaatcctg ataccgcgct tcgttcttac ctttcaggta atccaacaac
     6061 ttacggcgct tggcgaccat ggccagcaga ccgtggcggc tgtggaagtc ttttttgtgc
     6121 tgctgcatat gttcagacag ctcgttaata cgggtagtca gaatagcaac ctgaacttca
     6181 ggtgagcctg tgtcaccttt tttcgtggca tattcttcaa tggtttttgc ttttttctcg
     6241 gcaatattgg cactaatcga catcgtcttc tccttcgtta aatgttgaaa acgcgaaccg
     6301 gctgtatatt gggtccctga cgctcaacca tagcaatcgg acgttcttga aacatcgcaa
     6361 tcgctatatg gtcgttttcc agcccgattt gtttcaatcg ttcaaaatca ggtctggcga
     6421 caaaggacac agactgtccg ttacgaaact tcgccatttc gccctcttta atggccagag
     6481 ccgggatgtc gtccagcacg gtctcaagag gaagcaaagc ttcgcgcgct ttatccccca
     6541 tttcttctaa cttttcaagg gaaatcgcat ttttctcgct aaaagggccg actttcaagc
     6601 gccgcagtgc cgaaacatag ccgtacgtgc ccagcttttc agccatatca cgcgccagcg
     6661 agcgtacata ggtgcctttt ccgcatgtca tcacgaaatc ggcgtcatcc gctcgcgcat
     6721 caagcaactc aaggctctca ataaagacct tacggggctt gagctcaacg tcttccccgc
     6781 ccctcgccag atcataggcc cgctgcccgt caatctttat ggcagaaaac cgcggcggaa
     6841 tttgctcaat ttcacccata aattcacaga gcaaagcctc aatatccgca ggggcagggc
     6901 gcacatctga agttgctatc gtttcacctt ctgagtcatc cgtgtcgcgc tgttcccccc
     6961 atgtgatggt aaattcatag atttttagac ggtcctgcgc aaaaggaatg gtcttggtag
     7021 cctcacccag cgcaatcggc aaaatcccgg tagccgacgg gtcgagcgtt ccggcatgcc
     7081 cggccttttg cgcattgaga attcgcctca ccttggccat cgcttgcgtg gaagtcaacc
     7141 ccaggggctt gtcgagattg acccacccgt caatcttatc gccttttttc cgccgcccca
     7201 tgatttattc ctcgggaaga tgcacgccgc gcagcagatc atcaatgcgc tgtgcttcat
     7261 caaagctttc gtctctaaca aaacgtatct tgggcgtgaa tttcatattt tgctgtcgtc
     7321 caatttcttt ttgaaaaaca tgagcttctt catttagggc gggcagaata atatccatat
     7381 ctgcaccacc taaagacatc acataggctt ttgcgttctt caaatccggc gttactcgca
     7441 cttcggtcac gcttacccgc cccgcatcca gcaaggcctc atgatgaaaa tgtccgcggc
     7501 tcatcgtctc ggcaataata tggcgcaact gttctccaac gcgtaattgc cgttgcgacg
     7561 gccctttatg tgtgtgatgc gaactcatag ctatattgac cgactattga accgtacggg
     7621 cttcttcaat gatttcaaag cattcgataa catcgcccgc cttgatgtcg tcgtaatttt
     7681 cgaacgccat cccgcattca gtgccttctt taacctcttt gacctcgtcc ttaaagcgct
     7741 ggagggtttt tagcttgcct tcatggatca ccacatcatc gcgcagcaga cggacgcccg
     7801 ccccgcgctt aacgatgccc atatccacca tacatccagc cacttttccg accttggtga
     7861 cattaaacac ctgcaggatt gtggcattgc cgatgtattc ttcgcgttgt gacggtgaca
     7921 gcatacctga caggatcgcc ttggcatcgt cgatcacatc gtaaatcaca ttgtaatagc
     7981 gaatatccac gccctcgcgc tgagctagat cccgcgcctg cacattggcc cgcacgttaa
     8041 agccgataat cattccgcca gatgcccgcg ccagcttcac atcgctctcg gtaatcccgc
     8101 caacgccggt atgcagcgcc tgcacctcaa tatcgtcgtt ctctgcaacc attttatcga
     8161 aggaccccaa aatcgcttcg acagagccat gcacatcccc cttaacaatg acgggcaggg
     8221 cggttttttc accagcttca cgtgcagcta gtagatcatc aatcgttgta cgccctttga
     8281 cagcggcaac agcttgcatc tcacggcgct tgcgctggcg atactcgctg atttcacgtg
     8341 ctttaccttc atcaggcgtt acacttacca gatcaccgga atcaggcaca ccattcagac
     8401 cgagaacctc aaccggctgt cccggaatgg cctcgtcaac atttttaccc tgatcattga
     8461 tcaacgcccg cactttgcca aattctgcac ccgcaacaaa gacatcgcca atatgcagtg
     8521 ttccgttttg cacaagcaca gtcacaactg gtccacggcc tttttccagc ttggattcta
     8581 taactacacc ctgtgcttcg cgattcgggt tggctttaag ttccagcact tcagcttgca
     8641 gcaagatcgc ttcttccagc ttatccagat tggttttctg cttggcagag acctctacac
     8701 attgcacatc cccgctcagg tcttcgacaa ccacttcatg ctgcaacaat tcggtcttca
     8761 ccttcatcgg atcagccgca ggcaggtcaa ttttattcac cgcaataata atcggcacct
     8821 tcgccgcctt ggcatggtta attgcttcaa ttgtttgcgg catcacgctg tcttcggcag
     8881 aaactacaat cacgcaaata tccgtaacat tggcgccgcg tgcgcgcatt tctgtaaaag
     8941 ccgcgtgtcc aggtgtatca aggaatgtaa tcttttcacc agactccata tggacctgat
     9001 aagcaccaat atgctgggta atcccgccag cctcaccgga aacaacattg gtttgacgca
     9061 ttgcatccag cagcgaggtc tttccatggt cgacatggcc cataatggtc acaaccggtg
     9121 gtcgcgtttt aagattttcc ggcttgtcct caacaccgct taggccttct tcaacatcag
     9181 cctcggttac gcgcttgatt gtatggccga actcaccaat aaccaactcg gccgtgtccg
     9241 catcaatact ctgtgttaca gttgccatca cgccgagttt cataagtgtt ttgacaacct
     9301 ctcccgttgg ttccgtcatt ctgttagcga gttcgcccac agtaataact tcaggcacaa
     9361 ccacttcacg aaaaaccttc tgtgcaggct ccttcggccc cttcgccgcc atgcgggctt
     9421 tttcacgcgc ccggcgctgc gcagccatcg acatcccgcg gtcacggtca aagtcacgat
     9481 ttaaggcctg agttacagtc aggcggcctc cgcgtctatc ttcagcagtt ttggacggag
     9541 ctttaggcgc agccttttta agtttgtcgc gataattttc cgatgtagaa tcctcaccaa
     9601 atttcgctct tcgtgccgtg cttgggtcat cggggttgag agggatcgtc ggagcattag
     9661 cttgagcatc tgcggctttt ttggattctt cagcttctat gcgctcaagc tcttccagct
     9721 ctttttcacg cgctgatttt tcggcctgct ctttattggc agcttcttct ttcttcttgt
     9781 cttcaattgt agtgcgtcgt ttcggtaaag agcttttcgt cttgccacca tcggccagcg
     9841 ccttctcaag cgctttcgcc cgcgcctcac gttctgcgtc tgaaagatga gcatcacttt
     9901 gcggggtatt gtcctgcggt gcagaggttg gtgccgtcgt tcttttacgg cgtacttcaa
     9961 cggcaacggt tcctggtgcg atacctggtc ttggggtcgg cgcaccgccc tttaggctca
    10021 aagttccgcc aaggctcaag gttttctttc cgccttcttt cttttggtca tcactgtctt
    10081 tgtttttcgt ttcgctcata atctcttcaa ggtctttctt gtatattttg agtgtcgcgc
    10141 acacagggtt atacaccact gagccagcgg tgcaaaattt ttgtttaagt ttaaatgatt
    10201 agatggaaat gcgaaggaaa cagattccgg ctcagcgttc gttaaaacgc tggtccggtt
    10261 gataaaatat aagactgtgt atatgcggtt caaaatgttt ctgtctccca tacatcgctt
    10321 taagccgcat tctcagcggc ggcagcagat tccgcttctg ccgcgacctt gtcttccgcc
    10381 tcaaaccagt gcgcacgcgc agccataatc acggcgttgg catccttgac ggtgatttga
    10441 ccttcaccca aagtttcagc cagttcatct gccgccaaat cagccagatc atccagtgtc
    10501 ttaacacctg ttttgcctag catgataatc tgcgccggag ttaagccctc cgcgtgcatc
    10561 aaatcatcgg caattcccag ctcaacctgc ttttcagaca gctctttctg cttggcgtcc
    10621 agccatgtcg ccgcccggtt ttgcagctca gtagaaattt cttcttcaaa accctcaatc
    10681 tggtttagtt cgtcaatcgg cgtttcaaca atttcttcga ctttggtaaa gccttcggcc
    10741 accaacagat gtgcaatcac atcatcaacg tccaaagcct ccatgaatag agaagagcgc
    10801 gttttgaatt cttcagcgcg gcgctcggat tcctcggctt cagtcagaat atcaatatcc
    10861 cagccaacca aaatggaagc caggcgtaca ttctgtccac gtcgtccaat cgccaatgac
    10921 agttgctcgt caggcacaac cacttccata cgcttggttt cttcatccag aacaaccttg
    10981 gtcacttccg caggttgtaa agcatttacc acaaagccgg caatatcttc agaccacggt
    11041 acaatgtcga ttttttcacc gccgagctca ttcacaacag cctgtacgcg gctcccgcgc
    11101 atcccgacgc aagcgccaac agggtcgata gaaccttctt tggaaaccac agcgattttc
    11161 gcacgactcc ccggatcgcg ggcgacagat ttaatctcga taattccgtc gtagatttcc
    11221 ggcacttcct gcataaacag cttggccata aattcagggt gcgtacggga aaggaaaatc
    11281 tggggtccgc gcatttcctc gcgtacttca taaataatgg cacgtacacg atcgccattt
    11341 ttcaaatgct cgcgcggcaa gccttcatca cggcgtagaa ccgcttcggc gcgcccctga
    11401 atatctacag tagcattgcc gtactcaaca cgcttcacaa cgccgttaat aacttcacca
    11461 acgcggtctt taaattcgct gtattggcgt gaacgctcag cttcacgcac tttttgcatg
    11521 atcacctgct tggcggtctg cgcggcaatc ctgccaaagt ccagcggtgg caactcatca
    11581 atcaggaatt ccccaatttt ggcatccggt ttaatccgct gtgcttgaga aagcggcatc
    11641 tgcgccatct cattttcgat ttcggccgtc tcttcaacca cctcgcgata acgcttcagc
    11701 gcgatattcc cggtcttgcg gttgatctcc gcacgaatgt catgatcatg accgtatttc
    11761 gaacgcccgg ctttctggat tgcttcttcc atcgccgtga cgacgatctc gcgatcaatg
    11821 tttttctctc ttgcgacgtt atccgcaact tgtagcaatt ccatttttct ttctccttat
    11881 agatttgctg ttttttgtat taattcatca ctcatgacca atttagcctt ggtcaaattc
    11941 gagaaaggga tctcggcctt gccctgatcg gtgtcaatca atacactctc gccgtttaat
    12001 ccctgcaaaa ccccgcgaaa gcgcttttgc ccgttttccg ccgggacgca gctttcaatt
    12061 ttcgcttcaa accctttata ggtttcaaaa tcttcaatcc gcaccagcgg gcggtcgatg
    12121 cccgggctac tgacttcgag acgatacgcc cctttaatcg ggtcttcaac gtcaaacacg
    12181 gcggataaag ctcggctgat cacggccgca tcatccacgc ccagattgcg cgtcgccgga
    12241 tcttcggcca gaatctgcac gttctggctg ccgccttcac caaccacctt aatcagcaca
    12301 agcgcaaagc cgagatcttc aatcacgggc cgtgcatatt cgctcaagcg ctgttccaaa
    12361 ggtgttaact tcatcactat cctcaccgtc attgcgagga agcccgaagg gctgacgcgg
    12421 caatccagaa ttttggcttt gtggctctgg attgcttcgc tgcgctcgca atgacgcatt
    12481 aaattcaaaa aacccaacaa aaaaggcggg cctcttgatg agcccaccta tatcaaatga
    12541 tcaggttgtt gaagcatgta tacgtatgtt ttagagcata ggcaagatat ttttgcgtta
    12601 gacacccatt aatatgtccc agaacgaaaa atgataagcc cgttcaatca gatacgtttt
    12661 agatatatgg tgtttctttc cattcgccgt gaaatcgata atcacgttct cgatcatatc
    12721 gtgaggaaga tttgaatcat tataaacttg ctcataatag catatttggt ttgccccgat
    12781 tatacaggat ttaaattcct tgagattgtt tttcagtggt ggaattaaac gcccttccgg
    12841 agttctgtgc tccagagtaa tattctcaac agatgaaatt ttggattgat aggtattgaa
    12901 gataaccttt gaaaggcggg tattttttaa ggc
//
LOCUS       DAOSWR010000053        12842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3094_length_12842_cov_4.866114, whole genome shotgun sequence.
ACCESSION   DAOSWR010000053 DAOSWR010000000
VERSION     DAOSWR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_12842_cov_4.866114"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK513_05400"
     CDS             complement(<1..865)
                     /locus_tag="PK513_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HOO81917.1"
                     /translation="MPKNIYAHMQTAVDIVTTSDHPTNKIAATIAGENWALSCVNHWP
                     PVIAEKIGREADIGNSSGTIHAETACIIGAGQAGHKTDGTSIFITDPPCPNCMKNLAE
                     AGITKLYIDHKGFDKDWIRRRGDDFENMSMRIAAKAGMDVFVIYRKEERFEVISRHAP
                     GYKPSNENPIKIRHCEEEQPTKQSIEAFWIASLSLAMTQHRDEPFALALAADKDGQVF
                     SIAANCHPTIGYTSQTLEHPDNKYSYILEPVNRLLMNAPRYGLHIDPAHIYSSRVPSA
                     RELVNMIGAGLT"
     gene            942..2330
                     /gene="lpdA"
                     /locus_tag="PK513_05405"
     CDS             942..2330
                     /gene="lpdA"
                     /locus_tag="PK513_05405"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOO81918.1"
                     /translation="MAEKFDVIVIGAGPGGYVCAIRAAQLGLKVACVEKRKTLGGTCL
                     NVGCIPSKALLSASEKFEEAEHHFSDIGIDTKPKLNLKKMMGFKDDVVEANTKGIEFL
                     FKKNKVTRLEGAAEIVSAGKIKIGSDEYEADSIVIATGSDVISLPGIEIDEKKIVSST
                     GALALIEVPKNMVVIGGGVIGLELGTVWRRLGAQVTVVEFLDQILPGMDGEVRKESQK
                     IFKEQGIEFKTGMKVTSAKASATGVDLMMEPASGGAEEKMSVDIVLMAVGRKPYTDGL
                     GLDKLGVEMSERGQIKTDEFFETSVEGIYAIGDVIVGPMLAHKAEDEGVILAEMLAEQ
                     SGHIDYNLIPGVVYTWPEVANVGQTEEQLKDAGVKYNVGKFPFMANGRARAMNAMDGF
                     VKILACAETDRVLGAHMIGPNAGDLIQEVVSVMEFQGTAEDIARTCHAHPTLTEVVKE
                     AALAVHKRPIHI"
     gene            complement(2354..3106)
                     /locus_tag="PK513_05410"
     CDS             complement(2354..3106)
                     /locus_tag="PK513_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81919.1"
                     /translation="MTKHAFLLLYEIFMLYKTAYFLNNSEEIQMLTYEDLKDFQDDRG
                     GTPTPPEHKKAEALNRRINGSGNTGFQGKVVDGIIIMPSTLTILSSNIKNLKEALRCF
                     PEYDLETQIDNPEAVKVKGNLDRLKKDEVIVACTLDMRTHNPKFVSLWLKSDLVALQF
                     NEQARPLLDDSKPFSLERDTNHAIIEAPKCKTTGNKDQFYIIFSRATGEAAEKMKQRG
                     YLLTERPRIKDIPMPSGKRMAANLLCALGCDL"
     gene            3237..3596
                     /locus_tag="PK513_05415"
     CDS             3237..3596
                     /locus_tag="PK513_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81920.1"
                     /translation="MGSDVKTHYLAGDLPVVNIGAIDGLKKGFVVVSDPGSVSYTPRS
                     QRCGVPSEVVGNHVVREAHTGITLQQKFEGHAIICPCSQGDLKKGFSEGVKLGYLPLE
                     DRVHFCETPHDPIELGR"
     gene            complement(3593..4597)
                     /locus_tag="PK513_05420"
     CDS             complement(3593..4597)
                     /locus_tag="PK513_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOO81921.1"
                     /translation="MKDETLALCAADLAETLQKWQDYLRHEKQLSRHTLRAYNSDLNA
                     FLNFLSVHLNKNPGLNDLSETSIRDFRAWMSRRAMDGAGNATRARALSGIKNFLSWLD
                     KQGIMHNAAISTVRSPKLPHKIPRPLHQKQARNVLESADLLAKEDWIGQRDRALFTLL
                     YGCGLRIDEALSLNIADMPKDGFIRVIGKGNKERQVPVIEIVEKTIEAYWEICPYHQT
                     SLRAKRSNPEIDCRVEPNGSPRNDNKPLFLGTRGRRLNQGVAQKAMRTLRTTLDLPEN
                     ATPHALRHSFATHLLQNGANLREIQELLGHASLSTTQRYTEVNAEELLRIYKAAHPRA
                     "
     gene            complement(4631..5338)
                     /locus_tag="PK513_05425"
     CDS             complement(4631..5338)
                     /locus_tag="PK513_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOO81922.1"
                     /translation="MTNALTDPAEKPMITKDDLVAFLKDNPNFLSKNPDVVELLLPPK
                     KRPVRGQPADFQSYMIERLKTDKDEALQTTREIVESSRANMNNQQRIHAAILRLLEAQ
                     NFHNFIHAITMDLADLLDVDIAVLVVEADGKSVPHIQTTGITVLPEGTVDQWMGDKPV
                     LLQDNISGIEAIYGGGAALVKSQILLRVDISMDTPPAILAFGSRNPDMFNEDQATDQI
                     LFLARVIERTFRMWLSV"
     gene            complement(5392..5571)
                     /locus_tag="PK513_05430"
     CDS             complement(5392..5571)
                     /locus_tag="PK513_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81923.1"
                     /translation="MSKIPDALAPQFESMVKVAAGATAAAVLAVAVYAAFGNEKPAEQ
                     QEPTNMTTHQTEPSR"
     gene            complement(5690..6346)
                     /gene="fsa"
                     /locus_tag="PK513_05435"
     CDS             complement(5690..6346)
                     /gene="fsa"
                     /locus_tag="PK513_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOO81924.1"
                     /translation="MKFFVDTGDIDEIKALAATGMVDGVTTNPSLIAKSGKDFKEVIQ
                     AICAIVDGPVSAEVAATDYETMRAEAEVIRKWGDNIAVKVPLTEDGLRVCKELSDDGT
                     MVNVTLCFSPAQAILAAKAGASFVSPFVGRLDDISQDGLQLISDIVRIYDNYPQFDTE
                     VLVASIRNPLHIVESAKMGAHVMTAPPSVIRQLYKHPLTTSGLAAFVEDWKKTGQSIL
                     "
     gene            6484..8772
                     /locus_tag="PK513_05440"
     CDS             6484..8772
                     /locus_tag="PK513_05440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOO81925.1"
                     /translation="MQNLFQEVEEQKAQALPGAVMSVLVPYPVDKAYDYIVPEGLDLV
                     PGDYVTVPLGGREVPAVVWGEPAGDVKLGKLKAVVCKHNIKPMPGVMREFIDWVARYT
                     MSPKGFVLKMALSVPAGLNLPKPAKGYVIASSLRAERSNLEDVDCRVGSGDPPRNDVT
                     VKQQQVLDVLADGSPRIAADVVRMADVSAGVVKGLAGKGLVKEVEIFNPAPCRRPDAL
                     RSGAALSEDQDAVAGRLTEAVKAKEFHAVLLDGVTGAGKTEVYFEAVAEVLKQGRQVL
                     ILLPEIALSNAFLDRFVARFGCAPALWHSHLSGGTRKTTWRGVAEGHTKVVVGARSAL
                     FLPYQDLGLIIVDEEHESAYKQEDGVIYHARDMAVVRALLGSFPVVLVSATPSLETVH
                     NAWIGRYEHLHLPDRFGGARMPDIELVDMRDKNQKPDAQHFISAGLKQAIAETLERGE
                     QALLFLNRRGYAPLTLCRSCGHRMECPRCTAWLVEHKKTGALQCHHCGYAQRMPKACP
                     ECQEEDSFAACGPGVERIYEEVHSLFPEARILMLASDTAENSEALSKMLQDIKDDKID
                     IIIGTQIIAKGHHFPNLTLVGVVDADLGLQGGELRAAERCYQLLHQVAGRAGREDKRG
                     RVLLQTFMPEHRIMKALAEGLRDPFLETEAAEREAAHMPPYSRLVGIIVSGRDERQAF
                     EVAKALGRSAPQSSTEGGGITTLGPAPAPFARLRGKYRYRLLVQADKALDIQKTIAYW
                     VGAVKVPSTVRVYIDIDPQSFL"
     gene            complement(8776..9009)
                     /locus_tag="PK513_05445"
     CDS             complement(8776..9009)
                     /locus_tag="PK513_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81926.1"
                     /translation="MPGAISSLTSKFPALNIFTGEQDDIPEDALTESRLPENYGRTQT
                     LEKAIQHRLDQARITDEDLNPKPVHELRIAGLL"
     gene            9162..9959
                     /locus_tag="PK513_05450"
     CDS             9162..9959
                     /locus_tag="PK513_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOO81927.1"
                     /translation="MNAKALPTQITSYDLLKSFALITMIIDHIGMVFFPDDLWWRVVG
                     RMSFPVWIFLIGYARSREVPAVFWLGAVVLTLTDFITGDAVLALDILVTIGLTRLVLD
                     KVMVYLVPELSRFWLAFGVLSALVLPTFVIFDYGTMAFLIAGFGYMVRHWPKDDAAVQ
                     KLQERYMVGVILVYAGIEAAAFGFSTAQFLFVSAGVTAVFLLLLSPFKPKIHEGLDDR
                     LGPVLSGMLRLFGRHSLVIYVLHLMAFKFTAMALGLHEYGLWNWRWL"
     gene            9938..10789
                     /locus_tag="PK513_05455"
     CDS             9938..10789
                     /locus_tag="PK513_05455"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOO81928.1"
                     /translation="MELAMALSGVLKTQKISSALTSYDLLKALALVLMVVDHVGYFFF
                     PEEIWLRVIGRLSVPVWFFLIGYAKTREVPRLFWILALVVAGSTLVSGEYLFPLNILF
                     TLILARLSVDWLYRHALRNTEAFLGMFFLLSFAAPPSLVFIEYGTLGFLFTLMGALRR
                     HKGDVAAPSWMVWAFFAAGSFAYILVQSLLLPSLSAGQFFFFVAGMVVLSFLLYRFNI
                     HVFEKVNVRSFPPALLLMLLGRRTLEFYVLHLLVFRAVVMAVDPSRFAFGEFKVFAFS
                     QLLSFLL"
     gene            complement(10800..12251)
                     /locus_tag="PK513_05460"
     CDS             complement(10800..12251)
                     /locus_tag="PK513_05460"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOO81929.1"
                     /translation="MISRKGTFALFTLIILEGYVVLSSELIAIRLNLPFVGSGTDTIS
                     IIIAAILMPLAFGYHAGGRFKPGNHIIFPGFKNHINIREKLIINLLTALIFLLFALSY
                     SFIDLFFNTIYETGFKNRIIATTLYAMLFLVMPVYLLGQTIPLSCNFFKQKKLAKITG
                     KILFCSTLGSFAGAIFSTLVLMSHIGVHNTAIINFFILGLLITLLSRERMSLRIALTW
                     TLILAGVMTNSSQAMRREHSIVENNQYNTISIIQDHNERHLVLNGNMSSMYSDDHRKY
                     KYVQFIEQIALGPIWNETDNPKDILVIGAGAFTLGYGDKGNHYEYVDIDKSLKDIAEK
                     FILKQPLQNNQTFHPLPARAFLTQTNKKYDVIILDAYQGIASIPEHLITREFFAEIKD
                     HLKDDGKMLGNFAVIPNFKSAFSRNLDNTIRSVFPHVSRHSIHDYYALWQEEDSTITN
                     MIYIYSNNPDTDTKTIYSDLKNRSFLDDPGETD"
     gene            12464..>12842
                     /gene="atpH"
                     /locus_tag="PK513_05465"
     CDS             12464..>12842
                     /gene="atpH"
                     /locus_tag="PK513_05465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOO81930.1"
                     /translation="MSSSSQASSVVSQRYANALMELAEKTKKLDKVEKDLQELKTMVE
                     SSEDLRGAIRSPLYAEDSLLKAMGALADHAKFQDVTKNFLGLLVKNGRLSGLPKILEA
                     FAAALDKRRGAIAVNVNVAQDLSA"
BASE COUNT         2769 a         2526 c         3852 g         3695 t
ORIGIN      
        1 gggtgaggcc tgcgccgatc atattgacca gttcgcgcgc gctgggcacg cgggaagagt
       61 aaatatgggc cgggtcgata tgtagaccat agcgcggcgc gttcatcaac aggcggttta
      121 cgggctcaag gatatagctg tatttattgt cggggtgttc gagcgtttgt gaagtgtagc
      181 cgatggtcgg gtggcagttg gcggcaatcg aaaaaacctg tccgtctttg tcggcggcca
      241 aggcgagtgc gaagggttcg tcgcggtgct gcgtcattgc gagcgatagc gaagcaatcc
      301 agaacgcctc tatggattgc ttcgtcggtt gctcctcttc gcaatgacga attttgatag
      361 ggttttcatt gctgggttta tagcccggcg cgtggcggga gataacctca aaacgttctt
      421 ctttgcggta aattacaaac acatccatgc cggccttggc ggctatgcgc atcgacatat
      481 tttcaaaatc atcgcctcgc cgtcgtatcc agtctttgtc gaagccttta tggtctatat
      541 aaagtttggt gatcccggcc tctgctaggt ttttcatgca gttggggcag ggcgggtcgg
      601 tgatgaatat ggatgtgcca tcggtcttat gcccagcctg accggcgccg atgatgcagg
      661 cggtttcggc atggatggtg ccggaggaat tgccgatgtc tgcttcacga ccgattttct
      721 ctgcgatgac cggcggccag tgattgacgc agcttaaggc ccaattttct ccggcgatgg
      781 tggcggcgat cttgttggtc gggtgatcgc tggtggtcac gatatcgact gcggtctgca
      841 tgtgggcgta gatgtttttc ggcatggaat agaggcttgt tttttactct gtttcgcgta
      901 gtttaaaata gttttgaaca ccagaataga gaagaattcc tatggctgaa aaatttgatg
      961 tgattgtgat tggtgcgggg ccgggcggct atgtgtgtgc gatccgcgcg gcgcagctgg
     1021 gccttaaggt tgcgtgtgtg gaaaagcgta aaacgctggg cgggacctgt ttgaatgtcg
     1081 ggtgcatacc gtcgaaggca ttgctgtctg cttccgaaaa gtttgaagag gccgaacatc
     1141 atttttctga tattggcatt gatacaaagc cgaagctgaa cctcaaaaaa atgatgggtt
     1201 ttaaggatga tgtggttgag gccaatacca agggcattga atttttgttt aagaaaaaca
     1261 aggtgacgcg ccttgaaggg gcggcggaga ttgtgtcggc tggtaaaatt aaaatcggaa
     1321 gcgatgaata tgaagccgat tcaatcgtga ttgcgacagg cagcgatgtt atttcattgc
     1381 ctggtattga aattgatgag aagaaaatcg tgtcttcgac cggtgcgctg gcgcttatcg
     1441 aagtgccaaa aaatatggtt gtgattggcg gcggcgtgat cgggctggag cttggcaccg
     1501 tctggcggcg tttgggtgca caagtgaccg tggttgaatt tctcgatcaa atcctgcccg
     1561 gtatggacgg cgaggtgcgc aaagaatcgc agaagatatt caaggagcag ggcattgagt
     1621 ttaagaccgg gatgaaggtg acgagcgcca aagcgagcgc tacaggtgtg gatctgatga
     1681 tggagccagc ttccggtggt gcggaggaaa agatgagcgt cgatattgtg ctgatggcgg
     1741 tggggcgcaa accctatacg gatggtttgg gactggataa gcttggcgtg gaaatgagcg
     1801 agcgcgggca aatcaaaaca gatgaatttt ttgaaacctc tgtggagggg atctatgcga
     1861 ttggcgatgt gattgtgggg ccgatgctgg cgcacaaggc cgaggatgag ggcgttattc
     1921 tggctgagat gctggccgag caatccgggc atattgatta taatctgatc cccggcgttg
     1981 tttatacgtg gccggaagtg gcgaatgtcg ggcaaaccga agagcagctc aaagatgcag
     2041 gcgtgaaata caatgtcggg aaattcccct tcatggccaa tggccgcgcc cgtgcgatga
     2101 atgcgatgga tgggtttgtg aaaattctgg cctgcgcgga aacagatcgc gttttgggcg
     2161 ctcatatgat cggcccgaat gccggtgatc ttattcagga agtggtcagt gtgatggaat
     2221 ttcagggcac ggcggaggat atcgcgcgga cctgccatgc gcatccgacc ttgaccgaag
     2281 tggtaaagga agccgcgctg gcggtgcata agcgcccgat tcatatttga tgctctaatt
     2341 ataaggattg tttttaaaga tcacatccaa gtgcacaaag gaggtttgcc gccattcttt
     2401 tcccgcttgg cattggaatg tcctttatgc gcggtctttc tgtgagaaga tatcctcttt
     2461 gtttcatttt ttctgcggct tccccggttg cgcgggaaaa tataatatag aattggtcct
     2521 tgtttcccgt agttttgcat tttggagctt cgataattgc gtggtttgta tccctttcca
     2581 gagaaaacgg tttagaatca tccagaaggg ggcgtgcctg ttcattgaat tgcagggcaa
     2641 caaggtcgct ttttaaccag agagagacaa atttcggatt gtgcgttctc atatccaatg
     2701 tgcatgcaac aatgacctca tcttttttca ggcgatcgag atttcctttt actttgacgg
     2761 cttcaggatt atcaatttgt gtctcaagat cgtattccgg gaaacagcgg agcgcctctt
     2821 tcaagttttt aatgtttgaa gataaaattg taagtgttga aggcataatt atgatgccgt
     2881 caacaacttt gccctgaaaa ccggtgttgc cggacccgtt tatgcgacgg ttgagagcct
     2941 ctgctttttt gtgttctggt ggggtgggcg taccgcctct atcatcctga aaatctttca
     3001 gatcttcgta agttaacatt tgaatttctt cgctgttatt taaaaaatac gctgttttgt
     3061 ataacataaa aatctcatat agcaataaaa aagcatgttt tgtcactttt tgcgccttcc
     3121 tttgtggttg gttggaggtg gacaggcggg cgattcatat ttgattaaga aaaatgttgc
     3181 cttttttcat aagattatat aagaggtata tattctaatg aaaaaggaga atggaaatgg
     3241 gatctgatgt taaaacgcat tatttggcag gtgatcttcc ggttgttaat attggtgcta
     3301 tagacgggtt gaaaaaaggt tttgttgttg tctcagatcc cggcagtgtt agttatactc
     3361 cccgttctca gaggtgtgga gtgcctagcg aagttgtggg aaatcatgtt gtgcgtgaag
     3421 ctcatacagg aataacgctg caacagaaat tcgagggaca cgctattata tgtccttgtt
     3481 ctcaaggtga tcttaagaag gggttttccg aaggagtcaa attgggatac cttcccttgg
     3541 aggatagagt gcatttttgc gaaacgcctc atgatccaat tgagcttggg cgttaagccc
     3601 gtggatgggc ggctttgtag attcgcagca attcttcggc gttgacttcc gtatagcgct
     3661 gggtcgtcga caggctcgca tggcccagca attcctgaat ttcgcgtaaa ttcgcgccgt
     3721 tttgcagcag gtgtgtggca aagctgtggc gcagggcgtg gggcgtggcg ttttctggca
     3781 ggtcgagggt ggtgcgcagg gtccgcatgg ctttttgcgc gacgccttga ttgaggcgcc
     3841 gtccgcgtgt gccgagaaat aagggtttat tgtcattgcg aggagaaccg ttgggttcga
     3901 cgcggcaatc tatttctgga ttgcttcgct tcgctcgcaa tgacgtttgg tgataaggac
     3961 aaatttccca atacgcctcg atcgtttttt caacaatctc aatgaccggg acttgccgct
     4021 ctttgttgcc cttgccgatg acgcggatga aaccgtcctt tggcatgtct gcaatattca
     4081 gactcagcgc ctcatcaatg cgcaggccgc agccatagag caaggtgaag agggcgcggt
     4141 cgcgctggcc gatccagtct tctttggcca ataaatcggc ggattcaagt acattgcggg
     4201 cttgtttttg gtgtagaggg cgcgggattt tgtgtgggag tttggggcta cgcactgtgg
     4261 agatggcggc gttgtgcatg atgccttgtt tatcgagcca gctcaggaag tttttaatgc
     4321 ctgagagggc gcgggcacgg gtggcattgc ctgcgccatc catagcgcgg cgggacatcc
     4381 atgcgcggaa gtcgcggatc gatgtctcag acaggtcgtt cagtcccgga tttttgttca
     4441 aatgtacgga taagaagttt aagaatgcat tgagatcgct gttataggcc cgtagcgtat
     4501 ggcgcgagag ctgtttttca tggcgcagat aatcctgcca tttttgtagg gtttcggcca
     4561 ggtcagcggc gcagagggcg agggtttcgt ctttcatatt gaacacaggc aaaagtttaa
     4621 gacgtctggc ctagacactg agccacatcc tgaatgtgcg ttcgataacg cgggcgagga
     4681 agaggatctg gtcggtggcc tgatcttcgt tgaacatatc ggggttacgg cttccgaagg
     4741 ccagaatggc aggcggggtg tccatggata tatccacgcg cagcaggatt tgcgatttga
     4801 ccagcgccgc gccgccgcca taaatggctt cgatgccgct gatattatct tgaagcagaa
     4861 cgggtttatc gcccatccat tgatcgacgg tgccttcggg caggacagtg atgccggtgg
     4921 tttgaatgtg gggaacgctt tttccatcgg cttcaacgac cagtacggca atgtcaacat
     4981 cgagtaaatc ggccagatcc atggtgatgg cgtggatgaa attatggaag ttttgcgctt
     5041 cgagcaggcg cagaatcgcc gcatggattc gctgctggtt attcatattg gcgcgtgagc
     5101 tttcgacgat ctcgcgggtg gtttgcaggg cctcgtcctt gtcggtttta aggcgctcaa
     5161 tcatataaga ttggaaatcg gcgggctggc cgcgcacggg gcgttttttc ggcgggagca
     5221 aaagctcgac cacgtccggg tttttggaca ggaagttcgg gttgtccttt aaaaaggcaa
     5281 cgaggtcgtc tttggtgatc atgggttttt cggcagggtc ggttagagca ttagtcatgc
     5341 tcccagccta tcatagcgca tagcttaaga gaatagccgt agcgataaag tttagcggga
     5401 aggctctgtc tgatgcgttg tcatatttgt tggctcttgt tgttctgctg gtttttcatt
     5461 gccgaatgca gcgtacactg caactgctaa aactgcggca gcagttgcac ctgcggccac
     5521 tttaaccatg ctttcaaatt gtggggccag cgcgtctggt attttggaca tgtttgctat
     5581 cctttgttat gcggtccgta ggacagaaca ccacgggttt acccgtgggt atctattctt
     5641 catagtctgg ttgtaatctc aaattggcga agagtcaact tatggtaatt tacaggatgg
     5701 attgcccggt ttttttccag tcctcgacga aggcggcgag gccggaggtc gttagcgggt
     5761 gcttgtaaag ctggcgaatg accgatgggg gagcggtcat cacatgggcg cccatttttg
     5821 cgctttcgac gatgtgcaag gggttgcgaa tgcttgcgac cagcacttct gtatcgaatt
     5881 gcggataatt atcgtagata cgcacaatat cggaaatgag ttgcagtccg tcctgagaaa
     5941 tatcgtcgag gcggcctacg aacggagaga caaaagacgc gccggccttg gcggccaaaa
     6001 tcgcctgagc cggagagaag cacagcgtca cattgaccat tgtgccatca tcggaaagtt
     6061 ctttacacac gcgcaggccg tcttcggtta gcggcacttt cacggcaatg ttgtcgcccc
     6121 atttgcggat aacctcagct tcggcgcgca tggtttcgta atcggtggcg gcgacttcgg
     6181 cgctgacggg gccatcgaca atggcgcaga ttgcctgaat gacctctttg aagtctttac
     6241 ctgatttggc gataagcgat gggttggtgg tgaccccgtc aaccatgcct gttgcggcca
     6301 gagccttgat ttcatcaata tcaccggtgt cgacaaaaaa cttcatgata gtcgtttcct
     6361 cttattttta cagggtgttg ctgtgctcct aaagtagggt ttctactcgt cgtcgcttgc
     6421 gcctgctgta aaagtaagag gaaacgacta tatagcccag taaaatttgc ttataactat
     6481 cagatgcaga atctttttca agaggttgaa gagcaaaaag cgcaggcttt gccgggggca
     6541 gtgatgtctg tgctggtgcc gtatccggtg gataaggctt acgattatat tgtgccggaa
     6601 gggttggatt tggtccccgg tgattatgtg accgtgcctt tgggggggag agaggttccg
     6661 gcggtggtgt ggggtgagcc tgcgggtgat gtgaagctgg gcaagcttaa ggcggttgtg
     6721 tgcaagcata atattaagcc gatgcccggt gtgatgcgcg aatttatcga ctgggtggcg
     6781 cgttatacga tgtcgccgaa gggctttgtg ttgaagatgg cgctgtcggt tccggcgggg
     6841 ctgaatttac caaagccagc gaaggggtat gtcatagctt cgtcattgcg agcggagcga
     6901 agcaatctag aggatgtaga ttgccgcgtc ggatctggcg atcctcctcg caatgacgtt
     6961 actgttaaac aacagcaagt tctggatgtt ttggccgatg gaagcccccg gattgcggca
     7021 gatgttgtgc gcatggccga tgttagtgca ggcgttgtca aagggttggc cgggaaaggg
     7081 ctggtcaaag aggtagagat ttttaacccc gcgccgtgca ggcggcctga tgctttgcga
     7141 agtggggcgg cactgtcgga agatcaggat gcggtggccg ggcggcttac agaggctgtg
     7201 aaggcgaagg aatttcatgc ggttttgctt gatggggtga caggcgcggg gaagacggaa
     7261 gtttattttg aagcggtggc ggaggtttta aaacagggtc ggcaggttct tattttattg
     7321 ccggaaattg cgctgtcgaa tgcgtttttg gatcggttcg tggctcgttt tggctgtgcg
     7381 ccagcgctct ggcattcgca tttatcgggt gggacgcgca aaacgacgtg gcgcggcgta
     7441 gcggaaggtc atacaaaggt tgtggttggg gcgcgatcgg cgctgttttt accctatcag
     7501 gatttggggt tgattattgt cgatgaggag catgagtcag cctataagca ggaggacggc
     7561 gtgatttatc atgcgcggga tatggcggtg gtgcgggcgc ttctgggcag ttttccggtg
     7621 gtgttagttt ccgcgacgcc gagccttgag acggtgcata atgcatggat tggccggtat
     7681 gagcatttgc atttgccgga ccggtttggc ggggcgcgaa tgccggatat cgaactcgtt
     7741 gatatgcgcg ataaaaacca gaagcctgat gcacagcatt tcatttctgc cggtttgaag
     7801 caggcaattg ccgagacgct ggaacgcggg gagcaggcgt tattgttttt aaaccggagg
     7861 ggctatgcac cgctgacgct gtgccggagc tgcgggcatc ggatggagtg tccgcgttgc
     7921 acggcgtggc tggtggaaca taagaaaacc ggggcgcttc agtgtcatca ttgtggatat
     7981 gcgcagcgta tgccgaaagc ttgcccggaa tgtcaggagg aggatagttt tgcggcgtgc
     8041 gggccggggg tggagcgcat ttatgaggag gtgcacagcc tgtttcccga agcgcggatt
     8101 ttgatgctgg ccagtgatac ggcggaaaac agtgaggcgc tcagcaagat gttgcaggac
     8161 attaaggatg acaagatcga tattattatc gggacgcaga ttattgccaa ggggcatcat
     8221 ttcccgaatt tgacgttggt tggcgtggtc gatgcggatt tgggcttgca aggcggcgag
     8281 ctgcgcgcgg cggagcggtg ttatcaatta ctgcaccaag tggcagggcg cgccgggcgt
     8341 gaggataagc ggggccgcgt cttgctccag accttcatgc cggagcatag gattatgaaa
     8401 gcgctggccg aaggtttgcg tgatccgttt ttggagacgg aagcggcgga gcgagaagcg
     8461 gcgcatatgc cgccttatag ccgattggtc gggattattg tttcagggcg tgatgagcgc
     8521 caagcgttcg aggtggctaa ggcgctgggg cggtctgcgc cgcaaagctc tacagaaggt
     8581 ggggggatta cgacgctggg acctgcgcct gcgccgtttg cgcggctgcg cgggaaatat
     8641 cgctatcgtt tactggtgca ggcggacaag gcgctggata ttcaaaagac tattgcgtat
     8701 tgggtcggcg cggtcaaagt gccgtcaacc gtgcgggttt atattgacat cgatccgcag
     8761 agttttttat aggagttaaa gaagtcccgc gattctgagt tcgtgaacag gtttgggatt
     8821 gaggtcttca tcagtgatgc gggcctgatc cagtcggtgt tgaatagctt tttctaaggt
     8881 ttgtgttcgg ccgtaatttt cgggcaacct tgattctgtc aacgcgtctt ctggaatatc
     8941 gtcttgctct cctgtgaata tgtttagtgc cgggaattta gatgttagtg atgatattgc
     9001 gccgggcatg gttgtctcca aatttttgtg ttgaataatt cttttaaatc ctatggcgcg
     9061 taatatgcca aaaattcaac cttatgtcaa atattgttta cggttggggg tttgagcggg
     9121 gttgtcttcc ttttaggcgt attttatgag agcattcttt tatgaacgcc aaggccttac
     9181 caacccagat tacttcttat gatcttctga aaagctttgc cctgatcacg atgattattg
     9241 atcacattgg catggtgttt tttccggatg atttgtggtg gcgcgtggtt ggacgaatga
     9301 gctttccggt gtggattttt ctcatcggtt atgcgcgcag ccgggaggtg cctgcggttt
     9361 tctggctggg ggcggtggtg ctaacgctta cggattttat taccggcgat gcggtgttgg
     9421 cgctggatat tctggtgacg attggactga cgcgtttggt tcttgataag gtcatggttt
     9481 atctggtgcc ggaattgtct cggttttggc tggcttttgg cgttttatcg gcgttggtcc
     9541 tcccgacttt tgtgatattt gattacggga cgatggcgtt tttgattgcc gggttcgggt
     9601 atatggtacg gcattggccc aaagatgatg cggcggttca gaaattacaa gagcgttaca
     9661 tggtcggggt tattctggtt tatgccggga ttgaggcggc ggcttttggc ttttcgacgg
     9721 cgcagttttt gtttgtatcg gctggtgtta cggctgtttt cttattgctg cttagcccgt
     9781 ttaagccaaa aattcatgag gggcttgatg atcgtttggg gccggtttta agcgggatgc
     9841 ttcggttgtt tgggcgtcat tcactggtca tttatgtttt gcatcttatg gcgtttaaat
     9901 ttacggcgat ggctttggga cttcatgaat atgggttgtg gaactggcga tggctttgag
     9961 cggggtttta aaaactcaga aaatatcttc tgcgctgacg tcgtatgatc ttctgaaagc
    10021 gctggcgctg gttttaatgg tcgtggatca tgtcggttat tttttctttc ctgaagagat
    10081 ttggctgcgg gtgattgggc gtttgagtgt gccggtctgg ttttttctga tcggttatgc
    10141 gaagacgcgt gaggttccac ggctgttttg gatactggcg ctggtggtgg cgggctctac
    10201 tcttgtctcc ggggagtatc tgtttcctct caatattctt tttacactta ttttggcacg
    10261 gttgtcggtt gattggctct ataggcatgc tctacgcaac acagaagcat ttttgggcat
    10321 gttctttttg ctttcatttg cagcgccgcc gagtctggtt tttatcgaat atgggacgtt
    10381 ggggtttttg tttacactta tgggggcctt gcggcggcat aaaggcgatg tggccgcgcc
    10441 gagctggatg gtctgggcgt tttttgcagc cggtagtttt gcgtatattc ttgtgcagag
    10501 tttattgctg ccgagtttat ctgcggggca atttttcttt tttgtcgctg ggatggtcgt
    10561 tttaagcttc ctgctttatc gctttaatat tcatgtgttt gagaaggtga atgtgcgctc
    10621 ttttcctccg gcgcttttgc ttatgctttt ggggcggcgg actttagagt tttatgtttt
    10681 gcatcttttg gtatttcgcg cggttgttat ggctgttgat ccatcgcgtt ttgcttttgg
    10741 cgagtttaag gtttttgcct tttctcagtt gctttcattt ttgctatagg cctgtgatgc
    10801 taatcggtct caccgggatc atcgaggaac gatctgtttt ttaaatcgga atagattgtt
    10861 tttgtgtcgg tgtcaggatt gttgctgtaa atataaatca tgtttgttat ggtgctgtct
    10921 tcttcttgcc atagggcgta gtaatcatgg attgagtgac gtgaaacgtg tggaaatacg
    10981 ctgcgaatgg tgttgtccag attgcggctg aatgctgatt tgaagttagg tattacggca
    11041 aaatttccta acatttttcc atcgtctttt agatgatctt taatttcagc aaagaattca
    11101 cgtgtgatga gatgttcagg gatgctggca attccttgat aggcatccag aataataaca
    11161 tcgtattttt tattcgtttg tgtcaaaaat gcacgggcgg gtagaggatg gaaggtctgg
    11221 ttattttgta aaggttgttt cagaataaat ttttcggcga tatctttgag gcttttgtct
    11281 atatcaacat attcatagtg attgccttta tcaccatatc ccagggtgaa agcgcccgcg
    11341 ccgataacta aaatgtcttt cgggttgtcg gtttcgttcc aaatggggcc aagtgctatt
    11401 tgttcaataa actgaacata tttatattta cgatgatcat ctgagtacat tgaggacatg
    11461 tttccattca agaccagatg gcgttcatta tgatcttgaa taatggaaat cgtgttgtat
    11521 tggttgtttt ctacaatgga gtgttctctt ctcattgcct gtgaagaatt ggtcattact
    11581 ccggcgagta taagcgtcca ggtcagggca atgcgcaggg acatgcgttc gcggcttagg
    11641 agggtaatta ataatcccaa aatgaaaaaa ttgatgatgg cggtgttgtg gacgccaata
    11701 tggctcatga gcaccaaggt tgaaaaaatt gctccggcaa aagacccaag agtggaacaa
    11761 aataagattt tccctgtgat tttggccagt tttttctgct taaaaaaatt acagcttaaa
    11821 gggatggttt gtcctaggag gtatacgggc atgactagaa agagcatggc gtagagcgta
    11881 gtggcaatga tgcggttttt aaatcctgtt tcatatatag tgttaaaaaa cagatcaatg
    11941 aagctatagg ataaagcgaa tagtaagaat atgagcgccg tcagtaggtt gataataagt
    12001 ttttcgcgta tgtttatatg atttttaaag ccggggaata ttatatgatt tcctggttta
    12061 aaccgtccgc ctgcgtgata gccaaaggcc aagggcatca aaattgcagc aatgataatt
    12121 gaaatggtgt cggtgccgct gcctacaaaa ggcaggttta ggcgaatggc tatgagttct
    12181 gaggatagaa ctacatatcc ttcaaggata attagggtaa aaagggcgaa ggtgcctttg
    12241 cgtgaaatca tgtgttttgt atagcgttta tttgagttgg ttcaaggtgc attttttaga
    12301 atatagtttg tgtgttaaca ggatggattt tgtgcggttt ttcaggaggc aacacattgc
    12361 attgaaaacg tcctttaata aggtttttgg gcttgtcatg gtcggcgcgc tatgctaaat
    12421 cctcgcccaa cacggaaaaa gaactcaaag ggagccatag agcgtgtcat catcaagcca
    12481 agcctcttct gttgtgagtc aacgctatgc taatgcattg atggaactgg ctgaaaagac
    12541 aaagaagctg gataaagttg agaaagatct tcaggaattg aagacgatgg ttgagtcttc
    12601 tgaggatcta agaggtgcga ttcgtagtcc gctttatgcc gaggattctt tgttgaaagc
    12661 tatgggggct ttggccgatc acgctaaatt tcaggatgtt accaaaaatt ttttagggct
    12721 gttggttaag aatggccggc tcagcggttt gccaaaaata cttgaggcct ttgcggcggc
    12781 tttggataag cggcgcgggg cgattgccgt gaatgtcaat gtggcgcaag atttgagcgc
    12841 ga
//
LOCUS       DAOSWR010000054        12172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3317_length_12172_cov_6.023438, whole genome shotgun sequence.
ACCESSION   DAOSWR010000054 DAOSWR010000000
VERSION     DAOSWR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_12172_cov_6.023438"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..649
                     /locus_tag="PK513_05470"
     CDS             83..649
                     /locus_tag="PK513_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOO81931.1"
                     /translation="MNTPLPNKILKSLCVFCASSSEVHQKYIDLAADLGTMLAQARIC
                     LVYGGAQGGLMGAAANAALAAGGEVIGVIPEVLFGQERAHQGLTKLYIMRDMHERQQK
                     MAALADGFVVLPGGLGTLAEFFEILTWKQIGLYDKPIILVNIDGFWDSLIETMGNCQQ
                     QGFLHGDFLDLVRICNDLHTIRTFFVFE"
     gene            726..1769
                     /locus_tag="PK513_05475"
     CDS             726..1769
                     /locus_tag="PK513_05475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO81932.1"
                     /translation="MQYSHSKDKANDFASEALERIHSGGLSPVPQTFELWYVYYAGCD
                     PDVVRSIDVLVAKEEPITDEQCAELHQRFLSDTKENERVRQAGDQIQETIRGVKSSVS
                     EIKNVTHEYSEVLQDAAGSLGPDTTPEEVREKLQSVLDNTSNVLEHNQKLEQKLERSA
                     LVMQELQRDLEVVRQQALTDSLTNLSNRKAFDSEIVRIAEEAKADGQTFSLILMDIDH
                     FKAFNDNYGHQVGDQVLRLVAQTLIDGVKGRDVAARYGGEEFAILLPQTNLTGGIKVA
                     DSLRSVVQGKELINRNSGETLGRVTLSGGVAQYVTGEPLENLIERADSALYTAKHNGR
                     NQIAAAPAPGQKK"
     gene            1853..2710
                     /gene="dinD"
                     /locus_tag="PK513_05480"
     CDS             1853..2710
                     /gene="dinD"
                     /locus_tag="PK513_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HOO81933.1"
                     /translation="MKKDLIVALNNSFEGAAHTEDGLTYWYARDIQKLLDYSKWENFS
                     SVVIKAKEACQNSGQSIADHFADVRKMVKLGSGSEREIDDIMLTRYACYLIAQNGDPR
                     KEVVAFAQSYFAIQTRKQELLEERLEHLERLHAREKLTETEKQLSGTLYQHGVDDRGF
                     GIIRSKGDQALFGGHNTRSMKTRLGVKKGRPLADFLPTVTLKAKEFAAAITDFNVRER
                     SMYGADQIGREHVKNNKDVRGVLAKNDIQPESLPAAEDIKKVERKLKSEDQKILKKED
                     SLQKIKDAS"
     gene            2700..4925
                     /locus_tag="PK513_05485"
     CDS             2700..4925
                     /locus_tag="PK513_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO81934.1"
                     /translation="MQADSISAMAVPQPPYLEGLNDPQREAIETLDGPVLVLAGAGTG
                     KTRVLTTRLAHLLNTGRVYPSQILAVTFTNKAAQEMKERVSGLMGGQPVEGWWLGTFH
                     SLAARMLRRHADMVGLSSNFTILDPDDQVRLLKQIMEVNGVDHKKWAPKAMISFIDRW
                     KDRGLNPPQVSGDDVGDIAGGKLPLLYEIYQRRLREINACDFGDLLLHMITILKDPAN
                     KHVLDEYHNRFRYIMVDEYQDTNVAQYLWLRLLAQGHNNICCVGDDDQSIYGWRGAEV
                     GNILKFEKDFPGAQIIRLEQNYRSTNNILKAAHAVIANNDGRLGKELWSEAGEGDKIQ
                     VRGLWDGEAEARWAGEEIEQLQRKGQSLNGIAVLVRAGFQTREFEERFIKLGLPYKVL
                     GGPRFYERMEIRDALAYLRVIVQPADDLALERIINKPKRGLGDTTVQTLYAYGRSHGI
                     NLYDSIMDLTQTDELRPKVKTTLMKLLDDFGRWRSLVETMDHAELAAQVLDESGYMQM
                     WKEDKTPEAPGRVENLKELISGMQEYEALPEFLEHVALVLENQDASGGEFATIMTLHG
                     AKGLEFDCVFLPGWEEGLFPSQRSMDENGLKGLEEERRLAYVGITRAKRRAYISFAAN
                     RRMYGSWVNAIPSRFIDELPKEQTEVQSDLGLAGSGRSSHWDSSGFAPKVQKRQVASQ
                     THDRKVMSESGTVFQRGDRVFHDKFGYGKIVNIDGHKLDIAFEKSGKKRVMDSFIEKP
                     D"
     gene            4996..5949
                     /locus_tag="PK513_05490"
     CDS             4996..5949
                     /locus_tag="PK513_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOO81935.1"
                     /translation="MSMKNKIATVFGGTGFVGRQIVRELAARGVAVKVATRVPERAYF
                     LKLDGSVGQIVPVACDYNDEQSVAKAVKNSDYVVNCIGVLFERKRGDFKRAHKDIPAI
                     IAKACEEEGVQRFVHISIPNIENDASRYASTKYEGEKAVQKAFPKVSILRPSVVFGPD
                     DDFFNKFAELMRFFPVLPLIGGGKTKFQPVYVGDVADAVIAALEKPEAQGQIYELGGP
                     DIVSFKEIYEILSRYTGRKRSYISLPFWLAKVQAVFMGLMPKPLLTCDQVESLKTDYL
                     IKPSAQGLSDLGVKITGMDTILPTYLESYRSGGRFAALHGH"
     gene            5969..6925
                     /locus_tag="PK513_05495"
     CDS             5969..6925
                     /locus_tag="PK513_05495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO81936.1"
                     /translation="MRVLTLFLAMAVFVGGVVSAHCAWAARSESIAVVVNEDAITMSD
                     VNDRMALIIASSGLPNKKDVQEKLLPQIIGSLVEEQVRLQEARRLDLNVSPEDIAQGF
                     AAVAQQNNMSAEEFRSMITRGGLNIRTLEAQIRAQIAWSKVVQAKLRPQVVVSDSDVD
                     SYLERLNNNTGKPEYLVSEIFLPSVSAQEESETQQLAYKLVQEIRSGQAPFFKVAQQF
                     SQSAGAPQGGDLGWIAQGQLQQELDEVLARIPQGQVSDPIRSASGYHILNVRDQRLIA
                     VENLPGREQVMGVIGMQRLERLQRRYLMDLKSAAFIENRLAQ"
     gene            6927..7754
                     /gene="rsmA"
                     /locus_tag="PK513_05500"
     CDS             6927..7754
                     /gene="rsmA"
                     /locus_tag="PK513_05500"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOO81937.1"
                     /translation="MPVFELEALPPLREVIAQHGLRAEKALGQNFLFDQNITNKIVRQ
                     AGDLCGKTVIEIGPGPGGLTRSLLLSDAKKVIALEFDLRAVAALSGLVEAAQGRLQVV
                     HADALATDLTALCAGPRVIVANLPYNIATPLLTGWLAQIRGDSGVYDEMILMFQKEVA
                     QRIVAPVGGKSYGRLSVISQWLCEVRRCFDLPPSAFTPPPKVKSSVVRFVPKALAADA
                     PEFSAVEAVSAAAFGQRRKMIRSSLGDYADAVAALGLDPTGRAETLSPVDFIQLAKY"
     gene            7808..8257
                     /locus_tag="PK513_05505"
     CDS             7808..8257
                     /locus_tag="PK513_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81938.1"
                     /translation="MSEITKYFYEGTYPVTKDSKNRVAMPKPLHDVFLQARDSDDYIS
                     SYKPGTNRILFLPRKLLTDLFNTQDLREISPHIRPFSTKSLGRFVLPKDQVLFLDISD
                     SRELIMTGNHDYWSLYKQEVWMRHFENITNHPSNPGQSPKGLEPGDV"
     gene            complement(8196..8810)
                     /gene="gmk"
                     /locus_tag="PK513_05510"
     CDS             complement(8196..8810)
                     /gene="gmk"
                     /locus_tag="PK513_05510"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO81939.1"
                     /translation="MYVLSSPSGAGKTTITRALLKNNENLTPSISATTRPRRAGEVHN
                     QDYIFVSQDEFRDMIDNGQMLEHAKVFDNYYGTPRAPVEKALASGQDVIFDIDWQGTQ
                     QLFELAAEDLVRVFILPPSNQALEKRLRDRSRDTKETEDQIRSRMAKAADEMSHYTEY
                     DYVIINTDVEKAITQAQLILDAERLKRRRVQGLSDFVRGLMGGL"
     gene            9268..9528
                     /locus_tag="PK513_05515"
     CDS             9268..9528
                     /locus_tag="PK513_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO81940.1"
                     /translation="MLKALSNERRLLIVCILHMGEKSVGALEEVIGLSQSALSQHLAR
                     LRRDGIVKTRRDAQTIYYSLNSGEAEQILACLHDIYCDEDAC"
     gene            complement(9542..10507)
                     /locus_tag="PK513_05520"
     CDS             complement(9542..10507)
                     /locus_tag="PK513_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOO81941.1"
                     /translation="MPDHTNKQKILVIKLSALGDFIQALGPMAAIREHHKDAHITILT
                     TRAFEGFARKCGYFDEVWLDTRPKLLNFKGWATLRKRLNDGKFTRVYDLQNNDRTNFY
                     FKLFKNKPEWIGAAPGASHRNTSPKRTAGHAFDGHVQTLALAEISDIKIDRLDWMNGD
                     LSNFTLKDPYVLLVPGCAPQHPQKRWPAENYARLAQQLVKMNYQPVLIGTESDAAATT
                     TIANTCPEALNLTNQTTLEQIATLGRNAAATIGNDTGPMHLIAATACPCLVLFSVTSN
                     PTRHAPKGENVKVLQKNNLQDLDIETLLQHLTMRESPKSAAKSSH"
     gene            complement(10508..11722)
                     /locus_tag="PK513_05525"
     CDS             complement(10508..11722)
                     /locus_tag="PK513_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO81942.1"
                     /translation="MNAFQIQPVIMQIIPELGPGGAEQGCIDIAAELVASGAQAIIVS
                     NGGSRVHELERIGAVHINLPVHSKSPLVMWHNITALRKIIERYNVNIVHVRSRAPAWS
                     AYYACKKTSAHYITTCHAPYNISGEAKKFYNSSIAQGERVIAISNYVADYLRKNYNLD
                     NRTIRLIPRGIPMERFHPTAVTPQRMIALAQSWRVPDGANIVMLPGRITRWKGHSVLI
                     NALERIQREDLFCVIIGSDQGRKEYRKELDEQIAAKGLEGRVRIVDHCNDMPAAYMLS
                     TVVVCPSTDPEGFGRVPVEAQAMGRPVVASDHGGAQETILRGQTGWLVEPSNPQALAA
                     AIEDALALTPTQRSMLATRAMTHIAANFTKEQMADKTLDVYAELLRGEIAPSSGEYPF
                     HSQQKSYAQTGL"
     gene            11864..>12172
                     /locus_tag="PK513_05530"
     CDS             11864..>12172
                     /locus_tag="PK513_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO81943.1"
                     /translation="MTNLSLQSASEPLSTLKREGKSDLAYVYIPPGPAGEALPLVMFC
                     GGYRSDMNGTKALYLEERCKARGQGYVRFDYSGHGASGGEFNEGTIGAWLGDAEDVLD
                     H"
BASE COUNT         2926 a         2345 c         3531 g         3370 t
ORIGIN      
        1 gatgtgtctg cggcgtgtat aaatcgtctg acaattacag ccgaggcttg tgggcaaagc
       61 catacgttgt attggaccgg gaatgaatac gcctttgccg aataaaattt taaaatcttt
      121 atgcgtgttt tgcgcatctt catctgaggt tcatcagaaa tatatagatt tggccgcaga
      181 tttgggaaca atgttggcgc aggcgcgtat ttgtcttgtt tatggcgggg cacagggcgg
      241 gttaatggga gcggcggcta atgcggctct tgcggctggt ggtgaggtga ttggggtaat
      301 tcctgaagtg cttttcgggc aggagcgtgc acatcaagga ctaacgaagc tctatataat
      361 gcgcgatatg catgagcgtc agcagaaaat ggcggcgttg gctgacgggt ttgtggtctt
      421 gccgggcgga cttggtactt tagcggaatt ctttgagatt ttgacgtgga aacagattgg
      481 tttgtatgat aagccgatca tattggttaa tattgatgga ttttgggatt ctttgataga
      541 aaccatgggt aattgccagc agcagggttt tttgcatggt gattttctgg atttggttcg
      601 aatttgcaat gatttacaca caataaggac tttttttgta tttgagtaat atattttact
      661 tttgttgaat gagttggctt gttagagttt gtttggatgc attattgagt agaaattaat
      721 acaccgtgca atattcacat tcaaaagata aggcaaatga ctttgcgagc gaagctctgg
      781 agcgtattca ctccgggggt ttgtcacctg tgccgcaaac ttttgagctt tggtatgttt
      841 actatgccgg atgtgatcca gatgttgtgc gctcgattga tgtgcttgtg gcgaaggaag
      901 aacctattac cgatgagcaa tgcgcggagt tgcatcagcg atttttgagc gatactaaag
      961 aaaatgagag ggtgcgtcag gctggtgacc aaattcagga gactatcagg ggcgttaaaa
     1021 gcagtgttag cgaaattaag aacgttacac atgaatatag tgaggttttg caggatgctg
     1081 cgggttcgtt aggtcctgat accacgccgg aggaagttcg cgagaaactt cagtctgtac
     1141 ttgataatac gtcgaacgtt cttgagcata accaaaagct agagcaaaag ctggaacgta
     1201 gcgcgctggt gatgcaggaa ttgcagcgtg atctggaagt tgtacgccag caagctttga
     1261 ccgatagcct aaccaatttg tcaaaccgta aggcttttga tagcgaaatt gtccggattg
     1321 ctgaagaagc taaggctgat ggacagacgt tttcattgat tctgatggat attgatcatt
     1381 ttaaagcatt caacgataat tatggacacc aggttgggga tcaggttttg cggcttgttg
     1441 cacagacatt gattgatggc gttaaagggc gcgatgttgc agcccgttat ggtggagaag
     1501 agtttgcaat tcttttaccg caaacgaatt tgacaggcgg aataaaggtt gccgattctc
     1561 tgcgaagcgt tgtgcagggt aaggaattga ttaaccgtaa ttccggtgag acattggggc
     1621 gggttacttt gtccggcggt gtggcacaat atgttactgg cgagccattg gagaacctga
     1681 ttgagcgtgc agattcagcg ctttatacgg ccaagcataa tgggcgcaac caaattgccg
     1741 ccgcgcctgc accggggcag aagaaataag ttagaagatt tgtaattgct tgcatcttta
     1801 tgatggaatc ggcaataatc tttgttgaga acaaatcaga aacggaattg ttatgaagaa
     1861 agatttgatt gttgctctta acaatagttt tgagggcgct gcgcatactg aagatggttt
     1921 gacatattgg tatgcgaggg atattcagaa actactggat tattcgaagt gggaaaattt
     1981 ttcatcggtt gttattaagg caaaagaagc ttgtcaaaat agtggacagt ctattgcgga
     2041 ccattttgcc gacgtcagga aaatggttaa gcttggctct ggttctgaaa gagagattga
     2101 tgacataatg ttaacacgtt atgcgtgtta tctgatcgca cagaatggtg atccccgaaa
     2161 agaggttgtt gcgtttgcac aaagctattt tgcaattcaa accagaaaac aggagctttt
     2221 agaagaacgg cttgagcatc ttgaacggct tcatgcccgt gaaaagctta ctgaaaccga
     2281 aaaacagtta tccgggacgt tgtatcagca tggtgtggat gacaggggtt ttggaattat
     2341 tcgttctaaa ggagatcagg cgctttttgg tgggcacaat acgcggagta tgaaaacacg
     2401 tcttggcgtt aaaaaagggc gtccgttggc agatttttta ccgacagtga cactcaaggc
     2461 gaaagagttt gctgcggcga ttacggattt taacgttcgt gagcgttcta tgtatggagc
     2521 tgatcaaatt ggtcgtgagc atgttaagaa taacaaagat gttcgcgggg ttttggcaaa
     2581 gaatgatatt caacctgaaa gccttcctgc tgcagaagat ataaagaagg ttgagagaaa
     2641 gcttaaatct gaagatcaga aaattttgaa aaaagaagat agtttgcaga agattaaaga
     2701 tgcaagctga ttcaatttcc gctatggctg tgccgcaacc cccatattta gaaggtctta
     2761 atgatccgca acgcgaagcg attgagacgc tggatggacc tgttttggtg ctggctggtg
     2821 cagggacggg caagacgcgg gtgctgacca cgcgtttggc gcatttgctt aatacgggcc
     2881 gggtgtatcc atcgcaaatt ctggcagtaa ccttcactaa caaggccgcg caggaaatga
     2941 aagaacgcgt aagcggcctt atgggcggac aacccgttga aggttggtgg ctgggcacgt
     3001 ttcactcgct ggcagcgcgg atgcttcggc ggcatgcgga tatggtgggt ttatcaagca
     3061 attttacgat ccttgatccc gatgatcagg tgcgtctttt aaagcagatt atggaagtca
     3121 atggggtcga tcataagaaa tgggcgccca aggctatgat cagttttatt gatcgctgga
     3181 aggaccgggg gcttaaccca cctcaggtaa gcggtgatga tgttggcgat attgccgggg
     3241 gcaagttgcc gttgctgtat gagatttacc agcggcgctt gcgggagatt aatgcgtgtg
     3301 attttggtga tttattgctc catatgatta cgatcttgaa agacccggcc aataagcatg
     3361 tgctcgacga gtatcacaat cgtttccgct atatcatggt ggatgaatat caggatacca
     3421 atgtggcgca atatttgtgg ctgcggttgc ttgcacaggg tcataacaat atttgctgcg
     3481 tgggggatga tgatcaatcg atctatggct ggcgcggggc ggaagtcggt aatattttga
     3541 agtttgaaaa agattttcct ggtgcgcaaa ttatccggct ggaacaaaac tatcgctcga
     3601 ccaacaatat tttaaaggct gctcatgcag tgattgccaa taatgacggg cggcttggaa
     3661 aagagctgtg gtcggaagcc ggggaaggcg acaagattca ggtacgaggc ctgtgggacg
     3721 gtgaggccga ggctcgctgg gccggtgaag aaattgagca gcttcaacgt aaggggcaat
     3781 cactcaatgg tattgctgtt cttgtgcgcg cgggctttca gacgcgcgaa tttgaggagc
     3841 ggtttatcaa gcttggtttg ccgtacaaag ttttaggcgg gccacgattt tatgagcgga
     3901 tggagattcg cgatgcgctg gcttatttgc gggtgattgt gcaaccagcg gatgatctgg
     3961 cgttggagcg gattatcaat aaacccaagc gtggacttgg tgatacaact gtgcaaacgc
     4021 tgtacgcgta cgggcgcagt catggaatta acctgtatga ttcgattatg gacctgacgc
     4081 aaaccgatga actccggcct aaggttaaaa cgacgctgat gaaattgctg gatgatttcg
     4141 ggcgttggcg ctcgctggtt gagacaatgg atcatgcgga gctggcggcg caggttttgg
     4201 atgagtcggg ctatatgcag atgtggaaag aggacaagac gcctgaagca cctggcaggg
     4261 tggaaaattt gaaagagctg atttccggga tgcaggaata tgaggccttg ccggaatttt
     4321 tggagcatgt ggcgctggtg ctggaaaatc aggatgcttc gggcggcgaa tttgcgacga
     4381 ttatgacgtt gcacggggcg aaggggctgg agtttgattg cgtatttttg cccggctggg
     4441 aagagggctt gtttccatcc cagcgctcta tggatgaaaa tgggttgaag gggctggagg
     4501 aagagcggcg tttggcctat gtcgggatca cgcgggcgaa gagacgcgct tatatttctt
     4561 ttgccgccaa ccggcggatg tatggaagct gggtgaacgc gatcccatcg cgctttattg
     4621 atgagttgcc gaaagagcag acggaggtgc agtccgatct ggggctggcc ggtagcgggc
     4681 ggtcatcgca ttgggatagt tcggggtttg caccaaaagt tcagaagcgg caagttgctt
     4741 cacagactca tgatcgcaaa gtgatgagcg aaagcggtac agtttttcaa cgcggggatc
     4801 gggtgtttca tgataaattc ggttacggca aaatcgtcaa tatcgacggt cataagctcg
     4861 atattgcgtt tgaaaaatca ggcaagaagc gcgtgatgga tagttttatt gagaagcctg
     4921 attaaaacct gcaggcttgg ctggatattt tgtttgaaac ccgccatatt ggaggggttg
     4981 ttttggaggc ttattatgag catgaagaat aaaattgcga ctgtgtttgg cgggacgggg
     5041 tttgtcgggc ggcagatcgt gcgagagctt gcggcgcgcg gcgtagcggt taaggttgct
     5101 acgcgggtgc cggagcgggc ctattttttg aagcttgacg gcagtgttgg ccagattgtt
     5161 ccggttgcct gcgattataa tgatgagcaa agcgtagcca aggcagtaaa gaattctgat
     5221 tatgtagtga attgcatcgg cgttttgttt gagcgcaagc gtggggattt caagcgggcg
     5281 cataaagata ttccggcgat cattgccaaa gcttgcgagg aagagggtgt gcagcggttt
     5341 gtgcatattt ctattccgaa tatagaaaat gatgcatcac gctatgccag cacaaaatat
     5401 gaaggtgaaa aggcggtcca aaaggcgttt cccaaagtat caattttacg gccgagcgtt
     5461 gtgtttggtc cggatgatga tttcttcaat aaatttgctg aattgatgcg gttttttcct
     5521 gttttgccgt tgattggcgg gggaaaaacc aagtttcagc cggtctatgt cggggatgtt
     5581 gccgatgcgg tcatagccgc attggaaaag ccagaggcgc aaggtcagat ttatgagctg
     5641 ggcgggccgg atattgtgag ttttaaagag atttatgaga ttctttctcg ctataccggg
     5701 cgcaaaaggt cttatatctc tttgccgttt tggttggcca aggtgcaagc ggtttttatg
     5761 gggcttatgc ccaagccgtt gctgacctgt gatcaggtgg aatctctgaa aacggattat
     5821 ttgattaagc cttctgcgca gggtttgtct gatttgggcg taaaaatcac agggatggat
     5881 acgattttac caacctatct tgagagctat cgttccggtg ggcgttttgc tgcgcttcat
     5941 gggcattaaa ttataaggat ttcacaaaat gcgtgttttg actttatttt tagcgatggc
     6001 ggtttttgtt ggcggagttg tttccgcgca ctgcgcatgg gcggcgcgtt ctgagagcat
     6061 tgccgtggtg gtgaatgaag atgctatcac gatgtcggat gtgaatgatc ggatggcatt
     6121 gattatcgcg tcttcaggtt tgccaaataa aaaagatgtt caggaaaaat tgctgccgca
     6181 aattataggt tcgttggtag aggaacaggt gcgtttgcag gaagcgcggc ggctcgattt
     6241 gaatgttagt ccggaggata ttgcgcaagg gtttgccgct gtcgcgcagc agaataatat
     6301 gtcggcggaa gagtttcgca gcatgattac acgcggcggc ttgaatatcc gtacgctgga
     6361 ggcgcagatt cgtgcacaga tagcctggag caaggttgta caggcaaagt tgcggccaca
     6421 ggtcgttgtt tcggatagtg atgttgatag ttacttagag cgtttgaaca acaatacggg
     6481 caagccagag tatcttgtgt ccgaaatttt cctgccgtct gtcagtgcgc aagaggagag
     6541 tgagacgcag caattggcgt ataaactggt gcaggaaatt cgcagcggtc aggcaccatt
     6601 ttttaaggtt gcgcagcaat tttcacaatc agccggagca ccgcaaggtg gagatttggg
     6661 ctggattgcg caagggcaat tgcagcaaga gctggatgag gttttggcgc gcattcccca
     6721 ggggcaagtg agtgatccaa ttcgtagcgc ttcggggtat cacatcctta atgtgcgcga
     6781 ccagcgttta atcgcagttg aaaatctgcc gggccgtgag caggtgatgg gtgtgattgg
     6841 aatgcagcgt ttagagcgtt tgcagcgtcg ctatttgatg gatttgaaat ctgcagcctt
     6901 cattgagaat cgtcttgccc agtgatttgc cggtttttga attagaggct ttaccgcctt
     6961 tgcgtgaagt gatcgcgcag catggtttgc gggcggaaaa ggcgttggga caaaattttc
     7021 tgttcgatca aaatattacc aataaaatcg tgcgtcaggc cggggatttg tgcggtaaaa
     7081 ctgtgattga gattgggccg gggccgggcg gtttgacgcg ctctttgttg ctcagcgatg
     7141 cgaagaaggt gattgcgctg gaatttgatc tgcgtgcggt ggcagcgttg agtgggcttg
     7201 tcgaggccgc gcaagggcgg ttacaggtgg tgcatgcgga tgctctggct acggacttaa
     7261 cggcgttgtg cgccggccct cgtgtcattg tcgccaattt gccgtataat attgccacgc
     7321 ctttgcttac tggctggctc gcgcaaatcc gaggcgattc cggcgtttat gatgaaatga
     7381 tcctgatgtt tcaaaaagaa gtggcgcagc gcattgttgc gccggttggg ggaaaatctt
     7441 acgggcgttt gtctgtgatt agccaatggc tgtgcgaagt gcggagatgt tttgatttgc
     7501 cgccgagcgc ttttacgcca ccaccgaagg tgaaatcgag cgtggtgcgt tttgttccca
     7561 aagcgttagc ggcggatgcg ccggaatttt cggctgtgga agcggtgagc gccgcagcgt
     7621 ttggtcagcg gcgcaaaatg atccgttcca gtcttggcga ttatgcggat gcggttgctg
     7681 cgttggggct tgatccgact gggcgcgcgg aaaccttgag tccggtggac ttcatccaac
     7741 ttgccaaata ttgacataat atacgccttg tggtacttcc tccaaaggtt gtatgggagt
     7801 aagaattatg tctgagataa caaaatattt ttatgaaggc acatatccag taacaaaaga
     7861 ctcgaagaat cgggtagcta tgccaaaacc cctacacgat gttttcttac aagcgcgtga
     7921 ttccgatgat tatatttcct catataaacc aggaaccaat agaatccttt tccttccccg
     7981 aaaactttta acagacttgt ttaatacaca agatctaaga gaaatatcac ctcacataag
     8041 acctttttct actaagtcac taggacgttt tgtattaccg aaagatcagg ttttgttcct
     8101 agatatttct gacagtagag aattaattat gacggggaat catgattatt ggtctcttta
     8161 taaacaagag gtatggatgc gtcattttga aaacattaca aaccacccat caaaccccgg
     8221 acaaagtccg aaaggccttg aacccggcga cgtttgaggc gctcggcatc cagaataagc
     8281 tgggcctggg tgattgcttt ttcgacatcg gtattgataa ttacgtaatc atattcggta
     8341 tagtgcgaca tttcatcggc ggccttggcc atgcggctgc gaatttgatc ttcggtctcc
     8401 tttgtatcgc gggaacgatc acgcaggcgt ttttccagag cctgattgga aggaggcaga
     8461 ataaaaaccc ggacaagatc ttcggcggcc agttcaaaga gttgctgcgt cccctgccag
     8521 tcaatatcga agattacgtc ctgaccgctt gctagcgctt tttcaacagg agcgcgcggt
     8581 gtaccataat aattatcaaa aactttggca tgttcgagca tttgcccatt gtcgatcata
     8641 tcgcggaatt cgtcctgaga gacaaatatg tagtcctggt tatggacttc cccggcgcgg
     8701 cgcggtcttg tggttgccga aatagaagga gtcaggtttt cattgttttt caatagcgcg
     8761 cgcgtgatgg ttgttttgcc agcccctgac ggggaagaca gaacatacat aagaccgcgg
     8821 cgtttaagag tttccaagtt tatgaacctt gtgtttttga ggtggcgttc gtgtttctcc
     8881 ggtcaaattt aagcatcctg acgcaggcgg cgcaatgcaa aattgcctgc aattgtgctg
     8941 agtaaaattt gttttaatgt tttgatctgt gataccaata gttcgttcat ggggtttttt
     9001 agtatgattt ttgagtcagg gaagagtttt ggtaagaatt tcagaaattt aaataattgg
     9061 aaaatatttt aacgtgattg aattttatta gatatgagtg ttaaatgatt atgggcttaa
     9121 gatctgcata ttgagttttc cttttgtcaa actgatctta atctgcgtga tttctggttg
     9181 ttttgttagc attttgaact caggaatagg ataaaagtgc ctagggatat aaaaaaagtc
     9241 gaaaaaaatg ttgaagtcgc agctaagatg ttgaaagcct tatcgaatga gcgacgtttg
     9301 ttaatcgtgt gcattttaca catgggagag aagagtgttg gcgcgctgga ggaagtgatt
     9361 ggtcttagtc agtccgcgct ttctcagcac cttgcacggt tacgcaggga cggtattgta
     9421 aaaacccgtc gggatgcgca gacaatttat tactccttga attctggtga agcggaacaa
     9481 attcttgcct gtttgcacga tatctactgc gatgaggatg cctgctgatt cgtttttgtg
     9541 tttaatgtga tgatttcgcg gcgctttttg ggctctctcg cattgtcagg tgttgtagca
     9601 gcgtctcaat atctaaatcc tgtagattgt tcttttgcaa cactttgacg ttctcgcctt
     9661 ttggggcgtg gcgggttggg ttgctggtta cggaaaacaa gacaaggcat ggacaggcgg
     9721 tggcagcgat caggtgcatc gggcctgtgt cgttaccgat ggtggcggct gcgtttcgtc
     9781 ctagcgtggc aatttgttct aaagttgttt gattggttaa attcaatgct tcggggcagg
     9841 tgtttgcgat ggttgttgtc gctgcggcat cggattcggt gccgatgagt acaggttgat
     9901 agttcatttt tacaagttgt tgtgcgagcc gggcataatt ttcggcgggc cagcgttttt
     9961 gcgggtgctg cggtgcgcaa ccaggtacaa gtaatacgta cgggtctttg agggtgaagt
    10021 tggagagatc gccgttcatc caatcaagac gatcgatttt aatatcactt atttctgcaa
    10081 gtgctaatgt ttgtacgtgt ccgtcgaacg catgcccggc tgtacgtttc ggcgatgtat
    10141 tgcggtgaga ggctccgggc gcggcgccga tccattctgg cttatttttg aagagtttga
    10201 agtaaaagtt ggtgcgatcg ttgttttgca ggtcataaac acgggtaaat ttgccatcgt
    10261 ttaaacgttt gcgcagggta gcccagcctt tgaaatttaa aagttttggc cgcgtgtcga
    10321 gccatacttc gtcaaaatag ccgcatttcc ttgcaaaccc ttcaaaggcc cgcgtggtca
    10381 gaattgtgat atgtgcgtct ttatggtgct cacggattgc cgccataggg ccgagtgcct
    10441 ggatgaaatc acccagcgcg ctgagtttga tgaccaggat tttttgcttg ttggtgtgat
    10501 ccggcattta aagtcctgtc tgggcatagc ttttttgttg gctatgaaag ggatattcgc
    10561 cgctggacgg ggcgatttca ccgcgcaaga gttcagcgta tacatcaaga gttttgtcgg
    10621 ccatttgctc ttttgtaaaa ttggcggcga tgtgagtcat ggctcgcgtg gcaagcatag
    10681 agcgctgtgt cggggtgagg gccaatgcat cttcgatggc ggctgccagc gcttgcggat
    10741 tagagggttc gaccagccaa ccggtttgac cgcgtaaaat tgtttcttgg gcgccgccgt
    10801 ggtcagaggc aacgacaggg cggcccatgg cctgggcttc gacgggaacg cggccaaaac
    10861 cttctggatc tgtggatggg cagacaacaa ccgttgagag catataggcg gcgggcatgt
    10921 cgttgcagtg atcgacgatt cgcacgcggc cttccagtcc cttggcggcg atttgttcat
    10981 caagttcctt gcgatactct ttacgtccct gatccgagcc gataataacg cagaacagat
    11041 cctctctttg aatgcgttct agtgcattta tgagaacaga atgacctttc cagcgtgtga
    11101 tacgtcccgg gagcattacg atattagcgc cgtcaggaac gcgccagctt tgcgcgaggg
    11161 cgatcatgcg ctggggcgtt acagcggtgg gatgaaagcg ttccatcgga atgccacgcg
    11221 ggatcaggcg aattgtacga ttatccaaat tataattttt gcgcagataa tcggcgacat
    11281 agtttgaaat cgcgatgacg cgctctcctt gcgcgattga gctgttatag aattttttgg
    11341 cttcaccgct gatattatag ggcgcgtggc atgtggttat gtaatgagcc gatgtttttt
    11401 tgcaggcata ataagcgctc cacgccgggg cgcggctgcg aacatgaacg atgttgacat
    11461 tataccgctc gataattttg cgcagggcgg tgatgttgtg ccacatgacg agcgggcttt
    11521 tggaatgaac aggcaggttg atgtgcaccg caccgatgcg ttcaagttcg tggacccggc
    11581 tgccgccatt tgatactata atggcctgtg cgccgcttgc aaccaattca gcagcaatat
    11641 caatacaccc ttgctccgcg ccaccggggc cgagttcagg gataatctgc ataatgacag
    11701 gttggatttg gaatgcgttc ataaacagcc tttgcggaat attaactcag ggggtgcagc
    11761 tacacacaaa acttgcgtac tggccacatt tgcctagatc gtttatctat ataggcagga
    11821 catcattttc gcaagatgat catcaataag gataagagaa tttatgacaa atttatcgct
    11881 gcaaagtgct tctgagcctc tctctacgtt gaaacgcgaa ggtaagtctg acttggctta
    11941 tgtgtatatt ccgccgggcc cagccgggga ggcgttgccg ctggtcatgt tttgcggagg
    12001 atatcgttca gatatgaatg gaaccaaggc cttatatctg gaagaacgct gtaaagcacg
    12061 ggggcagggc tatgtgcgtt ttgattatag cggccatggc gcttcgggtg gtgaatttaa
    12121 tgagggtacg attggcgcct ggttaggcga tgcagaggat gttctggatc at
//
LOCUS       DAOSWR010000055        12142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3328_length_12142_cov_5.990072, whole genome shotgun sequence.
ACCESSION   DAOSWR010000055 DAOSWR010000000
VERSION     DAOSWR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_12142_cov_5.990072"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PK513_05535"
     CDS             complement(<1..896)
                     /locus_tag="PK513_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO81944.1"
                     /translation="MTREHAIENYRNFGIMAHIDAGKTTTTERILFYSGKSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTTFWDAPKDGPFSGQRFRLNIIDTPGHVDFTIEV
                     ERSLRVLDGAIAVFDANAGVEPQTETVWRQADKYHVPRMCFVNKMDKIGADFYNTVDM
                     IIDRLGAVPCVIQLPIGAENNYVGHVDLVRMKAVVWDGEALGATYAYEDIPADLADKA
                     AEYREKLVELAVEQDDAAMEAYLEGNEPDEETLKKCIRKGTIAGAFVPVMCGTAFKNK
                     GVQPMLDAVVEYLPSPLDIGAV"
     gene            complement(933..1466)
                     /gene="rpsG"
                     /locus_tag="PK513_05540"
     CDS             complement(933..1466)
                     /gene="rpsG"
                     /locus_tag="PK513_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO81945.1"
                     /translation="MSRRRRAEKREILPDPKFADLVLSKFINNLMVDGKKSIAEGIVY
                     KAIDILETKAGNDNKSSDEGNSGEGDVAVAMKSKGLALFHNALKNVKPKLEVRSRRVG
                     GATYQVPVEVRHDRAMALAMRWIIDAARKRSENTMVDRVASELLDAANERGNAVKKRD
                     DTHKMAEANKAFAHYRW"
     gene            complement(1480..1674)
                     /locus_tag="PK513_05545"
     CDS             complement(1480..1674)
                     /locus_tag="PK513_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81946.1"
                     /translation="MSEYKHFEQGMDIVAQHTAAVALGGLNAEQRLELDSVYAPRHEN
                     YTAAVREYLEIYGPDICGIK"
     gene            complement(1690..2061)
                     /gene="rpsL"
                     /locus_tag="PK513_05550"
     CDS             complement(1690..2061)
                     /gene="rpsL"
                     /locus_tag="PK513_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOO81947.1"
                     /translation="MPTIQQLVRKPRQKVVKAKKNRALKSNPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTTGLEVICYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYTIIRGTL
                     DTQGVKDRKQARSRYGAKRPK"
     gene            complement(2291..2875)
                     /locus_tag="PK513_05555"
     CDS             complement(2291..2875)
                     /locus_tag="PK513_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81948.1"
                     /translation="MSILNEKTLKEISADPEQLSIDIPDFASLVEHMGFSFFEGIAQQ
                     SGAENNFVLPVLSKIKALVRTAQEQEKKLPQALYLGLEVTVKPYIFEVLPNGAGQVET
                     ETHSFNIPRISAEFTSAAAGFMCFKSYDAAKDFIEAVDPQGSAIAPLASHRCLDAWKI
                     GALYEVENMHVTRPKTSNTWPVDLSKNTEPDQPR"
     gene            complement(3002..7228)
                     /gene="rpoC"
                     /locus_tag="PK513_05560"
     CDS             complement(3002..7228)
                     /gene="rpoC"
                     /locus_tag="PK513_05560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO81949.1"
                     /translation="MNELMNLFGQPTGPQSFDSIRISIASPEQIRSWSFGEVKKPETI
                     NYRTFKPEKDGLFCARIFGPIKDYECLCGKYKRMKYKGIICEKCGVEVTLTKVRRERM
                     GHIDLASPVAHIWFLKSLPSRIGLIMDMTLKDLERVLYFENFIVVEPGMTPLKQYQLL
                     NEEEYYAAQDEYGEDNFRAGIGAEAMKEILSSLDLEEIKVQVEEDLRETGSEAKRKKL
                     VKRLKLLDSFLESGARPEWMILDIIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLIELRAPDIIVRNEKRMLQESVDALFDNGRRGRVITGANKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGIPKKMALELFKPFIYHKLELYGMA
                     STVKAAKKMVEKERPEVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAINL
                     HPLVCTAFNADFDGDQMAVHVPLSIEAQLEARCLMMATNNILSPANGKPVIVPTQDII
                     LGLYYLSLARDGEPGEGMIFRGAGEITHALEQKVLSLHAKIKCRYHTVDENGNPKTEI
                     VESTPGRILISDLLPRHPELPFSMINQVLTKKELSCVIEESYRHCGQKDTVIFADRLM
                     KLGFRYACIAGISFGKDDLIIPDEKQKLVGEANDKVKEFEQQYQDGLITKGEKYNKVV
                     DIWSKCTDDVADAMMSKISNIESGNLNAVYMMAHSGARGSAAQIRQLAGMRGLMAKPS
                     GEIIETPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVAQDAIV
                     TEEDCGTEKGISMRAVMNGADVVVSLSERIMGRCASIDVINPLDGKTIVKAGEMFDEN
                     NTEAIETAGIDEVLVRSVLTCEGEHNGICAKCYGRDLARGTEVNIGEAIGVMAAQSIG
                     EPGTQLTMRTFHIGGAAQKGAERSHVDANIDATIEVRNQNVVKNSEGLTIVMGRNTEI
                     VLKDKKGHEKATHRVPYGAKLLVEDGKKVKAGDKMAEWDPYTLPVITEKDGVAHYFDL
                     VDGVSVTDDVDEATGITSKKIIDWRSQPKGGDLKPRISLLDKKGKVINLPNGLPANYY
                     LSVDTILSAENEQEVKAGDIIARIPRETSKTRDITGGLPRVAELFEARMPKDFAVISE
                     IDGYVEFGKDYKSKRRIAVKPANTDDGEPREYLVPKGRHLAVHEGDFVRKGDPILDGA
                     LVPHDILDVMGVEALADYLTNEVQDVYRLQGVKINDKHIEVIVRQMMQKVEITEVGDS
                     TFLVGEHVERSEFDEITAKYESEGKRPPVGKPVLQGITKASLQTRSFISAASFQETTR
                     VLTEAAVQGKVDKLFGLKENVIVGRLIPAGTGAYTAGVKRIAAERDQVILDSQPQPEE
                     EILEGANDAAALSAADTSAPEEEAKEAANG"
     gene            complement(7266..7454)
                     /locus_tag="PK513_05565"
     CDS             complement(7266..7454)
                     /locus_tag="PK513_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81950.1"
                     /translation="MQQVFSVIARPDDEEAATLLENISELHMTNFDGEQAVYSGRGSP
                     EQVNALQAAGNLTVICHE"
     gene            complement(7457..11719)
                     /gene="rpoB"
                     /locus_tag="PK513_05570"
     CDS             complement(7457..11719)
                     /gene="rpoB"
                     /locus_tag="PK513_05570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO81951.1"
                     /translation="MSAAKTTTKTNKQSSAKIPPANNIESFTGRKRVRRSYGRINEVA
                     EMPNLIEVQRHSYELFLQDGIPAEDRTLQGLQEVLATVFPIKDFSDKAEVDFVKYELE
                     APKYDVEECQTRGMTYAAPLRVTLRLSVFDVDEDTGLRSIRDIKEQDVYLVDMPLMTQ
                     NGTFVVNGTERVIVSQMHRSPGVFFDHDGGKTHTSGKYLFAARVIPYRGSWLDFEFDA
                     KDHMYVRIDRRRKLPVTTLLRALDGVETATYRQQCEENDEQVDPLMIQGMANEEILST
                     FYETRIYNKDKKGWKTSFNPEAMRGVKLVADLIDAKTGKVVAEEGDKITPRKAKKLVE
                     DGLKDILVSAEQLVGQYLAADLFDAKTGQVTHEAGHEITEEDLAAINEAGIKELPILA
                     IDHLNVGPYIRNTMLIDKCDTREEALIDIYRVMRPGEPPTVESADALFQSLFFDPERY
                     DLSPVGRVKMNARLDLEADDQFRVLEKADILAILKYLHGLKDGQGEVDDIDNLGNRRV
                     RSVGELMENQIRIGLLRMERAIRERMSSVEIDTYMPHDLINSKPAQAAVREFFGSSQL
                     SQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNI
                     GLINSLATFARVNQYGFIESPYRRVENGKVTDEVVYMSAMEEAKWTIAQANSQLDKSG
                     KFLNDLVSCRRAGENEMTPPDQIEFIDVSPKQMVSVAASLIPFLENDDANRALMGSNM
                     MRQAVPLLKSSAPIVGTGMEGTVARDSGAAVSARRAGVVTKVDATRIVVQATEELHAD
                     EPVVDIYKLAKFQRSNQSTCINQKPLVKVGDKVKAGDIIADGPSTDMGELALGKNILV
                     AFMPWNGYNYEDSILVSERIVRDDVYTSIHLEEFEVMARDTKLGTEEITRDIPNVGEE
                     ALRHLDEAGIVHIGAEVGPGDILVGKVTPKGESPMTPEEKLLRAIFGEKAADVKDTSL
                     RLPPGAVGTIVDVRVFNRRGVDKDARALSIEAQEIERLAKDRDDERRILEDGFYGRLR
                     DILIGNTVASAPKDAKLKKGTKLTAADIDAIEKRGLWRQIAIENEKRMEEIEAMSRTF
                     DDAVDNIKERFENKVEKLQRGDELPPGVMKMVKVFVAVKRKMQPGDKMAGRHGNKGVV
                     SKVIPQEDMPYTEDGTPVDIVLNPLGVPSRMNVGQILETHLGWAAASMGRQIGEIIDA
                     FQSNKKMSDAEKKKLTAKLEDAYGDSEYKGKVSKLDDKELVEMAGNLREGVPMATPVF
                     DGAHDADISELLEKAGLKTSGQVRLIDGRTGDYFHRDVTVGYMYMLKLHHLVDDKIHA
                     RSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGAAYTLQEMLTVKSDDVAGRSK
                     VYESIVRGEDNFEIGIPESFNVLTKELKALCLNVDLKQSSGS"
     gene            complement(12048..>12142)
                     /locus_tag="PK513_05575"
     CDS             complement(12048..>12142)
                     /locus_tag="PK513_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HOO81952.1"
                     /translation="GGNIKEGAKKDEAEEIKKKLEEAGASVALK"
BASE COUNT         2647 a         3164 c         3115 g         3216 t
ORIGIN      
        1 tttaccgcgc cgatgtccaa cggagacggc aaatattcga caacagcatc gagcatcggc
       61 tgaacgcctt tgttcttgaa cgccgttccg cacatgacag gaacgaatgc gcccgcgatc
      121 gtgcctttac ggatgcattt tttcaaggtt tcctcatcgg gttcattgcc ttcgaggtag
      181 gcttccatgg ccgcatcatc ttgttcaaca gccagttcaa ccagcttttc gcggtactca
      241 gcagctttgt cagccagatc ggccgggata tcttcgtaag catatgtcgc gcccagcgcc
      301 tcgccgtccc agacaacggc cttcatgcgc acaagatcca catggccgac ataattgttt
      361 tcggcaccga tcggaagctg gattacgcaa ggcacagcgc cgaggcgatc aatgatcata
      421 tcaactgtgt tgtaaaaatc tgcgccgatt ttatccattt tgttcacgaa acacatgcgc
      481 ggcacatggt atttatcagc ctgacgccac acggtttcgg tttgcggctc aacgccggcg
      541 ttcgcatcaa acacggcaat cgcgccgtca agcacgcgca aggagcgctc aacctcaatt
      601 gtgaagtcca cgtggcccgg cgtgtcgata atattcaggc gaaaacgctg accagagaac
      661 ggaccgtcct tcggcgcgtc ccagaaggtg gtggtcgcag cagaggtaat cgtgatgcca
      721 cgttcctgct cctgctccat ccagtccatg gtggccgcac catcatgcac ttcaccaatt
      781 ttgtgggact taccggaata aaacaaaata cgctcggtcg ttgtggtttt acccgcatcg
      841 atgtgcgcca taatgccgaa gttccggtag ttctcaattg cgtgttcgcg tgtcattttc
      901 agtttccagt tagcggttaa tcagttagcg gtttaccagc ggtaatgcgc gaaggctttg
      961 ttggcctcag ccattttatg cgtatcgtca cgtttcttaa cggcgttgcc acgctcattg
     1021 gctgcatcaa gcaattcgct cgccacgcga tccaccattg tgttttcact gcgtttgcgg
     1081 gcggcatcga taatccagcg catagccagc gccatggcgc gatcgtgacg gacctcgaca
     1141 ggcacctgat atgtcgcacc accaacgcgc cgtgatcgca cttcaagttt cggttttacg
     1201 tttttcagag cattgtggaa cagggccagg cccttgctct tcatcgcaac ggcgacatca
     1261 ccttcaccgg agttcccttc gtcagaagat ttattatcgt taccagcttt ggtttcgaga
     1321 atatcaatcg ctttatagac gatcccttcc gcgatcgatt ttttaccatc gaccatcaaa
     1381 ttattgatga atttcgaaag gaccagatcc gcgaatttcg gatcgggcaa gatttctcgt
     1441 ttttctgctc tgcgtcttct gctcattgtt tttacctatc tatttaattc cacaaatatc
     1501 tggaccataa atttctaaat actctcgcac tgctgccgta taattctcat ggcgaggcgc
     1561 ataaacacta tccaactcca agcgttgttc tgcattaagc cccccgagag ccacagccgc
     1621 cgtgtgctgc gccacaatat ccataccctg ttcaaaatgt ttgtactcag acatctttaa
     1681 aaaccactcc tatttcggac gtttcgcgcc gtagcgtgaa cgggcctgtt tacgatcttt
     1741 aacgccttgg gtatcgagcg ttccacgaat aatggtgtag cgcacaccgg gcaagtcttt
     1801 cacacgaccg ccacgcacaa gaacaacgga gtgctcctgc aaattgtgac cttcaccggg
     1861 aatgtagcaa atcacttcca agcctgtcgt caaacgcacc ttggcgacct tacgcaaagc
     1921 ggagttcggt ttcttcggcg ttgttgtata aacgcgggtg cagacgccgc ggcgttgcgg
     1981 gttgcttttc agcgcccggt ttttctttgc ttttacaact ttttgtctcg gcttgcgcac
     2041 gagctgttga atagttggca tatttctaaa gcctcttttt aaatctcatt tttaaggatg
     2101 gctgcatata gcgtcagaaa gacacgcaag tccagaactt tttttcacaa agtgaaaagg
     2161 actttttatt gcagagcatc ctgtttcttt caggatgttg caccaaacgg gttcaaaccc
     2221 ttaaggccca acagattctg gcgggaatat aggaggaatc gattccggcg tcaagggttt
     2281 attttacgaa tcatcggggc tgatctggtt cagtgttttt ggacaaatca actggccatg
     2341 tatttgaggt tttaggcctc gtcacatgca tgttttcaac ctcgtataaa gctccgattt
     2401 tccatgcatc taagcacctg tgggaagcca gtggtgcaat ggcacttcct tgcgggtcga
     2461 ccgcttctat aaaatctttt gccgcatcat aggacttaaa gcacataaaa ccggcggcgg
     2521 cgcttgtaaa ttcagcgctt atgcgcggga tgttaaagct gtgcgtctct gtctccactt
     2581 gccccgctcc attcggaaga acttcaaata tatacggctt taccgtcacc tcaaggccca
     2641 gatagagggc ctgcggcagt tttttttcct gttcctgtgc ggtgcgaaca agagccttga
     2701 tctttgaaag caccggcaga acaaagttat tttccgcgcc ggattgctgc gctatccctt
     2761 caaagaagga aaaacccata tgttcgacca aagaggcaaa atctggaatg tctattgaga
     2821 gttgttccgg atctgcgctt atttccttta aggttttttc attgaggatg ctcattaacg
     2881 gccctttttg tgtgctgtag gaatgcgcgt ttttagtagc tccattgtta tatgtcaata
     2941 aataaacaaa aaggggctgc aaagcccctt ttcttatatt tttacgattg accggtttgg
     3001 tttaaccgtt ggcggcttct ttggcctctt cttcgggcgc ggacgtatca gcggcggaaa
     3061 gcgcggcggc gtcattggca ccttcgagga tttcctcttc aggctggggc tggctatcca
     3121 ggatcacctg atcccgctcg gccgcgatgc gtttaacgcc cgcagtataa gcacctgtcc
     3181 cagccgggat gaggcggcca acaatgacgt tttctttcag tccaaacagc ttgtcgacct
     3241 tgccttgtac ggcagcttcg gtcagaacgc gtgttgtttc ctggaaggac gcggccgaga
     3301 taaagctgcg cgtttgcaga gatgctttgg tgatcccctg cagaacgggc ttgccaaccg
     3361 gcgggcgctt accttcagat tcgtatttgg cggtgatctc gtcgaattca ctgcgctcga
     3421 catgttctcc gacaaggaat gtcgagtcgc cgacttcggt gatttcaacc ttttgcatca
     3481 tctggcgtac aatcacctcg atatgcttgt cgttgatttt caccccttgc aggcgataaa
     3541 catcctgcac ttcattggtg aggtagtctg ccaaggcttc cacgcccata acatcgagga
     3601 tgtcgtgcgg caccagagcc ccgtcgagga tcgggtcgcc tttgcggacg aagtcacctt
     3661 catgcacggc caggtgacgc cccttcggca caagatattc gcgcggttcg ccatcatcgg
     3721 tgttggccgg cttcacagcg atgcggcgct tggacttata gtcttttccg aattcaacgt
     3781 aaccatcgat ctcggaaatg acggcgaagt ctttgggcat gcgggcttcg aacagctcgg
     3841 ccacgcgcgg cagaccgccg gtaatgtcac gggttttcga tgtctcacgc ggaatacgtg
     3901 cgatgatgtc acccgctttg acttcctgct cattttcggc actcaaaatc gtatccacag
     3961 acagatagta gttcgcaggc aagccgtttg gcaggttaat cactttacct ttcttatcga
     4021 gcaggctgat gcgtggtttc agatcgccgc cttttggctg ggaacgccag tcgataattt
     4081 tcttggatgt aatccctgtt gcttcatcaa catcgtcagt gacggaaacg ccgtcaacga
     4141 gatcgaaata gtgggccaca ccgtcttttt cggtgatgac aggcaatgtg tatgggtccc
     4201 attcggccat tttgtcgccg gctttgacct ttttgccatc ctcgaccaga agttttgcac
     4261 cgtatggaac gcggtgcgtg gctttttcgt ggcctttttt gtctttcagg acaatttcgg
     4321 tattgcggcc catgacgatg gtgagccctt ccgagttttt tacaacgttt tgattgcgca
     4381 cttcgatggt ggcatcgatg ttggcatcaa catgagaacg ctcggcacct ttttgcgccg
     4441 caccgccaat gtggaaagtt cgcattgtca gctgcgtacc aggctcgccg atagattgcg
     4501 ccgccataac gccgatcgct tcgccgatat tgacctcggt gccgcgggcc aaatcccggc
     4561 cgtaacattt ggcgcagatg ccgttatgtt cgccttcaca ggtcaggaca gaacgaacaa
     4621 gcacttcgtc gattccggcg gtttcaattg cttcggtgtt gttttcatca aacatttcgc
     4681 cggctttaac gattgtctta ccgtcaagcg ggttgatgac atcgatagag gcgcagcgac
     4741 ccataatgcg ttcggacaag gatactacca cgtcagcacc gttcataact gcgcgcatag
     4801 aaatgccttt ttccgtgccg caatcttctt cggtaacgat ggcatcctgg gccacgtcaa
     4861 caaggcggcg ggtcaggtaa ccggagttgg ctgttttcag agccgtatcg gccagacctt
     4921 tacgcgcacc gtgcgtggag ttgaagtatt ccagcacgga cagaccttct ttaaagtttg
     4981 agataatcgg cgtttcgata atctcaccag acggcttggc catcaagccg cgcatcccgg
     5041 cgagctggcg gatctgcgct gcagatcccc gtgctccgga gtgagccatc atgtaaacgg
     5101 cattgaggtt gccgctttct atattagaga ttttgctcat catcgcgtcg gctacgtcat
     5161 cggtacattt cgaccagata tcaacgacct tgttgtattt ctcacctttg gtgatcagac
     5221 cgtcttgata ttgctgctca aattccttga ccttgtcatt ggcttcacca accagtttct
     5281 gcttctcatc ggggatgatg aggtcgtctt taccgaagga aatcccagcg atacacgcat
     5341 agcggaagcc gagcttcatc aaacggtcag cgaagatcac agtgtctttc tggccgcagt
     5401 gacgatagga ttcctcgatc acacaggaga gttctttttt ggtcagaacc tggttgatca
     5461 tggaaaacgg aagttccgga tggcgcggca agagatcaga gattaaaatc cgacccggag
     5521 tcgattcaac gatttctgtt ttcgggttac cattttcgtc aactgtgtga taacggcact
     5581 taatttttgc gtgcagggag agtacttttt gttccaaggc atgggtaatt tcacctgcgc
     5641 cgcggaagat cataccttcg ccgggttcgc catcgcgcgc caatgacaaa taatacagac
     5701 ccagaataat atcctgcgtc ggcacaatca cgggtttgcc gtttgcgggc gaaaggatgt
     5761 tgttggttgc catcatcaag cagcgcgctt caagctgggc ttcaatggat agcggcacgt
     5821 gaacggccat ctggtcaccg tcaaagtcgg cgttgaaggc ggtacatacc agcggatgta
     5881 agttgatcgc cttaccttcg atcagtacag gttcgaaggc ctggattcca agtcggtgga
     5941 gcgttggcgc gcggttaagc atcaccgggt gttcgcggat gacttcttcg aggatatccc
     6001 aaacttcggg gcgctctttt tcaaccattt ttttggcagc cttgaccgtg gaggccatgc
     6061 cgtaaagttc aagtttgtgg tagatgaagg gcttgaacag ctcgagcgcc attttcttcg
     6121 gaataccgca ctggtgcagt ttcagctcag gaccgaccac gatgacggaa cggccagaat
     6181 agtcgacgcg ttttccgagc aagttctggc ggaaacggcc ctgtttccct ttgagcatgt
     6241 cagaaagaga tttcaacggg cgtttgttgg cgccggtaat gacgcggcca cggcgaccgt
     6301 tatcgaagag tgcatccacg gattcctgga gcatgcgttt ttcgttccgg acgataatgt
     6361 cgggggcgcg cagttcgatc aggcgtttca ggcggttgtt gcggttgata acgcgacgat
     6421 acagatcgtt caaatcagac gttgcaaaac ggccaccatc caaaggcacc agcgggcgta
     6481 gttcgggcgg cagcacggga atgatatcga ggatcatcca ttcagggcgc gcgccggatt
     6541 cgaggaagct gtcgagcagt ttcaggcgct tgacgagttt cttgcgtttg gcttcggagc
     6601 cggtttcgcg tagatcttct tcgacctgca ccttgatttc ttccagatcg agactggaga
     6661 gaatttcctt catggcctcg gcgccgatac cggcgcggaa attatcttcg ccgtattcgt
     6721 cctgcgcggc gtagtattct tcttcgttca aaagctggta ttgcttgagc ggggtcatgc
     6781 ccggttcgac gacaatgaag ttttcgaaat acaatacgcg ctcaaggtcc ttaagtgtca
     6841 tgtccataat caagccgatg cgcgatggca aggatttcaa gaaccagatg tgagcgaccg
     6901 ggctggccag gtcgatgtgg cccatgcgct cgcggcggac tttggtcagg gtgacttcaa
     6961 cgccacattt ttcgcagatg atgcctttat atttcatacg cttgtatttg ccgcacagac
     7021 attcgtaatc cttgattggg ccaaagatgc gcgcgcagaa cagaccgtct ttttccggct
     7081 tgaaggtccg atagttaatc gtctcaggct ttttgacctc accgaacgac cagctgcgga
     7141 tttgctccgg gcttgcaatc gaaatacgga tggagtcaaa agactgcggc cctgtcggct
     7201 ggccaaagag gttcatgagt tcgttcattt ttcgtgctcc ttaatttggg ctaattttta
     7261 tatctttact cgtggcaaat gacagtcaga ttacctgctg cctgaagtgc attaacttgt
     7321 tctggcgaac cgcgccctga ataaacagct tgctcaccat caaaattagt catatgcaac
     7381 tcagatatat tttccaatag cgttgccgct tcttcatcat ctggtcttgc tattacgcta
     7441 aaaacttgtt gcattactag ctcccgcttg actgtttcaa atcgacattg aggcataggg
     7501 ccttcaattc cttggtcaga acgttgaagg attccggaat accgatttcg aagttgtctt
     7561 cgccgcggac gatggactcg taaactttgg agcgaccggc cacgtcatcg gacttgacgg
     7621 tgagcatttc ctgcaaggtg taagccgcgc cgtaagcctg cagagcccaa acctccatct
     7681 ctccgaagcg ctgacccccg aactgggctt taccgcccag cggttgttgc gtgaccagag
     7741 agtacggccc gatggagcgg gcgtggattt tatcatccac caagtggtgc agtttaagca
     7801 tgtacatgta gcccacggtc acatcgcggt ggaagtaatc tccggtgcgg ccatcgatca
     7861 ggcggacctg accggaggtt ttgagaccgg ctttttcgag cagctcggag atgtcggcat
     7921 cgtgcgcccc gtcaaagaca ggcgtggcca tcggcacgcc ttcgcgcaaa ttgcctgcca
     7981 tttcgacgag ttccttatca tcaagcttgg agaccttgcc tttgtactcg ctgtcaccgt
     8041 aagcgtcttc gagcttggct gtgagttttt tcttctcggc gtcgctcatt ttcttattgg
     8101 actggaaggc atcgatgatt tcaccgatct ggcggcccat agaagccgca gcccagccaa
     8161 ggtgggtttc aaggatctgc cccacattca tccgtgaagg cacgccaagc gggttgagca
     8221 cgatgtcaac gggcgttccg tcttcggtgt agggcatgtc ttcttgcggg atcactttcg
     8281 agaccacccc tttgtttccg tgacgtccgg ccattttgtc accgggctgc attttacgtt
     8341 tcacagccac gaagaccttg accatcttca ttacacccgg aggcagttcg tcaccccgct
     8401 ggagtttttc gactttattc tcgaaacgct ccttgatgtt gtcaaccgca tcgtcaaacg
     8461 ttctggacat ggcttcgatt tcttccatgc gtttttcgtt ttcaatggcg atctgacgcc
     8521 acagaccgcg tttctcgata gcgtcgatgt cagcggcagt aagcttcgtg ccttttttca
     8581 gtttggcatc tttcggcgcg cttgcgaccg tgttgccaat caggatgtcg cgcagacggc
     8641 catagaagcc gtcttccaga atacggcgtt cgtcgtcgcg gtctttggcg aggcgttcga
     8701 tttcctgcgc ctcgatggac aaggcgcgag catctttatc gacgccacgg cggttaaata
     8761 cgcgcacatc gacgatggtg ccaacagcgc cgggtggcag acgtagagat gtatccttca
     8821 catcggcggc tttttcacca aagatggcgc gcaggagttt ttcttccggc gtcatcgggc
     8881 tttcgccttt tggcgtaacc ttaccgacga gaatatcacc ggggccgact tcagcgccga
     8941 tatgcacgat cccggcttcg tcaaggtggc gtagagcctc ttccccgaca ttcgggatgt
     9001 cacgggtgat ttcttcggtg cccagcttgg tgtcgcgggc catgacttcg aattcctcaa
     9061 ggtggattga ggtgtaaacg tcatcacgca cgatgcgctc ggatacgagg atggaatctt
     9121 cgtagttgta gccattccag ggcatgaagg cgacgaggat gtttttcccc agcgccagtt
     9181 cacccatatc ggttgacgga ccatcggcga tgatgtcacc agccttcacc ttatcaccta
     9241 ccttcaccag aggtttctgg ttgatacagg tgctttggtt tgaacgctgg aatttcgcga
     9301 gcttgtaaat gtccacaacc ggttcatccg cgtgcagttc ttccgtggcc tgaaccacga
     9361 tccgcgtggc atcaaccttg gtcaccacac cagcgcggcg tgcagagaca gcagcgccag
     9421 aatcacgagc cacagtgccc tccatgcctg ttccgacgat cggggcagaa gatttgagca
     9481 acggcacagc ctggcgcatc atgttggagc ccatcagcgc acggttggca tcatcgtttt
     9541 ccaagaacgg gataagggat gcggccacag acaccatttg cttcggcgac acgtcgataa
     9601 attcgatttg gtcaggcgga gtcatttcgt tttcaccggc gcggcgacat gatacgaggt
     9661 cgttgagaaa cttgccggat ttatcgagtt gtgagttggc ctgagcgatt gtccattttg
     9721 cctcttccat cgcagacatg tagaccactt cgtcggtgac tttgccattt tcaacgcgac
     9781 gataggggct ttcgatgaaa ccatactggt tgacgcgggc aaaggtggcc agagagttga
     9841 tcaaaccgat gttcggacct tcgggcgttt caatcgggca aatgcggcca tagtgagtcg
     9901 ggtgcacgtc gcggacttca aagcctgcgc gttcgcgggt cagaccgccg ggaccgagtg
     9961 ctgaaaggcg gcgtttgtgc gtaatttccg agagcgggtt ggtctggtcc atgaactggg
    10021 agagctggga tgagccaaag aactcacgca cagcggcttg cgccggttta gagttgatca
    10081 gatcgtgcgg catgtaggtg tcgatttcaa cggaagacat acgctcacga attgcgcgct
    10141 ccatacgcag caggccgatg cggatctggt tttccatcaa ttcaccaaca gagcgtacgc
    10201 ggcggttacc gaggttgtca atgtcatcga cttcgccctg accgtctttc aatccgtgga
    10261 gatattttag aatggcgaga atgtcggctt tttcaagtac gcggaactgg tcgtccgctt
    10321 cgagatcgag gcgcgcattc attttgacac ggccaaccgg cgagaggtca taacgttcag
    10381 gatcaaagaa gagtgactgg aacagcgcat cggcagattc aaccgttggc ggctcaccgg
    10441 ggcgcatgac acgataaata tcgatgagcg cttcttcacg tgtatcgcat ttatcaatca
    10501 gcattgtatt gcggatataa ggcccgacat tgaggtggtc gattgccaaa atcggcaatt
    10561 ccttgattcc ggcttcattg atggcggcca aatcttcttc ggtgatttcg tggccggctt
    10621 catgggtaac ctgaccggtt ttggcgtcaa acaggtcggc ggccaaatac tgaccgacca
    10681 attgctctgc agaaaccaga atatctttca gaccatcttc aacaagcttt tttgctttac
    10741 gtggtgtgat tttatcaccc tcttcggcga cgaccttgcc ggttttagca tcaataagat
    10801 ccgccaccaa ttttacgccg cgcattgctt ctggattgaa tgatgttttc cagccctttt
    10861 tatctttgtt atagatgcgc gtttcataaa atgtgctcaa gatttcttca ttggccatgc
    10921 cttgaatcat caacggatcc acctgctcat cattttcttc acattgctga cggtaggtgg
    10981 ctgtttccac accatcaaga gcacgtaaaa gcgttgtgac cggcaattta cgacggcggt
    11041 caatgcgcac atacatgtgg tccttggcat cgaactcgaa gtctagccaa gacccacggt
    11101 agggaataac gcgcgctgca aacagatatt taccagaggt gtgggttttg ccgccatcat
    11161 ggtcgaaaaa cacgcccgga gagcggtgca tttgagaaac gatgacgcgc tccgtaccgt
    11221 tgaccacaaa tgtaccgttt tgggtcatca gcggcatatc aacgagatag acatcttgtt
    11281 ctttaatgtc gcggatagag cgcaggccgg tatcttcatc aacatcgaag acagacaagc
    11341 gaagcgtaac gcgcagcggc gcagcataag tcatgccgcg agtctggcat tcttcgacat
    11401 cgtattttgg cgcttcgagt tcatatttca caaagtcgac ttccgcttta tcggagaagt
    11461 ccttgatcgg gaaaactgtg gcgagaactt cctgcagacc ttgcagcgta cggtcttcgg
    11521 cggggatacc atcttgtagg aacaattcat aagaatggcg ctgaacttca atcagattcg
    11581 gcatttcagc aacttcatta atgcggccat aactgcggcg aacgcgcttg cggccggtaa
    11641 agctttcaat attgtttgcc ggtggaattt tagctgatga ttgtttgttt gttttagtcg
    11701 tggtttttgc tgcgctcatg atcgatcccg tctttcaatc ttttaaaaat ctttacaagt
    11761 gctttaagct ttttgtctgc aatgcgctgg gcaaaaggca aaaatccaaa gcataaaccc
    11821 accagcatcc tttcccttta agcagaaagg ctgaaagtgg ttaaaactta ccgcaagccc
    11881 aaggctaaag cggaaatccc aaaatattca aactgggttt tcaatgtcat tcccgaaagt
    11941 ttgatggata tatggcctac acgaaccgcc ttgtcaagcg ccgaatagca aaaaagcatt
    12001 ccccgctaaa aagtgcggga aatgcttttg atgtaaataa ggcgtcctta tttaagagcg
    12061 acgcttgcgc cagcttcttc aagttttttc ttgatttctt cggcttcgtc ttttttcgca
    12121 ccttctttga tgttaccacc ag
//
LOCUS       DAOSWR010000056        12064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3354_length_12064_cov_5.667499, whole genome shotgun sequence.
ACCESSION   DAOSWR010000056 DAOSWR010000000
VERSION     DAOSWR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_12064_cov_5.667499"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK513_05580"
     CDS             complement(<1..724)
                     /locus_tag="PK513_05580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO81953.1"
                     /translation="MSIIADRLSRIKPSPTIAVSTKAKELKAAGRDVIGLGAGEPDFD
                     TPDFIKGAAKAAIDAGKTKYTAVDGTPELKDAIIAKFKRDNDLDYVREQITVGTGGKQ
                     ILYNALMASVNPGDEVIIPAPYWVSYPDMTLLAEGTPVIVDCPENNNFKLRAEDLEAA
                     ITPKTKWLIFNSPSNPTGGAYSHDELKALTDVLLKHPHVYVMTDDMYEHLVYDGFEYA
                     TPAQVEPRLYDRTLTVNGISKAY"
     gene            906..1862
                     /locus_tag="PK513_05585"
     CDS             906..1862
                     /locus_tag="PK513_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOO81954.1"
                     /translation="MKDFYNQHKLSIKIVVSLAIMALLAMRMDISALGEHVGHIQGSI
                     WLLAAGMIFVQILALSYRWFLLINAHESRITYRNAVRVTLASMIANYLFITSIGGIIV
                     RVAMSVQSGISLVRSIAATALDRVMTLLALLILTALFLPVLSAIASHDVVHQAVFILS
                     LFASGAFLFALLMFDTFRRRIIFSHRKVAMCFQYLRTVMTDHNMMGKILTSSLVGQVA
                     YFAAVYVVTASMGVEFSWLYFISILPLITVIASLPVGYGGWGIREGAFVYGLGLIDIP
                     IEIAFAVSVQIGLISMLCAFIAGVPALVLRRSSPFKFSVLSR"
     gene            1865..3169
                     /locus_tag="PK513_05590"
     CDS             1865..3169
                     /locus_tag="PK513_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO81955.1"
                     /translation="MMGIARISASLLFLFAVALQIQITLFAADDYIGLRANLADLLLP
                     FAGVLIAGSLLLKKSCWPHWQRPFGYWAPALLSIIIGFALVNGYWAQGGWSHWALFNK
                     GIGWCVLMAYLLAGGWLCTNKPEEILRWFITPFIAFLILAITGDSIVRILYTHGYLPS
                     LSLFGYSFGRDLAGFMVNRNAFAFLYLCALILGSMQLALHPALNRLQKIGFMALWFLL
                     PLFLVLNLSRASLLVLVPLALYLLISQRCLFLRVCLPLTLAGLLLVPFANFANVENAL
                     HNLGKISHVQAHVKNEGSLPYEESVYRGDQVRIQIIQDSWGLIKEHPLTGAGIGTILK
                     LQQDEGRQYVSVIDNTPLWLLTEMGPFGLLGFVSVYVSILLTLLRKRRDADPENQAFI
                     MSAVLILLAFGIYSLLHEILYARFFWFILGMSLALPTARLQQ"
     gene            complement(3147..4190)
                     /locus_tag="PK513_05595"
     CDS             complement(3147..4190)
                     /locus_tag="PK513_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOO81956.1"
                     /translation="MSQALQNLLFDEDHRQMFLKKHGWEIMHSVGQDSSIRRYFRVSK
                     GSQNAILMETVPDGSTHATPGHSLDDFIRIASWLRDIGLKAPEIYEQDLKNGYLLLED
                     LGDLCFRDALDSGEDASMLYGLARDILDHIAAQNCSLDLPDYYDAHVHRRHRRIVDWF
                     IPLMRGTPNEEGLVEEYLTVWRGIESSAPPVRQGFLHIDFHAQNLMWLPSETGLKRCG
                     ILDFQGAMLGPRPYDLGNILEDARRDVPAEVKSHILDQLSEDERIWFRILATQFHCRV
                     IGQFIKQAVIDENPAYLKHVPRLQNYLANGPKDPILTPLARWFTQQRIDFSSAASLEN
                     LEILRPLIAEDAQ"
     gene            complement(4215..4634)
                     /locus_tag="PK513_05600"
     CDS             complement(4215..4634)
                     /locus_tag="PK513_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81957.1"
                     /translation="MSLEYAENRIKEALKLSGGNQIKARQQIIAWTFEDAKLLHALAK
                     PHLSGIVAYNIERVASGRADAARAKAGLKPEKPQATQKSKASNEEAFGFEILKAIAGS
                     SQMFGLEDAGAPRKRAPTSQAHVDALRAIASKQKKTD"
     gene            complement(4717..5526)
                     /locus_tag="PK513_05605"
     CDS             complement(4717..5526)
                     /locus_tag="PK513_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOO81958.1"
                     /translation="MNAHKNIKPDNHVRVGILAERNENGEVSICERWARPFEKAGMNV
                     EYIPVGAENIDEILDRIHGLLLPGGDSNINPYFYDPGQLNDHLSIAQQRDIARDRTAI
                     ALAQKAYALNIPTLGICRGMQEMLVAFGGALETLNTQINHAQGYNAVLADGTRCFETM
                     DRPVHEIEIVPGGLLDSIYDKKNFFVNSIHMQGITHEFWQHDRNKDLREVFRVEAYAP
                     DGVIEAISTHREHEKRFFVGLQAHFELPGALHISIFTPFRREIRDYAANKS"
     gene            complement(5567..9076)
                     /gene="mfd"
                     /locus_tag="PK513_05610"
     CDS             complement(5567..9076)
                     /gene="mfd"
                     /locus_tag="PK513_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOO81959.1"
                     /translation="MAQTAENIEVSQNVIYGAVEGQDARLLAARAREIMPADKVLVHI
                     ALDDARMAILGELLAFFAPDAHVIEFPAWDCLPYDRVSPNADIVVKRVTALTQMLNWE
                     KESKRYPRIVLTTINAVTQRVMPKDVLDGASFAAQKGSPLNVAALQNFLTQNGYTRTQ
                     TVREAGEYAMRGGIIDLFPPGYSDPLRLDLFGDEIETIRSFDPLTQRTHTDVKSLQLR
                     PVTEFFLNEESIDRFRSSYREAFGVVQGSDPLYESVSEGRRYNGMEHWLPLFYEGMST
                     VLDYAGQYEVILDHHARRAEGERLKQIRDFYQSRQTLKDAALKKKKKSKDISLSGAFY
                     NPLPPERLYLGEIEWAATVADAQLLSPFGAPGQGHSPVKKARDFADIRALPDGDVIDE
                     LKTHIAGLANGERKILIACFSEGSKQRLQGLMEEDGIGPIKAVTNAEDFKKLKPRDIG
                     FVILGLEHGFTSDDLAVLTEQDILGDRLAHKSNRRKKAENFLREVSSLDEGDLVTHID
                     HGIGKFLQLETLEAAGTLHDCLKIEYAGGDRLFVPVENIEVLSRFGADEGAVQLDKLG
                     GAGWQARKARVKKDLMAMADGLLKIAAERQLRKTQPIEVSKELYHEFASRFPYAETDD
                     QERAINDVLESLAGSHPMDRLVCGDVGFGKTEVALRAAYVAAMDGFQVAIVAPTTLLA
                     RQHYLNFTKRFASTNLRIKQLSRLVSTKEANTIKDGLTDGSVNIVIGTHALFGKGIKF
                     SNLGLLVVDEEQRFGVKQKERLKELKNNVHVLTLTATPIPRTLQMSLTGVKEMSLITT
                     PPVDRLAVRTFVMPTDTHVIREALLREHYRGGQSFYVCPRIADMDELEDMLKDLVPEV
                     KIITAHGQMSPTELEDRMSAFYEGRYEVLLATNIIESGIDIPRANTLVIHRADMFGLA
                     QLYQIRGRIGRSKMRAYAYLTYPANAPLNPQAQKRLEVMETLDTLGSGFQLASHDMDI
                     RGAGNLLGDQQSGHIKEVGVELYQQMLEEAVAIAREGVSLDNMPAQDEGWSPTINLGA
                     SVLIPETYVPDLGVRMSLYRRLTDLLESQDIEAFAAELIDRFGDLPEEVQNLLDIVAI
                     KQLCKKAGVSSVEAGPKGAVIGFHNNTPPNIEGLMHWMTSKGGALKMRPDHKLVAIRQ
                     WDSVARRVKGVQNLMKELMEITA"
     gene            9162..10442
                     /locus_tag="PK513_05615"
     CDS             9162..10442
                     /locus_tag="PK513_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOO81960.1"
                     /translation="MTQPTATNENEKPDGALLKTAVHEHKAISKRGFLERLFTLSFRG
                     FVYPQIWEDPEVDLAALKLDKTSRVMTIASGGCNVMNYLTESPDKVKVIDLNPAHVAL
                     TRLKIAAIKHLPDYESFFAFFGHADEKRNIENYDTYIAPHLDAFSKKYWEGWSPLRGR
                     RINYFTKNLYQFGLLGRFISLVHILAKIYGQDPREILEAKTQKEQGEIFDRTLGPIFE
                     KKFVRFICNMPPALYGLGIPPSQFDDLKGEADMADVLKARLRRLAVDFPIEDNYFAWQ
                     AFGRGYDRAYKKAVPRYLKEEYYETLKANVDKVEVHHTTITGFLQSQPASSLDCYVFL
                     DAQDWMNDKALNVLWSEVVRTACPGGRIIFRTAGDESPLTNALSKDILEKFDYDPTLS
                     AEAVKKDRSSIYGGFHVYTFKAGKEAAAKKKKAA"
     gene            10446..11102
                     /locus_tag="PK513_05620"
     CDS             10446..11102
                     /locus_tag="PK513_05620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO81961.1"
                     /translation="MSEEADTKDEMNRMYRWTRHVYDASRKYFLLGRDQLINTLRLKD
                     GEHVCEVGCGTARNLIKMARRYPKTHFYGLDASDEMLKTARGSLKFSGLSERVHVKQS
                     FAQNFDPQELFNLSAPLDKILFSYALSIIPPWQDSIDHAVQLLATGGEIHIVDFGDMK
                     DQPAWFKKIIFWWISLFHVYHKPEILEHLQDLVRNGQGTLNITHLYNGYAYLAVFKKT
                     "
     gene            complement(11099..11362)
                     /locus_tag="PK513_05625"
     CDS             complement(11099..11362)
                     /locus_tag="PK513_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOO81962.1"
                     /translation="MTVESLENKRKRLIFRSEHRGTKEMDLLLGSFARKYVPDFSEDE
                     LSQYDEILKENDPDLYNWITGKNEAPANIMNPVFKRLMKHRYA"
     gene            11394..11984
                     /locus_tag="PK513_05630"
     CDS             11394..11984
                     /locus_tag="PK513_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81963.1"
                     /translation="MKAFLKENMVLAAGIALPLLLALIFFGATQMGKATIDPPHYSVI
                     FAADYYHNNNNYPYNLVIEKDQLRFVYIPPEEKDSYHNWQKPRLFVYNPVKNESHEVA
                     LPNIKDKKKKIDEVIEDIHAQKLSSLKESPDGFVFDHDYRGGGNLMTELFGGGYRSRS
                     RHVLRKASYSVPVPKSERYNSEFIGWIIEPAETADD"
BASE COUNT         2837 a         2738 c         3151 g         3338 t
ORIGIN      
        1 cgtaggcttt ggatatgccg ttaacggtca gggtgcggtc ataaagcctg ggttcaacct
       61 gggcaggcgt ggcgtattca aacccgtcat agacgaggtg ctcatacata tcatcggtca
      121 taacgtagac atgcgggtgt ttgagcaaca cgtcggtgag cgctttaagc tcgtcatggc
      181 tataggcgcc gccggtcgga ttggagggcg agttaaagat cagccatttt gttttcggag
      241 tgatcgcagc ctcgagatcc tccgcacgaa gtttgaaatt attgttttcc ggacaatcaa
      301 caataacagg cgtgccttca gccaacagcg tcatatcggg gtatgacacc caatacggcg
      361 cggggataat cacttcatcc cccggattca cagaggccat cagcgcattg taaagaatct
      421 gtttcccgcc ggtgccgacg gtgatttgtt cgcgaacata atccagatcg ttatcgcgtt
      481 tgaactttgc aataatcgca tctttgagtt ccggcgtacc gtccacggcg gtatatttgg
      541 ttttgcctgc atcaatggca gccttggcag cgcccttgat aaaatctggc gtatcgaagt
      601 ccggctcacc ggctcccagg ccgatcacgt cgcgcccggc ggctttaagc tccttggcct
      661 tggtcgatac ggctatggtc ggtgaaggct taatacggct gaggcgatcg gcaatgatgg
      721 acatagtttt tcctttttct tgttcatatt tcttgttcat agccatgttt ttatttagct
      781 tgtttcacat agatgaatct ttttgtgtta gagtcttacc tcttgttgta agaatttccc
      841 attggattgg caagttccgt attgtattcc ttttttaatc gctgaaatgt aaatcagaaa
      901 cccacatgaa agatttttat aaccagcaca agctttccat taaaatcgtc gtttcattgg
      961 cgattatggc gctgctggct atgcggatgg atataagcgc gcttggtgag catgttggcc
     1021 atattcaggg ttcgatctgg ttgctggctg caggaatgat ttttgtacag attttggcac
     1081 tgtcttatcg ctggtttttg cttataaacg cccatgaaag ccgaattact tatcgtaatg
     1141 cggtgcgtgt aacactggcg agtatgattg ctaattatct gttcattaca tcaatcgggg
     1201 ggatcatcgt acgcgtggca atgagtgtgc aaagcggaat ctcgctggtg cgctcgattg
     1261 ccgcgaccgc gcttgaccgg gtgatgacct tgcttgcgct gctcatcctt accgctttat
     1321 ttctgccggt gctcagcgca attgccagtc atgatgttgt gcatcaggcc gtgtttattt
     1381 tgagcctctt tgccagcggt gcgtttctct tcgctttgct gatgtttgac acattccggc
     1441 ggcgaattat tttctctcat cggaaagtgg cgatgtgctt tcaatatttg cgtacggtga
     1501 tgacggacca caacatgatg ggcaagattc ttaccagcag tctggttggg caggtggcct
     1561 attttgctgc tgtatatgtg gttacggcct caatgggcgt agaattttcg tggctatatt
     1621 ttatttcaat tctgccgttg attacggtca ttgccagctt gcccgtcggt tatggcggtt
     1681 ggggaattcg tgaaggtgcc tttgtttatg ggctggggct gattgatatt cccattgaga
     1741 ttgcttttgc cgtatccgtg caaatcggtt taatcagtat gctctgcgcg tttatcgctg
     1801 gcgtccctgc gctagtcttg cggcggagtt caccctttaa gttttcggta ttatcacgtt
     1861 aaagatgatg ggcattgcgc gtatttccgc ttcgctactt tttctttttg ctgttgcatt
     1921 gcaaattcaa atcactttat ttgccgctga tgattatatt ggtctgcgcg ccaatctggc
     1981 cgatttgcta ttaccgtttg cgggcgtttt aatcgcggga agcttgctgt tgaaaaaatc
     2041 atgctggcca cattggcaac gaccgtttgg atattgggcc cctgcccttc tcagcattat
     2101 tatcggcttt gcactggtga atggatattg ggcgcagggc ggctggagtc attgggcgct
     2161 gtttaacaag ggcatcggct ggtgcgtatt gatggcgtat ttactagccg gaggatggct
     2221 gtgcaccaac aagcctgaag aaattctgcg ctggtttatt acaccgttta ttgcctttct
     2281 gatccttgca attacaggcg acagcatcgt acgcattctt tatacccatg gctatctgcc
     2341 atcattatcg cttttcggtt atagttttgg gcgagatctg gccggtttta tggtcaaccg
     2401 aaacgcgttt gcgtttttat atttatgcgc actgatcctc ggcagtatgc aattggcgct
     2461 gcatccggcg cttaaccggc tacaaaagat cgggtttatg gcgctgtggt ttttattacc
     2521 tttatttctt gttttaaatc tatcacgggc ttctttgctg gttttggttc cgctagcgtt
     2581 gtatctgctt atatcccaaa gatgcctttt tttgcgggtt tgtttgcctc tgacgttagc
     2641 aggactgttg cttgtgcctt ttgcaaattt tgcgaatgtc gaaaatgctc tgcacaatct
     2701 tggcaaaatt tcgcatgtgc aggctcatgt caaaaacgaa ggttcattgc cctatgagga
     2761 gtctgtttat cgcggtgatc aggtgcgaat tcagattatt caagatagtt ggggattgat
     2821 caaagaacat ccacttaccg gcgcagggat tggaactata ttaaagctgc aacaagatga
     2881 agggcggcag tatgtatcgg tgattgataa tacgccgctg tggcttttga ccgagatggg
     2941 gccgtttggc ttgctggggt ttgttagcgt ttatgtttcc atattactga ccctgcttcg
     3001 taagcgccgt gatgctgatc cggaaaatca agcctttatc atgagcgcgg tgttgattct
     3061 tctggcgttt gggatttatt ctctgctgca tgaaattctt tatgcccggt ttttctggtt
     3121 tattttgggg atgagtttag ccttgcctac tgcgcgtctt cagcaataag ggggcgaagg
     3181 atttctaaat tttccagcga tgcggcagat gaaaaatcta tcctctgctg cgtgaaccaa
     3241 cgggcaagcg gcgtgaggat cggatctttc ggaccattag ctagatagtt ttgcaggcgc
     3301 gggacatgct ttaaataggc gggattttca tcgattacgg cctgcttgat gaattgaccg
     3361 ataacgcggc aatggaactg cgtagcgaga atgcgaaacc agatgcgttc gtcttcactt
     3421 aattgatcaa gaatgtggct ttttacctct gcaggaacat cgcggcgcgc atcttcgagg
     3481 atattaccaa gatcgtaggg acgggggccg agcatagccc cctgaaaatc gagaatgccg
     3541 cagcgtttta ggcctgtttc actgggcaac cacatcaaat tttgtgcatg aaagtctata
     3601 tgcagaaacc cttgccggac tggaggagca ctggactcaa ttccccgcca aacagtgagg
     3661 tattcttcaa ccaacccttc ttcattaggc gttccgcgca ttagcggtat aaaccaatca
     3721 acgatgcggc gatgacggcg atgaacatga gcgtcgtaat aatcggggag atccagcgaa
     3781 caattttgcg cagcgatatg gtccagaatg tcgcgtgcca agccgtataa catggacgcg
     3841 tcttcgccag aatctaacgc gtcgcgaaaa cataaatcgc caagatcttc gagcagcaaa
     3901 tatccgtttt tcaggtcttg ttcataaatc tcgggggcct taaggccaat gtcgcggagc
     3961 caactggcga tgcggataaa atcatcaagg ctgtggccag gcgtggcatg ggtagagccg
     4021 tcgggaaccg tttccattaa aattgcgttt tgcgatcctt tggatacccg gaaataacgg
     4081 cggatagagg aatcctgtcc aactgaatgc attatttccc aaccatgttt ttttaaaaac
     4141 atctggcgat gatcctcgtc aaagagaaga ttttgtaatg cttgagacac gccggtctcc
     4201 tttgattaat gacgttaatc agttttcttt tgtttggatg ctattgcgcg cagggcatcg
     4261 acgtgggcct gtgaggttgg tgcgcgcttg cgcggcgcgc ctgcatcttc gagaccgaac
     4321 atctggctgg agcctgctat agctttaaga atttcaaagc caaaagcttc ttcatttgaa
     4381 gcctttgatt tttgagttgc ctgcggtttt tccggtttca atcctgcctt ggcacgtgcg
     4441 gcatcggcgc gccccgaagc cacacgctct atattatagg ccacaatgcc ggaaaggtgc
     4501 ggcttggcca gcgcgtgaag cagcttggca tcttcgaagg tccaggcaat gatctgctgg
     4561 cgggctttga tctgattccc gccactgagc tttaaagcct ctttaatacg gttttcggcg
     4621 tattctagtg acatacgcgg cctccttttc gggctgatga cctttcattc cttatactct
     4681 gtcaggatga catattttgc aagattgacc aagctcttaa gatttattgg cggcataatc
     4741 acgaatttca cggcggaatg gggtgaagat tgaaatgtgc aatgcacctg gtaactcgaa
     4801 atgagcctgc agacctacaa aaaatcgctt ttcatgttcg cgatgggtgc taattgcctc
     4861 aatcactccg tctggggcat aggcttcgac tctgaaaacc tcccgtaaat ctttattgcg
     4921 atcatgttgc cagaattcat gggtgatgcc ttgcatatgg atggagttta cgaagaagtt
     4981 tttcttatca tagatgctat ccagaaggcc gccaggcacg atttctattt cgtggacggg
     5041 gcgatccatg gtttcaaagc agcgggtgcc gtcggccaga acagcattat agccttgggc
     5101 gtgattgatt tgagtattca gcgtttcaag cgcaccgcca aaggccacaa gcatttcctg
     5161 cataccgcgg caaataccca atgtcgggat gtttaaggcg taggcttttt gggccaatgc
     5221 aatggctgtg cggtcgcggg caatatcgcg ctgctgtgct atggagagat ggtcattgag
     5281 ttggcctgga tcgtagaaat acggattgat gtttgaatca ccaccgggaa gcagcaagcc
     5341 atgaatacgg tctaagattt catcaatatt ttcagcacca acgggaatat attcaacatt
     5401 catccctgct ttttcaaatg gacgcgccca gcgttcacaa atagatacct caccattttc
     5461 atttctttcg gcgaggatgc caacgcggac atgattgtcc ggttttatat tcttgtgggc
     5521 gttcatgatg tctcttttta tgctaaaata ttaaaaaaca ataaaattat gcggttattt
     5581 ccatcaattc tttcatcagg ttttgcacac ctttgacgcg ccgggcaacg ctgtcccact
     5641 ggcggatggc aacgagtttg tgatctgggc gcattttcag ggcgccgcct ttggacgtca
     5701 tccagtgcat caagccttcg atattaggcg gagtgttatt gtgaaaaccg ataactgcac
     5761 cttttggccc tgcttctacc gatgaaactc cggctttttt acagagttgt ttaatggcga
     5821 caatgtcgag cagattttgc acttcttcgg gaagatcgcc gaaacgatca atcaattcag
     5881 cggcgaaggc ttcgatgtct tgcgactcta aaagatcagt gagacggcga tacaggctca
     5941 tacgcacgcc aagatccggc acataagttt cgggaataag cacggacgcc ccgagattga
     6001 tggtgggcga ccagccttca tcctgcgcag gcatattatc gagcgaaacg ccctcgcggg
     6061 ctatggcaac cgcttcttcg agcatttgct ggtatagctc gacaccgact tccttgatat
     6121 gaccggattg ctgatcgccg agcaggtttc cggcaccacg gatgtccatg tcgtgagaag
     6181 caagttgaaa accactgccg agcgtatcga gcgtctccat gacttcgagg cgtttttgcg
     6241 cttgaggatt taatggcgcg ttagcggggt atgtgagata ggcataagcg cgcatctttg
     6301 aacggccaat gcgtccacgg atctggtaga gctgtgccaa gccgaacata tcggcgcggt
     6361 gaattacgag tgtattggcg cgtggaatgt cgatgccgct ttcaatgata ttagtagcaa
     6421 gcaagacttc gtagcggcct tcgtaaaaag cgctcatacg atcttcaagt tcggttgggg
     6481 acatttgccc gtgggcggtg ataattttaa cctcaggtac gaggtctttg agcatatctt
     6541 caagctcatc catgtcggcg atgcgcgggc aaacgtaaaa actctggccg ccgcgataat
     6601 gttcacgtag cagcgcttca cggataacat gcgtgtcggt gggcatgacg aaggtacgca
     6661 cggcgagacg gtcaacgggc ggggtcgtga tgagcgacat ttccttgacg ccggtaagtg
     6721 acatttgcag cgtgcgcggg ataggcgtgg ctgtcaaagt gaggacatgc acattgtttt
     6781 ttagctcttt gaggcgctct ttttgtttga cgccgaaacg ttgttcttca tcaacaacca
     6841 aaaggccgag attgctgaat tttataccct tgccgaacaa cgcatgcgta ccgatgacga
     6901 tattgacgct accgtcggtg aggccgtctt tgatggtatt ggcttctttt gtagagacaa
     6961 gccgcgagag ttgtttgata cgcaagtttg tgctggcaaa acgtttggta aaattaagat
     7021 agtgctggcg tgcgagcaag gtggtcggtg caacaatagc aacctgaaaa ccgtccattg
     7081 cggcgacata ggctgcgcgc agggcaactt cggttttacc aaagccgaca tcgccgcaaa
     7141 caagacggtc catggggtgc gacccggcaa ggctttccag cacgtcgtta atggcgcgtt
     7201 cctgatcgtc agtttccgcg taaggaaagc ggctggcgaa ttcgtggtat agttctttgg
     7261 agacttcgat aggttgtgtc ttacggagtt ggcgttcggc tgcgattttg agaagaccat
     7321 cggccatggc catcaaatct tttttaacgc gggctttgcg ggcctgccat cctgccccac
     7381 cgagtttatc aagctggacc gcaccttcat cagcaccaaa gcgtgagagg acttcgatat
     7441 tttctaccgg tacgaagaga cggtcgccgc cggcatattc gatcttgagg caatcatgca
     7501 gagtgcctgc ggcttccaga gtttcgagtt ggaggaattt gccgatgccg tggtcgatat
     7561 gggtgacaag gtcgccttca tcaagacttg agacttcgcg taaaaaattt tcggcttttt
     7621 tgcgccgatt gctcttgtgt gcaaggcggt cgccgaggat gtcttgctcg gtcaacaccg
     7681 caagatcatc ggacgtaaac ccgtgctcaa gacctaaaat gacaaagccg atatcgcgag
     7741 gtttgagctt tttaaaatct tcggcgtttg tcaccgcttt gatcgggccg attccatctt
     7801 cttccatcaa cccttgcaag cgttgctttg agccttcgga aaaacaggcg atgaggattt
     7861 tacgctcgcc gttggcgagg ccggctatgt gagtttttag ttcgtcgata acgtcgccat
     7921 cgggaagcgc acggatgtcg gcaaagtcgc gggctttttt gacgggggaa tgtccctgcc
     7981 ccggcgcacc aaaaggggaa agaagctgtg catctgcaac cgtagcagcc cattcgattt
     8041 cgcccagata taatcgttca ggtggcaacg gattatagaa cgcaccggaa agactgatgt
     8101 ctttggattt tttcttcttt ttgagggctg cgtctttgag ggtttgacgt gactgataga
     8161 aatcgcgaat ttgcttgagg cgctcccctt cggcgcggcg ggcgtggtga tcgagaatta
     8221 cttcatattg cccagcataa tcgagcactg tgctcatgcc ttcataaaag agcggcaacc
     8281 aatgttccat accattataa cgccgccctt ctgagacgga ttcgtacaaa ggatcggagc
     8341 cttgtacgac accaaaggct tcacgatagc tgctgcggaa acgatcaatg gattcttcat
     8401 tgaggaagaa ttcagtgacg ggccggagct ggaggctttt aacatctgtg tgggtgcgtt
     8461 gcgtcagcgg atcaaagctg cggatagttt caatctcatc accaaacaga tcaaggcgca
     8521 gtggatcgct atagccgggc ggaaacagat caatgatgcc gcctcgcatg gcgtattctc
     8581 ccgcctcgcg cacggtttgc gtgcgggtat atccattctg tgtgagaaaa ttttgcagcg
     8641 cggctacatt tagagggctg cctttttgcg cggcaaaaga cgcaccgtcc agtacatcct
     8701 tgggcataac cctctgcgtt acggcattga tggtggtcaa aacaatgcga ggataacgct
     8761 tggattcttt ctcccagttt agcatctgcg tcagcgctgt gactcttttg acaacaatat
     8821 ctgcatttgg cgacacacgg tcatagggca aacagtccca agcaggaaat tcgattacat
     8881 gcgcgtcggg tgcgaagaaa gccagaagtt ctcccaggat agccatgcgc gcatcgtcga
     8941 gtgcgatatg aaccagcact ttatcagcag gcataatctc acgcgcacgc gcggccagca
     9001 accttgcatc ctgtccttct actgcgccgt aaatgacgtt ttgagaaact tcaatatttt
     9061 ctgcggtttg agccataaat cccttataaa gccattgtgc tgccgggttt ttatatatag
     9121 gaataacgaa gacttaaaga gagaattaag gattagtttt tatgacgcag ccaaccgcaa
     9181 ccaatgaaaa tgaaaagccc gacggtgcat tgctaaaaac agcggtgcat gaacacaaag
     9241 ctatcagcaa acgtggtttt ttagagcggt tgtttacgct ttcgtttcgc ggatttgtct
     9301 atccgcagat ctgggaagat ccggaagttg atcttgcggc gctgaagctt gataaaacca
     9361 gccgggttat gacgattgct tcgggcggat gtaatgtgat gaattacctg accgaaagcc
     9421 cggacaaggt caaggttata gaccttaacc cggcgcatgt ggcgttgacc cggcttaaga
     9481 tcgccgcgat taagcatctg ccggattatg aaagtttctt tgcgtttttt gggcacgctg
     9541 atgaaaaacg taatattgaa aattacgata catacattgc gccgcatctg gatgcttttt
     9601 ccaagaaata ctgggaaggg tggagtccat tgcgaggtcg gcgtatcaat tactttacca
     9661 aaaatttgta ccagtttgga ttgctggggc ggtttatttc gctcgtgcat attctggcaa
     9721 aaatttacgg gcaggatcca cgggagattt tagaagccaa gacacaaaag gaacagggtg
     9781 aaatttttga ccgcactttg gggccgatct ttgagaagaa gttcgtgcgc tttatttgta
     9841 atatgcctcc tgccctgtat ggtcttggca ttcccccgtc acagttcgat gacctgaaag
     9901 gtgaggccga catggccgat gtcctgaaag cgagattgcg ccgtctggcc gtggattttc
     9961 caattgagga taattatttc gcctggcaag cctttgggcg gggctatgat cgtgcgtata
    10021 aaaaggctgt gccgcgctat ttgaaagagg aatattacga aacactgaaa gccaacgtgg
    10081 ataaagttga ggttcaccac acaacaatta ccgggttttt gcaatcacag ccggcaagca
    10141 gtctggattg ttacgtcttt ctggatgcac aagactggat gaatgataag gccctgaacg
    10201 ttctgtggag tgaggttgtt cgcacggcgt gtccaggcgg gcgcattatc ttccgcactg
    10261 ccggggacga aagcccgctc accaacgcgc tttcaaaaga cattttggaa aaatttgatt
    10321 atgacccgac actctctgcc gaagccgtga agaaagaccg ctcgtcgatt tatggcgggt
    10381 ttcatgtcta tacttttaag gctggaaaag aagctgcagc aaaaaagaaa aaagcggcat
    10441 aggacatgag tgaagaggca gacacaaaag acgaaatgaa ccgcatgtat cgctggacgc
    10501 gtcacgttta cgatgcgagc cggaaatatt tcctgctggg ccgcgaccag ttgatcaata
    10561 cattgcgtct gaaagacggc gagcatgttt gtgaagtagg atgcggaaca gcgcgcaacc
    10621 tgatcaaaat ggcaaggcgt tatccgaaaa cacattttta tggtctggat gcttcagatg
    10681 aaatgctcaa aaccgcacgc ggatctttga aattttcggg gctgagtgaa cgcgttcatg
    10741 tcaaacaaag ttttgcgcag aattttgatc ctcaagagtt atttaatctt agcgctccgc
    10801 tggataaaat cttgttttcc tacgcgcttt ctattattcc gccatggcag gactctatcg
    10861 atcatgcggt gcaactgctg gcgacgggcg gtgaaatcca tattgttgat tttggcgata
    10921 tgaaggacca gcccgcgtgg tttaaaaaaa taatattctg gtggatttcc ctgttccatg
    10981 tctatcacaa gcctgaaatt ttagagcatt tgcaagatct tgtccgcaac ggccagggca
    11041 cgctcaacat tacccatctg tataacggtt atgcgtatct ggcggtattt aagaaaacct
    11101 aagcgtagcg gtgtttcatt aatcgtttga atacgggatt cataatatta gcgggggctt
    11161 cgttcttacc ggtgatccaa ttataaagat cgggatcgtt ttcttttagg atttcatcgt
    11221 attgtgacag ctcatcttcg ctgaaatccg ggacatattt gcgggcgaag gagccgagca
    11281 agaggtccat ctccttcgtc ccgcgatgtt cggaacgaaa gatcaggcgc ttgcgtttat
    11341 tttcaagcga ttctactgtc ataaaagcga gtttaataag gaaaggcgcg taaatgaaag
    11401 cttttttaaa ggaaaatatg gttttggcgg cgggtatcgc cctgccgtta ttgttggcct
    11461 tgatcttttt cggcgcgacg cagatgggca aggctaccat agatccgccg cactacagcg
    11521 ttattttcgc ggcggactac tatcacaaca ataataacta tccctacaac ctggtaatcg
    11581 aaaaagatca gttgcgcttt gtgtatatcc ctcctgaaga aaaggattcc tatcacaact
    11641 ggcaaaaacc ccggctgttt gtttacaacc cggttaaaaa tgaaagccat gaggttgcat
    11701 tgcccaacat taaggataag aaaaagaaaa tcgacgaggt cattgaagac attcacgctc
    11761 aaaaactctc ttccctgaag gaaagtcctg acggtttcgt ttttgatcat gattaccgcg
    11821 gcggcggtaa tttaatgaca gagctttttg gcggaggtta ccgctcacgc tctcgtcatg
    11881 ttttaagaaa agcctcttac agcgtccctg taccgaagtc agagcgttat aactcggagt
    11941 ttattggctg gattatcgag ccggcggaga ctgctgatga ctagtgacgc gatgagcaaa
    12001 aaagacgcgc tggagcaaat cgtagctttg gcgcgggcgc atgatattac gctggatgaa
    12061 atcg
//
LOCUS       DAOSWR010000057        12009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3378_length_12009_cov_4.872511, whole genome shotgun sequence.
ACCESSION   DAOSWR010000057 DAOSWR010000000
VERSION     DAOSWR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3378_length_12009_cov_4.872511"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PK513_05635"
     CDS             complement(<1..1169)
                     /locus_tag="PK513_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO81964.1"
                     /translation="MLFGHIQNHDPEKIALCDEMQTVCYGDLKREIQKRMDALYGVSV
                     LGIALDNSVDWVLWDLAALKAGIPCVPLPPFFTEQQAQHTLHSAGVTHVLFPKELRDT
                     GLKGLAFLPEGTAKVTFTSGTTGTPKGVCLSQKGLEQVACSIVKALGTELADKHLSVL
                     PLAILLENVAGVYATLMAGGTVYLPSLKMTGFANPFEPDFAALSHYMGKHEITSAILV
                     PELLRGLMSVRPDLPHLKFLAVGGAKVSPSLISAARKMGLPVYEGYGLSECASVVSLN
                     TPENDKPGSAGKILPHIRLVENDGEITIKDPAFLGYLGNKHKGEFATGDLGYVDEDGF
                     LHINGRKKNVLITSYGRNISPEWVEAALLAQPEIMSAVIYGDAGPFKRGNRSGLFA"
     gene            complement(1169..1873)
                     /locus_tag="PK513_05640"
     CDS             complement(1169..1873)
                     /locus_tag="PK513_05640"
                     /inference="COORDINATES: protein motif:HMM:NF023682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HOO81965.1"
                     /translation="MLKLIYQENNIDTASLYLREDRTRRISSLTPSVVSITSLFEPER
                     QRVEDFIRAIYKQSYNADIEVNYPVLMSVRNANGDILAAVGFRYAKDESLFLEHYTQG
                     SVEKLLDCPRNEIVEIGNLASAGQGASAFLFAALASYLNNKDIRYAAITGTDFLHRYF
                     ERVGLKPRKICDADIKAVEHDTQNWGSYYDTQPRVLVGSVETGVKRLKAMLGAEFEDC
                     RPRLFPRLHYKQEGRN"
     gene            2281..4929
                     /locus_tag="PK513_05645"
     CDS             2281..4929
                     /locus_tag="PK513_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOO81966.1"
                     /translation="MTCFHALSAEETIKSLETSAKNGLTESEIQVRQSQYGSNTLIEK
                     ESVKWPLVLIRQFTSFLILILLVAAALSFFIGDELDTLAIFAIVILNGLLGFAQEWKA
                     ETALKSLKKMLSPRCRVIRDSRMQEIDAAALVPGDIVLLESGDSVPADIRLLDEVNLR
                     ADEAALTGESVSVAKNIHAISKDSPISWRTCMVWMGTHIVNGRGQGVIVATGMETEFG
                     RIAKLTGDIQETQTRLQRQLAVLAKQLTVVIVGVTAVVMFAGWLGGRPALQMFMAGIS
                     LAVSAVPEGLPAVVTITLALGVRIMARQKALLRHLQTAETLGATSVICTDKTGTLTKN
                     EMTIQKLWLASGEIDVSGVGYEPEGEFSKDGQGIDALSNPDLKALLETGRVCNHARIT
                     RADNGWAAIGSPTEAAFIAAAEKAGLEESGHSAVCEFSFNSTRKRMSVIENCEGHMMV
                     HVKGAPETLLMRSNKILIDSKEQDLNNDVRHTIEQAYKAFAQGGLRTLACARKTLPAQ
                     VTPDEETVETDLTFLGVVGIIDPPRSEVKEAIKKTRHAGIRVIMITGDSPDTALAVGR
                     QIGLKVEKAVSGSELEKMDDEEILRLSDQDIIFARTVPEDKFRIVKLLQSRNQLVAMT
                     GDGVNDAPALKQADIGIAMGVRGTDVAKGAADMVLTDDNFASIIDAIQEGRRQYANIR
                     KFVSYLVSSNFGETLAVFVNIMTGAPLILLPTQILWVNLVTDSVTALSLGVEKAEKNI
                     MDEPPRSIDQPILDKISFMMLALFGSYIGFATLGLYHLHLDESYMVANTVAFTGMVAM
                     ANIRVLNFRSFDKPMHVIGWFSNPWLLVAVSSMLGLQLLAVYTPFLQQVLGTVALGWS
                     EWAIIIAFILPMVLFTELYKNLKFIR"
     gene            5093..7702
                     /locus_tag="PK513_05650"
     CDS             5093..7702
                     /locus_tag="PK513_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81967.1"
                     /translation="MSTAREVESYDDPKALRSELKILLKNHRIDGREFGVRLSENADE
                     IIKALTEHHLGNLAQQISFVALGGNGRKDITSQSDLDLLVLLPEALSKSIDTLYEIRD
                     RRTKAGQDPDGKLTLEEQTLFDSSEAKEAAIEFTAGYTYFCADLNDLGFSTASAFPRT
                     LSQTKQETLKDQTIWSSLLDAHLICGDKPLYAELEELLDELRANSNLRAKFAYEKFDE
                     RTKRLEKETRNTFRPDIKNGYGSLRDYQGADWHLKVVFGVKTPKEAEEKGLFSESELK
                     RAQKSYAFLLTMRCHLRNNTANTNDILAPNLRTSLAQQMQYTGVQKMMQDFFMHRREL
                     GFFANITEALVREELEQKQVFEPIQGTHFEISENYIRFSDATITDPVDMLRIYKMAQA
                     QEVNMHHSALRTIRDNVERIAEYRDNPDVNKLFLDILTSDNCTGKALRQMQELGLMQE
                     FLPPFKMLDSMPANDFVHHQTIDEHHFACLEMLNALKKGQHKENAPLATGLFEKGISD
                     SQKRVLYTTVLFYDIGTDQAGGHLAQGAQLAEEYCPRLGLNPEETNMVCWLVENNSFL
                     LQGAVHRDPHDVARIGVFTDCVDTPEKLELLTLLTTARRMSIDPGRWTAYNEKNVLVL
                     YNQALTCLHDQSLTIGPRFELSANHDPGSTNITVVPDHAGGAVITVLTPDKDHLFENV
                     TGALGAQNIRKIVVYTFDKEGDTPVAYQKLSVSSAKPEDGGYKPEDLEKIKVCVEEAI
                     NYDEAHIFPESGYQLRRSWQADKDAIPVNASVAFDNAASDDRTVVDITARDKLGLAHR
                     IASIFGKHGVQVSCVFGESSGGHSITDAFYVSDSRTHKKIIDPDRIADIQKELENLIQ
                     EPVDEYFESLAIG"
     gene            complement(7722..8197)
                     /gene="ssrA"
                     /locus_tag="PK513_05655"
     tmRNA           complement(7722..8197)
                     /gene="ssrA"
                     /locus_tag="PK513_05655"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            8310..9104
                     /locus_tag="PK513_05660"
     CDS             8310..9104
                     /locus_tag="PK513_05660"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOO81968.1"
                     /translation="MKQYLDLMRDVLENGTKKEDRTGTGTYSVFGRQMRFDLSKGFPL
                     VTTKKCHLKSIIHELLWFIAGDTNIKYLKDNGVRIWDEWADENGDLGPVYGKQWRSWI
                     ADNGQKIDQLSNVIEQIKNNPDSRRLLVVAYNPGVVDQMALPPCHAFFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGKFIHTFGDVHLYSNHIEQAKE
                     QLSRKPYPLPEMRINEAVTNIFDFKYEDFELVGYQAHPHIKAPVAV"
     gene            complement(9111..10082)
                     /locus_tag="PK513_05665"
     CDS             complement(9111..10082)
                     /locus_tag="PK513_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81969.1"
                     /translation="MILRGVAVAMTGKNHDVNIMVTSIQMEEGAANDNIEMGERCYDF
                     RLDLCDKGAVIDAFNRVATTFNEVVDMQERHLAKIDEPYRPVALIVGDKQGGNFDYQS
                     LRKTLFNLPDELKLQREDMRDICEKLSQSLRKSVLKIPRFTYHHNDEVSSDERLAALD
                     KENEFLARKKAELDTGEEEALEALVDYALRDGYNYGLNVRKSSSSPDNWCDNWHLRGE
                     LDVRGTQYNDEKGLAPVDSYAGWHPTIKLAFNLAFKRAVGFPANIECKLPYEKRSRFF
                     QDPLRLMVMDQLDVAHGRNSEHNISINYRDFEAGLYCERLEILGSAP"
     gene            complement(10145..10531)
                     /locus_tag="PK513_05670"
     CDS             complement(10145..10531)
                     /locus_tag="PK513_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOO81970.1"
                     /translation="MKKDSHVKIFLDSVEVPLKLGIYEHEQGVSQRVIVDVAVFADAK
                     GYLGGVHVGNIIDYDTYYNEIRSWAGRSQVQLIEDYLKELMALCFRHELVQACRVSIR
                     KLDIYGPDQGAGVEVFMTRTEYESKQ"
     gene            10554..11156
                     /locus_tag="PK513_05675"
     CDS             10554..11156
                     /locus_tag="PK513_05675"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOO81971.1"
                     /translation="MNKSKAPITLSSIVAIARNHVIGKDNGLIWHLPEDLKHFKRTTL
                     GKPILMGRKSYESLGKPLPGRPNIVISRAFQNMPNDNPTPIYKDMEPEEPEAERKINE
                     GPFLYASLEDGIAVMKDMAREMGVDEVFITGGGQIYKETLPYTERLYLTVLDREYEGD
                     TFFPELNWNEWNIAAEEKHDADPVHDRPAFTFYTLEKNRN"
     gene            complement(11275..11350)
                     /locus_tag="PK513_05680"
     tRNA            complement(11275..11350)
                     /locus_tag="PK513_05680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11315..11317),aa:Arg,seq:ccg)
     gene            complement(11361..>12009)
                     /locus_tag="PK513_05685"
     CDS             complement(11361..>12009)
                     /locus_tag="PK513_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO81972.1"
                     /translation="FFTPIQALDLMAGLYGVPKDQRRTMELLRMVGLEDKADAYARSL
                     SGGMKRRLMVAKAMVHNPPILVLDEPTAGVDVELRKQLWGNVRALNVRGVTILLTTHY
                     LEEAEELCDEIAIINHGEVIAHEPTEELLARIENKEIRFRLDRPIDDAERVLSGFCVE
                     RAGERSVVVRYNPSEMQVGVMIEAIQKAGYGIVDISTDDSNLEDVFLQLTGSSGN"
BASE COUNT         3295 a         2881 c         2800 g         3033 t
ORIGIN      
        1 gcaaagaggc cggaacgatt tccgcgctta aatgggcctg catcaccgta gatgacagcg
       61 ctcataattt caggttgggc cagcagcgcg gcttcgaccc attccggtga gatattgcgg
      121 ccgtaagagg taatcagaac gtttttcttg cgcccgttta tatgaagaaa accgtcttca
      181 tccacatagc caagatcgcc ggtggcgaac tctcccttat gcttgttgcc cagataaccc
      241 aaaaaggccg ggtctttaat cgtgatctcc ccatcatttt ccaccaggcg aatatgcggc
      301 aaaatcttac ctgcactgcc cggcttatcg ttttccgggg tattgagtga aacaacggat
      361 gcgcattcgc tcagaccata tccctcgtaa accggcaaac ccatcttacg cgcggcggat
      421 ataagcgaag gcgaaacctt tgccccgccg acagctagga attttaaatg cggcaggtcg
      481 ggccgtacac tcatcagacc acgcaacaac tcgggaacca aaatggccga tgtgatttca
      541 tgcttaccca tataatgcga gagggctgca aaatctggct caaacgggtt ggcaaagccg
      601 gtcattttga gcgaagggag atagacagtt ccgccagcca taagtgtggc ataaacgcct
      661 gcgacattct ccagcaggat ggccagtggc aaaacagaga ggtgtttgtc ggcaagctct
      721 gtgcccagag ctttaacaat ggagcaagca acctgttcta gtcctttttg agaaagacaa
      781 acgccctttg gtgttccggt cgtacctgat gtaaacgtca ccttggccgt gccttctggc
      841 aggaatgcaa ggcccttcag gccggtgtca cgcagttctt tggggaaaag aacatgtgtg
      901 acaccggccg agtgaagagt gtgttgagcc tgttgctcag tgaaaaacgg gggcaaaggc
      961 acacagggaa taccggcttt gagcgccgcc aaatcccaaa gcacccaatc gacactatta
     1021 tccagcgcaa tgcccaaaac cgaaacgcca tagagtgcat ccatgcgctt ttggatttcc
     1081 cgtttcagat cgccatagca caccgtttgc atttcgtcgc acaaggcaat cttttcaggg
     1141 tcatggtttt ggatatgccc gaataacatc agttccggcc ttcctgctta taatgaaggc
     1201 gtgggaacaa gcgcggacgg caatcctcga actctgcacc caacatggct tttaaacgct
     1261 tcacgccggt ctcgacggag ccaaccagca cgcgaggctg tgtgtcataa taactgcccc
     1321 agttttgcgt atcgtgttcc acagccttaa tatcagcatc gcaaatcttg cgcggtttta
     1381 aacccacccg ttcaaaataa cgatgaagaa aatctgtccc ggtgattgcg gcatagcgaa
     1441 tgtctttatt gtttaagtag gaggccagag cagcgaacag aaaggccgac gcgccctgtc
     1501 cggcagaggc cagattgccg atctctacaa tctcattacg cggacaatcc aacagctttt
     1561 ctactgaccc ctgtgtataa tgctctaaaa agagcgattc atctttcgcg tatcggaaac
     1621 cgacagccgc caaaatatcg ccattagcat tacggacact catcagcacc gggtaattta
     1681 cttcgatatc ggcgttatag ctctgcttgt atatcgctct gataaaatcc tcaacacgct
     1741 ggcgctctgg ttcaaacagg ctagtgatgc tgaccaccga gggggtcagc gaactgattc
     1801 ggcgcgtgcg gtcctcacgg agataaagac tggccgtatc aatattattt tcctgataaa
     1861 taagtttgag cacgtcggtg taccttgatt tattgcgttc ttaatcaagg atgccctatg
     1921 aacctgaagt gaagctgaag catatggaga cgattgatgc cccctcttgt gcccccaatt
     1981 tttgaggttc tgtgtgaatg tatatgaaca tgtagaatta tacgtaaaat atgaaatcct
     2041 tgcaaatcaa ggcttttgcg gtcattgata aacgtgagcg aatgttgatg aagagaaaat
     2101 ttggcccgta cggctcaaac atttaaggct actgaacggt tctcgtaaga gatactgcag
     2161 catattcctg acgacgaaca tttaggttca acgccccccg caaaaaaaca gttacaactg
     2221 ctcaaaaaat ggataaagtc cggacagtca acctaaacat ctatgccaac ttcttttttt
     2281 atgacctgtt ttcatgcatt atctgctgaa gagacaatta aatcgttgga aaccagcgct
     2341 aagaacggat tgacggaatc tgaaatccag gtgcgccaaa gtcaatatgg ttcaaacact
     2401 ctgatagaaa aggaatctgt gaaatggccg ctggttctca ttcgtcaatt tacaagtttt
     2461 ctgattttaa tcttattggt tgctgcggcg ttgtcttttt tcataggcga tgagcttgat
     2521 acgctggcta ttttcgccat tgttattctc aacggtcttt tgggctttgc gcaggaatgg
     2581 aaagctgaaa cagcactaaa gagtctgaag aaaatgctca gtccaagatg ccgcgttatc
     2641 cgggattccc gcatgcagga gattgatgcg gctgcgttgg tgcctggcga tatcgtgctt
     2701 ttggagagcg gtgattccgt tcctgcagat attcgtttgc ttgacgaggt caatttaaga
     2761 gcagacgaag cagctttgac cggagagtct gtgtcggtcg ccaaaaatat tcatgcaatc
     2821 agcaaagatt ccccgatcag ctggcgtact tgtatggtgt ggatgggaac gcatatcgtc
     2881 aacgggcgag ggcaaggcgt tatagtggcc acaggtatgg agactgagtt cggccgtatt
     2941 gcgaagctaa ctggcgatat tcaggaaacg cagacacgac tgcagcggca gttggccgtg
     3001 ctggccaagc aattaacggt tgtcattgtc ggtgtaacag cggttgtcat gtttgccggc
     3061 tggcttggag ggaggccagc actgcaaatg tttatggcag ggatttcttt ggccgtttct
     3121 gctgtgccgg aaggcttgcc tgcggttgtg accattactc tggccttagg cgtacgtatt
     3181 atggcgcggc aaaaagctct tttgcggcat ttgcaaacgg cggaaactct tggggcgact
     3241 tctgttattt gcacggataa gacaggcacg ctcaccaaga atgaaatgac aattcaaaaa
     3301 ctgtggctgg ccagtggtga aattgatgtg agcggagttg gttatgagcc cgaaggagaa
     3361 tttagtaaag acggtcaggg aattgatgcc ttgtcgaacc cggatttgaa agccttgctg
     3421 gaaacgggcc gcgtttgtaa ccatgcgcgt attacccgcg ctgataatgg ctgggcggcg
     3481 atcggttccc ccacggaagc ggcctttatt gcagcggcgg aaaaggcagg gctggaggaa
     3541 tctggtcatt ctgcagtttg tgagttttcc tttaattcca cgcgcaaacg catgtccgtg
     3601 attgaaaact gcgaagggca tatgatggtg catgttaagg gcgcgccgga aacattgtta
     3661 atgcgttcga acaaaattct aattgacagc aaagaacagg atttaaacaa tgatgtgcgt
     3721 catacaatcg aacaagcgta taaggctttt gcacagggcg gcctgcgtac gttggcatgc
     3781 gcccgtaaga cacttcctgc acaagttacg ccagatgaag aaacggtgga aaccgatcta
     3841 acatttcttg gggtggtcgg aattattgat ccaccgcgca gcgaagttaa agaagccatt
     3901 aaaaaaacaa ggcatgccgg aattcgtgtg atcatgatta caggggattc acctgatacg
     3961 gccttggctg tggggcggca gatcggtttg aaagtggaga aagccgttag cggttccgaa
     4021 ctggagaaga tggatgatga agaaatattg agactgtcag atcaagatat tatttttgca
     4081 cgcactgtgc cggaggacaa atttcgcatt gttaaactct tgcaatcacg taatcagctt
     4141 gtggcgatga cgggagacgg agtgaatgat gcgccggcac tgaaacaggc agatataggc
     4201 attgccatgg gtgtgcgcgg tactgatgta gccaaggggg ctgcggatat ggtgctaaca
     4261 gatgataatt ttgcatcgat cattgacgct atacaggaag gccgcagaca atatgccaat
     4321 atccgtaaat ttgtcagcta tcttgtgtct tcaaatttcg gcgagacgct tgctgttttt
     4381 gtaaatatta tgactggggc cccgttgatt ttgctaccga cccagattct ttgggtcaat
     4441 ttggtaaccg acagtgtgac ggcgctttct ctcggcgtag aaaaagctga gaaaaatatc
     4501 atggatgaac caccgcgcag cattgatcaa cctatcctcg acaaaatatc ttttatgatg
     4561 ctggctttat ttggctctta tatcggcttt gcaacattgg gcctttatca tttgcatttg
     4621 gatgaatctt atatggtggc caacacggtt gcttttaccg gcatggtggc gatggccaat
     4681 atccgcgttt tgaattttag aagttttgat aaacctatgc atgtgattgg ctggttttcc
     4741 aatccatggc ttctggtagc tgtgtcttca atgcttggat tacaactttt ggccgtttat
     4801 acgccctttt tacaacaggt tttgggcact gtggcgcttg gctggtctga atgggcaata
     4861 attattgctt ttattttgcc catggtactt tttaccgagc tttataagaa tttaaaattt
     4921 attcgttaga aggcgcaaag ctgtgcggta gcactgaact tttaggcgca taaattatgc
     4981 aaaaagtcat tcaatacact caatgtttcc atattgtttt tttattttac aaggcgtgga
     5041 aattgtggca taagcaattt ctaatttatt ccagaaagag gggcgttcat cagtgtctac
     5101 agcacgagaa gttgaaagtt atgatgatcc gaaggcactt cgttcagaat taaaaatcct
     5161 cttaaaaaat caccgaattg atgggcggga atttggtgtg cgcctaagtg agaatgctga
     5221 tgaaatcata aaagcgctga cagaacacca tcttggcaat cttgcgcagc agatttcttt
     5281 cgttgcgtta ggcggaaacg gtcgtaagga tattacctca caatcagatc tcgatctttt
     5341 ggtattgctg ccggaagcat tgtccaaaag tattgatact ctttatgaaa ttcgagatcg
     5401 cagaacaaag gctggtcagg accctgacgg aaaattgaca ctggaggagc agactctttt
     5461 tgatagttct gaagcaaagg aagcagcaat cgagtttacg gcagggtata cttatttctg
     5521 cgccgacctc aatgatttag ggttttcaac ggccagcgct tttccgcgta cactttcaca
     5581 aacaaaacaa gagactttga aagaccagac aatctggtct tctcttctcg atgcgcacct
     5641 tatctgcggc gataaacctc tatatgcaga attggaagag cttctggatg aattaagggc
     5701 gaatagcaat cttcgggcaa aatttgcgta tgaaaaattt gatgaacgca ccaaacgtct
     5761 tgagaaagaa acaagaaata cgtttaggcc tgatataaaa aacggttatg gcagtttgcg
     5821 cgattatcag ggcgcagatt ggcatttaaa agttgtgttt ggggtgaaaa caccaaaaga
     5881 agcggaagag aaaggcctat ttagtgaatc tgagttgaaa agagcgcaaa aatcctatgc
     5941 ttttttgtta acaatgcgtt gtcatttacg gaacaataca gcgaatacaa atgatatttt
     6001 agcaccaaat ttgcgaacca gtttagctca gcaaatgcag tatacgggcg ttcaaaaaat
     6061 gatgcaggat tttttcatgc atcgacgcga attgggtttt tttgcaaata taacggaggc
     6121 tttagttcgc gaagagttag agcaaaaaca ggttttcgaa ccgatacagg gtactcattt
     6181 tgagatttca gaaaactata ttcgtttttc agatgccacg atcacggatc ctgttgacat
     6241 gctgcggatt tacaagatgg cacaggcgca agaggtgaac atgcatcata gcgccttgcg
     6301 cactatccgt gacaacgtag aacgcatagc cgaatatcgg gataatcctg atgttaataa
     6361 gctattttta gatattttaa cgtctgataa ctgtactggt aaggctttgc ggcaaatgca
     6421 ggaactcggc cttatgcaag aatttctgcc gccatttaag atgcttgatt ccatgcccgc
     6481 aaatgacttt gttcatcatc agacaattga tgaacatcac tttgcttgtt tggaaatgtt
     6541 aaatgctttg aagaaaggtc agcataaaga aaacgcaccg cttgctacag gtttattcga
     6601 aaaaggcatt agcgattccc aaaagcgcgt tttgtatacg accgttttat tttatgacat
     6661 tgggacagat caggccggag ggcatttggc gcagggcgcc caacttgctg aggaatattg
     6721 tccgcgcctt ggtttgaacc cggaagaaac gaatatggtc tgctggctgg tagaaaataa
     6781 ttcgtttctt ttgcaaggcg cagttcatcg tgatccacat gatgtggcaa gaattggagt
     6841 tttcacagac tgcgttgaca cgccggaaaa actggaatta ttgacgctct taacaacggc
     6901 tcgcaggatg agcatagatc caggccgttg gaccgcatac aatgagaaga atgtcctcgt
     6961 tctttataat caagcgctta cttgccttca tgatcagagc ttgacgattg ggccgagatt
     7021 tgaactatcc gccaaccatg accccgggag cacgaatata accgtagtgc cagatcatgc
     7081 cggtggcgct gtaattactg ttcttacgcc tgataaagac catctttttg aaaatgttac
     7141 cggagcgctc ggcgcccaga acataagaaa aattgtcgtc tatacttttg ataaagaagg
     7201 agacaccccg gttgcttatc aaaagctttc cgtaagttct gccaagccgg aggatggggg
     7261 atacaagcct gaagaccttg agaagattaa ggtgtgtgtt gaagaggcta ttaactatga
     7321 cgaagcgcat atttttccag aaagcgggta tcaattacgc agatcatggc aagcggacaa
     7381 ggatgctatt cctgttaatg caagtgtagc cttcgataac gctgcaagcg atgatagaac
     7441 agttgttgat attacggctc gcgacaagct gggattggca caccggatag cgagcatttt
     7501 tggtaaacat ggcgtacaag tgagttgcgt ttttggtgag agcagcggcg ggcatagtat
     7561 aaccgatgct ttttatgttt ctgattccag aacgcataaa aaaattattg atcctgatcg
     7621 aatagcagat attcaaaaag aacttgagaa cttgattcaa gagcctgtag atgaatattt
     7681 cgagagtttg gctataggat aaaaaagacg ctgacataga aaatgggccg attctgttgc
     7741 gaggttcgac ccggacccca cctaagacgc attaagcctt agggattagt gcgtagcgca
     7801 ccaaagcggc aattaagcag ctttagtttc accagcaaca tcaccaaagg tgttgtcgtt
     7861 ggctgctaca aatgcatgat tgtcgtttgc atttataacg atcatccggt aacggcggat
     7921 tgccgaacgc cgcatgatgc ctttactacg tgtgtcgatc ctgtttcagc cccatcaaaa
     7981 ttcgttatcg agtgcagcga gttcaaaaat cttggatgtg ctctttgcgg acacgccgaa
     8041 attcaaaacc agttctatac gatcccaaat attggtggag ctgccgggta ctgcccccgg
     8101 gtccacgaac gcctattccg caaccgcgtt tagcgtcata accgggacat gcccggcaac
     8161 taccattata agataaactt taatttttgg aaaggttttt gaaacgccgc gcttgacgag
     8221 tgacgcgcac agaaaagccg aaaatatttt gtgggcggct gtgggtgcgc ccctacactt
     8281 catatacaga atcgtccata ttggtagata tgaaacaata tcttgatctc atgcgcgacg
     8341 tactggaaaa cggcacaaaa aaagaagacc gcaccggcac cggcacctac agcgtttttg
     8401 gccgccagat gcgctttgac ctgtccaaag gctttccact ggtcaccacc aagaaatgcc
     8461 acctcaaatc catcattcac gaactgctct ggttcatcgc cggggatacc aacatcaaat
     8521 acctcaagga taacggtgta cgcatctggg acgaatgggc cgatgaaaac ggggacctgg
     8581 gaccggttta cggcaagcaa tggcgaagct ggatagccga taatggccaa aaaattgacc
     8641 agctttcaaa cgtcatcgag caaatcaaaa acaatcccga cagccgccgc ctactggtcg
     8701 tggcctataa tccgggtgtg gtcgaccaaa tggccctgcc gccctgccac gcctttttcc
     8761 aattctacgt tgccaatggc aaattaagct gccagcttta ccagcgcagc gccgacatct
     8821 tcctcggcgt gccgtttaac atcgcctcat acgcgctgct caccatgatg attgcgcagg
     8881 tcactggtct caaacccggc aaattcatcc acactttcgg cgatgtgcat ctctattcca
     8941 accacatcga acaggccaag gaacaactgt cccggaagcc ctatcccctg ccggaaatgc
     9001 gtataaacga agctgtcacc aacatcttcg atttcaaata cgaagatttc gaactcgtcg
     9061 gctaccaggc ccacccgcac atcaaggccc ccgtggcggt ttagaacgaa ttatggcgcg
     9121 ctgccgagta tctcaagacg ctcacaataa agaccggcct caaaatctct ataattgatc
     9181 gagatattat gctcgctgtt acgaccgtgc gccacatcga gttgatccat aaccattaac
     9241 cgtaaagggt cctgaaaaaa ccttgaacgc ttttcgtaag gcaacttaca ttctatattc
     9301 gccggaaaac caacagctct tttaaaagcc agattaaatg ccagcttgat tgtaggatgc
     9361 caccccgcat aagagtctac aggagcaaga cctttttcat cattatactg agtgcctctt
     9421 acgtcgagct cacctcgaag atgccagttg tcgcaccagt tgtccggaga tgaagatgac
     9481 ttcctaacgt tgagcccgta attataacca tcccttaacg cataatccac aagcgcctca
     9541 agagcctcct cctcacccgt gtccaactct gccttttttc gcgctaaaaa ttcgttctct
     9601 ttatccaacg cagcgaggcg ctcatctgat gaaacctcat cattatgatg gtaggtaaag
     9661 cgaggaattt tcaaaacaga ctttcttaag gattgtgaaa gtttttcgca aatatcacgc
     9721 atatcctcac gctgtaactt taattcgtcc ggcaaattaa acaaagtctt gcgcaaagat
     9781 tgatagtcga aattccctcc ctgcttatct ccaacaatca gggccacggg ccggtaaggc
     9841 tcatcgatct ttgctaaatg ccgctcctgc atatcgacaa cttcattaaa cgtcgttgca
     9901 acccgattga atgcgtcaat aactgcgcct ttatcacaaa gatcaaggcg aaaatcataa
     9961 caacgctcac ccatttcgat gttatcattc gcagcccctt cttccatctg aatagatgtc
    10021 accatgatgt ttacatcatg gttttttccc gtcatagcaa ctgcgacacc ccgtaaaatc
    10081 atacaataat cctcttaagc tcgaacaaaa aggaaacata tagaagttaa aataatatgt
    10141 caaattattg tttgctttca tactccgtgc gggtcataaa cacctccacg cccgcgcctt
    10201 gatccggccc ataaatatca agcttgcgaa tactcacccg gcaggcctgc accaattcat
    10261 gacgaaaaca cagcgccatc aactctttca gataatcttc gatcaactgc acctgcgaac
    10321 gcccggccca tgaacggatc tcattataat aagtgtcata atcaataata ttgcccacat
    10381 gcaccccacc cagatagccc ttcgcatcgg caaacaccgc cacatcaacg ataacgcgct
    10441 gggacacgcc ctgttcatgc tcgtaaatac caagcttgag cggcacctca acggaatcaa
    10501 gaaaaatttt aacgtggctg tcttttttca tgatttcgct ttatatacaa agaatgaata
    10561 agagcaaagc ccccataact ttaagttcaa tcgttgctat cgccagaaat catgtgatcg
    10621 gcaaggataa cggcctgatc tggcacctac ccgaagacct caaacatttc aagcgcacga
    10681 cactgggcaa gccgatcctg atgggccgca aatcttacga aagcctcggc aaaccgctcc
    10741 ccggccgccc caatatcgtt atcagccgcg cctttcaaaa catgcccaac gacaacccga
    10801 ccccgattta caaggatatg gaacccgaag aacctgaggc cgagcgcaaa atcaatgaag
    10861 gcccgttcct ctatgcttcg ctggaagatg gcatcgccgt gatgaaagac atggcccgtg
    10921 aaatgggcgt cgatgaagtt ttcatcaccg gcggcgggca gatttataaa gaaaccctac
    10981 cctacaccga acgcctctac ctcactgtgc tggaccgcga atatgaaggc gatacctttt
    11041 tcccagagct aaactggaat gaatggaata tcgccgcaga agaaaaacac gatgccgacc
    11101 cagtccacga ccgcccggcc tttacatttt atacattgga gaaaaacagg aactaacacc
    11161 tacatatagc ctttgccgac cgtcttgccc atgtccgaaa tgaagggttt agaaccacaa
    11221 attttgcatg atatttcaag ttgttagccg gatttggagt aaatgatatc gaaatggcgc
    11281 gctcggcagg attcgaacct gcgactcctg gctccggaaa ccagtgctct atcccctgag
    11341 ctacgagcgc ttaggcgtta tcagttacca gaactgccag tcaactgcaa gaacacatct
    11401 tccaaattac tgtcgtcggt cgaaatatcg acaatgccgt atcccgcctt ttgaatggcc
    11461 tcaatcatca ccccgacctg catttccgac gggttatagc gcacaacaac gctgcgttct
    11521 cccgctcttt cgacacaaaa cccagacaaa acccgttccg catcatcgat aggacgatca
    11581 agccggaacc ggatttcttt attctcaatc cgcgccagca actcttccgt cggctcatgc
    11641 gcaatcacct cgccatgatt gataatcgcg atttcatcac ataattcctc cgcctcttcc
    11701 agataatgcg ttgtcagcaa aatcgttaca ccccgcacat tcagggcccg cacattaccc
    11761 caaagctgct tacgaagctc tacatccact ccggctgtcg gctcatcaag caccaaaatc
    11821 ggcggattat gtaccatcgc cttggccacc atgagacgcc gtttcatccc gccagaaagg
    11881 ctgcgcgcat acgcatcagc cttatcttcc aaacccacca tcctaagcag ctccatcgtc
    11941 cggcgctgat ccttcggcac accatataag cctgccatca aatccagcgc ttgtatcggc
    12001 gtgaaaaac
//
LOCUS       DAOSWR010000058        11967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3386_length_11967_cov_5.473388, whole genome shotgun sequence.
ACCESSION   DAOSWR010000058 DAOSWR010000000
VERSION     DAOSWR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_11967_cov_5.473388"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PK513_05690"
     CDS             complement(<1..1104)
                     /locus_tag="PK513_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017989438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOO81973.1"
                     /translation="METFSALAYIASAMLFILALRGLSHPESARQGLTFGMIGMALAI
                     VTTLLLPQVGGTFWILIGIGIGGAVGAHIARTIDMTNLPQLMAGFHSLVGLAAVCVAA
                     AAFYTPEAYGIGTKNDIHFASMVEMSLGLAIGAITFTGSIIAWAKLEGRMSGKPITFG
                     GQHALNGMLGALLLLLILILCTTESSFIFWLVALLSMALGVLMIIPIGGADMPVIVSM
                     LNSYSGWAAAGIGFTLHNNLLIITGALVGASGAILSYIMCKGMNRSFMNVILGGFGGE
                     QSASTEHDHGDRNAKIGSAEDAAYIMKNASKVIIVPGYGMAVAQAQHALREMADILKK
                     EGVDVKYAIHPVAGRMPGHMNVLLAEANVPYDEV"
     gene            complement(1108..1521)
                     /locus_tag="PK513_05695"
     CDS             complement(1108..1521)
                     /locus_tag="PK513_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO81974.1"
                     /translation="MPDTQRTPSESLKELIEQGNAVKDQIEQATIQAAEAVQADMVHG
                     QPFLITGLTVFILACFVGYHVVWRVTPALHSPLMGITNAISSVIIVGAIMAVWPKEEG
                     FSLLGFLAVIFASVNIFGGFIITRRMLSMFKKKGS"
     gene            complement(1521..2783)
                     /locus_tag="PK513_05700"
     CDS             complement(1521..2783)
                     /locus_tag="PK513_05700"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOO81975.1"
                     /translation="MGLKIAIPKETAANEKRVAASAETVKKLKDMGALVMIESGAGEA
                     ASITDETYKASGGTISKGLEDLYKNADIVLKVQAPNENELKSIPKGALLIALLAPYSN
                     ADLVSNLARAGINAFAMELVPRISRAQSMDVLSSQSNLAGYKAVLDATEHYARAFPMM
                     MTAAGTIPPAKVFVMGAGVAGLQAIATARRLGAVVSATDVRPAAKEQVESLGASFVAV
                     ENEEFKEAETAGGYAKEMSKDYQKRQAELVAGHIAKQDIVITTALIPGRPAPQLIDEP
                     SVKSMKPGSVIVDLAVEAGGNCAGSSVGKVVQKHGVSIVGHANVPSRLAADASALYAK
                     NLFNLLKLIIDSETSKLAIDWDDEIIKGIALSRDGEIIHPNFAVKKAEAKTAKKQASE
                     EKPSEKKTKPAAKKAPAKKTSANKKEKK"
     gene            3082..4413
                     /locus_tag="PK513_05705"
     CDS             3082..4413
                     /locus_tag="PK513_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO81976.1"
                     /translation="MRLKFLSLFSGVCLVGVPCTSFAETLQQAVSITLSQHPQVESAQ
                     AAFGRSQQEQKEEFSAYFPELNVTATGGRIYGDNSTSRGLSVTRGTGYSYLWEGSVSA
                     RQKIFDGMETRNRVGAARSQVKAAELALVDVRENLAYQTAQTYLNLLRVQKGLALLNG
                     QQKTVAEYLQRIRTMVEDGAADEAELQQARDVSIILDNFIADYEGQARALESDYLELT
                     GHLPESALTNPELAVDQIPQDLENALIFAKEKHPLLRAAQFRSRSAEQGMEAEKSTYV
                     PKFDGELSFLKSDKADVIGGEVEDGRAVVRMNWGFETGGAQKARIKQRTYDYKESQAR
                     ITEIERQVERAIRQAYAEQETAQRQLENQKRRQDLNKKLLETYEVQFEGARISVLQLM
                     QADNQALLTKLETENAKSRVLIAQYGILAAMGRLQEAMNIQLAASHPRHEK"
     gene            4453..6609
                     /locus_tag="PK513_05710"
     CDS             4453..6609
                     /locus_tag="PK513_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOO81977.1"
                     /translation="MTKNAPDKKDKKKKPSQKTDDLLECLVFLTAHYGRAKSARALTS
                     GLAYNGHSMHPDLFTEAAQRLGLRTQVVKKAKITDISEAVLPAVLILQGKRACVLLSG
                     SRTPEIYDPGTGRKKQVKLRELQQDYAGYCILTQALPEFTNPYAAASEEDHRHWFWSL
                     INQNKGIFAMVLLGAVFINLFGLASPLFIMNVYDRVIPNNAIETGWALGIGALVVFVF
                     DLIMRSLRAYLLDLAGRRVDVIATRRIYDQVLNMRLCERPKSSGAFANMLRDFDSVRD
                     FFTSATITALVDLPFTLFFLFIIYKLGGIIAFILLGLLGAVSVIGLILQVPLKALVRK
                     AARSAEAKHGMLVETIHGLETIKAIGADGAFRARYGALVGENAAYGQSSRMVSGLGIN
                     AATFIQQIASVVIVLSGMYLVADGALTVGGLIACVILSGRALAPVGQIANLLTRYHQA
                     SGALATLDGIMNKPVERPASRNFLHRPDIKGKITFDQVGFEYPGVKRAVLDKVSFTIN
                     PGERVAIIGRIGSGKSTTARLMMGLYEPTAGHILADDTDYQQIDPADLRRSLAYIAQD
                     VVLFTGSVRDNIAASCPSASEEDILAAAKAAGVHEFVSRHPMGYDAPVGEHGEGLSGG
                     QRQAIALARAMLIKPKVLICDEPTNAMDMQAEVAFKSYVENEIEGKTFILITHKHTML
                     EMVDRLILLDQGRLIMDGPREEVVAALQSGKVQVQG"
     gene            6606..7934
                     /locus_tag="PK513_05715"
     CDS             6606..7934
                     /locus_tag="PK513_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOO81978.1"
                     /translation="MSLFEPKIKETDFMSELDAATKMRPATSATLMLFSIMALVVFGI
                     VWAGLTHVEVLTRGQGQVVPSQDVQIVQSLEGGIVEALLVGPGDLVSKGQVLMRLSDV
                     QFSSEARGTEARFLGLEAKKARLTAEASGEDFKVPPKVAGKAPQVAENEKALYESRQK
                     ELQNAYAILDDRIAKANADMAEVNAQINRFSSSRKLLNDELAITRDLVKKRAVPKLDE
                     IRLNREIADISGQINAQSQRRKSLQAELAVAKKERESQLDKFRSQALGELGAVETEIS
                     SLRESLKSIGDRVDRREVKAPVDGIVNNIAVKTLGGVVEPAMQLVEIVPVDEELKIIA
                     RIQPNEIAFIRPGQPAKVKITAYDPQKYGALEGELVRIGATSGRDREGGTFFEIEVRT
                     DKNYLGSAKNPLPITPGMVADVEVITGKRTILEYLLKPVLRARQRAFTEQ"
     gene            7941..10187
                     /locus_tag="PK513_05720"
     CDS             7941..10187
                     /locus_tag="PK513_05720"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOO81979.1"
                     /translation="MGRLLTHRWIHFILLFVLLLGAVYYSGSQARWRKEMQALVFDEL
                     NTMYPREESGQVLIIDIDDDSLMKIGQWPWPRTYVADLVTNLTTLGAKAIAFDGVLAE
                     ADRSSPRFVAQNLPREERFKALEEEIRRLPDHDDILAKAIKDSRIFISAFTYATYSQT
                     PRKPRLSKQILVKKTQEQEFLKHSEGFEKAAVFLPNLEKASAGNGSFMASPDFDGVLR
                     RTGMIFNDGKKLYPSLSLETLRVAHDPKSYIRIEENPDYKIKKINTRYLITVGDHIIP
                     VERDGILRPYYRRFDENGKDYLSAYKVIEPNYHDEIRKRLQGKYILIGSSAEGLKDLR
                     TTALETFQPGVEIHANIIEQILQGQYLLRPDIMTGVEAIFIFIVGLMMIALVPFVNAI
                     LLGLVCISLIALAFFGATVAYVDYKILLDPFYPSLSVFVIFMVSALLTYLRVENEKHQ
                     VRNAFGMYVSRDVMRDLEKNPDKLKLGGENKDLTVMFTDIRQFTTISEGLEPEELIHL
                     MNEFLTAMSDIVMEHQGTIDKYMGDAMMAFWNAPKDVETHERAACLAALKMQAALEPI
                     NESLSEKAKEDAKEPVLLQAGIGVNTGQCAVGNMGSRQRFAYSTLGDAVNLASRLEGQ
                     TKNYGVQTLIGEDTCAHVPDLATLEMDLIQVKGKTQPVRVYALLGDERMATSSEFRAL
                     KQQHIEMITAYQAGDFKKAQAMLKTCQNLDIYSLSVAYDLYKQRIKDLLKNPPKQEWT
                     GVFEAKTK"
     gene            complement(10206..11423)
                     /locus_tag="PK513_05725"
     CDS             complement(10206..11423)
                     /locus_tag="PK513_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO81980.1"
                     /translation="MHKDEHKKSFQSGEVIMRQGDAGKCAYIIKTGRVEILIETRNGD
                     TQYVGSRGPGAMIGEMAILDDAPRTATVRATEDCELLEITKEDFALRLENADPILHMM
                     TKVILARYRDMLQRSEITGEPPKLNAAEDLELTYAEGSSAVETIKITNDFESALKRHE
                     ISLYYQPVIMLDSGKIAGFEALMRWEHPEKGFISPAVFIPIIEDSGLIVKASAWALKE
                     SLMALSRIENRAGYDHELFMSVNFSGRDFAADDFVDSVYETISLSDVSASQVHLEITE
                     RLLMGQPDNAKETLSMCQKAGMSISIDDFGTGYSSLSYLHYFPIDTLKIDRSFVKDML
                     ANNGSAALVKAIIGLGKSLNMDIIAEGVESQEEAVALRDLGCDKAQGYYFAKPMPEKE
                     IVDFIIKNRKIEF"
     gene            11588..11713
                     /gene="ykgO"
                     /locus_tag="PK513_05730"
     CDS             11588..11713
                     /gene="ykgO"
                     /locus_tag="PK513_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOO81981.1"
                     /translation="MKVTNSLKTLKTRDRNNRVIRRRGRTYVINKKNPRMKARQG"
     gene            11848..>11967
                     /locus_tag="PK513_05735"
     CDS             11848..>11967
                     /locus_tag="PK513_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO81982.1"
                     /translation="MFSGLFGFMSADMAIDLGTANTLVYVKDQGIVLNEPSVVA"
BASE COUNT         3020 a         3034 c         2982 g         2931 t
ORIGIN      
        1 cacttcgtcg taaggcacgt tggcttcggc cagcagcaca ttcatatgcc caggcatacg
       61 ccccgccacc gggtgaatcg catatttgac atccacccct tcttttttaa ggatatccgc
      121 catttctcgc aaagcgtgct gagcctgcgc cactgccatg ccgtagccgg gaacgataat
      181 aactttcgag gcatttttca tgatataggc cgcgtcctca gctgagccga ttttggcatt
      241 acggtcccca tgatcgtgct cggtactggc cgactgctca ccgccaaacc caccaagaat
      301 aacgttcatg aaactccggt tcataccctt gcacataatg taggacagaa tcgccccgga
      361 tgccccgacc agtgcgccgg tgataatcag caaattgtta tgcagcgtaa agccgatccc
      421 ggccgccgcc cagccggaat aggagttcag catcgacacg atcacgggca tatccgcccc
      481 gccgatcggg ataatcatca aaacgcccaa agccatagac agcagggcca ccagccagaa
      541 tataaagctg ctctcagtgg tgcacaggat aaggatgagt agcaaaagca gcgcgcccaa
      601 catgccgttc agtgcgtgct gcccgccgaa tgtaatcggt ttgcctgaca tgcgcccctc
      661 cagcttcgcc caggcaatga tcgagccggt aaaggtaatc gccccgatcg ccagccccaa
      721 agacatttca accatgctcg caaaatgaat atcgttcttt gtgccgatcc cataggcttc
      781 tggtgtgtaa aaggccgccg ccgccacgca caccgcagcc aatcccacga gtgaatgaaa
      841 gccggccata agttgcggta aattggtcat gtcgatcgtg cgggcaatat gcgcgccaac
      901 agcgccgcca atgccgattc ctatcaaaat ccagaatgtg ccgccaactt gcggcaataa
      961 caatgtcgtg acaatcgcca gcgccatgcc gatcatgcca aaagttaagc cctgacgcgc
     1021 gctttcagga tgcgacaacc cgcgcagagc caggataaac agcatggcag aagcgatata
     1081 ggcaagagct gaaaaggttt ccatcgctta tgatcccttc ttcttaaaca tcgacagcat
     1141 ccggcgcgta ataataaaac cgccgaaaat attcaccgaa gcaaagatca cggccagaaa
     1201 ccccagcagc gaaaagcctt cttccttggg ccagacggcc ataatcgccc cgacaataat
     1261 caccgaagaa atcgcattcg taatccccat caatggtgag tgaagggcag gcgttacccg
     1321 ccacacaaca tgatagccga cgaaacatgc cagaataaaa accgtcaggc ctgtaatcaa
     1381 aaacggctgg ccgtgaacca tgtcagcctg cacagcttca gccgcctgaa tcgttgcctg
     1441 ttcaatctgg tccttaaccg cattgccctg ttctataagc tctttaaggc tttcgcttgg
     1501 tgtgcgttga gtatcaggca ttattttttc tcctttttgt tggcggaggt tttcttcgca
     1561 ggagcttttt tagccgctgg ctttgttttc ttttcggatg gcttttcttc agaggcctgc
     1621 tttttagctg ttttggcttc agccttttta accgcaaaat taggatgaat aatctcgcca
     1681 tctctgctca gggcgattcc cttgataatt tcatcgtccc aatcaatcgc aagcttggaa
     1741 gtttcagaat caataatcag ttttaaaaga ttgaacaaat tcttggcata taaagcagag
     1801 gcatctgccg ccaatcggct cggtacgttt gcgtgaccaa caatagaaac accgtgtttc
     1861 tgcaccactt tgcccacgct tgaaccggcg cagttgccgc cggcctcaac ggctaaatcc
     1921 acaatgacgc tgccgggctt catcgattta acggaaggct catcaatgag ttgtggggca
     1981 gggcgcccag gaataagcgc tgttgtaatc acaatatcct gcttggcaat atgcccggca
     2041 accagttcgg cctgccgctt ctgatagtct ttcgacattt ccttggcgta accaccggca
     2101 gtttcagcct ccttaaactc ttcattttcc acagcgacaa aagacgcacc gagtgattca
     2161 acctgctcct tcgccgccgg gcgcacgtcc gtagcgctga caaccgcgcc aagcctgcgc
     2221 gcagttgcaa tggcctgtaa gcccgcaaca cctgcaccca tcacaaaaac tttggctgga
     2281 ggaatagtgc ctgccgctgt catcatcatc ggaaatgccc gcgcataatg ctcagtagca
     2341 tccaaaacgg ccttataccc ggccagattg gactgggatg acaaaacatc catgctctgc
     2401 gcgcgtgata tgcgcggcac cagttccatc gcaaaggcgt taatcccggc tctggctaaa
     2461 ttggagacaa gatcggcatt gctgtacggc gcaagtaaag caatgaggag tgcccctttg
     2521 ggaatgcttt taagttcgtt ttcattaggg gcctgtacct tgagaacaat atcggcattc
     2581 ttgtataaat cttccaatcc ttttgagatt gtgccgccac tggctttata agtctcatcc
     2641 gtgatagacg cggcttcgcc agcaccgctt tctatcataa caagtgcgcc catatctttg
     2701 agctttttaa cagtctcagc agaagccgca acgcgttttt catttgcagc cgtttccttt
     2761 ggtatggcga tttttaatcc cacggtaatt agtccttgtt aagtttaaga gcagttacat
     2821 gcacttagag tttctacgga gatgaggtaa aagatgcccg atctttatag ctttgtgaag
     2881 gggcaaaaca aaatatatcc agaccaattc aagccttatt ttagatctgc gctggcacaa
     2941 cataatacac gctatagatg ttcatgtgcc ttagcccttg taaaacggtg cgtttttatt
     3001 tagattctaa tatagcgatg ggcgttcgcc tccgcttcat taaactgctc tggtttatgt
     3061 ctaacgtgcg ttgtttttaa tatgcgttta aaatttttat cgcttttcag tggagtatgc
     3121 cttgtgggtg tgccgtgcac ctctttcgca gaaacactcc aacaggccgt gtccatcaca
     3181 ctttcgcagc atccgcaggt ggaaagcgcg caggctgctt ttggccgttc ccagcaagag
     3241 caaaaggaag aattctccgc ttattttcct gagctaaacg ttacagccac aggtggacgg
     3301 atttatggtg acaatagtac ctctcggggt ttaagcgtca cgcgaggtac gggttattct
     3361 tacttgtggg aaggctctgt ttctgcgcgc caaaagattt ttgacggtat ggaaacccgt
     3421 aaccgcgttg gcgcggcgcg ttctcaggtc aaagccgctg aactggcgct ggttgatgtg
     3481 cgtgaaaatc tggcctatca aacggcgcaa acatatctaa acctgctgcg tgtacaaaaa
     3541 ggactcgcgt tgctcaatgg tcagcaaaaa accgttgccg aatatctaca gcgcatccgc
     3601 acgatggttg aggatggcgc tgccgatgag gcggaactgc agcaggcgcg cgatgtctct
     3661 atcatccttg ataattttat tgccgattat gaaggacagg ccagagcgct cgaatctgat
     3721 tatttggaac tgaccggtca tttgccggaa agcgcattga cgaatcctga acttgccgtt
     3781 gatcagattc cgcaagacct tgaaaacgcc ttgatctttg caaaagaaaa acatccactg
     3841 ttgcgcgcgg cgcagttccg ctcacgctcg gccgagcagg gcatggaggc ggaaaaaagc
     3901 acctatgtcc caaaatttga cggggagctc tcgtttctga aaagtgataa agccgatgtg
     3961 ataggcggtg aagtcgaaga cgggcgagcc gttgtacgca tgaattgggg gtttgagacc
     4021 ggaggcgccc aaaaggcgcg catcaagcag cgcacctacg attacaaaga atcacaggca
     4081 cgtatcacgg agattgagcg tcaggttgaa cgcgcaattc gtcaggccta tgcagagcaa
     4141 gagactgcac agcgccagct tgaaaaccag aaacggcgcc aggacctgaa caaaaaactc
     4201 cttgaaacct atgaggtgca gttcgaaggc gcgcgtattt cggtgctgca attgatgcag
     4261 gctgacaatc aggcgctgct caccaagctg gaaacagaaa acgcgaaaag ccgcgttttg
     4321 attgcacaat atggtatatt ggcggcgatg gggcgcttgc aagaggcgat gaatattcag
     4381 cttgccgctt cgcatccgcg ccatgaaaaa taaagtctaa aataaggggc cgtctggctt
     4441 ttttaagaat ccatgacaaa aaatgcgcca gataaaaaag ataaaaaaaa gaagccgtcg
     4501 caaaaaacag acgacctctt agagtgtttg gtttttttga cagcgcatta tggccgcgcc
     4561 aaatccgccc gcgcattgac ctcggggctg gcctacaacg ggcactctat gcaccctgac
     4621 cttttcactg aagcggcgca gcggttaggc ttgcgcacac aagtggtaaa aaaagcgaaa
     4681 atcaccgata tatccgaagc tgtactgcct gcagtattaa ttttgcaagg caagcgtgcc
     4741 tgtgtgctgc tttccggttc cagaacaccg gaaatctacg accctggtac ggggaggaaa
     4801 aaacaggtca aactgcgcga gcttcagcaa gactacgccg ggtattgcat cctcactcag
     4861 gcactgcccg aattcacaaa tccttatgcc gccgcctctg aagaggatca ccggcactgg
     4921 ttctggtcac tgatcaatca gaacaaaggc atttttgcta tggtgctgct tggcgcggtt
     4981 tttattaatc tgttcggtct ggctagccca ctctttatta tgaatgtgta tgatcgcgtt
     5041 atcccgaaca atgccatcga aaccggttgg gctctgggca ttggtgcgtt ggttgtgttc
     5101 gtatttgatt tgattatgcg ctcccttcgt gcgtatctcc tcgatctggc cggtcgccgg
     5161 gtggatgtta tcgccacgcg ccggatttac gatcaggtcc tgaatatgcg tttgtgcgag
     5221 cggcctaaat ccagcggcgc gtttgctaat atgttgcgcg attttgattc ggtccgcgat
     5281 ttctttactt ctgccaccat caccgcgctg gtcgatttgc ctttcacgtt gttcttcttg
     5341 ttcattattt acaagctcgg cggcatcatc gcttttattt tgctcggtct gctgggcgct
     5401 gtatcagtga ttggtctcat tctgcaagtc ccgttaaaag cactggtacg caaggctgcg
     5461 cgatctgccg aagccaagca cggtatgctt gttgaaacca ttcacgggct ggaaacgatt
     5521 aaggccatcg gcgccgacgg agcgttccgt gcacgctacg gtgctctggt tggagaaaac
     5581 gcagcttacg gtcaatcttc gcgtatggtt tctggcttgg ggattaacgc ggccaccttt
     5641 attcagcaaa tcgcctctgt ggtgattgtg ctgagtggga tgtacttggt cgccgatgga
     5701 gcgctgaccg ttggcggact gattgcctgc gtcattcttt cgggccgcgc tcttgcccct
     5761 gtcggccaga ttgccaacct gctcacgcgt taccatcaag ccagcggcgc tttggcgacg
     5821 ctcgatggca ttatgaacaa gccggtggaa cgtcctgcaa gccgcaactt tctgcatcgc
     5881 cctgacatta aaggaaagat cactttcgat caggttggtt ttgaatatcc cggagttaaa
     5941 cgcgccgtgc tcgataaggt cagcttcacc atcaatcccg gcgagcgcgt ggcaattatt
     6001 ggccgcatcg gttctggcaa aagcacaacc gcgcggctaa tgatggggct ctacgaacca
     6061 accgccgggc acattctcgc cgatgatact gattaccagc agatcgaccc ggcggatctg
     6121 cggcgctctt tggcctatat cgctcaggat gtcgtgcttt ttaccggctc tgtgcgcgat
     6181 aatattgctg ccagctgccc ttcggccagt gaagaggaca ttcttgccgc cgccaaggcc
     6241 gcaggtgtgc atgaattcgt ctcccgccat ccgatgggct acgatgcgcc tgtcggtgaa
     6301 catggcgaag ggctttctgg tggacagcgc caggccatcg cactggcccg cgccatgctg
     6361 atcaaaccta aagtcctgat ttgcgatgag ccgacaaacg ctatggatat gcaggccgag
     6421 gtcgcattca aatcctatgt tgaaaatgaa attgagggca aaacctttat cctgatcacc
     6481 cataaacaca caatgttaga aatggttgat cgcctgattt tgctcgatca ggggcggctg
     6541 attatggacg gtccgcgtga agaggtcgtc gccgcgctgc aatctggaaa agtgcaggtg
     6601 caaggatgag cctgtttgaa ccgaaaatca aagaaaccga tttcatgtcc gaactcgacg
     6661 cggccacgaa aatgcgccct gcgacttcag cgacattaat gttgttttcc attatggcgc
     6721 tggttgtgtt cggaattgtc tgggccgggc ttacccatgt tgaggttttg acccgcgggc
     6781 aggggcaggt ggttccatct caggatgttc agatcgttca aagtcttgaa ggtgggattg
     6841 ttgaagcgtt gttagtaggg cctggcgatc ttgtaagcaa aggacaagtg ctcatgcgtc
     6901 tgagcgacgt gcagttctca tccgaagcac gcggcaccga agcccggttc ttgggactgg
     6961 aggctaagaa ggcgcgcctc actgctgagg ccagtggtga agattttaaa gtgccgccaa
     7021 aggtcgcggg aaaagcgccg caagtcgcag agaatgaaaa agcgctctac gagtccagac
     7081 aaaaagaact gcaaaatgcc tacgcgattc tggatgaccg catcgccaag gccaatgctg
     7141 atatggcgga agtaaacgcg cagatcaatc gcttttcctc cagccgtaaa ttgctcaatg
     7201 atgaacttgc catcacgcgc gatctggtca aaaaacgcgc cgtgccaaag ctcgacgaaa
     7261 ttcgcctgaa ccgcgaaatt gccgatataa gcggccagat caacgcgcaa agtcaacgcc
     7321 gcaaatcctt gcaggctgaa ctggccgtgg cgaaaaaaga acgcgaaagc cagcttgata
     7381 agtttcgttc gcaggcttta ggcgaactgg gcgccgttga aaccgaaatt tcctcactgc
     7441 gcgaaagtct caaatccatt ggcgatcgcg ttgataggcg cgaggttaaa gcccctgttg
     7501 atggcattgt caataatatc gccgtcaaaa cgctcggcgg cgtggtcgaa ccggcaatgc
     7561 aattggtcga aatcgtaccc gttgatgaag agcttaaaat catcgcccgc atccagccca
     7621 atgaaatcgc cttcattcgc cccggccagc ccgccaaggt taaaatcacg gcctatgatc
     7681 cacaaaaata tggggcacta gaaggagagt tagtgcgtat tggcgcgacc tcaggccgtg
     7741 atcgggaggg gggaacgttt ttcgaaatcg aagtgcgcac tgacaagaac tatcttggca
     7801 gcgcaaaaaa tccattgccg ataacgcctg gtatggtcgc cgatgtcgag gtgatcaccg
     7861 gcaagcgtac gatccttgaa tatcttctta agcccgttct gcgcgcccgc cagcgcgcct
     7921 ttacggagca atagagcacg atgggtcgtc ttctcacaca tcgctggata cactttatat
     7981 tactttttgt cctcctgctt ggcgcggtct attacagcgg ctcacaagcc cgctggcgca
     8041 aggaaatgca ggcactggtt tttgatgagt tgaacaccat gtatccgcgc gaagaaagcg
     8101 ggcaggtgct gatcattgat atcgacgacg attccctgat gaaaatcggc caatggccgt
     8161 ggccacgtac ctatgttgca gatttagtta cgaaccttac gacattagga gcaaaggcca
     8221 tcgcttttga tggtgtgctg gccgaagccg atcgctcttc accgcgtttt gttgcgcaga
     8281 acttgccgcg cgaagaacgt tttaaggctc ttgaagaaga gatcaggaga ctgcccgatc
     8341 atgacgatat tctggctaag gctataaagg actccagaat ttttatatct gcttttacct
     8401 atgcaaccta ttctcagacc ccccgtaaac ccagattatc caaacagata ttggtaaaaa
     8461 aaacgcaaga gcaagaattt ctaaaacact cggaaggttt tgaaaaagct gcagtatttt
     8521 tgccaaacct cgaaaaagct tctgccggga acggcagctt tatggccagc ccggattttg
     8581 atggagtttt gcgccgcacg ggtatgattt ttaacgatgg aaaaaaactg tatccgtctt
     8641 taagcctcga gactttgcgt gtcgctcacg accccaaatc ctatatcagg attgaagaaa
     8701 atcccgatta caaaatcaaa aagatcaata cgcgctatct tataaccgtc ggcgatcaca
     8761 tcattcctgt tgaaagagac ggaattcttc gcccctatta ccgccgtttt gacgaaaatg
     8821 gcaaggacta tttatcggca tataaggtca tagaaccaaa ctatcacgac gagatcagaa
     8881 aaaggctgca gggcaaatat attctcattg gctccagcgc tgagggtctc aaggatcttc
     8941 gcacgacagc gttggaaaca tttcagcccg gcgtagaaat acacgccaat attatcgagc
     9001 agattcttca agggcagtat cttctgcgcc cggatattat gaccggagtt gaggcgatct
     9061 ttattttcat agtcggattg atgatgattg cgctggtgcc ttttgtgaac gcgatacttc
     9121 tagggctggt ttgtataagc ttgatcgcgt tggcattttt tggagccact gtggcttatg
     9181 ttgattacaa aatcctgctc gatccctttt accccagcct gtccgtgttc gtgattttta
     9241 tggtgtcggc gcttctgact tacctgcgcg tggaaaacga aaagcatcag gtgcgcaacg
     9301 ccttcggtat gtacgtctcg cgagacgtga tgcgcgatct agaaaaaaat cccgataagc
     9361 taaagcttgg tggggagaat aaagacctca cggtgatgtt tacggatatt cgccagttca
     9421 ccaccatttc agaagggctg gaaccagagg aacttattca tctcatgaat gagtttttaa
     9481 cagcgatgag cgacatcgtc atggaacatc aaggtacgat cgacaaatac atgggcgacg
     9541 ccatgatggc gttttggaac gcgcctaaag atgtagaaac gcatgaacgc gccgcctgcc
     9601 tggctgcgtt gaaaatgcaa gcagctctgg aacccatcaa tgagagcttg agtgagaaag
     9661 caaaggaaga cgctaaggag ccggttttat tgcaagcagg aatcggtgta aataccggcc
     9721 agtgtgcggt cgggaatatg ggctcacgcc agcgctttgc ctattccacg ctgggcgatg
     9781 ccgtcaattt ggcatcgcgc cttgaaggtc agaccaagaa ttacggcgtg cagactttaa
     9841 ttggcgaaga tacctgcgcg catgtgccgg atcttgcaac gctggagatg gacctgattc
     9901 aagtcaaagg aaaaacgcag cccgtgcgtg tatacgcgtt gctcggcgat gagcggatgg
     9961 caacaagttc tgagtttagg gcattgaaac agcaacatat tgagatgatt acagcctatc
    10021 aggctggaga ttttaaaaag gcgcaggcta tgctcaaaac ctgccaaaat ttggatattt
    10081 atagtttatc tgtcgcctat gatctttata agcagcggat taaagatctt ctaaagaatc
    10141 cgccaaagca agagtggacc ggtgtttttg aagccaagac aaaataaagt ccgcaatcaa
    10201 cggatctaaa attctatttt tcgattcttg ataatgaaat ctacaatctc tttttccggc
    10261 atcggcttcg caaaataata cccttgcgct ttgtcacatc ccagatcacg aagggcgaca
    10321 gcctcttcct gactttcaac gccttctgct ataatatcca tgttcaggct ctttccaaga
    10381 ccgataatcg ctttgaccag tgctgccgag ccgttatttg ccagcatgtc tttaacaaag
    10441 ctacgatcaa ttttgagagt atcaatcggg aaataatgca gataactcaa tgaggaataa
    10501 cctgttccga agtcatcgat tgaaatgctc attccggcct tttggcacat ggatagggtt
    10561 tctttggcat tatccggttg gcccatcagc agccgctcag tgatttccag atgcacctgc
    10621 gacgcactga cgtcactgag gctgattgtt tcataaacac tgtcaacaaa atcatccgca
    10681 gcaaaatccc taccggagaa gtttacgctc ataaaaagct catggtcata tcccgcgcgg
    10741 ttttcaatgc gtgataaagc catcagcgat tctttcagcg cccaggctga agctttgacg
    10801 atcaggccgc tatcttcaat gatagggata aataccgcag gcgagatgaa gcccttttca
    10861 ggatgttccc aacgcataag cgcttcaaaa cctgcgattt taccgctatc cagcataata
    10921 acaggttggt aatatagtga aatttcatga cgtttaagag cggattcaaa atcgttggtg
    10981 attttaatcg tttcaacagc gctggaacct tccgcataag taagttccaa atcctctgcc
    11041 gcgttgagct tcggcggctc tccagttatt tcagagcgtt gaagcatatc ccggtagcgg
    11101 gcgaggatga cttttgtcat catatgcaag atagggtcgg cattttcaag ccgcaatgcg
    11161 aaatcctctt tggtaatctc aagaagctcg caatcttccg tcgcgcgcac agtggctgtg
    11221 cgcggggcat cgtccaaaat ggccatttcg ccaatcatag cccccggtcc gcgcgaaccc
    11281 acatattgag tatcgccgtt acgggtttct atcaggattt ctacacggcc tgttttaatg
    11341 atataggcgc atttccccgc gtcgccctga cgcataataa cttcgccgga ttgaaaggat
    11401 ttcttatgtt cgtctttatg catgcaatta aggcttaagc tctgattctt gaatacactt
    11461 agtctggtac gcttgccgaa gaaaatcaat gctcaaatcg ccgggcggat gtataaaaga
    11521 agtgcttgac aagcaggggg gcagttcatt attgtccgcc agtcgctgaa cacgaagggc
    11581 cagagccatg aaagtcacaa attcgctgaa aacgcttaag acaagagatc gcaataaccg
    11641 cgtaattcgc cgtcgcgggc gcacatacgt gatcaacaag aaaaacccgc gcatgaaggc
    11701 gcgccagggc tagtcgtcgc tgcgttcttg cagattctaa aacaaatgtt gaaatctttt
    11761 agaatgcacc caatctcgtg gtgcatttgc tttttgtgtc tgatataacc atcgcaatca
    11821 atgtaacact cagaagtaaa gaactgtatg ttttccggtc tctttggatt tatgtccgcc
    11881 gatatggcga tcgatttggg aacagccaat acactggtct atgtgaaaga tcagggtatt
    11941 gtgctcaatg agccctctgt cgtggcc
//
LOCUS       DAOSWR010000059        11804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3436_length_11804_cov_5.057537, whole genome shotgun sequence.
ACCESSION   DAOSWR010000059 DAOSWR010000000
VERSION     DAOSWR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_11804_cov_5.057537"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1474)
                     /locus_tag="PK513_05740"
     CDS             complement(32..1474)
                     /locus_tag="PK513_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOO81983.1"
                     /translation="MSIDSKIDTFVQPFAEHAANFVFYSVPIGSGHEIKLILVWLVVA
                     SLFFTIYLGFVNFRYFKHAIDLLREPANPDDQTTGQITRFQALATSLSGTIGLGNIAG
                     VAVAISVGGPGAALWMVFMGLFGMSTKFAEVVMGVKYRQFLDPNHPDRVSGGPMYYIR
                     EAFREKNMPLLGGVMGSFFAICCCFGVLGAASLFQTNQVFQQALIITGGEGSFLIGKA
                     WMVGLFMVFIVGLVIIGGIRSIAAVTSKIVPFMGGLYLLTGLFVIGTYYAQLPAALVS
                     IVTASFSPEAGLGGFLGALVMGVQRAVFSNEAGLGSAAIAHAAVKTDQPVTQGLVGML
                     GPFIDTIIVCMVTALVIIITGAYEQGNGMEGVELTSRAFGSVLPWFPYVLFVVVFLFA
                     YSTLLSWAYYGVKAFTYLFGNTDLNEMFYKIIFCIFIVIGASAKLDNVILLTDASVFM
                     MAIPNLIGLYLLAPALKKDLNAYTEKLKAT"
     gene            complement(1546..2034)
                     /locus_tag="PK513_05745"
     CDS             complement(1546..2034)
                     /locus_tag="PK513_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81984.1"
                     /translation="MPRLLSFITLIILLIPTFAMAASCYSPVEAEAEQGIRIHSELMV
                     IGLNCQHMGVRKGDNLYLKYRQFTADHAELFAKYEQILLSHFSKSGTKKPEEKLNALR
                     TNFANKISNDAAAMRPDVFCSRYAPRITKASEMKHNDLRKWASTFYESHPVSKPFCAS
                     GG"
     gene            2156..3532
                     /gene="miaB"
                     /locus_tag="PK513_05750"
     CDS             2156..3532
                     /gene="miaB"
                     /locus_tag="PK513_05750"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOO81985.1"
                     /translation="MPNNVQKSKKLFIKTWGCQMNSYDSNRMSDILRPLGYRVVDTPD
                     DADMVILNTCHIREKATDKVFSDLGRLRPLKEKKEADGGKMLMAVAGCVAQAEGDFIL
                     ERAPYVDMVFGPQTYHELPEMVLKANGNERIVNTDFDGDNKFDKLPEEQKNSGVSAFL
                     SIQEGCDKFCTFCVVPYTRGSEYSRSVQEILNEAKRLVDNGALEIIVLGQNVNAFHGT
                     GPDGSVWGLGQLLRAINEIEGIQRIRYTTSHPRDMDDDLIAAHGELDKLMPFLHLPVQ
                     SGSDKILKAMNRKHTRDLYFDVIEKLRKARPDLAFSSDFIVGFPGETEEDFEDTMNLV
                     RRVEFSSCYSFKFSARPGTPAANMQNLVHESIASERLARLQSLLNEQQIAFNRQSVGL
                     TLPILFDRKGKREGQLVGRSPFNQSVFVEGNERLMNSIADVKISEGYENSLTGNVVTG
                     ETESRVAP"
     gene            3601..4578
                     /locus_tag="PK513_05755"
     CDS             3601..4578
                     /locus_tag="PK513_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO81986.1"
                     /translation="MSTEKVSGQTIEFRKNDLLPLLFGAHNAHLHYLEDRLGIDIADR
                     GNRLTLSGDAGAIKTAEHILNRLWNKIEQNHEVGTAEIDAELRFLEDKEKPKTQEQKD
                     KVMSKDDIVIKTKKKTIVARSPNQALYMRSILDHEMVFGLGPAGTGKTYLAVAVGVAM
                     YLEGKVERLIFARPAVEAGERLGFLPGDMKDKVDPYLRPIYDALHDMMPYDFMVKKIE
                     DGDIEIAPLAFMRGRTLSNAFVILDEAQNTTTTQMKMILTRLGESSRMVINGDLSQTD
                     LPVGVKSGLRDASEILDGIEDIQFIHFQKDDVIRHKLVSKIVNAYENKK"
     gene            4588..5061
                     /gene="ybeY"
                     /locus_tag="PK513_05760"
     CDS             4588..5061
                     /gene="ybeY"
                     /locus_tag="PK513_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOO81987.1"
                     /translation="MNQPIIDISIQHSEWTRAIRDIQNIVISVIGLTLEKADVEAQGK
                     ELSLVLADNDFVQALNKEWRGKDKPTNVLSFPQDEPMLLGDIILAYETVRDEAKEQDK
                     RFEDHATHLIVHGLLHLLGHDHIEEADAQIMESLEIEILRTLNIKNPYDHESFVA"
     gene            5085..6059
                     /locus_tag="PK513_05765"
     CDS             5085..6059
                     /locus_tag="PK513_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO81988.1"
                     /translation="MRIDLENLPLTDTAHTSQDETPDDRSERGCSKKHERPGFLPRLK
                     FLITGRKDRGDDDLREAIEEYIVKPQGFNTDSVSTHERVLLSNILKLRDITVVDEMIP
                     RADIVAIDVDIKQDDLLALLADLQFSRLPVYKETLDDVLGTVHVKDIVAGLSKGERIN
                     LKEMITEIPIVSPSMPILDLVLKMRHSRRHMALVVDEYGGIDGLITIGDVIESIIGEI
                     EDEHDTQDDPQMLEALDGSSVVADARVWLEEFERRYGHILDEEEREESDTLGGLVFYL
                     AGRVPARGEVLTHSNGMMFEILDADPRRINRLKITRIPQNPDLAQDGE"
     gene            6075..7631
                     /gene="lnt"
                     /locus_tag="PK513_05770"
     CDS             6075..7631
                     /gene="lnt"
                     /locus_tag="PK513_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOO81989.1"
                     /translation="MSLPATILLLNLFKRPLQSLTLCFCAGALAALSMAPLNGWPVLF
                     ISLPILYGALVFAPTGKRAFGLGWLFGFGYFAFSLSWIGNALLVEGNPYKWAWPLAVS
                     GLPALLAFFPAFAALASQKIFDLRSVSGWLGFVSIYCGFEWLRGHIFTGFPWNLFGYT
                     WADYLPVLQVLWLSDVYALTWLTILWASALGAAVFLPATQRAWPIVIALISFAGCYGY
                     GLAVLPDTLDFNKDVRVRIVQPDIDQAEKWQPDKMAAHFRKHLQLSENTNGYDLPTII
                     VWPETALSYRLLEEPAAMAELKEMLAAHPKNSVLLTGLLRRDLNDDSYGNSLVMVDRS
                     GTVSNTYDKRHLVPFGEYIPFQRWIPLAPIVQFKGFKAGSGAQTFTTPSGHTYSPLIC
                     YEIIFPGGSIAHDFTPDFIVNVTNDAWYGLSAGPYQHLTQALFRAVETGVPVVRAANT
                     GFSVLISPYGHVEAQSNLFETNRIDIQLPSKRLSQKYAITLRTPLTLLFLLFFISLGI
                     YGGQKFSKKG"
     gene            7781..8203
                     /locus_tag="PK513_05775"
     CDS             7781..8203
                     /locus_tag="PK513_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO81990.1"
                     /translation="MHKKTKGTPSEVDVHVGQRLRVRRALLGLSQEKLADALGLTFQQ
                     IQKYERGTNRVSAGRLYALSKILDVPISYFFEQFGKPQDTQALSGMADNTQQAFGGED
                     LMNNKETLDLVRTYYSVQDPKARKDILKFIKSMAEKIS"
     gene            8323..9567
                     /gene="metK"
                     /locus_tag="PK513_05780"
     CDS             8323..9567
                     /gene="metK"
                     /locus_tag="PK513_05780"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOO81991.1"
                     /translation="METDMLENTAPALQEHEHPDSRPELKDYIFTSESVSEGHPDKVC
                     DRISDTVVDLFLTADPYSRVAAETLVTTDTVIIAGETRGPESITKDVIEEAARAAIKD
                     IGYEQEGFHWKTANVQILLHKQSADIAQGVDEGTGVDTNEGAGDQGIMFGYACRETKA
                     LMPAPIHFSHEILRALAEARKGGPLEGKIEPDSKSQITLEYRDDKPVRATSVVVSTQH
                     ADHLSQDEVKELVRPYVEKTLPEGCMPPEEEFYVNPTGRFVIGGPVGDAGLTGRKIIV
                     DTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNVVAAGYADGCTIQLAYAIGVSK
                     PLSVYVNTHGTGSAPEQEIINALIKLVDLSPRGIREHLQLNKPIYARSAAYGHFGRTP
                     TADGGFSWEKTDLVDDLRKILG"
     gene            9560..10258
                     /locus_tag="PK513_05785"
     CDS             9560..10258
                     /locus_tag="PK513_05785"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HOO81992.1"
                     /translation="MDNPSPQKRVFGRRHGRPLNKSRTEVIETLLPKLSVPVEMLKED
                     GTLGPSSLFEAPYKECWFEIGFGNGEHLSALMRRHPEHAYFGAEPFINGMAAFLKDIQ
                     NDLQTRIRVHMDDAMMLAGSLEDECLDGIYILNPDPWHKKRHHKRRIVNRENLKCFHR
                     ILKPGGQLILSTDVPYLAEWMITEVMIHGGFTWLANSKRDWETKLEDWIDTAYQTKGA
                     KGADKMVYLFFSRT"
     gene            10312..11439
                     /locus_tag="PK513_05790"
     CDS             10312..11439
                     /locus_tag="PK513_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81993.1"
                     /translation="MKSFIFLAFILVLGVFAFSASYAEETNSVEQTDLWGDDTGQYGY
                     QDYLKDLKTNPSLELTEEICKNSHGAKNSASGEPTRRAFARKHLERDECYMALMKQEG
                     QTTLLRCLQLSENKQPLCLKNITIQEDNPEICNFIGAENEKTHANCLTYFAGKKSFKE
                     DNTNFKPCRNIQDVENRYSCYRTTLLSRYQSQMDLAAVHVSIPPTQQKYAKEIKDCGQ
                     FESLFNQQQCYHSIPHNFYAEAGYTNCSYLEIHPCKKARDRGFLYLMMAGDKRADKIA
                     QIYGYGVLTAQEHNARDPNYKIFVQDLTQFLDNLTHLPKDLELFCQAAMPIKTYEQWI
                     NVKNFEHVRVRNVFCNRFPYLKTKNWYWEEMDKKLGFSEKQ"
     gene            11533..>11804
                     /locus_tag="PK513_05795"
     CDS             11533..>11804
                     /locus_tag="PK513_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81994.1"
                     /translation="MHVVFKIIKFVLLAFASIVGLFVLAFLIVPHFIRYYDYGINKNK
                     TDLPYPSYFYEDNLQHDFSTVLHTRTGTFNKQYDALIYMQHMDSPV"
BASE COUNT         3184 a         2489 c         3034 g         3097 t
ORIGIN      
        1 aagcggtgat tgtggataac ttattatgtg gttatgtggc ttttaatttc tcggtgtagg
       61 cattgagatc ttttttcaat gcgggagcga gtaaatataa gccaatcagg ttggggatcg
      121 ccatcatgaa gacggatgcg tctgtgagca ggataacgtt atcgagcttg gcggatgcgc
      181 caatgacaat gaaaatgcag aagatgattt tatagaacat ttcgttgagg tcagtgttac
      241 cgaataggta ggtgaaggct tttacgccgt aataagccca tgacagtaaa gttgaatacg
      301 cgaacaggaa gacgacgaca aacagcacgt aagggaacca tggcaatacg ctgccaaagg
      361 cacgggaggt cagttctacc ccttccatgc cattgccctg ttcgtaagca ccagtgatga
      421 tgatgaccaa tgccgtgacc atgcacacga taatggtatc gatgaagggg cctaacatac
      481 cgacaagccc ttgggttaca ggctgatctg ttttgacagc ggcgtgagca atggcagcag
      541 agccgagtcc tgcctcattg gaaaaaactg cgcgctgcac acccatgacg agcgcaccga
      601 gaaaccctcc caaaccggcc tcaggagaaa atgaggctgt tacaatactg accagcgcgg
      661 cggggagttg tgcataatat gtgccgataa caaataatcc ggtcaaaagg taaagcccgc
      721 ccatgaaagg gacgattttg ctggttacgg cggcgatgga acggatgcca ccaatgataa
      781 caagcccgac aataaagacc atgaacagcc caaccatcca ggccttacca atcagaaagc
      841 tgccctcgcc gccggtaatg atcagggctt gctggaacac ctgattggtt tgaaacaggc
      901 tggccgcacc gagtacgcca aagcagcagc aaatggcaaa gaagcttccc atgacgccgc
      961 cgagcagcgg catgtttttt tcgcgaaaag cctcacgaat gtaatacatc ggcccgccag
     1021 atacgcgatc cgggtgattg ggatcgagaa actggcggta tttgaccccc attacgacct
     1081 cggcaaactt agtcgacata ccgaacaagc ccataaagac catccacagc gctgcgccgg
     1141 ggccgccgac cgaaatagcg actgctacgc ctgcgatatt tcccagccca atagtgccgg
     1201 agagcgaagt ggcaagtgcc tgaaatctgg taatctgacc tgtcgtctga tcgtcaggat
     1261 ttgcgggttc gcgtagcaga tcgatcgcgt gtttaaaata acgaaaattg acgaagccga
     1321 gatagatggt aaagaacagc gaggcaacaa cgagccagac caggatcagc tttatttcat
     1381 ggccactgcc gataggtacg gagtaaaata caaagtttgc cgcatgttcg gcaaagggct
     1441 gaacgaatgt gtcaattttt gaatcaatac tcataggttt agcgttgcgt ttacaggcct
     1501 atatttaaag aaaaaagcct tgagcccata aaaatatcca cagatttatc ccccggatgc
     1561 gcagaatggt ttagatacgg gatgcgattc ataaaatgtt gacgcccatt tgcgcaggtc
     1621 gttgtgtttc atttcggatg ctttcgtaat gcgcggggcg tagcgtgagc agaaaacatc
     1681 agggcgcata gcggctgcat cgttagaaat tttgttggcg aaattggtgc gcagcgcgtt
     1741 gagcttttcc tcaggctttt ttgtgcctga ttttgaaaaa tgggacagga gaatttgttc
     1801 atattttgcg aacaattccg catgatcggc ggtaaattgc cgatatttga gatataaatt
     1861 atcacccttg cgcacgccca tatgctggca gttcaggccg ataaccatga gttcgctatg
     1921 gatgcgaata ccttgctcag cttcagcctc aaccggtgaa taacaagagg cggccattgc
     1981 aaaagttgga ataagcaaaa tgatcagcgt gataaaggat aaaagtcgtg gcatgcaatg
     2041 tccttgatga acgggtcaaa ttgaagctct gcttatagtc taatgatgcc atatttgtcc
     2101 agttttctga tgataaatct tgattttcca atgaagtctg tgatttaaag cctgcatgcc
     2161 aaataacgta cagaagagca aaaagctatt tattaagacg tggggctgtc agatgaacag
     2221 ttatgacagc aatcgcatgt ctgatattct gcgaccactg ggctatagag tcgttgatac
     2281 gccagacgat gcggatatgg tgattctcaa tacctgccat attcgggaaa aagccaccga
     2341 taaggtgttt tcagatttgg ggcgcttgcg tcctttgaaa gaaaagaaag aggcggacgg
     2401 tggcaagatg ctgatggcgg ttgcaggttg cgtggcccaa gctgaaggtg atttcatttt
     2461 agaacgcgcg ccctatgtag acatggtgtt tgggccgcag acctatcatg aattgccaga
     2521 gatggtgctc aaagccaacg gcaatgagcg aattgtgaat accgactttg acggagacaa
     2581 caagttcgac aagctgcccg aagagcaaaa aaattccggc gtatcggcgt ttctttctat
     2641 tcaggaagga tgcgataaat tttgcacgtt ttgcgtggtg ccctatacgc gcggcagtga
     2701 atattcacgc agcgttcagg agatattgaa tgaggccaag cggctggtcg ataacggcgc
     2761 actcgaaatt attgtgctgg ggcagaatgt gaatgcgttt cacggcaccg gaccggatgg
     2821 cagtgtttgg gggctgggac agcttttacg cgcgattaac gagattgaag gcatacagcg
     2881 catccgttat accacttcgc acccgcgtga tatggacgat gatctgattg cggcacatgg
     2941 ggaactggac aagctgatgc cgtttttgca tttgccggtg cagtcgggct ctgacaaaat
     3001 tttgaaagcg atgaaccgca agcatacgcg cgatttgtat tttgatgtga ttgaaaagct
     3061 acgcaaggcg cggcctgatc ttgccttttc atcggacttt attgtcggtt ttcccggtga
     3121 aacggaagaa gatttcgaag acacgatgaa tctggtacgc cgggttgagt tttcttcgtg
     3181 ctatagtttt aaattctccg cccgtcccgg tacgccggcg gcgaatatgc aaaatcttgt
     3241 acatgagagc attgcctctg agcgtctggc gcgtttgcag agtttgctca atgaacagca
     3301 gattgccttt aaccgccaga gcgttggcct taccctgccc attctgtttg atcgcaaggg
     3361 caaacgcgag gggcagcttg tggggcgcag cccgtttaat caatcggtgt ttgttgaggg
     3421 caatgaacgt ctgatgaatt caatcgctga tgttaagatc agtgaggggt atgaaaactc
     3481 actgaccggc aacgtggtga cgggggaaac cgagagccgg gttgcaccct aaataaaatg
     3541 gaaatcttaa catttttgtt gtaaaattaa tgtatcagaa actggcaaag gaaaagctgt
     3601 ttgagcactg aaaaagtttc cggtcaaact attgagttca ggaagaacga cctcctcccc
     3661 ctgctgtttg gtgcgcataa tgcgcatctg cattatcttg aagacaggct tggtattgat
     3721 attgccgatc gcggtaaccg gcttacgctt tcgggtgatg ccggggctat aaaaactgcc
     3781 gagcacattc tcaatcgttt atggaataaa attgaacaaa atcatgaggt cggcacagca
     3841 gaaattgatg cagagctgcg ttttttagaa gataaagaaa agccgaaaac acaggaacaa
     3901 aaagacaagg ttatgtccaa agatgacatt gtaattaaaa ccaagaagaa gacgattgta
     3961 gctcgctcgc ctaatcaggc gctctatatg cgctctattc ttgatcatga aatggtgttt
     4021 ggacttggcc ctgcggggac cggaaagaca tatctggctg tagccgtggg tgttgcgatg
     4081 tatcttgaag gcaaggtgga gcgtttaatc tttgctcgcc ctgccgtaga agccggggag
     4141 cggctgggct ttttacctgg cgacatgaag gataaagttg atccatattt gcgaccaatt
     4201 tatgacgcgc tgcacgacat gatgccatat gattttatgg ttaaaaaaat cgaagatggg
     4261 gatattgaaa ttgcaccgct ggcctttatg cgcgggcgca ccttaagcaa tgcatttgtg
     4321 attctggatg aggcacaaaa caccacgaca acgcagatga agatgatttt gacacggctg
     4381 ggggagtcct cacgcatggt gataaatggc gacctttcgc agaccgattt acctgtggga
     4441 gtgaaatcag gcctgcgcga tgcgagcgag attcttgacg gcattgaaga catccagttt
     4501 attcacttcc aaaaagatga tgtcattcgt cataagttgg tgagcaagat tgttaatgct
     4561 tatgagaaca agaaatagcc ttactttatg aaccagccca ttattgatat atcaatccag
     4621 catagtgaat ggacacgcgc gatacgcgat attcaaaata ttgtgatttc cgtgattggt
     4681 ctgacattgg aaaaagccga tgttgaggcg cagggtaaag agctgagcct tgtgctggcg
     4741 gataatgatt ttgtgcaagc gctgaataag gaatggcggg gcaaggataa accgaccaat
     4801 gtgctgtcct tcccgcaaga cgagccgatg ctgctgggtg atattattct ggcttatgaa
     4861 accgtgcgcg atgaagccaa agaacaggat aagaggtttg aagatcatgc gacgcatttg
     4921 attgtgcatg gacttttgca tttgcttggg catgatcata ttgaagaagc agatgcgcag
     4981 atcatggaat cgcttgaaat cgagatatta agaacgttga atataaaaaa cccttatgat
     5041 cacgagagtt tcgtggcata atccttgcca aagacatgtt ggaaatgcgt atagatttgg
     5101 aaaatttacc cctcactgat acagcacaca cgtctcagga tgaaactcct gatgacagga
     5161 gtgagcgtgg ctgtagcaaa aaacacgaac gccccggttt tcttccccgt ttaaaatttt
     5221 taattacagg ccggaaagac agaggtgatg atgatcttcg tgaagccatt gaagaatata
     5281 ttgtcaagcc acagggcttt aacaccgatt ctgtttcgac gcatgaacgc gttttgcttt
     5341 ccaacattct gaagctccgc gacatcactg ttgtagatga aatgatccca cgcgccgata
     5401 ttgtcgcgat tgatgtggat ataaagcaag acgatttgct ggcattgctt gcagatttgc
     5461 aattcagccg tcttcctgtt tacaaggaaa cgctggacga tgttttaggg acagtacatg
     5521 tcaaagatat cgttgcgggt ctgagcaagg gcgaaaggat caatcttaaa gaaatgatta
     5581 ccgagattcc gatcgtttcc ccttcaatgc caatccttga tctggttctg aaaatgcgtc
     5641 attcgcggcg gcatatggcg ctggtggtag atgaatatgg cgggattgac gggcttatca
     5701 cgattggtga tgtgattgag agcatcattg gagagattga agatgagcac gatacgcaag
     5761 atgatccgca aatgctcgaa gcattggatg gttcttctgt ggtggcagat gcccgcgtgt
     5821 ggttagaaga atttgagcga cgctatggac atatactgga tgaagaagaa cgtgaggaaa
     5881 gtgatacgct gggggggctt gtattttatc tggcaggtcg ggtgcctgcg cgcggagaag
     5941 ttcttaccca ttctaacggt atgatgtttg aaattctaga tgctgatccg cgccggatta
     6001 accgtctaaa aatcacacgc ataccgcaga accctgacct tgcgcaggat ggtgaatagc
     6061 tctacaaata ttgaatgagt ttgcctgcca caattcttct gttgaattta tttaagcgcc
     6121 ctttgcaatc attgacgctg tgcttttgcg caggggcttt ggccgctctg tcgatggcac
     6181 cgctcaatgg ctggccggtt ttgtttatca gcctgccgat tttgtacggt gcattggtat
     6241 ttgcgccaac cggaaagcgt gcgtttggtt taggctggct gtttggtttt ggctattttg
     6301 cgttcagcct gtcgtggatc ggcaatgcgc tgctggttga aggtaatcca tataaatggg
     6361 cctggccgct ggctgtgtcg ggactgcctg ccctgcttgc attttttcct gcttttgccg
     6421 cccttgcatc acaaaagatc tttgacctgc gcagcgtaag tggatggctc gggtttgttt
     6481 cgatatattg cgggtttgaa tggctacgcg gtcatatttt cacaggcttt ccgtggaatt
     6541 tatttggtta tacgtgggct gattacctgc cggtgctgca agtgctatgg ctcagcgatg
     6601 tatacgcact tacctggttg acaattttat gggcaagcgc cttgggggcc gctgtttttc
     6661 ttcctgcgac tcagcgcgca tggccgattg ttattgctct gatcagtttt gccgggtgct
     6721 atgggtatgg cttggctgtt ttgcctgata cacttgactt taacaaggat gtacgggttc
     6781 ggattgtgca gcctgatatt gatcaggctg aaaaatggca accggataaa atggcggcgc
     6841 attttcgcaa acatctgcag ctttctgaaa acacgaatgg ttatgacctg ccaacgatta
     6901 tcgtctggcc ggaaacagca cttagttacc gactgcttga ggaaccggcg gcgatggcgg
     6961 aattaaaaga gatgttggcg gcgcacccga aaaattctgt tttactcaca ggtttattgc
     7021 gccgtgacct aaatgacgac agttacggca attctttggt gatggtagat cgaagtggca
     7081 cggtttcaaa tacctatgat aagcgccatt tggtgccgtt tggtgaatac atcccttttc
     7141 agcgttggat tccgctggcg ccgattgtgc aatttaaagg ctttaaagcg ggatcagggg
     7201 cgcagacatt cacgacccct tccgggcata catacagccc gttgatttgc tatgaaatca
     7261 tttttccggg agggagtatc gcgcatgatt tcactccgga ttttatcgtc aatgtcacca
     7321 atgacgcatg gtacgggctt agtgccggac cgtatcaaca tcttacgcaa gctcttttta
     7381 gagcggttga aaccggggtg cccgtcgttc gtgcggccaa tacggggttt tctgttttga
     7441 tttctcccta tggacatgtc gaggctcagt cgaatctttt tgaaacgaat cgaatcgata
     7501 tacaacttcc ttcaaaaaga ctttcacaaa agtatgctat tacattgcgt acaccactaa
     7561 ccctattatt tttattgttt tttatatcgc ttggaatcta tggtgggcaa aaattttcaa
     7621 agaaaggttg atttttttgc acatagacat acctaaggtt agttgcatac actagtaatc
     7681 atggggtatt tctttttaga gagtttcaca gtgcgttttt ttaaagccac gatgatactt
     7741 aaataataaa cgtaaatact gctttaggat agggttttat atgcacaaga aaacaaaagg
     7801 caccccaagc gaggtcgatg ttcatgtcgg gcagcggttg cgtgtgaggc gggctttact
     7861 gggattaagt caggaaaaat tagccgatgc attggggcta acctttcaac agatccaaaa
     7921 atacgagcgt ggcacaaaca gagtcagtgc cggacgtttg tatgccctta gcaaaattct
     7981 tgatgtgcct atcagctatt tctttgagca atttggtaaa cctcaagata cacaggccct
     8041 cagtggcatg gctgacaata cgcagcaagc ttttggtggt gaagatttga tgaataataa
     8101 agagactctt gatcttgtac gcacgtacta ttctgtgcag gaccctaaag cgcgaaaaga
     8161 tattcttaaa ttcattaaat ccatggcaga aaagatatct tagttttttg ttcttcgttt
     8221 tgcatagact ttcagcttgc catggccggt cttggtcact aatgttgggt ttttgaaagg
     8281 tcaaaagcaa acgagcagcc ttatcgatta aatatgtaac gaatggaaac agacatgcta
     8341 gaaaacacag cccctgccct gcaagaacat gaacatccag attcacgtcc tgaactgaag
     8401 gattatattt ttaccagtga atcagtttct gagggccacc cggataaagt ctgtgaccgc
     8461 atttccgata cggttgttga cctattcctc accgcagacc cttattcacg cgtggcggcg
     8521 gaaacgctgg ttacaactga tacggttata attgctggag aaacgcgcgg acctgagagt
     8581 attaccaaag acgtgataga agaagcggcg cgcgcggcca ttaaagatat tggttacgag
     8641 caggagggct ttcattggaa aaccgcaaac gtacaaatcc tgttgcataa acaatctgct
     8701 gatattgctc aaggcgttga tgaaggcaca ggcgttgata ccaatgaagg tgccggcgac
     8761 caggggatta tgtttggtta tgcatgcagg gaaaccaaag ctttaatgcc cgcgccgatt
     8821 catttctcgc atgagatttt gcgggcactg gctgaggcgc gtaagggcgg gccgctggaa
     8881 ggtaagattg aaccggattc aaagtctcaa atcactttgg agtatcgtga cgacaaacct
     8941 gtgcgtgcga cttctgttgt ggtttcgacg cagcatgctg atcatttgtc gcaagacgaa
     9001 gttaaagagc tggtgcgtcc ttatgtagaa aaaacattac ccgaaggatg tatgccgcca
     9061 gaagaggaat tctatgtgaa ccccaccggg cgctttgtga ttggcgggcc ggttggcgat
     9121 gcaggcctga ccggtcgcaa gattatcgtg gatacttatg gcggtgctgc tccgcatggt
     9181 ggtggtgcat tttccggaaa ggatccgacg aaggttgacc gctctgcggc gtacgctgca
     9241 cggtatttgg ccaaaaacgt tgtggcagcg gggtacgctg atggctgtac gattcaactg
     9301 gcttatgcga ttggcgtatc taaaccctta tccgtgtatg taaacacgca tggaacagga
     9361 tctgcgccgg agcaggagat tatcaatgcg ctgatcaagc tggttgatct tagtccgcgc
     9421 ggcattcgtg agcatttaca gcttaacaag ccgatttatg cgcgtagcgc agcttatggc
     9481 cattttggcc ggacgccgac ggccgatggc ggattttcat gggaaaaaac cgacttggtt
     9541 gatgatttgc gcaagattct tggataaccc atcgccacag aaacgagtct ttgggcggcg
     9601 tcacggccgc cctttgaata agagccggac ggaagtgatt gaaacgcttt tgcctaagct
     9661 gtctgtgcct gtggaaatgc tgaaggaaga tggtacgctg gggccctcct ctttgtttga
     9721 agcgccatac aaagaatgct ggtttgagat cggatttggc aatggtgagc atttaagcgc
     9781 gttgatgcgt cggcatcctg agcatgcgta ttttggggcc gagccgttta ttaatggtat
     9841 ggccgctttt ctcaaagata ttcagaacga tctgcagacc cgtatccgcg tacatatgga
     9901 cgatgcgatg atgctggcgg gttcattgga agacgaatgt ctggacggga tatatattct
     9961 caaccccgat ccgtggcata aaaagcgcca tcataagcgc cggattgtga atcgtgaaaa
    10021 tttgaagtgt tttcaccgca ttttaaaacc cggtgggcaa ttgattttaa gcaccgatgt
    10081 gccgtatctg gctgaatgga tgattacgga agtgatgatt catggtggct tcacgtggct
    10141 ggcaaacagt aaacgtgact gggaaactaa gcttgaggac tggatcgata cggcctatca
    10201 gaccaaaggg gccaaaggcg cggacaagat ggtgtatttg tttttttcac ggacataaat
    10261 ttgcctcaaa atccaacccg tgagacaatg aactttgagg ggacggcgca catgaaatct
    10321 tttatttttt tagcatttat cctggttttg ggcgtttttg cattttctgc ttcctatgca
    10381 gaagagacga attctgtgga acaaacggat ctatggggag atgacactgg gcaatacgga
    10441 tatcaggact atctgaaaga tcttaaaaca aacccttctt tggaacttac cgaagagatt
    10501 tgtaaaaaca gccatggagc gaaaaactcg gcatccgggg aacctacccg aagggctttt
    10561 gctcgcaaac atctcgaacg cgatgagtgc tatatggcgc tcatgaagca ggaaggacaa
    10621 acgacccttt tgcggtgtct gcaattatcg gaaaacaaac aaccgctttg tcttaaaaac
    10681 attacaattc aggaggacaa tcctgaaatt tgcaatttta taggtgcgga aaatgagaaa
    10741 acccatgcga attgcctaac ttattttgca gggaagaaaa gctttaaaga agacaataca
    10801 aactttaagc cctgccgcaa tatacaggac gttgaaaacc gttactcatg ttatcgaact
    10861 acccttttat cccgctacca aagccagatg gatttagcag ctgtacacgt gagcattccc
    10921 ccgacgcaac aaaaatatgc caaagaaatt aaagattgcg ggcaatttga gagcctgttt
    10981 aatcagcaac aatgctatca ttctattcct cataattttt atgccgaagc gggctatacc
    11041 aattgcagct atttggaaat acatccttgc aaaaaagcaa gagacagagg tttcctctat
    11101 ctcatgatgg cgggagacaa gcgcgccgac aaaattgcgc aaatatacgg ctatggcgtt
    11161 cttacagccc aagaacacaa tgcaagagac ccaaactata aaatttttgt ccaggacctt
    11221 acacaatttc ttgataacct tacccatttg ccaaaagacc ttgagctttt ttgccaggcc
    11281 gccatgccga taaaaaccta tgagcaatgg atcaatgtta aaaactttga gcatgttcgc
    11341 gtgagaaatg tcttttgcaa ccgctttcct tacctgaaaa ccaagaactg gtattgggag
    11401 gaaatggaca agaaactcgg tttttctgaa aaacagtaga tttttgttct taaaaggagt
    11461 gcgcgaaagg tgcggataag atggtatatt tgctgtttga taaaaaacaa actctagacg
    11521 attgttaatc tcatgcatgt tgtttttaag attataaaat ttgtacttct ggcattcgcc
    11581 tcgattgtgg gactttttgt gctggcattt cttattgtac cccactttat ccgttattat
    11641 gactatggca ttaacaaaaa caaaactgat cttccatacc cgtcttattt ttacgaagat
    11701 aatttacaac acgattttag caccgtactg cacacacgca ccggcacatt taacaagcaa
    11761 tatgatgcgc ttatttatat gcaacatatg gatagcccgg tcag
//
LOCUS       DAOSWR010000060        11687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3478_length_11687_cov_4.405863, whole genome shotgun sequence.
ACCESSION   DAOSWR010000060 DAOSWR010000000
VERSION     DAOSWR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_11687_cov_4.405863"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /gene="lptD"
                     /locus_tag="PK513_05800"
     CDS             complement(<1..1469)
                     /gene="lptD"
                     /locus_tag="PK513_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOO81995.1"
                     /translation="MRKILIVSVFLICALANGPARAQLLTGYQPVASEVEVSGVPEAE
                     AAVPVEVYVPTPRVVPKSSAPLRFQIASASGEGFSSQYSPLLTQSYDLERGDSAQRDG
                     EAPVDIQADQMLRDELSNTVSASGDVMIVQAGRILRADDVLYDLGIDRVQADGNVVLN
                     EENGDIHLTDYAAYSNALRDGDVERLRSVLNDGSRFTAERGRLEGGTKITMEGASYTP
                     CEPCEEKPDRPPVWRIVASEVEHDQEVHRVSYRHARFEALGVPIAYTPYFSHPDGTIK
                     QKSGFLSPSFGYKSELGAFVENSYYWAIAPDHDATFGLMAMTKQAPLGLAEYRKRLKD
                     ASLELSGGVTSSDRTDRTAGQDVAEEDEMRGHVFAEGRWDIDEKWRAGMNVNWASDDQ
                     YMRQYDFTDEDVLKNELYAERFSGRDYAAVRLLSFQDIRIRETPLDQPGILPEMLASF
                     QGEPGAVPWVKGQWYADASALGLRRERGEQDVQRLSVGSG"
     gene            complement(1564..2922)
                     /locus_tag="PK513_05805"
     CDS             complement(1564..2922)
                     /locus_tag="PK513_05805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO81996.1"
                     /translation="MSFEELGLSENILKAIADCGYEKPTPIQEKAIPNILMMRDVVGL
                     AQTGTGKTASFTLPMIEILHGGRAKARMPRSLILTPTRELAAQVAENFDTYGKYTKLS
                     KALLTGGTSMGDQIKLLERGVDVLIATPGRLLDLFERGQILLTDIKVLVIDEADRMLD
                     MGFIPDIEKIVSLVPFTRQTLLFSATMPPEIRKLTEKFLSSPKEVSVARAATTAKNVE
                     QFVVHTDPRRKREVLGKIIEQENVQTAFIFCNRKRDIDGLVQWLKGKHYNVRGLHGDM
                     PQKIRTETLEAFKNNEITLLVCSDVAARGLDVDDLSHVFNFDVPMNADDYVHRIGRTG
                     RAGKDGRAWMLATRAEQKYLDAIEKLIKEIIPEGVVGGSGPAKKDVSNADGAERDGPK
                     DKPQKSRAQFESNVARKSEEKRDKRLGKPVSKGCKPRRDDDQGDVKGFGDEMPSFFGK
                     AE"
     gene            2999..3964
                     /gene="gshB"
                     /locus_tag="PK513_05810"
     CDS             2999..3964
                     /gene="gshB"
                     /locus_tag="PK513_05810"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOO81997.1"
                     /translation="MSLKVALQMDHPASLNYKKDSSFLIGLEAQKRGHKLFYYSPLEL
                     SLKNGKLSAPCAPLQFRRKKDAYFTLGAFEETDLTTFDIILMRQDFNNPSLYNTITHI
                     LDHISDQTLILNDPTGVRESPEKLLITHFPELAPPTLITRNLDKIREFQKKHPNLILK
                     PLNGFGGMDVYHIKPNDPNLQAVFEMFCRLHPEPFVVQKYLPEIRKGDKRLIVVEGEP
                     IAALLRVPQENNARANLAAGGSAAIGKITPRDREICNMLKPELVRRGLVFVGLDVIGN
                     YVTEINPKSPTGLQQIYKLQGIKCEEAIWNAYEARYIQRQKTSRT"
     gene            3961..4410
                     /locus_tag="PK513_05815"
     CDS             3961..4410
                     /locus_tag="PK513_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO81998.1"
                     /translation="MNEKSKNVKKWLKNQSKSRLIPAGLIVFALLYITLGPRFGREYQ
                     IQIYNELQLAAAICKNSRKWALTNPGRLKLGQGDLRHIPVNGRHIELKYVDYPKVMFL
                     DSGIKKTTGRRTAQDIYCVYADPRVAGDRKYYKYDEQIWVDKVRFRR"
     gene            4422..4847
                     /gene="hisI"
                     /locus_tag="PK513_05820"
     CDS             4422..4847
                     /gene="hisI"
                     /locus_tag="PK513_05820"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOO81999.1"
                     /translation="MNNKKQDEETLTFLTRFDANGLIPCITTSAKTGKVLMLAWMNEQ
                     AIRKSIETGQAHYWSRSRQKLWHKGESSGNTQKIIHMRTDCDQDCLWISVEMNPDASC
                     HTGRESCFYREIDLQADANSGQMKFIDVNRIFNPEDVYS"
     gene            complement(4838..5266)
                     /locus_tag="PK513_05825"
     CDS             complement(4838..5266)
                     /locus_tag="PK513_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82000.1"
                     /translation="MQEKHQKRLYLAILLSESSHIFCCVLPTLFSLVSVLTGLGLMVA
                     VPGWLVSFHDVMHEWEVPMIIASATVVALGWALYFYSKHIDCHDTGCCHEPCGPRKRT
                     ANKILVVASLLLVFNVSIYFGVHRGVDSQDLASVHEHSHE"
     gene            complement(5295..7094)
                     /gene="recJ"
                     /locus_tag="PK513_05830"
     CDS             complement(5295..7094)
                     /gene="recJ"
                     /locus_tag="PK513_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOO82001.1"
                     /translation="MESLSLNKAHWVRPDVDLGAVERVARAYDLPEVVARMLLQRGVE
                     PDDIPGFLNPTLREHFPDPFAMADMGGLAEFVAEAIQDGRKIAIFGDFDVDGATSSAL
                     LYRFLKHCGVEAPIYIPDRLTEGYGPNVEALRSLKDAGAEIVFLLDCGTTAFDVVQAG
                     AEMGLEILILDHHEAETDLPQARYVINPKRKDDAAGLAVLAAVGVTFMACVAINKVLR
                     EAGFFAGDEAPLKSWLDIVALGTVCDMVPLTGVNRLLVRQGFAQMQASENVGLNALIE
                     VSGIKGGIEPYHAGFVLGPRINAGSRVHKSDTGAKLLSCDDPGDASDMAWLLNDCNDK
                     RKALQVEMEREAIAQVEARGLDQNPVIFVSGEGWHTGLSGLVAGRLKEKYGKPACVVA
                     FVKNERGEIEGRGSGRSVSGVHLARAFIDARNEGIIEKGGGHAMAGGFTIGRDRIEDF
                     EVFLDKHIAGQVESGQSAVETVVDGVLTVRGAKTDLVRLVHDFVGPFGQEAPEPVFLF
                     QNVRVHSADVVGETHIRVMISDWEGGSRMKAMAFRSVGTPLGEALLKQGKAAFDILGM
                     LKIDTWGGQERVEMHIKDAALALEMLAEKFVML"
     gene            7209..7505
                     /locus_tag="PK513_05835"
     CDS             7209..7505
                     /locus_tag="PK513_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82002.1"
                     /translation="MTTTPRALTIFFSTQTSSDEIAKVCTALTSRNYENGVRLSTPMQ
                     NSKRPKQNQATAITFKIEAKKHWLSLAEEKDISNNIPKVAIAHQFHSKPECDAA"
     gene            complement(7502..8518)
                     /gene="glpX"
                     /locus_tag="PK513_05840"
     CDS             complement(7502..8518)
                     /gene="glpX"
                     /locus_tag="PK513_05840"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOO82003.1"
                     /translation="MTQVRKAENMAELDNLMGRNLALEVVRVTEAAALAASKLVGRGD
                     KVMADQVAVDAMRDAFNGLHIRGTIVIGEGERDEAPMLYIGEEVGDGHGPEIDIALDP
                     LEGTDITAAGGPNALAVVAMTDKGGFLNAPDTYMDKIAVGPGIDPNILDLDAPIGDVL
                     VRLAKEKDGEVNDLNVCILDRPRHVDLIRDVRTAGARITLIGDGDVSAVIATTDPDTG
                     IDMYVGNGGAPEGVLAAAALQCIGGSMLGRLVFHTDVERERAAKWEITDLDKIYTTDD
                     LAKGDNVMFAATGVTDGTMLRGVRRYPGGAKTSSIIMRSKSGTVRRVEATHNLRRKTW
                     LKSI"
     gene            complement(8544..9854)
                     /locus_tag="PK513_05845"
     CDS             complement(8544..9854)
                     /locus_tag="PK513_05845"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOO82004.1"
                     /translation="MMAGAEQAPLRLGIAGLGTVGVGVVKIIQVHGDLLARRAGRPVK
                     IMAVSARDANKDRGVDLSGYAWVDNALALAGRADIDVIVELIGGSDGTAKELVERSLA
                     TGKHVVTANKALLAHHGAALAQLAEENNVALTYEAAVAGGVPIIKAMREGFAGNAIHA
                     VYGILNGTCNYMLTAMRETGRDFDVILKEAQEAGYAEADPSFDIDGIDAGHKLALLSA
                     IAFGVRLDFENLKIEGIRKITADDIAAADELGYRIKLLGIAKRLNGQVMQVLEPCLVP
                     RSNPLGMVEDVYNAVYVDCDAAQTSLLTGKGAGEGPTASAVMADIVDLARGHVIPAFG
                     VPAQALEVANWMPIDDIRGMYYLRLQVLDKPGVLADVSAILRDYGVSVEAIIQRCRDP
                     HQPVPLVLTTHEVRHGDMQAACTAFGKLETVVDEPCLMRIETLS"
     gene            complement(9897..10604)
                     /locus_tag="PK513_05850"
     CDS             complement(9897..10604)
                     /locus_tag="PK513_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOO82005.1"
                     /translation="MPQSEEVVEYSDRFVDLSSVVYENTGGRVQLFDLDEHVPAPAYQ
                     PVASVEMDAMEIAPATVMPVGQVYATMPFGSIVSADPSVEIFPFEDMTPVPVSAQMAR
                     PYASSLRGEGYVAVGASDRDTVVVYFGHDSATLNQKGLAKVSRVAEAFNAASGIGLTV
                     EGHASVMANYNSAAQRKVVNLKISMDRAFAVARALIDKGVPADFIRVMAWGDAKPAQN
                     LDGKTPEEAARRVEISR"
     gene            10961..>11687
                     /locus_tag="PK513_05855"
     CDS             10961..>11687
                     /locus_tag="PK513_05855"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOO82006.1"
                     /translation="MSKNKNAQKAPQNDNNAAARKALQRQIETLPDPVALSQALMNAY
                     QQSQPLFMDMLQKMGQTPPSAELPSFDPDPMNVGSAGAHLINAMATDPLKFWDLQITY
                     AQKQAQLWQSSMNKMWGQESKPVIKPQKSDRRFRDDSWNDSVFFDFIKQYYLLACNFV
                     EQNIENAEGLSENEKRKLTFTSKLMMDALSPSNFAMTNPEVLKETLQTGGMNLVHGLQ
                     NMQEDFARGNGQLKISTTDYDAFK"
BASE COUNT         3306 a         3698 c         2169 g         2514 t
ORIGIN      
        1 ccactaccaa cgctcaaacg ctgcacatcc tgctcgcctc tttcacgacg caatcccaaa
       61 gcggaagcgt ccgcatacca ctgtcccttg acccaaggca cagcccccgg ctccccctga
      121 aagctcgcca gcatttctgg taaaatacct ggctgatcca gcggcgtttc acgaatgcga
      181 atatcctgaa aagacagcag ccgcaccgcc gcataatcgc ggccagagaa acgctctgca
      241 tataattcat tctttaacac atcttcatcg gtaaagtcat attgacgcat atactgatca
      301 tcagaggccc aattaacatt catccccgca cgccatttct catcaatgtc ccatcgccct
      361 tcagcaaaaa catgtccgcg catttcatcc tcctctgcca catcctgacc cgccgtccgg
      421 tccgtccgat ctgaagaggt cactccgccg ctaagctcca gcgatgcatc cttcaaccgc
      481 ttgcgatact ctgccagccc caaaggcgct tgcttcgtca tcgccatcaa gccaaaagtc
      541 gcatcatgat caggcgcaat cgcccaataa taactgtttt caacaaaagc cccgagttca
      601 cttttgtatc caaaagacgg agacaaaaac ccgctctttt gcttaatcgt accatccgga
      661 tgtgaaaaat acggtgtata ggcaattggt acgcccagcg cctcaaagcg tgcatgacgg
      721 taagaaacgc gatgaacctc ctgatcatgc tccacttccg aagccacaat gcgccaaaca
      781 ggtggacgat caggcttttc ttcacaaggc tcacatggcg tataacttgc gccttccatc
      841 gtgatttttg taccgccctc caaacgccca cgctccgctg taaaacggga accatcattc
      901 aacacgctgc gcaaacgctc cacatcgcca tcgcgcaagg cgttgctata agccgcataa
      961 tccgtcaaat gaatatcgcc attttcttca ttcagcacga catttccatc agcctgtacc
     1021 cgatcaatac caagatcgta aagcacgtca tcagcacgca aaatccgtcc agcctgcaca
     1081 atcatcacat ctcccgacgc actcaccgta ttgctcagct catcacgcaa catttggtcc
     1141 gcctgaatat ccaccggcgc ctcaccgtct ctctgagcgg aatcacctct ctcaagatcg
     1201 taactttgtg ttaaaagcgg actatattgc gaagaaaaac cctctccaga cgcagaagca
     1261 atctggaacc tcaatggtgc tgaggatttt ggaacaacgc gtggcgtcgg cacataaacc
     1321 tcaacaggca cagcagcctc agcttcaggc acgcctgaaa cctcaacctc actcgccacc
     1381 ggctgatatc cggtcagaag ttgggcccgc gccggaccat tcgccagcgc acatatcaaa
     1441 aaaacagaaa caatgagaat tttacgcata tatttgactt aaaggtctat gaaaaaggcc
     1501 tgtaacccta ttcttcttcg ctccctaagt gtgcccgtgc ccacaggctc aggtcaagac
     1561 gtatcactcc gccttaccaa aaaaagacgg catttcatca ccaaacccct taacatcacc
     1621 ctgatcatcg tcacggcgtg gcttacaccc tttagaaaca ggttttccaa ggcgtttatc
     1681 ccttttttcc tcagacttac gcgccacatt agactcgaac tgagcacgag acttctgcgg
     1741 cttgtcttta ggtccatcgc gttcagcccc gtcggcattc gaaacatcct tctttgcagg
     1801 cccagagcca cccacaacgc cttcaggaat gatctcttta atcagctttt caatcgcatc
     1861 gagatatttc tgctcagccc gtgttgctag catccatgcc cgcccatctt tacccgcgcg
     1921 ccccgtacgc ccaatccggt gcacataatc atccgcgttc atcggcacgt caaaattaaa
     1981 tacatgcgac aaatcatcca catcgagtcc gcgcgctgcg acatcgctac aaaccagaag
     2041 cgtaatctcg ttattcttaa acgcttccaa cgtctcagtg cgtatcttct gcggcatatc
     2101 gccatgaagc ccgcgcacat tataatgttt acccttcaac cactgcacca gcccgtcaat
     2161 atcacgcttg cgattacaga aaataaacgc cgtttgcaca ttttcctgct caataatttt
     2221 tcccaggacc tctctcttac gccgcggatc agtatgtacg acaaactgct caacattttt
     2281 tgccgttgtc gccgcgcgtg ccaccgaaac ctctttcgga ctgctaagaa atttctctgt
     2341 caatttacgg atttccggcg gcattgtcgc agagaacagc aacgtctgac gcgtaaacgg
     2401 caccagactt acaattttct caatatcggg aataaagccc atatcaagca tccggtcggc
     2461 ttcatcaatc accaaaacct tgatgtcagt cagcaaaatc tgaccgcgtt caaacagatc
     2521 cagcaaccgc cccggtgtcg cgatcagtac atcaacgccc cgctccagca gttttatctg
     2581 atcgcccatt gatgtcccac ctgttagtaa ggccttgcta agcttggtat acttcccgta
     2641 cgtatcaaaa ttttctgcca cctgcgccgc cagttcccgc gtcggcgtca ggatcagcga
     2701 acgcggcatc cgtgccttcg cccgtccgcc atgcaggatt tcaatcatcg gcagcgtaaa
     2761 actcgccgtc ttgcccgtac ctgtctgcgc taaaccaacc acatcgcgca tcatcaggat
     2821 attcggaata gccttctcct gaataggagt cggcttttca tagccacaat cggcaatcgc
     2881 ctttaagata ttttcgctta agccaagctc ttcaaatgac atatattttt tgcttttttg
     2941 ttatgaatac cctagctatt atactttacg cagtaaatca agcgaaagag ccattccgat
     3001 gtcattaaaa gtcgccctgc aaatggatca tcccgcaagc ctgaactata agaaagactc
     3061 aagctttctc atagggcttg aagcacaaaa gcgcggccac aaactctttt actactcccc
     3121 acttgaactt agccttaaaa atggcaagct cagtgcaccc tgtgcaccgc tgcaatttcg
     3181 acgcaaaaaa gatgcgtatt tcactcttgg cgcctttgaa gaaacagatt taacaacgtt
     3241 cgatatcata ttgatgcggc aggattttaa taacccatct ctttacaaca caatcacaca
     3301 catccttgac catatttcag atcaaacatt gatcctgaat gacccgacag gcgtacggga
     3361 atctccggag aagctgttaa tcacgcattt tcctgaacta gctccaccaa cactgataac
     3421 tcgcaatctg gataaaattc gtgaatttca aaaaaaacat cccaatctca tcctcaaacc
     3481 gctcaatggc tttggcggta tggatgttta ccacatcaaa cccaacgatc ccaacctgca
     3541 ggctgtattt gagatgttct gccgcctcca ccccgaaccc ttcgtcgtac agaaatatct
     3601 gcctgaaatc cgcaaaggtg ataaacgcct cattgttgta gaaggtgagc ccatcgccgc
     3661 cctcctccgt gttcctcaag aaaacaatgc ccgcgccaac ctcgcagccg gcggaagcgc
     3721 tgccatcgga aaaatcacgc cccgcgaccg cgaaatctgc aacatgctaa aacccgaact
     3781 cgtccgccgc ggcctcgtct ttgtaggcct cgacgtcatt ggtaattacg taaccgagat
     3841 caaccccaaa tcaccaacag gccttcaaca aatctacaag ctccagggaa tcaaatgcga
     3901 agaagccatc tggaatgctt acgaagcccg ctacattcaa agacaaaaaa catctcgaac
     3961 atgaatgaga aaagcaaaaa cgttaaaaaa tggctcaaaa accagtcaaa atcccgcctc
     4021 atcccggccg gattaattgt cttcgctctg ctttacatca ccctgggacc acgtttcggc
     4081 agagaatacc agattcagat ctataacgaa ctccaactcg ccgccgccat ctgtaaaaac
     4141 tcccgcaagt gggctcttac taaccctggg cgcctcaaac tggggcaagg cgatcttcgt
     4201 catatccccg taaacggcag gcatattgaa ctcaaatacg ttgattaccc aaaagtaatg
     4261 tttcttgatt cgggaatcaa aaaaactacc ggacgacgta cagcacaaga tatttattgt
     4321 gtctatgctg acccgcgcgt agccggagat cgcaaatatt acaaatatga tgaacaaatc
     4381 tgggtcgata aggtccgttt tcgacgatag gaattttaaa aatgaacaac aaaaaacaag
     4441 acgaagaaac cctcaccttt ctcacgcgct tcgatgccaa tggcctgatc ccttgtatca
     4501 ccaccagcgc caaaaccggc aaagtcctga tgctcgcctg gatgaatgaa caggccatcc
     4561 gcaaatctat cgaaacgggg caggcccatt actggtcccg cagccgccaa aagctctggc
     4621 acaaaggcga aagcagcggc aacacacaaa aaattatcca tatgcgaaca gattgcgatc
     4681 aggactgcct gtggatcagt gttgaaatga accccgatgc cagctgccac actggccgcg
     4741 aaagctgttt ttatcgtgaa atcgacctgc aggcagatgc aaattcaggt caaatgaaat
     4801 tcattgacgt aaatcgcatt tttaaccccg aagacgttta ttcgtgagaa tgctcatgca
     4861 cagaagccaa atcttgagaa tccacaccac gatgaacccc gaaataaata ctgacattaa
     4921 aaaccagtag caagcttgcc acgaccagaa ttttattcgc cgttctcttc cgcggcccgc
     4981 acggttcatg acaacaccca gtatcatgac aatcaatgtg tttgctgtaa aaataaagcg
     5041 cccagcccaa agccaccaca gttgcagagg caataatcat cggcacttcc cattcatgca
     5101 tgacatcatg aaaagaaacc agccaccccg gcaccgctac catcaacccc agccccgtca
     5161 gaacactgac cagactgaac aatgttggca acacacagca aaaaatatga ctactctccg
     5221 agagtaaaat ggccaaataa agacgttttt gatgtttttc ctgcatatat gcacctctaa
     5281 aagagacgcg cagactatag cataacaaat ttttccgcaa gcatttcaag cgccaaagcc
     5341 gcatctttga tatgcatctc aaccctctcc tgcccgcccc aagtatcaat tttcagcata
     5401 ccaagaatat caaacgccgc ttttccctgc ttgagcaaag cttcaccaag cggcgtgcca
     5461 acgctccgaa acgccatggc tttcatacgg ctaccgcctt cccagtccga aatcatgacc
     5521 cgaatatgcg tctcaccaac cacatccgca ctatgcactc gtacattctg aaacagaaaa
     5581 acaggttccg gcgcctcctg tccaaatggt ccaacaaaat catgcaccaa ccgcaccaga
     5641 tcggttttcg ccccacgcac cgtaagcaca ccgtcaacga ccgtttcaac cgcgctctgc
     5701 ccgctttcaa cctgcccggc aatatgcttg tctaaaaaca cctcaaaatc ttcgatccgg
     5761 tctctaccaa tcgtaaaacc gccggccatc gcatgcccac cgcccttttc aatgatcccc
     5821 tcatttcgcg catcaataaa ggcacgggcc aaatgcacac cggaaacaga acgcccggaa
     5881 ccgcgtcctt caatctcacc gcgttcattt tttacaaatg ccaccacaca agcaggcttg
     5941 ccgtattttt ccttcaaacg cccagccacc agaccgctaa gccctgtatg ccagccttcc
     6001 cctgaaacaa aaattaccgg attttgatca agcccccgcg cttcaacttg cgcaatcgcc
     6061 tcacgctcca tctccacctg caacgctttg cgcttgtcat tgcaatcatt aagcaaccac
     6121 gccatatcgc tggcatcacc cggatcatca caagacaaca acttcgcgcc cgtatccgat
     6181 ttatgcactc tgctgcccgc attaatccgc ggccccagca caaatccggc atgatacggc
     6241 tcaatgccac ccttaatgcc cgaaacctca atcaacgcat tcaaacccac attctcactg
     6301 gcctgcatct gcgcgaaccc ctgacgcacc aacaaccggt tcaccccggt caacggcacc
     6361 atgtcacaca ccgtgcccaa cgccacaata tccagccatg acttcaatgg cgcttcatca
     6421 cctgcaaaaa aaccagcctc acgcaaaacc ttgttgatcg ccacgcaggc cataaatgtc
     6481 acgccgaccg ccgccaacac cgccagcccc gctgcatcgt ctttgcgctt cggattgatc
     6541 acataacgcg cctgcggcaa gtccgtttcc gcctcatgat gatccaaaat cagaatctcc
     6601 agccccatct cggcccctgc ctgtaccaca tcaaacgccg tcgttccaca gtccagcaag
     6661 aacacaatct ccgcgcctgc gtcttttagt gaccgtaacg cctcgacatt cggcccataa
     6721 ccttcggtca gccggtcggg tatataaatc ggtgcctcca ccccgcaatg tttaaggaaa
     6781 cgataaagca gcgccgaaga cgtcgcccca tccacgtcaa aatccccaaa aatcgcaatc
     6841 ttgcggccat cttgaatagc ctcggcaaca aattccgcta gcccacccat atccgccatc
     6901 gcaaacggat caggaaaatg ctcccgcaaa gtgggattca aaaaaccggg aatatcatca
     6961 ggctccacac cgcgctgcaa aagcatccgc gcaaccacct ccggcaaatc atacgcccgc
     7021 gcaacgcgct ccacagcgcc caaatcaaca tccggccgca cccaatgcgc tttattcaaa
     7081 gacaaagatt ccataaaaaa gatttaacca cgaataaaca tggatggaca taaattaaag
     7141 gcaaaaaaat attgattttt catataaata tagctataaa acccaaaaat cattacggaa
     7201 acaatattat gaccacgaca ccccgcgccc tgactatctt tttctccaca caaacatcgt
     7261 ctgatgaaat cgctaaagta tgtacagctc taaccagcag aaactacgaa aacggtgtac
     7321 gactttctac accaatgcag aactcaaagc gccctaaaca aaaccaagcc acagctataa
     7381 cctttaaaat agaagcaaaa aaacactggc taagtcttgc agaagaaaaa gacatatcca
     7441 acaacattcc caaagtggct attgcccacc agtttcattc aaaaccggaa tgtgatgccg
     7501 cttaaataga ttttagccac gtcttacggc gtaaattatg cgtcgcctca acccggcgca
     7561 ctgtccccga cttcgaacgc ataatgatcg agcttgtctt cgcgccgcca ggatagcgcc
     7621 gcacgccgcg cagcatagtt ccgtccgtca cgcctgtggc cgcaaacatc acattatcgc
     7681 ccttggccag atcatcagtt gtgtatatct tatccagatc ggtaatctcc catttagccg
     7741 cccgctcacg ctcaacatcc gtatggaaga ccaaacgccc cagcattgaa ccgccaatgc
     7801 actgcaacgc cgccgccgcc aaaacgccct caggcgcacc gccattgcca acatacatat
     7861 caataccggt atccggatca gtcgtcgcaa tcaccgcgct cacgtcacca tcaccaatca
     7921 acgtaatccg cgccccggca gtgcgcacat ccctgatcaa atccacatga cgcggacgat
     7981 caagaataca cacattcaaa tcattcactt cgccatcttt ttccttggcc agcctgacca
     8041 acacatcacc aatcggcgcg tccagatcca aaatattcgg atcaatcccc ggcccgaccg
     8101 caatcttgtc catatacgta tcgggcgcat tcaaaaaacc acccttatcc gtcatcgcca
     8161 caacagccag tgcattcggc ccgccagccg cagtaatatc tgtgccttcc agaggatcaa
     8221 gcgcaatatc aatctcagga ccatgcccat caccaacctc ttcaccgata taaagcatcg
     8281 gcgcttcatc gcgctcaccc tcgccgataa cgatcgttcc acgaatatgc aagccattga
     8341 acgcatcgcg catcgcatcc accgccacct gatcagccat aaccttatcg ccgcgcccca
     8401 cgagcttcga agccgccagc gccgccgctt cagtcacccg caccacctca agcgcaagat
     8461 ttctacccat aagattatcg agttcggcca tgttttctgc tttccgtact tgcgtcataa
     8521 ttataccctt ctattagcgc gccctaactt aacgtctcaa tccgcatcaa gcaaggctca
     8581 tcaacaaccg tctccaattt cccaaacgcc gtacatgccg cctgcatatc gccatgacgc
     8641 acttcatgcg tggtcagaac cagcggcacc ggctgatgcg ggtcgcggca acgctgaata
     8701 atcgcctcaa ccgaaacgcc ataatcacgc aaaatcgccg aaacatccgc cagaacacca
     8761 ggcttatcaa gcacctgcaa ccgcagataa tacatgccgc gtatgtcgtc aatcggcatc
     8821 cagtttgcca cctcaagagc ctgagcaggc acaccaaaag cgggaataac gtgcccgcgc
     8881 gccagatcaa caatatcagc cattaccgcc gaagccgtcg gcccttcccc cgcaccctta
     8941 ccggtcagca acgatgtctg ggccgcatca caatcgacat agaccgcatt atacacatct
     9001 tcaaccatgc caagagggtt agagcgcggt acaaggcaag gctccaaaac ctgcatcacc
     9061 tgcccattta aacgctttgc aatccccaga agcttgattc ggtagccaag ctcgtcagcc
     9121 gccgcaatat catccgccgt aatcttgcga ataccctcaa tcttcaaatt ctcaaaatca
     9181 agccgcacac cgaaggcaat cgccgaaagc agcgccaact tatgccccgc atcaatcccg
     9241 tcaatatcaa agctcggatc ggcttccgca tacccggcct cctgcgcctc tttcaagatc
     9301 acatcaaaat cgcgccccgt ctcacgcatc gccgtaagca tataattgca tgttccgttc
     9361 aaaatgccgt aaaccgcatg aatagcattt cccgcaaacc cttcacgcat ggccttgata
     9421 attggaacgc cccccgccac ggccgcttca tatgtaaggg caacgttatt ttcttccgcc
     9481 aactgtgcca aagcagcacc atgatgcgcg agcaatgcct tattggctgt caccacatgc
     9541 ttccctgtgg ccaaagagcg ctctacaagc tctttcgccg tgccgtcgct accgccgatc
     9601 agttcaacga tcacatcgat atctgcgcgc ccagccaacg ccaatgcatt atcgacccac
     9661 gcatatcctg acaaatcaac accacgatct ttattcgcgt cgcgcgcgct cacagccata
     9721 atcttcacag gccgcccggc ccggcgcgca agcaaatcac cgtgcacttg aataatctta
     9781 accacaccga ccccgactgt ccccagcccg gctattccca aacgcaacgg cgcttgctct
     9841 gctccagcca tcatctcaac ctttcaaatt gtttattaaa tctatacgtg aagcaattaa
     9901 cgagaaattt caacccgccg cgctgcttct tctggtgttt ttccatccag attttgagca
     9961 ggctttgcat caccccacgc catcacccgg ataaagtccg caggaacacc tttatcaatc
    10021 aatgcgcgtg ccacagcaaa cgcccgatcc atagaaattt taagattaac aaccttacgc
    10081 tgcgctgccg aattataatt tgccatcacg ctcgcatgcc cctcaaccgt aagcccgatc
    10141 cccgacgccg cattaaacgc ttcagccact ctggaaactt tcgccaaacc cttttggttc
    10201 agcgttgcag aatcatgacc gaaatacaca accaccgtgt cacgatcaga cgcaccaacc
    10261 gcaacataac cttcgccgcg taaagacgaa gcgtaaggcc gcgccatttg cgcacttaca
    10321 ggcacaggtg tcatgtcttc aaacgggaaa atctctacac tcggatcagc ggaaacaata
    10381 gagccaaaag gcattgtggc ataaacctgc ccaacaggca taaccgtcgc aggcgcaatt
    10441 tccattgcat ccatctctac cgaggcaaca ggctggtaag caggagcagg cacgtgctca
    10501 tccagatcaa aaagctgtac acgcccgccc gtgttctcat aaacaaccga tgacaaatca
    10561 acaaatcgat ccgagtattc aaccacttcc tcagattgag gcatttctaa aatcggcctc
    10621 cccggttccg tgttgaaata tgaacaccct accagtgcaa aactggcaca agatatgcaa
    10681 agaaaacggg aaagataaaa gcgatgctgc atcataaatt taatcagtaa actctaaact
    10741 taaacaatga aatcatgatc agaaatttct cgccctttta caagcccgct gaacccaaaa
    10801 gctattttta cccccggtat tttcgcggcg cccctcgcga atcatgcagc atttttttat
    10861 cgcttttcag cattatttcc gctatattgc cctttatttt tgctgcgttc tcatccactt
    10921 aatcaaaaat ggttagacaa tggttcaggt cgattacaac atgtccaaaa ataaaaacgc
    10981 acaaaaagcg ccccaaaacg acaacaatgc cgcagcgcga aaagcacttc agcgacagat
    11041 cgaaacgctc cccgatcccg tcgccctgtc gcaagccctg atgaatgctt accagcaatc
    11101 ccagccgctc ttcatggata tgctgcaaaa aatggggcaa acacccccct cggcagaact
    11161 tccctctttt gatcccgacc cgatgaatgt tggaagcgcc ggcgcacacc ttataaacgc
    11221 aatggccaca gacccactaa aattctggga tttgcaaata acctatgcac aaaaacaagc
    11281 ccagctctgg caatccagca tgaacaaaat gtggggacaa gaaagcaaac ccgtaattaa
    11341 accgcaaaaa agcgatcgcc gcttccgtga tgacagttgg aatgacagcg tcttcttcga
    11401 ctttataaaa caatactacc tgctcgcttg caattttgta gaacaaaata tcgaaaacgc
    11461 ggaaggcctc tcagaaaacg aaaaaagaaa actcaccttc accagcaaac tgatgatgga
    11521 tgccctctct ccctcaaact tcgccatgac aaaccccgaa gtcctaaaag aaaccttgca
    11581 aaccggtggc atgaatctgg tccacggctt acaaaacatg caggaagact ttgcacgcgg
    11641 caatgggcaa ctcaaaatca gcaccaccga ttacgacgcc tttaagc
//
LOCUS       DAOSWR010000061        11586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3526_length_11586_cov_6.052988, whole genome shotgun sequence.
ACCESSION   DAOSWR010000061 DAOSWR010000000
VERSION     DAOSWR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_11586_cov_6.052988"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK513_05860"
     CDS             <1..480
                     /locus_tag="PK513_05860"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOO82007.1"
                     /translation="NMKNAWKSIYGLNLALMIIYGTLGLIIGFSTLTLVYLPVLIITS
                     WIGGWLFYIQHQFENAHWERQEQWSYNQAALMGSSYYDLPPIIQWFTGNIGLHHIHHL
                     NATIPNYKLQSCLDAHPDLKHLNHMNFKDSLKSIKLALWDETRRKMISFEEMDKQAA"
     gene            607..1596
                     /locus_tag="PK513_05865"
     CDS             607..1596
                     /locus_tag="PK513_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82008.1"
                     /translation="MNRLRNANILVVNEIGSTRKMLTNILRILGAGSIDEASDKADAF
                     RKFNEKDYTLVISALIDGSQDAIGLAKDIRNNPASRNRIVPIIATTGPNTLHLVDAAR
                     EVGITDLLQTPFTVDDMSRHLNYNLSLQQEQLEAAALPQEKPQIDTTKSNDPEGGWPE
                     EEEATSLTKMLLEHYMRHHEIVFMKLKFAQDATQKSIHQLRSTHEKVRHRDNTNIHEF
                     NDFEKMWEEIIQIFLNGGLAEEELFKIEKLVTTIPEDIKKHYNTLSAQDKSFLSLVES
                     LNADAYKKAKNRVTALQKQPNPLHGKTPEEYTTPKETEEENPDAFIFMPKRRK"
     gene            complement(1608..2114)
                     /locus_tag="PK513_05870"
     CDS             complement(1608..2114)
                     /locus_tag="PK513_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82009.1"
                     /translation="MNKNNDKVEIFHRVNKLKSKAGLSAFAKGQGQIQNFRIQKAQKV
                     IDEKEAEYSVEVENVLRNLQVSWDACVGAEKSDVRADALERVYNYSNNAKDLASMYGY
                     DLMGYFALSLRDFCEKIDIHKEEHQIIVRAHINAMLVTLHERLRSDESDKAKELMNSV
                     ALAIQKYS"
     gene            complement(2101..2652)
                     /locus_tag="PK513_05875"
     CDS             complement(2101..2652)
                     /locus_tag="PK513_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82010.1"
                     /translation="MTADEVLDLSKFTILIVDDYSFVSQIIAATLKEMGIGRVLTSEN
                     GAQAKEQIQALNNFENGSNIDVVIMDWLMPVMDGRALLQWIRQSNKETIKFLPVVVCS
                     AYTSGSLVSETRDLGANEVIVKPVSAAELAKRIQRTINSPRPFVKSKSFFGPDRRRQN
                     RAFDHEDRRKAKPQDLKAHYEQK"
     gene            complement(2755..4053)
                     /locus_tag="PK513_05880"
     CDS             complement(2755..4053)
                     /locus_tag="PK513_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO82011.1"
                     /translation="MKNLSSLSKAQICLGLTLLCMLGGYFSSLYLVALAIGIITLATG
                     FYFIQNAQESITCATDACKKLGHGDFETRLTNIAEDGEISEFLWSVNEMTDFMDAFVR
                     ESTAAMEYVSRNQYFRRIIEDGMHGNLLNGARVINQATLNVADKMESFVGIADDVDNS
                     LKQVVSQINSTVETLESGAQTMGDTVASTRQGAETVVLSSNEMSMNVQTISAAAEEMS
                     SSIAEISTQITKTSTMASEAVHDADQAREIINALATTAASINEVITLIEDIAEQTNLL
                     ALNATIEAARAGEAGKGFAIVASEVKNLAAETAKATDDIRQQVNEVQGATNSAVSAFD
                     GIGKSIADINEACTVVAAAVEEQNAASLEIASNAQKASTATNTVAENIQEIGADITKV
                     DEISAQVVDATEQLSLHSKDQVEALLNKMGVFMGELKKIA"
     gene            complement(4071..4607)
                     /locus_tag="PK513_05885"
     CDS             complement(4071..4607)
                     /locus_tag="PK513_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO82012.1"
                     /translation="MKNSHVTGVERTFDKNEIIVSKTDLKGRITYVNRVFMTLADYTE
                     KDLLDQPHSIIRHPEMPRCVFKLLWDTIEKDKKEIFAYVINRSANGDHYWVLAHVTPS
                     FDEGGNIVGYHSNRRVPDRRVLNDTIVPLYKKLLDEENSHKNAKEGMNSAYQMLVALL
                     EEKGVGYDELIFSLSNAA"
     gene            complement(4651..4827)
                     /gene="ccoS"
                     /locus_tag="PK513_05890"
     CDS             complement(4651..4827)
                     /gene="ccoS"
                     /locus_tag="PK513_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HOO82013.1"
                     /translation="MSILIYLIPIALFLGLLGLAAFVWSLKSGQYDDLDGAAHRILLD
                     DEKTLTQEHDIRHS"
     gene            complement(4824..7049)
                     /locus_tag="PK513_05895"
     CDS             complement(4824..7049)
                     /locus_tag="PK513_05895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO82014.1"
                     /translation="MGRSALSNNAQLHSPVNGNHDTQTLSGYETLVEKTPEGLNTISV
                     LVSGVYCAACIQKIEGSLSQEQDIILARLNFTTRRLTLTWSGVPEQSNRYIHIIENLG
                     YTVNPFDPQIWQEESKAEERFLLLCLGVAGFAMGNIMLLSVGLWSATGETMGLATREF
                     LHLISAAIALPTILFSGRPFFRSAIKALRGRHANMDVPISLALILASSMSLFETINGG
                     EHVYFDSAVMLTFFLLIGRTLDFRARKQARAGASDLLAVMTGFASVIEDGKVRRVLIR
                     DICEGNHVRIAAGENFPVDGQIIEGQSEVDTSLVTGETLPRAVKPGNDIFAGTLNLSA
                     SVTMIVTKPAKDSLLSEIVKLMEQAEQSKARYVRLADRAARLYTPVVHMLAALAFLGW
                     FFIGGLDWQAALMISITVLIITCPCALGLAVPVVQVLATGKLMKNGVLIKSGDALERL
                     ASIDTVLLDKTGTLTLGHPALEGGLPQEALRLAASLAAHSRHPLSKAIGQSYAGTLLE
                     TKHIQEHSGQGLEGVIDGKSVRLGSRTWCGDKNAPPSDMLELWLRIDNQTPIAFFFTD
                     HLREDACDCIEALKKSGLTPIIVSGDRNHITQKSAKECGIERFYGEQTPPQKFQIMEN
                     LKREGHKILMVGDGLNDAPVLTGATVSMAPGTAIDMAQNAADIIFMGKKLMPVHTAYN
                     TARLAQKLVKQNFTLAVLYNIIAVPLALSGLVTPLVAAIAMSGSSLIVIANSFRLKVI
                     K"
     gene            complement(7001..7468)
                     /locus_tag="PK513_05900"
     CDS             complement(7001..7468)
                     /locus_tag="PK513_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOO82015.1"
                     /translation="METAIKNPKDKWIPWYFVAFFAVIAILDGIFVYVAISTHTGVTT
                     ENAYEKGLAFNENFEKFRAQPKLQEQLSFENGVLHWNVKDEAGRPLGNARVIAKIIRP
                     VQDGYDFEITLDYMRRGVYEAKLDLPLKGLWKAKLNCTWDDQHYQTTHSFTAP"
     gene            complement(7471..8868)
                     /gene="ccoG"
                     /locus_tag="PK513_05905"
     CDS             complement(7471..8868)
                     /gene="ccoG"
                     /locus_tag="PK513_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOO82016.1"
                     /translation="MLELFAKQEKIYPKRVWGKYRKMKWATMIATLGLYYLAPFIRWD
                     RGPNAPDQAIMIDLPARRAYWFWIEIWPQEVYLLTGILILAAIGLFVVTSMFGRVWCG
                     YFCPQTVWTDLFVWVERIIQGDRNARKRLHDSPWSFDKIGKILATHFIWLVIAWCTAG
                     SFVLYFNDAPTLVRSFFEFDVSPTVLGFIGGLTFSTYLMAGFAREQVCTYMCPYARFQ
                     SAMFDKDTLIIGYDEKRGEPRGSHKKGESWDGRGHCIDCTACVQVCPTGIDIRNGLQM
                     ECIACGLCVDACNNIMDKLDLPRGLIRYDTTNHMAAEIKGGKEKFHIMRMRTFYYGII
                     MAVVGSIMLYALINRAQLELHVLHDRSPLFVQLSSGEIRNGYTIKILNKTFENRTYTL
                     SVEGLPEAKIEVSAAGNIDSNTLYVDADSVGEYHIFISADIEPGPARDVTLTLTDSDG
                     SLHDSYTSTFITQRK"
     gene            complement(8970..9845)
                     /gene="ccoP"
                     /locus_tag="PK513_05910"
     CDS             complement(8970..9845)
                     /gene="ccoP"
                     /locus_tag="PK513_05910"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HOO82017.1"
                     /translation="MSDDNKKKEIDDLSGVETTGHEWDGLKELNNPLPRWWVWIFIVT
                     VIWSAWYFVVYPSWPVPGGATEGTSGYTQFKELAHNQAEITARQEAYLAQFEGADFET
                     ILKTPDLYAFAVAGGKSAFKDNCATCHGAGGQGAKGFPNLNDDDWLWGGSLEDIHQTI
                     QYGIRADNWDTRDSQMPAFGADELLERSEIDAVVDYVLTLSGGKQTSSHAQGMQIFAE
                     QCAACHGEDGRGMRELGAPNLTDKIWLYGGDRETVYETVFNARAGLMPAWNERLDENT
                     IKQLTIYLHELGGGE"
     gene            complement(9849..9995)
                     /locus_tag="PK513_05915"
     CDS             complement(9849..9995)
                     /locus_tag="PK513_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HOO82018.1"
                     /translation="MIDFITDHAGMIGLLFFFGFFVAVAMWTFRPGAKENYKKHAHIP
                     LEEN"
     gene            complement(9992..10729)
                     /gene="ccoO"
                     /locus_tag="PK513_05920"
     CDS             complement(9992..10729)
                     /gene="ccoO"
                     /locus_tag="PK513_05920"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HOO82019.1"
                     /translation="MSWKSHETLEKNSILLLIGIIVVVLIGGIVEIVPLFRKETVIEP
                     VEGVRPYTPLELAGYNIYIREGCYNCHSQQIRPLRDEVVRYGHYSLAAESMYDHPFQW
                     GSKRTGPDLARVGGKYSDEWQTAHLVEPRDLVPESIMPSYGFLLKKGAKLHSLDKHLK
                     VLRITGVPYSDEMIENAEKDAAAQATGEDRADISGLQERYGEKVNARDFDGQENMISE
                     MDALVAYLQVLGTMVDFEAAQQVEGAQ"
     gene            complement(10726..>11586)
                     /locus_tag="PK513_05925"
     CDS             complement(10726..>11586)
                     /locus_tag="PK513_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOO82020.1"
                     /translation="VQAGVQSAMIQWWYGHNAVGFFLTAGFLGMMYYFVPKQANRPIY
                     SYRLSIVHFWSLIFIYIWAGPHHLHYTSLPDWAQTLGMTFSIMLWMPSWGGMINGVMT
                     LSGAWHKLREDPVLQFMIVALAFYGMSTFEGPLMSIRAVNSLSHYTDWTIGHVHSGAL
                     GWVGFISFGALYYLVPRLWGRERLYSTKLVSMHLWIATIGIVFYIAAMWVSGIMQGLM
                     WRSYNDMGFLEYSFVESVMAMKPFYMIRVLGGLLYLTGALIMVYNFWKTIRGDVNEKE
                     QQLLSQGATA"
BASE COUNT         3104 a         2818 c         2707 g         2957 t
ORIGIN      
        1 aatatgaaaa atgcctggaa aagcatctat ggcctcaatc tcgccctgat gattatctat
       61 ggcacacttg gcctgataat cggcttttct accttaacac tggtttacct gccggtcctg
      121 atcatcacct cgtggattgg cggctggcta ttttatatcc agcaccaatt tgaaaacgcc
      181 cactgggaac gccaggaaca atggagctat aatcaagccg cattgatggg cagttcatac
      241 tacgatctgc ccccaatcat ccagtggttc accggcaata tcggcctcca ccatatccac
      301 catctcaacg ctaccatccc caattataag ctccagtcgt gtcttgacgc ccacccggat
      361 ctcaaacatc tcaaccacat gaacttcaaa gatagcctga aatcaatcaa gctggctctt
      421 tgggacgaaa cccggcgcaa aatgatcagc tttgaagaaa tggataaaca ggcggcataa
      481 cggtaccttg aaaaatacga aacacacaat tctgaaaacg cggtgtcaaa aaatcatttt
      541 ctaaaaccca aatcacccct ataatttgta cacaatcgta tttactcccc ggaaagccca
      601 tccataatga accgcctgag aaatgccaac atccttgtcg tcaatgaaat aggcagcacc
      661 cgtaagatgc ttaccaacat tttacgaata cttggagcag gctcaataga cgaagcaagc
      721 gataaagcag acgccttccg taaattcaac gaaaaagatt acacattggt aatatcagca
      781 ctgatcgacg gaagccaaga tgcaattggc ctcgcaaaag atattcgcaa caacccggcc
      841 tctcgaaacc ggatcgtccc aattatcgca acaaccggcc ccaatactct ccacctcgta
      901 gatgccgcgc gcgaagtcgg tataacagat ttactgcaaa cgcctttcac agttgacgat
      961 atgtcacggc acctcaatta caacttaagc ctgcaacaag aacagctgga agccgcagcg
     1021 cttccgcaag aaaaaccaca gatcgacacc acaaaatcaa acgatccgga aggcggttgg
     1081 ccagaagaag aagaagccac ctcgcttacg aaaatgttat tagaacatta catgagacac
     1141 catgaaatcg tctttatgaa attaaaattt gcacaagatg caacccaaaa aagcattcat
     1201 caactacgca gcacgcatga aaaagtgcgc caccgggata acacaaacat ccacgaattc
     1261 aatgatttcg aaaaaatgtg ggaagaaata atccagatct tcctcaatgg cggcttggca
     1321 gaagaggaac tcttcaaaat cgaaaaactt gttacgacaa ttcctgagga catcaaaaaa
     1381 cactacaata cgctcagcgc ccaggataaa agttttttaa gtctggtcga aagtctgaac
     1441 gcagacgcat acaaaaaggc caaaaaccgc gtaaccgctc tacaaaaaca acccaaccca
     1501 ctccacggga aaacacccga agaatatacc acgcccaaag aaacagaaga agaaaatccg
     1561 gatgctttta tttttatgcc caaacgccgg aaataagagc catctttcta agagtatttc
     1621 tgaatagcca acgccacact gttcatcagt tctttagctt tgtcactctc gtccgaacgc
     1681 agcctttcat gaagagtcac aagcatcgca ttaatatgcg cccgcactat aatttgatgt
     1741 tcttccttat ggatatcgat tttttcacaa aaatcacgca aagacaacgc aaaatagccc
     1801 atcaggtcgt acccgtacat agaggctaaa tctttcgcat tattggaata attataaacc
     1861 ctttccaatg catccgcccg tacatcagac ttttctgcac ccacacacgc atcccaactt
     1921 acctgcaaat tccgcaaaac attttcaacc tccaccgaat attcagcttc tttctcatca
     1981 attacctttt gcgccttctg aatacgaaaa ttctgaatct gtccctgccc cttcgcaaaa
     2041 gcagacaagc ctgccttgga ctttaatttg ttaacacggt ggaaaatctc aactttatca
     2101 ttatttttgt tcataatgcg ccttcaaatc ttgtggttta gctttccgcc ggtcttcatg
     2161 atcaaaggcc ctgttttgcc gacgccggtc aggcccaaaa aaactcttac tttttacaaa
     2221 gggtcgcggg ctgttaatgg tacgctgaat acgtttagcc aattccgcag cagaaacagg
     2281 tttaacaata acctcattag caccaagatc gcgcgtttcg ctcacaagcg atccagacgt
     2341 ataagcacta caaacaacca ccggcaaaaa tttaatggtt tccttattgc tctgcctgat
     2401 ccactgcaaa agcgccctgc catccataac cggcatcagc caatccataa tcacaacatc
     2461 aatattgctg ccattctcaa aattattaag cgcctgaatt tgttctttcg cctgcgcccc
     2521 attctcagac gtcagaacgc ggccaatgcc catctctttt aaggttgccg ctataatctg
     2581 agagacaaaa gaatagtcat cgacaataag aatcgtaaac ttgctaagat ctaatacttc
     2641 atcggctgtc ataagcttta tgatcaaatc actaaaaaat tataaaaaga cttatttttc
     2701 aataacatat ggtgaacgct atggattaaa atctggcttc cactccctta aggcttaagc
     2761 aattttcttc agttcaccca tgaacacgcc cattttattg agtagggctt caacctgatc
     2821 tttgctgtgc aaggatagtt gctcggttgc atctacgacc tgagcgctga tttcatccac
     2881 tttcgtgatg tccgcaccga tctcctgaat gttctcagca acagtattgg tagcggttga
     2941 ggctttttgt gcgttggaag caatttcaag ggaggccgcg ttttgttctt cgacggcggc
     3001 agcgacaacc gtgcaggctt cattgatgtc ggcaatagat ttccctatgc catcaaacgc
     3061 ggatacggcg ctgttggttg caccttgaac ttcgttgact tgctggcgga tatcgtcagt
     3121 tgcttttgct gtttcagccg caagattttt gacttctgag gcaacaatgg caaaaccttt
     3181 tccagcttca ccagcacgtg ctgcctcaat tgttgcgtta agggcaagaa gatttgtttg
     3241 ttcggcaata tcttcaatca gcgtaataac ctcgttgatg ctagcagcgg tagtagccag
     3301 tgcgttaatg atttcgcgcg cctgatcggc atcatgaacg gcttcgctgg ccattgtcga
     3361 agtttttgta atctgtgtgc tgatttccgc aatggaagaa gacatctctt cggcagcagc
     3421 cgaaatggtc tgtacattca tcgacatttc attggaactg aggacaaccg tttctgcacc
     3481 ctgacgggta gaggctacgg tatctcccat tgtctgagct ccactctcaa gggtttccac
     3541 ggtgctgttg atctgactga caacttgctt gagagagttg tcaacatcgt cagcaatgcc
     3601 gacgaaagat tccatcttat cggccacatt gagggtggcc tgattgataa cgcgggcgcc
     3661 atttaataaa ttcccatgca taccgtcttc gataatgcgg cgaaaatact ggttacggct
     3721 gacatattcc atagcagcag tggattcacg aacaaaggca tccataaagt cggtcatttc
     3781 attgacagac cataaaaatt cagagatttc accatcttcc gcgatattgg tcaggcgtgt
     3841 ttcaaagtca ccgtggccga gttttttgca agcgtcagtt gcacaggtga ttgactcctg
     3901 cgcattttga atgaaataaa agccggtcgc cagtgtaatg ataccaatcg ccagcgcaac
     3961 cagataaaga gatgaaaaat atcctccaag catacataac agcgttagcc ccagacagat
     4021 ttgagcttta gaaagagagg atagattttt catagcaaat tcctttttct tcaggctgca
     4081 tttgaaagag aaaagatgag ctcgtcgtaa ccaacgcctt tctcttcaag taaagccaca
     4141 agcatttgat aggcgctgtt catgccttct ttagcatttt tgtggctgtt ttcttcatct
     4201 aatagctttt tatatagcgg aacgattgta tcgttgagga cgcgtctgtc tggaacgcgt
     4261 cgattagagt gatatcctac gatattgccc ccttcatcga agctgggggt gacatgggca
     4321 agaacccagt aatgatcgcc gttggccgaa cggtttataa cgtaggcaaa tatctctttt
     4381 ttatcttttt ctatcgtatc ccacagcaac ttaaagacgc agcgtggcat ttcaggatgg
     4441 cgtatgatgc tgtggggctg atcaaggaga tctttttcgg tatagtccgc tagcgtcata
     4501 aagacgcggt ttacataggt tatgcggcct ttgagatccg ttttactgac aatgatttca
     4561 tttttatcga aggtgcgttc tacacctgta acgtgtgagt tcttcatatg ttttatctat
     4621 tctagattaa aattagagct ttaactctgt ttaactgtga cggatatcat gctcttgtgt
     4681 caatgttttt tcgtcatcga gcaaaatgcg atgagctgcg ccgtcgagat cgtcatactg
     4741 acctgatttc aaactccaga caaaggcagc caggccaagc aggccaagaa acagggcaat
     4801 ggggatgagg tagataagga tgctcatttt atgaccttta agcgaaatga attggcgatc
     4861 actatgagtg acgaacctga catggcaatc gcagcaacga gcggcgtaac aagtccgctc
     4921 agtgccagcg gtacggctat gatattataa aggacagcta aagtgaaatt ttgttttacg
     4981 agcttttgag caagccgagc ggtattatag gctgtatgca caggcatgag ttttttgccc
     5041 atgaaaataa tatcggctgc gttttgtgcc atatcgatgg cggtgcctgg cgccatcgag
     5101 acggttgccc cggtcaggac aggcgcatca ttcaggccat cgccgaccat aaggatttta
     5161 tgaccttcgc gtttaaggtt ttccatgatt tggaattttt gcggtggcgt ttgttcgccg
     5221 taaaaacgtt ctataccgca ttctttggcg cttttttgtg ttatgtgatt tcgatcgccg
     5281 gagacaataa tgggtgtcag accggatttt tttagagcct caatacaatc gcatgcatct
     5341 tcgcgcaggt gatcagtgaa gaaaaaggca ataggtgtct ggttgtcgat ccgcaaccat
     5401 aattccagca tatctgaagg cggcgcattt ttatcaccgc accatgtccg gctgccaaga
     5461 cgaacggatt tcccatcaat aacaccttct agtccctgac cggaatgttc ctgtatgtgt
     5521 tttgtttcca ataaagttcc tgcgtaagac tggcctatag ctttggaaag tgggtggcga
     5581 ctgtgggcgg ctagagaggc ggcgaggcgc agggcttctt gcggcaggcc tccttccaat
     5641 gcggggtggc ccagtgtgag cgtgccggtt ttgtcgagca gaacggtgtc aatactggcg
     5701 agtcgctcta aagcatcgcc ggatttaatg agtacaccgt ttttcatgag cttgccggtg
     5761 gccaggactt gtacgacggg tacggccagt cccagtgcgc aaggacaggt gatgatgagt
     5821 acggtgatgg agatcatgag cgcggcttgc cagtctaagc cgccgataaa aaaccagcct
     5881 aaaaaggcga gcgcggccag catgtgcacg acgggtgtgt agaggcgcgc agcacggtca
     5941 gccagacgaa cgtagcgggc tttgctttgt tcggcctgtt ccatgagttt gacaatttcg
     6001 ctgagcaggg agtctttggc cggtttggtc acaatcattg taacggaggc ggacaggttt
     6061 aatgttcccg caaaaatatc gtttccaggc ttgacagcgc ggggcagggt ttcgccggtg
     6121 acaagcgagg tatcaacttc gctttggcct tcgatgatct ggccgtcgac ggggaaattt
     6181 tcccctgcag caatacggac gtggttgcct tcgcagatat cgcggatcag gacgcggcgg
     6241 actttgccgt cttctataac gctggcaaaa ccggtcatga cggccaaaag gtctgaggcc
     6301 cctgcgcggg cctgtttgcg ggcgcggaaa tcgagggtac ggccaatcag caggaagaac
     6361 gttagcatga ccgcagaatc gaaatagacg tgttcgccgc catttatggt ttcaaaaagg
     6421 ctcatggagc tggcaagaat aagggcgaga gatatgggca cgtccatgtt ggcgtgtctg
     6481 cctctaagcg cttttatggc agagcggaag aagggacggc cagaaaataa aattgtcggc
     6541 agggcgatag cggcagagat taaatgcagg aattcgcgcg tggccagtcc cattgtttcg
     6601 cctgtggcgc tccacagacc gacggaaagc agcataatgt tacccatcgc gaaacctgcc
     6661 acgccaaggc aaagcaacaa gaaacgctct tcagctttac tctcttcctg ccagatttgt
     6721 gggtcaaacg gattaacggt atatcccaga ttttcgatta tatgtatata gcgattgctt
     6781 tgttctggta cgccggacca ggtaagtgtg agacgcctgg ttgtaaaatt gagccgcgct
     6841 aaaattatat cctgttcttg ggaaagactg ccttcgatct tctggataca agcggcgcaa
     6901 taaacaccgc taacgagaac ggaaattgtg ttcaaccctt caggcgtttt ttcaacaagc
     6961 gtttcatagc cggataatgt ttgcgtatca tggtttccgt ttacggggct gtgaagctgt
     7021 gcgttgtttg atagtgctga tcgtcccatg tgcagttgag ctttgctttc caaagacctt
     7081 ttaggggcag gtcaagcttt gcttcataaa ccccgcgccg catgtagtct agcgttattt
     7141 caaaatcata gccatcttgg accgggcgga tgatttttgc aatgacgcgg gcatttccca
     7201 gtggcctacc cgcttcgtcc tttacgttcc agtgtaatac gccgttctca aaactcagtt
     7261 gctcttgcaa ttttggttgc gcacgaaatt tttcaaaatt ttcgttaaag gccaggcctt
     7321 tttcataggc attttcggta gtaacaccgg tgtgagtgct tatggcaaca tagacaaata
     7381 taccatcgag aatggcaata acggcgaaga aagctacgaa ataccatgga atccatttgt
     7441 cttttggatt tttaatggca gtttccatgg ctactttctc tgcgtgataa aggttgatgt
     7501 gtagctatca tgcaaggatc catctgaatc tgtcaatgtt agcgttacat cacgggcggg
     7561 accgggctca atatcggcac tgatgaagat gtgatattca ccaacgctgt cagcatcgac
     7621 atagagcgta tttgaatcga tatttcctgc tgcgctaact tcaatttttg cttcgggcag
     7681 gccttcaaca cttaaggtat aggtccggtt ttcaaaggtt ttgttgagaa tttttatggt
     7741 gtagccgtta cggatttcac cgctggaaag ctgaacaaat agcgggctgc gatcatgcag
     7801 gacgtggagt tccagctgtg cccggtttat aagcgcatag agcatgatgc tcccgaccac
     7861 agccatgata atgccataat agaatgtgcg catgcgcata atatgaaatt tttctttacc
     7921 gcctttgatt tctgcggcca tgtggttggt ggtgtcataa cggatcaggc cacgtggcaa
     7981 atcaagcttg tccatgatgt tgttacaggc atcgacgcac aggccacagg ctatgcactc
     8041 catttgcagt ccattacgga tatcgatgcc ggtggggcag acctgaacgc aagcggtgca
     8101 atcgatgcaa tgaccgcgcc catcccagct ttcacctttt ttatgactac cgcgaggttc
     8161 gccgcgtttt tcatcataac caatgatgag agtatcttta tcgaacatgg cagattggaa
     8221 acgggcgtag ggacacatat aagtgcaaac ctgttcacgg gcaaaaccgg ccataaggta
     8281 agtggagaag gtcagaccac cgataaagcc gagcacggta ggagagacat caaattcaaa
     8341 aaagctgcga accagtgtgg gcgcgtcatt gaaatacagc acaaaagatc cggctgtaca
     8401 ccaggcgata accagccaga tgaagtgggt agcgagaatt tttccaatct tgtcaaagct
     8461 ccagggactg tcgtgaagtc ttttgcgagc attgcgatcc ccctggatga tgcgttcaac
     8521 ccagacaaaa agatcggtcc agacggtttg tggacagaaa tagccgcacc acacacggcc
     8581 aaacatgctg gtgacaacaa acagaccgat ggccgcaaga ataagaatac cagtgaggag
     8641 atagacttcc tgtggccata tttcgatcca aaaccagtag gcgcgccggg cgggcagatc
     8701 aatcatgatg gcctgatcag gagcgtttgg cccccgatcc cagcgaatga aaggggcaag
     8761 gtagtatagc cccaacgtag caatcattgt cgcccatttc attttacggt attttcccca
     8821 aacacgtttg ggatagattt tttcctgttt ggcgaagagt tcgagcatgg gttttttcta
     8881 ttctaaaata tttttatata agtatatacg tatatacaca gcctaaaaaa agccttcttg
     8941 ggggaaggct ttttaaaatt tcctggatat tactcgccgc cgcccagttc atgaagatag
     9001 atcgtgagct gcttgatggt attttcatca agacgctcat tccaggctgg cattaatcct
     9061 gcgcgagcgt tgaagaccgt ttcatacact gtttcacgat ctccgccata gagccaaatc
     9121 ttgtcggtca ggtttggtgc gccgagttcg cgcattccgc gaccgtcttc gccgtggcaa
     9181 gccgcgcact gttcagcgaa aatctgcatg ccctgagcgt ggctgctcgt ctgtttacca
     9241 ccggaaagcg taagaacgta atcgacaaca gcatcaatct ccgaacgttc aagcagttcg
     9301 tcggcgccga aggccggcat ttgggaatcg cgagtatccc aattgtctgc acgaatacca
     9361 tactgaatcg tttggtgaat atcttcaaga ctgccgcccc agagccagtc atcgtcattg
     9421 aggttcggga agcctttagc accttgcccg cccgcaccgt gacaggtagc acaattatcc
     9481 ttgaaagcgg acttgccgcc ggcaacggcg aaagcgtaca gatcaggtgt tttaagaatg
     9541 gtttcaaaat ccgcaccttc gaattgagcc agataggcct cttgacgggc agtaatctct
     9601 gcttgattgt gcgcaagttc cttaaactgc gtatagccgg atgtaccttc agttgcaccg
     9661 ccagggaccg gccaggatgg atatacaaca aagtaccatg cagaccatat aacggtgaca
     9721 ataaatatcc atacccacca gcgcggtagc gggttgttaa gctcttttag accatcccat
     9781 tcatgtccag tggtttcaac gccggaaaga tcatcaattt cttttttctt gttgtcatcg
     9841 ctcatatttt agttctcctc aagcgggatg tgcgcgtgtt ttttatagtt ttctttggcg
     9901 ccggggcgaa aagtccacat tgccacggcg acaaaaaatc cgaagaaaaa gagtaggccg
     9961 atcataccgg catggtcggt gatgaaatct atcattgcgc cccctcaact tgctgggcgg
    10021 cttcaaaatc aaccatcgtg cccagaacct gaagataggc gacaagtgca tccatttctg
    10081 aaatcatatt ttcctgcccg tcaaaatcac gggcattgac tttttcgcca tagcgctctt
    10141 gtagcccgct tatatcagcg cgatcttcac cagtagcttg agcagcagca tctttttcag
    10201 cgttttcaat catttcatcg ctgtaaggaa caccggtgat acgcaaaact ttcaggtgtt
    10261 tgtccaggct gtgaagttta gcgccttttt tgagcagaaa cccatatgaa ggcatgattg
    10321 attccggaac aagatcacgc ggctctacca ggtgcgctgt ttgccattcg tccgaatatt
    10381 tgccgcccac acgcgccagg tccggcccgg tgcgtttgga accccattgg aatggatgat
    10441 cgtacatact ttctgcggcc agggaataat ggccatagcg cacaacttca tcacgcaggg
    10501 ggcggatttg ctgagagtgg cagttataac acccctcacg gatataaata ttatatccgg
    10561 caagttccag cggagtgtaa ggccgcacgc cttcgaccgg ttcaatcacc gtttccttgc
    10621 gaaacagcgg aacaatttca actattcccc cgatcagcac aacgactata atgccgatta
    10681 acaaaaggat ggagttcttt tcgagtgttt cgtggctttt ccagctcatg ctgttgctcc
    10741 ttgagagaga agttgttgtt ctttttcatt cacgtctccg cgaatggtct tccagaaatt
    10801 gtagaccatg atcagggcgc cggttagata gagcaggcca ccaagtacgc gaatcatata
    10861 gaagggtttc atggccataa cgctttcgac gaaggaatat tcgaggaatc ccatgtcgtt
    10921 atatgagcgc cacatcagac cttgcatgat accagacacc cacatggcgg cgatgtaaaa
    10981 tactataccg atagtagcga tccacagatg catggagacc agctttgtag aatatagacg
    11041 ttcacgtccc cataggcgcg gtacgagata ataaagtgcg ccgaagctga tgaagccgac
    11101 ccaccccaag gccccggaat gtacgtgacc gatggtccag tctgtgtagt gagacagaga
    11161 gtttaccgcg cggatggaca taagcggccc ttcgaacgtg ctcatgccat agaaggcgag
    11221 tgcaacgatc atgaattgca ggaccgggtc ttcacgtaat ttatgccaag cgccggacag
    11281 ggtcatgacc ccgttgatca tcccacccca ggaaggcatc cagagcatga tggagaaggt
    11341 catgcccaaa gtctgcgccc agtcaggcaa agatgtgtaa tgcaaatggt gcggccctgc
    11401 ccagatataa atgaaaatca gggaccagaa gtgtacgatc gacagacgat aggagtagat
    11461 cgggcgattc gcctgtttag gcacgaaata gtacatcata ccaaggaatc ctgccgtaag
    11521 gaagaaaccc accgcattgt gcccgtacca ccactggatc attgcgcttt gaacgccagc
    11581 ttggac
//
LOCUS       DAOSWR010000062        11418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3594_length_11418_cov_5.371117, whole genome shotgun sequence.
ACCESSION   DAOSWR010000062 DAOSWR010000000
VERSION     DAOSWR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_11418_cov_5.371117"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PK513_05930"
     CDS             <1..1104
                     /locus_tag="PK513_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82021.1"
                     /translation="RQPSLAGRFADVGIPERTQTQNIYVSPQTQQSIDQLDPKLLGVA
                     AEISGADNVIFVSDDDIRPEIEKIDAQRYGTSNILHGDDYAHYGEAFDAFRTGFKDAP
                     PIIVPKAAQHAYNEDASAQLYHHENGQSYCVITLPRTDDTADSYFRRVGGVKGFSKTP
                     DGELVKTQINIPESEVLDKAVVAEGILHEAAHCGLQHPDTERTMPRVLNEEQEADAVG
                     HEAVQYVFPDVGAESAQVTRDARAFGAIALDDPDHAGNMDLHSDPFVSPEDQVSGHRH
                     VHDAVVDKVAEMVSTDRHEARELLDSDPQLMHSTLDQMNREGVFLSNKAAQAYVDNYL
                     DVGQRRVPEYVGGQSDKEAVVPVPTPVVPSGPP"
     gene            complement(1109..2245)
                     /locus_tag="PK513_05935"
     CDS             complement(1109..2245)
                     /locus_tag="PK513_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO82022.1"
                     /translation="MDKNLDELKNMVVDVWTHGLFGVDIGSIIIALGILLSFLLIRGI
                     FSKFVLAKLHDWAAKSTTKLDDKIVDALIPPIKFIPVILGIFFASQYMGLSDDANELI
                     MRVERSLIAFTIFWALHRAADPLSHGLKKLEKLLTRAMMQWIFKTIKVLVVFVGAAVI
                     LEIWGIAVGPLLAGLGLFGAAVALGAQDLFKNLIGGITIIAEKRFHPGEWILVEGVVE
                     GTVEEIGFRSTKVRRFDKAPVHVPNSQLSDAVVTNFSRMTHRRIKWAIGVEYRTTVEQ
                     LQIIRDGIMNYIVENDAFARSDEISTFVRVDSFNASSIDFLVYCFTKTTDWGQWLEIK
                     EAFALEIKQIVEDKAGTGFAFPSQSIYVESLPGDAAEVFVPPKG"
     gene            2310..3011
                     /locus_tag="PK513_05940"
     CDS             2310..3011
                     /locus_tag="PK513_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOO82023.1"
                     /translation="MDLIAAKPSFEMLDYLLKRRSLKVGEFVEPGPDAEQLQTLLTAA
                     ARVPDHGKLCPFYFLVFEGDARGQAGDILAEIFKADNPDASEDKIEVERQRFLRSPLV
                     VGIVMRARPSKHPLWEQMMSAGSAAQNFVLAANALGFGVQWLSEWYAYDGRTCSAFGL
                     DERDVVAGFMHVGSPAAAAQERPRPDLEKIVTFWQRGCGLKKGDGYDLDKFEFPEDGF
                     DFSKLKRSAKAVQGL"
     gene            complement(3020..3529)
                     /locus_tag="PK513_05945"
     CDS             complement(3020..3529)
                     /locus_tag="PK513_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82024.1"
                     /translation="MFGSQYLQIKPEKVLQRLKDFELFCLGEKNLSPEMFKGLNEDLS
                     LSAILHIGKRHFRRDIDGNHLIWQGLDKREHDLGPIDDLKITQACPGYHKARTLALAG
                     DDVLGSDIVNIPTHKRQVVIVKMKDGTSGVGPNYKMALRNAALKMHLKYAFERTNRSD
                     IWKQYYGNC"
     gene            complement(3674..5380)
                     /locus_tag="PK513_05950"
     CDS             complement(3674..5380)
                     /locus_tag="PK513_05950"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO82025.1"
                     /translation="MTDIDKEIAPEIAQACRAWPFEEARQIIKRLRAMNRGEIKPGPK
                     GYVLFETGYGPSGLPHIGTFGEVARTTMVLRAFKRLAPEIPVKLTCFSDDMDGLRKVP
                     TNVPNPELLTKALGMPLSSVPNPFESDHLSFAEHNNDRLKEFLDSFGFEYEFLSSTAD
                     CYTNGRFNDALRIMLEKYNEVREAVLPILGAERTETYSPLFPIVQKKSADDHHPIILH
                     NALLREVIDADKGIATFEVLDDSEKERSGYSAGETITQSIFDGGCKAQWRADWALRWY
                     ALDVDYEMAGKDLVTAADIAAKIVQILGAEKNMMGPAGFRYELFLDEKGQKISKSKGN
                     GLSMEDWLTYAPHESLAYYMYQRPTTAKKLYFDIIPKAVDEYIQFVEKLPEQDAAKKL
                     ENPAWYIHDGAIPNAQHTPISFALLLNLVNAANTDDPATLWKFIQTAAPDATPESAPF
                     LDRLVDYAIRYYTDFILPEKQYRAPDARETAALGALATTLENMDSGLPAEEYMTEVFS
                     AGKNNGYEKDELRDWFKAIYEVCLGQSQGPRFGSFIKLYGVEDTVNLIRNALSGEFTK
                     AA"
     gene            complement(5475..5789)
                     /locus_tag="PK513_05955"
     CDS             complement(5475..5789)
                     /locus_tag="PK513_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82026.1"
                     /translation="MKAALKTCILGLAVLGLSACETGMGFYDDAPPYEIERTATHMQS
                     TPAPAPVAETPATPEPCSKPCMECDYSEWEARALQAEHDLRMCQEATDRVRDAYREEL
                     KK"
     gene            6469..6809
                     /locus_tag="PK513_05960"
                     /pseudo
     CDS             6469..6809
                     /locus_tag="PK513_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
     assembly_gap    6810..6909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6910..7274
                     /locus_tag="PK513_05965"
     CDS             <6910..7274
                     /locus_tag="PK513_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOO82027.1"
                     /translation="AKKPAAKKAVAKKAPAKKKPAAKKPAAKKAVAKKAPAKKPAAKK
                     AVAKKAPAKKKPAAKKKPAAKKKTVTKAVVRKAKVTKAPANSNAKAPVKRAASKAKTV
                     KKVAKKPAPKKKMTAKKK"
     gene            7606..9681
                     /gene="uvrB"
                     /locus_tag="PK513_05970"
     CDS             7606..9681
                     /gene="uvrB"
                     /locus_tag="PK513_05970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOO82028.1"
                     /translation="MIHNNDAPVQAQIEAGRAFDRSQPFRIHSDFTPSGDQPGAIKAL
                     VRGINDGLSDQVLLGVTGSGKTFTMAHIIEQTQRPALILAPNKTLAAQLYGEFKSFFP
                     DNAVEYFVSYYDYYQPEAYVPRSDTFIEKDSSINEQIDRMRHAATRALFERKDVIIVA
                     SVSCIYGIGSKEDYMAMAFGVEKGAALDRDEFIRKLIELQYIRNDIAFERGSFRVRGD
                     CVELFPAHFEDRAWRFDFFGDELETIVEFDPLTGEKFNALEGVRVFANSHYITPGPTM
                     AQAIKNIKADLKTRLSDLEADGKLLEAQRLDQRTQFDIEMLEATGMCQGIENYSRYLT
                     GRAPGEPPPTLIEYLPDNALMFLDESHVMLPQLRAMYNGDFGRKSTLVEYGFRLPAAV
                     DNRPLKFDEWNAMRHQTIFVSATPGPWELEQTGGEFVEQVVRPTGLIDPEVEIRPTTH
                     QVDDLMHEAKAVIAEGGRMLVTTLTKKMAEALTEYLNENGIAVRYIHSDVDTLERIEI
                     MQDLRKGVFDVLVGINLLREGLDIPECKRVMILDADKEGFLRSKTSLVQTVGRAARNV
                     EGKAILYADRITDSMQYAIDETARRREKQLEYNAEHGITPESVKKNLSSILDSVYEQG
                     DHVAVPLAADSAVQAFLHEHGDNLAKLRKHIEAMRKKMLEAAENLEFEEAARLRDEIK
                     QLEENELGL"
     gene            complement(9863..10006)
                     /locus_tag="PK513_05975"
     CDS             complement(9863..10006)
                     /locus_tag="PK513_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82029.1"
                     /translation="MNIVTAKEPRKPVQAGDYGKHAELPTKDEAPKPPQNTDGRKTLA
                     LWS"
     gene            complement(10006..10215)
                     /locus_tag="PK513_05980"
     CDS             complement(10006..10215)
                     /locus_tag="PK513_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOO82030.1"
                     /translation="MATGTVKWFNTNKGFGFIQPDDGGKDVFVHITAVERSAEISYLN
                     EGDKIEYDVQSEKGKESAQNLRKAA"
     gene            10683..11297
                     /locus_tag="PK513_05985"
     CDS             10683..11297
                     /locus_tag="PK513_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOO82031.1"
                     /translation="MRYLILAVLLIFSFPALAQDEGHTIRIIQEDGSVDVIDLRQSAS
                     EPEPKPAAQAPVEMPKAKAEPAPVKKAKAVPSKKSAPKKVVAVPLPAPKIVTPPPLPV
                     QRKVAPSPSTEEEGVKNAPVQKSEIRRAQAISVALDYAPPSSDVEVYRSEYNGQRVFA
                     VMFKIEDGYHEVLVDAHSGKVLESRKSDIMRAQPKPGHLPGSLQ"
BASE COUNT         2771 a         2414 c         3194 g         2939 t
ORIGIN      
        1 agacaaccca gtcttgcggg tcgttttgcc gatgtcggga ttcctgaacg aacacaaact
       61 caaaatatat atgtttctcc acaaacgcag caaagcatcg accagctcga tccaaaattg
      121 cttggtgtag ccgcagagat ttccggcgcg gataatgtga tttttgtttc ggatgatgat
      181 attcgtccgg aaattgagaa aattgatgct cagcgttacg gtacttctaa tattttgcat
      241 ggcgatgatt atgcgcatta cggagaggcc tttgatgcgt ttcgtaccgg atttaaagat
      301 gcgcccccaa tcatagtccc gaaggctgca cagcatgctt ataacgaaga tgcttcggcg
      361 cagctttatc atcatgagaa tggtcagtct tattgtgtga tcacattacc gcgtacggat
      421 gatactgcgg atagttattt tcggcgtgtg ggtggggtga agggctttag caagacacct
      481 gatggcgagt tggttaaaac acagatcaat attcctgaaa gcgaagttct tgataaggcg
      541 gtggtagcgg aaggcattct tcatgaagca gcacattgcg ggcttcaaca tcctgatact
      601 gagcgtacga tgcctagggt tttgaatgaa gaacaggagg cggatgctgt tggtcatgaa
      661 gctgttcaat atgtgtttcc tgatgttggc gctgaatctg cccaagtgac gcgagatgcc
      721 cgtgcatttg gggctattgc tttggatgat cctgatcatg cggggaatat ggatttgcat
      781 tctgatccgt ttgtttcgcc tgaagatcag gtctctgggc atcggcatgt tcatgatgct
      841 gtggtcgata aggttgcaga gatggtttcg acggatcgtc atgaagcgcg tgagctttta
      901 gactccgatc cccaattgat gcattcgaca ctggatcaga tgaatcggga aggtgtattt
      961 ttgagcaata aagcggctca agcctatgtt gataattatc tggatgtggg gcaacggcgt
     1021 gttcctgaat atgtgggggg gcagtctgat aaagaagctg ttgtgccagt gcctacacct
     1081 gttgtaccga gtggtccccc ttagaaattt accccttcgg agggacaaaa acttccgcgg
     1141 cgtcgccggg cagggattcc acataaattg attgtgacgg gaaggcgaag ccggtgccgg
     1201 ctttgtcctc aacgatttgt tttatttcaa gcgcgaaggc ttccttgatt tccagccact
     1261 gtccccaatc ggttgttttg gtgaagcaat agacgaggaa atcgattgag ctggcgttga
     1321 aactgtcgac gcgcacgaag gttgagattt catcggaccg ggcgaaagca tcgttttcta
     1381 cgatataatt catgatgcca tcgcggatga tctggagctg ctcaacggtt gtgcggtatt
     1441 cgacgccgat ggcccatttg atgcggcggt gggtcatgcg ggaaaagttg gtgactacgg
     1501 catcggaaag ctgtgaattt ggtacatgca ccggggcttt gtcaaagcgg cgcactttgg
     1561 tggagcgaaa accaatttct tcgaccgtgc cttcgacgac gccttcgacc aaaatccatt
     1621 cgccggggtg aaagcgtttt tcggcgataa tcgtgatgcc gccaatcagg tttttgaaca
     1681 ggtcctgcgc tcccaatgct acggctgcgc caaacaggcc gagaccggcg aggagcgggc
     1741 cgacggcgat gccccagatt tccagaatga cggcggcgcc gacgaacacg acgaggactt
     1801 taatcgtctt gaagatccat tgcatcatcg ctcgggtcag tagtttttca agctttttga
     1861 ggccgtggct taaagggtcg gcggcgcgat gcaatgccca gaagatggtg aaggcgatca
     1921 aggagcgttc aacccgcatg atcaattcgt ttgcatcgtc gctgaggccc atatactggc
     1981 tggcgaagaa tattccgaga ataacaggga tgaatttgat tggcgggatc agcgcatcga
     2041 cgattttatc atcgagcttg gtggtggatt ttgccgccca gtcatggagc ttggccagaa
     2101 cgaatttgct gaagatgccg cggatgagca aaaagctgag taagattccc aaggcgatga
     2161 tgattgagcc aatgtcaacg ccgaaaaggc cgtgtgtcca cacatcaacc accatgtttt
     2221 tgagttcgtc cagattttta tccattgatt ttgccgctcc tttggcaata ttgtcgttct
     2281 tcaatgtgta tgcaagccaa ggatatagca tggacctaat tgctgcgaaa cccagttttg
     2341 agatgctgga ttacttatta aaacgccgga gtttgaaggt gggtgaattt gtggagccgg
     2401 ggccggatgc ggagcaactg cagacattgt taacggctgc ggcgcgggtg ccggatcatg
     2461 gtaaattgtg tccgttttat tttctggtgt ttgaaggcga tgcgcggggg caggccgggg
     2521 atattctggc ggagattttt aaggcggata atccggatgc gagcgaagat aaaattgagg
     2581 ttgagcgcca gcggttttta cgttcgccgt tggtggtggg gattgtgatg cgcgcacggc
     2641 ctagcaagca tcctttgtgg gaacagatga tgagcgcagg ctctgcggcg cagaattttg
     2701 tgctggcggc taatgcgctg ggctttgggg tgcagtggct cagcgagtgg tatgcgtatg
     2761 atgggcggac gtgctctgcg tttggattgg atgaacgcga tgttgtggcc gggtttatgc
     2821 atgtgggcag cccggcagcg gcggcgcagg agcggcctcg ccctgatctg gaaaaaattg
     2881 taacattttg gcagcggggt tgcggcctta aaaaggggga cggttatgat ctcgataaat
     2941 tcgagtttcc cgaagacggg tttgattttt ctaaattaaa gcgttcggca aaagccgtgc
     3001 agggcttgta atcttcctcc taacaattgc cgtagtattg tttccaaata tcggatctat
     3061 tggttctttc aaaagcatat ttaagatgca tcttgagcgc agcattgcgc agagccattt
     3121 tgtaattggg gccgacgcct gatgtaccgt ctttcatttt aactatcacg acttgtcttt
     3181 tgtgggtggg gatgttgaca atatcgcttc ccagtacatc atcgccggcc agcgctagag
     3241 ttcgggcttt gtgataaccc ggacaggctt gggtaatttt taagtcatca atggggccta
     3301 aatcatgctc tcgtttgtcc agcccctgcc aaattaaatg gttgccatct atatctctac
     3361 ggaaatggcg tttgccgatg tgtaaaatcg cacttaagga aaggtcttcg ttgaggcctt
     3421 taaacatctc aggagaaagg tttttttctc caaggcagaa aagttcaaaa tctttaagcc
     3481 gctgaaggac tttttcaggt tttatttgaa gatattgaga accaaacatt tacacaccgt
     3541 aatttaagtc aaaaatctgt ttatagtttt atattaaatc cgaaatagtt aatttagttt
     3601 aaatttatgt aaaatttaga taaaatttta tgagtgtttt atgtaaggta aatttttatt
     3661 tgtttttaat agcttaagcg gcttttgtaa attctccgct taaggcgttt cgaattaaat
     3721 ttaccgtatc ttctacaccg tagagcttaa tgaaagaacc aaatctgggg ccttgagact
     3781 ggccgagaca gacttcatat atggctttga accagtcgcg gagttcgtct ttttcatagc
     3841 cgttgttttt accggcgctg aaaacttcgg tcatatattc ttcggcgggc agaccagagt
     3901 ccatattctc caacgtggtg gcgagcgctc cgagcgcagc ggtttcgcgg gcatcagggg
     3961 cgcggtattg tttttcgggc aggatgaagt ctgtgtaata gcggatagca taatcgacga
     4021 ggcgatctaa aaacggtgcg ctttcagggg tggcatcggg cgcggcggtt tgaatgaatt
     4081 tccacagcgt ggccggatca tcggtatttg cggcatttac caagttgagc aggagcgcaa
     4141 atgagatcgg cgtgtgctgg gcgttcggaa tggcgccatc gtggatatac caggccgggt
     4201 tttccagttt tttggccgca tcttgttcgg gcagtttttc tacgaactgg atatattcat
     4261 cgacagcttt ggggataata tcaaaataga gttttttggc cgtggtcggg cgttgataca
     4321 tataataggc gaggctttca tgaggggcgt aggtgagcca gtcttccatg gacaggccgt
     4381 tgcctttgga tttggaaatt ttttgacctt tttcgtcgag gaacagttcg tatctgaaac
     4441 cggcggggcc catcatgttt ttctcggcgc cgagaatctg gacgatcttg gcggcaatat
     4501 ctgcggcggt gacaaggtct ttaccggcca tttcgtaatc gacatccaat gcataccaac
     4561 gcaatgccca gtctgcacgc cattgcgctt tacagcctcc gtcaaagatg ctttgcgtga
     4621 ttgtttctcc ggcgctgtag cctgagcgtt ctttttcact gtcgtcgaga acttcgaagg
     4681 tagcgatgcc tttatcggca tcaatgactt cgcgcaggag ggcgttgtgc aggatgatcg
     4741 ggtggtgatc atcggcggat ttcttttgta caatcgggaa gaggggggaa taggtttcgg
     4801 tgcgttcggc tcccaagatt ggcagtacgg cttcgcggac ctcattgtat ttttcgagca
     4861 tgatgcgtag ggcatcgtta aagcgaccgt tggtataaca atctgcggtg gagcttaaga
     4921 attcgtattc aaagccaaag ctgtccagaa attctttcag gcggtcattg ttatgctcgg
     4981 cgaagcttaa gtgatcgctt tcgaacgggt tgggcactga tgagagtggc ataccgaggg
     5041 ctttggtcag aagttcgggg ttggggacat ttgttgggac tttgcgcagt ccgtccatgt
     5101 cgtcggaaaa acaggtcagt ttaaccggaa tttcgggggc gaggcgttta aaagcgcgca
     5161 ggaccatggt tgttcgggcg acctcgccaa aagtgcctat atgcggcaga cctgaggggc
     5221 cgtagccggt ttcaaacagt acatagcctt tggggccggg tttgatttca ccacggttca
     5281 tggcgcgcag gcgtttgata atctgccgcg cttcttcgaa cggccaggcg cggcatgctt
     5341 gtgcaatttc tggggcaatt tctttgtcta tatcagtcat ggctttacgc ttatcaaatt
     5401 cttttgcatt ttcaaatata taaaaagccg ggccacataa tcgtgcccgg ctttttaaaa
     5461 tccggttaaa attgttattt ctttagttct tcgcgatatg cgtcacgaac gcgatcggtg
     5521 gcttcctggc acattctcag gtcatgttcg gcctgaaggg ctcgcgcttc ccattctgag
     5581 tagtcgcatt ccatgcatgg ttttgagcat ggctctggcg ttgcaggtgt ttcggcaacc
     5641 ggagcaggag caggcgtact ttgcatgtgg gtagcggtgc gttcgatttc gtaaggcggg
     5701 gcatcatcat aaaaccccat acctgtttcg caagcagaca agcccaaaac ggccagtccc
     5761 aatatacatg tttttaaagc agctttcata ggctttgtcc tttgttgatt atctggaata
     5821 tcgcgagctt agcaagaatt aataagaatg caaagcgtct tgatgaaagc tacaagtttt
     5881 gattttggtt gcggcgcttt ttgtacgcgc ttcgtttata gagggtgagt aatataataa
     5941 tatttatgca tgcgacattg acgctgttga cgaggctgaa ttttatgtcg gctaaatcaa
     6001 aaacactata aagtaaactt acaattgcag tgtaattcca aatcatccat gaggtgattg
     6061 agatttcgcg gcaatcagtt tcgcttttcc agagtttgga gatttgcggc atgtatccaa
     6121 acaaaacaac aattccgact gtggaataaa gaagtccgat aatattcatt ttgggtcttt
     6181 cgatttgatc gtaatttact ttacatgcgt gatgacggtg tgcaaaaagt ttgttgaaat
     6241 tcatcatcaa aaataaaaaa tcgggtttgt ttttttgcga ttatttttta ggcaaaaggc
     6301 gtgtgaaaaa aaacctttgg agaataaggc gattcacagt atatttatcg gaagagtaac
     6361 tacttttgtc caatttattt cacccttcct ttcggtcagt tctgaccact tgcaaaacat
     6421 ttcaggagaa aactcatggc aaccacgaaa aagaaaccgg cggctaagaa acctgctgcc
     6481 aaaaagacga cggctaaaaa gcctgcggct aagacttcaa caaccaaagc agctgctgcg
     6541 aaaaagcctg tggctaaaaa agctccggcc aaaaaagctc cggccaaaaa agcaactgtg
     6601 aaaaagacag ctgctaagaa gcctgcggct aacactaata aaaaagcagc gcctaagaag
     6661 ccagcagcta agaaagctgt agcaaagaag gctccggcca agaagccagc ggccaagaag
     6721 ccagcggcta agaaagctgt agcaaagaag gctccggcca agaagccagc ggctaagaaa
     6781 gctgtagcaa agaaggctcc ggccaagaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnc ggccaagaag ccagcggcta agaaagctgt agcaaagaag gctccggcca
     6961 agaaaaagcc tgcagcaaag aagcctgcag ctaagaaagc tgtagccaag aaggctccag
     7021 ccaagaagcc agcggctaag aaagctgtag ccaagaaggc tccggccaag aaaaagcctg
     7081 cagctaaaaa gaaacctgcc gctaagaaga agactgtgac aaaagcggtc gttcgtaagg
     7141 caaaggtgac aaaagcgcct gcaaacagca atgctaaagc gccggtcaaa cgtgcggcca
     7201 gcaaagcgaa aacggtgaag aaagtcgcca agaagcctgc gcctaagaaa aaaatgacgg
     7261 caaagaagaa gtagacttta acgtcttata aatgcaaagc gccgctgagg atatccttgg
     7321 cggcgttttt tttgatacgt gaaaaatctt gtattgggac gggaagactc agtgtatatt
     7381 gatgatgttg tcggaattgc tctgacaatc gctgctgctg cttaaggcag ttatattgag
     7441 aggatacaag atcatggcga caaaacctgg atctaaaggc aaacccactc caaagaaata
     7501 gtgcgtttgt gctctctttt aagaataaag gcgcggggct gatcggtccc gcgttttctt
     7561 ttggcctctt cgcggttttg gcctatataa acccttatga aagcgatgat tcacaacaac
     7621 gatgcgccgg tgcaggcgca aattgaggcg ggaagggctt ttgatcgctc acagccgttc
     7681 aggatccatt ccgactttac cccgtcgggg gaccagccgg gggcgatcaa agcgctggtg
     7741 agagggatta atgacgggct ttccgatcag gtgctgctgg gggtgaccgg ctcgggcaag
     7801 accttcacca tggcgcatat tattgagcag acgcagcgcc cggcgctgat ccttgcgccg
     7861 aacaagacgc tggcggcgca gctttacggc gaattcaaaa gtttttttcc cgataacgcg
     7921 gtggaatatt tcgtgtctta ttacgattat taccagcctg aggcctatgt gccgcgcagc
     7981 gatacgttca tcgagaaaga cagctcgatt aacgagcaga tcgaccggat gcgccatgcg
     8041 gcgacgcgtg cgctgttcga gcgcaaggat gtgattatcg tcgcctcggt ttcctgcatt
     8101 tacggaattg gctcgaaaga agattacatg gcgatggcgt tcggcgtgga aaagggcgct
     8161 gcgctagacc gcgatgaatt catccgcaag ttgatcgagc tgcaatatat tcgcaacgac
     8221 attgcatttg agcgcgggag tttccgtgtg cgcggggatt gcgtcgagct gtttcctgcg
     8281 cacttcgaag accgggcgtg gcggtttgat ttcttcggcg atgagctgga aacgattgtc
     8341 gagttcgatc cgctcacggg tgagaagttc aacgcgctgg aaggcgtgcg ggtgtttgcg
     8401 aactcgcact atattacgcc ggggccgacg atggcccaag cgattaaaaa cattaaggcc
     8461 gatttgaaaa cccggctgtc ggatcttgag gccgatggaa agctgcttga ggcgcaacgt
     8521 ctggaccaac gcacgcagtt tgatattgaa atgctggagg ctacgggcat gtgtcagggg
     8581 atcgagaatt attcccgcta tctcacgggc cgggcaccgg gtgagccgcc gccaacgctg
     8641 attgaatatc tgccggataa tgcgctgatg tttctggatg aaagccatgt gatgttgccg
     8701 caattgcggg cgatgtataa cggggacttt gggcgtaaat ctacgctggt ggaatacggg
     8761 tttcggttgc cggcagcggt ggataaccgt ccgctgaaat ttgatgagtg gaatgcgatg
     8821 cgtcatcaga ctatttttgt ctctgccacg ccgggtccgt gggagctgga gcaaacgggg
     8881 ggtgaatttg ttgagcaggt cgtgcgccca acgggattga tcgaccctga ggtggaaatc
     8941 cgtccgacta cccatcaggt tgacgacctc atgcatgagg ccaaggcggt gatcgccgaa
     9001 ggcgggcgga tgctggtgac gacgctgacc aagaagatgg ccgaggcgct gactgaatat
     9061 ttaaacgaga acggtattgc cgtgcgctat atccattcgg atgtcgatac gctggagcgc
     9121 atcgagatta tgcaggattt gcgcaaaggc gtgtttgacg tgctggtcgg gattaacctg
     9181 ctgcgtgagg ggctggatat tcctgaatgc aagcgggtga tgattttgga tgctgataag
     9241 gagggctttc tgcgttcgaa aacctcgctg gtgcagacgg tggggcgcgc ggcgcgcaat
     9301 gttgaaggca aggcgatttt gtatgctgat cgcatcacgg atagtatgca atatgcgatt
     9361 gatgaaaccg cgcgccggcg tgagaagcag ctcgaatata acgcagagca cggcattacg
     9421 ccggaaagcg tgaaaaagaa tttatccagc attctcgaca gtgtgtatga gcagggtgat
     9481 catgtcgctg tgccgctggc cgcggacagt gcggtgcagg cgtttttgca tgagcatggt
     9541 gataacctgg cgaagctgcg caagcatatt gaggcaatgc gcaaaaagat gctggaggcc
     9601 gccgagaact tggaatttga agaagctgca aggttgaggg acgagattaa gcagttggaa
     9661 gagaacgaat taggtttata gtgagcgaat ggctaaagat cacattttat gcttttggta
     9721 accgtgtttg ttccttttag atttgagatt gataaaaaac atttggaaga aaatgctagc
     9781 taaactggtt ttttctaggc tgcatcattt tataaaaaac ggccatcttg tgacggccgt
     9841 ttctcaattc ggtaattacg ctttaactcc aaagggcgag ggtcttgcga ccgtcggtat
     9901 tttgtggtgg cttaggggct tcgtccttgg tcggcagttc ggcgtgttta ccgtagtcgc
     9961 cggcttgaac gggtttgcga ggttctttcg ctgtgacaat attcattaag cggccttgcg
    10021 caaattctgt gcagactctt tgcctttttc gctttgtacg tcgtattcga tcttgtcgcc
    10081 ttcgttcagg tagctgatct cggctgagcg ctcaacagcg gtgatgtgga cgaatacgtc
    10141 cttgccgcca tcgtcaggtt ggataaagcc gaagcctttg ttggtgttaa accattttac
    10201 tgttccggtt gccatgatat tttttccttt agcaaatcgg gttgaagccc tcgtccacct
    10261 tagtcttggg cttgtgtctg atcaggcgga aaggcgaaaa aatgccttaa ggcccgaaac
    10321 gtcaatcaaa cagattttct aaaaggggag gttttaacca gtgccgctgt tgaaaggcgg
    10381 cgtctcttaa aagtcgtgta tcaagtcgtc gtatagtcaa acgtgatggc attaagcgca
    10441 ggtcttggcg catatgtcaa acttattcga aggcttgaca aaatatgaat aaattcctat
    10501 aatatggctt ttagccgtag taaaaggagt gagcgcatgg cggtataatg agaagattgg
    10561 cgtgtttatc acgcgagcgc tgggcggaga agcaaaagct gctggagaag ctggcagagg
    10621 aaaaagcaga ctgaaaaaga gggttcactt cctttggccg aggaatcgat tatactggcg
    10681 ctatgcgata tttgattttg gcagttttgc tgatattttc ttttccggcg ttggcgcagg
    10741 atgaaggtca tacgatccgg attattcagg aagatggcag tgtcgatgtg attgatttgc
    10801 ggcaatctgc gtcggaacca gagccgaaac cggcggcgca agcgcctgtt gaaatgccca
    10861 aggctaaagc ggagccagct ccggtgaaaa aagccaaggc ggtgccttct aaaaaatctg
    10921 cgccgaaaaa agttgtggct gtgcctctgc ctgcgccaaa aattgtgacg ccgccgccgt
    10981 tgccggtgca gcgcaaagtt gcgccttcgc cttcaacgga ggaagagggg gtgaagaatg
    11041 ctccggttca aaaatctgag attcgccgtg cacaggcgat ttctgtggca ctggattatg
    11101 cgccgccgtc cagtgatgtg gaggtttatc gctctgaata taacggtcag cgcgtgtttg
    11161 cggttatgtt taagattgag gatggctatc acgaggtgct cgtcgatgcg catagcggca
    11221 aggttctgga aagccgcaag agtgatatta tgcgcgcaca gccaaagccg gggcatttgc
    11281 ccggctcttt gcaataatcc aaatattaat ctttttcagg tttaattcct cgccgcttac
    11341 ggcgtaatcg tcatcgcgag cgcagcgaag cgatccagtg ccacaaggat aaattctgga
    11401 ttgcttcacc acctttgg
//
LOCUS       DAOSWR010000063        11334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3637_length_11334_cov_7.431776, whole genome shotgun sequence.
ACCESSION   DAOSWR010000063 DAOSWR010000000
VERSION     DAOSWR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3637_length_11334_cov_7.431776"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..244
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..27
                     /rpt_unit_seq="gggccacgtcttttcagacgtggaaac"
     gene            complement(455..736)
                     /gene="cas2"
                     /locus_tag="PK513_05990"
     CDS             complement(455..736)
                     /gene="cas2"
                     /locus_tag="PK513_05990"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOO82032.1"
                     /translation="MYIVTYDICDDKRLRAVFRIMNGYGDHMQYSVFRCDLSDQEKIH
                     LVEKLHKILKHDEDQILFFPLGPSGGEREKSIYAIGRAYVPIERGPVIV"
     gene            complement(768..2444)
                     /gene="cas1"
                     /locus_tag="PK513_05995"
     CDS             complement(768..2444)
                     /gene="cas1"
                     /locus_tag="PK513_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOO82033.1"
                     /translation="MKTLENDIPDLVPARMVNEYVYCPRLAYIEWVQADFAENYDVAE
                     GRFRHRRVDEEKGALPAAIAKEDIIHARSVMLSSLGENLISRIDIIEGDATSVMPVDY
                     KRGAVPDIPAKAWDADRVQVCAQGLVLRDNGYTCNGGAIYYVESKTKVPVEFTDALIA
                     QTRQAVAGVRAMAKKGAIPPPLEDSPKCFRCSLAGICLPDETNMLAGRVTPDSDALRR
                     ILPARDDALPLYVQMQGARIGKSGEVFEVRAKNEKVGEARIFETSHVAIFGNVQVSTQ
                     AIQEMCSRGIPLTLFTTGGWYYGSATGMAHKNVELRIAQFAATTDQNKSLALAREMIS
                     AKIDNCRTLLMRNHQDLPDKVTSELKRYSESARKTDEASSLLGIEGMAARTYFSAFNG
                     MLKNNAGESWSFDFNGRNRRPPLDPVNALLSYAYALLVKDITVTLLAVGFDPYLGFYH
                     TLRYGRPSLALDLMEEFRPIVADSVVLWIINNRVLADEDFIKRGRMVALKAEARKKFI
                     SAYERRLDALITHPVFGYRISYRRVLEVQARLLGRFLTGEIGEYPSFRTR"
     gene            complement(2482..3540)
                     /locus_tag="PK513_06000"
     CDS             complement(2482..3540)
                     /locus_tag="PK513_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82034.1"
                     /translation="MTDFHIGQSLTHPDHGACTVTFVGTDYVGVEFNDGGNALIKKDA
                     FQYPPVPCEQTIVPTIRASTWPESTFVYEAPNAQHFLGSHWDAFFEDSKTILENLPTI
                     VQSAEPWIGGVNQEAPRQLPDNWPFGFVRAWPNHRQGLMMVIRISEQNELESFYPFAT
                     DGGQHSLKIRRIKVWESGVEAQIEVEVGKTTISFFDIAYAKHRLWYEAGKNYEFILMG
                     IAYGAKMSDVMEIPVSFHPDDIAWQKTLVDKDGEPLPEMSSMLSLRGVAMFMQVDGWD
                     ADDYSFRGSVKSVREVQGEILGQEGWFVRVTVLRFSDSDFDLDILITRRAWQGETPPV
                     VGQDIEGRLWLQGYLWDI"
     gene            complement(3537..5159)
                     /gene="csb2"
                     /locus_tag="PK513_06005"
     CDS             complement(3537..5159)
                     /gene="csb2"
                     /locus_tag="PK513_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOO82035.1"
                     /translation="MIGLAVSFPGGRYHATPWGRHVNEAAPEWPPSPWRILRTFVATW
                     KRKLDNDTGCAPQIVKDLMRKLAAPPLFVLPPASLGHTRHFMPWFKKGPTDRTLIFDG
                     FVALDKNHPVICLWPELELDQQESDVVDKIISNVVFLGRSESWTEARVLIHEEAAMAF
                     DNVNCMPVIDNYDKSKFDTVRVLCADPVTAFENSYTPKHTSIEGRGGTKQTIITPLYD
                     PDWHLCMETLELHDKRWSDPPGSCWATYLRLKDCFAVQPKRSRTVTARLRPTMARYAV
                     DGSVLPLVEDTLRVAESARRTAMGCFGRLGKKRLNNGNVPADAPLPRSEVFSGKDEQS
                     TPLEGHRHAYFLPTDEDGDGRIDHLTIIAAMGFGPAEVKALDRMNLLKRDDGDPLNLM
                     LIALGQSSEILAPIITGPSRVWISATPFIVTRFPKARGQKKDPVELLGLENQREFARQ
                     VLIEEIRRAYPDLIELVKVEYLNEEHRCGAHGLRPIQFKRYRQKRGDDGGSRPAGVFR
                     IVFPESVHGPICLGHSSHFGLGLFVPETDDNK"
     gene            complement(5156..6157)
                     /gene="cas7u"
                     /locus_tag="PK513_06010"
     CDS             complement(5156..6157)
                     /gene="cas7u"
                     /locus_tag="PK513_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HOO82036.1"
                     /translation="MSNGTIIDYKKMDAIPRLLMEAELKPLQGDRFQPTGFADLGAAR
                     YTLPNGTEMLLVESAQSVANRMELACWDEKNNDYISALKGLPFIRVNYGSQSITNSVL
                     EAHRINSEYIMNTSFKGLFSQEIKCQKDMPIDWPLLRKTLLKYDIGSLLHGSFLEEID
                     GRLRITRAISGFIEARDVSVAENGGVKNNFVQPTLKQGEGNVPFHRTEFTAKAITAYF
                     NLDLALLRGYGLDENATKLLIALSLFKVRRFLSTGLRLRTACDLDCIGLTVTRPEGFE
                     IPDEQVLLRECTRLIAACKSDGLFADPAVTVVTWEPSAKKGKAKENDEDNQDEEAAV"
     gene            complement(6182..8425)
                     /gene="csx17"
                     /locus_tag="PK513_06015"
     CDS             complement(6182..8425)
                     /gene="csx17"
                     /locus_tag="PK513_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HOO82037.1"
                     /translation="MNTIILKGCTPEPLMSYLKALGVLRIIAEGPDPDIRGCWRDGAL
                     TIKSILDEQSLIDFFDSAYQPTPVFAPWNGDGGFLTDSGASMELVNTIRETTYPNLMR
                     LKNAISSIDNVSILKDFKEQRDVKKILEDKKKKLKKTKQRLSESEAEQLKEATRLVNV
                     IKNDILYRIRTGFPDEVVRWLDSCVAITADGFNVSPLLGSGGVDGKLEMSANFMKNVV
                     AILDADQATRINWIKHAILGQGIVNLQKTAIGQFSPGHVGGPNATQGFEGDSGINPFD
                     FVLMIEGAIFISGAVSRRYGTDRSAKAAFPFTVYSSPVGYSSRADADAKGSRGEIWLP
                     LWNRLADIREVNYLFSEGRADLSGRQATSGVDFARAVSTLGVDRGIQGFTRYSFLQRN
                     GKAYLASPIGRFDVTARPNVDLLREIDPWLDRFRQKANEDKTPPRFKSALRRIETAIL
                     LFCQYGGPAHFADILCALGQAEKELANGENFRKDKYLRPLAGLSPAWLDAAYDTSPEF
                     EIALSLAGIYDASYKIGALRANLEPVKTGKNKQGSMGAAWLEGRCAVVWNSAELSRNL
                     AAVLERRIMDGERAGCDRLPLACRRTSSLAAIATFIAGATDDRRIEELLWGMLLINHY
                     KDYPILKRRAIDAPPLPRTYALLKLLFMPGPHTKQNESVEVRPEMTILPLLRAKRVDD
                     ACQIAIRRLRASGFIPQAHKTLHQWHTPDPARLAASLLIPVASRDVEYLKSMVLRPET
                     NNQAVTR"
     gene            complement(8422..10899)
                     /gene="cas3"
                     /locus_tag="PK513_06020"
     CDS             complement(8422..10899)
                     /gene="cas3"
                     /locus_tag="PK513_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOO82038.1"
                     /translation="MQTFKEMFKKATNKHQTPFHYQTMLAECRNFPSLLEVPTGMGKT
                     AAAILGWIWRRRYAPPAIRAATPRRLVYCLPMRVLVEQTRDCAVTWLYNLGILGGIAE
                     FEEKDGNIKLKDYDSWAGDDDTAKIRVHLLMGGEVDRDWDMYPERDAILIGTQDMLLS
                     RALNRGYALSRFRWPVQFGLLNNDCLWVMDEIQLMGNGLGTTTQLQAFRRMFTTAAPV
                     RSLWMSATMHEDWLKTVDFDVAQDAPGDAVSLEEDRKSDKFKKRFEAKKYITKAQFEI
                     DNDGKETARLASEVHVQGTLTLVVVNTVKRAQAIYKLLDKMKTAKKDGAMADLVLLHS
                     RFRQPDRNRQLNRLLAAAGEHGTIAVCTQVIEAGVDISARTLITDLAPWSSLVQRFGR
                     CNRYGEFNENGGGNIIWIPYANLADDKTLKPAPYLPEELRTAAKRLQDITEAGPYKLP
                     AFEDTLVFTHVPRRKDMIELFDTTPDLAGADIDVSRFIREADEYDVQVFWRDLPETGP
                     MSEEKGPAREELCTVPVSKELFQISQNKERHYWRWDHLERNWAKIKPRSVYPGLVLML
                     DSDQGGYSPELGWTGKEKVTESVDSILTDRPECNDEDKQSFSKVWQTIAEHTDIVNEK
                     LSTMLNEGLAELNEAERDALLEAARWHDAGKVHEQFQQAIPDEKRYQKTWAKSADVME
                     QYKRNGFRHELASALAMLANSKSDLAAYLAAAHHGRVRLSIRSLPQESRPPEMGRRFA
                     RGIWEGDVLPEAQLSAGLIMPPTELKLSYMELGEDPKTGPSWLSRMLKLRDDLGPFRL
                     AFLETILRIADWRASAHPMNDGKEETR"
     gene            complement(10995..11099)
                     /locus_tag="PK513_06025"
     CDS             complement(10995..11099)
                     /locus_tag="PK513_06025"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO82039.1"
                     /translation="MTDSTPSYYQYWGKVDKETGQYHLLPYHCLDVAA"
BASE COUNT         2739 a         2960 c         2634 g         3001 t
ORIGIN      
        1 gggccacgtc ttttcagacg tggaaacaat atcagaagcg ctgagaatgt gctcggctgt
       61 tttagcttca atggggccac gtcttttcag acgtggaaac gatccgcaag ccggggaagt
      121 attttgagtt taacacgctt caatggggcc acgtcttttc agacgtggaa acagaaagaa
      181 ggaaccgcat ttttgttgat ccaattgtgc ttcaatgggg ccacgtcttt tcagacgtgg
      241 aaactgcact acttcgaata atttgttttt aaagaacaat cagcattaat tgcgagatgt
      301 tccgtacaat tgttatcaat attatgtttt atcatctcaa gctacctaca agtgcaatat
      361 ttacttaatg cgagcagttc aagacctttt gacggcacgt cgccgctcgt aacgtcatgt
      421 cgtataaatc cgttattaac ttttcaaaga acatctacac gattacaggc cctctttcaa
      481 taggaacata tgcccggcca atcgcatata tacttttttc tctttcgccg cccgacggcc
      541 ccagagggaa aaataaaatt tgatcttcgt cgtgcttcaa aatcttatgc aacttttcca
      601 caagatggat tttctcctga tcggataaat cgcatctaaa aacagaatac tgcatatgat
      661 ccccgtaacc attcatgata cggaataccg cacgcaatcg tttgtcatca caaatatcat
      721 atgtaacaat atacattagc cgcataaatc caacacttca cggttaatta ccgcgttctg
      781 aaagatggat actcgcctat ttcaccggtc agaaaccgtc ccaggagtct ggcctggact
      841 tccagaacgc gacggtaact tattcgatag ccaaaaaccg gatgggtaat cagcgcgtcc
      901 agtcgccgct cataagccga tataaatttt tttcgggctt ccgcctttaa cgcaaccatt
      961 ctgccgcgct tgatgaaatc ttcatcggcc agaaccctgt tattgataat ccaaagaacg
     1021 accgaatccg caacaatcgg ccggaattct tccatcaaat ccagagccag cgacggtctt
     1081 ccgtatcgta atgtatgata gaatcccaaa tagggatcaa agcctaccgc caacaacgta
     1141 acagtaatat ccttgaccag cagcgcatag gcatatgaaa ggagtgcatt aacaggatca
     1201 aggggaggcc ttcggtttct tccgttaaaa tcaaaagacc atgactcacc ggcattgttt
     1261 ttcagcatcc cattgaaggc tgaaaaatac gttcgggccg ccattccctc aatacctaac
     1321 agtgaactgg cctcatctgt ttttctggcc gattccgaat accgtttcag ttcactcgtc
     1381 actttatcgg gtaaatcctg atgattgcgc atcaacagcg ttctgcaatt atcgattttt
     1441 gcagaaatca tctcacgcgc cagcgccaaa cttttatttt gatctgtggt ggctgcgaac
     1501 tgagcaatcc gaagttccac atttttatga gccatgccgg ttgccgaacc ataataccag
     1561 ccgccggttg taaacagcgt tagaggaata ccgcgactgc acatttcctg tatggcctgc
     1621 gtggatacct gcacattgcc gaatatcgca acatgcgacg tttcaaaaat gcgcgcctcg
     1681 ccgacttttt catttttggc tcgcacctca aagacttcgc cggactttcc gattctcgcc
     1741 ccctgcattt gcacatacag aggcagcgca tcgtcgcgtg cgggaaggat tcgacgcaag
     1801 gcatcggaat ccggcgtgac ccgcccggcc agcatgttgg tttcgtccgg cagacaaatg
     1861 ccggccagtg aacagcggaa acatttggga ctgtcttcca aaggcggcgg gatagcccct
     1921 tttttcgcca ttgcccggac accggcaacg gcttgacggg tttgtgcaat taacgcatcg
     1981 gtgaattcga cggggacttt tgtttttgat tcaacataat atatcgcccc gccgttgcag
     2041 gtatagccgt tgtcccgaag cacaagcccc tgtgcgcaca cttgcacgcg atcggcatcc
     2101 cacgcctttg cgggaatgtc cggtacggcc ccgcgtttgt aatccaccgg catgacagat
     2161 gttgcatccc cttctattat atctattctc gatatgagat tttcacccag cgaagacaac
     2221 atgaccgaac gcgcgtggat aatatcttct ttagcaattg ccgctggcaa cgcccctttt
     2281 tcttcatcca cccggcgatg tcgaaaacgc ccctcagcca catcataatt ttcagcaaag
     2341 tccgcctgca cccactcgat ataagccaga cgcgggcaat aaacgtattc gttcaccatt
     2401 cgggcgggaa cgaggtcggg gatatcattt tccagagttt tcatgcgttt ctattccaga
     2461 ttcagtttga aagcattcac attatatatc ccagaggtag ccttgcagcc atagacgacc
     2521 ttcaatatct tggccgacga caggcggggt ttcaccctgc caagcacgtc ttgtgattag
     2581 aatatccaaa tcaaaatcac tatcactaaa acgcaaaacg gttactcgaa caaaccagcc
     2641 ttcctgaccg agaatctcgc cctgaacttc tctgacagat ttaacggatc ccctgaaact
     2701 gtaatcgtcg gcatcccatc catctacctg cataaacatg gccacgccgc ggaggcttaa
     2761 catcgatgac atttccggca acggttcgcc atccttatcc acaagggttt tttgccatgc
     2821 gatatcgtcg gggtggaagc ttactggtat ttccatcaca tcagacatct ttgcgccata
     2881 cgcgataccc atcagaataa attcataatt ttttccagct tcgtaccaca gcctgtgttt
     2941 tgcataggca atatcgaaga aggagatggt cgttttccca acttcaacct caatttgcgc
     3001 ttcaacgccg ctttcccata ctttgatccg cctgatctta agcgaatgtt ggccgccgtc
     3061 ggtcgcaaag ggataaaaac tttcgagttc attttgttca ctaattctga tgaccatcat
     3121 taaaccctga cgatgattag gccaagccct cacaaaaccg aatggccaat tgtcaggcaa
     3181 ttgtcgaggc gcttcttgat taactccgcc tatccacggt tcagcgcttt gcacgatggt
     3241 tggtaggttt tcaaggattg tctttgaatc ctcaaaaaac gcatcccagt gtgagccgag
     3301 aaaatgctga gcattgggag cctcatacac aaaagtggat tcaggccatg tcgatgctcg
     3361 gatagttggg actatagttt gttcacatgg gacgggtggg tactggaagg cgtctttctt
     3421 aataagggca tttccaccat cattgaattc aacccccaca taatctgtgc ccacaaatgt
     3481 aaccgtgcat gcgccgtgat caggatgcgt aagactttgg ccgatatgaa aatctgtcat
     3541 ttattgtcat ccgtttcagg tacgaataaa cccagaccaa aatggctgga atgaccaaga
     3601 cagatcgggc catgcactga ctcgggaaag acaatacgaa aaacgccggc tggacggctg
     3661 ccaccatcgt ctccgcgctt ctgacgataa cgtttgaatt ggatcggccg cagaccatgc
     3721 gcaccgcaac gatgttcttc attaagatat tcaaccttta ccagttcaat cagatccgga
     3781 taagcacgtc gtatttcctc aatgagaacc tgacgggcga attcgcgctg attttcaagg
     3841 ccaagcaatt ccacggggtc ttttttctgt ccgcgtgctt taggaaaccg cgtgacgatg
     3901 aaaggcgtgg ctgaaatcca gacacgagaa ggccctgtaa ttattggcgc aagaatttcg
     3961 gaagattgac caagcgcaat gagcataaga ttcaaaggat cgccatcatc gcgcttgaga
     4021 aggttcattc ggtccagcgc ttttacctcg gccggtccga accccatggc cgctatgatg
     4081 gtaagatggt caatacgccc atcgccatct tcatcagtgg gaagaaagta ggcatggcga
     4141 tgcccttcaa gaggtgtgct ctgttcgtct ttacctgaga aaacctcaga gcgggggagc
     4201 ggtgcatcag ccggtacatt gccgttattg aggcgcttct ttccaagacg tccaaagcac
     4261 cccatggcgg ttcggcgagc tgattccgca acccgtagcg tatcttccac cagtggcaga
     4321 acactcccat cgacggcata gcgcgccatt gttggacgta accgtgcagt aacggttctt
     4381 gatcgttttg gttgcactgc gaaacagtcc ttaagtctca ggtatgttgc ccagcaagag
     4441 cccggcgggt ccgaccagcg cttgtcgtgc agttccaggg tttccatgca cagatgccaa
     4501 tccggatcat aaagtggcgt aataatcgtc tgttttgttc ccccccggcc ttcaatactc
     4561 gtgtgttttg gggtgtatga attttcaaaa gcagtcaccg gatcagcgca gagtacacgc
     4621 accgtatcga attttgactt gtcgtaatta tctatcacag gcatacaatt tacattatcg
     4681 aaagccattg cagcctcctc gtgtatgagc acacgcgcct ctgtccatga ttcggaacga
     4741 ccaagaaaaa ccacatttga aattatctta tcgacaacat cgctttcttg ttggtcaagt
     4801 tccagttccg gccagagaca gataacggga tgatttttat ccagcgcgac aaagccgtca
     4861 aagatcaatg tgcgatccgt cggtcctttt ttaaaccatg gcatgaaatg ccgcgtgtgg
     4921 ccgagggatg cggggggcag tacaaaaagc ggaggtgcgg caagtttacg catgagatcc
     4981 ttgactattt gaggcgcaca tccggtatca ttatcgagct tgcgcttcca tgtcgccacg
     5041 aaagtacgca aaatacgcca gggtgacggc ggccattcag gcgcagcctc attaacgtgc
     5101 cgcccccagg gtgtggcgtg ataacggcct cctggaaagc tgacagctaa gcctatcata
     5161 ccgctgcctc ctcatcttga ttgtcttcat cgttttcttt ggccttgccc ttctttgcac
     5221 tgggttccca agttacaacc gttacggccg gatcagcaaa gagcccatcg cttttacaag
     5281 ccgctatcaa gcgggtacac tccctaagca gcacctgttc atcgggtatt tcaaaacctt
     5341 cgggcctggt tactgtcaat cctatgcaat caagatcgca ggctgtgcgc aagcgaagtc
     5401 cggtggaaag gaaacggcgt actttaaaaa gcgacagggc aatgaggagt tttgttgcat
     5461 tctcatcaag cccgtaacca cgcaacaatg ccagatcaag gttgaaatat gcggtgatcg
     5521 ctttagccgt gaactcggtg cggtggaagg gaacattgcc ctcaccctgc tttagggtcg
     5581 gttgtacgaa gttatttttc acgccgccat tttccgcaac cgacacatct cttgcttcaa
     5641 taaagccact tattgcacga gtaatgcgca aacgaccgtc aatttcctct aagaaagacc
     5701 cgtgcaacaa agagccgata tcgtacttca agagcgtctt tcgcaatagc ggccagtcaa
     5761 taggcatgtc tttttgacat ttaatttctt gagaaaaaag ccctttgaat gatgtgttca
     5821 taatgtattc agagttgata cgatgagcct caagcactga gtttgtgatt gattgagatc
     5881 cgtaatttac gcgaataaac ggtaatcctt ttagcgcact gatataatca ttgttctttt
     5941 catcccagca agccaactcc atgcggtttg cgacggactg tgccgactct accagaagca
     6001 tttccgtgcc attcggcaaa gtatagcgcg cagcgccaag atcggcgaat cccgtgggtt
     6061 gaaagcggtc cccctgcaaa ggtttaagtt ctgcttccat tagaagcctc ggaattgcgt
     6121 ccattttttt gtaatcgatg attgttccgt ttgacatgaa tatttctcct ttcgtgttcc
     6181 gtcatcgggt gacggcttga ttatttgttt ccggtctcag gaccatactt ttcagatatt
     6241 ctacgtcgcg cgaagcgact ggtataagca aagaggcggc aaggcgcgcg ggatcgggtg
     6301 tatgccattg atgtaacgtt ttgtgtgctt gcgggataaa accactggcg cgcaaacggc
     6361 gtattgcgat ttgacaggca tcatccacgc gtttggcgcg aagcaacggc aggatagtca
     6421 tttccggccg gacctccacg ctttcgtttt gtttggtgtg tggcccgggc ataaacaaca
     6481 gtttaagcag ggcgtaagta cgcggcagcg ggggggcatc aatggcgcgt ctctttaaga
     6541 tggggtaatc cttgtaatga ttgatcagta acatgcccca gagcaattcc tcaatacgtc
     6601 tgtcatctgt cgctcctgca ataaaggtgg caatggcagc gagtgaggac gttcttcggc
     6661 aggcaagggg taatctatca catccggcgc gctcgccgtc catgatccgc cgttcaagca
     6721 cggcagccag gttacggctc aactctgctg agttccaaac caccgcgcat ctgccttcca
     6781 accatgcggc acccatactt ccctgcttat tttttcctgt ctttaccggt tccaagttgg
     6841 cgcgcagcgc accgatcttg tatgaagcat cgtaaatgcc ggccaaagac agggcaatct
     6901 caaattccgg tgaagtgtca taagccgcgt caagccacgc tggcgataat ccggccaaag
     6961 gccgcaaata tttatccttc cgaaaatttt cgccattggc gagttctttc tctgcttgcc
     7021 ctaaggcgca caggatgtct gcaaagtgcg caggcccgcc atattggcag aaaagaagga
     7081 tggcggtctc aatccttcgc agtgccgatt tgaaacgcgg cggggtttta tcttcgttgg
     7141 ctttctggcg gaagcggtcc agccacgggt cgatttcgcg aagaaggtca acatttggcc
     7201 gagccgttac atcaaaacgc ccgatgggcg acgcaaggta ggcttttccg ttccgctgca
     7261 gaaaactata tctggtaaat ccttgaattc ccctatccac acctagggta gagacagccc
     7321 ttgcgaaatc aaccccagat gtggcctgcc tgcctgaaag atcagcccga ccttctgaaa
     7381 ataaatagtt aacttctctg atatctgcca gacgattcca taagggcagc catatctcac
     7441 cccgtgagcc cttggcatcg gcatccgctc ttgaactgta tccgactggt gaactataca
     7501 ccgtgaaggg aaatgccgct ttggcggacc tgtctgttcc atagcggcgt gaaactgcgc
     7561 cgctgataaa tattgcgccc tcgatcatca gcacgaagtc aaaggggttg attcccgaat
     7621 ctccttcaaa tccttgggtt gcattcggtc cgcctacgtg cccaggtgag aactggccaa
     7681 tcgctgtttt ctgaaggttt acgattccct gccccaagat agcatgctta atccagttta
     7741 ttcgggtcgc ttgatcggca tctaatatcg ctacgacatt tttcatgaaa ttcgcggaca
     7801 tttctagctt cccgtcaaca ccacctgaac cgaggagcgg tgaaacgtta aagccatcgg
     7861 ccgtgatcgc gacacatgaa tccaaccagc ggacaacctc atctggaaaa ccggttctaa
     7921 tgcgatacag aatgtcgttt tttatcacgt tgactaaacg agtggcttct ttcaattgct
     7981 ctgcttcgct ctcactgagt cgttgtttcg tcttttttag ttttttcttc ttatcctcaa
     8041 gtattttctt aacatcacgt tgctctttga aatcttttag aatggaaacg ttatcaatgc
     8101 tgctgatcgc attcttcagc cgcatcagat taggatatgt tgtttcgcgg attgtattaa
     8161 cgagttccat actagccccg ctatccgtca ggaatccgcc gtcaccattc catggcgcaa
     8221 ataccggggt tggttggtaa gcactatcaa agaaatcaat caacgattgt tcatccaata
     8281 tcgatttgat ggtgagcgcc ccgtcccgcc aacaaccgcg aatgtccggg tctggtcctt
     8341 cagctataat cctcaggaca cccaatgctt tgaggtaact catgagcggt tccggtgtgc
     8401 agcctttcag gataatcgtg ttcatcgggt ttcctccttt ccgtcattca ttggatgggc
     8461 gcttgcacgc caatcggcaa tgcgcaggat ggtttccaga aatgccagcc ggaatggtcc
     8521 cagatcgtcg cgcagcttga gcatgcgcga aagccagctt ggtccggttt tcgggtcttc
     8581 tccgagctcc atatagctga gcttcaattc agttggcggc atgataagtc ccgcggagag
     8641 ttgcgcttcc ggaagcacat cgccctccca gatgccacgc gcaaagcgac ggcccatttc
     8701 cggtgggcgc gattcctgcg gcaaagaacg aattgaaagt ctcacccggc cgtggtgtgc
     8761 ggcggccaga taagcggcaa ggtctgattt ggaattggct agcatcgcca gcgccgaggc
     8821 cagttcgtga cggaaaccat tgcgcttgta ttgctccatc acatctgcag attttgccca
     8881 cgtcttttga tatcgctttt cgtccggaat cgcttgctgg aactgctcat gtacctttcc
     8941 tgcatcatgc cagcgcgctg cttccagaag tgcatcgcgt tcagcttcat ttagttccgc
     9001 caagccttca tttaacattg tcgagagctt ttcattaaca atgtccgtat gttctgcaat
     9061 ggtttgccat acttttgaaa aagattgttt atcttcatca ttacattcag gcctgtcagt
     9121 taggatgcta tcaacggact ctgtgacttt ctcttttccc gtccagccca attccggact
     9181 atagccgcct tgatcggaat ccagcattaa caccaaaccg ggataaactg atcgaggctt
     9241 tatctttgcc cagtttcttt ccaggtggtc ccaacgccaa taatgccgct ccttgttctg
     9301 tgatatctgg aaaagttcct tgctgaccgg tacggtgcaa agttcttcgc gggctgggcc
     9361 tttctcttct gacataggac cggtttcagg cagatcacgc cagaacacct gcacatcata
     9421 ttcatccgcc tcgcggataa agcgagagac atcgatgtcc gcaccggcca agtccggcgt
     9481 ggtgtcgaag agttcgatca tgtctttgcg gcgcggtaca tgcgtaaata ccagtgtgtc
     9541 ttcgaaagcg ggcagtttat aagggccggc ctcagtaatg tcttgaaggc gcttagccgc
     9601 agtgcggagc tcctcaggca gatacggcgc cggttttaat gttttatcat ccgcgagatt
     9661 tgcataggga atccaaatga tatttcctcc tccattttca ttaaattccc cataccggtt
     9721 gcagcgtccg aaacgctgca caagcgatga ccagggcgca agatcggtaa tcagcgtccg
     9781 ggccgagata tctacgcctg cctcgataac ctgagtacag accgcaatgg ttccgtgttc
     9841 accggctgcg gcaagcaaac ggttcaattg cctgttgcgg tcaggctgcc ggaagcggga
     9901 atgcagcagg acaagatcgg ccatcgctcc gtctttttta gcagtcttca ttttatcgag
     9961 tagcttgtaa attgcctgcg cgcgtttcac ggtattgaca acaaccagag tgagggtgcc
    10021 ttgaacatgg acttcactgg ctaaccgcgc ggtttccttt ccgtcattgt cgatttcaaa
    10081 ttgtgccttt gtgatgtatt ttttcgcctc gaatcttttt ttgaacttat cgctttttcg
    10141 gtcttcttcc agcgatacag cgtcaccggg cgcatcctga gcaacgtcaa aatccacggt
    10201 cttgagccaa tcttcatgca tggtggcgct catccagagc gagcgtaccg gcgcggcggt
    10261 tgtgaacata cgacgaaagg cctggagttg cgtggtggtg cccaaaccat tgcccatcag
    10321 ttggatttcg tccataaccc aaaggcagtc gttattgagc agaccgaact ggacaggcca
    10381 gcggaaccgg cttagcgcat agccgcggtt gagcgcgcgg gaaaggagca tgtcctgtgt
    10441 gccgatcagg atggcgtccc gctccgggta catgtcccaa tcacggtcaa cctcgccgcc
    10501 catgagcaaa tgcacgcgaa tcttggccgt gtcatcgtct ccggcccagg aatcatagtc
    10561 tttcaatttg atattaccgt ctttttcctc aaattccgca atgccaccta atattcccaa
    10621 gttatacagc catgttacgg cgcaatcccg tgtttgttcg accaaaacac gcatcggcag
    10681 gcaataaaca aggcgtcgtg gtgttgcagc tcggatagcc ggtggagcat atcgcctgcg
    10741 ccatatccag ccaagtatgg cagccgctgt tttccccata ccggttggaa cttcgagcaa
    10801 tgatggaaag ttacgacact cggccagcat ggtctgataa tgaaatgggg tctgatgttt
    10861 gttggttgct tttttaaaca tttctttaaa agtttgcatc tatgcacctc atggttttta
    10921 cgttaagctt tattggattt cggtttcgct tccgcaccga ctatctttcc ttcgttagtc
    10981 ttttttgtat tttatcacgc cgccacatcc aaacaatggt aaggcaggag atgatattgt
    11041 ccagtttcct tatctacttt tccccagtat tgatagtatg aaggtgttga gtcagtcatt
    11101 tcttttccga aatatttcaa attagcagat ttcttatttt ttccattgta atgttcttat
    11161 tttaccaccc caccaccccc aatttatgtc ccatcaaaac gttgctcaaa agatatttta
    11221 tggatttaaa atgataaaac gttcctgaaa tttccgagac agcgtttatt catttcaaaa
    11281 ctgatctggt caagtaaaaa cggacacccg gttaagctgc ttttgccaac gtaa
//
LOCUS       DAOSWR010000064        11086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3734_length_11086_cov_5.080319, whole genome shotgun sequence.
ACCESSION   DAOSWR010000064 DAOSWR010000000
VERSION     DAOSWR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_11086_cov_5.080319"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1516)
                     /gene="der"
                     /locus_tag="PK513_06030"
     CDS             complement(77..1516)
                     /gene="der"
                     /locus_tag="PK513_06030"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOO82040.1"
                     /translation="MFTLALVGRPNVGKSALFNRLAGKKLAIVDDTPGITRDWRSAEG
                     ALFDHTLRIIDTAGLEESFDDSIQGRMRQQTEAALKNADAVLFIIDGRAGLTPLDEHF
                     AGWLRKQKKPVILAVNKGENEKAIATTIGEAFRLGLGEPIGISAEHGIGMEELYHALE
                     PLISKTDTTNDGEENSLSFEGEGWGEEGLDDLEGCEDFTFNQDEDDPEKPLKIAIVGR
                     PNVGKSTLLNSIVNDQRVMTGPEAGITRDAIAVDWDYKDRSFKLVDTAGMRRKSKVQD
                     NIEKMSVEDSMRAIRLAQIVILVIDGNAIFEKQDLQIAEHIINEGRALVVAVNKWDSV
                     RDRDEALEELAYKAETSLAQLKDIPYQTISALNGKNINKLLDRCLDTYTIWNKRISTA
                     GLNRWLAKMESQNPAPLISGRSNRLKYITQIKTRPPTFALWVARPDKLPESHKRFIIN
                     GLRRDYKIPGVPIRLLVRKSKNPYAETKK"
     gene            complement(1550..2875)
                     /locus_tag="PK513_06035"
     CDS             complement(1550..2875)
                     /locus_tag="PK513_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO82041.1"
                     /translation="MIKRCLCLTSLVLLTVFLSGCGASGWFEKDGPPPLEGERISVMQ
                     MQKNLEPDNPVLESQGLLAPAEWNNEFWPQVGGYPNHSMQNLSLPASPLKRLWSANIG
                     DGATDELPLITQPIIIDNRIFTLDTDNRLSAFNIENGKKLWDTDVQAENEDDPVIAGG
                     IAFSGSMLYVTNGYNEVLAVKPDDGQIIWRKTIPAPSRAAPTIMEERVFVTTLDGRVM
                     ALNAKDGSILWEYTGISDDATLVGAASPAANQDIVVPVFSSGEITALRIENGSIAWSD
                     NLSNVRSFGGLSTISDIRALPVMDKGVVFAISFSGRLVAIDERTGTRIWQREISGSQT
                     PWMAGNHIFVLSSENQLIALGRENGSIRWTTELPRFEDDDPQVFNGPVLAGGRLILAG
                     TDGRVIEIAPENGKILGEWDAGATVSTSPVIAGGTLYLLTNDGTLTAYR"
     gene            complement(2882..3562)
                     /locus_tag="PK513_06040"
     CDS             complement(2882..3562)
                     /locus_tag="PK513_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82042.1"
                     /translation="MADLFREVDEAMRQERVEKFWAENKTYIIGVILGTILLTGLISG
                     YRSWNSSVKEKQTAALIALQESPEYPDNILTAEKLPMRAGLRGIAFLQAAGKFLDQDK
                     TEDALMLYKRISHDKALPDELKDLGTLMSVRLQIDQDDQGGQALLDQLIPLISNPQSP
                     WVHHARIEAALITADKLGDYKGALTHLNAVLDTPDLPQSLTGRARALAHLYDMKNKSV
                     SEKQDETG"
     gene            complement(3640..4191)
                     /locus_tag="PK513_06045"
     CDS             complement(3640..4191)
                     /locus_tag="PK513_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO82043.1"
                     /translation="MALLETPLKDDNFYAPDFKLQNIDGKFLTYNDIKGKKGTVIAFI
                     CNHCPYVQAIIERFVQDAQTLQDLGIAVAAIMPNDTGNYPADSQENMVLFAEENGFTF
                     PYLIDETQEVARSYGAVCTPDIFGFTANGQLQYRGRLDSAGPNEATSKTVKELVNAMT
                     QIAQDGVGPANQTPSMGCSIKWK"
     gene            4232..4564
                     /locus_tag="PK513_06050"
     CDS             4232..4564
                     /locus_tag="PK513_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82044.1"
                     /translation="MSEIIPFSRASHASGFTNAQFKKLDGLYLRAAAAGVLTYRSVEC
                     DFDEGVASYTYCKSEHMSPYLQFVIRKVGPRATMYEVYLQDKGRVIKSGVFDKAFARL
                     QDEVEKLF"
     gene            4833..5582
                     /locus_tag="PK513_06055"
     CDS             4833..5582
                     /locus_tag="PK513_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82045.1"
                     /translation="MNAGGGADFFDYWFVFETFMTSVFMVALYRFFILDEKPDIKYIE
                     YKKGIPELKTVFKLPYYFRFGRREFVFALFSSLLGVFFLLLQDVFRLYWLNNLAADGL
                     SGWIQADLTHSAFLYTGIVYFLQFLELLVYTVLVLVFPFLALHDSYAFKNISEMVKAV
                     KGNIFRILIIVSLIYLPYDGFNSAINLVYVYAFKMGLLSDSSPFREDILFHVGWIRYY
                     ASLGVYYVCVLADIIFLSIIYKRLVLENDKN"
     gene            5569..6780
                     /locus_tag="PK513_06060"
     CDS             5569..6780
                     /locus_tag="PK513_06060"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOO82046.1"
                     /translation="MTKIKVKNPIVEIDGDEMTRIIWAFIKERLILPYLDVDLKYFDL
                     SIQSRDASDDQITVMAANAIKEHGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNI
                     LNGTVFREPITTANVPKYVPGWIEPIVIGRHAFGDQYKATDIKVPGPGKLELVYTPEG
                     GEPEVHEVFDFPSSGVGMAMYNLDESIEGFARSSFNYGLSRKYPVYMSTKNTILKQYD
                     GKFIEIFQQIFDEEFKADFEKHDLWYEHRLIDDMVAQAIKSKGGFVWACKNYDGDVQS
                     DIVAQGFGSLGLMTSVLLTPDGKCVEAEAAHGTVTRHYREHQKGNPTSTNPIASIFAW
                     TRGLKYRGEFDGTPEVVDFADTLERVCVETVEAGHMTKDLALLIGEQQKWLTTQEYLD
                     KVVAGLEKAMQ"
     gene            6781..7938
                     /locus_tag="PK513_06065"
     CDS             6781..7938
                     /locus_tag="PK513_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOO82047.1"
                     /translation="MRTVYVFQILVSVFLALVSAQAYAGYDKPYVGDMIEYDAKYEDT
                     FIHLARDYNLGFTELRAANPYVDPWMPGRGTDLILPTRHLLPDASREGIVINLADMRL
                     YAFINGDEAPYHTPIGVGREGLSTPEGVTEIVRKVEGPVWRPTPRMRKADPTLKEAYY
                     QGPDNPMGTHALYLGWPQYALHGTNRPFGIGRRVSSGCIRLYPEKISELFELIPVGTK
                     VTVVNQPIKVAWIDDELFLEANPSLAQSVAMEEYGEVPEEKISNDEMRLIVKTAGDWQ
                     DRLRWPAIRKALKERNSYPVSIARRPSLEVKGDVIIEHEKAAFIETPEEAKDLIEGYE
                     PAPEKRDDIEKAQDKNSKTGEDTDKIRMAEGETTEQDQDQDQDNPARMLNP"
     gene            complement(8331..8528)
                     /locus_tag="PK513_06070"
     CDS             complement(8331..8528)
                     /locus_tag="PK513_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82048.1"
                     /translation="MKKFAATLCIAGTAIALSACDTTKMGDVDTAPPYASERTAGYAS
                     EPAPAPAPAPAERVFREYQTK"
     gene            complement(8744..9934)
                     /locus_tag="PK513_06075"
     CDS             complement(8744..9934)
                     /locus_tag="PK513_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO82049.1"
                     /translation="MILLLTLLSPLNARALNIESVRFGLYEEKTRLVLDLDKPADFRA
                     FVLSEPYRMVIDLPSFEWQAGKVQSPQDAGVQATRHGSLKPGISRIVFDLNRPISIQK
                     AFTLPGGAGKPDRLVIDFSNVSPSLFEQIKSTVHGSLDPDRIQTQNANIATLAPPPPP
                     KPQDQKPLIIIDPGHGGIDPGASGPHGLYEKNVVLALAKDLKVQLESSGKYRVKMTRA
                     DNHFIKLSNRVKFARKHGGDLFISLHADSMENKSITGASVYTLSEKASDAQTAKLAAR
                     ENKADLIAGIDLSIEDEDVVGILMDLAMRDTMNQSKFFANTLVNSFNANGVKTLPRPH
                     RYAGFAVLKAPDIPSVLIEAGFMSNEKEATLLNTSHHRQKIATALTKGIDRYFEQVRK
                     NQRI"
     gene            10343..>11086
                     /locus_tag="PK513_06080"
     CDS             10343..>11086
                     /locus_tag="PK513_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO82050.1"
                     /translation="MSKKMLIDGTHSEETRVAIVEDGRLEEFDYESAFRKPLKGNIYL
                     AKVTRVEPSLQAAFVNFGGNRHGFLPFSEIHPDYFRIPVSDREALIAEQEADLEAHDE
                     EEALEEQELPEDDVDEEDAVEELGGDLTLEDGDKDGESEEEDVKAEKKPRGGRKSKKD
                     TEVDTADAVDAVEEDPEDSIGNRIEEPEADIDGNNADAQGEDADGNMYGGRGRGRGRG
                     RRDNGRGRGGRSRNAAHRARRVETFGGEDM"
BASE COUNT         2682 a         2256 c         3059 g         3089 t
ORIGIN      
        1 ccccggcttt tcgccggggt ttcgttcttc tttctatttc aatcctttaa aagaagttga
       61 gaattaggca caatcactat ttttttgttt ctgcgtacgg gtttttagat ttgcgtacaa
      121 gcaggcgtat ggggacgccg ggaatcttgt aatcacgacg cagaccattg ataatgaatc
      181 gtttatgcga ttcaggcaac ttatcaggtc gggcaaccca cagggcgaaa gtcggcggac
      241 gggttttaat ctgggtgata tatttcaaac ggtttgaacg tccggagatc agcggagcgg
      301 gattttggct ttccattttt gccagccagc ggttgagacc ggcggtagag atacgcttgt
      361 tccaaatagt gtacgtgtcc agacagcgat caagcagttt gttgatattc ttgccgttaa
      421 gggctgaaat agtttggtag gggatgtctt tgagctgggc gagtgaagtt tcagccttgt
      481 atgccaattc ttcaagggct tcatcacgat cacgcacgct atcccatttg ttgacggcaa
      541 caaccagcgc gcggccctca ttgatgatat gttcggcaat ttggagatct tgtttttcaa
      601 aaatcgcatt gccgtcgatg acgaggatga cgatttgagc gaggcgaata gcgcgcatgg
      661 aatcttcgac gctcattttc tctatattat cctgaacctt gcttttcctg cgcataccgg
      721 cggtgtcaac caatttgaag ctgcggtctt tataatccca atccacagca atagcatcgc
      781 gggtaatacc ggcttcgggg ccggtcatga cgcgctggtc attgacgatg gaattgagca
      841 acgtggattt tccgacattg gggcggccga caatggcgat tttaagcggt ttttcaggat
      901 cgtcttcatc ctgattgaaa gtaaaatctt cacagccttc gagatcatct aatccctcct
      961 caccccaacc ctctccctca aaggagaggg aattttcttc accgtcgttg gtggtgtcgg
     1021 ttttggaaat gagaggttcg agcgcatggt aaagttcttc cataccgatg ccgtgttcgg
     1081 cggagatacc gatgggttcg ccaagaccta gccgaaaggc ttcaccgatt gtggttgcaa
     1141 tcgctttttc gttttcacct ttattaacgg ctagaatcac aggttttttt tgtttgcgca
     1201 accaacctgc gaaatgttcg tcaagcgggg tgagaccggc gcgaccatcg atgatgaaga
     1261 gtacagcatc agcgtttttg agagcggcct cggtttgctg gcgcatacgg ccttgaatag
     1321 agtcatcaaa gctttcttcg agaccggcgg tgtcgatgat gcgtaaggtg tggtcgaaca
     1381 gcgccccttc agcgctgcgc caatcgcgag tgatgcccgg cgtatcatcg acaatcgcca
     1441 gcttttttcc ggccaggcga ttgaagaggg ctgactttcc tacgtttggt cttccaacga
     1501 gcgcgagtgt aaacatgtgc gcttgtttac atggttcttg tgaaactgtc tagcgataag
     1561 cggtcagggt tccgtcattg gtgagcaggt agagcgttcc accagcaata acagggcttg
     1621 tcgatacggt ggcgcccgca tcccattcgc ccaggatttt gccgttttcc ggcgcgattt
     1681 cgatgacgcg gccatctgtt ccggctaaga ttaaacgtcc tccggccaga acggggccgt
     1741 taaaaacttg aggatcatca tcttcgaagc gtgggagttc tgtcgtccag cggatggagc
     1801 cgttttcgcg gcccagcgca atgagctggt tttcggagga tagcacaaag atatgatttc
     1861 cggccatcca cggggtttgc gatccgctga tctcgcgttg ccagatgcgt gtgccggtgc
     1921 gctcgtcgat agcgacgagg cggccggaaa agctgatggc aaaaacgaca cctttatcca
     1981 ttacaggcaa agcgcggatg tctgaaatgg tcgacaatcc gccaaagctg cggacatttg
     2041 agagattgtc cgaccaggca atggaaccgt tttctatacg caaggcggtg atttcaccgg
     2101 atgaaaagac gggcacgacg atgtcctgat tggccgctgg agaggctgcg ccgactaagg
     2161 tggcgtcatc gctgatgcca gtatattccc acaggatgct gccgtctttg gcgtttaaag
     2221 ccatgacacg gccatcaagt gtggtgacaa agacgcgttc ttccatgatg gtgggagcgg
     2281 cgcgtgacgg ggcgggtatg gttttgcgcc atatgatctg gccatcatcc ggttttacag
     2341 ctaacacttc gttatagcca ttggtgacgt acagcatact gccactgaaa gcaataccgc
     2401 cggcaataac agggtcatct tcgttttcgg cctgcacgtc tgtgtcccag agttttttgc
     2461 cgttttcgat attgaaagca gacaggcggt tgtcggtatc gagtgtaaat atccgattat
     2521 cgatgataat gggttgtgta ataagtggca gttcatcagt ggcaccatcg ccaatattag
     2581 cactccaaag acgttttaaa ggactggcag gcaaggagag gttttgcatt gaatggttcg
     2641 ggtaacctcc gacttgcggc cagaattcgt tgttccattc tgctggcgct aaaagcccct
     2701 ggctttccag aaccggattg tccggttcga gatttttctg catttgcatg acgctgatgc
     2761 gttcaccttc aagcggtggt gggccgtctt tttcaaacca gccggaagcg ccgcagccgg
     2821 agaggaaaac ggtcagaagg acaagggaag taaggcatag acagcgtttg atcatgggat
     2881 attatcctgt ttcatcttgt ttttctgata cggatttatt tttcatgtcg tacagatgcg
     2941 ccagggcacg agcacgacca gtcaggcttt gtggcaaatc aggcgtatcg agcacagcat
     3001 tgagatgtgt aagtgcgccc ttgtaatcgc caagtttatc ggcggtgata agagccgctt
     3061 cgatacgggc atgatggacc catgggcttt gaggattgct tatcagcggt atgagttgat
     3121 caagcaaggc ctggccaccc tgatcgtctt ggtctatttg caagcgtacg ctcatgagtg
     3181 tgccgagatc tttaagttca tcgggaagtg ctttgtcatg gctgatgcgt ttatatagca
     3241 tgagagcgtc ttcggtttta tcctgatcga ggaattttcc ggctgcttgc aaaaaggcta
     3301 taccgcgcag gcctgcgcgc attggaagct tttcggcggt caaaatgttg tcgggatatt
     3361 ccggggattc ttgcagggca atgagcgcgg cggtttgctt ttctttgaca ctggaattcc
     3421 aactgcgata gccggaaatt agcccggtca gtaaaattgt gccgagaatg acgccgatga
     3481 tgtaggtttt attttcggcc cagaatttct cgacgcgctc ctggcgcatt gcttcgtcta
     3541 cctcgcggaa caagtcggcc atgtgttact ccttctattt cttcgcttct ttttagcatg
     3601 aagatcaaaa aagtcacgcg aagatcgcaa cgttattttt tatttccatt taattgaaca
     3661 acccatagat ggggtttggt tggcggggcc gacgccatct tgagcgattt gtgtcatggc
     3721 gtttacgagt tctttgaccg ttttggaggt cgcttcgttg gggccggcgg aatcaagacg
     3781 gccgcgatat tgaagctggc cattggcagt aaagccgaag atgtcggggg tgcatacggc
     3841 gccatagctt ctcgcgactt cttgcgtttc gtcgattaaa taagggaagg tgaagccgtt
     3901 ttcttcagcg aagagcacca tgttttcttg cgagtcggcc gggtagttgc cggtatcgtt
     3961 gggcatgatg gcggcgacgg caatgccgag atcttgcaga gtttgggcgt cttgtacaaa
     4021 tcgttcgatg attgcctgaa cgtacgggca atggttgcag atgaaggcga taacggtgcc
     4081 ttttttgcct ttaatgtcat tataggtcag aaatttgccg tcgatatttt ggagtttgaa
     4141 atccggagcg tagaaattgt cgtcttttaa aggtgtttcg agtaaggcca tggctgttct
     4201 cctttactaa aaaaagtggt atgatgaaca tatgtcagaa attatccctt tttcaagggc
     4261 gagtcatgct tcaggtttta ccaatgcgca gttcaagaaa cttgacggat tgtatttgcg
     4321 cgcggcagca gcgggcgttt tgacttatcg cagtgtggag tgcgattttg atgagggcgt
     4381 ggccagctat acctattgca aatccgagca tatgagcccg tacctacagt ttgtgatccg
     4441 taaagtgggg ccgcgcgcaa cgatgtatga ggtatatttg caggataagg ggcgcgtgat
     4501 taaaagcgga gtgtttgata aagcgtttgc acgccttcag gatgaagtgg aaaaattatt
     4561 ttaaccacct ctgaacacag catagacaca gatgttttga gcatatagcg tatctgtgtt
     4621 aatccgtgtg tatctgtggt tttttaaaag gtttttttat gtccaagatc aaagttaaaa
     4681 tctggccaat ggtcaaggaa tcttatgcgc aagtgaatag aacacgcgct atctggtcgc
     4741 ctgttgtctg gttgcctctg gctttgattt ttgtgtggta ttctatattc tggttttttc
     4801 ccgaatttcg tctttttatc agcacggacc tgttgaatgc aggcgggggc gccgatttct
     4861 ttgattactg gtttgtcttt gaaaccttta tgacgtcggt ttttatggtt gcgttgtacc
     4921 gcttttttat tctggacgaa aaaccggaca taaaatatat cgagtacaaa aaaggcattc
     4981 ccgaactcaa aacggttttt aagctcccct attattttcg gtttggccgc cgggaatttg
     5041 tgtttgcgct tttttcctcg ttattgggcg tctttttctt attgcttcag gatgttttta
     5101 gactttattg gttgaataac ctggcggctg atgggctttc tggttggata caagctgatt
     5161 taacgcattc tgcattcctt tatacgggca ttgtctattt tttacagttt ttagaacttt
     5221 tggtttatac cgttctggtt ttggtttttc cgtttttggc gctccacgat tcttatgctt
     5281 ttaaaaatat ttctgaaatg gtcaaagctg ttaaagggaa tatctttcgg attttaatta
     5341 tagtgtccct gatatacttg ccctatgatg gttttaattc agctataaat ctggtctatg
     5401 tctatgcttt taagatgggt ttgttatccg attcctctcc ttttagggag gatattcttt
     5461 ttcacgtagg ctggattcgt tattatgcaa gtttaggtgt ttattacgtc tgcgttcttg
     5521 ctgatattat atttttaagc atcatttata aaaggctcgt actcgaaaat gacaaaaatt
     5581 aaagttaaaa atccgattgt tgaaattgat ggcgatgaga tgacgcgcat tatttgggcg
     5641 tttatcaagg agcgtttaat cctgccgtat ctcgatgtcg atttgaaata ttttgacttg
     5701 tcgattcaaa gtcgcgatgc gagcgatgac cagattactg tgatggctgc gaatgctata
     5761 aaagagcatg gcgtgggtgt gaagtgcgca acgattacgc cggacgaggc acgggtggag
     5821 gaatttggcc ttaagaaaat gtggaagtct ccgaacggga caatccgcaa tattttgaac
     5881 gggacggtgt ttcgcgagcc gataaccact gcaaatgtac cgaaatatgt gccgggctgg
     5941 atagagccga ttgtcattgg ccgtcatgct ttcggcgatc agtataaggc cacggacatt
     6001 aaggtgccgg ggccgggtaa gctggagctg gtttatacgc ccgaaggcgg agagccggaa
     6061 gtgcatgagg tgtttgattt tccatccagc ggcgtgggga tggcgatgta taatctggac
     6121 gaaagcattg aaggctttgc aagaagctct tttaattatg ggcttagccg aaaatatcca
     6181 gtttatatgt caacgaaaaa tacgatcctg aaacaatatg acgggaaatt catcgagatt
     6241 ttccagcaaa tttttgatga ggaatttaaa gccgattttg agaagcatga tttgtggtac
     6301 gaacaccgtt taattgatga catggtggcg caggcgatta aatccaaggg cgggtttgtc
     6361 tgggcgtgca agaattatga cggagacgta cagagcgata ttgtcgcgca gggatttgga
     6421 tcactggggt tgatgacttc tgttttgctg acaccggatg ggaaatgcgt tgaggcggaa
     6481 gcggcgcacg gaacagtgac gcgccattac cgcgaacatc aaaaaggcaa tccgacttcg
     6541 accaatccga ttgcttcgat ttttgcctgg acgcgcggac tcaaatatcg cggggaattt
     6601 gacgggacgc cggaggtcgt tgattttgcc gatacgctgg agcgggtttg tgtggaaact
     6661 gttgaagccg ggcacatgac caaggatctg gcgctcttga ttggtgagca acaaaaatgg
     6721 ctgacgacac aggaatatct tgataaggtt gtggctggat tagaaaaggc aatgcaataa
     6781 atgcgtacgg tgtacgtttt tcaaattctt gtttctgtat ttttggctct ggtcagtgcg
     6841 caggcttatg caggctatga taagccgtat gtcggcgaca tgattgaata tgatgcgaag
     6901 tatgaagata cgtttattca tttggcgcgg gattataatc tgggttttac agagcttcgt
     6961 gcggctaacc cttatgtgga tccgtggatg ccggggcgag gcactgattt aattttgcca
     7021 acgcggcatt tgctgcccga tgcgtcgaga gagggcatcg tgattaatct ggcagatatg
     7081 cggctgtatg cttttatcaa tggtgatgaa gcgccttacc atacgccaat tggtgtgggg
     7141 cgtgagggtc ttagtacgcc ggaaggggta accgaaattg tgcgcaaagt ggaagggccg
     7201 gtctggcgtc cgacgccgcg gatgcgcaag gcagatccga cattaaaaga ggcttattat
     7261 cagggaccgg ataatccgat gggtacgcat gcgctgtatt taggatggcc gcaatatgca
     7321 ctgcatggaa ccaaccggcc attcggaatt gggcggcggg ttagttctgg ctgcattcgt
     7381 ttatatccgg aaaaaatatc cgagttattt gagcttatac ctgtgggcac gaaggtaaca
     7441 gtggttaatc agccgataaa agtcgcctgg attgatgatg aactattttt ggaggctaac
     7501 ccttctttgg cgcagtcagt ggcgatggaa gaatatggcg aagtcccgga agagaaaatc
     7561 agtaatgatg agatgcgttt gattgttaaa acggcaggag actggcaaga tcggttgcgt
     7621 tggcctgcga ttcgcaaggc gctgaaagag cgtaatagct atccggtttc gattgcccgc
     7681 cgcccttcac tggaagttaa gggcgatgtt attattgaac atgagaaggc tgcattcatt
     7741 gaaacacctg aagaagcaaa ggatcttatt gaagggtatg agcccgcacc tgaaaaacgc
     7801 gatgatatag aaaaggcgca ggataaaaat tccaagactg gtgaagatac agataagatt
     7861 cggatggctg agggtgagac gacagagcag gatcaggatc aggatcagga taatcccgcc
     7921 agaatgctta atccatagag tagcggtaac agcgctggaa aaagcaggaa tatcagccgt
     7981 aatttgcttt ttacgagaaa aaaccccaga gaaaataatg cgcaaaaaat ccatgttatc
     8041 gcggtggcgg tgaagaaatt tgtctgcagc gttactgcat tggtctcgag cacttcggaa
     8101 gataggtttc ctgaaagcaa cgcagcgtta taggctattg gcgcggccat aaagcccaag
     8161 ccgtttgcga gaagaaataa gacgatctta cggataatgt tcatgtggct attctacttt
     8221 aagaacgtct gtttagcttt ttctacagct catcttacat agagtttctg caaagaaaaa
     8281 gggccgcctt aaggccgccc ttaaatcttt ttatcagctt aatataaagc ttattttgtt
     8341 tggtattctc tgaatacgcg ctctgcaggg gccggagccg gagcaggggc aggttcggat
     8401 gcataaccgg ctgtgcgctc acttgcgtaa ggaggagccg tgtcaacatc acccatttta
     8461 gttgtatcac atgcggacag agcaatagct gttccggcga tgcaaagagt tgctgcaaat
     8521 tttttcattg tgacctcttt aaaattttaa tgttaaacca agaaaaacta tgccctgtaa
     8581 tattgagcag tcgcttcgta gtctctcttt atacacaaac gaaaacgcat tgcacactgt
     8641 ttttcaagag tttttttgtt tttctgggtg gatggtggcg gatttttgaa agattaatac
     8701 attataaaaa atatataatt tcgacagatt cgttcaacga tgcctatatc ctctggtttt
     8761 tgcgaacttg ttcaaaatag cgatcaatgc ctttcgtgag cgcggtggca attttttgtc
     8821 ggtggtggct ggtattaagt aaagtcgctt ctttttcgtt agacataaat cctgcttcga
     8881 tcagcacaga aggaatatcc ggggctttta aaacggcaaa gccggcatag cggtgtgggc
     8941 ggggcagcgt ttttacgcca ttggcgttaa agctgttcac cagggtattg gcaaaaaatt
     9001 ttgactgatt catcgtatcg cgcatagcca gatccataag gatgcccaca acatcctcat
     9061 cttcgatgct aagatctatt cctgcgatga gatcggcctt attttcccgg gcggcgagct
     9121 tggcggtctg ggcatcggag gctttttcgg acagagtata gaccgaagca cctgtaatac
     9181 ttttattttc cattgaatcg gcatgcagtg agatgaagag gtcacctcca tgtttgcggg
     9241 caaatttgac gcggtttgag agcttgatga agtgattgtc tgcgcgtgtc attttaacac
     9301 ggtatttgcc ggaggattca agctgaactt tgaggtcttt agccagagcg agcacaacat
     9361 ttttttcgta caggccgtgc gggccggagg caccgggatc aatgccgcca tggccgggat
     9421 cgatgatgat aagaggtttt tggtcttgtg gtttgggcgg cggcggcggg gctaaagtcg
     9481 caatatttgc attttgtgtt tggattctat caggatcaag agagccatga acagtgcttt
     9541 tgatttgctc aaacaaactg ggcgaaacat tgctgaaatc aatgacaagc cggtcaggtt
     9601 tgcctgcgcc gccaggcagc gtgaaggctt tttgaatgct gatcgggcga ttgaggtcaa
     9661 agacaatgcg tgaaatgccg ggtttgaggc tgccatggcg ggtagcttgt acgcccgcgt
     9721 cttgcgggga ttggactttt ccggcttgcc attcaaagct gggcaggtcg atgaccatac
     9781 gatagggctc agacaagacg aaggcgcgga aatcggctgg cttgtcaaga tccagcacaa
     9841 gcctggtttt ttcttcgtaa aggccaaagc gtacggattc tatattgaga gcacgcgcat
     9901 ttaacggcga aagcagagtc aatagcaaga tcattagagc aaaatggaag ggtttcaatg
     9961 ccaagatagt tactcattga ttgtgttaaa tatgcgcttt cttatatatc ataaatgggc
    10021 gtgggtgtgt aaagctctta atctcgtaag caaggcttgc cgcctgtggg aagagcgcta
    10081 ttatggtgga gaaattttta gcatttatac ttgttttaat gcttgtgtaa tttacccggc
    10141 gcgtgtactg tgcgcttaag tgtttagtgg attgcggttt tagcgcttaa agtttttaaa
    10201 ggtttcgcga actgcactcc ccaaaattga tacaacaagt ttagcgcgct tttgcgccgc
    10261 tttcccctat gtaaacactc gaagtttatc ttcagcggag aaagaggaag cgttcttatg
    10321 cgcgtttaat ggagaataaa taatgtctaa gaaaatgttg attgatggga cccacagtga
    10381 agaaacgcgg gtggccattg ttgaagatgg acggctggaa gagtttgatt atgaaagcgc
    10441 gttccgtaaa ccgttgaaag gaaacatcta tctggcgaaa gtgacacgcg ttgagccatc
    10501 gctgcaagct gcgtttgtaa attttggcgg caatcgccat gggtttttgc cgttttctga
    10561 aattcatcct gattattttc gtattccggt gtcggatcgc gaggcgttga tcgccgaaca
    10621 ggaagcagat ctggaagcac atgatgaaga agaggcgctg gaagaacagg aattgcctga
    10681 agacgatgtt gatgaagaag acgctgttga agagctgggc ggcgatttaa ctcttgagga
    10741 tggcgataag gatggcgagt cggaggaaga agacgttaaa gctgagaaaa aaccgcgcgg
    10801 gggtcgtaaa tctaaaaaag acactgaggt agacactgcc gatgctgtgg atgccgtgga
    10861 ggaagatccc gaggattcaa ttggtaaccg gattgaagag ccggaggcag atattgatgg
    10921 taacaacgcg gatgcacagg gtgaagatgc cgatggcaac atgtatggtg gacgtgggcg
    10981 cggacgtggg cgcggcagac gtgataatgg gcgcggacgt ggcggacgta gccgcaatgc
    11041 agcgcaccgg gccaggcgcg tagaaacttt tggcggcgag gatatg
//
LOCUS       DAOSWR010000065        11026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3762_length_11026_cov_4.970559, whole genome shotgun sequence.
ACCESSION   DAOSWR010000065 DAOSWR010000000
VERSION     DAOSWR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3762_length_11026_cov_4.970559"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..1126)
                     /locus_tag="PK513_06085"
     CDS             complement(305..1126)
                     /locus_tag="PK513_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006014073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOO82051.1"
                     /translation="MSAKVLVLAGYGLNCEEETLHGFEHAGLSGQIRHINDLIENPKE
                     LETAEILAIPGGFSYGDDTGAGNAYAQKLKLALWEPLQKFIARDTLAIGICNGCQILT
                     NLGLNPPTGTPYGERKIAVTYNETARYQCRWIDLKITSSSPWLSGIEHMHLPVAHGEG
                     RFMMDEGTLKTLQNNSQIAAQYIKPGGTPANGEFPYNPNGSLSDIAAITDETERILTI
                     MPHPERGMFTSQRDDYMQIKDAARRKGQAVNETADGMALFTNAARYFEIAQKKSA"
     gene            complement(1126..4020)
                     /locus_tag="PK513_06090"
     CDS             complement(1126..4020)
                     /locus_tag="PK513_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO82052.1"
                     /translation="MTTRIEVLASPEDGRAKTLEKILKHQGKTAQVRVVDVYTSEHPA
                     AANDSFQSSLANPVTQKIGSLPDDFDWALEIGYLPGVTDNVGHTAAELLKLAGAENDN
                     ECFSSRLYLIKGDLKQSDIEELSQSLSNNLIQRASIKSAEKFKADGGMDAVIPRVTLD
                     STSSAAADAIDLNDLDDEQLMEISQKGIQNRGPLGLPLVYMKAIQKHYNGKTITDIEL
                     ETIAQTWSEHCKHTIFASPIDDIADGLYKGFIKRATKEIRAAKGDNDICVSVFSDNAG
                     GIIFDDEYLITDKVETHNSPSALDPFGGAITGIVGVNRDCIGFGQGAKPIINRYGFCL
                     ADPRTSSEYYRDQKCTIPVLSPETIMKGVIDGVEAGGNQSGIPTPQGFVTFDDRFVGK
                     PLVFVGTIGLIPREINGKPGHEKCANPGDKIVVAGGRVGRDGIHGATFSSVALDEGSP
                     ATAVQIGDPITQKKMSDAIIKEIRDMGLYSAITDNGAGGMSSSIGEMAEGSGGFHVEL
                     DKVPLKYPGMAPWEIWISESQERMTMAVPPENVDKIIELFAKRGVEATVVGIYTDDGR
                     AKITYQGETIMDMDMDFLHDGNPETPLATNFTRGGNDEPDINEPSAHESALLNMLGRL
                     NICSKEFISTQYDHNVQGSAVMGPLQGPGRVYAETSITRPVLTSNKGIVLSQGLFPRY
                     SDVDTAAMAAAGLDLAVRNAVAAGVDLNQLALLDNFCWCSSDEPERLGQLKRAVETIY
                     EMAVKYETPFISGKDSMFNDFKGFDKNGKPVKISAPPTLLVSSIGVIPDIERSIDLAP
                     KAVGDLVYLLGETKDECGASEYYDHLGHLGNNVPETNSHQNLRLYKLYGRAARDRLIA
                     SAYALNYGGLGVALAKKAIAGQMGMDIDLSTIDSELRSDKLLYSESTGRILVTVAPQD
                     KDKFEKNFKGYEHCTFVGHIAYGNKLNIKNILQVDIDKLDEAYKAPLRNC"
     gene            4116..6200
                     /gene="nuoG"
                     /locus_tag="PK513_06095"
     CDS             4116..6200
                     /gene="nuoG"
                     /locus_tag="PK513_06095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOO82053.1"
                     /translation="MATVKLTINDMEIEVERGTSILQAAEALGIEVPRFCYHDKLSVP
                     ANCRMCLVEVEGGPPKPVASCAMAAGEGMVVHTDSEMVKKARKGVMEMLLINHPLDCP
                     ICDQGGECDLQDQAVGYGFDRSRFAENKRAVKDKELGPLISTTMTRCINCTRCVRFAE
                     EIAGTPVLGQLNRGEDAEIGTFIGELVQTELSGNLIDVCPVGALTSKPYAFKARPWEL
                     KKVETIDVHDAVGSNIRVDVRGREVMRILPRLHDDVNEEWISDRTRFAYDGLNNGRLD
                     RPYIRDEDSGKLVEASWEAAFALIAKKLSSLKGEQIAGLVGDLAEVESIVALKDLLEK
                     LGSPNMDCRTDGAQFDPSNRAGYLFNSTIAGIDESDAILLIGSNPRHEATVINARIRK
                     AQFERRVKIGLIGERAALTYDYEHFGDGPEALEKFVKAKSGFAKVFKDAKKPMIVVGS
                     AVFARPDGEAIHHALYMAAEKLGVAKEGWNGFNILHRAASRVGALDVGFVPQVGGKDF
                     AGIVKGTKDGSIKALYMLGADEFDARASIGWKTFVIYQGHHGDHGALRADVVLPGAAY
                     TEKDGIYLNTEGRVQYARRASFPPGDAKEDWKILRALSEALGQTLPYNTQAELRDRVL
                     DEFKHMSVVDSIAPAPWGEFGTKGPADKASFVNPVRNYYMANVICRASATMRDCVESF
                     THADDEMLEAAE"
     gene            6296..6700
                     /locus_tag="PK513_06100"
     CDS             6296..6700
                     /locus_tag="PK513_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOO82054.1"
                     /translation="MSKTTLEYVQIFHETYGLPVKAAPDISDEKTNALRVNLLAEELE
                     ELQEALAEGNIVEVLDALTDIQYVLDGAYLSFGLHGVKTAAFEEVQRSNMSKLGADGK
                     PIVREEDGKILKGPNYFKPDIAQFIEGKDKAA"
     gene            complement(6697..7932)
                     /locus_tag="PK513_06105"
     CDS             complement(6697..7932)
                     /locus_tag="PK513_06105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO82055.1"
                     /translation="MADKTEKRSILDDILAHVTVQSANGAISINSSSLGKGKSQTVKN
                     LQQELSNEGLYVSKGHRYYNDGYPGGITDFGLLMKADPIQAINHINDALNDNNLNEKD
                     ILKLQIALNASTDDPTKKIKVLGEMNAQTANRLADHLAQQMIKPDPNSALSKALQKHA
                     APTKLSEIGIEAKKQTSDRFNNSAAHHNHDHGHGHGHGQNKEIQTRKPVGSNTLDTST
                     SPLTLRTPINNMRVTSTLKERFGKSHQGLDINTEKRGNANIGEPIHAPANGVVAYAGA
                     SSGYGKLISLYHGHNVYTQYGHVRDTNHLSIGQSVNTGENFAQIGKSGTNIAHLHFEV
                     IIERNGVGYIVDPQKALVPGVNLYDPDVQNKLIESASEKISKNTSEAFGTRVASFNEQ
                     RYETTLIASNTINDKPAIT"
     gene            8129..9142
                     /gene="nuoH"
                     /locus_tag="PK513_06110"
     CDS             8129..9142
                     /gene="nuoH"
                     /locus_tag="PK513_06110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOO82056.1"
                     /translation="MFVLFENLYVLWTALTLLYTVLIVAGVLMAVAYYTYAERKIMGA
                     MQRRQGPMTVGPFGLLQPIADGIKLFSKETIIPSGANRPVFLLAPMMLFALSLIAWAV
                     IPFDKGWVLANINVGILYLFAVSSMTVYGVIMAGWASNSRYAFLGGLRSASQMVSYEV
                     SMGLIIVCVLLCTGSLNLQEIVEAPRPLWMQVLLFPMLIVFLVSILAETNRAPFDLPE
                     GESEITGGFMVEYSAMTYALFFLGEYSAMILMSAMTTTLFLGGWLSPFGMSFLAFVPG
                     IIWFAMKTALVMFFFIWARATLPRFRYDQLMRLGWKVFLPLTLVWVVFTAGVLITFDA
                     LPG"
     gene            9153..9635
                     /gene="nuoI"
                     /locus_tag="PK513_06115"
     CDS             9153..9635
                     /gene="nuoI"
                     /locus_tag="PK513_06115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOO82057.1"
                     /translation="MDGFARSFLLTEIVKGFLLTLKYMFFVPRVTINYPYEKGPISPR
                     FRGEHALRRYPNGEERCIACKLCEAICPALAITIETEPRADGSRRTTKYDIDMTKCIF
                     CGLCQEACPVDAIVEGPNFEYSTETREELFYNKDKLLANGDRWEAQIAANIAADAPYR
                     "
     gene            9675..10259
                     /locus_tag="PK513_06120"
     CDS             9675..10259
                     /locus_tag="PK513_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82058.1"
                     /translation="MALKRVVFDDRRLATMFGDQSQAGGVYSSALLVGGLCCEDDCLQ
                     DSDTVALVQSVKWINQNLDVCIAANPKVDVCNLDYGSNFFELGSEDNYDFVVFCNLYF
                     SGLGNPEHSFFSLSRQSELAFDRENWKKSIEHISPKAILNCHVVGEPEGQAINDVLAS
                     IIPYVSDSELYRGTTITKQKISGFAFDALRIPEV"
     gene            complement(10268..10771)
                     /locus_tag="PK513_06125"
     CDS             complement(10268..10771)
                     /locus_tag="PK513_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOO82059.1"
                     /translation="MRRKALSAFLSAGLLSAITPAAATDTAPAYWLNFAIGYYDVFDE
                     QDGFDLRAEFRPNSTVFIDNLKPWAGLELTSQGSIWAGIGLLYDRNFKENWYFTPSLG
                     VGFYTDGGSDKDLDYPVQFRSQLEIDYELESQHRIGLSLSHMSNAGLGDHNPGTEVIS
                     LNWSYPF"
BASE COUNT         2347 a         2214 c         3251 g         3214 t
ORIGIN      
        1 aacaacaatg tagtgagggg tcggaggttt acggccgggg cggttttaag cgttattgtg
       61 gctggggggg actgcatatt cggctgttga tgattttcga aaggctgtgg tttccaacga
      121 tcatcaggcc agaagcgctt tagtgagcgg tgtcgttaag ggtataggcg gcgttggttt
      181 tcttgggctt tcagggtttt ttggattttg ggcaaagcgt cattatgggc gcgcgcgaaa
      241 gtataagaaa cttctctcta gtgctactga acttactcct cggcaggatt gatttgatta
      301 aacgttaagc gctttttttc tgcgctattt cgaagtagcg cgcggcgttg gtgaacaaag
      361 ccatgccgtc ggcggtttcg tttactgctt ggcctttgcg gcgggcggca tccttgattt
      421 gcatataatc atcgcgctgg ctggtgaaca tgccgcgttc ggggtggggc atgatggtta
      481 aaatgcgctc ggtttcatcg gtgatggcgg cgatatcgct gagcgagccg ttggggttgt
      541 aggggaattc gccattggcg ggcgtgccgc ccggtttgat atattgcgcg gcgatctggc
      601 tgttattctg gagggttttg agggtgcctt catccatcat aaagcggcct tcgccgtggg
      661 cgacggggag gtgcatgtgc tctataccgc tgagccatgg ggagcttgag gtgattttca
      721 ggtcgatcca gcggcattga tagcgcgcag tttcattgta tgttacggca atcttgcgct
      781 cgccataggg ggtgcccgtg ggcgggttga ggccgagatt ggtgagaatc tgacagccgt
      841 tgcatatgcc gatggccagt gtgtcgcgcg cgatgaattt ttgcaaaggt tcccacaatg
      901 cgagtttgag tttttgcgca taggcgttgc cggcgccggt gtcatcgccg taggaaaacc
      961 cgccggggat agcaagaatt tctgccgttt ccagctcctt tgggttttcg atcagatcgt
     1021 taatgtggcg gatttggcct gagaggcctg catgttcaaa accgtgcagg gtttcttctt
     1081 cacagttaag gccgtatccg gcgagtacga gaactttagc gctcattagc aattccttag
     1141 gggcgcttta tacgcctcat ccagtttatc aatatcaact tgtaatatat tctttatatt
     1201 gagtttattg ccgtaggcaa tatgacctac aaacgtgcaa tgctcataac ctttaaaatt
     1261 tttctcgaac ttgtccttgt cttgcggggc gacggtgacg aggatgcggc cggtgctttc
     1321 ggagtacaga agtttgtcgc tgcgcagttc gctgtcgata gtcgacaggt caatatccat
     1381 gcccatttgc ccggcgatgg cttttttggc caaagctacg ccaaggccgc catagttcag
     1441 cgcgtaggcg gaggcgatca ggcggtcgcg ggcggcgcgg ccatagagtt tatagaggcg
     1501 taagttctgg tggctgttgg tttctgggac gttgttgccg agatggccga gatggtcgta
     1561 atattcggat gctccgcact catccttggt ttcgccgagc aggtagacca gatcgccaac
     1621 ggctttggga gcgagatcga tggaacgctc aatatcggga atcacaccga tggaagaaac
     1681 cagcaaggtt ggcggggccg agattttgac tggtttaccg tttttatcaa agcctttgaa
     1741 gtcgttgaac attgagtctt tgccggaaat gaacggggtt tcgtatttta cggccatctc
     1801 ataaattgtt tcaacggcgc gtttgagctg ccccaagcgt tcgggttcat ctgagctgca
     1861 ccagcagaaa ttgtcgagca gggcgagttg gttcagatcc acaccggcgg caaccgcgtt
     1921 acgtacggcg agatcgaggc ctgcggcggc catggcggca gtgtcgacgt cgctgtagcg
     1981 cgggaagagg ccttgggaga ggacaatgcc tttgttgctg gttaaaacag gccgagtgat
     2041 tgaggtttca gcgtaaacgc gtcctgggcc ttgtaacggt cccataacgg cggagccctg
     2101 cacgttatgg tcatattggg ttgagatgaa ttcttttgaa cagatattca aacggcccag
     2161 catattcagc aatgcgcttt catgggcgct gggttcatta atatccggtt catcattacc
     2221 gccgcgggtg aaattggttg caagcggggt ttcaggattg ccgtcatgga ggaagtccat
     2281 atccatatcc atgatggttt cgccttgata ggtgattttt gcgcggccat catcggtgta
     2341 gatgccaaca acggttgctt caacgccccg cttagcaaag agttcaatga ttttatcaac
     2401 attttcgggc ggtacggcca tggtcatgcg ttcttgtgat tcgctgatcc agatttccca
     2461 tggggccata ccagggtatt ttagcggtac tttatccagt tcaacatgga agccgccgga
     2521 gccttcagcc atttcaccga tggaagaaga catgccgcct gcgccgttat cggtaatcgc
     2581 gctgtagagc cccatatcac ggatttcttt gataatagcg tcggacattt ttttctgcgt
     2641 gatggggtcg ccgatttgga cggcggtggc aggggagcct tcatcgagtg ccacggaaga
     2701 aaatgttgcg ccgtggatgc cgtcgcggcc aactctgccg cctgcgacaa cgattttatc
     2761 gccggggttt gcgcattttt cgtggccagg tttcccgttg atttcgcgcg ggatgaggcc
     2821 gattgttccc acgaaaacca aaggctttcc gacgaagcga tcatcaaagg tcacaaagcc
     2881 ttgcggggtg gggatgccgg actggttgcc gcctgcttct acaccgtcaa tcacgccttt
     2941 catgatagtt tcaggggata gcacggggat tgtgcatttt tgatcacggt aatattccga
     3001 cgaagtgcgc gggtcggcga ggcaaaatcc gtaacgattg atgataggtt tggccccttg
     3061 gccgaagccg atgcagtcgc ggttgacgcc gacgattccg gtgattgcgc cgccgaacgg
     3121 gtcgagggcg gaaggggagt tgtgcgtttc aaccttgtcg gtgatgaggt attcatcatc
     3181 gaaaataatg ccgccggcgt tatcggaaaa gaccgaaacg caaatgtcat tatcgccttt
     3241 agcggcgcgg atttctttgg tggcgcgctt gataaagcct ttgtaaagac cgtcggcaat
     3301 atcgtcgata ggggaggcaa aaatcgtgtg tttgcagtgc tcggaccatg tttgcgcaat
     3361 ggtttcgagt tcgatgtctg tgatggtttt gccgttatag tgcttttgta tggctttcat
     3421 ataaaccaga gggagaccga gggggcctcg attttggatt cccttttggc tgatctccat
     3481 gagttgttcg tcatccaaat cattgaggtc aattgcgtcg gctgctgcgc tgcttgttga
     3541 atcaagcgtg acgcgaggga tgacggcgtc cattccgcca tcggctttga atttttcagc
     3601 gcttttgatg gaagcacgtt gaatcagatt gttggaaagg ctttgcgaaa gttcttcgat
     3661 atcgctttgc ttaaggtcgc ctttgatgag atataggcgg gaagagaagc actcattgtc
     3721 gttttcggct ccggccagtt ttagcaattc tgcggcggtg tggccgacat tgtcggtaac
     3781 gccgggcaga tatccgattt ccagggccca gtcgaaatca tcggggaggg agccgatttt
     3841 ctgagtcaca gggttggcaa ggctggattg aaagctgtca ttggcggcgg ccgggtgttc
     3901 gctggtgtaa acatcgacca cgcgcacttg cgcggttttg ccttggtgct ttaagatttt
     3961 ttccagtgtt tttgcgcgcc cgtcttccgg tgaggctaga acttcaatgc gagtggtcat
     4021 atacttttaa actaactgtt gatcttggct ttcgcgcaga ttaaacagga ataggttaga
     4081 gaattcaata cacttcacaa cagaaactca aatttatggc cacagttaag ctcacaatca
     4141 atgacatgga aatcgaagtc gaacgcggaa caagtatttt gcaggcggcg gaagcgctcg
     4201 gcattgaggt gccgcgcttt tgttatcatg acaagctgag tgtgccggca aattgccgga
     4261 tgtgtctggt ggaggttgaa ggcgggccgc caaaaccggt ggcatcatgt gcgatggctg
     4321 cgggtgaagg catggttgtg cataccgatt ccgagatggt taagaaggcg cgcaagggcg
     4381 tgatggagat gcttctcatc aatcatccgc tcgattgtcc gatttgtgat caggggggcg
     4441 aatgtgattt acaggatcaa gctgtaggct atggatttga ccggagtcgt tttgcggaaa
     4501 ataaacgtgc ggtgaaggac aaggagctag ggccgctgat tagcactacg atgacgcgct
     4561 gcattaattg tacgcgctgt gtgcgttttg ccgaagaaat cgccggtacg cctgtgttgg
     4621 ggcagcttaa tcgcggggaa gatgccgaga ttggtacatt tattggtgaa ttggtgcaga
     4681 cagagctttc gggcaatttg attgatgtgt gcccggtagg ggcgctgact tccaagccgt
     4741 atgcatttaa ggcgcggcca tgggagttga aaaaggttga gacgattgat gtgcatgatg
     4801 cggtgggatc gaatatccgc gttgatgtgc gtgggcgaga agtgatgcga attctaccgc
     4861 gcttgcatga cgatgtgaat gaggagtgga tttctgaccg gacgcgcttt gcgtatgacg
     4921 gccttaataa cgggcgtctg gataggccat atattcgtga tgaagacagt ggaaagttgg
     4981 ttgaggcgag ttgggaagcg gcgtttgcgt tgattgccaa aaaactttca agtttgaagg
     5041 gtgagcagat tgccgggctg gtcggtgatt tggccgaagt cgaatcgatt gtcgcgttga
     5101 aagatttact cgaaaaactg ggcagtccga atatggattg ccgtactgac ggggcgcagt
     5161 ttgatccatc gaaccgggcg ggatatttgt ttaattctac cattgccggc attgatgaat
     5221 ctgatgcgat tttgctgatc gggagcaatc cgcgtcatga agcgacggtt attaatgcgc
     5281 gtattcgtaa ggcacagttt gaacgccggg tgaagatcgg gttgatcgga gagcgggcgg
     5341 cgttgaccta tgattatgag cattttgggg atgggcctga ggcactcgaa aagtttgtga
     5401 aggctaaaag cggttttgca aaggtcttca aagacgccaa gaagccgatg attgtcgttg
     5461 gcagcgccgt gtttgcacgg cctgacggcg aggcgattca ccatgcgctt tacatggctg
     5521 ccgaaaaact tggcgttgcg aaagagggtt ggaacggctt taacattttg caccgcgcag
     5581 cgagccgggt tggggcgctt gatgtgggat tcgttccgca ggttggcggt aaggattttg
     5641 ctggaattgt taaggggacg aaggatggct ctatcaaagc tctgtatatg ctgggcgcgg
     5701 atgagttcga tgcgcgtgcg tctatcggct ggaagacttt tgtgatttat caagggcatc
     5761 acggggatca tggggcgctt cgtgcagatg tggttttgcc gggggctgct tataccgaaa
     5821 aagacgggat ttatctaaat accgaagggc gggtgcaata tgcgcgccgc gcttcgttcc
     5881 cgccgggtga tgccaaagaa gactggaaga ttttgcgggc gctttcggaa gctttaggcc
     5941 agacattgcc gtataatacg caggctgagt tgcgggatcg tgttttggat gaatttaagc
     6001 atatgtcagt tgtcgattct attgcaccgg cgccgtgggg tgaatttggc acgaagggcc
     6061 cggccgataa agcttctttt gtgaatcctg tacgtaatta ttatatggcg aatgtgattt
     6121 gccgggcttc ggcgacgatg cgagactgtg tagaatcgtt tactcatgct gatgatgaaa
     6181 tgttggaggc ggcggagtga tgacaatgtt tgcgagtatg tcattgcgag gacgatgcag
     6241 caatctagtt tatggattgc ttcgctgcgc tcgcaatgac gaaagaggat aggatatgag
     6301 caaaacgaca cttgaatatg tacagatttt ccatgaaacg tacgggcttc cggtgaaggc
     6361 tgcgccggat atttcggatg aaaagaccaa tgcgctgcgg gtgaatttgc tggccgaaga
     6421 gcttgaggaa ttgcaggagg cgctggcgga aggaaatatt gtcgaggtac ttgacgcgct
     6481 caccgatatt caatatgtgc tggacggggc gtatttgtct tttggtttgc atggtgtgaa
     6541 aacggcggca tttgaagagg tgcagcgctc gaatatgagc aagcttggtg cagatggcaa
     6601 gccgattgtg cgcgaagagg atggcaagat tctcaaggga ccgaattatt tcaagcccga
     6661 cattgcgcag tttattgaag ggaaagataa agcggcttaa gtgattgccg gtttatcgtt
     6721 gattgtattt gatgcgatga gtgtcgtttc gtagcgttgt tcattgaaac ttgcgactct
     6781 ggttccgaag gcctctgaag tgttctttga gattttttcg ctcgcgcttt caataagttt
     6841 gttttgtacg tcagggtcat aaagattaac acctggtact agggcctttt gcggatcgac
     6901 aatgtagcct acaccgtttc tttcgatgat cacttcaaaa tgtaaatggg caatgtttgt
     6961 gccggatttg ccgatttgcg cgaaattttc gccggtgtta acagattgac cgatcgaaag
     7021 gtggtttgtg tcccggacat ggccatattg cgtgtatacg ttgtggccgt ggtacaagga
     7081 tatcagttta ccatagccgc tggaggctcc tgcataggcg acgacgccgt ttgcgggggc
     7141 gtgaattggc tcaccgatat tcgcattacc tcttttttcg gtatttatat ccaagccttg
     7201 atgtgatttg ccaaaccttt ctttgagagt gcttgttacc ctcatattgt ttattggcgt
     7261 tcgcaatgtc aaaggactgg tgcttgtatc cagtgtattg cttccgaccg gttttcgggt
     7321 ttgaatctct ttattctgcc catgcccatg cccatgccca tgatcgtgat tgtgatgtgc
     7381 ggcgctgttg ttaaagcgat cagaggtttg tttttttgcc tctattccta tttcacttaa
     7441 ttttgttgga gcggcgtgtt tttgcagggc tttgctgaga gcgctgtttg gatctggttt
     7501 tatcatctgt tgcgcgaggt gatcggcaag gcggttggcg gtttgtgcgt tcatttcgcc
     7561 gagaactttg attttttttg tcggatcgtc tgtgctggca ttcagcgcta tttgtagttt
     7621 cagaatatcc ttttcattta aattattgtc gttaagagcg tcattaatgt ggtttatggc
     7681 ctgtattgga tcggccttca ttaacaggcc aaaatcggtg attcctcctg gataaccgtc
     7741 attataatag cgatggcctt tggacacgta cagaccttcg ttggacagct cttgttgtaa
     7801 gtttttgact gtttgtgatt tgcctttacc caagctggag ctgttgatgc tgatagcgcc
     7861 attggcgctt tgcacggtta cgtgtgccaa aatatcatca agaatggatc gtttttcggt
     7921 cttatccgcc attttggtgc ctctattttc ccttatgtat attttacagg ggcttagtgc
     7981 attgtaatta taatatgtta atgaaaagtt aaaattgtgg cattctcgag atttgacgtg
     8041 ggtatagccg ggattttagc gtttactttt tcttttaaag gttgcacatt gggcgaggat
     8101 gttaacgctt aaatttttat aattcctgat gttcgtactt tttgaaaatc tttatgtttt
     8161 gtggacggct ttaacgctgc tttacacggt tttgatcgtg gccggtgtgc ttatggctgt
     8221 ggcgtattat acttatgccg agcgtaaaat tatgggcgcg atgcagcgca gacaggggcc
     8281 gatgacggtc gggccgtttg ggttgttgca gccgattgcc gatggaatta agctgttttc
     8341 caaggaaacg attattccaa gcggggcgaa ccgtccggtg tttttgctcg cgccgatgat
     8401 gctgtttgcc ttgtcgttga ttgcatgggc ggtgattccc tttgataagg gatgggtgct
     8461 ggccaatatc aatgttggga ttttgtatct ttttgccgtg tcgtcgatga cggtgtacgg
     8521 cgtgattatg gccggatggg cgtcgaattc gcgctatgct tttctgggcg gtcttcgcag
     8581 cgcttcgcag atggtgtctt atgaagtttc aatggggctg attatcgtgt gtgtgctttt
     8641 gtgtacgggc agtctcaatt tgcaggaaat tgttgaagca cctcgtccgt tgtggatgca
     8701 ggttttgctg tttccgatgt tgattgtgtt tctggtttct atattggcgg aaacaaaccg
     8761 tgcgccgttt gatttgccgg agggcgagtc ggagattacc ggcgggttta tggttgaata
     8821 ttcggccatg acttatgcgc tgtttttctt gggtgaatat tcggcgatga ttttgatgag
     8881 cgcgatgaca acaacgttat ttcttggtgg ttggctgtca ccatttggaa tgagtttttt
     8941 ggcgttcgtg ccgggaatca tctggtttgc gatgaaaacc gcgttggtga tgtttttctt
     9001 tatctgggca cgggcgacgt tgccgcgttt ccgttatgac cagttgatgc ggctggggtg
     9061 gaaagtgttt ttgccgctta cgcttgtgtg ggtggtgttc accgccgggg ttctcattac
     9121 gtttgatgcg ctgccggggt aaaggaattg atatggacgg atttgcacgc tcatttttgc
     9181 tgacggaaat tgtgaagggg tttttgctga ccctgaaata tatgtttttt gttccgcggg
     9241 taacgattaa ctatccgtat gaaaagggac caatttcccc gcgatttcgc ggtgagcatg
     9301 cgttgcgccg ctatccgaat ggtgaagagc gctgcattgc gtgtaagctg tgcgaagcga
     9361 tttgtccggc tttggccata actattgaaa ccgagccgcg cgcggacgga tcgcgacgga
     9421 cgacgaaata cgatatcgac atgaccaaat gtattttttg cggtttgtgt caggaagcct
     9481 gcccggttga tgcgattgtt gaggggccga attttgaata ttcaactgaa acgcgtgaag
     9541 agttgtttta taacaaagac aaactgcttg ccaatggcga tcgctgggaa gcgcaaattg
     9601 cggcaaatat tgccgcagat gcgccttata gatagtctgt tgacataatt ttatattact
     9661 ttatatttct tttcatggct ttaaagagag ttgtgtttga tgatcggcgg cttgcgacta
     9721 tgtttggtga tcagtcacag gcagggggtg tttattcaag tgctttactt gttggtgggc
     9781 tttgttgtga agatgattgc cttcaagaca gtgatactgt cgctcttgtt cagtcggtga
     9841 aatggattaa tcaaaatcta gatgtttgta ttgcagccaa cccaaaagtg gatgtttgca
     9901 atctcgatta tggttcaaac ttttttgaac ttggttcaga ggataactat gactttgtcg
     9961 ttttttgtaa tctttatttt agtggtctcg gtaatcctga acatagtttt ttttctttat
    10021 cgcgccaaag tgaattggcg tttgatcgcg aaaattggaa aaaatctatc gaacacattt
    10081 ctcctaaagc tattttaaac tgccatgtcg ttggtgagcc ggaaggtcag gctattaatg
    10141 atgtattggc ttcgattatt ccatatgttt cggacagcga actttataga ggcactacta
    10201 taactaaaca aaaaataagt ggttttgcat ttgatgcgct gagaattcca gaagtttaag
    10261 gtggttttta aaacggatag gaccagttca ggctgatgac ttcggtgccg gggttgtgat
    10321 ctccgaggcc tgcgtttgac atgtggctta aggacaggcc gatgcgatgc tggctttcca
    10381 gttcgtaatc aatttcaagt tggctgcgga actggacggg atagtcgagg tctttgtcgc
    10441 tgccgccgtc ggtgtaaaac ccgacgccca ggctgggggt gaagtaccag ttttctttaa
    10501 aattccgatc atagagcagg ccgatgccgg cccagattga gccttgtgag gtgagttcta
    10561 atcctgccca gggtttcaaa ttatcgataa atacggtgct gtttgggcgg aattctgcgc
    10621 gcaggtcgaa gccgtcttgc tcatcaaaca catcatagta accaatggcg aaattgagcc
    10681 agtacgcggg ggctgtatct gtggctgctg cgggggtgat tgcgcttagt aatcctgcgc
    10741 tcaaaaaggc ggagagagct tttcttctca tgggtgattt ctgttttagg gcgggcgtgt
    10801 aaaaggattt aaaagcagtg ctttatcatt tttataatgc ccgattaagg ataaaaatca
    10861 atcttgaaaa aatgtctaaa atgtagagga ttttttgggt taggggcctt gcgcgccgcg
    10921 gcgtaacaga ctagaacgaa agggcagaaa caaaagattg ttcttatgat catttccggc
    10981 attgccttct atatttttgc tgtgattttg acgctgagcg ccttga
//
LOCUS       DAOSWR010000066        10984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3775_length_10984_cov_6.034678, whole genome shotgun sequence.
ACCESSION   DAOSWR010000066 DAOSWR010000000
VERSION     DAOSWR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_10984_cov_6.034678"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK513_06130"
     CDS             complement(<1..554)
                     /locus_tag="PK513_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82060.1"
                     /translation="MADTAEEKRKKDEESITDIMSMLYGGGYAYYRPASFRPAVLTSD
                     DNESPAADAVTPVAVTTGAAGAAVATQQTEPETTETVMELRGSDGQLIALDPAVLEGP
                     LAARHRFASNDPRLTLTKEDIDNGADKALFDFWQEKKDAAKLSFEVDTSLFSGELKED
                     DLLKNKSFEEAAKVLYEMEHGKGAA"
     gene            complement(620..1114)
                     /locus_tag="PK513_06135"
     CDS             complement(620..1114)
                     /locus_tag="PK513_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82061.1"
                     /translation="MGFWLPFLALAGGGWYGANKLSDGALNETAGAFISDSASNLGGK
                     VVDGALDKAGLDDDSAMGNFFKDHWGKTLLGAGGLLGAMKLPKPLRGLAMAAAVVIGL
                     VTIVQHYSSEKLQGDFEVAGADTQELALPNVPSRNNPEYVLQNKNGAFDLGIDFRAPE
                     AEPS"
     gene            complement(1575..3227)
                     /gene="purH"
                     /locus_tag="PK513_06140"
     CDS             complement(1575..3227)
                     /gene="purH"
                     /locus_tag="PK513_06140"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOO82062.1"
                     /translation="MSSSEVSQAAAPNDFQENIIRDPRAVKIKRALISVSNKNGMEDF
                     ARKLEAHGVEILSTGGTAKMLKDAGIAVKDVSEHTGFPEIMDGRIKTLHPSVHGGILA
                     RRSDKAHVQAMEDNDIVDIDMVVVNLYPFMETVQSGKNYDECIENIDIGGPAMIRAAA
                     KNHEFVAVITDPQDYDKVLTDMDHHDGAVTYKTRKALAATAYALTATYDSNIASWFVE
                     QMGDDFGFPRRISFSGTLKQTLRYGENPHQNAALYTVDGSVRPGAARATQTQGKELSY
                     NNLNDTDAAFELVAEFDEPAVAIIKHANPCGVAMADDMLEAYKRAFACDPVSAFGGII
                     ALNRTLTKDTAEAILQKFCEVIIAPNIAADAIEVLKAKKNVRVLTTGGMPNLTQKRRM
                     VTRIAGGLLVQDSDNGQTLEDDLKVVTDREPTKEELKDMLFAFRVAKHVKSNAIVYAK
                     GGATVGIGAGQMSRVDSARIAAWKSAEAARELGLDEPLAKGSVVASDAFFPFADGLIS
                     AAEAGVTAVIQPGGSIRDEEVIEAANERGLAMVMTGMRHFRH"
     gene            complement(3288..4145)
                     /locus_tag="PK513_06145"
     CDS             complement(3288..4145)
                     /locus_tag="PK513_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HOO82063.1"
                     /translation="MKITDFDIYKDLLKEKSGLVLSQDKSYLVESRLNPVAKKWGYDS
                     LEAMTMTLQGVPDKELVRDIVEAMTTNETSFFRDSRPFDLFKDTVLKYMKEARAPRRR
                     LRIWCAAASSGQEPYTIAMLLKEAAMEFPGWSFEIIATDISHDILNLAREGRYTQFEV
                     QRGLPIQLLMKYFTQDGDRWQIKDDIRKMVKYDYFNLLDPMTKLGTFDIIFCRNVLIY
                     FDEQTKGAVLNKMAQQIEKDGFLFLGGAETVLGITDSFKPIPDRRGLYAVEGSPLLKI
                     SQTDTQALA"
     gene            complement(4150..5247)
                     /locus_tag="PK513_06150"
     CDS             complement(4150..5247)
                     /locus_tag="PK513_06150"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOO82064.1"
                     /translation="MSTTSNKKIHIMLVDDSAVIRGALGRIIDPAPDMTVVASIANGE
                     MGIRSAEKYKPDIVILDIEMPVMDGITALPKILEASPNSKVIMFSSLTENGADVTLKA
                     FSLGAVECIVKPSSTQGVGEGSEFQRNLLKIIRGLVPESERRAVDVTNPSASTPPAQT
                     TLKEFSLRSSVGVYPGSPSIIAIGSSTGGPQALFEVIKNFAGIRIPIVITQHMPATFT
                     KILAQHITQHTGCKAHEASDGMRIENGKAYVAPGGFHMKISKEAEGLVARLDNGPPEN
                     FCKPSVEPMFRSVIDIFGNKVYGIILTGMGNDGIGAGKLLTDCGARLVAQDEASSIVW
                     GMPGAVAKAGYCTNVLPLSEIGPYVRKTIAG"
     gene            complement(5286..5663)
                     /locus_tag="PK513_06155"
     CDS             complement(5286..5663)
                     /locus_tag="PK513_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82065.1"
                     /translation="MKSCLIVDDSRVVRKVARRIAEDLGFECDEAEDGQKAYDACQVK
                     MPDAVLLDWNMPIMSGIEFLEKLRQMENGSHPKVVFCTTENDMNHIQRAIQAGANEYI
                     MKPFDSEIIESKFIQIGLLEQSS"
     gene            complement(5748..6236)
                     /locus_tag="PK513_06160"
     CDS             complement(5748..6236)
                     /locus_tag="PK513_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO82066.1"
                     /translation="MSANADALDYEGSENQHDTQSKEFLTIVIAGQRFGIPILQVQDV
                     LGEQTVTKIPLAPPEVAGSLNLRGRIVTAIDMRCRLGIEPCSKTTSSTMGVVVEHENE
                     LYSLIIDEVGDVLRLRNESFESTPSTLDSLWREISSGVYRLDDELLVILDVPNFVSSV
                     HS"
     gene            complement(6233..9103)
                     /locus_tag="PK513_06165"
     CDS             complement(6233..9103)
                     /locus_tag="PK513_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO82067.1"
                     /translation="MDDLINEFLTETNESLDELDLDLVNLEQNPNDKDLLSKIFRLMH
                     TIKGTCGFLGLPRLEKVAHHAENVLGRFRDGDLEVTPDYVTLILESIDRIKLIVGGLE
                     ETGSEPEGDDSALIEKLDAVYEGRDGGGAASSGAEVSPKEAVETPLEGGNCLTQEELD
                     ALEAAFQNAEGPEEFALKTVGEEPGADAPSAPEEATVIKEEPPKAAAPANDASVAKTA
                     EKPVSAQSLRVSVDVLENLMTMVSEMVLTRNQLIQILRQNSDSEFSVPLQRLNHVVSE
                     LQEGVMQTRMQPIGNAWSKLPRIIRDLAIEVNKKIELEMKGQDTELDRQVLELIKDPL
                     THMVRNSGDHGIEDPETRKAAGKPETGKILLNAFHEGGHIIIQISDDGKGLPLDKIKA
                     KVLENGLATAEELEEMSSNQINQFIFNAGFSTAEKVTAVSGRGVGMDVVRTNIEKIGG
                     SIDLQSMEGKGSTFTIKIPLTLAIVSALIVESAQERFAIPQLAVRELVMASEDGENRI
                     ETIKGTPVFRLRDRLLPLISLSDLLKLSPPDKKVVADASQESNSAESDDTPPEDSSPK
                     IDSVSNPKKRHQYIVVTQVGNNDFGIIVDRVFDTEEIVVKPVAKILRNIEMFSGNTIL
                     GDGSVIMILDPNGIAKHIGQVDATDGARTLDQDAAIDSARGEKTSLLLFTAGNKTPKA
                     VPLSLVARLENIDVSNIERSAGQMMVQYRGKLMPLMPLDPRVQIGETGEKPVLVFADK
                     GRSLGIIVDEIIDITEEHINLQLEGGQGGMMGSAIISGKATDIVDVGHFLQANGSDWF
                     KDHDDTPYGSSKDTTNGKNNGHAKKRVLLVDDSPFFRNMLTPLLSVAGYDVISLESPH
                     DALKMCDKGAEFDVIISDIEMPDMDGFEFAQKVKSETNWKETPMLALSSHATPQDIDR
                     GMSVGFSKYIAKFDRDTLLNTLSQTIAEQQQNYDLTKQGDVA"
     gene            complement(9361..10005)
                     /locus_tag="PK513_06170"
     CDS             complement(9361..10005)
                     /locus_tag="PK513_06170"
                     /inference="COORDINATES: protein motif:HMM:NF021595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HOO82068.1"
                     /translation="MSVTIDTDILELMASKICHDLISPIGAVNNGIEFLTEMGPDAGE
                     EVTDLIAFSASQASAKLKAYRMAYGAGGADDSIKPEEVHRAIEDIVGAEKKIVQEWDP
                     YAPLGYEDRPKAFCKMLICALLLVMDCLPKGGILKVEAGENNGSTLVSGSGQDAGIRE
                     QTAQALDLSMSRKMLEPKYVHPYITGLLAQSYGYKITILKHESSVHIVLTLPDA"
     gene            complement(10019..>10984)
                     /locus_tag="PK513_06175"
     CDS             complement(10019..>10984)
                     /locus_tag="PK513_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOO82069.1"
                     /translation="GEGASNLIDVRENYIARLLQDFTGQRDLKVAWDAGNGAAGEIMR
                     RLCEKLPGEHILLFDEIDGNFPNHHPDPTVDKNLIDLQKAVRDNGCDLGIAFDGDGDR
                     IGVVDENGSVLRCDILMTIYAQEVLETHPGAAIIGDVKCSQVMFDEINALGGQAIMWK
                     TGHSLIKDKMLDMNSPLAGELSGHIFFADKYYGFDDALYCAIRLLNAVDAAQGPLSSL
                     TQNLPKLFNTPEVRVDVDEEHKFGVVPQIIENLKPALPDDHSLDDIDGMRVSSPDGWW
                     LLRPSNTQAVLVFRAESHSEAGLERLKTMAKNEVEKLGFQLNFDH"
BASE COUNT         2856 a         2901 c         2330 g         2897 t
ORIGIN      
        1 gccgcgcctt taccatgctc catctcataa agaactttgg cggcctcttc gaagcttttg
       61 ttctttaaga gatcgtcttc tttcaattct ccagaaaaca gactggtatc aacttcaaaa
      121 gaaagcttgg ccgcgtcttt tttctcttgc cagaagtcaa acagcgcttt atccgcgcca
      181 ttatcaatat cttcctttgt caatgtcagg cgcggatcat tactcgcaaa acgatgccgc
      241 gccgccagcg gcccttcaag aaccgccgga tcaagagcta taagctggcc atcgcttccg
      301 cgcagctcca taaccgtttc cgttgtttcc ggttctgtct gttgagtagc cacagccgcg
      361 cctgctgccc cggtcgtaac ggcaacaggg gtaaccgcgt ctgcagcagg gctttcattg
      421 tcatcagagg taaggacagc aggcctgaaa gaggcagggc gatagtaggc gtagccgccg
      481 ccatatagca tagacataat atccgttatg ctctcttcgt ccttctttct cttttcttct
      541 gccgtatcgg ccatttcaat accctcttaa attactcttt ttattcacgc ccttttttac
      601 ggacttatta ttaaaattct tatgatggct ccgcttccgg cgctctaaaa tcaattccca
      661 aatcaaaggc cccgtttttg ttttgcagga catattcagg gttattccgt gaaggaacat
      721 taggcaaagc cagttcctgc gtatcggccc cggcgacctc aaaatcaccc tgaagttttt
      781 ccgatgaata atgttgtacg atcgtgacaa gaccgataac cacagctgcg gccatagcaa
      841 ggccgcgtaa aggtttgggg agtttcatcg cgccgagcaa gccgcccgcg cctaaaagtg
      901 ttttgcccca gtggtcttta aagaaattgc ccatagcgga atcatcatcc agcccggctt
      961 tatcgagcgc gccatcaaca acctttcctc caaggttcga ggcgctgtcg ctgataaacg
     1021 cgccagccgt ttcgttcagc gcaccatcgc tcaatttgtt ggcgccgtac cagccaccgc
     1081 ctgcgagtgc caagaatggt aaccaaaatc ccatataaaa cctttttcat ctttgcagac
     1141 atctatctat ggtgtctgcg ggttattctt tagtaagggg gaatgaaagg ggatttcgtg
     1201 tattaagcct tgaaaacctg tctttttcat accctcttat tatcttggat tttcaacctc
     1261 ttaaccgggt ttttcatccg tccggacgca cacttaaaac actgcgccct tatatttcag
     1321 ttctcatggt atagatgcag ggttaaaaaa gtcttaacaa aaccgttaag aattataaaa
     1381 atatggaaaa agtttacggg gcgtcatccc cgcagcgacc gcgaagcggt ctggcatagc
     1441 ggggatctgg gaaaagagaa ccgctaaaag cggttcgcat atggatcccg gatgtttaag
     1501 caatgcttcc gcctacgcca agacttcggc ggacaagtcg catcgcctcc gggatgacat
     1561 cataagaagg gatattaatg cctaaagtgc cgcatgccag tcatcaccat ggcaaggccg
     1621 cgttcattcg ccgcttcgat gacttcctca tcgcggatag acccgcccgg ctgaataact
     1681 gccgtaacgc cggcttccgc cgcgcttatc agcccatcgg caaacgggaa aaacgcatcc
     1741 gaagccacaa ccgagccttt ggccagcggt tcatcaaggc ccagctctct ggctgcttcg
     1801 gcggatttcc acgccgcaat gcgtgcgctg tccacccggc tcatctgtcc ggcgccaata
     1861 cccacagtcg cgccaccttt ggcataaaca atcgcattgg acttcacatg cttggccacg
     1921 cggaaagcaa acagcatgtc tttaagttct tctttggtcg gttcgcggtc cgtaaccacc
     1981 ttcaaatcgt cttccaatgt ttgcccgtta tcggaatcct gcaccagcaa gccgcccgca
     2041 atgcgcgtga ccatccggcg tttttgcgtc aaattcggca tcccgcctgt ggtcagcacc
     2101 cgcacattct tcttggcttt caggacttca atcgcatcgg cggcaatatt gggtgcaata
     2161 attacttcac agaatttctg caatatggct tcagccgtgt cttttgttaa agtgcggttt
     2221 agcgcaataa tcccgccaaa ggcgctcacc gggtcgcagg caaaagcgcg cttgtaagcc
     2281 tccagcatat catcagccat cgctacgccg cacgggttcg catgtttgat aatcgccacc
     2341 gccggctcat caaattcggc tacaagctca aacgccgcat ccgtatcgtt gagattgtta
     2401 taagacagct ctttcccctg agtttgcgtt gcgcgcgcag cgccgggacg tacagagcca
     2461 tcaaccgtat aaagcgccgc attttgatgc gggttctcgc catagcgcag cgtttgtttc
     2521 agcgtccccg aaaagctgat ccgtcttgga aagccaaaat catcccccat ctgctcaaca
     2581 aaccaactgg caatattgga atcatatgtt gcggtaagcg catatgctgt cgcagccaaa
     2641 gctttgcgtg tcttatatgt caccgccccg tcatggtgat ccatgtccgt caaaacttta
     2701 tcataatcct gcggatcggt aatcacagcg acaaactcat gattcttggc cgctgcgcgg
     2761 atcatcgccg gaccgccaat atcgatattc tctatacatt catcataatt tttgccgctt
     2821 tgaaccgttt ccatgaacgg atagagatta acgacaacca tatcaatatc gacaatatca
     2881 ttgtcttcca tcgcctgcac atgcgccttg tcactgcgcc gtgctaaaat gccgccatgc
     2941 acactcgggt gcaatgtttt aatgcgccca tccataattt caggaaatcc ggtatgttcg
     3001 gatacatcct tgaccgctat gcctgcatct ttaagcatct tggccgtgcc accagtagaa
     3061 agaatttcca ccccgtgtgc ttcaagtttt cgtgcgaaat cctccatgcc gtttttattg
     3121 gataccgata ttaaagcacg cttgattttg accgcgcgcg gatcgcggat aatattttct
     3181 tggaaatcat ttggggcggc agcttgtgaa acttcggaag aactcatgga cacgtctctt
     3241 atgtttttaa agattgcaga cgtgattatt taagccttta agcaaactta agcaagagct
     3301 tgcgtatcgg tttgtgatat tttcaaaaga ggactgcctt caacggcgta aagaccgcgc
     3361 ctatcgggaa taggtttgaa agagtcggta atgcccaaaa ctgtttccgc cccgcccaaa
     3421 aacaaaaagc cgtctttttc aatttgctgg gccattttgt tcagcacggc gcccttcgtc
     3481 tgctcatcaa aatagatcag cacattacgg cagaaaataa tatcaaacgt accaagtttg
     3541 gtcatagggt ccagcaagtt gaaatagtca tatttgacca ttttacgaat atcgtcttta
     3601 atctgccagc gatcaccgtc ttgtgtaaaa tatttcataa gcagttgaat aggcagaccg
     3661 cgctgcactt caaattgcgt atatctcccc tcacgggcaa ggttgagaat gtcatgcgaa
     3721 atgtctgtag cgataatctc aaaactccag cctggaaatt ccatcgccgc ttcttttaaa
     3781 agcatggcaa tcgtataagg ctcttgcccg cttgatgcag cggcgcacca tatgcgcaga
     3841 cgtctacgcg gagcacgggc ctctttcata tattttaaaa ccgtatcttt aaacagatca
     3901 aagggacggg aatcacgaaa gaacgaggtc tcattggttg tcatcgcctc tacaatatca
     3961 cgaaccagct ctttatctgg tacgccctgc agggtcatcg tcatcgcctc aagtgaatcg
     4021 taaccccatt ttttagccac agggttaaga cgagattcca ccaaatagga tttatcctga
     4081 cttaaaacaa gtccggattt ttccttgagc aaatctttat aaatatcgaa gtctgtaatc
     4141 ttcataatgc tatcctgcta tggtctttct aacatatggc ccaatctcgc taagtggcaa
     4201 cacattcgta caatacccgg ctttggcaac ggcgcctggc attccccaaa caatactgga
     4261 cgcctcgtct tgcgcaacca gacgcgcccc gcaatcggta agaagcttac ccgcaccaat
     4321 accatcattg cccattccgg tgagaataat gccgtaaacc ttattgccaa aaatgtcgat
     4381 aacactacga aacatcggct caacagatgg tttgcagaaa ttctcaggtg ggccattatc
     4441 cagtctggca accaacccct cagcttcctt ggaaattttc atgtgaaaac ctcccggagc
     4501 aacatacgct ttgccgtttt caatacgcat gccatccgaa gcttcatgcg ccttgcaacc
     4561 cgtatgttgt gtgatgtgct gggccaggat ttttgtgaaa gttgcgggca tgtgctgcgt
     4621 aatgacaata ggaatacgaa tgccagcaaa atttttgatg acttcaaaca atgcttgagg
     4681 ccccccggtt gaagaaccaa tggcgataat agaagggctg ccgggataaa caccaacgct
     4741 tgatcgcaaa gaaaattctt taagcgttgt ttgcgccgga ggggtgctcg ccgaagggtt
     4801 cgttacatct acagcacggc gctcactttc agggacaagg ccgcggataa ttttcaacaa
     4861 attgcgctga aactctgaac cttcaccaac gccttgtgtg cttgatggtt tgacaataca
     4921 ttcgacagcg cccagagaaa atgctttaag cgtcacgtct gcgccatttt ccgtcagaga
     4981 agagaacata atgacttttg aatttgggct tgcttccaga attttgggaa gggctgtaat
     5041 accgtccatc accggcattt caatatccag aatgacaata tccggcttat atttttctgc
     5101 agagcggatc cccatctcgc cattcgcaat cgaggccaca acggtcatat ccggcgcagg
     5161 atctataatg cgccccaaag caccgcgaat aaccgcagaa tcatcaacca gcatgatatg
     5221 gattttttta tttgaagtcg tactcataag ttgaagccaa taatccttct attgaatctt
     5281 atatgttagc tggattgttc aagtaatccg atttgtatga atttagattc gataatttca
     5341 ctatcaaagg gcttcataat atattcattg gctcccgcct gaatcgcgcg ctggatatgg
     5401 ttcatgtcat tttcagttgt acaaaaaaca accttgggat ggctgccatt ctccatttgg
     5461 cgtaattttt ctaaaaactc aataccgctc atgatgggca tattccagtc cagcaatacg
     5521 gcatcaggca ttttgacctg acaggcatca taggcttttt gaccatcctc agcttcatca
     5581 cattcaaagc ccagatcttc ggctatgcgc cgcgcaacct ttcgtacaac gcggctatca
     5641 tcaacaataa ggcaagattt catgaatagg gccctttcag attttaatca ttatgagcag
     5701 tttacactta aaaaacattt aatatagagg cgagttaaaa agattgatca agaatgaaca
     5761 ctgctgacaa aatttggaac atccaaaatg accaaaagtt catcgtcaag acgataaaca
     5821 ccggatgaaa tttcacgcca aagtgaatcc agtgtcgagg gagttgattc aaaactttca
     5881 ttacgcaaac gcaatacatc gcccacttca tcaataatca ggctgtaaag ctcattttca
     5941 tgttcgacaa caacccccat agtcgatgag gtggttttac tgcacggttc aatgcccaac
     6001 cggcagcgca tgtcgatagc cgtaacaatc cgtccgcgta aattcaaaga ccctgcaact
     6061 tccggcggag ccagtgggat ttttgtaacg gtctgctcac ccagaacatc ttgaacttgc
     6121 aagataggga ttccgaaacg ctgaccagct ataacaatag tcagaaattc ttttgactgc
     6181 gtgtcatgct ggttttctga accctcataa tccaaggcat ctgcatttgc gctcatgcca
     6241 catctccttg ttttgtcaga tcatagtttt gttgctgttc agctatcgtt tgtgacaacg
     6301 tattgagcag tgtatcgcgg tcaaattttg cgatatattt cgaaaagcca acgctcatgc
     6361 cccggtcaat atcctgaggc gtggcgtgtg agctgagggc cagcatcggt gtttctttcc
     6421 agttggtttc gcttttcacc ttctgcgcaa attcaaaccc gtccatatcg ggcatttcga
     6481 tgtcactgat gatcacatca aattccgcgc ctttatcaca catttttaag gcatcatgag
     6541 gactttccaa agaaattaca tcatacccgg cgacactgag caggggtgtg agcatattac
     6601 ggaaaaatgg gctatcatca accagcaata cccgtttttt agcgtggcca ttgtttttgc
     6661 cgttggtcgt atctttcgaa gagccgtaag gtgtgtcatc atggtcttta aaccaatccg
     6721 aaccattggc ctgaaggaaa tgcccgacat caacgatgtc cgtggcttta ccactgataa
     6781 tggcgctgcc catcatccca ccttgcccgc cttcaagctg taggttgata tgttcttcgg
     6841 taatatcgat aatttcatca acaataatgc ccaaagaacg tcccttatcg gcaaagacca
     6901 gtacaggttt ctcccccgtt tcccctattt gcactcgtgg atcaagcggc ataagcggca
     6961 taagtttacc gcgatactgc accatcatct gcccggcaga acgctcaatg ttgctgacat
     7021 caatattttc aaggcgcgcg accaaggaaa gaggaacggc ctttggcgtt ttgttgcctg
     7081 cggtaaacaa caacagagaa gttttctctc cgcgcgcaga atcaatggct gcatcctgat
     7141 caagcgtgcg tgcgccgtct gtagcgtcaa cctgcccgat atgtttggca ataccgttcg
     7201 ggtctaaaat catgataacg ctgccatcac ccaaaatagt atttcctgaa aacatctcaa
     7261 tattgcgcaa aattttggcc acgggcttga caacaatttc ctccgtatca aacacgcgat
     7321 caacgataat gccaaaatcg ttattcccaa cctgagtaac aacgatatat tggtgacgct
     7381 ttttcggatt ggaaaccgaa tcaatcttag gcgaactatc ctctggcggc gtatcatcac
     7441 tttcagcaga attgctctct tgagatgcat cggcaacaac ctttttatct ggcggtgaaa
     7501 gtttgagaag atcggaaagg gagataagcg gcaacagacg atcacgcaac cggaagacag
     7561 gcgtaccttt tatagtttca atacggtttt cgccgtcttc ggaggccatc accagctcac
     7621 gcaccgccaa ctgcggaatc gcaaaacgct cctgcgcaga ctcaacaatt agcgccgaaa
     7681 caattgcaag ggtcagcgga atcttaattg taaaggtgga gccttttcct tccatggact
     7741 gtaagtcaat tgagccgccg attttctcaa tattggtgcg cacaacatcc atgccaacgc
     7801 cgcgccctga tacggctgta actttttcag ccgtagaaaa accggcgttg aaaataaact
     7861 gattgatctg attggaggac atttcctcaa gctcttctgc tgttgccagc ccattttcca
     7921 agactttggc cttgatttta tcgagcggca accctttacc atcatcagaa atttggatga
     7981 taatatgccc gccttcatga aaagcattca gtagaatttt tccggtttcc ggtttcccgg
     8041 ccgccttgcg cgtttccgga tcttcaatgc cgtgatcacc ggaattgcgc accatgtgag
     8101 tcaagggatc tttgataagc tcaagaacct gacggtcaag ctcagtatcc tgacctttca
     8161 tttccagctc gattttctta ttcacctcaa tcgccagatc gcgaataata cgcggcaact
     8221 ttgaccatgc gttgccaatc ggctgcatgc gtgtttgcat tacgccttcc tgcaattcgg
     8281 ataccacatg gttgagtcgt tgcaacggca cagaaaattc actgtcggaa ttctggcgca
     8341 agatctggat gagctggtta cgcgtcagca ccatttcact aaccatcgtc ataagattct
     8401 caagcacgtc gacgcttacg cgcaaagact gcgcagaaac aggcttttca gccgtttttg
     8461 ctacggaagc gtcattagcc ggagcggcag cttttggcgg ttcttccttg ataacagttg
     8521 cttcttccgg tgcgctgggc gcatcagcgc cgggctcttc accaacggtt ttaagcgcaa
     8581 attcctccgg tccctcagca ttttggaagg cagcctccag cgcatcaagt tcttcctgag
     8641 taagacaatt tccaccttcc aaaggtgttt caacagcttc ttttggggag acttcagcgc
     8701 cggaggaagc tgcgccgccg ccgtcacgtc cctcataaac ggcatcaagc ttttcaatca
     8761 acgcgctgtc atcaccttcc ggctctgaac cggtctcctc taatcctccg acgatgagct
     8821 taatgcggtc aatcgattca agaatcagcg tgacgtaatc gggcgtaact tccagatcgc
     8881 catcgcgaaa acgccccaaa acattttccg cgtggtgtgc aactttctcc aaccgcggca
     8941 aacccaaaaa accgcacgtc cctttaatgg tgtgcatcaa tcgaaaaatt ttagacaaca
     9001 gatctttatc attaggattt tgctcaagat taaccaggtc caggtcaagc tcatccaaac
     9061 tctcattagt ctcggtcaag aattcattga ttagatcatc catagtatca ttcctcctac
     9121 atcatcttct atgacgtggt tttcgcatat aaaattcggg tgatcttaca ccttaacaca
     9181 aagacgttag ccaaggctta aaacgccttt ctatttgtaa aatcacgaaa tacgcagcta
     9241 aacacagcgg atacgcagaa atgaagtaca gattaagtct atacgatctc tttgcaattc
     9301 agaatagcag aaccgaaaaa atactaaaaa tctttattta tgtgtgcagt ggtatgagta
     9361 tcaagcgtca ggaagggtaa ggacaatatg tacactactc tcatgtttta aaatcgtaat
     9421 cttatagccg tagctttgtg ccagcagtcc agtgatgtaa gggtgtacat atttcggctc
     9481 cagcatcttg cggctcatag aaaggtcaag agcctgcgca gtttgttcgc gaatgcccgc
     9541 atcttgtccg ctgccactta ccaatgtcga tccgttattt tcaccggcct caaccttaag
     9601 tattcctcct ttaggcaggc aatccatcac cagcaacaac gcgcaaataa gcatcttgca
     9661 aaaagctttc ggacgatcct cataccccaa tggggcatac gggtcccatt cctgaacaat
     9721 ctttttttct gccccgacaa tatcctcaat cgcccggtgc acctcttcgg gcttgatgga
     9781 atcatctgcg ccgcctgcgc cataggccat ccggtaggcc ttaagcttgg ctgatgcctg
     9841 agacgcgcta aaagcaatta aatccgtaac ctcttcaccc gcatccggcc ccatttcggt
     9901 taaaaactca ataccattat ttacagcgcc aatagggctg atcaaatcat ggcagatttt
     9961 tgaagccata agctccaaaa tatcagtatc aattgtaacg ctcatggaaa tttatccttt
    10021 aatgatcaaa attgagttga aaaccaagtt tctcgacttc gttttttgcc atggttttta
    10081 gtcgctccag ccctgcctcg ctatggcttt cggcgcggaa caccaaaaca gcctgcgtat
    10141 tggatggacg caaaagccac cagccgtcag gcgaagaaac gcgcatgccg tcaatatcat
    10201 ccaggctgtg atcatcgggc agggcaggtt ttaagttttc aatgatctgc ggcacaacgc
    10261 caaatttatg ctcctcatcc acatcaacac gcacttccgg cgtattaaaa agctttggaa
    10321 ggttctgcgt aagtgaagaa agcggtccct gcgctgcatc aacagcattc aacagacgga
    10381 tcgcacaata aagcgcatca tcaaagccgt aatatttatc ggcaaaaaag atatggcccg
    10441 aaagctcgcc ggccaaaggc gaattcatat ccaacatttt atccttaatc agagaatgcc
    10501 cagtcttcca cattatagcc tggccaccca gagcgttgat ttcatcgaac ataacttgcg
    10561 aacacttcac atcgccaatg atggccgccc ccggatgcgt ttccagaacc tcttgtgcgt
    10621 aaatcgtcat caaaatatca cagcgcaaaa cgctgccatt ttcgtcaact acgccgatcc
    10681 ggtcgccatc cccgtcaaac gcaatgccca gatcgcagcc attatctctc acggcttttt
    10741 gaaggtcgat caggttttta tcaacagtgg gatcagggtg atgattaggg aaattcccgt
    10801 caatttcatc aaacagcaaa atatgttcgc ccggcagctt ttcgcataaa cggcgcataa
    10861 tttcgcctgc cgcgccattc ccggcatccc acgcaacttt caaatcacgt tgtcccgtaa
    10921 aatcttgtaa aagtcgcgcg atataatttt cgcgcacatc gatcaggttg gatgcacctt
    10981 cacc
//
LOCUS       DAOSWR010000067        10866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3829_length_10866_cov_5.440107, whole genome shotgun sequence.
ACCESSION   DAOSWR010000067 DAOSWR010000000
VERSION     DAOSWR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_10866_cov_5.440107"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /gene="dnaE"
                     /locus_tag="PK513_06180"
     CDS             complement(<1..2532)
                     /gene="dnaE"
                     /locus_tag="PK513_06180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOO82070.1"
                     /translation="MNHFIHLHVHSAYSLAEGAIKVKGLVNRCVAENMPAVAVTDSSN
                     MFGAMEFATEATKNGVQPILGAQVLYGDAEHQLVLLVQNEQGYKNLCRLLSDAYMGGD
                     AASKAVISKEELGAFSDGLLCLSGGSKGPVNDALFHKQAEKAEEEFIYLKECFDGRLY
                     SEIQRHGWAAEDATEEALIELSYKYDVPLVATNDCYFMDREAHDAHDALLCISEGRYV
                     TEEDRRKVTPEHYFKSADEMCRLFADLPEAVANTAVIAQRCSFLLEPIAPILPAFDTE
                     GGRSEFEELKAQSEAGLEWRLKNFAKGVDAKPYFERLEFELDIINKMGFPGYFLIVSD
                     FIKWAKEQDIPVGPGRGSGAGSVVAWALKITDLDPLELDLLFERFLNPERVSMPDFDI
                     DFCQDRREEVIHYVQQKYGRDRVAQIITFGKLQARAVVRDVGRVLQMPYGQVDRLAKL
                     IPNNPANPVTLQEALDDDPDLRLEIKKDETSVRLIEIALQLEGLYRHASTHAAGVVIG
                     DRPLHELVPLYRDPRSDMPVTQFNMKYVEQAGLVKFDFLGLKTMTVVHKTLKMVEEQT
                     GERIDTLEIPLDDKSTYEMMTRGATVGVFQLESAGMREVLRKMHPDRFGDIIALVSLY
                     RPGPMDNIPTYISNKEGKTEPDYMHEKLQPFLEETYGIMVYQEQVQRAAQALAGYSLG
                     GADLLRRAMGKKIKAEMDAQREMFVKGAKEHNNVPAVQANQIFDQIAAFAGYGFNKSH
                     AAAYALIGYWTAWLKCHYPLQFMAASMTLDLGNTDKLSIFKQDLDRMGVELRQPDINE
                     SNADFKVEGEGVRYALGALKGVGEAAMRTLVAERAEHGPYASIEDY"
     gene            complement(2566..3264)
                     /locus_tag="PK513_06185"
     CDS             complement(2566..3264)
                     /locus_tag="PK513_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO82071.1"
                     /translation="MNNPLDQDVLLKLEGLEKTFEQGGRKLTIFRGLDMKIEAGELVA
                     LVGQSGTGKSTLLQIAGLLDKPTAGRVVINGRDCVKLGEQKRTAMRLSQIGFIYQFHH
                     LLPEFTALENVAMPQIIAGARVSNAKDKAQGLLRSLGLGKRLEHRPSTLSGGEQQRVA
                     IARALANDPKLLLADEPTGNLDPETSGEVFDILLQQVREREIGALVATHNLQLADQMD
                     RALELKAGRIVPFD"
     gene            complement(3274..3696)
                     /locus_tag="PK513_06190"
     CDS             complement(3274..3696)
                     /locus_tag="PK513_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO82072.1"
                     /translation="MHWLVYALVVIAGGLGIHSFSKLAKSYIDPVYTFLLAGGVFFTI
                     SILTFLIMGGREHIAQTPTKGIILALCTGVAMTAANFGVFLMYKAGAPMSVAMPLTRT
                     STAILAVLFGVFFFAEKLTLVNMTGVVFAVIGIVLITR"
     gene            complement(3697..4938)
                     /locus_tag="PK513_06195"
     CDS             complement(3697..4938)
                     /locus_tag="PK513_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOO82073.1"
                     /translation="MLSTFERMVAWRYLRSKKAEGFVSVIAGFSFAGIMLGVATLIIV
                     MSVMNGFRAELFDRILGLNGHMNIYSQSGPLYDYDYIQSRVEPISGIKMVVPIIESQA
                     LVSKGGSSSGVLVRGMAWKDFANREILRDSITQGKLEDFTGNGIALGEALAQKMRLRP
                     GDKITLTSPQVKSTPFGSMPRQRSYTVALIYDVGMYEYNSGFIFMPLEAAQKFFQFDG
                     AVTALEVFLKDPDELSVVRKAVSMVIEGQAGIYDWRDMNASFFNALQVERNVMFLILT
                     LIILVAAFNIISSMIMLVKDKGQDIAILRTMGASRASMMRIFMLTGASIGIVGTLVGA
                     ALGIAFALNIESIRQFLEGLTGTELFAEEIYFLSQLPAEIEWHEVAAVVGMAFFLSVL
                     ATLYPAWRAARMDPVEALRYE"
     gene            complement(4940..5146)
                     /locus_tag="PK513_06200"
     CDS             complement(4940..5146)
                     /locus_tag="PK513_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82074.1"
                     /translation="MDLTFILYAVFLLAVVVLAIYHRNKDQKKVMNYYEHASEKGDEV
                     AKKSLENQKRMIELLETISKKLDK"
     gene            complement(5167..6624)
                     /gene="proS"
                     /locus_tag="PK513_06205"
     CDS             complement(5167..6624)
                     /gene="proS"
                     /locus_tag="PK513_06205"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO82075.1"
                     /translation="MSQKAKTAITPTREENFPEWYQQVIKAADLAESSPVRGCMTVKP
                     YGWAIWENMVGIFDPWLKEYGVQNCSFPMLIPVSFLSKEAEHVEGFAKECAVVTHHRL
                     EADPDGNGLRPAPTAELEEPYVVRPTSETIIGDAMARWVQSYRDLPLKLNQWCSVMRW
                     EMRTRIFLRTSEFFWHEGHNAFADHEGAKADCLHVLDLYEKFFTDVLAIDGFKGIKTE
                     EERFPGANETYSFEVMMQDGKALQASTTHDLGTNFAKTFDIKYQTKEGGEELCHTTSW
                     AFTTRMIGALIMMHGDDDGMIMPPRIAPQQVVILPVLKGDNDEAIMAYCSEIAAQLKE
                     KGIKAKVDARDMRTPDKMWDAIKKGIPLRVEVGGREMEEAQVTHVRRDLGRESKTTQS
                     VESFVEDARGLLDAIHDDMLARSRSFRDENTHEGQTLADIEAFFKAGKTGLVKVPVAV
                     LEDVGLDAVMKEYGLTTRCMPFVDNGRTVLIAKAY"
     gene            complement(6641..7123)
                     /locus_tag="PK513_06210"
     CDS             complement(6641..7123)
                     /locus_tag="PK513_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82076.1"
                     /translation="MRWFTGLLVFFVPVAALAQDASLAVICKALPNYKTPAGVEYVQG
                     VEGVVPADLNPLKAPMLDVVKIPIDVMLAERFQSVKIPSDLELQPNVAMISIYRDGRV
                     EYNGQDVSGQAYSLCGKSVGIVEKSRNGQAGDDALKSQPEVIELKESVEGEVLEGQYP
                     "
     gene            complement(7120..7368)
                     /locus_tag="PK513_06215"
     CDS             complement(7120..7368)
                     /locus_tag="PK513_06215"
                     /inference="COORDINATES: protein motif:HMM:NF018978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HOO82077.1"
                     /translation="MGLVTGIIVFLLIWWVSLFAVLPFGHAREVDGTPVKANLKRKFI
                     WTTLVAMVVWGIVFVLIEAEIVSFREIANEMALEDKLR"
     gene            complement(7373..9055)
                     /locus_tag="PK513_06220"
     CDS             complement(7373..9055)
                     /locus_tag="PK513_06220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOO82078.1"
                     /translation="MSVARDLMSQDGLFFIPLGGSEQFGVNLNVYVSDGQMLAVDCGI
                     GFADDRFPGIDLLLPDPTFLEERREALKGLIITHAHEDHVGAVAYVWDRLQCPIYASP
                     FTAAVLRKKLEEQNVRRVKIEIITPGVQVQIGAFGVHFVPVSHSVPDTCSLIIDTDHG
                     KLVHSGDWNLDPAPVTGAATDAAIFKRAGEEGVLAYIGDSTNAEVDGRSGSESVVAKG
                     LEAEFRKCDGKIAVTTFSSNIGRVISILKAAKACGRQVGVIGRSLHRMIGCAADCGLL
                     DKNLPDFVSEEDLGYLPDDKVVVIVTGSQGEYRAALAKVARGVFKGFKLNKGDSVIFS
                     ARAIPGNEVSINQVKNNLSAAGVNIITPRDTKNIIHVSGHPCRDEIADMLRWVRPQVV
                     VPVHGERVQLDAQAAFARDCQVPKALVPSNGSVIRLAPGNPEIVDHIETDILAVDQKR
                     IVKATHQSIAARRKLQYTGAVHISLAVDARGKFLGDPKFEMLGLLDLDNPGEEQIEDK
                     LYDEILDLLEELTFEERLDDHFIAEELRIGIRRFCDHTLGIKPKTTVHVLRV"
     gene            complement(9052..9831)
                     /locus_tag="PK513_06225"
     CDS             complement(9052..9831)
                     /locus_tag="PK513_06225"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOO82079.1"
                     /translation="MLLAIDVGNTNIVFAVFDGGIIRGAWRLHTYARRCADEYAVFLH
                     QILVMDGLSFINIHDVVISSVVPETHFHLGCFCEKHLHLVPVFITKDHVPITIDMERP
                     EDVGADRLVNALAVNMHYGERIGTGGVIVIDFGTATTFDVINASGAYAGGAIAPGIDL
                     SVNALHSAASKLPKIGVRKPAQAIGKSTSEAMRSGVYWGYVGLIERITGQISAELGGE
                     KPFVLATGGLAALFEDAGVIDLIDQDLTLKGLLAIYQEMHG"
     gene            complement(9815..10495)
                     /locus_tag="PK513_06230"
     CDS             complement(9815..10495)
                     /locus_tag="PK513_06230"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO82080.1"
                     /translation="MHWDIQVFTKLNSTQDECKRLAVDGVPEGAVVQAIQQVLGRGRH
                     GRIWLSEEGNLAMSFILRPRCEIVKIGQISIMIGVALAQTIGKKAQLKWPNDVLVDGR
                     KCAGILIDSDLSGGALNWLVIGIGVNVASAPEIGAVLNVDGDKFRDALLANIAQLYMR
                     WQRDGFADIRLEWLARTYEKGMALNVGAFESLDELGNLIVRDRQNQLQTISAGDVFLK
                     DRDYAAGD"
     gene            complement(10501..>10866)
                     /locus_tag="PK513_06235"
     CDS             complement(10501..>10866)
                     /locus_tag="PK513_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO82081.1"
                     /translation="GLSRHAPGLAYAMAILMFSMSGIPPAAGFFGKLVVFNAAVAEGY
                     YILATLGVLTSVVAAYYYLRVIKVMFFDEPADVFDGPIPFARRVVLFVCILFVLGFVI
                     KPSVFIASSFNAASVLFAG"
BASE COUNT         3023 a         3444 c         2019 g         2380 t
ORIGIN      
        1 ataatcttca atcgacgcat acggcccatg ttcagcgcgc tccgccacca gcgtacgcat
       61 cgccgcctca ccaacgcctt tcaaagcgcc aagcgcatag cgcacacctt cgccctcaac
      121 cttaaaatcc gcattcgatt cattgatatc cggctggcgc agctcaaccc ccatccggtc
      181 cagatcctgc ttaaaaatac tgagcttatc ggtattcccc agatcaagcg tcatcgaagc
      241 cgccataaat tgcagcggat aatggcattt cagccacgcc gtccaatacc caatcaacgc
      301 ataagcggcc gcatgtgact tgttaaaccc gtaaccagca aaagcagcaa tttgatcgaa
      361 aatttgatta gcctgcaccg ccggaacatt attatgctct ttcgcgcctt tcacgaacat
      421 ttcgcgctgc gcatccatct ccgccttaat cttctttccc attgcccggc gcaaaaggtc
      481 ggcaccgccc agagaatacc cggccaaagc ctgagccgcc cgctgcacct gctcctgata
      541 aaccataatt ccgtacgtct cttcgagaaa aggttgaagc ttttcatgca tatagtccgg
      601 ctcggtctta ccttccttgt tactgatata ggtcggaata ttatccatag gcccgggacg
      661 ataaagcgaa accagcgcaa taatgtcacc aaaccggtcg ggatgcatct tgcgcaaaac
      721 ctcgcgcatt ccggcacttt ccaactgaaa cacaccgaca gttgcacctc gggtcatcat
      781 ctcataggtc gatttgtcat caagcggaat ttcaagcgta tcaatccttt cccccgtctg
      841 ctcttccacc attttgagtg tcttgtgcac caccgtcatg gttttcagcc ccaagaagtc
      901 gaacttgacc aaaccagcct gctcgacata tttcatgttg aactgcgtca ccggcatatc
      961 agaacgcgga tcacgataaa gaggcaccaa ctcatgcaac ggccgatcgc caatcaccac
     1021 cccggccgca tgcgttgaag cgtgccgata taacccttca agctgcaaag caatctcgat
     1081 cagacgaact gaagtttcat cctttttgat ttcaagacgc aaatccgggt catcatcaag
     1141 agcctcttgc aacgtcaccg ggtttgccgg gttattagga ataagcttgg caagcctgtc
     1201 cacctgtcca tagggcatct gcaacacgcg ccccacatcg cgcaccaccg cgcgcgcctg
     1261 caatttcccg aaggtaatga tctgcgccac ccggtcccgg ccatatttct gctgaacata
     1321 gtgaatcacc tcctcgcggc gatcctgaca aaagtcgata tcaaaatcgg gcatagaaac
     1381 gcgttcgggg ttcaaaaacc gctcaaacag caagtccaac tccagcgggt caaggtcggt
     1441 aattttcagc gcccaggcca ccaccgagcc cgcacccgaa ccccgtcccg gcccgacggg
     1501 aatatcctgc tccttcgccc acttgataaa gtccgaaaca atcaggaaat agcccggaaa
     1561 acccatttta ttaataatat ccagttcaaa ctccagacgt tcaaaatacg gcttcgcatc
     1621 cacgcccttg gcgaaatttt taagacgcca ttcaagtcca gcttcggatt gcgctttaag
     1681 ctcctcaaac tcgctgcgcc cgccttccgt atcaaacgcc ggcaaaatcg gcgcaattgg
     1741 ttccagcaag aacgaacaac gctgcgcaat cacggcggta ttagcgacag cctccggcaa
     1801 atccgcaaac agcctgcaca tttcatcggc agacttaaaa taatgctccg gcgtaacctt
     1861 gcgccgatct tcttcggtca catagcgtcc ttcagaaatg cacaaaagcg cgtcatgcgc
     1921 gtcatgcgcc tcccggtcca taaaataaca atcattagtc gccaccaacg gcacatcata
     1981 cttgtacgac agctcaatca gcgcctcttc cgttgcgtct tctgcggccc acccatgcct
     2041 ttgtatttca ctataaagac gcccatcaaa gcattctttt aaataaataa attcctcttc
     2101 cgctttttct gcctgcttat gaaacagcgc gtcattcacc ggcccttttg acccgcctga
     2161 aagacacagc aacccgtctg aaaatgcccc aagctcctcc ttcgaaatca ccgccttgct
     2221 cgctgcatcc ccgcccatat aggcatctga aagcaaccgg cacagatttt tataaccctg
     2281 ctcattttgt accagcaaaa ccagttggtg ctccgcatca ccataaagaa cctgcgcccc
     2341 caaaatcggc tgcacaccgt ttttagtcgc ctcagtcgcg aattccatcg ctccgaacat
     2401 attggaagaa tctgtcaccg ccaccgccgg catattctca gccacgcagc gattcacaag
     2461 gcctttaacc ttgatagccc cctcggcgag cgaatacgcc gaatgcacat gcagatgaat
     2521 gaagtgattc atggaacgag tttagcagga cacagaaaca aaatactagt cgaagggaac
     2581 aattcttcca gcctttagtt ctaacgcgcg gtccatttga tcggcaagtt gcaaattatg
     2641 cgtcgccacc aacgcgccaa tttcgcgttc ccgcacctgc tgaagcaaaa tatcgaagac
     2701 ctcgcctgaa gtttcagggt ccaaattccc cgtcggttcg tccgccagca gcaatttcgg
     2761 atcattcgcc aacgcccgcg caatcgccac gcgctgctgc tccccgcccg aaagcgtcga
     2821 aggacgatgt tctaaacgct ttccaagccc caaagaacga agcaaccctt gcgccttatc
     2881 cttggcgttg ctgacccgcg cgcccgcaat aatctgcggc atcgccacat tctccagcgc
     2941 cgtaaattcc ggcaaaagat gatgaaactg gtaaataaac ccgatctgcg acaggcgcat
     3001 ggccgtacgt ttttgctccc cgagcttgac gcaatcgcgg ccattgatca ccacccgccc
     3061 cgccgtaggc ttgtccaaca acccggcgat ttgcagcagc gtcgatttcc cggtcccaga
     3121 ctgcccgact aaagccacca actcgccagc ctcaatcttc atatccagcc cacgaaaaat
     3181 cgttagcttg cgcccaccct gctcaaaggt cttttccaac ccttccagct tcaacaacac
     3241 atcctgatcc agtggattat tcatgctcat ttcctagcgc gtaattaaaa caatcccgat
     3301 gacagcgaaa acaacaccag tcatattcac aagcgttaat ttctcagcaa agaaaaacac
     3361 accaaacaag accgccaaaa tggctgtaga tgtacgcgtt aagggcatag caacactcat
     3421 cggcgcgcca gctttataca tcaaaaacac cccaaaatta gcagctgtca tagccactcc
     3481 cgtacacaaa gctaaaataa tgcctttagt tggtgtctgc gcaatatgct cacgccctcc
     3541 cataatcaga aaggtaagaa tagaaatggt aaaaaacacg ccgcccgcca aaaggaatgt
     3601 ataaaccgga tcaatatagg acttggccag ctttgagaaa ctgtgaattc ccaaaccgcc
     3661 cgcaataacc acaagtgcgt aaactaacca atgcatctat tcatacctca aagcctccac
     3721 cggatccatg cgcgccgcgc gccatgcagg atacaacgtc gccaacacag acaagaagaa
     3781 agccatgccg acaaccgccg caacctcatg ccattcaatt tccgccggaa gttgcgagag
     3841 aaaataaatc tcctctgcaa acagctccgt tcccgtcagt ccttccaaaa actgacgaat
     3901 actctcgata ttcagcgcaa acgcgatgcc taaggccgcg cccaccaacg tcccaacaat
     3961 gcctatgctc gcgcctgtaa gcataaatat ccgcatcata gaggcgcgcg aggcccccat
     4021 cgtgcgcaaa atcgcaatat cctgcccctt atctttgacc agcataatca tcgaagaaat
     4081 aatattgaac gccgccacca ggataatcag cgtcaaaatc aggaacatca cattgcgctc
     4141 cacctgcagc gcattgaaaa aactggcatt catatcacgc caatcataaa tcccggcctg
     4201 cccctcaatc accatcgaga ccgccttgcg caccaccgac aattcatccg ggtctttcaa
     4261 aaacacctcc aaggccgtaa ccgctccgtc aaattgaaaa aatttctgcg cagcttccaa
     4321 aggcataaag ataaaaccgc tattatactc atacatcccg acatcataga tcagcgccac
     4381 cgtatagctg cgctggcgcg gcatagagcc aaacggcgtt gatttaacct gtggcgaagt
     4441 caacgtgatc ttatcgccgg gccgcagacg cattttttgc gccaaagcct cgcccagcgc
     4501 aatcccgtta ccggtgaaat cctcaagttt tccttgcgta atggaatcgc gcaaaatttc
     4561 ccggttcgca aaatccttcc acgccattcc gcgcaccaat acgccgctgg aactgccccc
     4621 cttggacaca agcgcctgac tttcaatgat cggcaccacc atcttaatac cggaaatcgg
     4681 ctcaacccgg ctctgaatat aatcgtaatc ataaagcggg ccggactggg aatagatgtt
     4741 catatgcccg ttcagcccca aaatccggtc aaacagctcc gcacggaaac cattcatcac
     4801 gctcatcacg ataatcagcg tcgccacgcc cagcataatt ccggcaaagg aaaacccggc
     4861 gatcacgctg acaaagcctt cggctttttt agagcgcaaa taccgccacg ccaccatccg
     4921 ttcaaatgtt gaaagcattt tacttatcca gtttcttgga aatcgtttcc aaaagctcaa
     4981 tcatacgttt ttgattctct aaggattttt tagcaacctc atcccctttt tcggaagcat
     5041 gttcataata attcatgact tttttttgat ctttatttct atgataaata gcgagcacaa
     5101 caacagccaa aagaaaaacg gcatatagaa taaaagttaa atccattatt ttttccttga
     5161 tttagattaa tacgccttcg caatcaaaac cgtacgccca ttatcaacaa acggcatgca
     5221 gcgcgtcgtc aggccatatt ctttcattac cgcgtccagc ccaacatctt caagcacggc
     5281 caccggcacc ttcacaagcc cggttttacc cgccttaaag aacgcctcaa tatcagcaag
     5341 cgtttgccct tcatgcgtgt tttcatccct aaaactgcgc gagcgcgcca acatatcatc
     5401 atggattgca tccaaaagcc cccgtgcatc ttcaacaaaa ctctccacac tttgcgtggt
     5461 tttagactca cggcccagat cacgccgtac atgcgtcact tgcgcctctt ccatctcacg
     5521 accaccaacc tcaacgcgca gcggaatgcc tttcttaatc gcatcccaca tcttatctgg
     5581 tgtgcgcata tcacgcgcgt caaccttggc cttaatacct ttttccttca actgcgcggc
     5641 aatctcagag caatacgcca tgatcgcctc atcattgtca ccttttaaca ccggcaaaat
     5701 caccacctgc tgcggcgcaa tgcgcggcgg cataatcatc ccgtcatcat caccatgcat
     5761 catgatgagc gcaccgatca tccgtgtcgt aaacgcccag cttgtcgtat gacacagctc
     5821 ttcaccaccc tctttagtct gatatttaat atcaaaggtc ttggcaaaat ttgtgcccag
     5881 atcatgcgtt gtcgaagcct gcaaggcctt tccatcttgc atcatcactt caaaggaata
     5941 agtttcatta gcgcctggaa aacgttcttc ctcagttttg atacccttaa acccgtcaat
     6001 agccagcacg tcggtaaaga acttttcgta caaatccagc acatgcaaac aatcggcctt
     6061 ggcaccttca tgatcagcaa acgcattatg cccctcatgc cagaagaact cagacgtacg
     6121 caggaaaatt ctcgtacgca tttcccagcg catcacgcta caccactggt tcagcttcaa
     6181 cggcaaatca cgatatgact gcacccagcg cgccattgca tcgccgataa tcgtctcaga
     6241 cgtcggacgc accacataag gctcttccaa ctctgccgtt ggcgcaggcc gcagcccatt
     6301 accatccggg tcagcctcca gccggtgatg cgtcaccacc gcgcactcct tggcaaaccc
     6361 ctccacatgc tcggcctctt tcgataaaaa actcaccgga atcagcatcg gaaaagagca
     6421 attctgcacc ccgtattcct tcagccacgg atcaaaaatc ccgaccatat tttcccaaat
     6481 cgcccagcca tagggcttca cagtcataca tccacgcaca ggcgaactct ccgccaaatc
     6541 cgcagcttta atcacctgtt gataccattc aggaaaattc tcttcgcgcg tcggggtaat
     6601 ggcggttttg gctttttgac tcattaagac ctctatgact ttacggatac tgaccttcga
     6661 gaacctcgcc ctcgacgctc tctttcagtt caataacttc gggctgagac tttaaggcat
     6721 catcgcctgc ctgtccattc ctggattttt caacaatccc cacagatttt ccgcacaaac
     6781 tatacgcctg gcccgaaaca tcctgcccgt tatactccac gcgcccatca cgatagatag
     6841 aaatcatcgc aacattaggc tgaagctcca gatcactcgg aattttcaca gattgaaacc
     6901 gctcagccag catgacatca atcggaattt tcaccacatc caacattggc gctttcagcg
     6961 gattaagatc agccggaaca acgccttcta cgccctgcac atactcaacc cccgccggag
     7021 ttttataatt tggcaaggcc ttgcatatca ctgcaagcga agcatcctga gccaaagcag
     7081 ccaccggcac aaaaaacacc aacaaacctg taaaccatct catcgtagct tatcctctaa
     7141 agccatctca ttcgctattt cacgaaaact tacaatttct gcttcaatca aaacaaaaac
     7201 aattccccaa accaccatag ccacgagcgt tgtccaaata aatttgcgct ttaaattcgc
     7261 cttaaccggc gtcccgtcta cctcacgcgc atgcccgaat ggcaacaccg caaacaagct
     7321 gacccaccag atcaacaaaa acacgataat gcccgtaacg agtcccatcg ctttaaaccc
     7381 tcaatacatg cacagtggtt ttcggtttaa tccctaaagt atggtcacaa aaccgtcgaa
     7441 ttccgatgcg cagctcttcg gcaataaaat gatcgtccag ccgctcttca aaagtcagct
     7501 cttcaagcaa atccaggatt tcatcataaa gcttgtcttc aatctgctct tcgccgggat
     7561 tatcaagatc cagaagccca agcatctcaa acttcggatc acccagaaat ttgccgcgcg
     7621 catccacagc caacgaaata tgcactgccc cggtatattg cagcttccgc cgcgccgcga
     7681 tggactggtg cgtcgccttg acgatccgtt tttgatccac agccagaatg tctgtttcaa
     7741 tatgatcaac aatctcagga ttgcccggcg ccaaacggat caccgaacca tttgaaggca
     7801 ccagcgcctt gggaacttga caatctcgtg caaacgccgc ctgtgcatca agctgcaccc
     7861 gctccccatg caccggcacg acgacctgcg gacgcaccca tcgcaacata tcggcaattt
     7921 catcgcggca cggatgcccc gacacatgaa taatgttttt cgtgtcgcgc ggcgtaataa
     7981 tattaacgcc tgcggcgctt aaattatttt tcacctgatt aatcgaaact tcattacccg
     8041 gaatagcgcg cgccgaaaaa atcacactat cccctttatt cagcttaaac cccttaaaga
     8101 ctccgcgtgc aacttttgcc aaagccgcgc gatattcacc ttgtgaaccg gttacaatca
     8161 ccacaacctt atcatccggc agatagccta aatcttcttc cgaaacaaaa tccggcaaat
     8221 ttttgtccaa aagtccacaa tccgccgcac acccgatcat tcggtgtaac gagcggccaa
     8281 taaccccgac ttgccgccca caagccttag ctgcctttaa aatactaatc acccggccga
     8341 tattggagga aaaagtcgtg acagcaatct tgccatcgca cttgcgaaac tcggcctcaa
     8401 gtcctttagc cacaacactt tccgaaccgc tgcgcccatc cacttctgca ttggttgaat
     8461 caccgatata ggccaagaca ccctcttcgc cggcccgctt aaaaatcgcc gcatccgtcg
     8521 ccgcacccgt caccggtgcc ggatcaagat tccaatctcc actatgcacc agcttaccat
     8581 gatccgtatc aataatcagc gaacatgtat cgggcacaga atgcgacacc ggtacaaaat
     8641 gaaccccgaa tgcaccaatt tgcacctgca cccctggcgt aataatttca atcttcaccc
     8701 gacgcacatt ctgttcttcc agcttcttgc gcagaacagc cgccgtaaac gggctggcat
     8761 aaattggaca ttgcaaccga tcccaaacat acgccaccgc gccaacatga tcctcatgtg
     8821 catgcgtaat aatcagcccc ttaagcgctt cccgccgctc ttccaaaaac gtgggatcag
     8881 gcaacagcaa atcaatcccg ggaaatcgat catcagcaaa accaatacca caatccaccg
     8941 ccaacatctg cccgtctgac acatacacat tcagattaac gccgaactgc tcgctgcctc
     9001 ccaacggaat aaaaaacaat ccatcctgac tcataagatc ccgcgccacg ctcatccgtg
     9061 catctcctga taaatcgcca gcagaccctt aagcgtcaga tcctggtcaa tcaaatcaat
     9121 tactcctgca tcctcaaaca acgccgcaag cccgcccgtt gccaaaacaa acggcttttc
     9181 accacccagc tctgcactaa tctgcccggt aatccgctca atcaaaccaa cataccccca
     9241 atacactccg gagcgcatag cctcagatgt tgattttcca atcgcctgcg caggcttacg
     9301 gacaccgatt ttaggcaact tcgaggccgc gctatgcagc gcattcaccg aaagatcaat
     9361 ccccggcgca atcgctccgc ctgcatacgc gccagacgca ttaatcacgt caaacgtcgt
     9421 ggccgtacca aaatcaatca caatcactcc acccgttcca atgcgctcac cataatgcat
     9481 attcaccgcc aaagcattca caagccggtc agcgccaaca tcctctggcc gttccatatc
     9541 aatcgtaatc ggtacatgat ccttggttat aaagaccggc acgaggtgca aatgcttttc
     9601 acaaaagcac cccaaatgaa aatgtgtttc cggcacaact gaactgataa caacatcatg
     9661 aatgttgata aaactaaggc catccatcac caaaatctga tgcaagaaca ccgcatactc
     9721 atcagcgcac cgccgcgcat aagtatgcaa ccgccaggca ccacggatga taccgccgtc
     9781 aaaaacagca aacacaatat tggtatttcc aacatcaatc gccagcagca tagtctcgat
     9841 cctttaaaaa cacatcgccc gctgagatgg tttgtaactg gttttgccta tcacgtacaa
     9901 ttaaatttcc caattcatca agactctcaa aggctccgac attgagcgcc ataccttttt
     9961 cgtaagtacg cgccagccat tccaggcgaa tatctgcaaa accatcacgc tgccaacgca
    10021 tataaagctg agcaatattg gcgagcaacg catcacgaaa cttatcacca tctacattga
    10081 gcactgcacc aatctcagga gcactggcca cattcacacc aataccgata accagccagt
    10141 ttaacgctcc cccggacaaa tcgctatcaa tcaaaatccc ggcgcatttt cggccatcaa
    10201 ccaacacgtc atttggccat tttaattgag cctttttacc aattgtctgc gccaacgcca
    10261 caccaatcat aatggaaatc tgcccgattt ttacgatctc gcatcgggga cgcaaaataa
    10321 aagacatcgc aagattacct tcttccgaaa gccatatacg cccatgccgt ccacgcccca
    10381 atacctgctg aatcgcctgc acaactgcgc cttcaggtac accatcgaca gccaaacgct
    10441 tgcattcatc ctgcgttgaa ttgagctttg taaaaacctg aatatcccaa tgcaaggccc
    10501 ctaccccgca aataacacgg acgccgcatt gaaagaactg gcaataaaaa cgctcggttt
    10561 aattacaaac ccaagcacaa acaaaataca gacaaataaa accacccggc gcgcaaacgg
    10621 aatcggcccg tcaaatacat cagcgggttc atcgaaaaac atcaccttaa tcacacgcag
    10681 ataataatag gccgcaacaa cagatgtaag taccccaagc gttgccagaa tgtaataccc
    10741 ttcggccact gcggcgttaa acaccaccag cttgccaaag aacccggccg ctggcggaat
    10801 accactcatc gaaaacatta agatagccat cgcataagcc aacccaggcg catgccgcga
    10861 cagccc
//
LOCUS       DAOSWR010000068        10859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3833_length_10859_cov_5.667253, whole genome shotgun sequence.
ACCESSION   DAOSWR010000068 DAOSWR010000000
VERSION     DAOSWR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3833_length_10859_cov_5.667253"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /locus_tag="PK513_06240"
     CDS             complement(<1..2597)
                     /locus_tag="PK513_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82082.1"
                     /translation="MRKPQKIQRSIFVFALALLMLVAFDARAEDVRARTGTHAQYSRL
                     VFDWDSHVEYSIDRAQNDRLLISFGRGAALDISFAKANPVANIESVDIISSDPLKVAL
                     SISADSRIRDLRAGNKVVIDIYNPPGAPPAEKPAPKIAEKHPATEKKTEHKTAHTEPV
                     EENHGKEKYKAPDQQQLHAATDAQDQERTQEGGEEQKTTRKLKMREQPTLITVSSTQN
                     FGMAAFEREGKLWLIGDKSNLLMKPQLSGPHVDQFSNIEEIEIENGKAFVMPAPKTHK
                     MSGEGGGILWRIKISPDLPTEEITSPIRVDVKRDEARSGKLLWPLKGARAVLELEDPL
                     TGRAIKVVTVDDAEQYTGPVREFVDFDVLHALFGLAIMPKVEDLELQIVRGGVEISRP
                     EGVTMVEDSLLASMTSRKIKRPDDKNTQTDMRVFDFKAWQLGGVNSMLENKRIILGGL
                     NDQPENARGENLLTLAKMYLSNAMSAEALGVLDFALLERPGLSESPQYKALLGAASAL
                     SSHNEAAYAHLSIEALKPFEEIQYWRAYVFADVGDWQQAADIFPQNVGALYDYPVLLQ
                     NRLVPQLAEIALRAGDLPRAQELLHMIDNNKKSMMPPQAAALAYLQGEAARQHHDIEK
                     TKKLWEPLATGADDLYRAKAGLALTRLKVNEGEITTKEAIDNLERLRYAWRGDQLEVQ
                     INYWLGRTYFEDGQYLKGLKIMRDAVSYDVGTPLGQRVAAEMADMFSDLFMGDDLDKV
                     SPMDSIALYDEFKELVPLDERGNQIIERLAEHLASADLLTRAGDLLQYQLDHRLQGAD
                     IYRIGVRLAAIRLLDNMPDKALAALNIAAAKLEELPEEMRTPRRYHDISLLRARALSR
                     KGRPDQALAL"
     gene            complement(2658..3467)
                     /locus_tag="PK513_06245"
     CDS             complement(2658..3467)
                     /locus_tag="PK513_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOO82083.1"
                     /translation="MIPEQKNDEDDEIEAGSYLSPAHNKRLEGREDSTALWLITFTDI
                     MALMLTFFVLLYSMSVPEEEEWENITSGLQSQFTKTFSTAQSKGPQDTISIDKIDFSK
                     AQDLSYLSSVLANVISKDERLKNIVLIPQSDHLIASVPEDLLFESGRASVSAKGKQVL
                     FSLGNTMSRIKNRIEIIGHTDPRPIQNTSGPFASNWELSLARAGSVATVLENAGYQRP
                     LTVRGLSSARYDSLPEEMSEEQRLSLARRVDIIIMEDDGNMRQFMPLDLPP"
     gene            complement(3464..4168)
                     /locus_tag="PK513_06250"
     CDS             complement(3464..4168)
                     /locus_tag="PK513_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82084.1"
                     /translation="MANQFLGLSLFVMLLSFFIILNAVSNFEETKSQPVLNSLALTFS
                     KKEALDLTPPGPALVKTMATQEGSTLDKIESLFKSQITSAKTKQNRLGTIMYVRVPFQ
                     EFERAILKSLTIPAAKAVPDFEENIDVLPMLVSLLETSREVTYKMDMLLNISQSPPVL
                     IGQNPERFNALNKSLSGVALKLEEAGLPKYQVTAGLKQGEEGIVELIFRRYQPFNPLG
                     GSEKPVETRPAAQGEL"
     gene            complement(4234..4833)
                     /locus_tag="PK513_06255"
     CDS             complement(4234..4833)
                     /locus_tag="PK513_06255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOO82085.1"
                     /translation="MSKAQQSYGREQVVQIINSVVSKIENVGGDATTTGIFKELKSLQ
                     RVIEEARADIGSTRPTDIKEKHIPIATDELDAVVESTAEATGTIMDCCDVIQEKAAET
                     GGEAADTINNEIIKIYEACSFQDITGQRITKVVATLKTIEEKVGKLIEILSHRLPIEE
                     GEATDGDEGKTIEERLLNGPQMPDKAITQDDIDKLLEDF"
     gene            complement(4865..5257)
                     /locus_tag="PK513_06260"
     CDS             complement(4865..5257)
                     /locus_tag="PK513_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82086.1"
                     /translation="MTVDKNMNVLIVDDYNTMLRIIKNLLKQLGFNNVDEATDGSMAF
                     DMVKQKKYGLIISDWNMEPMTGLDFLKSVRGSNGDYKNTPFIMVTAESKTENVVMAKQ
                     AGVNNYIVKPFNAETLKTKMQAVLGEFH"
     gene            complement(5414..6142)
                     /locus_tag="PK513_06265"
     CDS             complement(5414..6142)
                     /locus_tag="PK513_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HOO82087.1"
                     /translation="MKTAPKFRLLPVLIIIAMMAFSVRLVEFASGIKSLSSPAMAESA
                     PAPVHDTAENMPDSEKLIAADHVGDEEEHAADKKMAATHKEGETNEAEWRDSSYDELE
                     YSPVTREVFDDMVKRRKKLEAHERELQTREALLQAAEQELDRKFQELSTLRQEIEGLL
                     QKQSEQELERVQSLVKIYEGMKPKEAARIFDTLDLDILVTVMSRMSERRLSPILASMN
                     PERARTITIMLAEEKQLPALPLGD"
     gene            complement(6150..6692)
                     /locus_tag="PK513_06270"
     CDS             complement(6150..6692)
                     /locus_tag="PK513_06270"
                     /inference="COORDINATES: protein motif:HMM:NF039465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HOO82088.1"
                     /translation="MSSALLSLIMDFVVLAALVGTIYFAFRLSRSLDNFRKHRDEMKN
                     LIAELTRNINDAKDAIDALKATGNIAANNMEDVLYDSRRMAEELKMINETSDHLASRL
                     EKLASGAGSFSSRGAEEEYFDEPQAANTDQGPADTSPEPPSFFIQDRDFNEENDEDLV
                     SEAERELLQVLQKNKRTRKG"
     gene            complement(6689..7822)
                     /gene="fliM"
                     /locus_tag="PK513_06275"
     CDS             complement(6689..7822)
                     /gene="fliM"
                     /locus_tag="PK513_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOO82089.1"
                     /translation="MSDTELEEMSEEERAMMKEWETMANDDDDSGDADEALVEADAIP
                     AFDGLDDDDSGGTRILNQDEIDSLLGFDDDSAIGETSGVMALVNSAMVNYERLPMLDI
                     VFDRLVRLMSTSLRNLTTDNVEVSLDQVSTVRFGDYMNSIPLPAMLSVFKAEEWDNNG
                     LLVTDSALIYSIVDVLLGGRKGTPAIRVEGRPYTTIETKLVEKMVHVTLGDLSSAFDP
                     LSPVSFSLDRMETNPRFATITQSNNACVLARLRVDMGDRGGRVEILIPYATLEPIREL
                     LLQMFMGEKFGRDSIWESHLTQELYKTDIQLQAILGEKIMPLGEMLKWKVGTQIIFST
                     RTADDLELRCGSFPMFRGPVGQKQEHIAVRIEDYLEPEEDEGV"
     gene            complement(7930..8550)
                     /locus_tag="PK513_06280"
     CDS             complement(7930..8550)
                     /locus_tag="PK513_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOO82090.1"
                     /translation="MADDETDANEEAEDVNLLGEDGEDSEEGNKPIVLIALAGVVVLL
                     LGAGAGLYFTGMLDSVLGKSEPCVEEVDDHGEVIKECPEEDAHGDEHAAKDDGHGGGH
                     GGAGGNFIEIPTMLVNLSSNDGTPRYLRLTVQLELNSSADYAAVEAVIPRVVDQFQTY
                     LRELRVKDLRGSAGIYRLQMELLWRVNQAAAPVKVKDVLFQEILIQ"
     gene            8933..9676
                     /gene="flgF"
                     /locus_tag="PK513_06285"
     CDS             8933..9676
                     /gene="flgF"
                     /locus_tag="PK513_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOO82091.1"
                     /translation="MENSIYLALSRQMTLHTNMDMVANNIANMNTPGYRSQHLMFDEY
                     ISDPRGRTTDEAADDELSFVYNRGQYANTAPGTQSFTGNPLDVALAGPGFFGVQAPNG
                     EVMYTRAGQFQIDASGILTDPSGNPIADQGGASITIPPGSHDVAIDEKGMISNQDGQL
                     GQMMVVEFENQQKLEPYGSNLYRAPEPGTPAINTRVKQGQLENSNVQPVLEITEMMET
                     LRDYQSTQRILSTENERLRSVIQKLTGQN"
     gene            9700..10485
                     /gene="flgG"
                     /locus_tag="PK513_06290"
     CDS             9700..10485
                     /gene="flgG"
                     /locus_tag="PK513_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOO82092.1"
                     /translation="MRSLDIGATGMLAQQLNVDVISNNIANMTTTGHKRQRAAFQDLI
                     YQNIDRPGSTSSDAGTTVPSGLQLGLGVRAGAVYRQHGQGAIRITENALDVAITGEGF
                     FQIQLPNGDTGYTRDGTFQMNENGEIVTVQGFIVDPGITIPADAIDIDINESGEVLVS
                     LPGQTATSNVGQIQLANFVNPVGLEAIGDNLYVETDASGAPTTGIPGQDEFGRLRQGA
                     LEQSNVNVVEEITELITAQRAYEMNSTVISTSDEMLQTVSNLR"
     gene            10514..>10859
                     /locus_tag="PK513_06295"
     CDS             10514..>10859
                     /locus_tag="PK513_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82093.1"
                     /translation="MRKIKHRHSRQRPLALREWTMGFLAMIILSFSGIGLANAMASVS
                     LKHNSVIEGDMITLGHIFSGVDRNADRILGPAPQPGKDMLLNARTLMRIARAMDVNWR
                     PSSAADQVVLSRA"
BASE COUNT         2631 a         2658 c         2655 g         2915 t
ORIGIN      
        1 agagcaagcg cctgatcggg acggcctttg cgcgacaatg cgcgggcgcg gagtaaggaa
       61 atatcgtggt agcggcgggg cgttcgcatt tcttcgggaa gttcttcgag cttggcggcg
      121 gcgatattta aagccgcgag cgccttatcc ggcatattat cgagcaggcg aatggcggcc
      181 aaacgcacac caatgcggta gatgtcggcg ccttgcaagc ggtgatcgag ttgatattgg
      241 agcagatctc cggcgcgggt cagaagatcg gcggaagcga ggtgttcggc cagacgctct
      301 ataatctggt tgccgcgttc atccagagga accaattcct taaactcatc atagagcgcg
      361 attgaatcca ttggggagac cttgtcgaga tcgtcgccca tgaacaggtc agaaaacata
      421 tcagccattt ctgcggcgac gcgctggccg agcggcgtac caacatcata gctgacggca
      481 tcgcgcataa tttttagccc tttaagatat tggccatctt cgaaataggt gcgtccaagc
      541 cagtaattaa tctgcacctc cagctgatcg ccgcgccagg catagcggag tcgttcgaga
      601 ttatcgatcg cttccttggt tgtaatttct ccttcattga ccttcaggcg ggttaatgcc
      661 agccctgctt tggcgcggta aagatcatca gcgccggtgg ctaggggctc ccaaagtttt
      721 ttggttttct caatatcgtg atgctgacgc gcggcttcgc cttgaagata tgccagggcg
      781 gcagcctgcg ggggcatcat tgatttttta ttgttatcga tcatgtgcaa cagttcttga
      841 gcgcgtggaa gatctcctgc acgcagagcg atctcggcaa gttgcggcac caatctgttt
      901 tgcaacaaaa ccggataatc ataaagcgcc ccaacatttt gcggaaagat atcagcggcc
      961 tgttgccaat ctccgacatc agcaaaaaca taagcacgcc agtattgaat ttcttcgaaa
     1021 ggcttgagag cctcgattga taaatgcgca taggcagctt cattatggct gctgagcgca
     1081 ctcgctgcgc ctagcagcgc tttatattgc ggactttcgg acaggccggg gcgttcgagc
     1141 agtgcaaagt ccaatacgcc gagtgcttcg gcactcatcg cgttagacag atacattttt
     1201 gccaatgtta aaaggttttc gccgcgcgcg ttttcaggct ggtcattcag ccctcctaaa
     1261 attatgcgtt tgttttccag cattgagttc acgccaccaa gttgccaggc tttgaagtca
     1321 aatacgcgca tatcggtttg cgtattttta tcgtctgggc gttttatttt gcggcttgtc
     1381 attgatgcca ataacgagtc ttcgaccatg gttacccctt cggggcgaga aatctcaacg
     1441 ccgccgcgaa caatttgcaa ctccaaatct tcgacctttg gcataatggc caggccaaag
     1501 agggcgtgga gcacgtcgaa atctacaaac tcgcgcaccg ggcctgtata ttgttcagca
     1561 tcatcaacgg tgacgacttt aatagcgcgg ccagtcagag ggtcttcgag ttcaagaaca
     1621 gcgcgcgcac ccttaagcgg ccaaagcaat ttgccactgc gcgcttcgtc gcgctttaca
     1681 tcgacgcgaa tggggctggt tatttcttca gttgggagat cagggctaat tttgatgcgc
     1741 cagagaatgc cgcctccctc gccgctcatt ttgtgcgttt taggcgcggg cattacaaaa
     1801 gctttgccgt tttcaatttc tatttcttct atgttagaaa actggtcgac atggggacca
     1861 ctgagttgag gtttcatcaa gaggttgctt ttgtccccta tgagccagag ttttccttcg
     1921 cgttcaaaag ccgccatgcc aaaattttgc gtggaagaca cggtgattaa ggttggctgt
     1981 tcgcgcatct tgagcttgcg cgttgttttc tgttcttctc ctccttcctg agtgcgctcc
     2041 tggtcttgag catctgtggc agcgtgaagt tgctgctgat ccggcgcttt atacttttcc
     2101 ttgccatgat tttcttcaac aggttctgta tgcgccgttt tatgttcggt tttcttttct
     2161 gtggcagggt gtttttcggc aattttgggc gcgggttttt ctgcaggcgg tgcgccgggc
     2221 gggttgtaaa tatcaatcac cactttgttg ccggcgcgca gatcgcgaat gcggctatca
     2281 gcggaaattg aaagcgctac ttttaatgga tcagacgaaa taatatcaac agattctata
     2341 ttggccaccg ggttggcctt tgcaaaggat atatcaagcg cagccccacg gccaaagctt
     2401 ataagcaggc gatcgttttg cgcgcgatcg atgctgtatt caacgtgaga atcccagtca
     2461 aaaaccaggc gtgaatattg cgcatgcgtt ccggtgcgcg cgcggacatc ttcggcgcgg
     2521 gcatcaaatg ccaccagcat cagcagcgcc agcgcaaaaa caaagataga gcgttgaatt
     2581 ttttgcggct ttctcatcgt tttgagcgtg tcatatattg gttcaaagaa caaggatacg
     2641 cagcgtaaag aggtatttta agggggcaaa tccagcggca tgaattgccg catattaccg
     2701 tcatcttcca tgatgattat atcgacgcgc ctggctagcg acaggcgctg ctcctcactc
     2761 atttcttcag gcaagctatc ataacgggca ctggacaagc cgcgcacggt taaaggacgc
     2821 tgataaccag cattttcaag caccgttgcc acgctgccgg cgcgcgctaa cgacaactcc
     2881 caatttgatg caaacgggcc gctggtgttt tgaatcgggc gcgggtcagt gtgtccaata
     2941 atttcgatgc gatttttgat acgtgacatg gtgttgccaa gtgagaataa tacctgtttt
     3001 cctttggccg aaacgctggc gcgtccgctt tcaaaaagaa gatcttcggg cacagaggca
     3061 ataagatggt cactttgtgg aataaggacg atatttttca gacgctcatc tttcgatata
     3121 acgttggcca gaacagagct gagatagctg agatcctgag cctttgaaaa atctattttg
     3181 tcaatactga tggtatcttg cggcccttta ctctgggccg tggaaaaagt tttggtaaac
     3241 tggctttgca ggccgctggt gatattttcc cattcttctt cttcgggtac gctcattgaa
     3301 tataacagca cgaaaaaggt gagcatcaga gccatgatat ccgtaaaggt gatcagccac
     3361 aaagcggtgg aatcttctcg cccttcgagg cgtttattat gagccgggct taggtaactg
     3421 cctgcctcaa tttcatcgtc ttcatcattt ttttgctcag gtatcatagt tccccctgcg
     3481 cagcgggcct tgtttcaacc ggtttttcag accctccaag cgggttaaaa ggttgataac
     3541 gacggaagat taattcaacg attccttctt cgccctgctt taaccctgcg gtaacctgat
     3601 actttggaag gcctgcttct tcgagcttca gagcaacccc tgacaatgat ttattgagcg
     3661 cgttgaaacg ctcaggattt tgccctatca gtacgggcgg agattgggaa atattgagca
     3721 acatatccat tttatatgtc acttcacgac tggtttcgag cagagacacc aacatcggca
     3781 gaacgtctat attttcttca aagtcaggca cagcctttgc ggcaggaatg gtaaggcttt
     3841 tcaaaattgc tctttcaaat tcctgaaatg gaacgcgaac atacattatc gttcctaacc
     3901 gattttgttt tgtttttgca gatgtaattt gcgatttaaa taatgattca attttgtcca
     3961 gtgtgcttcc ctcttgcgtt gccatggttt taaccaacgc tggacctggc ggtgtgagat
     4021 cgagagcttc tttttttgaa aatgtcagcg ccagtgaatt aagaacgggt tgcgactttg
     4081 tttcttcaaa gtttgaaact gcgtttaata taataaaaaa cgacagcaac attacgaaca
     4141 gggaaaggcc aagaaactgg tttgccatga tttattatac gccgaaacgg gcgctaatga
     4201 caaagccgaa tgcacccgtg gcccctagtg cccttaaaaa tcttcaagca gtttatcaat
     4261 gtcgtcttgt gtgatggctt tgtccggcat ttgtgggcca ttcagcagac gctcttcaat
     4321 ggttttgcct tcatctccat cggtggcttc accttcttcg ataggaagtc tgtgactaag
     4381 aatctctatc agcttgccga ctttttcttc gatggtttta agcgtggcaa ccactttggt
     4441 aatgcgctgg ccggtaatgt cctgaaacga gcatgcctca tagattttta taatctcgtt
     4501 attaatggta tctgcggctt caccaccggt ctccgccgct ttttcctgaa taacatcgca
     4561 acaatccata atcgtgcctg tcgcctcggc agttgattca acgacagcgt caagttcatc
     4621 ggttgcgatc gggatatgtt tttctttaat atccgtagga cgcgtcgagc caatatccgc
     4681 ccgtgcctcc tcaattacgc gctgcaatga ttttaattct ttaaaaatgc ctgttgtcgt
     4741 ggcatcgccg ccgacatttt caattttaga tacgacagaa ttaataatct gaacaacctg
     4801 ttctctgcca tatgattgct gtgcttttga cattgctata cttccttact cgatttatgt
     4861 gtatttagtg aaactcgcct aaaacggcct gcattttagt tttgagcgtc tcggcattaa
     4921 acggtttcac gatatagttg ttcacgccag cctgcttggc cattacaacg ttctcggtct
     4981 tactttcagc tgtaaccata ataaaaggtg tgtttttgta atcaccatta gatccacgca
     5041 ccgatttgag aaaatcgagt cccgtcatcg gctccatatt ccagtctgaa ataatcagcc
     5101 catatttctt ttgcttcacc atatcaaaag ccatagaccc gtcggttgcc tcatcgacat
     5161 tattaaaccc cagttgtttg agaagatttt ttatgatccg caacattgtg ttgtaatcat
     5221 ccacgatcag gacattcata ttcttatcaa cagtcacttt tctctcctta ttgctacagg
     5281 ccttaatatt tggcacaccg ggaaagcaga tattatgcct tgaaatatta acagtttgta
     5341 agtgtaatga aaaacgcaga taaatctatg ttcagcttat acgaatacgg gggaatgtga
     5401 aggggaagtt gtgttaatct cccaagggta atgccggaag ttgtttttct tccgccagca
     5461 tgatggtgat tgtgcgggcg cgttcggggt tcatgctggc caatataggc gagagacggc
     5521 gctcagacat tcttgacatc accgtgacta aaatatcaag atcaagggta tcaaatatgc
     5581 gcgcggcctc tttaggcttc atgccttcat atattttcac cagactttga acacgctcaa
     5641 gctcttgttc ggattgtttt tgcagcaagc cttcgatttc ctgacgcaat gttgaaagct
     5701 cttggaactt gcggtctaat tcctgctcag cggcttgtag caaggcttcg cgtgtttgaa
     5761 gttctcgctc gtgcgcttcg agttttttac ggcgcttaac catatcgtca aagacttcgc
     5821 gcgttacagg agagtattcc aattcatcat agctagaatc acgccattca gcctcatttg
     5881 tttcgccttc tttatgcgtt gcagccatct ttttatcggc ggcgtgttct tcttcgtcac
     5941 caacatggtc tgcagcgata agcttttcgg aatcaggcat attttctgca gtatcatgca
     6001 caggcgcggg cgcgctttcg gccatggctg ggctgctcaa agattttata ccgctggcga
     6061 actcgaccag gcgcacagaa aacgccatca tcgcgatgat gatcaatacc ggcaaaagcc
     6121 gaaatttggg cgcggttttc atggagctat tatccttttc ttgttctttt atttttctgc
     6181 aagacttgaa gtagttcgcg ttcagcctca cttaccaagt cttcatcatt ctcttcgtta
     6241 aaatcgcgat cctgaataaa gaaagacggc ggttcgggtg atgtatccgc aggcccctgg
     6301 tcagtattgg ctgcttgcgg ctcgtcaaaa tattcttcct ctgcaccccg tgaagaaaaa
     6361 ctacccgcac cggaagcgag cttttcgagg cggctggcca aatgatcact ggtttcgtta
     6421 atcattttca attcttcggc catgcggcgg gaatcgtaca gtacgtcctc catattattt
     6481 gcggcaatat tgccggtggc tttgagcgcg tcaatcgcat ctttggcgtc attgatattg
     6541 cgtgtgagtt ctgcaatcag atttttcatt tcgtcgcgat gcttacggaa attatccaga
     6601 gaacgcgaaa gacgaaaggc aaaataaatc gttccaacca gcgcagccaa cacaacaaaa
     6661 tccataatca aactcagtaa cgcagaactc atacaccttc atcctcttcg ggttcgagat
     6721 agtcttcaat tcgtacagca atatgctcct gtttttgccc taccgggccg cggaacatgg
     6781 ggaaactccc gcaacgcaat tccagatcgt ctgcggtacg ggtactaaag ataatctgtg
     6841 tcccaacttt ccattttagc atttcgccca gcggcatgat tttttcaccc aaaattgcct
     6901 ggagctggat gtcggtttta tagagttctt gtgtcaagtg actttcccag atggagtcac
     6961 ggccgaattt ttcgcccatg aacatctgca atagcagttc acgaatcggt tccaatgtcg
     7021 catagggaat gagaatttcg acgcggccac cgcgatcgcc catatctaca cgcaggcgcg
     7081 caagcacaca tgcgttgttg ctttgcgtga tggtggcaaa acgcgggttg gtttccatgc
     7141 gatcgagtga gaagctgact ggcgagagcg ggtcgaaagc ggaagacaaa tcgcccagcg
     7201 tcacatgaac cattttttcg acaagtttgg tttcaattgt tgtataggga cgcccctcaa
     7261 cgcgtatagc aggagtacct ttgcgtccgc cgagcaacac atcaacgatg gagtatataa
     7321 gcgcagagtc ggtgaccaaa aggccattat tatcccattc ttcggccttg aacaccgaga
     7381 gcatcgcagg cagtggaatg gagttcatat aatcgccaaa acgcacggtg gatacctggt
     7441 caaggctaac ctcaacgtta tcggtcgtga gattacgcaa agacgttgac atcagacgca
     7501 ccaggcggtc gaacacgatg tcgagcattg gaagacgctc gtaattgacc attgcggaat
     7561 tgaccagcgc catgacgcca gaggtttcgc caattgcact atcatcatcg aagccaagca
     7621 aagagtcgat ttcatcttga ttgagtatcc gtgtgccgcc actatcgtcg tcgtcaagac
     7681 catcgaaagc cgggatagca tcggcttcga ccaacgcttc gtctgcgtcg ccggaatcgt
     7741 catcatcatt ggccatggtt tcccattcct tcatcatggc cctttcttct tcgctcattt
     7801 cctcgagctc ggtgtcgctc atgatcttat gttcttatgc cttgttttat catcatgttt
     7861 tcactttatc gtgcgcgtta tatttcgtgc ttgcaaatat catacctgtg ctaatcatta
     7921 tagcattaat tactgtataa gaatttcctg aaacagcaca tctttgacct ttaccggcgc
     7981 tgcagcctga ttaacccgcc aaagcaactc catctgcaaa cggtagatcc cggctgatcc
     8041 gcgtaaatct tttacacgca gttctcgtaa ataagtctga aactgatcaa ccacgcgcgg
     8101 gatgactgct tcgacagctg cgtaatctgc cgatgaattc aattcaagct gaacggtcag
     8161 gcgtaaatag cggggcgtcc cgtcattgga actgagattg accagcatag tagggatttc
     8221 gataaaattc ccgccagcgc cgccatggcc accgccatga ccatcatctt tggcagcatg
     8281 ttcgtcgcca tgcgcgtctt cttcagggca ctccttgatc acttcgccgt gatcatcaac
     8341 ttcttcgacg caaggctcgc tcttgcccag cacactatca agcatgccgg taaaatacag
     8401 cccggcgcca gcaccgagca gcaagacgac aacaccagcg agagcgatga ggacaatcgg
     8461 cttgttacct tcctcactgt cttcgccatc ctctccgaga agatttacgt cttcggcttc
     8521 ttcatttgca tcggtttcat catcagccat agcactattc atctttgttg ttggttatgt
     8581 tcacacagga cagaagggat acgcatttaa tttttgatta taaatcaaat cgcatacagc
     8641 gtctaacccg cgtaaacatc cttgctaaac tatacacaaa aggctctgcg gacctgtgga
     8701 aaacctacag gcaagttttg cccggcgcgc gcggcagttt tcgtcgtgac cccggcagcg
     8761 ccctcccgcc tgtctttagt atccaatcat attaacacat tgaaaaacat acgtaattga
     8821 ttctggcacg ggacatgcaa cgatgcccag tgaggggagc cgcacctaaa ataagcaggc
     8881 cccgcaaaag aaggtaagga ttaaaaattg agtaaggaca ggacagtaag ccatggaaaa
     8941 tagtatttat ctcgcgcttt cgcgacagat gaccttgcat accaatatgg atatggtagc
     9001 caacaatatc gccaacatga atacgcccgg ataccggtcg cagcatttga tgtttgatga
     9061 atatatttcc gatccgcgcg gcaggactac ggatgaggct gcagacgacg aactttcctt
     9121 tgtttacaac cgcggacaat acgcaaatac agcgccgggc acacaaagct ttacaggcaa
     9181 tccgctggat gtggcgctcg ccggcccggg ctttttcggg gtgcaggccc cgaacggcga
     9241 agtgatgtat acgcgggctg gccagttcca gattgatgcc agcggcattt taactgaccc
     9301 ttccggtaat ccgatagctg accaaggcgg cgcctctatt actattcctc cgggctcaca
     9361 cgatgttgct attgatgaaa aaggtatgat ttccaaccag gacgggcaac tcggccagat
     9421 gatggttgtt gaatttgaaa accagcaaaa actggagccg tatggaagca atctttaccg
     9481 cgcgccggag cccggaacgc ctgctataaa cacccgcgtt aaacaggggc agcttgaaaa
     9541 ttcaaatgta cagccggttt tggaaattac cgaaatgatg gagacgctgc gcgactatca
     9601 atcgacgcag cggattttaa gcacagagaa tgagcgctta cgctcagtca tacaaaaact
     9661 gacaggacag aattaaacgg attttaaagg agaaagacta tgagatcact ggatattggc
     9721 gcaacgggca tgctggctca acagcttaac gtcgatgtta tttccaacaa catcgccaac
     9781 atgaccacta cggggcacaa acgccagcgt gcggcttttc aggatttgat ttatcagaat
     9841 atcgaccggc ccggctcaac ctcttcggat gcggggacga cagtgccttc aggtctgcag
     9901 ctgggtctgg gcgtgcgcgc gggagctgta taccgccagc atggacaagg cgcgatccgc
     9961 atcacggaaa acgcgctgga tgtggcgatt acaggcgaag ggtttttcca gattcaattg
    10021 ccaaatggag atacgggcta tacgcgcgac ggaacgttcc agatgaatga aaatggcgag
    10081 attgtcaccg tgcagggctt tattgttgat ccgggtatca caattcctgc cgatgcgatt
    10141 gatattgaca ttaatgaaag cggcgaggtg ctggttagcc tgccggggca gactgcaaca
    10201 agtaatgtcg gacagattca actggcgaat tttgtgaacc cggtcgggct ggaagcgatc
    10261 ggtgataatc tttatgtcga aaccgatgct tcgggtgcac cgacgacggg cattccaggg
    10321 caggatgaat ttggccgcct gcggcagggc gcgctggaac aatcgaatgt gaatgtggtg
    10381 gaagaaatta ccgaattgat cacggcacag cgcgcctatg agatgaactc gacggtgatt
    10441 tcgacctccg atgaaatgct gcaaaccgta tcgaaccttc ggtagagcgc agtgaagttt
    10501 agggagtatc atcatgcgta agattaaaca cagacacagc agacagcgcc ctttagcgct
    10561 tcgtgagtgg acgatgggat ttttggcaat gattatcctt agctttagcg ggattggtct
    10621 ggccaatgcg atggcttcgg tatctttgaa gcataatagc gtaattgaag gcgacatgat
    10681 tacgctgggg catatatttt ccggtgtcga tcgtaacgct gacagaattt taggtcccgc
    10741 gccgcaacct ggcaaagata tgcttttgaa tgcgcgcact ctcatgcgca ttgcgcgggc
    10801 gatggatgtg aattggcgcc cttcttcggc ggctgatcag gtggttttga gccgcgcag
//
LOCUS       DAOSWR010000069        10620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3942_length_10620_cov_6.239565, whole genome shotgun sequence.
ACCESSION   DAOSWR010000069 DAOSWR010000000
VERSION     DAOSWR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_10620_cov_6.239565"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..291)
                     /locus_tag="PK513_06300"
     CDS             complement(127..291)
                     /locus_tag="PK513_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82094.1"
                     /translation="MFNNERTPVAIHMAYGSIMPGKMLTEPFQKMEKQRKIKEIVCII
                     ENKWQILTRP"
     gene            308..1696
                     /locus_tag="PK513_06305"
     CDS             308..1696
                     /locus_tag="PK513_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82095.1"
                     /translation="MSDSFKDITGKNQPARRTCRVAYTINNTQPVISTIIGDQPLFYM
                     DFDYSYEDEENPNFDPADMGIFESEIRLLKREIDSIEKLSAGFTAHDENKTEDFAENA
                     QDITSNKTGQNMLSIEPLLEMLRESRLATAYLATAEDHGITLRYSTQIETAHYDRTGM
                     LILINPNINKVDQVLLATQELRRHWQHRQGALINPLMFHPDNAVLINRAQIADLMVSM
                     IRVAWELQLSGEKSVWERIENSSMADLGRAFAREAFLDFRTVNNGQATAAVFESWFLS
                     ERCRTQDKNLINQMLADYKGYVFDLSEAEQNITPNLIAALGEMPFGKNYLAEHAVTIM
                     EDPIFTDVRDRSNANFLWFIKFERSFRDSEQELQTSSDLTAGGARSPAQNQRLGDRDD
                     AFFSAMPTGQYTGQSADIITLFEGDVGSKSKTGDKSAKNAKRLASKAGSGRSGNRGDL
                     SNVVYLRRSPGE"
     gene            1662..2756
                     /locus_tag="PK513_06310"
     CDS             1662..2756
                     /locus_tag="PK513_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82096.1"
                     /translation="MSFTSADPLVSENDHENYTHHTPVRFFDLQNGKCDMSNHDPYND
                     GLEIHEDFEQLDFEAAPFVDYDETPSLNSEAKLLSWAKDILSVSPSARIMLAEAKKQR
                     WIFSIEALEGPDFHIDVPQKRIVLSDQGLAISALGRSTYFKNSFLVSLIRALRDVWQE
                     KRHGGFDAQYACENVLMLERVRAADLDILAVLVGWELRGEGHGGLWRHLIDSEDGDLA
                     MRFSGYLERDPASLFNGRALAAAFSQWFRADRRIEACDHETLNYLDTLMQENAFEEVF
                     GARKLTPISIESLSCLPDRTAYLQGHGREIIADPLYAGLNDPINQAHYMQILYDLKVT
                     RVQDVPFRDAALAEKIFPGGEFTAEKDNIS"
     gene            2792..3664
                     /locus_tag="PK513_06315"
     CDS             2792..3664
                     /locus_tag="PK513_06315"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOO82097.1"
                     /translation="MSNQEQEPLKQFYIHLVSDATGTTLLGLARACLAQFEHVEPVQK
                     FWPLVRSERQLERVIARIKETPGPVIFTFVDHKMRHRLQDFCEELGVPCVAVLDPIIR
                     SLSSYLGEHAKGVPGLQHAMDDAYYKRVEAVDYAMRHDDGKTLKGLSKADVILVGVSR
                     TSKTPTSVFLARRGIKVANIPLVPGVEVPDAKLSFEKPMYVGLTASPQRLMHLRRTRL
                     HAKEQDAPPHIKDNAYLDEEAIEEEIRKARRLYSKKGWPVIDVTKRSIEETSAEIMAL
                     LQSRRDKLAKEGNE"
     gene            3661..4254
                     /locus_tag="PK513_06320"
     CDS             3661..4254
                     /locus_tag="PK513_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOO82098.1"
                     /translation="MTLILASQSKARQDMLHAAGLDFEISSADLDENAIMIAARQDGK
                     NPDEIAELLATEKARSVSAQTPGTLVIGSDQVLECEGQMLSKAANQNEAREKLKSLRG
                     KTHCLISAVSVVQNEKILWKTVQSAELTMHDFDDNFLQQYMECAGEALTRSVGAYELE
                     SLGAQLFDKIEGDYFTILGMPLLPLLKYLRENQGIGL"
     gene            4251..5084
                     /locus_tag="PK513_06325"
     CDS             4251..5084
                     /locus_tag="PK513_06325"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO82099.1"
                     /translation="MSEMIKAGVIGHPVSHSKSPLIHNYWIKTYGLNGSYEAIDAAPE
                     NLKSEIRRLTDEGYQGFNVTVPHKEAILELCDKVDLGAQNIGAVNTVVIQNGALRGMN
                     TDGFGFLHNIWDANPKFYFEEKTAVILGAGGATRAIVSALIGEYIGQIILLNRTRARA
                     ETIREEMGCMSDLIVVEDWDHRVELLARADLLINTTSLGMQGQPPLDLDLKALPSHAL
                     VNDIVYAPLQTDLLRSAQERGNPTVTGIGMLIHQARPAFKEWFGLLPDVTDELKKLVL
                     E"
     gene            5185..5859
                     /gene="coaE"
                     /locus_tag="PK513_06330"
     CDS             5185..5859
                     /gene="coaE"
                     /locus_tag="PK513_06330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943216.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOO82100.1"
                     /translation="MIVLGLTGSIGMGKSSAGAMLEYLGVPVHDADAEVHKLLHYGSE
                     AWYAIAAAFPYFSYPQIYGRKHFWNPYKDTIRYLKREALGKIIFEKPDEREKLEAILH
                     PLVRKAQNNFIRAQRGLGREIVALDVPLLFETGADMHCDYTINISAPAFIQEARVLAR
                     PGMNVEKFSAIVESQMPDGEKCARADFVVHSGLGRATMMRELKGVLAKIRAEQTPEED
                     EQGTAA"
     gene            5856..6545
                     /gene="dnaQ"
                     /locus_tag="PK513_06335"
     CDS             5856..6545
                     /gene="dnaQ"
                     /locus_tag="PK513_06335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HOO82101.1"
                     /translation="MKEIVLDTETTGMDPQNGDRIIEIGCVELMNHIPTGNTLQFYIN
                     PERDIPAEATAVHGITNEFVADKPVFSQVYSEFMSFIDGGKLVIHNAEFDMKFINWEL
                     GQVGHKAIGWGHVIDTLAIARKKFPGSPANLDALCRRFGIDNTERTYHGALLDSELLA
                     EVYLELLGGRQHGLGLASEKAKTEKSQQQTMLAKKSIRKARPHTPSAEELEAHKEFLE
                     KLKDPLWKKVS"
     gene            complement(6549..6671)
                     /locus_tag="PK513_06340"
     CDS             complement(6549..6671)
                     /locus_tag="PK513_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOO82102.1"
                     /translation="MKVLLTLVTLAVVPLVLSGCNTVEGMGKDIERAGESIQKL"
     gene            complement(6671..7228)
                     /gene="secB"
                     /locus_tag="PK513_06345"
     CDS             complement(6671..7228)
                     /gene="secB"
                     /locus_tag="PK513_06345"
                     /inference="COORDINATES: protein motif:HMM:NF004392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOO82103.1"
                     /translation="MSDQDAKDQSGAQTPGGMPINILAQYVRDVSFENPLAPDSLRAG
                     QTNPEMDINIGMDARKLPDETIDNLYEVVINLRAAALREEEPVFIMELQYGVTVALND
                     IPDDNHHPVLLIEIPRLAFPFIRQIVSELTVQGGYPPLNLNPVDFQALYMERFKDEIR
                     EAQEAANGKGKPAQKNAEKKEKEKN"
     gene            complement(7299..7778)
                     /locus_tag="PK513_06350"
     CDS             complement(7299..7778)
                     /locus_tag="PK513_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOO82104.1"
                     /translation="MPFIIIFVLIPFIELATFASVSEHIGIWTTLALAFLTAILGGLL
                     VKYQGLNTILSMRKAIDQGKMPLDEIFDGFCLVAAGALLITPGFVTDAIGFALLVPPV
                     RAALRYFIRTRTSWAVSGSTAEYETRQYRPHDGDIIEGDYERMDENEDQDRDEIKRL"
     gene            7949..8671
                     /locus_tag="PK513_06355"
     CDS             7949..8671
                     /locus_tag="PK513_06355"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HOO82105.1"
                     /translation="MPADLIVYALVAAGLVFWLRSVLGTRHGEEREHPNPFLAGEQDD
                     IKDQGLAAADRPAGMEENIIALTKEPRRNYGVDNKTAENGLIDISKADKSFDIDFFLE
                     GSQDAFAMIVESFAEGDRDTLKNLLSPQVFEAFEGAITEREKRKETETTDIHAIRKAY
                     VTQARLDGKMAYITVRFTAEETTVTRDENGEVIAGYPDKIVYMNDIWTFGRDIKSKDP
                     SWLVYETRSDIEDDNDRIPDTH"
     gene            8706..9320
                     /locus_tag="PK513_06360"
     CDS             8706..9320
                     /locus_tag="PK513_06360"
                     /inference="COORDINATES: protein motif:HMM:NF022835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HOO82106.1"
                     /translation="MPKALIFIFSLIIISFPFTTWAQEKIPDEDVKTWTQKAAASVMT
                     FGFHNYDKKLIENRKFFTLEGYQNFMIALSRSKTIENVHKNQSIINAKVMCFPRITKQ
                     EKESWTLMFPFSKTYSNSAGDDAFYSNAEMTVKYDENVENDMHLGIVQWVETTLSKEE
                     AEKCDPEEQKKAKIGQLQREIEWFEEQIEKNRKKIEKIKLEQKP"
     gene            9418..10518
                     /locus_tag="PK513_06365"
     CDS             9418..10518
                     /locus_tag="PK513_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HOO82107.1"
                     /translation="MGPVEKIVPEPNLKPASYTDLPGWGDDNFKTLIPAFTKSCERIL
                     KADPAKSFGTLKEAGTYGDWRPACYEFIALDTDDFERVQSFFETRFTPYQILSDNEET
                     GLFTGYYEASLKGSRVRGGPYQTPLYQRPEDLVMVQLGDFREDLKGRRIAGRVVDGNL
                     KPYEARPQIVNGNWPHNDKVLVWVDNPVDAFFVQIQGSGVVELAEGGTMRIGYAGQNG
                     HVYYAVGRELIKRGALTKENVSMQSIRQWLESSPAQADEIMNTNASYVFFKQIDGAGP
                     VGGEGVVLTAGRSLAVDHSLLSYGLPLWADIEAPVPGAGKLQRLLIAQDTGGAIRGPV
                     RGDVFWGYGSAAEALAGPMKSKGRYWALLPKH"
BASE COUNT         3004 a         2466 c         2435 g         2715 t
ORIGIN      
        1 cagtaaacac aagtaaaatt tatggacacg aaaaaacacg ggccttgacc cgtggggctt
       61 caccctttga gaaaatcaaa attttaacct ctcatcaata atgaataata atatgtaaac
      121 tgaatattaa ggccgagtta aaatctgcca tttgttttct ataatacata ctatttcttt
      181 gatttttcgt tgtttttcca ttttttggaa aggttccgtt aacatcttgc ccggcataat
      241 agaaccataa gccatatgaa tggcaacagg ggttctttca ttattaaaca tcaaagttgg
      301 gttcactatg agcgattcat tcaaggatat tacaggaaaa aaccagccag cgcgccggac
      361 atgccgtgtg gcttatacga ttaacaacac acaaccggtc attagcacga ttatcggtga
      421 tcagccgctt ttctatatgg attttgatta tagttatgaa gatgaagaaa atccaaactt
      481 tgaccctgcc gacatgggta tttttgaaag cgagattcgt ttgctgaaaa gggaaatcga
      541 cagcatagaa aaactttctg ctggttttac cgcgcacgac gaaaacaaaa ctgaagattt
      601 cgctgagaat gcacaagata taacatccaa caaaaccggt caaaacatgc tttccataga
      661 gccgttgctg gaaatgttgc gcgaaagccg tttagcaaca gcctatctgg ccaccgctga
      721 agaccacggc atcaccttac gctattccac acaaattgag acagcgcatt atgatcgtac
      781 tggtatgtta attcttataa acccaaatat aaataaagtt gatcaggttt tgctggcaac
      841 gcaagaattg cgacgtcatt ggcaacatcg ccaaggtgca ttaatcaatc cgttaatgtt
      901 tcatccggat aatgcagttc taatcaaccg ggctcaaatt gcagatctta tggtttctat
      961 gatccgcgtg gcatgggagc ttcagctttc cggtgaaaaa tcagtttggg agcgcattga
     1021 aaattcttca atggcggatt taggccgtgc attcgcgcgc gaagccttct tggatttccg
     1081 cacggttaat aatggtcagg caaccgccgc tgtatttgaa agttggtttc tatcagagcg
     1141 ttgccgcaca caagacaaaa acctcattaa tcaaatgttg gctgattata aaggttatgt
     1201 atttgacctg agcgaagctg aacaaaacat tacaccaaac ctgattgcgg cgttgggtga
     1261 aatgcccttc ggcaaaaatt acctggcaga acatgccgta accatcatgg aagatcctat
     1321 ttttaccgat gttcgtgatc gctcaaacgc caatttccta tggtttatca agtttgaacg
     1381 ttcgttccgc gattcggaac aagagttgca aacttcttct gatcttaccg caggcggcgc
     1441 tcgttctcct gcacaaaacc aaagattagg ggatcgagac gatgcattct tctcagccat
     1501 gcccacgggg caatacaccg gacaaagcgc cgacatcatc acactctttg aaggcgatgt
     1561 cggttcaaag agcaaaacgg gggataaatc tgcgaaaaac gccaaacgtc tggcatccaa
     1621 agcgggttca ggacgttcag ggaatagagg agatttatcc aatgtcgttt acctccgccg
     1681 atcccctggt gagtgagaac gatcatgaaa actacacgca ccatacccca gtccggtttt
     1741 ttgatctgca aaatggtaaa tgtgacatga gtaatcatga tccatataac gatggtcttg
     1801 aaattcacga agattttgaa cagttggatt ttgaagccgc accttttgtt gattacgatg
     1861 aaacgccatc tctaaatagc gaggccaaac tgttaagctg ggcaaaagac atattatctg
     1921 tcagtccgag tgctagaata atgttggccg aagctaaaaa acaacgttgg atattttcaa
     1981 tagaggctct ggaaggccct gattttcaca tcgacgtacc acaaaaaaga attgtgctta
     2041 gtgatcaagg cttggcaata tctgcactag gccgttccac ctactttaaa aattcctttt
     2101 tggtttcgct gatccgcgca ttgcgcgatg tctggcaaga aaaacgccat ggcgggtttg
     2161 atgcacaata tgcatgtgaa aatgttttaa tgcttgagcg cgttcgcgct gccgatcttg
     2221 atattctcgc cgtactggtg ggttgggaat tacgcggtga aggccatggt ggtctttggc
     2281 gtcacctcat tgactctgaa gacggtgatc tggctatgcg tttttctggc taccttgagc
     2341 gtgatccggc atcattattc aatggccgcg cgctggctgc tgctttttca caatggtttc
     2401 gtgcagatcg tcgtattgaa gcctgcgatc atgaaacgtt aaattatctc gatacgctga
     2461 tgcaggaaaa tgcatttgag gaagtattcg gagcccgtaa acttacgcct ataagtattg
     2521 aaagcctgtc ctgcttgcct gatcgcacgg cttatctgca aggtcatggc cgcgagatca
     2581 ttgctgatcc actctatgcc ggcttaaacg atccaatcaa tcaggcccat tatatgcaaa
     2641 ttctatatga cctcaaagtc acacgcgttc aggatgttcc attccgcgat gctgcgctgg
     2701 ctgaaaaaat cttccccggc ggagaattta ccgctgaaaa agataacatt tcttaaattg
     2761 cattcagtcc caatcccatt aatatgggcg catgagtaat caagagcaag aaccgctaaa
     2821 gcaattttat atccatctgg tttctgatgc gaccggaacc accttgttgg gactggcgcg
     2881 cgcatgtctg gcgcagtttg aacatgttga gccggtgcaa aaattctggc ccttggtgcg
     2941 ctctgaacgt caattagaac gagtcattgc tcgtattaag gaaacgccag gtccggttat
     3001 tttcaccttt gtagaccata aaatgcgtca ccgtctgcag gatttttgcg aagaacttgg
     3061 cgttccatgt gttgcggtgc ttgatcccat cattcgtagc ctttcttctt acctaggcga
     3121 gcatgccaag ggcgtaccgg gcctacaaca cgcaatggat gatgcgtatt acaaacgcgt
     3181 tgaggctgtc gattatgcga tgcgtcacga tgatggtaaa acgctcaagg gtctgtcaaa
     3241 agccgatgtt attctggtcg gtgtatcgcg cacatccaaa acgccgacgt ccgtatttct
     3301 tgcccggcgc ggcattaaag ttgcaaacat cccccttgta cccggtgttg aagtgcccga
     3361 tgcgaaactc tcgtttgaaa aaccaatgta tgttggcctc actgcttcgc cccagcgcct
     3421 catgcatctg cgccgcaccc gcctacatgc aaaagaacag gatgctccac cccatatcaa
     3481 agataatgcc tatcttgatg aggaagctat tgaggaagaa atccgtaaag cccgcagact
     3541 ttacagcaaa aaaggctggc ccgtcattga cgtgactaaa cgttcaattg aagaaacttc
     3601 cgccgagatt atggctctgc tgcaatcacg gcgtgataag cttgctaaag agggtaatga
     3661 atgactctga tcctcgcttc acaaagtaaa gcgcgccagg atatgctgca tgctgccgga
     3721 ctggatttcg aaatcagctc tgcagatctt gatgaaaatg cgatcatgat tgccgcgcgc
     3781 caggatggta aaaaccctga tgaaattgca gagcttctcg ccacagaaaa agcccgcagc
     3841 gtgagtgcgc aaacccccgg cacactggtc attggttcag atcaggtttt agaatgtgaa
     3901 ggccaaatgc tttccaaagc tgctaatcag aatgaggccc gcgaaaaact caaatccctg
     3961 cgtggtaaaa ctcactgctt aatttccgcc gtgagcgttg ttcaaaatga aaaaattctc
     4021 tggaaaaccg tgcagagtgc ggaattaaca atgcatgatt ttgacgataa ttttctgcaa
     4081 caatatatgg aatgcgcagg agaagcatta acccggtccg taggcgctta cgaacttgaa
     4141 tcattgggcg cgcagctttt cgataaaatc gagggcgatt atttcaccat tttaggaatg
     4201 ccacttttgc cgcttttaaa atatctacgc gaaaaccagg gtataggatt atgagcgaaa
     4261 tgatcaaagc cggcgtgatc ggtcatccgg tcagccattc caaatctcct ctcatccata
     4321 attactggat taagacttat ggtcttaatg gcagctacga ggcaattgac gctgcgccgg
     4381 aaaatcttaa aagtgaaatt cggcgtttaa ccgatgaagg ctatcaaggg tttaacgtca
     4441 ccgtgccgca taaagaagct atattggaat tgtgcgacaa ggttgatctg ggagcgcaaa
     4501 acatcggcgc agtcaatacg gttgttatcc aaaatggcgc attgcgcggc atgaataccg
     4561 atggctttgg tttcttgcac aatatatggg acgccaatcc caaattctat tttgaagaaa
     4621 aaacagccgt tattctaggt gcaggcggag caacgcgcgc aatcgtcagc gcattaatag
     4681 gtgaatatat tggtcagatt atcctgctta atcgtacccg tgcgcgcgct gaaaccatcc
     4741 gcgaagaaat gggatgtatg agcgatttga ttgtcgttga agattgggat catcgcgtcg
     4801 aacttttggc cagagccgat ttactgatca atactacgtc attaggcatg caaggccagc
     4861 cgccgctgga tttggattta aaagcattgc cttctcacgc gctggtcaat gatattgtct
     4921 atgcgccgtt gcaaacagac cttttacgat cagcgcaaga gcgcggtaat ccgactgtaa
     4981 cgggtatcgg catgctcatc catcaagctc gcccggcctt taaggaatgg ttcggcctct
     5041 tgcctgacgt gacagatgaa ttgaagaagt tggtgttaga atgacgtcat tgcgaggaag
     5101 caaagctgac gaagcaatcc ataaatattg tacttgtggc actggattgc ttcacttcgt
     5161 ttacaaggac aatgagggga aacaatgatt gtccttggcc taacaggttc cattggaatg
     5221 ggcaaatcca gcgctggcgc gatgctggaa tatctcggcg taccggtgca tgatgctgac
     5281 gccgaagtgc acaaactttt gcattatggg agtgaagcct ggtacgccat tgctgctgcc
     5341 ttcccgtatt tttcataccc acagatttac ggacgtaagc atttctggaa tccatataaa
     5401 gatacaattc gatatttaaa acgcgaagcg ctgggaaaaa tcatatttga aaaaccggat
     5461 gaacgcgaaa agcttgaagc cattctccat ccgctggtgc gcaaagcgca aaataatttt
     5521 attcgtgctc aacgggggtt aggccgcgaa atagtcgcgc tggatgtgcc gctcctgttc
     5581 gaaaccggtg ctgatatgca ttgtgattac accatcaata tctctgcgcc cgcctttatt
     5641 caggaagccc gcgttctagc tcgccccggc atgaatgtcg aaaaattttc tgctattgtc
     5701 gaaagccaga tgccggacgg cgaaaaatgc gcccgcgctg attttgttgt acatagcgga
     5761 ctggggcgcg ctacgatgat gcgggaatta aagggtgttc tggctaaaat cagagcagag
     5821 caaacccccg aagaggatga acaaggcacc gccgcatgaa agaaatcgta ctcgacaccg
     5881 aaaccacggg catggacccg caaaatggcg atcgcatcat tgagatcggc tgcgtcgagc
     5941 tgatgaatca tattccaaca ggtaatacgc tccagttcta tattaatcct gagcgcgata
     6001 ttcctgccga ggccacagct gtccatggca ttaccaatga gtttgtcgct gacaaacccg
     6061 ttttcagcca ggtctatagc gaatttatga gcttcataga cggtggcaaa ctggtcatcc
     6121 acaatgccga attcgatatg aaattcatca attgggagct ggggcaggtt ggccataaag
     6181 ccatcggctg gggtcatgtt atcgatacgc tagccatagc acgtaaaaaa ttccctggtt
     6241 cacctgcaaa tctggatgca ctgtgccggc gttttggcat tgataacacc gagcgtacct
     6301 accacggggc gctcttagac tcggaacttt tggcggaagt gtatctcgaa ctactcggcg
     6361 gacgccagca cgggctaggg cttgcttctg aaaaagcaaa aaccgaaaaa tctcagcaac
     6421 aaaccatgct ggcaaaaaaa tcaatacgca aagcacggcc acatacgccg agcgctgaag
     6481 aattggaggc gcataaagaa tttttggaaa aactcaaaga tccgctttgg aaaaaagtaa
     6541 gctaagccct agagtttttg gatagattct ccggctcttt ctatatcttt gcccatacct
     6601 tcaacggtat tacaaccgct taaaaccaac gggacaaccg ccaacgttac caaggttaat
     6661 aaaactttca tcagtttttc tccttttctt ttttctcagc gttcttttgg gcaggcttgc
     6721 ccttaccatt tgccgcttct tgcgcctctc taatttcgtc tttaaagcgc tccatataaa
     6781 gtgcctgaaa atcaaccggg ttgaggttta gtggcggata tccgccttgc actgtcagtt
     6841 cgctgacaat ttgacgtata aatgggaaag ccaaacgcgg aatttcaata agaagaactg
     6901 gatgatgatt gtcgtccgga atgtcgttaa gggcgaccgt gacgccatat tgcagctcca
     6961 taataaaaac aggttcttct tcacgcaatg cagcggcgcg caaatttata acaacctcat
     7021 aaagattatc tatcgtttca tccggcagct tgcgtgcatc cattccgata ttaatatcca
     7081 tttccggatt cgtctgcccg gcgcgcaaag aatccggcgc cagtgggttt tcaaaggaca
     7141 catcacgcac atattgcgcc agaatattaa tcggcattcc acccggggtt tgcgcgccag
     7201 attggtcttt agcgtcttga tcactcataa aaataactcc aaatttaaaa tcaacctcgt
     7261 gattaccacg tttaactata tgcttcaatt aagcactgtc acaggcgctt aatttcatcc
     7321 cggtcttggt cttcattttc atccattcgc tcataatcgc cttcaatgat gtcaccatcg
     7381 tgcgggcgat actggcgcgt ttcatattcg gctgtgctgc ctgaaacagc ccagcttgta
     7441 cgcgtgcgaa taaagtaacg cagcgccgcc cgcacaggcg gcaccagcag tgcaaacccg
     7501 attgcgtcag ttacaaaacc cggcgtaatc agcaaagcgc ctgccgcgac cagacaaaag
     7561 ccgtcaaaaa tttcatccag aggcattttg ccctgatcta ttgctttgcg cattgaaaga
     7621 attgtattca aaccctgata tttaaccagc aatccaccga gtattgcggt caaaaacgcc
     7681 agagctaacg tcgtccatat tcctatatgc tcactgacgc tggcaaaggt cgcaagctct
     7741 atgaacggaa tcagcacaaa aataataata aacggcatga accttcctcg ataatagctt
     7801 aggccaatct atgtgagcac cttctaaaac acaaataaga aatcctaaag cgaaaatccc
     7861 gtctttttag cgcttttatt ggacaaaacg tgataaaatg aacatattgc ttgagtaaat
     7921 atgaatttta acaagggaag gctccttcgt gcccgctgat ttgattgttt atgccttagt
     7981 agctgccggt cttgtattct ggctgcgtag cgttttggga acgcgtcacg gtgaagaacg
     8041 tgagcatcca aacccgtttt tggctggtga gcaggacgat attaaggatc aaggccttgc
     8101 cgctgcagat cgccctgccg gaatggaaga aaatattatc gcgctaacga aagagccgcg
     8161 ccgcaattac ggcgttgaca acaagacggc tgaaaatggg ttgatcgaca tttctaaagc
     8221 cgataaaagc ttcgacattg attttttcct cgaagggtcg caagacgctt tcgcaatgat
     8281 tgtggaatct tttgcagaag gagaccggga cacgctcaaa aatcttttaa gcccacaggt
     8341 ttttgaggct tttgaaggtg cgattactga acgtgaaaag cgtaaagaaa cggaaaccac
     8401 tgatattcat gctatccgaa aggcctatgt aacccaggca cgccttgacg gtaaaatggc
     8461 ctacatcacc gtgcgtttta cagccgaaga aaccactgta acgcgtgacg aaaacgggga
     8521 agttatcgca ggctatcctg acaaaatcgt ttacatgaac gatatctgga cattcggtcg
     8581 cgatataaaa tccaaagatc caagctggct ggtctatgaa acccgcagcg acatcgaaga
     8641 cgataacgac cgcattcccg atacgcatta aaattcaaaa cacttccata taaaggatct
     8701 taacgatgcc aaaagcgctt atttttatct tttctctcat tattatctct ttccccttta
     8761 caacgtgggc acaagaaaaa attcctgatg aggatgttaa aacatggact caaaaagctg
     8821 ccgcatcagt aatgacattt ggatttcata actatgataa aaaactcatc gaaaatagga
     8881 agtttttcac tcttgaggga taccaaaact tcatgatcgc gctatctcgt tctaagacaa
     8941 tagaaaatgt tcataaaaat cagtccataa taaatgcaaa agttatgtgt tttcctagaa
     9001 tcacaaaaca ggaaaaggag tcatggaccc tgatgtttcc tttttctaaa acttacagca
     9061 atagtgcagg agatgacgca ttttattcta acgcagagat gacagtaaaa tacgatgaaa
     9121 atgtagaaaa tgatatgcac ttagggatag tgcagtgggt tgaaaccaca ctcagcaaag
     9181 aagaagcaga aaaatgtgat cctgaagaac aaaagaaagc aaaaattgga caactgcaac
     9241 gcgagataga atggtttgaa gaacaaattg aaaaaaaccg taaaaaaatt gaaaaaatta
     9301 aacttgaaca aaagccttaa gaaggaaaga acttacctta tcggtaatac accgtatttt
     9361 tctaatacgg ttggcgtgtt ctttgcatct tccctatgct ttttgctagt agcatgtgtg
     9421 ggtcctgttg aaaaaatagt accagagcct aatctcaaac ctgccagcta tactgatttg
     9481 ccgggctggg gcgacgacaa ttttaaaaca ctaatcccgg ccttcaccaa atcctgtgag
     9541 cgcattctca aagccgatcc tgccaaatcg tttggcactt taaaagaggc tggaacttat
     9601 ggcgattggc gaccagcgtg ttatgaattt atagctttag acacggacga tttcgagcgc
     9661 gttcaaagct tttttgaaac tcgttttacg ccctatcaga tcctatctga taacgaagaa
     9721 acagggctgt ttacaggtta ctatgaagcc tctcttaaag gctcacgcgt acgcggtggc
     9781 ccataccaaa caccgcttta ccagcgcccc gaagatctgg tcatggtcca actcggtgat
     9841 tttcgcgaag atctcaaagg tcgccggatc gcaggacgtg ttgtcgatgg caatctgaaa
     9901 ccctacgaag cacgccctca aatcgtaaac ggcaattggc cccataacga taaagtactg
     9961 gtctgggtcg ataacccggt tgatgcgttc ttcgtacaaa ttcaaggctc gggtgttgta
    10021 gaattggccg aaggcggaac catgcgtatc gggtatgccg gacaaaacgg ccatgtttat
    10081 tatgccgttg gccgcgagct gattaaacgc ggcgcattaa ccaaagaaaa tgtttctatg
    10141 caatccatcc gccagtggct ggaatccagt ccagcccaag ctgatgagat catgaatacc
    10201 aacgcctctt acgtattttt taaacagatc gatggcgccg ggccggtagg cggcgaaggc
    10261 gtcgtgttga ccgctggccg ttcgctggcc gtagaccact cattattatc ctatggcttg
    10321 ccgctttggg ccgatattga ggcccccgtg cccggcgcgg gcaagctcca gcgtttgtta
    10381 atcgcgcaag ataccggcgg tgccatccgc ggccctgtgc gcggcgatgt gttctggggt
    10441 tatggcagcg cggcagaagc cctggccgga cccatgaaat caaaaggtcg ctattgggct
    10501 ctgttgccaa aacattaata cggcatcggg aagtctcgat aaacgctttc aatctctctc
    10561 agaacatcat ctgaaagctc taaattaacc gacgcgatat tcgatttaag ctgctccatg
//
LOCUS       DAOSWR010000070        10530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_3987_length_10530_cov_5.548258, whole genome shotgun sequence.
ACCESSION   DAOSWR010000070 DAOSWR010000000
VERSION     DAOSWR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10530
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_10530_cov_5.548258"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK513_06370"
     CDS             complement(<1..257)
                     /locus_tag="PK513_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO82108.1"
                     /translation="MPYLLLLLGLIIGLYALYRFFINADVAQVKAAFILAIMVVLCAA
                     LFTLAVTGRLPAALGILVAVAPLAAGLWKARRDEHSETLEPG"
     gene            308..2086
                     /locus_tag="PK513_06375"
     CDS             308..2086
                     /locus_tag="PK513_06375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOO82109.1"
                     /translation="MKNTKEKISALRAFMAEQGLDGFIVPKADEYQGEFVAPYAERLK
                     WLTGFTGSAGVAIVLQNKACVLTDGRYRIQITGQIDNDIFAAGDSIKTGLHGWLLTHA
                     EKGSKIGYDPWLHTPNQVQKLKEECPEIELIPVKHNLIDEIWLDQPEKPRGRVEIFPQ
                     SLAGASLKEKKARVAENIKAAGAQAAMITLPDSIAWLLNVRGSDIEYIPSVLSYAVVF
                     ADEAEPVWWIVDQAKVGADVRAYGDGAFKLATLEDLQSIKASPIMFDCAHSPEYFHTQ
                     LLAQGQEIIDKKDPCIDLKALKTDAEVEAIKKAHIIDALALTKFLHWLDKTVQRKEIT
                     ETDVADQLQKFRAENSAYKGPSFATISGFGSNGAIVHYRADDQTNKTLEEGSLLLVDS
                     GGQYCENDAAGTTDITRTIVIGQPSDEMRRNFTLVLKGHIALAMAKFPAGTTGAQIDT
                     LARQPLWNEGLDYAHGTGHGVGCYLAVHEEAASISPKGTESFKSGMLISNEPGYYKDG
                     AYGIRIENLVLVKEAGQCADTGGQMLRFETVSFVPIDRRLIDVKMLNVQERDWLNDYH
                     AKLYEHLHEGLSNEVSDWLHTATLPV"
     gene            complement(2265..3473)
                     /locus_tag="PK513_06380"
     CDS             complement(2265..3473)
                     /locus_tag="PK513_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82110.1"
                     /translation="MNISFSLSRRRIYRPGFRHFVWLGLVALTFSGILSPFAQAQNWH
                     NYSAEGSLFDVRILDNVEETALNFRINSKTVAQSGQAVAVSDQRPYKDSVTHYIVRFD
                     QSLGVPVHDSYIRRFIIEDLDVFASYYSNMNGKVHSRENFLYQYGVPGGEIKISYDDP
                     NMGRQHIRMRTFFLGISKIQQIVMGDEGKMNSFAARKFFDSLYAKDGYKVNGGSIQKE
                     WPAHTSPSGMFTAFLPDKVSPYVPANVDIIQTDKFERMSFRFYDPIWREMIFYNIYGH
                     KLPKKKLNFLDVEAFLEKEYVSRHLSEYAANLRMDRFMNNNLPVIETSYPIEPPKNYP
                     YVDKVRLRTQFLGDSIIVHEIIAASHFVTSKFMDYVMQSVVFHPGQQLQESLDQNESE
                     QQEEDQPVSE"
     gene            complement(3555..3761)
                     /gene="rpsU"
                     /locus_tag="PK513_06385"
     CDS             complement(3555..3761)
                     /gene="rpsU"
                     /locus_tag="PK513_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOO82111.1"
                     /translation="MVSVSVRDNNVDQALKVLKKKMQREGIFREMKLRRHYEKPSEKK
                     AREQSESVRRARKMDRKRRERDGF"
     gene            complement(3903..5513)
                     /locus_tag="PK513_06390"
     CDS             complement(3903..5513)
                     /locus_tag="PK513_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO82112.1"
                     /translation="MTETLLQIKNLSVDFKTQGGGLRAVDDVSFEIQKGETMALVGES
                     GSGKSVTALSIMQLLPYPRAVHPDGGAIQFNGEELVGKGESFMRTVRGRKIGMIFQEP
                     LTALNPLHTIEKQISEVLFLHQKMNKPQARARVLELLELVGLEGLKTRLNAYPHELSG
                     GQRQRVMIAMALANDPELLIADEPTTALDVTVQAQVLELLKDLQKRLGMALLLITHDL
                     TIVEKMSDHVCVMQRGKIVEQNETAKLFAKPQHEYTKMLLSAQPKGDAIPADMNAPVI
                     LEGSDIKIYFPKAKNLFGKPLDWVKAVDNIDIVAKKGQTIGVVGESGSGKTTLGLGLL
                     KLLGAQGRIVFEGRDISSHNAKEMLPLREEMQIVFQDPFGSLSPRMSVEQIIGEGLKI
                     HRSALSREERDALVVQALEDVHMDPETRHRYPHEFSGGQRQRIAIARALSLHPKFIVM
                     DEPTSALDLSVQAQIVDLLRELQERHNISYMFISHDLRVVKAMAHDLIVMKEGRVVEA
                     GPAKAIFGNPQEDYTKALLAAALDLKTA"
     gene            complement(5510..5944)
                     /locus_tag="PK513_06395"
     CDS             complement(5510..5944)
                     /locus_tag="PK513_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82113.1"
                     /translation="MSEAIFGFLGVIIGSFIPWVREWLNEKRLREKHAAYLAVQIICV
                     LDEYVGKCCEVVGDDGTIMGQPDYEEFFEARQDGYANLGLDAIELKEALRKKYEIPDE
                     NFEINPDWNARKFLEKKRDGIEERRKQRASHFHEMTKEIRAT"
     gene            complement(5997..7295)
                     /gene="argH"
                     /locus_tag="PK513_06400"
                     /pseudo
     CDS             complement(5997..7295)
                     /gene="argH"
                     /locus_tag="PK513_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(7282..7458)
                     /locus_tag="PK513_06405"
     CDS             complement(7282..7458)
                     /locus_tag="PK513_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82114.1"
                     /translation="MAIPTGFVAAVLFIPLYFSSYLFYTLGISIWFWMVACTFIVGLY
                     EMLRQEFKKENEQK"
     gene            complement(7558..7851)
                     /locus_tag="PK513_06410"
     CDS             complement(7558..7851)
                     /locus_tag="PK513_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO82115.1"
                     /translation="MPKQYYVYILSKARNSTFYVGITSDLPRRIWEHKNEVADGFTKK
                     YGIKTLVYYEVHDDPETAIRREKRLKKWPREWKMKIIEEMNPDWKDLYEDICS"
     gene            complement(7899..9149)
                     /locus_tag="PK513_06415"
     CDS             complement(7899..9149)
                     /locus_tag="PK513_06415"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOO82116.1"
                     /translation="MSKGKIVLAYSGGLDTSVILKWFIEEGYEVIAYIADVGQEEDFE
                     AAREKALKIGAAKVYVEDLKEEFVTDYIFPAFKSQAVYEGRYLLGTSVARPIIAKRQI
                     EIAEKEGAQFVGHGATGKGNDQVRFELSYYALKPDIKVLAPWKMPEFLTRFEGRQDML
                     NYCDQYGIPVSATRKKPYSEDDNLLHISHEAGILEDPNAPCAEDVYSRTSTIKDWPDE
                     PELISIEFKAGVPVKVEAEGKTVTGALALFQFLNELGAKHGIGRLDMVENRFVGIKSR
                     GIYETPGGTILHNAHRDLEGLAMDREVMKLRDTLSLKVAELIYNGFWFSPEFELLMTT
                     MDKAQETVNGTVQVQLFKGSAYPVARTSPNALYDPAQSSMDEEGGYDQKDAEGFIKIN
                     AVRLRNNTKVRGETPASKPSKAAA"
     gene            complement(9354..9671)
                     /locus_tag="PK513_06420"
     CDS             complement(9354..9671)
                     /locus_tag="PK513_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82117.1"
                     /translation="MTRYLDDTNRQDGDKEGVSAFFQGCQEIAASSVTSAKNYFTKVP
                     VDDCGEAGKVPNYKKFVVRRVLDFTTAASLAGMIYVGSSLDNKTNPECSSNDQPVPTL
                     SAG"
     gene            complement(9859..>10530)
                     /locus_tag="PK513_06425"
     CDS             complement(9859..>10530)
                     /locus_tag="PK513_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO82118.1"
                     /translation="SVKDTANVVSRYVDGVMIRMSDDDVLKDFAGHCSIPVINGMTDK
                     THPCQIMADMLTIEEKLGSIAGRKIAWFGDCNNVSNTFVQAAPIFGFELALSVPLELK
                     PDDLTDYVAYFEDPEAAARDADVLVTDTWVSMGQEGKAIDKFRHYQVTPALMAKAQDS
                     AIFMHCMPVHKDEEVAQGVLDSPANVIYDEAENRLHAQKAILAWCLEEAGVSVSQSSF
                     NFGNA"
BASE COUNT         2673 a         2841 c         2312 g         2704 t
ORIGIN      
        1 cccggttcaa gcgtttcaga atgttcatcc cggcgggctt tccacaggcc ggccgccagc
       61 ggcgccaccg cgaccaaaat gcccagcgcc gcgggcaatc gcccggtcac agccagcgta
      121 aacagcgctg cacaaagcac caccataatg gcgaggataa aggcggcctt aacctgcgcc
      181 acatcggcat tgataaaaaa ccggtacagc gcatacaggc cgattataag tccaagtaaa
      241 agaagtaaat atggcatagc ttcaaaaaca ctagcaaaac atatacgcct gcgccagtat
      301 aagaagcatg aaaaatacaa aagaaaaaat aagcgcccta cgtgctttca tggcagagca
      361 agggcttgac ggttttatcg ttcccaaggc cgatgaatat cagggcgaat tcgttgcccc
      421 ttacgccgag cgcctgaaat ggctcacagg ttttaccggg tcagccggtg ttgctattgt
      481 gttacaaaat aaagcctgcg ttctgactga tgggcgctac cgcatccaga ttactgggca
      541 gatcgacaac gatatttttg ccgccggcga cagcattaaa accggtctgc atggctggtt
      601 gcttacacat gctgaaaagg gctctaaaat aggctatgat ccttggcttc ataccccaaa
      661 tcaggtgcaa aaacttaaag aagaatgccc ggagattgaa ctgattccgg ttaagcacaa
      721 cctgattgat gaaatttggc tggaccagcc ggaaaaaccg cggggccgtg tcgaaatttt
      781 tccgcaaagt ctggccggtg catcgttaaa ggaaaaaaag gcacgtgtgg ctgaaaacat
      841 aaaagcagca ggcgctcagg cggccatgat cactttgcca gactctatcg cctggctgct
      901 caatgttcgc ggcagcgata ttgagtacat tccctccgtt ttatcctatg cggtcgtttt
      961 tgcggatgag gcagagccgg tttggtggat tgttgatcag gccaaagtcg gtgcggatgt
     1021 acgcgcttat ggcgacggcg ctttcaaact cgccactctt gaggacttac agagcataaa
     1081 ggccagccct atcatgtttg attgcgccca cagcccggaa tatttccata cacaattatt
     1141 ggcgcaaggg caggaaatca tagacaagaa agatccatgc attgatttga aagctctgaa
     1201 aacggatgca gaagttgaag caataaaaaa agcgcatatc attgatgcat tagcgctgac
     1261 gaaatttctc cattggcttg ataaaactgt gcagcgcaag gaaatcaccg aaacggatgt
     1321 tgccgatcag ttgcaaaagt ttcgcgctga aaactcagca tacaaagggc caagctttgc
     1381 aaccatttcc ggctttggtt ccaatggcgc gattgtacat tatcgcgccg atgatcagac
     1441 aaataaaacg ctggaggagg gcagcctgct gcttgttgat agcggtgggc agtattgtga
     1501 aaatgatgcc gcaggaacga ccgatattac gcgcactatc gtaattggtc agccgagtga
     1561 tgaaatgcgg cgtaatttta cactggttct caaggggcac atcgcactgg ctatggcgaa
     1621 gtttcccgct ggcacaaccg gtgcgcagat cgatacgctt gcgcgccagc ctctttggaa
     1681 cgagggtttg gattacgcac acggtacagg ccacggagtt gggtgctatc tcgccgtgca
     1741 tgaagaagcc gcaagtatct caccaaaagg aacggaaagc tttaaatccg gcatgctcat
     1801 ttcaaatgag ccgggatatt ataaggacgg cgcctatggt atccgcattg aaaacctggt
     1861 gctggtgaaa gaggcagggc agtgtgccga caccggcgga caaatgctca ggtttgaaac
     1921 agtgagtttc gtgccaatag atcgccgctt gattgatgta aaaatgttaa atgtgcagga
     1981 gcgtgattgg ctcaatgatt accatgcaaa actgtacgag catttacatg aaggcttatc
     2041 aaacgaagtg tctgattggc tgcatactgc aacgttgccc gtttaatctt tttcaggttt
     2101 aattcctcgc cgcttgcggc gtaatcgtca tcgcgagcgc agcgaagcga tccagtgcca
     2161 caaggataaa ttctggattg cttcaccgcc tttggcggtt cgcaatgacg cgatacctca
     2221 cggcttgcgg cgatattgtt cattggaaag caggattgcc cggattattc agaaacgggt
     2281 tggtcttctt cttgctgctc gctttcattt tgatccagag attcttgcaa ttgctgccct
     2341 ggatgaaaaa caacactttg catgacataa tccatgaatt ttgacgtaac aaaatgactc
     2401 gccgctatga tttcatgcac aatgatagaa tcgccaagaa actgagttct cagcctgact
     2461 ttatccacat aaggataatt ttttggaggt tcaataggat aagaagtctc aatgacaggc
     2521 agattgttat tcataaatct gtccattctg agattagcag catactcaga caaatgtcgt
     2581 gaaacatatt ctttttcaag gaaagcctca acatcaagaa aattcagttt ctttttcggg
     2641 agtttgtgac cgtagatatt ataaaatatc atttcacgcc agatagggtc ataaaatctg
     2701 aaactcatac gctcaaattt atctgtctgg ataatatcaa catttgccgg gacataagga
     2761 gaaaccttat cgggaagaaa ggctgtaaac ataccggaag gggaggtatg cgcgggccat
     2821 tccttttgta tactcccgcc atttactttg tagccatctt tggcgtacaa ggagtcaaaa
     2881 aacttgcggg ctgcaaatga gttcattttg ccctcatccc ccatcacaat ttgttgaatt
     2941 ttacttatgc ccaagaaaaa cgtgcgcata cgaatatgct gtcgtcccat atttggatca
     3001 tcgtatgaaa ttttgatttc tccaccagga acgccgtatt gatacagaaa attctcacga
     3061 ctatgaacct tcccgttcat attgctataa taactcgcaa aaacatccag atcttctata
     3121 atgaaccttc gaatataact gtcatgaacc ggaactccca gactttgatc aaaacgcaca
     3181 atgtaatggg taacagaatc cttgtaaggt ctttggtccg aaactgcgac agcctgaccg
     3241 gattgcgcca ccgtttttga attgattcta aagtttagtg cggtttcttc tacgttatcg
     3301 agtatacgca cgtcaaaaag cgagccttcg gcgctataat tatgccaatt ttgcgcttgc
     3361 gcgaatggcg ataaaatacc gctgaatgtc aatgcaacca gaccaagcca gacaaagtgc
     3421 ctgaatcctg ggcggtatat acgacgacga gagagagaga atgaaatgtt catacgattc
     3481 agtataaagc ataaaaaaac cgctgaaaag aaaacagcgg attttttgga gttttaaatt
     3541 acaaaaaact gcttttagaa tccatcgcgt tcacggcgct tacgatccat cttgcgcgcg
     3601 cggcgcacag attcagattg ttcgcgggct ttcttttcag aaggcttttc gtaatgacga
     3661 cgaagcttca tctcacggaa aatgccttca cgttgcattt tcttcttgag aaccttcaaa
     3721 gcttgatcga cattgttgtc gcgtacacta acactgacca cagtgcatca ctcctttcaa
     3781 gagtgttaaa gttaaaattg tccctgtaca ccgccgaagc agcgcaaaag gcccacattc
     3841 ctaccatctc gtacaaggaa tgcaagggaa aaatgcctca agctttaatt atactagttt
     3901 ttctaagccg ttttcaaatc cagcgccgcc gccagcagag cttttgtgta gtcttcctgt
     3961 ggatttccga agatcgcttt cgctggtccg gcttctacca cgcggccctc tttcatgaca
     4021 atcaaatcat gcgccatggc tttgaccacg cgcaaatcat ggctgatgaa catataggaa
     4081 atattgtgcc tctcctgcaa ttcacgcagc aaatcgacaa tctgcgcctg cacagacaaa
     4141 tccagcgcgc ttgtcggctc atccatcacg ataaatttcg ggtgcaagga tagcgcacgg
     4201 gcaatcgcaa tacgttgcct ttgcccgccg gaaaattcat gcggatagcg atgtcgcgtc
     4261 tccgggtcca tatgcacatc ttccagggcc tgcaccacca gcgcatcgcg ttcttcacgg
     4321 cttaaagcgc tgcggtggat tttaagaccc tcgccaataa tctgctccac gctcattcgc
     4381 ggggacagcg agccgaacgg gtcctgaaac acgatctgca tctcttcacg caagggcagc
     4441 atctctttcg cgttatggct cgaaatatca cgcccctcaa acacaatccg cccctgcgcc
     4501 cccaaaagct taagcaaccc aagccccagt gttgtcttgc ccgagccgct ctcgccgacc
     4561 acgccgatag tttgcccttt tttagcaaca atgtcgatat tatcgactgc tttgacccag
     4621 tccagcggct tgccaaacag gtttttcgcc ttggggaaat aaatcttgat atccgagcct
     4681 tccaaaatca ctggcgcgtt catatctgcg gggattgcat cccccttcgg ctgggccgaa
     4741 agcagcattt tcgtatattc atgctgcggc ttggcaaaca gcttcgccgt ttcgttctgc
     4801 tcaacgattt tcccgcgctg cataacacac acatgatcgc tcattttctc cacaatcgtc
     4861 agatcatgcg tgatgagcag cagcgccatc cccagccgct tttgcaaatc tttcaaaagc
     4921 tccagcactt gcgcctgcac ggtgacatcc aacgccgttg ttggctcatc ggcgatcaat
     4981 agctccgggt cattcgccag cgccatggca atcatcaccc gctggcgctg tccgcccgat
     5041 agctcatgcg gataggcatt caaccgcgtt tttagcccct ccagtcccac cagttccagc
     5101 aattccaaaa cccgcgcccg cgcttgcggc ttattcatct tctggtgcaa aaacagcact
     5161 tccgaaatct gtttttcaat cgtgtgcagc ggattgagcg cggtcaaagg ctcctgaaaa
     5221 atcatcccga tcttccgccc gcgcaccgtg cgcataaagc tttcaccctt accgaccagt
     5281 tcctcgccgt taaactggat cgccccgcca tccgggtgca cagcgcgcgg gtagggcagc
     5341 aattgcatga tcgacagcgc cgtaaccgat tttcccgacc ctgattctcc gaccagagcc
     5401 atcgtctcac ccttctggat ttcaaacgac acatcatcga ccgcgcgcag gccgccgcct
     5461 tgcgtcttaa aatcaacgga aagatttttg atttggagga gtgtttcggt catgtcgccc
     5521 ttatctcttt agtcatctca tggaaatgag atgcccgctg ttttcttctt tcttcaatcc
     5581 catctctttt cttttccaaa aattttctgg cgttccaatc aggattaatt tcaaaatttt
     5641 catccggtat ttcgtatttt tttcgtaacg cctctttcag ctctatagca tctaggccaa
     5701 gattcgcata gccgtcctgt cttgcttcaa agaattcttc ataatcaggc tgtcccatga
     5761 tagtgccgtc atcgccgaca acttcgcagc attttccgac atattcatca aggacacata
     5821 ttatctgaac ggccagatac gcagcatgtt tttcgcgcag ccgtttttca ttcagccact
     5881 ctcttaccca agggataaat gacccgataa taaccccaag aaagccgaaa attgcttcgc
     5941 tcatgctgtt gattccttat tcatcggcgt ttatctgtgt caatctgtgg ttatttaacc
     6001 gacagaacag aataaatctc atccgtgatc cggccttcca cagcctgcat atcggccaga
     6061 tcaagctcat ccagcttaca gcccttctcc tcggcgagct tcacaatcgc ccccgtcaca
     6121 tggtgcgcat cacgaaacgc catgcccagc tccatcacca gccagtcagc cagcgccgta
     6181 gccgtcgtaa acccatcctc ggcagctttg agcatatttt cggtgttaaa cgtagcggtt
     6241 gagagcatcc cctccatcgc ctcaatacac agcatgaccg tatcaaagct ctcgaacacc
     6301 ggtgccttat cttcctgcaa atccttgtta taggccaacg gcaggccctt taaaacggtc
     6361 agcatctgaa tcaaattccc gttcaaacgc ccggtttttc cgcgcaccag ctcggccgca
     6421 tcaggatttt tcttctgcgg cataatgcta ctgcctgtgc tccactgatc cgaaagctgg
     6481 ataaacccga attgcggcgt cgcccacaaa atgatttcct cagccagccg cgacaggtga
     6541 atgccggtct gcgcacagca aaacaaaaac tcggtcgcaa aatcccgcgc gcttaccgca
     6601 tcaagcgtat tgagcgtcgg tcggtcaaaa cctagctttt gagaggtaaa ttcccggtcg
     6661 atagggtagg gcgttccagc cagagccgcc gagcccagcg ggcatttatt cgcccggctt
     6721 tgacaatcac gaaaccgaag cttgtcgcgg ttgagcatgc aggcataggc atccagatgc
     6781 agccccagcg taatcggctg cgcggcttgt aaatgcgtaa agcccggcat aatgctgttt
     6841 ttgtgcgtgt cacttaaatc accaagagta tcttgaaaag cctcaatttt agcgacaatg
     6901 tcgttaatag catcacgaat ccacattctg aaatctgtgg ccacctgatc attccggctg
     6961 cgcgcggtgt gtagcttacc cgccgcctca ccaataagct cgcgcaaacg gctttcgata
     7021 ttcatatgaa tgtcttccag ctcgcgcttg aattcaaatt ccccggtctc aatctccccg
     7081 gcaatctggt ccagcccctt caaaattgcc tcaccgtcct tggccggaat gatcttctgc
     7141 ttcaccagca tctcgcaatg cgccttcgat ccggcaatat cctgccgcca caggcgtaca
     7201 tccacatcaa tgctcgcatt gatctgctcc ataatgtcgg acggcttttc gctaaaccgc
     7261 cccccccaca ttttattggc tttatttttg ctcattctct tttttaaatt cctgccttaa
     7321 catttcatat aggccgacaa taaaagtgca tgcgaccatc caaaaccata tagagatccc
     7381 taaagtatag aataaataag aagagaaata taaaggtata aaaagcactg ctgcaacaaa
     7441 gcctgttggt atggccataa ttatcttctt aattaggttt ttcatatttt ttttaagtca
     7501 tccccgcctt gtgcggggat ccggatgccc gcataaagcg ggcatgacat ggttgtgcta
     7561 gctacaaata tcttcgtata aatctttcca gtctgggttc atctcttcaa tgattttcat
     7621 tttccattct ctgggccatt ttttcaggcg cttctcacgg cggatggctg tctcaggatc
     7681 atcatgaacc tcataataaa caagcgtttt gataccgtac ttttttgtaa agccgtctgc
     7741 aacctcgttt ttatgttccc atatgcgcct tggtaaatcc gaggttatgc cgacataaaa
     7801 cgtactgttg cgggctttgg acagaatgta aacgtaatat tgttttggca tttacatctc
     7861 cggatgcccg cacggaagcg ggcatgacgg cgcagaggtt atgccgccgc cttgctcggt
     7921 ttactcgcag gcgtctcccc gcgtaccttg gtgttattcc gcaaccgcac ggcattaatc
     7981 ttgataaagc cttcagcgtc tttctggtca tacccgcctt cttcatccat cgaggattgc
     8041 gccgggtcat acagtgcatt cggcgatgta cgcgccaccg gataggcgct gcccttgaac
     8101 aactgcacct gcaccgtccc gttcacggtt tcttgcgcct tgtccatcgt cgtcatcagg
     8161 agttcaaatt ccggcgagaa ccaaaacccg ttataaatca attccgctac ttttagcgac
     8221 aatgtatcgc gcagcttcat tacctcacgg tccatggcca gcccttcaag gtcgcggtgc
     8281 gcattatgca ggatcgttcc gcccggcgtc tcataaatcc cacgtgattt aatcccgaca
     8341 aagcggtttt caaccatatc cagacgcccg atcccgtgct tggcccccaa ttcattgagg
     8401 aactgaaaca gcgccaaagc gcccgtaacc gttttgccct cggcctcaac cttgactggc
     8461 accccggcct tgaactcgat cgaaatcagc tccggctcat ccggccagtc tttaatcgtc
     8521 gatgtacgcg aatacacatc ctcggcgcag ggcgcattcg ggtcttccaa aatgcccgcc
     8581 tcatgagaaa tatgcagcag attatcatcc tcggaatagg gcttcttgcg cgtcgcgctc
     8641 accggaatgc cgtactggtc acagtaattg agcatatcct ggcgtccttc aaaccgggtc
     8701 agaaactcgg gcatcttcca cggcgccagc actttaatat cgggcttgag cgcataataa
     8761 gacagctcaa aacgcacctg atcgttgcct ttaccggtcg cgccgtgacc gacaaactgc
     8821 gcgccttctt tttcggcaat ctcaatctgg cgcttggcga tgatggggcg cgccacagag
     8881 gtgccgagca aatagcgccc ctcgtaaacc gcttgcgatt taaacgccgg gaagatgtag
     8941 tcggtaacga attcttcctt gaggtcctcg acataaacct tcgccgcgcc gatcttcagc
     9001 gccttttccc gcgccgcttc gaaatcctct tcctgaccga catcggcgat ataggcgata
     9061 acctcatagc cttcttcgat aaaccatttc aaaatcaccg acgtatccag gccgcccgaa
     9121 tacgccagta cgattttacc tttgctcact tgttaccttc cttttcgttt ttgaaccacg
     9181 aataaacgcg aataaataca aaattaaaaa tatataacta cagagcacgc gagagaaggc
     9241 aaggcctcaa agactccgaa ctacaataat tttatagaat ttatatctat cttcctaaaa
     9301 aaactacttt taaaatttag acagacagaa acacaagaaa agcgtgaatc attctagccg
     9361 gcagacaatg tgggcacagg ctgatcattt gatgaacatt ctggatttgt tttattatca
     9421 agggatgagc ccacgtaaat catgcccgcc aagcttgccg cagtagtaaa atcaagaact
     9481 cttctgacga caaatttttt atagttgggg actttaccgg cttcgccgca atcatcaaca
     9541 ggcactttcg tgaagtaatt ttttgcactt gttacgctac ttgcggcgat ttcttgacat
     9601 ccctgaaaga aagcacttac cccttcttta tctccatctt gacgattggt atcatctaaa
     9661 taacgagtca tttcctatcc tctttatagt tgtattgttt cgtaaaatat atcttttaaa
     9721 acaaaaaagc gacacgtcaa catgttttac ggaaatatat tatgttttaa aagtaagcaa
     9781 gctgtttctc ttgcgcactc tgcgatctct gtagttacat tctattcgtg tctatctgtg
     9841 tccattcgtg attatcaatc acgcattccc gaaattaaaa ctgctctggc tgacgctcac
     9901 cccggcctct tcaaggcacc aggccagaat ggctttttgc gcatgcaggc ggttttccgc
     9961 ttcgtcgtaa atcacattcg ccggactgtc cagcacgcct tgggccactt cttcgtcctt
    10021 gtgcaccggc atgcagtgca tgaagatcgc gctgtcttgc gcttttgcca tcaaggccgg
    10081 ggtgacctga taatggcgga acttgtcaat cgccttgcct tcctgaccca tcgaaaccca
    10141 tgtgtcagtg accagaacat cggcatccct ggccgccgcc tccggatctt cgaaataggc
    10201 cacataatcg gtgagatcat caggcttcag ctccagcggc accgacagcg ccagctcaaa
    10261 accgaaaatc ggcgcggcct gcacgaatgt gttgctgaca ttgttgcaat cgccaaacca
    10321 cgcaatcttg cgcccggcta tgctgcccag cttttcttcg atggtcagca tatcggccat
    10381 gatttgacac ggatgcgtct tatccgtcat gccgttaatc accggaatgg agcaatgccc
    10441 ggcgaagtct ttcagaacat catcatcgct catccggatc atgaccccgt caacatagcg
    10501 cgacaccaca ttggccgtat ccttgacgct
//
LOCUS       DAOSWR010000071        10396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4058_length_10396_cov_5.491925, whole genome shotgun sequence.
ACCESSION   DAOSWR010000071 DAOSWR010000000
VERSION     DAOSWR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_10396_cov_5.491925"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK513_06430"
     CDS             <1..201
                     /locus_tag="PK513_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82119.1"
                     /translation="GTYNVRVDALDINDEAVGSTIVVEGRVRGVETQNGLVFLLIGER
                     AVSVSNILNVSQPNSEDTTRNT"
     gene            217..351
                     /locus_tag="PK513_06435"
     CDS             217..351
                     /locus_tag="PK513_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82120.1"
                     /translation="MNTLAQTTPLKKPSLLVLTLCLSKAAVIAMTHRLQMACVFYLKP
                     "
     gene            409..2157
                     /locus_tag="PK513_06440"
     CDS             409..2157
                     /locus_tag="PK513_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOO82121.1"
                     /translation="MGLFGSLFTGVSALFAQSQNTAIISNNIANISTTGFKRSEAAFE
                     SLVTSQSRLSTYSPGTVTANRLQRVDQQGALQQTASTTDAAISGNGFFPIKRDPSDQQ
                     EFLYTRAGQFSEDSQGLLRNAAGFALYAWPIDANGDLPANQGDLTSLVPADVAFLGGL
                     TRPTSNAELAINLDANAIDKSLAASVTGAADFSRGITVFDSLGSAQVLTLEYTKTYGP
                     QATAGGSIGSLLATDNLITDLGLTPGDTFDVSTELGGPLTMTVVAGTPALPGDVQTVN
                     DIITEINNAGIGASAFLGNDGELVIQRDEFTGGAVNTLTIANGVGTPLTGLGLIAGVY
                     TSDDLSGAAPNYDNGLFADSPPYSNSGGTDTNTFPTFQFLPGDAQYNNRGWWQLNIKH
                     PDGTSLSRGLLNFNGDGTLNSLPDNSGDIDINLTQIDWNNGSNAQNIDIDIERFSQFA
                     GNFDVIFSDQNGAELGLRTGVEIDRDGVVIARFSNGASADLYKIPLITFANSNGLTEV
                     SGTAYSENQESGEENLREAGTGGAGFVEPSTLEASNVDLADEFARLIVSQRAFGAGTR
                     VINTVDQMTEDLLRLR"
     gene            2482..4074
                     /locus_tag="PK513_06445"
     CDS             2482..4074
                     /locus_tag="PK513_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82122.1"
                     /translation="MTVGEIILGSALKGTLLSINRTQSTIDEITQRLASGLKVNSAID
                     QPQNFFTARSLKFTASDQTRLLDGIGQSIRTIQEAITGLEAVERLINQGEALVIESEK
                     ALLLGETDPAVVPRIVNVSPPPLSTLITAGAPDAYFRLNETAGAIQDYGTLGPVTANR
                     LGGASAGAAALYSNGAAPSVNFDGINDRILVADAPHINLNATPARTVELVFNADDLTG
                     RQVLYEEGATINGLTIYLDGDRLYVTGEDDQGAQRWADANINSSAIGVQIQTGQTYHA
                     AFVYDMATNSFSGYLDGVLMGSVSTAGAPSFPSHSGNIGIGAAVDGVQFHDGEAGAGF
                     NFDGRISDVAIYNRALSTTELLRHADSLEGTTSTIYENINYNNLLDEIDNLVVDAHYR
                     GINLLLGEDLQTIFNAEQTSTLTTEGQDFSTRGLKLVKRSQFNNLTDVRDILDALRDA
                     RAVIRAFAFTLTNDLSIIEIRNTFTRDTINANLAGADDLTVADMNKEGANQLAAQTRL
                     DLGITALSLAGLSQGSILDLVG"
     gene            4258..5496
                     /locus_tag="PK513_06450"
     CDS             4258..5496
                     /locus_tag="PK513_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82123.1"
                     /translation="MSSDVVLTAALRNNLLSLQNTQSLIDSTQLRLATGLKVNSALDN
                     PQNFFTAQSLNNRAGDLGRLLDGIGQSIRTIEAADNGITALTKLVEQAQSIVQSARDE
                     LAASEGEARLVGTVDLRDTGTGDLVADLGIANGATLDIITTDDSGAQITQQVTIDTGD
                     TAYSLAAEITNAFADNENGEIVASISDEGFLQIESADGRSFRLVDEATTAITQAGLDA
                     LGIGEAFSTETRTGAGTTDLTATIVAGSTVTSISLFEGTGDLVEAGDLIGGAGVSYTD
                     ADGNTAFSLTTGSLGITVTETDGTSTSSTVVAVTGSFQDFVDSINQDANLNQLVSANF
                     DSSTGQLSITSLDDQVANVEINVAGATAADTISLGLGDPTGNLDPITAEAGAEDISIR
                     FNNSTEALDSLAGDYNTLRA"
     gene            5557..5940
                     /locus_tag="PK513_06455"
     CDS             5557..5940
                     /locus_tag="PK513_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO82124.1"
                     /translation="MVTFFNENNTSSLTTSGVTFTANGLSITEASFRSSTEIELSATQ
                     ARNALEAVRSFGSSLANNLSIIQTRQTFTEQTINTLQAGADDLVVADQNEEGANLLAL
                     QTRQALGVTSLSLASQSQQSVLRLF"
     gene            6106..6507
                     /locus_tag="PK513_06460"
     CDS             6106..6507
                     /locus_tag="PK513_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HOO82125.1"
                     /translation="MALVIDLKPGEKILIGEAVITNDSQRTRLHIAGDAPILREKDVM
                     QERETDTPCKKIYFLVQCMYLANTPKRYYEKYFALVQEVQHASPSSAFFFMQINEEII
                     SGNYYKALKLARQLIAHEKELLENASQPAAK"
     gene            6482..6916
                     /locus_tag="PK513_06465"
     CDS             6482..6916
                     /locus_tag="PK513_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HOO82126.1"
                     /translation="MPPNPPQNNPYAQAAGAYGDNAQKNTPDQRELEGRVLLKSAKMI
                     KDLQDDWENMNTEILESTLKYNRQVWMVFYDTAIENPEGDRPNDLRSNIVNLANFIFK
                     RELEIMAKPEKHKLDVLININREIAAGLMVKPPETTHPKDKT"
     gene            complement(7001..7510)
                     /locus_tag="PK513_06470"
     CDS             complement(7001..7510)
                     /locus_tag="PK513_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82127.1"
                     /translation="MIEAVNSAISNAQQARFTADQVATLNSFAADIEAVESVARGPVA
                     PYVSPYVSVDTRFDTAVLQIRDSDTGDILTQFPSESTLEQRRVQAARQAAARAEIERT
                     TADPVNTARSSTSSSNFDVTSAAFQTQQVAQSTPTQSVGGPAQAAVAALSAGAQSGQQ
                     FASTVSVTA"
     gene            7907..9553
                     /gene="fliF"
                     /locus_tag="PK513_06475"
     CDS             7907..9553
                     /gene="fliF"
                     /locus_tag="PK513_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOO82128.1"
                     /translation="MSSFVDTLKQLGPSRLAIMGGILVGLLLFFVFVSMRVSTPEMKL
                     LYSDLSSADSGAIAAKLEESQVRYEVSPDGGRIMVSEDEVGRARLLLAEAGLPNGGSL
                     GYEIFDKQSGFGTTNFVQNINQVRALEGELARTISSLSSVRSARVHLVLPQRELFSRE
                     SRPSSASVYLGVRPGAIVEREQILSIQSLIASAVPDLKADNVTIIDSNGNLLAKGGED
                     NRENMLSVKAEEMRRNYESRLTEKIEDQVSRIVGYGNVRAIVTAEVNFDRISTNEEVF
                     DPEGQVVRSSQVTEENNTERDPAPEDISVQNNLPGVGGDLLSDTAPTAENSRLEEVTN
                     FEISRTVRNTVREVGEVNRLSIGVLVDGRYITNEEGERVYEERSEEELAEIEELVRSA
                     AGYNEDRGDVITVKNLQFAEIDTNEEAIDESMLFGFQKSDLIDAAEVVLVGIMIILVI
                     LLILQPMVSRLLAVSDTELDEDLEADLLTMRPAAPALEGPDAIGAFGGGQGDEEESLI
                     DIQGVEGKVKSSSIRKVEEIVENYPNETVSVIRSWMTQES"
     gene            9609..>10396
                     /locus_tag="PK513_06480"
     CDS             9609..>10396
                     /locus_tag="PK513_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOO82129.1"
                     /translation="MRVREDYRTLSGPEKAAILLLSLPEDQTAKIFEQMDDEEIMELS
                     QTMAGLGKVSPNVVERLFVDFAEQMTSTNSLIGTQDSTERLLAKAGLSGDRIENIMEE
                     IRGPAGRTMWEKLGNVNEEILATFLQKEYPQTVSVVLSKIQTDHAARVLGMLPENFAL
                     EVIHRMLRMEVVQKDILEEVERTLRQEFMTNLARTQKKDSHELIADIFNNMERAVETR
                     FMEELEKSAPDSAEKIRSLMFTFEDLINLDPASVQTLIRAVDKD"
BASE COUNT         2753 a         2501 c         2601 g         2541 t
ORIGIN      
        1 ggaacataca atgtgcgggt tgatgccctg gacatcaatg atgaagcggt tggatcgaca
       61 atcgttgttg agggccgcgt acgcggggtc gaaacacaaa acggccttgt cttccttctg
      121 attggagagc gcgccgtttc cgtcagcaat attttaaatg tcagccaacc gaatagtgaa
      181 gatacaacca gaaacacctg aagaggagca aacagcatga acaccctagc ccaaacaaca
      241 ccgttgaaga aaccttcatt gctcgtttta acattatgcc tgagcaaggc ggcagtaata
      301 gctatgacac accgcctgca aatggcctgt gtgttttatt taaaacctta accccaacac
      361 tcaaaaccac aagagacgca aacaagattt agacaaagga gaaaaacaat gggacttttt
      421 ggatcactct ttaccggcgt atcggcgctg ttcgcacaaa gccaaaacac ggcaattatt
      481 tcgaacaata ttgccaatat cagcacaacc gggtttaaac gttcagaagc ggcatttgaa
      541 agccttgtaa catcacaaag ccggttatcg acttattcac cggggacagt gacagccaat
      601 cgcctccagc gggtcgacca acaaggcgcc ctacagcaga ccgcttcaac gacggatgcg
      661 gcgatttccg gtaacggttt cttcccaatc aaacgcgacc cttccgacca acaggaattc
      721 ctctacacgc gcgccgggca attttccgaa gattcccaag gacttttgcg gaatgccgcc
      781 ggatttgcac tttatgcgtg gccaattgat gccaacggcg accttccagc gaaccagggc
      841 gaccttactt ctttggtgcc ggctgatgtg gccttccttg gtggattgac ccgcccaaca
      901 tcaaatgcgg aactggcgat taacctggat gcaaatgcga ttgataaatc cctagctgcc
      961 tctgttactg gcgcagcaga tttttctcgt ggaatcaccg tgtttgacag cctgggttca
     1021 gcgcaggttc taacgctgga atacaccaaa acatacggac ctcaagctac tgcaggcggc
     1081 tccatcggca gcttgctcgc gactgataac ctgatcacag accttggttt gacaccaggc
     1141 gatacgttcg atgtttcaac cgaacttggc ggaccgttga ccatgactgt tgtagccgga
     1201 acgcctgccc tgcctggcga tgtgcagacc gttaacgaca tcatcaccga aattaacaat
     1261 gccggtattg gcgccagcgc ctttctcggc aacgatggtg agttggtcat tcagcgtgat
     1321 gaatttacag gcggtgctgt taacaccctt acaattgcca atggtgtcgg tacgccgctt
     1381 actggtcttg gactgattgc cggggtgtat acctctgatg atttgtctgg ggcagcgccc
     1441 aattacgaca acggtttatt tgccgattcc ccgccctatt ccaacagcgg cggcacagat
     1501 acaaacactt tcccgacctt ccagttcctg cccggcgatg ctcaatataa taatcgcggc
     1561 tggtggcagc tcaatatcaa gcacccggac ggcacgtcac tgtcacgcgg cctgctgaac
     1621 tttaacggcg acgggacgct caactccctg cccgataatt ccggggacat tgacattaac
     1681 ctgacccaaa ttgactggaa caatggatca aacgcacaaa atatcgatat tgatattgag
     1741 cgattctctc aatttgccgg gaactttgat gtaattttct ctgatcagaa cggtgccgag
     1801 ctcggtctgc ggaccggggt tgagattgat cgcgacgggg ttgtaattgc ccgattctcc
     1861 aacggcgcat cagctgatct ttataaaatt ccactgatca cttttgccaa ctctaacggc
     1921 ctgacagaag tctctggaac ggcttattca gaaaatcagg aatccggtga agagaatttg
     1981 cgcgaagccg gaaccggcgg tgccgggttt gttgaaccct cgacactgga agcgtcaaac
     2041 gttgatttag cggatgaatt tgcgcggcta attgtctcac aaagagcgtt tggcgcgggg
     2101 acgcgtgtaa tcaacacggt cgaccagatg acggaagatt tgttgagact gcggtaataa
     2161 tacataaagt ttggatcttg agctgtaggt tcgctacccg caaccgtcct aacccctaag
     2221 gctcagggtc cgaatatctg ctgactgcta tgatccagaa tggagagtag cgacttgcag
     2281 acgggcgcac cgggtaatgc cctaacctta cccatctggg agaacccaac cgctccggtg
     2341 cgccctcttt ttccacaaat actaatatta agtcctgttt ttttccaaaa cctcaagaca
     2401 ttgttaaaat ctttatgatt aaatgtaggg tgtagaattg cgtgtagtat atgcgcatgt
     2461 atgtaatttg caggatgcaa aatgactgtt ggcgagatca tcttgggatc ggctcttaag
     2521 gggacgttac tgagcattaa ccgtacacaa agtacgattg atgagattac acaacgtttg
     2581 gcttccggcc taaaagttaa ttccgctatt gaccagccac aaaatttctt taccgcgcgc
     2641 agcttgaaat tcaccgccag cgatcagaca cgcttgctgg acggcatagg tcagagcatt
     2701 cgaaccatac aagaggccat taccggtctg gaggcagttg aacggcttat caatcagggc
     2761 gaagcgctgg ttatcgaatc agaaaaagcg cttttacttg gtgaaactga cccagctgtt
     2821 gttccacgga ttgttaatgt cagcccacct ccgctcagca ccctgatcac cgcaggtgcg
     2881 ccggatgcat attttcgcct gaatgaaacg gcaggcgcta ttcaagatta cggaacactc
     2941 ggcccagtca ccgccaaccg attaggcggg gcaagcgccg gagcggccgc tctttattcc
     3001 aacggggcag ctcccagcgt caattttgac ggcatcaatg accgcattct ggttgccgat
     3061 gccccgcata ttaacctgaa cgcaacacca gcgcgtacag tcgagcttgt cttcaatgct
     3121 gatgacctca cagggcgaca agtactgtat gaagagggcg ctaccattaa cggtcttacg
     3181 atttaccttg acggtgacag gctttatgtc acaggggaag acgatcaggg cgcacaacgc
     3241 tgggctgatg caaacatcaa cagcagcgcc attggcgtac agattcagac cggacaaaca
     3301 taccatgccg cctttgttta tgatatggca acaaacagct ttagcggcta ccttgacggc
     3361 gtccttatgg gtagcgtcag caccgccggg gcgccaagct ttccatctca tagcggaaat
     3421 ataggcattg gcgcagccgt ggacggtgtg cagttccatg acggcgaagc tggcgcagga
     3481 tttaactttg acggacgcat ttctgatgtc gccatctata atcgcgccct tagcactacc
     3541 gagctattac gccatgccga ttcacttgaa ggaacgacat ccactatcta tgaaaatatt
     3601 aattataaca acctgttgga cgagattgat aatctggttg ttgacgccca ttatagaggc
     3661 attaatttgc tgctggggga agatttacaa accattttta atgcagagca aaccagcacc
     3721 cttaccacag aaggtcaaga cttcagtaca cgggggttga agctagttaa acgctcccaa
     3781 tttaacaacc tcacagatgt acgcgatatt cttgatgcgc tgcgcgatgc aagagcggtt
     3841 atacgggcgt ttgcctttac attgacaaat gacctatcta taattgaaat acgaaatact
     3901 ttcacacgtg atacgatcaa cgcaaatctg gccggcgccg atgatctgac cgtggctgat
     3961 atgaacaaag aaggagccaa ccaattggct gcacaaaccc gactggatct gggcattaca
     4021 gcccttagcc tagccgggct gtcgcagggc tccatactgg atcttgtagg atgaaaaatc
     4081 cttttgaaat gtggattgca cgaagcatgc gatgcaatat atccacagag gaatgcataa
     4141 aaagaaaggg ataaattgcg ttaacaatct tttcattttt atgatatatt ctgtaagagt
     4201 gagcccaagt attgggcgat gcaggaagca tgcacaaacc taggaaagga aaacactatg
     4261 tcttctgatg ttgtattgac cgcagccttg cggaataacc tactttctct ccagaacaca
     4321 caaagcttga ttgactccac gcagcttaga ttggctacgg gtctgaaagt taactctgct
     4381 ctggataacc ctcaaaactt ctttaccgcg caaagcctga ataaccgggc cggcgacctt
     4441 ggtcgtctct tggatggtat cggccaatcc attcggacta tcgaagctgc tgataacggt
     4501 attaccgctt tgacaaagct tgtagagcaa gcccaatcga tcgtgcagtc tgcacgtgat
     4561 gagttggcgg cgtctgaagg tgaagcgcgc cttgtcggta cagttgacct gagagataca
     4621 gggactggcg accttgttgc cgacctcggt atcgcgaacg gcgctacgct ggacattatc
     4681 acaactgatg attccggcgc acagatcaca cagcaagtga caatcgacac tggcgatacg
     4741 gcctattctt tggctgcaga aatcacgaac gcttttgccg ataatgaaaa cggagaaatc
     4801 gttgccagca tttccgatga aggtttcctt cagattgaaa gcgctgacgg ccggagtttc
     4861 cgtcttgttg atgaagcgac aacagcgatt acccaggctg gacttgatgc tcttggtatt
     4921 ggcgaagcct tctcgactga aacgcgcaca ggtgcaggta cgactgatct tacggccact
     4981 atcgtagccg gaagcacagt gacctcaatc tccctgttcg aaggtaccgg tgacttggtt
     5041 gaagccggcg accttattgg tggtgccggg gtgtcttata cagatgcaga cggtaatacc
     5101 gcgttctctc tgacaaccgg tagcttaggc attacggtta ctgaaactga cggcacatca
     5161 acaagctcaa ctgttgttgc tgttactggc tccttccagg attttgtcga cagtatcaac
     5221 caagatgcaa accttaacca gttggtgagc gctaatttcg acagctccac gggacagctc
     5281 agcattactt cgcttgatga tcaggttgcc aatgttgaaa ttaatgttgc tggcgctact
     5341 gctgctgata cgattagcct tggtcttggc gacccaaccg gaaatctgga cccgattact
     5401 gccgaggcag gtgctgagga tatttctatt agatttaaca acagtaccga agctctcgac
     5461 agtttggctg gggattacaa tacgctccgc gcctagatcg actctctggt tgccgatgct
     5521 cagtatcgtg gaatcaatct gcttaacggt gatgacttgg ttacattctt taacgagaac
     5581 aatacaagtt cactgaccac gagcggcgta actttcacgg ctaacggcct cagcattacc
     5641 gaagcctctt tccgctccag tacagagatt gagctgtctg ccacgcaggc tcgcaacgct
     5701 ctggaggctg tgcggagctt cggctcctct ctggccaaca acctgtctat tattcagaca
     5761 cgtcagactt tcaccgagca aacgatcaat acgttgcaag ccggtgcgga tgacctggtt
     5821 gttgcagacc agaacgaaga gggcgcaaac ttgctggccc tgcagacacg tcaggctttg
     5881 ggtgttacat ccctgtctct ggcgtctcag tcacagcagt ctgtacttcg actcttctaa
     5941 ttttaaattc aagggcattg cccgaattcg aaaaagccct agcagaacgc cggggctttt
     6001 tttatctcct gcagcgttta gattttttat attttataaa gataaaagct gatataatgg
     6061 cggagtatgg gcagaaagcc gtatctaaac gaaaggaaga attccatggc tctcgttatc
     6121 gatctcaaac ccggcgaaaa aatccttatt ggcgaagctg tcattaccaa tgacagtcag
     6181 cgcacacgcc tgcatatagc cggtgatgct ccgattctac gtgaaaaaga tgtgatgcaa
     6241 gaaagggaga ctgatacgcc ttgcaagaaa atttacttcc ttgtccagtg catgtatctg
     6301 gccaatacgc ccaaacggta ttacgaaaaa tactttgcgc ttgtacaaga ggttcagcat
     6361 gcttctccat cttcggcatt tttctttatg cagattaacg aggaaattat tagcggcaat
     6421 tattacaaag ccctgaaact cgcaagacaa ctcatcgctc acgaaaagga actcttagag
     6481 aatgcctccc aacccgccgc aaaataaccc atatgctcaa gctgctggcg cctatggcga
     6541 caacgcgcaa aagaacacgc ccgaccagcg tgagcttgaa gggcgcgttt tattgaaatc
     6601 cgccaaaatg attaaagacc ttcaggatga ctgggagaat atgaacaccg aaattctcga
     6661 gagcaccttg aaatataacc gtcaggtctg gatggtgttt tacgataccg ccatcgagaa
     6721 cccggaaggt gaccgcccca acgatttgcg cagcaatatt gtcaatctcg ccaattttat
     6781 ttttaagcgc gaactggaga tcatggcgaa gccagagaag cataagctag atgttcttat
     6841 taatatcaat cgtgagattg ccgccggtct aatggttaaa ccgcctgaga cgacgcaccc
     6901 caaagataaa acataatttc ttaggggctg acattaaaaa agcgcttccc ttgaagcgct
     6961 tgctcgataa tttaccatgt ataatttacc atgtataaac ttaggccgta acactcaccg
     7021 tagaggcaaa ctgttggccg gattgtgcgc ctgcgcttaa agcagcgaca gcggcctgtg
     7081 cagggccacc aacggattga gttggcgttg attgtgcaac ctgctgggtc tggaacgccg
     7141 cagatgtaac gtcaaaattt gacgaagacg tcgaagatcg agcagtatta acgggatcgg
     7201 ccgtcgtgcg ctcaatttca gcacgcgcag cggcctgacg tgcagcctga acgcggcgct
     7261 gttccaatgt tgattctgag gggaattgtg tcaaaatatc gcccgtatcg gagtcgcgta
     7321 tttgcaatac ggctgtgtcg aagcgggtat cgactgaaac atacgggcta acatagggtg
     7381 caacaggacc acgcgcaacg ctctccacag cctctatatc ggccgcgaaa gaattcaaag
     7441 tcgccacctg gtccgcagta aaacgggcct gctgtgcgtt cgatatggct gaatttacag
     7501 cttctatcat aattaacgtc ctaaccgcct gtccgattta taaaaaatat atgggaacag
     7561 gcataatacc ctactatcat ggtaccttaa ccgcaaacaa aagtcaagtt ttccgtcggg
     7621 tttcccacac catttcatgc ttaccaaccc cttgaaaaat aattaaaata ttttgatccc
     7681 cctccaaaat caagagaaga tgattttcag ctctatactc cccccttttg gaaggttttt
     7741 ctggaacaac agaccaatgg aaaaaggtta aaaaaacgtc tatactgtaa ggataacctg
     7801 ttttcccgat tcgctaaatc ggagaagata gtgaataaaa aaggctaccc ctatcctctg
     7861 tggggttcta gccatgcgca aaatgagaga ataaaggtcg gaaaccgtga gtagttttgt
     7921 tgatacattg aaacagcttg gcccgtcgag attggctatt atgggcggca ttctcgtcgg
     7981 attgctgctg tttttcgtgt ttgtttccat gcgcgtttcc acgccggaaa tgaaactgct
     8041 ctacagcgat ttatcatccg cggactctgg tgctatcgct gccaaactcg aagagagcca
     8101 ggttcgttat gaagtttccc cggatggcgg gcggatcatg gtttcggaag atgaggttgg
     8161 acgtgcacgg ctgttactgg ccgaggccgg acttcctaat ggcggttcat tgggatatga
     8221 aatttttgac aaacaatcgg gcttcgggac aacgaacttt gtccaaaata tcaatcaggt
     8281 gcgtgctctt gagggagaac ttgcccgcac aatcagctct ttaagttctg ttcgaagcgc
     8341 ccgcgtccat ctggttttac cccagcgcga actttttagc cgcgaaagcc gtccatcgag
     8401 cgccagtgtt tatttgggcg tccgccccgg tgcgattgtt gagcgtgagc agatattgtc
     8461 cattcagtcg ctgattgcct cggcggtacc ggatctgaag gccgataatg ttactattat
     8521 tgacagtaac ggaaacttgc tggccaaggg tggggaagat aatcgggaaa acatgctttc
     8581 cgttaaagca gaggagatgc gccggaatta tgaatctcgc ctgacagaaa agattgagga
     8641 tcaggtgagc cgtattgtcg ggtatggcaa tgtgcgcgca attgtcactg cggaagttaa
     8701 ttttgatcgt atcagcacaa acgaggaagt ttttgaccca gagggtcagg ttgttcgctc
     8761 ttctcaggtt accgaggaga ataatacaga gcgcgatccg gctcctgaag atatcagtgt
     8821 tcaaaataac ctgccgggcg tgggcggaga tctgctttca gataccgcac caactgctga
     8881 gaatagccgc cttgaagagg ttacgaactt tgaaatcagc cgcacggtac gtaacactgt
     8941 gcgcgaggtg ggggaagtta atcgcctgtc tatcggcgtt ctggttgacg ggcgctatat
     9001 cacaaacgaa gaaggagaac gcgtttacga agagcgctct gaagaagagc ttgccgagat
     9061 cgaggaattg gtgcgcagtg cggccggtta taatgaagat cgcggcgatg tgattacagt
     9121 taaaaatttg cagtttgctg aaatcgatac caatgaagaa gcaattgacg agagcatgct
     9181 gtttggtttc cagaaatctg atttgattga tgctgcagag gtcgtgctgg tcgggattat
     9241 gatcattttg gtaattctgc tgatcctgca acctatggtt tcaagactgc tggctgtcag
     9301 tgatacggag ctggatgaag accttgaggc tgatttgctg actatgcgcc cggctgcccc
     9361 ggctctggaa gggccagatg ccattggcgc cttcggtgga ggtcaaggtg atgaggaaga
     9421 gagcctgatt gatattcaag gcgttgaggg taaggttaaa tcttcctcta tccgcaaggt
     9481 cgaagaaatc gttgagaatt atccgaatga aaccgtttcc gttatccgca gctggatgac
     9541 acaggaaagt taaagcatga gagataccgc ccaacagcct atgaacagat gggagacgaa
     9601 ccattaatat gcgtgtgcgt gaagattaca gaacgttgag cggcccggaa aaggctgcga
     9661 tcctgttgct gtcattgcca gaagatcaga ctgcgaaaat ttttgagcag atggatgatg
     9721 aagaaattat ggaactctca cagaccatgg ccgggcttgg aaaagttagc ccgaatgtgg
     9781 tggaacggtt atttgttgat tttgcagagc agatgacttc aacgaactct ttgatcggga
     9841 cacaagacag tactgaaaga ttgctcgcca aggctggtct ttccggtgac cgtattgaaa
     9901 atatcatgga ggaaattcgt ggcccggccg gacgcacgat gtgggaaaag ctgggcaatg
     9961 tgaatgaaga aattctagcc acattcctgc aaaaagaata tccgcagacg gtttcggtgg
    10021 ttctttctaa aatccagact gatcacgccg cgcgtgtttt ggggatgctg cccgagaatt
    10081 ttgcgcttga ggttattcac cgaatgttgc gtatggaggt ggtacaaaaa gatattttgg
    10141 aagaggttga gcgcactttg cgtcaagagt tcatgaccaa cctggcgcgg acacagaaaa
    10201 aagattctca cgagcttatc gccgatattt tcaacaatat ggagcgtgcg gtcgaaacgc
    10261 gctttatgga agaactggaa aaatcagcac ccgattccgc agagaaaatt cgttcgctca
    10321 tgtttacatt tgaagatctt atcaatcttg atccggcctc tgtgcaaaca cttatacgcg
    10381 ctgttgataa agacaa
//
LOCUS       DAOSWR010000072        10131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4192_length_10131_cov_5.672588, whole genome shotgun sequence.
ACCESSION   DAOSWR010000072 DAOSWR010000000
VERSION     DAOSWR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_10131_cov_5.672588"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK513_06485"
     CDS             complement(<1..647)
                     /locus_tag="PK513_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOO82130.1"
                     /translation="MIADIFVLAVLLISGLIAFLRGFIREVLTIAGVVGGLAAAYLGG
                     PLLMPHMHGWFGIEEGAEVERLFEILPYGIIADALSYGAIFIIVVIILSIQSHLLAEW
                     AKKIGLGAIDRTLGFVFGVVRGIILLGLLYLPVHFFVDQERKEEFFSESKTHFYLEKT
                     SEILAGFIPSGAFEEVEEGVASLEKTSKTRKKLEEINLLKKDNGGGEQQQPAESP"
     gene            complement(720..1571)
                     /locus_tag="PK513_06490"
     CDS             complement(720..1571)
                     /locus_tag="PK513_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82131.1"
                     /translation="MLKSERQHLPVLIKADDLSFFMPGLTELAQSAYLQRQFDAAVSG
                     PKEYRIKLRDTITLSWDGGISRQRPSETMRVNYDCGYIYSSQEAKSEVSVGALGFSVN
                     MHFLDLQHIVIESCNFDDKAVGLVREHIDVSHDINADPVHEQLIYTLFFTALSRALPS
                     GTLPSSSEGSKHSKFLGLGLKAAADLIASKIESSGHHGYERTLYYPVRVGNSEDVQHY
                     NMLHPGVPDFVHNVVTHFALSQGHKGPDKIIRKSDIPLLDVQPSAPLQNHPSSAVLLK
                     YEGPETN"
     gene            complement(1641..3050)
                     /gene="radA"
                     /locus_tag="PK513_06495"
     CDS             complement(1641..3050)
                     /gene="radA"
                     /locus_tag="PK513_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOO82132.1"
                     /translation="MAKVKKSYVCQSCGAVSSRWAGKCESCGEWNSIAEETLAPSVPK
                     GMDGAKGRKIDFVSLSDPARSDYPRHVSDIQEFDRVVGGGLVPGSAVLIGGDPGIGKS
                     TLLLQLVCRLSQKNTRCVYVSGEESVDQVRMRAQRLELAEAPVQLASATSIRDILASV
                     EPVGDNGPVELLIIDSIQTMYVDNIDSAPGTVTQVRSSAQEIIRYAKTYGMAVMFVGH
                     VTKDGQIAGPRVLEHMVDTVLYFEGDRSHQFRILRGVKNRFGPTDEIGVFEMAGGGLQ
                     EVENPSEIFLSQRNQDVAGSAVLAALEGSRPMLVEVQALVAPSSLASPRRSVIGWDPN
                     RLAMIVAVLETRCGLQIGSCDIFLNIAGGIRINEPAADLAVAAALVSALQNNPLPPET
                     VFFGEVGLAGEIRQVAQPDLRLKEASKLGFREAVVPKGKKSQKPEKVYGDAIRVSEID
                     HVQMLGEIFGDGRAPRAVA"
     gene            3761..4951
                     /locus_tag="PK513_06500"
     CDS             3761..4951
                     /locus_tag="PK513_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO82133.1"
                     /translation="MTIAEHALTESIEFQMSLLLFMALGGYLLSNRIGQPAVVGVILA
                     GILIGPTGFNLIHYSDFVASIGHIGAIILLFVIGLEFHLKEITKPSYFLIALGGILLP
                     WAGGYAVALAFGFEFSKAIIIGVALSATSIAITADTLREMGKLKSEAAQAIIGAAIID
                     DVLALLALAIAQQLSDGGVDAAVVGVFTLKAIIFLVAGALFGDKVLTPLVHRIDRSKI
                     AEKYPDFIFILIMMVAFFYAMAAELMGLSAIVGAFVAGVSLEHVHTQRSKDFRVGSEY
                     LRIVFGAIFFISLGILADFKNFTWEAAWFALALTIVAVLTKLVGCGILARLSGMSVHD
                     SLVVGVGMSPRGEVAMTVALLAFISGVIEQPAFVGLILMSLLTTIIAPLLLRNWLYRN
                     KTEA"
     gene            4965..5963
                     /locus_tag="PK513_06505"
     CDS             4965..5963
                     /locus_tag="PK513_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOO82134.1"
                     /translation="MLTILAPNGGEETLKALAEETNIIEFWSGAKPKGKPREINVLVA
                     SEYLQDLVDKLQRRFSKEKEWRIIITPVETTIPRHEEKEEENANAKKAYGSLTREALY
                     DQILKGTKTNTDFIILVVLSTIVTAIGLVTDNLAVIIGAMVIAPLLGPNLALSFGVTL
                     GDRDMVAESLKANAMGFGLTMLLSILAGALIPYEVFADSREYLLRTNVGYDGIILAFA
                     SGAAAVLSLTAGISSTMVGVMVAVALMPPAVAMGLSLGEGAFAQAYGAALLLAINIIC
                     VNIAAKAVFTLKGIRPRTWYQRKKSRQSLKISLTFWGILLLLLIALIYFWHHGGTG"
     gene            complement(6002..6679)
                     /locus_tag="PK513_06510"
     CDS             complement(6002..6679)
                     /locus_tag="PK513_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOO82135.1"
                     /translation="MQKDPDCYLLKENKNSYPFDLWRSFKIWREFQKGFRALRCINHC
                     ITFFGSARFGEDDPYYKMARETAFELGKAGFSIMTGGGPGIMEAANRGAKDAGALSIG
                     CNIKLPHEQKPNPYTDINIQFDHFFVRKVMLLKYSCAFVLLPGGFGTMDEIFETATLI
                     QTGKICDFPIVAMGSEYWGALGPFLRNTMIEYGTIDEKDLDFVRITDHPKDVLEIIRA
                     RKTGVHL"
     gene            6756..8117
                     /locus_tag="PK513_06515"
     CDS             6756..8117
                     /locus_tag="PK513_06515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO82136.1"
                     /translation="MKLTFLGATGTVTGSKYLLEDDNKKILIDCGLFQGMKELRQRNW
                     ENLPVNPASINAVILTHAHIDHSGYLPLLVKNGFDGQIYCSSATADLCDILLPDSAHL
                     HEEDAERANRYGYTRHKPALPLYTQEDSYNALEKIEPVSFGDEHALSDILSFRLTHAG
                     HILGASCVHISDGQTNIVFSGDLGRPRDPVMKVPAQIQEADYLILESTYGDRLHETTD
                     PLDQLEDIIIRTSGRGGTVVIPAFAVGRAQSLMYYIYKLKEMKRIPGIPVYLDSPMAI
                     SATKLLQQHHAEHRLSKEECAKVCNSVIYTQTVEQSKAIYSKNNGMPTVIISASGMAT
                     GGRVLHHLKHYISDPRNTVLLAGFQAEGTRGARLAHGEVEIKIHGELLRVRAEIDMLD
                     NISAHADYQEIIDWLGHFREPPRRTFLTHGEPKAATSFKFKIKEHLDWNVEIPEYEQM
                     AEL"
     gene            complement(8191..>10131)
                     /locus_tag="PK513_06520"
     CDS             complement(8191..>10131)
                     /locus_tag="PK513_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOO82137.1"
                     /translation="GRSRGRGGRDNGRGRGGRGRNAAHRARRVETFGGEDMDDEQRFR
                     FNLRRKYKIQEVIKRGQIMLVQVSKEERGNKGAAVGSYLSLPGRYCVLMPNSPRAGGV
                     SRKISNYKDRAKMRDMMKDLEVPKGMSVILRTAGVTRTKVEVKRDLDYLLRLWNTIRE
                     KTLESSAPALVYEEADLIKRAVRDLYTRDIEEIHVAGDEGFKTAKEFMKMMIPSHVKK
                     VKEYKDVDTPLFNKFRVEEQIAEIGETEVRLKSGGYLVINPTEALVSIDVNSGKATKE
                     RHIEETALKTNLEAADEVARQLRLRDLGGLVVIDFIDMEDRRNNGKVERKMRDALSGD
                     RARIQMGRISSFGLMELSRQRLSASLSESQFETCPGCQGAGRIRTADAASIMVLRAIE
                     AEGIKGRASQVIVQVSNEVALYILNNKRDNLAVIEERYGFAVLIRVDDTLGASGFRVE
                     VSKPVDKTEDDSDDTPAGEDDQDGNNRRDGRRRGRRGGRNRGMRNQPRKDDNAGEEQA
                     DQKSQNAVVQADSKVDEKAPQEKKKPSASAEKATVDAASKKVRVKKEESTGKDDKKPS
                     ASAKTMADGATKKAPVKKAKKNDEPVEPKDEKPAKVVKVSNDDAKPKKVAVKARKDDA
                     PKDYETVNEAPKAKKKGWWNLK"
BASE COUNT         2618 a         2676 c         2253 g         2584 t
ORIGIN      
        1 tccggagact cggcaggttg ttgttgctcc ccgccaccat tgtctttttt taaaagatta
       61 atctcttcaa gcttcttgcg cgtctttgat gttttctcaa gcgaagcaac cccctcttca
      121 acctcctcaa aagcaccgga aggaataaaa ccagccaaaa tttccgaggt tttctccaaa
      181 taaaaatgcg ttttactctc gctaaaaaat tcctcttttc tctcctgatc aacaaagaaa
      241 tgcaccggca aatacaacaa accaagcaaa ataatcccgc gcacaacacc aaacacaaac
      301 cccaacgtgc gatcaatagc cccaagcccg atctttttgg cccattccgc caaaagatgg
      361 ctctgaatag acaaaataat caccacaata ataaagatcg ccccgtaaga cagcgcgtct
      421 gcaataatac cataaggcaa aatttcaaac agtcgctcaa cctcagcgcc ttcctcaatc
      481 ccaaaccagc catgcatatg cggcatcaat aacggcccgc cgagatatgc cgccgccaac
      541 ccgccgacaa caccggcaat agttaaaact tcacgaataa aaccacgaag aaaggcaatc
      601 aggcccgaaa tcaaaagaac ggcaagaaca aaaatatcag cgatcatgga aacgtcaggt
      661 cctttttgga tcagataagc ctcatattta ggcaaaacca tctcaaaaag caagccttct
      721 cagtttgttt cagggccttc atatttcaat aaaacagcag acgatggatg attctgaagc
      781 ggtgcggatg gttgaacatc caataaaggg atatcgctct tacgaataat tttatcagga
      841 cctttgtgcc cctgagagag ggcaaagtgc gtaacaacat tatgaacaaa atccggaaca
      901 ccaggatgaa gcatattata atgttgtaca tcctcagaat ttccaacacg aacaggataa
      961 tacaaagtgc gctcataacc atgatgaccg ctcgactcaa tctttgaggc aatcaaatcc
     1021 gccgctgctt tcaaccccag gcccaaaaat ttcgaatgct tagatccttc agatgaagat
     1081 ggtaaagtgc ccgacggcaa agcacgagac aaagcagtaa aaaatagcgt ataaatgagc
     1141 tgttcatgaa cagggtctgc attaatatca tgagaaacat ctatatgctc acgaaccaac
     1201 ccaacagcct tatcatcaaa attacaagat tcgataacaa tatgctgtaa atccagaaag
     1261 tgcatattca ccgaaaaccc gagcgcccct actgaaacct cgctcttagc ctcctgagaa
     1321 gaataaatat acccacaatc gtaattcact ctcatagttt cagaaggtct ttggcgcgat
     1381 attccgccat cccaagacaa agtaatcgta tctctcaatt ttatacgata ctcctttggc
     1441 ccactgacag cagcgtcaaa ctgtctttgc aaataagcgc tttgagccaa ttcagtaagc
     1501 cctggcataa aaaaagaaag atcgtctgcc ttgatcaaaa ctggtaaatg ttgcctctcg
     1561 gattttaaca tcctaaacct ccaaggcaga agaaatatag atgtttacgt aaaatgtaaa
     1621 gtttttttaa tagcctgagc ctatgccacc gcacgcgggg cccgcccatc gccaaaaatt
     1681 tcccccagca tttgcacatg atcaatttcg gaaacgcgga tggcatctcc ataaaccttt
     1741 tccggttttt gacttttctt gcccttcggc accacagcct cacgaaagcc gagttttgac
     1801 gcttccttca gacgcaaatc cggctgcgcc acctgccgaa tttccccggc caagccaact
     1861 tcgccaaaaa ataccgtctc cggcggcaac ggattattct gcaaagcgct caccagcgcc
     1921 gccgctaccg ccagatccgc agcaggctca ttaatcctga tccctccggc aatattgaga
     1981 aaaatgtcgc atgaaccaat ttgcaaaccg cagcgcgtct caagcacggc gacaatcatc
     2041 gccagccggt tcgggtccca gccaatcacg ctgcgccgcg gagacgccag cgatgacggc
     2101 gccaccaaag cctgcacctc aaccagcatc ggccgcgatc cttccagcgc cgccaaaact
     2161 gcacttcctg caacatcctg attacgctgc gataaaaaaa tctccgacgg gttttccacc
     2221 tcctgcaatc ccccgcccgc catttcaaac acgccgatct catcggtcgg gccaaaacga
     2281 tttttaacgc cgcgcaaaat acgaaattga tggctgcgat caccctcaaa atacaaaacc
     2341 gtatcaacca tatgctcaag cacccgcgga cccgctatct ggccgtcttt tgtcacatgt
     2401 ccaacaaaca tcaccgccat tccgtatgtt ttggcataac gaataatctc ctgcgccgag
     2461 ctgcgcacct gcgtcactgt accgggcgcc gaatcaatat tatcaacata cattgtctgg
     2521 attgaatcaa tgatcagcaa ttcaacaggc ccattatcac caacaggctc cacacttgcc
     2581 aaaatatcgc gtatactcgt tgcgctggcc agttgcaccg gagcttcggc cagctccaaa
     2641 cgctgcgccc gcatccgcac ctggtcaaca gactcctccc ctgacacata cacgcaacgt
     2701 gtgttctttt gcgaaagtct gcacaccaac tgtaacagca aggttgactt gccaatcccc
     2761 ggatcaccgc caatcaaaac cgctgatccc ggcaccaatc cgccgccgac aacacggtca
     2821 aattcctgaa tgtctgagac atgccgcggg taatcactgc gggccggatc actgagcgac
     2881 acgaaatcaa tcttgcgccc cttcgcgcca tccatccctt taggcactga aggcgctagc
     2941 gtttcctccg cgatgctgtt ccattcaccg cacgattcac acttaccagc ccagcgactc
     3001 gacactgctc cacaagactg acacacatat gattttttca ccttcgccat aagatcactg
     3061 tagcgaaaaa atagaacaat tcaagaacaa aatgaacgcc cccgccccaa gaggcgaggt
     3121 atcatgtcat tgcgaaccgc caaaggcggt gaagcaatcc agaatttatc cttgtggcac
     3181 tggatcgctt cgctgcgctc gcgatgacga ttacgtcgca agcggcgagg aattaaacct
     3241 gaaagagatt aaacggacaa cgtcgcgcct gcaaacaaac gcacaaaaaa taccgaccgg
     3301 tcggtatttt aaaaatttaa acgcgaaggc ttgcccataa cgctctcgag caaggctata
     3361 ttttcgacaa caagaatttt caggtcttca aattccaggt cttggtaaat aattatagat
     3421 attgcattct tacgggatgc gatgtcctaa atatagccat accgaggcgg ttaaaaacgc
     3481 taaagttcat tctaaacagc gctataaatg taaagcttgc ggcttgtcca aaaaagaaat
     3541 aaatgccggg tctggttagc ctatgaccgg gacgaatgac gtatctgcgg tgacggcggt
     3601 ttttttcagt atagagccgg cgggccaagg ccacgctgct gcgtaacagt tcttcgccgc
     3661 ctgcaagcag caacaccaaa ctcaataaaa tcggcaagac attcacgaaa aaaaacctta
     3721 ttcttattga agttaataaa gtgcacgaaa gcgatagctc atgacaattg ctgaacatgc
     3781 cctaacggaa tctattgaat ttcagatgag tctgctgctt tttatggcgc tgggcggtta
     3841 tctactttcc aatcgtattg gtcagcctgc tgttgtaggc gttattctgg cgggcatcct
     3901 gataggcccg accggtttca atcttattca ttattctgat tttgttgcca gtatcggcca
     3961 tatcggagcc attatccttc tgtttgttat tggtctggag tttcacctaa aagagattac
     4021 gaagccctcc tatttcctaa ttgctttagg cggtatcctt ctaccttggg cgggtgggta
     4081 tgcagtcgcg ttagctttcg gttttgagtt cagtaaagcc atcatcattg gcgtggctct
     4141 ttcggcaacg agtattgcaa ttaccgccga tacgctccgg gaaatgggga aactgaaatc
     4201 cgaggccgcg caggccatca taggcgcggc catcattgac gatgttctgg cgcttctggc
     4261 gctcgccatt gcccagcaat tgtctgatgg tggtgttgat gccgctgtcg tcggcgtttt
     4321 taccctcaag gctattattt ttcttgttgc cggggccctg tttggagaca aggttttaac
     4381 gccgctggtt catcggattg atcgctcaaa aattgctgaa aaatacccgg acttcatatt
     4441 catcctgatt atgatggttg cttttttcta cgctatggcc gccgagttga tggggctgtc
     4501 cgcgattgtt ggggcttttg ttgcgggcgt ttcactggag catgttcata cgcagcggag
     4561 caaggatttt cgggtgggct cggagtatct gcgcattgtt tttggtgcta ttttctttat
     4621 atcgctgggc attctggcag atttcaaaaa ctttacatgg gaggcagcgt ggtttgcgct
     4681 ggcgttaaca attgttgctg tgctgacaaa acttgtcggt tgcggtattt tggctcgatt
     4741 aagcggcatg agcgttcatg attccctagt tgtcggcgta ggtatgtcgc cgcgggggga
     4801 agtcgccatg acggttgcgc tgctggcttt tatcagcggc gttattgagc agcccgcttt
     4861 cgtgggtttg atattaatga gcctgctaac aacgattatt gcgccgcttc ttcttcgaaa
     4921 ctggttgtat cgaaacaaaa cggaagcgta aaatggcctt gcgaatgtta acgatactgg
     4981 ctccgaatgg aggtgaagaa acgcttaagg ctcttgctga agagacaaac atcattgaat
     5041 tttggtccgg cgcaaagcca aagggaaagc ccagagaaat aaatgttctg gttgcttccg
     5101 aatacttgca ggatctggtt gataagctgc aacggcgttt tagtaaggaa aaggaatggc
     5161 ggattatcat tacacctgtg gaaacaacca ttcctcgcca tgaagaaaaa gaagaggaaa
     5221 atgccaacgc caagaaagcc tatggcagcc tgaccagaga ggcgctgtat gaccaaattt
     5281 taaaaggaac aaaaaccaac acggatttta tcattctggt tgttctttca acgattgtca
     5341 cggccatcgg cctggtcacg gataatctgg ctgtgattat cggggctatg gtcatagcgc
     5401 cgctgctggg accaaattta gctctgagtt ttggggtgac gcttggcgac cgcgatatgg
     5461 tggcggaatc cctgaaggcc aatgccatgg ggtttggtct aacaatgttg ctgagtattc
     5521 tggccggtgc tctcatcccc tatgaagttt ttgcggacag ccgggaatat ctcctgcgta
     5581 caaatgtcgg ttatgacggg attattctgg cgtttgcttc cggagcggcg gctgttcttt
     5641 cgttgactgc tgggatttca agtacgatgg taggtgtcat ggtcgccgta gcgcttatgc
     5701 cgcctgctgt cgctatgggg ctatcgctgg gggaaggtgc atttgcacaa gcctatggcg
     5761 cggcccttct tctggccatt aatattatct gcgtcaatat cgcggccaag gctgttttta
     5821 cgctaaaagg catccgcccg cgcacatggt atcagcgcaa gaaatcccgc caatccctga
     5881 aaatcagttt aactttctgg gggattttac tcttgcttct tattgcgttg atatattttt
     5941 ggcaccatgg gggaacagga taaggaatgt gcttttgcgg cgggcaggtg gggtgtttag
     6001 cctataaatg cactcctgtt ttacgtgccc gtataatctc taaaacgtct tttggatggt
     6061 cggtgatgcg cacgaaatca aggtctttct cgtcgatggt gccatactcg atcatggtat
     6121 ttcttaaaaa aggccccaga gcgccccagt attcactgcc catagcaacg atcggaaaat
     6181 cacaaatctt tccggtttga atcagggttg ccgtttcaaa tatctcgtcc attgttccga
     6241 aaccgccagg caaaaggacg aaggcgcagg aatatttgag cagcatgacc ttgcggacaa
     6301 aaaagtgatc aaactgtata ttgatgtcgg tataggggtt gggtttttgt tcatgcggca
     6361 gtttgatatt gcatccgatg gatagagcgc cagcgtcttt tgccccccgg ttggcggctt
     6421 ccatgatgcc cgggccgcca cctgtcataa tcgaaaaacc tgctttgcca agctcaaaag
     6481 ccgtttccct ggccatcttg taatacggat catcttcccc aaagcgggcg gaaccgaaaa
     6541 acgtgataca atgattgatg cagcgtaagg cacgaaagcc tttctgaaat tcgcgccaga
     6601 ttttaaaaga gcgccagaga tcaaacggat aagaattttt attttctttc agtaaataac
     6661 aatcagggtc tttttgcacg ggtatcctct gttgtatagg tgggtgtcat atattattcg
     6721 tttttacagg aaaaacggtt ttaaaggaaa gaagaatgaa gctgacattt ttgggagcaa
     6781 ctggaacggt taccggttcg aaatatctgc ttgaagatga caacaaaaag atattgatcg
     6841 attgcggttt gtttcagggg atgaaggagc tgcggcaacg taactgggag aatctgcccg
     6901 ttaatcctgc ttccataaat gctgttatcc ttacccatgc ccatattgat cacagcggtt
     6961 atttaccctt gctggttaag aacggctttg acggccagat ttattgttct tccgccactg
     7021 ctgatttgtg tgatattctt ctgcctgata gtgcgcatct acacgaagaa gatgcagagc
     7081 gggccaatcg ttacggctat acgcggcaca aacccgccct gccgctttat acgcaggaag
     7141 attcctacaa cgcgcttgaa aaaatagagc ccgtttcctt tggcgatgaa catgcattaa
     7201 gcgatatact ctctttccgt ttaactcatg ccggacatat tctgggcgca tcctgtgtgc
     7261 atatttctga tgggcaaacc aatattgttt tttccggtga tcttggacgt ccgcgtgatc
     7321 cggtgatgaa ggtgccggca cagattcagg aggctgatta tctcatcctt gaatccacct
     7381 atggtgaccg cctgcatgaa acgacagatc ctctcgatca gctggaagat attatcatca
     7441 gaacatctgg gcgtggcggc acggtcgtca ttccggcttt cgccgtcggg cgcgcgcaaa
     7501 gcctgatgta ttatatctac aagctaaagg aaatgaaacg tattcccggt attccggttt
     7561 atctcgatag ccccatggct atcagcgcca cgaagctttt gcagcaacat catgctgaac
     7621 atcgcctgtc gaaggaagaa tgcgcaaagg tttgcaacag cgtgatttac acacaaaccg
     7681 tggaacaatc aaaggcgatt tatagcaaga ataacggcat gccgacggta attatttctg
     7741 ccagcggcat ggctacaggc gggcgcgtcc tgcatcacct caaacattat attagcgatc
     7801 cgagaaacac agtcctttta gccgggttcc aggcagaggg aacgcgcggc gcgcgattgg
     7861 ctcacggtga agtagaaatc aaaatccatg gcgagttgtt aagggtgcgg gctgaaattg
     7921 acatgcttga taatatctca gctcatgccg attatcagga aattatcgat tggctcgggc
     7981 atttccgcga acctccgcgc agaactttcc ttacgcatgg ggagccgaaa gcagccactt
     8041 catttaagtt caagatcaaa gagcatttag attggaacgt tgaaatcccg gaatatgaac
     8101 agatggcgga gctttagttt tggggctgtc ctaataataa cttgaaaagg ttatcagtaa
     8161 tacaacaacg tccagcgcaa aagatgccta ttatttaagg ttccaccagc cttttttctt
     8221 cgctttcggc gcctcattca cggtttcata gtccttaggg gcatcgtctt ttcttgcctt
     8281 caccgcaacc tttttgggct tggcatcatc gttggacact ttcaccacct tggcaggctt
     8341 ttcatcttta ggttcgaccg gctcatcgtt cttcttagcc ttttttacag gagctttctt
     8401 cgttgccccg tctgccatgg tcttggcgga ggcagaaggc ttcttatcgt ctttccctgt
     8461 agactcttct ttcttaaccc tgaccttttt cgaagccgcg tccactgtag ccttctcggc
     8521 ggaagcagaa ggtttcttct tctcctgagg cgctttttcg tccactttcg aatcggcttg
     8581 cactacggca ttctgtgact tctgatcggc ttgttcttca ccggcattat catctttgcg
     8641 gggctgatta cgcattccgc gattacggcc gccacggcgg ccccgtcgac gaccgtcacg
     8701 gcgattatta ccatcctgat catcctcacc agcaggtgta tcgtcgctat catcctctgt
     8761 tttatcaacg ggcttggaca cttcaacccg gaagcccgaa gccccgagcg tatcatcaac
     8821 gcggataagc acagcaaaac cgtaacgctc ttcaataaca gccagattat cgcgcttgtt
     8881 attcagaata tacagcgcca cctcattgga aacctgtaca atcacctgtg aggcacgacc
     8941 tttaatgcct tctgcttcaa tcgcgcgtag caccataata ctggccgcat cagccgtacg
     9001 tatccgtccg gcgccctgac accccggaca agtctcaaac tgactttcgc tcaacgatgc
     9061 gctcaaacgc tgacgtgaaa gctccatcaa accaaaagag gaaatccgtc ccatctgaat
     9121 tcgcgcgcgg tctccggaaa gcgcatcacg catcttgcgc tcaaccttac cgttattacg
     9181 ccggtcctcc atgtcaataa agtcaattac aaccagcccc cccagatcac gtaagcgaag
     9241 ctgccgcgcc acttcatcgg cagcttccaa attggttttt aacgccgttt cttcaatatg
     9301 acgctccttg gttgctttgc cggagttcac atcaatcgac accagcgctt cagttggatt
     9361 gatcaccaga tacccgccgg atttgagccg tacttctgtc tcgccaattt cggcaatctg
     9421 ttcctcaacc ctaaatttgt tgaacaaagg cgtatcaaca tccttatact ctttgacctt
     9481 ctttacatgc gaaggaatca tcatcttcat gaactctttt gccgtcttaa aaccttcgtc
     9541 cccggctaca tgaatttcct caatatcacg cgtatacaaa tcacgcaccg cgcgcttgat
     9601 aagatcagcc tcttcataca ccaaagccgg cgcactcgat tcaagcgtct tctcgcgaat
     9661 ggtattccaa agccgcaaaa gataatccag atcgcgcttc acttccacct tcgtacgcgt
     9721 aacgcccgct gtacgcaaaa taacgctcat acccttaggt acttccagat ccttcatcat
     9781 gtcgcgcatt tttgcgcgat ccttataatt cgaaatcttg cgcgatacac cgcccgcccg
     9841 cggactatta ggcatcaaca cacaatagcg ccccggcaaa gacaaatacg accctacagc
     9901 agcaccttta ttgccgcgct cttcttttga aacctgaacc agcataatct gaccgcgttt
     9961 gatcacttcc tggatcttgt actttcggcg caaattaaaa cggaaacgtt gctcgtcatc
    10021 catatcctcg ccgccaaaag tttctacgcg cctggcccgg tgcgctgcat tgcgtcctcg
    10081 tccgccacgt ccgcgtccat tatcacgccc gccgcgccca cggctacggc c
//
LOCUS       DAOSWR010000073        10028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4250_length_10028_cov_5.977138, whole genome shotgun sequence.
ACCESSION   DAOSWR010000073 DAOSWR010000000
VERSION     DAOSWR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_10028_cov_5.977138"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="PK513_06525"
     CDS             complement(<1..1659)
                     /locus_tag="PK513_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO82138.1"
                     /translation="MPTYTPPIDNIKFILHDVLGAAQLADLPGYEEVSDDLMNQIIEE
                     GGKICEEVLFPLNLSGDQEGCAFDDGNVTTPKGFKEAYGTFAEGGWCGLSATGEYGGM
                     GMPTLLNTVMQEMICSANMSFGMYPGLSQGAYEALHQFGTDAQKALYLPKLIEGVWSG
                     TMCLTEPHCGTDLGLIKTKAEPVDGEDSYKITGTKIFISAGEHDLTENIVHLVLAKLP
                     DAPEGARGISLFVVPKFMPNDDGSAGQHNGVACGSIEHKMGIHANSTCVMNFDEAYGQ
                     LVGEPHKGLKAMFIMMNAARLGVAMQGLGISEIAYQNGLAYAKERLQMRSLTGTKAPD
                     KPADPIIVHPDIRRLLLKSKSIVEASRALSYWTSLNLDIAEKHPDADKRQEADDLVAL
                     LTPIIKAYQTDMGLEIAVDMQQVYGGHGYIAEWGMEQYVRDARIAQIYEGTNGIQALD
                     LVGRKMPQSFGRLLRRFFHPVQKFIELNQADESLQEFIFPLAKAFAKLQQSTAIIAQK
                     GLENPDEAGAASVDYLRQFALVALAYMWCLIVQAAQKKLAEGEGD"
     gene            complement(1725..1994)
                     /locus_tag="PK513_06530"
     CDS             complement(1725..1994)
                     /locus_tag="PK513_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82139.1"
                     /translation="MFGFGKNKSDKRKKPKELQAGRVKSQRLREEALANARAARETLG
                     DETVQKIAEIIARKENNPFQQAIRQLESADSDRVADEILYMLDEK"
     gene            complement(2522..4939)
                     /locus_tag="PK513_06535"
     CDS             complement(2522..4939)
                     /locus_tag="PK513_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOO82140.1"
                     /translation="MTGINKVAVIGSGVMGSGIAAQLTNAGLPVTLLDIKVEGKDLAA
                     EAVAKMLKTEPAPFMRKANAKLITTGTIDDDLDLISDCDWIIEVVLEKLEIKHATYEK
                     IEKARKKGSIVSSNTSTIPLHKLIEGQPDSFAKDFMITHFFNPPRYMRLLELIVGEKT
                     DPKAVETIREICDVRLGKGVVQCHDTPGFIANRLGVFWLTAGVNEAIKQNVQIEVADA
                     VMSKPVGIPKTGVFGLIDLVGIDLMPKLSESLLSTLPKDDAYRDIFIDHKFVHGMIEA
                     GYTGRKGKGGFYRLNPDNPAAKEKQALAINPDSFTEDSSKGSHYIKSIKKQAENDSAV
                     SAGRKGLKAVVETDSEGGRYAWAVLRDTLSYAASLVGEIAETIFDIDEGMKLGYNWKQ
                     GPFEMIDALGPKYFAEKLAEAGRPVPEILSKVGEGTFYRIEDGKIQFFGRDGKHHDVQ
                     RPDGVLLLRDIKLSSQPVMKNASANLWDIGDGVLCFEHTSKMNTFDEQTFEMLVKAQK
                     TIESSDDYKALVIYNEASHFSAGANLGLAMFMINIAMYPQVEEFVATGQRVFMGLKFA
                     PFPVVSAPSGMALGGGCEILLSSDAVQAHAETYCGLVEVGVGLIPGWGGCKEMILRFQ
                     ERERAKFAADMSKIGKDNIWFSPDTTPVGAVRKAFETIGMATVAKSGPEAVDIGYLRK
                     TDGITMNRDRLLYDAKVRALELAKNYEPPEKVKDIRLGGAGAKMALDLAVSDLRKSGK
                     ATPYDVVVSDHLAAVLSGGDKGYTETLSEDDLLKLELSEFMHLLHNEGTFARIEHMLE
                     KGKPLRN"
     gene            complement(4959..6122)
                     /locus_tag="PK513_06540"
     CDS             complement(4959..6122)
                     /locus_tag="PK513_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82141.1"
                     /translation="MMQQNFLQVIGLKDDADISVAGTRFVECVKEYISHVEKTASKDT
                     IAKEETDVTTLYKSFFPYSVGWARQEREKNINQKNHPNAAKLKEKAASVIDDLQNNII
                     DFALCYMAIGRAIGIINAEMKRSPESNEKEVIWTSETGVMLSKQRKKRAQLLEDNKRL
                     QQAKELLEQIYPRYAAAEKAVSEIYGADQVEKLLRSYRSGLRTGNFDKARKAIEKLLT
                     EKNKFAFDKKKVEAAKDKIQAETKAYTDFLEEHREILSTGDKKLFLKPTEVDIILDAN
                     LIELAQSEAFIKKYFRPFLKFQIESLKHLRTKLLVVGSLESLMTLYIRMMRGIAEPLT
                     DIKAVRLYESEVIEHVMYLLGGQFQEVKNINARIQEILRDVSISVQNYEDVKV"
     gene            complement(6126..6386)
                     /locus_tag="PK513_06545"
     CDS             complement(6126..6386)
                     /locus_tag="PK513_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82142.1"
                     /translation="MNQIEEITKRNARVETNKAWETSWTRRVIIAAGTYLIIGGYLSF
                     LHVEGAWLHALVPVMAYMLSTLSLPLIQALWIEKIYLRNKQG"
     gene            complement(6479..7606)
                     /locus_tag="PK513_06550"
     CDS             complement(6479..7606)
                     /locus_tag="PK513_06550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOO82143.1"
                     /translation="MQTPVVICGFKRSPMHFANKGDLAKVRPDDMAAEVIKALVKDTG
                     VNAEDVEDLLMGCAFPEAEQGFNIARLVVNHADLPVSIAGATVNRFCGSSMSAIHIAA
                     GAIQMGAGQAFICAGVESMSRIPMGGFNPMPNPAIFEKYPQAYIGMGETAENLAKKYK
                     ITRAEQEEFAVASHKKAAAAAESGKFDDEIVPIGDVKTDGTIRPDSNVEKLAELKPAF
                     LEDGSVTAATSSPLTDGSAAVLVCSEEYADKHKLPKLARIKSVAVAGCAAEIMGIGPV
                     PATQKALERAGLTLDDIDIIELNEAFAAQGLSVLKELGADVSKVNLDGGAIALGHPLG
                     TSGARITGKAASLLKREKKKYALATMCIGGGQGVATVLEGV"
     gene            7796..8266
                     /locus_tag="PK513_06555"
     CDS             7796..8266
                     /locus_tag="PK513_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HOO82144.1"
                     /translation="MSVNKAAVKISDPRGFKRICMNCGARFYDMNKRPIICPSCDTEF
                     NNEMKLKGRKSRASTAQEEAEGQVTKKTSKEANGDDEDEIEEEPEDTEIVSLDDLETA
                     ETTDDDDALNVDLDDDDDDDLDDLDVDNDLDDLQDLDPDAELDEDDEGDDERKD"
     gene            8296..9204
                     /locus_tag="PK513_06560"
     CDS             8296..9204
                     /locus_tag="PK513_06560"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOO82145.1"
                     /translation="MYKVFLQLPANLSTVDVAILNESMGDLCTAHSALRTENNESAPW
                     ILEWLFDTQAEERVLSGRIETTAKIHGIDLPKILNFQNEDVPDRDWLAYSYRQFPPFS
                     VGPFFIYGAHYNDGVPDGQMGLQIDAATAFGSGEHGTTKGCLQAMLDLKGQGACPWNV
                     LDMGTGSGILAIAAWKLWKTPVLAVDNDPESVRVTQRHMEANGVPEGKGNVRTLVNEG
                     FSGSAVQDKGPYDLIIANILPSPLKEMAADLVVCTDENGAVILSGILNEQAEDVLRVY
                     LALGLEHCKTLENREWSTLLLRKTAP"
     gene            complement(9181..9498)
                     /locus_tag="PK513_06565"
     CDS             complement(9181..9498)
                     /locus_tag="PK513_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82146.1"
                     /translation="MHNTSSWLDQKPRLILLIGSVTGGLIIPFLQRFKPSVLPKKFEI
                     IYPPAILGVTAALAVSVNLIDKIFELFTKFPILSRGSEVEELYLFYFVLLYLIVLRRR
                     LAQ"
     gene            complement(9529..9840)
                     /locus_tag="PK513_06570"
     CDS             complement(9529..9840)
                     /locus_tag="PK513_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82147.1"
                     /translation="MLHKIWWLWVPLLILAAQALIELFASQKLLGEPHSESGPHEFFE
                     FIFVGAAFFVAVSTLTKLKYPQQKWLCAWVSLAAICCFYVAGEEVSWGQHFLKWSTPE
                     Y"
     gene            complement(9830..>10028)
                     /locus_tag="PK513_06575"
     CDS             complement(9830..>10028)
                     /locus_tag="PK513_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82148.1"
                     /translation="VLTMKDMPEHIPVWKKPKTMSLQSFLHKVKKIAQIARQFERTNG
                     SVYSPLVKPGLIDEEEVQDAA"
BASE COUNT         2448 a         2358 c         2525 g         2697 t
ORIGIN      
        1 atccccttca ccctctgcca atttcttttg tgcagcctgc actatcaaac accacatata
       61 ggccagcgcg acaagagcga actggcgcag gtaatctacg gaggctgcgc ccgcttcatc
      121 cggattttct aggccttttt gggcaatgat ggcggtggat tgctggagct tggcaaaggc
      181 cttggcgagc gggaaaataa attcctgcaa gctctcatct gcctgattta actcaataaa
      241 tttttgcacc ggatgaaaaa acctgcgcag caaccgaccg aagctttgcg gcattttgcg
      301 ccccaccaga tcaagggcct gaatgccgtt ggtgccttcg taaatctggg caatgcgggc
      361 atcacggaca tattgctcca tcccccattc ggcgatatag ccgtggccgc catagacctg
      421 ttgcatatcc acagcgatct ccagacccat atcggtttga tacgctttta tgattggcgt
      481 gagcaatgcc acaagatcat cggcttcctg gcgtttgtct gcatcgggat gcttttcagc
      541 aatatcgaga ttaaggcttg tccaataaga tagcgcgcgc gaagcttcaa caatggactt
      601 tgatttaagc aacaggcggc gaatatcagg gtgaacgatg atcggatcgg caggtttgtc
      661 cggcgcttta gtaccggtga gggagcgcat ttgtaagcgc tcttttgcat aggccaaccc
      721 gttttggtag gcgatttcag aaataccaag cccttgcatt gcaacgccaa ggcgtgcagc
      781 attcatcata ataaacatcg ctttgaggcc tttgtgcggc tcaccgacaa gctgaccata
      841 ggcttcatcg aaattcatga cgcaagtgga gtttgcatga atgcccattt tatgttcgat
      901 cgaaccacac gctacgccat tatgctgccc tgcgctgcca tcatcgttag gcatgaattt
      961 aggcacaaca aagagtgata taccgcgcgc gccttcggga gcatcgggca gtttagcgag
     1021 cacaagatga acaatatttt cagtgaggtc gtgttcaccg gcggagataa aaattttggt
     1081 gccggtgatt ttgtagctat cttcgccatc gactggctca gccttggttt tgataaggcc
     1141 gagatcggtt ccgcaatgcg gctcggtcag acacatcgtt cccgaccata caccttcaat
     1201 cagcttgggc aaatagagcg ctttttgcgc atccgtgcca aattggtgca gcgcttcata
     1261 agcgccttgg gagaggccgg gatacatgcc aaaactcata ttcgccgagc agatcatttc
     1321 ctgcatcacc gtgttaagca gggttggcat gcccatgcca ccatattcgc cggtcgcaga
     1381 cagaccgcac caacctcctt cggcaaaggt gccgtaggct tctttaaaac ctttgggcgt
     1441 ggtcacattg ccatcatcaa aggcgcagcc ttcctgatcg ccgctgaggt taagcgggaa
     1501 gagcacctct tcgcagatct tgccgccttc ttcgataatc tgattcatca gatcatcgga
     1561 gacttcttca tatccgggaa ggtctgccaa ttgagcagcc cctaacacat cgtgaagaat
     1621 gaatttaata ttgtcgattg gcggggtata ggtgggcata aaaaagctcc tctttttaga
     1681 ctattctacc gcgaagacgt gaaggcgcga agaggaaata aagattattt ttcatccagc
     1741 atataaagaa tttcgtcggc cacacggtca gaatccgcac tttcgagctg gcgaatagct
     1801 tgttggaatg gattgttttc tttgcgtgca atgatttcgg cgatcttttg gaccgtttca
     1861 tcgcctaaag tttcacgcgc tgcgcgtgcg ttagccagcg cttcctcccg aagacgctgg
     1921 ctcttaacac gacccgcctg aagttcttta ggcttcttcc gtttgtccga tttatttttt
     1981 ccaaatccaa acatgcttcc atcctgacag aaagggttta aaaagctgtg aacaatagat
     2041 tggattttat ctaaaaagcg gcggcgattt agggacagac ggcgtccgat tctggcaggg
     2101 cagatcaaca aaaataggct ggttgaaaga gtgaccaaga ggtggtggct gaagtttcga
     2161 acatgtagct ttttccaaag atttatttaa agatgcagcg ttggaaaaat atgttgaaat
     2221 ggcatttgga agtggttgcg ttcttgctga cgttgaggga tgggggtatg acgtttgggt
     2281 tatccagccc tgattcaact tattccctcc gcccgtgtgc atggcataaa tagcggatag
     2341 aaccccgaca atcgatgcgc tgcaagcaag gccgacatgc cacttatgtc tgcgatcaga
     2401 cacagtgttt tggtcttgct tcggttttcc agtctgcaca tttgttctca taaaaaatct
     2461 attcgcattt tcattatgag ggtcagggcc tataaattat gacgaaggct gttttcaaag
     2521 tttaattccg cagaggctta cctttttcaa gcatatgttc gatgcgggca aaggtgcctt
     2581 cgttgtgcag aagatgcatg aattcggaga gttcaagctt gagcagatca tcctcggaca
     2641 gggtttcagt atagccctta tccccaccgg agagtacagc ggcgaggtga tcggaaacaa
     2701 caacgtcata gggcgtggct ttaccggatt tgcgcaggtc tgacacggcc agatcaagcg
     2761 ccatttttgc accagcgccg ccgaggcgga tgtctttaac tttttcgggc ggctcgtaat
     2821 ttttggccag ttccagagcg cggaccttcg catcgtaaag cagacgatcg cggttcatgg
     2881 tgatgccgtc agttttgcgc agatagccaa tatctacagc ttcgggaccg gatttggcga
     2941 cagtggccat gccgatggtt tcaaaggcct tacggactgc gcctacaggc gtggtatcag
     3001 gtgaaaacca gatattatcc ttgccgatct tgctcatatc ggcagcaaat ttagcccgct
     3061 cgcgctcctg aaagcgcagg atcatttcct tgcaccctcc ccaaccgggt atgaggccaa
     3121 cgccgacctc aaccagaccg caataggttt cggcatgggc ctgcaccgcg tcagaagaaa
     3181 gcaagatttc acagccgccg cccagtgcca tgcctgatgg agcgctgaca accgggaagg
     3241 gcgcgaattt caggcccata aagacgcgct ggccggtggc gacaaattcc tcaacctgcg
     3301 ggtacatcgc gatgttaatc atgaacatag caagaccaag attggctccg gcggaaaaat
     3361 gcgaggcttc attgtaaatc acaagggctt tataatcatc gctggattcg atggtttttt
     3421 gtgccttgac cagcatttcg aatgtctgct catcaaaggt gttcattttg gaagtgtgct
     3481 caaaacacag gacgccatcg ccgatgtccc agagattggc tgaagcgttt ttcatgacgg
     3541 gctgactgga gagttttata tcgcggagca agagaacacc atcagggcgc tgcacatcat
     3601 ggtgtttgcc gtcgcgtccg aagaactgga ttttaccgtc ttcgatgcga taaaaagtgc
     3661 cttctccaac tttggagaga atttctggaa caggcctgcc tgcttcggcg agcttttcgg
     3721 cgaagtattt gggaccaagc gcgtcaatca tctcaaacgg gccttgcttc cagttgtagc
     3781 cgagcttcat accctcatcg atgtcgaaga ttgtctcggc gatttcgccg acgaggctgg
     3841 ccgcatagga cagcgtatcg cgcagcacag cccaggcata gcgcccgcct tcactgtcgg
     3901 tttcaaccac ggcttttaaa cctttacgcc cggcggagac ggcggaatcg ttttctgcct
     3961 gcttcttaat tgattttata taatgtgaac ccttggaaga atcctctgtg aagctgtctg
     4021 gatttatggc gagggcctgc ttttcttttg ctgccgggtt gtcgggattg aggcggtaaa
     4081 atccgccctt gcctttgcgg cctgtatagc cggcttcgat catgccgtgg acgaatttat
     4141 gatcgataaa aatatcgcgg taggcatcat ctttgggcag cgttgaaagc aggctttcag
     4201 agagtttagg catgaggtcg atgccgacca gatcgatcag gccgaacaca ccggttttgg
     4261 ggatgcctac gggcttgctc atcaccgcat cggcaacctc aatctggaca ttttgtttga
     4321 tcgcttcatt caccccggcg gtaagccaga agacgcctag gcggttagcg ataaagcccg
     4381 gcgtatcatg acattgcaca acgcctttgc cgaggcgaac atcgcagatt tcgcgaatgg
     4441 tttccacagc tttggggtct gttttttcac cgacaatcaa ttccagcaga cgcatatagc
     4501 gcggcggatt aaagaagtga gtgatcataa agtctttagc aaatgagtcg ggctggcctt
     4561 cgatgagttt gtgcaacgga atagtcgagg tattggacga gacgatggag ccttttttgc
     4621 gcgccttttc gattttttca taggttgcat gtttgatttc gagtttttcg agtacgactt
     4681 cgataatcca gtcgcagtcg ctaataagat caagatcgtc gtcaatcgtg ccagtcgtaa
     4741 tcagcttcgc attggctttg cgcatgaagg gcgcaggttc agttttgagc atttttgcta
     4801 cagcctcggc ggccagatct ttgccttcga ccttaatgtc gagcaaggta accggcagtc
     4861 ccgcattggt gagctgcgcg gcaatcccgc tgcccattac gcctgatccg attacggcga
     4921 ctttattgat ccctgtcatg gtgaggtcct ttcattgctc atacttttac gtcttcataa
     4981 ttttgtacgc ttatactcac atctcgaagt atttcctgaa tgcgcgcatt gatatttttg
     5041 acttcctgaa actgtccgcc caaaagatac atcacatgtt caataacttc cgattcataa
     5101 agccggacgg ctttaatatc tgtgagtggt tcagcaatac cacgcatcat acgtatataa
     5161 agtgtcataa ggctttcgag cgagccgaca acaagcaatt tagtccgtaa atgtttcaag
     5221 gactcgatct gaaatttcag gaacgggcgg aaatattttt ttatgaaggc ttcgctttgc
     5281 gcgagctcta ttaggtttgc atcaagaatt atgtccactt ctgttggctt taaaaataat
     5341 tttttatccc cggtgcttaa gatttcccgg tgctcttcaa ggaaatctgt ataggctttt
     5401 gtttcagcct gaattttgtc ttttgcagct tcaaccttct tcttatcgaa tgcaaatttg
     5461 tttttttctg ttagcaattt ttctatggct ttgcgtgctt tgtcaaaatt tcctgtcctc
     5521 aaaccagagc gataagaacg caggagtttt tctacttggt ctgctccata tatttcagat
     5581 acggcttttt ccgctgctgc atagcgcgga tatatttgtt ctaaaagttc ttttgcttgt
     5641 tgtaagcgct tgttgtcttc gagaagttgt gctctttttt tccgctgctt gctgagcatt
     5701 acgcctgttt cactggtcca gatcacttct ttttcattgc tttcagggct gcgtttcatc
     5761 tcagcgttaa taatgccgat agccctgcca atcgccatat agcacagcgc aaaatcaata
     5821 atgttatttt gcagatcgtc gataacgctt gcagcttttt cttttagttt tgctgcattc
     5881 ggatggtttt tctggtttat gtttttttct ctttcctggc gcgcccatcc taccgagtat
     5941 ggaaagaagc ttttgtaaag tgtcgtaacg tctgtttctt ctttggcaat tgtgtctttg
     6001 gacgccgttt tttcaacatg gctgatatat tctttcacgc attcaacaaa gcgtgtacca
     6061 gcaacgctta tatcggcgtc gtctttaaga cctatgacct gaagaaaatt ctgctgcatc
     6121 atgttttagc cttgcttatt tcgcagatag attttttcaa tccatagagc ctgtatcaat
     6181 ggcaatgaaa gtgtcgaaag catataagcc ataactggaa caagcgcgtg aagccaggcg
     6241 ccctcgacgt gcaaaaaact taaatagccg ccgatgatca gatatgttcc cgcagcgata
     6301 atgacgcggc gtgtccatga ggtttcccat gccttgttgg tttcaacgcg ggcattgcgc
     6361 tttgttatct cctctatctg attcattcgt cattgcgagc gcagcgaagc aatccagctc
     6421 tgtagatgga ttgccgcgtc gggcttcgcc ctcctcgcaa taacgataaa aaattcactt
     6481 aaaccccctc cagaacagtc gcgacgcctt ggccaccgcc gatgcacatg gtagccagcg
     6541 cgtatttctt tttctcgcgc ttcagcaggc ttgcggcttt gccggtgatg cgcgcgcctg
     6601 aggtgcccag cgggtggccc agcgcgatag cgccgccgtc gaggttgact ttgctgacat
     6661 cggcgccaag ttctttcagc acagataacc cctgcgcggc gaaggcttcg ttgagttcga
     6721 taatgtcgat gtcatccagc gttagccctg cgcgctccaa tgctttttgc gtggcgggta
     6781 cggggccaat tcccatgatt tccgcggcgc agccagcaac ggcgacagat ttgatccgcg
     6841 cgagtttggg gagtttgtgt ttatcggcgt attcttcgct acacaccagc actgcagcag
     6901 agccatcggt gagcggtgag gacgttgcag cggtaacgct gccgtcttcg aggaaggcgg
     6961 gcttaagctc ggccagtttt tcaacattcg aatcagggcg gatggtgccg tcggttttga
     7021 catcgccgat aggcacgatt tcatcatcaa acttgccgga ttcggccgca gcggcggctt
     7081 ttttgtgaga agcaacagcg aattcttcct gctcggcgcg ggtgatttta tatttttttg
     7141 ccagattttc agccgtttcg cccatgccta tataggcttg ggggtatttc tcaaaaatcg
     7201 ctgggttggg catcgggtta aagccgccca tgggaatgcg gctcatggat tctacgcctg
     7261 cgcagataaa ggcctggccc gcacccattt gaatagcacc ggcggcgata tgaatggcgc
     7321 tcatggacga accacaaaag cgattgacag ttgcgcccgc gattgagact ggtaaatccg
     7381 catgatttac aaccaggcgc gcgatgttaa agccctgctc ggcttcagga aaagcgcaac
     7441 ccatcaataa atcttcgacg tcttcggcgt tcacgcctgt atctttgact agggctttga
     7501 tgacttcggc agccatatca tcaggacgca cctttgccag atcgccctta ttagcgaaat
     7561 gcatgggaga gcgtttaaag ccacaaatga cgacaggtgt ttgcatggga agaacctccg
     7621 gtaagaataa attttcatga ttctataatg atataaaggc cgggtcgaga tgttttaaga
     7681 gcatggagag attaagccct gggacgagtt tgattttgca tttgacacac ctgataaaat
     7741 caggcaattc agatttaaag attgaatgat ttttaatgcg tttggcacaa ggagaatgag
     7801 cgtgaataag gctgctgtga agatttctga tccacgggga tttaagcgta tttgcatgaa
     7861 ttgcggggcg cgtttctatg atatgaacaa acgtccgatc atatgtcctt catgcgatac
     7921 ggagtttaac aacgagatga agcttaaggg ccgtaagtcc cgcgcgagca cagctcagga
     7981 agaggctgaa gggcaagtta cgaagaagac ctctaaagaa gccaacggcg atgacgagga
     8041 tgaaattgaa gaggagccgg aagacactga aattgtcagt cttgatgatt tagaaactgc
     8101 agaaacaaca gatgatgatg acgcgctaaa tgtcgatctt gacgacgatg atgatgacga
     8161 tcttgatgat cttgatgtcg ataatgattt ggatgattta caagatctcg accctgatgc
     8221 tgagcttgac gaagatgacg agggcgatga cgaaagaaag gattaacgct ttgcttatca
     8281 gtaaaggata gttgtgtgta taaagttttc ctgcagcttc ctgccaattt gagcaccgtt
     8341 gatgtagcca ttttgaacga atctatgggt gatttatgca ccgcgcattc ggctctgcgc
     8401 acagaaaaca acgaaagcgc gccgtggatt ttggaatggc tgtttgatac gcaggctgaa
     8461 gagagggttt tgagcggtcg gattgaaact acggccaaga ttcatggtat tgatttacca
     8521 aaaattctta attttcaaaa cgaagacgta ccagaccggg attggctggc gtattcctat
     8581 cgacaatttc cgcctttttc agtcgggccg ttttttattt acggcgcgca ttataacgac
     8641 ggcgttcctg atggacaaat gggtttgcag atcgatgccg ccacggcatt tggatcgggt
     8701 gagcatggca cgaccaaagg ctgcttgcag gcgatgctgg atttgaaagg acaaggggca
     8761 tgcccgtgga atgtgctgga tatgggtaca ggcagcggca ttctcgcgat tgcggcatgg
     8821 aaactttgga aaacgccggt actggcggtt gataacgatc ctgaaagcgt tcgcgttacg
     8881 cagcggcata tggaagctaa cggcgtgcct gagggcaagg gcaatgtgcg aacgctggtc
     8941 aatgaagggt tttcagggag cgctgtgcag gataaaggcc cgtatgattt gattattgcg
     9001 aatattttgc ccagcccgtt gaaagaaatg gccgcagacc ttgtggtctg cacggatgaa
     9061 aacggcgcag tgattttgtc gggcattctt aacgaacaag cagaagatgt actcagagtt
     9121 tatctcgcat tggggctaga gcattgcaag acgctggaaa atagggaatg gtcaacatta
     9181 ttactgcgca agacggcgcc gtaagacgat cagataaagc agcacgaaat aaaagagata
     9241 gagttcctca acctcactgc cgcgtgaaag aataggaaat tttgtaaaaa gctcaaagat
     9301 tttatcaatt agatttacgc ttacagccag agctgccgta acccccagga ttgcaggtgg
     9361 ataaataatc tcaaattttt tgggtaatac tgagggttta aaacgctgta aaaaaggaat
     9421 gatgaggcct ccagtaaccg aaccaatcag taaaattaag cgtggttttt gatcgagcca
     9481 gcttgatgta ttgtgcaaat tggtttcgcc ctgatcgttg atatgagctc aatattcagg
     9541 cgttgaccat tttagaaaat gctgccccca actcacttct tcacccgcta catagaagca
     9601 gcagatcgcc gccagactga cccaagcaca aagccatttt tgttgaggat acttgagctt
     9661 tgtaagtgta ctcaccgcaa caaaaaatgc ggcaccaaca aaaataaatt cgaagaattc
     9721 atgcgggccg gattctgaat gcggctctcc caaaagcttc tgcgaagcaa aaagttcaat
     9781 aagggcttgt gcagctaaaa taagcaacgg gacccaaagc caccaaattt tatgcagcat
     9841 cttggacctc ttcttcatca ataaggccgg gcttcacaag aggggagtac acagacccgt
     9901 tcgttctttc aaattgccga gcaatttgcg caattttttt taccttgtgt aaaaaactct
     9961 gcaacgacat ggtcttcggc tttttccaaa ctggaatatg ctccggcata tccttcattg
    10021 tcaaaact
//
LOCUS       DAOSWR010000074         9985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4277_length_9985_cov_5.923968, whole genome shotgun sequence.
ACCESSION   DAOSWR010000074 DAOSWR010000000
VERSION     DAOSWR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_9985_cov_5.923968"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PK513_06580"
     CDS             complement(<1..495)
                     /locus_tag="PK513_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO82149.1"
                     /translation="MLDRKDILAALKSVEDPERGIDIVSAGMVPDVQLGEDGQVLFMM
                     EVDPQRGSALEPLRQQAEQVVSALEGVRKVTAVLTAERAAEKVPGEKFAANDFAMDPH
                     RMAKNPPLKLPIKKIIAVASGKGGVGKSTVAAGLARALVAQGLKVGLLDADIYGPSQP
                     KLMGV"
     gene            602..1750
                     /gene="hflK"
                     /locus_tag="PK513_06585"
     CDS             602..1750
                     /gene="hflK"
                     /locus_tag="PK513_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOO82150.1"
                     /translation="MGKDDKDKKNPWGSKRPGNDDRGPWGVGKSGGRSGSGSGGETPD
                     IDEILRKAQENFRAVMPGGFSGAPIIGIAIIGVLVLWIASGFYVVNPGENAVIQRFGA
                     WSRTQAEPGLGYHLPSPIETASILNVEEIRKMNIGFMEAISRNGASNLRDLPEESLML
                     TSDRNIVDLDMVIQWNIKSAEDFLFEIRDQELIIKSIAESAIREVVGQTDMDPIITTG
                     KENVVTATKKIIQENLDRYKSGVNVTDVLFKSAPQVHPDVQAAFQDVQAAKQDAKDVQ
                     NRAGAYREDILPKARGQAIKMLQEAQAYKKSTVARSTGDADRFNAVYQAYLTGKDVTK
                     ERIYIETMENVLGNAQKIILDSKAGQGAVPYLPLNQLNTVKPAAGSEN"
     gene            1763..2710
                     /gene="hflC"
                     /locus_tag="PK513_06590"
     CDS             1763..2710
                     /gene="hflC"
                     /locus_tag="PK513_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOO82151.1"
                     /translation="MNKPLLIILALIAIGLIGASQVFFIVDQREQAIVLQLGQPVGDV
                     NGPGLHVKIPVIQTVQYFDRRILSVDPDPEQVNVSSVDTPKSSKNKETQKENDSDNLS
                     PVDVTGEPIILDTFARYKITDPLQFMKTLRTISAANSRIENILNDATNAVVGKISLRR
                     LLSPDRTMIMQDIMKRVSTSVKKSNFGIEIVDVRIVRADLTPDLRTSTVRRMISELKK
                     QATETRAKGEERALEIRSTAEKERTVLIAEAQRDASILKGEGDEEAIKIYANAFNKDK
                     EFYSFIRSMEAYKNTLADPATQLILSPDSEFFKHFDDKR"
     gene            2742..4214
                     /locus_tag="PK513_06595"
     CDS             2742..4214
                     /locus_tag="PK513_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOO82152.1"
                     /translation="MTLQKAPKFILPLILCACLMLSVSIKANARMLGQNGPDSFADIV
                     DDLLPAVVNISSTQKIDVSEDVPDLPQFPEGSPFQDFFEEFMERRHGMPSIPQASLGS
                     GFVIDAQNGYIITNNHVVKDAEEIRVTFQDDETLPAELIGTDEKTDLAVLKIDTKKKL
                     TAVKFGNSDVLRVGDWIVAIGNPFGLGGTVTAGIISARQRDINAGPYDDFLQTDASIN
                     RGNSGGPMFDLKGEVIGINTAIFSPSGGSVGIGFAIPSALAKPVINQIIQYGRTRRGW
                     LGVRIQQVTDEIAESLGVEKATGALIASITPGGPAEKAGLEAGDIILEFNNQKVHEMR
                     ELPRLVAEAKIDAPATITYWRDNKKTTTEVKVGELEKAEEDGLIASGEEETSKGTKVD
                     SVGLSLKKITDTMRRDYAIPPSVDGVAIVSVDPKSEAAQKGLFEGDIIVEINQTPVSD
                     PEKAKEIIEKADENGRSSILLLINREDEVRFLALKLKKDK"
     gene            4227..5102
                     /gene="miaA"
                     /locus_tag="PK513_06600"
     CDS             4227..5102
                     /gene="miaA"
                     /locus_tag="PK513_06600"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOO82153.1"
                     /translation="MGKKSKAILHIVAGPTASGKSAKAMELACAMNGVIINCDSQQIY
                     DGLPILSAQPSDEDKAAVPHKLYAFLHPNDVCSAGNYRELAQPIIEETLAAGQTPIVC
                     GGTGLYIKALTEGLSPMPDIPEDVRERVVTKYENIGAEKFYAELEARDPEMAARFHVN
                     HKARIIRAMEVLEATGKSLAIWQKEDRLSPPDHWHFEIHKILPERTALYKRCNERFIW
                     MAENGALEEVEEFDARLQSGEVKDGVPLCKALGFKELRAYLNGEINKDEAIEKAQGIT
                     RRYAKRQITWFRNQL"
     gene            5255..5809
                     /locus_tag="PK513_06605"
     CDS             5255..5809
                     /locus_tag="PK513_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleosidase"
                     /protein_id="HOO82154.1"
                     /translation="MKPLLVFAMKEESQDVFVDYDVLHCQIGKINASHNLMRQIAQNR
                     PELVVNLGTAGSRKHAGGTIVNPTQFIQRDMDVTALGIELYQTPFSDDPIIIEHGLRF
                     EHLPGGICGTGDNFDASQAAATFDVVDMEAYALALICKRENIPFACLKYVSDGANDDA
                     HMDWHEALHITAEKLHKALKGANL"
     gene            5806..6516
                     /locus_tag="PK513_06610"
     CDS             5806..6516
                     /locus_tag="PK513_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82155.1"
                     /translation="MSIKSWNILLVLALLIVLSGGGYILYQNNQQKQAEIAKAQAEEQ
                     ARIAEETQRKKQEQTALMQSFEDFLNNFLQDINTQVHEYKQARTVMDELLKPGNQSTP
                     EYIIENARLAEGTVMSLQLQMEDILKQFEKANANLKPLIEKLTPDSQDHVRQTWNKIR
                     NENAEKFMTFFELDQEVLSAQLKLIEFYKSHAQILHTDIENKRITFDDITLQEQEAML
                     RGKIMAAKATQKNLFKKI"
     gene            complement(6558..8003)
                     /locus_tag="PK513_06615"
     CDS             complement(6558..8003)
                     /locus_tag="PK513_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOO82156.1"
                     /translation="MKLQIVWYMLGVLVAVVGLAEMVPAIVDWCLGHENARDFFLNGI
                     LCLFFGGSLILSYRVRDRQVNAHQMFLMALSGWLVVSVFCAMPLYMSDLGLSFVEALF
                     EAVSGVTTTGATVLINLEETSRGVLLWRSMMQWAGGLGVIAFAVVLLPYLKVGGMQLF
                     RSESSGHSDKVVSRSAQFLGGLLKVYIAMTLVCAGVYYLLGMSGFDAFNHAMTTLSTG
                     GFSTHDASFGYFKSSALQYAAVFFMVLAAFPFVLYLRLIYQRRVLFFRDEQVRALMLC
                     LCLAVFSLTLWLWAVTPYTLEVSFRMIVFSVSSIMSTTGYTIADYTLWGGFTTLAFLL
                     MMYVGGCAGSTAGGMKVMRIVISAKVVVRQFRTLLYPNGVFVLNYQGRRLDSYTVLTV
                     LGFLSLYVGANVLMTVALALTGLDFETSMSAAAAALANVGPGIGGQVGPTGNYAGLSD
                     TAKWILCAGMIAGRLEILTILVLFSPSYWRA"
     gene            8656..9981
                     /locus_tag="PK513_06620"
     CDS             8656..9981
                     /locus_tag="PK513_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82157.1"
                     /translation="MAEQGMRSISDKVFNHVARDNPIVVEEDHEIQSILSYCVQQKDL
                     HIVNYLVIDENGQEQEQEDFPIEDYKTKKIIGLSFGKKGEELKRFRFKFCRNHNFEAL
                     NYHGMPEYDTVQINYASPASYDFFVKGYTNDTTGETKPGYWKQVIDNDIDLGFTGLRF
                     DIAYKIPTPWLSELIQYAHDRQPEIMTIAETLAGVDDIGAKELIPKLAEAKILVDGVE
                     RPAIDHAMLSTYWAGPLDGWLIDESRLMAGISRYGGAGSPDNHDTQGTVAQNIAKMLE
                     HYAPEDKERAIADICVRDYALAALVGNAHYAQMGYEVCADQAGVFEEDTNPKQWRMLL
                     AEREASDHPLNISERLWEINKFKASLNADNAIFQLESTAPLSEGSNLVKMVISLTDQE
                     TKQHMGTLDLFINNKPECGPVELPNENTAFILTNRGGPKNSDSELSYDL"
BASE COUNT         3139 a         2709 c         2036 g         2101 t
ORIGIN      
        1 cacacccatc agcttcggct gcgaaggacc ataaatatcc gcatccagca aaccaacctt
       61 aagcccttgt gctaccagcg cccgcgccag cccggccgcc acggttgact ttccgacccc
      121 gcccttacca gaggccaccg caataatctt cttaatcggc aattttaatg gcggattttt
      181 tgccatcctg tgcggatcca tagcaaaatc attcgctgca aatttttctc caggcacttt
      241 ttccgccgcc ctctctgctg taagcacggc tgtgactttc cgcacacctt caagcgccga
      301 aacaacctgc tccgcctgct gacgtaacgg ctcaagcgca cttccacgct gtggatcgac
      361 ctccatcata aacaatacct gcccgtcctc accaagctga acgtcaggca ccatccccgc
      421 actgacaata tcaataccac gctccggatc ctcaacgctc ttgagcgccg ccagaatatc
      481 ttttcgatca agcatatttt ttaaaccttc aaacccttgc tttcagcctc aaatagaata
      541 ttgtaagtag ccatatactt ttgaatggat tgagatcaac taaaatcggg aaaaagacct
      601 tatgggcaag gacgacaaag acaaaaaaaa cccttggggc tccaaacgcc ctggtaatga
      661 cgaccgtggg ccttggggcg taggtaaatc aggaggtcgc agcggatctg gcagtggcgg
      721 cgagactcca gatattgatg aaatcctgcg caaagcccaa gaaaatttcc gcgccgttat
      781 gcccggcggt ttttctggcg cgcccattat aggcattgcc atcatcggtg tactcgtcct
      841 gtggatagcc agcggattct atgttgttaa ccccggtgaa aacgcagtca ttcaacgctt
      901 tggcgcatgg tcccgcacac aggccgaacc tggtctgggc tatcacctgc cctccccgat
      961 cgaaaccgcc agtattttaa atgtcgaaga aattcgtaaa atgaacatcg gcttcatgga
     1021 agctatttcc cgcaacggcg cttccaacct ccgcgatcta ccggaggaaa gcctgatgct
     1081 gacctcggac cgcaatattg tggatctgga tatggtcatt caatggaaca taaagtccgc
     1141 cgaagatttc ctctttgaaa tccgcgatca ggaactaatc attaaatcaa tcgccgaaag
     1201 cgccattcgc gaagttgtcg gccagacaga tatggaccct atcatcacaa ccggtaaaga
     1261 aaatgtagta acggcaacca agaaaatcat tcaagaaaat ttggaccggt ataaatccgg
     1321 ggtcaatgta acggatgttt tgttcaaaag cgcgccacaa gttcatccag acgttcaagc
     1381 agcctttcag gacgttcaag cagccaaaca agatgcaaaa gatgtgcaaa accgtgctgg
     1441 agcctaccgc gaagacatcc tgcccaaagc tcgcggtcaa gccatcaaga tgctgcaaga
     1501 agcgcaagct tataaaaaat ccacggttgc acgctcaaca ggggacgcag atcgctttaa
     1561 cgcagtctat caagcctacc tgacaggtaa agatgtcacc aaagaacgca tttacatcga
     1621 aaccatggaa aatgttctgg gcaacgcaca gaaaattatt ctcgatagca aagctggaca
     1681 gggggctgta ccctacctgc cgcttaatca gttaaacacc gtcaaacccg ccgccggatc
     1741 tgaaaattaa aaggataaaa tcatgaacaa accactcttg atcatattag ccttgatcgc
     1801 catcggcctc atcggcgcgt cacaagtgtt ttttattgtt gaccagcgtg agcaagccat
     1861 tgtccttcag cttggtcaac cggtcggtga cgtcaacggc cccggcctgc acgtgaaaat
     1921 tccagtcatt caaacggtgc agtattttga tcgccgtatc ctgtccgttg atccagatcc
     1981 ggaacaagtc aacgtttcca gcgtggatac accgaaatct tccaaaaaca aagagacaca
     2041 aaaggaaaat gatagcgata atctatcacc tgtcgatgtg accggagaac caattattct
     2101 tgatacattt gcacgttaca aaatcacaga tcccttgcaa ttcatgaaaa ccctacgcac
     2161 aattagtgcg gcaaattctc gtattgaaaa tatcctcaat gacgcaacaa acgccgtggt
     2221 aggaaagata agtttgcggc gcctcctttc gccagataga accatgatca tgcaagacat
     2281 catgaaacgt gtaagcacca gcgtcaaaaa aagcaatttt ggcattgaga ttgtcgatgt
     2341 ccgaatcgtt cgtgcggacc tgactcccga tctacgcacc tcgaccgtcc ggagaatgat
     2401 ctccgaactc aaaaaacagg ccacggaaac ccgtgcaaaa ggtgaagaac gcgcccttga
     2461 aatccgttca acggcggaaa aagaacgtac cgttctcatt gccgaagccc agcgtgatgc
     2521 ctctatcctc aaaggggagg gcgatgaaga agctatcaag atttacgcca acgcctttaa
     2581 taaggacaaa gaattttatt cgttcatccg ctcgatggaa gcctataaaa atacgttagc
     2641 cgacccggcg acgcagctta tcctctcacc agacagtgag ttctttaagc attttgacga
     2701 caaacgttaa acaacatctc cgaaggaatg aggatcatac tatgacgttg cagaaagcac
     2761 caaaatttat acttcccctc atactctgtg catgcctcat gctatccgtg tcgataaagg
     2821 ccaatgcacg catgctaggg cagaacggcc cggacagctt cgccgacatc gtcgatgatc
     2881 ttctccctgc cgtcgtcaat atctcctcaa cccaaaaaat cgatgtgtct gaggatgtac
     2941 cagacctacc tcaatttcct gaaggctctc cttttcagga cttttttgaa gaatttatgg
     3001 aacgccgcca cggcatgccc tccataccgc aagcatccct cggttccggc ttcgtcattg
     3061 atgcgcaaaa cggctatatc atcacaaata accacgtcgt caaagacgcc gaagaaatcc
     3121 gtgtaacttt tcaagacgat gaaacgctgc ccgcagaact catcggcacc gatgaaaaaa
     3181 cagatctggc agttctcaaa atcgatacca agaaaaaact gacagccgta aagtttggca
     3241 acagtgatgt cctgcgcgtc ggcgactgga ttgtagccat cggcaacccg ttcggacttg
     3301 gtggcactgt gaccgccgga attatttccg cccgccagcg cgacatcaac gccggccctt
     3361 atgatgattt cctccagacc gatgcctcca tcaaccgtgg caactcaggc ggcccgatgt
     3421 tcgacctcaa aggtgaagtc atcggcatta acacagctat tttctctcca tcaggcggca
     3481 gcgtcggcat cggctttgca attccttccg cgctcgccaa acctgttatc aatcaaatca
     3541 tccaatatgg ccgtacgcga cgcggctggc tcggtgtacg catccagcaa gtcaccgatg
     3601 aaatcgctga aagtcttggc gtagaaaaag ccacgggcgc gcttatcgcc tccattacac
     3661 ccggcggccc ggcagaaaaa gcgggcctgg aagctggtga catcatcctt gaatttaaca
     3721 accaaaaagt ccacgaaatg cgagagcttc cccgcttggt cgccgaggca aaaatcgatg
     3781 ctcccgcaac aatcacctac tggcgtgaca acaaaaaaac caccaccgaa gtaaaggtcg
     3841 gcgaactcga aaaagctgaa gaagacggtc tcattgcctc tggagaagaa gaaacatcga
     3901 aaggcaccaa agtagacagc gtcggcttat ccctgaaaaa aattactgac acgatgcgcc
     3961 gcgattacgc tatcccgcca tccgttgatg gcgtcgccat cgtcagcgtt gatccaaaat
     4021 cagaagccgc gcaaaaaggc ttgtttgaag gcgatataat tgtcgagatc aaccagacac
     4081 cggtttcaga tcccgaaaag gccaaagaga tcatcgaaaa agccgatgaa aatggccgct
     4141 catcaatatt gttgctcatc aaccgcgaag acgaagtccg cttcttggcc ttaaagctga
     4201 aaaaagataa gtagacactc aaaaccttgg gtaagaaaag caaagccatc cttcacatcg
     4261 tcgccggacc gaccgccagc ggtaaatccg ccaaggccat ggaactggcc tgcgcaatga
     4321 acggcgtcat catcaattgc gattcccagc aaatttatga cggcctcccc attctctccg
     4381 cccagcccag cgatgaagac aaggccgcgg tccctcacaa actctatgcc ttcctgcatc
     4441 ccaatgatgt ctgcagcgcc ggaaattacc gcgaactggc ccagcccatc atcgaagaaa
     4501 cattggccgc cggacaaacg cccatcgtct gcggcggcac gggactatat atcaaagcac
     4561 tgaccgaagg cctctccccc atgcctgaca tcccagaaga cgtgcgcgaa cgcgtcgtca
     4621 ccaaatacga aaacatcggt gccgaaaaat tctacgccga acttgaagcc cgcgatcctg
     4681 aaatggctgc acgctttcat gtcaatcaca aggcccggat catccgcgca atggaagtat
     4741 tggaagccac cggaaaatcc cttgccattt ggcaaaaaga agatcgcctc agccctcccg
     4801 atcattggca tttcgaaatc cataaaatcc tgcccgaacg cacggcgctc tataaacgct
     4861 gcaacgaacg ctttatctgg atggctgaaa acggcgcact cgaagaggtc gaagaattcg
     4921 acgcccgcct tcaatccggc gaagtcaaag acggtgtacc tctgtgcaaa gcgctgggtt
     4981 ttaaagaact tcgcgcttat ctaaacggcg agataaacaa agacgaagcc atcgaaaagg
     5041 cccaaggcat cacccggcgt tacgccaaac gccagataac atggtttaga aatcagcttt
     5101 agccaatgta atctcgctta attagaacct tttcaaggtt attccctcat cctcgataca
     5161 tcaaatatca ctgctcgaaa atttcagtgg caacgttatc aataagcttt tgtctcttta
     5221 atatccgctc acttcgtgct aatcatcaaa catcatgaag cccttacttg tttttgcaat
     5281 gaaagaagaa agtcaggatg tctttgtgga ctacgatgtc ctgcattgcc aaattggcaa
     5341 aataaacgcc tctcacaatc tgatgcgcca gatcgcgcaa aatcgcccgg aattggtggt
     5401 caatcttggc accgcaggca gccgcaagca tgcaggcggt acaatcgtca accccaccca
     5461 atttatacag cgtgatatgg atgtcacagc ccttggcatt gaactctacc aaacgccgtt
     5521 ttccgacgat cccataatca tcgaacatgg tctgcgtttt gaacacctgc ctggcggcat
     5581 atgcggcaca ggggataatt ttgatgccag ccaggccgct gcaacttttg atgtcgtgga
     5641 tatggaagca tatgccttag ccttaatttg caaacgtgaa aatattccct ttgcctgctt
     5701 gaaatatgtt tcagatggtg ccaatgatga cgcccatatg gattggcatg aggcactgca
     5761 cattacagcc gagaaattgc acaaagcttt aaaaggagca aatttgtgag cattaaatcc
     5821 tggaatattc ttttggtttt agccctgtta atcgtgctca gcggcggcgg atatatcctg
     5881 tatcaaaata accagcaaaa acaggctgaa attgctaaag cccaagctga agaacaagca
     5941 cgcattgccg aagaaacgca gcgcaaaaag caagaacaaa cagccctgat gcaaagcttt
     6001 gaagacttcc tcaacaattt cctgcaagac attaacacac aagtccacga atataagcaa
     6061 gcccgcacag tcatggacga attgctaaaa ccaggcaatc aaagcactcc tgaatatatc
     6121 atagaaaatg cccgcctggc ggaaggaacc gtaatgtctt tgcagctaca aatggaagac
     6181 attctcaaac agtttgaaaa agcaaacgcc aacctcaagc cgctcattga aaaactgacc
     6241 ccagactccc aggaccatgt gcgccaaacc tggaacaaaa ttcgcaatga aaatgctgaa
     6301 aaattcatga cttttttcga attagaccag gaagttctat ccgcacaact caagctgatt
     6361 gagttttaca aaagccacgc acaaattctt cataccgata tagaaaataa acgcattacg
     6421 ttcgatgata ttacccttca agaacaggaa gccatgctcc gtggcaaaat aatggcagca
     6481 aaagcaacgc aaaagaatct tttcaaaaag atctaagcac ccttttcaaa aacgtgatta
     6541 ccagcagtat ttttatgcta agcgcgccaa taagacggtg aaaacaacac aagaatagtc
     6601 aaaatctcaa gccgaccggc aatcatgccc gcacataata tccatttcgc cgtatcagac
     6661 aaacccgcat aattgcctgt tggaccgacc tgcccaccaa ttccaggccc aacattagcc
     6721 aaagctgccg ccgccgcact catcgatgtt tcaaaatcaa gccccgttaa cgccaaagca
     6781 acagtcatta aaacatttgc ccccacatac aaacttaaaa aaccgagcac cgtcaaaaca
     6841 gtataactat ccaaccggcg cccctgataa ttcaaaacaa acacgccgtt agggtaaagc
     6901 aaggtcctga actgacgcac caccaccttg gctgaaataa ctatgcgcat gaccttcata
     6961 ccaccagccg tagatcccgc acatccaccc acatacatca tcaacaagaa tgccagcgtt
     7021 gtaaaaccgc cccaaagcgt ataatcagca atcgtatatc cggttgtgct cataattgaa
     7081 gaaacactga aaacaatcat gcggaaactg acctctaacg tataaggcgt aacagcccac
     7141 aaccaaagag taagagaaaa aaccgccaaa cataggcaca acatcaacgc acgcacctgc
     7201 tcatcacgaa aaaacaaaac acggcgttgg taaataagac gcaaatacaa cacgaaagga
     7261 aacgctgcaa gtaccataaa aaatacagcc gcatattgca gtgccgaact tttaaaataa
     7321 ccaaaagagg catcgtgtgt tgaaaacccg cccgttgaaa gtgttgtcat tgcatggtta
     7381 aaagcatcaa aaccactcat ccctaacaaa taatatactc cggcgcaaac caacgtcata
     7441 gcaatgtaaa ctttaagtaa ccccccaaga aactgcgcac tacgtgacac caccttatcg
     7501 gaatgccctg aggactcact acgaaacaat tgcatgccgc caactttaag ataagggagc
     7561 aatacaacgg caaaagcaat aacacccagc cctccggccc attgcatcat cgaacgccat
     7621 aacaaaacac cacgcgaagt ctcctccaga ttaatcaaaa ccgtagcgcc ggtagtcgtc
     7681 accccggaaa cagcctcaaa cagagcctca acaaaactta aacccaaatc cgacatataa
     7741 agcggcatcg cacaaaacac ggatacgacc aaccaaccgc ttaaggccat caaaaacatc
     7801 tgatgagcat tcacctgccg atcacgaaca cggtatgaaa gaataagact tccaccaaaa
     7861 aacaaacaca atatgccatt aagaaaaaaa tccctggcgt tctcgtgccc caaacaccaa
     7921 tcaacaatag ccggaaccat ttcggccaag ccgacaacag caacgagaac acccagcata
     7981 taccaaacaa tctgcagctt catatcccat ccatacacga ttaatgtctg caaaaaaaca
     8041 accttctaaa aacgcagaac acaccgttct ataaaccatt aaaatgctat gaaattcaaa
     8101 gcaagtacaa gcaagttgat tttgggaaaa cacgccgatt acaaataaat tcatggcttt
     8161 ctcaaatata ttaaccacct ataagaacaa caaaaaatca gcgcacatat tgccttgcac
     8221 aataaaaagt cgtgggaaaa ccaacaaaat actagaatat tccaataaac aaatataaaa
     8281 agtaagataa ttacaaaata tggagtgcag ggaacaaaaa ttgcgtaaca cgaatccgat
     8341 tatctacaac gtaaacccca gaaaaatagg ccctattaga gaatggtccg gagctaaccg
     8401 cgctgatggc ctatccccac tcattaaaag ggccaaagaa cttggttaca acgcgatctg
     8461 gttcaaccct ctctaaccgc acccacaaga atctcattca gggatgactt ccagcaaaca
     8521 catagtcgaa gtctttatgc aatcagaaat cattttcgtt ttagtgccga gctaagtgcc
     8581 caccctaatc cgacagcccc atcatacatt cgctcgtttc tggaccgcat ccacattaag
     8641 cattttaatc agaaaatggc agaacaaggc atgcgctcta tttcagacaa ggtttttaac
     8701 catgtcgccc gcgacaaccc catcgtcgta gaagaagacc atgaaataca aagtatttta
     8761 tcttactgcg tgcaacagaa agacctgcac atagtgaact accttgtcat tgatgaaaac
     8821 ggccaagaac aggagcagga agactttcca attgaagatt acaaaacaaa aaaaatcatc
     8881 ggtctgtcat tcggcaaaaa aggtgaggaa ctgaaacgct tccgatttaa attctgccgc
     8941 aaccacaatt ttgaagcttt aaattaccac ggcatgcctg aatatgacac ggtacaaata
     9001 aactacgcct caccagcctc ttatgatttt tttgtaaaag gctatacaaa tgatacaacg
     9061 ggcgaaacca aacccggata ctggaaacag gttattgata acgatattga tctgggcttt
     9121 acaggactgc gttttgatat tgcctacaaa attccgacac catggctgtc cgaactcatc
     9181 cagtatgccc acgaccgaca gcctgaaatc atgaccatcg cagaaacact ggccggcgta
     9241 gacgatattg gagccaagga gcttataccg aaactcgccg aagccaaaat ccttgttgat
     9301 ggggtagaaa gaccggccat cgatcacgcc atgctatcta catactgggc tggccccctt
     9361 gatggatggc ttatcgatga aagtagatta atggcgggaa tttctcgcta cggtggcgca
     9421 ggctcaccgg ataaccacga cacacaaggc acagtggccc agaacattgc caaaatgctg
     9481 gaacattatg ccccggaaga taaagagcgg gctatcgctg acatctgtgt tcgtgactac
     9541 gccttggccg ctttggtagg aaacgcccac tatgcacaaa tgggttatga agtctgcgcc
     9601 gatcaagcgg gcgtctttga agaagacaca aacccgaagc aatggcgaat gctgttggca
     9661 gaacgcgagg cctctgacca cccgctcaac atctccgaac gtctctggga aatcaacaaa
     9721 ttcaaagcat ccctgaacgc cgacaacgcc atttttcaac tcgaatctac cgccccactc
     9781 agcgaaggca gtaacttggt aaagatggtt atctcattaa cagaccagga aacaaaacag
     9841 catatgggaa ccctcgattt gtttataaac aacaaaccgg aatgcggtcc cgtagaactc
     9901 cctaacgaaa atacagcttt tatcctgacg aacagaggtg gccccaagaa ttcggacagt
     9961 gagttaagct acgatctatg accga
//
LOCUS       DAOSWR010000075         9984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4279_length_9984_cov_5.095075, whole genome shotgun sequence.
ACCESSION   DAOSWR010000075 DAOSWR010000000
VERSION     DAOSWR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4279_length_9984_cov_5.095075"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PK513_06625"
     CDS             complement(<1..987)
                     /locus_tag="PK513_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOO82158.1"
                     /translation="MAKKNYEWAEGATLDEHSKRKHKILEEYFRHYLITRCKLPQQER
                     FRLAIVDGFAGAGIYKCGRYGSPLIFIDILKKTFEEINLNRATQGMKPIEIECSLVFN
                     DLEADAIEQLKKNAAPLLAEIKQNVQQLHVDVEYYTQEFDQAFPEIKAKVLAGNYRNV
                     LFNLDQCGHSHVSINVIQDIMSSWNSAEVFLTFMISALLTYLSPDQERDRALLKMPKI
                     QKEVFALLEKGDVDSLMNKEAFLGQAEKIVFDTLKGCASSGFVSPFSINNPEGWRYWL
                     IHFANSYRARQVYNNILHDNSTLQAHFGRSGLRMLSYDPNHEGNLYLFDDNSR"
     gene            complement(1000..1755)
                     /locus_tag="PK513_06630"
     CDS             complement(1000..1755)
                     /locus_tag="PK513_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HOO82159.1"
                     /translation="MANRSKIEWTEHTWNPTTGCAKVSPGCKHCYAEAMAKRLQAMGA
                     NGYDNGFKLSIMEERLNQPLKRKKGTTYFVNSMSDLFHEGIDDVFLDRVFGVIEKTPQ
                     HQYQILTKRAERLPEYFGEHKVPQNVWLGVSIEDKKYGVPRIDLLRQVNAYIRFLSVE
                     PLLEDIGDIDLTNIHWVIVGGESGPKARPMKPEWVENVRIQCEEQGAAFFFKQWGCWG
                     ADGKKRSKAENGRELNGRTWDEMPSAVAEQVWR"
     gene            2187..4436
                     /gene="mobQ"
                     /locus_tag="PK513_06635"
     CDS             2187..4436
                     /gene="mobQ"
                     /locus_tag="PK513_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HOO82160.1"
                     /translation="MAIYHCSLRVFSRSKGHSAVAAAAYRSGSVLYDERAETTHRYDK
                     RSGVAETFIVLPEHAPFDPADRAAVWNAAEASETRKNSCVARELVLALPHELSGADRH
                     SLTRDMASWLKERYRVAVDAAIHIPVKGDDARNHHAHLLFTTREVTNTGMGKKTRILD
                     DKVTGKEETELIRQVWETLANDALKSAGFTDVKIDRRTLQEQGIERIPQTHIGPQAQK
                     AVKSASDDEDEQEGKGSAKGSAGGAAQGPKKNKDKDEQGDSEQGEQGGSGDPVALKLK
                     SKVKTDNQGRIIDYRVIDRRQTRQNFVAEIKALNEKRAAFGDAPLKDQIEGLDRLMEK
                     LDVRLERLQTIEHKTALPARIVELFDKAMQKAVQQLGLRKDERLQTSLSRTEEKARHE
                     RQHARYGRTYRKGLHEQIREMKASIERLEYKHKEYERYKGFVDTLEQAMLKRVPAVTP
                     DITKDPAKPVPKTSNAESTLKLQLKASVMRENLPLADTPQVQVKRPEGVARATSPEHV
                     STDINTPPSKEPLKLSEITRERFAPELKAKPVQKQFNSVAEELEAERARAEDYKQKNT
                     VVFAALKQEIENRTVALQVKDEKPSPSYRQPMKVEIKALKEAMQERAQNPIRTQEQIT
                     AARQDQKTETKTQFNQAERPAPKIVTVEELTTRMQAEAAKLRTKVPEQYRAESYEERQ
                     APPSSVKTSFSEAQTENPKNTTVQPVPSPAIKMSARFNSASAQDISEHPVPYETPEAP
                     HDDIDLDIE"
     gene            4694..5203
                     /locus_tag="PK513_06640"
     CDS             4694..5203
                     /locus_tag="PK513_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82161.1"
                     /translation="MSGISKSVFHAVLLTVIIMLIGGAFYIYADSENFWEVTDPLDPQ
                     FNPDDFAFNNYDNHDELAEVLKILIHPGMSRKEAENFLDGSPDKRGGNDRTNDEHILT
                     GYTIRQKIDIEMLRAKSSKVVSYGFGGRRKLMNPLFGGWHVTAFYDEQDRIIQLVVRN
                     EPLFDEGRL"
     gene            5203..9933
                     /locus_tag="PK513_06645"
     CDS             5203..9933
                     /locus_tag="PK513_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOO82162.1"
                     /translation="MSTYYLGWSDILWNNNSGLDKHIYLVKDLDGDLQSTNDQSFIRG
                     TAENNDITDFLFGGTGSLLVEDGLLSSESRDGLDNINDSNLDGYLDNDKNKDGVADNY
                     VDRHYTQVTITDSQWNAMLQLAADIDAAGYEYGLLSANCQAIVVSMLSLAGLDFEQNV
                     PIASSYTDYVSRNVLLSGAGSDTLYGFDHDDRIFDRGGGDDDFYGGDATNPVRFDDSD
                     GMDAVHYDDEDETGTLKIYDTYRIVQEGNGSTDVLYSIEKVGFEGFDESNTLDYSNYS
                     SSVDIDSFSFVGRLAEPGDVRTAVRVKETPGGFLGFGAEVFEAINFGYFKLTDYDDKI
                     CLKSEGVAEAVTVDAGAGDDEIDVSVTGATIFAGAGEDTISRAINGTVIYTGAGDGDK
                     DVINIFYDNVLIADAGTEDRIHFLGNELTGGFKMHISESSWTQWTVDGVRFGINSEGQ
                     LGIQTLLGKTTYVDNFQYTLDGIENDTAGIIVFELDIEAYRVLELPKGWLTSTLKAFH
                     NLMELVFADYDAGPSDPLVLDLDGDGLEVAPNTVVSPKFDLNGDGFAERTGWTLNGDD
                     GFVVRDINSDGVINDISEMFGNDTTSGFAALAAFDTNTDGVVDQAEATAAGIQIWKDA
                     DRDAQTDAGELVSFSDAGVSSITVTPTNTNPDNSANTGFAFLSAGEFTRTDSSTGAAY
                     DVVFGANEYDSEWLEDVTLTAGALALPEVKGHGTLPSLRAAMSYDSGFETIVSNTLTA
                     LNTPDVDVLRSTVMPILNGWAASVSVPAGHPGTEARIDVPVLVNTAGGGDGVEVVDFG
                     VQREDAQGTYWVLASGDDVLDAQSQVIARPTYQDILDQAPGTDEAWQVVTAEQIQFME
                     RWLGQSMPLSMDTNAGASAVNGMSGLLDIIVNELNSVAIRIASQDGPLASFFDGVEYN
                     HETDRFESTTDRQLAPMLEAIFAAAPGNAAGDAAHLDAWNGVLDIVIDQYDRDGDSSA
                     NTYSFLFQNVVMAYENIPLAVDIVTAAAALDIPEDLIITGSGTLNGTTDADIFYMDGS
                     NQTVNGDEGPDSFVFGKNIGHDVIAEVDPSYGEDLDDTVRFTAHNASDMTFVRDDKDM
                     VITITETGESIRLVDQFEGRVAALTPAYEDYATGVSLIIFANGEVWDRVDMAYAVAHP
                     NAGDDHIQESNAINVLDGGAGNDLLEGGNQGDIYIFGQGYGQDFINDYRTDFLTTTPD
                     FVKFNAGITQSDLIFTREGKSDDVTITIDGTTDTLTIKDQFWASYLGSDPEWFNRIEG
                     FSFEDGSMMLWSDVIELIPQTLKTSGDDTIYGFSYEDTLDGGTGNDYLSGGDENDTYI
                     YGLGYGHDTIEEDVDNFLSGADDQVVFLADIDPGTVTFSRTADSDDLVFTFADSGTLT
                     IIDQFKYWNYVPFGPKGLQRIESFVFNDPAQTTWTYDYIQSELIDLSQTTGNDTIYGY
                     DGYEDVLEGGLGDDHILGGYEGDTYIYNSGDGNDIIEDNHQNQSRIPVGANSDKLVFT
                     DIASTNVALEWGDGEWDLKIRVLSTDETINIVDQNKRFATGSLYYTIEEVEFSDSVTW
                     TSEQMRDLYLAGAATSGDDYIHGF"
BASE COUNT         2655 a         2189 c         2552 g         2588 t
ORIGIN      
        1 gcgtgaattg tcatcgaaaa ggtacaaatt cccttcatga tttggatcat aggaaagcat
       61 acgtagcccg gagcgtccaa aatgggcttg aagcgtactg ttgtcgtgca agatattgtt
      121 atatacctgc cgggcgcgat aactgttggc aaaatggatc aaccaataac gccagccttc
      181 tggattgttg attgagaagg ggctgacaaa acctgaagaa gcgcacccct tcagagtatc
      241 aaatacgatc ttttccgctt ggcctaagaa tgcttctttg ttcatgaggc tatctacgtc
      301 acctttttcc aaaagcgcga acacttcttt ctggattttc ggcatcttta agagcgctct
      361 gtcccgttcc tgatctggtg aaaggtacgt tagcagggcg gaaatcatga aagtcagaaa
      421 aacttcagcc gagttccatg aagacatgat gtcttggata acattaatgc tcacatgcga
      481 gtggccgcac tggtccagat taaacagcac attccggtaa tttccggcca acactttagc
      541 cttaatttct ggaaacgctt ggtcaaattc ctgagtgtaa tattcaacat caacatgaag
      601 ctgctgaaca ttctgcttta tctccgccaa taacggcgcg gcatttttct tgagttgttc
      661 aatcgcgtcc gcctcaagat cattaaagac aagggagcat tcgatttcga ttggcttcat
      721 gccctgtgta gcacgattaa ggttgatctc ttcaaaagtc tttttcaaaa tatcgatgaa
      781 gatcagggga gaaccatagc ggccacattt gtaaattcct gcgcctgcaa aaccatcaac
      841 gatggccaac ctaaatcgtt cttgttgggg aagcttgcaa cgggttatca agtaatgcct
      901 gaaatactcc tcgagtattt tgtgtttccg tttgctgtgt tcatcaagtg ttgcgccttc
      961 agcccattca tagttctttt ttgccattat actacaatcc taacgccaca cttgctctgc
     1021 aactgcactc ggcatttcgt cccatgtcct gccgttcaat tcacggccat tctctgcctt
     1081 agaccttttt ttgccgtcag cgccccagca tccccattgc ttaaagaaaa acgccgcgcc
     1141 ttgttcttca cattgaatgc ggacgttttc aacccactct ggtttcattg gtctcgcctt
     1201 cgggccgctt tcgccgccaa caatgaccca gtgaatattt gtcaggtcaa tatctcctat
     1261 atcttcaagt agcggctcta ctgataaaaa gcggatatac gcattgacct gccgcagcaa
     1321 atcaatccgt ggcacgccgt acttcttatc ttcaatggaa acacccaacc aaacattttg
     1381 tggaacctta tgttcgccaa aatattccgg caaacgctct gcgcgttttg ttaaaatctg
     1441 gtattgatgt tgcggtgtct tctcaatcac accaaatacc cggtcaagaa acacatcatc
     1501 aatgccttca tgaaacaaat cactcatgga attgacgaag taggttgtgc cctttttgcg
     1561 ttttaatggc tgatttaggc gctcttccat gattgaaagt ttaaagccat tatcgtagcc
     1621 gtttgccccc attgcttgca gccttttggc catagcttca gcgtaacagt gcttgcatcc
     1681 gggtgatact tttgcacatc cggttgtcgg attccatgtg tgttctgtcc attcaatctt
     1741 acttctattt gccatatctg tatccaaagc ctaagaacaa aacataaaca tacgttacca
     1801 tagagttcat aaagtgcaaa cctttacggt agggaaaact aaatcctata gctagaatga
     1861 tagtgagatt ttaggtatag cctagtaatg aaaacaaata aatccctgat ttataagccc
     1921 tcatgcttcc tttgagcatg atctctatct ccgtccgggt gggggacgtg tggctggttt
     1981 ggtggtaatc ttaacgaaaa cctaatctga attgatatat cctaaaaaga cgaatagcgc
     2041 acttatgcaa actaaagttt gcgtgcgcca ccagcggccc ggcagtgaag aaatcgcctg
     2101 acacctgctg cgtcgtttta gctcaagctt gcggagctaa acgtaagcag gtgtgtttac
     2161 tgcgtaaaaa gggaggtgta acctccatgg cgatttatca ctgttccttg cgagtatttt
     2221 cacgaagcaa aggccactca gccgtggccg cagcggcgta tcgttccggc tccgtgctgt
     2281 atgatgagcg agccgaaaca acccaccgct atgataaacg ctccggcgta gccgagacgt
     2341 ttatcgtgct tcctgaacat gcccccttcg atcctgcgga tagggccgct gtgtggaatg
     2401 cggcagaagc cagtgagacg cgtaagaatt cctgcgtagc gcgagagctg gttctggcct
     2461 tgccgcatga actgtcgggg gccgacaggc actctttaac ccgtgatatg gcgagctggc
     2521 ttaaagagcg ctaccgcgtt gcggttgatg cggccattca tatccctgtg aaaggggatg
     2581 atgcccgcaa tcaccatgcc cacttgctct tcacgacgcg ggaagtcaca aacacgggca
     2641 tgggtaaaaa gacccgtatc ctcgatgaca aggtaacggg caaagaagaa accgagctta
     2701 ttcgccaggt ttgggagaca ctggccaatg acgctctgaa aagtgctggc tttacggatg
     2761 taaagatcga ccgccggacg ctgcaagagc agggcattga acgtatcccg caaactcata
     2821 tcggcccgca ggcgcaaaag gctgttaaaa gcgcatccga tgatgaggat gagcaagaag
     2881 gtaagggcag cgctaaaggt tcggctggtg gggctgcgca aggcccaaag aagaacaagg
     2941 ataaagacga acaaggtgat agcgagcagg gggagcaggg cggttctggc gatccagtgg
     3001 ctttaaagct caaaagcaaa gtaaagaccg ataatcaagg ccgcattatt gattaccgcg
     3061 ttattgaccg ccgtcaaact cgtcagaatt ttgttgccga gatcaaagcc ctcaacgaaa
     3121 aacgcgcagc ctttggtgat gcgccgctca aagaccagat cgagggcctt gatcggctca
     3181 tggaaaagct cgacgtgcgg ttggaacgat tgcaaaccat cgagcataaa accgcgctgc
     3241 ctgcgcgcat tgttgaactg tttgacaaag ccatgcaaaa ggctgtccag cagttgggct
     3301 tgcgaaaaga tgagagactt caaaccagcc tatcacgcac tgaagagaag gcccggcatg
     3361 agcgccagca tgcgcgttat ggccgcacct accgcaaagg tctacacgaa caaatccgtg
     3421 aaatgaaagc cagcattgaa cgccttgaat ataagcacaa ggaatatgag cgctataaag
     3481 gctttgtcga cacgctcgaa caggcaatgc tcaaacgcgt ccctgcggtt acgccagaca
     3541 ttacaaaaga cccagctaag cccgttccca agacctctaa tgccgaaagc acgctgaagc
     3601 tccagcttaa agccagcgtg atgcgtgaga atttaccgct tgctgacacc cctcaggttc
     3661 aggtgaaaag acctgaaggg gtggcgaggg cgaccagtcc tgaacatgtc tctacagaca
     3721 tcaatacccc accatcaaaa gagcctctca aactcagcga aatcacgcgc gagcgctttg
     3781 ctccagagct taaagccaag ccggtgcaaa aacagtttaa ttctgtggcc gaagagctgg
     3841 aggccgaacg ggccagggct gaagattata aacaaaagaa tacggttgtg ttcgccgcgc
     3901 taaaacagga aatcgaaaac cgcacggtag ccctgcaggt gaaagatgaa aaaccgtccc
     3961 cgtcataccg tcagcccatg aaagttgaaa tcaaagcgct gaaagaggca atgcaggaac
     4021 gggcgcaaaa cccaatccgg acgcaagagc aaatcaccgc cgcgcgtcag gaccaaaaga
     4081 ccgaaacaaa aacccagttt aatcaggcgg aacgtccggc ccctaaaatc gtcacggtcg
     4141 aagaactgac cacgcgtatg caagctgaag ctgccaagct caggacaaaa gtgccggagc
     4201 aatatcgcgc tgagagttat gaagaacgcc aagccccacc atccagcgtg aagacgagtt
     4261 tctcggaagc acagactgaa aacccgaaaa acactactgt ccagccagtc ccatcacctg
     4321 cgatcaaaat gagcgcacgc ttcaacagtg cctctgctca agacatttca gaacaccccg
     4381 tcccctacga aacgccagag gcaccccacg acgatatcga cctcgatatc gaataatccc
     4441 taaaaatttt catgaattaa attgtgtaaa taaatacacc aataaagcgt atttgttttg
     4501 cctgtaaacg caaaggttag actcaataaa aaaaaatctt ttacaagatt gttttttatt
     4561 tttctgcttg gcacgatcta gtcacaaaaa acacaaatga caatagggat ggattcagct
     4621 ttgcaattca caatggccaa aagacctgct ccaatacact ccggtatcac catagataaa
     4681 aggagtgatt gagatgtccg gtatttctaa aagtgttttt catgctgtct tgctaactgt
     4741 aatcattatg ttaattggag gtgctttcta catttatgcg gatagtgaaa atttttggga
     4801 ggtcactgat cctctggacc cccaatttaa tccagatgat tttgccttta acaattatga
     4861 taaccacgat gagttggccg aagttctaaa aatattgatt catcctggta tgtctcgaaa
     4921 agaagctgaa aattttttgg atgggtcccc tgataaaaga ggcggcaatg ataggacaaa
     4981 tgatgagcac atacttaccg gttatacgat ccgacaaaaa atagatattg aaatgctcag
     5041 agcaaaatct agcaaggttg tctcttatgg gtttggtggt aggcgtaaac ttatgaatcc
     5101 actttttggg ggatggcatg ttacggcttt ttatgatgaa caagaccgta tcatacagct
     5161 tgttgtacgg aacgagccat tattcgatga agggagatta taatgtcgac atattatcta
     5221 ggttggtcag atatactttg gaataataat agtgggcttg ataagcacat ttacctggta
     5281 aaggatttag atggggatct acagagtaca aacgatcagt cctttattcg tggcacagct
     5341 gagaataatg atattactga ttttctgttt ggtggtacgg gttctttatt ggttgaggat
     5401 ggcttgttat caagcgagtc gagagacgga cttgataata tcaatgatag taacctagat
     5461 ggttatctag ataatgataa gaacaaagat ggcgttgcag ataattatgt tgataggcac
     5521 tatacacagg taactattac agattcgcag tggaacgcta tgctacaact ggctgcggat
     5581 attgatgcgg ctggctatga atacggtttg ctgagtgcta attgccaagc tattgttgtg
     5641 tcgatgttgt ctctcgctgg cttggatttt gagcagaatg tccctattgc cagctcttat
     5701 actgattatg ttagtcgcaa tgttttgctg agcggcgccg gttctgacac cctctatggt
     5761 tttgatcacg acgatagaat ttttgatcgc ggcgggggtg atgatgattt ttatggtggt
     5821 gatgcgacta atccagttcg gtttgatgat tcagatggca tggatgcggt tcattatgat
     5881 gatgaagatg aaaccgggac gctgaaaatt tatgatacat accgtattgt tcaggagggg
     5941 aatggtagta cggatgttct ctattccatt gaaaaagttg gttttgaagg attcgatgag
     6001 tctaatacgc tggattactc aaattacagt tcctccgttg atattgacag ttttagcttt
     6061 gtgggccgtt tggcagagcc gggggatgta cgcacggctg taagagtgaa agaaacaccc
     6121 ggtggctttt taggttttgg tgcggaagtt tttgaggcaa tcaactttgg ctatttcaag
     6181 ctcacggatt atgacgataa aatttgttta aaaagtgaag gcgttgcgga ggcggtaacc
     6241 gttgatgcgg gcgcgggcga cgatgaaatt gatgtcagtg ttaccggtgc aacgattttt
     6301 gccggggcgg gtgaagatac gatcagccgt gcgatcaatg gaaccgtgat ttataccggg
     6361 gcgggtgacg gtgacaaaga tgtcattaat atattctatg acaatgttct tatcgccgat
     6421 gcgggcacgg aagaccggat tcattttttg gggaatgagc taaccggcgg atttaagatg
     6481 cacatttctg aaagttcttg gacgcaatgg actgtcgatg gtgtgcgctt tggtattaac
     6541 agcgaagggc aacttggtat ccaaaccctt ttgggcaaaa caacctatgt cgataacttc
     6601 caatacacgc tcgacgggat agaaaacgat accgccggga ttatcgtttt tgagttggat
     6661 atagaggctt atcgtgttct ggagctgccg aaagggtggc tgacaagcac gttaaaggct
     6721 ttccacaatc tgatggaatt ggtttttgcc gattacgacg ccgggccgag cgacccgctg
     6781 gtccttgatc tggacgggga cggtctggag gttgcgccga acacggtcgt gtctccgaaa
     6841 ttcgatctga acggggacgg atttgccgaa cgcacaggct ggacgctgaa cggcgatgac
     6901 gggtttgtcg tccgcgatat taatagtgat ggcgtcatta atgatatctc ggaaatgttt
     6961 ggtaatgata ccaccagtgg ctttgctgcg ctggctgctt ttgatacgaa tactgacggc
     7021 gttgtcgatc aggctgaggc cacggcggct ggcattcaaa tctggaaaga tgccgaccgc
     7081 gatgcgcaaa ccgatgcggg tgagttggtc agctttagcg atgcgggcgt gagttcaatc
     7141 actgtcacgc cgaccaatac caatccagat aattccgcca ataccggttt tgcgttttta
     7201 tccgcagggg agtttacgcg cacagatagc tcaactggcg cggcctatga cgtggttttc
     7261 ggcgcgaatg aatatgacag tgaatggctg gaggatgtta cgcttacagc tggggcattg
     7321 gctttgcctg aggttaaagg gcatggcacg ttgccgagtt tacgcgcggc gatgagctat
     7381 gatagtggct ttgaaacaat cgtgagcaat acgttgacgg cgctcaatac gccggatgtt
     7441 gacgtattgc gcagcactgt gatgccgatc ttgaatggct gggccgccag tgtgagtgta
     7501 cctgccgggc atccgggtac ggaagcgcgg attgatgtgc cggtgctggt gaatacggcc
     7561 gggggcggcg acggtgttga ggtcgtcgat ttcggtgttc agcgcgagga cgctcagggc
     7621 acatattggg ttctggccag tggtgatgac gtgctggatg cgcaaagtca ggtcattgcc
     7681 cgcccgacct atcaggatat tctggatcag gcaccgggaa cggatgaagc atggcaggtg
     7741 gttacagcgg agcagatcca gtttatggag cgttggcttg gccagagtat gccgctgagc
     7801 atggacacaa atgcaggcgc tagcgctgtg aatggtatga gcggcctgct ggatattatt
     7861 gtgaacgaat tgaactcagt cgctatccgc attgcctctc aggacggacc gctggcgagc
     7921 ttttttgatg gcgtggaata taaccatgag acggatcggt ttgaaagtac aactgacagg
     7981 cagcttgcgc caatgctcga agcgattttt gctgctgcgc cgggcaacgc tgctggggat
     8041 gctgcgcacc ttgatgcgtg gaatggtgtt ttggatatcg taatcgacca gtatgatcgc
     8101 gacggtgata gttctgccaa tacgtattcc ttcctgtttc agaatgttgt gatggcctat
     8161 gagaatattc cactggctgt tgatattgtc acggctgctg ctgcgcttga tattcctgag
     8221 gatttgatca taaccggttc agggacatta aacggtacaa cggatgctga tattttctat
     8281 atggatggca gcaatcagac tgtaaatggc gacgaaggcc cggatagttt cgtgttcggc
     8341 aaaaatatcg gccatgacgt aatcgctgag gtcgatccgt cctatggtga agatcttgat
     8401 gataccgtgc gttttaccgc tcacaacgca tcggacatga catttgtgcg tgacgataag
     8461 gacatggtta tcaccattac tgaaactggg gaaagtattc gtcttgttga tcagtttgaa
     8521 gggcgcgtgg ctgcattgac cccagcttat gaagattatg caacaggtgt atcactgatt
     8581 atctttgcta atggtgaagt ttgggatcgt gtcgatatgg cctatgcggt cgcacaccct
     8641 aacgctggtg acgatcatat tcaggaatct aatgcaataa atgtactcga tggtggtgct
     8701 ggtaatgacc ttctggaagg tggaaatcag ggcgatatct atatttttgg tcaaggttat
     8761 ggtcaggact tcattaacga ttaccgaacg gattttctaa caactacccc cgattttgta
     8821 aaatttaatg ctggaattac tcaaagcgat ttgatcttta cgcgtgaagg gaagtccgat
     8881 gacgtaacca tcacaattga tggcacaaca gacacgctca ctattaaaga ccagttttgg
     8941 gctagttatc ttggctctga tccggaatgg tttaaccgca tagaagggtt ctctttcgaa
     9001 gacggtagca tgatgctgtg gagtgatgtg attgagctta ttccgcaaac gctaaaaaca
     9061 tctggcgatg atacgattta tggcttttcc tatgaagata cactcgatgg cggcacgggt
     9121 aatgattatc tctcaggcgg tgatgaaaat gacacctata tttacggtct ggggtatggt
     9181 catgacacga ttgaagaaga cgttgataat tttttaagcg gcgcggatga ccaagttgtt
     9241 ttccttgcag atatagatcc gggcactgtg actttctcgc ggactgcgga ttccgatgat
     9301 ctggttttca cctttgcgga tagtggaaca ctcaccatca tcgaccagtt taaatattgg
     9361 aattacgtac cctttgggcc gaaaggtttg caacggattg agagctttgt attcaacgat
     9421 cctgcgcaaa ccacatggac atatgattat atacagtccg agttgatcga tttaagccaa
     9481 acaaccggca acgatacgat ctatgggtat gatggctatg aagatgtctt ggaaggtggc
     9541 ttgggcgatg atcatattct cggaggctat gaaggcgata cctacatata taatagtggc
     9601 gatggcaatg atatcatcga agacaaccac caaaatcaat cacgtattcc agtaggagct
     9661 aattcagaca agcttgtctt tacagatata gcgtctacaa acgtggctct tgaatggggc
     9721 gatggtgaat gggacttgaa aatccgggtt ctgagcacag atgaaacgat caatattgtt
     9781 gatcagaata agcgctttgc aactggaagc ctgtattaca ctattgagga agtcgagttt
     9841 agtgacagcg ttacatggac gtcagagcaa atgcgtgacc tttatttggc aggtgctgca
     9901 acgtccggtg atgactatat tcatggcttt tagaggtggt ctcggttctt cggccagctt
     9961 tgcggcgaag ttaagctacg atcg
//
LOCUS       DAOSWR010000076         9814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4385_length_9814_cov_6.050825, whole genome shotgun sequence.
ACCESSION   DAOSWR010000076 DAOSWR010000000
VERSION     DAOSWR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_9814_cov_6.050825"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="mnmG"
                     /locus_tag="PK513_06650"
     CDS             complement(<1..1331)
                     /gene="mnmG"
                     /locus_tag="PK513_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOO82163.1"
                     /translation="MDMDYDVIVVGGGHAGCEAAAASARMGAKTALLTHKLSTIGVMS
                     CNPAIGGLGKGHLVREIDALDGLMGRIIDQAGIQFRMLNASKGPAVRGPRAQADRKLY
                     REAMQAELSDYPNLTLVEGAAEDLILDETGEVVGVKAQDGREFKCAAVVLTTGTFLRG
                     MIHRGEEQTPAGRVGEGSSIGLAETLERLEFPLGRLKTGTPARLDGRTIDWDKCEEQL
                     GDTDPRPFSAMNDEIAVPQISCHITYTTEETHKIIAENIHRSAMYSGNISGVGPRYCP
                     SIEDKINRFADKPRHQVFLEPEGLDDHTVYPNGISTSLPVEVQDAYIHSIPGLENVKV
                     LEWGYAIEYDYCDPRDLKATLESKRLPGLFLAGQINGTTGYEEAGAQGLMAGLNAALQ
                     AKFMHESVGKAVDDSRETLRAYATVFTLDRADAYIGVLIDDLITRGAPEPYR"
     gene            complement(1370..1801)
                     /locus_tag="PK513_06655"
     CDS             complement(1370..1801)
                     /locus_tag="PK513_06655"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOO82164.1"
                     /translation="MPGEMSLEKGQYYAHPRNQFWLIIKQVFEVSQDLEYCESLALLK
                     DRHIALWDVLKSCRRTGSLDSAIQEEKANDFITFFKAYPSIKTVCFNGKKAGASFRKH
                     VFPVLDIDRFSFHTMPSTSPAYAGLSFEGKYQAWREVLKVK"
     gene            complement(1870..>2356)
                     /locus_tag="PK513_06660"
     CDS             complement(1870..>2356)
                     /locus_tag="PK513_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOO82165.1"
                     /translation="QEGIESEGIRRALLRAKTADITLLVFDANAPEVDAHTLALIDER
                     AVLLENKTDKDGAKAQIEAALPISVETGEGLDALLGLLQEKIEGLIGRREAPALTRRR
                     HRGCIEEAREALVRAQTAALPELMAEDVRLAVRALGRITGRVDVEDLLDVIFRDFCIG
                     K"
     assembly_gap    2357..2366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2367..3215)
                     /gene="mnmE"
                     /locus_tag="PK513_06665"
     CDS             complement(<2367..3215)
                     /gene="mnmE"
                     /locus_tag="PK513_06665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOO82166.1"
                     /translation="MSETIFALASGPGRAGVAVIRVSGPQSWAGLQALSGRADFIPRK
                     AALVDLKNEDSRETLDRALVLPFKAPASYTGEDVVEYHLHGGRAVVDAVLAALAGLDG
                     HRMAEPGEFTRRAFENGKMDLTEAEAVADLIDAETQAQRLQALLQMGGVLMNLYEGWA
                     DRLKRALAHLEAEIEFPDEDLPQEIAPEITADLSAISQEMSEHLNDNRRGERLRDGIH
                     VAVIGAPNVGKSSLVNVLAQRDVAIISEMAGTTRDVIEVHLDLGGYPVILADTAGLRP
                     DQIGAEG"
     gene            complement(3216..3461)
                     /locus_tag="PK513_06670"
     CDS             complement(3216..3461)
                     /locus_tag="PK513_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HOO82167.1"
                     /translation="MKVTFDIDCTPEEARKFLGLPDVAPLQEAMMEELQAKMQENVQA
                     MDPEAMMKTWFPAALQNMSDMQKMFWGQMGLKTGEPE"
     gene            3538..5076
                     /locus_tag="PK513_06675"
     CDS             3538..5076
                     /locus_tag="PK513_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82168.1"
                     /translation="MNDEPPYPQNPPADQPKTGSGSEALNTRIYHSEIDPELKTKALL
                     QMCEAIKGNGLKNEDVDITAYDGETLEFLAITEMKLDTKLSIKRQAGKITGPTLVANA
                     GAFQEAVNKETYKITHSLDVVERIRDAVLERPDKGLCLKNILIKLPFLHKDFVTHEPC
                     SACASKGKITCVRCHGKGREPCPRCHARGSETCPTCGGRQYILGPNNQNQQCMRCNGT
                     GRIGCSQCHESREVQCAMCKAVGTMQCRQCNGHAWNSVLCRAEINPVGLYSFDRTTIP
                     QQALEKLDLLGSDIQHHSNIQIIHRHKELEQERNDISIPYHIKVPMAHVTFMLKEKLE
                     IPAFLFGTQAKLYDMPPFLEKITAPGIKALKAAAMGQGDAASLIQKAGRYRTLREILL
                     ATSKLGPKKTLKVIEEKNPIGLSEDTTRKLVLMACKAIGFLGKKPRQISIALGLLITA
                     GLGAFYWYIGKAALLPYLPHTNNIALLTDGIAGLTALGAGYVTAKTYLKRAMQKSLES
                     LFSK"
     gene            complement(5238..5753)
                     /locus_tag="PK513_06680"
     CDS             complement(5238..5753)
                     /locus_tag="PK513_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82169.1"
                     /translation="MKTQKYSNCWKTDPRAAYIPGAVLVSLVLLLCLTVGPAWSFETL
                     DSVQDQTAFDDPKQALTTSYPSQPSSPTVADSCLSLLKSIHHTSPVSVTDRNQRSAGK
                     AAALGLVLGVRFALSPPQKAKSRADRPRLDVWQTSGTFAGDRSALAVTAYRQCQKEQA
                     LKAISEFRWAR"
     gene            6084..6494
                     /locus_tag="PK513_06685"
     CDS             6084..6494
                     /locus_tag="PK513_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO82170.1"
                     /translation="MHIADMPEFKNRKQVLTCKPDDTVYDVVVMMAFKHFGAVAVTDN
                     DKLVGIFTERDLLVRVVGQGRDVESTKISDVMSKGIETATAHDSALLCMGRMNHGRFR
                     HMPVTDNNGKLTGMLSQRDFIAFTMRELKEKLAD"
     gene            complement(6513..7769)
                     /gene="rho"
                     /locus_tag="PK513_06690"
     CDS             complement(6513..7769)
                     /gene="rho"
                     /locus_tag="PK513_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO82171.1"
                     /translation="MNLQELKTRSPAELLAFAEELEIENCSAMRKQDMMFAILKQLAT
                     QGTPIFGAGVLEVLQDGFGFLRSPEENYLPGPDDIYVSPSQIRRFGLRTGDTVEGEIR
                     APKDSERYFALLKVGSINNETPDKLRHRINFDNLTPLYPERKIKLELDDPTKKDNVQR
                     VIELTSPIGFGQRALIVAPPRTGKTVMLQNIATSIAENHPDAYLIVLLIDERPEEVTD
                     MARSVRGEVVSSTFDEPASRHVQVAEMVMEKAKRLTEHKRDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKISDKRVFPAIDIQKSGTRKEELLVDRDTLQKMWVLRRILTPMGT
                     VDAVEFLLGKMKQTKNNDEFFNAMNQ"
     gene            complement(8234..8683)
                     /gene="hemJ"
                     /locus_tag="PK513_06695"
     CDS             complement(8234..8683)
                     /gene="hemJ"
                     /locus_tag="PK513_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HOO82172.1"
                     /translation="MQDFLLQHYEWVRALHIISVIAWMAGMLYLPRLFVYHTAVVAGS
                     EMSETFKVMERRLLKVIINPAMIVSWLFGLSMLYANPGMLEGQGWMHAKLLFVVLMSA
                     VHGMLAKDVKRFAHDENTKSSKYYRILNEVPTVLMIGIVIFAVVQPF"
     gene            complement(8688..9776)
                     /gene="hemH"
                     /locus_tag="PK513_06700"
     CDS             complement(8688..9776)
                     /gene="hemH"
                     /locus_tag="PK513_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOO82173.1"
                     /translation="MAEKIAVVLFNLGGPDSKNAIRPFLLNFFMDKNIISLPVPFRCL
                     LATMIANRRSKREAGESYGELGDKSPLLDNTNAQAKALEKVLKADSRETKFKVFTCMR
                     YWHPMAAQVVREVRDWGADKIILLPLYPQFSTTTTWSSLEQWNKALFEAGIEIPSAMV
                     CCYHENAGFVSASAENIKACYDQALKDGHEAPRVLFSAHGLPEKIIKDGDPYQWQCEQ
                     SAKAIVKKLGIEGLDYQYCYQSRVGPQKWIGPSLGEALVKAAQDKRAVIVYPHAFTQE
                     HVETLVELDVEYKEEAQKLGVSGYYRAQTVGTHPDFIEGLAGLVEEYLDKDGVAPEGF
                     KCPCSEEFGKCCVKTRAVMVCSICKEAA"
BASE COUNT         2685 a         2879 c         1931 g         2309 t
ORIGIN      
        1 cggtacggct ccggcgcacc gcgcgtaatc aaatcatcaa tcaacacccc gatataggca
       61 tcggcccgat ccagcgtaaa aaccgtcgca tacgctctca atgtttcacg tgaatcatcc
      121 acagctttac ccacagactc gtgcataaat ttcgcctgca acgctgcatt cagtccggcc
      181 atcaaccctt gcgcgccagc ctcttcatag ccagtcgtcc cgttaatctg cccagccaga
      241 aacaggcccg gcaatcgctt gctttccagc gttgccttca aatcccgcgg atcgcaataa
      301 tcatattcaa tcgcataacc ccactcgagc accttgacat tttcaagacc cggtatcgaa
      361 tgaatatagg catcctgcac ctcaacaggc agggacgtcg aaatcccgtt cggatacacc
      421 gtatgatcat ccagcccctc cggctccaga aaaacctgat gacgcggttt atcggcaaaa
      481 cgattaatct tgtcctcaat cgaggggcaa tagcgtggcc ccacaccgga aatattaccc
      541 gaatacatgg cactgcgatg gatattctca gcaataatct tatgcgtttc ttccgtggta
      601 taagtaatat gacaggaaat ctgtggcact gcgatttcat cattcatagc ggaaaacgga
      661 cgcggatcgg tatccccaag ctgctcttca catttgtccc aatcaatcgt acgtccatcc
      721 agccgggcag gggtcccggt cttcaaacgc cctaaaggaa attctagccg ttccagcgtt
      781 tcagccaacc cgatagacga cccttcacca acacgccccg ccggcgtttg ctcctcaccc
      841 cggtggatca ttccacgcaa aaatgtcccg gtcgtcagca ccacagccgc acatttaaac
      901 tcacgcccat cctgcgcttt caccccgaca acttcaccag tctcgtcaag aataagatcc
      961 tcagccgcgc cttcaaccag cgttaaattt gggtagtccg ataattccgc ctgcatcgcc
     1021 tcacgataca gcttacgatc cgcctgcgca cgcgggccgc gcaccgccgg gcctttggaa
     1081 gcattgagca tccgaaactg aatgcccgct tggtcgataa ttcgccccat caacccgtcc
     1141 agcgcatcga tttcacgcac cagatgtccc ttgcccaatc cgccaatcgc cggattacaa
     1201 gacatcacgc caattgttga aagcttatgc gtaagcaaag ccgtcttcgc acccatacgc
     1261 gcactggccg ccgccgcctc acatccggcg tggcctccac caacaacaat cacgtcataa
     1321 tccatatcca tggccgccct tatacaggaa aagccagcca gaatgccagc tatttcacct
     1381 tcagaacctc tcgccaagcc tgatatttgc cctcaaaaga caatcctgca tatgccggac
     1441 tggtagacgg catcgtgtga aatgaaaacc gatcgatatc aagaaccgga aaaacgtgct
     1501 ttcgaaagct ggccccagct ttcttaccgt taaaacaaac tgtttttata ctgggataag
     1561 ccttgaaaaa agtaataaaa tcatttgctt tctcttcctg aatcgcactg tcaagactac
     1621 cagtccgcct gcaacttttt agcacatccc acaatgcaat atgccgatct ttcaaaagcg
     1681 caagagattc acaatactca agatcctgcg atacctcaaa aacctgctta atgatcagcc
     1741 agaactgatt gcgcggatgt gcataatatt gccctttttc caaagacatt tcaccaggca
     1801 tactgccaag aacaagcaac cgcgcatcag aatttgccac tggcgaaaaa cttgaaatcg
     1861 tactcatctc tattttccta tgcagaaatc gcggaaaatc acgtctaaca aatcttcaac
     1921 atccacccgg cccgtaatcc gccccagcgc ccgcacagcc aagcgcacat cttccgccat
     1981 cagctccggc aaagccgccg tctgcgcgcg caccaacgct tcacgcgctt cctcaataca
     2041 gccgcgatga cggcggcggg tcagggcagg ggcctcacgc cgcccaatca aaccctcaat
     2101 cttttcctgc aacaagccca ataacgcatc caacccttcc ccggtctcta ccgaaatcgg
     2161 caacgccgcc tctatttgcg ccttcgcccc gtccttatcc gtcttatttt ccaacaacac
     2221 cgcccgctca tcaatcaggg ccaatgtatg cgcatcgacc tctggcgcat ttgcatcaaa
     2281 caccagcaat gtaatatcgg cggttttcgc ccgcaaaagc gcccggcgaa tgccttcaga
     2341 ttcaatccct tcttgcnnnn nnnnnncccc tcggcgccaa tctgatcggg ccgcagaccc
     2401 gccgtatcag ccaaaatcac cggatatccg cccagatcca gatgcacttc gataacatcg
     2461 cgtgttgtcc cggccatctc ggaaataatc gccacatcgc gctgggccaa cacattaacc
     2521 agcgaagatt tccctacatt cggtgcccca atcaccgcca catgaatccc gtcacgcaac
     2581 cgttctccgc gccggttatc attcaaatgt tcgctcattt cctgcgaaat tgcgctcaaa
     2641 tccgccgtaa tttccggcgc tatttcctgc ggtaaatcct catctggaaa ttcaatctcc
     2701 gcctccagat gcgccaaagc tcgcttcagc cgatccgccc acccctcata aaggttcatc
     2761 agaacgccgc ccatctgcaa gagcgcctgt aagcgctggg cctgcgtctc ggcatcaatc
     2821 aaatcagcca ccgcctcggc ttcagtcaaa tccatcttgc cgttttcaaa cgcccggcgg
     2881 gtaaattcac ccggttcggc catccggtgc ccatcaagac cagccaacgc cgccaaaacc
     2941 gcatccacca cggcccgccc gccatgcaga tgatattcca ccacatcttc acccgtatag
     3001 ctcgccggtg ctttaaacgg caacacaagg gctctatcga gcgtttcacg tgaatcttcg
     3061 ttttttaaat ccaccagcgc cgcctttcgg gggataaaat ccgcccgacc cgaaagcgcc
     3121 tgcaaacccg cccaactctg cggccccgac acccggatca ccgccacgcc ggcccggccc
     3181 ggcccactgg ccaatgcaaa aatcgtctca ctcatttatt ccggctcacc tgtcttcaga
     3241 cccatctggc cccagaacat tttctgcata tcgctcatat tctgaagtgc ggccggaaac
     3301 cacgttttca tcatcgcttc cgggtccatt gcctgaacat tttcctgcat cttggcctgc
     3361 agctcctcca tcatcgcctc ttgcaacggc gccacatccg gcaaacccaa aaatttccgt
     3421 gcttcctccg gtgtgcagtc gatatcaaaa gtcactttca ttgatctcat cccatctttt
     3481 atgctcttat gatttcaagc ttttaacaca gataaagcaa aactacaggg gttaaagatg
     3541 aacgacgagc cgccatatcc acaaaacccg cctgctgatc aacctaaaac cggctctggt
     3601 tccgaagcgc tcaacacccg catctatcat tccgaaattg accctgaact caagaccaaa
     3661 gccctacttc aaatgtgcga agccatcaaa ggcaacggct taaaaaacga agatgtcgat
     3721 atcaccgcct atgacggcga aacccttgaa ttcctggcta tcaccgaaat gaagctcgat
     3781 accaaactct ccatcaagcg ccaggccgga aaaatcactg gcccgacgtt agtggccaat
     3841 gcaggcgcct ttcaggaagc cgtcaataaa gaaacctaca aaatcaccca tagtcttgat
     3901 gtcgttgaac gcatccgcga cgcagtgttg gagcgccctg ataaaggact gtgtcttaaa
     3961 aacatactga taaaacttcc atttttgcat aaagattttg tcacccatga accctgcagt
     4021 gcctgcgctt ccaaaggcaa aatcacctgt gtgcgttgtc atggcaaagg ccgggaaccg
     4081 tgtccacgct gccacgcacg cggctcagaa acctgcccta cttgtggtgg acgccaatac
     4141 atcctcggcc caaacaacca aaatcaacaa tgcatgcgct gcaacggaac cggccggatc
     4201 ggttgcagcc aatgccacga aagccgtgaa gttcaatgcg ctatgtgcaa agccgttggc
     4261 accatgcaat gcagacaatg caacggacac gcctggaact ctgtactctg ccgcgcagaa
     4321 atcaatcccg tcggtcttta cagtttcgat cgcaccacca ttccacaaca agcgcttgaa
     4381 aagcttgatt tactgggatc agacatccag caccattcca acattcaaat cattcaccgc
     4441 cacaaagaac tggaacaaga acgcaacgac atatcgattc cttatcacat caaggtcccg
     4501 atggcccatg tcaccttcat gctaaaagaa aaactggaaa tcccggcttt cctttttggc
     4561 acacaggcaa aactttacga tatgccgcct ttcctagaaa aaattaccgc gcccggcatc
     4621 aaagcattaa aagccgccgc aatggggcag ggcgacgctg caagccttat tcaaaaagcc
     4681 ggacgttacc gcaccttacg cgaaatcttg cttgcaacat ccaaacttgg cccaaaaaaa
     4741 accttgaaag tcatcgaaga aaaaaacccg atcggccttt ctgaagacac cacacgtaaa
     4801 cttgtgttaa tggcctgtaa agccattggg tttttaggca aaaaaccgcg ccagatcagt
     4861 atcgcgcttg ggcttctgat aaccgcagga ttaggcgcct tctattggta tatcggcaaa
     4921 gctgcgcttt tgccctattt accacacaca aacaacattg cactcttaac ggacggtata
     4981 gcaggcttga ccgctctggg cgctggatac gtaacagcga aaacctacct caaacgcgcc
     5041 atgcaaaaat ctttggaatc tttattctct aaataatgag tttcaaaaga agggaggtcg
     5101 tctccggaaa acctgtgtgg gaggcttccg aaagacgacc agcgacgcga gggacagtca
     5161 ctttagtatt tgcggcacat cttcatattt cgttgaaccc tttgcctgta ataaataagg
     5221 caaataggga gaagcaatta tcgagcccaa cgaaattcac tgatagcctt aagcgcctgt
     5281 tctttctggc attggcgata agcggtcacc gcgagagcag aacggtcacc agcgaaagtg
     5341 ccagatgttt gccagacgtc aagacgcggt ctatcagcac gtgacttggc tttttgcggt
     5401 ggcgagaggg caaaacgcac accaagaacc aagccgagag ctgccgcttt tccagccgag
     5461 cgctggttcc gatccgtgac agatacgggg gaagtatgat ggatggattt gagcaaggaa
     5521 aggcatgaat ccgccacggt cggagaagag ggttgagaag gataactggt cgtcaaagcc
     5581 tgttttggat catcaaacgc ggtctgatcc tgaacactat caagcgtttc aaaagaccag
     5641 gccggtccta cagttaaaca aagaagaaga accagagaaa ccaacacggc acctggaata
     5701 taggcagccc gaggatcagt tttccagcaa ttggaatatt tttgagtttt catgttaccc
     5761 acctaagtct tacgcactgt tttatttttt tatgacctga cttaagacgt gaacccattt
     5821 attgttttta tataaccttt ttgataggcc aggcttatac ctttatccca tcacacaatt
     5881 ataattatgg caaatacttt cgtttcgatt ttgtattgtt tttcaagtga ttacatggaa
     5941 aatttgctaa aaaattgata caattacaat tcaaatacaa ttgtatgact ttctttttat
     6001 ccacctgaga tcagttatga tacgtttata gagcattaaa atcacactgt gattcatcat
     6061 gaccgaaata aggaaacact accatgcaca tcgccgacat gcctgagttt aaaaaccgta
     6121 agcaggtcct aacctgcaaa ccagacgata ccgtttacga tgttgtcgtt atgatggcct
     6181 tcaaacattt cggcgccgta gcagtcacag ataatgacaa actggttggt atttttaccg
     6241 agcgtgatct attggtccgc gttgtcgggc agggccgtga tgttgaaagc acaaaaattt
     6301 cagatgtcat gtcaaaaggg attgaaaccg cgaccgccca cgattctgcg cttttatgta
     6361 tgggccgtat gaatcatggc agatttcgcc atatgccggt cacagataat aacggaaaac
     6421 ttaccggcat gctctcccag cgcgacttca tcgcctttac catgcgcgaa ttaaaagaaa
     6481 aattagcaga ttaatataac ctgtgatgca gattactgat tcatcgcatt aaagaattca
     6541 tcgttattct ttgtctgttt cattttgccg agcaggaatt caacagcgtc aacggtgccc
     6601 atcggcgtaa gaatacgccg caatacccac attttctgca aagtatcgcg atcaaccagc
     6661 aattcttctt tccgcgttcc tgatttttga atatcaattg ccggaaacac acgtttatcg
     6721 gaaatcttgc gatctagaac aatctcggag ttgcctgttc ctttaaactc ttcaaaaatc
     6781 acctcatcca tgcgtgaccc ggtatcaatc aaggcagtag cgataattgt caggctgcca
     6841 ccttgttcga tattgcgcgc tgcgccaaag aatcgctttg gccgttgcaa ggcattggca
     6901 tccacacctc ctgtcaaaac cttaccggaa gagggaacca cagtattata agcccgcgcc
     6961 agacgcgtaa tggaatcgag caaaatcacc acgtcgcgct tatgttcagt taggcgcttg
     7021 gctttttcca tcaccatttc cgcgacctgt acatgccggg aagctggctc atcaaaagtt
     7081 gaagacacaa cttcgccgcg cacgctgcgc gccatatccg tcacttcttc cggacgctca
     7141 tcaatcaaca aaacgatcag ataagcgtcc gggtggtttt ccgcaatgga cgttgcaata
     7201 ttctgcaaca tcacggtttt acccgttctt ggcggcgcca caatcaaagc ccgctggcca
     7261 aatccgatcg gcgaggtcaa ctcaatcacc cgctgaacat tgtccttttt ggttggatca
     7321 tcaagctcca gcttgatttt ccgctcagga tacagcggcg taaggttatc aaaatttatc
     7381 cggtggcgca gtttatcagg cgtttcattg ttaatcgaac caaccttaag cagcgcaaaa
     7441 taacgctcgg aatctttcgg cgcacgaatt tcgccctcaa ccgtatcccc tgtgcgcaga
     7501 ccaaaacgac gaatttgcga cgggctgaca taaatatcat ctggccccgg caaataattt
     7561 tcttccgggc tgcgcaagaa accaaaacca tcctgtaaaa cctctaaaac acccgcccca
     7621 aaaatcggcg ttccttgcgt tgcaagctgt ttcaggattg caaacatcat atcctgctta
     7681 cgcatcgcgc tacagttttc aatctcaagc tcttctgcaa aagccaaaag ttcagccggt
     7741 gagcgagttt taagttcttg taaattcatg tgattttcgc tttacaatcg catttgcgct
     7801 gttgttgaat gtgatattaa gatttgaatg tataactaac ttttttgttg gaaaatattc
     7861 cgtttatcgc gccagctcct ctagaagcct tactcagcgc taacttctct ctaattcgtg
     7921 gccgcaaaac acggctcaaa tcaattgcgt ccaaaatgtt atggattgat ccttaaagat
     7981 acttccctat taatcacaag gataaagcgc tgtcaagcac ttatcacatt attttactgt
     8041 aagggcttaa agtttctaaa accttaactt gtgacaatga acacccccgc cccaaggggc
     8101 gaggtaccac gtcattgcga accgccaaag gcggtgaagc aatccagaat ttatccttgt
     8161 ggcactggat cgcttcgctt cgctcgcgat gacgattacg tcgcaagcgg cgaggaatta
     8221 aacctgaaag agattaaaaa ggctgcacca cagcaaagat cacaatccca atcatgagca
     8281 ccgtcggaac ttcattaaga atgcgataat atttactgct cttggtgttt tcgtcatgcg
     8341 caaaacgctt cacatccttg gcaagcatac catgaaccgc cgacatcaac accacaaaca
     8401 acaatttcgc atgcatccag ccttgccctt ccagcatccc cggattggcg taaagcatcg
     8461 aaagaccaaa cagccaactc acaatcatcg ccggattaat aatgactttc aacaaacgcc
     8521 gttccatcac cttaaatgtc tcagacatct cagaacccgc taccaccgct gtgtgataaa
     8581 caaacaaacg cggcaaatag agcatcccag ccatccaggc aatcacactg ataatatgca
     8641 gtgcgcgcac ccattcataa tgttgaagta aaaaatcctg cataatttta agccgcctcc
     8701 ttacatatcg aacaaaccat gaccgctctc gtttttacgc aacattttcc aaattcttcc
     8761 gaacagggac atttaaaccc ttcaggagca acgccatcct tatccaaata ctcctccaca
     8821 agcccggcaa gcccttcaat gaaatccgga tgcgtgccta ccgtctgcgc ccggtaatat
     8881 ccggaaacac caagcttttg agcttcttcc ttatattcca catccagctc aaccagcgtt
     8941 tcaacatgct cctgcgtaaa agcatgcgga tacacaatca cagccctttt atcctgcgca
     9001 gctttcacca aagcctcacc caacgacggc ccgatccatt tttgcggtcc cacacgcgat
     9061 tgataacaat attgataatc cagaccttca atgcccagct ttttcacaat cgccttagcg
     9121 ctttgctcac attgccattg ataaggatca ccatccttaa taattttctc cggcaagcca
     9181 tgcgcagaaa acaaaacccg tggagcctca tgcccgtctt taagtgcctg atcatagcac
     9241 gccttgatat tttccgccga cgcactcaca aacccggcat tttcgtgata acaacacacc
     9301 atagcactcg gaatttcaat acccgcctca aacaacgctt tattccactg ctccagcgac
     9361 gaccacgttg ttgtcgttga aaattgcgga taaagcggca acaagataat tttatcagca
     9421 ccccaatcac gcacctcacg caccacttgc gcagccatcg gatgccaata acgcatacat
     9481 gtaaacacct taaattttgt ttcacgtgaa tctgccttca gcactttttc caatgccttg
     9541 gcctgcgcat tcgtattgtc caacaagggc gatttatctc caagctcacc atagctttca
     9601 ccagcttcgc gcttggaacg ccgattcgca atcatcgtcg ccaacaaaca acgaaacggc
     9661 acaggcaaac tgataatatt tttatccatg aaaaaattta acagaaacgg acgaatcgcg
     9721 tttttagaat ccggtccacc gagattaaac aacacaaccg cgattttctc agccatattc
     9781 taaaattctc ccctgatcaa cttcaccaac tgct
//
LOCUS       DAOSWR010000077         9808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4389_length_9808_cov_5.611402, whole genome shotgun sequence.
ACCESSION   DAOSWR010000077 DAOSWR010000000
VERSION     DAOSWR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_9808_cov_5.611402"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK513_06705"
     CDS             complement(<1..397)
                     /locus_tag="PK513_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOO82174.1"
                     /translation="MAKLENIIPKRRSELLMPAGSLEKLKIAVLYGADAVYLGTPDMS
                     LRTKSAFTLDEVVEGIKFAHAHGKRVYLTLNLFTHNKDIAKLPEYLDTVKKVKPDGLI
                     IADPGVFQFVKEQAPELELHISTQANICSW"
     gene            complement(440..1621)
                     /locus_tag="PK513_06710"
     CDS             complement(440..1621)
                     /locus_tag="PK513_06710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO82175.1"
                     /translation="MKTQYDVIIVGAGAAGLMCAIEAGKRGRKVWLIDHAAKLAEKIR
                     ISGGGRCNFTNIHTKPENYISTNPHFCKSALKQFTPQDFIALVEKHGIGYHEKKLGQL
                     FCDDSAQQIIDMLLSECSKADVKINNETTVETIEYAKDHYYLSTSRGTAACQSLVIAT
                     GGLSIPKIGASKFGYDVARRFGINVIETAPALVPLTFQTTLLERCRALSGLSVKASVT
                     CNQTTFCEGLLFTHRGLSGPSILQISSYWREGDEIAINLAPETDVFAFLKKRKTSQGK
                     QDIQTALSHILPQRLAQSICEAENINGRLADLPDKTLRKLAATTNGWHIKPAGTEGYR
                     TAEVTRQGVDTSALSSKTMEAKAQPGLYFIGEVVDVTGHLGGFNFQWAWSSGYAAGQH
                     V"
     gene            1645..1887
                     /locus_tag="PK513_06715"
     CDS             1645..1887
                     /locus_tag="PK513_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007141327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO82176.1"
                     /translation="MDWVVYILKCGDGSLYTGITNDFPARVAAHEAGTAAKYTKGRGP
                     FALVYQELCEDRSAASKREMQVKKLSRAEKLQLCGL"
     gene            complement(1884..2111)
                     /locus_tag="PK513_06720"
     CDS             complement(1884..2111)
                     /locus_tag="PK513_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOO82177.1"
                     /translation="MNNTALNKVEETLAHQEQQINDLSDMVITQGRDIEALKKQIRKL
                     QDKISSSAEDNNNEKPLSATEYAARNKPPHY"
     gene            complement(2089..3963)
                     /locus_tag="PK513_06725"
     CDS             complement(2089..3963)
                     /locus_tag="PK513_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO82178.1"
                     /translation="MPPPLLLSVKDALVRYSETPVFENLSFNIHQGSRIALVGKNGAG
                     KTTLMNIITGRQDLDDGERWEDVGVSIGYLHQDIIPQDGQNVFDFIFTDMKAEEKELH
                     TYKVDIITEALHLDPQSQMTRLSGGQLRRAGLARALVEEPDILLLDEPTNHLDLEAIE
                     WLETYLKAYRGTLMCISHDRTFLANITNQVFWLDRGQLKISPRGFKYFDEWSTALFEQ
                     EERELKNRKQSVGMEVEWASRGVKARRKRNIRRLEQMREMRDQLKADEAAYRRATAKI
                     NLKPLKDTETNSKIVAEFYNVCKSFEEDSKHIKILDKFSLRIQRGDRIGILGKNGSGK
                     TTFLKLLLGELQPDQGRVKTRKELEFSYFDQKRSDLDPTDTLKKTLSPSGGDYIDVMG
                     KQRHVCGYLKDFMFDPSRAQDKVSQLSGGQKNRLMLAKILASPKTCLILDEPTNDLDM
                     DTLDMLEEILSQYKGTLIVVSHDRDFLDQTVTKILTFKGDGKIERCIGGYSDYLAQHN
                     ASVFPKPEKKNAAQKPPQEKPVEAEKPKLPVKRQRLTYKLERELKQLPAKIEKAEKEI
                     ADLSERLADADFYKRDPDGFHNATKALSEEQARLERYETRWLELEEMKESPHEQHRPQ
                     "
     gene            complement(4001..7249)
                     /locus_tag="PK513_06730"
     CDS             complement(4001..7249)
                     /locus_tag="PK513_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82179.1"
                     /translation="MIRKQTIEIYYKAVKGLLALILSVACLYISVPPDAHAKEETIPN
                     EFIFEVMLPPTWRISEAVFAYERGGKYYLPINELAAGFNFFIDTEQDRGLATGFAGRE
                     ENNFTIDHGRKEIIIKNQRSVLNDDAILISEYLATDDLYVQLEVLNQIWPVDMSIDLS
                     ALKIIVETEEKLSFMRDKEREEKLEKVVSRKATQEALKKKLPKREHPYQFFGSPVMDF
                     QALYTYDDKEDTLTGSNIFSGVQQLGKMIAQFSANIGMNEDKKLERPESIRFKLSRKS
                     ADKDYLVPGIRSIETADVSLRQRNLISNTENGRGLIISNDNRDRFNEFSQITVEGIGP
                     PGWEIELYNNEELLEFSTVPDNGQFFFEDVTLNFGNNQIRILLFGPQGQVREDIRSYT
                     AGGNMLSPGSFIYKAGFLDADREFILLDNAPRTEPRGVVKNLETSYGVNEWLTVFGNY
                     AQLPEQDKDHTYMTAGASISTPIGLVDAEAYNEIGGGNALALDYITKFLGVRSNFGAA
                     FFNDFESQDAGFGNNHKTFEAKAQLNKTFKIATLPLGLRLNTTHTERKSSLPITTIDT
                     AQTLSRAGLRLSHNTSTRLNDYSHERSTGSFTTTWREKDWQLRGTFGYDIYPKFDFGS
                     FNSELRYRPDEKYQGSLTLGHNFQTSDYNAGLQVGYDFDTILGTLESRYQRGEGWDFI
                     LRATTSLNPYTPDGRYGFSSDQKREYSPVRAQVFLDKDLDGVFGEGDEPLPDARLRIG
                     TTNSQITADENGMIIANAPADRLTNFQIDPSSLEDPYFIPATNGFSAVPAKGSVIEVS
                     FPIVETGAIEGTVLRGDNDRIVAGLTLELFDENNEKVGKSVSAFDGYYAFEFIKPGRY
                     KIQADPSHKGVRLLDNETTINPDDLFVYGNDLYAQILTLGQNEKPEQKLPYGPFAQDI
                     APAAGNSAEIFGPFKPEQSPEQKEETKAAKVVQPINEETKKSETQTETTAKLTPPADP
                     VKFTTTVQQARFKAHDGHARLILETNEDIQYEILQSDSPQDIQILINDTLWQPPTSKN
                     ISHGGHTYIMSAQQTEAGTLLSISSQQPLSLKRDLVLQNQTSQGARLLLDIN"
     gene            complement(7384..8265)
                     /locus_tag="PK513_06735"
     CDS             complement(7384..8265)
                     /locus_tag="PK513_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82180.1"
                     /translation="MSLKTKKTPPRNATMLQKSVWIVGICLILGFSVFMLTSQPAYAV
                     RVSLKRVVFDESKRSEILTIINTTAEPQTYRLGWRRYRMSGGKGLSVIKEGEDDSDIL
                     WADDMIRFAPRRVTIPPGGSQQIRLLLRRPANIQQAEYRSHLWIVSESKPAKFDAEPG
                     NKKAMRLAVQPAISLPVFVRHGDLKVETSISNAQLKRSDKGLNVAFTLDRTGNRSIYG
                     DFDFTCTNQGEELILHQVRGIAVYTETNMRNLDFDIPLSENTAANCSSIKITYRADPN
                     DADFAGKTLAEATATLQ"
     gene            complement(8339..8905)
                     /locus_tag="PK513_06740"
     CDS             complement(8339..8905)
                     /locus_tag="PK513_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOO82181.1"
                     /translation="MRKDSQKPVPNSLKKLATGSLVTLSMFSALALRSVIASTATLPI
                     IVRLVRAIEVTVATSLDFGTLAMDPEAAGRAAINPSLNRLILDDGNSLSLAGGTPKVG
                     RLRIKGSAFPVSISIEDTAVHLTNGLTNVTVNNFNFVSADGGTRVTITPQAGQVSFTV
                     PVGATLHTKPGQSEGTYVGTTRIFANFQ"
     gene            complement(9074..9604)
                     /locus_tag="PK513_06745"
     CDS             complement(9074..9604)
                     /locus_tag="PK513_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOO82182.1"
                     /translation="MKNSIRKVALSSATVIAAGALGSAANAATASVAIQAIVLDPVQI
                     TANNSLNFGSVTESGAGGTIIVDNGGFNSVTGGVTSIGGTIQPGTFTLKGSTGRNIQV
                     DVANVATISSGVNTMTVNALVLYNPAGTITGDPITVTLGAATVAGFELGGTLNVGAGQ
                     AAGTYTGNVAVTAAYN"
BASE COUNT         2109 a         2074 c         2673 g         2952 t
ORIGIN      
        1 gccaggaaca gatattggcc tgagttgaga tgtggagttc aagctcgggg gcttgttcct
       61 tgacgaattg aaacacgccg ggatcggcga tgatcaggcc gtctggcttc acctttttta
      121 cggtgtccag atattcgggc agtttggcaa tgtctttgtt atgggtgaac aggttgaggg
      181 tcaaataaac gcgcttgccg tgggcatggg cgaatttgat gccttcaacg acctcatcga
      241 gggtaaaggc cgatttggtg cgcagggaca tatccggcgt gcccaaatat accgcatcgg
      301 cgccgtaaag caccgcgatt ttgagctttt ccagcgatcc ggcgggcatg agcagctcgg
      361 agcgccgttt cggaataatg ttttcgagtt tggccataac ccgcgcacat aatcatattc
      421 agctcttcgc gtaaagtttt tatacgtgtt ggccggccgc ataacctgag gaccatgccc
      481 attggaaatt gaagccgccc aaatgtccag taacatctac gacttcgccg atgaaataca
      541 ggcctggctg ggctttggcc tccatggttt tggaggataa tgcacttgta tcaacgcctt
      601 gtcgggtgac ttcggcggtg cggtatccct cggtgccggc gggtttaatg tgccagccat
      661 tggtggtggc tgcgagtttt cttagcgttt tatcaggcag gtctgcgagg cggccattta
      721 tgttttcagc ctcgcagatg ctttgcgcga ggcgctgggg tagaatatgg gagagtgcgg
      781 tttgaatgtc ttgttttcct tgcgatgttt tgcgtttttt taagaatgcg aagacatctg
      841 tttcgggagc gagattgata gcgatttcat cgccttcacg ccagtaggat gagatttgca
      901 agattgatgg gccgctgagg ccgcgatggg tgaagagcag gccctcgcaa aaagtggttt
      961 gattgcaggt gacgctggct ttgactgaca ggccgcttaa ggcgcggcag cgctccagca
     1021 atgtggtttg aaatgtaagg ggtacgagtg cgggggcggt ttctatgaca ttgatgccga
     1081 agcgccgagc gacatcgtag ccgaatttgc tggcaccgat tttgggaatt gacaggccgc
     1141 ccgtcgcaat gacaagggat tggcaggcag cggttcctcg tgaagtgctc aggtaatagt
     1201 ggtctttagc gtattcaatg gtttcaacgg ttgtttcatt attgatttta acgtctgctt
     1261 tgctgcattc actcagcagc atgtcgataa tttgttgcgc agaatcgtcg cagaaaagct
     1321 ggccgagttt cttttcgtga tagccaatac cgtgtttttc aaccaaagca atgaagtcct
     1381 ggggtgtgaa ttgcttgagt gcggatttgc aaaaatgcgg gtttgtggag atgtagtttt
     1441 cgggcttcgt atggatgttt gtgaaattac agcgcccgcc gccggagatg cggatttttt
     1501 ccgccaactt tgcggcatga tcgatcagcc agactttgcg gccgcgcttg cctgcttcga
     1561 tggcgcacat taacccggct gcgcctgctc caacaatgat tacatcatat tgggttttca
     1621 tgttttgcgt tgtatcataa tttcatggat tgggtggttt atattttaaa gtgtggtgac
     1681 gggagcctgt atacgggtat taccaatgat tttcctgcac gtgtggcagc gcatgaggcg
     1741 ggaacggctg cgaaatatac gaaagggcgc gggccgtttg cgcttgtgta tcaagagctt
     1801 tgcgaggatc gtagcgcggc gtccaagcgt gagatgcagg ttaaaaaatt gagtcgggcg
     1861 gaaaagctgc agctttgtgg gctttaatag tgtgggggtt tattgcgtgc ggcgtactcg
     1921 gttgccgaga gcggtttttc gttgttattg tcttctgcgg agctgctgat tttgtcttgc
     1981 agtttgcgga tttgtttttt aagggcttcg atatcacgac cttgcgtaat gaccatatcg
     2041 cttagatcat taatctgctg ttcctgatgg gcgagggttt cttcaacttt attgagggcg
     2101 gtgttgttca tgaggtgatt ctttcatttc ttcaagttca agccatcggg tttcatagcg
     2161 ttcgagccgt gcttgttctt cggagagcgc ctttgttgca ttgtggaaac cgtctggatc
     2221 gcgtttgtaa aaatctgcat cggcgagtct ttcggataga tcggcaattt ctttttctgc
     2281 tttttcgatt ttagccggga gttgtttaag ttcgcgctca agtttatagg tcaggcgctg
     2341 gcgtttgact ggcagctttg gtttttcagc ttctaccggt ttttcttgcg gtggtttttg
     2401 tgcagcattt ttcttttcag gtttgggaaa aacgcttgca ttatgctgtg cgaggtaatc
     2461 tgagtatccg ccgatgcagc gctcaatttt gccatctcct ttgaaggtga gaattttggt
     2521 gacggtttgg tcgagaaaat cgcggtcgtg ggagacgaca atcaacgtgc ctttatattg
     2581 tgagaggatt tcttcgagca tgtcgagcgt atccatatcg agatcgttgg ttggttcatc
     2641 gagaattaga caggttttgg ggctggccag gattttggcc agcatcaggc ggtttttctg
     2701 gccgccggaa agctgggaga ctttatcttg tgcacgtgat gggtcgaaca tgaaatcctt
     2761 caaatagccg cagacatggc gttgcttacc catgacgtcg atgtaatcgc cgccggaggg
     2821 agagagagtt tttttgagtg tatcggtggg atccaggtcg ctgcgttttt ggtcaaaata
     2881 ggaaaattca agctctttgc gggttttgac gcggccttgg tcgggctgta gctcaccgag
     2941 caggagtttg aggaaggtgg ttttgccgga gccatttttg ccgagaatgc caatacggtc
     3001 gccgcgctgg atgcgcaggg agaatttatc aagaattttg atgtgtttgc tgtcttcctc
     3061 aaaggatttg cagacattat agaattccgc gacgattttt gaattggttt cggtgtcctt
     3121 gagtggtttg aggtttattt tagcagtggc gcggcgatag gcggcttcat cggctttgag
     3181 ctggtcgcgc atttcgcgca tttgttccag cctgcggata ttgcgtttgc gccgggcttt
     3241 gacgccgcgg ctggcccatt cgacctccat gccgacagat tgtttgcggt ttttcagttc
     3301 gcgttcttcc tgttcaaata gcgcggtgga ccattcgtcg aaatatttaa aaccgcgcgg
     3361 gctgattttg agctgaccgc ggtcaagcca gaatacctgg ttggtgatgt tggcgagaaa
     3421 ggtgcggtcg tgactgatgc acattaatgt gccgcgatag gctttgagat aggtttcaag
     3481 ccattcaatg gcttcgaggt cgagatggtt ggtcggctca tccagcagca ggatgtcggg
     3541 ttcttcgacc agcgcgcggg cgagtccagc acggcggagc tggccgccgg aaagccttgt
     3601 catctggctt tgcgggtcga gatgcagggc ttcggtaata atatcgacct tataggtgtg
     3661 aagttctttt tcttcagctt tcatgtcggt gaagataaaa tcgaagacgt tttggccgtc
     3721 ctgagggata atatcctggt gcagatagcc gatggagacg ccgacgtctt cccagcgctc
     3781 gccatcgtcg aggtcttgcc tgccagtgat gatgttcatc agcgtggttt tgcctgcgcc
     3841 gttcttgccg accagcgcga tgcgggagcc ctgatggata ttgaaagaca ggttttcgaa
     3901 tacgggggtt tcgctataac ggacaagcgc gtctttaacg ctgagaagaa gtggtggcgg
     3961 catggggatt tatttaacgg taaaaacggt agtgagcaac ttaatttatg tccagtagca
     4021 gccgtgcgcc ctggctggtt tgattttgta agacgagatc gcgttttagg ctgagcggct
     4081 gttggctgga aatgctgagc agcgttccgg cttcggtttg ctgcgcactc atgatgtatg
     4141 tatggccacc atggctgatg tttttgcttg tgggcggttg ccagagcgtg tcgttgatga
     4201 ggatttgaat atcttgtggg ctgtcgcttt gcaaaatttc atattgtata tcttcgttcg
     4261 tttctaaaat taagcgtgcg tggccgtcat gagccttaaa gcgggcttgc tgaacggttg
     4321 ttgtaaattt taccggatcc gccggaggtg ttagcttggc agttgtttcg gtttgcgttt
     4381 cagatttttt tgtttcttcg ttaattggtt gaacgacttt tgcggctttt gtttcttctt
     4441 tttgttcagg gctttgttca ggcttaaagg ggccgaatat ttcagcagag tttcctgccg
     4501 caggtgcgat atcttgtgcg aaaggtccgt agggcagctt ttgttcaggt ttttcatttt
     4561 gtccaagtgt aagaatttgt gcgtataggt cattaccata gacaaacagg tcgtcagggt
     4621 taatcgttgt ttcattgtcg aggagccgga cgcctttatg ggaggggtct gcttgaattt
     4681 tgtagcggcc cggtttgatg aattcgaagg cgtagtaacc gtcaaaggcg ctgacgctct
     4741 taccaacttt ttcattgttt tcgtcgaaga gttctagcgt tagccctgca acaattctgt
     4801 cattgtcacc acgcagtacc gttccttcaa ttgcgccggt ttctacgatt gggaaagaga
     4861 cttcgatgac actgccttta gcaggcacgg cgctgaaacc gtttgtggcc ggtataaaat
     4921 aaggatcttc cagcgatgat ggatcaattt gaaaattggt cagacgatcg gccggggcgt
     4981 tggctataat catgccattt tcatcggctg tgatttgtga atttgtggtg ccgattcgca
     5041 ggcgcgcatc cggcagaggc tcatcgcctt ctccaaatac gccatctaga tctttatcga
     5101 ggaagacttg cgcacgtact ggagagtatt cgcgtttttg atcgctgcta aagccatagc
     5161 ggccatcggg ggtgtaaggg ttgagggatg ttgtcgcacg aaggataaaa tcccatcctt
     5221 ccccgcgttg gtagcggctt tcgagtgttc caagtatcgt atcgaaatca tagcccacct
     5281 gtagtccggc gttgtagtcg gatgtttgga aattatggcc aagcgtcaag gagccttgat
     5341 atttttcatc cgggcgatag cgtaattctg aattaaagct gccgaaatcg aatttgggat
     5401 aaatatcgta accaaaggta ccgcgcaatt gccagtcctt ttcgcgccat gttgttgtga
     5461 agcttccggt ggagcgttcg tggctataat catttaatcg tgttgatgta ttgtggctga
     5521 gccttaaacc tgctctggac aatgtttgtg cggtatcaat tgttgtgatt ggcaggctgg
     5581 atttacgttc tgtatgtgta gtatttaggc gcagccctaa cgggagggtt gctattttaa
     5641 atgttttatt gagttgcgct ttggcttcaa aggttttgtg attattgccg aaccctgcat
     5701 cctggctttc aaagtcattg aagaatgctg caccaaagtt gctccgcaca ccaaggaatt
     5761 tagtgatgta gtccagagcg agggcgttac ctccgccgat ttcattatag gcttcggcgt
     5821 caactaatcc tatcggcgta cttattgacg ctcctgctgt catataggta tggtctttgt
     5881 cttgctcggg gagctgtgca tagttgccga aaacggtaag ccattcgttg acgccgtaag
     5941 atgtttcaag gtttttgacg acaccgcgtg gttcggtacg cggggcgtta tccagcagga
     6001 taaattctcg gtccgcatcc agaaagcctg ctttataaat gaaggatccc ggcgaaagca
     6061 tgttgccgcc agccgtgtag ctacgtatgt cttcgcgcac ttgtccctgt ggtccgaaca
     6121 gcaggattct gatctggttg ttgccaaaat tcagggtgac atcctcgaag aaaaactggc
     6181 cgttatccgg gactgtgctg aactcgagca gctcttcgtt gttataaagc tctatctccc
     6241 agcccggtgg cccaatgcct tctactgtga tttgactgaa ttcattgaag cggtcccggt
     6301 tatcattgga aataataagg ccgcgtccgt tttcagtatt ggatataagg ttacgttggc
     6361 gcaaacttac atcggcggtt tcgatactac gaatgccggg gacgaggtag tctttgtctg
     6421 cggatttgcg gctgagcttg aagcgaatac tctctgggcg ttcgagtttt ttatcttcgt
     6481 tcatgccaat attagcagag aactgcgcga tcattttgcc aagctgttgg acgcctgaga
     6541 aaatatttga ccccgtcagg gtgtcttctt tgtcgtcata tgtgtaaagc gcctgaaagt
     6601 ccatgactgg ggacccgaag aattggtagg ggtgctcgcg ttttggcaac ttcttcttaa
     6661 gtgcttcctg tgttgcttta cgagagacga ctttttccag tttttcttcg cgctctttat
     6721 cacgcatgaa ggatagtttt tcttctgttt cgacgatgat cttgagggcc gataggtcga
     6781 tgctcatgtc gacgggccag atctggttta atacttcgag ttgaacgtaa agatcatcgg
     6841 ttgccaggta ttccgagatt aatattgcat cgtcgtttag gacggatctt tggtttttaa
     6901 tgattatctc tttgcgaccg tggtcaatgg tgaagttatt ttcttcgcgg ccggcaaagc
     6961 ctgtggcgag tcctcgatct tgttcggtgt cgataaagaa attgaatccg gcagccagtt
     7021 cgttaatggg taaatagtac ttaccaccgc gttcataggc aaaaactgcc tctgaaatgc
     7081 gccatgtcgg agggagcatg acttcaaaaa taaattcatt gggaatggtt tcttctttgg
     7141 catgtgcatc ggggggcacg cttatataca ggcatgcaac gcttaagata agcgccagta
     7201 ggccttttac ggctttgtaa tagatttcaa tggtctgttt ccgtatcact gaaacgtccc
     7261 tcgtccatct tttgcgtttt gcataaagca gtttccgaaa gtgaaaacat gctacccgta
     7321 aaagatatta gcaagcacta tgccaatgtt gtggaaaagg ggatttttag tataaaattc
     7381 agtctattgt agggttgctg ttgcctctgc gagggttttt cccgcaaagt cagcatcgtt
     7441 tggatcagca cggtaagtga tttttatgct gctacagttt gctgctgtat tttctgataa
     7501 cggaatatca aaatccaggt tgcgcatatt tgtttctgtg taaacggcga taccgcgcac
     7561 ctgatgcaaa ataagctctt caccctgatt tgtacaagtg aagtcaaaat caccgtatat
     7621 actgcggttt cctgttctgt cgagcgtaaa agctacgttc aagcccttat cactgcgttt
     7681 tagctgggcg ttggaaatgc tggtttcaac ctttaaatcg ccgtgacgca caaaaacagg
     7741 caatgaaatg gccggttgta cggctaggcg cattgctttt ttattgcctg gttctgcgtc
     7801 gaatttggcc ggcttgcttt cagaaacaat ccaaagatgg gagcggtatt ctgcttgctg
     7861 tatattggct ggacggcgca ggagcaggcg aatttgttgg gaacctcccg gaggaattgt
     7921 gactcggcgc ggagcaaaac gaatcatgtc atcggcccaa agaatatcgc tgtcgtcttc
     7981 gccttctttt ataacgctca gcccttttcc tccgctcatt ctatatcgcc gccaacctaa
     8041 acggtaggtt tgcggttcgg cggttgtgtt gatgatggtt agaatttcag agcgtttgct
     8101 ttcgtcaaaa accacccgtt ttaagctgac gcgaacggcg taagctggct ggctggtcag
     8161 catgaaaaca ctaaagccta atatgaggca tatccctacg atccatacgc ttttttgtag
     8221 cattgtggcg tttctcggcg gtgttttttt tgttttcagg ctcacttgta accttccctt
     8281 ttttaaccgt gcaaaatttg catcatttta cttttatatt caatatttta aagaaactct
     8341 actgaaaatt tgcaaaaatc cttgttgttc caacataagt gccttcagat tgacctggtt
     8401 ttgtgtgaag tgttgcgcca acaggcacag tgaaagagac ttgtccggct tggggggtaa
     8461 ttgtaacgcg tgtgccgcca tcggcagaaa cgaagttaaa attatttact gtgacgttgg
     8521 tcaagccgtt ggttaaatgt acggctgtgt cttctatgct tatggatacg ggaaaggcgg
     8581 agccttttat ccgcaagcgt ccgactttcg gggtgccgcc ggcaaggctt aaagaatttc
     8641 cgtcatcaag aattaaacgg tttaaggacg ggtttattgc ggcccggcct gctgcttcag
     8701 ggtccatggc cagtgtgcca aaatctaggg atgttgcgac cgtgacttca atggctctta
     8761 caaggcgtac gataatcggt aaagttgcag tgctggcaat tacagagcgc agggctagag
     8821 cactgaacat gctcagcgta acaaggctgc cagtagccag cttctttagg ctatttggca
     8881 cgggtttctg actatctttg cgcatgcata tcctatgctt tttcctgcgt ttatatgatg
     8941 ccgagcttaa caatcgcgaa tcattctaac atggcttggc gattaaatta aaaataaagc
     9001 cgaccataat tgcactttgg tcggctttat ttaatttgta atgaaaacgt aatgttttat
     9061 aagctgacct tgcctagtta taagctgccg ttacggcaac gttgcctgtg tatgtaccgg
     9121 ctgcttgacc tgcgcctacg tttagtgttc cgccaagttc aaagccagca actgttgcag
     9181 cacccaatgt tactgtaatg gggtcgccgg taatggtgcc tgccgggttg tataagacga
     9241 gagcattgac cgtcattgtg ttcacaccag aagaaatcgt tgctacgttt gcgacgtcaa
     9301 cctgaatgtt acgacctgtg gatcctttaa gtgtaaatgt tccaggctgg attgtaccgc
     9361 caatcgatgt tacgccgccg gttacagagt taaaaccgcc attatcgact atgatcgtac
     9421 cgccagcacc ggattctgtt acggaaccga agttcaaaga gttgttggcg gtgatttgca
     9481 ccgggtctaa aacgatcgcc tgaatggcga cgcttgctgt ggccgcgttt gctgcgctgc
     9541 ctagtgcacc tgcggcgatt actgttgccg aactgagtgc gacctttcta atggaattct
     9601 tcattagatt tctccttttt tgactactta cgacttaaat ttttaagtcc ttattagagt
     9661 taatacccaa actgataaca caaagagtga ttgttaagaa cactatgcct cgagtgaggt
     9721 tgtgcagacc taaattcaca ccattgtacc ttctgtttag attaatgcaa gttgtttaag
     9781 ataagtttaa tatagacgta caacggta
//
LOCUS       DAOSWR010000078         9594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4514_length_9594_cov_5.661076, whole genome shotgun sequence.
ACCESSION   DAOSWR010000078 DAOSWR010000000
VERSION     DAOSWR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_9594_cov_5.661076"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK513_06750"
     CDS             complement(<1..222)
                     /locus_tag="PK513_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82183.1"
                     /translation="MFSKFFGKTPKSYEQQRAALASANVKERLSLASNTKTNKEILYY
                     LAEKDPSPKVRKAVAENEAMPVQVSSILAG"
     gene            338..703
                     /locus_tag="PK513_06755"
     CDS             338..703
                     /locus_tag="PK513_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82184.1"
                     /translation="MSAYTNKAGSRVRGAFNAKSIKHNTPLSVYISPKINKSTSDDTT
                     ERRKQYQVSFAAAITRIRLRLNELGIRENTTANTPSHIAVFVNKSQLQKLADHPDIQK
                     FKMNPTDEGHVEGRTINLI"
     gene            complement(712..1848)
                     /gene="zapE"
                     /locus_tag="PK513_06760"
     CDS             complement(712..1848)
                     /gene="zapE"
                     /locus_tag="PK513_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOO82185.1"
                     /translation="MPQRKAPQKLYQSRLDEGILKPDPAQQRAVKSLERLYWELVEIS
                     EHSAACRGWRAIKEKIPFLNTAPGPARGLYMYGGVGRGKSMLMDLFYDCLPNSIPKRR
                     VHFHAFMLELHDYHHARRNSADVCEGMDGILPLFAERIVERARVLCFDEFHVTDVADA
                     MILGRLFRHLFERGVVVVATSNWEPDRLYEGGLQRDRFLPFIALLKEKMEVLYLDSST
                     DYRTKFLAQEGTYFWPLGHETMRHANTVFNGLTDNALPDTDMLEVKGRTIRVDAVARG
                     VARFSFAQLCEQPHGAEDYLEITNNYHTVFVEGVPTLGYDRRNEAKRLMILIDTLYEA
                     GTKLVMTADALPDELYRGHDYAFEFKRTVSRLQEMQSAEYLRRG"
     gene            complement(1802..2575)
                     /gene="tatC"
                     /locus_tag="PK513_06765"
     CDS             complement(1802..2575)
                     /gene="tatC"
                     /locus_tag="PK513_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOO82186.1"
                     /translation="MTENTAQPLTHHLIELRQRLMWVFGAMILGTGICFGFVEQIYSF
                     LVAPLAGAMGEGDSQRLIYTGLTEAFFTYLKVAFFAGIFLTFPILLVQIWGFIAPGLY
                     DNERKAFLPFMVATPILFFAGGAVVYYIVLPMAWPFFLSFQTSAEETALPIQLEARVS
                     EYLDLIMTLIFAFGLCFQLPVALTLMGKAGLVSAEWLASKRKYALIITFIVAAFITPP
                     DVISQILLAVPILGLYELSIFLIRHAAKKSSAKTLSEPS"
     gene            complement(2679..2969)
                     /locus_tag="PK513_06770"
     CDS             complement(2679..2969)
                     /locus_tag="PK513_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOO82187.1"
                     /translation="MLDFGWAELFLVMAVVVFALGPKEIPGLMRGMGRLFRRFQYMKY
                     SISRQFDEFMDAEGLNEAVNFEVRHDGSSDFDEAAEDEDVMKPLKQNQEDLK"
     gene            complement(2971..3714)
                     /locus_tag="PK513_06775"
     CDS             complement(2971..3714)
                     /locus_tag="PK513_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO82188.1"
                     /translation="MEPGLYREVDEIAPRWQFNALALGREVPGLRSYVENRYCIKVAE
                     REGTPVGAVFAWPETKKFPGSRARVTEAYLDNIVVFPDKYREEGIGTALLDAAKGWAE
                     NAGHESLYTKIVNKNKVSLAFYKAVGFSPRSINFGGYNNKVFDSHHVMGFEGSTVFEP
                     NEAIAVVSKKRSSLSWSNFEREAHVLFRPGSGAKMNPSLLKQAQAWATDKGIEHMLVE
                     IPVNDSKSVALFEEAGFTADSTNMEIRLG"
     gene            complement(3877..4059)
                     /gene="tatA"
                     /locus_tag="PK513_06780"
     CDS             complement(3877..4059)
                     /gene="tatA"
                     /locus_tag="PK513_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOO82189.1"
                     /translation="MAPGPLQILIVVLLILVVFGAARLPMIAENLAKGIKSFKQGLKD
                     DDDVPKSIDDQSKKDD"
     gene            complement(4064..4762)
                     /gene="scpB"
                     /locus_tag="PK513_06785"
     CDS             complement(4064..4762)
                     /gene="scpB"
                     /locus_tag="PK513_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO82190.1"
                     /translation="MIAMDELTRIVEALLFASAEPLATADLHEYTPDGADVGAALMSL
                     KRDYEGRGVNLVEIDGKWAFRTSADLAEALAIEKEVSKKLSRAALETLAIIAYHQPVT
                     RAEVENIRGVATHRGTLDALIEAGWVKPGRRRETPGRPLTWVTTNAFLDQFSLESVMD
                     LPGLDDLKASGLLDRRPAIEAIPGSDDLFAGQEALSRVEQDADEPGEIEREALEKAEA
                     DYEQERAEEELEEI"
     gene            complement(4759..4887)
                     /locus_tag="PK513_06790"
     CDS             complement(4759..4887)
                     /locus_tag="PK513_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82191.1"
                     /translation="MAQITNELLYEVLKKLQSDIAELKPMRHEMRDGFATMKSYSE"
     gene            complement(5018..5359)
                     /locus_tag="PK513_06795"
     CDS             complement(5018..5359)
                     /locus_tag="PK513_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82192.1"
                     /translation="MAASFEGGGRMNKATSIIITLVIPIIVSLINLFFFYICVIYFID
                     GIYSDVLYTPNYIFYLRWIWLIVVSLIVLFLSIKGVLLVAKQENEMSMSRGYKFIFAP
                     FCGVVVLCLSL"
     gene            complement(5377..6204)
                     /locus_tag="PK513_06800"
     CDS             complement(5377..6204)
                     /locus_tag="PK513_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOO82193.1"
                     /translation="MNDIAIQNSEFQEDPPRAEKVSEADALLLNIDGYEGPIDVLLEL
                     ARNQKVDLLQVSILQLVRQYLSFMERAKELNLDLAAEYLVMAAWLAYLKSRLLLPREN
                     NEVDPSAEEMAEALQFQLRRLEAMQNAADRLIGQPQLGQQVFARGAPEGVRVHTTTRW
                     DVKLYDLLKAYGDIKKRIEGQSYDLPTFNLMSMENAMSRLSRMLGQLPRKGMHSVWST
                     LESFMPEGVRSKLFGRSALASTFTAGLELVKQGKLEIKQDGLFRPIYMRGVIAGDKA"
     gene            complement(6201..7175)
                     /gene="nagZ"
                     /locus_tag="PK513_06805"
     CDS             complement(6201..7175)
                     /gene="nagZ"
                     /locus_tag="PK513_06805"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOO82194.1"
                     /translation="MIKAVILSCAGAVLSGDERAFFKEAHPVGFILFVRNCESPGQVK
                     ALCDELRACVGWHCPILIDQEGGRVQRLKPPMWRQYPPMKSFGDKAREDMDGALEDLR
                     FTILQLAEELVEVGVNVNCAPVLDVLQDDTHEAIGDRAFSNDGQVVGRLGLSVCRHLL
                     AAGIMPVIKHIPGHGRGKVDSHKDLPHVAESLETLQKNDFDPFRIVVESDVGCRVWAM
                     VAHIVYDQIDNERPASVSSDVIEKIIRQDIGFDGFLVSDDLDMEALGAYGPIAARAAL
                     TIEAGCDAALYCAGDLAVMEKIVKTVPNLSRKARKRLQNSLGRASMAA"
     gene            complement(7205..8017)
                     /locus_tag="PK513_06810"
     CDS             complement(7205..8017)
                     /locus_tag="PK513_06810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO82195.1"
                     /translation="MFSDNGLVFQKRLHLFEFCDQSWFAGWMREGFMDCLSLFYRVFR
                     PYHPFAQQIADDLRCGVICDLATGGGESIEFFLKYLEKKRADTRVKIIGTDLCPDVAS
                     LEVLKERYKCFDYTEHSVDALALPDNADNCDYTMFTAFHHFKEDRAVMLIRSVLEKGR
                     SLSVFEFTSRYDPLSYIFLLFGVPFSMLAPFFAKKRRWQKFLFSFIPVIPLIVAYDGF
                     VSNLRTYTKEELTILAQNAFPDQRVAVEFFEKRVLIVFKCYMCRFSIQHDDI"
     gene            complement(8046..9074)
                     /locus_tag="PK513_06815"
     CDS             complement(8046..9074)
                     /locus_tag="PK513_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOO82196.1"
                     /translation="MNQDPNYPRFGQQPPHQYGLHSFDTPSARRGIVEKLLGERIAQK
                     FSSPLFATAAFVIAGVAFAGVIIAVYPDSPDQNDIPVVKAETLAYKEIPSDPGGMSIP
                     NRDSTVFSAMSGEGPSEPAPLEDLLAEEEPVDKLAAFARQVEEAVEEDRVVGEESETA
                     PVTLQKIAKQSEDVASEAAGREVAQVVAPKPPPPVDDVERRKIIHKPGENPETLDFVR
                     SVLDKKDGQAVASANDVATRAASVAPAAGNAAREVGRDFNITPGSYYVQLGSVKSLSG
                     AEAEWGKLKKAFDAELGSASHRVQSADLGERGTFYRIQAGPMSKASAGEICDSIKAQK
                     PGGCLVTQ"
     gene            complement(9127..>9594)
                     /locus_tag="PK513_06820"
     CDS             complement(9127..>9594)
                     /locus_tag="PK513_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOO82197.1"
                     /translation="YGDLNSHVLVQEMIREMIGAMVEDVLEETESRLQDSNPQSPDDI
                     RAAGRQMVAFSEDMREKVDELRGFLFERMYHHYTMNRIHLKVERIICDLFTAFTKNYE
                     VLPNNWQRRVREAGGIENEQIRVRIVADYIAGMTDRYAIREHERLFDLYWDLK"
BASE COUNT         2761 a         2887 c         1779 g         2167 t
ORIGIN      
        1 ccctgccaga atgctcgaca cctgcaccgg catcgcttca ttttccgcca cagccttgcg
       61 caccttaggc gacgggtcct tttccgccag ataatacagg atttccttat tggtcttggt
      121 attcgaagcc agagacaacc gctctttcac attcgccgag gccaacgccg cgcgctgctg
      181 ctcatagctt ttcggcgttt tgccaaagaa tttactgaac ataaaaatca tacgtcaagt
      241 gtaacgaagg cgaacaaaaa tgcattagat ttttgattga cataaaataa aaaaatatgc
      301 tttaaaacac aaaaccataa aaataaagga actcaaaatg tcagcataca caaataaagc
      361 aggctcaaga gtaagaggtg catttaatgc caaaagtata aaacacaaca cacctctatc
      421 ggtgtacatt tctcctaaaa taaataaatc aacatctgac gacacaacag aaagaaggaa
      481 acaatatcaa gtaagctttg ctgctgcgat tacaagaatt agattacgtc tcaatgagct
      541 aggcatccgg gaaaacacca cagctaatac gccatctcat atcgccgtct ttgtcaacaa
      601 atcacaatta caaaagttag ctgaccaccc cgatattcag aaatttaaaa tgaatcctac
      661 agatgaaggt cacgtcgaag gaagaactat aaacttgatt taaaatcagc tttatccgcg
      721 cctcaaatac tctgcgcttt gcatctcctg caaccgcgat accgtgcgtt taaattcaaa
      781 cgcgtaatca tgccctcggt aaagttcgtc aggcagggca tcagccgtca tcaccaactt
      841 cgtcccggcc tcataaagcg tatcaattaa aatcataagc cgcttggctt cattgcgccg
      901 gtcgtaaccc agcgtcggaa ccccctcgac aaacaccgta tggtaattgt tggtaatctc
      961 cagataatcc tccgccccgt gcggctgttc acacaactgc gcaaaagaaa atcgtgcaac
     1021 gccacgcgca accgcatcca cccgaattgt acgccccttg acctccaaca tatccgtatc
     1081 aggcaaagca ttatccgtta gaccgttaaa caccgtattg gcatgacgca tcgtctcatg
     1141 gcccagcggc cagaaatacg tcccttcctg cgccaggaat ttagtgcgat aatccgtcga
     1201 gctatccagg tacaaaactt ccattttttc tttaagcagc gcaataaacg gcaaaaaacg
     1261 atcgcgctgc aaaccgccct cataaagacg atcaggctcc cagttactcg tcgccaccac
     1321 caccacgcca cgctcaaaca aatggcggaa gagccgcccc aaaatcatgg catcggcgac
     1381 atcggtcacg tgaaattcat caaaacacag cacccgcgcc cgctcgacaa tccgctctgc
     1441 gaacagcggt aagatcccat ccatcccctc gcacacatcc gcgctattgc gccgcgcatg
     1501 gtgataatca tgcaattcaa gcataaaggc atgaaaatgc acccgccttt tcggaatgct
     1561 gttgggcaag caatcataaa acaaatccat cagcatcgat ttgccgcgcc ccaccccgcc
     1621 atacatatac aacccgcgcg ccggacccgg cgccgtattc aaaaacggaa ttttctcctt
     1681 aatagcccgc cacccccggc aagccgcaga atgctcggaa atctccacca actcccaata
     1741 aagccgctcc agcgacttca cggccctttg ctgtgcagga tcaggcttta agatgccttc
     1801 atcaagacgg ctctgataga gtttttgcgg agcttttctt tgcggcatga cggataagaa
     1861 aaattgaaag ttcgtacagc cccaatattg gcacagccag cagaatttgg ctaatcacat
     1921 ccggcggtgt gataaaagca gccacaataa atgtaatgat cagcgcatat ttgcgtttag
     1981 acgccagcca ctcagcgctc accagccccg ccttccccat cagcgtcaac gcgaccggaa
     2041 gctgaaaaca caggccaaac gcaaaaatca gcgtcataat caaatcaaga tactcactca
     2101 cccgcgcctc aagctgaatc ggcagcgcgg tttcctctgc gctcgtctgg aaggacagga
     2161 aaaacggcca ggccatcggc aacacgatat aatacaccac cgcccctccg gcaaaaaaca
     2221 atatcggagt cgccaccata aacggcagga aagcctttcg ttcattgtca tacaaacccg
     2281 gcgcaataaa cccccagatc tgcaccagca aaatcggaaa ggtcaggaaa atcccggcga
     2341 aaaacgccac cttcagatag gtaaaaaatg cttccgtaag gccggtataa atcaggcgct
     2401 ggctatcacc ctcgcccatc gccccggcca aaggcgccac cagaaaactg taaatctgct
     2461 cgacaaaacc aaaacatata cctgtgccta aaatcatcgc accaaacacc cacatcaagc
     2521 gctgacgcaa ctcaatcaaa tgatgggtca acggctgagc ggtgttttca gtcatttttg
     2581 tatactcccg tcattgcgag cacagcgaag caatccaact ccgcagatgg attgccacgt
     2641 cggcctttcg gcctcctcgc aatgacgatt atttagcttc actttaaatc ttcctgattc
     2701 tgtttcaaag gcttcatcac gtcctcatct tcggcagcct cgtcgaaatc ggatgaccca
     2761 tcatgccgca cttcgaaatt caccgcctcg ttcagccctt ccgcatccat aaactcatca
     2821 aattgccggg aaatcgaata tttcatatat tggaaacgcc ggaacaaccg ccccatgccg
     2881 cgcatcaggc cgggaatttc ctttggccca agggcaaaca ccaccacagc catcaccaaa
     2941 aacaactctg cccagccaaa atcaagcata tcagcctaaa cggatctcca tatttgtact
     3001 atcggcagta aaccctgcct cctcaaacaa agcaacgctt ttagaatcat taaccggaat
     3061 ctccacaagc atatgttcaa tccccttatc tgtagcccac gcctgtgctt gcttcaacaa
     3121 actcggattc atttttgcac cactgccggg gcgaaaaaga acatgcgcct ctctttcaaa
     3181 attcgaccaa cttaaagaag agcgcttttt agacacgaca gcaatagctt cattcggctc
     3241 gaacaccgtg cttccttcaa aacccatcac atgatgcgaa tcaaaaacct tattattata
     3301 accaccaaaa ttaatgcttc tgggactaaa gccaaccgcc ttataaaaag caaggctcac
     3361 cttattttta ttcacgatct tcgtatatag agattcatgc ccggcattct cagcccagcc
     3421 tttagccgcg tccaataacg cagtcccgat tccttcttct cgatatttat caggaaaaac
     3481 aacaatatta tccagataag cttccgtaac gcgcgccctg ctaccaggaa atttttttgt
     3541 ctccggccac gcgaaaacag caccgaccgg agtcccttcc cgctccgcca ctttaatgca
     3601 gtaacgattc tcaacatagg aacgaagacc gggaacttcc cgccccaaag ccagcgcatt
     3661 aaactgccag cgcggcgcaa tttcgtccac ttcccggtat aaacccggct ccattttgca
     3721 atgatcacga tttacttgcg caaacgctgc acacagaaaa tcataatcaa atttaaccgc
     3781 atcacgaatt atcgtactta taagcttact cctaatataa aaaagctagc tcgataaagc
     3841 acaaacacaa aaaaacatca ataaaatatg gaaatttcaa tcatcttttt tagattgatc
     3901 gtcgatcgac ttgggtacgt catcatcgtc tttaagcccc tgcttaaagc tcttaattcc
     3961 cttggccaga ttttcggcaa tcatcggcaa acgggccgca ccaaacacaa ccaagatcaa
     4021 caagacaacg atcagaattt gtaacggtcc cggtgccatg gatttaaatc tcctctaatt
     4081 cctcttcggc gcgttcctgc tcgtaatcag cctcggcctt ttccagcgct tcgcgctcaa
     4141 tctcgcccgg ctcatccgca tcctgttcta cgcggctcaa agcctcttgt ccagcaaaca
     4201 gatcatcact gcccggaatg gcctcaatcg cagggcggcg atccagcagg cccgaggcct
     4261 tcaaatcatc cagccccggc agatccatca ccgattccaa agagaactga tccaaaaacg
     4321 cattcgtcgt cacccacgtc aaaggccgac ccggcgtttc acgccgccgt cccggcttca
     4381 cccagcccgc ctcaatcaag gcatccagcg tcccgcgatg cgtcgccacc ccgcgaatat
     4441 tctcaacttc ggcccgcgtc accggctggt gataggcaat aatcgccagc gtttccagcg
     4501 ccgcacgcga cagctttttc gaaacctctt tctcaatcgc caacgcctcg gccagatcgg
     4561 cagacgtgcg aaacgcccac ttaccgtcaa tctcaaccaa attcaccccg cgcccttcat
     4621 aatcgcgctt caggctcatc aacgccgccc cgacatccgc accatccggt gtatattcat
     4681 gcaaatccgc cgtagccagc ggctcggcac tggcaaacaa caacgcctcg acgatgcggg
     4741 taagttcatc catggcaatc attcggagta tgacttcatc gtcgcaaatc catcacgcat
     4801 ttcatgacgc atcggcttaa gctctgcaat atcgctttgc agctttttca gcacctcata
     4861 aagcaattca ttcgtaatct gagccatatg tcaaactcct gttttcatca tagcgtaaac
     4921 ttgacttatt catcatccgc gaccacgccg cgcatataaa tcggacgaaa cagcccatct
     4981 tgtttatata tgaaaatggt ctgttcaaaa tttatattca gagagataag cataaaacta
     5041 caactccgca aaagggagca aaaataaatt tatatcctct gctcatagac atttcatttt
     5101 cttgctttgc aacaagcaaa actcctttaa tacttaaaaa taaaactatt aaacttacta
     5161 ctatcaacca tatccatctt aaatagaaaa tataatttgg tgtataaaga acatcactgt
     5221 agatcccgtc aataaaataa attacacata tataaaaaaa gaataagttt attagagaaa
     5281 cgataatagg aattactaat gttattataa ttgatgttgc tttgttcatc ctcccccccc
     5341 cttcaaaact tgcagccatt aactgattat gcaaacctac gctttatccc ctgcaataac
     5401 gccgcgcata taaatcggac ggaacagccc atcctgctta atctccagtt tgccttgctt
     5461 aaccagctcc agcccggccg taaaggtcga ggccagcgcg ctgcgcccaa acagcttact
     5521 tctaacccct tccggcataa agctttccag cgtgctccac accgaatgca tccccttacg
     5581 cggcaattgc ccgagcatcc ggctcagccg tgacatcgcg ttttccatgc tcatcaggtt
     5641 aaaggtcggc aaatcgtaac tttgtccttc aatccgcttt ttgatatcgc cataggcttt
     5701 cagcaaatca tacagcttca catcccagcg cgtagtcgta tgcacccgca ccccttcggg
     5761 cgcgccgcga gcaaagacct gctggccaag ctgtggctgg cctatcagcc tatccgccgc
     5821 attttgcatc gcctcaaggc ggcgaagctg gaattgcaaa gcctcagcca tttcttccgc
     5881 actcggatca acctcattgt tttcacgagg taaaagcagg cgcgatttca gataagccaa
     5941 ccacgccgcc atcaccagat attccgccgc cagatcaagg ttaagttcct tggctcgctc
     6001 cataaagctt agatactggc gcaccaactg caaaatcgac acttgcaaca gatcaacctt
     6061 ttggttgcgc gccagctcca acaaaacatc gattggccct tcataaccgt cgatattcag
     6121 caacaatgca tccgcctcgc tcaccttttc ggcacgcggc gggtcttcct gaaattcgga
     6181 attttgaatc gcaatgtcgt tcatgccgcc atactggcgc ggccaagcga attttgcaag
     6241 cgtttgcgcg ccttgcgcga gaggttaggc acagttttta cgatcttttc catcaccgcc
     6301 aaatcgcctg cgcaatacaa agccgcatca caccccgcct caatcgtgag cgccgcacga
     6361 gctgcaatcg gcccataagc gcccagcgcc tccatatcca ggtcatcgga aaccaaaaac
     6421 ccatcaaaac caatatcttg ccgaatgatc ttctcaataa catctgaaga aaccgatgcc
     6481 gggcgttcat tatcgatctg atcataaaca atatgcgcta ccatcgccca aacacgacac
     6541 ccaacatcac tttcaacaac gatacgaaac ggatcgaaat catttttttg tagagtttcc
     6601 aaactttcag caacgtgcgg taagtccttg tgactatcca cttttccgcg cccatgccct
     6661 ggaatgtgtt taatcaccgg cataatcccc gcagccagca aatgcctaca tacagaaagc
     6721 ccaagccgtc caacaacttg gccgtcattg ctaaatgccc gatcaccaat cgcctcatgc
     6781 gtgtcatcct gcaaaacatc cagcaccggt gcgcaattca cattcacccc gacctcaacc
     6841 agttcctcag ccaactgcaa aatcgtaaac cgtaaatcct ccagcgcccc gtccatatcc
     6901 tcccgcgcct tgtcaccaaa gcttttcatg ggaggatact gccgccacat aggcggcttc
     6961 aggcgctgca cccgcccgcc ttcctgatca atcaggatcg ggcaatgcca gcccacgcaa
     7021 gcgcgcaatt catcacacaa agccttaacc tgccctggac tctcacaatt acgcacaaac
     7081 aggataaacc cgaccggatg cgcctcttta aaaaacgcac gctcatcccc cgacaagact
     7141 gcgcccgcac acgacaaaat caccgctttt atcaaatcga aactcttcgc ctcttaccca
     7201 taacttagat atcatcatgc tggatagaaa aacggcacat atagcattta aaaactatca
     7261 gcaccctttt ctcaaaaaat tcaacagcca cgcgctgatc tggaaaagca ttttgggcca
     7321 aaattgtaag ctcttcttta gtgtaggtac gcaagttact aacaaaacca tcatacgcaa
     7381 cgataagggg tataacggga atgaaagaaa acaaaaattt ctgccagcgc cgctttttcg
     7441 caaaaaaagg cgcgagcatg ctaaaaggca ccccaaaaag aagaaaaata taactaagtg
     7501 ggtcgtatcg cgatgtgaat tcaaaaacac taagagatcg ccctttctct aagacagaac
     7561 gaatcaacat caccgcacga tcctccttaa agtgatgaaa tgccgtaaac atcgtataat
     7621 cacaattatc agcattatca ggaagagcaa gcgcatcgac agaatgctct gtgtaatcaa
     7681 aacacttgta tctctctttg agcacttcca aggacgcaac atcaggacaa agatctgtcc
     7741 caataatttt aacacgggta tcagcgcgtt ttttctccag atattttaaa aaaaactcaa
     7801 tagattcccc tccacccgta gccagatcac aaataacacc acagcgtaaa tcatcagcaa
     7861 tttgttgtgc aaaaggatgg tacggtcgaa aaactctata aaatagggaa aggcaatcca
     7921 taaatccctc ccgcatccag cctgcaaacc aagactggtc acagaattca aacaaatgta
     7981 accgcttttg aaaaacgagg ccgttatcac taaacattat acctccattc tgcccaaaga
     8041 tgttattact gcgtcaccag acacccgccc ggtttttgcg ctttaataga atcgcaaatc
     8101 tcgcccgcac tggccttgct cataggcccg gcctgaatac ggtagaacgt cccacgctca
     8161 cccagatcag ccgattgaac gcggtgagac gcactgccaa gctcggcatc aaaagccttc
     8221 ttcaatttac cccactcggc ctcagccccg ctcaaagact tcacactccc aagctgcaca
     8281 tagtaggagc ccggcgtaat gttaaaatca cgccccacct ctcttgcagc attcccggcc
     8341 gcaggagcca cagacgccgc gcgcgtcgcc acatcattgg cgctcgcgac agcctgaccg
     8401 tctttcttat caagcacgga acgcacaaaa tcgagtgttt cagggttttc acccggcttg
     8461 tgaataattt tacggcgctc tacatcatca actggcggtg gtggtttcgg agccactacc
     8521 tgcgcaacct cacggcccgc agcctccgaa gcaacatctt cactctgctt ggcaatcttc
     8581 tggagggtta caggcgcagt ttcgctctct tcgcccacca cacggtcttc ctcaacagcc
     8641 tcttcaacct gacgcgcaaa cgccgccagc ttatcaacag gctcttcctc agccagcaaa
     8701 tcctcaagcg gcgcaggttc agacggacct tccccgctca tcgcagaaaa aactgtactg
     8761 tcgcgattag gaatgctcat cccgcccgga tcgctaggaa tttctttata ggccaacgtt
     8821 tccgctttca caacgggaat atcattctga tcaggcgaat cgggatacac ggcaataatc
     8881 accccggcaa aagcaacgcc cgcaatcaca aacgccgcag ttgcaaataa aggactggaa
     8941 aatttctgcg caatcctttc ccctaaaagc ttttcaacaa tacctcttct cgccgacggc
     9001 gtatcaaaac tatgcaaccc atactgatgt ggcggttgct ggccaaaacg tgggtaattc
     9061 ggatcctgat tcatttttcg tccctcacaa aaacacagtg attcttaaat ttaagctgct
     9121 caaagtttat ttcagatccc aatacagatc aaataaacgc tcatgctccc gaatagcgta
     9181 acggtcagtc atcccggcaa tatagtcagc cacaatccgc acacgaatct gctcattctc
     9241 tatgcctcca gcctcacgca cacgccgttg ccaattgttg ggaagcactt catagttttt
     9301 cgtaaaagct gtgaataagt cacagataat ccgctcgacc ttcaaatgaa tccggttcat
     9361 cgtataatgg tgatacatcc gctcaaacaa aaacccgcgc agttcatcaa ccttttcacg
     9421 catatcctcg ctgaaagcca ccatttgccg ccccgccgcg cgaatatcgt ctgggctttg
     9481 cggatttgaa tcctgcaagc gcgattcggt ctcttccaaa acatcctcaa ccatcgcgcc
     9541 gatcatctcg cgaatcattt cctgtaccag cacatgactg ttcaaatcac cata
//
LOCUS       DAOSWR010000079         9367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4679_length_9367_cov_6.092461, whole genome shotgun sequence.
ACCESSION   DAOSWR010000079 DAOSWR010000000
VERSION     DAOSWR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4679_length_9367_cov_6.092461"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1078)
                     /locus_tag="PK513_06825"
     CDS             complement(296..1078)
                     /locus_tag="PK513_06825"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOO82198.1"
                     /translation="MSMFPALKKTQEKIEKIEESLNPAVKKVLALLRLDTASLKNEKA
                     GLSAFETVLDHHRIVSHRTRSKDADTFRFLRTQILQSMNEHGFKTVAISSPRYGDGKT
                     TIAANLAVSIAQDLKQTVLLVDLDLRKPSLAEYLELSPKAGLTDYLAGKADVKDCLIR
                     MPFERINVFPAGRPVDRSSETLGSPQMQKLAEELKERYDDRLIIYDMPPLLEQDDPLV
                     FLPHVDAFLLVIREGVTTVDEIKRSLDILKSARVIGVVLNAV"
     gene            complement(1129..2844)
                     /locus_tag="PK513_06830"
     CDS             complement(1129..2844)
                     /locus_tag="PK513_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82199.1"
                     /translation="MSAFYPLEYLSVLKRQKLLFFTIFGSILFVSFLFVAQWSKYRAF
                     ATVEVVPPEIAIDAVESPQNRILTAEAMADLQISRLKQKVLSTSSLADIVTKLNLYPK
                     ARAHTPIAYIAKNMQRKIDIHLMSTVLANPASAQKVSAFQLSAIAFTLSFEYSDPFLA
                     QKTVNELVSRFLDEDLKDRRETAQKTSEFLEGQIEILSATLEEQEQKIAEFQAAHGVT
                     RPDALGFNQQASITTSSRLLAIESELISNLGLIGALRAQLVQTDPYSRVVDEGGEVLS
                     TPAIQLRALKSQYATLSAKYGPAHPDVLKVSRQINALKNEISPVNLSARLKAKITDMK
                     AKLSSAEKTYGSAHPDYISLQNQLQNLEDQLENVQNDAASLVKDDADNPAYLQIVVQI
                     NAAEKQREALEAQRDAVKSQQEEFRKAVADNPVAEQKMAALARDYDNMMFLYRDLKAR
                     KLAADMSKTIEEGHVGRRLAVIDPPELPLGTSPSKKLILAGGFVFALMAGLGSIIGLQ
                     ILSQTVIGPHHLESLIGSSPLITVPHLFSLEEKIKARRLIIKLLVVAPFVAAVPLILI
                     SFKPL"
     gene            complement(2888..4069)
                     /locus_tag="PK513_06835"
     CDS             complement(2888..4069)
                     /locus_tag="PK513_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82200.1"
                     /translation="MQIFSAVPMIRCALASFLMGGFVLPYGLTPAFAKDNVKIGISET
                     PLWDSNPLMLTTGKTDIWGSETRAFVDFGRMTPDATLNATLAAEHNYFNNSSFNSNDF
                     FFRTDLKKNTALWEWSINGRFDYDTTRTGEITTFGRNTRVGRRLSWQAAPRVAYNLSP
                     RAQLVLDGSWQENHYDNSVSLTDYRTVSLSPSVVYSLTPLQKAFLLFRTQHYSSLDGD
                     QDVDSLGPSLGWQYRFKPEWSLELFAGFLASKYDGYVTAGNDWEYNPTYSATLEYLGQ
                     RNHTSFSVARARQSYANGTESELISATAENKFTINSQWALSMKALYQDAKRSTVSMGN
                     LDTGYEESVGLVYKAGERWDFSLSQRFRREELTNGLGVAERNIIRAGLTYRLGAVPYL
                     Q"
     gene            complement(4074..4655)
                     /locus_tag="PK513_06840"
     CDS             complement(4074..4655)
                     /locus_tag="PK513_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOO82201.1"
                     /translation="MNIMIKIVCLGSLFLTFLSPHPVIALGDDFVILPGDVLHINVWK
                     EDGMDQDVLVLPDGTITFPLIGTLKARDMSPAKLQAAIRDKLNPFIPDAAVTVSVQAP
                     TGHKVSLVGQLREPGEVVLTARMSVMQAISQVGGLTPYADEDDIIVIRENADGEKEKI
                     SIPYNAITKGRDLEKDISLLPGDVVVVPASGLF"
     gene            complement(4804..5307)
                     /locus_tag="PK513_06845"
     CDS             complement(4804..5307)
                     /locus_tag="PK513_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HOO82202.1"
                     /translation="MGTVVPGTAGSFTFSGGGVTGLWVAGDVFGKLPPLSMVEFTYNS
                     TGPFLFQTLTSAGQYNGGLEYSFENVPSGAAGSSAGSLVITSAQILSSQEATGIIKNL
                     SSEIADFQSVFAGFFFGAGKQLNIEYSVSAVPVPAALPLFVMGLGGLVFGARRKKKAQ
                     VDSLVVA"
     gene            5640..7973
                     /locus_tag="PK513_06850"
     CDS             5640..7973
                     /locus_tag="PK513_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO82203.1"
                     /translation="MSCDNLEEKYEKHLGRGDSYYEKQDYIKARLEYKNAAKILPTDP
                     RAIYSLGLVEEAEGSLQKALAAFWVTEQQNPDFKPVLLKLAELYLTAQQSKEARTRID
                     KLLKLEPDHVGAHALMGSIFLEEKNFPQAHKEVKFVLQKDPSNMVAYSVLTGIYVAKK
                     RPNKALEILEKAIALHPKEKSFYLLKAMLYSEQNDIAAVARTYRKIFMLYPEQIRFRF
                     DLARIFEKVEQTDNAGKVYEETVRVFPKNQEAKQKLAMFLENEKGMDAAERKIHTFIE
                     AEPNQKLFYLWLAGLYVRNEQDERAIATLKNIIKDAPDDWIGLNAATTLAEIGLSKGD
                     IELTEKLIGTVLNKDVNNREALLLQANLAFFQKDYQKAAVDLRVILRDEPGAIKASRT
                     LAEIFSIQKRNDLAIDTLLLAVQKHPGDKGIQVRLAQFYALTNNRDKAMELLSTVTNT
                     APEYPIGWETLARLAIENGQWAQAHNAASKLNKLEGQRNTATFLKGQISEKTGQIQEA
                     IALYKDVIKADPSSPLTVHALSALLKTSQTPEQLKAFRDFLITIEPQNFTLLTVLGAT
                     ESALGDSEAAKTAFKTAINQNPRNQEPYIALAKILVQQGTPEGAIDILKKAQTDIPSE
                     TEASIMHAGLLIKNERIDEAIALFEAILKRNERLDNAANNLAQTIADFKYDDETALER
                     ARLVAERFINSENPYYLDTLGWVYFQQGHIPQAQPLLSRAVSLLKEPNPQIDYHYGAL
                     LFKTGQKEDARTYLSRAVSGPQYPGFDKAQALLKEIN"
     gene            8146..8481
                     /locus_tag="PK513_06855"
     CDS             8146..8481
                     /locus_tag="PK513_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82204.1"
                     /translation="MPTILPKDADNIPLPALRLKDNAAHAINVTASSARNINAFDTET
                     KIVSLYATGPVYLKFGGASVTATTSDHYFPEGVYYDISIGDDKTGHLSHVAAIRASSD
                     CQLFISEKH"
     gene            8504..9271
                     /locus_tag="PK513_06860"
     CDS             8504..9271
                     /locus_tag="PK513_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82205.1"
                     /translation="MPSQPPLPIGKIGKAGISNSASLSPTSSPFSPLNLSPVLWFDAS
                     DVSTITQSAGAVSQWDDKSGNNYHVTQGTATNQPTTGTRTLNSLNVLDFDGTDRLLAS
                     VSDYPETATVLIVGEVDTLGTSGFSRSLFSIDGAADYQEEAGNSTQYLFQINSTQHTN
                     IKQGTTDLIGSPHIFQYVHDLDTNSAAVYVDGVAGGSANNYNTAYSATQSLQVGANRA
                     QNTPHIGGISEIIIYDKVLTAQENTDVLDYLTNKWGL"
BASE COUNT         2899 a         2297 c         1978 g         2193 t
ORIGIN      
        1 gatcgtagct taacttcgcc gcaaagctgg ccgaagaacc gagaccacct ctaacgaaac
       61 caatagcgtt ttaaaaacga actttaaacg ccgcaccgca ccggatgaaa aaatttttat
      121 caggagctgg aaagctatat ataatccaga tccagattat ataccaaaac ctcatccgga
      181 taagatacgg tacgtagcag aacaagaaca gacaccgcca atggaagctg tttttcaggt
      241 tgcctaatga atacacgcca ccactacaga tcgcgactga ccagataaat gaagcctaca
      301 cagcgttcag cacaacccca ataactctgg cggattttag aatatcaaga ctgcgtttaa
      361 tttcatcaac ggtggtcacg ccttcacgta taaccaaaag aaacgcatcg acgtgaggca
      421 aaaaaaccag cggatcgtcc tgttcgagca aaggaggcat atcgtagata atcaaacggt
      481 catcataacg ttccttaagt tcttcagcca gtttctgcat ttgcggtgag ccaagcgttt
      541 ctgacgaccg atcgacaggc cgcccggctg gaaacacatt aatacgctca aacggcatac
      601 ggatcaggca atctttgacg tcggcttttc cagcaagata gtctgtcaac ccggccttag
      661 gagaaagttc cagatactcc gccaaagacg gcttgcgcag atcaagatcg accagcaaaa
      721 ccgtctgctt taaatcctga gcgatactca cggcaaggtt agccgcaata gttgtcttcc
      781 cgtcaccata gcgcggagaa ctgatggcaa cggttttaaa gccatgctca ttcatgctct
      841 gaagaatttg tgttcgcaaa aaacgaaaag tatcggcatc cttactacga gtcctatgag
      901 aaacgatgcg atggtggtcc aacacggttt caaatgcaga cagccccgct ttctcatttt
      961 taagcgaagc agtatcaagc cgaagaagcg ccagcacttt tttgacggca gggttgaggc
     1021 tttcttctat tttttcaatt ttttcttgcg tttttttgag ggcaggaaac atggacattc
     1081 tcacattcgt aaatcagtgg gttataatta cactccggat tttccgttct ataacggttt
     1141 gaaagatatt aaaatcaaag gaaccgccgc cacaaaaggg gccacaacaa ggagcttaat
     1201 gataagacgc cgggctttta ttttttcctc cagggaaaaa aggtgcggaa cggtaataag
     1261 aggtgaacta ccgatcagag attccagatg gtgcggcccg atcacagttt gagatagaat
     1321 ttgaagaccg atgatactgc ccaaccccgc cataagggca aaaacaaacc cgcctgccaa
     1381 aatcagcttt ttggaaggac tggtcccaag aggcaactcc ggaggatcaa taacggcaag
     1441 acgccgacct acatgcccct cttcaatggt cttactcata tctgccgcca gctttctggc
     1501 tttaagatcg cggtacaaaa acatcatgtt gtcatagtct ctagccagag ccgccatctt
     1561 ttgttctgca acggggttat cggcaacagc ctttcgaaat tcttcctgtt ggcttttaac
     1621 cgcatcacgc tgcgcttcca gggcttctct ttgtttttcc gcggcgttaa tctgcacgac
     1681 aatctgcaaa taagcagggt tatcagcatc atccttaacc agactggcgg catcattttg
     1741 aacattttca agttgatctt cgagattttg aagctggttt tgcagagaaa tataatcagg
     1801 atgtgcactg ccatatgttt tttccgcact agaaagtttt gccttcatat cggtaatttt
     1861 agccttgagc cgggcgctta gattcaccgg cgatatttcg tttttgaggg cattaatctg
     1921 ccgcgaaacc ttcaagacat ctggatgcgc gggaccatat tttgcactca acgttgcata
     1981 ttgcgatttc aaagcccgca gctgaatagc aggagtactt agaacttcac caccttcatc
     2041 aacaacgcgc gaatagggat cggtttgtac aagctgggca cgaagagcgc cgataagacc
     2101 gagattggag ataagctcac tttcaatcgc caagagccgg gaagaggtcg taatggaagc
     2161 ctgttgatta aagccaagag catcaggacg cgtcacacca tgcgccgcct gaaattctgc
     2221 aattttttgc tcttgttctt caagcgttgc tgacaatatc tcaatctgac cttcaagaaa
     2281 ctccgaagtt ttttgcgccg tctcacgacg atctttcaga tcttcatcaa gaaaacgtga
     2341 aacgagttca ttgacagttt tctgtgctaa aaaaggatcg gaatactcaa agcttagagt
     2401 aaaggcaatc gctgaaagct gaaaagccga gactttctga gccgatgcag ggttggcaag
     2461 tacagtgctc ataagatgaa tatcaatttt tctttgcata tttttagcaa tataagcaat
     2521 aggcgtatga gcacgggcct tcggataaag attaagttta gtaacgatgt ccgccagaga
     2581 actggtcgag agcacttttt gtttaagacg gctgatttgc aaatcggcca ttgcctctgc
     2641 cgtcaggatt ctattctgtg gagattcaac ggcatctata gcaatttcag gaggtacaac
     2701 ttcaaccgta gcaaaagccc ggtatttcga ccattgtgcc acaaaaagaa acgatacgaa
     2761 gagtatcgat ccaaaaatgg taaaaaacaa aagcttctgg cgtttcaaca ccgataaata
     2821 ttcaagagga taaaatgcgc tcatactaat cccgcgtcta aatgtccctt gtattacaca
     2881 agaactgtca ttgtaaatat ggcaccgcgc ctaaacggta tgttaaaccg gctctaataa
     2941 tattccgctc ggccacgcca agcccgtttg tcaattcctc ccgccgaaat ctttgtgaaa
     3001 gcgaaaaatc ccagcgctcg cctgctttat aaacaagacc aaccgattct tcatatcctg
     3061 tatcaagatt gcccattgaa accgtagaac gtttggcatc ttggtataga gccttcatac
     3121 tcagagccca ttgagaatta attgtaaact tgttttctgc cgtagcgctg atcagttcgg
     3181 attcagtacc attcgcataa gattgccttg ctcgcgctac ggaaaaggac gtatggtttc
     3241 gctgccctag atattcaagc gttgcagaat aagttggatt atattcccaa tcattacccg
     3301 ccgtcacata accatcatac ttcgaagcaa gaaaaccggc aaaaagctca agtgaccatt
     3361 ccggtttaaa acgatattgc cagccaagag acggtcccag actatcaaca tcctgatcgc
     3421 catcgagaga actatagtgc tgagttcgaa acaagagaaa tgctttttgt agcggagtaa
     3481 gactataaac aaccgacggg gagagcgaca ccgtccggta atcggttaaa gaaacactgt
     3541 tatcgtaatg attttcctgc catgacccgt ccagcaccaa ttgcgcacgc ggcgaaagat
     3601 tataggctac acgcggagcg gcctgccaac tcaagcgtct tcccacgcgt gtattacgac
     3661 caaatgttgt aatttcgccg gtccgtgtcg tatcataatc aaaacgaccg ttgatcgacc
     3721 actcccaaag cgcggtattt ttcttcagat ccgtcctaaa gaaaaaatca ttggagttaa
     3781 aagatgagtt gttaaaataa ttatgttcag cagccaacgt ggcattcaaa gttgcatctg
     3841 gggtcatacg cccgaaatcg acaaaagctc tggtttcaga cccccaaata tcggttttcc
     3901 ctgttgtaag cataagcgga ttggaatccc acaagggcgt ttcactgatg ccgatcttga
     3961 cattatcttt cgcaaaagcg ggagtaaggc catagggcaa aacaaacccg cccattaaaa
     4021 aactggccaa agcgcaccgg atcataggaa ctgccgaaaa aatttgcatg tgatcaaaaa
     4081 aggcccgaag caggaacaac aacaacatca ccaggaagaa gagaaatatc cttttcaaga
     4141 tcccggcctt tggtaatcgc gttataagga atagatattt tctccttttc accatcagca
     4201 ttttcgcgaa tgacaataat atcatcctcg tccgcatacg gcgttaaacc gccaacctga
     4261 ctgattgcct gcatcacact catacgagca gtcaaaacga cctcaccggg ttctcgaagt
     4321 tgcccgacaa gactgacctt atgccccgtc ggagcctgaa cactgacggt cacagccgca
     4381 tcaggaataa aggggttcag tttatcacga atggcagctt gcagtttggc cggactcatg
     4441 tcacgagcct tcaaggttcc gatcaaggga aaggtgatgg ttccatcagg caggacaaga
     4501 acatcctgat ccatgccgtc ttctttccag acattgatat gaagaacatc ccctggaaga
     4561 ataacaaagt catcgcccag agcaataaca ggatgaggag ataaaaatgt caggaaaagt
     4621 gatcccagac agacaatttt gatcataata ttcatacgtt actcactggt ttgaaaacag
     4681 gataacaacc aagtgccatg taagcgatta ttcgcataaa tagcaatcaa atatagcgat
     4741 tagcctcaat acaaaaaaga cgccacagag gcgtcttttt tctaaaaacc aaaaatttcc
     4801 ggatcaggca acaacaagac tatctacttg cgctttcttc tttctccggg caccaaagac
     4861 cagaccgcca agccccatca caaacaacgg caaagctgcc ggaacgggaa ccgcagaaac
     4921 actatactca atattgagct gcttgcctgc accaaaaaag aatccagcga atacactctg
     4981 aaagtctgca atttcagagg aaaggttttt gataataccc gtcgcttcct gggaagaaag
     5041 aatctgggca cttgtaataa caagagaacc tgcggaggaa cccgcagccc cggaaggaac
     5101 attttcaaat gaatattcca aaccgccatt atactggccg gcgctcgtca atgtttgaaa
     5161 caggaaaggc ccggtagaat tgtaagtaaa ttcaaccata ctcaacggcg gaagctttcc
     5221 gaaaacatca ccagcgaccc acagccctgt aacaccgcca ccagagaaag taaaacttcc
     5281 cgcagttccc ggaaccaccg tgcccaaatc ctcactatag gctgctgatg cttttccggc
     5341 actaaggcca aaacacaaaa caacgacagc gatcaacaat agacgacaag gcttcataaa
     5401 ccatccctca acaactcaaa aaaacaccaa actaagagct agcatgcagt tcacagtatt
     5461 acaacagatt tttataatgg aatataccat ggcatatcaa aacaaataaa aattctggaa
     5521 aatcatagca gactgctata ctaaacctgt tttacaagcc ctgcgcaact cggaaaattt
     5581 ttatgaaatc ttacaaatac gtccatacta tcttattgct tgtgttggga acgctcctta
     5641 tgtcctgcga caacctggaa gagaagtacg aaaagcatct cgggcgcggc gattcctatt
     5701 acgagaaaca agattacata aaagcgcggt tggagtacaa gaacgccgcg aaaatcctgc
     5761 caacggaccc gcgcgcaatc tacagccttg gacttgttga agaagcggaa ggaagcctgc
     5821 aaaaggcgct ggcggctttt tgggttacgg aacagcaaaa tccggatttt aaaccggtat
     5881 tactaaaact agcagagctt tatctaactg cgcagcaatc gaaagaagcc cgtacaagaa
     5941 tcgacaaact tctgaaactg gagccggatc atgttggggc ccacgccctc atgggatcta
     6001 tatttttaga agaaaagaat ttccctcagg cacacaaaga ggttaaattt gtcctgcaaa
     6061 aagacccttc aaatatggtt gcctattcgg ttctaacggg catctatgtt gcaaaaaagc
     6121 gacctaacaa agcgcttgaa attctcgaaa aggctattgc cctgcatccg aaggaaaaat
     6181 cattctatct cctgaaagca atgctttaca gtgaacaaaa cgacattgcc gccgttgcgc
     6241 gcacctatcg taaaattttt atgctctacc ctgagcaaat tcgtttccgg tttgatctgg
     6301 cccgaatttt tgaaaaagta gaacaaacag ataatgccgg aaaagtctac gaggaaaccg
     6361 ttcgggtttt tcccaaaaac caggaagcca aacaaaaact ggccatgttt cttgaaaatg
     6421 aaaaaggcat ggacgccgcc gagcgaaaaa ttcacacctt cattgaggct gagcccaatc
     6481 agaagctgtt ttatctttgg ctggcaggcc tttacgtccg caatgagcag gacgaacgtg
     6541 ctatcgccac cctcaaaaac atcatcaagg atgcccccga tgattggatc ggtttgaacg
     6601 ccgctacaac acttgcggaa ataggattaa gtaaaggcga tattgaattg acagaaaagc
     6661 tgataggcac ggtcctaaac aaagacgtca acaatcgtga agcgctttta ttgcaggcaa
     6721 atctggcttt tttccaaaaa gactatcaaa aagcggctgt tgatctcagg gtaatcttac
     6781 gcgatgagcc gggcgcaata aaagcatcca gaactctggc agaaattttt agtatccaaa
     6841 agcgcaatga cctggcgatc gacacactcc tcctggccgt acaaaaacac cccggcgaca
     6901 agggcataca ggtcagactg gcacagtttt atgctctgac aaacaatcgt gataaggcaa
     6961 tggagcttct ttccaccgtg acaaacactg ccccggaata tcccatcgga tgggaaacac
     7021 ttgcccggct ggcaattgaa aacggacaat gggcacaagc acacaacgcc gcgtctaaac
     7081 taaataaact ggaaggtcaa cggaacacag ccacttttct aaaaggacag atcagtgaaa
     7141 aaaccggaca gatccaggaa gccatcgcgc tttataaaga tgtgattaaa gcagatccgt
     7201 cctcgccgtt aactgtccat gcgctttccg cattactaaa aacatcacaa acacccgaac
     7261 aactgaaagc gtttagagat tttctcatca ccatcgaacc ccaaaatttt acattactaa
     7321 cggttctagg agcaaccgag agcgcgcttg gagattccga agccgctaaa accgccttca
     7381 aaactgccat caatcaaaat ccacgcaatc aggaacccta tattgcgctt gctaaaattc
     7441 tcgtacaaca aggcacacca gaaggcgcta tcgatatttt aaaaaaagcg caaaccgata
     7501 taccatccga aaccgaagcc tccataatgc atgctggtct cttgataaaa aatgaacgca
     7561 tcgacgaagc aatcgcgctt tttgaagcca ttctgaaacg caatgaacgg ttggacaatg
     7621 ctgcgaataa ccttgcccag actatcgcag acttcaaata tgacgatgaa acagctctcg
     7681 aacgcgcacg gttagtcgca gaacggttta tcaactccga aaacccttat tacctcgata
     7741 cattgggatg ggtatatttc cagcaaggac atatcccgca agcacaaccg ctcctctcaa
     7801 gggctgtctc tcttttgaag gagccaaatc cacaaattga ctatcattac ggcgcgctgc
     7861 tgtttaaaac cggacagaaa gaagacgctc gaacatatct cagccgcgct gtttccggcc
     7921 ctcaatatcc cggatttgat aaagcgcagg cgcttctgaa agaaataaac taaccttatt
     7981 tacaagcccc aagagttaaa aaaggaaaat attcctaaaa aatgcttgac taaagaacaa
     8041 aaaaagaaca tattaacaac acattttgag gacaattgcg tccacctccc ttgtaagggc
     8101 aggcggcttt ttgtctgtta acgtcagata aaggagaatg acaatatgcc tacaatactg
     8161 cccaaagacg ccgacaatat tcccttgcct gcgttgcgcc taaaagataa cgccgcgcac
     8221 gcaatcaatg taacggccag ctcggcgaga aatataaatg cctttgatac ggaaacaaag
     8281 atcgtcagtc tgtacgcaac cggacctgtt tatctgaagt tcggcggcgc atccgtgaca
     8341 gccacaacga gcgatcatta tttcccggaa ggcgtctact acgacatttc gatcggcgac
     8401 gataaaaccg gacatctttc tcacgtcgcc gccatccgtg ccagttcgga ctgtcaactt
     8461 ttcatttccg aaaaacatta atgccagaat taaggagagc gtcatgcctt cacaaccacc
     8521 tctaccaata ggaaaaattg ggaaagcagg catttccaat agcgcgtcgt tatcccccac
     8581 ctcctcccct tttagccccc tcaacctttc gcccgttcta tggtttgacg cttctgacgt
     8641 aagcaccatc acgcaaagcg ccggagcggt gagccagtgg gacgataaaa gcggtaataa
     8701 ttatcacgtc acgcagggaa cggcgactaa ccagccaaca acgggtacgc gaacacttaa
     8761 cagcttgaat gttcttgatt ttgacggaac tgaccgccta cttgcgtctg tttcagatta
     8821 tccggaaaca gcaacggttc ttattgttgg tgaagtagac acacttggta cttccggatt
     8881 ttcacgttca cttttctcaa tagacggagc ggcggattat caggaagagg ctggaaactc
     8941 gacacaatat cttttccaga ttaattcaac acaacataca aatataaaac aagggactac
     9001 agaccttata ggcagtccgc atatattcca atacgtccat gatctggata caaatagcgc
     9061 tgctgtgtac gttgacggtg tggctggcgg gagtgcaaac aactataata ctgcttattc
     9121 tgctacgcag agcctccaag tgggggcaaa ccgcgcacaa aacacaccgc atattggcgg
     9181 aatatctgag ataatcattt acgataaagt actaaccgcg caagaaaaca cagatgttct
     9241 agactatctt acaaataaat gggggcttta acatgaaaat attggaattt aatacactgg
     9301 gcgaggtgca aaaagaggtg gtctcggttc ttcggccagc tttgcggcga agttaagcta
     9361 cgatcgg
//
LOCUS       DAOSWR010000080         9237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4774_length_9237_cov_5.292420, whole genome shotgun sequence.
ACCESSION   DAOSWR010000080 DAOSWR010000000
VERSION     DAOSWR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4774_length_9237_cov_5.292420"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="rplL"
                     /locus_tag="PK513_06865"
     CDS             complement(<1..341)
                     /gene="rplL"
                     /locus_tag="PK513_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOO82206.1"
                     /translation="MAANLEKIVEQLSELSVLEAAELSKLLEDKWGVTAAAAAPVAVA
                     AVAGGDAAGAAEEKDSFDVVLESAGGNKIAVIKEVRAITGLGLKEAKDLVEAGGNIKE
                     GAKKDEAEEIK"
     gene            complement(379..891)
                     /gene="rplJ"
                     /locus_tag="PK513_06870"
     CDS             complement(379..891)
                     /gene="rplJ"
                     /locus_tag="PK513_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOO82207.1"
                     /translation="MSRAQKKTEIQSLNERFANDELVVVTHYSGLTVKEMADLRAGLR
                     SEGASFKVTKNTLAKIALKGTKFEAISDMFTGPTGVATSQDFVAAAKAAYKFAKGNDK
                     LIILGGALGELVLDAAGIEALAKLPSLDEVRSKLVGLLQAPATKLAGLMQAPARELVG
                     VTKAYGEKGE"
     gene            complement(1158..1862)
                     /gene="rplA"
                     /locus_tag="PK513_06875"
     CDS             complement(1158..1862)
                     /gene="rplA"
                     /locus_tag="PK513_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOO82208.1"
                     /translation="MPKQTKKMKAALEGVDRNKEYTLEDAVKALKSAAKARNFDETFE
                     IAINLNVDPRHADQAVRGMISLPNGTGSSVRVAVFAKDDKAKAAKAAGADIVGAEDLA
                     EKIKAGEMDFDRCIATPDMMVIVGQLGKVLGPKGLMPNPKLGTVTPDVEKAVKDAKGG
                     AIEFRAEKAGVVHAGVGKASFDDKKLAENIRAFYAAIIKAKPSGAKGTYVKKVSLTST
                     MGPGLKIDTASLSAAA"
     gene            complement(1866..2294)
                     /gene="rplK"
                     /locus_tag="PK513_06880"
     CDS             complement(1866..2294)
                     /gene="rplK"
                     /locus_tag="PK513_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO82209.1"
                     /translation="MAKKVSGYINLTITGGAANPSPPVGPALGQHGVNIMEFCKAFNA
                     KTEDSKGIPIPVVITVFEDRSFDFITKTPPASYFIKQATKQKKGSGAPGRDIIGTIRT
                     SQLKEIAEKKMEDLNANDIDAAIKIIAGSARSMGIDVVED"
     gene            complement(2415..2951)
                     /gene="nusG"
                     /locus_tag="PK513_06885"
     CDS             complement(2415..2951)
                     /gene="nusG"
                     /locus_tag="PK513_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOO82210.1"
                     /translation="MSAAKWYVLHVYSGFEQKVAEAIKEKARKQGLESHVEEIMVPTE
                     EVVEVKRGQRVNAERKFFPGYVLAKLDMNDNVWHLIKDTPKVSGFLGAGGNKPVPITE
                     AEANRLIQQVQEGIDRPRPSVVYDIGEEIKVTDGPFASFSGIVEEIDEEKQKLKVSVS
                     IFGRATPVELEYGQVEKV"
     gene            complement(2977..3168)
                     /gene="secE"
                     /locus_tag="PK513_06890"
     CDS             complement(2977..3168)
                     /gene="secE"
                     /locus_tag="PK513_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOO82211.1"
                     /translation="MASPVEFFKQVRSEAKKVTWPSRHETTVSTIAVFIMVFIASVFL
                     YFSDQIIAWIVRLIMGFGL"
     gene            3311..3481
                     /locus_tag="PK513_06895"
     CDS             3311..3481
                     /locus_tag="PK513_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82212.1"
                     /translation="MLSAIFKLSGLNEVLEASQEAVLPYLTRSHDITGVDGKKDSSGS
                     GPTLDIGAGSGD"
     gene            complement(3478..5019)
                     /gene="gcvPB"
                     /locus_tag="PK513_06900"
     CDS             complement(3478..5019)
                     /gene="gcvPB"
                     /locus_tag="PK513_06900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOO82213.1"
                     /translation="MSAENKIVETMDTKMNDDNRGLHYEENLLWEKERSDHPGVDLPA
                     PAGMPLRTGQQARENIGLPQVSEPQALRHFVRMSTWNYSIDHGFFPLGSCTMKHNPRL
                     NEKLARLPGFAHLHPLQPESTTQGALALMYELQQWLAELTNLSGVCLSPAAGAHGELA
                     GMMTIRRAHEQKGDGKKRKIVLTPDSAHGTNPATATTCGYEVRNIPTKDTGMLTVEAF
                     KKALYKTDPAGGDIAGMMVTNPNTCGLFEKDILEIANLLHKAGGYFYMDGANFNALVG
                     KIRPGDFGVDVMHINLHKTFSTPHGGGGPGSGPICCTEELGAYMPVPTVLKEGETYRL
                     ETEIDRLTNCGRLKGFHGQFGMHVRALAYMLSHGADGLKQVSEDAVLNANYILRSLEG
                     DYHAPFSAQGPCMHEALLTDKKQKECGVQTLDIAKALIEKGFHPMTVYFPLVVPGTML
                     IEPTDTESKDSLDRFITAMKDIAGEAEQRSDAEDHFHSFPHSTPRRRLDEVKAARNPV
                     LRWKN"
     gene            complement(5016..6296)
                     /gene="gcvPA"
                     /locus_tag="PK513_06905"
     CDS             complement(5016..6296)
                     /gene="gcvPA"
                     /locus_tag="PK513_06905"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOO82214.1"
                     /translation="MRYLPQTQNARAAMLKTIGVGSIDDLYVDVPATEMGHKFNLPDH
                     QGELDVERHLNAYANQNHAASAGPFFLGAGAYYHHIPASVDYIIQRSEFLTAYTPYQP
                     EIAQGTLQAIFEFQTFIAQLTGQDVANASMYDGATATTEAALMALRVTKRKKIVIGNA
                     LHPDYRGVLKSYMWNLDGDVKITDGAPDEQTACVIIQSPDFYGAPHKYDALRKSCDET
                     GALLIVVVTEIVSLGLLPAPAEADIVCGEAQSIGIPLSYGGPYLGFFACKEKYLRQMP
                     GRLCGMTEDADGKRGFVLTLSTREQHIRRDKATSNICTNQGLCALAFTVHMSLLGEDG
                     FKRLARLNHEAACRTADALTQIPGVKLLTENFFNEFTLELPRASKGIVKALAGQGIIA
                     GYDLDENQLLIAATEMTTDEDIAALTTAMKEALA"
     gene            complement(6396..6773)
                     /gene="gcvH"
                     /locus_tag="PK513_06910"
     CDS             complement(6396..6773)
                     /gene="gcvH"
                     /locus_tag="PK513_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOO82215.1"
                     /translation="MAELKFTTDHEAIYVDGDLAWVGITEYAREALGDLVFVELPEIG
                     KQVSKGDEFAVVESVKTAAEVYSPIDGEVVEANDNMPDDLELIAKPLADGGWIAKIKI
                     TDPAQLGDLMDEATYKDYLKTLD"
     gene            complement(6766..8139)
                     /locus_tag="PK513_06915"
     CDS             complement(6766..8139)
                     /locus_tag="PK513_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOO82216.1"
                     /translation="MEEDWFKIEYLIGPPVFLLLIFIALILHELGHYWAARLFKMPVD
                     SVVIGRGRTLRHWGDHHGTRWSFRRWPLGAHVHLQGLEGLSEGGSFAARPFWQRTVTI
                     LAGPLANLAILPFLFFAFYILVGQPSTPPVLVGIEPGLAADKAGMQVGDRFLAVDGKP
                     VANFQDIWRVAYSKGAVQSIYTIQRGDKIFDIPFTPGWTEYKDEDGIERKNARFGVTW
                     QHSPLKLSAVMSVKGQNTEDDEDKARRLLLKNMGKEIIVGFEGPDGKEKPARVILSTA
                     QNVNLDNPDHDDYERVFFGQTRGNIYLDRPAFEYAIEALRKSATMIKNVAGTPFQLLP
                     IDPYKLKDSSEVMHEDTRALNVFYKYMHLFALTCVIIALVNLLPLPYMDGGHFLTQTI
                     ERVLKKPLSRKRKATLFVLIFFSLYAIVVVSNMDKVPGYIDSRLEKVHDFIDKSQPKY
                     KDEASNG"
     gene            complement(8173..9234)
                     /gene="gcvT"
                     /locus_tag="PK513_06920"
     CDS             complement(8173..9234)
                     /gene="gcvT"
                     /locus_tag="PK513_06920"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOO82217.1"
                     /translation="MGAKMGEFAGYDMPLYYGEGVIKEHEWVRESCGLFDVSHMGQAM
                     VQGADALEFVQKLTPSSFEKLGNMRTKYTVLLNEEGGIIDDLMITKTGENAFHFVINA
                     GCKDKDIAWMKQHLTPNLEFTHFDDWALLALQGPESESVICKILGTDLSDLPYMGLWA
                     KDDHKMFISRLGYTGEDGFEIAVPNADAAALAEKFLAHDAVKPIGLAARDSLRMEMGY
                     ALYGHEITAETSPVEAGLSWVIGKNASGYFGYDRIQKELAQGTARKLVGIKLTDKGVA
                     REGAEIRDEADEVIGVLTSGGHSPTLKESIGMGYINSDLANVGQKIFINVRGRNIAAV
                     VAKLPFVPAKTKSMKKAAA"
BASE COUNT         2053 a         2353 c         2407 g         2424 t
ORIGIN      
        1 ttcttgattt cttcggcttc gtcttttttc gcaccttctt tgatgttacc accagcttca
       61 acaaggtctt tagcttcttt caagccaagg cctgtgattg cgcggacttc tttgatcaca
      121 gcgattttgt taccaccagc cgattcaaga acaacatcaa atgagtcttt ttcttctgcg
      181 gcgccagcgg catcaccgcc agcaacggca gcgacagcaa cgggcgctgc agcagcggct
      241 gttacacccc acttgtcttc aagcaatttt gagagttcag cagcttccaa aacagacagt
      301 tctgagagtt gttcaacaat tttttccaga ttagcagcca taatttttct ccttatatat
      361 agctaaaatt taaaattcct actcgccttt ttctccatag gctttcgtaa caccaacaag
      421 ctcgcgtgcc ggcgcctgca tcagtccagc cagtttcgtg gccggggcct gcaacagacc
      481 aacaagcttg gagcgaactt catcaaggct tggcaatttc gccaacgcct caatccctgc
      541 ggcatccagg accagctcac ccagagcccc tccgaggatg atcaatttat cattgccctt
      601 ggcaaattta taagcggctt tcgcggcagc tacaaagtcc tgagacgtcg caacgccggt
      661 cggtcctgtg aacatgtcgc tgatcgcttc aaattttgtg cctttaagcg caatttttgc
      721 caatgtgttc ttggtgactt taaacgaagc accttcgctg cgcagaccgg cgcgcaggtc
      781 agccatttct ttaacagtca ggccggaata atgtgtcaca acaaccaatt catcattggc
      841 gaaacgctca ttcagagact gtatttctgt ttttttctgt gcgcgactca tgcccatcgc
      901 ttttctcctt aaattccaga tccccgctgt ttaagctcac tttgttcgcc gcagggataa
      961 cggcgtgtga gaataaaaaa gtggcccctt tgatttccaa agagccaccc atccgaataa
     1021 atgaaataat aaatctgcaa cgcagaccag tgtttttcac tctatctatg caggacttat
     1081 agatgcctac aatctcggac aggttgtata tcggggccga caattaaagc tttatcatcg
     1141 gcccgtcaag atatttttta tgccgctgcg gacaagctgg ccgtgtcgat ctttaggccc
     1201 ggacccattg tggaagtcaa agagactttc ttcacgtagg tgccttttgc gcctgatggc
     1261 ttggctttga taattgcggc gtagaaagca cggatatttt cagccagctt cttgtcatcg
     1321 aatgaagcct taccaacgcc cgcatgaacc acgcctgctt tttcagcgcg gaattcaatc
     1381 gcgccgcctt tagcatcttt cacggccttt tcaacatcag gtgtcacggt gccaagtttc
     1441 gggttcggca tcaggccttt cggacccaaa actttaccaa gctgaccgac aatcaccatc
     1501 atgtccggcg tggcgatgca gcgatcgaag tccatttcac cggctttgat tttctcagcc
     1561 agatcttcag cgccgacgat atccgcaccg gcagctttcg cggccttggc tttgtcgtct
     1621 ttagcaaaaa ccgcaacgcg cacggacgag cctgtgccgt tgggcaggga aatcatgccg
     1681 cgcacagcct gatcggcgtg gcgtggatca acgttgaggt tgatggcgat ttcgaatgtt
     1741 tcatcgaagt tgcgagcttt ggccgcactc ttcaatgctt tgacagcatc ttcgagtgta
     1801 tattccttgt tacggtctac accttccaaa gccgctttca tttttttcgt ttgtttaggc
     1861 attgtctagt cctccaccac gtcgatgccc atagagcggg cgctgccagc gataattttg
     1921 atggcggcgt caatgtcatt ggcattgaga tcttccatct ttttctcagc gatttctttc
     1981 aattgtgacg tgcggattgt tccgataatg tcacgccccg gtgcaccaga tccttttttc
     2041 tgcttggtgg cctgcttgat gaaatagctg gctggcggtg ttttggtgat gaaatcaaaa
     2101 gagcgatctt caaaaaccgt gatcaccaca ggtatcggaa tgccttttga gtcttctgtc
     2161 ttggcgttaa aggccttgca gaattccatg atattgacgc cgtgctgacc caaagcgggc
     2221 cccaccggcg gggatgggtt ggctgcgcca cccgtaattg tcaggttgat gtaacctgag
     2281 actttctttg ccattgtatt tctccttatt aacaaagagt tttgtggtac ggctcatcca
     2341 tggacttgac ggatgaaagc ctcccacata tttagtgctg cgctttatag cccagcatca
     2401 ctttataaat caagttaaac tttctcaacc tgcccgtatt cgagttccac cggcgtggca
     2461 cggccaaaga tagaaacaga gactttgagt ttttgttttt cttcgtcgat ttcctcgaca
     2521 atgccgctga aggacgcgaa cggaccgtca gtgaccttga tttcctcacc aatatcgtag
     2581 acgacggatg gacgcggacg gtcaatgcct tcctgaacct gctggatcaa gcgattggct
     2641 tcggcctcgg tgatcggcac tggcttattg ccccccgctc ctaaaaagcc actgactttc
     2701 ggtgtatctt tgatcaaatg ccagacattg tcgttcatat cgagtttggc cagcacatag
     2761 ccggggaaga atttgcgctc ggcatttaca cgctggccgc gtttgacttc cacgacttct
     2821 tcggtcggga ccatgatttc ttcaacatgg ctttcgaggc cctgcttgcg ggctttttcc
     2881 ttgatggctt cggcgacttt ttgttcaaag ccggaataaa cgtgaagaac gtaccatttt
     2941 gctgcagaca ttgtttttct ctctttttaa aaattattat agaccaaaac ccatgatcaa
     3001 acgcacaatc catgcaatga tctgatctga aaaatacaga aacaccgagg caataaagac
     3061 catgatgaac acagcgattg tgctgacggt tgtttcatgg cgtgaaggcc aagtgacctt
     3121 tttggcttcg gagcggacct gtttaaaaaa ttcgacgggg ctggccatgt tctaaaaatc
     3181 ctttttgttt gctccgagac acaattcgta gagcgctttg gcgcttttgt tgatctccac
     3241 cgtgtcaggg aatgagactg tttaacgtat acggattgaa aaagaaagca aaaagtgcta
     3301 gattgtgctc atgttatctg caatttttaa attatccggt ctgaatgaag ttcttgaagc
     3361 cagtcaagag gctgttttgc cttacctgac gcgctcgcat gatataactg gtgtggacgg
     3421 aaaaaaagac agctccggca gcggccccac ccttgacatt ggcgctggca gtggcgacta
     3481 atttttccat cttaaaaccg gattgcgggc agccttaact tcgtcgaggc ggcggcgcgg
     3541 tgtggaatgc gggaaactat ggaagtggtc ttcggcatca ctgcgttgct ccgcctcgcc
     3601 agcgatatcc ttcatcgcgg ttataaagcg gtcgaggctg tccttgcttt ccgtatcggt
     3661 cggttcaatc agcatcgtgc cggggacgac gagcgggaaa tacacggtca tcgggtggaa
     3721 acctttttcg atcagcgcct tggcgatatc gagggtctgg acgccacatt ctttttgctt
     3781 tttatcggtc aggagcgctt cgtgcatgca tgggccttgg gcagaaaagg gcgcatggta
     3841 gtcgccttcg aggctgcgca ggatataatt ggcattcaat accgcgtctt cggagacctg
     3901 ttttagcccg tcggcgccgt gggaaagcat ataggccagc gcgcggacat gcatgccgaa
     3961 ctggccatgg aagcctttga gtcggccaca gtttgtcagg cggtcgattt cggtttccaa
     4021 tcgataggtt tcaccctctt tcaacacagt gggtacgggc atgtaagcgc caagttcttc
     4081 ggtgcagcag atcgggccgg agccgggtcc gccgccgcca tggggcgttg aaaaggtttt
     4141 gtgcaaattg atgtgcatga catcgacacc gaaatcgccg gggcggattt taccgaccag
     4201 cgcattaaag tttgcgccgt ccatatagaa gtacccgccg gccttatgca gaagattggc
     4261 aatttcgaga atatcttttt cgaacaggcc gcaagtgttg ggattggtga ccatcatccc
     4321 ggcaatatca ccgccagccg gatcggtttt atagagcgct tttttaaagg cttctacggt
     4381 aagcatcccg gtgtctttgg ttgggatgtt gcggacctca tagccgcagg tggtggcggt
     4441 ggccggattg gtgccgtggg cggaatcggg ggtcagtaca atcttgcgct ttttcccgtc
     4501 gcctttttgt tcgtgggcgc ggcggatggt catcatcccg gccagctcgc catgcgctcc
     4561 agcggcgggc gacaggcaca cgccggacag attggtgagc tcggccagcc attgttgaag
     4621 ttcatacatc agggcgagcg cgccttgggt ggtggattcg ggctgcagcg gatgcaaatg
     4681 cgcaaaaccg ggcaggcgcg cgagtttttc attcaggcgc gggttgtgtt tcatcgtgca
     4741 ggagccgagc gggaagaagc cgtgatcgat ggaatagttc catgtgctca tgcggacaaa
     4801 gtgacgcagg gcctgaggtt cgctgacctg tggcaggccg atattctcgc gcgcctgttg
     4861 gccggtgcgt aatggcatgc ccgcgggggc tggcaaatcc acgccggggt gatcggaacg
     4921 ttccttttcc cagagcaggt tttcttcgta atgcaggccg cggttatcgt cgttcatttt
     4981 tgtgtccata gtttctacaa ttttgttttc cgcgctcatg ctaatgcctc tttcatagcc
     5041 gtggtcaggg cagcaatgtc ttcatccgtg gtcatttcgg tggcggcgat gaggagctga
     5101 ttttcatcga gatcgtaccc agcaatgatg ccctgcccgg caagagcttt gacgatgcct
     5161 tttgaggccc gcggcaattc cagtgtgaat tcgttgaaga agttctcggt gaggagcttc
     5221 acaccaggga tttgagtcag cgcgtcggcg gtacggcagg ccgcttcgtg gttgaggcgc
     5281 gcaagacgtt taaagccatc ttcacccagc aggctcatat gcacggtaaa ggcgagcgcg
     5341 cacaggccct ggttggtgca gatgttagag gtggccttgt cgcggcggat atgctgctcg
     5401 cgggtggaaa gcgtgaggac aaagccgcgc tttccatcgg catcttcggt catgccgcac
     5461 aggcgtccgg gcatctggcg caagtatttt tccttacagg cgaaaaaccc aagatatggc
     5521 ccgccatagc tgagcggaat accgatggat tgggcttcgc cgcaaacgat gtcggcttcg
     5581 gcgggagcag gcagcaggcc aagggagacg atttctgtga cgacgacgat cagcagagca
     5641 ccggtttcat cgcaggattt gcgcagggca tcatatttat gcggcgcgcc gtagaaatcg
     5701 ggtgactgaa taatgacgca ggcagtctgc tcatcgggcg cgccgtcggt gattttaaca
     5761 tcgccatcga gattccacat gtaggatttg agcacaccgc gataatcagg atgcagagcg
     5821 tttccaataa cgatcttttt gcgcttggtc acgcgcaagg ccataagtgc ggcttcggta
     5881 gtggcggtcg caccgtcata catgctggcg ttggcgacgt cctggccggt aagctgggcg
     5941 atgaaggtct ggaattcgaa aatcgcctgc aatgtgcctt gggcgatttc gggttggtag
     6001 ggcgtgtagg cggtgaggaa ttcgctgcgc tggatgatgt aatcaacgct agccggaatg
     6061 tggtggtaat acgccccggc gccgaggaaa aacggcccgg cgctggcggc gtgattttgg
     6121 tttgcatagg cgtttaaatg acgctcaacg tcaagctcgc cttgatgatc aggcaggttg
     6181 aatttgtggc ccatctcagt agccggaacg tcaacataaa gatcatcaat actgcccacg
     6241 ccgatggttt taagcatcgc cgcgcgcgca ttttgggtct gggggaggta acgcatgttt
     6301 tctttctctc tgtcattgcg aggagcgcag tgacgaagca atctataaat cggaaaactg
     6361 gattgcttcg ctgcgctcgc aatgacgtct tcattttaat ccaaagtctt caaataatcc
     6421 ttatacgtgg cctcatccat caggtcgccc agctgcgccg gatcggtgat tttgattttg
     6481 gcgatccagc cgccgtcggc cagcggcttg gcgatcagtt ctagatcatc tggcatattg
     6541 tcgttggcct caacgacttc gccatcgatg ggggaataca cttcggcagc ggttttgacc
     6601 gattcgacga cggcgaattc atcacccttg ctcacctgtt tgcctatttc aggcaattcg
     6661 acaaagacca gatcgcccag cgcttcgcgc gcatattctg tgatacccac ccatgcgaga
     6721 tcaccatcaa catatatcgc ttcgtgatcg gtggtaaatt ttaactcagc cattgcttgc
     6781 ctcgtcctta tattttggtt gtgatttatc tataaagtcg tggacttttt ccagacgcga
     6841 gtcaatgtag cccggtactt tatccatatt agagacgaca actatggcat agagactgaa
     6901 aaatatcagc acaaagagcg ttgcttttct tttgcgactc aacggctttt taagaacgcg
     6961 ttcgatggtt tgcgtcaaaa agtggccgcc atccatatag ggaagcggca aaaggtttac
     7021 caaagcaatt atgacgcagg tcaatgcaaa gaggtgcatg tatttgtaaa atacattgag
     7081 ggcgcgtgta tcttcgtgca tcacttcact ggaatctttg agcttgtaag gatcgatagg
     7141 caataattga aacggtgttc ctgcaacatt cttgatcatt gtcgcgcttt tgcgcagtgc
     7201 ttctatggca tattcaaagg ccgggcgatc aagatagata ttgccgcgcg tttggccaaa
     7261 aaacacgcgt tcatagtcgt catgatcggg gttatcgaga ttgacgttct gcgcggtaga
     7321 gagtattacg cgggccggtt tttcttttcc atcagggcct tcaaagccga cgattatttc
     7381 cttgcccatg tttttgagca gtaacctgcg cgctttgtct tcatcgtctt cggtgttttg
     7441 ccctttgacg ctcatcaccg cactgagctt aaggggagaa tgttgccagg tgacaccgaa
     7501 gcgggcattt ttgcgctcaa tgccatcttc gtctttatat tccgtccagc ccggcgtgaa
     7561 aggtatatca aaaattttat cgccgcgctg gatcgtatag atgctttgaa ccgcgccttt
     7621 ggaataggca acgcgccaga tgtcctgaaa atttgcgacg ggtttcccat caacggcgag
     7681 gaaacgatcg ccgacctgca tgccggcttt atcagcagcc agccccggtt caataccgac
     7741 caaaaccggc ggcgttgagg gttgaccgac caatatatag aaagcgaaaa acaggaatgg
     7801 caggattgca agatttgcca aggggccggc gaggatggtt acggtgcgtt gccaaaaagg
     7861 ccgagcggcg aaagaaccgc cctcggacag gccctccaac ccttgaagat gcacatgagc
     7921 gccgagcggc caacggcgaa acgaccagcg tgtaccgtgg tgatcacccc aatgtctgag
     7981 ggtacgaccg cggccaatga caacggaatc gacaggcatt ttgaacaggc gcgcggccca
     8041 gtaatgaccc agttcatgta agatcaatgc gataaaaatc agcagcaaaa aaaccggcgg
     8101 gccgatcaga tattcgattt taaaccagtc ttcttccatt ttattgtgtt tactttcctg
     8161 atgctgggcc ctttaggctg cagccttttt cattgatttg gttttggccg gaacgaaggg
     8221 cagtttggcc acgacagccg caatatttcg cccacgtaca ttgatgaata ttttttgacc
     8281 aacattggcc aagtcactgt ttatataccc cattccgatt gattctttga gtgtggggga
     8341 atgaccgccg gaagttagaa caccgataac ttcatcggct tcatcccgga tttcagcccc
     8401 ttcacgggca acgcctttat cggtgagttt gatcccgacg agcttgcggg cagtgccctg
     8461 agccaattct ttttggatgc gatcatagcc aaaatagccg ctggcgtttt tgcctatcac
     8521 ccaactcaat cctgcctcaa caggcgaggt ttcggcggtt atttcatgac cataaagcgc
     8581 atagcccatt tccatacgca gcgaatcgcg cgcggctagg ccgatgggtt tgaccgcatc
     8641 gtgggcgagg aatttttctg ccagcgctgc ggcgtccgcg ttcggcacgg ctatctcaaa
     8701 accatcttcg cccgtgtaac cgagacggct aataaacatc ttatgatcgt ctttagccca
     8761 tagccccata tagggtaaat ctgacagatc cgtgcccaga atcttgcaga taacgctttc
     8821 cgattcgggg ccttgcaggg ccaaaagagc ccagtcatcg aagtgggtaa attcgagatt
     8881 cggggttaaa tgctgtttca tccaggcgat gtctttgtct ttgcatccgg cattgatgac
     8941 aaaatggaaa gcattctcgc cggtttttgt gatcatcaga tcgtcgataa tgccgccctc
     9001 ctcgtttagc agcacggtgt atttggtacg catattccca agcttttcaa aagatgaggg
     9061 ggttagtttt tggacaaact ccagtgcgtc tgctccttgc accattgcct ggcccatgtg
     9121 ggaaacgtcg aacaaaccgc aggattcacg cacccactca tgctctttga ttacgccttc
     9181 gccgtaataa agcggcatat cataacctgc aaactccccc attttagcgc ccagctt
//
LOCUS       DAOSWR010000081         9121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4848_length_9121_cov_5.036731, whole genome shotgun sequence.
ACCESSION   DAOSWR010000081 DAOSWR010000000
VERSION     DAOSWR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4848_length_9121_cov_5.036731"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..793)
                     /locus_tag="PK513_06925"
     CDS             complement(11..793)
                     /locus_tag="PK513_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO82218.1"
                     /translation="MADYDYDYFVIGAGSGGVRSARIAAGHGAKVGVAEKSALGGTCV
                     NLGCIPKKLFAYASDYTGHFEDARGFGWDAPAESSFDWRALINNKNNEISRLNGIYKN
                     LLENSGVEILSGRARFIDDHTLEVGKRQVSADKILIAVGGRPRLPDIEGGELMATSDD
                     VFFWPKMPEKVVIQGGGYIAVEFAHILHGIGAHVTLLYRGELFLRGFDRDIARSLADE
                     MAKQGVDLRFNCDITRVERKGENGFIVYTEVVSVLRPALRRS"
     gene            complement(796..1680)
                     /locus_tag="PK513_06930"
     CDS             complement(796..1680)
                     /locus_tag="PK513_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO82219.1"
                     /translation="MTDDFLKQLRYAGEAFGLAVLFLVFLVLPAETASNLGGWIGRSI
                     GPKLAASRKVRHNLNRVFSDITETCSDEIIAGMWEHLGRVIAEYPHLETLALGHTQII
                     GQDNLQNALNNPNGAIFFGAHLGNWEINGAAVLLQAGRKVALSYRAPNNPWSDKLLMR
                     ARTLGGRLPAYSKSRQGGKAMIETIKNGRTLGILIDQKYNQGVSVPFFGLEAMTNPFF
                     VQLAQKYGCALVPIRNRRLDGASFSLSVYEPLTVFEDDGKPRAIEDVIRDAHTVLEEF
                     ILETPEQWLWLHRRWKDS"
     gene            complement(1677..2684)
                     /gene="lpxK"
                     /locus_tag="PK513_06935"
     CDS             complement(1677..2684)
                     /gene="lpxK"
                     /locus_tag="PK513_06935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOO82220.1"
                     /translation="MTLKTPSFWYRPAGSKPPAVERLLIPAAAVYRFLYDIHHIMKRP
                     YKARVPVLCIGNLVAGGTGKTPTAIALLKTLKAHGIAKNPAFLIRGYGGAEIGPMRVD
                     PKIHTAWDVGDEALILAQHAPTYVGGDRAASAQMALENDADLIIMDDGLQNPSIHKDL
                     RLVVVNGEMGFGNKRLLPAGPMRQPLDENYSMADGVILIGEDRRNALQGLPDSIPLIQ
                     AQLKPSASAQIDTQTKYLAFAGLGYPQKFFCYLKGTLGMNVVSESAFSDHHPYEISDL
                     RALHKQAESLGARLITTEKDYLRLPHIEGVAVETLPVEMCWDNEKALLQLIQKHLKPQ
                     L"
     gene            complement(2681..3976)
                     /locus_tag="PK513_06940"
     CDS             complement(2681..3976)
                     /locus_tag="PK513_06940"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOO82221.1"
                     /translation="MVLSLYSFSMRAAKPLITAYLKRRQRAGKESSSRFDERLGVVQC
                     PRPKGRLIWVHAASVGEVQSALILIHKLLALYEGVQVLVTTGTRSSAKMMETALPEGA
                     FHQYYPVDNPEWVEAFLNHWSPDLILWMESELWPNMLLAIQARKIPAILINARLSPRS
                     FRIWKLLKGASRKILLSFTLILCQTDKDESFYRRLGAQNVQVSDNLKYSAAPLPCDEA
                     ALKAINSAFINRPCWVYASSHASEEKIAAQIHARLKNSIPNLLSIIIPRHPERRMAIA
                     QDLDGLGLNIVFRGGDKTLPGDDTDIYIADTMGELGLFYRACPVAVIGRSFSDDGGGG
                     HNPIEAAQLHCAVLHGPNVQNLQEIFDEMDAYGAAMRLSTPEELESHLYDLLTNAEKI
                     EVLQQKAYDFSRRKEQVLETVLEALRPTLDTLEIGRKAA"
     gene            4113..4400
                     /locus_tag="PK513_06945"
     CDS             4113..4400
                     /locus_tag="PK513_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82222.1"
                     /translation="MKRIYRKRKSKKLREDTLQQLQKTRMHFEEEYPELLEKARKALK
                     QTGQTPKSEGVNIADETVDRKKTLEVVLKYAATRTGKQKNNLKQELSKFLN"
     gene            complement(4428..5294)
                     /locus_tag="PK513_06950"
     CDS             complement(4428..5294)
                     /locus_tag="PK513_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82223.1"
                     /translation="MKLDIKLLKVLFAAALAGFISVSAAQAAGLFAPDPCDPEYYESL
                     KSRAWLEAQREITQNQNLIFKADSVLEYTCFDGYLKELADHAGPPVAAGKMFTGTNRW
                     GGVPGNMSTTLSRLVAAPMDAYDTANFNHTLLGGRTGSGVNLPASISPGNYTCNVMDQ
                     VWKQAKCMDFIHTAAEDGFFTFDQYHAQADKRFLPTRCPKTANFKAEQDKAIETATTP
                     WTEDDVITYFGLIFPSGGCGSANGQFASKIKTGLVIERTSGGVSKYNEYVCVVPGCYY
                     EPTGQNSGSCKN"
     gene            complement(5397..6365)
                     /locus_tag="PK513_06955"
     CDS             complement(5397..6365)
                     /locus_tag="PK513_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82224.1"
                     /translation="MPCETKPSAAQLLNPETGPNSSKDWPAVRSWPAGPTRPNACDAN
                     FMNQIYAKAFIEAEREMVITNATITKPDSVLEYTCFDYLAARVATIAGPIFSESTRWH
                     PVTVNIMGESVTIDVFLGNTHLDRSIENLVLQGLDRYGEKSFPYDFLAGAAVGDNHDF
                     SPTVAGASGVCDHMYNMHYISKCEDFGLNVPFMTFESYFSTPALLNTDPRTKPKACPG
                     THQITPMRVEIAKNKDWSWAAFDKVDTLLPILKAPASGSGCANATPIPTGVMVKKSTY
                     AQDLAGNPIMGAAQFEYEEKVCTNPACYFDNKDNANPADDVCKAAP"
     gene            complement(6484..6888)
                     /locus_tag="PK513_06960"
     CDS             complement(6484..6888)
                     /locus_tag="PK513_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82225.1"
                     /translation="MLGISYAQAAESLRFDTPLAYSSKEKIKSFTVNELSSPLEKFDL
                     AGTDLNGDGLHEFIVRTKMCERAPLCDYFILGEAKNGMINLGKIEAAYILLGNDYAHG
                     VRNLLVFDNQENDFDFQLYTWDPEQSLYREAR"
     gene            complement(7032..7253)
                     /locus_tag="PK513_06965"
     CDS             complement(7032..7253)
                     /locus_tag="PK513_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HOO82226.1"
                     /translation="MVEIHPEEVSRLQSYLQGKLKNTGFALKIREKARDSVEVLLNGE
                     FIGVIYKDEDGDFNFDMPILEIDLPGATS"
     gene            complement(7269..7883)
                     /locus_tag="PK513_06970"
     CDS             complement(7269..7883)
                     /locus_tag="PK513_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO82227.1"
                     /translation="MQGKPYTDSQVIYVSNHISYLDIPALASVICYGSFVAKKDVASW
                     PVFGYLSKLQQTAFVSRSREDARKGANALAEMLKDGKNLIIFPEGTSTDGREVVPFKS
                     SLFSIMLQKDAQNLVIQPVSIEVVSSNGRAPKTQEERDLYAWHRDMDTELPEHLWRFA
                     KTSGAVLNIKFYDIVRANDFSDRKTLAKVCHETVSKGLKLSKAA"
     gene            complement(8048..8899)
                     /locus_tag="PK513_06975"
     CDS             complement(8048..8899)
                     /locus_tag="PK513_06975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOO82228.1"
                     /translation="MSHIELELESISVRLAETQAEMEAAQALRYTVFYEEYDAIPTAE
                     MVADRRDFDEYDAYADHLIVIDSSGASDKIVGTYRMLQRSGADQVGQFYSSSEYDLSP
                     LLKCGSTLLELGRSCVLPDYRTRPILQLLWQGIADYISEYDIELMFGCASLHSTDIKS
                     ISKPLSYLHHYHLAPETIRPRALKGQYINMDIIPQEDINARRVFAELPPLIKGYLRAG
                     SCIGDGAVIDEQFNTTDVFVVVQTHLLADRYRKHYERKIQKTIPGSDDAENNVHELSA
                     FKKTKES"
BASE COUNT         2490 a         2266 c         2056 g         2309 t
ORIGIN      
        1 cgatcgtagc ttaacttcgc cgcaaagctg gccgaagaac cgagaccacc tctgtataaa
       61 caataaagcc gttctctccc ttacgctcga cgcgcgtaat atcgcagtta aatcgcaggt
      121 caacgccttg cttggccatc tcatcagcca ggctgcgcgc aatatcgcga tcaaaaccac
      181 gcaaaaacag ctctccacgg taaagcaggg taacgtgcgc accgatgccg tgcagaatat
      241 gtgcaaattc cactgcaatg taaccaccac cttgaatgac taccttttca ggcattttcg
      301 gccagaagaa aacgtcatcc gatgtcgcca tcaattctcc gccttcaata tcgggaaggc
      361 gggggcggcc gccaacggca atcagaattt tatcggcgct aacctgccgt ttgccaactt
      421 caagtgtgtg gtcatcaata aagcgggcgc gtccgcttaa aatctccaca ccggaatttt
      481 caagtaaatt cttataaatc ccattgagtc tactgatttc attattttta ttattgatca
      541 atgcccgcca atcaaagctg ctttctgccg gtgcgtccca gccaaacccc cgcgcatctt
      601 caaaatgccc cgtataatcg gaggcatagg caaacagctt cttcggaatg cagcccagat
      661 taacgcaagt tccgccaaga gccgatttct ctgcaacccc aacctttgcg ccgtgcccgg
      721 cagcgatgcg tgctgagcgc accccaccgg aacctgcccc aattacaaaa tagtcgtaat
      781 cataatcagc catttttatg aatccttcca ccgcctatgt agccacagcc attgctccgg
      841 cgtttcaaga ataaattcct ctaacacggt atgcgcatct ctgatgacat cctcaatggc
      901 gcgcggcttg ccatcatctt caaacaccgt caaaggttca taaacactaa ggctaaagct
      961 cgcgccgtca aggcggcggt tgcgaatagg cacaagcgca cagccatatt tctgcgcaag
     1021 ctggacaaaa aacggattgg tcatcgcctc gaggccaaag aagggaacgc taacgccctg
     1081 attatatttt tgatcgatca aaatgcctag cgtccggccg tttttgattg tctctatcat
     1141 ggctttccca ccttgacggg atttggaata ggcaggtaaa cgccccccaa gagtgcgcgc
     1201 acgcatcaaa agtttatcgc tccacggatt gtttggtgcg cggtaactta gggccacttt
     1261 tcgtccggct tgcagcagca ctgccgcgcc attaatttcc cagtttccta aatgcgcacc
     1321 aaagaaaatt gctccattgg gattattcaa agcgttttgt aaattgtctt gcccaatgat
     1381 ttgggtatgg cccagagcca gagtttccag atgagggtac tcggcaatca cacgccctaa
     1441 atgctcccac attccggcga tgatctcatc gctgcatgtc tcggtaatat cagaaaaaac
     1501 gcggttcaga ttgtgccgta ctttacggga tgcggctagt ttaggcccaa tgctccggcc
     1561 gatccacccg cccagatttg atgccgtttc ggcgggcaaa actagaaaca ccagaaacaa
     1621 aacggcaagt ccaaaagcct cgcctgcgta acgaagttgt ttcagaaaat catccgtcat
     1681 agttgtggtt tcagatgttt ttgaatcagt tggaggaggg ccttttcatt atcccagcac
     1741 atctcaacag gcaatgtttc aacagcaacg ccttcaatat gcggcagacg cagataatcc
     1801 ttctcggtcg tgatcaggcg tgcacctaag gattctgcct gtttgtgcaa ggctctaagg
     1861 tctgaaattt cataagggtg atggtcgcta aacgcactct cggaaacaac attcatgccc
     1921 agcgtgcctt taagataaca aaagaatttt tgcggatatc ccagcccggc aaaggccaga
     1981 tacttcgttt gtgtatcaat ttgggcagaa gcgcttggtt taagttgcgc ctggattaac
     2041 gggatgctat caggtaagcc ttgcaaagca ttgcggcgat cctcgccgat caatataacg
     2101 ccatccgcca ttgagtaatt ttcatccaga ggctggcgca ttggtccggc aggtagcagg
     2161 cgtttgttac caaagcccat ttcaccattg accaccacca gccgaaggtc tttgtgaata
     2221 ctcggatttt gcaatccgtc atccatgata attaaatctg catcgttttc caacgccatt
     2281 tgtgcgctgg ctgcccggtc gccgccgaca taagtcggtg catgttgtgc aagaatgagc
     2341 gcttcatcgc caacatccca ggccgtatga atttttgggt ccacacgcat cggcccgatt
     2401 tccgcaccgc catacccgcg aatgagaaag gccggatttt tggcaattcc atgggctttc
     2461 agagttttga gcaaggcgat ggctgtgggt gtttttcctg ttccccccgc aacgagattg
     2521 ccgatgcaca agaccggcac acgggcttta tatggccgtt tcataatatg gtgtatatcg
     2581 tacaaaaacc tgtaaacggc cgccgcggga atcaaaagcc gttcgaccgc aggaggcttg
     2641 ctccctgcag ggcgatacca aaagctcggg gttttcagcg tcatgcagcc ttcctgccga
     2701 tttcaagagt atcaagggta gggcgcaaag cctctaaaac agtttcgaga acttgctcct
     2761 tgcgccggga aaaatcatac gctttttgtt gtaggacttc aattttctcc gcgtttgtaa
     2821 gcaaatcata taaatgactt tccagttctt ctggcgtgct taggcgcatc gccgctccat
     2881 atgcatccat ttcatcaaag atttcctgta aattttgcac gttagggccg tgaagcacag
     2941 cgcaatgaag ctgggcggct tcaattgggt tatgtccgcc gccgccatca tcactaaacg
     3001 agcgcccaat aacagccacc gggcaagcgc ggtaaaacag gccaagctcg cccatagtat
     3061 cggcaatata aatatccgta tcatcaccag gcagggtttt atccccgcca cgaaaaacaa
     3121 tattcaaccc cagcccatcc agatcttgcg caatggccat gcgcctttca ggatgacggg
     3181 ggataataat gctaagaagg ttggggatac tgtttttcag gcgggcgtga atttgtgctg
     3241 ctattttctc ttcactggca tgagaactgg cataaaccca gcatggtcgg ttaataaacg
     3301 cgctattgat ggcctttaag gcggcctcat cacagggcag gggagcagcg ctgtatttca
     3361 aattatcact gacctgcacg ttttgcgcgc ctaaacgacg ataaaaagat tcatctttat
     3421 ctgtttggca taaaatcagc gtaaaactga gtaaaatttt acggcttgct ccctttaaaa
     3481 gcttccagat tcgaaaagaa cgcggagaaa gccgtgcatt gatcaaaata gcgggtattt
     3541 tacgggcttg aatagccagc agcatattcg gccatagctc agattccatc cataatatta
     3601 aatctggcga ccaatggttt aaaaaggctt ccacccattc aggattatca accggataat
     3661 attgatgaaa cgcgccttca ggtagcgctg tttccatcat tttcgccgaa cttcgcgttc
     3721 ctgtagtaac aagaacctgc accccctcat agagcgcaag aagcttgtga ataaggataa
     3781 gagcagattg cacctcacca acactggccg catggaccca aatcaagcgc cccttagggc
     3841 gcgggcattg caccactccc aaacgctcat caaagcgcga agagctttcc ttacccgccc
     3901 tttgacgcct tttaagatac gccgtgatca aaggcttcgc cgcgcgcatg ctaaagctgt
     3961 aaagagaaag taccatgaaa acttaactta ttttctgtta tcgtaacact tggttaggat
     4021 ctttggttta gagtgaagat ataagggctg gagccctttt tggcaattcc taactgcatg
     4081 gaaggcttga aaactaaccg ggatgttttt gtatgaaacg catatatcgt aaaagaaaat
     4141 caaagaaact gcgcgaagat acgctgcagc agctgcaaaa aacgcgtatg catttcgaag
     4201 aggaatatcc tgaattgttg gaaaaagcgc gcaaggcact aaagcaaacc ggtcaaacac
     4261 caaaatctga aggtgtaaac attgccgatg aaacggtcga tcgtaaaaag acgctggagg
     4321 ttgttttgaa atatgcggca acccggacgg gtaagcaaaa aaacaacctg aaacaagaac
     4381 tctctaaatt cctgaattag aaattaaccc atcgaagaag ttaatttcta atttttacaa
     4441 ctccccgaat tctgtcctgt aggctcatag taacacccag gcacaacgca gacatattcg
     4501 ttatattttg atacgccacc ggaagtccgt tcgatcacga gacctgtctt gatctttgat
     4561 gcaaactgcc cattggcaga gccacagcct ccggaaggaa aaataagacc gaaataggtg
     4621 ataacatcat cttccgtcca gggtgttgta gcggtttcaa tggccttatc ctgctctgct
     4681 ttaaagttgg cggtcttcgg gcagcgcgtt ggaagaaagc gtttgtctgc ttgggcatga
     4741 tattggtcaa aggtaaaaaa accgtcctca gccgctgtat gaatgaaatc catacattta
     4801 gcttgtttcc aaacctgatc catgacgtta caagtgtaat ttcctggact tatgctggca
     4861 ggtaaattaa cgcccgatcc ggttcgcccg cctaaaagag tatgattgaa attagcggta
     4921 tcatacgcat ccataggggc tgcaacaagt cggctaagtg tagtgctcat attgcctgga
     4981 acaccgcccc aacggtttgt tccggtgaac atcttgcctg cagcgacagg agggccagca
     5041 tgatcagcca attctttcaa ataaccgtca aagcaagtat attccaaaac actgtcagcc
     5101 ttaaaaatca gattttgatt ttgagtaatt tcacgctgcg cctccagcca cgcacgcgat
     5161 ttaagcgatt cataatattc cggatcacat gggtcaggtg caaaaagccc cgccgcctgc
     5221 gcggcagaaa cgctgataaa accagctaaa gccgctgcaa acaatacctt aagcagttta
     5281 atgtctaatt tcatatcgcg tcgaatatac cgtatcattc actctctacc ctatataatt
     5341 gtacgctatt gaagtgtttc ctggcaaata gctttttaaa atgttaccca atctccctaa
     5401 ggagcagcct tacaaacatc gtctgccggg ttcgcattgt ctttattatc aaagtaacac
     5461 gcaggatttg tgcagacttt ttcttcatat tcaaattgag cggcgcccat aatagggttt
     5521 cctgccaaat cctgtgcata cgtagatttc ttaaccatta cgccagtagg aataggggtt
     5581 gcattcgcgc acccgctgcc ggaagccggt gctttaagta taggtaacaa tgtatcaacc
     5641 ttatcaaagg cggcccagct ccagtccttg ttttttgcta tttccacgcg cataggcgta
     5701 atttggtgag tgccggggca tgctttcggt ttggttctgg ggtcagtgtt tagaagcgca
     5761 ggcgtcgaaa aatagctctc aaaagtcata aacggaacat tcaggccaaa atcctcacat
     5821 tttgagatat aatgcatatt gtacatatgg tcacaaaccc cggacgcgcc cgcaactgta
     5881 ggcgagaaat catgattgtc gccaaccgcc gctccggcca aaaaatcata agggaagctc
     5941 ttctcgccat atctatccaa accttgtaag acaaggtttt caatggatct atcgagatgg
     6001 gtgttaccta aaaacacatc aatggtaaca ctctcgccca taatattgac cgtaacaggg
     6061 tgccagcgtg tgctttccga gaaaatcggc ccggcaatcg tggcaactcg cgcggccaaa
     6121 taatcaaaac aggtatattc caggacgctg tcgggtttgg tgatggttgc attggtaata
     6181 accatttcac gctcagcctc gataaaggct tttgcgtaaa tttggttcat aaagttcgca
     6241 tcacaggcat tagggcgagt agggccggct ggccatgaac gtacggcagg ccagtcttta
     6301 ctgctgttcg gcccggtctc cggattaagg agttgcgccg cgctaggctt agtttcgcac
     6361 ggcaagccgg ggaggcaagc ggctgacgcg gagtatgccc acagcgttga aaagaacaaa
     6421 gccgcatatg caaaggcgcc aggttgaaag gtcctaaaat aatattttct aaaagaaatc
     6481 atattaccgg gcctccctgt aaagagactg ttcagggtcc caggtataaa gttggaagtc
     6541 aaaatcattc tcttggttat cgaaaaccag taaattacgt acgccgtgag cgtaatcgtt
     6601 gcccaataaa atatacgctg cttcaatttt tcctaaatta atcatgccgt ttttggcttc
     6661 cccaaggatg aaataatcac acaatggagc gcgttcacac attttcgtac ggacaataaa
     6721 ttcgtgcagg ccatcgccat taagatccgt tcccgcaaga tcgaatttct ccaatggcga
     6781 gcttaactca ttgacagtaa aagattttat cttctctttg ctgctatagg cgaggggggt
     6841 gtcaaaacgc agactctcag cagcttgcgc ataagatatg cccagcatac acaaaagaaa
     6901 cccggtaatt aaaaaggtta tctgaagtct caaagctact gccccaaatt gctcactcct
     6961 ttaattttag caaattggcg ctaaggctgg caacaaacgc ttttaaaaac agctattttg
     7021 cgcaagtttt tttaactggt tgcccccggt aggtcaattt caagaatggg catgtcaaag
     7081 ttaaagtcgc cgtcttcgtc tttgtaaatc accccgatga attcaccgtt aagcaaaact
     7141 tcaacggaat ccctagcttt ctcacgtatt ttaagagcaa aaccggtatt tttgagtttt
     7201 ccctggagat aggattgtag acgagatact tcttcggggt gaatttcgac cattttatac
     7261 gctcctcatt atgcggcttt tgaaagtttc agacccttgg atacggtttc atgacatact
     7321 ttggcaagtg tttttcgatc agaaaagtca tttgcccgaa caatatcgta aaatttgatg
     7381 tttaaaaccg cgccggacgt cttggcaaag cgccaaagat gttcgggaag ctccgtatcc
     7441 atatcgcggt gccaggcgta cagatcacgc tcttcctgtg ttttcggggc gcggccattt
     7501 gaacttacaa cttcaatgct cacgggctga ataacaaggt tttgggcatc tttctgtaac
     7561 atgatcgaaa acaggctgga tttaaatggt acaacttccc gcccgtcggt agacgtacct
     7621 tcagggaaaa ttatcagatt tttaccgtct ttaagcatct cggcaagcgc attggcaccc
     7681 ttgcgggcat cctcacgcga gcggctcaca aaagcagttt gttgaagctt tgataaataa
     7741 ccaaacaccg gccaactcgc gacatccttt ttagccacga atgagccata gcaaatcacc
     7801 gaagccagcg ctggaatgtc gagataagag atatgattgc tgacataaat gacctgagaa
     7861 tctgtataag gtttaccttg catttgaacg cgaatgccaa agacagcgca tacagctttg
     7921 tgccaaagat gcggaagtat ataggcccct cgtcctttgt aaactagcag taaaagtgct
     7981 tgaagcggca caactagagc gcagagcaga gcaaaaccaa aaagctttag ggacgcgaat
     8041 gtgaggttca tgattctttc gtctttttaa aagcggaaag ttcatgcaca ttattttcag
     8101 cgtcatcact gccgggaata gttttttgaa ttttacgttc gtaatgcttg cgatagcgat
     8161 ctgccagcaa atgcgtctgt acgacgacga aaacatcggt ggtgttgaat tgctcgtcga
     8221 tcaccgcacc gtctccgatg catgaccctg cacgcaaata ccccttgata agcgggggta
     8281 attcggcaaa gactctgcgc gcattaatgt cttcctgtgg aataatatcc atattaatat
     8341 actgcccctt cagcgctcgc gggcgaatag tttccggcgc aaggtgatag tgatgtaaat
     8401 atgaaagcgg cttggatatg ctcttaatat ctgtgctgtg caggctcgcg cagccgaaca
     8461 tcagctcaat atcatactcg ctgatataat cggcaattcc ctgccagagc agctgcaaaa
     8521 tagggcgtgt acgataatcc ggtaaaacac atgaacgtcc aagttccagc aaagtcgaac
     8581 cacacttaag caaaggggaa agatcatatt cactggaaga gtaaaattgc ccgacctgat
     8641 ctgcacccga gcgttgcaac atgcggtatg ttccgacgat cttgtcactg gccccggaag
     8701 aatcaataac aatcaaatgg tctgcataag catcatattc atcgaaatct cggcgatcgg
     8761 cgaccatttc tgcagtagga attgcatcat attcttcata aaagactgta tagcgcaagg
     8821 cctgtgcggc ctccatctcg gcttgcgttt cggcaaggcg tacagaaatg gactcaagtt
     8881 caagttcaat atgggacatg ataactctgc atcctaaaaa ttaacgccgc aacatcaagc
     8941 attccttgcc tgccgtaggc ataaatgcag agacaactat tttacaaaca ggtgaacctc
     9001 attggtcacg gcctgcgcac tcttattata ggacaagtca atacgctcat gcggcaggcc
     9061 catttgtgtc aatgttcgca ggactttttc agcattgcgc cgggctttcg tgctctcaat
     9121 g
//
LOCUS       DAOSWR010000082         9103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4861_length_9103_cov_5.429377, whole genome shotgun sequence.
ACCESSION   DAOSWR010000082 DAOSWR010000000
VERSION     DAOSWR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_9103_cov_5.429377"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /gene="bamA"
                     /locus_tag="PK513_06980"
     CDS             complement(<1..1653)
                     /gene="bamA"
                     /locus_tag="PK513_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOO82229.1"
                     /translation="MRLIDRLFIGILVLTVIMPLSAYAQTLIREIHIQGAERIEPETI
                     QSYMDIKVGDPMTREMLDAGLKSLFATGLFADVTLAQKGSALVVDVLENPVINQIAFE
                     GNDKIEDSALLAEIQMRPRQVFTRTRVQSDVSRLYQIYRRSGRFSVRIEPKVIKLDQN
                     RVNLVFEIQEGDVTKVESIRFVGNKRFDDDRLRSEISTKESAWYRFISSDDRYDPDRM
                     AYDQELLRRFYLAHGYADFRILSAVSELSDERDRFFMTFTVEEGKRYRVGKVSINSQL
                     RNFEPAVLRPEINIKTGEWYNADYVQESVDNLTDALGDRQYAFVNVRPDVQRNREENT
                     VDLVFNIDETPRVFIERIEVNGNMRTLDKVVRREFELVEGDPFNKSKLAKSEQNIRNL
                     DFFETAEVTTKPGSALDKTVVEVEVSEKSTGELSIGAGFSTNDGPLADFRIRERNLLG
                     KGQDLSLATVVAGKRTEFDVSFTEPYFLDRDLSAGFSAFHMTRDYQDESSYDQRRTGG
                     GVHMGYPLSERWRQGLRYRMERNEITDVQSDASRFIRGQEGKR"
     gene            complement(1834..3216)
                     /gene="rseP"
                     /locus_tag="PK513_06985"
     CDS             complement(1834..3216)
                     /gene="rseP"
                     /locus_tag="PK513_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOO82230.1"
                     /translation="MNFFEIFQMLGGNVWFYGGAFILVLSILVFVHEWGHYIVARMCG
                     IRVETFSIGFGKELLGFDDKNGTRWKVSMIPLGGYVKLFGDVDPASAGHAEGAEDEQG
                     EIRPFTEEEKTQAFFAQSVWKRAAVVIAGPAINYIFAILIMAALFTFNGQPVTPPVAA
                     AVIGGSSAEKNGFLPHDRIISVDGKSIDTFEDIRREMMIALDLEKHFVVERDGELIDI
                     MASPEKQTQEDRFGFKHSRGLLGLISPRHAVQIKNVKVIDGRSYDDEAAVREALIKRM
                     GKSFKIEIENGQSKQALLVKPLPEFNEFINTAKAEEYGFLFLSNVDANTFIQYPIHKS
                     AFEAIKQCWVITRGTLEALGQMVTGSRSAAELGGVIRIGALAGDMAKQGVIALIMFTA
                     LLSINLGLINLFPIPMLDGGHLMFYAFEAALGRPIPERIQEYAFRIGFAFLIGVMVFA
                     NLNDIMQLLL"
     gene            complement(3218..4405)
                     /gene="dxr"
                     /locus_tag="PK513_06990"
     CDS             complement(3218..4405)
                     /gene="dxr"
                     /locus_tag="PK513_06990"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOO82231.1"
                     /translation="MKKRVSILGVTGSIGQSCADVIASAPQRFEVCTVTANEDAQGLA
                     AAAVRLQARRAIIADPLHEAVLRDALEGQGVAVLSGPEALEQAVQADTDLSIAAISGM
                     AGLRPLMHAIKNSKVVAIANKEPLVAAGALVKAAAEKYNTRILPIDSEHNAVFQVFDP
                     AQRANIQRIILTASGGPFRTWSLEEIEKATPEQAVVHPNWDMGAKISVDSASMMNKAL
                     EVIEAYVLFDMPAEQIDVLVHPQSVVHSMVEYADGSILTQMGASDMRTPIAYALAWPG
                     RMNTPGQRLDLAKMSQLNFETPDLERFPALRLAYECLEKGPMACLTLNAANEVAVAAF
                     LAGKITFPDILRTIEFALGQEPDISLNTIDSVEKADEVVRVRISTYIKDELLPSMMKV
                     KAV"
     gene            complement(4415..5101)
                     /locus_tag="PK513_06995"
     CDS             complement(4415..5101)
                     /locus_tag="PK513_06995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOO82232.1"
                     /translation="MGRFNLKALQERAISAAVLIPVVLFAVIWGGIPFYIMIALLFVI
                     SAYEWVSMVRKIDDTALHVLWGLVYLAVGFLCCIVIREEQSLNAAILFVVMVWSADIG
                     AYFAGKYFGGAKLAPLISPNKTWAGLGGACDSAGLAAVLYYLGVIAFSFEAVFSWVAL
                     IFMFFVGVMIAIVAQGGDLLISFMKRKAGVKDTGALIPGHGGLLDRIDSLLLAAPVFF
                     VLTSFFSFNL"
     gene            complement(5107..5835)
                     /locus_tag="PK513_07000"
     CDS             complement(5107..5835)
                     /locus_tag="PK513_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOO82233.1"
                     /translation="MPQDTEIKIPKHVAIIMDGNGRWARARGLPRSAGHKQGAESARR
                     VVKAAMDLGIKYLTLFGFSSENWSRPDGEIKDLMNLLRYYLRSEMAELHKNGAQLRVI
                     GDRHALDDDIVRMIEHAEKLTEDNSAITVVMALNYGGRNDILHAAQRLHNAHLGQKLS
                     FETVEAQFPGFLMTEGIPDPDLLIRTSGEQRISNFLLWQSAYSELVFTDTLWPDFDKG
                     TLEDALAVYARRDRRFGAVTQIEV"
     gene            complement(5852..6394)
                     /gene="frr"
                     /locus_tag="PK513_07005"
     CDS             complement(5852..6394)
                     /gene="frr"
                     /locus_tag="PK513_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOO82234.1"
                     /translation="MSYNKNEIKRRMDGAIDNLAKEFGGLRTGRASVNLLDPVVVEVY
                     GSKMPLNQLGTVSVPEPRMLSVQVWDGGNVKAVEKAIRDGGLGLNPMPEGNNIRIPIP
                     DLNEERRSELTKIAGKYAENTRISVRNVRKDGMDAVKKDDLPEDEEKRLQDEVQKLTD
                     EAIKKIDTMLADKEKDIMTV"
     gene            complement(6417..7154)
                     /gene="pyrH"
                     /locus_tag="PK513_07010"
     CDS             complement(6417..7154)
                     /gene="pyrH"
                     /locus_tag="PK513_07010"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO82235.1"
                     /translation="MNAQTAKDNMKDYKRIMLKMSGEVLMGEGEYGLDSETVKRVAED
                     IKEVVDKSIEVCVVVGAGNIFRGVAGAAEGMDRTTADYMGMLGIVINALALQNALENL
                     GVQTRVQSAIRMDSICEPYIRRRAVRHMEKGRVVIFAAGTGNPYFTTDTAGALRATEM
                     NCDAIFKGTKVDGVYSADPEKDPTAEHYAHINFMEVLTKDLKVMDATAISLARENNIP
                     IIVFSVKEKGNFAKAVASEGTFTIVTN"
     gene            complement(7252..8169)
                     /gene="tsf"
                     /locus_tag="PK513_07015"
     CDS             complement(7252..8169)
                     /gene="tsf"
                     /locus_tag="PK513_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOO82236.1"
                     /translation="MSEITAAKVKELREKTGAGMMDAKKALTEAGGDMEAAMEELRKK
                     GMAKADKKSSRTAAEGLVAVVTDGTKGAVIELNAETDFVSRNEEFQAFVKKVAEKALD
                     VENVEALAASDFGGKSVKDALTDLIAKIGENMALRRSEKLEVSNGAIVSYVHGALAEG
                     LGKIGVLVALESDASADVLEPLGKQVAMHVAAAFPKYLDRNSVDTAEMEKERAFLVEQ
                     AMAEGKPQEIAEKMVEGRMRKYFEEICLLDQKSVIDGETKIADVIANAAKEAGSKITL
                     TAFSRFQLGEGIEKEEEDFAAEVAKAANG"
     gene            complement(8222..>9103)
                     /gene="rpsB"
                     /locus_tag="PK513_07020"
     CDS             complement(8222..>9103)
                     /gene="rpsB"
                     /locus_tag="PK513_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO82237.1"
                     /translation="HFGHHTRRWNPKMRQYIFGVRNGVHIIDLQQTTPLLNEALKAVR
                     DIVANGGRVLFVGTKRQARDKIAESAKRCGQFYVNHRWLGGMMTNWQTITGSINRLRE
                     VQDILGSNEQRAGRTKKEILMLTRERDKLELSLGGIKDMGGQPDALFILDVTMEDIAV
                     KEANKLGIPVFGVVDTNADPAGVDYIIPGNDDALRAIDLYCDLIADAVLDGLQAELAS
                     GGKDPGAAEDVQVKLPKKVKDKEPEALTPGEQAKLDAEKKKKPALKKDSKAEVVKADE
                     PANEAEAEEKAKKQASA"
BASE COUNT         2599 a         2658 c         1638 g         2208 t
ORIGIN      
        1 ccgcttccct tcctgaccac gaataaagcg agacgcatca ctttgcacat cggtaatttc
       61 gttacgttcc atacgataac gcaaaccctg acgccaacgc tcagaaagcg gatagcccat
      121 atgcaccccg ccaccggtgc ggcgctgatc atacgaactt tcatcttggt aatcccgtgt
      181 catatgaaag gcactaaagc ccgcagacag atcccgatca agaaaatacg gctccgtaaa
      241 actgacatca aattcagtgc gttttcctgc gacaacagtt gccagtgaca aatcctgtcc
      301 cttgccaagc aagttgcgct cgcgaatacg gaaatccgcc aaaggcccgt cattcgtcga
      361 aaacccggcc ccgatagaaa gttccccagt tgatttttca cttacctcaa cctcaacgac
      421 agttttatcg agcgcagaac caggctttgt agtaacctca gccgtttcaa agaaatccag
      481 attacggata ttctgctcag attttgccaa ttttgatttg ttgaacggat ctccctcaac
      541 cagttcaaat tcacgacgta caaccttatc gagcgtacgc atattaccgt tcacctcaat
      601 gcgctcaata aaaactcgcg gcgtttcatc aatattgaac accagatcaa cggtattctc
      661 ttcgcggtta cgttgcacat ccggacgtac attcacaaat gcatattggc gatctcccaa
      721 agcatcggtc aaattatcaa cgctttcctg aacataatcg gcattatacc attcaccggt
      781 tttgatattg atttcaggac gcaaaacggc aggctcaaaa ttacgcaatt gagaattaat
      841 agaaacttta cccacgcgat aacgcttccc ttcttcaacc gtaaaggtca taaaaaaacg
      901 gtcccgctca tccgaaagct cggacacggc agaaagaata cgaaagtctg cataaccatg
      961 cgcaagataa aaacgacgaa gcaactcttg atcatatgcc atccggtctg gatcatatcg
     1021 atcatctgag gaaataaagc gataccaggc agattctttc gtgctgattt cactacgcaa
     1081 acgatcatca tcaaaacgct tgttaccaac aaaacgaata gattccacct tggtgacatc
     1141 accttcctgg atttcaaata ccaaattaac acggttttga tccagcttaa ttacttttgg
     1201 ctctatccga acagaaaaac gccctgagcg gcggtaaatt tgataaagac gcgaaacatc
     1261 agattgtaca cgcgtacggg taaaaacctg ccgtggacgc atctgaatct cagccagaag
     1321 cgcagaatcc tcaattttgt cattaccttc aaaagcaatt tgattgataa ccggattttc
     1381 aagcacatcc acaaccaaag cactgccctt ctgggccaat gtcacatcag caaaaagccc
     1441 tgttgcaaac aggctcttaa ggcctgcatc aagcatctca cgcgtcatgg ggtccccgac
     1501 cttaatatcc atataagact ggatagtttc cggctcaatt cgctctgccc cctgaatatg
     1561 aatttcacgt atcaaggtct gcgcatatgc actcaaaggc ataatcacag tcaaaaccaa
     1621 aattccaatg aacaatctgt ctatcagtcg catgggagat caccatcaat aaataataaa
     1681 taaaaaacaa aaacgagaaa gaaatgcctc cccgaaagta cgttcatttt tatcgggtta
     1741 agcgcaaaaa gacaacagcc ttattatgct gattctttta cacaaaaacc agcccttaag
     1801 acaaaactat ccctagaagg ttaagtcatg tacctagagc aaaagctgca taatatcgtt
     1861 aaggttggca aataccatca ctccaatcag aaaagcaaac ccgatgcgaa acgcatattc
     1921 ttggattctt tctggaattg gacgtcccaa ggctgcttca aatgcataaa acattaaatg
     1981 cccaccatca agcatcggaa taggaaacaa attaatcagc cccagattaa tcgacagtaa
     2041 agctgtaaac ataataagtg caataacacc ttgtttggcc atatcgccag ctagggcgcc
     2101 gatacggatt accccgccaa gctctgcggc actgcgacta ccagtaacca tttgtcccaa
     2161 agcctcaagt gtaccgcggg taatcaccca gcattgctta atagcttcaa acgctgattt
     2221 atgaatcgga tactgaataa aagtgtttgc atctacattc gacaaaaaca aaaagccgta
     2281 ttcctcagcc ttagctgtat taataaattc attaaactcc ggcaaaggct taacgagcaa
     2341 cgcctgcttg gactgtccgt tttcaatttc aattttaaag cttttcccca tgcgcttaat
     2401 caaggcctcg cgcaccgcag cttcatcatc ataactacgt ccgtcaataa ctttaacatt
     2461 tttaatctga acagcatgac gcgggctaat taaccccaaa agcccccggc tatgcttaaa
     2521 accaaatcgg tcttcctgcg tctgtttctc cggcgaagcc ataatatcaa tcaactcgcc
     2581 atcgcgctca acgacaaaat gcttttccaa atccaacgca atcatcatct cccggcgaat
     2641 atcttcaaac gtatcaatag actttccatc aacggaaata atcctgtcat gcggtaaaaa
     2701 accgtttttc tctgcgctcg acccaccaat caccgccgcc gccacgggcg gagtcaccgg
     2761 ctgaccatta aatgtaaaca acgcagccat gataagaatc gcaaaaatat aattaatcgc
     2821 cggccctgca atcacaaccg ccgcacgctt ccatacagat tgcgcaaaaa atgcctgcgt
     2881 cttttcttcc tcggtaaaag gacggatttc accctgttca tcctctgcgc cttccgcatg
     2941 cccagcactg gcaggatcaa catcaccaaa cagcttcaca tatccgccaa gcggaatcat
     3001 ggaaaccttc cagcgtgtac cgtttttatc gtcaaagccc aaaagctctt ttccaaagcc
     3061 aattgaaaaa gtctccaccc gaatgccgca catccgcgcg acaatgtaat gaccccattc
     3121 atgaacaaag acaaggatac tcaagacaag gataaaagcc ccgccataaa accaaacatt
     3181 accaccaagc atttggaaaa tttcgaaaaa gttcatatta aacagctttc actttcatca
     3241 tcgaaggcaa gagttcgtcc ttgatatagg tcgaaatacg cacacgaaca acctcatccg
     3301 ccttttcaac ggaatcaatc gtattcaaac ttatatcagg ctcttggcct aatgcgaact
     3361 ctatagtgcg caaaatatca ggaaaagtaa ttttaccggc caaaaacgcc gccaccgcca
     3421 cttcattcgc cgcattcaac gtaagacaag ccatcggccc cttttccaaa cattcataag
     3481 ccagccgcaa cgccggaaaa cgctccaaat cgggcgtctc aaaattgagc tgtgacattt
     3541 tagccaaatc aagccgctgc ccgggcgtgt tcatccttcc gggccaggcc aaagcataag
     3601 caatcggcgt acgcatatcg ctcgcaccca tttgcgtcag aatagaccca tcagcatatt
     3661 caaccatcga atgcaccacc gattgtggat gaaccaacac atcaatttgc tctgcgggca
     3721 tatcaaacag cacatacgcc tcaatcacct ctaaagcttt attcatcatg ctggcactat
     3781 ccaccgagat ttttgcgccc atatcccagt ttggatgcac cactgcttgc tcaggagtcg
     3841 ccttttcaat ctcttcaaga ctccacgtac gaaacggccc gcccgaagcg gttagaataa
     3901 tccgctgaat atttgcacgc tgcgccggat caaagacctg aaacactgca ttatgctcgc
     3961 tgtcgatcgg caaaattcgt gtattatatt tttccgccgc cgctttcacc aaagctcctg
     4021 ctgccacaag cggctcctta ttcgcaatcg ccacaacctt tgaattttta attgcatgca
     4081 tcaagggcct aagccctgcc atgcctgaaa tcgccgcaat actaagatca gtatcggcct
     4141 gcaccgcttg ttccaaggcc tccggtccgc tcaacacagc aacgccctgc ccctccaaag
     4201 catcgcgtaa aacagcctcg tgcaacggat cggcaataat cgcccggcgc gcctgaagcc
     4261 gcacagccgc cgccgccaac ccttgagcat cttcgttcgc cgtaaccgtg cagacctcaa
     4321 agcgctgcgg cgcagaagca atcacatcgg cgcaactctg tccaatcgaa ccggttaccc
     4381 caaggattga aacacgtttt ttcaatgtct cggcctatag attaaaggag aaaaaggaag
     4441 ttaacacaaa aaacaccggc gccgccaata acagggaatc aatccggtca agcaacccac
     4501 catgcccggg aattaaagct cccgtatctt ttacacccgc cttacgcttc ataaaagaga
     4561 tcagcaaatc gcccccttgc gcaacaatgg caatcataac gccgacaaaa aacataaaaa
     4621 tcaatgctac ccacgagaac accgcctcaa agctaaaggc aatcactccc agataataca
     4681 gtaccgctgc cagtccggca gaatcacacg ctccgcccag ccctgcccag gtcttattcg
     4741 gactaatcag cggcgccagc ttcgctccac caaaatactt gcccgcaaaa taagccccaa
     4801 tatctgctga ccagaccata acgacaaaca agatcgctgc gttcaaagat tgctcctcgc
     4861 gaataacaat acagcacaaa aaaccaactg cgagataaac cagcccccat aaaacatgca
     4921 gcgcagtatc atcgattttg cgcaccatag aaacccactc ataagcggag ataacaaaca
     4981 acagtgcgat catgatataa aatggaattc ctccccatat aacggcaaaa agaacaaccg
     5041 ggataaggac agcagccgaa atagcgcgtt cttgcagggc tttaaggtta aaacggccca
     5101 tttccatcac acctcaatct gcgttaccgc accaaagcgc cgatctcgcc ttgcataaac
     5161 agccaaagca tcttccaaag tacctttgtc aaaatccggc cacagcgtat cggtaaacac
     5221 caattcgcta taagcgcttt gccacagtaa aaaattgctg atgcgctgct cgccgctggt
     5281 acggatcaac aaatcaggat cgggaatacc ttctgtcatc aaaaaaccgg ggaactgggc
     5341 ctcaacagtt tcaaaggaca gcttttgccc caaatgcgca ttgtgcaacc gctgcgccgc
     5401 atgcaaaata tcattacgcc cgccataatt gagcgccatc accaccgtaa tagcgctatt
     5461 atcttcagtg agtttttcgg catgttcaat catccgtaca atatcatcgt caagcgcgtg
     5521 acgatcgcct ataaccctca gctgcgcacc attcttatgc aattccgcca tttctgaacg
     5581 caaataatag cgcagcagat tcatcaaatc cttaatctca ccatcaggcc gactccagtt
     5641 ctcggaagaa aacccgaaca acgtcaaata ttttatgccc agatccattg ccgccttcac
     5701 aacccggcgt gcactttctg caccctgctt gtgcccggcg ctgcgcggca agccccgtgc
     5761 ccgtgcccaa cggccattac catccatgat aatagcaacg tgctttggta tttttatttc
     5821 agtatcttga ggcatattct taaactaaaa cttaaaccgt cataatgtct ttttctttat
     5881 ccgcaagcat cgtatcaatc ttcttgattg cttcgtcggt gagtttctgc acttcatcct
     5941 gcaaccgctt ttcctcatct tctggaagat cgtccttctt caccgcatcc ataccgtcct
     6001 tacgcacatt acgtacagaa atccgtgtat tttcagcata tttaccagca atcttagtca
     6061 gttcactgcg gcgttcttca ttaagatcag gaattggaat gcgtatattg ttaccctcag
     6121 gcatgggatt taagcccaat ccgccatcgc gaatagcctt ttccaccgcc ttcacattac
     6181 cgccatccca aacctgcaca ctcaacatac gcggctcagg cacactcaca gtgccaagct
     6241 gattaagcgg cattttagag ccgtaaacct ctaccaccac aggatcaagc aaatttaccg
     6301 acgcacggcc agtacgcaat ccaccaaact ctttggccaa attatcgatt gcaccatcca
     6361 tccggcgctt aatttcgttt ttattataag acatgacaat aatccctttg ttattcttaa
     6421 ttagtaacga ttgtgaacgt gccttcagaa gccacagctt tggcaaaatt gcccttctcc
     6481 ttcacagaaa acacgataat cggaatatta ttctcccgcg ccagagaaat cgccgttgca
     6541 tccataacct tcaaatcctt ggtcaacacc tccataaaat taatatgcgc ataatgctcc
     6601 gccgtcggat ccttctcagg gtctgcagaa taaaccccgt caactttagt acctttaaaa
     6661 atcgcatcgc agttcatttc cgtcgcacgc aacgccccgg cagtatcggt tgtaaaataa
     6721 ggattacctg tcccggcagc aaaaataacc acccgcccct tttccatgtg acgcaccgcg
     6781 cggcgacgaa tataaggctc gcagattgaa tccatgcgaa tagcagattg cacacgcgtc
     6841 tgcactccaa gattttctag ggcattttgc agcgccaagg cattaataac gatccccagc
     6901 attcccatat aatcagccgt cgtccggtcc attccttcgg ctgcaccggc aactcctcga
     6961 aaaatattac ccgcgccgac cacaacacaa acttcaatgc ttttatcaac aacctcttta
     7021 atatcctcgg caacccgctt aacagtctcc gaatccagcc catactcccc ctcgcccatc
     7081 agaacttccc ccgacatctt aagcatgatc cgcttatagt ctttcatgtt atctttggcg
     7141 gtttgagcat tcatgagctt aggtcctaat ttttgcaaaa ctgttccaat gtaataacag
     7201 ccgcatgctt ttgcaacgcg gctgtcataa aatattaaat tttctcgctt attagccgtt
     7261 cgcagctttt gcaacttctg cagcgaaatc ttcttcttct ttctcgatac cttcaccgag
     7321 ttgaaaacgg gaaaatgctg tcagagtaat tttagagcca gcctcttttg ccgcattcgc
     7381 aatcacgtcg gcaattttgg tttcaccatc aatcactgat ttctggtcca gcaaacaaat
     7441 ctcttcgaaa tatttgcgca tgcgcccttc aaccatcttc tctgcaattt cctgaggttt
     7501 gccttcagcc atagcctgtt caaccaaaaa cgcgcgctct ttttccatct cagccgtatc
     7561 cacggaatta cggtccagat atttcggaaa cgcggcggca acatgcatcg caacctgctt
     7621 gccaagcggc tcaagcacat cagcagacgc atctgattcc agagccacca acacaccaat
     7681 cttacccaac ccctcagcca aagcaccatg cacataagac acgatcgcgc cattgctaac
     7741 ttccagcttc tccgaacggc gcagcgccat gttttcacca atcttggcaa tcagatccgt
     7801 cagagcgtct ttcacagact taccgccgaa atccgatgcc gccaaagcct cgacattttc
     7861 aacatcaagc gctttttcag ccactttttt aacaaaagcc tgaaactctt cattccggct
     7921 tacgaaatcg gtttcagcat taagctcaat caccgcacct tttgtaccat cggttacaac
     7981 agcgaccaaa ccttccgccg ctgtgcgaga agatttctta tcggccttcg ccatcccttt
     8041 tttacgaagc tcttccatag ccgcttccat gtcgccaccg gcttcggtca gagccttttt
     8101 ggcatccatc atgccggcac cggttttctc acggagttcc tttacttttg cagcagtaat
     8161 ctcagacatc ttaacgtcct tcctttgtat attcagattt aaagtattat ctcaaaaaag
     8221 attaagcaga agcttgcttc ttggcttttt cttccgcctc tgcttcatta gcaggctcat
     8281 cagccttgac cacttcagct ttagagtctt ttttcagcgc aggcttcttt ttcttttccg
     8341 catccagctt agcttgctcg cccggtgtca aagcctcagg ctctttatcc ttaacctttt
     8401 tcggcaattt aacctgaacg tcctcagcag cgcccggatc cttgccacca gaagccaatt
     8461 cggcctgaag accatccaaa actgcatccg caatcaaatc acaataaaga tcaatcgcgc
     8521 gcaaagcatc gtcattgcct ggaataatgt aatccacccc ggcaggatcc gcattggtat
     8581 ccacaacgcc aaacacagga atgccaagct tattggcttc tttaaccgcg atgtcttcca
     8641 tcgtcacatc caaaatgaac aaggcatcag gctgcccgcc catatcctta attccgccca
     8701 aagacaactc cagcttgtca cgctcgcgcg tcaacatcaa aatctctttc ttggtacgtc
     8761 ctgcacgctg ctcattgcta ccaagaatat cctgcacttc acgcagacgg ttgatagaac
     8821 cggtaatcgt ctgccagttg gtcatcattc cgccgagcca acggtggttc acatagaact
     8881 gcccacaacg ctttgcgctc tcggcaattt tgtcgcgcgc ctgacgcttt gtacccacaa
     8941 acaaaacgcg tcccccgttc gcaacaatgt cacgaacagc tttcaacgct tcattcagca
     9001 aaggggtcgt ctgttgaaga tcgataatat gaacgccatt acgaacacca aagatatatt
     9061 ggcgcatttt tgggttccag cggcgtgtgt ggtgaccaaa gtg
//
LOCUS       DAOSWR010000083         9076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_4884_length_9076_cov_5.532313, whole genome shotgun sequence.
ACCESSION   DAOSWR010000083 DAOSWR010000000
VERSION     DAOSWR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_9076_cov_5.532313"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(497..1594)
                     /locus_tag="PK513_07025"
     CDS             complement(497..1594)
                     /locus_tag="PK513_07025"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOO82238.1"
                     /translation="MSAQNAALTDDVTNSNSPLLQERHVYKPFLYPWCYEAWLTQQRV
                     HWLPEEVPMAQDVHDWKKTLTPAEKNLMTQIFRFFTQADVEVNNCYMKHYAQVFGPVE
                     VQMMLSAFSNIETIHIAAYSHLLDTLGMPEVEYQAFLKYKEMKDKYDYMQTASMKSRR
                     DIAKTMALFSGFTEGVQLFASFAILMNFPRFNKMKGMGQIVTWSVRDETLHCLSMIKL
                     FKTFISENPDIWDDELKAEITASCRKVIEIEDNFIDLAFEMGGIEGLEPQEVKNYIRW
                     ISDRRLQQLDLEPINAQINKNPLPWMDEMLNGAEHTNFFENRATEYSKASTQGTWEEV
                     FAQDIFSGNYAQGEEAQKIAKDTEDAKGEAA"
     gene            complement(1711..3531)
                     /locus_tag="PK513_07030"
     CDS             complement(1711..3531)
                     /locus_tag="PK513_07030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOO82239.1"
                     /translation="MFDVAQMERGNRVQIDRSRDENLTGFGKATLVDRYLLPEEQPQD
                     LFARVSMHYGDDSEHAQRIYDYISKLWFMPSTPVLSNGGTNRGLPISCFLNETQDSLE
                     EIVELWNENVWLASLGGGIGSYWGNVRSIGEKVGRNGKTSGIVPFIRVMDSLTLAISQ
                     GSLRRGSAAIYLPIWHPEIEEFIEIRRPTGGDPNRKALNLHHGVLVDNRFMHAVENNE
                     EYALKSPKDGHVVDKINARDLWIRLLTARVETGEPYIVYIDHVNDHIPAHHKMAGLNV
                     KMSNLCSEITLPTGVDHLGNDRTAVCCLSSLNLETFDQWQDHPQFIEDVMRFLDNVLS
                     DFIAKAPDSMKRAKYAAMRERSVGLGVMGFHGFLQGKMIPMEGVMAKVWNRKMFSHIR
                     RQADEASVKLADERGACPDAADYGIKERFSNKMAIAPTASISIICGGASPGIEPNAAN
                     AYTHKTLSGSFPVKNKYLMALLEEKGQNTDDVWSSIFTNEGSVQHLDFLSDDEKDVFK
                     TAFEIDQRWLIEHAGDRSEFVCQAQSLNVFLPANVHKADLHGIHYEAWKKGVKSLYYC
                     RSKSIQRAESAASWQRAKEGEDKANEEAQQGQYEECLACQ"
     gene            3932..5362
                     /gene="pyk"
                     /locus_tag="PK513_07035"
     CDS             3932..5362
                     /gene="pyk"
                     /locus_tag="PK513_07035"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOO82240.1"
                     /translation="MSASLKRIRQTKILATLGPSSNTKDQIRELFETGADLFRLNFSH
                     GSHEDIAALAAIIREIESEYNRPIGIVGDLQGPKLRIGTFEKGRIELECGDMIRFDSD
                     PAPGDETRVYMPHSEIFSSLEKDGLFFIDDGKIRCRVREIGQDYFVAEIRAGGTLSDK
                     KGFNVPETFLPIPALTNKDKKDIKAALEIGVDWIAQSFVQTAEDVKEARKLIGGGAAL
                     MIKLEKPAAIQNLEEIVELADGVMLARGDLGVEIPPEDVPSVQKRVVRYVHSQGKPVV
                     VATQMLESMVTSSRPTRAEASDVATAVYDGADAVMLSAETAAGQHPVKSVEMMDRICA
                     RTESDDIYKTMMEETRPDAGDDPSDAITSAAFYVAHDINAACIVTYTASGSTALRMAR
                     QRPDMPVLCLTPHQGVARRMVMSYGVHAVCMPEIKGDFSGPVPYACKILQEEGLAQKG
                     QRFVMTAGVPFGVAGTTNILRIAEVE"
     gene            5493..6260
                     /locus_tag="PK513_07040"
     CDS             5493..6260
                     /locus_tag="PK513_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOO82241.1"
                     /translation="MDSALKTAYSASAVDSDVDLDAVVRKHARLVKNIAYHLQSRLPP
                     NIQPDDLVQAGFIGLMEAAKNYDPSQGASFETYAGIRVRGSMLDEIRRSDWTPRSVHR
                     KARMLAAAEIEVENETGQDALPREIAEKLRISLEEYYKIDQSVAKKNLFSLEQMINPK
                     LETALSAFSEVARDSFKRDLAEAMARLPERERLVIALYYDEELNLREIGEVLGHTESR
                     ACQIHSQATLRLRSRLKDWLSVDSDPLEDTYLQAIPV"
     gene            complement(6427..7353)
                     /locus_tag="PK513_07045"
     CDS             complement(6427..7353)
                     /locus_tag="PK513_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOO82242.1"
                     /translation="MNKVGLFKYFRAALKRRRVGELLVTKGLITPQDLRNALRTHQET
                     RAPLGQVFVEQAAISKTQLRLILLKQTALHTCTALLLCAASLSGGAKKSRADMIKDVP
                     AQIRLVSASSEFKRVASYPAFYGTQEKRSSNLSAFTKWTSMFDRFDRQLKQSASSRIV
                     HEWQENLRGIEGQSLKAMADRVNDLVNEKRYIVDSKNWGQSDYWATPVEFLQRGGDCE
                     DFAIMKYVALRSLGVPEDRMRIAIVQDTQKNIPHAVLVVYTEQGAYLLDNQNKHLISA
                     ERGSRYRPIYSINRQAWWLHSAPSKTIIASAE"
     gene            7563..>9076
                     /gene="rfaE1"
                     /locus_tag="PK513_07050"
     CDS             7563..>9076
                     /gene="rfaE1"
                     /locus_tag="PK513_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOO82243.1"
                     /translation="MTQNLSEETLLPILKKMQDSEVLVIGDVMLDRFVYGYVERISPE
                     SPVPILTIKHEDQMLGGAGNALANLAGLKTKAQFISVIGDDDEGVALKNQIEKLGFSS
                     DGLLIEKTRPTTVKTRFLAGHQQLLRTDFELSAPISDETAALLVETARTALKTARAII
                     LSDYGKGLLRPDVIRAIIEAAKARGVPVLVDPKGQDFSIYRGASALTPNKKELSEATK
                     GAPVSTDDEVLNAAQSLIDECSIKAVVATRSGDGMSVIAKGEAPVHLRTIDIEVFDVS
                     GAGDTVIATIAAGLAAGGTLVQAAALANVAGSVVVTKVGTAPIRSEELAAALAGDRET
                     FGDHAAALLPLAQTIDDITEQQASAFERCLGTRYAPFNPDRDTIRVHPPTKEDRSERY
                     AELERCLTYLLTKANFGRLTDVQIEKAIEAANSSGLRVRLDPSRVEQLDVWVRGLAKT
                     ERRRRTWRKPIKGHPCELEIYRRLVVVSRLKNDPNVLLKMFKEIPVADVEALLP"
BASE COUNT         2360 a         2348 c         2148 g         2220 t
ORIGIN      
        1 catcaaaaca agaagagcgg aaaccagcat ccaggccgta tcaccagaat cgagcgtcgc
       61 gtcctgcgcc agcgccggag tagaagaaaa catcagcgcg ctgacaaaaa gtgccatgac
      121 taaaaatttc acattcaaaa atgccataat gcataccctt caaagctttt tgttttaaaa
      181 acgctgtcga ttttgtaata aaatgctgca ttgcgcaata gcgataatac tgattttttg
      241 aatttgtaca caaaaaagcc gctggaaaca gcggggctat cggacaccgt cattgcaagc
      301 gacccccggc ctccgccggg gcaggctgtg gaagaggagc aatctataaa ttgcatcctg
      361 acgaaagtca ggatctaccc gaaggagatc ccaaatcaag tttgggatgc attcttaccg
      421 gcttgcttcg cggccttcct cgcaatgacg cctcaagggg agtattgatt ggccccgaaa
      481 gagaacaaag aaagacttaa gcagcctctc ccttggcatc ttcagtatcc ttggcgatct
      541 tctgtgcttc ctcgccctgc gcataattgc cggagaaaat atcctgcgca aaaacctctt
      601 cccatgtgcc ttgcgtagaa gctttggaat attccgtcgc gcggttttca aagaagttcg
      661 tgtgctcggc gccattgagc atttcatcca tccatggcag cggattttta ttaatctgcg
      721 cattgatagg ctcgagatca agctgctgca ggcggcggtc tgaaatccag cggatatagt
      781 ttttcacttc ctgtggctcc aacccttcaa taccgcccat ttcaaaggcc aaatcgatga
      841 agttatcttc gatctcgatg actttacggc aagaggcggt gatttccgcc ttcaattcat
      901 cgtcccagat gtccgggttt tcagagatga aggttttgaa cagcttgatc atcgacaggc
      961 agtgcagcgt ttcatcgcgc accgaccatg tcacaatctg gcccatgcct ttcatcttgt
     1021 taaagcgtgg gaagttcatc aaaatcgcaa acgaggcaaa gagctgcacg ccttcggtaa
     1081 agccggaaaa cagcgccatc gttttggcaa tgtcgcgacg cgatttcatc gaagccgttt
     1141 gcatatagtc gtacttgtcc ttcatctcct tgtatttgag gaaggcctga tattcgacct
     1201 caggcatacc cagcgtatca agcaggtgcg aataggccgc gatgtgaatg gtttcgatat
     1261 ttgaaaaggc gctaagcatc atctgcacct caaccgggcc gaacacttgc gcataatgtt
     1321 tcatatagca gttattgacc tcaacatcgg cttgggtaaa aaagcggaag atctgcgtca
     1381 tcaggttttt ctcagccgga gtcagcgttt ttttccagtc atgaacgtcc tgcgccatcg
     1441 gcacttcttc cggcagccag tgtacgcgtt gctgggtcag ccatgcttca taacaccagg
     1501 gatacaagaa aggcttgtag acatggcgtt cttgcaacaa cggggaattg gaattggtga
     1561 catcatcggt aagggcggcg ttttgcgcag acataaagag tatccttttt aaaataagtt
     1621 gaagatttgc atatactaag aatgattgcg ttttgcaagc ttgccgcgcc ctggcaacaa
     1681 aaaaccataa tcttggtcaa agaaaaactt ctactgacac gcgaggcatt cttcatactg
     1741 gccttgctgg gcttcttcat tagccttgtc ttcgccttct ttcgcccgct gccaactggc
     1801 cgcgctttcc gcgcgttgaa tggatttaga gcggcagtaa tacaaagact ttacgccctt
     1861 cttccatgct tcatagtgaa tgccgtggag atcagccttg tgaacattgg ccggcaggaa
     1921 cacattcaaa gattgcgcct ggcagacaaa ttcggagcga tcgcccgcat gctcaatgag
     1981 ccagcgctga tcaatctcaa acgcggtttt aaacacgtct ttttcatcgt ctgaaaggaa
     2041 atcgagatgt tgcaccgacc cttcattcgt aaaaatggaa ctccacacat catcggtgtt
     2101 ttgccctttt tcctccagca acgccatcag atatttattt ttcaccggga aagaacccga
     2161 cagcgtttta tgcgtgtaag catttgccgc attgggttca atgcccggag acgcgccgcc
     2221 gcaaataatg gagatgctgg ccgtgggtgc gatggccatt ttgttggaaa aacgctcctt
     2281 aatgccgtaa tcagctgcgt ccggacatgc gccgcgttca tcggccagtt ttacagatgc
     2341 ttcatcagct tgacgacgaa tatgagaaaa cattttgcgg ttccacacct tcgccattac
     2401 accttccatc gggatcatct tgccttgcag gaagccgtgg aagcccataa cgcccaggcc
     2461 aacgctgcgc tcgcgcattg cagcatattt cgcgcgcttc attgaatcag gcgctttagc
     2521 gataaagtcg ctcagaacat tatcaaggaa gcgcattacg tcttcgataa attgcggatg
     2581 atcctgccat tgatcgaatg tctcaaggtt caaggagctt aaacaacaca ccgccgtacg
     2641 gtcattgcca agatggtcca cccctgtcgg caaagtgatt tccgaacaca gattggacat
     2701 tttcacattg agacccgcca tcttatggtg cgccggtatg tggtcattca catgatcaat
     2761 ataaacaatg tacggctcgc cggtttccac ccgggcggtc aatagacgaa tccaaagatc
     2821 gcgcgcattg attttatcaa cgacatgacc gtctttcggg gattttagcg cgtactcctc
     2881 attattttcg accgcatgca taaaacggtt atcaaccaaa acaccatgat gcaggttcag
     2941 agctttacgg ttcggatccc cgccagtcgg gcggcgaatc tcaataaact cttcaatttc
     3001 cggatgccag atcggcaggt aaatcgcagc agagccgcgg cgcaacgagc cctggctaat
     3061 cgccagtgtt agggaatcca taacacggat aaagggaaca atgccgcttg tcttgccatt
     3121 gcggccaacc ttttcaccaa tagagcgtac attgccccag taagaaccaa taccgccgcc
     3181 caaagaggcc agccagacat tctcattcca caattctaca atctcttcga gactatcctg
     3241 tgtttcattc aagaagcagg aaatcggcaa accacggttt gtgccgccat ttgacaaaac
     3301 aggcgttgaa ggcataaacc ataattttga gatataatca tatatgcgct gtgcatgttc
     3361 gctatcatcc ccataatgca tcgaaacgcg cgcaaacaaa tcctgaggtt gttcttccgg
     3421 caacaaatag cgatcaacca gagttgcttt accaaaacca gtcaggtttt catcccgaga
     3481 gcggtcaatc tggacacgat tgcccctctc catttgcgca acgtcaaaca taagccttac
     3541 tcctattata agtcttcttt tatccataaa ggacggggtt ttatggggtg cagacagcgt
     3601 gtgaagacac tatctatagc gttcgttaag agataagcta ctacatctcg tggtgtgcag
     3661 caataggaat atggtcctaa aaacagttgc aattttttta aaaatctttt tctttcaata
     3721 aattaacagg gatatccgca gccttatccc cgtggaaaaa catgatgttt ttttacaaac
     3781 gtaataaaat tacacaaaag ccccgaaatt tctgcgggta atttgcacat aatacccgtg
     3841 atgttcttac tgtgttctgg ctctggtgcg atgtcaaata tatgttatga ttcttttgaa
     3901 aattcaccaa gcctgacgct aggaaatcct tatgagcgcc tctcttaaac gcatccgtca
     3961 aactaaaatt cttgccacat taggaccatc aagcaacacc aaggaccaga ttcgcgaact
     4021 atttgaaacc ggcgctgacc tctttcgtct caatttcagc cacggcagcc atgaagacat
     4081 agcagcgctg gcggctataa tccgtgaaat cgaatctgaa tataaccgcc cgattggcat
     4141 tgtcggcgac ctgcagggcc ccaaactcag aattggtaca tttgaaaagg gccggatcga
     4201 acttgaatgc ggcgatatga ttcgctttga ttctgatcct gccccgggtg atgaaacgcg
     4261 ggtttatatg ccgcattcgg aaattttctc cagtttggaa aaagacggcc tgttctttat
     4321 tgatgacggc aagatccgct gtcgcgtacg tgaaatcggg caggattatt ttgtagctga
     4381 aattcgcgcc ggcggaacgc tttcggacaa aaaaggcttt aatgttcctg aaacctttct
     4441 gccgattcct gctttaacaa ataaggataa aaaggacatc aaggcggctc ttgaaattgg
     4501 cgttgactgg atagcacaaa gtttcgtgca aaccgccgag gatgtaaaag aagctcgtaa
     4561 gcttattggc ggcggtgcag ccctgatgat caaactggaa aaaccggctg ccattcaaaa
     4621 tctggaagag attgtcgaac tggctgacgg agttatgttg gcgcgcggcg atcttggcgt
     4681 tgagattcca ccagaagacg ttccatctgt tcaaaaacgg gttgtgcgtt atgtgcatag
     4741 ccagggcaag ccggtcgttg tcgccacgca gatgctcgag tcgatggtga cctcttcccg
     4801 gccgactcgc gccgaagcct cagatgtggc tacggccgtc tatgatggcg ctgatgcggt
     4861 gatgctttca gctgaaaccg ccgccggcca acaccctgtg aaaagtgtcg agatgatgga
     4921 ccgcatctgc gcacgcaccg aaagcgatga catctataaa accatgatgg aagagacacg
     4981 cccagacgct ggcgatgatc cctccgatgc catcacctcg gcagcttttt acgtcgccca
     5041 cgatattaat gccgcgtgca ttgtgactta tacagcctca ggctcgaccg ccctgcgcat
     5101 ggcccgccag cgccccgata tgcctgtctt gtgcctgaca ccgcatcagg gcgttgcgcg
     5161 gcgaatggtt atgtcttacg gcgttcacgc agtatgcatg ccagagatta aaggtgattt
     5221 ttctggcccc gtgccctatg cgtgcaaaat tttgcaggaa gaaggtcttg cccaaaaagg
     5281 ccagcgcttc gtcatgaccg ccggcgtgcc tttcggcgtt gccgggacga cgaatattct
     5341 cagaattgcg gaagtggagt aaagaacatt caaatcggaa ggagcttttc tataaaaaaa
     5401 gtatatttat acaataaatt aagcttgata taataaaaaa atattctatt gtgcatccca
     5461 taattttaac agttatgcaa atgggaagga caatggacag cgcattaaaa actgcatact
     5521 cagcttcagc cgtagattct gacgtcgatt tagacgccgt ggttcggaaa cacgcccggt
     5581 tggttaaaaa tatagcctac catcttcaat caaggcttcc tccaaacatt cagccagacg
     5641 atctcgtcca ggccggtttc atagggttaa tggaggcagc aaagaattac gatccttcgc
     5701 aaggcgcctc ttttgaaacc tatgccggta tccgcgtccg cggttccatg ctggacgaaa
     5761 tccggcgctc tgactggacc ccgcgctccg tccatagaaa agctcgaatg ctcgcggcgg
     5821 cggaaattga agttgaaaat gaaacagggc aggacgcact tccccgagaa atcgcggaaa
     5881 aactgagaat ctccctggag gaatactaca aaatcgatca gtcggtcgcc aaaaaaaatc
     5941 tttttagtct ggagcaaatg ataaatccca aattagagac agccctctct gccttctccg
     6001 aagttgccag agattcattt aaaagagatc tggccgaggc tatggcacgc ttgcctgaaa
     6061 gagaaagact tgttattgcc ctgtattacg atgaagagtt gaatttaaga gaaatcggcg
     6121 aggttttggg gcataccgaa tccagagcat gccagattca ttcacaagcg accctgcgac
     6181 tcagatcacg ccttaaggat tggctaagtg tggattcaga cccattagaa gacacatatc
     6241 tacaggctat acccgtctaa ccgcaaggct ttatctcttt caggtttgat tcctcgccgc
     6301 ttacggcgta atcgtcatcg cgagcgaagc gaagcgatcc agtgccacaa ggataaattc
     6361 tggattgctt caccgccttt ggcggttcgc aatgacgtga tacctcgcct cttggggcgg
     6421 gggtgttcat tccgcgctgg cgataatcgt tttactcggt gcgctatgca gccaccaggc
     6481 ctgacggtta atggaataaa tcggacgata gcgtgatccg cgttccgcgc taatcagatg
     6541 tttattctgg ttatccagca gataggcgcc ctgctctgta taaaccacca aaactgcatg
     6601 cggaatattc ttctgcgtat cttgtacgat agcgatgcgc atccgatctt ccggcacacc
     6661 aaggctgcgc agcgccacat atttcatgat tgcaaaatct tcgcaatcac cgccgcgctg
     6721 gagaaactca accggcgtgg cccagtaatc ggactggccc cagttttttg aatccacgat
     6781 gtaacgcttt tcattcacca gatcattcac ccggtcggcc atggctttca ggctttgccc
     6841 ttcaatgccg cgcagatttt cctgccactc atgaacgata cgagacgatg cgctttgttt
     6901 taactgccga tcaaaacgat cgaacatgct cgtccacttc gtaaaagcgc tcagattgct
     6961 tgagcgcttt tcttgtgtgc cgtaaaacgc cgggtaagag gcaacacgtt tgaattcact
     7021 gctcgcactt accaagcgaa tttgcgcggg cacatctttg atcatatcgg cgcgtgattt
     7081 ttttgcacca cccgaaagcg aagccgcaca caatagtaaa gccgtacaag tatgcaaagc
     7141 cgtttgtttc aataaaataa gacgcaattg tgttttggaa atggccgctt gttcaacgaa
     7201 aacctgcccc agtggagcgc gcgtttcctg atgcgtacgc aaggcgttgc ggagatcctg
     7261 aggcgtgatc aatcctttgg taaccagcaa ctcaccgacc cgcctacgct ttagcgcagc
     7321 tcgaaaatat ttgaaaaggc ccaccttgtt catagacttt atcccttttt aagaccctgc
     7381 tctcatcctt gtttttcatt tttgtttaaa gcaataggta cacacgtaaa aattcgtacc
     7441 cccttattaa acctcacaaa gattgacgaa ttcctaacaa acgggcataa aagcgaggtt
     7501 ttcaggatat ttttacgcaa aaactcagaa aaacagaaca aaataagaat atggaaaatt
     7561 ttatgaccca aaatctatct gaagaaacac tgttgccaat tcttaaaaag atgcaagatt
     7621 ctgaagtttt ggtgattggc gatgttatgc ttgatcgctt tgtttacggc tatgttgaac
     7681 gcatttctcc cgaaagtcct gtgccgattt tgaccatcaa gcatgaagat caaatgctgg
     7741 gcggggccgg gaatgccttg gccaatttag ccggattaaa aacgaaggct cagtttatct
     7801 ctgtgattgg agatgatgat gaaggcgttg cgcttaaaaa ccaaattgaa aaacttggtt
     7861 tttccagcga tggtttgctg attgaaaaaa cccgtccaac aactgtgaaa acccgttttc
     7921 tcgccggaca tcagcaactc ttgcgtacag attttgagct cagcgctccg atcagcgatg
     7981 aaaccgccgc tctgctggtt gaaacggcaa gaacggccct taaaacggcc cgcgccatta
     8041 tcctttcaga ttacggcaaa ggcctgctgc gcccagatgt gattagagcc ataatagaag
     8101 cggcgaaggc gcgtggcgtt cctgtccttg tcgaccctaa aggacaggat ttttctatat
     8161 accgcggtgc cagcgcgctc acccccaata aaaaagaact tagcgaggca accaaaggtg
     8221 cgcctgtaag caccgatgat gaagtgctaa acgccgctca aagtttaatt gatgaatgca
     8281 gtataaaggc cgtggttgca acccgctccg gcgatggtat gagcgtcatt gctaaaggcg
     8341 aagcgcctgt acatttacgc acaatcgaca tcgaggtgtt cgatgtttcc ggcgctggcg
     8401 atactgtaat tgccacgatt gctgctggtt tggcggcagg cggaacgctg gttcaagccg
     8461 ccgcgcttgc caatgttgcg ggctctgttg ttgtcaccaa agtcggaacc gcaccaattc
     8521 gtagcgaaga actcgccgcc gcactcgccg gcgaccgcga gaccttcggc gatcacgccg
     8581 ccgccttgct gcccctcgcc cagaccatcg acgacatcac cgagcaacag gcctccgcct
     8641 tcgagcgttg cctcggcaca cgctacgccc cgttcaatcc cgaccgcgac accatccgcg
     8701 tccacccgcc gacgaaagag gaccgctccg agcgctacgc cgaactcgaa cgctgcctga
     8761 cctacctgct gacaaaggcg aatttcggcc gcctgaccga tgtgcaaatc gaaaaggcaa
     8821 tcgaggcggc aaactcatcg ggcttgcgcg tacgactcga tccgtcgcgt gtcgaacagc
     8881 tcgacgtctg ggtccgcggc ttggccaaaa ccgaacgccg ccgccgcacg tggcgcaagc
     8941 caatcaaggg ccacccctgc gagctcgaga tttaccgccg cttggtcgtc gtctcgcgtc
     9001 tgaagaacga cccgaacgtg cttctgaaga tgttcaagga aatccccgtc gctgacgtcg
     9061 aagcactatt gccgca
//
LOCUS       DAOSWR010000084         8877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5045_length_8877_cov_5.471662, whole genome shotgun sequence.
ACCESSION   DAOSWR010000084 DAOSWR010000000
VERSION     DAOSWR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_8877_cov_5.471662"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK513_07055"
     CDS             <1..526
                     /locus_tag="PK513_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO82244.1"
                     /translation="TVNGISKAYAMTGWRIGYAGGPKDLIKAMAKVQSQSTSNPCSIS
                     QAAAVAALNGDQSFLAERNAVFKQRRDLVVDMLNEADGISCITPEGAFYVYPSCAGCI
                     GKTTPDGKVIESDEGFVTYLLESEGVACVHGEAFGLSPHFRISYATSTEALTEACKRI
                     QRACAALQGEAKAA"
     gene            complement(531..1733)
                     /locus_tag="PK513_07060"
     CDS             complement(531..1733)
                     /locus_tag="PK513_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO82245.1"
                     /translation="MKTNQNTVVVLFNYGGGMRGLIPAHLMSKIEEVTGLPMADMVDI
                     FAGPSTGAILNAALNIPHDDDPLRPKWRARHMVKFYEREGIKIFPRDRFRDFRAFVHD
                     FNNRTMKIGQLQGLMRKGHYDPSYLSYCLRGLYGKRTLSETLKSLIIPVYNIDGEQLD
                     LVEEAGETDNTPVHTKNNFIDGGGHAVWLKNIQQRRNQRKTPDVELHDAILASAAAPT
                     YFPCHHFKSQNPATGKIVEYSGIDGSIFDNPCVSYHGAIRQHLDAGTRVIMILLGTGY
                     THRSFKKEDWNSYGGLGVVDPVNDLPLINILFHAPESALIESFRQELGDDLFVFNKSL
                     VADIGKPDAVNMAIDDAGSENLKRMSHFSEAILEEQSQEFDRLCHILVRNRDSKPQKR
                     SFWSRLLD"
     gene            complement(1830..3305)
                     /locus_tag="PK513_07065"
     CDS             complement(1830..3305)
                     /locus_tag="PK513_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOO82246.1"
                     /translation="MSALGVFVVLYMVISIAIGLYASLRVRTSTDYVLAGRSLPIYVT
                     IATVFATWFGSEAVLGMPATFMEEGLGGIVADPFGAGLCLVFVGLFFAARLYRMKLLT
                     IGDFYRQRYGKTVEILVSLAICVSYLGWVSAQIVALGLTISLISGEAISFEVGMLIGL
                     SIVLLYTIFGGMWSVAIMDFLQMMMILMGLLIVAWLIAGRVDGGFMGVVHDAAANNKF
                     DFWPDLNPAAILAFIGAFVTLALGSIPQQDVFQRVMSAKDEKTAVRGTVIGGTFYIFF
                     CFVPIFIAYGATLIDPNLVTTHMGEGGDPQRILPEFILNGVPMPIQVMFFGALLSAIM
                     STASGTLLAPSAILAENIFKEILVSKLDDKHLLTVLRICVFCFGLVVLAYAYLSTSAG
                     LSIFEMVENAYLVTLCGAFVPLAFGVYWKKATNAGALLSIACGVITWSVLEYLNVRMA
                     AQDESMLVPPQLAGLFMAIFGMIAGSLLPQINPPKAEKQPS"
     gene            complement(3359..4600)
                     /locus_tag="PK513_07070"
     CDS             complement(3359..4600)
                     /locus_tag="PK513_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOO82247.1"
                     /translation="MTDDTFQGSLEASALTFSHSDQISFDDIPPRSGAADIDSDVIDD
                     YIRTLRPTVPVAILKPQKLSQRAKEFTKSFTGCALYAVKCNPDEVLLKTMYANGVRRF
                     DVASISEVRLIRGLFPRAKIYFMHPIKAPEAIHEAYINHKVRAFVLDYADELDKILYE
                     TNAAADLELFVRLAVPKDNQGSDVATDFSSKFGAKPEYAAELLRLCRPYCKKLGLSFH
                     VGTQCQDPSAYSKAIAHAANVIEESGVSVDVLDIGGGFPAELDTENPMAPIQAFTREI
                     EGAVRVNGLKELELLCEIGRGLVACAGALVVRVEGRKDDLLYLNDGTYGGLFEAGGSI
                     GLAYPAHLIRREERSFEGPVKPFRFAGPTCDSVDMMKGPFMLPADVQTGDWIRLEQLG
                     AYGEVSRTDFNGFGSARKIVV"
     gene            complement(5017..5991)
                     /gene="trxB"
                     /locus_tag="PK513_07075"
     CDS             complement(5017..5991)
                     /gene="trxB"
                     /locus_tag="PK513_07075"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOO82248.1"
                     /translation="MNETHHTKVLIIGSGPAGYTAAIYAARANLEPILVTGFEPGGQL
                     MITTEVENYPGFADVIQGPWLMEQMKLQAEHVGTTLINDYIKEVDFSTRPFKAIGDQG
                     TVYTGDSVIISTGAKARWLGLPSEEKFRGKGVSACATCDGFFFRGKDVAVVGGGNSAV
                     EETLFLTNFCSKVTLIHRRNSLRAEKIMQDRLFENEKVEVIWDSTVEEVLGDDSGMTG
                     LRLKNVKTSEESTLEADGMFVAIGHDPATEIFKGQIKMDEAGYIDTAADSTATSVPGI
                     FAAGDVADHVYRQAVTAAGMGCMAALEADRFLAVQHTPNNRNPAHAAE"
     gene            complement(5988..6938)
                     /locus_tag="PK513_07080"
     CDS             complement(5988..6938)
                     /locus_tag="PK513_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HOO82249.1"
                     /translation="MMQELSKNTAKNITCWVVTEGLAGTENQCLGVAEALGIVPEVKR
                     IELRQPWKSLSPYLGFEQNWSFSPALTGPWPDLLLASGRKSIAASRYIKKASGGKTFT
                     VQIQDPRIFPKQFDLVAVPAHDPTRGENVIVTTSAPNRVTPKKLETAKAEFSQFGKLS
                     TPRVAVLIGGSSKAYGMTSEITRRLAAQLKNIDASLMITASRRTGAENEAILRRELGN
                     HYFWNGQSENPYFGLLAWADFILVTADSASMLSEACTTGKPVYMIPLEGGHPRIDKLH
                     ENLKQSGCLRDFDGNLEGWLYEPLNDADLIAHEIRKHIKL"
     gene            7008..7481
                     /locus_tag="PK513_07085"
     CDS             7008..7481
                     /locus_tag="PK513_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOO82250.1"
                     /translation="MRRSKLDAIDKKILQNLQADGRMTNVELAKVVGISAPPCLRRVR
                     ALEEAGYIHSYHAQIDPAILGYSVTVYAMVRLESQAEKDLEAFEKHMVELPMVRECHM
                     LAGDVDFILKIVAKDWDSYQEFLTHELTTAPNVVSVKSSLNIRSAKDEAGVPIEQ"
     gene            complement(7489..7962)
                     /gene="greA"
                     /locus_tag="PK513_07090"
     CDS             complement(7489..7962)
                     /gene="greA"
                     /locus_tag="PK513_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019953512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOO82251.1"
                     /translation="MEQIPVTKIGFEGLEAELKDLKTVQRPNVIEAIATAREHGDLSE
                     NAEYHAAREQQSFIEGRIKELEAVIGRAQVIDPSTLSGSAVKFGATVTIVDEETDEES
                     TYQIVGDYEADLAHSKISISSPIARGLIGKSEGDSVVIQTPKGKGDYEILAVKYK"
     gene            complement(8170..8544)
                     /locus_tag="PK513_07095"
     CDS             complement(8170..8544)
                     /locus_tag="PK513_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82252.1"
                     /translation="MSLFVYKFGGAIKTAFAISADLDSSDSGQNATIIHHSIPNAHRT
                     ACDKQDIVNIPVDAFEKFTEGVMAAGGQSKILVLDTTYDKTKDRRDPDAYSGGQKAMH
                     HAYNNGYDAFRSFIDEHTPNRV"
     gene            complement(8613..>8877)
                     /locus_tag="PK513_07100"
     CDS             complement(8613..>8877)
                     /locus_tag="PK513_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82253.1"
                     /translation="GQPHVLDAMINGEIALVINSTTKSSQAVADSSSIRRMALMNKIP
                     YYTLLTAASAAVKAIAAIKAKDIEVAPLQEFFPVGAKAKKDAA"
BASE COUNT         2333 a         2316 c         2074 g         2154 t
ORIGIN      
        1 gaccgttaac ggcatatcca aagcctacgc aatgaccggc tggcggatcg gttatgccgg
       61 cgggccgaaa gatttgatta aggccatggc caaagttcaa agccagagca cctccaaccc
      121 gtgctcaatt tcccaagctg ccgctgtggc cgcgctgaac ggcgatcaat cttttctcgc
      181 cgagcgcaac gccgtattta agcagcgccg cgatttggtg gttgatatgc tcaatgaggc
      241 cgacggcatt tcctgcatca cccccgaagg cgcgttttat gtctatccct cgtgcgcggg
      301 ctgcatcggt aaaacgacgc cggacggcaa ggtgatcgaa agtgatgaag gcttcgtcac
      361 ctatttgctg gaaagcgaag gcgtcgcgtg tgtgcatggc gaagccttcg gcctgtcgcc
      421 gcatttccgc atctcctatg cgacatcaac cgaggcactc accgaggcct gcaagcgcat
      481 ccagcgcgcc tgcgcggcat tacaaggcga ggcgaaggca gcttaaacat ttagtccaac
      541 aaccgggacc aaaaactgcg tttttgtggc ttagagtcgc ggttacgcac caaaatatga
      601 cagagccggt caaattcctg gctctgttct tctagaattg cctcagagaa gtggctcatc
      661 cgctttaaat tttctgatcc ggcgtcgtca atcgccatgt tcaccgcatc cggcttgcca
      721 atatcggcca ccagagattt gttaaataca aacaaatcat cgcccagctc ttgccgaaaa
      781 ctttcaataa gcgccgattc cggcgcatgg aacaaaatat tgatcagcgg tagatcattc
      841 accggatcaa caacgccaag cccgccatag ctgttccagt cttctttttt gaatgaacgg
      901 tgcgtatagc ccgtgcccag caaaatcata atcacccgcg ttccagcgtc caaatgctgg
      961 cggatagccc catgatagga aacgcaaggg ttgtcgaaaa tgctgccgtc aatgccggaa
     1021 tattcaacga ttttgcctgt ggccggattt tggcttttaa aatgatgaca agggaagtat
     1081 gtcggcgccg ccgcactggc caatatggca tcatgcaatt cgacatccgg cgttttgcgc
     1141 tgattacggc gctgttgaat gtttttgagc catactgcat gcccgccgcc atcaataaag
     1201 ttatttttcg tatgaaccgg cgtattgtcg gtttctccag cctcttcaac caaatcaagc
     1261 tgttcgccat caatattata aaccggaata ataaggctct taagtgtttc ggaaagcgtg
     1321 cgcttaccgt acaatccgcg caggcaatag gaaaggtaag acggatcgta atgccccttg
     1381 cgcataagcc cctgcaactg gccgatcttc atcgtccgat tgttaaaatc atgcacaaag
     1441 gcgcggaaat cccgaaagcg atcgcgtgga aaaatcttaa tgccttcgcg ttcgtaaaat
     1501 ttcaccatat gccgcgcccg ccatttgggc cgcaaagggt catcatcatg tggaatattg
     1561 agcgccgcat tcaagatagc gcccgttgaa ggtccggcga aaatatcaac catgtctgcc
     1621 ataggcaggc cggtaacctc ttcgattttc gacataagat gcgcggggat aaggccgcgc
     1681 atcccgccgc catagttaaa caggacaaca acagtatttt gattggtctt cacagctaca
     1741 aaaactctct acttctgaaa ataaaagcca gaatagaatt ttttgagccg atccgaaacc
     1801 attaaaatgc gtttgtaata gtattggaat tagctcggtt gtttctcagc ttttggcgga
     1861 ttgatctgtg gcagcagact accggcgatc atgccaaaaa tcgccataaa cagtccggcg
     1921 agttgcggcg ggaccagcat ggattcgtct tgcgcggcca ttcgcacgtt gagatattcc
     1981 agcacagacc aggtaatgac gccgcaggcg atggaaagca gcgccccggc attggtggct
     2041 tttttccagt atacaccgaa ggctagcggc acgaaggccc cgcacaacgt aacgagataa
     2101 gcgttctcga ccatctcgaa aatacttaag ccagctgatg tacttaagta agcgtaggcc
     2161 agtacgacca gaccgaaaca aaaaacgcag attctcagga cagtaagcag atgcttatcg
     2221 tcgagtttgg agaccagaat ctccttgaaa atgttttcgg ccagaatcgc cgaaggcgcc
     2281 agcaacgtac ccgaggctgt agacatgatc gccgaaagaa gagcaccaaa gaacatgacc
     2341 tgaataggca tcggtacgcc gttcagaata aattcaggta agatacgctg cggatctccg
     2401 ccttcgccca tatgcgttgt gaccaggttc ggatcaatca gggttgcgcc ataggcgata
     2461 aaaattggca caaagcaaaa gaagatatag aacgtgccgc caatgacagt gccgcgcacg
     2521 gcggtctttt cgtctttcgc cgacatgacg cgctgaaaga cgtcctgttg cggaattgag
     2581 ccgagcgcca gcgtcacgaa tgcgccgatg aaggcgagga tagccgccgg gttcagatcc
     2641 ggccagaaat cgaatttatt gttagcggcg gcgtcgtgaa cgactcccat aaagccgcca
     2701 tcgacccgtc cggcgattaa ccaagcgaca atcagcaatc ccatgagaat catcatcatt
     2761 tgcaggaagt ccatgatcgc aaccgaccac atcccgccga agatggtata aagcaggacg
     2821 atgctcaggc cgatgagcat gccaacctca aaggaaatag cttcaccaga gattaagcta
     2881 atggtcagtc ccagcgcgac gatctgggct gaaacccagc cgagatagct gacacagata
     2941 gcaagagaaa ccagaatctc gacagtttta ccgtagcgtt ggcggtagaa gtcgccaata
     3001 gtcagcagtt tcatgcggta gagcctcgcg gcgaagaata ggccgacaaa aaccaggcac
     3061 agccctgcac cgaacggatc ggctacaatt ccacccagcc cttcctccat gaaggtcgca
     3121 ggcatgccga gtacggcttc cgagccaaac catgttgcaa agactgtagc aatggttaca
     3181 taaatgggga gcgagcgccc ggcaagaaca taatcggtgg aggtcctgac tcgcagcgaa
     3241 gcatacagac caatcgcgat tgagatcacc atatacagaa cgacaaaaac gccgagtgcg
     3301 ctcattttat ttccccacta gttattttcc ttgttactcc tacgccgtga gattcatact
     3361 acacgacgat tttccgagcc gatccgaaac cattaaaatc tgttcgtgaa acttcaccgt
     3421 aagcgcctaa ttgctctaac ctgatccagt caccggtttg aacatcggca ggcagcataa
     3481 aagggccctt catcatatca acagaatcgc aggttgggcc tgcaaaacgg aaaggcttaa
     3541 cggggccttc aaaagaacgc tcttcacggc ggatcaaatg cgcagggtag gcaaggccaa
     3601 tggagccgcc ggcttcaaac aaacctccat aagtgccgtc attaaggtaa aggagatcgt
     3661 ctttgcgccc ctcaacacgt acaacaagag cgccagcaca ggcaacaagc ccgcgtccga
     3721 tttcgcacag cagctcgagt tccttaagcc cgttcacacg cacagcaccc tcaatttctc
     3781 gtgtaaaagc ctgaataggg gccatcggat tttctgtatc taattcagca ggaaatccgc
     3841 cgccaatatc gagaacatca acactaacac cactttcctc aataacattt gcggcatggg
     3901 caatagcttt cgaatacgcg gaagggtctt ggcactgtgt tccaacatga aaacttaaac
     3961 ccagcttctt acaataagga cggcagagtc tgagtagttc ggcagcatat tcaggcttgg
     4021 ctccaaattt gctggaaaaa tcggtcgcca catcgctgcc ttggttgtcc ttcggtacgg
     4081 ccagtcgtac aaaaagttca agatccgccg ctgcattggt ttcataaaga attttgtcca
     4141 gttcatcggc ataatccaga acaaaagccc ttaccttatg gtttatatag gcttcatgaa
     4201 tagcttccgg agccttgata ggatgcataa aataaatctt agcccttgga aacagtcctc
     4261 ttatgagacg aacttcgcta atagaagcca catcaaaacg acggactccg ttcgcataca
     4321 tggtttttag caagacttca tccggattgc atttcacggc ataaagagcg cagccggtaa
     4381 acgatttggt aaattccttg gctctttgcg aaagtttttg cggttttagg atagccacgg
     4441 gaacagtagg gcgtaaggtt ctgatataat cgtcgatcac gtcagaatca atgtctgcag
     4501 ccccggagcg cggcggaata tcatcaaaag aaatctgatc tgaatgcgag aatgtaagtg
     4561 cggaagcctc cagacttccc tggaaagtat cgtcggtcat caacggtaaa ccctatgcta
     4621 aaggaggcaa agcctcaagt taactacaag atatacgtcg ttgcataacc gacatagggc
     4681 cataggcacg tgcgtacttc cttatgatac aaaaatacca aaaaagcaat atttaattta
     4741 acgtcaacat agtaaaaaaa accgggcaac cactacaaaa tatagtgggc ccggcgagtt
     4801 gaacagggag gtttcacgtc tgggagacgt aaggatctgt tacgaccctt atcaacatag
     4861 tgttgatccc ggggttatag gacatctcgc ctgcgttttc aacatcttca gaaggaaaaa
     4921 atatgtcgtt ttttactcga aaaacgttac tttacttcgt tgatagcaat taaaaacgag
     4981 gcggtgcgga atgttttggg tgtattttat gctgttttat tccgctgcat gtgcaggatt
     5041 tctgttgtta ggagtatgtt gcacggctaa aaaccgatcg gcttccaacg cagccatgca
     5101 gcccatcccc gcagctgtga cagcttgacg atagacgtga tcagcaacgt ctccggcagc
     5161 aaaaatgccc ggtacagaag tcgctgtaga atctgcagcc gtgtcgatat agcccgcctc
     5221 atccatcttg atctggcctt taaaaatctc ggtcgccgga tcatgaccaa tcgccacaaa
     5281 cattccatca gcctccagcg tactctcttc actggtttta acgttcttga ggcgtagacc
     5341 tgtcattccc gaatcatcac ccagaacctc ttcaacggtg ctgtcccaaa ttacttcgac
     5401 tttttcgttt tcaaataaac gatcttgcat gattttctcg gcgcgcaaag aattacggcg
     5461 atgaattagc gtaactttcg aacagaaatt cgtcaggaat aatgtctctt cgaccgcaga
     5521 gtttcctccg ccgacaaccg caacgtcttt accgcggaag aaaaacccat cgcacgttgc
     5581 acacgccgaa acgccttttc cacgaaactt ctcctctgat ggcaggccaa gccagcgagc
     5641 tttcgctccg gtagaaataa taacgctatc acctgtataa accgtgccct gatcaccaat
     5701 tgctttaaac ggacgtgtag aaaaatcgac ttcctttata taatcattaa tcagggttgt
     5761 gccgacatgc tcggcctgaa gcttcatttg ctccatcagc cacggcccct gaatgacatc
     5821 ggcaaaaccg ggataatttt caacttccgt ggtgatcatt aactgtccgc ccggctcaaa
     5881 cccagtcact aaaatcggct ccagattagc gcgtgccgca taaattgccg ccgtgtaccc
     5941 cgctgggccg gagccgataa tcaaaacttt tgtatgatgc gtttcattca taattttata
     6001 tgctttctaa tttcatgggc gatcaaatca gcatcgttaa gaggttcgta caaccaaccc
     6061 tcaagattac cgtcaaaatc acgtaaacac ccagattgtt taaggttttc gtggagcttg
     6121 tcaatacgcg ggtgaccacc ttccagagga atcatgtaaa cgggtttgcc ggtcgtgcaa
     6181 gcttcggaca acatcgaagc actgtcggcg gtaaccagaa taaaatccgc ccatgccagc
     6241 aggccaaaat acggattttc actttgcccg ttccagaaat aatgattgcc caattctctg
     6301 cgcaaaatcg cttcattctc tgcgccggtg cggcgcgaag cagtaatcat caagctggca
     6361 tcaatgtttt tgagttgcgc cgccaatcgc cgcgtaatct cgctggtcat cccataggct
     6421 ttgctgctgc cgccaattaa caccgccacg cgcggagtcg aaagcttgcc gaactgtgaa
     6481 aattccgctt ttgcggtttc cagctttttc ggtgtaactc ggttgggcgc tgaggtcgtg
     6541 acaatcacat tttccccacg tgtcggatcg tgcgcgggca ccgcaaccag atcaaactgc
     6601 tttggaaaaa tgcgcggatc ctgaatttgc acggtaaatg tttttccgcc gcttgccttt
     6661 ttaatatagc gcgaagctgc aatcgattta cgccccgagg caagcagtaa atctggccat
     6721 ggccccgtaa gggcagggga gaagctccag ttttgctcaa agcccaaata gggcgagagg
     6781 cttttccacg gctgacgaag ctctatgcgt ttaacttccg gcactatgcc cagagcctcg
     6841 gccacgccaa gacattggtt ttccgtgccc gcaaggcctt cggtaacaac ccagcaggta
     6901 atattttttg ccgtattttt tgaaagttct tgcatcatgg cttgcgcaac tttatatcaa
     6961 gctcataggt ctttgtgaaa cgatttaatc taaaaaagaa cccctccatg cgccgttcaa
     7021 aactcgatgc tattgataaa aaaatcctgc aaaatctaca agctgatggc cgaatgacca
     7081 atgtcgaatt ggccaaagtc gtcggtattt cagcgccgcc atgcctgcgc cgtgtgcgcg
     7141 cacttgaaga ggcaggctat attcacagct accacgcgca gattgatccg gcaattctgg
     7201 gctacagcgt cacggtgtat gctatggtgc gccttgagtc acaagccgaa aaagacctcg
     7261 aagcctttga aaaacacatg gttgagctgc cgatggtgcg cgaatgtcat atgctggccg
     7321 gcgatgtcga tttcatcctg aaaattgtgg ctaaagactg ggatagctat caggaatttc
     7381 tgactcatga actgaccaca gcgcccaatg tagtctcggt aaaatcttcg ctcaatatcc
     7441 gcagcgccaa agacgaagct ggcgtcccga ttgagcaata accaggcttt atttatattt
     7501 caccgccaaa atctcgtaat cgcccttacc tttcggtgtt tgaatcacga cagaatcgcc
     7561 ttctgatttt ccaatcagcc cacgcgcaat tggagagcta atggagattt tgctgtgtgc
     7621 taggtcggcc tcataatcac caacgatttg ataggtggac tcttcatccg tttcctcatc
     7681 aactatggtc acggttgcgc cgaacttcac cgcgctaccg gacagggtgg atggatcaat
     7741 cacctgcgct cgcccaatca ccgcttcaag ttctttaatg cgcccttcga taaaactctg
     7801 ctgttcacgc gcggcgtgat attccgcatt ttccgacagg tcgccatgtt cgcgcgctgt
     7861 ggcaatcgcc tcaatcacat tcgggcgctg cacggttttc aaatctttca attcggcctc
     7921 taaaccttca aaaccgattt tggtaacagg aatctgttcc atgtcgttct atcctttttt
     7981 cgtcattgcg agcgtagcga agcaatccaa ctcttatact cttgttctga agatggattg
     8041 ccgcgtcagg ccattcggcc ttcctcgcaa tgacgggtgg ggtcttcaaa ataaaacccg
     8101 ccaaacgcgg cggggattaa cgcgatgcta tgcgaaattt tagttttcgt caagattgat
     8161 gtatagatcc taaaccctat tgggtgtgtg ctcatctata aagcttctaa atgcatcata
     8221 accattatta tatgcatgat gcatggcttt ttgaccgcca ctatatgcat ctgggtcacg
     8281 ccgatctttt gtcttgtcgt aagtagtatc caagactaaa atttttgatt gtccgccagc
     8341 agccatcacg ccttctgtaa acttttcgaa agcgtcgaca gggatgttta caatatcttg
     8401 cttgtcacac gctgttcggt gtgcattggg tatggaatgg tgaataatgg ttgcgttttg
     8461 tccagagtcg ctactgtcaa gatctgcgga aatagcaaat gcggttttaa ttgctccccc
     8521 aaatttataa acaaaaagac tcataacgac gctccttaac tgaatttatt tttcgtaagg
     8581 taataaggat ttaatattat gtcaataaaa gcttacgccg cgtctttttt ggccttggcc
     8641 ccgaccggga aaaactcctg caacggcgcg acctcgatat ccttggcctt gatcgccgca
     8701 atcgctttga ccgccgcgct ggccgcggtg agtagcgtgt aatacggaat cttgttcatc
     8761 aacgccatgc gtcggatcga gctgctgtcg gctacggcct gcgatgattt cgtcgtgctg
     8821 ttaatcacca gcgcaatctc gccattgatc atcgcatcca gcacgtgagg ctggcct
//
LOCUS       DAOSWR010000085         8846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5065_length_8846_cov_5.841770, whole genome shotgun sequence.
ACCESSION   DAOSWR010000085 DAOSWR010000000
VERSION     DAOSWR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_8846_cov_5.841770"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..580
                     /locus_tag="PK513_07105"
     CDS             197..580
                     /locus_tag="PK513_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82254.1"
                     /translation="MRQFLVGFLTLVMLTPSLVCGMADCPMPMQAEKKPCHQSGDMDD
                     DMQGKDGPMLAADCMGVDFFQVDVSNDIQPDLSVDSVDYAWIDLVTDYNFQTDNINGI
                     RGPPGRMLDPRSQPSLILITQRFRI"
     gene            632..1780
                     /locus_tag="PK513_07110"
     CDS             632..1780
                     /locus_tag="PK513_07110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO82255.1"
                     /translation="MRVLFFVLVLFAFVPVAVAAEEYICPMHPHISGEEGNNCPICGM
                     TLVPKVNEMPEGKSHEVHGSAPEGAFHIDPSYVHALGVKTAEVTHHEFGKAIRAFGKI
                     STNTRLEYAIDVRSKGWIVDLPVDAVGDTVKKGDLLFTYYSPDLMSAQSDFLLGSRVG
                     NARQRLRLYGMDDKAIAELTKKGKFLDKTPFHAPVDGTVTMLNVRKGSHVNEGGSVMK
                     LQDFSRLWVNIDVPVRDVQFLAVGTPAQITVPETGEVYETVIDFIHPVNDPKTRTVMV
                     RLILENHDGILKLDTYVDAVFNADVQSRLAVPAEAILYGSMGAYVMENVKDGYFKPVM
                     VKTGITANGLTEIKSGLLHGQRIVTSGQFMLDAESNLKGGMEAMGHVH"
     gene            1770..5006
                     /locus_tag="PK513_07115"
     CDS             1770..5006
                     /locus_tag="PK513_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013301489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOO82256.1"
                     /translation="MSTDQNNLLWHDPSKSFVAKIIDWSVGNRFFVMLGALVLLAVGV
                     VSITKIPLDAIPDLSDTQVIIRTEFPGQAPQVIEDQVTFPLSTQMLGLPEAKVVRGFS
                     MFGTSFVYIVFEDDVDMYWARSRVLESLSQVRSELPEGADPQLGPDATGVGWVFQYAL
                     FDRTGQHDLSELRSLQDWFVRFELATIDGVAEVASVGGFVKEYQVLVDPNKLRAFDIS
                     LAMVIEAVRSANQETGGRTLEMAETEYLIRSFGYVNKPEDLQQAVLRAINGTSITVGD
                     VARVVEGPAIRRGVTELNGVGEAVGGIVVMRPGANALDVIHSVKERLESVKQGLPPGV
                     EIKIVYDRSALIEGSVDYLKDKLIEESLVVALITFLFLLHARSAFVAIITIPLGILAS
                     FIIMNAQGITANIMSLGGIAIAIGAMVDASIVMVENAHRRLSSLRAQRSNPEIDCRIS
                     DAPRNDEKENAEERKKAILQAAKEVGPGLFFSLLIITVSFFPVFALTGQSAKMFAPLA
                     FTKTYAMAAAAFLSVTVVPVLMLWLVRGKIRREDENPLNRFFIAIYRPFLNLALKWRK
                     TTIVLAIAALVSTAFPASKLGREFMPPLYEGGLLYMPMTLPGVSVTKAREILQVTNRQ
                     IMSVPEVEFAFGKAGRADTATDPAPLAMIETWIELKPREEWRKGMTPQKLIDDLNKRV
                     GLPGMMDSWGYPIKIRLDMLTTGIRTPIGIKVAGSDLTTIDKIAKDVEAIAQNVSGTR
                     SAIADRVVGGKYIEIEPDRREIARYGIALGTVQRIIQTALGGVQLDEAVEGRERYPIM
                     LRYDRPFREHISELDNILVPTPDGAHIPLATLADIRIAEGPAMIRSEDARLNGWVFVD
                     IEGRDIGSYITDLQDALKSLDLPQGYTLKLSGQFEQMQEAGERLKMAVPLTLLIILTL
                     LYLHFRTWDRTLLIMLSVPFGIIGGVWWIWLAGYNVSVAVAVGFIALAGIAVETAIIM
                     LIYLDQQMREHPVRSFEDLLENVRHGAIQRLRPKLMTVSTIILGLIPIFLTTGPGSDV
                     MRRIALPMASGMVSTLVLTLILIPVLYTVYMQWKMKLPVKEDEAERNE"
     gene            complement(5287..5361)
                     /locus_tag="PK513_07120"
     tRNA            complement(5287..5361)
                     /locus_tag="PK513_07120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5327..5329),aa:Thr,seq:cgt)
     gene            complement(5431..6276)
                     /locus_tag="PK513_07125"
     CDS             complement(5431..6276)
                     /locus_tag="PK513_07125"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOO82257.1"
                     /translation="MFPTATIVFREIIEVALVVSIVLAATRGMPSRIHLVLAGLGVGV
                     FGSVVIAFFTDAISQAIDGIGQEIFNAGVMFIAVGFLSWTVIWMKTHGRQLAQNIKAV
                     GADVMAGDKSAYVLVGVVALATFREGAEIALFTYGMTASGAFPLSAIVMGGILGTIGG
                     AAIGLMLYMGLLKAAQKHLFSITGWMLIVLTAGMAAQGANFLIAAGVLPPLHPEVWDT
                     SALISGQSFIGETLRVLVGYTPRPTGMELVFYGTVLLGVGTAYKRVGKPVSSHRKALP
                     LVAAE"
     gene            complement(6320..6628)
                     /locus_tag="PK513_07130"
     CDS             complement(6320..6628)
                     /locus_tag="PK513_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOO82258.1"
                     /translation="MATLDGKIVQSKTNADGAYELVIKDHMFTPDELHVPAGQKIKLV
                     VHNQDPTPEEFESDDFRREKIVPGNSSATIYVGPLKPGKYHFFGEFNLDKANGYLYAE
                     "
     gene            7524..8255
                     /locus_tag="PK513_07135"
     CDS             7524..8255
                     /locus_tag="PK513_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO82259.1"
                     /translation="MKAFLVIIAQCIQNFGTAYGLPVSLFLAGLFGGLTHCAGMCAPF
                     VLAQTTQETRLQKLGSRLLLPYHLGRMTTYVTLAVIVNALINMAFLFSDLKILITAPL
                     LMLAGTIFIITAFPNASALFPWAARLQIVPTFGIISRLGKNFMTDPNLLKRYGLGVLL
                     GFMPCGLVLSALLASATADTPLYAAFAMSAFTLGTIPALLMVTLGGQALKKAYPKATQ
                     RFSRGAMIISGLWLFTLAGIMLTGD"
     gene            8277..>8846
                     /locus_tag="PK513_07140"
     CDS             8277..>8846
                     /locus_tag="PK513_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOO82260.1"
                     /translation="MTATTLSEKPDYNDDVVKLFTLATIFWGVTGFLVGVLIASQMVF
                     PDLNIEPWLNFGRLRPLHTSAVIFAFGGNALFATSYYVVQRTCGVRLWSDKLANFTFW
                     GYQAVIVMAGLGYVLGVTQGKEYAEPEWYVDLWLTVVWVAYLLVYLGTVIKRKEPHLY
                     VANWFYLAFIITVAVLHLGNNIQIPVELLG"
BASE COUNT         2190 a         2063 c         2158 g         2435 t
ORIGIN      
        1 tctcatgatg aaatctcctc tcatcattag tgaatcatga attatcagat tagggaatcc
       61 tgtttatagg tcctgaacct aggcttaaac attttctcct gacggcggac agccgcatgc
      121 tacatacgat tttaagagaa ctttgttttt gagaaacttt aggatttttt tacgcgaata
      181 gtgtaatatg aagcttatga gacaattttt ggttggattt ctgactcttg tcatgctcac
      241 gccttcgctg gtgtgcggta tggctgattg tccgatgccg atgcaggccg agaaaaagcc
      301 ctgccatcaa tccggggata tggatgatga tatgcagggc aaggatggcc ctatgctggc
      361 ggcagattgt atgggcgttg acttctttca ggtggatgtc agcaatgata ttcagcccga
      421 cctgtctgtt gatagcgttg attacgcttg gattgatctg gttactgatt acaatttcca
      481 gacagacaat attaatggca ttcgcggacc gcccggcagg atgctcgatc cgcgaagcca
      541 gccttccctc atcctcataa cgcagcggtt tcgcatctaa atacgctctt ttctcgacgg
      601 tttttcacgc ctcaaaaaag gagcagttct catgcgtgtt ttattttttg ttctagtgtt
      661 atttgccttt gtgcctgtgg cggttgcggc agaggaatat atatgcccga tgcatccgca
      721 tatcagcggt gaagaaggta ataattgccc gatttgcggc atgactttag tgccgaaagt
      781 taatgaaatg ccggaaggca aaagccatga ggtacatggt agtgctcccg aaggggcgtt
      841 tcatattgat ccgtcctatg ttcacgctct gggcgttaaa accgctgaag taacacatca
      901 tgaatttggc aaggccattc gcgcttttgg caagataagt accaataccc gccttgaata
      961 tgcaatcgat gtgcgttcaa agggctggat tgttgatctg cctgtggatg ccgtaggcga
     1021 tacggttaag aaaggcgact tgctgttcac ttattacagc cctgatctta tgagcgcaca
     1081 gtcagacttt ttactgggta gccgggttgg aaacgccagg caacgtctgc gtctttacgg
     1141 catggatgac aaggcgattg ccgaattgac caagaagggg aagtttctgg acaaaacgcc
     1201 ctttcatgcg cctgtcgatg gaacggtaac gatgctcaat gttcgcaaag gatcgcatgt
     1261 gaatgaaggc ggcagtgtta tgaagttgca ggatttttcc aggctctggg ttaatatcga
     1321 tgtgccagta cgcgatgttc agtttttggc tgttggcacg cctgcccaaa tcacggtgcc
     1381 agaaacaggt gaagtctatg aaacggtcat tgatttcatt catccggtga acgaccccaa
     1441 aacccgcacg gtgatggtgc gcctgattct cgaaaatcat gatggaatat tgaaactaga
     1501 cacctatgtt gatgctgttt ttaatgctga tgtgcaatcg cgtttggcag tgccagcgga
     1561 ggctatcctg tatggcagca tgggcgccta tgtcatggaa aatgtaaagg acgggtattt
     1621 caagcctgtc atggtcaaaa cagggatcac ggcaaacggc ctgacggaaa tcaaaagcgg
     1681 ccttttacac ggccagcgga ttgtaacttc ggggcagttc atgctggatg ctgaaagtaa
     1741 tctcaaaggc ggtatggagg ctatgggcca tgtccactga tcaaaataat ctcctttggc
     1801 acgatccatc caaaagcttt gtagccaaga tcatagactg gtcggtggga aaccgcttct
     1861 ttgtgatgct cggtgccttg gtgttattgg ctgtgggggt tgtttccatc actaaaattc
     1921 ctctggatgc tattcctgac ctgtcggata cgcaagtgat tattcgtacg gagtttcccg
     1981 gtcaggctcc gcaagtgatt gaagaccagg tcacttttcc actgtccacg caaatgctgg
     2041 ggctgcctga ggctaaagtg gtgcgcggct tttccatgtt cggcacatct ttcgtttata
     2101 tcgtgtttga agatgatgtt gatatgtatt gggcgcgcag ccgcgttcta gagtctttgt
     2161 cacaggtaag gagtgaattg cccgaagggg ctgacccgca attaggtcct gatgcaacgg
     2221 gtgtgggttg ggtgttccaa tacgctttgt ttgaccgcac ggggcagcat gatctttcgg
     2281 aactgcgcag tctgcaggat tggtttgtgc gttttgagct ggccacgatc gatggcgtgg
     2341 cggaagtcgc cagcgttggc ggatttgtaa aggaatatca ggttctggtt gatccgaata
     2401 aactgcgcgc ctttgacatt tctttagcaa tggtgattga ggccgtgcgc agcgccaatc
     2461 aggaaaccgg tggacggaca ctggagatgg cggagacgga atatctgatc cgctcttttg
     2521 gttacgtgaa taaacccgaa gatttacaac aagctgtatt gagagccata aatggtacgt
     2581 ctattacggt tggcgatgtt gcgcgtgtgg tggaaggtcc ggccattcgg cgcggtgtca
     2641 cggaactcaa cggcgtgggt gaagccgtgg gcgggattgt ggttatgcgc cccggtgcca
     2701 atgcgctgga tgttattcat agtgtcaaag aacgtcttga aagcgtgaag cagggtttgc
     2761 cgccgggcgt ggaaatcaaa atcgtttatg atcgcagcgc gctcatcgaa ggctcggtgg
     2821 attatctcaa agataagttg attgaagaaa gcctggtggt ggcactcatc actttcctgt
     2881 ttttgcttca tgcgcgaagt gcttttgttg cgattataac cattcctctt ggaattttag
     2941 cctctttcat catcatgaac gcgcaaggca ttaccgccaa tatcatgtcg ctgggcggta
     3001 tcgccattgc catcggtgcg atggtggatg cgtccattgt tatggttgag aatgcgcacc
     3061 gccgtttatc gtcattgcga gcgcagcgaa gcaatccaga aatagattgc cgcatctctg
     3121 acgctcctcg caatgacgaa aaagagaacg ccgaagaaag aaaaaaagct atcctgcaag
     3181 cagccaagga agttgggccg ggactgtttt tctcgttgct tattatcacg gtatccttct
     3241 ttcctgtttt tgcgcttaca ggccaatcag ccaaaatgtt tgcgccgttg gcttttacca
     3301 aaacttatgc gatggcagcc gcagcgtttc tgtctgtgac tgttgttcct gtgctgatgc
     3361 tttggctggt gcgcgggaaa atcaggcgtg aagatgagaa cccactcaat cgtttcttta
     3421 ttgcgatcta ccgtccgttt ttgaacttgg ctcttaaatg gcgaaaaacg accattgttc
     3481 tggctatagc cgcattggta agtacggcct ttcctgcttc aaaattgggg agagagttta
     3541 tgccgccgct ttatgaaggc gggctgcttt atatgcctat gactttgccc ggtgtctctg
     3601 tgactaaagc cagggagata ttacaggtga caaaccgcca gatcatgagc gtgccggaag
     3661 tagaatttgc ctttggtaag gccggccgcg ctgacactgc aactgatcct gcacctctgg
     3721 ccatgattga aacgtggatt gaactcaagc cccgcgaaga atggcgcaag ggaatgacgc
     3781 cgcaaaagct gattgatgat cttaataaac gcgttggttt gccggggatg atggatagtt
     3841 ggggctatcc gatcaaaatc aggttggaca tgctaaccac gggtattcgt acgcctatcg
     3901 gcataaaagt tgctggttca gacctcacga cgattgataa gattgccaaa gacgtagaag
     3961 ccattgcgca gaatgtctca ggaacacgca gcgcgattgc tgatcgtgtc gttggtggca
     4021 aatacattga aattgaacct gatcgccgtg aaatagcacg ttatggtata gcgctaggaa
     4081 ctgttcaacg cattattcaa acagcccttg gcggggtgca gctagacgag gctgtggagg
     4141 ggcgtgagcg ttaccctatc atgctccgct atgaccgccc attccgcgaa catatttctg
     4201 aattggataa tattttagta cccacgccag acggcgcgca tattccactg gcgaccctcg
     4261 ccgatattcg catagctgag gggccagcta tgatcagatc agaagatgcg cggctgaacg
     4321 gctgggtgtt tgttgatatt gaaggacgtg atattggctc ttacatcacc gatttacaag
     4381 atgcactcaa atcactcgat ttgccgcaag gctatacgtt gaaactctcc ggccagtttg
     4441 agcagatgca ggaagcgggt gaacgcttaa agatggctgt tcctttaaca cttctgatca
     4501 tcctgaccct actgtacctg catttcagaa catgggatcg aacgcttttg atcatgctat
     4561 ctgtgccgtt tggcattatc ggtggtgtgt ggtggatatg gcttgcagga tataacgtct
     4621 ctgtggccgt tgctgttggc tttattgcac tggcaggtat cgctgtagaa acagccatta
     4681 tcatgctgat ttatctggat cagcaaatgc gtgagcatcc cgtacgtagt tttgaggact
     4741 tgttagaaaa tgttcgtcac ggtgcgatcc agcggttacg tcccaagctt atgacagtca
     4801 gcaccatcat tcttggtctt attccgatct tcctgaccac tgggccgggt tcggatgtga
     4861 tgcgccgtat tgctctgcct atggcctccg gcatggttag cacgcttgta ttaacgctga
     4921 tccttatccc tgttctctac actgtgtaca tgcaatggaa aatgaagctg cctgtgaaag
     4981 aagatgaggc tgagcgcaat gaatagcaag gaaaaatttt aaaactcgaa atgctggcgt
     5041 tgttcattcc agaggatggg aaggtcgttc gttatctcag ccagctgctg tgcagctggc
     5101 ggccatcgat aatcgcggta gtgatgtcgg gagcgtgttc tgtcaggcgg atgattttgc
     5161 aggcatagcc gctgtccaac ttgtgctgac gttatacagt cttatagttg caaagatggc
     5221 tttttgaaca caaaaagtcc tttcctttca acgctatagg gagcttttac gggcaggaaa
     5281 acaaaatggt gccgctgggg tgatttgaac acccgacctt agctttacga aagctctgct
     5341 ctacccctga gctacagcgg ctttggaaga tatgttttaa aatcttttgt gatcgcttgt
     5401 caatctgcta cttggaccaa gcaggcaaaa ctactccgca gcgacaagag gtagagcttt
     5461 acgatgagaa gaaacgggtt ttccaacgcg cttataggct gtgccgacac ccaaaagcac
     5521 cgttccataa aacaccagtt ccatacccgt aggacgcggc gtatagccta ctaaaaccct
     5581 caaggtctca ccgataaaac tctgtccgga aattagcgcg gaagtatccc acacttccgg
     5641 gtgcagcggc ggcaaaacgc cggccgcgat gaggaaattt gctccttgtg cagccatgcc
     5701 ggcagtaaga acaatcagca tccaacccgt tatgctaaac agatgttttt gcgcggcttt
     5761 caacaaaccc atatagagca tcaagccgat tgcagcgcct ccaatggtcc ctaagatgcc
     5821 gcccatcaca attgcagata acgggaacgc ccccgaagcc gtcatgccat aggtaaatag
     5881 cgcaatttca gcgccctcac gaaacgtggc cagcgctaca acaccgacaa gcacataggc
     5941 cgacttatcg cccgccatca cgtcagcccc cacagccttg atattctgcg cgagctgacg
     6001 cccgtgggtt ttcatccaga ttacagtcca gcttaaaaac ccaactgcga taaacattac
     6061 gcctgcatta aagatctcct ggcctatgcc gtcaattgct tgcgaaatgg catcggtaaa
     6121 aaacgcgata accacagagc caaaaacgcc aacgcccaga ccggccagta ccagatgaat
     6181 gcgactcggc atcccccggg ttgccgctag cacaatgctg acaacaagcg ccacttcaat
     6241 aatttcacga aaaacaattg tagcagtggg aaacatggct ttagatcctt tattcctgat
     6301 ttatcgcgtt tctttaaaac tattcggcgt acaaataccc attggcttta tcaaggttaa
     6361 actcgccgaa gaaatggtat ttaccgggct tgagcgggcc gacataaatt gtcgcactgg
     6421 aattgcccgg aacaattttc tcacgccgga aatcatcact ttcaaattct tccggtgttg
     6481 ggtcttggtt atgtactaca agtttaatct tttgaccagc cggaacatgc aactcatcgg
     6541 gcgtaaacat atggtctttg atcacaagct catacgcacc atccgcattg gttttgcttt
     6601 gcacaatctt accgtctaaa gtcgccacag ccgcttctag tttggcaaac cattcacgca
     6661 cttgtgcgtc ttcgtgattt tcagaagcat aatgcactgc ttgcaccgct tcatctatag
     6721 cgcctaattg atcggcttta ccagcctttt cggcaatcat cttgtcgatg gcagtttcca
     6781 gagaatagct aatctcatgg attctttcca gatcactgtt ggaaagcatc tcagctttta
     6841 tcaattcggc aatctctttg ttatgagctt tcaacgcttc aagcgcctca tcagttgttg
     6901 catagctctg tccttcgtag tgcatggtgc gctcttcatg cgtaacgtta tgctgcacat
     6961 cttcggccac agccacagaa gaaacaccac tagccaaacc aatcatagcc atagacaaca
     7021 acaatttttt catatcagac attccattta cgttaagaaa actaaacttg agaactattc
     7081 tcaaaagtta tgcaatactg ctcactttat tcacgattta cgccctttca actcaaatgt
     7141 aaatgataat tattctcaaa atgaaaattg atacgcacaa atcagcccgt ctatttattt
     7201 ttagataaga agtattttta tagttgaagg aaaagaccaa atacaacgcc atttataggc
     7261 acgaaaaaat aacacctgcc ccttttcgta agtaatttta cgtgaaattg tgccaagtgt
     7321 ttttataaaa ttgttatcgc aacacttaaa tgaagggtgt gagtaaatgt ccaccttatc
     7381 tggttgagaa gattgagcgt ttaaatttct atgcataaga ttttttactt ttcccaatgc
     7441 tatattttaa aaatatggac ttgaaaatta gcgataatca aggcaataat ctaatgcgtt
     7501 tttttaaata atgaaaaagc aacttgaaag cctttcttgt gatcatcgcg caatgtatcc
     7561 agaatttcgg cacagcttac ggcctccccg taagcttatt tctcgccgga ctattcggcg
     7621 gacttacgca ttgtgccgga atgtgcgccc cctttgtact ggcccaaacc acacaagaaa
     7681 cccgccttca aaaactaggc tcaagacttc tgcttcccta ccatcttggc cgcatgacca
     7741 catacgtaac cttggcggtg atcgtaaatg cactcattaa catggcgttc ctgttttccg
     7801 atttaaaaat tctcattaca gctccgcttc ttatgctggc aggcacaatt tttatcatca
     7861 cggcatttcc aaatgcgagc gccctttttc cttgggctgc ccgcctgcaa atcgtcccca
     7921 catttggaat catctcccgc ttgggcaaaa attttatgac cgatccaaac ctgctaaagc
     7981 gttatggctt gggtgtttta ttaggtttca tgccctgcgg ccttgtttta tcggcccttc
     8041 tggcctctgc aacagcagat acaccacttt acgcagcctt tgcaatgagc gcctttactc
     8101 tgggaacaat tccagcctta ctcatggtaa ccttgggggg gcaagcattg aagaaggcgt
     8161 accctaaagc aacgcaaaga ttttcccggg gagcaatgat aataagtggc ctttggctct
     8221 ttactttggc cggcataatg cttacaggag actaggtttt taaaaaggac gaaaaaatga
     8281 ctgcaaccac actttccgaa aaaccagact ataacgatga cgttgttaag ctcttcacgc
     8341 ttgccaccat attctggggc gttacgggct tccttgtcgg cgtacttatc gcttcacaaa
     8401 tggttttccc tgacctgaat attgagccat ggctcaattt tggccgcttg cgtcctttgc
     8461 atacatccgc agtgatcttt gcttttggcg gaaatgcctt gtttgcaacc agctactacg
     8521 ttgttcagcg cacttgcggt gtaagacttt ggagcgacaa actggcaaat ttcactttct
     8581 ggggttacca ggctgtcatt gttatggccg gtttgggtta tgttctgggc gttacgcaag
     8641 gtaaagaata tgcagaaccg gaatggtatg tggacctatg gctgactgtt gtctgggttg
     8701 cttaccttct ggtttatctg ggaacagtaa tcaaacgtaa agaaccgcac ctctatgttg
     8761 cgaactggtt ttatctggct tttattatca cggtggccgt cctgcatctt ggtaacaaca
     8821 ttcagattcc cgttgagctt ctcggt
//
LOCUS       DAOSWR010000086         8727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5156_length_8727_cov_5.117043, whole genome shotgun sequence.
ACCESSION   DAOSWR010000086 DAOSWR010000000
VERSION     DAOSWR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_8727_cov_5.117043"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /gene="lepA"
                     /locus_tag="PK513_07145"
     CDS             complement(<1..1338)
                     /gene="lepA"
                     /locus_tag="PK513_07145"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOO82261.1"
                     /translation="MSTHETKPLEHIRNFAIIAHIDHGKSTLADRLIQTCGGLEEREM
                     QEQVLDNTDIERERGITIKAQTVRLEYIAKNGIAYQLNLMDTPGHVDFSYEVSRSLAS
                     CEGSLLVVDASQGVEAQTLANVYQALDNDHEIVPVINKIDLPAACPDRVKTEVEDVIG
                     LDCSEAIEVSAKTGKGIDALLEAIVKVLPPPHEGSADKPTKALLVDSWYDIYLGVVVL
                     VRVVDGYLRKGQKIKFMSNGRVREIDRVGMFTPKKVELDVLGPGEMGFITASIKDISE
                     TNVGDTITDEKRPCAERLPGFKPSIPMVFCSFYPADAGDFEKLRESLGKVKLNDASLH
                     YEPESSQALGLGFRCGFLGLLHMEIIQERLEREFDLDLIPTAPSVVYKLNLTNGEKME
                     LYNPVDMPEVQRIETIEEPWIKATILVPDEYLGGLLQLCQERRGVQLDLTYAGG"
     gene            complement(1384..1881)
                     /locus_tag="PK513_07150"
     CDS             complement(1384..1881)
                     /locus_tag="PK513_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82262.1"
                     /translation="MAKKRFELGDLTNEMRLSINGLPPAQHKFTKTLVKNVVLNVIDV
                     FTSFDERNRFTGVVAFVPTYNTEEAKKSLREGDVISVRTFLPIEKLHCLSETDNTSLT
                     LKEIEVNTWGDGVITCYDTTPKDFTPGTDENNERAMDAIRAMSQGHNYTDEDLNGPGS
                     DSTPS"
     gene            2012..2503
                     /locus_tag="PK513_07155"
     CDS             2012..2503
                     /locus_tag="PK513_07155"
                     /inference="COORDINATES: protein motif:HMM:NF023148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HOO82263.1"
                     /translation="MDEERKQPEKKIILQDRTAPSPSSPALWLALLAVPLVFGLIVGL
                     GQKEGRQRLPDTLEEMIPDVPVSNRGRGGTTVAPGRDPVIGAAIKTATPPELKSYTCN
                     FEPWIGLKLSQEMLDALKGASTGERPYRILRPGDAKTQDYSPTRVNFEIDKAGTITRV
                     WCG"
     gene            complement(2506..2655)
                     /locus_tag="PK513_07160"
     CDS             complement(2506..2655)
                     /locus_tag="PK513_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82264.1"
                     /translation="MSVIDMKTIASDSFGIDFQGTFRTACFGGQQLDSCAAPVVTNAM
                     DLTNG"
     gene            complement(2726..3094)
                     /locus_tag="PK513_07165"
     CDS             complement(2726..3094)
                     /locus_tag="PK513_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82265.1"
                     /translation="MTEDTHNEISDRPSVGNLTDFVYGVNEDNRLDIEVAIKEDGRVV
                     VFHSHPFKNDIAWFEFDLDTNKLDFVMDDGDIRDIGLPLSQSVAVHMQNSHQILMVLL
                     DPETGEAKEGNYIPLIIHRN"
     gene            complement(3249..3617)
                     /locus_tag="PK513_07170"
     CDS             complement(3249..3617)
                     /locus_tag="PK513_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82266.1"
                     /translation="MTDKPEHKKDSIKKAFTPGEAKDKPSLFLPDEMDIYCNKITKET
                     FIFHGKAIDYDALERMEYDPKTHEVDVFLKDGSVLDLGVKIQWLIRPYFTKAEEVQIV
                     QTKDGEAIDGTFVPITHKGK"
     gene            complement(3750..8681)
                     /locus_tag="PK513_07175"
     CDS             complement(3750..8681)
                     /locus_tag="PK513_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82267.1"
                     /translation="MSDVQTRIADGISTYSDLNNRTADQNVAMLYMMNVIDHKNTSMS
                     GVWRGIKSMATDYVSWGIAGASFVTGFFSGGAGWVAGGTALAARAAALAPKMAGSAFG
                     LALKNKVTQQITTKVANTAIVQNIAQHKATQFIANHAVTKALTSEAAVTAGQAAITGA
                     AVESLLSDRYLTYEIENKGHQALGDNNYEYSLGRGVNALVMGGTFGWVLGRGGGALGH
                     SLGKKFKKNADGVADEAAAPEAEAKTEAKTEAKTEAKAEAETLKTDTPKVDEPVVEPV
                     KTDEPVATSPEADNIATEPPEVEFPDTKAENEEKYSEYFPTSGPKDRVSPKDVVDHLE
                     RVKNNSSRDVNQDDHVEVTLTGTREQVNKVIDRLGGGSGGAPAETAGTTRAAEVETGA
                     PAPEAGAKPGKAADDGKATERVEAVEPEAVTKKPEATTAGADEVPLTHTPPKKANNKA
                     NLIDAPERSWIHKVIHWFPVTRIGAWIPGSVNPAGHLPQELSFKRYINSYINTVDDLI
                     LGKSAGEGAGGANQRTAQNIAKRTKSVIGDNGTLKDLDVIFNKLDEDLKNIASRVDVG
                     GPESLTKKQAQEMAIDVVENFTTNHKVALKEFNDALSGITAEMKLESQLAEKIDLLKF
                     GQQKSSDVKPDPGKLADEAEVLIDRYEQTTGRDFIGKEERIQFIRNGAQLRGSDKKWE
                     QWRIDRMDGKNQYFYLPSYQANAMFQDAKGLQATVHSLLDDNFATGEYAKELAEGLKH
                     PETDVQSHAFTNTVDLLINQTRGARMRRENRDASQNLFWFMRGAAKPDTDLIEINRKV
                     NVLSIGYDNPYKRTYHETLPGGQPNNMEWSIMDVEKMDPADANLADKLLGISDTAYTQ
                     GNEHLFLEAFKRLNQRSIKKGSDGVVSGERVLIKAFISKLNGMRALRKAAGPHYEHYE
                     KAIRALEDATHSPYEYAQREVHSMYARGSYIPYHARKFVGRGSAISTPGQMYVTAWRS
                     KATQAYIKSRLFGGQIKQQKIDEENSIFYPTWKYAGLVGEDAKGWAKWSARLSRIPGY
                     NLATDPLRLGYHTTKYLMTNRFAHEMAINVGYFGLQAWAVEEGLEAAGSYFIPEYWND
                     TWITDEEGNIDILGFRPDIANRFAEYAVMWPVEAALTPARWTFSAYDWAIEKALGKEN
                     DGLFGTGLESKHLSLYQDLPYTKDLFGKKHSLASGFVTLLGDRWDDVENHSTPSDAST
                     PEAKDQQQAIDVPPDVDAAFIRNTLDPNIAKRKTEVEIAESALKTAQRDHARLKKIAG
                     KDDSDVNITARDDAETAKKNAQTALERHETTLEALQFERTKAVIAFRASKFKDDPGLQ
                     SKITAILWDMQDAGPKISKKFDLLDDALFTGALDLAGKEGEYTKKDLQGAITKVFPAD
                     KKSRKNDQENQPYSQGGTRPSESFGKMAEDGVNWALDWTGADAKSGWLGRTFNKLGDW
                     GRAFGGFWGDLSTPWKAGIIGVAGFFGIGKFLKKQLGAFGNLPFVGLILGLIGFALTG
                     KAAHAYMNRPKKGSVQGTNGFNPNPTEDSQKHGEKKATMGGVKTTGKFTVMKGQKVTD
                     TREIETGMYAVQANTSGKKGFAPRNVVYLMTNDGIGDSALEDQAKTARHIPVGLKNVI
                     SAKDSAFDGITHDNRALRRTSANDFEYDAPAVA"
BASE COUNT         1981 a         2334 c         2019 g         2393 t
ORIGIN      
        1 ccccccggcg taagtcaggt caagctgcac cccgcgccgc tcctgacaca gttgtaacaa
       61 tccccccaga tattcatcgg gcaccaaaat ggttgccttg atccatggtt cctcaatcgt
      121 ttcaatccgc tgcacctctg gcatatcaac cggattatac agctccatct tctcgccatt
      181 ggtcaggttc agcttataca ccacgcttgg cgcggtcggg atcagatcaa gatcaaattc
      241 ccgctccagc cgctcctgaa taatctccat atggagcagg ccgagaaagc cgcagcgaaa
      301 accaaggccg agcgcttggc tgctctccgg ctcataatgc aggcttgcgt cattgagttt
      361 tactttgcct aaggactcgc gcagcttctc aaaatccccc gcatcggccg gatagaacga
      421 acaaaacacc atcgggatcg acggtttaaa gccgggcaaa cgctctgcgc aagggcgctt
      481 ctcatcggtg attgtgtcgc cgacattggt ttccgaaata tccttgatcg aggccgtgat
      541 gaaccccatc tcgccgggcc ccagcacgtc taactccacc tttttcgggg tgaacatccc
      601 aacgcggtca atctcacgca cccgcccatt ggacataaat ttgatctttt ggcccttgcg
      661 aagatatccg tccacgacgc gcaccagcac caccacgccc aggtaaatgt cataccagct
      721 atcgaccagc aaagccttgg tcggcttgtc cgcactcccc tcatgcggcg gcggcaggac
      781 cttgacgatc gcttcgagta gcgcatcaat gcctttcccc gtcttggcgc taacctcaat
      841 tgcctctgag caatccagtc cgatgacatc ctcaacctcg gtcttaaccc ggtccgggca
      901 ggccgctgga aggtcgattt tgttaatgac cggcacaatc tcatgatcgt tatccagcgc
      961 ctgatagaca ttggccagcg tctgtgcttc cacgccttgg gacgcatcca cgaccagcaa
     1021 agagccttcg cacgacgcca gcgagcgcga aacctcataa gaaaaatcta catgtcccgg
     1081 cgtatccatc agattaagct gataggcaat cccgttcttg gcgatgtatt cgaggcgcac
     1141 cgtctgggct ttaatggtaa tcccgcgctc gcgctcaata tcggtgttat ccagcacctg
     1201 ttcttgcatt tcgcgctctt ccagcccccc gcaggtctgg atcaaccggt ccgccagcgt
     1261 cgatttccca tgatcaatat gggcgataat cgcgaagtta cggatatgtt cgagtggttt
     1321 ggtctcatgt gtgctcatgg acaaaaggaa taggcgaggg aacagcgaaa agcaagcgtg
     1381 gatttatgaa ggcgtggaat ctgatcccgg accgttaagg tcttcatcgg tataattatg
     1441 accttgagac attgcacgta tcgcatccat ggcgcgttca ttattttcat ctgtgcccgg
     1501 tgtaaagtct tttggagtgg tatcatagca tgtgataacg ccgtcccccc aggtgttaac
     1561 ctctatttcc tttaacgtta ggcttgtgtt atcggtttca gacaaacaat gtagtttttc
     1621 aatcggcagg aacgttcgaa cagagatgac atctccttct cgaagcgatt tttttgcttc
     1681 ttcagtatta taggttggaa caaatgccac aacgccagta aagcgatttc gctcatcaaa
     1741 gctcgtgaag acatcaatca catttaaaac aacatttttt accagtgttt ttgtaaattt
     1801 gtgctgggcc ggtggcaggc cgtttatgct gaggcgcatc tcattcgtca gatcgcctag
     1861 ttcgaagcgt tttttagcca taaatatata cccttatctc cgcgaaataa aattatggga
     1921 attatagcat tttcatcttt acgtcaaggg aaacatgaag gaaagataaa gtatgcttga
     1981 gtgaaaagtg caaaacgctt aacgtaaaac aatggacgaa gaacggaagc agcctgagaa
     2041 aaagattatc ctgcaagatc ggaccgcacc cagcccctcc agcccggcgc tttggctggc
     2101 cttgttggct gtgccgctgg tcttcggcct tattgtcggg ctagggcaaa aggaaggcag
     2161 acagcgcctg cccgatacgc tggaggaaat gatccccgat gtaccggtca gtaaccgtgg
     2221 ccggggaggt acgacagttg cgccagggag agatcctgtt attggcgcag cgataaagac
     2281 tgcgaccccg ccggaattaa aaagctatac atgtaacttt gaaccctgga tagggttgaa
     2341 actgtcgcaa gagatgttgg atgcattaaa gggcgcctca acgggtgagc gcccttaccg
     2401 tattttacgc cccggtgatg ctaagacaca ggactacagc cccacccggg tgaattttga
     2461 aattgataaa gcgggtacaa tcacccgcgt gtggtgcggt tgatcttaac cgttggtgag
     2521 atccatggca ttggtcacca caggggcagc gcatgaatca agttgttgcc caccaaagca
     2581 agcggtcctg aaggtcccct gaaaatctat gccgaagctg tcgctggcaa ttgttttcat
     2641 atcgataact gacatgtcac actcctctaa ttatctatta tgttaatgtt tttttattaa
     2701 ttttttatta atgacgcaga cgtttttagt ttcgatgaat aatcaacggg atataatttc
     2761 cttcctttgc ttctccggtt tctgggtcca acaaaaccat cagaatctgg tgagaattct
     2821 gcatatggac cgcaacgctc tgtgacagag gaaggccaat atcgcgtata tcaccgtcat
     2881 ccatgacaaa atccagtttg tttgtatcta ggtcaaattc aaaccaggct atgtcatttt
     2941 taaaaggatg actgtgaaaa acaacaacac gaccatcctc tttgatagca acttcaatat
     3001 ccagacggtt atcttcattt acaccataaa caaagtccgt caggttaccg acgctcgggc
     3061 gatctgaaat ttcattatgg gtatcttctg tcataacagc agtataacca catttttatt
     3121 gaaagaccac atctgcctgt gtccgccttt aacaaatata ttgtaccatg acatttttca
     3181 aaaaatcaaa gaaaatatct atatttttca gtaatttaca tgcgaacttt aaggtgaata
     3241 ttaacctttt atttaccttt atgtgtgatg ggtacgaatg tcccgtcaat cgcctcgccg
     3301 tctttggttt gaacaatctg cacttcctcg gctttggtaa aataaggccg gattagccac
     3361 tggattttca cgcccaggtc caaaacgctg ccatccttta agaaaacatc aacctcgtgg
     3421 gtcttggggt catattccat gcgctctaaa gcgtcgtaat caatggcctt tccatgaaag
     3481 ataaatgttt ctttggtaat tttgttacag taaatatcca tctcgtccgg caggaacaaa
     3541 gacggcttgt ccttggcttc accgggcgtg aaggccttct tgatactgtc ttttttatgc
     3601 tcaggtttgt cggtcataat ttgaatgtgc ccggaaaaaa gggcgaaagg atggatgcat
     3661 agaaaccggg cgcatatgca ccccaacaca atatcatagc atctagacaa gggcggataa
     3721 agggcttgat aagctttgcc tgtaagagct taagcaaccg ccggcgcgtc atattcgaaa
     3781 tcgttggcac tggtgcggcg cagcgctctg ttgtcatgcg tgataccgtc aaacgcgctg
     3841 tccttggcgg agatcacatt tttcagcccg accggaatat ggcgcgccgt cttcgcctga
     3901 tcttccaggg cactatcgcc aatgccgtca ttggtcatta gatagacgac attccggggc
     3961 gcaaagcctt ttttgccgga agtattagcc tgcacggcat acataccggt ctcgatttca
     4021 cgcgtatccg taactttttg acctttcatg actgtaaact taccagtggt cttaacgcct
     4081 cccatcgtgg cttttttctc gccatgtttc tggctgtctt cggttggatt ggggttaaaa
     4141 ccgttcgtgc cctgcacgct gccttttttc gggcggttca tgtaggcatg cgctgcttta
     4201 ccggtgagcg caaagccgat cagcccaaga ataagcccga caaacggaag attcccaaac
     4261 gcgccgagct gctttttcaa aaacttgcca atgccgaaaa agcccgcaac gccaataatg
     4321 ccggccttcc acggggtgga aaggtcgccc cagaagcccc caaacgcccg gccccaatcg
     4381 ccaagcttgt taaacgtccg gcccagccac ccgctcttgg catctgcgcc ggtccagtcc
     4441 agagcccagt taacaccgtc ctcggccatc ttgccgaagc tctcgctcgg gcgcgtaccg
     4501 ccctggctat agggctggtt ttcctgatcg ttcttgcggc tctttttatc agccgggaag
     4561 accttggtaa tggccccttg aaggtctttt ttcgtgtact cgccctcttt accggccagg
     4621 tccagagccc cggtaaacaa cgcgtcgtcc agcaagtcaa attttttgct gatcttcggc
     4681 ccggcatctt gcatgtccca caaaattgcg gtgatcttgc tttgaaggcc gggatcatcc
     4741 ttaaacttcg aggctctaaa cgcaatcacc gcctttgtgc gctcaaactg gagagcttcc
     4801 agagtggtct catgccgctc caaagcagtt tgagcattct ttttggcagt ctccgcatcg
     4861 tcacgggccg ttatgttcac gtcagaatcg tccttaccgg cgattttctt caaacgggca
     4921 tggtctctct gcgcggtctt caatgcgctt tcggcaatct ccacctcggt tttccgcttc
     4981 gcaatgttgg ggtccagcgt gtttctgata aacgccgcgt ccacatcagg cggcacatca
     5041 atcgcctgct gctggtcttt cgcttccgga gtggaagcat cggaaggggt gctatgattt
     5101 tcaacatcat cccagcggtc tcccaatagg gttacaaaac cactcgcaag actgtgcttt
     5161 ttgccaaaaa gatcttttgt ataaggaagg tcttgataca aagaaaggtg tttggattcc
     5221 agccccgtgc cgaacaggcc atcattttcc ttccctaacg ctttttcaat cgcccaatca
     5281 tacgcactaa aagtccagcg agcaggcgtc agagcggcct cgaccggcca cataacagca
     5341 tattctgcaa agcgatttgc aatatcgggc cggaacccca atatatcgat attcccttcc
     5401 tcgtcggtta tccaggtatc attccagtat tctggaatga aatatgaacc ggccgcttca
     5461 agcccttctt ccacagccca agcctgaagg ccaaaataac cgacatttat agccatttca
     5521 tgcgcaaaac ggttagtcat caggtactta gtggtatggt agcctaatct taaagggtcc
     5581 gtagcaaggt tgtatccagg gatacgcgaa agccgtgcgc tccattttgc ccaccctttt
     5641 gcatcttctc ccaccaggcc tgcatacttc catgtcgggt agaagatgct gttttcttca
     5701 tcgatttttt gctgcttaat ctgtccacca aagagcctgc ttttgatata agcttgagta
     5761 gctttgcttc gccacgcagt gacgtacatc tgcccgggtg ttgatattgc gctgcctcta
     5821 cctacaaatt tgcgcgcgtg gtacggtata taactgcctc gggcatacat ggaatgcact
     5881 tctcgctgcg catattcata gggagaatgc gttgcatctt ccagagcgcg tatcgctttc
     5941 tcgtaatgtt catagtgggg tccggcagcc ttacgcaaag ccctcatccc attcagtttg
     6001 gatataaacg ctttgatgag gactcgctca ccgcttacta cgccgtcaga gccttttttg
     6061 atggaccgtt ggttgaggcg tttaaaggcc tcaaggaaca aatgttcatt gccctgtgta
     6121 tatgccgtgt cagaaatccc aagcaatttg tctgccagat tcgcatctgc cggatccatc
     6181 ttctcgacat ccatgatcga ccattccata ttgttgggct gcccgccagg caatgtttca
     6241 tggtaggtac gcttataggg gttatcatat cctatactta gaacattcac tttgcggttg
     6301 atctcaataa ggtcggtatc gggcttggcg gcaccccgca taaaccagaa cagattttgc
     6361 gaggcatcac ggttttctct gcgcatgcgg gcgccacggg tttggttgat caataaatca
     6421 acggtattgg tgaaggcatg gctttgaacg tcggtttccg ggtgtttaag gccttcggca
     6481 agctctttgg cgtattcacc cgtggcgaaa ttatcgtcaa gcaaagaatg cacggtcgct
     6541 tgtaggccct tcgcatcttg gaacatcgca ttggcctgat agctcggaag ataaaaatac
     6601 tgatttttac cgtccattct atcaatgcgc cactgctccc attttttatc agagcctctc
     6661 aactgcgcac cgtttcgaat aaattggatg cgctcttctt tgcctataaa atctcgcccg
     6721 gttgtttgtt cgtagcggtc tataaggact tcagcttcat cggccagttt accggggtcg
     6781 ggcttgacgt cactactttt ttgctgtccg aatttgagca agtctatttt ttcggcaagc
     6841 tggctttcca gtttcatttc cgctgtaatg ccggaaaggg cgtcattgaa ctcttttaag
     6901 gcgactttgt ggtttgttgt aaagttttct acaacgtcaa tcgccatttc ctgagcttgc
     6961 tttttcgtca ggctttccgg tccgccaaca tcgacccggc tggcgatatt tttcaaatcc
     7021 tcgtctaact tgttaaagat gacatcaaga tcttttaacg tgccgttatc gccgataacg
     7081 ctcttggttc ttttagcaat gttttgggct gttcgctgat ttgccccgcc agcgccttcc
     7141 ccggctgatt taccaagaat gagatcgtca actgtattga tatagctgtt gatatatctt
     7201 ttgaaactca actcctgtgg aaggtggcct gccgggttta cggagcccgg aatccatgcg
     7261 ccgattctgg tcacaggaaa ccaatgaatg actttgtgta tccagctacg ctcaggagca
     7321 tcgataaggt ttgccttgtt attcgccttt ttcggtggtg tgtgtgtgag gggaacctca
     7381 tctgcaccag cggttgtcgc ttccggtttt tttgtcaccg cttccggctc caccgcttcc
     7441 accctctctg tcgctttacc atcatccgca gctttgccgg gctttgcccc tgcctctggc
     7501 gctggcgcgc ccgtctcaac ttccgccgcg cgcgtcgtcc ctgctgtctc cgccggagca
     7561 ccaccactgc caccaccaag acgatcaata actttattaa cctgctctct tgttcccgtg
     7621 agtgtcactt caacgtgatc atcctgattt acatctctag aggaattgtt ttttacgcgt
     7681 tctaggtgat caacgacatc cttgggcgaa accctatcct tgggtccgct tgtggggaaa
     7741 tattcactat atttttcttc attctccgcc ttggtatcag gaaactcaac ctctggcggt
     7801 tcagtcgcaa tattgtcggc ttccggcgat gttgccaccg gctcatccgt tttgacaggc
     7861 tccaccaccg gctcatccac cttcggcgta tcggttttca gcgtctcggc ttcggctttg
     7921 gcttcggttt tggcttcggt tttggcttcg gttttggctt cggcttcagg tgccgctgct
     7981 tcatcggcca caccatcggc gttctttttg aattttttgc caaggctgtg cccaagagcg
     8041 ccaccaccgc gtccaaggac ccagccaaat gtaccgccca taaccagagc attcacaccg
     8101 cgccctaaac tatattcata gttattgtca ccaagcgcct gatggccttt attctcaatt
     8161 tcatatgtca gatatctgtc tgaaagcaaa ctttcaacag ctgcgcctgt aatagcggct
     8221 tgtccggctg taacggctgc ctcactcgtc aaggccttgg tcaccgcatg attggcaata
     8281 aattgtgtcg ctttgtgctg ggcaatgttt tgtacaatgg ccgtgtttgc aactttagtg
     8341 gtaatttgtt gcgtaacttt gttcttaagg gctaagccaa aggcgctgcc ggccattttc
     8401 ggagcaagcg ctgccgcacg tgcagccaaa gcagtaccac cagcaaccca acctgcgcca
     8461 cctgagaaaa agcctgttac aaatgatgca ccggcaatgc cccaactcac gtagtccgtg
     8521 gccatggatt tgatgccacg ccacactcct gacattgaag tgtttttatg gtcgataaca
     8581 ttcatcatat agagcatggc aacgttttgg tcagccgttc gattattcaa atcgctatat
     8641 gtgctgatac catcggcaat gcgggtttgc acatcggaca tgtattgcat gccccattcg
     8701 ctataacctt ttttgggtgc tgccgcg
//
LOCUS       DAOSWR010000087         8585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5258_length_8585_cov_6.020985, whole genome shotgun sequence.
ACCESSION   DAOSWR010000087 DAOSWR010000000
VERSION     DAOSWR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_8585_cov_6.020985"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="guaB"
                     /locus_tag="PK513_07180"
     CDS             complement(<1..1457)
                     /gene="guaB"
                     /locus_tag="PK513_07180"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOO82268.1"
                     /translation="MVKIKDPTKIREALTFDDVLLVPGASEVQPNEVKTATQLTQTII
                     LNIPILSAAMDTVSEHQMAIAMAQNGGLGIIHRNMEIEAQVGEVRKVKRFESGMVVDP
                     ITISPDATLEDALLLMQQNNISGIPVTETSGRLVGILTNRDVRFAENPFQPVRELMTA
                     DNLVKAYNTVDKDEAKKLLHKHRIEKLLIVDDAEKCIGLVTVKDIEKSTAFPHATKDT
                     HDRLRAGAAVGTGPVNGVERAAALAAAGLDLVVVDTAHGHSRAVLDTVAQIRADNPDL
                     QIMAGNVATGDAALALIKAGANAIKVGIGPGSICTTRVVAGVGVPQLTAIMDVVKAAS
                     KKNIPVIADGGIKYSGDFAKAIAAGADAAMMGGVFAGTDEAPGEVILYQGRSYKSYRG
                     MGSVGAMVKGSADRYFQAGVTQSNKLVPEGIEGRVPYKGPVGTVIHQMVGGLRASMGY
                     TGCATVAEMKEKAEFVRMTSAGHKESHVHDVTITR"
     gene            complement(1638..2303)
                     /locus_tag="PK513_07185"
     CDS             complement(1638..2303)
                     /locus_tag="PK513_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009571231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOO82269.1"
                     /translation="MSRNKAKERVKSAKGRKSGSTRWLKRQLNDPYVNRAQKDGYRGR
                     AAYKLLEMNEKLDFLKPGQVIVDLGAAPGGWCQVATAKGAKVIAIDLLEMDELADVDF
                     IQMDFMDDDAPDRLKDMISKYNSDGLADLVLSDMAPNTTGHKQTDHLKIMAVVEAASY
                     FAAEVLKPGGTFVAKVRQGGAQNTLLAEMKKDFETIKHMKPPASRKESSEQYLIAQGF
                     RGC"
     gene            complement(2459..2803)
                     /locus_tag="PK513_07190"
     CDS             complement(2459..2803)
                     /locus_tag="PK513_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82270.1"
                     /translation="MSFILQYIDLLWLPLGLLAMHKQHRCWAIGFFIGCMLMMRLQAE
                     LMVSTGYDNGFLGLFEMGAHRRGQITYSIFYVIYIALAVYSPGTKGPIFMAASISIFF
                     AALFTSMIVMLI"
     gene            2960..4288
                     /locus_tag="PK513_07195"
     CDS             2960..4288
                     /locus_tag="PK513_07195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOO82271.1"
                     /translation="MTVIFVFIFPALPNAQAGLPPIIRDTEIEAIFSEWTTPLLKASE
                     IGPDNVRIILVQSDQVNAFVAGGANIFFYTGLIKKTDGPEEVIGVLAHEMGHIAGGHL
                     ITTQDALERASYESILGAVLGLGAAIATGNGAAASAIITGSQNIAGRRFLAHSRINEA
                     SADQAAFSYITRAQLNPSGLSSFFKKLQDQELLPATQQSEYVRTHPITRNRIEAMDAL
                     IAKSPLKDQAPTARWQDQHARMKAKLIGFINPEQTAWAYPKEDTSIPARYARTIAAYR
                     RNDIPTALRGIDELLHDEPHNAYFYELKGQMLVDFSRVEEALPAYRKAVELMPEGPLL
                     RIALAHALLQDEDNRAQVEEAIRHLERALIKEKRSTRVHRLLATAYGRSGRESLAKLH
                     LAEEAVLQQRLPYAKAQAESALKDFRAGSSEWIKTKDVLNHIALLEQQMN"
     gene            4398..5150
                     /locus_tag="PK513_07200"
     CDS             4398..5150
                     /locus_tag="PK513_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOO82272.1"
                     /translation="MRFTFHKTALAFSALTILASGAAGAEEAAFSDAQKDAMGAIIKD
                     YLMENPNVIFEAIEAGRAKQEEEAQKNAEVKIEENIAYLTRAEAPSIGNPDADVTVIE
                     FFDYNCGYCKRALPDIQAAIKDDANLRVVFKDMPILGPTSKTAALWALAAHKQGKYFD
                     YHVALMEHKGPKEEAELEKLARNLGLDVEQMKKDIDSEELQKELDKVTEVGREIGVSG
                     TPAFIVGKTFIPGYVGEDGLKSAINAEREKNK"
     gene            5173..5781
                     /locus_tag="PK513_07205"
     CDS             5173..5781
                     /locus_tag="PK513_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO82273.1"
                     /translation="MIFRIFIGIIALLCAYMAYSTVSFVMLKSAVQKGYEGNVITSPQ
                     ADMSVVEFFDYTCPSCREFQPALMRAVERDGAVRLVLRPIPSDMTDKGKTVARLALSA
                     GKQGKFLEAHKHLIENYRVVDDAYIENFAAALNLDLAQLKEGMKDPEIDDEIDNNLVS
                     LEKLGARGVPTLLLNGDVLYTPNEEGVTSDTILGLFNQARGL"
     gene            5818..6354
                     /locus_tag="PK513_07210"
     CDS             5818..6354
                     /locus_tag="PK513_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOO82274.1"
                     /translation="MKTLLLTFGCVLLGLSANTVQADDLVLGVSAQNAYAYATSPVQQ
                     NGAVFVEFSNITEDDLKITSAKADVSERVELHTHLMDGDMMMMREVDGYDVPAGASVR
                     LEPMGHHIMLMGLKAPLIVGGSFPVTVMDEHGAGLTFTVEVKAPGETMDKPKAHSEHN
                     EEAVESVTDAAQMPETAP"
     gene            6351..6794
                     /gene="aroQ"
                     /locus_tag="PK513_07215"
     CDS             6351..6794
                     /gene="aroQ"
                     /locus_tag="PK513_07215"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019961902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOO82275.1"
                     /translation="MKKILILNGPNLNMLGLREPDIYGDQTLDDIKSLCLEKAQGLDM
                     EIDFRQSNHEGVLVEWIQDAHPRKKGDFDALVINAAAYTHTSIAIHDALKILDIPIIE
                     VHLSNPKNREEFRHNSYIEPLADEVIAGEGAKGYLLALEKLKELL"
     gene            6831..7286
                     /gene="accB"
                     /locus_tag="PK513_07220"
     CDS             6831..7286
                     /gene="accB"
                     /locus_tag="PK513_07220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOO82276.1"
                     /translation="MKIDSKAIKQLADLLEETGLNEIEVADGDKAIRVSKGGGTVVAA
                     ASAPAVSMPSDPAVPQQANTSAPDTTAHTHPGAVTSPMVGTAYLQGEPGAPSFVQKGD
                     NVKAGDTLLIIEAMKVMNPIKAEKGGIVTQILIENGQPVEFGDVLMVVE"
     gene            7296..>8585
                     /gene="accC"
                     /locus_tag="PK513_07225"
     CDS             7296..>8585
                     /gene="accC"
                     /locus_tag="PK513_07225"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOO82277.1"
                     /translation="MFKKILIANRGEIALRIIRACKEMGIKTVAVHSTADASSMAVRL
                     ADESVCIGPAQSKDSYLNIPAILSAAALTGADAVHPGYGFLSENAQFARMVEEHGFAF
                     IGPTPEHIEQMGDKVTAKQTVAALGLPVVPGSDGGVDTPEEGLKIAKNMGFPVIIKAA
                     SGGGGKGMQVVSDPDDFEERFVRARSEAKANFGDDTVYIEKFLGNPRHIEIQVFGDTH
                     GNAIHLGERDCSIQRRHQKLIEEAPSPILSGDLRDEIGCLAADTIRAMGYRGAGTIEF
                     LYEDGKFYFMEMNTRIQVEHPVTEMITGIDLIAEQIRVAAGEPLTVKKERLRLRGHAM
                     EIRINAEDPDNFTPCPGTITQFHAAGGLGVRFDSAIYNGYTIPPYYDSMIGKLIVHGR
                     NREECIRRMKRAIHETIVSGIKTTLPLHDWILRQDAFL"
BASE COUNT         2178 a         1959 c         2244 g         2204 t
ORIGIN      
        1 tcgcgggtga tggttacgtc atggacgtgg gattctttat gtcctgcact ggtcatgcgg
       61 acgaattccg ccttttcttt catttcagcc acggttgcgc agcctgtata gcccatggac
      121 gcgcgtaagc ccccaaccat ctggtgaatg acggtgccga ccggaccttt gtaagggacg
      181 cggccttcga tcccttcggg caccaactta ttcgattgag taacgcccgc ctgaaaatag
      241 cgatcagccg agcctttgac catcgctccg acgctgccca tgccgcggta ggatttataa
      301 gagcggcctt gatagaggat gacttcgccg ggtgcctcat ccgttccggc aaagacgccg
      361 cccatcatcg cggcgtcggc gccggcggca atcgccttag caaaatcgcc ggaatatttg
      421 atgccgccgt cagcgataac gggaatattt ttcttggacg ctgctttcac cacatccatg
      481 atcgcggtga gttgcggcac gccaacaccc gcgacaacgc gggtggtgca gatcgaaccc
      541 ggcccgatgc cgaccttgat ggcattcgca cctgctttga taagggccag cgcggcatcg
      601 ccggtggcca catttccggc catgatttgc agatcgggat tatcggcccg aatttgcgca
      661 acggtatcga gcacagcgcg ggaatggccg tgagcggtat caacaacaac cagatcaagg
      721 ccggcggcgg ccagtgcggc ggcgcgctcc acgccgttga cagggcccgt gccgacagcc
      781 gcgcccgcgc gcaggcgatc atgggtgtct ttagtggcat gcgggaaggc cgttgatttt
      841 tcaatgtctt tgactgtcac caacccgata catttttctg catcatcgac aatgagaagt
      901 ttttcaatac ggtgtttgtg taaaagtttt ttggcttcgt ctttatcgac ggtgttgtag
      961 gctttgacaa gattatcggc ggtcatcaac tcacgcacgg gttggaacgg gttttcggca
     1021 aaacgtacgt cgcggttggt cagaatacca accagacgcc cagatgtttc ggtgacggga
     1081 atgccggaga tgttgttttg ctgcatcaat agcagcgcat cttcaagcgt tgcatccggg
     1141 gaaatcgtta tgggatcaac aaccatgccg gattcaaagc gtttaacttt gcgcacctca
     1201 cccacctgcg cttcgatttc catgttgcgg tggatgatgc cgagaccacc attttgcgcc
     1261 atggcaatcg ccatttggtg ttcgctgacc gtgtccatcg cggcagaaag aatgggaata
     1321 tttaaaataa tggtctgggt aagttgcgtg gccgttttga cttcattggg ctgcacttcc
     1381 gaggcacccg ggacgagcag cacgtcgtcg aacgtcaggg cttcacgaat tttggtggga
     1441 tctttaattt taaccatttg caaattcctc gcacttattg tgggcggaga tccccgcctt
     1501 tcgcgaagac ttcgatttgc aagtgagtta tagcggaaaa atcaaagatg taaagatgtt
     1561 ttgatcttat cagaagattg acaatcagga taatgatgct gcgctatcgt ggaaaattga
     1621 ttttgatccg gtaggttcta acacccccga aagccttgag ctataagata ttgctctgaa
     1681 gattccttgc ggctggcagg cggtttcatg tgtttgatcg tttcgaagtc ctttttcatc
     1741 tctgccagca gcgtgttttg cgcgccgccc tgccggactt tggcgacgaa tgttccgccc
     1801 ggtttgagca cttcggcagc gaaataagaa gccgcttcga ccacggccat aattttgagg
     1861 tgatcggttt gtttatggcc agtggtgttg ggggccatat cggacaggac gagatcagcc
     1921 aaaccatcgg agttgtattt tgaaatcatg tctttgagcc ggtccggagc atcgtcgtcc
     1981 atgaaatcca tctggataaa atcgacatcg gccagttcat ccatttccag caggtcgatc
     2041 gcgatgactt ttgcgccttt ggcagtcgct acctgacacc agcctccagg agctgcacca
     2101 aggtcaacaa taacctgacc gggtttaaga aaatccagtt tttcgttcat ttccaaaagc
     2161 ttatatgccg cgcggccacg ataaccatct ttttgtgcgc gattgacgta agggtcgttt
     2221 aattggcgtt ttaaccaacg tgttgagccg cttttgcgtc ccttagcgct tttgactctt
     2281 tctttggctt tatttctact cacaacactt aaacttcgct tttttgtttt acagatttta
     2341 aagtatgcga acaaacctgc ggtttgctgc tttcttaggt atgcgaacaa acctgcggtt
     2401 tgcttcgcgg gcgcttttga ctctttcttt ggctttatta cggctcatgg ttttcctgtt
     2461 atattagcat cacaatcatt gaggtaaaca aggcagcaaa aaatatagaa atcgaagccg
     2521 ccatgaaaat cggccctttg gtgccgggag aatataccgc caacgcgata taaatcacat
     2581 aaaaaatgct gtaagtgatt tggccgcgcc tgtgagcacc catttcaaac agccccaaaa
     2641 agccgttatc atagccggtc gagaccataa gttcagcctg caaacgcatc atcagcatgc
     2701 agccgataaa aaacccgata gcccagcaac ggtgctgttt atgcatcgca agcaaaccaa
     2761 gcggcaacca gagcagatca atatattgaa gaataaaact catgacttat ttcattattg
     2821 cattcattat ggagccatga tgcgggaaaa atatagaaac atcaaaatgt attcttcgct
     2881 gtcattgcga gcgcggcgaa gcaatccagc cataattgct aaaatccctg gattgcgtcg
     2941 tcggctttgc ctcctcgcaa tgaccgtcat ctttgtcttt atttttcctg ctttacccaa
     3001 tgcacaagcg ggcttgccgc cgattattcg tgatacggag attgaggcga ttttcagcga
     3061 gtggactacg cctttgctga aagcttcgga aatagggccg gataatgtgc ggatcatttt
     3121 ggtacaaagt gatcaggtca acgcgtttgt tgccggcggg gcgaatattt tcttttatac
     3181 cggattgatt aagaaaaccg atgggccgga agaagtcatt ggcgttctcg cccacgagat
     3241 gggccatatt gctggtggac atttgatcac tacgcaagat gcgcttgagc gtgcaagtta
     3301 tgagagtatt ttgggcgcgg ttttggggct tggcgcagcg attgcgaccg gtaacggcgc
     3361 agcagccagt gcaattatta caggcagcca gaatatcgcc gggcggcggt ttttagccca
     3421 tagccggatc aatgaagcaa gtgcggatca agcggctttt agctatatta cccgcgcgca
     3481 gcttaaccct tcggggctgt cgagcttttt caaaaaattg caggatcagg agcttttacc
     3541 ggcaacgcaa caaagcgaat atgttcgcac ccacccgatt acccgcaacc ggattgaggc
     3601 catggacgca ctaattgcca aaagccctct caaagatcaa gcgcccaccg cgcgctggca
     3661 ggaccagcat gcgcggatga aggccaagct cattggtttt ataaatcctg agcaaacagc
     3721 ctgggcctat ccaaaagagg atacatcaat cccggcacgc tatgccagaa ccattgcggc
     3781 ctatcgacgg aatgatattc ccacagcctt gcgcggcatt gacgaacttt tgcacgatga
     3841 accgcacaat gcgtattttt atgagttaaa agggcaaatg ctggtggatt ttagccgcgt
     3901 tgaagaagcc ctgcccgctt accgcaaggc ggttgagctg atgcctgagg gacctttgct
     3961 gcgcattgca ctggcccatg ccttgctgca ggatgaagat aatcgggcgc aggttgaaga
     4021 ggccatccgt caccttgagc gtgctttgat caaagagaaa cgttcaacgc gcgtacaccg
     4081 cctgttggct acagcatatg ggcgctcagg gcgcgaaagt ctggcgaaat tgcatctggc
     4141 tgaggaagca gtgttacaac aacgcttgcc ctatgccaaa gcgcaggccg aaagtgcatt
     4201 aaaagacttt cgtgccggta gctctgaatg gattaaaacc aaagatgtac tcaatcatat
     4261 cgcgctttta gaacaacaaa tgaattgatt taaaaattgt aacatttagg tcattccaaa
     4321 cgaatttcct tttaatatcc atgtggtaag gtacaatgcc ctttgctggt ttaatcaact
     4381 tttgggagag attttttatg agattcacat ttcacaagac cgcgctggct tttagcgcgc
     4441 tgacgatatt agcaagcggt gcggctggtg ctgaagaggc ggcttttagc gatgcgcaaa
     4501 aagacgcgat gggcgcgatc atcaaagatt atttgatgga aaacccgaat gtgattttcg
     4561 aagcgattga agccggacgc gcaaagcaag aagaagaagc gcagaaaaat gccgaggtta
     4621 aaattgagga aaatatcgcg tatttgactc gcgccgaagc tccttctatc ggcaatccag
     4681 acgcagatgt gacggtgatt gaattctttg attataattg cggatactgc aaacgcgctt
     4741 tgccggatat tcaagcggcc attaaagatg acgcgaattt gcgcgttgta tttaaggata
     4801 tgccaattct cgggcccaca tctaaaacag cggcgctttg ggcactggcc gcgcataaac
     4861 agggtaagta ctttgattac catgttgcgt tgatggagca taagggtcca aaagaagaag
     4921 ccgagcttga aaagctggcc agaaatttgg gactggatgt tgagcagatg aaaaaggata
     4981 ttgatagcga agagcttcaa aaagaactcg acaaagttac agaagttggc cgtgaaatcg
     5041 gtgtaagtgg tacgcctgct tttatcgtcg gtaaaacctt tattcccgga tatgtcggtg
     5101 aggatggctt gaaatcagcg attaacgccg agcgcgagaa aaataaataa taatccgggt
     5161 tggtagatct tcatgatttt tagaatcttt atcgggatca ttgctttgct atgcgcctat
     5221 atggcgtata gcacggtttc gtttgtcatg ctcaaatctg cggtacaaaa gggatatgaa
     5281 ggtaatgtta ttacatcgcc gcaagcggat atgagcgtgg ttgagttttt tgattatacc
     5341 tgcccgtctt gtcgggagtt tcaacctgcc cttatgcgag cagttgagcg tgacggcgct
     5401 gtgcgccttg tattacgccc tatcccttct gatatgacag acaaaggaaa aaccgtggca
     5461 cgtttggctt tgtctgcagg taagcaaggc aaatttttag aagcgcataa gcatcttatt
     5521 gagaattacc gcgttgttga cgatgcctat atcgagaatt tcgccgccgc gttaaacttg
     5581 gatcttgcgc agctgaaaga aggcatgaaa gacccggaaa tcgatgatga aattgataat
     5641 aatcttgtaa gtcttgaaaa attgggggcc cgtggtgttc ctacactcct gttgaatggc
     5701 gacgtcttgt acacgcccaa cgaagaaggt gtgaccagcg ataccattct gggcttgttt
     5761 aatcaggcaa gaggattata acttttgtaa ataattttac aaaagtaagg acacatcatg
     5821 aaaactttat tactgacttt tggttgtgtt ttattaggcc tatctgcaaa cactgtgcaa
     5881 gctgatgact tggtcttggg cgtcagtgca caaaatgctt atgcttacgc caccagccct
     5941 gtgcaacaaa atggcgctgt ctttgttgag ttttctaata ttactgagga tgatctcaaa
     6001 atcaccagcg cgaaggctga tgtttctgaa cgtgtggagc tgcatacgca cctcatggat
     6061 ggtgatatga tgatgatgcg cgaagttgat ggttatgacg ttcctgcggg cgcaagtgtt
     6121 aggctggagc cgatggggca ccacatcatg ctgatggggt tgaaagcgcc tttaattgtg
     6181 ggtgggagct tccctgttac cgtgatggat gaacatggtg caggcctgac cttcactgta
     6241 gaggttaaag cgcccggcga gactatggac aagccaaagg ctcatagtga gcataatgaa
     6301 gaggctgttg agagcgtcac ggacgctgct caaatgccgg agactgcacc ttgaaaaaaa
     6361 tcctgatcct gaacggtccc aatctgaata tgttaggcct gcgtgagcct gatatttatg
     6421 gtgatcaaac gctggatgac atcaagagtc tgtgcctcga aaaggcccag gggctggata
     6481 tggagattga ttttcgtcag tctaaccatg aaggtgttct tgttgagtgg attcaagatg
     6541 cacatcctcg taaaaaaggc gattttgacg ctctggtaat caatgcggcc gcttataccc
     6601 atacatcgat tgccatacat gacgcgctca agattttgga tattcctatc attgaggtac
     6661 atttatccaa tcctaaaaac cgcgaagaat ttcgtcataa ctcttatata gagccacttg
     6721 cagacgaagt aatcgccgga gaaggagcaa agggatattt actagccctt gaaaagctca
     6781 aagaactctt gtaagcagga atttattgaa aaacaacaat atgtaagccc atgaaaattg
     6841 attctaaagc gattaaacaa ctggctgatt tgctggaaga aacaggcctg aacgaaattg
     6901 aagtggctga tggtgataaa gccattcgtg tgagcaaggg cggcggtaca gttgttgccg
     6961 ctgcaagcgc tccggctgta tctatgcctt ctgaccctgc tgttcctcag caagcgaata
     7021 ccagtgctcc tgatacgaca gcacacactc atccgggagc tgtaacttcg ccaatggttg
     7081 gaacggccta tctgcagggc gagcccggcg caccctcctt tgttcaaaag ggagacaatg
     7141 tgaaagccgg cgataccttg ctgattatag aggccatgaa agttatgaac cctattaagg
     7201 ctgaaaaagg cggaatcgta acacaaatct tgattgaaaa cggtcagcca gttgagtttg
     7261 gcgatgttct tatggttgtt gagtaagctt ttttcatgtt taaaaaaatc ctcatagcca
     7321 atcgcggtga aatcgccctt cggatcatcc gggcctgtaa agaaatgggc attaaaactg
     7381 tggctgtgca ttccactgcc gatgccagtt cgatggcagt gcggctagct gatgaatcag
     7441 tgtgtatcgg acctgcgcag agtaaagaca gctatttgaa tatcccagca atcctgagcg
     7501 ccgctgcatt gacgggcgcg gatgcggttc atccggggta tgggttcctt tctgaaaacg
     7561 cgcaatttgc acgaatggtt gaagaacacg gctttgcctt tatcgggcca acgcccgaac
     7621 atattgagca aatgggcgac aaggttacgg ccaaacaaac ggtcgcagcg cttggacttc
     7681 cggttgttcc gggctctgat ggcggggtag atacgccgga agaaggcctg aaaattgcca
     7741 agaatatggg ctttccggtg attatcaagg cggcttccgg tggcggtgga aaagggatgc
     7801 aggtcgtatc cgatccggat gattttgaag aacgctttgt tcgagctcgt agcgaagcta
     7861 aagccaattt cggtgatgat acggtttata tcgaaaaatt ccttggtaat ccgcgccata
     7921 ttgaaattca ggtttttgga gatacgcatg gtaacgccat tcatctgggt gagcgagact
     7981 gttcaatcca gcgccgccac caaaagctga ttgaagaggc accatcgccg atcttatcgg
     8041 gagatctgcg tgatgaaatt ggctgtctgg cagctgatac tatcagagcg atgggttatc
     8101 ggggtgccgg aacaattgag tttttgtatg aagacggcaa attttatttc atggaaatga
     8161 atacacgaat tcaggttgaa cacccggtga ctgagatgat cacaggaatt gacttgatag
     8221 ccgagcagat tcgggttgcc gccggggagc ctttgacggt gaaaaaagag cgtttgcgcc
     8281 tgcgtggcca cgcgatggag attcgcatta atgcggaaga tcctgataat tttaccccct
     8341 gccctggtac gatcactcag tttcatgctg ctggaggcct tggtgtacgc tttgatagcg
     8401 ctatctataa tggctatacg atcccgcctt attatgattc gatgattggc aagctgattg
     8461 ttcatgggcg gaatcgcgag gaatgcatcc ggcgcatgaa acgagcaatc catgaaacca
     8521 ttgttagcgg gattaaaacg accctgccgc tgcatgactg gattttacgg caagatgctt
     8581 ttctg
//
LOCUS       DAOSWR010000088         8182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5658_length_8182_cov_5.941922, whole genome shotgun sequence.
ACCESSION   DAOSWR010000088 DAOSWR010000000
VERSION     DAOSWR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5658_length_8182_cov_5.941922"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="flgA"
                     /locus_tag="PK513_07230"
     CDS             <1..905
                     /gene="flgA"
                     /locus_tag="PK513_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOO82278.1"
                     /translation="SFSGIGLANAMASVSLKPSSVIEGDMITLGHVFSGIDRNADKVL
                     GPAPQPGKDMVLNARTLMRIARAMDVNWRPSSAADQVVLRRAATVIPATMIQDALKDS
                     LHAQGLPGKFELIVTSSGLDKIILPQSAAQTVEVESLNFDSEHDHFEAVIAAPSKDDS
                     VFRKNISGQVQRLVDVPVLRAPMKNGSVISASDLDFIALRAYGLNHDMILSADELVGM
                     TPRRMITAGKPMTENDIEPPQIVERGENVTLVFHSGPLSLTAQGKALENGAKGDVIRV
                     VNASSSKMLQARVIGEREVSVSSF"
     gene            945..1697
                     /gene="flgH"
                     /locus_tag="PK513_07235"
     CDS             945..1697
                     /gene="flgH"
                     /locus_tag="PK513_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOO82279.1"
                     /translation="MRNNTTIKIGLILLTCSALGACSAADRIAQIGEAPEMTKIVNPT
                     TQRGYQPVSLPMPSPKNVAPQKNSLWAADRTTFFKDQRASDVGDIITVMIDIDDKAEL
                     ENATSRKRASNEDAALPALLGYEQALDRILPQAIDNTDLIDFGADSSHSGTGSVERDE
                     KVEVQLAAFITQILPNGNMVIHGSQEVLVNFEKRILQVDGVIRPEDISVQNSISYEKI
                     AEARIAYGGEGQITDVQQPRYGQQLYDIVFPF"
     gene            complement(1772..2170)
                     /locus_tag="PK513_07240"
     CDS             complement(1772..2170)
                     /locus_tag="PK513_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82280.1"
                     /translation="MIKVYAHNEAAQAESELNALFGQIATAEDAKALSAKLSDPVKDF
                     ISVRLHSYTFPYASNYLDYLALDFLKIRLNGGDTSLKAYTQENRVYYAELRGALSEVT
                     GSISRRLAGYMRRGDIKNVPRFDQNLHVGL"
     gene            complement(2163..2597)
                     /gene="dksA"
                     /locus_tag="PK513_07245"
     CDS             complement(2163..2597)
                     /gene="dksA"
                     /locus_tag="PK513_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOO82281.1"
                     /translation="MSSTNSVIVPPDYDPENDKGDFMNPVMIEYFRQALIKWRGELVR
                     ESEETIAKTLQGTELQKPDMTDRASEETDHALELRTRDRERKLISKINGTLERIDDGE
                     YGYCEETGEPISIARLKARPIATLSLEAQERHERMEKTQRDD"
     gene            complement(2696..3133)
                     /locus_tag="PK513_07250"
     CDS             complement(2696..3133)
                     /locus_tag="PK513_07250"
                     /inference="COORDINATES: protein motif:HMM:NF022227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HOO82282.1"
                     /translation="MKVQGPGGAKGPSKTGKSGKTSKSDASFGDFIAKDTPAAATTQN
                     TQSIAQVDALLAIQGAEDPTQGAARKRARKRSGVILDELEKIRMAMLGGTLTVGHMID
                     IADVVASHREKIMDPALTDIMDEIDLRAQVELAKMRVALDARN"
     gene            3416..4609
                     /locus_tag="PK513_07255"
     CDS             3416..4609
                     /locus_tag="PK513_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO82283.1"
                     /translation="MLDRAIINDEIITPPAGRSSSPVLAASRAVFAVFVFACLSVFLI
                     TSAHAKTSRIKDIVDIEGVRDNQLVGYGLVVGLNGTGDSLGNSPFTEQSLIAMLERLG
                     VNIRGQNLNTGNVAAVMVTATLPPFVNQGSRVDVSISSLGDAASLQGGTLLVTPLLAA
                     DGEVYAVAQGEVSIAGFSVSGEAATVTQNIPTSGRIAEGAIVEREIDFKLEQLQQVRL
                     ALKNPDFTTARRIAQAINGFTSADLSKAENSASVVLRRPSNYDGTIVDLITDIEQLPV
                     QPDQPARVVISERSGVIVMGADVRISDVAIAQSNLTVKITETPQVSQANPFAEGGETI
                     VVPRTDLAVNTGDDVKLAMMESGVTLQDLVTGLNRLGMKPRDIITIIQAIKAAGALQA
                     EIEVM"
     gene            4609..4989
                     /locus_tag="PK513_07260"
     CDS             4609..4989
                     /locus_tag="PK513_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOO82284.1"
                     /translation="MAGSLTPDTNMALLQASQTNPGKAAKTLKDAAKSHEMQKIEAAA
                     QDFEAVFIAEMMKPMFAGIETQAPFGGGKGEEIFRGMMIQEYGKMIAQTGGIGIADHV
                     KDHMIQVQSQMQNEQNPEGATSDE"
     gene            4982..5464
                     /locus_tag="PK513_07265"
     CDS             4982..5464
                     /locus_tag="PK513_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82285.1"
                     /translation="MNNVSQHQNPASNTLNVLPQEPSMALNMLIRLTNNLSVLADREA
                     QALAQNDMVTFAILQDEKILVTEQYVAASEEFRSKLNTYRGADSSLLDRLERLQRDLG
                     EKTKGNNEVVRQIYGRAQNRTQSALLTAQELGQNHKLRERPAVNENADAKNRKTGETS
                     "
     gene            5461..5949
                     /locus_tag="PK513_07270"
     CDS             5461..5949
                     /locus_tag="PK513_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOO82286.1"
                     /translation="MKHQNQLLSSQKKNFLSADPNKAMQEMMDVIDRMRGVYVRETEA
                     LEDMDTKGFIALQDEKIQATNIYKSGVEDILRRKCEMKAVNPALKRDLERMQADFAAL
                     SSKNMDALKRMQRTMERLGQTVQRAAKESVNKQRAFSYGESGKIHNDERKSVSIGVSE
                     TA"
     gene            5967..7649
                     /locus_tag="PK513_07275"
     CDS             5967..7649
                     /locus_tag="PK513_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOO82287.1"
                     /translation="MSIENFFTTRSFILPAQAQSVAQGKVLPGGQAQGVNFLDFILAQ
                     INPVRDEAQSAGKTAVQNTEDGPLQSDNKLLEKKPELNVAELLAANPEIEEELKDFIE
                     STQLDPEAALTQVLRLNQQAFDNVLKPLTDGIITTAEIAEGSPRILQAFLIEQPQDDS
                     KFIIDINTLKEKIESILEEGGPELIALNLTPEQLDIARSLSEVAPLPPELQDNPFATL
                     VQMIAPVNQPETEAVSFAQEIIQEINEDPALQNLVAILLPPTAQPQPKAQQTLDTTLP
                     PQASSQAYNQMEILAMRLNNLSVGGSTPPPAEGEALFTLEDYEGNLQPSQDGKGQSKG
                     GNNAAFEALMNAESRGNTTPAPSPDLSVLQNWPFPAGSTLFSAFEFGQELTDQYGLNT
                     TSSAPSTLGTLTSAITQAQSAANAHPATQMVAVTLQKAAGAGENKTLTLQLEPPELGR
                     VQVKMTFGADKTVKAVLLSEKPETHMMLQRDAQTLERALQGIELDSDGGLQFELAEHG
                     FDFDQNNQRGGGHDRGGTGAGEDADGVEESIETTMTWQVDPETGHMRYDIVA"
     gene            7669..>8182
                     /locus_tag="PK513_07280"
     CDS             7669..>8182
                     /locus_tag="PK513_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOO82288.1"
                     /translation="MVSDVSVNTALNQQAKTQIASDKLAEDFSQFLTLLTVQLQNQDP
                     LSPMDTTEFTNQLVAFTGVEQQINTNQKLDSLVSLQLGNVMGSALGYVGLDASYISSE
                     FYADGESPVTINYALNDVSVDATIRIESETGDLVYEGPAETSAGSHEFIWDGLDQFGN
                     PAPAGTYNVRV"
BASE COUNT         2088 a         1991 c         2213 g         1890 t
ORIGIN      
        1 ttagctttag cgggattggt ctggccaatg cgatggcttc ggtatctttg aagccaagca
       61 gcgtgattga aggcgacatg attacgctgg gtcatgtgtt ttctgggatt gatcgtaacg
      121 ctgacaaagt tttggggccc gccccgcagc ccggcaagga tatggttttg aatgcgcgca
      181 ctctcatgcg catcgcacgg gcgatggatg tgaattggcg cccctcctcg gcggccgatc
      241 aggttgtgct tcgccgcgcg gcgacggtga tcccggccac aatgattcaa gatgcgttga
      301 aagacagcct gcacgcgcaa gggctacccg gaaaatttga actgattgtt acaagttcag
      361 ggcttgataa aattatcttg ccgcaaagcg cggctcaaac tgttgaagtt gagagcctga
      421 atttcgattc cgagcatgat cactttgaag ccgtgattgc tgcgccttca aaggacgatt
      481 ctgtgtttcg caaaaatatc agtgggcagg tgcaacggct cgtcgatgtt ccggtcctgc
      541 gcgcgccaat gaaaaacggc tcagtgatta gcgcaagtga tttggacttc atagccttac
      601 gggcatacgg actgaaccat gacatgattt tgagcgccga tgagttggtc ggcatgacgc
      661 cgcggcgcat gatcacagca ggtaagccga tgaccgaaaa tgatattgag ccgccgcaaa
      721 tcgttgagcg cggagaaaat gtcactctgg tttttcatag cggacctttg tctctcaccg
      781 cgcaaggcaa ggctctggaa aacggtgcca aaggtgatgt gatccgcgtg gtaaatgcga
      841 gttccagcaa aatgttgcaa gcgcgtgtta ttggtgaacg cgaggtcagc gtttcaagtt
      901 tttaatttta aggactgttg aatacagcag aaagggtgag ctctatgcgc aacaatacaa
      961 caataaaaat cggtctgatc ctgcttacat gcagcgctct gggcgcatgc agcgcggcgg
     1021 accggattgc acagatcggc gaagcgcccg agatgacaaa aattgttaac ccgacgacgc
     1081 aacgcggcta tcaaccggtg agcctgccga tgccgagccc gaaaaatgta gcaccgcaaa
     1141 aaaactcttt gtgggcggcg gaccggacga cattctttaa agaccagcgg gccagcgatg
     1201 tcggcgatat tatcacggtt atgattgaca ttgatgacaa agccgagttg gaaaatgcaa
     1261 cctcgcgtaa acgcgccagc aatgaagatg cggcgctgcc tgctttgcta ggctatgagc
     1321 aagctttgga tcgcatattg ccgcaagcga ttgataatac tgatctgatc gactttggcg
     1381 ctgattccag ccatagcggc acgggaagtg ttgagcgcga tgagaaagtt gaggtacaac
     1441 tcgctgcgtt cattacgcaa attctgccta acggcaatat ggttattcat ggttctcagg
     1501 aagtgctggt gaattttgaa aagcgcattt tgcaggttga tggcgtgatc cgtccggaag
     1561 atatttccgt ccagaacagc atttcttatg agaaaattgc cgaggcgcgc attgcctatg
     1621 gcggtgaagg gcaaattacc gatgtgcagc agccgcgcta cgggcagcag ctctacgaca
     1681 tcgttttccc gttctaagaa tagacgaata ccttacccac aactcccggc cggctttggc
     1741 gtgttaaacg tcatggccgg tttttttatg tttacagtcc aacatgcagg ttctggtcaa
     1801 accgggggac gttctttatg tcgccccttc tcatataccc tgcaagtctt cgggatatcg
     1861 aacccgtcac ctcagacaaa gctccacgta attctgcata gtataccctg ttttcctgtg
     1921 tatacgcttt taatgatgta tctcccccat tcagcctgat tttcagaaaa tccaaagcca
     1981 ggtaatctaa atagttgctt gcatatggaa acgtataact atgaagtcgt acggagataa
     2041 aatcctttac cggatcagaa agttttgctg acaatgcctt cgcatcttct gctgttgcta
     2101 tttgtccaaa aagtgcattc agttcgcttt cagcctgcgc tgcctcgtta tgcgcatata
     2161 ccttaatcat cgcgctgggt tttttccatg cgctcgtggc gttcctgcgc ttccagagaa
     2221 agcgtggcga tggggcgcgc cttgaggcga gcaattgaga tcggctcacc ggtttcttca
     2281 caatagccgt attcgccgtc atcgattctt tcgagtgtgc cgttgatttt ggaaatcaat
     2341 tttcgttcac ggtcacgggt gcgcagctcc agcgcgtggt cggtttcttc agatgcacga
     2401 tccgtcatgt ctggtttttg aagttcggtg ccttgcaaag ttttggcaat ggtttcttca
     2461 gattcacgaa caagctcacc acgccatttg ataagggcct gtcgaaaata ttcgatcatc
     2521 accgggttca taaaatcgcc tttgtcattt tccgggtcgt agtccggggg gacaatcaca
     2581 ctgttggttg aactcatggt ttttcaaatc cctattgctt aaacacatta atcaaggcga
     2641 agactatatt gaagggttga gccagtgaca agcaaaacta tcgctgcagg cgcaattagt
     2701 tgcgagcatc aagcgccacg cgcattttag ccagctcaac ctgagcgcgc aggtcaattt
     2761 catccataat atcggtcagg gccgggtcca tgattttttc gcggtgggaa gcgacgacat
     2821 cggcgatgtc gatcatatga ccgacggtca gcgtgccgcc gagcatggcc atgcggattt
     2881 tctccagctc atcgagaatg acaccggagc gcttacgggc gcgctttcgc gcagcgcctt
     2941 gcgtggggtc ttcggcaccc tgaatggcga gcagcgcatc gacctgggcg atggactgcg
     3001 tattttgcgt ggtggccgca gcgggcgtgt ctttggcgat aaaatcgccg aagctggcat
     3061 cgcttttgga ggttttgccg ctttttcctg ttttagaggg gccttttgcg ccgccagggc
     3121 cttgtacttt catacgcgtt tctcaccgtt attttttttc actatagcgc gttttgttta
     3181 gaaccggaag atcggtgtgt ggataagtcg gcggaaagat ttgccgcccc gatcattttt
     3241 gcctggcttt aaatccataa acaataaaca cccccaccct aaccctcccc ctcaaggggg
     3301 agggagctta tggggcagga aaaatagaga aataacaaaa ctggcacgga cttcgcatag
     3361 aaggatatgc aggatatttt ttcctgccct gaaagcaagc aagagtaaga aacatatgct
     3421 ggacagagcg attataaatg atgagatcat aacaccgcct gcgggacggt cctcctcccc
     3481 cgtattggca gcctcccggg cggtgtttgc cgttttcgtt tttgcatgcc tcagcgtttt
     3541 tcttattacc agcgcgcatg ccaagacctc acgcatcaag gatattgtcg atattgaagg
     3601 cgtgcgcgat aaccaactgg ttggttatgg ccttgtcgtg ggactcaacg gcacaggcga
     3661 ttccttaggg aactccccat ttaccgagca gagcctgatt gcgatgctgg agcgtctggg
     3721 cgtcaatatc cgcggacaga atttgaatac cgggaatgtg gccgccgtta tggtgacggc
     3781 caccctgccc ccgtttgtca atcaaggctc tcgcgttgat gtaagcattt cttcgctggg
     3841 cgatgcggca agcctgcagg gcggaacttt gttggtgacg cctttgctgg cggcggatgg
     3901 cgaagtttat gccgtggccc agggcgaggt gagtattgcg ggctttagcg tttccggtga
     3961 ggcagcgacg gtgacgcaga atattccgac ctccgggcgc attgccgaag gtgcgattgt
     4021 cgagcgcgaa attgatttta agctggaaca gttacagcaa gtacgtctgg ctctcaaaaa
     4081 ccccgacttc acaacggcac ggcgaattgc ccaagctatt aacggcttta cctctgctga
     4141 tttatcgaag gcagaaaatt ctgccagcgt ggttttgcgt cgtccctcta actatgacgg
     4201 cacgattgtc gatcttatca ccgatattga gcaattgcct gtgcagcccg accaacctgc
     4261 tcgcgtagtg atttcagagc gctccggcgt gatcgtgatg ggcgcagatg tgcggattag
     4321 cgatgtggcg attgcgcaga gcaatttgac ggtcaaaatt accgaaacgc cgcaggtttc
     4381 tcaagccaac ccgtttgccg aaggtggcga gaccatcgtt gtgccgcgca cggatctggc
     4441 ggtgaatacg ggtgatgacg tgaagctggc gatgatggaa agcggcgtca ctttgcagga
     4501 tctggttaca gggctgaacc gtcttggtat gaaaccgcgt gacattatta ccatcataca
     4561 ggccattaaa gccgccggcg cgcttcaggc agaaattgag gtgatgtgat ggccggctct
     4621 cttactcccg atacgaatat ggcgctgttg caggcttcgc agaccaaccc gggcaaggcc
     4681 gcaaaaacgc ttaaagatgc ggccaagtcg catgaaatgc aaaaaattga agcggcggcg
     4741 caggattttg aagcggtgtt tattgccgaa atgatgaaac cgatgtttgc gggcattgaa
     4801 acacaagctc cgtttggcgg gggcaaaggc gaagagattt tccgcggcat gatgattcaa
     4861 gaatacggca agatgattgc acagaccggc gggatcggca ttgccgacca tgtgaaggac
     4921 cacatgatcc aagtacaaag ccaaatgcaa aatgaacaaa accctgaagg agcgacaagc
     4981 gatgaataac gtttctcaac atcaaaatcc tgcgtcaaat actctcaatg tcctgccgca
     5041 ggaaccttcc atggcgttga atatgctgat ccggctgacg aataaccttt ctgttttagc
     5101 cgatcgggaa gcgcaagctt tggcacaaaa tgacatggtt acttttgcca tccttcaaga
     5161 cgagaaaata cttgtaactg aacagtatgt ggcggcttct gaagaattta gaagcaagct
     5221 gaacacttat cgcggggcag atagctccct gctcgatcgg ctggagcggc tgcagcgcga
     5281 tctgggtgaa aaaaccaaag ggaataacga agtcgtacgg caaatttacg gtcgggcgca
     5341 aaatcgtacg cagagcgccc ttcttaccgc gcaggaactg ggacaaaatc acaagctgcg
     5401 cgaacgcccg gctgttaacg aaaatgcgga tgcgaaaaac cgtaaaacgg gagagacatc
     5461 atgaagcatc aaaatcaact tttatcttcc cagaagaaaa acttcctttc cgcagatccg
     5521 aataaggcca tgcaggaaat gatggatgtg atcgaccgga tgcgcggcgt ctacgttcgc
     5581 gaaaccgaag cgctggaaga tatggatacg aaaggcttta tcgccctgca ggatgagaag
     5641 atacaggcaa ccaatatata taagtcaggt gtcgaagata ttttacgccg caaatgtgag
     5701 atgaaggccg ttaatccggc gctgaaaaga gatctggagc ggatgcaggc cgattttgcc
     5761 gcgttgtctt ccaagaatat ggacgctctg aaacgcatgc aacgcacgat ggaacgattg
     5821 ggacaaaccg tgcagcgcgc agctaaagag tcggtgaata aacagcgtgc ttttagctat
     5881 ggcgagagcg gaaaaattca taacgacgaa cgcaagagcg tttcaatcgg cgtcagcgag
     5941 acggcctaga aggacctatg agtgatatga gtatcgagaa cttttttaca acccgatctt
     6001 ttattttgcc cgcgcaggca caaagcgtgg cgcagggcaa agtcctgccc ggcgggcagg
     6061 cgcagggcgt gaattttctt gatttcattc tggctcaaat caacccagtt cgtgacgagg
     6121 cacaaagcgc ggggaaaacc gccgtacaaa acactgaaga cggcccctta caatcggaca
     6181 acaagttgct ggagaaaaaa ccagaattga atgtcgctga acttctggcg gccaaccccg
     6241 agattgaaga agaactgaaa gactttattg agagcacgca acttgatccc gaagcagccc
     6301 ttacgcaggt tttacgtctc aatcaacagg cgtttgataa tgttttaaaa ccgcttacgg
     6361 atggcattat cacgacagca gaaatcgccg agggtagccc gcgcattttg caggctttcc
     6421 tgattgaaca accacaagat gatagtaaat ttattattga tatcaatacg ctcaaggaaa
     6481 aaattgaatc aatcctagaa gaaggcggac cagagctgat cgcattgaat ttaacaccgg
     6541 aacagcttga tattgcacga agtttatcgg aagtggcgcc attaccccct gaactccaag
     6601 acaacccgtt tgctacgttg gtgcagatga ttgcgccggt caatcaacca gaaacggagg
     6661 ctgtatcctt cgcgcaagag atcattcagg agatcaacga ggatccggcg ctgcagaatc
     6721 tggttgcgat tttattgccg ccaacagccc agccacaacc aaaggcgcag caaacactcg
     6781 atacaacctt accgccgcaa gcctcttcgc aagcatacaa ccaaatggaa atactggcga
     6841 tgcggttgaa taatttaagt gttggcggta gcacaccgcc gcctgccgaa ggcgaagcgt
     6901 tgtttactct ggaagattat gaaggcaatt tacaaccctc acaagatggc aaaggtcaga
     6961 gtaagggggg aaataacgcc gcctttgaag cgctgatgaa tgcggaatca aggggcaaca
     7021 cgacccctgc cccatcgcca gatttaagcg ttttgcaaaa ctggccgttt ccggctggca
     7081 gcacgttatt ttccgcattt gaattcgggc aggaacttac agatcaatat gggttgaaca
     7141 caacctcaag cgcgccttct acgctcggca ccctgaccag cgcgattaca caggctcaaa
     7201 gcgcggcgaa cgcgcatccg gctacgcaga tggttgccgt aaccctgcaa aaagcggctg
     7261 gcgctggcga gaacaaaacc ctgacgctgc agcttgagcc gccggaactg gggcgggtgc
     7321 aagtcaagat gacatttggc gcggacaaga cggttaaggc tgtcttgctg tctgaaaaac
     7381 ccgaaacgca catgatgctg caacgtgatg cgcagacttt agagcgggca ttgcagggga
     7441 ttgaactgga cagcgatggc ggactgcaat ttgaactggc cgaacatgga tttgattttg
     7501 atcagaacaa ccagcgcggc ggcgggcacg atagaggcgg cacaggtgcg ggcgaagacg
     7561 ccgacggcgt tgaagagagt attgaaacca caatgacatg gcaggttgat cccgaaacgg
     7621 gacacatgcg ctatgacatc gtggcgtaaa atttaaggag aaaaccggat ggtttcagat
     7681 gtatcagtta atacggcgct taatcaacaa gccaagacgc agattgcatc agacaagctg
     7741 gccgaagatt tcagccagtt cctgacactg ctgaccgtgc agctgcaaaa ccaggacccg
     7801 ctctcgccca tggacacaac tgagttcacc aatcagcttg tcgcctttac cggggtggaa
     7861 caacagatca acaccaacca gaaactcgat tcactggttt ctctgcaact cggcaacgtc
     7921 atggggtcgg ccctggggta tgtcggcctt gatgcgagct atatttcttc tgaattttat
     7981 gcggacggag aaagtccggt cacaattaat tacgccctga atgatgtatc ggtcgatgcg
     8041 acaatccgta tagagagcga aacaggtgat cttgtttatg agggaccggc ggaaacctca
     8101 gccggcagcc atgaatttat ctgggacggg ctggatcaat tcggtaaccc ggcccctgcc
     8161 ggaacataca atgtgcgggt tg
//
LOCUS       DAOSWR010000089         8095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5733_length_8095_cov_4.832463, whole genome shotgun sequence.
ACCESSION   DAOSWR010000089 DAOSWR010000000
VERSION     DAOSWR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5733_length_8095_cov_4.832463"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..849
                     /locus_tag="PK513_07285"
     CDS             58..849
                     /locus_tag="PK513_07285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO82289.1"
                     /translation="MSTLPQEKERQEEFPWQRGKFHTEYNAFLAHYKVMSCLEHAKGK
                     SLLDLACGDGLMTEMFSHHFDTIVGVDASSKHVAEARKRLPNAEFHEALIEDFETEQK
                     FDSVFLLDILEHVIDPVAVLKKAASFLNDDGVLIMHVPNAQAINRRLAVLMGTLTSCE
                     ELSPFDINIAGHRRAYDLESLTKEVENAGLTVTKTGGIFYKILSTPQMDWFLKEGLWD
                     EGHGWGRTGQEKAKDWKAEFCRASYELGKLHPNDCNIIFACATRK"
     gene            868..1683
                     /locus_tag="PK513_07290"
     CDS             868..1683
                     /locus_tag="PK513_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin hydroxylase family protein"
                     /protein_id="HOO82290.1"
                     /translation="MNQILTFKKEVENNLEILSKDTSLQAFAMEFLKESGLRNYSYNF
                     TALGRPIIQYPQDMVAMQELIWSVRPDLIIETGIAHGGSLIANAAALAQLDYCDAVQA
                     GKTLDPKASKRKVLGIDIDIRAHNRAEIEKHPMVHHIDMIEGSSIAPDMVAKVYDYAK
                     DFSTILISLDSNHTHDHVLAELKAYAPLTSIGSYCIVYDSSIEDMPEDMYNDRPWGKG
                     NNPKTALHKYLKYLGNEDIKAADGKRLKLEIDKRIESKILITVAADGYLKRIS"
     gene            1917..2639
                     /locus_tag="PK513_07295"
     CDS             1917..2639
                     /locus_tag="PK513_07295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOO82291.1"
                     /translation="MFTKSSLSSVGNTFAAPLSNTGAAISKPLSLYQKFHDNFSIILA
                     DTPELRKDAMRIRYLAYCVENPFENPEDNLEGLEQDVYDEKAHMALVRHNKSGINIGT
                     TRLIVKDEKHALPIEGVVHDEVVKSVKHMSYNVGEISRFCIAQTRKKKIDDDSAILNL
                     GFLGIVAGSFMLCRTHNITHWVGAMEPFLFKKLEMIGLKMDYVGETFLYHGERRPFFS
                     DISNILEKMSYTHNDVWHLMAR"
     gene            2685..4712
                     /locus_tag="PK513_07300"
     CDS             2685..4712
                     /locus_tag="PK513_07300"
                     /inference="COORDINATES: protein motif:HMM:NF006077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOO82292.1"
                     /translation="MRPFLAKPLAEAGSPFDYETAFSRNLGFLTESEQEVLRSKRVAI
                     PGMGGVGGFHLLTLVRLGIGSFNISDMDVFEVANFNRQIGADMSTIGFEKSEVMAEKA
                     RLINPELDIKTFPEGISKDNLDAFLEGVDLAIDGFDVFVLDMRSLFFEACYDKGIPVI
                     TAGPVGMGTAYLNFMPGKMSFADYFGISREMSEEEKIIHFVAGLLPVPLHWASLVEPW
                     RLDINAKKAPSTPMGCSLAAGVAGAEALKILLGRGKLKAAPHYHHFDAYTGRWKNGFI
                     PFGAKNPLQRLKLHMEKKHLADLKQQEKLRDTQKPIETDDEIVAILDMARWAPSGDNS
                     QPWRFERTGDSAVRIHLRLGGDNNVYELGDGIPVYQSAGTLLESARLAAADFGRKLVW
                     ALEDECETGFKIAVDLPKDASVQPEKLAKFIKERSVNRYPFKLKSLDDTAIQALEQSV
                     GDDFSIQWHLDLKDRWNIVKVNAIGTDIRLSIPEAYPVHKKILDWDHKLSENAIPDAA
                     TGLSPLTRMLMRKIMKKWERVRFMNTYMGGTLMPRLEMDVLPGLLCAAHFTLFAKSEK
                     KPTKEDFIASGMAIQRFWLTATELGLALHPGYAPIVFSFYGRNKIDFTKDGRARKMAE
                     LLDKKCTEIWNGKPEHVRFQGRIGYAQARKAFGRSIRKPLDDLWYRGEKTK"
     gene            complement(4714..4803)
                     /locus_tag="PK513_07305"
     tRNA            complement(4714..4803)
                     /locus_tag="PK513_07305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4767..4769),aa:Ser,seq:tga)
     gene            4906..5349
                     /locus_tag="PK513_07310"
     CDS             4906..5349
                     /locus_tag="PK513_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOO82293.1"
                     /translation="MLIQFAIGAFLIGLTVLIHALALDRLMYTLEEHSNRIFMWFRML
                     WKAAMLTIAVLGVFSAHIVQIWIWAVFYLMVDALPDMESALYFSTSTFTTVGFGDVVL
                     GEDWRLISSFQSANGFILFGWSTAFIFEVMSKLYHDESMRKNRKS"
     gene            5353..6312
                     /locus_tag="PK513_07315"
     CDS             5353..6312
                     /locus_tag="PK513_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOO82294.1"
                     /translation="MRIKRFLLIALLLPVSAQAAQPFDQWLKDLRHEAASKGISNKTI
                     DVALTGIEPIPRVIELDRKQPEGRMTFNEYYHKVINQQRIDQGRTMYRKHRAELERAA
                     RKYGVPANYIVALWGIETNYGSNTGGFKVVPALATLAHDGRRAEFFRNELMYALKILD
                     QGHISPADMKGSWAGAMGQNQFMPSSFHAFAVDGNNDGRRDIWASLPDVFASTANYLS
                     KSGWKADQRWGREVKIPQDFPESLTGLKVKKSLDEWKTMGITLPSGENLPTAPGIRAS
                     LVAPDGLNGRVFLVYDNYHVIMRWNKSTYFATSVGLLANQVAQ"
     gene            6382..7320
                     /locus_tag="PK513_07320"
     CDS             6382..7320
                     /locus_tag="PK513_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOO82295.1"
                     /translation="MITILYAGLLSGCAEIELASHVAKKAPIPATSKSKGTFKVGSSY
                     KVAGQWYKPRETYSFEQTGVASWYGPNFHGKKTANGETFDMYEITAAHKTLQMPSLIR
                     VTNLENGRSIIARVNDRGPFSKNRILDVSKRGAELLGFKNQGTARVKIQVLAKESRHA
                     AAIAKQGRSTRGMEVAMNERNYQPASHRLTTVPSSRQPTRQPDVETHTVAALQPVEAI
                     PLNNAHASAAIRHATQQQAQSIYVQAASFGDPVKAKSVADTLKDFGDAHVQRAVVNGR
                     NYFRVRIGPLQNEPQANQVVARLASAGRDDAIVVTD"
     gene            7419..>8095
                     /locus_tag="PK513_07325"
     CDS             7419..>8095
                     /locus_tag="PK513_07325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO82296.1"
                     /translation="MLKLCTLALSLVMFCGLPVLAAHAASIETKAKQAIVVDYETGTV
                     LLSKNMDEQMPTSSMSKVMTMYAVFDSIKNGQISLNDTLPVSEKAWRMGGSKMFVEVD
                     KRVKVEDLIRGVIVQSGNDATVVLAEGLAGSENAFANMINDKATGLGMKNSHFVNASG
                     WPDPEHYSTARDLATLGRAIIHDFPDYYKYYMEKEFTYNNIKQTNRNPLLYRNIGADG
                     IKTGHTEA"
BASE COUNT         2297 a         1883 c         1888 g         2027 t
ORIGIN      
        1 cattgacgaa ctcctgaaga gccacaatat caaattagaa aagaacaagg gtataaaatg
       61 agcactctgc cccaggaaaa agaaagacaa gaagaattcc cttggcagcg gggtaaattc
      121 cacacggaat ataatgcatt tctggcccac tataaggtta tgtcgtgcct tgagcacgca
      181 aaaggaaagt ccttgctgga tttggcctgt ggcgatggcc tgatgacgga aatgttttca
      241 catcactttg acaccattgt tggcgttgac gcctcatcaa agcacgttgc agaagcaaga
      301 aaaagattgc ccaatgctga atttcacgaa gccctgattg aggattttga aacagaacag
      361 aaatttgatt ctgttttttt attggatatt ctggaacatg tgatcgatcc agtcgcagtc
      421 ttaaaaaagg cagccagctt tttgaatgat gatggtgtgc taattatgca tgtaccaaat
      481 gcgcaggcaa ttaaccgtcg tttggcagtt ttaatgggaa cgcttacaag ctgcgaggag
      541 ctatcgccat ttgatattaa tattgctggt caccgcagag cgtatgactt ggaaagcctc
      601 actaaagaag tagagaatgc tgggttaact gttacaaaaa caggcggtat cttctacaaa
      661 attttatcaa caccacaaat ggattggttc ctcaaagagg ggttgtggga tgaggggcat
      721 ggctgggggc gtacgggtca ggaaaaagcc aaagactgga aggctgagtt ttgtagggca
      781 tcttacgaac ttggtaaact gcatccaaac gactgcaaca ttatttttgc atgcgccacg
      841 cgtaaataaa tagaaattag gtacgatatg aaccagattt taacgttcaa gaaagaggtt
      901 gaaaataacc ttgaaattct ttcgaaggac acatctctcc aagcttttgc catggaattt
      961 ttgaaggaaa gcggcctaag aaattattcc tataatttta ctgcgttggg aagacctatc
     1021 attcaatatc cgcaagatat ggtcgccatg caggaattga tctggtccgt acgtcccgat
     1081 cttattattg agacgggaat tgcgcatgga ggttcgctaa tagccaatgc cgcagccctg
     1141 gcacaactgg attattgcga tgcagtgcaa gcagggaaga cacttgaccc taaggccagc
     1201 aaacgtaagg ttcttggcat cgacatagat atacgcgccc ataacagggc agagattgaa
     1261 aaacacccca tggtgcacca tatcgacatg attgagggtt catcaatcgc cccggatatg
     1321 gttgccaaag tgtacgacta cgccaaagac ttttctacaa tattaatatc actggattcg
     1381 aatcatacac acgaccatgt attggcagaa ttaaaggcat atgctcctct tacaagcatt
     1441 ggtagctact gcatcgtcta tgatagtagt atcgaagata tgccggaaga tatgtacaac
     1501 gatcgcccct ggggcaaagg caacaatccc aaaacagccc tgcacaaata tttgaaatat
     1561 ctgggaaacg aagacattaa agccgctgac ggtaaaaggc ttaaactgga aattgacaag
     1621 cgtatcgaaa gtaaaatctt gataactgtc gcagccgacg gttacctgaa gcgtatctcc
     1681 tgatttttta gatcgggcgc tgtatctgaa cacgttacga gcgatatgct cgcactttcc
     1741 aaactgccat cgctatcgct gctatcatct tataatatat acagaattcg tcctatatat
     1801 gggcacagac gctctttcaa acgcaatatt taaaatatta tggaatatta aggttttttt
     1861 atcacttgtt ccgcatacta tactatgtgc tatgacaaaa ataatggggt atatctatgt
     1921 ttacaaaatc gtccttatcc tctgtgggga atacttttgc tgcgccttta tcaaatacgg
     1981 gcgctgctat ctcaaagccg cttagtttat atcagaaatt tcacgataat ttttcaatta
     2041 ttttagcgga tacaccggaa ttgagaaaag atgctatgag aattcgctat cttgcctact
     2101 gcgttgaaaa tccttttgaa aatccggaag ataatctgga aggattggaa caggacgtct
     2161 atgatgaaaa ggcgcatatg gcgcttgtaa gacataataa aagcggtatc aatatcggta
     2221 ctacacgtct gattgtaaag gatgagaaac acgctcttcc tatagaaggt gtcgtgcacg
     2281 acgaggttgt taaatccgtt aagcacatgt cttataatgt tggtgaaatt tcaagatttt
     2341 gcattgcgca aacaagaaag aaaaagatag atgacgactc tgccatactt aatctgggct
     2401 ttttggggat tgtggccggc agcttcatgc tatgcagaac gcacaatata acccattggg
     2461 taggcgctat ggagccgttt ttattcaaaa agctcgagat gattggactg aaaatggact
     2521 atgtggggga aactttcctt taccatgggg aaagacgtcc ttttttctca gatatatcca
     2581 atattcttga aaaaatgagc tatactcaca atgatgtttg gcatttaatg gcccgataat
     2641 gatataacag gcctgtgacc gaaacgtgag gaggctttat tattatcaga ccttttttag
     2701 caaagcctct tgcagaggca ggtagccctt tcgactatga gaccgccttt agccgcaatc
     2761 tcggttttct cacggagagt gagcaggagg ttttgcgttc caagcgtgtc gcgattcccg
     2821 gcatgggggg cgttggcggt tttcatcttt taacactggt cagacttggc ataggatcat
     2881 ttaacatctc ggatatggat gtttttgagg tagcgaattt caaccgtcag ataggagccg
     2941 atatgagcac gatcggcttt gaaaaaagcg aagtcatggc ggaaaaagcg cgccttatca
     3001 accccgaact cgatataaaa acctttcccg aaggcataag caaggataac ctggacgcat
     3061 ttttggaagg ggtggatctg gccattgacg gtttcgatgt cttcgttttg gatatgcgct
     3121 ctcttttctt tgaggcctgc tatgataaag gaattccagt tattaccgca ggtcccgttg
     3181 gcatggggac agcttatttg aattttatgc ccggtaaaat gagtttcgcc gattattttg
     3241 gcatctcaag ggaaatgtcc gaagaggaaa aaattataca cttcgtcgca gggcttcttc
     3301 cggtgccttt acactgggca tcgcttgtcg aaccgtggcg gctcgatatc aatgcgaaaa
     3361 aggccccctc aacgcccatg ggctgttcgt tggcggcagg tgttgccggc gcggaagcac
     3421 ttaaaattct tttaggaaga ggaaagctca aagcagcgcc tcattaccac catttcgatg
     3481 cgtatacagg aagatggaaa aacggtttca tcccctttgg cgcgaaaaac cctttacaaa
     3541 ggctgaaatt acatatggaa aaaaaacatc tcgcagatct caaacagcaa gaaaagctta
     3601 gagatacaca aaaacctatc gaaacagatg acgaaatcgt agcgattttg gatatggcgc
     3661 gctgggctcc gagcggggac aacagccagc catggcgttt tgagagaacc ggggacagcg
     3721 ccgtacgcat tcacctcaga cttggtggcg acaataacgt ttacgaactc ggcgacggca
     3781 ttcccgtata ccaaagcgcc ggcacgcttt tggaatctgc gcggttggcg gcagcggatt
     3841 ttggccgcaa gctggtctgg gctcttgagg acgagtgtga aaccgggttt aaaattgccg
     3901 tcgacttgcc taaagacgcc tccgttcaac cggaaaagct tgcaaaattt atcaaagaac
     3961 gtagcgttaa tcgctatccg tttaaactca aaagccttga cgatacggct attcaggcct
     4021 tggagcaaag tgtcggcgac gatttttcaa ttcagtggca tttagatcta aaagatcgct
     4081 ggaatatcgt taaagtcaac gctatcggca cggacattcg cctgagcatc cctgaggcct
     4141 accctgttca caaaaagata ctcgactggg atcataagct gagtgaaaac gctattccag
     4201 atgccgccac cggtctctcg ccgttaacac gcatgcttat gcgtaaaatt atgaaaaaat
     4261 gggaaagagt ccgttttatg aacacgtata tggggggaac cctcatgcct cgccttgaga
     4321 tggacgtttt accggggcta ttgtgcgccg ctcattttac actttttgcg aaaagcgaaa
     4381 aaaagccgac aaaagaggat tttattgcat cgggcatggc catacagcgc ttctggctaa
     4441 cggcgaccga attaggattg gcgcttcatc ccggttacgc tccaattgtg tttagcttct
     4501 atgggcgcaa caagatagat ttcacgaaag acggacgtgc ccgcaagatg gctgaactcc
     4561 tggataaaaa atgtaccgag atatggaacg gaaagcctga gcatgtcaga tttcaaggac
     4621 gcataggcta cgctcaagct cgaaaggcgt ttgggcgctc catcagaaaa cctcttgatg
     4681 atctatggta ccgcggggag aagaccaaat aaatggcgga gggagtggga ttcgaaccca
     4741 cggtagagtt acccctacgg cagttttcaa gactgctgcc ttcaaccact cggccatccc
     4801 tccttaaata tgtgcaagcc tttagatgac gacttgtcag tgcatgactt tttagcgata
     4861 tttataagtc tgtgtccaga aaaaatatca acgaaagccg aaattttgct catacaattt
     4921 gccattggcg cctttctaat cggcttaacg gtgctcatcc acgctctggc actggatagg
     4981 ctgatgtaca cactggagga acacagcaac cggattttca tgtggttccg tatgctttgg
     5041 aaagcagcca tgctcacaat cgcggttttg ggcgtatttt cggcccatat tgtacaaatt
     5101 tggatttggg ccgtttttta tctgatggtt gatgcgctgc ctgacatgga atccgcactg
     5161 tacttttcga cctcaacctt cacaaccgta ggcttcggcg atgtcgtatt gggggaggac
     5221 tggcgattaa tcagttcatt tcaatccgca aatggtttta tattgtttgg ttggagcaca
     5281 gcctttattt tcgaagtcat gtcaaaactg tatcatgacg aaagcatgcg aaaaaacaga
     5341 aagagctaaa cgatgcgtat caaacggttt ctcctgatag cattattatt gcctgtatca
     5401 gcgcaggctg cccagccctt tgatcaatgg cttaaagatc tgcgccatga agctgcaagc
     5461 aaagggatct caaacaaaac catcgatgtg gcgctcaccg gcattgagcc tattcctcgg
     5521 gttatcgaac ttgaccgcaa acagcccgaa ggccgcatga ccttcaacga atattatcat
     5581 aaggtgatta atcaacagcg catcgaccag gggcgcacta tgtaccgcaa acaccgcgca
     5641 gaattagaac gcgctgctcg gaaatacggc gttcctgcca actatatcgt tgcactctgg
     5701 ggaatcgaaa ccaattacgg ctccaatact ggcggattta aagtcgtgcc agcgctcgcc
     5761 acactcgccc acgatggtcg ccgcgcggaa tttttccgca atgaactgat gtatgccctc
     5821 aaaatccttg atcaaggcca tatttcacca gccgatatga aaggctcatg ggccggggcc
     5881 atgggacaaa atcagttcat gccttccagc ttccatgctt tcgccgttga cggaaacaac
     5941 gatggccgcc gcgacatctg ggcgagcctg cccgatgtat ttgcctccac cgccaattat
     6001 ttaagcaaaa gcggctggaa agcggatcag cgttggggca gggaggtcaa aatcccccaa
     6061 gacttccctg aaagcctcac cggtctgaaa gtcaaaaaga gcctcgatga atggaaaacc
     6121 atggggataa cattaccatc cggagaaaat ctgccgacag cccccggaat tagagcctct
     6181 ttagtcgcac cagatggcct caatggtcgg gttttcttgg tttatgataa ttatcatgtt
     6241 attatgaggt ggaataagtc cacgtatttt gctacatccg tcggattgct agcgaatcag
     6301 gtcgcacaat aaacgctctt aaaacagttt tttaaaaacc aagaagtgaa gatcaccatg
     6361 aatagccgct ttctcacgct cttgattaca atcctatatg ccggactgct ttcaggttgc
     6421 gctgagattg aacttgcctc ccacgttgct aaaaaagccc ctattccagc aacgagcaaa
     6481 agcaaaggta cgtttaaagt aggctcatcc tataaggtcg caggacaatg gtacaaaccg
     6541 cgcgaaacat atagctttga acaaaccggc gtcgcctcgt ggtacggccc taatttccat
     6601 ggcaagaaaa cagccaatgg cgaaacgttt gacatgtacg agatcaccgc tgcacataaa
     6661 actctacaaa tgccttcact catccgcgta accaacctcg aaaatggccg ctcaattatc
     6721 gcccgcgtga atgaccgggg tccgttttca aagaaccgca ttcttgatgt ctccaaacgt
     6781 ggcgccgagc ttctcggctt taaaaatcag ggtacagcgc gggtaaaaat tcaggtcttg
     6841 gccaaagaaa gcagacacgc agccgctatc gccaaacagg gccgttcaac tcgtggaatg
     6901 gaggtggcta tgaacgaacg caactaccag ccggcttccc atcgcttgac tactgtaccg
     6961 tcatctcgcc agcctacacg ccaaccagac gtagaaactc acactgtcgc cgcactccaa
     7021 cctgttgaag ccatacccct taataacgcg catgcatccg ctgctataag acacgccaca
     7081 caacaacaag cccaaagcat atacgtacaa gctgcctctt tcggcgaccc tgtcaaagcc
     7141 aaaagcgttg ctgacacact caaggacttc ggtgatgcac atgttcaacg tgctgttgtc
     7201 aatggtcgta actactttcg cgtacgcatc ggacctctcc aaaacgaacc ccaggccaat
     7261 caggttgtcg ccaggctcgc ctctgccgga cgtgacgatg ccattgtcgt aaccgattaa
     7321 gtaaatattt cccaagcaat tggttgattc tccggcgcga atctacaact ctagaggcat
     7381 gttttttaaa ttcgccctat gaaagtagat ggatcgccat gttgaaatta tgcacccttg
     7441 ccttaagcct tgtaatgttc tgtgggctgc ctgtgctggc tgcgcatgca gcctccatcg
     7501 aaaccaaggc caaacaggcc attgttgtcg actatgagac tggcacagtt ctgctttcaa
     7561 aaaatatgga tgagcaaatg cccacctcat cgatgagcaa ggttatgaca atgtatgccg
     7621 ttttcgacag tatcaaaaac ggccaaattt ccctcaacga caccttgcca gtgagtgaaa
     7681 aagcctggcg catgggtggt tccaaaatgt tcgttgaagt cgataaacgc gtaaaagtcg
     7741 aagatcttat tcgtggcgtc atcgtccagt ccggcaacga cgccaccgtc gttttggcag
     7801 aagggcttgc cggttcagaa aacgcctttg ctaacatgat caacgataaa gctacagggc
     7861 tgggcatgaa aaactcacat ttcgtcaatg ccagtggctg gcctgatcct gaacattact
     7921 caaccgcgcg tgatcttgct acgctgggcc gtgccattat tcacgatttt ccggattatt
     7981 ataaatatta catggaaaag gaattcacct ataacaacat caaacaaacc aaccgcaatc
     8041 cgctgctcta ccgtaatatc ggcgccgatg gcatcaaaac cggccacacc gaagc
//
LOCUS       DAOSWR010000090         8029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5785_length_8029_cov_4.594934, whole genome shotgun sequence.
ACCESSION   DAOSWR010000090 DAOSWR010000000
VERSION     DAOSWR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_8029_cov_4.594934"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PK513_07330"
     CDS             <1..1171
                     /locus_tag="PK513_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOO82297.1"
                     /translation="SVLCACLVEPEKLKGDALKAYNNWRGETVSFEAVDASTASLVVD
                     AVFGTGFKGDLKAAVSDVFGQVRCLVVAVDIPSGVNGDTGAVDAHTLRADCTLTFHRK
                     KLGHVLYPGAGYCGQVHEVDIGITADFESDALENHPDLWLKTFPRKGLGGHKYDNGLV
                     QVYGAPKLTGATRLASEACARIGAGLVAVVAPKGTEDVYRAALAPHILVYDQEDQPER
                     VAVRLYGPGGLGVKPDYKSDVPTVLDADALNDLPAGLSENYVITPHEGEFVKAFPDIV
                     GTKLDKARIAAMQIGAVIVLKGADTVIVHPDGRAVINAHASAVLATAGSGDVLAGMIA
                     GLLAQGMPAFEASCASVWMHGEAALRFGPGLVASDLIDLIPEVLQEELGFCAKLR"
     gene            1202..1286
                     /locus_tag="PK513_07335"
     tRNA            1202..1286
                     /locus_tag="PK513_07335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1236..1238,aa:Leu,seq:tag)
     gene            1309..2766
                     /gene="tig"
                     /locus_tag="PK513_07340"
     CDS             1309..2766
                     /gene="tig"
                     /locus_tag="PK513_07340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO82298.1"
                     /translation="MSAMKVKELKSEGLNHELEITIPANDIEKRVDVKLQEVGKTARM
                     PGFRPGKIPHAMLKQRYGKAILGEVLEAAVNETSQKALQDKKIKPALQPKIEVKSKDF
                     GEGNDLVYIVQVEAMPEFKVADLKGIKLTKMVTTPDDNEVDQALERIAANNQSTKVVE
                     AKRGAKDGETLVIDFHGRTADDNVEHEGMHAHGHHLKLGSGQFIPGFEEQLLGKKAGE
                     KVEVKVSFPENYGAEELAGRDAIFDVDIHEIREPAKTEINDEFAKSLGIDDVKALKEA
                     VKEQLQKELDGHSRMVLKKELLDALDDANKFDVPPGMLEMENTNIIQQVELDRQRNGE
                     DKELSKAEKDEYAQIAERRVRLGLILAEVGNGQNITVADAELHKAVITEAQKFPGQER
                     EVFDYYGKNRHALESLRAPLFEEKVVDYILELAEVKEKSVSQDELLKALDDEEGEAKP
                     KKKAAAKKSSAKKSDGKKKPATQKAAAKKKAAAKK"
     gene            2866..3519
                     /gene="clpP"
                     /locus_tag="PK513_07345"
     CDS             2866..3519
                     /gene="clpP"
                     /locus_tag="PK513_07345"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOO82299.1"
                     /translation="MSTDFNERDPLEVYNNTLVPTVIEQTNRGERAFDIYSRLLKERI
                     IFLTGEVHDYVTSLICAQLLFLESENPKKPISFYINSPGGVVTAGLSVYDTMQYIKPE
                     VHTVCIGQAASMGSLLLAAGAAGHRYSLPNSRIMIHQPSGGARGQATDIEIQAREILR
                     LRERLNEIYVHHTGQKLADVEKAMERDNFMSAKEAKKFGLIDEVVENHVLADDDKKK"
     gene            3610..6030
                     /gene="lon"
                     /locus_tag="PK513_07350"
     CDS             3610..6030
                     /gene="lon"
                     /locus_tag="PK513_07350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOO82300.1"
                     /translation="MLGSSKSSSQRYAVLPLRDIVVFPHMIVPLFVGRDKSVHALEHV
                     MHENKQILLLTQKSPSEDDPSANDLYTTGVVGTILQLLKLPDGTVKVLVEGGKRVKVA
                     GFSENKDFLEAGVEDVVDTTEMNDELEALARAVTTQFEQYVKLNKKIPPEVIVSVNQI
                     EEPTKMADTIASHLALKIEQKQELLEVSTLHERLERIYGFMEGEIGVLQVEKRVRSRV
                     KRQMEKTQREYYLNEQMKAIQKELGDSEDGLDELGELEKQIEEANLPKEAKERAAKEL
                     KKLRQMSPMSAEATVVRNYLDWILAVPWKKRSRVKSDIKLAKKILNADHYGLEKVKER
                     ILEYLAVQQRTKKVKGPILCLIGPPGVGKTSLGQSIARATNRNFVRMSLGGVRDESEI
                     RGHRRTYVGALPGKVIQGMKKAKTVNPLFLLDEIDKLGSDWRGDPSSALLEVLDPEQN
                     STFQDHYLEIDYDLSDVMFVCTSNSLQTMPRPLLDRMEIIHISGYTEDEKLEIAKRHL
                     LKKQMKAAGLKKDEFSISDNGLRDLIRYYTREAGVRSLERELSRLCRKAIKEILMKGK
                     ERISITPRNLKKYAGVKKFKTREIDDKDNVGVVSGLSYSEVGGSILQIEAVNYEGKDG
                     KVSMTGNLKDVMKESITVAEMLIKSRAAAYGIDYEALQKKQIHVHVPEGAVPKDGPSA
                     GAAMVTAIVSCLTGIPISKDIAMTGEVNLRGKVTEIGGLKEKLLGALRGGIKKVLIPE
                     DNVKDLEEIPANVKKELEIVSVGTIDEVLAHALLEAPKPLDSDSSKKIEQIAPESAEN
                     KGEDVIRH"
     gene            6213..6491
                     /locus_tag="PK513_07355"
     CDS             6213..6491
                     /locus_tag="PK513_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOO82301.1"
                     /translation="MNKQDLVSKVADKADISKTKAASAVDAVIDAIKGSLKKGDDVRL
                     VGFGTFSVAKRAATTGRNPRTGEPIKIPASKQPKFKAGKELKEAVNKK"
     gene            complement(6623..6697)
                     /locus_tag="PK513_07360"
     tRNA            complement(6623..6697)
                     /locus_tag="PK513_07360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6663..6665),aa:Val,seq:tac)
     gene            6799..>8029
                     /gene="ispG"
                     /locus_tag="PK513_07365"
                     /gene_synonym="gcpE"
     CDS             6799..>8029
                     /gene="ispG"
                     /locus_tag="PK513_07365"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO82302.1"
                     /translation="MVQIMEKPRHKTHAVKVRGVTVGDDAPVVVQSMTNTDTADVEAT
                     FEQVKDLFLAGSEIVRVTVNTEEAARGVVVLRERLEAAGLDVPLAGCFHYNGHTLLND
                     VPELCEALDKYRINPGNVGFGKKRDAQFESMIEKAVQHNKAVRIGVNWGSLDQELATK
                     LMDENAARAAPWDAEAVMRETLVQSALLSAQRAESVGLRKDQILLSAKVSRVQDLIAV
                     YRALAERSTYALHLGLTEAGMASKGIVATALGEGILLNEGIGNTIRASLTPEPGGDRT
                     LEVRVCKEILQSLGLRSFTPEVAACPGCGRTTSTFFQELAQDIQRHLDESMPVWRKRY
                     PGVENLKVAVMGCIVNGPGESKHADIGISLPGTGESPVAPVFIDGKKVHTLRGDNIAA
                     EFQALVKEYVERRFGTSD"
BASE COUNT         2070 a         1481 c         2412 g         2066 t
ORIGIN      
        1 aagcgtgctg tgtgcgtgtt tggtcgagcc ggaaaagctc aaaggggatg cgctgaaggc
       61 ctataataat tggcgcgggg agacagtttc gtttgaggca gttgatgcaa gtacggcgag
      121 tttggttgtc gatgcggtgt ttggcacggg ttttaagggc gatttaaagg cagctgttag
      181 cgatgttttt ggtcaggtgc gttgcctggt ggtggcggtg gatattcctt ctggcgttaa
      241 tggtgatacc ggtgcagtcg atgcgcatac tttacgggcg gattgtacgc tgacatttca
      301 tcggaagaag ctggggcatg tcctctatcc gggggcaggg tattgcgggc aggttcatga
      361 agttgatatc ggtattacag cagattttga atctgatgcg ctggaaaatc atccggattt
      421 atggctgaaa acgtttccgc gaaaaggtct tggcgggcat aaatatgata atggtctggt
      481 gcaggtctat ggcgctccga aattaacagg ggctacgcgg ctggcgagtg aggcttgtgc
      541 gcggattggc gcggggctgg tggctgtcgt agcgccgaaa ggcacggaag atgtttaccg
      601 ggcggccttg gcgccgcata tattggttta tgatcaggaa gatcagccag agcgcgttgc
      661 ggtgcggctt tacgggcctg gtggtttggg cgtgaagccg gattataaga gcgatgtgcc
      721 aacggtgctg gatgcggatg ccttaaatga tttacctgct ggcttaagtg aaaattacgt
      781 tattactccg catgagggag aatttgtgaa ggcttttcct gatattgtgg gtacgaagct
      841 ggacaaagcg cggatagcgg cgatgcaaat cggcgcggtt atagttctta aaggtgccga
      901 tacggtgatt gtgcatccgg acgggcgggc ggttattaat gctcatgcaa gtgccgtttt
      961 ggcgacggcg ggctctgggg acgtactggc agggatgatt gccggactat tggcgcaggg
     1021 gatgcctgcg tttgaggcct cttgtgccag cgtttggatg catggcgagg cggctttgcg
     1081 gtttgggccg gggctggtgg ccagcgatct aatcgattta attcctgaag ttttgcaaga
     1141 agagcttggt ttttgtgcaa agttgcgcta aaaccgctca gtttttagat ttacccggta
     1201 tgcggctgtg gtgaaattgg tagacacgcc agatttaggt tctggtgctt tcgggcgtgg
     1261 gggttcaagt ccctccagcc gcaccataca ggaagtaaca aatagaaaat gagcgctatg
     1321 aaagttaaag aattaaagtc cgaaggttta aaccacgagc tggaaattac tattccagcc
     1381 aatgatatcg aaaaacgtgt tgatgtaaaa cttcaagaag tgggcaaaac ggcccgtatg
     1441 ccgggttttc gtccaggaaa aattccgcat gccatgttga aacagcgcta tggtaaagcg
     1501 attttgggag aggtgttgga agcggctgtg aatgagacat ctcaaaaagc gcttcaagac
     1561 aagaaaatta aacctgcttt gcagccgaaa attgaagtga agagcaaaga ttttggcgaa
     1621 gggaatgatc ttgtgtacat cgtacaggtt gaggcgatgc ctgaatttaa agtggctgac
     1681 cttaagggga ttaagcttac aaaaatggtt acaacgcccg atgataatga agttgatcaa
     1741 gcgctggagc gtattgcagc gaataatcag tctacgaagg ttgtggaagc taagcgcggt
     1801 gccaaagacg gtgaaacgct ggtgattgat tttcatggac gcacggcgga tgacaatgtt
     1861 gagcatgaag gtatgcatgc gcacgggcat catcttaagc ttggttccgg tcagtttatt
     1921 ccggggtttg aagagcagct tctcggcaaa aaagccggtg aaaaagttga ggtcaaagtt
     1981 tctttccctg aaaattacgg ggcagaagaa ttggccgggc gtgatgcgat tttcgatgtg
     2041 gatatccacg aaattcgtga gcctgctaag acggaaatca atgatgagtt cgctaaatcc
     2101 cttggcatag atgatgtaaa agccttgaaa gaagcggtta aagagcagct tcagaaggaa
     2161 cttgacgggc attcccggat ggttttgaag aaagagcttt tggacgctct ggatgatgcg
     2221 aataaatttg atgttcctcc gggcatgctt gaaatggaga atactaatat tatccagcaa
     2281 gttgaactgg atcgtcagcg taatggtgag gataaagagc tttcaaaggc ggaaaaggat
     2341 gagtatgcgc aaatcgccga gcgccgcgtg cgtttggggc ttattctggc ggaagtcggt
     2401 aatggccaga atattaccgt ggctgatgct gagttgcata aggctgtgat tacggaagcg
     2461 cagaaatttc ccggtcagga acgtgaggtt tttgattatt acggcaagaa ccggcatgca
     2521 cttgaatccc tgcgtgcgcc tttgtttgag gaaaaagttg tcgactatat tcttgagctg
     2581 gctgaggtaa aagaaaagtc cgtgtctcag gatgagcttt tgaaagctct ggatgatgag
     2641 gagggcgaag ccaagccgaa gaaaaaagcg gcggccaaga agtcttcagc gaaaaaaagc
     2701 gatggtaaga aaaagccggc tactcaaaaa gcggcggcga agaaaaaagc tgctgcgaag
     2761 aagtaaactc tgcgcaaatt tatattcaga cttgaacaaa ccgtatctcc tcgggtacgg
     2821 tttgtttgta tttacacttc aacaaccctt ttcggataaa aacttatgag cacagatttc
     2881 aatgaacgcg atcctttaga agtttacaac aatacgctgg tgccgaccgt gatcgagcag
     2941 acaaaccgcg gtgagcgggc gtttgatatt tattcgcgcc tcttgaaaga gcggattatt
     3001 ttcctgaccg gggaggtgca tgattatgtt accagcctga tttgcgcgca gcttttgttt
     3061 ctggaatccg agaatcccaa aaaaccgatt tccttttata ttaattcacc tggcggtgtg
     3121 gtaacggctg gtttgtctgt ttatgacacg atgcaatata taaagcctga ggttcatacg
     3181 gtgtgtattg ggcaggcggc gtcgatgggc tcgttgttgt tggcggccgg tgcggctggg
     3241 catcgttatt ccttgccgaa ttcacgcatt atgatccacc agccatcagg gggtgcgcgg
     3301 gggcaggcta ccgatattga gattcaggcg cgtgagatcc tgcggctgcg tgaacgtttg
     3361 aacgagattt atgtgcatca taccggacaa aagcttgctg acgttgaaaa agcaatggaa
     3421 cgcgataatt ttatgagcgc aaaagaagct aagaaatttg gtttgattga cgaagttgta
     3481 gaaaatcacg ttttggccga tgatgataag aaaaaataaa ggttttgggc gtattgtagt
     3541 ggagtatctt gatttttgat aaaaaggttc catttttctg cttaataatc tgaattttta
     3601 aggaaagcca tgctcggaag ctcaaagtcc tcgtctcaac gttatgctgt attgcctttg
     3661 cgcgatattg tagtgtttcc tcatatgatt gtgccgctgt ttgtagggcg cgataagtct
     3721 gtacatgcac ttgagcatgt gatgcatgaa aataagcaaa tcttgctttt gacacagaaa
     3781 tcaccatctg aggacgatcc ttctgccaat gatctttata ctacgggcgt tgtcgggacg
     3841 attctgcaat tgttgaagtt gcctgacggg actgtaaaag ttctggttga gggtggtaag
     3901 cgggttaaag tcgcgggttt ttctgaaaat aaggactttt tggaagctgg cgtcgaagac
     3961 gttgtagata ctacggaaat gaatgatgag cttgaggcgc ttgcacgcgc ggtgacaacg
     4021 caattcgagc aatatgtgaa gctaaataag aaaattccgc ctgaggtgat tgtttcggtt
     4081 aatcagattg aagagccgac caagatggcc gatacgattg cgtcacatct ggcgctcaaa
     4141 atcgagcaaa agcaggaatt gcttgaggtt tcaacgttgc atgagcggtt agagcgtatt
     4201 tatggcttta tggaaggcga aattggtgtg ttgcaggttg aaaaacgtgt gcgcagccgc
     4261 gtgaagcgcc agatggaaaa aactcagcgt gagtattacc tgaatgagca gatgaaagcg
     4321 attcagaagg agcttggcga cagcgaagac gggctggatg agttgggcga gctggaaaag
     4381 cagatcgaag aagctaattt gccaaaagaa gcgaaagaac gtgcagctaa agaattgaaa
     4441 aaattgcggc aaatgagtcc gatgtcggcg gaggcaaccg tggtgcgcaa ttatctcgac
     4501 tggatcttgg cggtgccgtg gaaaaaacgt agccgtgtga aatccgacat caaactagca
     4561 aagaagattt tgaatgccga ccattatggg ttggagaagg ttaaagagcg cattctcgaa
     4621 tatctagctg tgcagcagcg taccaagaag gttaaaggtc cgattttatg tctgatcggt
     4681 ccgccggggg ttggtaagac ctctttggga caatcaattg cgcgggcgac gaaccggaat
     4741 tttgtgagga tgtctttggg cggtgtgcgt gatgaaagcg agattcgcgg tcaccgtcga
     4801 acttatgtcg gtgctttgcc gggtaaggtg attcaaggta tgaaaaaggc caagacggtg
     4861 aatccgttgt tcttgcttga tgagattgac aagcttggct ctgattggcg cggggacccg
     4921 tcttcggcgt tgttggaggt cttagaccct gagcaaaact caacatttca ggaccattat
     4981 ttagagattg attatgatct gtccgatgtg atgtttgtgt gtacgtcgaa ctcgttgcaa
     5041 accatgcctc gacctttgct ggaccggatg gagatcattc atatttccgg ttacaccgag
     5101 gatgaaaagc ttgaaattgc caagcggcat ttgctgaaaa agcagatgaa agcggcaggt
     5161 ttgaagaagg atgagttttc gattagcgat aatggattgc gtgatttgat tcgttattac
     5221 acccgcgagg ctggcgttcg cagtcttgag cgagaattgt cgcggctttg ccggaaggcc
     5281 attaaggaaa tcctgatgaa aggtaaggag cggatttcca ttacgccgcg taatcttaag
     5341 aaatatgcgg gtgttaagaa atttaaaacc cgcgagattg atgataaaga caatgttggc
     5401 gtagtttcag ggctgtctta ttctgaagtt ggcggttcga ttttgcagat tgaagcggtc
     5461 aattacgaag gcaaagacgg taaagtttcc atgaccggga acctgaaaga tgtgatgaaa
     5521 gaatctatta ctgtggcgga gatgcttatt aagtctcgcg cggcggctta tggcattgat
     5581 tatgaggctt tgcagaaaaa gcagattcac gttcatgtgc cggaaggggc cgtgccgaaa
     5641 gacgggcctt cggctggtgc ggcgatggtg acggcgattg tttcatgtct gaccggcatt
     5701 ccgatcagca aagatattgc gatgaccggc gaggtgaatt tgcgcggcaa ggttaccgag
     5761 attggtggtt tgaaagaaaa actgcttggc gcgctgcgcg ggggcattaa aaaggttctg
     5821 attcccgaag ataatgttaa agatttagaa gagattcctg cgaatgttaa aaaagaactg
     5881 gaaattgtga gtgtaggtac gattgatgaa gtgcttgcgc atgcattatt agaggctcca
     5941 aagccacttg atagtgattc cagtaaaaaa attgagcaaa ttgctccaga aagtgcagaa
     6001 aataagggtg aagacgtaat tcgccattga ttcctgcgcg aaaaaagcct aaaagattcg
     6061 ttacacagtt cgaaaaacct tactggcttt ggcttttaaa gtttttcaaa agtcgatttt
     6121 tttgggattt tttctttagg atgttttttt cttttagaat tcttttcaga aaagtcattt
     6181 aattgcttaa ctttaacctg aaggggatta ttatgaacaa gcaagacctc gtatcaaaag
     6241 tggctgacaa agccgacatc tctaaaacaa aagctgcatc tgctgttgat gctgttatcg
     6301 acgctatcaa aggctctttg aaaaaaggcg atgatgtccg tctcgttggt ttcggcacat
     6361 tctcagttgc caagcgcgct gcaacaactg gccgtaaccc acgcacgggt gagcctatca
     6421 agattccggc ttccaagcag cctaagttta aagctggtaa agagctgaaa gaagctgtga
     6481 acaagaagta agaactgaaa ggttcttgtc ccgttggtgc gcaaaagcgt agttagacga
     6541 acgggatctt ggtaatgctt ctaaagaatt ctaaaagccc tgcaggtttg cggggctttt
     6601 ttgcactttg gagaggaagg aatggtgggc gatgacaggg tcgaactgcc gacatcctcg
     6661 gtgtaaacga ggcgctctac cactgagcta atcgccctta tcaaataagg aatggtatgt
     6721 ttagtaactt aatttgaaaa aatcaatagc tttcatgaaa actttgacct tttgtgtaat
     6781 tcactctaaa taaagcgcat ggtccagatc atggaaaaac cgcgtcataa aacgcatgcc
     6841 gtaaaggttc gtggtgtcac cgttggcgat gatgcgcctg ttgttgtgca atcgatgacg
     6901 aataccgata cagcggatgt tgaggcgacg tttgaacagg tgaaggatct gtttttggct
     6961 gggagtgaaa ttgttcgcgt gacggtgaat accgaagagg ctgcgcgcgg agttgtggtc
     7021 ttgcgggagc ggttggaggc ggcggggctg gatgtgccgc tggccgggtg ttttcattat
     7081 aacggtcata ccttgttgaa cgacgtgcct gagctgtgcg aggcgctgga taagtaccgg
     7141 atcaatccgg gcaatgtcgg ttttggcaag aaacgtgatg cgcagtttga gagtatgatt
     7201 gaaaaggctg tgcagcataa caaagccgtg cggattgggg tgaattgggg ctcgttggat
     7261 caagagttgg cgaccaagtt gatggatgag aatgcggcga gagccgcgcc gtgggacgcc
     7321 gaggctgtga tgcgtgaaac gctggtgcaa tcggcgcttt tgagtgcaca gcgcgctgaa
     7381 agcgttggtt tgcgtaagga tcagatactc ctgtcggcga aggtgagtcg ggtgcaggac
     7441 cttattgcgg tgtatcgggc gttggcggag cgttcgactt acgcgctgca tttggggctg
     7501 accgaggcgg ggatggcgag taaaggcatt gtggcgacag cgctgggtga ggggattttg
     7561 ttgaatgagg ggattggcaa tacgatccgt gcctctttga cgcctgagcc gggtggggat
     7621 cgcacgcttg aggtgcgggt gtgtaaggag attttgcaaa gcttaggctt gcggagcttt
     7681 acgccggagg tggcggcgtg tccgggatgt gggcggacga cctcgacatt ctttcaggag
     7741 ctggcccaag acatacagcg gcatctggat gaatcgatgc cggtatggcg taaacgctat
     7801 ccgggggttg agaatttgaa ggtggcggtg atgggctgca tcgtcaatgg tcccggtgaa
     7861 tccaagcatg cggatattgg tatttcattg cccggcacgg gggaaagccc agtggcaccg
     7921 gtgtttatag acggtaaaaa ggtgcatacg ctgcgcggtg ataatattgc ggcagagttt
     7981 caggcgctgg tcaaagagta tgttgaacgg cggtttggga cgagtgatg
//
LOCUS       DAOSWR010000091         7908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5890_length_7908_cov_5.197886, whole genome shotgun sequence.
ACCESSION   DAOSWR010000091 DAOSWR010000000
VERSION     DAOSWR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5890_length_7908_cov_5.197886"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PK513_07370"
     CDS             <1..1015
                     /locus_tag="PK513_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO82303.1"
                     /translation="SADMAIDLGTANTLVYVKDQGIVLNEPSVVAIEMHYGKKNVLAV
                     GNEAKQMLGRTPGSISATRPLRDGVIADFEVTEAMIKYFIRKVHNRRSFVSPKMVICV
                     PSGSTAVEQRAIIESAESAGARRVELIEEPMAAAIGAGLPVTEPSGSMVVDIGGGTTE
                     VAVISLGGIVYARSVRVGGDKMDEAIIAYIRRVHNLLIGESTAERIKKEIGSACPPAD
                     GEGRRMEIRGRDLMNGVPKEIIVTERQVSEALAEPVGQIIEGVKVALEHTAPELAADI
                     VDKGIVMTGGGSLLTNLDFVLRHATGLAVTLADDPLSCVALGTGHALEEPKILRNVLI
                     GSY"
     gene            1205..2215
                     /locus_tag="PK513_07375"
     CDS             1205..2215
                     /locus_tag="PK513_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO82304.1"
                     /translation="MGNTQNNIPELEERFREPEGWQWHSFVHNGHRLRFGSAFPKDSI
                     PDAVVVCLPGLSEFGEKYFEIARYCLDKNLAFWVLDWRGQGRSDRYFENSQKRHSYGF
                     QNDVEDLQAFIMGYIKHSSVHPDKGRIPLAMLAHSMGANIGLHYIKQHPEVFECAAFS
                     APLFGMKACEKMPKRLPLPLSFILQLFMPYNYAPGEKNWSPSTRPCDGSDAMSSDPVR
                     VDIHRKWFEADPSLQVGGVTYGWVYQAVKSSAYLANKLFAKGIHTPCLMALAGCETLV
                     NNTKARHVARSLPHCELLEFEDAKHEILMERDEFRNAYLEKFYGLIKENIIDRPKSLK
                     PF"
     gene            2260..2496
                     /locus_tag="PK513_07380"
     CDS             2260..2496
                     /locus_tag="PK513_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82305.1"
                     /translation="MEKDDKDVIRLSDRILSALELALEQNDLKISELLTRALEQAMTR
                     NTGGGEFIERRDYPPEIERAMDKLCSLRDQNDGI"
     gene            complement(2493..4574)
                     /gene="ligA"
                     /locus_tag="PK513_07385"
     CDS             complement(2493..4574)
                     /gene="ligA"
                     /locus_tag="PK513_07385"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO82306.1"
                     /translation="MNSLLDLEKLDAQVRHADLVKEIRRHDEAYYQQDAPTVSDAEYD
                     KLRKELEDIETRYPDLSSADSPTQTVGAAPSKGFQKVKHAVPMLSLSNVFSEEEVADF
                     LTRIRRFLGLSEDKPIEIVAEPKIDGLSCSLRYEEGKLVLAATRGDGVEGEDITANVK
                     TIEGIPHYIEAIPTVYEVRGEIYMRRNDFMKLNKQQEEEGKSLFANPRNAAAGSVRQL
                     DSEITKKRPLRFFAYSAFGEISADYDPETQWNILKHIRSCDFDVPSQVRICKNIQEIV
                     ENYRNILEERPNLDYEIDGIVYKVNDLKQQQRLGFVSRSPRWATAHKFPAEQAITRLS
                     AIDIQVGRTGALTPVARLEPITVGGVVVSNATLHNEDEIKRKGIRVGDMVVIQRAGDV
                     IPQVVKVLTEKRKGNPPEYEFPDHCPACGSLAIREGDDVVRRCTGGLICPAQAAQRLK
                     HFVSRLAFDIEGLGSKIIEQFYEEGLIKSPVDIFKLKELNATLENPIREREGWGDLSE
                     KNLFDSIRQRQKIAFNRFIYALGIRQVGEATAKRLAANYGDLKNLKTMMALAQDHESE
                     AYAELLNIEDIGPAVADDLLGFFAEEHNQAVLTDLAMLLEIMPYQAPQISDSPVAGKT
                     VVFTGALTTMTRPEAKARAESLGAKVAGSVSKKTDYVVAGADAGSKLKKAKDLGVEVL
                     SEEEWCALIGS"
     gene            complement(4590..5159)
                     /locus_tag="PK513_07390"
     CDS             complement(4590..5159)
                     /locus_tag="PK513_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82307.1"
                     /translation="MQIMWMLCVLLFPSVAWANAGAPLLLIVNFMAFVYGSVLIVAAE
                     TLIYKVLTKDSWKSALYAIFGVNLLSTIVIGLGIPLVIGLVTALLGFLLGLFWQDAAD
                     VAMFVLGTWIYDGSPYIGESYVTYIATGLWLFVLYILTVYFEAYLIRKLWRKRSFKSP
                     VGALKLNIVTNSVTYAGLATFFIYFFIFN"
     gene            complement(5174..6946)
                     /locus_tag="PK513_07395"
     CDS             complement(5174..6946)
                     /locus_tag="PK513_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOO82308.1"
                     /translation="MLASLRIQNVVLIESLNIEFQPGLCALTGETGAGKSILLDSLGL
                     ALGARADSTLVRKGADQATVIAVFEVPLSHPSLLILHNVNIGIEDGMALSSLRGGEAD
                     EAIQKTEMTGDFGSPRRLPAARDDQIIEIIIRRTLSTDGRSKAYINDQPVSAGLLREI
                     AQSLIEIHGQFDTQGLLNPSTHRKMLDDYAGADNTLAGLWKNWQERKMALADLKSTAE
                     ASRAEEDFLRTALEDLDALEPKAGEEDELAALRERLMHREQVMEGLNAAYEALSGEND
                     PVRSAWATLERIADKIGPQGHEAIGALDRASSEIAEALSEIQRLSADLEQSEHDLQTI
                     DDRLFDLRAQARKHVCSVNDLPAKREELAGRLNAIEHADEALAEAVKQVEAARGSYIN
                     AAQKLSDLRTKTARRLDDLVAKELPPLKLEKARFVTRIEPLGESEWGPGGIDRVRFLV
                     TTNPGSDPGPLEKIASGGEMARFMLALKVVMAQVGSAGSLIFDEVDAGIGGATADAVG
                     ERLARLAAGKQVMVVTHAPQVAARAAHHWIVKKEGAQDIKTTVTALATGQPRREEIAR
                     MLAGAVVTEEARAAAEKLLEAKVA"
     gene            complement(6962..7780)
                     /locus_tag="PK513_07400"
     CDS             complement(6962..7780)
                     /locus_tag="PK513_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOO82309.1"
                     /translation="MHVLHVFGVFLLLGVLSACSSTESSKPQVEESVDALYNKAAAAL
                     DAEQYAEATRYFEEVERQHPYSKWATQAQLMSAFASYEGQNYDEAIIALDRFIELHPG
                     NKDIDYAHYLKALCYYEQISDVARDQFMTEEALKALETLINRFPDSKYARDAALKRDL
                     TYDHLAGKEMEIGRYYLNRGHVNAAINRFRAVVEKYQTTTHTAEALHRLVEAYMTLGL
                     SAEAKQVAAVLGYNYPGSKWYERSFALLDERTRQEIIEERGFVDRTIDTIFKPD"
BASE COUNT         1893 a         2016 c         1970 g         2029 t
ORIGIN      
        1 gtccgccgat atggcgattg atttggggac ggccaatacg ctggtctatg tgaaagatca
       61 gggtattgtg ctcaatgagc cctctgtcgt ggccattgag atgcattatg gtaagaaaaa
      121 tgtgctggcc gtgggcaatg aggccaaaca aatgctgggt cgcacgccgg gcagtatctc
      181 tgcaacgcgc cctttgcgcg acggcgtgat tgccgatttt gaagtcaccg aagcgatgat
      241 caaatatttc attcgcaaag tgcataaccg ccgctccttt gtctctccca aaatggtgat
      301 ttgtgtgcca tccggctcta cggctgtcga acagcgcgcc attattgaat ccgccgaaag
      361 tgctggagct cggcgcgtgg aactcattga agagccaatg gccgccgcaa tcggcgctgg
      421 cctgcccgta acagagcctt cagggtccat ggttgtggat attggaggcg gcaccacaga
      481 agtggccgtg atctctttgg gaggcattgt ctatgctcga tcagtgcgcg tgggcggaga
      541 caagatggat gaggcgatta tcgcctatat ccgccgcgtc cataacctgt tgatcggaga
      601 aagcaccgcc gagcgcatta aaaaggaaat cggcagcgcc tgcccgcccg ctgacgggga
      661 agggcgcagg atggaaatcc gtggccgcga cttgatgaac ggcgtaccaa aggaaattat
      721 cgtcaccgaa cgtcaggttt ccgaagcatt ggccgagccg gtcgggcaaa ttatcgaagg
      781 cgtgaaggtt gctcttgagc ataccgcgcc cgaactggcc gctgacattg tcgataaagg
      841 gatcgttatg accggtggcg gctcgttact caccaatctc gacttcgtcc tgcgtcatgc
      901 caccggcctg gccgtaacct tggccgatga ccctctatcg tgcgtggcgc tgggcacagg
      961 ccacgcgctc gaagagccaa aaatcctgcg taacgtgctg attggatcgt attaacctgt
     1021 gatttgaaac gggtctgtaa atattcttct ctatcttatt tgtttataaa cactctaaac
     1081 attaactctt aaatacttta gcccctatat ttttaagtca caccccttct ttgtgctttc
     1141 attcggactt ctttaatata ttgtagcgta tccttagggt attagaggaa actaatttat
     1201 tttaatgggc aacacgcaaa acaatatacc tgaacttgag gagcgctttc gtgagccgga
     1261 gggctggcaa tggcacagtt ttgttcataa tgggcatcgt ttgcgctttg ggtcggcttt
     1321 cccgaaagac agtattcccg atgctgtggt tgtctgcttg ccgggattaa gcgaatttgg
     1381 tgaaaaatat tttgaaatcg cgcgctattg cctggataaa aatctggcct tttgggtttt
     1441 ggactggaga ggccaaggcc gatcggatcg ctattttgag aactctcaaa aacgccacag
     1501 ctacggcttt caaaacgatg ttgaggattt gcaggccttt attatgggct atataaagca
     1561 ttccagtgtt catcctgata agggccgtat tccactggcc atgttggctc attctatggg
     1621 agccaatatc gggctacatt atataaagca gcatcctgaa gtttttgaat gcgctgcatt
     1681 ttctgcaccc ctttttggta tgaaagcatg cgaaaaaatg cccaagcgtt tgccgctgcc
     1741 tttaagcttc attctacaac tctttatgcc atacaattat gcacccggtg aaaagaactg
     1801 gagtccctct acgcgtccct gcgatggttc tgacgcaatg tctagtgacc cggtgcgcgt
     1861 agatattcat cgcaagtggt ttgaggcaga tccatccttg caagttggcg gcgttacata
     1921 tggctgggtg tatcaagcag ttaaatcgag cgcctatctc gccaataagc tttttgccaa
     1981 aggtatccat acgccttgcc ttatggcatt agcgggctgc gaaacactag tcaataacac
     2041 caaagcacgc catgtcgcgc gcagtcttcc ccactgcgaa cttcttgaat ttgaagacgc
     2101 caagcatgag attttgatgg aaagagatga atttagaaac gcttaccttg aaaaatttta
     2161 cggccttatc aaagaaaata tcattgatcg ccctaagagc ttgaaaccat tttaataagc
     2221 aggcaaagtg gttaaaatta ctcaggagaa aaccaagaaa tggaaaaaga cgataaagac
     2281 gtcatcaggc tgagcgaccg tattctatcc gctttagaac tggctctgga acaaaatgat
     2341 ctgaaaattt cagaactttt aacgcgtgcg cttgaacagg caatgacccg taacacaggc
     2401 ggcggcgagt ttattgagcg ccgggattat cctcccgaga ttgaacgcgc aatggataaa
     2461 ctctgttctc tgcgcgatca aaatgacggc atctaactgc cgatcagtgc gcaccattct
     2521 tcttcggaaa ggacctctac gccgaggtcc tttgcttttt tcagcttcga gcctgcatcc
     2581 gcaccagcca caacgtagtc tgttttcttt gaaacggacc ctgcaacctt tgcaccaagg
     2641 ctttcagcgc gagctttagc ttcaggacgg gtcatggtgg tgagcgcacc agtaaaaact
     2701 acggtctttc ccgcgactgg actatcactg atttgcggtg cttggtaggg cataatttcc
     2761 aatagcatgg ccaaatccgt cagcactgcc tgattatgct cttcagcgaa aaaacctagt
     2821 agatcgtctg ctacggccgg accaatatct tcgatattca gcagctccgc ataggcttcg
     2881 ctttcatggt cctgcgccaa ggccatcatg gttttcaagt ttttcaaatc accataattg
     2941 gcagccagcc ttttggcggt ggcttcacca acctggcgaa tccccagcgc atagataaaa
     3001 cggttaaaag caattttttg gcgctggcga atcgaatcaa acagattttt ttctgacaga
     3061 tctccccagc cttcccgttc gcggattggg ttttcgagcg tcgcgttgag ctctttgagc
     3121 ttaaaaatgt cgaccggtga cttgatcaac ccttcctcat agaattgctc gatgattttg
     3181 ctacccagtc cttcgatgtc aaaggccaga cgcgagacaa aatgtttgag gcgctgggcg
     3241 gcctgagccg gacaaatcag cccccctgtg cagcggcgca caacatcgtc gccttcgcga
     3301 atcgccagtg aaccgcaggc cgggcaatgg tcgggaaatt catattctgg tggattgcct
     3361 ttgcgctttt cggtgagaac tttaacgacc tgcgggatga catcgccggc gcgctggatc
     3421 acaaccatat ccccgacgcg gatacccttg cgtttaatct catcttcgtt gtgcagcgtg
     3481 gcattggaca cgaccacgcc gccgacggtg atcggctcta agcgcgcaac gggggtgagc
     3541 gcacccgtgc ggccaacctg aatatcgatc gcgctgaggc gggttatggc ctgttcggca
     3601 gggaatttgt gcgccgtggc ccagcgcggc gaacgcgaga caaagccgag gcgttgctgc
     3661 tgctttaggt cattaacctt gtaaacaatc ccgtctatct catagtcaag attgggcctt
     3721 tcttctaaga tatttctgta attttctaca atctcctgaa tatttttgca aatccgcacc
     3781 tgcgaaggca catcaaaatc acaacttcga atatgtttta gaatattcca ctgtgtttct
     3841 ggatcatagt cggcactaat ttctccaaaa gcggagtatg caaaaaacct caaaggccgt
     3901 ttttttgtga tttcagaatc aagttggcgc acactgccag cggcagcatt gcgaggatta
     3961 gcaaaaagcg actttccctc ttcttcctgt tgcttattca atttcataaa atcattgcgt
     4021 ctcatataga tttcaccgcg tacttcatat actgtgggaa ttgcttcaat gtaatggggt
     4081 attccttcaa ttgttttgac gttagcggta atatcctcgc cctctacccc atcaccacgg
     4141 gtagcagcta agacaagttt tccctcttca tagcgcaatg agcaggacag gccgtcgatt
     4201 ttgggttcgg caacaatctc gattggttta tcttcgctta agcctaaaaa gcgacggatg
     4261 cgagtgagaa aatcggcgac ttcctcttcg ctaaacacat tggagagcga aagcatcggg
     4321 acggcatgct tcaccttttg gaaaccctta gacggcgcgg caccgaccgt ctgggtgggg
     4381 ctgtcggcac tgctcaaatc cggatatcgg gtttcaatat cttcgagctc tttgcggagc
     4441 ttgtcatact cggcatccga aacggttggc gcgtcctgtt ggtaatatgc ctcgtcatgg
     4501 cggcggattt ctttgaccaa atcagcatgg cgcacttgcg catcgagttt ttcgagatcg
     4561 agaagtgagt tcatatgctt cacgccattt taattaaaaa taaaaaaata aatgaaaaac
     4621 gtggctaatc ctgcatatgt cacgctgttg gttacaatat tcagttttaa agctcctacc
     4681 ggagatttaa atgatctttt tctccacaat ttccttatca aatatgcttc gaaatacact
     4741 gtcagaatat aaagaacgaa cagccataaa cctgttgcta tatacgttac ataagattct
     4801 ccaatatagg ggcttccatc ataaatccat gttcccaata caaacatggc aacatccgca
     4861 gcatcttgcc aaaacagccc caacaagaac cctaataatg ctgttactaa cccaatgaca
     4921 aggggaattc ccagccctat gacaatagtg gaaagaagat tgactccgaa tatagcgtaa
     4981 agagcgcttt tccatgaatc ctttgtcaag actttatata tcagcgtttc agcagccaca
     5041 atcaaaacgc ttccgtatac gaacgccata aaattgacga ttaaaagcaa gggagcgccc
     5101 gcattggccc aagcaacgga ggggaaaaga agtacacata gcatccacat aatctgcatt
     5161 atttattctt tctttaggca actttggctt ctaataattt ctctgcagct gcgcgggctt
     5221 cttcggttac gaccgcgccg gcgagcatgc gggcaatttc ttcgcggcgc ggctgtccgg
     5281 tggccagcgc ggtgacggtg gtttttatat cctgagcgcc ttctttctta acgatccagt
     5341 gatgggcggc gcgcgcggct acttgcgggg cgtgggtgac gaccatgacc tgcttaccgg
     5401 cggccagcct ggccagacgc tcgccaaccg catcggcggt ggcaccgcca atgccggcat
     5461 cgacttcgtc aaagatcaaa cttccggcgc tgccgacctg tgccatcacg accttgagcg
     5521 cgagcataaa gcgcgccatt tctccgcccg atgcgatttt ctccagcggg ccgggatcgc
     5581 ttcctgggtt tgtggtcact aaaaaacgaa cgcgatcaat gccgccaggg ccccattcgg
     5641 attcacccag cggttcaatc cgggtgacaa aacgggcttt ttcgagtttg agagggggca
     5701 attctttagc caccagatca tcgaggcggc gagcggtttt cgtgcgcaga tcgctcagct
     5761 tttgcgcggc gttgatatag ctcccgcgtg cggcttcaac ctgctttacg gcctcagcca
     5821 gtgcttcgtc agcatgttca atcgcattca ggcgcccggc taattcctca cgctttgcgg
     5881 gcaaatcatt gacgctgcat acatgtttgc gagcctgagc gcgcaggtca aagagccgat
     5941 catcaatggt ttgcagatca tgctcacttt gttccagatc cgcagaaaga cgctggattt
     6001 cagataatgc ctcagcgatt tccgatgatg cgcgatcgag cgcgccgata gcttcgtgac
     6061 cctgcgggcc gattttatca gcgatccgct ccaatgtggc ccaagcgctg cgcaccggat
     6121 cgttttctcc gcttagcgct tcgtaggctg cattcaggcc ttccatcacc tgttcacggt
     6181 gcatcaggcg ttcacgcaag gcggcaagtt cgtcttcctc gcctgccttt ggctccagcg
     6241 catcaagatc ttcgagtgcg gtgcgtagaa aatcttcttc ggcgcgtgag gcttcagcag
     6301 ttgatttgag atcggcgaga gccatttttc gctcctgcca gtttttccaa agcccagcta
     6361 acgtgttatc cgcacccgca taatcatcaa gcatttttct atgcgtggag gggttgagca
     6421 gaccttgggt gtcgaactgg ccatgaattt cgatcagact ttgcgcaatt tcacgcagca
     6481 atccggcact tacaggctga tcattaatat aggctttgga gcggccatcg gtggagagcg
     6541 tccggcggat gatgatctct atgatttggt catcgcgagc cgctggtaag cggcgcggcg
     6601 atccaaagtc accggtcatc tctgttttct ggattgcttc gtcggcttcg cctcctcgca
     6661 atgacgatag agccatacca tcctctatcc ctatatttac attatgtaaa attaataaag
     6721 acgggtgaga cagaggcact tcaaaaacag cgatcaccgt cgcctgatct gcgcctttgc
     6781 gcaccaaagt gctatctgca cgcgcaccca gagccagccc taaagaatcc agcaggatgg
     6841 atttgcccgc tcccgtctcg ccggtcaggg cgcacaggcc gggctgaaat tctatattca
     6901 ggctttcaat gaggacgaca ttttgaatac gcagagaggc cagcatgtca gggcacccct
     6961 cttaatccgg cttaaagatc gtatcaatcg tgcgatcaac gaagccgcgc tcttcaataa
     7021 tttcctggcg cgtacgttca tccagcagag caaagctgcg ttcataccac ttgctgcctg
     7081 ggtaattgta cccgagtact gcggcaacct gtttggcctc tgcgcttaag ccgagtgtca
     7141 tatacgcctc aaccaaacgg tgcagcgctt cagcggtgtg ggtggtggtc tggtattttt
     7201 caacgacggc gcggaagcga ttgatcgccg cattcacatg gccgcggttc aagtaatagc
     7261 gcccaatttc catttccttg ccggcgaggt gatcgtaggt caaatcgcgt ttcagggcgg
     7321 catcgcgagc atatttacta tcaggaaatc tattgatgag cgtttcaagt gctttaagcg
     7381 cttcttcggt catgaactgg tcgcgcgcga catcagagat ttgttcgtag tagcacaagg
     7441 ctttgaggta gtgcgcgtag tcaatatcct tattaccggg gtggagttca ataaagcgat
     7501 ccagcgcgat gatcgcctca tcatagtttt ggccttcata agaggcaaag gcgctcatca
     7561 actgggcttg cgtggcccat ttcgaataag gatgctggcg ttcaacctct tcgaagtagc
     7621 gggttgcttc ggcatattgc tccgcatcca atgcggcagc ggctttgtta tataatgcgt
     7681 caaccgattc ctcgacctgt ggtttggagg attcggtcga tgaacaggcc gacaaaacgc
     7741 ccagcaacaa aaagacgccg aaaacgtgca atacatgcat agaaacagtt ttctttataa
     7801 tatccatgtt gttatcaata gcgctcccgg cgcaagaatg gaagagtttc atgacgtttt
     7861 tgaggctaaa aaacggggac agatcgcagt gtatgggaag gtttccgt
//
LOCUS       DAOSWR010000092         7872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5925_length_7872_cov_5.574389, whole genome shotgun sequence.
ACCESSION   DAOSWR010000092 DAOSWR010000000
VERSION     DAOSWR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5925_length_7872_cov_5.574389"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..590)
                     /locus_tag="PK513_07405"
     CDS             complement(84..590)
                     /locus_tag="PK513_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HOO82310.1"
                     /translation="MKTLALTIFAFCIFLTPLQAFAGQPRLMATHGDWEVYVFFEDGN
                     KVCYIASQPKLKQGDYTKRGEPFALITHRPVENTRDVFSYITGYTYKAGSEATVTIDG
                     SDFMLFTQDETAWAPDADTDKKLSQALRDGSKMVVKGTSARGTLTTDTFSLKGSSKAY
                     ERMNSECQ"
     gene            complement(715..1251)
                     /locus_tag="PK513_07410"
     CDS             complement(715..1251)
                     /locus_tag="PK513_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO82311.1"
                     /translation="MRGYFGIGVEGISKEQNVGTIARSAHSFGANFFFTIDPQVDVEL
                     MRVSDTSDAFDHVPYYEYASPAELALPDKCALVGVELLDGAIELPSFRHPARAAYVLG
                     PEMGSLSPALVERCDHIIQIPMRFCVNVGVAGALVMYDRLLSMGRFADRPVRAGGPTE
                     VLPERSTGHRRKVRTPKI"
     gene            complement(1268..2539)
                     /locus_tag="PK513_07415"
     CDS             complement(1268..2539)
                     /locus_tag="PK513_07415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO82312.1"
                     /translation="MAESLDMKVLEYMSIKTTNNEHLNNDETVNEVRQGPKFRPVLHA
                     LTSNPGVEMMNYAAGKENMITLAQGEGDSPTPDFIARAAFTAMQDGHTVYGPTLGQAK
                     LRTALSDYYKRIYELEIPSSRFFVTASGSTAMHLSLAALLDKGDEVVAITPIWKNLIG
                     AIELTQADNVQVSLDYSDEKGWTLDLEKLFGACSDKTKVILVVTPSNPTGWSASQDEM
                     RAILEFARARGIWVLADEVYGRIVYESKHTHLRADSFLDVAEPDDLLLVVNSFSKSWA
                     MTGWRLGWIVGPPAAEDKIRDVALYNNLCPPTFTQYGAIAALEQGEEFLKRQIDLWRS
                     NRDLLVERFEKLGNVHMAYPEATFYAFFKVDGQDDCKELTKRFVDEAGVLLSPGCAFG
                     KTAKGYIRMCFAVSERRLSEALDRMERVLKA"
     gene            complement(2736..4850)
                     /locus_tag="PK513_07420"
     CDS             complement(2736..4850)
                     /locus_tag="PK513_07420"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOO82313.1"
                     /translation="MARLLARMQAEDGLSGVLQVPRKKEMRGSRLGRFQAFVQERTGQ
                     DFGAPGPEAYRALHRWSVKNLEEFHRLVWDFCGILGDRGQIAIKNPDSILEAVFFPDS
                     KISFAENMLARAQTHPDDPAIVSRTAGATGDRVLSWSELCGRVSVWEQALESIGVGEG
                     DRVATYLPHGPEAYVLLIAVAQRGGVFSSVGTEMGAQATATRFKQIRPKVLIAADGYM
                     HMKQAGQPGKAEDRMGLIAELQKNVVSIERTVVVENLSDEPDISNLRDVILADDLLQQ
                     FSVKPLEFVRRDFNQPLAILFSSGSTGKPKCFVHGVGGTLLKHAIEHQLQADVRPGDR
                     VFFHSTTSWMMFNWLASGLAQGATIMIYDGNPAYPEADAQLQFAAEYDCTHLGTAAAI
                     IQDVWAKSGVRAERLDLLALRSLMYTGSVLSDEGFAYIEKAIKPGLSVNGICGGTDFV
                     GCYAAGNVFTPTLAGHLKGPVLGMDIEIWDDEGTVMPVGKVGELVVKSPFASRPLYFW
                     DDEDGSRFRGEYFEYFDCDPPVWRHGDAVKFMSSGQLVIEGRSDTTLNQGGVRIGTQQ
                     LYDALMHPDFEGVIEDALAASFKDAQGGDHTALFVVLQDRVLDEDLKKRIKGVISDSV
                     GRLSTPHEVLAVAYILKTPNGKKAEKPTAQALCGKTVKAGETYGEDPDSGALKVELFK
                     QIGEQLRQKPAYGFADTHKKLA"
     gene            4976..7090
                     /locus_tag="PK513_07425"
     CDS             4976..7090
                     /locus_tag="PK513_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOO82314.1"
                     /translation="MRILFLILSLLFASLSATSAYAQNDESPKVKIRLLPEHANVQPG
                     EELWIGIDQSIAQGWHTYWKNPGDSGTPLNISWTNLDIEAGPLHWPSPHKIPYERLMN
                     YGYENKLVVLQKLKLPENLPTHLPVTLTANIEILVCKETCIPEYGTYNLTLNEPDTRS
                     EDNSAFINKARQNLPQNAGWTASFNQVDQDLVLKLNPPAELLKTINLASIELFPEDWG
                     LISNPVKTQAFIQDGALIIKQTRGERPLKEIKQTSGILAFETNDGVHAAYHFTATKNA
                     AKTIQQETSKTAQSGSLNFITAALFALLGGLILNLMPCVFPVLSIKALSLIKISEKHP
                     DLARLHGLSYTAGVILSFLLIAGVLITLQAGGAQIGWGFQLQNPWIVGTLGYLLFIIG
                     LNLIGFFEFANPFANTGGKLTQNDGLAGSFFTGILATLVATPCTAPFMAGAIGYAFIQ
                     PPIVSLVIFTMLGLGLALPYLALSFVPALQKIMPKPGAWMVIFKQALSFPMFIAALWL
                     LWVLAQQTDAYGITAALLGAISIAFGLWLLRHCPKNKTTLSALRIMAGLSFLGALALL
                     PICARPCAQPMGGGLTQTLISLPYSQETLQSALDSDDPVFTEMTAAWCITCKVNHAVA
                     INIDSTKTLFSKHNIRYIVGDWTNEDPEITKYLRKHGRSGVPLYVYYGPRDPETGRRP
                     DAKILPQVLTPAIVKNFIENNT"
     gene            7111..7719
                     /locus_tag="PK513_07430"
     CDS             7111..7719
                     /locus_tag="PK513_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOO82315.1"
                     /translation="MSKFLAYSLAAIVLAFAALPVNAAVEIGQPAPEIEAITTNGQPF
                     KLSEHKGKIVILEWTNHQCPFVVKHYDSGNMQATQKAAHENGVEWISIVSSAPGRQGY
                     VSTEEANKIVSDVGATITAKILDEDGHIGKVYDAKTTPHMFVIDAEGNIAYAGAIDNQ
                     PSPNPATIEGATNYVSAAIDALEAGQPVEVTQTQPYGCGVKY"
BASE COUNT         2161 a         2453 c         1524 g         1734 t
ORIGIN      
        1 tattaaggaa ttcaagccta cggctgtgta agagccacaa aagcgcattc acatgccatc
       61 tacaagactt tagataacat catctattga cactcactat tcatacgctc atacgctttg
      121 cttgatcctt tcaaactaaa cgtatccgtc gtcagcgtac cgcgcgccga agttccttta
      181 accaccatct tactaccatc acgaagggcc tgcgaaagct ttttatccgt gtctgcatcc
      241 ggcgcccagg ctgtttcatc ttgcgtaaac aacataaaat cactgccgtc aattgtgaca
      301 gttgcctcgc ttccggcttt atacgtataa ccggtaatat agctaaacac atcgcgggta
      361 ttttccacag ggcgatgcgt aatcaacgca aacggctcac cacgcttggt ataatcccct
      421 tgcttcaact tcggctgact ggcgatataa caaaccttat taccatcctc aaaaaacaca
      481 tacacttccc aatcgccatg cgtcgccatc aagcggggct gcccggcaaa agcctgcaaa
      541 ggcgtaagaa aaatacaaaa cgcaaaaatt gtcagggcaa gagttttcat ggtgcttcta
      601 tgtccgttag tcattgaata tatatcagcc atttttctat caaaaaatac cgccacatca
      661 tacagcgcat ttcaaaaatt tccaatcacg ttctgaaaat caagaaaaac gcacctaaat
      721 cttaggtgta cgcaccttcc ggcgatgtcc cgttgacctt tccggtaaaa cctcggttgg
      781 cccgcctgcg cgcaccgggc gatccgcaaa ccggcccatc gaaagcaaac ggtcatacat
      841 caccaaagcc ccggccaccc ctacatttac acaaaatctc atcggaatct ggatgatatg
      901 atcacaccgc tccaccagtg caggagataa actccccatc tccggcccca acacataagc
      961 cgctcgagca ggatggcgaa agctgggaag ctcaatcgcc ccgtccagca attccacccc
     1021 caccaacgcg catttatcag gcaacgccag ttccgccggg ctggcatact cgtaatacgg
     1081 cacatgatcg aacgcatccg acgtatcgga aacccgcatc aattcgacat caacctgcgg
     1141 atcaatcgtg aaaaagaaat tcgcgccaaa ggaatgtgca gaccgggcaa tcgtgccgac
     1201 attttgctct ttgctaatgc cttcaacgcc aatcccgaaa tatccacgca tatcccgaac
     1261 catagcgcta tgccttgagt acgcgttcca tccggtccag cgcctcgctc aagcgccgct
     1321 ctgataccgc aaaacacata cgaatataac ctttagccgt tttcccgaac gcacagcccg
     1381 gcgaaagcaa cacgcccgcc tcatcgacaa aacgcttggt cagctcttta caatcatctt
     1441 gcccgtcaac cttgaaaaag gcataaaatg ttgcctccgg ataggccata tgcacattgc
     1501 caagcttttc aaaccgctca accagcaaat cccggttcga acgccataaa tcaatctgac
     1561 gcttgagaaa ctcttcaccc tgttccagcg ccgcaatcgc accgtactga gtgaaagtcg
     1621 gtgggcacag attattatac agcgccacat cgcgaatctt atcttccgcc gccggtggcc
     1681 ctacaatcca gcccaaacgc cagcccgtca tcgcccagct tttcgaaaag ctgttaacca
     1741 ccagcaaaag atcatccggc tcggccacat ccagaaaact atccgcgcgc aaatgcgtat
     1801 gcttgctttc atagacaatc cgcccataaa cctcatcggc cagcacccag atcccacgcg
     1861 cacgcgcaaa ctccaaaatc gcgcgcattt catcctgcga cgcgctccag cccgttggat
     1921 tcgaaggcgt caccacgagg ataaccttgg tcttatcgct gcaggcgcca aacaatttct
     1981 ccagatccag agtccagcct ttctcgtcgg aataatccag agaaacctgt acattatccg
     2041 cttgagttaa ctcaatcgcc ccgatcaaat tcttccagat cggtgtaatc gccacgacct
     2101 catcgccttt atccagcaaa gccgccaaag acaaatgcat cgccgtcgag cccgacgccg
     2161 tcacaaaaaa acggctcgac ggaatttcca gctcgtaaat ccgcttataa taatccgaca
     2221 aagccgtgcg cagcttggcc tgacccaaag ttgggccgta aaccgtgtgc ccgtcctgca
     2281 ttgctgtaaa agccgcccgc gcaataaaat caggcgtcgg ggaatcaccc tcaccttgcg
     2341 ccagcgtaat catattctcc tttccagccg cataattcat catctcaacg cctggattgg
     2401 acgtcaatgc atgcaacaca gggcgaaatt tcggcccctg tcgaacctca ttcacggttt
     2461 cgtcgttgtt taaatgctcg ttattggtcg ttttaatgct catatattcc aaaactttca
     2521 tgtctaaact ttcagccacg cccgatgcat atacatgcgt tcaaagcgct attcaattaa
     2581 tttaaatggg aaacggggcg tgcgcttatg cactatgggc gaaagtacag cgggcctgac
     2641 ccgccgcagt tgctgcggcc gctgatgctg gagcgataaa cgcgatctgg cggctgttcg
     2701 tattcataaa acgattaaaa ccatgacttc agaaatcatg caagcttttt gtgcgtatca
     2761 gcaaaaccat atgccggttt ctgccgcaac tgctcaccaa tctgcttaaa caattcaacc
     2821 ttcaacgccc cgctatccgg atcttcgcca tacgtctctc cggccttcac agtcttgccg
     2881 cataatgctt gcgccgtagg cttctccgcc ttttttccat tcggcgtttt gagaatataa
     2941 gccacggcaa gaacctcgtg cggcgttgac aagcgcccaa cagaatccga aatcacgccc
     3001 ttgatccgct ttttcaaatc ctcatccagc acccgatcct gcaaaaccac aaacagcgcc
     3061 gtatgatcac ccccttgtgc atctttaaaa ctcgccgcca gcgcatcctc aatcactcct
     3121 tcaaaatcag gatgcatcaa ggcgtcataa agttgttgtg ttccaattcg cacccccccc
     3181 tgattcagcg ttgtatcgga tctgccctca ataaccaact gccccgaaga catgaactta
     3241 accgcatcgc catgtcgcca caccggcgga tcacaatcaa aatactcaaa atactccccg
     3301 cgaaaccgcg aaccatcttc atcgtcccaa aaatacaatg gccgcgaagc aaacggactt
     3361 ttaacaacca gctcacccac cttacccaca ggcatcactg tgccctcatc gtcccagatc
     3421 tcaatatcca tacccaaaac aggccctttt aaatgcccag ccaatgttgg cgtaaagaca
     3481 ttcccggcgg catagcatcc aacaaaatca gtcccgccac atataccgtt cacactaaga
     3541 cccggcttga tcgctttttc tatatacgca aacccctcat cggacaaaac agatccagta
     3601 tacatcaagc ttcgcaatgc taataaatcc aaacgctcgg cgcgcacacc gcttttcgcc
     3661 cacacatcct gaataattgc tgcagccgtg cccaaatgcg tacaatcata ttccgcagca
     3721 aactgaagct gcgcatcagc ctccggatac gccggattac cgtcataaat catgattgtt
     3781 gcgccctgcg caagcccgct ggccagccag ttaaacatca tccagcttgt cgtcgaatgg
     3841 aaaaacacac gatccccagg ccgcacatca gcctgaagct ggtgctcaat cgcatgctta
     3901 agcagcgtac cgccaacccc atgcacaaag catttcggtt tgcccgtaga gcccgaagaa
     3961 aacaaaatcg ccaacggctg attaaaatcc cgtcgtacaa attccaacgg ctttaccgaa
     4021 aattgctgca gcaaatcatc agctaaaatc acatctctca aattcgaaat atccggctca
     4081 tcagataagt tttcaaccac aaccgtacgc tcaatactaa ccacattctt ttgcagttcc
     4141 gcaatcaaac ccatcctgtc ctcagcttta cccggctgcc cggcttgctt catatgcata
     4201 tacccatccg cagcaatcaa cactttcggc cttatctgtt taaatcgtgt cgccgtggcc
     4261 tgcgcgccca tttccgtgcc cacagaagaa aatacgcccc cacgctgcgc caccgctatc
     4321 aataagacgt aagcttccgg tccatgcggc aaataagttg ccacccgatc accttcaccc
     4381 acaccgatac tttctaacgc ctgctcccag acactcaccc gcccgcaaag ctctgaccag
     4441 cttaaaaccc ggtcacccgt cgccccggcg gttcgcgaaa caatcgccgg atcatcgggg
     4501 tgcgtctgcg cacgtgccag catattttcc gcaaaagaaa tttttgaatc cggaaaaaat
     4561 accgcctcta aaatcgaatc cggattttta atggcgattt gcccccgatc acccagtatt
     4621 ccacaaaaat cccacaccaa tcggtgaaat tcctccagat tcttcaccga ccagcgatga
     4681 agagccctgt aagcctccgg ccccggcgcg ccaaaatcct gccctgtgcg ctcttgcacg
     4741 aaagcctgaa accgtccaag ccgggagcct cgcatctctt ttttacgcgg cacttgcaaa
     4801 acgccactta aaccatcctc agcctgcatt ctcgctaata atcttgccaa tatcgtaaac
     4861 atatctctct cactgtaacc tcactcccac aatacccgaa taggcctata gctttaaagc
     4921 aataaatcat tatcatggtt acaggtttta actgataaaa aaggcagctc cctttatgcg
     4981 cattttattt ttaatcttat cgctcttatt cgcaagccta agcgcaacat cagcgtatgc
     5041 acaaaacgat gaaagcccga aggtcaaaat ccgcctgctg ccggaacatg caaacgtgca
     5101 gcccggcgaa gaactctgga taggcataga ccagtctata gcgcagggct ggcataccta
     5161 ctggaaaaat ccgggagatt ccggaacgcc cctaaacatt tcatggacaa acctagatat
     5221 agaagccgga ccgctacact ggccgagccc gcataaaatc ccctatgagc gattaatgaa
     5281 ttacggctac gaaaacaaac tcgtagtgct gcaaaaactc aagttacctg aaaacctccc
     5341 gacgcatctt cccgtcaccc tcaccgcaaa catcgagata ctcgtatgca aggaaacatg
     5401 cattcccgaa tacggcacct ataacctaac gctcaatgaa cctgacacac gttcggaaga
     5461 caacagtgca ttcataaata aagcgcgcca aaacctgccg caaaatgccg gttggacggc
     5521 ctcttttaat caagtcgatc aggatttagt cttaaaacta aacccaccgg cagaactcct
     5581 gaaaaccatc aatcttgcca gcatcgagct tttccccgaa gactggggcc taatcagcaa
     5641 tccggtcaaa acccaggcct tcattcaaga cggcgccctc ataattaaac aaacacgcgg
     5701 cgagcgccct ttaaaagaaa tcaagcaaac ttcaggcata ctcgcctttg aaacaaacga
     5761 cggtgtgcac gctgcctatc atttcacagc caccaaaaat gcagcaaaaa ccatccaaca
     5821 ggaaacatca aaaaccgctc aatccggcag tctaaatttt atcaccgccg cattattcgc
     5881 actactcggc ggcctgattc tcaatttaat gccatgcgtt tttccggtct tgtctatcaa
     5941 agccctaagc ctcataaaaa tatcagaaaa acaccctgac cttgctcgcc tccatggcct
     6001 gtcctatact gcaggcgtaa tcttaagctt cctgctcatt gccggagtac tcatcacctt
     6061 gcaagcgggc ggcgcccaaa tcggctgggg cttccaactc cagaatccat ggattgtcgg
     6121 cacactgggc tatctgctct ttatcattgg cctcaacctg atcggttttt tcgaatttgc
     6181 caacccgttc gccaatactg gtggaaagct cacacaaaat gacgggctag cgggatcctt
     6241 cttcaccggt atattggcaa cattggtcgc cacgccctgt accgcaccct ttatggcggg
     6301 cgccataggc tatgcgttca ttcagccccc catcgtatca ttagtcattt tcacaatgct
     6361 gggattaggc cttgccctgc cctatcttgc gctatctttc gtcccggccc tgcaaaaaat
     6421 catgcccaaa cccggtgcat ggatggtaat ttttaaacaa gctctgtcct tccccatgtt
     6481 tatcgctgcg ctctggctgc tctgggttct ggctcagcaa accgacgctt atggcatcac
     6541 cgctgccctg ctcggcgcaa ttagcatagc ctttggcctg tggctactac gtcactgccc
     6601 caaaaacaaa acaactctct cggccctgcg catcatggca ggcctgtctt tcctcggagc
     6661 attagcgctg ctgcccatct gcgcccgccc ctgcgcccag ccaatgggcg gcggtttaac
     6721 ccagaccctc atcagcctgc cctactcaca agaaaccctg caaagcgcgc tggatagcga
     6781 cgatccagtc ttcacagaaa tgaccgccgc ctggtgcatc acctgtaagg ttaatcacgc
     6841 cgttgcgatc aatattgatt caaccaaaac attgttttcc aagcataaca tccgctacat
     6901 cgtcggcgac tggaccaacg aagacccgga aatcacaaaa tatttacgaa aacatggacg
     6961 cagcggcgta ccgctctacg tctattacgg tccgcgtgat cctgaaacag gccggcgccc
     7021 ggatgcaaaa attttaccgc aggttttaac gcctgcaatc gtaaaaaact ttattgaaaa
     7081 caatacttaa ccctataaaa ggagagaacc atgtcaaaat ttttagccta tagcctggcc
     7141 gccatcgtcc tggcctttgc cgccttgccg gtaaatgctg ccgtagaaat cggccagccc
     7201 gccccggaaa tcgaagccat caccaccaac ggccagccct ttaaactcag cgagcacaaa
     7261 ggcaaaatcg ttattctcga atggaccaac caccaatgcc cgttcgtcgt aaaacactac
     7321 gattccggca atatgcaagc cacacaaaag gcggcccacg aaaatggcgt cgaatggatc
     7381 tccatagtct cttccgcccc cggccgccaa ggatatgtca gcaccgaaga agccaacaaa
     7441 atcgttagcg atgtcggcgc aaccataaca gccaaaatac tggatgaaga cggccacata
     7501 ggcaaagtct acgatgccaa aaccacgccg catatgttcg tcattgatgc agaaggaaac
     7561 atagcttacg caggcgcaat cgacaaccaa ccaagcccta acccggctac aatcgaaggt
     7621 gcaaccaact atgtcagcgc cgccattgac gcccttgaag ccggacaacc cgttgaagtt
     7681 acacaaaccc aaccctatgg ctgcggcgta aaatactaac gcccgcaaaa gtccttccaa
     7741 aagccgcccc tgacaaggcg gctttttgtt aattttcgaa acgctgcgaa gcgcttgtca
     7801 gaatcccata tttgccgtaa gctgatcggc aggttggtca ttcttaaata cactcccgat
     7861 atgttcgcaa aa
//
LOCUS       DAOSWR010000093         7838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5952_length_7838_cov_4.919183, whole genome shotgun sequence.
ACCESSION   DAOSWR010000093 DAOSWR010000000
VERSION     DAOSWR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5952_length_7838_cov_4.919183"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /gene="ftsH"
                     /locus_tag="PK513_07435"
     CDS             <1..1733
                     /gene="ftsH"
                     /locus_tag="PK513_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOO82316.1"
                     /translation="HGVFASSGEEFKVMVPPNENVVDRLQGSAVQIAADKEDPEQISL
                     MSILISLLPAFLIIGVWIMFMKQMQGGKGGGAMGFGKSRAKMLNEKHGRVTFEDVAGI
                     EEAKQELEEVVEFLKDPQKFSRLGGKIPKGALLVGQPGTGKTLIARAVAGEADVPFFT
                     ISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQ
                     TLNQLLVEMDGFESNEGVIILAATNRPDVLDPALLRPGRFDRQIVVPLPDIKGREKIL
                     EVHMKKVPLANNVEAIIIARGTPGFSGADLANLVNEAALLAARRGKRVVGMDELEDAK
                     DKVMMGSERKSMVMSEDEKKLTAYHEAGHALVAMHEPASDPVHKATIVPRGRALGLVM
                     RLPEADRLSMSLEKLKADLSVAMGGRVAEEIIFGHDKVTTGASSDIQQATNMARKMVM
                     EWGFSDKLGTIRYAQDQEEVFLGHSVAQSKNMSDETAATVDSEIRRIVEQGYDKAKQI
                     LTENLDDLHKLAKALIEYELLSGDEIKDLLAGKPILRDSDNEPPEDTTPKSSVPSGGG
                     FASGEAPQGA"
     gene            1777..2043
                     /locus_tag="PK513_07440"
     CDS             1777..2043
                     /locus_tag="PK513_07440"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOO82317.1"
                     /translation="MFKFEKNIGDKDKKARIAAAVVLFAASFFISGVVGNVLILLAVV
                     MVVTSFFGFCPAYTFMGMNTCCRDGSCKVEKADDDQASSDDKDA"
     gene            complement(2068..3018)
                     /locus_tag="PK513_07445"
     CDS             complement(2068..3018)
                     /locus_tag="PK513_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOO82318.1"
                     /translation="MKPQTTTVQFCEISDPGPPKTNLGAIALRENDPVYHLHTGLSPF
                     TLSAPHAGWKVPRHMIDQDGNPLGAPAWWFDPDINEHRHEVCDWGTAELVKRLNKLSP
                     HLSSIASNVSRLVVEKNRAWEYAMTPNSSEYGARLCVPGNHNISKEKLAQRKTIYDTY
                     TRAEEKLVSNIKNTHNGAAHIALHSFSPKWHGHGRDHEIGITYFEDTILSRLIEHAFI
                     ERIGNRAASQYPYNLKDGQFSNKIAAPKLSKKYDTPFIMIEIRNDLLQDPQRLEETTQ
                     LFLQILKDIEKNPLYKHCFSRKQSARTSIPAASTPAFEPI"
     gene            3231..3845
                     /locus_tag="PK513_07450"
     CDS             3231..3845
                     /locus_tag="PK513_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82319.1"
                     /translation="MALDSSGRIVDYFSQCAAGGLFERVFPRKNQAGAEKSPDGGTPA
                     TARDVFEYYRGFFEKGSSVCYGEGGETFLSFSDPLYKWGDTEWLVYSAECLEGGREDL
                     CLTSFNGRQGGHQRLFRIGYEPSQEGGLLIFIGGYDGRDGMYHGNEKLYEISGRSLGQ
                     EVSALYKDLVETEGYWPFNTVLKRIASTKPPEALSGNGPDVLKL"
     gene            3885..5255
                     /gene="glmM"
                     /locus_tag="PK513_07455"
     CDS             3885..5255
                     /gene="glmM"
                     /locus_tag="PK513_07455"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO82320.1"
                     /translation="MTQMQKRKYFGTDGIRGRANSYPMTPGMVQRVAMATAKVLRERH
                     GGLPTDRVVIGKDTRLSCYMIEQAMTAGFLSMGMNVILTGPVPTPGIAMLTRSLRADV
                     GVMISASHNSFEDNGIKLFGPDGYKLDDEIELEIEAALEEDLGEHLALPGDLGKAARL
                     DDAIGRYAESVKRSLPRGTTLEGLKVVVDCANGAAYKVAPQVLWELEAEVISIGAEPD
                     GRNINDGYGATATENLQKAVIEHKADIGVALDGDADRLIMVDETGAKIDGDQLMAALA
                     VSMQEAGELQGSALVATVMSNLGLERFLKERGICLIRTAVGDRYVSETMRAEGFNLGG
                     EQSGHLILSDLNTTGDGLQAAIKICSFLKESGKKASAALHLFTPLPQILRNVRFESTV
                     KPLDADEVKAAIEQAESSLVNEGRVLVRASGTESLIRVMAEGDDPGKVEGVVNDLCAV
                     IEKAVG"
     gene            5379..5891
                     /locus_tag="PK513_07460"
     CDS             5379..5891
                     /locus_tag="PK513_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82321.1"
                     /translation="MRFSEVENYNLSAITKYFSEKYDIDKDKADLISLELKRYLFLVA
                     KNKRQYGMAGIVDEYWHTFILSTVQYHDFCDFLGEGYIHHHPDTDEESASETRDAYKL
                     FFNDYEIEFGHSPLKGIWPSVNDGGRCKNSCKGSPSCKNRCKGTPSCKNNCKGGPHPS
                     MQNDLKGVSA"
     gene            6003..7013
                     /locus_tag="PK513_07465"
     CDS             6003..7013
                     /locus_tag="PK513_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOO82322.1"
                     /translation="MKDISQNSALLPSGFEDLLPPQAEVEYDAIARLMAVFRSFGYAR
                     VKPPMAEFEESLLAPGPGAALTSETFRVMDPVTHRMMGLRSDITAQIARIARSRLSDE
                     ARPLRLTYANDVIRTKASQARTQRQFTQVGCEMIGRNDVASDVEICVVALKGLAALGA
                     CGVTMDFALPRIVDDLFAVTKTRQDDIDGLRHTLAGPVDKALSALDVLALPEAACEYI
                     ARLRAVVSGVEAAIAGLDMSAISLTIDPLETRGFEYHSGLAFTLFATGIRGELGRGGR
                     YDIYEGEQAKESAAGFTLYMDTLRQGLKVQETAEVKELSADADWAEVARLQDEGFIVK
                     RG"
     gene            7121..>7838
                     /locus_tag="PK513_07470"
     CDS             7121..>7838
                     /locus_tag="PK513_07470"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOO82323.1"
                     /translation="MTNVAVIGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLV
                     IDGVTYKLHLLPSGIVRESKISIIGNGVVIDPWALMKEIKAIQEKGVDVNPDKLMIAE
                     NAPLILPVHGALDEAMEATRGSKPIGTTKRGIGPAYEDKIARRAIRVCDLEYPDVMRE
                     KLDRMMLHHNALLKGLGAEEVSADDIFDKLMAVRDEILPYRCVTWRVLDQARAEGKKI
                     LFEGAQGMMLDVDHGTYPYVT"
BASE COUNT         1746 a         1378 c         2585 g         2129 t
ORIGIN      
        1 tgcacggcgt atttgcttca agcggcgaag aattcaaggt gatggtgccg ccgaacgaaa
       61 acgtggtaga ccgccttcag ggtagcgcgg tgcagattgc ggctgacaag gaagatcctg
      121 agcagattag tttgatgagt attttgattt cgctgttgcc tgcattcctg atcatcgggg
      181 tgtggattat gtttatgaag caaatgcagg gcggtaaagg cggcggtgcg atgggatttg
      241 gcaagagccg ggctaagatg ctgaatgaaa agcatggccg tgtgacattc gaggatgtgg
      301 cgggtattga agaggctaag caggagctgg aagaggtcgt tgagtttttg aaggatccgc
      361 agaaatttag ccgtttggga gggaagattc cgaaaggggc cttgctggtc ggtcagccgg
      421 ggacgggtaa aacgctgatt gcgcgggccg tggccggtga ggcggatgtg ccgttcttta
      481 ccatttccgg ttctgatttt gtggagatgt ttgttggcgt tggtgcgagc cgggtgcgcg
      541 atatgttcga gcagggcaag aagaatgcgc cgtgcattat ctttatcgac gagattgatg
      601 cagtggggcg gcaccgcggg gccgggctgg gcggagggaa tgacgagcgc gagcagacct
      661 tgaaccagct tttggttgag atggatgggt ttgagtccaa tgagggcgtg attattctgg
      721 cggcgacgaa ccgtccggat gtgcttgatc ctgcactttt gcgtccgggg cgttttgatc
      781 gtcagattgt ggtgccgctt ccggatatta agggccggga aaagattctt gaagtgcata
      841 tgaagaaagt gccgctggcg aacaatgttg aagccattat tattgcacgc gggacgcccg
      901 gttttagcgg ggcggatttg gcaaatctgg ttaatgaagc ggcgcttttg gcggcgcggc
      961 gcggcaagcg cgttgtgggt atggatgagc tggaagatgc caaggataag gtcatgatgg
     1021 gctctgaacg taagtctatg gtgatgagcg aagatgagaa aaagctgact gcgtatcatg
     1081 aggcgggcca tgcgttggtg gcgatgcatg agccggcgtc cgatccggtg cataaagcaa
     1141 cgattgtgcc gcgcgggcgg gcgctgggtc tggttatgcg tcttcctgaa gctgatcggc
     1201 tatcgatgag ccttgagaag ctgaaggcag atttgtctgt ggcgatgggc gggcgcgttg
     1261 ccgaggagat tattttcgga catgataagg ttacgacggg tgcgagtagc gatattcaac
     1321 aggcgaccaa tatggcgcgc aagatggtta tggaatgggg tttttctgat aagcttggta
     1381 caatccgcta tgcgcaggat caggaagagg tttttcttgg gcattcggtt gcgcaatcga
     1441 aaaatatgtc tgatgagacg gcggcgaccg tggattctga aattcgacgg attgttgagc
     1501 agggctatga caaggccaag cagattttga ctgaaaatct ggatgatttg cataaattgg
     1561 ccaaggcttt gattgaatat gaattgttgt ctggtgatga aatcaaggac ctgcttgccg
     1621 gaaagccgat acttcgtgat agtgataatg aaccgccgga ggatacgaca ccaaaatctt
     1681 ctgtgccatc aggtggaggt tttgccagtg gggaagcgcc acaaggagct tagatgttta
     1741 gatttttata ccgtctatac ataaggggag agtgctatgt ttaaatttga gaaaaatatt
     1801 ggagataagg ataaaaaagc ccggattgct gcggcggttg tgctttttgc agcgagtttc
     1861 tttatttccg gtgttgttgg gaatgtattg attttgctgg cggttgttat ggttgttacc
     1921 agttttttcg gtttttgtcc ggcttatacg tttatgggca tgaatacgtg ctgtcgtgac
     1981 ggttcttgta aggttgaaaa ggctgatgat gatcaggcgt cttctgacga caaggatgct
     2041 tgagtagtcc tttttccgga ttgtccgtta tataggctcg aaagccgggg tgcttgcggc
     2101 gggtattgat gtgcgcgctg attgttttcg agaaaagcag tgtttgtata gcgggttttt
     2161 ctcgatgtct ttgagtattt gtaaaaaaag ctgcgttgtt tcttcaaggc gttgcgggtc
     2221 ttgaagcagg tcgttgcgga tttcgatcat gataaaaggc gtgtcgtatt ttttgctgag
     2281 ttttggcgcg gctattttat tgctgaactg gccgtctttc aggttgtatg gatactgcga
     2341 agcggcgcgg ttgcctattc tttcgataaa ggcgtgttcg attagtctgg acaatattgt
     2401 gtcttcaaaa taggtgatgc cgatttcatg gtcgcggcca tggccgtgcc atttaggtga
     2461 aaagctgtgc agtgctatat gtgctgcgcc attatgggtg tttttgatgt tcgagaccag
     2521 tttttcttca gctcttgtat aggtgtcgta gatggttttg cgctgagcga gcttttcttt
     2581 ggaaatattg tggtttccgg ggacgcagag cctggcaccg tattcagagg aattcggggt
     2641 catggcatat tcccatgcgc ggtttttttc aaccaccagg cgcgatacgt ttgaagcgat
     2701 tgaggacagg tgcgggctga gtttgtttaa gcgtttgacc agttcggcag ttccccagtc
     2761 gcagacttcg tggcgatgtt cattgatgtc aggatcaaac caccatgcgg gcgcaccgag
     2821 tggattgccg tcttgatcga tcatatgccg ggggactttc cagccggcgt ggggcgcgga
     2881 gagcgtgaag gggctgagtc ccgtgtgcag gtggtaaacg ggatcgtttt cgcgcaaggc
     2941 aatggctccg aggtttgttt tcggagggcc ggggtcgctg atttcgcaga attgtaccgt
     3001 cgttgtttgc ggtttcatta tgtcttttgt ttcttttatt acggaaacgg tagagatttt
     3061 tgaaatatat gacaataaaa aagtatattt tgtgcacaaa aatgtgttga ttgtctgtgc
     3121 gcgcgatggg tctgtgtgag cgtgtctgcg aattccgcgc ttgttatcct ccgtatttgc
     3181 cagtacaacg gagcttgtga cataggaata tcttggagta agggtttgtt atggcgctag
     3241 acagtagcgg gcggattgtg gattattttt ctcagtgtgc cgcaggtggt ttgtttgaga
     3301 gagtttttcc gagaaaaaat caggcaggtg cggaaaagag tccggatggc gggacaccgg
     3361 cgacggccag agatgttttt gagtattaca ggggattttt tgaaaagggg tcttcggtgt
     3421 gttacggaga gggtggggag acgtttttaa gcttttcaga ccctctctat aagtggggcg
     3481 atacggagtg gcttgtttac tcggcggagt gtttagaggg tggcagggaa gacctttgtt
     3541 taacgtcttt taatggtcgg cagggtggtc atcagaggct tttcagaatt ggctatgaac
     3601 cttctcaaga aggggggctt ctcattttta tcggaggtta tgacggaaga gatggtatgt
     3661 atcatggaaa tgagaagctc tatgaaattt caggaagatc tttgggtcag gaagtttctg
     3721 cgttatataa agatctcgta gagacggagg ggtattggcc gtttaacacg gtgttgaagc
     3781 gtattgcaag cacgaagccc cctgaggctt taagcggtaa cggtccggat gttctgaagc
     3841 tttgatttat taatttgttc tatggatttt tgagggtgtt ggtgatgacg caaatgcaaa
     3901 aacggaaata ttttggaaca gacggaattc gcgggcgggc caatagctat ccgatgacgc
     3961 cgggcatggt gcagcgggtg gcgatggcga cggcgaaagt gctgcgcgaa cgccatggcg
     4021 ggctgccgac ggaccgggtg gtgatcggca aggatacgcg gttgtcttgt tatatgattg
     4081 agcaagcgat gacggccggg tttttatcga tggggatgaa tgtcatcctt acgggtccgg
     4141 tgccgacgcc ggggattgcg atgttgacgc gatctttacg ggctgatgtc ggggtgatga
     4201 tttcggcttc gcataattcc ttcgaagata acgggatcaa gctgtttggg ccggacgggt
     4261 acaagctgga tgatgagatt gagctggaga ttgaagcggc gctggaagag gatctgggcg
     4321 agcatctcgc gttgcccggc gatttgggga aagcggcgcg tttggatgat gcgatcgggc
     4381 gttatgcgga aagtgtgaag cgctctttac cacgtggaac aacgctggag gggcttaagg
     4441 tcgtggtgga ttgcgccaat ggtgcagcct ataaggtggc gccgcaggtg ctttgggaac
     4501 tggaggccga ggttatatcg attggcgcgg agccggacgg gcgcaatatt aatgatggtt
     4561 atggtgcgac ggcgaccgag aatctgcaga aggcggttat tgagcataaa gcggatattg
     4621 gcgttgcgct ggacggggat gcggaccggc tgattatggt tgatgagaca ggcgcgaaga
     4681 ttgacggcga tcagctgatg gcggcgctgg cggtttcgat gcaggaggcc ggtgagttgc
     4741 aagggagtgc gttggtggcc actgtgatgt cgaatttggg gctggagcgg tttttaaagg
     4801 agcggggaat ttgtctgatc cgcacggcgg ttggggaccg gtacgtgtcc gagacgatgc
     4861 gggcggaagg gtttaatctg ggcggggaac aatccgggca tttgattttg tcggatttga
     4921 atacgacggg tgacgggttg caggcggcga tcaagatttg ttcgttcctg aaagagagtg
     4981 gaaagaaggc gagcgcggcg ctgcatttgt tcacgccgtt gccgcagatt ttgcgcaatg
     5041 tgcggtttga aagcacggtc aagccgctgg acgcggatga ggtcaaggcc gcgattgaac
     5101 aagcggaaag ttcgctggtc aatgaggggc gcgttttggt gcgggccagc gggacggaaa
     5161 gcctgatccg cgtgatggcg gaaggcgatg acccgggcaa ggttgaaggc gtggtcaatg
     5221 atctgtgcgc ggtgattgag aaggcagtgg ggtaaaatta gtcataaatt actttgtttt
     5281 tgataatatg cgttgattgt tgtaatctca agaaaataga ggatagcggt tatagaatag
     5341 gactaaattt ttgttcataa tttttaagga gaattaacat gagattttct gaagttgaaa
     5401 attacaatct atctgcaata actaaatatt tcagtgaaaa atacgatatt gataaagata
     5461 aagcagactt gatatcgtta gaattaaaga gatatctatt tttagttgca aaaaacaaac
     5521 gacagtatgg tatggccgga attgttgatg aatactggca tacttttata ctgtctaccg
     5581 ttcaatatca tgatttttgt gattttttag gggaaggcta tatacatcac catcctgata
     5641 cggatgaaga atctgcaagt gaaacgaggg atgcatacaa actttttttt aatgattatg
     5701 aaatagagtt tggtcacagt ccgttaaagg gtatttggcc ttctgtaaat gatggtggtc
     5761 gttgtaagaa cagttgtaag ggatcccctt catgtaagaa caggtgtaaa ggaacccctt
     5821 catgtaagaa taattgtaaa ggcggtcctc acccatctat gcagaatgat cttaagggtg
     5881 ttagtgccta aagagtttgc atgtggtggt ttatctgatt gcttgggttg tttagttttt
     5941 agatggtagc ttgttttttg attttattga ctttaaacgc tttgcccctt aagcgttagg
     6001 gcatgaaaga tatttcccaa aatagcgcgc ttttgcccag tgggtttgag gatttgcttc
     6061 ccccacaggc ggaggtggaa tatgacgcga ttgcgcggct gatggcggta tttcgcagtt
     6121 ttggttatgc gcgggtgaag ccgccgatgg ctgagtttga ggaaagcttg ctggctccgg
     6181 ggccgggtgc ggccttgacg tctgaaacat tccgggtgat ggaccctgta acgcatcgaa
     6241 tgatggggct gcgcagcgat attacggcgc agatcgcgcg gattgcgcgt tcgcggcttt
     6301 ccgatgaggc acggcctttg cggctgactt atgcgaatga cgtaatccgt acaaaggctt
     6361 ctcaggcgcg cacacagcgc cagtttacgc aggtgggttg tgagatgatc ggccggaacg
     6421 atgtggccag cgatgttgaa atttgtgtgg tagctttgaa gggtttggct gctttggggg
     6481 cttgcggcgt gacgatggat tttgccttgc cgcggattgt ggatgacctt tttgcggtta
     6541 cgaagacgag gcaggatgat attgacgggc tgcgtcatac attggccggg ccggtcgaca
     6601 aggctttgtc ggcgctggat gtattggcgt tgccggaggc ggcgtgcgag tatattgcac
     6661 gtttgcgtgc ggttgtttcc ggagttgaag cggcgatcgc tgggttggat atgagtgcga
     6721 tttctttgac gattgatccg ctcgaaacgc gcgggtttga gtatcatagc gggctggctt
     6781 ttaccttgtt tgcaacgggg attcgtggcg agttgggccg gggtgggcgc tatgatattt
     6841 atgagggtga gcaggcgaag gaaagtgcag ccgggtttac gctgtatatg gacacgttgc
     6901 gccaagggtt gaaagtgcaa gagactgcag aggtcaaaga attatcggcg gatgcggatt
     6961 gggccgaggt ggcccggttg caggatgaag ggtttattgt gaaacgggga taaatggcct
     7021 tgcatgctga tgaaaatcag catctttatg acaggggaga tcctgacttt cgtcaggatg
     7081 cgagggtaga aattttagaa aggtatataa aggtatataa atgacaaatg ttgcggtgat
     7141 tggcgctcag tggggcgatg aaggtaaggg gaagattgtc gattggctct catctcgcgc
     7201 tgatgtggtg gtgcggtttc agggcgggca taatgccggg catacactgg tgattgacgg
     7261 agtgacgtat aagctgcatt tgttgccttc tgggatagtg cgtgaatcga agatttcgat
     7321 tatcggcaat ggcgtggtga ttgatccgtg ggccttgatg aaggagatta aagccattca
     7381 agaaaaaggc gtggatgtga atccagacaa gcttatgatt gcggagaatg cgccgttgat
     7441 tttaccggtg catggggcgc tggatgaagc gatggaagca acgcgcggga gcaaaccgat
     7501 cggtacgacg aagcgcggga ttggcccggc ttatgaagat aaaattgcac gccgggcgat
     7561 ccgggtttgc gatctggagt atcctgatgt gatgcgtgaa aagctggacc ggatgatgtt
     7621 gcatcataat gcgttgctta aaggcctggg tgcggaagag gtgagtgcag acgatatttt
     7681 cgacaagcta atggctgtgc gagatgagat tttgccatac aggtgcgtga cgtggcgggt
     7741 gcttgatcag gcacgggctg agggcaaaaa gattttgttt gagggggcgc aagggatgat
     7801 gctggatgtg gatcacggca catatccgta tgtgactt
//
LOCUS       DAOSWR010000094         7822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5976_length_7822_cov_5.122699, whole genome shotgun sequence.
ACCESSION   DAOSWR010000094 DAOSWR010000000
VERSION     DAOSWR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5976_length_7822_cov_5.122699"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK513_07475"
     CDS             <1..558
                     /locus_tag="PK513_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82324.1"
                     /translation="GWFALAGFSVHTPQILLLLGALAGFLVYNMRSPLRRKACVFLGD
                     AGSLCLGLIIAWYAIKLSMPTARVIEPMGVAWVLALPIWDECAQFYRRVREGRHPFSP
                     DRGHFHHHFIQAGFTPGQSVSMILGIMVLSGFVGIVSLNIGVPLVMLTVVWIIGILAH
                     MAASKDLETYPALISKIFPVRDVDE"
     gene            complement(562..1155)
                     /locus_tag="PK513_07480"
     CDS             complement(562..1155)
                     /locus_tag="PK513_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82325.1"
                     /translation="MFKQGEIRQNLLGCLETALFMPAGVTRFCPSRKSMMKSFLIPLI
                     LLPLTLITMLSAHPSGPLNASSMQILAVIYSLRLFVYLSAFLAFVYTMARTMDRLDDF
                     YRFANANNWLAIPAALLMMPLVILFLNGHYEWAEIYPMMVFITLYSYAYTAFMATHVM
                     RIPMELACFIAIAGMAIHQMSLDVLKWAALGFIQLIA"
     gene            1230..1952
                     /locus_tag="PK513_07485"
     CDS             1230..1952
                     /locus_tag="PK513_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82326.1"
                     /translation="MVLLRKFSLCVFCLPLVLAGCSFFPEFGPASSETVRSEEGRYKQ
                     IEQIDSLSPEEQHARARALVEPDKMVQHNMYVKNADEVRQAQLDKDSSERVVKMERDI
                     GFVQNEFKGLKNMFARDDIASVAGNSGALVSVKGVRLGEHPGKTRLVFDLDGPADFKF
                     NLDNAQKLLVISLPGAGWTAAQEHVYKNNKILQAYAAKAAKAGGSVVVVKLRAPAKVL
                     SSAKLGKNAAGMHRIFLDIALL"
     gene            complement(1949..3262)
                     /locus_tag="PK513_07490"
     CDS             complement(1949..3262)
                     /locus_tag="PK513_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOO82327.1"
                     /translation="MSKTVSPRGKSLSKSTQTIMDQKLWAKILSGMIAGIAVGLILSP
                     QGMGLWEKDWAYAAGEWLALPGIIFLGLTQMVIIPLIICSIILGISESGDLEFLKKLG
                     LRIIPYFILTTAISIIIGLAIVNTVKPGLMIDQNIAQNTIESGATNDRTPGQTFENLT
                     IPERIANIIPANPAKAQVERNMLQIVIAAILVGIALITIPSKTGKPFRELCIAGQVIS
                     MRIISWAMAIAPIAVFGLISNITIRLGFDSLISVGAYAFSVLAGLACILLVYMLIVGI
                     FTRTSPLTFLKNIREVQLLAFSTSSSAVTMPFSIQAAEEKLRVRPEISRFIIPLGATI
                     NMDGTALYQAVAAIFLCQVFGIDLTFNETLMLIITTLGASIGTPATPGVGLVVLATIL
                     TGIGVPPEGIALIIGVDRLLDMCRTAVNVTGDLTASKVMDKWIKT"
     gene            3349..3738
                     /locus_tag="PK513_07495"
     CDS             3349..3738
                     /locus_tag="PK513_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOO82328.1"
                     /translation="MKKLLMMSVVTLAFSVAPALAEPHGGPRDGSGDGKGGAMFQKHD
                     TNGDGFVSQEEFLDHAKQRFDDMDGDGDGKISKEEAQAHHAEMKEKWKEKREERMEKR
                     EERMEKREERMEKRMEKREGAPETSDE"
     gene            3781..4377
                     /locus_tag="PK513_07500"
     CDS             3781..4377
                     /locus_tag="PK513_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO82329.1"
                     /translation="MAKNADQMDDSDESLVARVCEGDHQAFAQLVERHSGLFYSAAYR
                     MCANAEEAEEIVQDAFLKLWEKPQSYDAGKGAKFTTWFYRVVTNLAIDRARRKKTQAG
                     PDVLDFMADQRPLADEVLVQNDREAALETAIQSLPERQKAALNLCFYEGFSNKEAAEI
                     LGIGVKALESLLMRAKTALKDDPLIAGLLEKPAEKKTG"
     gene            4382..4729
                     /locus_tag="PK513_07505"
     CDS             4382..4729
                     /locus_tag="PK513_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82330.1"
                     /translation="MMFDDLDHLLKIRKQPEVPEYLSARIVAAARRSQAVSTPSGIAG
                     WRAWPQMALAAMRVRLSMPQMAYALAGLVVFVVGVELGVQADSVSLFSGLSVGDLAEF
                     MAIDDTFVVAEWV"
     gene            4734..5216
                     /locus_tag="PK513_07510"
     CDS             4734..5216
                     /locus_tag="PK513_07510"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOO82331.1"
                     /translation="MDKKIKIVLVGSVVLNLLLGGMVGGHVYKRWSAHPWHEVKEQLS
                     PESRNLVGRTFQSAFREIKPLGDKARKARVDLVKILSADDFDEEAYDKAAAKMLEIGG
                     EMKAHKVETIKTLAAQLSVEERRKMADRMAQMVGGGHERHVERHRRPKMVAPDKKPQH
                     "
     gene            complement(5261..>7822)
                     /locus_tag="PK513_07515"
     CDS             complement(5261..>7822)
                     /locus_tag="PK513_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO82332.1"
                     /translation="QGHKGEDEETVYELSQGYVDGVHRLVKDFDTLAENAGSDENTVY
                     YNELKHRIRAYRDVIIAAIQHASIDLDMAYEILLKANAHYNAINENFDKLLGKSFKDS
                     SSFFTKLENTFEESLIQSVVIGLFFLLLTFIIILLLSRSITKPILNLTGNIRQITAEK
                     DYDLRVLKETNDELGELADSFNLMLGTVTDAQNSLQKEKAFLSAVLDNMMQGVITIDK
                     TGVIKTFNRWAEKTFGYKAEESVGRNVKTLMPEPYRSAHDGYLENYLKTREAKVIGIG
                     REVEGQTKSGAKFPMSLSVTELEIDDERIFVGLAADISEQKDRERNLKHAKRMADQAN
                     KAKSEFLANMSHELRTPLNSIMGMTRMFTEDADLSEDNRSMAQTVLKSATNLLEIVND
                     ILDISKIEAGSMVLEKTGFDLKDMVANVMEAMAPVASTKGIALSYSFSGEDIPYLVGD
                     SFRLSRVLTNLISNAVKYTDMGEVDITIDSKTLGNNRIEIDCKIKDTGIGIAEDKLKV
                     IFEKFTQADETITRKYGGTGLGLAITKELVKIMGGEIDVESEVGKGSVFWFKIPFKIT
                     DSIDQDKQDRVRQKRRKRKEERVLLSAEKARLLVAEDHLLNQEFIQRLLKRMGITHFD
                     VVENGALALETYEKNSSYDMILMDCHMPELNGYDATRAIRKLEEGTDKRIPIIALTAD
                     AMKGTREKCLESGMDEYITKPIDSNEFKEILAQWIALPDKKGSQGSGGNKDNEKIGDE
                     EEKTETPVNMEFLEEYADTPEEIKEFIQVYIEQSDESLDALEKHCVEGESRDWMETAH
                     KLKGGAGMVGAQVLRDLCAEAQNMLDATKEAREDILKQIKDEYQKVKQYLEKATV"
BASE COUNT         1871 a         1677 c         2108 g         2166 t
ORIGIN      
        1 ggttggtttg cactggcggg atttagtgtt cacacaccgc aaatattgct tctcctgggc
       61 gcattggcgg ggtttttagt ttataatatg cgtagccctt tgcggcgcaa ggcttgcgtg
      121 tttcttggtg atgcgggcag tctgtgtctg gggctgatta ttgcatggta tgctataaag
      181 ctgtctatgc ctacggcgcg ggtgattgag ccgatggggg tggcttgggt tttggctttg
      241 ccgatctggg atgaatgcgc ccaattctat cggcgagtgc gtgaagggcg gcaccctttt
      301 tcaccggaca gggggcattt tcatcatcac tttattcagg ccgggtttac gccggggcaa
      361 tccgtttcga tgattttggg cataatggtg ctgagtggtt ttgtcggtat agtcagtctt
      421 aacatcggtg tgccgctggt catgctgact gttgtgtgga taatcggcat tctggcccat
      481 atggctgctt cgaaagattt ggagacatat cctgcgctca tctctaaaat attccctgtg
      541 cgtgatgttg atgaatagac cttaagcaat taattgaata aaaccgagtg cggcccattt
      601 cagaacatcc aaagacatct gatggatggc catgccggca atcgcgatga agcatgccaa
      661 ttccattggg atgcgcatga catgcgttgc catgaaggct gtgtaggcat aggaatagag
      721 cgtgatgaaa accatcatcg ggtagatttc agcccattca tagtggccgt ttaggaagag
      781 tataactagg ggcatcatca gaagggctgc gggtatggcc agccagttgt tggcgttggc
      841 aaaacggtaa aaatcgtcaa gtctgtccat tgtgcgggcc atggtgtaga caaaggccaa
      901 aaatgcgctg agatagacaa agaggcgcag gctgtagatg acggccagga tttgcattga
      961 tgaggcgttt aaaggcccgg acggatgtgc gctgagcatt gtgattaatg tcagaggtag
     1021 caagatcagt ggaatcagga atgatttcat catgcttttg cggctggggc aaaaacgggt
     1081 tacgcctgcg ggcataaata atgctgtttc gaggcagcct aataaattct gacgaatttc
     1141 accttgcttg aacataatac cctctttatc tttttcttat ctttgtgctt aattattagc
     1201 tgtaaaactt aagaaagcgt taatctgcca tggtgttact tcgtaaattt tccttgtgtg
     1261 ttttttgcct gccgttggtt ttggctggat gttctttttt tcctgaattt gggcctgctt
     1321 cttccgaaac ggtgcgtagt gaggaggggc gttacaagca gattgagcag attgattcgc
     1381 tttctccgga agaacagcat gcgcgggcca gggcactggt tgaaccagac aagatggttc
     1441 aacataatat gtatgtaaaa aatgcggatg aggtcaggca ggcgcaattg gataaagatt
     1501 cgagtgagcg tgttgtaaaa atggagcgtg atattggctt tgtgcaaaat gagtttaagg
     1561 ggctgaaaaa tatgtttgcg cgggatgata ttgcttctgt ggccggtaat tccggcgcac
     1621 tggtcagtgt gaaaggggtg cgtctaggcg agcatcccgg gaagacgcgt cttgtgtttg
     1681 atcttgatgg gccggcggat tttaaattta atctcgataa tgctcagaag ctgttggtta
     1741 taagtttgcc gggtgctggt tggacggctg cacaggagca tgtttataaa aataacaaga
     1801 ttttgcaggc ttatgctgcg aaagccgcga aggcgggagg cagtgtcgtt gtagtgaaac
     1861 ttcgtgctcc ggcaaaggtg ctaagcagtg ctaagctggg taagaatgcg gcaggtatgc
     1921 accggatctt tttggatatt gctcttcttt aagtttttat ccacttatcc atgactttcg
     1981 aagccgtaag gtcgccggtg acatttactg cggtacggca catgtcgagg aggcggtcta
     2041 cgcctatgat cagggcgatg ccttcggggg ggacgccgat accggttaaa attgtggcca
     2101 atacaacaag gccaacaccg ggcgtagcgg gcgttccgat ggaggcgccg agcgtggtga
     2161 taatcagcat gagcgtttca ttgaaggtga gatctatacc gaaaacctga cacaggaaga
     2221 ttgcggctac agcctgatag agcgcagtgc catccatatt gatggtggcg ccgagcggga
     2281 tgatgaaacg ggaaatttcc ggacggactc gcagtttttc ttctgcggcc tggatggaaa
     2341 aaggcatagt gacggcggaa gacgatgtgg aaaaggccag aagctggact tcgcggatgt
     2401 ttttcagaaa agtcagcggg gatgtgcgcg tgaaaattcc aacgatgagc atatagacga
     2461 gcaggataca ggctaatccg gccaaaacgc taaaggcata agcgccgacg ctgataaggg
     2521 aatcaaagcc cagccggatg gtgatgttgc tgatcaggcc gaagacggcg atgggcgcaa
     2581 tggccatggc ccatgagata atcctcatgg atatgacctg cccggcgatg caaagttcac
     2641 ggaatggttt gcccgttttg gacggtatag ttattaaggc gatgccgacc aagattgcgg
     2701 cgatgacgat ttgcagcata ttgcgctcta cttgtgcttt ggccgggttt gcggggatga
     2761 tattggcgat gcgctcaggg atagtaaggt tttcgaaggt ttgacccggg gttcggtcgt
     2821 tggtagcgcc ggattcaata gtgttttggg caatgttctg gtcgatcatc aggccgggtt
     2881 tgacggtatt tacaatagca aggccgatga tgatggaaat tgctgttgtg agtataaaat
     2941 aagggattat gcgcaggccc agttttttca agaattctaa atcgccgctt tctgatattc
     3001 caagaatgat tgagcagata atgagcggga tgatgaccat ctgtgtcagg ccaaggaaaa
     3061 ttatgccggg cagggccagc cattctccgg cggcataggc ccagtctttt tcccacaatc
     3121 ccattccttg tggggaaagg atcagtccga cggcgatgcc ggcgatcatt ccactgagga
     3181 tttttgccca gagtttctgg tccattatgg tttgggtgga ttttgataag gattttccgc
     3241 gcggtgagac tgtcttgctc attaaaatga tcctgatata aagacaaagg gtttagggtt
     3301 tttctgcgta caaagattat tacaacaata acataaggac gaaagacgat gaaaaaactt
     3361 ttgatgatga gtgttgttac gctggctttt tctgtggctc cggctctggc agaaccacat
     3421 ggcgggccgc gcgatggttc cggtgacggt aagggtggcg ctatgtttca gaagcatgac
     3481 accaatggcg atggctttgt ttcgcaagag gaatttctag atcatgccaa gcagcgtttt
     3541 gatgatatgg atggtgatgg tgatggcaaa atctcgaaag aggaagcgca ggctcatcac
     3601 gctgaaatga aagaaaaatg gaaagaaaaa cgcgaagaac gtatggaaaa gcgcgaagaa
     3661 cgtatggaaa agcgcgaaga acgtatggag aaacgtatgg aaaagcgtga aggcgcacct
     3721 gagacttccg acgaataaat tatttgcatc tgcggttttg gcggagcata agactgtttc
     3781 atggcaaaga acgcagatca gatggatgat agcgatgaaa gcctggtggc gcgcgtttgt
     3841 gaaggtgacc atcaggcttt tgcgcaactt gtggagcggc attccggctt gttttatagt
     3901 gcggcatatc ggatgtgcgc caatgcggaa gaagctgagg agattgtgca ggacgcgttt
     3961 ttaaagcttt gggagaaacc gcagagttat gacgcgggga aaggcgcgaa atttacgacc
     4021 tggttttacc gtgttgtaac gaatttggca attgaccgag cgcggcgcaa aaaaacacaa
     4081 gcggggccgg atgttttgga ttttatggcg gatcagaggc cactggctga tgaggttttg
     4141 gtgcaaaatg atcgtgaggc cgctctggag accgcgattc agagtttgcc ggagcggcag
     4201 aaggccgcgc ttaacctttg tttttatgag gggtttagta ataaagaagc ggcggagatt
     4261 ttgggcattg gtgtgaaagc gctggaatca ttgttgatgc gggcgaaaac ggccctgaag
     4321 gacgacccgt tgattgccgg gcttttggaa aaaccggcgg agaaaaaaac aggatagaat
     4381 aatgatgttt gatgatttgg atcacttatt aaaaatacga aagcagccgg aggtgccgga
     4441 atatctcagt gcgcggattg ttgcggcggc gcggcgctct caggcggttt cgactccttc
     4501 gggcattgca ggttggcgcg catggccgca gatggcgctg gcggcaatgc gggtgcgttt
     4561 gagtatgccg caaatggcct atgctttggc cggactggtg gtgtttgtgg tgggtgttga
     4621 gcttggcgtt caggccgata gcgttagtct gtttagcggt ttgagtgttg gtgatctggc
     4681 cgagtttatg gcgattgatg acactttcgt ggtggcggag tgggtgtaaa gacatggaca
     4741 aaaaaataaa aatcgtactg gttggttctg ttgttttgaa tttgttgttg ggcggcatgg
     4801 ttggcggaca tgtctataag cgctggagcg cacatccgtg gcatgaggtt aaagaacagc
     4861 tttcgccgga gagccgtaat ctggtcggac ggactttcca gagcgcgttt cgggaaatta
     4921 agccgctggg ggacaaggcc cgtaaagcgc gtgtcgatct ggtaaagatt ttgtcggcgg
     4981 atgactttga tgaagaggcc tatgataagg cggcggcgaa gatgctcgag atcggcggcg
     5041 agatgaaggc gcataaggtt gaaacaatta aaacgctggc cgcgcaattg tcggtggaag
     5101 agcgccgcaa gatggctgac cggatggcgc aaatggttgg aggcggtcat gagcgtcatg
     5161 ttgagcgcca tcgccgtccg aaaatggttg cgccggataa aaagccgcag cattagaggg
     5221 tattattcat aatcgtcaga tcaagtctca actaatacat ttaaacagtg gccttttcca
     5281 aatattgttt tactttctga tactcgtctt taatctgctt taatatgtct tctcgggctt
     5341 ccttcgttgc gtccagcata ttctgcgcct ccgcacaaag atcacgcaaa acttgtgcac
     5401 cgaccattcc tgccccgcct ttcagcttat gggccgtctc catccagtcc ctactctcgc
     5461 cttctacgca atgtttttcg agagcgtcaa ggctttcatc actttgctca atataaacct
     5521 gtatgaactc ctttatttcc tccggcgtat ccgcatattc ctctaaaaac tccatattaa
     5581 caggagtttc tgttttctcc tcctcatctc cgatcttttc attgtcttta tttcccccag
     5641 aaccttggct gcccttttta tccgggagtg cgatccattg cgccagaatc tccttgaatt
     5701 cattagaatc aatcggtttg gtgatatatt cgtccatgcc gctttcaagg catttttccc
     5761 gcgtgccttt catggcgtcc gctgtcaacg cgataatggg aatgcgcttg tctgtgccct
     5821 cttcgagttt tctgatcgct cgcgttgcat catagccgtt gagttccggc atgtggcagt
     5881 ccatcagaat catgtcatat gatgaattct tttcatacgt ttccagcgcc agagcgccgt
     5941 tctccacgac atcgaagtgc gtaattccca ttcgcttgag cagcctttgg ataaattcct
     6001 gattaagcag atgatcttcc gcgacaagaa gacgggcttt ttccgcagac aaaagtactc
     6061 gctcttcttt tctttttcgc cttttctgac gcactcgatc ttgtttgtcc tgatcaatac
     6121 tgtccgtaat tttgaacgga attttaaacc agaaaacaga cccttttccg acttcacttt
     6181 caacgtcgat ctccccgccc attattttga caagctcttt cgtaatagcc aaccccagac
     6241 cggtgccgcc atatttacgg gtaatcgttt catcggcttg cgtgaatttc tcaaaaatga
     6301 ccttcagctt atcctcagct attccgatac cggtatcttt aattttgcaa tcaatctcaa
     6361 tcctgttgtt gccaagcgtt ttgctgtcaa tcgtgatatc aacttcaccc atatccgtat
     6421 atttaacagc gttgctgatc aggtttgtca agacccgact cagacgaaag gaatcgccaa
     6481 ccagataagg aatatcctca ccggaaaagc tgtaagacag ggcaatgcct tttgtgctgg
     6541 caaccggggc catggcttcc atgacattgg cgaccatgtc tttgagatca aatccggttt
     6601 tctcaagcac catgctgccc gcttcgatct tggaaatatc cagaatatcg ttcacgattt
     6661 ccagcaggtt ggtggcggat ttcagaacgg tctgcgccat tgatctgttg tcctcactca
     6721 gatcagcgtc ctcggtaaac atacgggtca tgcccatgat gctgttaagc ggcgtgcgca
     6781 attcatggct catatttgct aggaactcac ttttagcctt gttggcctga tccgccattc
     6841 ttttggcgtg tttaagattt ctttccctat ctttttgctc cgaaatatcg gcggccaggc
     6901 ccacaaaaat ccgctcatca tcaatttcaa gttccgtaac gctcaaagac atggggaatt
     6961 ttgcgccgct ttttgtctgg ccttccactt cacggccaat gcctatgacc tttgcctccc
     7021 ttgttttcag atagttttca agatatccat cgtgtgcgct gcgatagggc tctggcatca
     7081 aagtttttac attgcgaccg acggattcct ccgccttata tccaaatgtt ttttccgccc
     7141 agcggttaaa cgtttttata acaccggttt tatcgattgt aatcacgccc tgcatcatgt
     7201 tatccagaac ggcgctgaga aatgcctttt ccttctgaag gctgttttgc gcatctgtga
     7261 ccgttcccag catcaggttg aaagaatccg ctagttcgcc caactcgtca tttgtttctt
     7321 tcagcaccct gagatcataa tctttttctg ccgtaatttg tctgatattc cccgtgaggt
     7381 ttaaaatcgg cttcgtaata ctgcgagaca gcaacaggat aattatgaac gtcaaaagaa
     7441 gaaaaaacag accgattaca acagactgaa taaggctttc ttcaaatgta ttttccagtt
     7501 ttgtaaagaa ggatgagctg tccttgaagg attttcctag aagcttgtca aaattttcgt
     7561 tgatggcgtt atagtgagca ttcgctttga gcaaaatttc ataagccata tccagatcaa
     7621 tcgaagcatg ctgaatcgcg gcaataataa catcgcggta agcccgtatt cggtgtttaa
     7681 gttcattata gtagactgta ttttcatcac ttccagcgtt ctccgccaaa gtgtcaaaat
     7741 ctttcaccag cctatgaaca ccatccacat atccttggga aagttcatag acagtttcct
     7801 cgtcttcccc tttatgcccc tg
//
LOCUS       DAOSWR010000095         7818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_5980_length_7818_cov_6.031302, whole genome shotgun sequence.
ACCESSION   DAOSWR010000095 DAOSWR010000000
VERSION     DAOSWR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5980_length_7818_cov_6.031302"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..999
                     /locus_tag="PK513_07520"
     CDS             379..999
                     /locus_tag="PK513_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82333.1"
                     /translation="MYHDNNWNAVQGDELAGFLEQIDQIDGKYRVSSETTQVHWRMLP
                     FYDQVALIRVKDPNWTNPKLNIYYLTDQGNLFRLNGTSPPIHEVNAKAPIKVTEENVL
                     DYLRFFCFFVRGEEGPFYVAETMDDPNMPADMDDTTRSVIEGTIRPATFEGMNEQGHY
                     LCDAVVFYSNALFIANFAVQPTGMIEMLDDDPIAADLPVKVDTPVS"
     gene            1141..1791
                     /locus_tag="PK513_07525"
     CDS             1141..1791
                     /locus_tag="PK513_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOO82334.1"
                     /translation="MIKTHLTLAIAVIALLLGSISNAQAQTSRFYLAGYLGLNTFNDQ
                     EFTESASGNNGDFEIDNTMSFAGALGMRLSQQLRVEGELSYRNAEFSTADIVGAGTFS
                     SGGELKSTLLFANMYYDFDVPWSIQPYIGGGLGYGWHSGEINDGSGLMVNASSDHTGI
                     IWNAAAGIKYRPSTDLAFTAGYRYIDAVSDLEIGSYDVDFDTHEFRLGMEWDLPIR"
     gene            1823..1963
                     /locus_tag="PK513_07530"
     CDS             1823..1963
                     /locus_tag="PK513_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82335.1"
                     /translation="MTKITQTKTLNLALQGGGSYGTFMWGVLDALLEDQQRPPRHFSD
                     AP"
     gene            2153..2287
                     /gene="rpmH"
                     /locus_tag="PK513_07535"
     CDS             2153..2287
                     /gene="rpmH"
                     /locus_tag="PK513_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOO82336.1"
                     /translation="MKRTFQPSNLVRKRRHGFRSRMATKQGRQVLARRRAKGRKRLSA
                     "
     gene            2369..2731
                     /gene="rnpA"
                     /locus_tag="PK513_07540"
     CDS             2369..2731
                     /gene="rnpA"
                     /locus_tag="PK513_07540"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOO82337.1"
                     /translation="MHATKDQQSSLTQIKKRAEFLKIQGKGRKWVSHGLILQTAPNEA
                     GEKRIGYTVSKRVDKSAVRRNRIKRRLRAAAADVLSSGALEGHDYILVGRPQTLIRPY
                     EALCDDLRWCLRKMGLNK"
     gene            2728..2961
                     /gene="yidD"
                     /locus_tag="PK513_07545"
     CDS             2728..2961
                     /gene="yidD"
                     /locus_tag="PK513_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOO82338.1"
                     /translation="MKRILQAMIKVYSAAVSPFLGRNCRFQPTCSAYAHEAIETHGTL
                     KGLWFATRRILKCHPFYKGQAHDPVLPKSQNAD"
     gene            3032..5023
                     /gene="yidC"
                     /locus_tag="PK513_07550"
     CDS             3032..5023
                     /gene="yidC"
                     /locus_tag="PK513_07550"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOO82339.1"
                     /translation="MADPKHLPTLPNNPMNKDQMHPDDMRNMFIFFILAAVLYFSYDS
                     YVLKPQREAIKQRTQIEAEIKQALGPAALEEVKPVPVKPRSEIVTQGGRVKIKNAQMH
                     GSLDLNGGRLDDVALSEYFQTLGKKDNVTVLNPRLTDFPRSVEYGWIASNQNVAVPNK
                     NTNWSIRGNAQLLPGNPVTLVWDNGQGVTFERRLELDEHFMFTVTQRVANNSGRNVTL
                     YPYGLISQKGIPPDFQGMWVSHEGPVGFIGEELFNNSYKDMRKTQKFSSQAQQGWLGF
                     TDKYWLTALIPPQGQNVKYSYSFAGDQKDNDNQGLYQADYLGAALELKPGQSGEIQSR
                     LFVGAKRVFLLQDYETNLKIPQFDLAVDFGWFWFMTKPFFYLLHFLGSTIGNMGVAII
                     IMTILIRGAVFPLTNTSYRSFAKMKKVSPLVMELREKYPDDKQKMQAELIQMYQREGV
                     NPMSGCLPILLQIPIFFALYKTFFVTIEIRHAPFFGWIQDLSAADPTSVFNLFGLIPW
                     NPPAFLMIGVWPCLMLVAMIIQKKLNPPPQDPLQRDMANYMPFLFAFIMSRFASGLVI
                     YWTFSAFIGVIQQMIIMKSLGVPIHLFGETEEEKEMDKAIDKGPALHPLAEMAEDEVE
                     EALGLDDEKDKYDMSGMFDEDHPSKKIKPPKPKKSKKKK"
     gene            5020..5655
                     /gene="yihA"
                     /locus_tag="PK513_07555"
     CDS             5020..5655
                     /gene="yihA"
                     /locus_tag="PK513_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOO82340.1"
                     /translation="MREEFSSEALEEARKLFAGPCDFMLGVAALKQLPPATLPEIAFA
                     GRSNVGKSSLVNALTGRKTLAKTSNTPGRTQQLNYFNLGGRLYMVDMPGYGYAKVSKT
                     QRDAWTQLVFDYLRGRPTLQCVFILIDARHGLKDSDIMLMEMLDETAVQYRIVLTKTD
                     KVKSQELSRISEKITATLKKHAAAFPQVLPTSANKGAGLPEFRAVLSAYAA"
     gene            complement(5657..6403)
                     /locus_tag="PK513_07560"
     CDS             complement(5657..6403)
                     /locus_tag="PK513_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82341.1"
                     /translation="MKTKRERIFMDRMHERGNALIYVLIAIALFAALSFTLSRQTDTS
                     EAGTLSQEKAELYATQLISYAAQARSAIDQMLFTAASDIDQLDFTAPTDAGFDTGTLI
                     HKVYHPQGGGLLPGKIPTEAITQVTNDPEPGWYLGRFNNVEWTALAQGNTAGPGGAEA
                     PWEEVILIAYQINETVCGLINKKINGSTAIPTMTSSIRNTLIDDSLHGGTNVELTTDA
                     PDICPDCANIGSLCVQEGGIYGFYTLLADQ"
     gene            6443..7687
                     /locus_tag="PK513_07565"
     CDS             6443..7687
                     /locus_tag="PK513_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOO82342.1"
                     /translation="MQDIIQSLNAPEYFIAAPVGFILGALVVSIAFLLARQKLQTQNA
                     ELKAQLREREIAFDQAGAALDHRFKATAAEALQKSNEAFLQLAQERLKAAQADGAHDL
                     EKRQNTISELIKPVHKNLEDMAKALEQVKATDTNLREEMRTLNRETARLVGALRDPAA
                     QGRWGEYILEGLLDKSGLIKGVHYETQVSMDSGRQKPDAVINMQDGFKIIIDSKAPIN
                     QFVDRLSEEISEEEYGVLMQNLAKQVKGHVKELGKKSYWENVDSPDFTVLFLPSEHIY
                     SLALRADPSLVEYAAGANIIIASPTLLMSLIRVVGVSWRQAELAKNAAEISERGGDLY
                     KRLLTFTGHLEKVGKNLQGAMKGYDAAVGSLERQVLPAARKFKELQAGTNMAELTEFE
                     TIEETPRPLILTAEDECEKKRA"
BASE COUNT         2114 a         1991 c         1788 g         1925 t
ORIGIN      
        1 ggcagggatt gaaccggcag caggaggcga agaagccgca tgttggggag acgctctaaa
       61 cgcagcgatc aacggctccg tgaattacag ctattcaggc gatccttctg aaagcttgtc
      121 agatgcggtg aattgcggtt cacaaaacct ctaaaaactc cgcttaaaga atttaaaagc
      181 cccttcgttt tcaaaggggc ttttttgtat aggaatccat atttctgaca atatacttga
      241 cctgttttgt gtaattctga atagtaagaa agatataaag cgcgtaaata tttactgacg
      301 ctacaaaggg cttgcacacg ccctaaatat ttgcaaataa tgcattaatt atgaagcgag
      361 taaggataga aactctccat gtatcacgat aacaactgga acgcggttca aggcgatgaa
      421 ctcgctggat tcttagaaca aatagaccag attgatggta aataccgcgt atcttcagaa
      481 acaacgcaag ttcactggcg tatgctgcct ttttatgatc aagtagccct catccgcgtt
      541 aaagacccta actggacaaa tcccaagctg aacatctatt acctgacaga tcagggcaat
      601 ttattccgcc tcaatggtac atctccgcca atccatgaag tgaatgctaa agcgccaatc
      661 aaagtcacag aagaaaatgt cctcgattat ttgcgctttt tctgtttttt tgtgcgcggc
      721 gaggaagggc cgttctatgt tgccgaaacc atggacgacc cgaatatgcc tgccgacatg
      781 gatgatacga cccgctcggt tatcgagggc acaatccgtc cggccacctt cgaaggcatg
      841 aatgagcagg gccattatct atgcgatgcc gttgtgtttt attccaacgc attgttcatc
      901 gccaacttcg ccgtacagcc gaccggcatg atcgaaatgc ttgacgacga tccgattgct
      961 gcggatttac ctgtaaaagt tgatacaccg gtttcttaaa cgaccagaaa ctcacaaaac
     1021 atagatatga aagcgggttt ccttatagtc cgctttttgc tatccttaag ggcgtagcca
     1081 tttgcgctgc agtgctttcc attcttctta tttcaaaatt cccacactgt gagaaacatc
     1141 atgatcaaaa cgcatcttac tctcgctatc gccgtaattg cactgctttt aggcagcatt
     1201 tccaacgcac aggcacaaac aagccgcttt tatctcgcgg gctatcttgg tctcaataca
     1261 tttaacgatc aggaatttac cgaaagcgcc agcggcaata acggcgactt tgagatagac
     1321 aatactatgt cttttgctgg cgctcttggg atgcgcttgt cacaacaact ccgcgttgaa
     1381 ggggagctat cctaccgtaa cgctgaattt tcgacagccg acatcgtcgg tgccggtaca
     1441 ttcagctccg gcggcgaact caaaagcaca ttgttgttcg ccaatatgta ctacgatttc
     1501 gatgtgcctt ggtcgatcca gccctatatt ggcggtggtc tcggttatgg ttggcactct
     1561 ggtgaaatca atgatggctc cggcttaatg gtaaatgcct catccgatca taccgggatt
     1621 atctggaatg ctgcggctgg aatcaaatac cgaccaagca cagatctggc tttcaccgcc
     1681 gggtaccgtt atatcgatgc tgtatcggat ttggaaatcg gtagctatga tgtcgatttt
     1741 gatacccacg aattccgcct cggtatggaa tgggacttgc caatccgcta gggtttaaac
     1801 tgtaagtaag ccctcttaaa aaatgacaaa aatcacgcaa accaaaaccc ttaacctcgc
     1861 tcttcagggc ggcgggtcgt acggcacctt tatgtggggc gtactcgatg ccctattgga
     1921 agaccagcag cggccacccc ggcacttttc cgatgcgcca taagccccac agcgacaaaa
     1981 acaagcagga aaaatacaaa gctgtaaatc atttgcccaa gttcttacaa aagaaccgct
     2041 tgtaaagaaa ttcccccgaa tccgttgaca caggggcaaa aataagggta taaccctcac
     2101 acttttaacg aattcaatgg atttgaggcg cctgacaccg ggcgaaagta agatgaaacg
     2161 tacattccag cccagtaacc tagttcgcaa acgccgtcat ggcttccgct cacgtatggc
     2221 tacaaagcag ggccgtcagg ttctggcccg ccgccgtgca aaaggccgta aacgcctgtc
     2281 agcttagttt taagcctgta ttttgtttga aggcgcaaat tttaaggttt tgcgcctttt
     2341 tctatagtta tttgctatac tcgcacccat gcatgcaact aaagatcaac aatcctcgct
     2401 tacacagatt aaaaagcgcg ccgaattcct gaaaattcag ggaaaaggcc gcaaatgggt
     2461 ttcccacggt ctcattcttc aaactgcacc caatgaagcg ggcgaaaaac gtataggcta
     2521 tacggtttcc aaacgtgtcg ataaatccgc tgtgcggcgc aaccgtatca aacgccgcct
     2581 gcgtgccgcc gccgccgatg tcctttcctc tggcgcattg gagggccacg attatatcct
     2641 ggtgggacgc ccgcaaaccc ttatacgccc ttatgaagcg ttgtgtgatg atttgcgctg
     2701 gtgcctgcgc aaaatgggct taaacaaatg aaacgcatct tgcaagccat gattaaggtt
     2761 tatagcgctg ccgtttcacc atttttaggc cgcaattgtc gtttccagcc gacatgctct
     2821 gcctatgcac atgaggccat tgaaacccac ggtacgctca aaggtttgtg gtttgccacc
     2881 cgtcgtattc tcaaatgcca cccgttttac aaaggacagg ctcacgaccc cgtgctgcca
     2941 aaatcccaaa acgctgattg atcacatctt tcagatcggc tataaacgct tccaaaccca
     3001 aattacacga cataaagcaa agcaaacaca aatggccgat ccaaaacacc tgccaactct
     3061 gcctaataac ccgatgaaca aggatcaaat gcatcctgac gacatgcgca atatgttcat
     3121 cttttttatt ctcgcagcgg tgctttattt ttcctacgac agttatgtac tcaagcctca
     3181 gcgcgaagcg attaaacagc gcacacagat agaagctgaa atcaaacagg ctctgggccc
     3241 ggccgcgctg gaagaagtca aacccgtacc agtcaaacca cgttcggaaa tcgttaccca
     3301 aggtggccgg gttaaaatta aaaatgcgca gatgcatggt tcactggatt taaacggtgg
     3361 gcgccttgat gatgtggcct taagcgaata ttttcagacg ctgggtaaaa aggataatgt
     3421 cacggtcctc aacccccgcc tgacagattt cccgcgcagt gttgaatatg gctggatagc
     3481 cagcaaccag aatgtggccg taccaaacaa aaatacaaac tggagcatac gcggcaacgc
     3541 gcagttgtta ccgggcaacc cagtaacgct tgtttgggat aacggccaag gcgtgacgtt
     3601 tgaacgccgc cttgagttgg atgaacattt tatgtttacc gtaacgcagc gtgtggcgaa
     3661 caattccggg cgtaatgtta cgctttatcc ctacggatta atttcacaaa aaggcatccc
     3721 gccggatttt cagggcatgt gggtttcaca tgaaggcccg gtcggtttta ttggtgaaga
     3781 gctgttcaac aatagctata aagatatgcg taaaactcaa aaattctcat ctcaagccca
     3841 gcagggctgg cttggtttta ctgataaata ttggctaacg gctctgatcc cgccacaagg
     3901 gcaaaatgtt aaatacagct atagctttgc aggcgatcaa aaagacaacg acaaccaagg
     3961 gctctatcaa gctgattatc ttggcgcggc attagagtta aaacccggcc agagcggtga
     4021 aatccaaagc cgcttatttg tcggagccaa acgcgtcttt ttgctccagg attatgaaac
     4081 aaatcttaag attccacaat ttgatctggc cgttgatttt ggctggttct ggttcatgac
     4141 caaaccgttt ttctatctgc tgcatttttt aggcagtact atcggaaaca tgggcgtggc
     4201 gattattatc atgacgattc tcattcgcgg tgccgttttc ccactcacaa acacgtctta
     4261 ccgctctttt gccaaaatga agaaagtctc gccgctggtg atggagttac gtgaaaaata
     4321 tccagacgac aaacaaaaaa tgcaagccga actcatccag atgtaccagc gcgaaggcgt
     4381 taatccgatg tcaggctgct tgccgatttt gctgcaaatt ccgattttct tcgcgctcta
     4441 taagacattc tttgttacga ttgaaattcg ccatgcaccg ttttttggct ggattcaaga
     4501 tctctcggct gcagacccaa ccagtgtatt taatctgttc ggactcatcc cgtggaatcc
     4561 gcccgccttc ttgatgattg gcgtctggcc gtgtttgatg ctggtggcga tgatcatcca
     4621 gaagaaactc aaccccccac cgcaagaccc gctccagcgt gatatggcga actatatgcc
     4681 cttcctgttt gcttttatca tgtcacgatt tgcttccggg ctggtgatat actggacatt
     4741 ttccgccttc atcggcgtga tccagcagat gatcattatg aaatcccttg gcgtacccat
     4801 ccacctattt ggcgaaaccg aagaagaaaa agagatggat aaagccattg ataaaggtcc
     4861 ggcgctgcac cctctggccg aaatggctga agatgaagtt gaggaagctt tgggtctaga
     4921 cgatgaaaaa gacaaatatg atatgtcggg aatgtttgat gaagaccatc cctctaaaaa
     4981 gataaaaccg cccaagccta agaaaagcaa aaagaagaaa tgagagaaga attttcgtct
     5041 gaagctttag aagaggcccg caaactgttt gcgggccctt gcgattttat gctcggcgtt
     5101 gcagcgttga aacaacttcc gcctgctact ttgccggaaa tcgctttcgc tggccgttcg
     5161 aatgtcggta aatccagtct tgttaacgcg ctcacaggcc gtaaaacact ggcgaaaacc
     5221 tcaaatacac cgggccgcac ccagcaactc aactacttta atctaggcgg gcgtctctat
     5281 atggtcgaca tgcctggcta tggctatgcc aaagtgtcta aaacccagcg cgatgcatgg
     5341 acacagcttg tctttgatta cttgcgtggt cgcccgacgt tgcaatgcgt ctttatcctg
     5401 attgatgcgc gccatggcct caaagacagc gatataatgc tgatggaaat gctcgacgaa
     5461 acggctgttc aataccgcat cgttctgaca aaaaccgata aagtgaaatc gcaggaactt
     5521 tctagaattt cagaaaaaat tacagccact cttaaaaaac acgctgccgc ttttccgcaa
     5581 gtgctaccta ccagcgccaa taaaggcgca ggcctacccg aattccgcgc cgtgctgagc
     5641 gcctatgcgg cgtaggctac tgatctgcca gcagggtata aaacccgtaa atgccaccct
     5701 cttgcacaca caatgagccg atatttgcgc aatccgggca aatatcgggc gcgtccgtgg
     5761 tcagttcgac attcgtaccg ccatgcaagc tatcatcaat cagcgtatta cgaattgaac
     5821 tcgtcattgt cgggatagcc gtagagccgt taattttctt attaatcaac ccgcacacgg
     5881 tttcatttat ctgataagca atcaaaataa cctcttccca cggcgcttcc gctccaccag
     5941 gacctgcggt attaccctgc gctaaagctg tccactcaac attgttaaac cgtcccagat
     6001 accatcccgg ctccgggtcg ttggtaactt gcgtaatggc ttcggttgga attttacccg
     6061 gcagcaatcc acccccttgc gggtgataaa ccttatgaat caacgtgccg gtgtcaaatc
     6121 ctgcatcggt tggggccgta aaatccaact gatcaatatc gctggccgcc gtaaacaaca
     6181 tctgatcaat ggccgatctg gcttgcgcgg cataggaaat caactgcgtt gcgtagagct
     6241 ccgccttttc ctgactgagc gtcccagcct ccgatgtatc ggtttgccgt gaaagcgtaa
     6301 agctgagcgc cgcgaacagc gcaatcgcga tcagcacata aatcaaggca ttaccacgct
     6361 catgcatcct atccataaaa attcgttctc tttttgtttt catttctttt atccggtcag
     6421 aatcaatgct acgcttgtca ttatgcaaga tattatacag tctttaaacg cccctgaata
     6481 ttttattgcc gcccccgttg gttttatact gggggcgctc gttgtcagca ttgcattttt
     6541 gctggctcgt caaaagctcc agactcaaaa tgcggagttg aaagcccagc tccgtgagcg
     6601 cgaaatcgcc ttcgatcagg ccggcgctgc gcttgatcac cgctttaaag ccaccgccgc
     6661 cgaagccctg caaaaaagca atgaagcgtt cctgcaactg gcgcaagaac gcctgaaagc
     6721 cgcccaggcc gatggcgcgc acgatctcga aaaacgacag aacacaattt ctgagctgat
     6781 taaacccgtc cataaaaatc ttgaagacat ggccaaggcc cttgaacagg tcaaagccac
     6841 cgataccaat ttacgcgagg aaatgcgtac gctgaatcgc gaaaccgcgc gcctcgttgg
     6901 cgcgttgcgc gatccggcag cgcaagggcg ctggggtgaa tacattctcg aaggcctgct
     6961 cgataaatcc gggctgatca aaggcgtaca ctatgaaact caggtctcta tggacagcgg
     7021 cagacaaaaa cctgatgcgg tcataaatat gcaggatggt ttcaaaatca tcatcgattc
     7081 caaagcaccc attaatcagt ttgtagatcg cctttccgaa gaaataagcg aagaagaata
     7141 cggcgtcttg atgcaaaatc ttgcaaaaca ggtaaagggg catgtgaaag agcttgggaa
     7201 aaaatcctat tgggaaaatg ttgacagtcc tgatttcact gtactgtttc tcccttccga
     7261 acatatctac tctctagccc tgcgcgccga cccgtctctg gttgaatacg ccgccggtgc
     7321 caatatcatt attgcttccc ccactttgct gatgagcttg atccgcgttg taggcgtaag
     7381 ctggcgtcag gctgagctgg ccaaaaacgc cgctgaaatt tccgagcgcg gcggtgatct
     7441 ctataaacgt ctgctcacct ttaccggtca tctcgaaaaa gtcggcaaga atttgcaagg
     7501 cgcaatgaaa ggctatgatg ccgctgttgg ctctctggag cgccaggttt tgcccgcagc
     7561 gcgtaaattt aaagaattac aggccggtac caacatggcc gagttaaccg aatttgaaac
     7621 gattgaggaa accccgcgcc ctctcattct cactgccgag gatgaatgcg agaaaaaacg
     7681 agcctaagcc tctgtctgga gctagctcag ggttatcggg agtattatcg tccatagtgt
     7741 caaaacacaa aaaaatattt gtctagggtc cggactcaca gcttaaatcc agaaacataa
     7801 gagggaagca aggcatag
//
LOCUS       DAOSWR010000096         7757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6040_length_7757_cov_5.651779, whole genome shotgun sequence.
ACCESSION   DAOSWR010000096 DAOSWR010000000
VERSION     DAOSWR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6040_length_7757_cov_5.651779"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="rfaE2"
                     /locus_tag="PK513_07570"
     CDS             <1..571
                     /gene="rfaE2"
                     /locus_tag="PK513_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOO82343.1"
                     /translation="DHGDVMARTSAGAQKQDRARKGDVLEHSDHGWEEATEHVRRWKA
                     RGLKVGFTNGCFDILHYGHVSYLNNARDHCDRLIVGLNKDSSVCALKGPQRPVHDEES
                     RAGVLAALGSVDMVVLFGADTDGDDNTAIKLLEALKPDIYFKGGDYTEDQIPESPTVR
                     GYGGEVNVMPIYEGHSTTGSIAKINNEAA"
     gene            complement(653..2257)
                     /gene="guaA"
                     /locus_tag="PK513_07575"
     CDS             complement(653..2257)
                     /gene="guaA"
                     /locus_tag="PK513_07575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOO82344.1"
                     /translation="MSAENVTVPKNASETNKTHDHILILDFGSQVTQLIARRVRESGV
                     YCEIWPFNHADDDRIKSFNPKGIILSGGPCSVLDEEAPAAPEIIFTLGVPVFGICYGQ
                     QTMVKQLGGRVAPGEGAGEFGRAHVEVTDHSDLTQGVWDAGAREQVWMSHGDHVADLP
                     EGFHIVAKSEGAPFAVIANKDKKFYGVQFHPEVVHTPHGRDLFKNFTHNVAGCAGDWT
                     MQNFREESIAKIREQVGDKKVICGLSGGVDSSVTAILLHEAIGDQLTCIYVDTGLMRA
                     GESEQVVALFREHYNIPLIHEEAEDLFLGKLDGVSDPETKRKIIGGLFIDVFDKCAKE
                     VGDAAFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNLKLVEPMRELFKDE
                     VRNLGRALGMPEDFIRRHPFPGPGLAIRMPGEITRGKLEILRKADTVYLDEIKKAGLY
                     DEIWQAFAVLLPVRSVGVMGDGRTYDYVCALRAVTSTDGMTADYYPFEHEFLSRVSTR
                     IINEVAGINRVTYDITSKPPGTIEWE"
     gene            2331..3173
                     /gene="metF"
                     /locus_tag="PK513_07580"
     CDS             2331..3173
                     /gene="metF"
                     /locus_tag="PK513_07580"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOO82345.1"
                     /translation="MSAPTISFEFFPPKTEKASEALWEAVADLAALGPKFMTVTYGAG
                     GSTRDGTVDTIAKMKEDTGLPIGSHLTFINTPKDQLHEFTSALWAAGIRHIVALRGDM
                     PDDLQWPLDEDGEYFQYTSDFVEGLKGWHDFEISVGCYPEKHPDALDMSADIAALKLK
                     CEAGADRAITQFFFDNDVYYNFRDECAAAGIETPIVPGLLPIHDFKSMCNFAKKCQAS
                     VPAGLHEKFAGLEDKPEEAQKVAADLLILQAQNLAQNGVEHLHFYTLNKSMITQEVVQ
                     CLNN"
     gene            3158..3550
                     /locus_tag="PK513_07585"
     CDS             3158..3550
                     /locus_tag="PK513_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82346.1"
                     /translation="MPQQLRQGKKPKLTILISILLTTACSQPKTEIEVGAKGEIEITE
                     EEKPFTIINLNNSSREDCPVSNQVVNIALTKVHFVQETQERIEQMNKLKEYLSQCSLS
                     VNKITVHTSFPSDFFYSSIDEAISELKE"
     gene            3614..5950
                     /gene="metE"
                     /locus_tag="PK513_07590"
     CDS             3614..5950
                     /gene="metE"
                     /locus_tag="PK513_07590"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOO82347.1"
                     /translation="MVLATNLGFPRIGPMRELKKAVEAYWKGKSSQDELLAIGKTLRA
                     QNWKLQQDAGLDHIPSNDFAFYDQVLSMTASLGLIPSRYQHSGGNVDLDTYFAMARGA
                     QTGSLDVTAMEMSKWYDTNYHYIVPEFEKGMSPKLSSTKIFDEYEEAKALGIETRPVL
                     IGPATYTFLGKPQYDGFKHEDFVQTLIPVYNEILAGLAERGCQWVQIDEPVFSLDMCP
                     IGQGTIKTAYANLKKPEGLKVLVATYFESLKDNAELAFGLPVDGIHIDLCRGSGRANT
                     TANDAGKDIEQALNLIGDEKILSLGVVDGRNVWKNSLANSLSKIEKAVSKIGSERVFV
                     APSCSLLHTPVDLDSETEMDDELKGWMAYATQKLDEIVTIAKGANEGRENVAAELAAS
                     NGAQESRKTSTRVHDKAVEERISNITEDMKERKSPFAKRQDVQHKALDLPLYPTTTIG
                     SFPQTPEIRKARSDFKKGVIDEAAYKKAMQDEIKMVVDFQHECNIDVLVHGEPERNDM
                     VEYFGEQLKGFAFSKYGWVQSYGSRCVKPPIIFGDVSRPVAMTVEWSKFAQSCTDKIM
                     KGMLTGPITILQWSFVRDDQPRNDTARQIALAIRDEVEDLEKAGIKMIQIDEAAFREG
                     LPLRRDDWKEYLDNAVENFRLTSCCVEDETQIHTHMCYSEFNDVIDSIGAMDADVISI
                     ECSRSQMELLEAFIEYKYPNEIGPGVYDIHSPRVPSVSEMVNLLEKAKLNLDERQIWV
                     NPDCGLKTRAWPETKEALKNMVAAAKKMREKSKGAKAA"
     gene            complement(6016..7314)
                     /locus_tag="PK513_07595"
     CDS             complement(6016..7314)
                     /locus_tag="PK513_07595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOO82348.1"
                     /translation="MKPASRIQATIDVLEKIAANTRVPMDSVIGDYMRVRRYIGSKDR
                     SEIAERIYNIARCHARLGWWLEKFSAEDTPRNRVLLWLMTAEGVDEKRLKDLFDASKY
                     APDELSPQETNYVKLIKEGKYIGGGIDHYDMPVEIKVECPPLYEDKLKAYFGDSFESE
                     MSAMLQPATLDLRVNTYLAPREKVINYLEADGVKTKETALSPWGLRCESKAYLAKTKA
                     FGKGWIEIQDEGSQAIATMCDVKPGMQVLDYCAGAGGKTLALGAAMQRKGRIVAMDND
                     ERRLKKGRDRYKKAQIADIIEIRCIEDEKNRKWLKRQKGKFDVVLTDVPCTGTGTWRR
                     NPDMRWRVFGPSLEELTEIQAEILGKVAPLVKQGGRLVYATCSLLPDENEVQIEKFLK
                     SHEDFEIVPVPENLGSPFMRLTPHRHGTDGFFTAILHRRV"
BASE COUNT         1987 a         1767 c         2034 g         1969 t
ORIGIN      
        1 cgaccacgga gatgttatgg ctcgtacgag tgcaggggca caaaaacagg atcgcgcacg
       61 taaaggagat gtgctggaac atagcgatca tggatgggaa gaagccaccg aacatgttcg
      121 ccgttggaaa gcacgcggtc tcaaagtcgg tttcactaat ggctgtttcg atattcttca
      181 ttatgggcac gtatcgtatc tcaataatgc gcgcgatcat tgtgaccgct tgattgtcgg
      241 cctcaataag gattcatcgg tgtgtgcttt aaaaggcccg cagcgccccg tccatgatga
      301 agaatcccgc gccggtgtgc ttgcggcgct cggctctgtc gatatggtgg tgctgtttgg
      361 cgcggataca gacggcgatg ataataccgc tatcaagctg ttagaggctc ttaaacctga
      421 catctatttc aaaggtggag attacacgga agatcagatc cctgaaagcc cgactgtacg
      481 aggctatggc ggcgaagtga acgttatgcc gatctatgaa ggccactcta ccaccggctc
      541 tatcgccaaa atcaataatg aggcagctta ggctcaggac ctataaacag gattccatag
      601 cgagttgatt tatgcccaac aaaggttaat aatcaatttt atattatgta tattattccc
      661 actcaattgt tccggggggt ttggaggtga tgtcgtaggt gacacggttg atgccggcaa
      721 cttcgttgat gatacgcgta gacacgcggg ataaaaattc atgctcgaac gggtagtaat
      781 cggcggtcat gccatcggta gaggtcaccg cgcgtagggc gcagacataa tcatatgtac
      841 gaccatcacc cataacacct acgctgcgca caggcagcaa caccgcaaaa gcctgccaga
      901 tttcatcata cagcccggcc tttttgattt cgtcgaggta gacggtgtcg gccttgcgca
      961 ggatttcgag tttgccacgg gtgatttctc cgggcatgcg gatggccagc cccggtcccg
     1021 ggaaggggtg gcggcggatg aaatcttccg gcatgccgag cgcacggcca agattgcgca
     1081 cttcgtcctt gaacagttca cgcatcggtt cgaccagttt gaggttcatg cgctctggca
     1141 ggccgccgac attgtgatgg gatttaatag ttacggatgg gccgccggtg aagctgacgc
     1201 tttcaatgac atcaggatag agggttccct gcgccaaaaa cgccgcatcg ccaacctctt
     1261 ttgcacattt atcaaacacg tcgataaaca gaccgccaat gattttgcgt ttggtttcag
     1321 gatcgcttac gccatcaagt ttgccgagga agagatcttc agcttcctcg tggatgagag
     1381 ggatattgta atgctcgcgg aacagcgcga cgacctgttc gctctcaccc gcgcgcatca
     1441 ggccggtatc gacatagatg caggtgagct ggtcgccgat tgcttcgtgt agtaaaatcg
     1501 cggtgacgga gctatccacg ccgccggaca aaccgcagat gactttttta tcgccgacct
     1561 gttcgcggat tttagcgatc gattcctcgc ggaaattttg catggtccag tccccggcgc
     1621 accccgcaac gttgtgggta aaatttttga acaaatcgcg gccatgaggc gtgtgcacga
     1681 cttcggggtg aaattggacg ccgtagaatt ttttgtcttt attggcgata acggcgaaag
     1741 gtgcaccttc ggatttggcc acaatatgga aaccttcagg gagatcagcc acgtggtcgc
     1801 cgtgggacat ccagacctgt tcacgcgcgc cagcatccca aacgccctgc gtaagatcgg
     1861 aatggtcggt cacctcgaca tgggcgcggc caaattcacc agcgccttcg ccgggggcaa
     1921 cgcgacctcc caattgtttg accatggttt gctggccata gcaaatccca aacacgggaa
     1981 caccaagggt aaagataatc tctggcgcgg cgggggcctc ttcgtcaagg acgctgcacg
     2041 ggccgccgga caggatgata cctttagggt tgaacgattt aatgcgatca tcatcagcgt
     2101 gattaaaagg ccatatttca caatagacgc cggactcgcg gacacgcctg gcaataagct
     2161 gggtaacttg cgagccaaaa tcgaggatca agatgtggtc gtgggtttta ttggtttcgc
     2221 ttgcgttttt ggggaccgtg acgttttccg cagacatgtc tttcctctcg gtaaaattta
     2281 tgaaattgta ttaagctaaa tttcaacaaa cgtaaaggtc tgtagagctt atgagcgcgc
     2341 caacaatatc tttcgaattt tttccgccta aaaccgagaa agccagtgaa gcgctctggg
     2401 aagcagtggc tgatctggcc gcgctggggc cgaaattcat gacagtgacc tatggcgcgg
     2461 gtgggtctac acgtgatggc acggtggata cgattgcgaa gatgaaagag gatacgggcc
     2521 ttcccatcgg ttcgcacctg acctttatca atacgccaaa ggatcaactg catgaattta
     2581 cgagcgcgct gtgggcagcg ggtattcgtc atatcgtggc gttgcgcggg gatatgccgg
     2641 atgatttaca atggccgctg gatgaggacg gtgagtattt ccagtatacg agtgattttg
     2701 tggaaggcct gaaaggctgg catgattttg agatttcggt cgggtgttat ccggaaaagc
     2761 acccggatgc gctggatatg agcgccgata ttgcggcgct gaaattaaag tgcgaagcgg
     2821 gcgcggaccg agccattacc cagtttttct ttgataatga tgtgtattat aattttcgcg
     2881 atgaatgcgc ggcggcaggc attgaaaccc cgattgtgcc agggttgttg ccgatccatg
     2941 atttcaagtc catgtgcaac ttcgccaaga agtgtcaggc gagtgtgcca gcaggccttc
     3001 atgaaaaatt tgcagggttg gaagacaagc cggaagaggc gcaaaaagtg gcggcggatt
     3061 tgttgattct gcaagcacag aatttagcgc aaaacggtgt ggaacacctc catttttata
     3121 cgctgaataa atcgatgatt acacaagagg ttgttcaatg cctcaacaat taaggcaggg
     3181 taaaaaaccg aagctgacta tattgatctc tatcttacta acgacagctt gttctcaacc
     3241 taagaccgag atagaggttg gcgctaaggg agaaattgaa attacagaag aggaaaaacc
     3301 ctttacgata attaatctca ataattcatc ccgagaagac tgccctgtaa gcaatcaagt
     3361 cgttaatatt gctctgacca aagtccattt tgtgcaagaa acacaggaga ggattgagca
     3421 aatgaataaa ttgaaagagt atttgtcaca atgttctctg tctgtaaaca agattaccgt
     3481 tcatacaagt ttcccctcag attttttcta ttcttccatt gatgaagcaa tttcagaatt
     3541 aaaagagtaa ttgcaatgcg gcctgtgtcc cgttaatctc acacggattt ttattttacc
     3601 aaggagttta aaaatggttc tggcaacaaa tttaggattt ccccgtatcg gtccgatgcg
     3661 cgagttgaaa aaagctgtag aagcgtattg gaaaggaaaa agctctcagg acgaattgct
     3721 ggccattggg aaaaccctgc gcgcgcaaaa ctggaagtta caacaggacg caggccttga
     3781 tcacatccca tcgaatgatt ttgcttttta cgatcaggtt ttaagtatga cagcatcgct
     3841 gggcttaatc ccttcgcgct atcaacacag cggcggcaat gttgacctgg atacatattt
     3901 tgcaatggcg cgcggcgcgc aaaccggcag tcttgatgtt acggccatgg aaatgtcgaa
     3961 atggtatgat acgaattacc attatatcgt gccggaattc gaaaaaggca tgagtccgaa
     4021 actctcctca accaaaattt ttgatgaata tgaagaagcc aaggctttag gcattgaaac
     4081 ccgtccggtc ttgattggcc ctgcgactta tacattctta ggtaagccgc aatatgacgg
     4141 gttcaagcat gaggacttcg tgcagacttt gataccggtt tataacgaaa ttctagccgg
     4201 gctggccgag cgtggttgtc aatgggtgca aatcgacgag cctgtgttca gccttgatat
     4261 gtgtccgatt ggtcagggca cgattaaaac cgcctatgcg aacctgaaaa aaccggaagg
     4321 gctgaaagtt ctggttgcga cctattttga gtccttgaaa gacaatgccg aactggcctt
     4381 tggtttgccg gttgatggga ttcatatcga tctgtgccgt ggctctggcc gggccaacac
     4441 cacggccaat gatgccggta aggatattga acaggctctt aacttgatcg gtgatgaaaa
     4501 gatcctgtct ttgggcgttg tagatggccg caacgtttgg aaaaacagtc tggccaattc
     4561 tttaagcaag attgaaaaag ccgtctctaa aatcggttcg gaacgtgtgt tcgtagcacc
     4621 gagttgctcc ttgctgcaca cgccagtaga cctagattct gaaaccgaaa tggatgatga
     4681 gttaaaaggt tggatggctt atgcgacgca aaagcttgac gaaattgtca caatcgccaa
     4741 aggcgctaat gaaggacgtg agaatgttgc ggccgaactg gcggcgagta acggcgcaca
     4801 agagtctcgt aagacttcaa cgcgcgttca tgataaagct gttgaggagc gaatttccaa
     4861 tattaccgag gatatgaagg aacgcaaatc tccgtttgcc aagcgtcagg atgtgcagca
     4921 taaggctttg gatctgccgc tttatccgac aacaacgatc ggctctttcc cgcagacacc
     4981 ggaaattcgt aaagcccgtt cagatttcaa aaaaggtgtt attgatgagg ccgcatataa
     5041 aaaggcgatg caggatgaga ttaaaatggt cgtcgatttc cagcatgagt gcaatattga
     5101 tgtgctggta cacggtgagc cggaacgtaa cgacatggtg gaatatttcg gcgaacagct
     5161 taaaggtttt gcgttttcga aatatggttg ggtgcagtcc tatggttcgc gttgcgtgaa
     5221 gccgccgatc atttttggtg atgtttcaag gcctgtcgcc atgactgtcg aatggtcgaa
     5281 atttgcgcag agctgcacgg ataagatcat gaagggcatg ttgaccgggc cgattacgat
     5341 tttgcaatgg tcgttcgttc gtgatgatca accccgtaat gatacagcgc gtcagattgc
     5401 gctggcgatc cgtgacgagg ttgaggatct ggaaaaagcc gggatcaaga tgatccagat
     5461 tgacgaagcc gcgtttcgtg aaggtttgcc tttgcgccgg gatgattgga aggaatatct
     5521 cgacaacgca gttgaaaatt tccgcctgac atcatgttgt gttgaagatg aaacgcaaat
     5581 ccatacgcat atgtgttatt ccgagttcaa tgatgtgatt gattctatcg gtgcgatgga
     5641 tgcggatgta atttcgattg agtgctctcg ttcacagatg gaacttctgg aggcgtttat
     5701 cgaatacaaa tatccaaacg agattggtcc gggcgtgtat gatattcatt ccccgcgtgt
     5761 gccgagcgtt tccgaaatgg ttaacctgct ggaaaaagcc aagcttaatc ttgatgagcg
     5821 ccaaatctgg gtaaatccgg attgcggtct aaaaacccgt gcttggccgg aaactaagga
     5881 agcgctgaag aacatggtgg ccgcagccaa aaaaatgcgc gaaaaatcga aaggcgcgaa
     5941 agctgcgtaa acaccacaaa ccactgggga cacttaccgg caagtgtccc tctacaattt
     6001 ccagaggcgt tttttttata cgcgacggtg taagatcgca gtgaaaaacc cgtctgtgcc
     6061 gtggcggtgg ggggttaagc gcatgaaggg gctgcccaaa ttttctggaa caggaacaat
     6121 ttcaaagtct tcatgacttt ttagaaattt ttcaatttgc acctcatttt cgtcgggaag
     6181 cagtgagcac gttgcataaa ccagtcgccc accttgttta acaaggggag cgactttgcc
     6241 caggatttcg gcttgaattt cagtgagttc ttccaggctt gggccaaaaa cgcgccagcg
     6301 catatccggg ttgcgccgcc atgtgcccgt gccggtgcaa ggcacgtccg ttaacacgac
     6361 atcaaatttg cctttttgcc gtttgagcca tttgcggttt ttttcatctt cgatgcagcg
     6421 gatctcgata atgtcggcga tttgcgcttt tttataacga tcacgtcctt ttttaagacg
     6481 tcgttcgtcg ttatccatag caacaatacg tcccttgcgc tgcatcgcag cgcccagcgc
     6541 aagagtcttg ccgcctgcac ccgcgcaata atcaagcacc tgcattcccg gtttaacatc
     6601 gcacatagtg gcgatggctt gtgagccttc gtcctgaatt tcaatccacc ctttgccaaa
     6661 ggctttggtt ttggcaagat aggccttgct ctcgcagcgc aacccccatg gagaaagcgc
     6721 ggtttctttt gttttaaccc cgtcagcttc gaggtaattg atgacttttt cacgtggcgc
     6781 caaataggtg tttacacgca gatccagcgt cgcgggctgg agcatcgcag acatttcact
     6841 ttcaaacgaa tcgccgaaat aggctttgag cttatcttca taaagtggtg gacactcgac
     6901 tttgatttca accggcatgt cgtaatgatc aatcccgccg ccgatatatt tgccctcttt
     6961 tattaatttc acataatttg tttcttgggg ggacaattcg tcaggagcat acttggatgc
     7021 gtcgaagaga tctttaaggc gtttttcatc gacgccctcg gccgtcatca accagagcaa
     7081 aacccggtta cgcggcgtat cttcggcgga gaatttctcc agccaccagc caaggcgggc
     7141 atggcagcgg gctatgttat agatgcgttc ggcaatttcg ctgcggtctt tcgaaccaat
     7201 ataacggcgt acacgcatat aatcgccaat tacagaatcc atcggaacgc gggtgttggc
     7261 agcgattttt tcaagcacat caatggtagc ctggattctg gatgcgggtt tcatggtcgc
     7321 tttatacacg cgaagatatt ggattcacag cagttttttt ctaattttgc accgggtttg
     7381 taacctgtga taaaaggaaa tcgaacagat gtccgaacac aacaaacaaa ttctattgca
     7441 agcaattttg tgatcgaggg tgcattagcc acaagacaaa gatgacacag gagaaaagca
     7501 atgaaatata caaaaccaca tagaccgcct ttgaaaattg gtgatgcgct ggtgtatggc
     7561 cgtcaggaca tggaccgttt aatgcgtgaa gttttggtta cgtaaggtat tcacatccct
     7621 aacccgatcc tgcatgatta aatcgattta aggcttttaa attctctcta agccccagcc
     7681 ccttacgctt taaaagtgga aagggttttc taactctcag ttcggtaatt gggcgcttcg
     7741 cgggtgatgg ttacgtc
//
LOCUS       DAOSWR010000097         7756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6043_length_7756_cov_5.065186, whole genome shotgun sequence.
ACCESSION   DAOSWR010000097 DAOSWR010000000
VERSION     DAOSWR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_7756_cov_5.065186"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PK513_07600"
     CDS             complement(<1..942)
                     /locus_tag="PK513_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82349.1"
                     /translation="MADITDSATSPDSEKKSVAPQESNDKTLTPKFKAADNGKTQPDT
                     NTSKNDAPTANAGNADDKIKPKFEEVTKKPAEEQKKKKKYEEPPLRELTDPEISSGYR
                     WIKDKDGKITGRAKHSDGQLDFGEAFSKLMHVIALLLSGDEMAPYMIDAIYPPEPKHK
                     DINDILADNQSGSPSKIADTQKEALKRKKEALKEVIEDKNHPDRQLAPEDFVSKYIGD
                     KEAIKAIEPQISKLFDLILSKESKGNLDVVNDYTGGTGLHPGDTTRGGLYVGILLDKD
                     GKQTFDPSRAVERKKFSESTINQVIAWQESERNCFCIS"
     gene            complement(1062..2918)
                     /locus_tag="PK513_07605"
     CDS             complement(1062..2918)
                     /locus_tag="PK513_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82350.1"
                     /translation="MSLLSYIEDKKPQILPENAPANTMAFASPQNTRDILQQTTTLNT
                     ISPDKFSANQLDRAQAALEKAPPEVVRELIADIADIETNVQSPQFEALFGTDGPAVQF
                     ILQNLALQGAGGSFVTAQSSTYAALDQDARKEREQRLRDTLIGGILFPPEIEEFFNQN
                     QNMFDAAAASILATDDVAEQAKIRAQLHEELFDKMRENPEFDKYTDEQLHDMIDAKFA
                     ESLAQQLRQQNPELSAAQARAQAEAMIQRMDEYAANDPAFAQTYNQYKNNSVAALYAQ
                     EQGITERIAKLPDEQRKIMEGVQSFRKDFVAVLALGEARLVYEEIENPGVYYVKDDDG
                     NNVYIDDLDPEYKDQAYIDIDRQLGQMFANQDLEGAAYAVKSVALYNQDETLQTMYVE
                     LTDGLYDQYASQYDLANQLTFLQNELSALENIIADKNTDEATSDAAFDRYLEIQDQID
                     ESLSSNQDENGNLTLDAPHPETMAQEQKVIDRARSYAAEQRSLGNTQTTSNDVTDAAR
                     PLSEKEQALAEKAVEQEGLAIEEPFNPNQYQSAADQYVPIEKPAATNTHPPAPAADTN
                     LKSDSEFDKQFAENKHMLTEQEDPSANNDLNIDMDAFSPTANTGSSSPGMMS"
     gene            complement(3068..4981)
                     /locus_tag="PK513_07610"
     CDS             complement(3068..4981)
                     /locus_tag="PK513_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82351.1"
                     /translation="MAENAEISANADSVKTLDFDGILATTTTFAISPTGLISEANWKL
                     IGNLGRQNLTGAFSGIATSHEGKETEEEREERKFREKLATTLLLSEIDRMIAEIEEQI
                     ANHEKQIAILLKDISGLEKENVELLAEIKKLKTTALPANDEAREALKESYKTYCDGLK
                     NLLDEDTQATLDRLKEIEGTFITVRADIKGIDGQRRYIVFKDEQSKNYIKHPKTGKAL
                     YINELDQPKMKLVDKLEGKSYSMQDDITMQEAQKDRRFGNDAHEGEVEWFYDGQNDLL
                     KALPEGDLRNDIIGFRDTLRYQEIQLFKLDQDREKMLEKIARLEKRVAENKRTINKNE
                     EKIAHHRQKIETLKEQLSALDERRNELISQTARIQEYTEEKREELTKLFTRAAEMQKA
                     GKTDAEILHELKEIDKSKTNALATIAMQMFKMRQNVENKQTEHSNVTETIENIEKIIK
                     DYPDRHEFLKKHFGINVFAVAGAWLNPITLSDEGVYRDNETGQLYTYNAKTNARTYIE
                     NPVIVAELYKRAYIDGELFTNETPTGDDPDNAFSQTWDAKLSTKQTTASLKSAEAKAE
                     FNLQAARSEAAALEQKTGISLSLSAARDEKAGELSLVYNRNETAQPNAPAETNILHKN
                     QILADPSTTNTPQ"
     gene            5214..6425
                     /gene="sucC"
                     /locus_tag="PK513_07615"
     CDS             5214..6425
                     /gene="sucC"
                     /locus_tag="PK513_07615"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOO82352.1"
                     /translation="MNIHEYQAKELLEKYGVAVPKGIAAMSVDEAVKAAEELQAGGTS
                     LFVVKAQIHAGGRGAGHFKGAADNKGGVRLCKSIEDVRAAAEAMMGNILVTKQTGPEG
                     KEVQRLYVTDGVDISKEYYCSLLLDRATAKVTFMVSTEGGMDIEEVAETHPEKIVKVA
                     IDPVGGITKADAEKLADALQLSGAAKDSAIPFFENLYKCYMELDASIVEINPMILAGD
                     EVMALDAKFNFDDNALFRHPDIVALRDESEEDENEKIAADNELSYVRLDGNIGCMVNG
                     AGLAMATMDIIKLKGGEPANFLDVGGGATAERVTVAFKIILSDPNVKGILVNIFGGIM
                     KCDVIAEGVIAAAKEVNLSVPLVVRLEGTNVDKGKALMASSGLPIISADDLEDAASKV
                     VAATEKAQAAA"
     gene            6455..6646
                     /locus_tag="PK513_07620"
     CDS             6455..6646
                     /locus_tag="PK513_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82353.1"
                     /translation="MADDGTGKVRYTGLVSGNKLAEMFQRGKSTAASQVGDELVGEAP
                     GSVAFDQVGIVYDDPASTL"
     gene            6913..>7756
                     /gene="sucD"
                     /locus_tag="PK513_07625"
     CDS             6913..>7756
                     /gene="sucD"
                     /locus_tag="PK513_07625"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOO82354.1"
                     /translation="MSVLVDKNTKVICQGFTGAQGTFHSEQAIAYGTNMVGGVTPKKG
                     GQEHLGLPVFNTVKEAKDATGCNATVIYVPPPFAAAAIVEAIEAEMELAICITEGIPV
                     LDMVKVKAALKGSKTRLIGPNCPGIITPDECKIGIMPGHIHTRGKVGIVSRSGTLTYE
                     AVAQTGAVGLGQSTCIGIGGDPVNGTNFIDCIDMFMNDPETEAILMIGEIGGTAEIEA
                     AEYYQGLKTKKPIAGFIAGATAPPGKRMGHAGAIISGADDTAPAKMKKMRECGFVVSE
                     SPAGL"
BASE COUNT         1581 a         1526 c         2136 g         2513 t
ORIGIN      
        1 ggatatgcaa aagcagttcc tttcgctttc ctgccaggca attacctggt tgatggttga
       61 ttctgagaat tttttgcgtt ctactgcccg gcttggatca aatgtttgtt ttccgtcttt
      121 gtcgaggagg atgccaacat acaagccgcc tcgcgttgtg tctcccggat gcaacccggt
      181 gccgcctgtg taatcgttaa cgacatcgag attgcctttt gattctttgg acaggatcaa
      241 atcaaagagt ttgctgattt gcggttcgat cgctttgatg gcttctttgt cgccgatata
      301 tttgctgaca aaatcttctg gtgcaagctg gcggtccggg tggttcttgt cttcgatgac
      361 ttctttgaga gcttcttttt tacgtttcaa ggcttctttt tgcgtgtcgg cgattttgga
      421 aggagaaccg gactggttgt cggcgagaat gtcgttgatg tctttgtgtt ttggctctgg
      481 tggatagatt gcgtctatca tgtagggagc catttcgtca ccagataaaa gcagggcaat
      541 gacatgcatg agttttgaaa aggcttcacc aaaatctaat tgaccgtcgg agtgtttggc
      601 gcgtccggtg atttttccat ctttatcttt gatccaccgg tagccgctag atatttccgg
      661 gtcggtgagt tcgcgcagcg gtggctcttc gtattttttc tttttctttt gttcttctgc
      721 gggctttttt gtgacttctt caaatttcgg tttgatcttg tcatctgcgt ttccagcatt
      781 tgcggtcggc gcatcgttct tggaagtgtt tgtatccggc tgcgttttgc cgttatctgc
      841 ggctttgaat ttgggcgtaa gtgttttatc gttgctttct tgtggcgcca cgctcttttt
      901 ttcactgtcg ggagatgtcg ctgaatctgt tatatcagcc atggtcacac cctctttttt
      961 actgccgttt tattttttgt tctttttcgt ttcccctatt atgggcaaaa agggttaatt
     1021 tttggttaat attgtatggt aacggcaggg tgacggtcgt tttagctcat catgccgggg
     1081 ctggagcttc cggtatttgc tgttggcgag aatgcatcca tgtcgatatt cagatcatta
     1141 ttagccgagg gatcttcctg ttcggttagc atgtgcttgt tttcagcaaa ttgtttgtcg
     1201 aattccgagt ctgatttgag gtttgtgtct gctgccggag cgggggggtg cgtatttgtt
     1261 gccgccggtt tttctatcgg tacatattgg tctgctgctg attggtactg atttgggttg
     1321 aaaggctctt cgattgcgag accttcttgt tctacagcct tttctgcaag ggcttgttct
     1381 ttttcgctta atggtctcgc agcgtcggta acgtcgtttg aggttgtctg tgtgttgcct
     1441 agagagcgtt gttctgcggc ataagagcgg gcgcggtcta taactttttg ttcttgtgcc
     1501 atcgtttccg ggtgaggagc atcaagagtc aggttgccgt tttcgtcctg gttgcttgac
     1561 agagattcat caatttgatc ctgaatttca aggtaacggt caaaggcagc gtctgatgtt
     1621 gcttcatcag tgtttttgtc agctatgatg ttttctaaag ctgataactc attttgtaaa
     1681 aatgtaagct ggtttgccag atcgtattgg ctggcatatt gatcatacag gccatcggtc
     1741 agttccacgt acatggtttg tagggtttca tcctgattgt ataatgcaac agattttacc
     1801 gcatatgcgg cgccttccag gtcttggtta gcaaacatct gacctaattg acggtctatg
     1861 tctatgtatg cctggtcttt gtattcgggg tcgagatcat caatgtaaac attattaccg
     1921 tcatcatctt tgacgtaata tacgccggga ttttctattt cttcataaac gagacgggct
     1981 tcacccaagg ccaggacagc tacgaagtcc tttctgaagc tttgaacgcc ttccatgatt
     2041 ttgcgctgtt cgtcgggtag ttttgcaatt cgctcggtta tgccttgttc ttgagcgtaa
     2101 agagcggcaa cagagttgtt tttatattga ttataagttt gtgcaaaagc cgggtcgttt
     2161 gcggcatatt cgtccattct ttggatcatt gcttcagctt gtgcgcgcgc ttgtgcggca
     2221 gataattcgg gattttgttg tcgtaattgt tgtgccaaag actccgcgaa ttttgcatca
     2281 atcatatcat ggagctgttc atctgtgtat ttatcgaact cagggttttc acgcatttta
     2341 tcaaagagtt cctcatgaag ctgcgctctg attttggctt gttccgcaac atcatcggtt
     2401 gcaagaatgc ttgctgctgc ggcatcgaac atgttttgat tttgattgaa gaattcttcg
     2461 atttcgggtg gaaacaatat gccgcctatt aatgtgtctc tcaggcgttg ttcgcgttct
     2521 ttccgggcat cttggtctaa tgcggcgtag gtggatgatt gtgcggttac aaaagagccg
     2581 cctgcgcctt gcagcgctaa attctggagt atgaattgca cagccggacc atcggtgccg
     2641 aagagggctt cgaattgcgg gctttgtaca ttcgtttcta tatcggctat atctgcgatg
     2701 agttcgcgta cgacttccgg cggcgctttt tccaacgctg cttgtgcacg atctaactga
     2761 ttggcagaga atttgtcagg tgaaattgta tttagtgtgg ttgtttgctg gaggatgtcg
     2821 cgggtgtttt gcggggatgc aaaagccatc gtgtttgcag gcgcattctc cggcaaaatt
     2881 tgtggttttt tatcttctat gtaacttagt agcgacactt agtcccacac ccttttttat
     2941 ttttctttgg atttccaaac cttgtttgaa aaacatttgc acgcgtttat cataatactt
     3001 aaaagtttaa gttttattaa cgctttgaaa ataaatttta gaggttccgg ggcgcaaatg
     3061 ggtgatttta ttgtggtgtg tttgttgtcg atgggtctgc aagtatttga tttttatgta
     3121 atatattggt ttctgcgggc gcgttaggtt gtgcggtttc gtttcgattg taaacaagag
     3181 ataattcacc ggctttttcg tcacgtgctg cggacaggga tagagaaatg ccggtttttt
     3241 gctccagggc tgcggcttcg ctacgcgcgg cttgcaggtt aaattcagct ttagcttctg
     3301 cgcttttcaa gcttgctgtg gtttgttttg tggagagctt tgcgtcccat gtttgcgaga
     3361 atgcattgtc tggatcgtcg cctgttggtg tttcgttcgt gaataattcg ccatcgatat
     3421 aggcgcgttt atacagttcg gcaacgatta cagggttttc gatataggtg cgggcgtttg
     3481 tttttgcgtt gtaggtataa agctggcctg tttcattgtc gcgatagacg ccttcatcgc
     3541 tcaaggtaat cgggtttagc cacgctccgg ccactgcaaa gacattgata ccaaaatgtt
     3601 ttttcaggaa ttcgtggcga tcagggtagt ctttgattat tttttcaata ttttcaattg
     3661 tttctgtgac gttgctatgc tctgtctgtt tgttttcgac attctggcgc attttgaaca
     3721 tttgcatggc gatggtggcc agagcgttgg ttttgctttt gtctatttct tttaattcgt
     3781 gaaggatttc cgcatctgtt ttgccggctt tttgcatttc ggcggcgcgg gtgaagagct
     3841 ttgttaattc ttcgcgtttt tcttcggtgt attcttgtat gcgggctgtt tgtgagatta
     3901 gctcattgcg gcgttcatcc aacgcgctta gttgttcttt aagtgtttca attttttgtc
     3961 tgtgatgtgc aattttttct tcatttttgt ttattgtgcg cttgttttcg gccactcttt
     4021 tttccagacg cgcaattttt tcgagcattt tctctcggtc ttggtcgagt ttgaaaagct
     4081 ggatttcctg atagcgtaaa gtatcgcgga agccgatgat atcattgcgc agatcacctt
     4141 caggaagagc ctttaacaga tcattttgtc cgtcataaaa ccattcgact tcgccttcat
     4201 gggcgtcatt gccaaaacgc cggtcttttt gcgcttcttg catggtaatg tcgtcctgca
     4261 tcgagtagct tttgccttca agtttatcga caagcttcat tttaggctga tctagttcgt
     4321 ttatataaag cgctttacct gttttggggt gtttgatgta atttttgctc tgttcgtctt
     4381 tgaaaacgat gtagcggcgc tggccgtcga tccctttaat atcggcgcgt acggtaataa
     4441 aggttccttc gatttcttta agtctatcga gcgttgcttg cgtgtcttca tcaagaaggt
     4501 ttttaagtcc gtcgcaatag gttttgtacg attctttgag tgcttcacgg gcttcatcat
     4561 tggccggtaa agcggttgtc tttagttttt taatttcggc cagcagttct acattttctt
     4621 tttctaggcc tgaaatgtct ttgagtagta ttgcgatttg cttttcgtgg tttgcgattt
     4681 gttcttcgat ttctgcgatc atgcggtcga tttcgctcag caggagggtg gttgcgagtt
     4741 tttcgcggaa ctttctttct tcgcgctctt cttcggtttc ttttccttca tggctggtgg
     4801 cgattcccga gaatgcgccg gttaaattct gcctgccgag attgccaatg agtttccaat
     4861 tcgcctcgga gattaaacct gttggagaga ttgcaaaggt tgttgttgtg gctaaaatgc
     4921 cgtcgaaatc cagtgttttt actgaatctg catttgcaga gatttcggcg ttttctgcca
     4981 tttatcaacc cttgttctta ccgtttgtcc ggatgcgttt atgccgcacc atctattttc
     5041 atatatcgca tcgtaatgga taaaatttaa gatatggtta atggcctgtt aagaaattgg
     5101 gaaatattgg cgcaaaaaca tgtatgcagg gctgtttacg ggttgcaatg cgcgcagagt
     5161 gcattagtct tgcgggtgag attctttacc caaactactg gagcaactta tccatgaata
     5221 tccatgaata tcaggccaaa gagctgttag agaaatatgg cgttgcggtg cctaaaggca
     5281 ttgctgcgat gagcgttgat gaagctgtta aagcggcgga agagcttcag gccggcggga
     5341 cgagcctgtt tgttgtgaag gcgcagatcc atgcgggtgg gcgcggggct ggccacttta
     5401 agggcgctgc ggataacaag ggcggcgttc gtttgtgtaa gagcattgag gatgtgcgcg
     5461 cggcggctga agcgatgatg ggcaacatat tggtgaccaa gcaaaccggc ccggaaggga
     5521 aagaggttca gcgtctttat gtgacggacg gggtggatat ttccaaggaa tattattgtt
     5581 cgttattgct ggatcgggct acagccaagg tgacctttat ggtttccacc gagggcggaa
     5641 tggatattga ggaagttgcc gaaacgcatc cggaaaagat cgtcaaggtg gcgattgatc
     5701 cggtcggcgg cattaccaag gccgatgctg aaaagctggc cgatgctctg cagctgagcg
     5761 gtgcggcgaa agacagcgcc attccgttct ttgaaaatct ttacaaatgc tacatggagc
     5821 tggatgcctc aatcgttgag atcaatccga tgattctggc tggggatgaa gtgatggcgt
     5881 tggatgcgaa gtttaacttt gacgataacg cgttgttccg tcatccagat atcgttgcgc
     5941 ttcgtgacga gagcgaagag gacgagaacg agaaaatcgc ggcggataat gagctgtctt
     6001 atgtgcgttt ggacggcaat attggctgta tggtcaatgg ggcgggtctt gcgatggcga
     6061 cgatggatat aatcaagctg aaaggcggcg agccggcgaa ctttcttgat gtcggtggcg
     6121 gcgccacggc tgagcgcgtg acggtcgcgt ttaaaatcat tttatccgat ccgaacgtaa
     6181 agggaattct tgtgaatatc tttggtggaa tcatgaaatg tgatgtgatc gctgagggcg
     6241 tgattgcggc ggccaaggaa gtgaatttga gtgtgccgct ggtcgtgcgt ttggagggga
     6301 caaatgttga caaaggcaaa gcgcttatgg caagttccgg tttgccgatt atttctgcgg
     6361 atgatctgga agatgcggcg agcaaggtgg tcgcggcgac tgaaaaagcc caagcggcgg
     6421 cttagttttt gttttaactt aaggagaaaa atgtatggct gatgatggaa cggggaaggt
     6481 gcggtatacg gggcttgttt ctggaaacaa attagcggag atgtttcagc gcggaaaatc
     6541 tactgcggct tctcaagtgg gcgatgagct tgtgggtgaa gcgccaggga gtgttgcttt
     6601 tgatcaggtt ggtattgttt atgatgatcc tgcttcgact ctctaaggtc ttgcgttatc
     6661 gctaatggct ttgaggtgat ctgggatttt ctttgaaacg ccagattgtt ttggttgccg
     6721 ggtttattca cagtaattgg tgctctgaaa gggcaaggtt cttaatttga aagagcataa
     6781 gagtttgcaa ttcccgccga tgtgcgggaa tttttttgtg cccaatgttg aaaacctgat
     6841 gctgggatgt tgattttact tcagggcgct atataaaaaa gagagcgcaa cgctagaaaa
     6901 ttgaggaatc ttatgtccgt actcgttgat aaaaatacca aagtgatttg ccaaggtttt
     6961 accggggcac aaggcacgtt tcatagtgag caagcgattg cttacggtac caacatggtt
     7021 ggcggggtaa caccgaaaaa aggcgggcag gagcatttgg gcttgccggt tttcaatacg
     7081 gtgaaagaag ccaaggacgc tacgggctgc aatgcgacgg tgatctatgt gccgccgcca
     7141 tttgcggctg cggcgattgt tgaggctatt gaggccgaga tggagctggc gatttgcatc
     7201 accgaaggca ttccggtgct ggatatggtg aaggtgaagg cggcgctgaa aggctccaaa
     7261 actcgtttga tcggcccgaa ctgtccgggg attattacgc cggatgagtg caagatcggg
     7321 attatgcccg ggcatattca tacgcgcggc aaggtcggga ttgtctcgcg ttccgggacg
     7381 ctgacgtatg aagctgtggc gcagaccggc gcggtcggtc ttgggcaatc gacctgtatc
     7441 ggcattggcg gggatccggt gaacgggacg aactttatcg attgtatcga tatgtttatg
     7501 aacgatccgg aaaccgaagc tattttgatg atcggcgaga ttggcgggac agccgagatt
     7561 gaagcggcgg aatattatca gggcttaaaa accaagaagc cgattgccgg gtttatcgcc
     7621 ggggcgactg cgcctccggg caaacgcatg ggccatgcgg gtgcgattat ttccggcgca
     7681 gatgatacgg cgccggcgaa gatgaagaaa atgcgcgagt gcggttttgt ggtttccgaa
     7741 agccctgccg ggttgg
//
LOCUS       DAOSWR010000098         7700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6121_length_7700_cov_5.689993, whole genome shotgun sequence.
ACCESSION   DAOSWR010000098 DAOSWR010000000
VERSION     DAOSWR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6121_length_7700_cov_5.689993"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK513_07630"
     CDS             complement(<1..253)
                     /locus_tag="PK513_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82355.1"
                     /translation="MEFVNKDADMILVNTTPEELKKLAAIMRACHEFDFVLCEMDSEH
                     LLALAERFEKTEENSWVFHDMELFYIRNILNLAIRGMGIE"
     gene            complement(308..658)
                     /locus_tag="PK513_07635"
     CDS             complement(308..658)
                     /locus_tag="PK513_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82356.1"
                     /translation="MEFVNKDADMVLVNTTPEELKKLAAIMRACHSYDPVLCEMDSDD
                     LLDLADRLDKAEQDSWVFHDLEILYLYNILIFARSLGDECRSSSHYFNNVTEQDIVYM
                     WAQLKKIDELRHLA"
     gene            748..2154
                     /gene="thrC"
                     /locus_tag="PK513_07640"
     CDS             748..2154
                     /gene="thrC"
                     /locus_tag="PK513_07640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO82357.1"
                     /translation="MNYISTRGGGEPRDFEQALLSGLAPDGGLYVPESWPRLDLDALK
                     GKPYIDVAIEVMYPFVEGSLDRDEFATIVRETYGPEFFRHKDVVPLHPLKDNHYILEQ
                     FHGPTIAFKDVALQLLGRLFDHVLKKRGEHITIVGATSGDTGSAAIEGCKVSDQLDIF
                     ILHPKGRVSDVQRRQMTTVEGENVHNIALEGSFDDCQNMVKAMFNDAEFRKEMNLAAV
                     NSINWARVMAQIVYYVTSALELGAPDKQVTYVVPTGNYGNVFAAYCARKMGLPIKLVV
                     ATNENDILTRFFESGQMKAAGCVATITPSMDIEISSNFERYLFDLLDRDEDALAAMMR
                     RFKDTGHFEVSPEQLARAREDFAAGRTDQSETLATIKQVYEDTDYILDPHTAVAMKVA
                     LDVAPEVDGPVVTLATAHPAKFPDAVEKAIGLRPALPEHLADLFDRAERLEELPNDIK
                     VVQDFVREHSHISKQNAA"
     gene            2160..3422
                     /locus_tag="PK513_07645"
     CDS             2160..3422
                     /locus_tag="PK513_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO82358.1"
                     /translation="MSNIQTTTLPNGLRVITDHVPSMHSVALGIWAGVGTRHENLAQN
                     GVAHMVEHMLFKGTEMRDALQIAEELENVGGSMNAYTGREVTSYHVHLLAEDMRLGLD
                     VMCDMYLNSTLPEDEIEREREVILQEIGMCNDTPDDLVFDVFSETAYPDQALGAPILG
                     RAHNIEAMSQDHLRDYIDRFYTPANTVVSAAGAVDHDAFVAQAEDAFADKAASRACTY
                     EAAAYKGGETRLNKDLEQSHFILGFQGLGRHEDDYYAAQALSTVLGSGMSSHLFQEVR
                     EKRGLVYSIFSYHSGYQDNGTFGIYAGTGPDKLDEIIPIVCDEVLKVGSDVMEEELAR
                     AKSQLKAGLLMGRESMMTRADQQAKCLLFRNKVFDLESLTGAIEKIDRAAIARVAEKI
                     FSTKPTLAALGPLEKLEAFEQVRKRLAA"
     gene            3426..4031
                     /locus_tag="PK513_07650"
     CDS             3426..4031
                     /locus_tag="PK513_07650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO82359.1"
                     /translation="MLLGFKKQDSEFPDISLQCARVLMRPPTLTDWPEWVKVRAVNQD
                     HIQPFEPLWPSEPFTQDLFKRRLARQIREWELGHANAFLIFKRDNGQLIGGMNINNIC
                     RGAAQYASLGYWIDWNHEGRGYMAESLALTLRYCFETLGLHRVNASCLAHNTRSRNVL
                     VKAGFQEEGMAEKYLQINGEWQDHILFGLPIEHWKEVEGGD"
     gene            complement(4028..4609)
                     /locus_tag="PK513_07655"
     CDS             complement(4028..4609)
                     /locus_tag="PK513_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOO82360.1"
                     /translation="MTETKTGYLTGKLLLAMPTIGDPRFHRAVIYLCAHDENGAMGLV
                     INAPVPALKFDDLLKQLGIESDIEIKAKDLNLPVLNGGPVETARGFLLHSTEFKQDDT
                     VQVDEDFSITGTTDALKAVAKGEGPDQALFILGYAGWTPGQLEAELQQNAWLVSQPDP
                     ALIFTDKAEEKWEHAVHKLGFDPALLSGEMGRA"
     gene            4692..5153
                     /locus_tag="PK513_07660"
     CDS             4692..5153
                     /locus_tag="PK513_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOO82361.1"
                     /translation="MSKFFIFLIVFAFAMPAWASDWVDGEHVRARLAAVPDAGNKMAV
                     LEVELEDGWHSYGQDPGDAGLPPRFEWEGSSNLERAQISWPETFEKREMDMFTVNAYE
                     GAIQFPLTIIPVDVQGDVALKLGLKLMVCKDICIPSTLDLALDLKPAWPAP"
     gene            complement(5150..6265)
                     /locus_tag="PK513_07665"
     CDS             complement(5150..6265)
                     /locus_tag="PK513_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO82362.1"
                     /translation="MAAKKLLLVINHIDWFWSHRLPLAKGAQVKGWDVSVAVTGADSD
                     PDLAPQHFTGHELPASGPAKIIWAIYKIIKREHPAIIHAITLKYAFFAGLAARLYKDV
                     RVVHTLAGLGYLFSGEGAKPQILRIAIGPFLKLALKQKRTHLIFQNPDDMALMIRRRF
                     AVPERCTLIRGSGVDLTQYPLMPLPEDKRPIVLMPTRLIHEKGVAVFIKAANILKKRG
                     INARFKIAGGLSATTPSAISKEEMETMLAESEVEWLGKVADMPALYQSASLICYPSWY
                     GEGVPKVLLEAAATGRAIVTTDHPGCREAVEHEVNGLLVPVKDPLATADAMRVLLEDR
                     PCLEAMGRASHDLAARNFDVESVVAHTLKIYEEISKA"
     gene            complement(6266..7261)
                     /locus_tag="PK513_07670"
     CDS             complement(6266..7261)
                     /locus_tag="PK513_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017007807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO82363.1"
                     /translation="MKIAITGGAGFIGTQLAKLLKSDGHELVLIDLKKSESFPDDSII
                     ADIRDLDTLTKALEGADAIYHLAAEHRDDVTPLSLYTDVNVTGGQNVIEAAKANGIKK
                     IIFTSSVAVYPLVPADLKKGSSEEHPPAPFNPYGQSKCDSEKTFEYWASQDPARSLTI
                     IRLVATFGPGNRGNIFTLINQIASGKFIMIGDGKNCKSIAYVGNVAAFLHHALGFKAG
                     AHLYNYADKPDINMRDFVASIRKALGYRGLGPQMSYALGLLGGSVFDVAAKVTGKKFP
                     ISIIRIKKFCADTIVNADKLKEETGFKAPYSLHDGLEEMIECEFQDKKQDHKRAA"
     gene            complement(7277..>7700)
                     /locus_tag="PK513_07675"
     CDS             complement(7277..>7700)
                     /locus_tag="PK513_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOO82364.1"
                     /translation="YLPHDILTKVDRASMAYGLEARAPLLDTRIFDYVWSLPENIKIR
                     GGQGKWLLRQVLKRHVPEKLFQRPKQGFNMPVGDWLRGDLRDWAESLLDEKHLREEGL
                     LDVQKTRKLWQSHLGAQGAHGEALWCILMFQAWHKRWM"
BASE COUNT         1822 a         1769 c         2118 g         1991 t
ORIGIN      
        1 gttcaatacc catcccgcga atggccaaat ttaaaatatt acgtatgtaa aacagctcca
       61 tatcgtgaaa cacccagctg ttttcttcgg tcttttcaaa acgttcggcc aaggcgagta
      121 aatgctccga atccatctcg cataatacaa aatcaaattc gtggcacgcc cgcataatcg
      181 ccgcgagctt tttcagttct tcaggcgttg tgttgaccaa aatcatatcg gcgtctttgt
      241 taacaaattc catggattct gatctttgta tgttcttatg cgataaacta tcagacaaac
      301 tgcaaagcta tgcaagatgc ctgagttcat caattttttt taattgtgcc cacatgtaca
      361 ctatatcttg ctctgtaaca ttattaaaat aatgggatga agaacgacac tcgtctccaa
      421 ggctgcgggc aaatataaga atattataga gatagagtat ctctaaatca tgaaaaaccc
      481 agctatcctg ttctgcctta tcaagacgat ctgcgagatc taacagatcg tcactgtcca
      541 tttcacacaa aacagggtca taggaatgac acgcccgcat aatcgccgcg agctttttca
      601 gttcttcggg cgttgtgttt accaaaacca tatcagcgtc tttgttaacg aattccatgg
      661 atgccagcct ttatttattc tctgcagtat agcgtaatcg catgtgattg acaccctata
      721 tgaaacccct tacaaactgc ctttatgatt aattatattt ccacgcgcgg gggcggcgag
      781 ccgagagact ttgaacaagc tttactgagt gggctggcgc ctgatggcgg cttgtatgtg
      841 ccggaaagct ggccgcgcct tgatctggat gcacttaaag gtaaaccgta tatcgatgtt
      901 gccatagagg tgatgtatcc gtttgtggaa gggtctttgg accgcgatga atttgcgacg
      961 attgtgcgtg agacctatgg gccggaattt ttccgtcaca aagatgtcgt gccactacac
     1021 cctcttaaag acaatcacta tattttggag cagttccacg ggccgacgat tgcgtttaag
     1081 gatgtggcgt tgcaattgtt agggcgattg tttgaccatg ttttgaaaaa gcgcggtgag
     1141 catatcacga ttgtcggcgc gacctcgggc gatacgggca gcgcggcgat tgaagggtgc
     1201 aaggtttccg atcagctgga tatttttatc ctgcacccga aggggcgcgt ttcagatgtg
     1261 cagcgtaggc agatgaccac cgtagagggc gagaatgtgc ataacatcgc gctggaggga
     1321 tcgtttgatg attgccagaa catggttaag gcgatgttta atgatgcgga gtttcgtaaa
     1381 gagatgaatc tggctgccgt gaactctatc aactgggcgc gggtgatggc gcagattgtt
     1441 tattatgtga ccagcgcttt ggaactgggc gcgccggaca aacaggtgac ttatgttgtg
     1501 ccgacgggca attacggcaa tgtgtttgcg gcgtattgcg cgcgtaagat gggccttcca
     1561 atcaagctgg tcgtggcgac gaatgagaat gacatcctga cgcgattttt tgaaagcgga
     1621 cagatgaagg ctgcggggtg cgtggcgacg attacgccgt cgatggatat tgagatttcg
     1681 agcaattttg agcgttatct ctttgatctt ctggataggg atgaggatgc gttggccgcg
     1741 atgatgcgcc gctttaagga caccgggcat tttgaagtat cgcctgaaca actggcgcgg
     1801 gcgcgtgaag atttcgcggc cggacgcacg gatcaaagcg agacgctggc gacaattaaa
     1861 caggtctatg aggacactga ttatattctc gatccgcata cggcggtggc gatgaaggtg
     1921 gcgctggacg ttgcgcctga agttgacggg ccggtggtga cgctggccac ggcgcatccg
     1981 gcgaagtttc ctgatgcggt ggaaaaagca attggtctgc gccctgccct gcctgagcat
     2041 ctggcggatt tgtttgaccg cgccgagcgg cttgaagaat tgccaaatga cattaaagtc
     2101 gtgcaggatt ttgtgcgcga gcattcacat atatccaaac aaaatgcggc ttaacctcta
     2161 tgagtaatat tcaaacgaca actcttccca acggcctgcg ggttattacc gaccatgtgc
     2221 cgagtatgca ttcggtggcg ctcggcatct gggctggtgt gggtacgcgg catgaaaacc
     2281 tcgcgcaaaa cggcgtggcg catatggtgg aacatatgct gtttaaaggc acggagatgc
     2341 gcgatgcgct ccagattgcc gaagagctgg agaatgttgg cggatctatg aatgcgtata
     2401 cagggcgtga agtgaccagc tatcacgtgc atttactggc cgaagacatg cggttggggc
     2461 tggatgtgat gtgcgatatg tatctgaact cgacgctgcc tgaagatgag atcgagcgcg
     2521 agcgcgaagt gattttgcag gaaatcggga tgtgtaatga tacacccgat gatctggtgt
     2581 ttgatgtttt ttctgaaacg gcttatccgg atcaggcttt gggcgcgccg attttaggcc
     2641 gggcgcataa tattgaagcc atgagtcagg accatctgcg cgattatatc gatcggtttt
     2701 acacgcccgc caacacggtg gtttcagcgg cgggcgcggt tgatcatgat gcatttgtgg
     2761 cgcaggcgga ggatgccttt gccgataaag cggcttcgag agcctgcact tatgaagcag
     2821 cggcctataa aggcggagaa acgcggctga ataaggatct tgagcagagc catttcattc
     2881 ttggttttca ggggcttggc cgtcatgaag acgattatta tgcagcacaa gcgctttcga
     2941 cggtgctggg gtcgggcatg tcttcgcacc tgtttcagga agtgcgggaa aaacgcgggc
     3001 tggtctattc gattttttcc tatcacagcg gttatcagga taatggcaca ttcgggattt
     3061 atgccggaac cgggccggac aaactggatg agattattcc aattgtttgc gatgaggtgc
     3121 tgaaggttgg cagcgatgtg atggaggaag aactggcccg agcgaaatca caacttaaag
     3181 cgggactgtt gatgggaagg gaatcgatga tgacccgcgc cgatcaacaa gctaaatgtc
     3241 tattgttccg caataaggtt tttgatttgg aaagtttgac gggagctatt gagaaaattg
     3301 acagggcggc gattgcgcgc gttgccgaaa aaatattttc tacaaaacca acgctagcag
     3361 cgctcggccc gcttgaaaag cttgaagcat ttgagcaagt tcggaaacga ttggccgcct
     3421 aaaagatgtt gctcggtttt aaaaaacaag attctgagtt tcccgatatt tcactgcaat
     3481 gcgcgcgcgt tttgatgcgc ccgccgactt tgaccgactg gccggaatgg gtaaaagtgc
     3541 gcgcggtcaa tcaagatcat attcagcctt ttgagccgct atggccgagc gaaccgttta
     3601 cccaagacct ttttaaacgc cgtctggcgc gtcagattcg cgaatgggaa ttggggcatg
     3661 caaatgcatt tttgattttt aagcgcgaca atggccaatt gatcggcggg atgaatatca
     3721 acaatatctg ccgcggcgcg gcgcagtatg cttctttggg ttactggatt gattggaatc
     3781 atgaaggccg gggctatatg gcagaaagcc ttgcacttac tttgcgttat tgttttgaaa
     3841 cgctgggact gcaccgggtg aatgcgtcgt gtttggcgca caatacgcgc agccgcaatg
     3901 tgctggtaaa ggctgggttt caggaagagg gcatggcgga gaaatatcta caaatcaacg
     3961 gggaatggca ggaccatatt ttattcggtc tgccgattga gcattggaaa gaggttgagg
     4021 gtggcgatta agcgcggccc atttcacccg agagcaacgc aggatcaaaa ccaagcttgt
     4081 gcacagcgtg ttcccatttt tcttcggctt tatctgtaaa gatcagcgcc gggtctggct
     4141 gtgaaacaag ccaggcattt tgctgtaatt ccgcttcaag ctggccgggc gtccatccgg
     4201 catagcctaa aataaagagc gcctggtccg ggccttcgcc tttggccacg gccttaagcg
     4261 catcggtggt gccggtgatg ctgaaatctt catcgacctg cacggtgtca tcttgtttga
     4321 attcggttga atggagcaaa aatccacgtg ccgtttcgac aggtccgccg tttaagacag
     4381 gtaggttcaa gtctttggcc ttgatttcaa tatccgattc aatgccgagt tgtttgagca
     4441 gatcgtcaaa ttttaatgca ggtacaggag cattgatcac cagccccata gcgccgtttt
     4501 catcatgggc gcatagataa atcacagcgc ggtgaaaacg tggatcaccg atcgtcggca
     4561 tggccaagag cagctttccg gttagataac ccgtctttgt ttctgtcatc gtcacctctt
     4621 cgtttgcctc gcatgtgcaa aaagattatc cttaaaagag gcggttcaca ttaacaggaa
     4681 tgagcggtta gatgtcaaag ttttttattt ttctaattgt ctttgctttt gcaatgcctg
     4741 catgggcttc ggattgggtg gatggcgagc atgtgcgcgc acgtctggcc gctgtgcctg
     4801 atgctggaaa taaaatggct gttttggaag ttgaattgga ggacggttgg catagctatg
     4861 ggcaagatcc aggtgatgcg ggtttgccgc cgcgctttga gtgggaaggc tcaagcaatc
     4921 ttgagcgcgc ccagatcagt tggccggaga cgtttgaaaa acgtgaaatg gatatgttca
     4981 cggtgaatgc atatgaaggc gccattcagt ttccattgac gattataccc gttgatgtgc
     5041 agggcgatgt tgcgcttaaa ctgggcttga agctgatggt atgcaaggat atttgcattc
     5101 cgagcacatt ggatctggcg ctggatttga aacctgcttg gccagcccct taagctttag
     5161 aaatttcctc gtaaattttc agtgtgtgtg caacgacact ttcgacgtca aagttacgtg
     5221 cggccagatc atggctggca cggcccattg cttctaagca agggcggtct tcaagcagca
     5281 cccgcatcgc atcagccgtg gccagcgggt ctttgaccgg caccaataga ccattcactt
     5341 catgctcgac cgcctcgcgg cagcccgggt gatcggttgt gacaatcgcg cggcctgtgg
     5401 cggcggcttc cagtaaaact ttcggcacac cttcgccata ccaggatggg tagcaaatca
     5461 ggctggcgga ttggtaaagt gcaggcatat cggcgacttt gccaagccat tccacctcac
     5521 tttcagccag catcgtttcc atttcttctt ttgaaattgc gctgggggtt gtggcactca
     5581 atcctccggc aattttaaac cgtgcattta ttccacgttt tttgagaata ttggccgcct
     5641 tgataaacac cgcgacgcct ttttcatgga taaggcgtgt gggcatgagc acgattgggc
     5701 gcttgtcttc cggcagcggc atcaacggat attgcgttaa atcaacgccc gaaccgcgaa
     5761 taagtgtaca gcgttcggga acggcaaatc tacggcggat catcagtgcc atatcatcgg
     5821 ggttttgaaa gatcagatgc gtgcgttttt gtttgagggc taattttagg aacgggccaa
     5881 tcgctatacg caaaatttgc ggctttgcac cttcgccgga aaacagataa cccagtccgg
     5941 ccagcgtatg gacgacccga acgtctttat aaagccttgc ggcgagaccg gcgaaaaatg
     6001 catatttcag tgtgatggca tggatgatcg ccggatgttc gcgcttaata attttgtaga
     6061 tagcccagat gattttggcc gggccggatg cggggagttc atgtcctgtg aaatgttgcg
     6121 gggcgaggtc gggatcgcta tccgcgccgg tcacagcaac gctgacgtcc cagcctttaa
     6181 cttgcgcgcc tttggccagc ggtagacgat gcgaccaaaa ccagtcaatg tgattgataa
     6241 cgagaagaag ttttttggct gccatttatg cagccctttt atgatcttgt tttttatcct
     6301 gaaattcgca ttcaatcatt tcttccagcc cgtcatgaag ggaataaggc gctttaaaac
     6361 cggtttcttc tttgagctta tcggcattta caatcgtatc ggcgcagaat tttttgatac
     6421 ggataatgct gattggaaat tttttgccag tgactttcgc cgcgacatca aacacgctgc
     6481 cgccgagtag acccagcgca taagacattt gcgggccgag gcccctatag cccagagctt
     6541 tgcggatgga agcaacaaaa tcgcgcatat ttatatccgg cttatcggcg tagttataaa
     6601 gatgcgcacc cgctttaaag cccaaagcat ggtgaaggaa ggctgcgaca ttgccaacat
     6661 aggcgatgga tttacagttc tttccatcgc cgatcataat gaatttgccg ctggcgatct
     6721 gatttatcag ggtaaagatg ttgccgcgat tgccggggcc aaaggtggcg acaaggcgga
     6781 tgatggtcag actgcgggcc gggtcttggg aggcccaata ttcgaaggtc ttttcgctgt
     6841 cacatttcga ttgcccgtat gggttaaagg gggcgggcgg atgctcttcg gaggagcctt
     6901 tttttagatc ggccggaacg agtggataga ccgcgaccga gctggtgaag ataatttttt
     6961 taatgccatt agccttggca gcctcgatca cgttttggcc gccggtaaca ttgacatcgg
     7021 tgtagagaga aagcggagtc acgtcatcgc ggtgctcggc ggcaaggtgg tagatggcgt
     7081 ctgcaccttc caaggcttta gtgagggtgt ctaaatcccg aatatcggca atgatgctat
     7141 catccggaaa actttccgat tttttgaggt cgatcaaaac caattcatgg ccgtcacttt
     7201 tcagtagctt tgccagttgc gtcccgataa accccgcccc acctgtaatt gctattttca
     7261 tggcctctgt tctacctcac atccagcgtt tatgccaggc ctgaaacatt aaaatacacc
     7321 aaagcgcttc gccatgcgca ccctgcgcgc ccagatgcga ctgccagagt ttccgcgttt
     7381 tttgtacatc aagcagccct tcttcgcgca gatgtttttc atcgagcagg ctttcggccc
     7441 aatcgcgtaa atcaccgcgc agccaatcac cgaccggcat gttaaaaccc tgcttggggc
     7501 gctgaaaaag tttttcagga acgtgccgtt ttagaacctg gcgcaacagc catttgccct
     7561 gcccgcctcg aattttgata ttttccggca aggaccagac ataatcgaag atccgtgtat
     7621 cgagcaacgg cgcgcgcgct tccagaccat aggccatgga tgcccggtca actttggtga
     7681 ggatgtcgtg tggcaaatag
//
LOCUS       DAOSWR010000099         7669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6162_length_7669_cov_5.540977, whole genome shotgun sequence.
ACCESSION   DAOSWR010000099 DAOSWR010000000
VERSION     DAOSWR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_7669_cov_5.540977"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..735
                     /locus_tag="PK513_07680"
     CDS             313..735
                     /locus_tag="PK513_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82365.1"
                     /translation="MMSKVSQAFGVLALSAASAVYAQGVAASEQVCAKDGRSFEITEN
                     NGQITGYYHQNRQNISSETLANFQSRSSSAGTRSVGTGGNPRTAPFKEAFGFSRGQAN
                     LPSPDERIEALRTRGKIFCYTGHVPTPAPGSFVPLKPQ"
     gene            complement(737..2527)
                     /gene="sdhA"
                     /locus_tag="PK513_07685"
     CDS             complement(737..2527)
                     /gene="sdhA"
                     /locus_tag="PK513_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOO82366.1"
                     /translation="MTNEYEIIDHEYDVVVVGAGGAGLRAGFGCAEKGLNTAIISKVF
                     PTRSHTVAAQGGISAALGNMGEDDWRFHMYDTVKGSDWLGDQDAIEYMCREAIPAIIE
                     LEHYGVPFSRTAAGKIYQRAFGGMTTHYGKGTAQRTCAAADRTGHAILHTLYTQALKH
                     KAEFFIEYFALDLIMSDDGQCLGVMAWNLDDGTIHRFRAHQVILATGGYGRTYFSCTS
                     AHTCTGDGNAMALRAGIPLQDMEFIQFHPTGIYGAGCLITEGVRGEGGYLTNSEGERF
                     MERYAPSAKDLASRDVVSRSMTIEIREGRGVGEHKDHIHLHLEHLDSDIIHERLPGIA
                     ESARIFAGVDVTKEPIPVLPTVHYNMGGIPTNYMAEVINPTKKDPNAIVSGLMAIGEA
                     ACVSVHGANRLGSNSLLDLVVFGRAAANRCAETVVPGAKHRPFGTDDGSKALARLDHY
                     RSAKGSTTTAALRDRMQRTMQTHAPVFRTGETLNEGITKIDECFAGKADLKVTDRSLI
                     FNTDLVETLELDNLLSQAVAAMHAAANREESRGAQAREDFPDRDDKKWMKHTVIWVDE
                     AGKTKIDYRPVIMTTLTDEVQPIPPKARVY"
     gene            complement(2529..2855)
                     /locus_tag="PK513_07690"
     CDS             complement(2529..2855)
                     /locus_tag="PK513_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82367.1"
                     /translation="MTEFYNPIEKYNKQDRKIFGFLDKADNILNYITFMVTGVIAGAI
                     LGLFIGKISIYWYLAGGLIAGVGFGKGNIWMKKTFDDDIPIIPVLLLICLIIVGCLYV
                     VSWMVI"
     gene            complement(2952..3347)
                     /gene="sdhD"
                     /locus_tag="PK513_07695"
     CDS             complement(2952..3347)
                     /gene="sdhD"
                     /locus_tag="PK513_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOO82368.1"
                     /translation="MNMKWDNHGMKTPLARARGLGASHAAVPGWIRLRVTAIGTAVLS
                     LWFLWFLSQTIGTSHAEFTAKLADPRNAICMLLLIIAAFTHAVLGNREIVEDYIHNEF
                     FKLSKLVGMYLFFFALATACIFSILKIAL"
     gene            complement(3344..3808)
                     /gene="sdhC"
                     /locus_tag="PK513_07700"
     CDS             complement(3344..3808)
                     /gene="sdhC"
                     /locus_tag="PK513_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOO82369.1"
                     /translation="MSQSAPTKTQRPLSPHLQVYKPQMTSVLSILHRMTGVGLTVGLM
                     LFAWWLVAAAIGPGAYAVFMNFAGSPVGMLLLFGFTLAFFYHLANGIRHLFWDSGLLF
                     KIENAYKAGYAVLLFTALMTAATWYCVLNKGPYASPSVTATLTAAEAEGETR"
     gene            complement(3971..4489)
                     /gene="msrB"
                     /locus_tag="PK513_07705"
     CDS             complement(3971..4489)
                     /gene="msrB"
                     /locus_tag="PK513_07705"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOO82370.1"
                     /translation="MHRRAFLAGVACAALMTGASKAMAFDIFSKNKSLPHSSRKGDFP
                     FQLTDEEWRARLSPEQYEILRGEGTEKSCSSPLNGIEGHGKFYCAGCGHHLFSTDAKY
                     DSKTGWPSFYQPADEYAVGTSTDYKLGYARTEVHCANCGSHLGHVFKDGPPPTGLRYC
                     INGLALEYQQEQ"
     gene            complement(4554..5576)
                     /locus_tag="PK513_07710"
     CDS             complement(4554..5576)
                     /locus_tag="PK513_07710"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO82371.1"
                     /translation="MGYKVAVVGATGNVGLEMLKILDERDFPADDVVALASERSVGRE
                     VSYGDTDLRVQNLADFDFKGVDIVLSSPGAKVSAEYSPKAAAAGATVIDNTSYWRMDP
                     DVPLIVPEVNPHAINEIKKGIIANPNCSTIQMVVALKPLHEAATIKRVVVSTYQSTSG
                     SGKAAMDELFNQTKGIYANQTPTPSVYPKQIAFNAIPQIDVFMDDGMTKEEWKMVVET
                     KKILDPAIKVCASCVRIPVFIGHAEMINVELEKEISAIEAKELFHNAEGVTLVDLEQE
                     DLEYVTPAEIQGEDDVFVSRVREDITVENGLNFWCVADNLRKGAALNTVQIAELLVKN
                     EAQKAA"
     gene            5658..7625
                     /locus_tag="PK513_07715"
     CDS             5658..7625
                     /locus_tag="PK513_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82372.1"
                     /translation="MIDSSKLSRSELEQKIAEADALAEMKSNFLATMSHEIRTPMQAI
                     FGLLELISDEKPSENISEMVRTAQGAAAGLLEILDDILDLAKMDADKMELDMFEVPVR
                     LLVRGLLEALAVKVHGVDIELIDDIEQDVPFVVVGDPKRLRQIIMNLCGNALKFTHQG
                     SVTVRVTSQVQAIDVPEGGVGLRFEVIDTGIGMSQEVCARLFGSFVQADNSTSRKYGG
                     TGLGLSISKKLVELMGGRIGVNSAPGKGSTFWFEIPTQEVSTDDSTVELPSLDGVSVL
                     SVEDHPQGAKEIVNSLRSMGASVENCPTYKEGLELITRRPFDVAVIDQGLPDGLGLDL
                     IKEITKLRPNTGLVMYTVRDDVGLSHSLKALGVTYLTKPASRIGLGEAVRDAASRLEH
                     IEITGPTKLLIAEDTESVRDILGRQLDKLGVEASFVENGRLALEALETGEYGLLITDL
                     HMPEIDGYGVVEHIRKQEKEAGDGTHFPVMVLTADVQMAQRETYMRHGFDECLLKPVS
                     LGQFKRLLIRWGLLNEETGDDSLGAVPVNAPKEDEGGLPPAIDKDAMIEQMGEFDEMA
                     VEMLGMFVDMTRPQIDKIKSALAAEDFKDLCELGHSLKGAARSACCPALGDLASQLQD
                     DADVQKPVADLVAEILKEFKRVEAEISALQK"
BASE COUNT         1796 a         1879 c         2144 g         1850 t
ORIGIN      
        1 tgaaaaatga attagccaag aagcaggctc taaaagaatt ccgccccaaa ccgttcttac
       61 ggcggaattc ttttagagcc tgcttcttgg ctaattcatt tttcaaactc atggccgaaa
      121 cattatagta atttacatat aaaatcaagc ttttcagcgt taagcgccat aaatgtttat
      181 tcctgtttat gttgggtcaa agagtcctta cgggaaattc actctgggca accactgcat
      241 tttacataat tttcttgtgt gcgtagcttg attcatgtat tgtattgtca cattcaagtt
      301 tatgcagggg gtatgatgag taaagtttca caggcatttg gtgttttggc gctcagcgca
      361 gcttcagcgg tttacgcgca aggggtggca gcaagcgaac aggtttgcgc gaaagatggt
      421 cgtagctttg aaattacgga gaataatggt cagattactg gttattatca tcaaaataga
      481 caaaatattt catcggaaac cctagccaac tttcaaagca ggagctcttc agccggaaca
      541 cgatcagtag gcaccggcgg caaccctaga accgcaccgt tcaaagaagc ttttggattt
      601 agtcgggggc aggccaacct tccttctcca gatgaacgaa tcgaagccct gcgcacacgc
      661 ggcaagattt tctgctatac aggccatgtt ccaacccctg ccccaggcag ttttgttcct
      721 ttgaagcccc aataacttaa taaacccggg ccttgggcgg gatgggctgc acttcgtcgg
      781 tcagggtggt catgatcacc gggcggtagt caatcttggt tttcccggcc tcatccaccc
      841 agataacggt gtgtttcatc cattttttat catcgcggtc ggggaagtcc tcacgggcct
      901 gcgcaccgcg ggattcttcg cgattagcgg cagcatgcat tgcggcgacg gcttgggaca
      961 agaggttatc cagctccagc gtttcaacga gatcggtgtt aaagatcaac gagcggtcgg
     1021 tgactttcag atcagccttc ccggcgaagc attcatcaat cttggtgatg ccctcattca
     1081 gggtttctcc ggtgcggaat acgggcgcat gcgtctgcat cgtgcgctgc atgcggtcgc
     1141 gcagcgcggc tgtcgtcgtt gaacctttag cgctccgata atgatcaagg cgtgccagcg
     1201 ctttcgagcc gtcatccgtg ccaaacgggc ggtgctttgc gcccggcaca acggtttcgg
     1261 cgcagcgatt ggctgcagcc cggccaaaga cgacgaggtc gagcagtgag tttgagccca
     1321 gacggttggc gccatggacg gagacgcacg cggcttcgcc gatcgccatc aagcccgaca
     1381 caatggcatt gggatctttt ttggtcgggt taatgacctc ggccatgtaa ttggttggga
     1441 tgccgcccat attgtaatgc acagtcggca gaacagggat cggctctttg gtcacatcta
     1501 cgccagcgaa gatgcgggcg ctttcggcga tacccggcag gcgttcgtgg ataatgtcgc
     1561 tgtccagatg ttcgaggtgg aggtggatgt ggtctttatg ctcaccgacg ccgcgccctt
     1621 cgcggatttc gatggtcatc gagcgtgaaa caacgtcgcg cgaagcgagg tcttttgcgc
     1681 ttggggcata acgttccata aagcgttcgc cttcggaatt ggtcagatag cccccttccc
     1741 cgcgcacacc ttcggtaatc aggcaacccg cgccgtaaat tccggtcggg tggaactgga
     1801 tgaattccat gtcctgcaac ggaatgccgg cgcgcaaggc catcgcattg ccatcgcccg
     1861 tacaggtgtg ggcggaggtg caggagaaat aggtgcggcc ataaccgccg gtggcgagaa
     1921 taacctgatg cgcgcggaag cggtgaatgg tgccgtcatc gaggttccag gccatgacac
     1981 cgaggcactg gccgtcatcg ctcatgatca ggtcgagggc aaaatattcg ataaagaatt
     2041 cggccttgtg tttgagggcc tgcgtgtaaa gcgtgtggag gatggcgtga cctgtgcggt
     2101 ccgcggcggc gcaggtgcgt tgggcggtgc ccttgccgta atgcgtcgtc atgccgccaa
     2161 aagcacgctg gtaaattttc ccggcggcgg tacgggaaaa cggtacacca tagtgttcaa
     2221 gctcgataat cgcggggatc gcctcgcggc acatatattc aatcgcgtcc tgatctccga
     2281 gccaatctga gcctttaaca gtgtcataca tatggaagcg ccagtcgtct tcgcccatat
     2341 tgcccagcgc tgcggagatg ccgccttgcg ccgcgaccgt gtgcgagcgg gttggaaaga
     2401 cctttgaaat gatcgcggtg ttcaaacctt tttctgcgca gccaaagccg gcgcgcagcc
     2461 ccgcaccacc ggcgccgacc accacgacat catattcgtg atcaatgatt tcgtattcat
     2521 ttgtcatttt atattaccat ccatgaaaca acgtaaagac accctactat aatcagacag
     2581 atcagcagta aaaccggaat aatgggaata tcatcatcga atgttttttt catccagata
     2641 ttacctttgc caaaaccaac ccctgcgatc aggccacctg ccaaatacca atatatagag
     2701 atctttccta taaacaggcc cagtattgcg ccggctatta cacctgtgac cataaaagtt
     2761 atatagttaa ggatattgtc agccttatca agaaacccga aaatttttcg atcttgttta
     2821 ttatattttt caatcgggtt gtaaaattca gtcatttctt tcccttttta cgcggagaaa
     2881 cggaggaacg gaggtttatt ttttataata cacaaaactc tgaaactccg aatctccgcg
     2941 tttaattttt tttacaacgc tatttttaga attgaaaaaa tgcaggcggt ggcgagggcg
     3001 aaaaagaaca gatacatgcc aaccagcttg gatagcttga aaaattcatt gtggatgtaa
     3061 tcctcgacga tttcacgatt tcccagcacg gcgtgggtaa aagcggcaat gatcagcagc
     3121 agcatgcaaa tcgcgttgcg cggatcggca agcttggctg taaactctgc atggctggtg
     3181 ccgatggtct ggctgagaaa ccacaggaac cacaatgaga gcactgcggt gccgatagcc
     3241 gtgacgcgca gcctgatcca tcccggcaca gccgcgtgcg atgcgcccag accgcgggcg
     3301 cgggcgagcg gggttttcat accgtgattg tcccatttca tattcatcgt gtttctcctt
     3361 cagcttcggc ggcagtcagc gtcgcggtga cagacggcga tgcgtatggg cctttattca
     3421 aaacgcaata ccatgtcgcg gcggtcatga gcgcggtgaa cagcaaaacg gcgtagccgg
     3481 ctttataggc gttttcgatt ttaaacagca atcctgagtc ccagaaaaga tggcggatgc
     3541 cgttggccag atggtagaaa aatgcgagcg tgaagccgaa cagcaagagc atgccaacgg
     3601 gcgagcctgc gaagttcatg aatacagcgt aggctccggg gccgattgct gcggcgacca
     3661 gccaccaggc aaacagcatc agaccaaccg taagaccgac ccctgtcatc cggtgcagaa
     3721 tcgataaaac agaggtcatt tgcggtttat aaacctgaag gtgcggggat aatgggcgct
     3781 gagttttggt gggggcgctc tgtgacatga tgtttccttt tgaattcttc gaacaggtca
     3841 agaaacatca taaaacgccg ggcgggtttt gcaatggttt aatcacaccc aaatcccctc
     3901 acccagaggt gccatctggg aacacttact tttagggcaa aaactaagta tccccagaca
     3961 gagacaaagg ttattgctct tgctgatact ccagcgccag accgttaatg cagtaacgta
     4021 ggccggttgg cggcggaccg tccttgaaaa catggccgag gtgagagccg caattggcgc
     4081 aatgaacctc ggtacgggca tagccaagct tgtaatccgt ggaggtgccg accgcgtatt
     4141 catcggcggg ctggtaaaaa ctcggccagc cggttttact atcgtatttt gcatcggtac
     4201 tgaacaggtg gtggccgcaa ccggcgcaat agaacttgcc gtggccttcg atgccgttta
     4261 gcggcgacga gcaggatttt tccgtgcctt cgccgcgcag gatctcgtat tgttcggggc
     4321 tgaggcgcgc gcgccactcc tcatcagtaa gttgaaaggg gaaatcacct ttcctgcttg
     4381 agtgtggcaa agacttgttt tttgaaaaaa tgtcaaaggc catcgctttg cttgctcccg
     4441 tcatcagggc ggcacaggcc accccggcta aaaacgctcg tctgtgcatg cttaaacatt
     4501 ccctattgtt catacagggg tattcgcatt ttaaagcaaa aaggttacag gtcttaagcc
     4561 gccttttgcg cttcgttttt caccagtaat tcagcgattt gcacggtgtt gagcgctgcg
     4621 cctttgcgca aattatccgc cacgcaccag aaattgaggc cgttttcgac cgtgatgtct
     4681 tcgcgaacgc ggctgacgaa cacatcgtct tcgccctgaa tttcagccgg ggtgacatat
     4741 tcgaggtctt cctgctccag atcgaccaac gtaacccctt cagcattatg gaagagttcc
     4801 ttggcctcaa tggcggaaat ttccttttcc agctcgacat tgatcatttc ggcgtggccg
     4861 ataaacacag ggatgcgtac acaagaggcg caaaccttga tcgcgggatc gagtatcttt
     4921 ttggtctcaa ccaccatttt ccattcttcc ttggtcatgc cgtcatccat gaacacgtcg
     4981 atctgcggga tggcgttaaa ggctatctgt ttcggataga cagacggggt tggcgtctgg
     5041 ttggcgtaga tgcctttagt ctggttgaac agctcatcca tcgccgcctt gccggaaccg
     5101 gaggtggatt gataggtact gaccacgacg cgtttgatgg tcgcggcttc atgcaaaggc
     5161 ttgagcgcga caaccatttg aatggtggaa cagttcgggt tggcgatgat gcctttttta
     5221 atctcgttga tcgcgtgagg attgacctcc ggcacgatca gcggcacatc cgggtccata
     5281 cgccagtagg acgtgttatc gatgactgtc gctccggcag cagcagcctt aggcgaatat
     5341 tcagccgaga ctttagcacc gggtgaggac agcacgatat ccacaccttt gaagtcaaaa
     5401 tcagccagat tctgtacgcg caaatccgta tcaccatagg acacttcgcg ccctacagac
     5461 cgctcggagg ccagcgctac aacgtcgtca gcaggaaagt cgcgttcatc caatattttt
     5521 aacatttcaa gcccgacatt acctgtggcg cctacaactg caactttata tcccattttt
     5581 gtttcttcct gctttgagcc ttcattttag aggatttcat tgatatgata tgtgggcatt
     5641 caaatgcaag gtttttcatg attgattctt caaaattatc ccgttctgaa ctcgaacaaa
     5701 aaatcgccga agccgatgct ttggcggaga tgaagtcgaa ttttctggcg acgatgagcc
     5761 atgagatccg tacgccgatg caggcgatct tcggtttgct ggaactgatt tccgacgaga
     5821 aaccttcgga aaatatttcc gagatggtga gaaccgcgca aggcgcggct gcgggcttgc
     5881 tggaaattct cgatgacatt cttgatctgg cgaagatgga tgccgacaag atggagctcg
     5941 atatgttcga ggtgccggtg cggctcttgg tacgcggatt gctcgaagcg ctggctgtga
     6001 aagtccatgg cgtggatatt gagctcattg atgatattga acaagacgtg ccgtttgtgg
     6061 tggtgggcga tccgaaacgg ctgcgccaga tcattatgaa tttgtgcggc aatgcgctta
     6121 aattcacgca tcaaggcagc gtcactgtgc gggttacgtc gcaggttcag gcgattgatg
     6181 ttcccgaggg cggtgtgggc ttgcgttttg aggtgattga taccggaatc ggtatgagcc
     6241 aggaggtctg cgcgcggttg tttggatcgt tcgtgcaggc ggataattcg accagccgaa
     6301 aatatggcgg cacggggctg gggctgtcga tttcgaaaaa gctggtggag ttgatgggcg
     6361 ggcgaatcgg tgtgaacagc gcccccggga aaggctcgac cttctggttt gagattccaa
     6421 cgcaggaagt gagtacggat gacagcacgg tggagttgcc ttcactggat ggggtttccg
     6481 tgctttcggt tgaagaccac ccgcaaggtg ccaaggaaat tgttaattcg ctgcgctcta
     6541 tgggggcgag cgtcgaaaac tgcccaacct ataaggaagg gctagagctc attacccggc
     6601 gtccgtttga tgtggcagtg attgaccaag ggctgcccga cgggttgggg ctggacctca
     6661 tcaaagagat taccaaactg cgcccgaata cggggcttgt aatgtatacg gtgcgcgatg
     6721 atgtggggct gtcgcattca ctgaaagctt tgggcgtaac atatctgacc aagccggcca
     6781 gccgaatcgg gctgggagag gcggtgcgcg atgcggccag tcggctcgag catattgaaa
     6841 ttaccgggcc gacaaagctg ttgatcgccg aggacacgga aagtgtacgc gacattctgg
     6901 gccgtcagct tgacaagctg ggcgttgagg caagttttgt tgaaaacgga aggctggcgt
     6961 tggaggcttt agaaaccggc gaatacggcc ttctgatcac cgatttgcat atgccggaaa
     7021 tagatggcta tggcgttgtg gagcatattc gcaagcagga aaaagaggcc ggggacggca
     7081 ctcatttccc tgtgatggtg cttacggccg atgtgcagat ggcgcagcgc gagacttata
     7141 tgcgtcacgg gtttgacgaa tgtttgctca agcccgtttc attgggacag tttaagcggc
     7201 tgcttattcg ttgggggctt ttaaacgaag aaaccgggga tgattctctc ggcgctgttc
     7261 cggtgaacgc ccccaaagaa gatgagggcg gcttacctcc cgcgattgat aaagacgcca
     7321 tgatcgagca gatgggggag tttgatgaga tggccgttga aatgctggga atgtttgttg
     7381 acatgacacg cccgcaaatt gacaagatta aaagcgcact ggccgcagaa gactttaagg
     7441 atttatgtga gttggggcac agcttgaaag gcgcggcgcg cagcgcgtgc tgcccggcgc
     7501 tcggggatct ggcatcgcaa ctgcaggatg atgcggatgt acaaaagccg gttgcagatt
     7561 tggtggcaga aattttaaaa gaatttaaac gagtcgaagc agagatttca gcgctacaga
     7621 aataatcaat atttcgagcc taaacgtttt aaggacaccc gccctcatc
//
LOCUS       DAOSWR010000100         7660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6173_length_7660_cov_5.789218, whole genome shotgun sequence.
ACCESSION   DAOSWR010000100 DAOSWR010000000
VERSION     DAOSWR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6173_length_7660_cov_5.789218"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="ftsZ"
                     /locus_tag="PK513_07720"
     CDS             complement(<1..817)
                     /gene="ftsZ"
                     /locus_tag="PK513_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO82373.1"
                     /translation="MAINISMQPTEVQKLSPRIIVAGMGGAGGNAVNNMISSGLEGCE
                     FLVCNTDAQALENSMCNRKVQLGVGVTGGLGAGSKPEVGKAAAEESIEEVMQYFEGAN
                     MAFITAGMGGGTGTGAAPVIAKACRERGILTVGVVTKPFHFEGSHRMKSADAGIEEIQ
                     DYVDTLIVIPNQNLFRVANEKTTFADAFQMADSVLQAGVRGVTDLMVRPGLVNLDFAD
                     IRTAMLEMGKAMMGTGEAEGDKRAIEAAESAINNPLLDDVSMKGAHGVIINVTG"
     gene            1207..2019
                     /locus_tag="PK513_07725"
     CDS             1207..2019
                     /locus_tag="PK513_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82374.1"
                     /translation="MSKIHIPNGAPTQHTEEEISSLVDVFTAGKVRAAQEDRLLVIFN
                     GTVGAGKTRAMNHYFHDLGPEEKSQTVYCAYDEHGLLFAMKKYRDEIKALRRKHNGKL
                     PHKERMEIRDKYQLDTQYGFALVQQKAIEQGNSLFIDMTMQSKHALSALEPYKKDGRK
                     IILESYISPREIAKERVNRRIRSLWPDEFDKKYDGWFGNFANLVDNGLAIHLHHNPED
                     GKPPLLVASFNNGAAMDINNEHYTRMVEDLQKQKGQTQIFLKKIEELVNAYS"
     gene            complement(2026..3312)
                     /gene="ftsA"
                     /locus_tag="PK513_07730"
     CDS             complement(2026..3312)
                     /gene="ftsA"
                     /locus_tag="PK513_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOO82375.1"
                     /translation="MTRHKHKSQKHKPKGTLLAALDIGSSKIACFIARVVDDEGTFEV
                     LGVGHHASKGIKNGTIVDLDAAESVIRQTVHAAENMAADVMKGYPLREVVVNLPGVQA
                     ASHAYTSDVQIAGHEVTDNDVRRALAKAQEQVLSEDYELIHTIPMDFRIDGNEGIRDP
                     RGMFGQDMSVDIHMVTGDMGPLQNMATAIERSHLDITALCVSAYAAGLAALVEDEMDL
                     GCTVIDIGGGVTSFAVFNGGRMVYADAVPLGGEHITGDIAKGLTTSLHDAERLKALYG
                     SAMASDMDETELIDVPRLGEDDHHEPNHVPRSLLVGIIQPRIEEIFEMVRAKLNDSGL
                     GNAIGRRVVLTGGSSQIQGLKELAGQVLDKQVRLGKPIRLTGLPDAVSGPSFATTAGL
                     LTYISERVDEMPAQIMAGAETGSLWERVRFWLKENW"
     gene            complement(3312..3782)
                     /locus_tag="PK513_07735"
     CDS             complement(3312..3782)
                     /locus_tag="PK513_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOO82376.1"
                     /translation="MKERRPLALWQSEKRLKLLDDKGKVIVTSNLQRFADLILVIGKD
                     SPVATPELIGNLQAEPDIYKRVEMAKYISQRRWDLQLKNGIEVKLPEEDMGLALARLA
                     RAQEKDGLLDKDIRSIDMREIDRISVRTKPGKVQEYKTDLKIEAATKGKAGNNI"
     gene            complement(3789..4199)
                     /locus_tag="PK513_07740"
     CDS             complement(3789..4199)
                     /locus_tag="PK513_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOO82377.1"
                     /translation="MASKNKKGTRRSAINKRRKGLLAVVLPWLRRFGLVAAVFIGLIW
                     IGSWLYLSGAVGRAGDWSRDKILTASADMGFAIENILVDGRRYADAHVLLAIVNVEKG
                     DPLFSFDPRVAQELISQVGWVESVQVERRLPSTI"
     gene            complement(4203..5435)
                     /locus_tag="PK513_07745"
     CDS             complement(4203..5435)
                     /locus_tag="PK513_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO82378.1"
                     /translation="MTQNKKTVAVFFGGRSPEHDVSVVTGLQILSAIDAQSFEAFPVY
                     ITTEGEWLVGDLLKDRANYMLSPQARKEVQAVTLDLKPGRRAQLVPLKTGLFGGAKPL
                     EFDVVIPSFHGLFGEDGNIQGLFEFAGVPYAGMRTMASSILMDKGATKKVLQGASIPV
                     LPYAMIYRPEQGYMIEEDALKTMMGEVEFPCIVKPSHLGSSIGVAKAGNIEDLRASLP
                     SIFEYDDAAIIEPFVENLVEYNVAVSKAFGEIRTSAIERPKATEELLDFKQKYLSGKD
                     SKTGQKLDGDEGGTKTPGTISQGMLSLTRELNPKLKPQQVKNIKDWALTMFKAVEGTG
                     APRIDFIGNEKTGELWLNEVNPWPGSIGYFLWEAAENPVLFTDLLTGLIEEALKENKK
                     RSLPKDPVPVDARLLRRA"
     gene            5578..6336
                     /locus_tag="PK513_07750"
     CDS             5578..6336
                     /locus_tag="PK513_07750"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO82379.1"
                     /translation="MSQDIYFEKMGKGGPPVFWGHGWGQNLHALRPLADTLSNMGQHW
                     VVDFPGFGKSPLPGEVWGTEEYANAMAALIRAQTDGPVIWAGHSFGCRVGLQLAARHP
                     DLIAGLVLIAGAGLPRKRPLWHKLYYGARVKLFKLLKKLIPLGLSQDWLYEKFGSADY
                     KSAGPLRQIFVKVVNEDLSDVAAQVKCPTLLIYGSKDTETPPEIGQRLSRIIPGAKLI
                     LMDGFDHYSILTGARHQVVRHIHNFMKEISKHDD"
     gene            6326..>7660
                     /locus_tag="PK513_07755"
     CDS             6326..>7660
                     /locus_tag="PK513_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOO82380.1"
                     /translation="MMIELATTALLFLSFLAFAAKRGLTYMHVYQQEEYDSERLLSWI
                     WKNKAFDKRLSVVVIVVAILSFFIPPVFVNFLIFTAMAFATYREKDPRKDSKKKLVAT
                     ARAKRIFFPAYFLSVLAATWCFLPVTPMPWLWILNIHLIPFMILFVNMMLAPFEEMNQ
                     KKFWTEAHNKVLDYQPTVIGITGSFGKTSVKHILGHILKTQAPTLVTPGSVNTPMGIS
                     RILREQLEPEHKYLIVEMGAYGRGSIDRLCKLTPPDFGIITAIGHAHYERFKSLDTVA
                     ETKYELAESVVSRGGQVVVHERTLRFDYTRAMKEQNENHFIVCGESADVDKSKQTDKS
                     YLKKGALEIHKIIQGGNGIEVRFTWKNVTYNVEAPLFGIHHGHNIAMAFVAAFELGVS
                     SKDIQAALQSLPQIEHRLEVRKQAGGLTIIDDAYNSNPMGFQAALGLLSQIGS"
BASE COUNT         1906 a         1902 c         1902 g         1950 t
ORIGIN      
        1 cgccggtgac gttgatgatg acgccgtgcg cgcctttcat tgaaacatca tcgagcagcg
       61 gattgttaat ggccgattcg gcagcttcga tggcgcgctt gtcgccttct gcctcacctg
      121 ttcccatcat ggccttgccc atttcgagca ttgccgtgcg gatgtcagca aagtcgagat
      181 tgaccagacc aggacgcacc ataaggtctg ttaccccacg cacaccagct tggagtacag
      241 agtcggccat ctgaaatgca tcagcgaagg ttgttttttc attggcaacg cggaagagat
      301 tctgattcgg aattacaatc agcgtatcga cataatcctg aatctcttcg atcccggcgt
      361 cagctgattt catacgatga gagccttcga aatggaatgg tttcgtgaca acgcccacag
      421 tcagaatgcc tcgctcgcgg caagctttgg caatcaccgg cgcggcgcct gtgcctgtac
      481 cgccgcccat accggcggta ataaaagcca tgttggcgcc ttcaaaatac tgcattacct
      541 cttcgattga ttcttcggca gcagccttac caacttcggg ctttgagccc gcacccaatc
      601 caccagtgac gccgacaccg agctgaacct tgcggttgca cattgaattt tcaagcgcct
      661 gtgcgtctgt gttacacacc aaaaattcgc acccctcaag cccggaggag atcatattgt
      721 tcaccgcgtt tccgccagca ccacccatgc cggccacaat aattcttggc gagagtttct
      781 gaacttccgt cggttgcatt gagatgttta tagccatggt gaatatcccc aatatgaacg
      841 ttttgcgccg ctgatagaaa agctttgcga tatattacgc atgagtttag gcaagtttta
      901 aacatgcgaa aagataaaaa tgacaaggat tcacagggtt tgactgcgaa gagctgcatt
      961 tgagtctagc accgcttgag ataaaaatgc aaaaatgagt cttaagtgac tcatagatca
     1021 tcatttttta ttacaccccg cgcttcaccg cgctgtgcac aagttacgca cagaatgctg
     1081 tggataagtt taccctccga acatcctagc cattgccgcc ttccccttga ttcaaaaaac
     1141 ctataacttg cattgatttt tttattatgg taataagaat ttttctttaa aactattgag
     1201 gcgcaaatga gtaaaatcca cattccgaat ggtgcaccaa cgcagcacac ggaagaagaa
     1261 ataagcagtc ttgttgacgt ttttacagca ggcaaggtcc gcgccgctca agaggatcgc
     1321 cttttggtaa tttttaacgg cacggtcggg gcaggaaaaa cccgtgctat gaatcattac
     1381 ttccacgatt taggcccaga ggaaaaatct caaacggttt attgtgccta tgatgagcat
     1441 ggtctgcttt tcgccatgaa aaaatacaga gatgaaatta aggcattacg ccgcaaacat
     1501 aatggaaaac tgccccataa agagcgcatg gagattcgtg ataaatatca acttgatacc
     1561 caatatggat ttgcgctcgt tcagcaaaaa gctatagagc aaggaaacag cctttttatt
     1621 gatatgacca tgcaaagcaa gcatgcgtta agtgcgcttg agccttacaa aaaagacgga
     1681 cgcaagatta ttctagaatc ctatatatca ccgagagaga ttgctaaaga gcgtgtaaat
     1741 aggagaattc gcagtctatg gccagatgaa tttgataaaa aatatgatgg ctggtttggc
     1801 aattttgcta atctggtaga caatggccta gctattcatc tacaccacaa tccggaagat
     1861 ggaaagccgc cgcttttggt ggcgagtttt aacaacggcg cggcgatgga tattaacaac
     1921 gaacattaca cgcgcatggt tgaggatctg caaaaacaaa aagggcagac ccaaattttt
     1981 cttaagaaga ttgaggaatt ggtgaatgca tactcgtaga gcgcctcacc aattctcttt
     2041 cagccaaaat ctcacgcgtt cccagagcga accggtttcg gccccagcca tgatttgggc
     2101 gggcatttca tcgacgcgtt cggagatata ggtgagcagc ccggctgtcg tggcaaaaga
     2161 cggaccgctg accgcatcgg gcaggccggt caggcggatc ggtttgccga ggcgcacttg
     2221 tttatccaac acctggccgg caagttcttt tagtccttga atttgactgg aaccaccggt
     2281 gaggacgacg cgccgcccga tggcattacc caaaccgcta tcgttcaatt tggcgcgcac
     2341 catttcgaag atttcctcaa tccgcggctg aataatcccg accagcagcg agcgcggcac
     2401 atggtttggc tcgtgatgat catcctcgcc cagccgcggc acatcgatga gttctgtttc
     2461 atccatgtct gaggccatgg cgctgccgta aagggctttg aggcgctccg catcatgcaa
     2521 agatgtcgtc aggcccttgg caatatcgcc ggtaatgtgc tcgccgccca gcggaacagc
     2581 atcggcgtaa accatccgcc cgccgttaaa aactgcaaag gaggtcacgc cgccgccaat
     2641 atcaatgacc gtgcagccca gatccatttc atcttcgacg agagcagcca gtccggcagc
     2701 ataggcggaa acgcataggg cggtgatgtc gaggtgtgag cgctcaatgg ccgtggccat
     2761 attttgcagc gggcccatat caccggtgac catatgaata tcgacgctca tatcctgccc
     2821 aaacataccg cgtggatcgc ggatgccttc attgccatcg atacgaaaat ccatggggat
     2881 agtgtggatc aactcgtaat cctcgcttaa gacctgctct tgcgccttgg ccagtgcgcg
     2941 gcggacatca ttgtcggtaa cctcatgacc ggcgatctgt acatcagatg tataagcgtg
     3001 gctggcggcc tggacaccag gcagattgac tacgacttcg cgcaagggat agcccttcat
     3061 aacgtcggca gccatattct cggcagcgtg aacggtctgg cggattacgg attcggcggc
     3121 atcaagatcg acgatcgtac cgttttttat gcccttggat gcgtggtgac caacgccgag
     3181 cacctcgaac gtgccttcat catcgacgac gcgagcaatg aaacacgcta ttttgctgga
     3241 gccaatatcc aacgcggcca aaagcgtgcc ctttggtttg tgtttttggg atttgtgttt
     3301 atgccttgtc actaaatatt atttcctgct ttacctttag tcgcggcctc gattttaaga
     3361 tcggtcttgt attcttgtac tttaccgggc ttggtgcgta cgctgatgcg gtcaatttca
     3421 cgcatatcaa tgctgcgaat atccttatca agcaacccat ccttttcctg cgcacgcgcc
     3481 agacgcgcca gagcgagccc catatcctct tcgggtaatt tgacttcgat accatttttt
     3541 aattgcagat cccaacggcg ctgactgata tattttgcca tttcgacacg cttgtaaatg
     3601 tctggttcgg cttgcaaatt accgattagc tcaggcgtcg cgacgggtga atctttgcca
     3661 atcaccaaga tcagatcagc aaagcgctgt agattgctgg tgacaataac ctttccttta
     3721 tcatcgagaa gtttgagacg tttttcactt tgccaaagcg cgagggggcg gcgctccttc
     3781 aagccaatct agattgtact gggcaggcga cgctcgacct gtacgctttc aacccaaccg
     3841 acctgagaaa tcaattcctg tgcaactctt ggatcaaaag aaaaaagcgg atcgcctttt
     3901 tcaacattga cgatggcgag cagaacatgc gcatcggcgt atcggcgccc gtccacaaga
     3961 atgttttcaa ttgcaaagcc catatcagcg ctagccgtta aaattttatc gcggctccaa
     4021 tcacctgcgc gaccaacagc gccgcttaaa taaagccatg aacctatcca tatcaggcca
     4081 ataaaaacag cagcgacaag cccaaaacgc cgtagccatg gcagcacaac cgccaatagt
     4141 cccttgcggc gtttgtttat ggcgcttctg cgcgtgcctt ttttattttt agatgccatg
     4201 atttatgctc tcctcaaaag ccgtgcgtca acagggaccg ggtctttagg aaggctgcgc
     4261 tttttatttt ctttgagtgc ctcttcaatc agaccggtga gcaaatcggt aaacaagaca
     4321 gggttttcag cagcttccca gagaaaatag ccaattgatc caggccaggg attgacctca
     4381 ttgagccata gctcgccggt tttttcattg ccgatgaaat cgatgcgcgg cgcaccggtg
     4441 ccttcgacgg ctttaaacat ggtcagcgcc cagtctttga tgtttttgac ctgctgcggc
     4501 ttaagcttcg ggttgagttc acgggtgagc gagagcatgc cctggcttat agtgccgggc
     4561 gtcttagttc ctccctcgtc gccatccagc ttttgcccgg ttttactgtc cttaccggaa
     4621 aggtatttct gtttgaaatc gagtaactcc tctgtggctt tagggcgttc aatggccgag
     4681 gttctaatct caccaaaagc ttttgaaacg gctacgttat attcaaccag attttcaacg
     4741 aagggttcga taatcgccgc atcgtcatat tcgaaaatgc tcggcagact agcacgcaga
     4801 tcttcgatat tacctgcctt ggctacgccg atggaactgc ccaaatgtga aggtttgacg
     4861 atgcagggaa attcaacctc gcccatcatg gttttgagtg catcttcttc aatcatatag
     4921 ccttgttcgg ggcggtaaat catcgcataa ggcagaaccg gaatgctagc gccttgcagg
     4981 actttcttgg ttgcgccttt gtccatcaaa atggaagatg ccattgtacg cattccggcg
     5041 taaggcacac ctgcaaactc gaatagtcct tgtatattgc cgtcttcacc aaacagcccg
     5101 tgaaagctcg gaatgacgac atcgaactca agcggcttag caccaccgaa caacccggtt
     5161 tttaacggca caagctgggc gcgtctgccg ggttttaaat ccagcgttac ggcctgaact
     5221 tctttgcgtg cctgaggcga gagcatataa ttcgcgcgat ccttgagcag atcaccgacc
     5281 agccactctc cttccgtagt aatgtaaaca ggaaacgctt caaagctttg cgcatcaatg
     5341 gcacttaaaa tctgcaagcc ggtgaccacg ctgacatcat gttcgggcga gcgaccgccg
     5401 aaaaataccg caactgtttt tttattttgt gtcataagac cttacgggtt ggtttaaaaa
     5461 taaaaacttg ataagaacgt tagattgata ttaacagcat cgtcatttcg gtaaaagccg
     5521 gaatctcatg cttgtgaaca gcaagatccc ggcatccacc gggatggcgg cgggaatatg
     5581 tcacaggata tttatttcga gaaaatgggt aaaggtggtc cgcctgtatt ttgggggcat
     5641 ggctggggac aaaatctgca tgcgctgagg cctctggctg ataccctttc gaatatgggg
     5701 cagcactggg tagtcgattt tcctggattt gggaaatcac cgctgccggg tgaggtttgg
     5761 ggcacggagg agtatgccaa tgcgatggcg gcgctgatcc gtgcgcaaac agatgggccg
     5821 gtgatctggg ccggacattc tttcggctgc cgtgtaggtt tgcaactggc ggcgcgccat
     5881 cctgatttga ttgccgggct ggtcctgatt gccggagcgg gattgccgcg taaaaggcct
     5941 ttgtggcata agctgtatta tggcgcgcgg gtgaagctgt tcaagcttct caaaaaactt
     6001 attccgctcg gcctgtcgca agattggctc tatgaaaaat tcggcagcgc ggattataaa
     6061 agcgccggac ctttgcggca gatttttgtt aaagtcgtaa atgaagattt aagtgatgtg
     6121 gcagcgcagg taaaatgccc taccctgctc atttacggct cgaaagatac ggaaacaccg
     6181 ccggaaatcg ggcagaggct atcgcggata attccgggag ccaagctgat tttgatggac
     6241 ggatttgatc attacagtat cctgacaggg gcgcgccatc aggttgtgcg ccatattcat
     6301 aatttcatga aggagatttc caaacatgat gattgagctg gccactacgg ctctcctctt
     6361 tctgagcttt ctcgcttttg ccgcgaagcg tggattgacc tatatgcatg tttatcagca
     6421 ggaagaatat gacagcgaac gtttgctaag ctggatctgg aagaacaaag catttgataa
     6481 acgcttgagc gtcgtggtta tcgtggttgc cattctttcc ttttttatcc cgccggtttt
     6541 tgtgaatttt cttattttta ctgcaatggc gtttgcgact taccgggaaa aagacccgcg
     6601 gaaggattcc aaaaagaaac tggttgcaac ggcgcgggca aaacggattt tctttccggc
     6661 ttatttttta agtgttcttg ccgcgacctg gtgcttcctt cctgtgactc ccatgccatg
     6721 gctctggata ctcaatattc atctcatccc tttcatgatc ctgtttgtca acatgatgct
     6781 tgcgcccttt gaagaaatga accagaaaaa attctggacc gaggcgcata ataaggtgct
     6841 ggattaccag ccgaccgtga tcggcattac cgggtctttt ggtaaaacat cggttaaaca
     6901 tattctcggc catatcctga aaacacaggc tccgactttg gttacaccgg gcagcgtcaa
     6961 tacgccgatg ggcatttcac gcatacttcg tgaacagctt gagcctgagc ataaatattt
     7021 gatcgtggaa atgggtgcat atgggcgcgg atcgattgat cggctgtgca aacttacacc
     7081 gccggatttt ggcattatca ccgcgattgg ccatgcgcat tatgagcggt ttaaatctct
     7141 cgatacggtt gcggaaacta aatacgaact ggctgaatct gttgtaagcc gcggcgggca
     7201 agtggttgtg catgaacgaa ctttgcggtt tgattatacc cgtgccatga aggagcaaaa
     7261 tgaaaatcac ttcattgttt gcggagaatc ggctgacgtt gacaaaagca aacagactga
     7321 caaatcctac ctcaaaaaag gtgctcttga aattcacaag atcattcaag gcggtaacgg
     7381 gatcgaggtg cgttttacgt ggaaaaacgt gacctataat gtcgaggcgc cgctgtttgg
     7441 cattcatcac ggccataaca tcgcgatggc ctttgtggcg gcgtttgaac tgggcgtgtc
     7501 ctccaaagac attcaggcag cactgcaaag tctgccgcag attgaacatc gtctggaggt
     7561 gcgcaagcag gcgggcgggc taaccattat tgatgatgcg tataattcca acccgatggg
     7621 atttcaggcg gcgctcggcc ttctttcaca aataggcagt
//
LOCUS       DAOSWR010000101         7614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6227_length_7614_cov_5.683953, whole genome shotgun sequence.
ACCESSION   DAOSWR010000101 DAOSWR010000000
VERSION     DAOSWR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6227_length_7614_cov_5.683953"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..609
                     /locus_tag="PK513_07760"
     CDS             139..609
                     /locus_tag="PK513_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO82381.1"
                     /translation="MILQEKLQTLGLELPQAAAPAANYVPYVVADDDMLYIAGQIPFL
                     NGEQMHKGRLGEDLSVEQGVEAAQACALNILAQANAAVDGDWGKVKRLVKLGGFVNCT
                     PNFYDHPKVINGASDLMVKVMGEAGKHARFAVGAPSLPLGVSVEIDAIFELFRF"
     gene            complement(606..1448)
                     /locus_tag="PK513_07765"
     CDS             complement(606..1448)
                     /locus_tag="PK513_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82382.1"
                     /translation="MSEDSFINVLIAEDNEVSRDMMTGILRAQGYNVLGAVDGESAIR
                     VVDDNLVDIALVDINMAPTGGFEFMKYVISKGLKIPVVIITGDDSADLLTEASSLGVA
                     KVIQKPVQPDRLLQTVQRILKRQGLNPAPLAVEGRATRYSAEELMDQTIELAADNARR
                     GRGGPYAALVATKEGQILGTGANGRSARVDPTAHAEVMAIRKAVEKTGRDDLSDCILY
                     CSSQPTKVGEALIVSVGIQQIYYGLSHEDTGQIKVHKQASQPTYSQLCQNQALEMFKT
                     AKKD"
     gene            1520..2176
                     /locus_tag="PK513_07770"
     CDS             1520..2176
                     /locus_tag="PK513_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82383.1"
                     /translation="MIYKTNRKKEAGNVLFLILVGVMLFGALSFTVAQMLRTGDAEGV
                     GERKASVLADEILATAHQYRQAVQALRISNSCEDEDISFENSTVGGYTHTPVASDNCK
                     LFHTDGGGLSYIAPSTEYGDGSDWVFNGSNIVDNIGTTAPDLIAILPNINLVVCNAIN
                     DKMGITALGNDATIDFSKFTDTYSSTQTIDFAAGFAAGCLDYVNSGDNYFFYQVLIAR
                     "
     gene            complement(2188..4503)
                     /gene="clpA"
                     /locus_tag="PK513_07775"
     CDS             complement(2188..4503)
                     /gene="clpA"
                     /locus_tag="PK513_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOO82384.1"
                     /translation="MLSKNLEQTLHKALAIAGQYHHEYATLEHLLHALTDDQDAMAVL
                     RSCGISIPDLRDQLTAYIENELTYLINMDAEESKPTTAFQRVLQRAAIHVQSSGREEV
                     TGANVLVALFSERESYAVFFLQERDMTRFDAVNYISHGIAKVPTQGDTSRTPRGTDED
                     SIKEVKTGDEALDAYCVNLNEKAKDGKIDPLIGRDKEVERTIQVLCRRSKNNPLYVGD
                     PGVGKTAIAEGLAKKIIEKDVPEVLHDATIFSLDMGALLAGTRYRGDFEERLKMVIKE
                     LEQIDGAVLFIDEIHTIIGAGATSGGAMDASNLLKPALSNGSLKCIGSTTYKEYRNHF
                     EKDRALVRRFQKIDVYEPSVEDAIAILMGLKPYYEKHHGVEYTDEAIEAAVKLSDKYM
                     GDRKLPDKAIDIIDEVGAAQMLVPEDKRKKLIKQADIEGVVAAIARIPAAAMTQSDRE
                     SLQNLERDLKTMVYDQDKAIDILVDSIKMARAGLREDEKPIGSYLFSGPTGVGKTEVA
                     RQLSKTMGIELIRFDMSEYMERHAVSRLIGTPPGYVGFEQGGLLTDKIDQHPHCVLLL
                     DEIEKAHPDLFNILLQVMDYGKLTDNNGKTIDFRNVILIMTTNAGAADIARTPIGFGR
                     EIGDSGDDYADNEAIIKTFAPEFRNRLDAIVPFKNLSHDTVEKVVDKFIAALEGQLAD
                     KNIEIILTDKARKYLAKEGYDPAMGARPLSRVIQEQVKRPLAEEILFGKLEKGGQVKV
                     DLKGDELTFAYKTEKSKTSENTEDETEEPAE"
     gene            complement(4518..5561)
                     /locus_tag="PK513_07780"
     CDS             complement(4518..5561)
                     /locus_tag="PK513_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO82385.1"
                     /translation="MHDGEGMRFLHIKIILVPFALLFITAPAKAECLDKDINYEGTIE
                     VFSAMAMAHDSFLSSRTDIEKRAQDGDEKAQGILNFLNEEINLDAARAGDNFHRMWIA
                     FRRAYGELLTDDLNNHKMQIDDKEIFKWVLQAAEKEDYRAYHLLDWYYTQGIGTEKNI
                     QEAFRWRLKLAEEAECYYKIDNALGPLEGNLAENFLHGTGGAEKNTKEGIKWYESAAS
                     HNHKVAHLMLGFLYQLSGETKMEGKPVTDPYFTKSDVPKNYKKAIEHFTAYMTLDPEA
                     KELHYMIALMYEAGGYGIEKDLAKAFKHMKISAENGKSDAQKALAQYYEQGIGIEKNA
                     KEAKKWHKLAKEN"
     gene            complement(5540..5887)
                     /gene="clpS"
                     /locus_tag="PK513_07785"
     CDS             complement(5540..5887)
                     /gene="clpS"
                     /locus_tag="PK513_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOO82386.1"
                     /translation="MSDEDPAYSDSGDDTQESDETGLMLKTRPKTKKPAMYKVLLLND
                     DFTPMEFVVHVVEKFFAKNRAEATEIMLHVHRRGVGICGVFTYEIAETKVAQVMDFAR
                     ANEQPLQCTMEKE"
     gene            complement(6068..6445)
                     /locus_tag="PK513_07790"
     CDS             complement(6068..6445)
                     /locus_tag="PK513_07790"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOO82387.1"
                     /translation="MTKQPFQFDKMAQDAANQSREGMEAFVKSGTIFAKGFEEIIKTA
                     ASMSQNVAEKQAEFARQLMSSKTLNEYAEAQNKIAQANFDQFMSGATQISEMSVKVLN
                     EASEPLNAQMTKAMAKANKQMAA"
     gene            6444..6731
                     /locus_tag="PK513_07795"
     CDS             6444..6731
                     /locus_tag="PK513_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82388.1"
                     /translation="MIFSISLKGSNAPVIAGGDIGILGRLVTTFVATFFTAGLAADFA
                     AVFFTLALAVFFTVVFFATVFFADLFDTVFSAGDFFTAITIYPSLFYASAV"
     gene            7096..>7614
                     /locus_tag="PK513_07800"
     CDS             7096..>7614
                     /locus_tag="PK513_07800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOO82389.1"
                     /translation="MHTSAFAKGNPKYASIVMDADTGLILHQRYANKSLHPASLTKVM
                     TLLLTFEALEQGRISLNDRVRISNHAANMVPSKLGLSPGSSIRVKDAIYALVTKSAND
                     VAVALAEHLAGSERNFAQRMTLKAHDIGMTRTTFVNASGLHDERQVSTARDMAKMARY
                     VIQVYPEYYRYFS"
BASE COUNT         1773 a         1749 c         1870 g         2222 t
ORIGIN      
        1 agaagttgat atgcgcgggc gataaaacgc ctagaaagct taatagtccc ataatatgcc
       61 tataatccgc ttgtgttttt ctttacaaaa gctcgataga atttaatatc acgaaaaagt
      121 gaaaaggaca ccccccatat gattttacaa gagaagttac aaacacttgg ccttgaactg
      181 ccgcaagcag cggcaccggc ggccaactat gtcccgtacg tggttgcgga tgacgatatg
      241 ctgtatatag ccgggcagat tccgttttta aatggtgagc agatgcataa agggcggttg
      301 ggcgaagatt taagcgttga gcaaggcgtg gaagcagctc aggcttgcgc gctgaatatt
      361 ctggcccagg ccaatgcggc tgtagatggc gattggggta aggtcaaacg cctggtcaag
      421 ctgggcgggt ttgtgaattg tacgcctaat ttttacgatc atccgaaagt tattaacggt
      481 gcatctgacc tgatggttaa agtgatgggg gaagcaggca aacatgcgcg ttttgctgta
      541 ggcgcgccat ctttgccgct gggcgtgtcg gttgagattg atgcgatttt cgagttgttt
      601 aggttctagt cttttttcgc agttttaaac atttccagcg cctgattttg gcaaagctgg
      661 ctgtaggtgg gctgagaagc ctgtttgtgg accttgatct ggccggtatc ttcatgggac
      721 aggccgtaat agatctgctg aatgccaacg ctgacgatca gcgcttctcc gactttggtc
      781 ggttgggatg agcaatatag aatacagtcc gataaatcat cacgcccagt tttttcaact
      841 gccttgcgga tggccattac ctcggcatgg gcggtcggat caacacgcgc ggagcggcca
      901 ttagcaccgg tgcctaaaat ctggccttct tttgtcgcaa ccaatgccgc atacggcccg
      961 ccgcgccctc ggcgtgcgtt gtctgcggca agttcaattg tctggtccat cagctcttca
     1021 gcgctgtagc gcgttgcgcg cccttcaacg gctaatggcg cgggattgag cccctgacgc
     1081 tttaagatgc gctggaccgt ttgaagcaaa cgatccggtt gcacgggttt ttggatgacc
     1141 ttggccacgc ccaaagagct ggcctccgtt aaaagatccg cagaatcatc acctgtaata
     1201 atcacaaccg gaattttcag ccccttggat atcacatatt tcatgaattc aaaaccgccg
     1261 gtaggtgcca tattgatgtc aaccagcgct atatcaacca gattgtcgtc aaccactcta
     1321 atcgcgcttt cgccatccac agcgcccaga acattatagc cctgcgcacg caaaataccg
     1381 gtcatcatat cgcggctgac ctcgttatct tcggcgataa gaacatttat aaagctgtct
     1441 tcactcatga gcgtattaaa gcatattttg aacaaatggg ggggagaaaa atatcctttg
     1501 aggtgataca atgtttgtca tgatttataa gacaaataga aaaaaagaag cgggcaacgt
     1561 gctgtttctg attttggtcg gggttatgct gtttggcgcg ctgagcttta cagtcgcaca
     1621 gatgctacgt acaggtgatg cggagggcgt aggagagcgc aaagccagcg ttttggctga
     1681 cgagatttta gcaactgcac atcagtaccg gcaggccgtg caagcgctgc gcatttcaaa
     1741 tagctgtgaa gacgaagaca tcagttttga aaactctacg gttggtggat atacgcatac
     1801 accggtggca agcgataact gtaaattatt tcacactgat ggtggcggac tgtcttatat
     1861 cgccccgtca acagaatatg gtgacggcag tgattgggtg tttaatggct ctaatattgt
     1921 tgataatatc ggcacaaccg cgccggattt gattgctatt ttgccgaata tcaatctggt
     1981 cgtatgcaat gccattaatg ataaaatggg catcactgcg ctgggcaacg atgcaacgat
     2041 cgatttttcg aagtttaccg atacatattc ttctactcaa actattgatt ttgcagccgg
     2101 ttttgcagca ggctgtttgg actacgtcaa tagcggtgat aattactttt tctatcaggt
     2161 gctcatcgcg cggtaatgat gcggtgatta ttcggcaggc tcttctgtct catcctcggt
     2221 gttttcagac gtttttgact tttcagtttt ataggcaaag gtcagttcgt cgcctttaag
     2281 atcaacctta acctgaccgc ctttttccag cttcccaaac aggatttctt cggccagagg
     2341 ccgtttgacc tgttcctgga tgacgcggct taagggccgc gcgcccatcg cggggtcata
     2401 accttctttg gccagatatt tgcgtgcttt atcagtgaga ataatttcga tatttttatc
     2461 ggcgagctgg ccttccagcg cagcaatgaa tttatcgacg accttctcaa ccgtatcatg
     2521 agagaggttt ttgaacggta caatcgcatc aaggcggtta cgaaattccg gagcgaaagt
     2581 tttgatgatt gcttcgttat cggcgtaatc atcgccggaa tcaccaattt cacggccaaa
     2641 accgatcggc gttctggcaa tatcagcggc gcccgcattt gtggtcatga taaggataac
     2701 attgcggaaa tcaatggttt tgccgttatt gtcggtcagc ttgccataat ccatgacctg
     2761 cagcaaaatg ttgaacaggt cggggtgggc cttttcaatt tcatccaaaa gaagaacgca
     2821 gtgcgggtgc tggtcgattt tatcggtgag caggccgcct tgttcaaagc cgacatagcc
     2881 cggcggtgtg ccgatgaggc gggaaacggc gtggcgctcc atatattcgg acatgtcaaa
     2941 gcggatcaat tctatgccca tggttttgga aagctggcgc gcaacctcgg ttttccccac
     3001 gccggttggg ccggaaaaca gatatgagcc gatgggtttt tcatcttcgc gcaggcccgc
     3061 gcgcgccatt ttgatggaat cgaccagaat gtcgatggct ttgtcctgat catagaccat
     3121 ggttttaagg tcgcgctcga gattttgcag agattcgcgg tcgctttgtg tcatggcggc
     3181 tgccgggatg cgggcgatgg ctgcaacaac gccttctata tcggcctgtt tgatcagctt
     3241 tttgcgcttg tcctcaggaa caagcatctg cgccgcgccg acctcgtcga taatatcaat
     3301 ggctttatcg ggcaatttgc gatcgcccat atatttgtcg gaaagcttta ccgccgcttc
     3361 aatcgcttca tccgtatatt ccacgccgtg gtgcttttcg taatagggtt tcaagcccat
     3421 cagaatcgca atcgcatctt cgacggaggg ttcataaacg tcgattttct gaaagcggcg
     3481 cacgagcgcg cggtcttttt cgaaatggtt gcggtattcc ttataggtgg tcgagccgat
     3541 gcatttgagg ctgccgttgg acagggcggg tttaaggagg ttggaagcat ccatcgcccc
     3601 gccggaggtc gcgccggcgc cgataatggt gtggatttca tcaataaaca ggaccgcacc
     3661 gtcgatttgc tcaagttctt tgataaccat tttcaggcgt tcttcaaagt cgccgcggta
     3721 gcgggtgcca gcgagcaacg cgcccatatc aagagagaaa atcgtggcat cgtgcagaac
     3781 ttcaggtacg tctttttcaa tgatcttttt ggcgaggcct tcggcgatgg cggttttgcc
     3841 aacgcccgga tcgccaacat aaagcggatt gtttttagaa cgccggcaca gaacctggat
     3901 cgtgcgctcc acttctttat cgcggccaat caaaggatca attttgccgt ccttcgcctt
     3961 ttcattgagg ttcacgcaat aggcatcgag ggcttcatcc ccggttttga cctccttgat
     4021 gctgtcttcg tcggtgccgc gcggtgtacg ggatgtatcg ccctgcgtcg ggactttggc
     4081 aatgccgtgg gagatgtagt tgaccgcatc aaagcgcgtc atatcgcgct cttgcaggaa
     4141 gaaaacggcg taactttcgc gctcggaaaa cagggcgacg agcacgttgg cgccggtgac
     4201 ttcttcgcgg ccagaggact ggacatgaat agcggcgcgc tggagaacgc gctggaaggc
     4261 cgtggtgggt ttggattctt cagcgtccat attgatgaga taggtcagct cgttttcgat
     4321 ataggcggtg agctgatcgc gcagatcggg gatcgaaatg ccgcaggagc gcagcacggc
     4381 catcgcgtcc tgatcgtccg tcagcgcgtg aaggaggtgt tccagcgtgg cgtattcatg
     4441 atggtactgc ccggcgatgg cgagggcttt atggagggtt tgttcgagat tttttgagag
     4501 catttatttc gctttttcta attttctttt gccagtttat gccatttttt agcttctttg
     4561 gcattttttt ctatgcctat gccttgctcg taatactgtg caagtgcttt ttgagcatca
     4621 gacttcccat tttcggcaga aattttcata tgcttaaatg cctttgccaa atccttttca
     4681 ataccataac cgccagcttc atacattaga gcgatcatgt aatgtaattc ctttgcttcg
     4741 ggatcaagcg tcatataggc tgtgaaatgt tcgatcgctt ttttataatt ttttggaaca
     4801 tcacttttag tgaaatatgg gtctgtgact ggttttcctt ccattttggt ttcaccactc
     4861 agttggtaca agaaacccag catcaaatga gcaactttgt gattatggct tgcagcactt
     4921 tcataccatt ttatgccttc tttagtattt ttctcggctc ctcctgtccc atgcaaaaaa
     4981 ttttccgcta aatttccttc aagaggacct aacgcattgt ctatcttata ataacattca
     5041 gcctcttcag ccagcttgag ccgccatctg aaagcttcct gaatattttt ttctgttccg
     5101 attccttgtg tgtaatacca gtctaataag tggtaagctc tataatcttc tttctcagcg
     5161 gcttgtaata cccatttgaa aatttcttta tcgtcaatct gcattttgtg attgtttaaa
     5221 tcatctgtca gcaattcacc ataagcacgc cgaaaggcaa tccacattct atgaaagtta
     5281 tcacccgcac gcgcggcatc cagatttatt tcctcgttaa gaaaattaag gatcccttgt
     5341 gctttttcat caccatcttg cgctcttttt tcgatatctg ttcttgagga taaaaaacta
     5401 tcatgcgcca tagccatggc actaaaaact tctatcgttc cttcatagtt tatgtcttta
     5461 tcgagacatt ctgcttttgc aggagcagta ataaaaagaa gagcaaatgg aacgagaata
     5521 atttttatat gtaagaatct cattccttct ccatcgtgca ttggaggggt tgttcgttgg
     5581 cgcgggcgaa atccatcact tgcgcgactt tggtttcggc gatttcgtag gtgaagacgc
     5641 cgcagatgcc gacgccgcgg cggtgaacat ggagcatgat ttcggtggct tcggcgcggt
     5701 ttttggcgaa gaatttctca acgacatgga cgacgaattc catcggcgtg aaatcatcgt
     5761 tcaaaagcaa gactttgtac atggcgggtt ttttagtctt gggccgggtt ttgagcatca
     5821 ggccggtttc atcggattct tgcgtatcat cgccggaatc gctgtaggcc ggatcttcgt
     5881 cactcatgtg tggaaatatc tctttatcgc caatcatcat atttttagcc agaactcact
     5941 tcatatcgtt tatggatacg ctattatttt atcataaaag gcccgcttga gaatagatat
     6001 gtgtgttttt gggtaaatta aaagccccgc aagaatctgc agggcttttt aaaagctttt
     6061 aaagcgctta agccgccatt tgtttattgg ctttagccat cgcttttgtc atttgtgcat
     6121 tcaaaggctc ggacgcttcg ttcaaaacct tgacgctcat ctctgaaatt tgtgttgcgc
     6181 cagacatgaa ctgatcgaag ttggcctggg caattttatt ttgcgcttca gcatattcgt
     6241 tgagcgtttt tgagctcatc aattgtctgg caaactccgc ttgtttttct gcaacgtttt
     6301 gagacattga agctgcagtt ttgataattt cttcaaaacc tttcgcaaaa atcgttcccg
     6361 attttacaaa agcttccatg ccctcacggc tttgattagc ggcgtcttgc gccattttgt
     6421 caaattggaa aggttgtttt gtcatgatct tctccatttc tttaaaaggg tccaatgcgc
     6481 ccgtaatagc aggcggggac atcggcattt taggaaggct tgttacaact tttgtagcca
     6541 ctttttttac agcaggctta gcagcagatt ttgctgcagt tttctttacg ctggctttgg
     6601 ctgttttctt cacagttgtt ttttttgcca cagttttttt cgccgatctt ttcgacacgg
     6661 tcttttccgc aggtgatttc ttcacagcca taacaattta tccttctttg ttttatgcaa
     6721 gcgcagtatg acagcaaata caaaatttgc aagtctgatt gtgcatcaca acacgaaaat
     6781 tttttagcat atatttttta tatccgtcaa cattttttat tgcagcgcac aaaaatagat
     6841 tatcggcgct ggtaaagaag tttcccacat gttgcattag acaaggaaca caaaaaactg
     6901 tatcatcagc actggacgtt ttactctttt caggtttagg catagggctt gaaaacctct
     6961 tcatttgaac gcaggcacga cgtgacattt aacagagcat atacaggctg taatcatttt
     7021 gacggttttt cggcatcggt taggcgcgcc gcttttttat ttacgcttgt catacttttc
     7081 ctggggattg caagtgtgca tacaagcgca tttgccaagg gcaatccaaa atatgcttcg
     7141 atcgtgatgg acgcagatac ggggctgatt ttgcatcagc gctacgcgaa taaatctttg
     7201 catccggcat cactgacaaa agtcatgacg ttgctgttga ccttcgaagc gttagaacaa
     7261 ggacgtatca gtttaaacga ccgggtacgg atttcaaacc acgccgcaaa tatggtgccc
     7321 agtaagttgg ggctgtctcc cggttcgagc atccgggtta aagacgcaat ttacgcgctg
     7381 gtaacaaaat cggccaacga tgtggcggtg gctttggccg agcatctggc aggttcagag
     7441 cgcaattttg cccagcgcat gacgttaaag gcgcatgaca tcggcatgac acgcacgact
     7501 tttgtaaacg cttcgggtct acatgatgag cgccaggtca gtacagcgcg tgacatggcg
     7561 aaaatggcac gctacgtcat tcaggtttat ccggagtatt accgctattt ttcc
//
LOCUS       DAOSWR010000102         7469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6401_length_7469_cov_6.005665, whole genome shotgun sequence.
ACCESSION   DAOSWR010000102 DAOSWR010000000
VERSION     DAOSWR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_7469_cov_6.005665"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..417)
                     /locus_tag="PK513_07805"
     CDS             complement(1..417)
                     /locus_tag="PK513_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOO82390.1"
                     /translation="MAASKILAACLMLASQTYSVPPAVLVGIYKAEGGKVGQEVVNSN
                     GSYDLGPMQINTIWLPDLAKRWGVNENTARKWVRDDACTNVGVAAWIFKGHLDETGSL
                     SKAIAHYHSRTPHHGSRYKSRVIDIMKDNGLIKTGQ"
     gene            complement(458..1696)
                     /locus_tag="PK513_07810"
     CDS             complement(458..1696)
                     /locus_tag="PK513_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82391.1"
                     /translation="MDQNAAYLLQMLETLGAPLMSAIVSAQPLDQSTTLQSEAQSIAA
                     LLGKTVETSIDLGQIMDINPAEAQDDTLRVALAGLAGPLVAGQYVRRGQVPETADLKK
                     ITNALQAVLSFSDNFTPTAETIGRLKNLQASGQIVDSYQTQIQYMQAFIPVIDAIAAF
                     PFGQPEAKLIMDVSARLVKKAVDLRETLLPGIDDEAVQKRTELGLLNAVATLYSNCHK
                     AETDKVLNTEDTSREGGLSIEPVWTAFETRAAMLEALAARIAPGGVKKSDDSSSKAPV
                     SVLPTIAPQEPVSPPPAETQQPQEPVIPKTPVAAAPSIQPPAEIQAQGNPPGQNPMAM
                     FAKSKTDAAAPSEPPQAPPIQSPPQVPPASGEKPPVPAAPPPPPEQPSPAAPPVLPSD
                     QSGENSGSGPMSFFTKKGEE"
     gene            complement(1824..2669)
                     /locus_tag="PK513_07815"
     CDS             complement(1824..2669)
                     /locus_tag="PK513_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOO82392.1"
                     /translation="MTRFFVFLTLVLFIGAVPALAQPTEPLPQLPDPIQNLVNEGAQI
                     RFLGKDHGLDAWLTIKNGVEQYFYVLPDGEAFVMGVLFDKTGKLVTVNQVSRLRSEGD
                     TLLDTLAEFPIPEASDTEKPFEFQTPAEQMFADIESSNWVPFGRLDAPIIYSFIDTQC
                     PHCHAFMQELINGEYFDSGKVQLRMIPVGFKEQTQAQAAFLIASPNPQERWFKHMKGD
                     ETALPAKTEINQQGVQRNLAIMQSWKFDATPMVVYRGRDGSVKIIRGKPQDLKAVLAD
                     LKTSN"
     gene            complement(2723..3958)
                     /locus_tag="PK513_07820"
     CDS             complement(2723..3958)
                     /locus_tag="PK513_07820"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOO82393.1"
                     /translation="MTLISSENFISCASGGDDWREAAKSVLEQLSDAQLSKNGFNFGF
                     LFVSDALADDAESILNLFKSVLKIENWVGGIGLGVCAGGQSFIDGPAVSVMLGKFDPE
                     NFCIFPPINLEPEKAKAVMVPWLEAHDPMLVFVCGDPMSEEDPALLLHNLDMACNGFM
                     VGGLTSSRNSHAQFAVDFYQGGLCGALFSDNVPVASMLSQGCDPIGGVHTITRCDGHE
                     ILELDGQKAVEVFEHDLRAMAIKKLDVDPDQVLIDEEVLDNPDSMPEEFQSLIQGEVH
                     AALAISESDGQDYLVRNIIGINPDDGAMMISQHVFNGERLMFVHRDHESVYRDLSASL
                     IDLRKRVQEEYGEFAPKGALYISCAARAYNEFDDQQKNEITLIQEIIGDVPLTGFYAG
                     GEINKGRLYGYTGILTLFL"
     gene            4052..4618
                     /locus_tag="PK513_07825"
     CDS             4052..4618
                     /locus_tag="PK513_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO82394.1"
                     /translation="MEALWIILGLVVLTGLVVITIYNRLVALRQNREQSFADIDVQLK
                     QRFDLIPNLVKTVKGYAAHEKEVLENVTNARAGVASAGNMSQRVSAENALSRAMISLF
                     AVAENYPDLKADGTFNKLMDELSDIENKLAAARRFFNNATAELNTAVEQFPAVLFAGM
                     FGFSKEEFFEVPEDARETLEKAPDVSFE"
     gene            4625..5740
                     /locus_tag="PK513_07830"
     CDS             4625..5740
                     /locus_tag="PK513_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO82395.1"
                     /translation="MPIAVAGLQTHIWNNNAKSMMLLALYPLLIIGMIWLLSFASGYI
                     YYGGNIHSYDGIMNGQTPWGHQDTTLQSPTAAVNLANYIVINNLWLIFTVIFGWFAIS
                     WFFHTKMIRKLAHSRPVTRKEEPALYNLLENLSITAGITMPKLEIIETHARNAFASGI
                     DEKSYTITVTRGLLNMLSKNELEGVLAHELTHIINRDVRLLIVTVIFAGMIGFFAQLV
                     WSNLRYSFIFSRHERRSGKGLLIMIVLVAVLWVGYLLTLSTRFALSRRREYMADAGAV
                     QLTKKPESMMRALLRIAQRDQIPETTDDIMMMCIENSNPFMGLFATHPPIEKRIQILS
                     EMTNTPVPQITTLSAEKEKRIEHVKKNPWLTRGRRKP"
     gene            5852..6568
                     /locus_tag="PK513_07835"
     CDS             5852..6568
                     /locus_tag="PK513_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOO82396.1"
                     /translation="MQTDRFQTTQTRTREIDEGLRQHMNNIYAKMTAGVLVTAIVAMV
                     VSSSPFLYKLFMVGPQQTIVMLAPLAIVWFGFRPDRMSSGKLMMVFFLLAVLYGISFS
                     TIAVVMAASPEFGVTVAKAFFIATGMFAGMSVFGYTTKRDLSPMITFLVMGIWGVVIA
                     GLLNYFIFHSPMLSMLRSMIAIPLFAGVTAWQTQNMKEMYSPSMPQEFANRMGWAAAL
                     TLYISFIAMFANILNLMSQR"
     gene            6688..6906
                     /locus_tag="PK513_07840"
     CDS             6688..6906
                     /locus_tag="PK513_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO82397.1"
                     /translation="MQTMNEGLQRAAGILPQTYDMRGLKTLAAFERDYIEHILQICQG
                     NIPRAAEILDIAPSTLYRKRAAWDKNRV"
     gene            7009..7425
                     /gene="mscL"
                     /locus_tag="PK513_07845"
     CDS             7009..7425
                     /gene="mscL"
                     /locus_tag="PK513_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018148576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOO82398.1"
                     /translation="MLKEFKEFIARGSVMDMAVGIIIGAAFTAIVNSMVNDMIMPVIG
                     VITAGIDFGDLFIALDGNSYATLAAAKEAGAAIVKYGAFINAVINFLIVAFVIFILVK
                     GVNKLKRAEEEAPAKVPKPPKSEVLLEEIRDALVKK"
BASE COUNT         1971 a         1893 c         1706 g         1899 t
ORIGIN      
        1 ttactgtccg gttttgatga gcccattatc tttcataatg tcaatcacac ggcttttgta
       61 acgactaccg tgatgcggcg tacgcgaatg gtagtgggcg atagccttgg acaaactgcc
      121 ggtttcatcc agatgccctt taaaaatcca tgccgcaaca cccacattag tgcaagcgtc
      181 atcacgcacc catttgcgcg cagtgttttc attgacgccc cagcgtttgg ccagatcggg
      241 aagccagatc gtattaatct gcatggggcc aagatcataa gagccgttac tgttaacaac
      301 ctcctgaccg actttaccgc cctcagcttt gtaaataccg accagaaccg caggtggaac
      361 ggaataggtt tgagaagcga gcattaaaca tgcggcaaga attttactgg cagccataat
      421 tcatcatgtc ccgaatatga gcgcggaaaa aaccatctta ttcctcgcct tttttagtga
      481 aaaaactcat cggacctgat ccgctattct caccactctg atcactaggc agcaccggcg
      541 gcgcggccgg tgaaggttgc tctggaggag ggggaggggc tgcaggaaca ggcggtttct
      601 caccggaagc cgggggtact tgcggcggcg attgtatggg gggagcttgt ggcggctctg
      661 agggcgctgc agcatcggtt ttggattttg caaacatggc catgggattt tgtccaggtg
      721 gattgccttg cgcttggatt tcggcaggtg gctgtataga cggcgctgca gcaacagggg
      781 ttttgggaat gacaggctct tgtggttgct gggtttcggc aggcggcggg gaaacaggtt
      841 cttgcggcgc tattgttggc agaacactaa caggggcttt gctggaagaa tcgtcacttt
      901 tctttacacc tccaggcgca atgcgcgcag ctaaagcctc caacatcgca gcacgcgttt
      961 caaacgcagt ccataccggc tctatactga gaccgccttc gcgcgaagta tcttctgtgt
     1021 ttaaaacttt atccgtctca gctttgtggc aattgctgta aagagtggcc accgcattaa
     1081 gcaatccgag ctctgtgcgt ttttgcacgg cctcatcatc aataccgggc aaaagtgttt
     1141 cacgcagatc tacagctttt ttcaccagtc gtgcactcac atccataatc aacttggctt
     1201 caggctggcc aaatggaaaa gcagcaatgg catcaatcac gggaataaaa gcctgcatat
     1261 attgaatttg cgtttggtaa gaatcaacga tttgtccgga agcttgaagg tttttaaggc
     1321 gtccaatcgt ttcagctgta ggcgtaaaat tatccgaaaa acttaaaaca gcttgcagag
     1381 cattggtaat tttttttaga tctgcagttt ctggcacctg cccacggcgc acatattgtc
     1441 cggcgaccaa gggcccggcg agcccggcca aggcaacgcg caatgtatca tcctgtgcct
     1501 cggccggatt aatatccatg atctggccaa gatcaatgct tgtttcaact gttttcccca
     1561 aaagcgcggc gattgattgc gcttcagatt gaagagtggt tgactggtct aagggctgtg
     1621 cggacacaat tgcactcatg agaggagcgc cgagtgtttc gagcatttga agaagataag
     1681 cggcgttctg atccatattt ataaagtttg ttaagaggcc tggtgaatga aaaggaagtg
     1741 tttaaatata cctcaacact tcacagtata aggtgcttta cccacctgcg gcaagacccc
     1801 aacatcaaag cacatcacta catttaattt gatgttttca gatcggctaa aacagctttt
     1861 aggtcctgag gtttcccgcg gataattttt actgaaccat cgcgcccacg gtaaacaacc
     1921 attggcgtag catcaaattt ccatgattgc atgatggcaa ggttgcgttg caccccttgt
     1981 tgattaatct cggttttggc gggcaaggcc gtttcatcgc ccttcatatg tttaaaccag
     2041 cgctcttgcg ggttaggcga agcaatcaaa aatgctgcct gcgcctgcgt ttgctcttta
     2101 aatccgactg gaatcatccg caactgaacc ttaccgctat caaaatactc gccatttatg
     2161 agctcttgca taaaggcatg gcaatgcggg cactgcgtat caataaaact ataaattatt
     2221 ggcgcatcaa gccgcccaaa aggcacccag ttgctgctct caatatcggc aaacatctgc
     2281 tctgccgggg tctgaaattc aaaaggtttt tcagtatcgc tggcttctgg aattggaaat
     2341 tctgccagcg tatcaagcaa cgtatcgcct tcggagcgca aacggctgac ctgattgacc
     2401 gtcaccagtt taccggtttt gtcgaacaaa acacccataa caaaggcttc accatcgggc
     2461 agcacataaa aatactgctc cacaccgttt ttaattgtca gccaggcatc aagcccatga
     2521 tcttttccta aaaagcgaat ctgcgcgcct tcattcacaa gattttgaat gggatcgggc
     2581 aactggggca ggggctctgt cggttgagcc agcgcaggca ctgctccaat aaataaaacg
     2641 agtgtcaaaa agacgaaaaa acgagtcatg aaactatcct gtttgaaaaa caacaccaag
     2701 agttaagtct aacgcatttt ccctacaaaa acagcgttaa aatgccggta tagccgtaaa
     2761 gccgtccctt attaatttct ccaccggcgt aaaatcccgt caacgggaca tcgccaatga
     2821 tttcttgaat cagcgtaatt tcattttttt gttgatcgtc aaattcattg taggctcgtg
     2881 cagcgcagga aatatagagc gcacctttag gcgcaaattc cccatactct tcctgtacac
     2941 gctttcgtaa atcaatcagg ctggcactta agtccctata aacgctttca tggtcgcgat
     3001 gaacaaacat taagcgctca ccattaaaaa catgctgcga gatcatcatc gcgccatcat
     3061 cgggattaat ccctataata ttgcgcacca gataatcctg cccgtcggat tcggaaatcg
     3121 ccagagctgc atgaacctca ccttgaataa gactttgaaa ctcttcgggc atagaatccg
     3181 gattatccaa aacctcctca tcaatcagca cttgatctgg atctacatcc agttttttaa
     3241 tcgccatcgc gcgcaaatca tgctcaaaaa cctctacagc cttttgtcca tccagttcaa
     3301 gaatttcatg cccgtcgcac cgggtaatcg tatgaacgcc gccaatagga tcacaacctt
     3361 gcgatagcat gctggccacc ggtacgttat cggaaaacag cgcgccgcac aggccgcctt
     3421 gataaaaatc cacggcaaat tgcgcatggc tgttacgtga agaggtaagg ccgccgacca
     3481 taaagccgtt gcaggccata tccagattgt gcaaaagcaa cgccggatct tcttcgctca
     3541 tcggatcgcc gcaaacgaag accagcatcg gatcgtgcgc ttccagccac ggaaccataa
     3601 cggccttggc cttttcaggt tcaagattga ttggcggaaa aatgcaaaaa ttttccggat
     3661 caaactttcc cagcatcacc gaaaccgctg gcccgtctat gaagctttgc ccgcccgcgc
     3721 acacgcccag accaatgcca ccaacccagt tctcaatctt cagaaccgac ttaaacagat
     3781 ttaaaatact ttccgcatca tctgccagag catcggatac aaataaaaac ccgaaattaa
     3841 atccgttttt agacagctgg gcatcgctca attgttcaag cacacttttt gcggcttcgc
     3901 gccaatcatc gccaccggat gcgcaggaaa taaaattttc tgaagagatg agggtcataa
     3961 cggcaagctt ctcgacttcg gattccaata atgcttaact atataacctg agcgcaataa
     4021 gaaaagggct ttgcccatta aggaggagtt tatggaagct ttatggatta ttttagggct
     4081 ggtggttttg acgggtttgg tcgtcataac catttacaat cgtctggttg ctctacgcca
     4141 aaatcgcgag caaagctttg cggatattga tgtacagctc aaacagcgct tcgatctcat
     4201 tcccaatctg gttaaaactg tgaaaggcta cgccgcgcat gaaaaagaag tgttggaaaa
     4261 tgtcaccaac gctcgcgctg gcgttgcaag tgcaggcaac atgtcacagc gcgtgagcgc
     4321 ggaaaacgct ttgagccggg ccatgatttc actttttgcg gttgccgaaa actaccccga
     4381 tctgaaagcc gacggcacct ttaacaagct gatggacgaa ctctccgata ttgaaaacaa
     4441 actggctgca gcccggcgct tctttaacaa cgccacagcc gaactgaaca cggccgttga
     4501 acaattcccg gctgttttat ttgcaggcat gtttggcttt agcaaggaag agtttttcga
     4561 agtccccgaa gacgcgcgtg aaacgctgga aaaagccccg gatgtaagtt tcgagtagac
     4621 gagcatgcca atcgctgtag ccggacttca aacccatatc tggaataaca acgccaaatc
     4681 aatgatgctc ctggcgcttt atccgctttt gattatcggg atgatctggc tgctctcttt
     4741 tgccagcggt tatatttatt atggcggtaa tatccattca tacgacggca ttatgaacgg
     4801 ccagacacct tggggtcatc aggacacaac tttgcaaagt ccaacagccg ctgtaaatct
     4861 tgccaattat atcgtcatta ataatctctg gctgattttt acggttattt tcggctggtt
     4921 tgccatttcc tggttttttc acacaaaaat gattcgaaag ctcgctcatt cccgcccagt
     4981 cacgcgcaaa gaagaaccag ccctttataa cctgctcgaa aatctctcaa tcacggccgg
     5041 cattaccatg ccgaaactgg aaatcatcga aacgcatgcc cgtaatgctt ttgccagtgg
     5101 catcgatgag aaatcctata caattaccgt tacaaggggc cttttaaaca tgctcagcaa
     5161 gaatgaactt gaaggtgtcc ttgcccatga actcacccac atcatcaacc gcgatgtacg
     5221 cctccttatc gtcaccgtca tctttgccgg aatgatcggt tttttcgccc agctcgtatg
     5281 gagcaatttg cgctacagtt tcatcttttc aagacacgag cgccgtagcg gcaaagggct
     5341 tcttatcatg atcgtccttg tggccgtcct ttgggtcggt tatctcctga ccctgtccac
     5401 gcgctttgcg ctttctcgcc gccgcgaata tatggccgac gccggagcgg tgcagctcac
     5461 caaaaaaccg gaaagcatga tgcgtgccct cctgcgcatc gcccagcgcg atcagatccc
     5521 ggaaaccacg gacgacatca tgatgatgtg cattgaaaat tcaaacccct ttatggggct
     5581 tttcgccacg catccgccaa tcgaaaaacg gatccaaatt ctttccgaaa tgaccaatac
     5641 gccggtgccg caaattacaa cgctaagcgc cgaaaaagaa aaacgcattg aacatgttaa
     5701 aaaaaatcca tggctcacgc gcggacgtcg aaaaccgtaa catactctac aaacagtgtt
     5761 ttccaatttt cccctgaaaa tctttgaaat ctgctacaat aaagactata tgtattgaaa
     5821 atcgtttgaa tatcataagg agattgtaac gatgcaaact gaccgtttcc aaaccacgca
     5881 aacccgcacc cgcgagattg atgaaggttt gcggcaacat atgaataaca tttacgccaa
     5941 aatgaccgcc ggtgttttgg taaccgccat tgtggctatg gtggtgagta gctcaccgtt
     6001 tttatacaag ctattcatgg tagggccgca acaaacgatc gttatgcttg cgccgttggc
     6061 aatcgtctgg tttggtttta gacccgaccg catgtcttct ggcaagctga tgatggtgtt
     6121 tttcctgctg gccgtgcttt acgggatcag cttttccacc atcgctgtgg ttatggcggc
     6181 cagccccgaa ttcggcgtta ccgtagctaa ggctttcttc atagccaccg gtatgttcgc
     6241 cggaatgagc gtgtttggct ataccacgaa aagagatctc tcgcccatga tcacattctt
     6301 ggtaatgggg atctggggcg tggtgattgc cggcctgctg aattacttta tatttcatag
     6361 tccaatgcta agcatgctgc gatcaatgat cgccattccg cttttcgccg gtgtgaccgc
     6421 atggcaaact caaaacatga aagaaatgta ctctccctct atgccgcaag aatttgctaa
     6481 tcgcatgggc tgggcggcgg ctctaaccct gtatataagc ttcatcgcca tgtttgcaaa
     6541 catcttgaac ttgatgagcc agcgctaatc cgcgttgctc aaaacaaaat tcacaaaaag
     6601 ccgctcgaaa agggcggctt tttttaatac gcatacgccc gtgcacaaaa tccgcttttt
     6661 ctttcgcatt gttggttttg ggataaaatg caaaccatga atgaagggct tcaaagggca
     6721 gcagggatat tgcctcaaac gtatgacatg cgcggcttga aaaccctagc cgcgtttgag
     6781 cgcgattata ttgagcatat cttgcaaatt tgtcagggca acattccccg cgccgcagaa
     6841 attctcgaca ttgccccgtc aacgctctat cgaaaacgcg cggcctggga taaaaacaga
     6901 gtttaaaata cggcggtata atttttccaa acccctatca tattgactcc acttatttca
     6961 atgccgtatc gtgccttctt attttcaata aatgagagag gacctgcaat gctgaaagaa
     7021 tttaaagaat ttattgcccg cggtagtgtc atggatatgg ccgttggtat cattatcggc
     7081 gcggctttca ccgccatcgt taactcgatg gttaatgaca tgatcatgcc ggtaatcggc
     7141 gttatcacag ccggtatcga ttttggcgat ttattcatcg ctcttgatgg caacagctat
     7201 gcaaccctgg ccgccgcgaa agaagcgggc gccgcgattg tcaaatacgg tgcctttatc
     7261 aacgctgtga taaacttcct gatcgtcgct ttcgtcattt ttatcctggt taaaggtgta
     7321 aacaagctca aacgcgctga agaagaagcg cctgcgaaag tccctaaacc accaaaaagc
     7381 gaagtactac tagaggaaat ccgcgacgct ttggtcaaga aatagcggcg gcttaacaaa
     7441 tttgaaaagc gggactgcaa cgtcccgct
//
LOCUS       DAOSWR010000103         7323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6589_length_7323_cov_5.000275, whole genome shotgun sequence.
ACCESSION   DAOSWR010000103 DAOSWR010000000
VERSION     DAOSWR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6589_length_7323_cov_5.000275"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK513_07850"
     CDS             <1..356
                     /locus_tag="PK513_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOO82399.1"
                     /translation="VDEYGAMMGIVTLEDILEEIVGEIDDEHDVSVAGVRKIAGGQQY
                     MVDGTVTIRDLNREYEWGLPDEEYSTLAGLVLHEAQMIPEVGQSFMFHGFRFDIIKRQ
                     RHQITKIRVTPPKHD"
     gene            complement(334..1101)
                     /locus_tag="PK513_07855"
     CDS             complement(334..1101)
                     /locus_tag="PK513_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO82400.1"
                     /translation="MDRRRLGDTGIGISPLGLGTVKFGRNTGVKYPQGFEIPDEAALA
                     DLLTLAKDLGINMLDTAPAYGTSEERLGHLLKGRREDWVIVGKAGEEFEDGKSVYNFN
                     PEYFEMSLERSLKRLNTDYIDVLMVHSDGNDVENLSDDLIAKMQDFKARGLVRAIGAS
                     TKTVVGGLRALETMDVVMACYNADYTDEKPVLDYAAEHNKGVLLKKVLGSGHNTDVEA
                     AFRFAFVHPGTSGIIVGTINPVHLRTNIEAINRVLAA"
     gene            complement(1083..2240)
                     /locus_tag="PK513_07860"
     CDS             complement(1083..2240)
                     /locus_tag="PK513_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO82401.1"
                     /translation="MTRHTPDIVIFGAGIAGLWAFHRYKRMGYDALLLESFGIGGGQT
                     IASQGIVHSGLKYAFAGKINKLAQGISAMQNLWEAALRGEGDVDLSAAKTLSTSQYLM
                     MNNKIMGEIIKLVTSQALGNNVHEVMPEDWPQEIKSSGFHGKVVFMDEPVLDIPSVLR
                     ALAEPYKDSIRKIDTPDDPFGFLKQHNIEPKHIIFTGAASNAQIAAKEGHNHGLHTQA
                     RPLLMGMLKPAPYPLFAHLVGHTDKPVASITTHTTREGELVWYLGGGAAERKKEADPQ
                     EVYDAVRKGFAKYLPAVDLSAVQWAALPIDRIEGKSGIDGWIPDTPTIHSHENVHYCW
                     PTKLTFAPLLAERLLEKMDIQPSKTQSDWAFLPDIDYAETPWDEAQWTDGD"
     gene            complement(2297..3184)
                     /locus_tag="PK513_07865"
     CDS             complement(2297..3184)
                     /locus_tag="PK513_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO82402.1"
                     /translation="MNYQEKSYLSFHKDCEQDGQHRLYYNDWGDADANPVVCVHGLTG
                     NGHDFDYLAEDLIQNGHRVIAVDLPGRGRSDFFSNPLLYNYDQYMNDLHELLAHLGLQ
                     APGGVDWLGVSLGGLLGMRMATMKGSQIKRLILNDVGPMVPKEVLNFIHQVISQTYVF
                     DTVADFEARMRATRGLTWGPVTDEQWAHMAKYNARATDDGRITYAYDPQIAVVFEHAP
                     IGDVDLWPCWDKITCPTMVLQGADSLLLTSEILDDMRKSGPSFDLTVFEGCGHVPSLM
                     ADNQIEIVRGWLKTTLLSS"
     gene            complement(3223..5955)
                     /gene="secA"
                     /locus_tag="PK513_07870"
     CDS             complement(3223..5955)
                     /gene="secA"
                     /locus_tag="PK513_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOO82403.1"
                     /translation="MLGLATKIFGSSNERALKAYHKQIAPINALEPRYEAMSDTELQA
                     QTGVFRQRLENGEKLESLTHESFAVVREAAKRTLGLRPYDVQLIGGLVLNDGRIAEMK
                     TGEGKTLMSTLSVYLNALGGKGVHVVTVNDYLAKRDAEWMGAIYNFLGLSVGCITHDV
                     HPDERRSAYACDVTYGTNNEFGFDYLRDNMKFSLKQMVQRDFNFAIVDEVDSILIDEA
                     RTPLIISGPSEAATDLYVAVDKLIPSLQNEDYDMDEKHKTVALTDEGTEHIERLLEQN
                     GLLDGSSMYDIQNISLVHHVNQALRAHKLFLIDKDYIVKDGRVMLIDEFTGRMMEGRR
                     LSEGLHQAIEAKEGVDIQTENQTLASITFQNYFRMYPKLSGMTGTALTEAAEFAEIYK
                     LGVVSIPTHLPVTRIDYDDKIYKSLDEKEDAIVALIKDCYERKQPVLVGTVSIEKSET
                     LSERLKKEKIKHNVLNARYHEQEAHIVAQAGRPGAVTIATNMAGRGTDIKLGGNAEMR
                     IEAEIGEAVDPVKDKKAIEKIEAEVAADKQTVIDVGGLYIIGTERHESRRIDNQLGGR
                     SGRQGDPGASMFFLSVEDDLMRIFAGDKLEGLLANRKIGLQEGEALEHPLISKMLERA
                     QAKVESMHFEIRKNLLKFDDVMNDQRKVIYEQRREIMEDETIEDLAIDMRHQTIEDIV
                     NATIPAGSYAEKWDVDTLHMECQRVLGLDLPVQDWAREEGIAEGEIMERVMDESDKKM
                     AAKVAHYGAQMWRQLEKSFVLQLLDQHWKEHLLNLDHVRQGINLRAFGQKDPLNEYKA
                     EAFAAFEQMLGAMNETITQTLCLVEINVEARPGDLMPGQEQEMRETRQDPALMGTGQQ
                     AGGSTEGNVRPMTQKRAFDKDDSTTWGKVQRNASCPCGSGKKFKHCHGQLARKA"
     gene            6119..7153
                     /locus_tag="PK513_07875"
     CDS             6119..7153
                     /locus_tag="PK513_07875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO82404.1"
                     /translation="MLKVKSSNVRKNERGSAAIIALAVLVVIAIGAVAFLSGKIGYDD
                     EAATTQTASSEPASGEQAASQGETKAATAAQEAPAEQPKMPEIKPGNPVVAKIGDKEV
                     SRLDVFNFIQTLPPQTRQMPIDQLFPLALDQVVNTQIIGKKVSTAKLDDDPAVKQQLD
                     LAKEQIVRGVFIQKQVEKAMTDERLKEVYDEYVKNFPEVPEVKTAHILVKDEALAKDI
                     LKQLNEGGDFAVLAKEHSIDATKDNGGSLGYISRQDQVIPEFLEAAFALKDGGISKEP
                     LKSEFGYHIIKVEDERMRPPASFEQAKPFLASQIQAQVLNELMSSWRADAKVEVFDIN
                     GDAIEPAAGE"
BASE COUNT         1819 a         1994 c         1665 g         1845 t
ORIGIN      
        1 tggttgatga gtatggtgcc atgatgggta tcgtaacgct tgaagacatt cttgaagaaa
       61 ttgtcgggga aatcgatgac gagcatgatg tcagcgtcgc aggtgtgcgc aaaattgccg
      121 gcggacaaca atatatggtc gatggcacgg tcacaattcg cgatcttaac cgcgaatatg
      181 aatgggggtt gcctgatgag gaatattcaa cattggccgg tttggtcttg catgaagcgc
      241 aaatgatccc cgaagtcgga caaagtttta tgttccacgg ctttcgcttc gacatcatca
      301 aacgccagcg tcaccagatc accaaaatcc gcgtcacgcc gccaaaacac gattaatcgc
      361 ctcgatattc gtccgtaaat gcacaggatt aatcgtaccc acaataattc cgctggtgcc
      421 gggatggaca aaggcaaaac ggaacgctgc ttcaacatcc gtgttatgcc cgctgcccag
      481 cactttcttc agcaagacac ctttgttatg ttccgctgcg taatcgagca ctggcttttc
      541 atccgtataa tcggcattat aacatgccat caccacgtcc atcgtttcga gcgcgcgaag
      601 gccgcccact acagttttgg ttgaggcacc aatcgcccgc accaacccgc gcgctttaaa
      661 gtcctgcatc ttggcgatca aatcatcgga taaattctcc acgtcattgc catccgaatg
      721 caccatcagc acatcgatat aatcggtatt taggcgcttg aggctgcgtt caagactcat
      781 ttcaaaatac tcagggttaa aattgtaaac ggacttgccg tcttcaaatt cttccccggc
      841 cttaccaaca atcacccagt cttcccgccg gcccttcagt aaatgcccta gccgttcttc
      901 acttgttcca taggcagggg cggtatcaag catattgatt cccagatctt tagccagcgt
      961 cagcaaatcc gccaatgccg cctcatcggg aatttcaaag ccttgcggat atttcacgcc
     1021 cgtattgcgg ccaaacttca ccgtccccag ccccagcggg gagatgccta tgccggtatc
     1081 acctagtcgc cgtctgtcca ctgcgcctca tcccacggtg tttcagcgta atcaatatcg
     1141 ggcagaaagg cccagtcgct ttgcgtcttc gaaggctgaa tatccatctt ttccagcaat
     1201 cgttcagcca agagcggtgc aaaggtcagt tttgtcggcc agcaataatg gacgttttca
     1261 tggctgtgga tggtcggcgt atccggtatc cacccgtcaa tcccggattt tccttcaatc
     1321 cggtctatgg ggagcgccgc ccattgcacg gcggaaagat cgacagcagg cagatatttc
     1381 gcaaagcctt tgcgcaccgc atcgtagact tcttgcggat cggcttcctt cttgcgttcc
     1441 gctgcgccgc cgcccagata ccataccagt tcgccttcac gcgttgtatg cgtggtgatg
     1501 ctcgccacag gtttgtccgt atgcccgacc agatgcgcaa ataaagggta gggggcgggt
     1561 ttaagcattc ccatcaacaa aggacgtgcc tgcgtatgca gaccgtggtt gtgcccttcc
     1621 ttggccgcaa tctgcgcatt gctcgccgca ccggtaaaga tgatatgctt gggctcgata
     1681 ttgtgctgct tcaaaaatcc aaacggatcg tcgggcgtgt caatcttgcg aatcgaatcc
     1741 ttgtaaggct ccgccaaagc ccgcagaacc gaaggaatat ccagcaccgg ctcatccata
     1801 aacacgacct tgccgtgaaa cccgctactc tttatctctt gcggccagtc ctctggcata
     1861 acttcatgga cattattgcc cagcgcctga gaggtcacga gcttgatgat ctcgcccatg
     1921 atcttgttat tcatcatcag atattgcgaa gtcgaaagtg ttttcgccgc gcttaagtct
     1981 acatcgcctt cgccgcgcag cgccgcttcc caaaggtttt gcatggcgct gatgccttga
     2041 gccagcttgt tgatcttgcc cgcaaaggca tatttgaggc cggaatgaac aattccctga
     2101 gaagcaatcg tctgcccgcc gccaataccg aaggattcga ggagcagcgc gtcataaccc
     2161 atgcgtttat agcgatgaaa cgcccacagc cccgcaattc ccgcgccaaa aatcacaatg
     2221 tctggagtgt gtctggtcat actcattatt tataggtcat cccgagtgta gtcgaggggt
     2281 cttttcttta ttttttttac gaagataaca atgttgtttt aagccagccc cgcacaattt
     2341 cgatctgatt atccgccatc agggacggca catgcccgca gccttcaaag acggtgagat
     2401 caaacgaagg cccgctttta cgcatatcat caagaatttc tgaagtgagc agcagcgaat
     2461 cagccccctg taaaaccatc gttggacagg tgattttatc ccaacacggc cacaaatcga
     2521 catcaccaat cggtgcatgc tcaaatacca ccgctatttg cgggtcatac gcatacgtaa
     2581 tccgcccgtc atcggttgcc cgcgcattat atttggccat atgcgcccat tgttcatccg
     2641 taaccggccc ccaggtaagc ccccgcgttg cacgcatacg cgcttcgaaa tcggcaaccg
     2701 tatcaaaaac atatgtttgt gaaataacct gatgaataaa attgagtacc tctttcggca
     2761 ccatcggccc gacatcgttg agtatcagcc gcttgatctg gctgcctttc atcgtcgcca
     2821 tccgcatgcc gagcaacccg cctaacgaca cgccaagcca gtcaacaccg cccggcgcct
     2881 gcaaaccaag atgtgccagc aattcatgca aatcattcat atattgatcg tagttataaa
     2941 gcagggggtt gctaaaaaag tcgctgcgtc cgcgccccgg caaatcaaca gcaatcaccc
     3001 ggtggccgtt ttggattaga tcttcggcca gataatcaaa atcgtgtccg ttaccggtta
     3061 atccatggac gcaaacaacc gggttggcgt ctgcatcacc ccagtcgttg taatacagcc
     3121 gatgctgccc atcttgctcg caatctttgt gaaaactcag atatgatttt tcctgataat
     3181 tcatagggca actttaatcg tcatcccggg cgcttgtcca ccctaagcct tacgcgccaa
     3241 ttgcccgtga cagtgcttga atttcttgcc tgaaccgcaa gggcatgacg cgttgcgctg
     3301 aactttgccc catgtcgttg aatcgtcttt atcaaatgcg cgtttctgcg tcataggccg
     3361 aacatttccc tctgtacttc cgccggcttg ctgccctgtg cccataagcg ccgggtcttg
     3421 ccgcgtttca cgcatttctt gctcttgacc cggcataagg tcaccaggac gcgcctcaac
     3481 attaatttca accagacaca gcgtttgcgt gatggtttcg ttcatcgcgc cgagcatttg
     3541 ttcaaaagcc gcgaaggctt cagccttata ttcattgaga ggatctttct gaccaaaagc
     3601 gcgcaaattt atcccttgac gtacatggtc gagattaagc aaatgctctt tccagtgttg
     3661 atcaagcaat tgcaacacga aggatttttc aagttgacgc cacatctgcg cgccgtaatg
     3721 cgcaactttc gccgccattt tcttgtccga ttcatccatc acgcgttcca tgatttcgcc
     3781 ttccgcaata ccttcctcgc gtgcccaatc ctgaacaggt aaatcaagcc ccaacacccg
     3841 ctggcattcc atatgcagcg tatccacatc ccatttttcg gcatagctgc ccgctggaat
     3901 agtggcattc acaatgtctt caatagtctg gtgacgcata tcaatggcaa gatcttcgat
     3961 cgtttcatct tccatgatct cgcggcgctg ctcataaatc actttgcgct gatcgttcat
     4021 cacatcatcg aacttcaaaa gatttttacg aatttcaaaa tgcatagact cgactttagc
     4081 ctgcgccctc tccagcattt tagaaatcaa gggatgctcc agcgcttcgc cttcctgcag
     4141 gccaatttta cgattggcca gaagcccctc aagcttgtca ccagcaaaaa tgcgcatcaa
     4201 atcatcttct acagacagga agaacattga tgctccgggg tcgccctgac gaccagatcg
     4261 gccaccaagc tgattatcaa tccgccgcga ttcatggcgc tctgtaccga tgatatacag
     4321 cccgcctaca tcaataacgg tctgcttgtc agcagcgact tcagcttcaa ttttttcgat
     4381 cgctttttta tctttgaccg ggtcaaccgc ctcaccaatc tctgcctcaa tacgcatctc
     4441 cgcattgcca cccagcttaa tatcagttcc acggcccgcc atgtttgtgg caatcgtcac
     4501 cgcgccgggg cgcccggctt gcgcaacaat atgcgcttct tgttcgtgat aacgcgcatt
     4561 caaaacatta tgcttaattt tctctttctt gaggcgctcg gaaagcgttt cagatttttc
     4621 aatcgaaacc gttccgacca aaaccggctg tttgcgctcg taacaatcct taatcaacgc
     4681 aacaatggca tcttctttct catccaaaga tttataaatc ttgtcgtcat aatcaatccg
     4741 tgtcaccggc aaatgcgttg ggatagacac cacgcccagt ttgtaaattt cggcaaattc
     4801 cgccgcttcg gttagcgccg tgcccgtcat gcctgagagc ttcggataca tgcggaagta
     4861 attctggaat gtgatcgaag ccagcgtttg gttttcggtc tgaatatcga ccccttcctt
     4921 tgcctcaatg gcttggtgca agccttcgga caggcggcgc ccctccatca tccggcccgt
     4981 aaactcatca atcagcatca cgcggccatc cttgacgatg taatccttgt cgatcaggaa
     5041 cagcttgtgg gcgcgcaaag cctgattcac atgatgcact agcgagatat tctgaatgtc
     5101 atacatgctg ctgccatcaa gcaagccgtt ctgctccagc aagcgttcga tatgctctgt
     5161 accttcatcg gttagcgcaa ccgtcttgtg cttttcatcc atgtcataat cttcgttttg
     5221 caaagacggg atgagcttat caacggccac ataaaggtca gtcgccgcct cggatggccc
     5281 ggaaataatc agcggcgtac gcgcttcgtc aatcaggatc gaatcaacct catcgacaat
     5341 cgcgaaattg aaatcacgct gaaccatttg cttcaggctg aatttcatgt tgtcacgcaa
     5401 ataatcaaaa ccaaattcgt tgttcgtgcc gtatgtcaca tcgcatgcgt aggcgctccg
     5461 gcgctcatct ggatgaacat catgagtgat acagccaacg ctgaggccca aaaagttata
     5521 aatcgcgccc atccattcgg catcacgctt ggccagataa tcattgaccg taacaacgtg
     5581 gacgccttta ccgcccaagg cattgagata aacagacagg gtagacatca gtgttttccc
     5641 ttcaccggtt ttcatctcgg caatgcgccc gtcattcaaa accaatccac cgataagctg
     5701 tacgtcatag ggacgcaggc ccaatgtacg cttggccgct tcgcgaacaa ctgcgaaaga
     5761 ttcatgggta aggctctcaa gtttttcacc attttcaagc cgctggcgaa agacgccggt
     5821 ttgggcttgc agctcagtat cactcatcgc ttcataacgc ggctcaagcg cattaattgg
     5881 cgcaatttgt ttatgatacg cttttaaggc acgctcattc gatgagccaa aaattttggt
     5941 ggccagacct aacaacggca ttctccttag atgtaagttg ttttgatata aagctccggg
     6001 cgggcggcgt caatgattgt tgaaaagctg cgttcaatca gttgcaaata gcttttcctt
     6061 tcgttagtct ccattcattc aagcaatatc atttttggaa gaagagagaa ccgaaacaat
     6121 gcttaaagtt aaaagctcga acgtaagaaa aaatgaacgt ggcagtgcgg ctataattgc
     6181 tttggcggtc ctggttgtga tcgccattgg tgctgtggca tttctgtctg gtaaaattgg
     6241 ctatgatgat gaagcagcga caacgcaaac agcgtcttcc gaacctgcaa gtggcgaaca
     6301 agccgcatcg caaggcgaga cgaaggcagc caccgcagcg caagaagcgc cagctgaaca
     6361 gccaaagatg cctgaaatta aacccggcaa tccagttgtt gccaagatcg gtgataagga
     6421 agtcagccgt ttggatgttt ttaatttcat ccagacccta ccgccccaaa cccgtcagat
     6481 gccgattgat caactctttc cattggcgct ggatcaagtc gtaaacacac aaattattgg
     6541 caagaaagtc tcaacagcaa aactggatga tgatccagcc gttaagcagc agttggatct
     6601 ggcaaaagaa caaatcgtgc gcggcgtgtt catccaaaag caggttgaaa aagccatgac
     6661 ggatgaacgc ctgaaagaag tttatgacga atatgtgaag aactttcctg aagtgcctga
     6721 ggttaaaact gcccatatcc ttgtaaagga tgaagcgctg gccaaagata ttctgaagca
     6781 actgaatgaa ggtggtgact ttgcagtcct ggcgaaagaa cactctattg atgcgacgaa
     6841 agacaatggt ggcagccttg gttacatctc tcgtcaggat caggtcattc cggaattttt
     6901 ggaggcggct tttgctttaa aagacggagg gatcagcaaa gagcctctga aatctgaatt
     6961 cggctatcac attatcaagg ttgaagatga gcgcatgcgc ccgccggcaa gctttgaaca
     7021 agccaagccg tttctggcgt ctcaaattca ggcgcaagtt ttgaacgaac taatgtcatc
     7081 atggcgtgca gatgccaaag ttgaagtttt tgacatcaac ggcgatgcga tcgagcctgc
     7141 tgctggtgaa taaattatat taagcaaatt agaaaggccg tgagcgcata tgcttgcggc
     7201 ttttttattg aaggaaaagc catgcattgt gacgaagctc taaagttacc aaagccgacg
     7261 ctgccgaatc ttccgattat tctggtcacc gcagcagcgc ttatcaatcc gcgcggcgag
     7321 att
//
LOCUS       DAOSWR010000104         7200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6748_length_7200_cov_5.155773, whole genome shotgun sequence.
ACCESSION   DAOSWR010000104 DAOSWR010000000
VERSION     DAOSWR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_7200_cov_5.155773"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK513_07880"
     CDS             <1..504
                     /locus_tag="PK513_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HOO82405.1"
                     /translation="TGTNYFDGGWHQVVITAVHADTLKCYVDGVLKLTQAITSSQVNS
                     NIDLPEYGIGAVNEIDNGVYNHFFEGSIDDVRIWDRALSQDEAEGLFCTGSVGKIDYN
                     TTDEIMQFCSDVGRHDMSSTNPGTGGGGCSATGTIAAGVAGTWQYDTTNKKMVFCDGT
                     NWRNAGE"
     gene            632..708
                     /locus_tag="PK513_07885"
     tRNA            632..708
                     /locus_tag="PK513_07885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:Met,seq:cat)
     gene            807..1295
                     /locus_tag="PK513_07890"
     CDS             807..1295
                     /locus_tag="PK513_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82406.1"
                     /translation="MEIQQTPEKHPVLGGKAYVYKRGRSSYWQAAAYLRGYNYRTSTK
                     ETYLHEAIHYAEEWYFNLRGQASIGVLETPAEKQEKEKVLTFRDVADKFIQEYSVITE
                     GQRSPAWIRGHSDRLRVHILPFLGDLPIKEITGGKVQEYRVYRITPQTEKNPQLRSHQ
                     TL"
     gene            complement(1243..1953)
                     /locus_tag="PK513_07895"
     CDS             complement(1243..1953)
                     /locus_tag="PK513_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82407.1"
                     /translation="MALYKIEDRKIKAIERTTFAHQNIKERDDLQKMLKENIEIISPD
                     TLIVAEEFGEWEDSRRRIDLLGLDKDANLVVIELKRTEDGGHMELQSIRYAAMISTLT
                     FEKLVAIYGHYLKANQIDNDPTSALLEFLDWEEPDEDNFAQEVKIILASAEFSKELTT
                     SVMWLNDFGLDIKCVRMHPYVSGILDIQTVIPIPEVADYQIKIREKKQKERASRENSR
                     DFTKFDVSVAEDSFLFAG"
     gene            2123..2377
                     /locus_tag="PK513_07900"
     CDS             2123..2377
                     /locus_tag="PK513_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO82408.1"
                     /translation="MSDLARSPEQIGNIIRRVRKKRGMSQSALGEKTGLRQATISQIE
                     KGHPAAKLETILAVLSALGLEFQINGRTSFDPFGAANKKP"
     gene            2521..3570
                     /locus_tag="PK513_07905"
     CDS             2521..3570
                     /locus_tag="PK513_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HOO82409.1"
                     /translation="MIKTNHLNITTDILSLISAIDEFKGAWRALGTLAPERLSALRRV
                     ATIESIGSSTRIEGSKLSDRDVEKLLANLEIKSFETRDEQEVAGYADVMETVFQSWSE
                     IPITENHIKQLHRDLLAHSQKDERHRGNYKTSSNSVAAFDEAGKQVAIVFETATPFDT
                     PRLMEELVTWARGEFESKTLHPLLVIAIFIVVFLEIHPFQDGNGRLSRVLTTLLLLRA
                     GYAYVPYSSLESIIEKSKESYYLALRQTQGTIRTDEPNWQPWILFFLRSLQGQVQALS
                     KKVEREKIVLAALPELSVQILEQAREHGRVTIGDMIKLTGTSRNTLKEHFRALVEKGH
                     LVMHGHGRGVWYSLA"
     gene            complement(4030..5070)
                     /gene="arsB"
                     /locus_tag="PK513_07910"
     CDS             complement(4030..5070)
                     /gene="arsB"
                     /locus_tag="PK513_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOO82410.1"
                     /translation="MSTAQSPLGIFERYLSLWVGLCIAAGVGLGLLVPGAFQAIASLE
                     YANVNLVVAVFIWVMIYPMMVNVDFASLKDVGKKPKGLCITLVINWLVKPFTMAALGI
                     LFFEHIFAGLVEPQDAKEYIAGMILLGVAPCTAMVFVWSQLVRGDANYTLVQVSINDI
                     IMIFAFAPLVALLLGVTDIIVPWETLLLSVILYVLIPLAAGYITRKKLDGSGNHERIA
                     KFTAKVKPFSIMGLLATVVLLFGFQAGTIIEKPMVIALIAIPLLIQSYGIFAIAFGWG
                     YFWRVPFSTAAPAALIGTSNFFELAVAVAISLFGLNSGAALATVVGVLVEVPVMLSLV
                     AFANKTRQHFHP"
     gene            complement(5067..5477)
                     /locus_tag="PK513_07915"
     CDS             complement(5067..5477)
                     /locus_tag="PK513_07915"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOO82411.1"
                     /translation="MKDILVLCTGNSCRSVMAEALINQLSAGRYKAVSAGSKPAGYVH
                     PKSIETMKRNGIVAGTPRSKSWDEFEGKNFDLVITVCDTAAAESCPVFLGKHEKLHWS
                     TPDPAAATGNEEEINAAFDEAFNLLKTRIEKELL"
     gene            complement(5479..5928)
                     /locus_tag="PK513_07920"
     CDS             complement(5479..5928)
                     /locus_tag="PK513_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HOO82412.1"
                     /translation="MKISEVKEALETLDAVNFKLPDGSTLPPHFHVTEVGLVTKHFID
                     CGGTERTETVANFQLWEAGDYDHRLAPQKFLHILNLCKKVLGDGDHEIEVEYQQNTIG
                     KFGLRFEDGDFILTNKQTACLAQDACGIPDKKPKEASACCAPKSGCC"
     gene            complement(5944..6294)
                     /locus_tag="PK513_07925"
     CDS             complement(5944..6294)
                     /locus_tag="PK513_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO82413.1"
                     /translation="MDIQNATIMFDALSQETRLGAFRLLVKAGPEGLPAGAISDELGT
                     PHNTMSFHLNHLSNAGIVTSRREGRSVIYAANFEVVRDLIGFMVKDCCSDEFASIRED
                     KKKGCSIIELAKCC"
     gene            complement(6352..6840)
                     /locus_tag="PK513_07930"
     CDS             complement(6352..6840)
                     /locus_tag="PK513_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82414.1"
                     /translation="MVVYPEKVIIALRPLGMAGLLHEMMPDAKIKKGQPTENTPLEIT
                     IPIKLKLHGGRKYITTPDGRDLAIARKPKYENNMIRAVGRGHQYLEMLEADPDLTIMK
                     LAKREKLDHGYIAKTVRMTQLAPDIIEAILNGRQPYSLNLTDFMKPFPNSWEEQRQHF
                     GF"
     gene            complement(6852..>7200)
                     /locus_tag="PK513_07935"
     CDS             complement(6852..>7200)
                     /locus_tag="PK513_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOO82415.1"
                     /translation="AAHFDAASRPPDLSLPAQLVGELKEKGYQSKSWVARSGRFHPGK
                     PITKSSIYKILDNPVYIGKISHKGTIYEGEHVGFIDPADFEKAKEIRETRAPNSSPLR
                     CTQAQYLLRGLPN"
BASE COUNT         1867 a         1642 c         1692 g         1999 t
ORIGIN      
        1 accggcacga attattttga tgggggctgg catcaggtgg tgattacagc tgtacatgct
       61 gatactctga aatgctatgt tgatggtgta ttaaaattga ctcaggccat tacaagttca
      121 caggttaata gtaatattga ccttccagag tacggaattg gtgcagtgaa tgaaattgac
      181 aatggtgttt acaaccactt tttcgaaggc agcattgatg atgtgcgtat ttgggaccgg
      241 gctctttcgc aggatgaagc ggaaggactc ttctgcacag gctccgtagg taaaattgac
      301 tacaacacaa cggatgagat catgcaattt tgttcggatg tggggcggca cgacatgagt
      361 tcaaccaacc ccggcacggg cggcggcggg tgtagcgcga ccggaacgat tgccgcaggc
      421 gtcgccggga cgtggcaata tgataccacc aataagaaaa tggttttttg tgacgggaca
      481 aactggcgca atgctgggga gtaagccgct tttgtcctga ttctatctgc atttcgcttt
      541 tattttctgg ttcagggctt gattttcgtt tgggtggggg ctataaccct gtctctactt
      601 ggtgatcgta ttgtgtgtca ccagttgttg acgcgggatg gagcagcccg gtagctcgtc
      661 aggctcataa cctgaaggtc gtaggttcaa atcctactcc cgcaaccaat aaatcaatga
      721 cttagcaaca cttctttaac cataatactg ctaaaattga cttacacact acttacacac
      781 ttttgtactg tgaaaggcat cgtattatgg agatacagca gacaccagaa aagcatccag
      841 ttttaggcgg taaagcctat gtttataaac gcgggcgcag cagctattgg caagccgccg
      901 cttatctgcg cgggtataat taccgtacat cgacaaaaga aacatatctg catgaagcga
      961 tacactacgc cgaagagtgg tatttcaatc tgcgcggtca ggcgagcatt ggcgttttag
     1021 aaacgcccgc cgagaagcag gaaaaagaaa aagttctaac cttccgcgat gtcgcggaca
     1081 aattcattca agaatattcc gttatcaccg aaggccagcg tagccctgca tggattcgag
     1141 gccattcaga ccgtttgcgc gttcatatcc tgcccttcct cggcgatctg cctattaaag
     1201 aaatcacagg cggcaaggtg caggaatatc gggtttatag aatcaccccg caaacagaaa
     1261 agaatcctca gctacgctca catcaaactt tgtgaaatct cttgagtttt cacgtgatgc
     1321 acgttctttt tgctttttct ctcttatttt aatttgataa tcagccactt ctggaattgg
     1381 tataaccgtt tgtatgtcca aaattccgct aacgtaagga tgcattctta cgcattttat
     1441 atcgaggcca aaatcattaa gccacattac cgatgttgtt aattctttag aaaattccgc
     1501 tgaggctaga atgattttta cttcttgagc gaaattatct tcatccggct cttcccaatc
     1561 taaaaattct aaaagggcgc ttgttgggtc gttgtctatt tgatttgctt ttaaataatg
     1621 cccgtaaata gctacaagct tttcaaatgt gagagttgaa atcatcgcgg cataacgaat
     1681 gctttgaagc tccatatggc caccatcttc tgtgcgtttt agttctatta caactaaatt
     1741 tgcgtcttta tctaaaccca gtaaatcaat acgccttcta ctgtcttccc attcaccaaa
     1801 ttcttcggcc actataagcg tgtctggaga aattatttct atattttctt tgagcatttt
     1861 ttgaagatca tcgcgctctt taatgttttg gtgcgcgaat gtggtgcgtt ctattgcttt
     1921 aatttttctg tcttcaattt tatacagtgc catttgtaga gcctaatcaa ttttattttc
     1981 agaataacta tatggattat caagaatgct ttgaatagga aaattttgaa tcgggtataa
     2041 tcgggtatga aatcaggtat tttttattta tacgccaatt cgtatatttt cattttatac
     2101 attttagcgt ataatttttc ttatgtctga tttagcccgt agcccagaac aaattggaaa
     2161 tataattcgc cgcgtacgaa aaaaacgcgg catgagtcaa tctgcactag gagagaaaac
     2221 cggacttcgc caagccacaa tttcacagat tgaaaaaggc catccggcgg caaagctgga
     2281 aacgattttg gccgtgcttt ctgctctggg tttggaattt caaattaacg gcagaacgtc
     2341 ttttgatcct tttggtgctg caaataaaaa gccttaattg cttgctcaat tttcattttt
     2401 aacgcccctc agggtgtaaa cctgacgggc ataggatgta agagcatccc tagaaatggg
     2461 tgtaaatggg tgtattattg gcttgttaaa tgggtgtaaa tgggtgtatt attggttgtt
     2521 atgattaaaa ccaaccattt aaatatcacc acagatatac tttctttgat ctcggccata
     2581 gacgagttta agggcgcttg gcgtgccttg ggcactttag cgccggaaag actctcagcg
     2641 ctgcggcgcg tggctacgat tgaaagcatt ggctcttcaa cgcggattga gggcagcaag
     2701 ctttctgatc gtgatgttga aaagcttctg gccaatttag aaatcaaatc ttttgaaact
     2761 agagatgagc aagaagttgc tggttatgcc gatgtcatgg aaacggtttt tcagtcttgg
     2821 tcagaaatac ccatcacgga aaaccatata aagcagcttc atcgtgattt attggcgcat
     2881 agccagaaag acgagcgcca tcgtggcaat tataaaacaa gctcaaattc agttgccgct
     2941 tttgatgagg caggaaaaca agttgctatt gtttttgaaa cggccacgcc ctttgatacg
     3001 ccgaggctga tggaagaatt ggttacgtgg gcgcggggag aatttgaaag caagactctg
     3061 caccccttgc tggtgattgc aattttcatt gttgttttct tggagataca cccgtttcaa
     3121 gacgggaatg gaagactgag ccgcgttctt acaacgctcc tacttttgcg cgctggctat
     3181 gcttatgtgc cgtattcatc gctggaaagt attattgaga aaagcaaaga gagctattac
     3241 ctcgcgctca gacaaacgca aggtacaatc agaacggatg aaccaaactg gcagccgtgg
     3301 attttgtttt tcttgcgatc attgcaaggc caagtgcaag cgctttcaaa gaaagtggag
     3361 cgcgagaaaa tcgttcttgc ggcattgccg gagctttccg ttcaaattct tgaacaggct
     3421 cgtgaacatg gccgcgtgac gattggtgac atgattaagc ttacaggcac aagccggaat
     3481 acgctcaaag aacatttccg cgctctcgtt gaaaaagggc atcttgttat gcacggccat
     3541 ggccgcggcg tgtggtattc gttggcataa tttcacgatt tttctaaact ttaacgccct
     3601 tcagggcgaa aaccggaagg gcttgactta cacacttact tacacacata atgaaaacgc
     3661 aagaaaagtt ttagtattat caatgtgtta aagagatttg gtttgcgctc ataacctgaa
     3721 ggtcgcaggt tcaaatcctg ctcccgcaac caattttcta aatccaaact ctctctaagc
     3781 cttgtaatcc cttgcaaaag ggcagagttt ttgggctttt gcgtccaaag ctaaagggtg
     3841 tgtgcccctt caaaaagcca tttatacccc cgttttctct ctaaataccg atttttctcc
     3901 aaagctgtag aggcgattac aaaaacgccc ttcaggtttt tgtcgttatt tatcaatgtg
     3961 ttgattgttc tgggaaatta actgttttca ggtcgcagcg taaaccgatt tagacgtgag
     4021 agaaaatatt tacggatgaa aatgttgtcg cgttttattg gcaaaagcca ccagagagag
     4081 catgacaggc acttcgacca gaacgcccac gaccgtggcc aaagctgcgc cggagttaag
     4141 gccgaacaga ctgatggcca ccgccacggc cagttcaaag aaattggatg tgccgatcag
     4201 ggcggcaggt gcggctgtgc tgaagggaac acgccagaaa tagccccatc caaaggcaat
     4261 ggcaaaaatg ccgtaacttt gaataagcaa gggtatagca atcagggcaa tgaccattgg
     4321 cttttcaatg atcgttcctg cttgaaagcc gaaaagcaaa acaaccgtag ccagcaaacc
     4381 catgatagaa aacggtttta ccttagctgt gaatttggca atgcgctcgt ggttgcctga
     4441 accatccagc tttttgcggg tgatatatcc cgccgccaag ggaataagca catagaggat
     4501 gacggagagc agcaacgttt cccacggcac gataatatcg gttacgccta gcaagagcgc
     4561 taccagcggc gcaaaggcga aaatcataat gatatcgttg atcgacactt gcaccagcgt
     4621 gtaattggca tcgccgcgca ctagctgact ccatacaaac accatcgcgg tgcagggtgc
     4681 aacgccgagc aggatcatcc cagcgatata ttccttggcg tcctgcggct cgaccagtcc
     4741 agcaaaaata tgctcaaaga acagaatccc caatgcggcc atcgtaaagg gcttaaccag
     4801 ccagttaatc accagcgtaa tacatagccc cttgggcttt ttgccgacat ctttaaggct
     4861 ggcgaaatcg acattgacca tcatcgggta aatcatcacc catatgaata cggcaacgac
     4921 cagattaacg ttggcatatt caaggctggc aatagcctga aatgcgcccg gcaccagcag
     4981 gccaagccca accccggcgg caatgcacag cccgacccat aacgagagat agcgttcaaa
     5041 aatgccgagg ggtgattgtg ctgtgctcat aataattctt tttctattct ggtttttagt
     5101 aagttaaacg cctcgtcaaa ggcggcgtta atttcttctt cgttgcccgt ggcggcggca
     5161 gggtcaggcg tactccaatg caacttttca tgcttgccca gaaatactgg gcagctttca
     5221 gctgcggcgg tatcacaaac cgtaatcacc agatcaaaat ttttaccctc aaattcatcc
     5281 caagacttgc tgcgtggtgt cccggcaaca atgccattgc gtttcatggt ttcaatggac
     5341 ttgggatgca catatccggc aggcttactg cctgcactca ccgctttata ccgccctgcg
     5401 ctaagctggt tgatcagcgc ctcggccatc acggagcggc atgagtttcc ggtgcatagg
     5461 acaagaatgt ctttcatgtc agcagcatcc gcttttgggg gcgcagcagg cgcttgcctc
     5521 tttgggcttt ttatcgggta tcccgcatgc atcctgcgcg aggcaggcgg tttgtttgtt
     5581 ggtcaggatg aaatccccat cttcaaaacg caggccgaat ttgccgatgg tgttttgctg
     5641 gtattcgacc tcgatttcgt gatccccatc gcccagcacc tttttacaaa ggtttagaat
     5701 atgcagaaat ttttgtggcg caaggcgatg atcgtaatct ccggcctccc aaagttggaa
     5761 attggctacg gtttcggtgc gctccgtccc gccgcaatca atgaaatgct tggtcacaag
     5821 cccgacttct gtgacatgga aatgtggcgg cagggtggaa ccatccggca atttgaaatt
     5881 cacagcgtcc agcgtttcaa gcgcctcttt cacttcagat attttcatgt cgtttccttt
     5941 cctttagcaa cacttggcca gctcgataat tgagcagccc tttttcttat cttcgcggat
     6001 actagcgaat tcatcgctgc agcaatcctt gaccataaat ccgatcagat cgcgcaccac
     6061 ttcgaaattc gccgcatata taacggaacg cccttcgcgg cgcgatgtca caatccccgc
     6121 attagataaa tgattgaggt gaaacgacat ggtgttgtgc ggcgtaccaa gctcatcgct
     6181 gattgcgcca gcaggcagcc cttccggccc ggccttgacc aatagacgga aagccccaag
     6241 gcgggtttcc tgagaaaggg cgtcaaacat aatcgttgcg ttttgtatgt ccatatgtcg
     6301 agaataatcg acatatacaa gaaatgtcaa tatcttttgg catacttctg attaaaatcc
     6361 aaaatgctgg cgttgttctt cccagctatt cgggaagggt ttcatgaaat cggtaaggtt
     6421 gaggctgtaa ggctggcggc cattcaggat ggcttcaatg atatcgggtg ctaactgggt
     6481 catgcgtact gtcttggcaa tgtagccgtg atcgagtttt tctcgcttag ccagcttcat
     6541 gatggttaaa tcaggatcgg cttccagcat ttcaagatat tgatgcccgc gcccaacggc
     6601 tctgatcata ttattttcgt atttgggttt tcgtgcaatg gccaaatcac ggccatcagg
     6661 ggtggtgata tatttcctac cgccgtgaag ttttaatttg atcggaattg ttatttccag
     6721 cggtgtgttt tctgtgggct gccctttttt gattttggca tccggcatca tttcatgcaa
     6781 aagcccggcc atccccagcg gacgcagtgc aataatcact ttttccggat agaccaccac
     6841 tttttggatg atcagtttgg caatccgcgc aaaagatatt gtgcctgggt gcagcgtaac
     6901 ggcgatgaat taggcgctct ggtttctctt atctctttgg ctttttcaaa atcggcggga
     6961 tcaataaaac cgacatgctc gccttcatag atagtgcctt tatgactgat ctttccgata
     7021 taaaccggat tatccaagat tttgtaaatc gagctttttg tgatgggttt tccgggatgg
     7081 aaacgtccgg atctggcaac ccagcttttt gattgatagc ctttttcttt taactcccca
     7141 accagttgag ccggtaggga taaatccggt ggccgggatg cggcatcgaa gtgcgcggcc
//
LOCUS       DAOSWR010000105         7160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6802_length_7160_cov_5.201830, whole genome shotgun sequence.
ACCESSION   DAOSWR010000105 DAOSWR010000000
VERSION     DAOSWR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6802_length_7160_cov_5.201830"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PK513_07940"
     CDS             <1..1547
                     /locus_tag="PK513_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOO82416.1"
                     /translation="RFAGDISLFGPYASPVGKAQFDLKRIAIPGIDEAENRYVDGTLS
                     ASYKNHVITAQTDLSGPAELKFKAKVTLPLTISPLVIPFDKPVSGNVNASVDLKALSL
                     LLGLDEHSITGRSTLDVSLSGTLNKPAITGKGNLRNGTYENLLLGTRFKDITGEVTAS
                     ENRLSLKNLEGRDTNGGIFTGSGHIDFRNIRDPQYQFALETKTLQLVNTDRMGVTASG
                     QLKTAGDMDKAEVKGQVMINQAEYYISQLVSTSSLSSFTIIEENGNGDEGANTQEKQN
                     GPDVMLNIGIEANNNVYVRGPDLETEWSGKLNVTGTAKELEIKGGLSLVRGRFQLLDT
                     PVQLSKGNVRFAGDDPANPDIDVSGTIKGRDMDAILKITGETKAPEIGLQSEPPLPED
                     EILAKTLFGKSVSQLSPVQALRIAQLMAYLSGRQEASFDPLNDIRRAIGIDTLSVGMD
                     DEKGATLSIGKYVNDRVYIGVDQGTTPGSSAVRAEIEVTDDIEVETKTNSTNESSVGV
                     NWKKDY"
     gene            1558..1686
                     /locus_tag="PK513_07945"
     CDS             1558..1686
                     /locus_tag="PK513_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82417.1"
                     /translation="MRAENVFAAIVETRYFFAAQKIIVERRLTCFYHHPVMSENSI"
     gene            1800..2153
                     /locus_tag="PK513_07950"
     CDS             1800..2153
                     /locus_tag="PK513_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO82418.1"
                     /translation="MQPETSYPAIIGRILEHHRKEAGYDQGDIAEALGLTQSAWSRIE
                     RGQSGLSLEYLVRFSEIIKTEPHKIVEDADYASKRIKADGVMVHPHVITNTDKVVAAL
                     SLAALGMMIFAILKK"
     gene            2335..2514
                     /locus_tag="PK513_07955"
     CDS             2335..2514
                     /locus_tag="PK513_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82419.1"
                     /translation="MIVRDPENHKKKLWIKERDVLEQIEAVFASIQVPKAYLFAGLTG
                     NCMLKRFLSLMPYNR"
     gene            complement(2518..2985)
                     /gene="queF"
                     /locus_tag="PK513_07960"
     CDS             complement(2518..2985)
                     /gene="queF"
                     /locus_tag="PK513_07960"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019295560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOO82420.1"
                     /translation="MTDKTIYQNLTQLGGESKIPVSPDKAVLERVPNPHKDVGYAVRF
                     TAPEFTSICPLTGQPDFAHLVLDYVPGDWLVESKSLKLFLTSYRNHGDFHEACTISIA
                     KRLQETTQAKWLRLGGYWYPRGGIPIDIFYQTGEEPKDIWIPAQDVPNYRGRG"
     gene            complement(2989..3693)
                     /gene="queE"
                     /locus_tag="PK513_07965"
     CDS             complement(2989..3693)
                     /gene="queE"
                     /locus_tag="PK513_07965"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOO82421.1"
                     /translation="MNENIRISEIFGPTIQGEGALIGQPTVFVRTGGCDYRCTWCDTL
                     HAVDSVYRHDWKPMNAKEIMNEITRLSEDQPMMVTLSGGNPAIQPLDPLITLGHEQGY
                     RFAMETQGTIAQNWFTDLDVLTLSPKPPSSGHDTNWDKFDECLKAASNKPQIALKIVI
                     FDEIDYAYAKEAAAHYPDLPLYLQPGNHKTEGPADLPGLNARLEWLAEKVTNDNTNAH
                     VLPQLHVMLWGNKRGV"
     gene            complement(3797..4153)
                     /gene="queD"
                     /locus_tag="PK513_07970"
     CDS             complement(3797..4153)
                     /gene="queD"
                     /locus_tag="PK513_07970"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOO82422.1"
                     /translation="MYTISKEYHFSASHILHGLADDHPCARLHGHNYVVEVELQSEKL
                     NSTGFVHDYRELDALKKYIDEEIDHRHLNDVLSENNTTAERIAKHFYDWCKDKWPEVS
                     AVRVKETPKTSAEYRS"
     gene            complement(4153..4860)
                     /gene="queC"
                     /locus_tag="PK513_07975"
     CDS             complement(4153..4860)
                     /gene="queC"
                     /locus_tag="PK513_07975"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009824713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOO82423.1"
                     /translation="MKTAVICSGGLDSITLAYKTAKQRTLTRLISFDYGQRHKKELKC
                     AAHHARALGVPHHIIDISAVGAALTGSALTDDVTVPDGHYAENSMKITIVPNRNAIML
                     TIAYGIASANKDDAVATAVHGGDHFIYPDCRPSFIKAFAAMQNEALAGVSNIELYTPF
                     LDKTKADIVAEGTRLGVPFIETWSCYKGGKIHCGRCGTCVERQEAFNIAGINDPTGYE
                     DPDFWIETCRIKKESGA"
     gene            complement(5178..6020)
                     /locus_tag="PK513_07980"
     CDS             complement(5178..6020)
                     /locus_tag="PK513_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO82424.1"
                     /translation="MKFVYFGYDFMLPAIQRALDEGHELTGIFSFECDNIFNFNRQCQ
                     ALAQKRNVPFIISKAEDFHLNPFLDKGVELFFAAGYPYKIPPIDPARAYAVNLHPTYL
                     PKARGLMPIPRIILENMIDAAGLTAHKMTQDFDAGDILLQEKVNLSPNESVETYSAKI
                     ALRAPELVSNLFTNLPELWNNASVQDENKATTIKPPSDHDRLLNWNESIENIARTARA
                     FGNFGSLASFDEQLWVVYNLDVWKEDHALPPGTIAARLSRETLVAAQNGFVCLKDYQP
                     ARIE"
     gene            complement(6078..6491)
                     /locus_tag="PK513_07985"
     CDS             complement(6078..6491)
                     /locus_tag="PK513_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOO82425.1"
                     /translation="MIGPINIALSGLAAANKQLTASASNIANLQTTGSLEEGEQMPYT
                     PLRAQQTAQTDNEGNGQGVTSTFVPKNDPFVPAYAPDSPFANNEGLIGMPNVDLAEEA
                     VNLNLAKIQYKANIKTIEAASELSEELFRLFDDKA"
     gene            6623..7096
                     /locus_tag="PK513_07990"
     CDS             6623..7096
                     /locus_tag="PK513_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82426.1"
                     /translation="MFFLLGVSVNAGMGFARDSIWGAPQDYTVRPFLEEAKLPHNSRW
                     ADDEWSPQDWIDSRGGSVVAVVDGFYRADIVVDQYVDDDVPVLEVGQGFLDLGAQDQR
                     RVVAFMDEVFGVTKFATPGIILVLFHRQDKSWLHGLIEGDTERIGVFDANGLQLQ"
BASE COUNT         1749 a         1473 c         1955 g         1983 t
ORIGIN      
        1 gacgctttgc cggagatata agcctgttcg gcccctatgc tagccctgta ggaaaagcgc
       61 agtttgattt aaaacgcatt gccattcccg gcattgatga ggcggaaaac cgctatgtgg
      121 acggtacgct gagcgccagc tataaaaacc atgttatcac ggcgcaaacc gatctcagcg
      181 gtccggcaga attaaaattc aaagccaaag ttactctgcc cctgacaatt tcgccgttgg
      241 ttattccttt tgataagcca gtcagcggca atgtaaatgc gagtgttgat ctcaaggctt
      301 tatctttgtt gcttggtctt gatgagcaca gcattacagg acgcagcaca cttgatgtat
      361 cattatctgg aactctcaat aaaccagcta ttacgggcaa gggaaatttg cgaaacggaa
      421 cgtatgagaa tttattgctc ggcacacgat ttaaagacat tacaggtgaa gttacagcga
      481 gcgaaaaccg tttgagcctt aaaaatcttg aaggccgtga taccaatggc gggatattca
      541 ccggcagcgg ccatattgat ttcaggaata tccgcgaccc gcaatatcag tttgcgctgg
      601 aaacaaaaac cttgcagctt gtgaacaccg atcgcatggg tgtcaccgca agcggtcagc
      661 ttaaaacggc cggggatatg gacaaggccg aggttaaggg gcaggttatg atcaatcagg
      721 cggaatatta tatctcacaa ctggtcagca ccagttcact cagtagcttt acgatcattg
      781 aagaaaacgg caatggggat gaaggtgcaa acactcagga gaaacaaaat ggtccggatg
      841 taatgctaaa tattggcatt gaagcgaata ataacgtcta tgtgcgcggc cctgatcttg
      901 aaacggaatg gagcggtaag ctgaatgtca cgggcacagc caaggagctg gagattaaag
      961 gtggcctgtc tctggtgcgc ggcaggtttc agcttcttga tacgcctgtg cagctttcaa
     1021 agggcaatgt acgttttgcc ggggatgatc cggctaaccc tgacatagat gtatcgggta
     1081 cgatcaaggg gcgcgatatg gacgccattc tcaaaataac aggtgagacc aaagcccctg
     1141 aaatcggtct ccaatccgaa ccgcctctgc cggaagatga aattctggcc aaaaccctgt
     1201 tcggcaagtc ggtcagtcag ctttcgcctg tgcaagcctt acgcatcgcg cagcttatgg
     1261 cttatctctc cggacggcag gaagcatctt ttgatccgct gaatgatatt cgccgcgcta
     1321 ttggtataga tacgctcagt gtcggcatgg atgatgagaa aggtgcaaca ctatctatcg
     1381 gcaaatacgt caacgacagg gtctatattg gggtcgatca gggcacgaca ccgggcagtt
     1441 cagctgtacg ggcagaaatt gaagtcacag acgatattga agtggaaacc aagacaaatt
     1501 ctaccaacga gagtagcgtg ggtgtaaact ggaagaaaga ttattaggga tatgtgggtg
     1561 cgtgccgaaa acgtttttgc ggcaattgtc gaaacgcgtt atttttttgc cgcgcagaaa
     1621 atcatcgtcg agcgcaggct cacctgcttc taccaccacc ccgtaatgtc tgaaaattcc
     1681 atataatgga acatagacca catcacctac ttgaaagtta tattgattta tcatttgacg
     1741 tttggcctta cctgttgtat tttaaagtat atattcaaaa tacgcataaa tgagaaataa
     1801 tgcaaccaga aacttcatat cctgcgataa taggccgcat tcttgagcac cataggaaag
     1861 aagctggcta tgatcagggg gatattgcag aggctttagg gcttacgcaa tctgcttggt
     1921 cacgaattga gcgaggacaa tcaggtctat cgttagaata tcttgttagg ttcagcgaaa
     1981 taataaaaac cgagccccat aaaattgttg aagatgcgga ttatgcttcc aaaagaatta
     2041 aagctgatgg ggttatggtt cacccgcatg taatcacgaa taccgataag gtcgttgcgg
     2101 ctttaagctt agctgcgttg ggtatgatga tttttgcgat attgaaaaaa tgagggcata
     2161 aaaatgaaag ctgtaatact cgcaagaata tcaagcaaag agcaacggga tggacattcg
     2221 cttgatgccc agattaaaaa cctgaagcta cccctatcta ttccgagggt tgattaaatg
     2281 cgctgtatca ggccgacaag tcacctgtga ccttaaaaag ggcaaatcgt ctatttgatt
     2341 gttagagacc cagagaacca taaaaagaag ctgtggatca aagagcgcga tgttctggag
     2401 cagatagaag ctgtctttgc ctctattcag gttcccaaag cttatctatt tgcggggtta
     2461 accgggaatt gcatgcttaa gcgttttttg tcgctgatgc cctataatcg gtagttttta
     2521 acctcggcca cggtaattcg gtacatcctg ggcgggaatc cagatatctt ttggctcttc
     2581 accggtctga tagaaaatat caatcgggat gcctccgcgt gggtaccaat aaccaccgag
     2641 gcgcagccat tttgcttgtg ttgtttcctg gaggcgtttg gcaattgaga tcgtgcaggc
     2701 ctcatggaaa tcaccgtgat tgcggtaaga tgttaggaac agtttgaggg atttgctctc
     2761 gaccagccag tcgcctggca cgtaatcgag cacgagatgg gcaaagtcgg gctgaccggt
     2821 gagtgggcag atcgatgtga attcgggcgc tgtgaaacgc acggcatagc caacgtcttt
     2881 gtgcgggttg ggaacgcgct caagcactgc tttatcgggg ctgacgggga ttttgctttc
     2941 accaccgagt tgagtgaggt tttggtaaat agttttatcg gtcatggttt aaactcctcg
     3001 tttattgccc cagagcatga catgcagttg gggcaggacg tgcgcgttgg tattatcatt
     3061 cgtgactttt tcagccagcc attcaaggcg ggcgttcagg cctggcaggt cggcggggcc
     3121 ttcggttttg tgatttccgg gttgaaggta aagcggtaaa tcgggatagt gtgcggcagc
     3181 ctctttagcg taggcgtaat caatttcatc aaaaatgact attttgagag cgatttgcgg
     3241 tttattgctg gcagctttca ggcattcatc aaacttgtcc cagttggtgt catggccgct
     3301 ggaggggggt ttgggggata gggtcaggac gtcgagatcg gtgaaccagt tctgggcgat
     3361 tgttccttgg gtttccatgg caaagcggta gccttgttca tggccgagag tgatgagtgg
     3421 gtcgagtggc tggatggccg ggttaccacc agagagcgtg accatcatag gctggtcttc
     3481 gctcagacgt gtaatttcat tcatgatctc tttggcgttc atgggcttcc agtcatggcg
     3541 ataaacgctg tcaactgcgt gaagcgtgtc gcaccatgtg cagcggtaat cgcaaccgcc
     3601 ggtgcgcaca aagacggttg gctgaccaat aagcgcgcct tcgccctgga ttgtcgggcc
     3661 gaaaatttcg cttattctaa tgttttcgtt cattgttctt actctgttat tgcgaggagg
     3721 ctgaaagccg atgcggcaat ccatctacgg agttggattg cttcgctggc gctcgcaatg
     3781 acggttaaat agtttttcat gagcggtatt ccgcgctggt ttttggggtt tcctttacgc
     3841 gcacggcgga gacttccggc catttatctt tgcaccagtc atagaaatgt ttagcgatcc
     3901 tttcggcggt ggtgttgttt tcactcagca cgtcgttgag gtggcggtgg tcgatttctt
     3961 catcgatgta ttttttgagt gcatcaagtt cgcggtaatc atggacaaat ccggtggaat
     4021 tcaacttctc actttgtaat tcgacttcga caacatagtt gtgtccgtgc aaacgtgcgc
     4081 aagggtgatc atctgcaaga ccgtgtaaaa tatgagaggc ggagaagtgg tattcttttg
     4141 atattgtgta catcaggcgc cactttcttt tttgatgcga cacgtttcga tccaaaaatc
     4201 cgggtcttca tatcctgtag ggtcattaat gcccgcgatg ttaaaagcct cctggcgctc
     4261 aacgcaagtt ccgcagcggc cacagtgaat ttttccgcct ttataacaag accatgtttc
     4321 gataaacgga acgccgagcc gtgtaccttc ggcgacaata tcggctttgg ttttgtcaag
     4381 aaaaggtgtg taaagttcaa tgtttgagac tcctgcaaga gcttcatttt gcatggcggc
     4441 gaaggctttg ataaagctgg ggcggcaatc aggatagatg aaatgatcgc cgccatgcac
     4501 ggcggtggca acggcgtcat ccttgtttgc cgaggcgatg ccataggcga tggtgagcat
     4561 gatggcattg cggttgggga caatggtgat cttcattgag ttttccgcat aatgtccgtc
     4621 aggaacggta acatcgtcgg tcagggctga tccggtaagc gcagcgccga cagcggaaat
     4681 gtcgatgatg tgatgaggca cacccagagc acgcgcgtga tgcgcggcgc atttcagttc
     4741 ttttttatgg cgctggccgt aatcgaacga aattaaacgg gtgagggtac gctgtttagc
     4801 cgttttataa gccaaagtga tggaatcgag cccgccggag cagattacgg ctgttttcat
     4861 attattaagt ccttgttttg tttaaccggg taggaaatct tcgaccggtg ggcggcttat
     4921 agcacttcaa tctggaaaag ggaagagaaa gcctggaaat tttgttgatg cttattgata
     4981 aatttctata aagctttaaa gtcctttttt atgaatgtag ttgttgatag tgaaaaacgt
     5041 agaagcgttt tttctttttt aaaaaaacct ggcaagcagg ttttttggct ttggatcgtc
     5101 tatcagacta tcaaggggac gttgacgctg tctttgatct ggattccttt attctggctt
     5161 tggatgaatt cctaaatcta ttctatacgt gcaggctgat aatctttcag gcagacaaag
     5221 ccgttttgtg cggcaacaag cgtttcgcgg ctgaggcgtg cggcaatagt gccgggcggc
     5281 aatgcgtgat cttctttcca gacatccaga ttgtaaacca cccagagctg ctcatcaaag
     5341 ctggcaagac tgccgaagtt accgaaggcg cgggcggtgc gcgcgatgtt ttcaatactt
     5401 tcgttccagt tcagaaggcg atcatgatcg ctagggggct ttattgtggt tgctttgttt
     5461 tcatcctgta cagaggcgtt attccacaat tcaggtaggt ttgtgaaaag attacttacc
     5521 agttcaggtg cgcgcagggc gattttggcg ctgtaggttt cgacgctttc atttggggaa
     5581 agattgacct tttcttgtaa gagaatatca ccggcatcaa agtcttgcgt cattttatgg
     5641 gcggtcagtc cggcggcatc gatcatgttt tccaggatga tgcgcgggat gggcataagg
     5701 ccacgggctt tgggcaaata tgttggatgc aggtttacag cgtaggcgcg ggccggatcg
     5761 atgggcggga ttttgtaggg gtagccagcg gcaaagaaaa gctcgacgcc tttatcaaga
     5821 aagggattga ggtggaaatc ttcggctttt gagataatga acggaacgtt tcgtttctga
     5881 gcgagggcct ggcattgccg attaaaattg aaaatattgt cgcattcgaa gctgaaaata
     5941 ccggtgagtt cgtgaccttc atccaaggct ctttggatgg caggcagcat aaaatcataa
     6001 ccgaaataca caaatttcat gctatgagta ttgatgatgc ccggggatgt tacaagcttg
     6061 atttacaggt cttgacccta ggctttgtca tcaaagaggc ggaagagttc ttcactgagc
     6121 tcggaggcgg cttcaatggt tttgatgttg gctttgtatt gaatctttgc cagattgaga
     6181 ttgactgctt cttcggccag atcaacatta ggcataccga tgaggccttc gttattggca
     6241 aatggtgaat ccggggcata ggccgggacg aagggatcgt ttttgggtac gaatgtgctg
     6301 gtcacccctt ggccattgcc ttcattatct gtttgcgcgg tttgctgcgc gcgcagaggt
     6361 gtatagggca tttgctcgcc ttcttccagc gagccggtgg tttgcaaatt ggcaatattg
     6421 gacgcgctgg cggtgagttg tttattcgcc gccgctaaac cggaaagtgc aatgttaatt
     6481 ggtccaatca tatctagtat tatagcatga aaaacgttaa aatcgtcttg aaatccagca
     6541 tttttgctgt tttcctgccg ttttatatgc ctcttaatat gaaggatgaa ttatgcgttt
     6601 tataaaggtt tttcaacttg ttatgttttt tttgcttggc gtatcggtta atgccgggat
     6661 gggctttgca cgtgattcta tttggggggc gccgcaagat tatactgtgc gtccgttctt
     6721 ggaagaagct aaattgccgc ataattcacg ctgggctgat gatgaatgga gtccgcagga
     6781 ttggattgat tcgcgtggcg gtagtgttgt cgcggtcgtt gatggttttt atagggccga
     6841 tattgtggtt gatcaatatg ttgatgatga tgtgcctgta cttgaagttg ggcaggggtt
     6901 tctggacctg ggcgcgcagg accagcgccg cgttgttgcc tttatggatg aggtgtttgg
     6961 tgttacaaaa tttgcaacgc cgggtattat tcttgttctt tttcatcgtc aggataaaag
     7021 ctggcttcat ggcttgatag aaggcgatac tgagcggatt ggggtttttg atgctaatgg
     7081 tttgcagtta cagtaagaat tcctaattta taggcacgta aaaaccaagg ggcttgcccc
     7141 gtggatggag tgcgtccagt
//
LOCUS       DAOSWR010000106         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_6970_length_7027_cov_5.122347, whole genome shotgun sequence.
ACCESSION   DAOSWR010000106 DAOSWR010000000
VERSION     DAOSWR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_7027_cov_5.122347"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK513_07995"
     CDS             complement(<1..462)
                     /locus_tag="PK513_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO82427.1"
                     /translation="MFELEKFWISEAADHQAALEKTFATLKNEFEQTLNTCIQSIESG
                     GKILFFGNGGSAADAQHLATELTVRYINNRAPIPAIALTTDTSALTAIGNDYGFDHLF
                     SRQVEALGDPGDVAIAISTSGNSENILRGLKMAREKGLKTIGLSGRDGGKMP"
     gene            complement(480..1226)
                     /locus_tag="PK513_08000"
     CDS             complement(480..1226)
                     /locus_tag="PK513_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82428.1"
                     /translation="MKPLRFFALGICFLGLSACQTFDGVVKDLESIKLPALKNSTATS
                     AQQLVYSGNCPSVEAVPELQSLSEFTEITDQSDYNLISSVEITNMQSACNYDERAVTI
                     DLSMNFSGELGPQGKAPGSFSYPFFVAITSARGEILAKEIFTASLSYEPGQTTQTYTE
                     KLRQIIPIENKDHGARYKVLVGFQLTPDQLTFNRKAIADEIAQKEAEEKLLRQAQKMK
                     KSTFESGAQNAQEQAAKDNTYIGKPITITP"
     gene            1281..2270
                     /gene="ispH"
                     /locus_tag="PK513_08005"
                     /gene_synonym="lytB"
     CDS             1281..2270
                     /gene="ispH"
                     /locus_tag="PK513_08005"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOO82429.1"
                     /translation="MTQSKSMMDNKRKVTILLAAPRGFCAGVDRAIQIVEMALEKYGA
                     PVYVRHEIVHNKFVVDSLKAKGAVFVEELDEIPADAPGDQRPVIFSAHGVAKSVPEDA
                     QARAMFYVDATCPLVSKVHREAERYFEAGHQIILIGHKGHPEVIGTMGQLPDGAVLLV
                     ETRADVEALCVQAPENLAYCTQTTLSVEDTADIVAALRAKFPAICGPHKEDICYATTN
                     RQEAVKAIAPQSDAVIVIGAPNSSNSNRLVEVSSQHGCAKALLVQRASDIDWGWMEGV
                     QTLGITAGASAPDALVDEVIEAARARFDVSIREVAITQENVNFKLPKILEQAA"
     gene            2289..3092
                     /locus_tag="PK513_08010"
     CDS             2289..3092
                     /locus_tag="PK513_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOO82430.1"
                     /translation="MPITVSEFNDIERKVITLMGMSGVGKTYLSSMLARQGWYHYSCD
                     YEIGTRFLGDEIVQTLGVENTITRGDISQLSDYVGRLGNPDKGGMSLEEFRRRQNAYF
                     NAECAALARLPERVRLAHEQGIMHVVNDSTGSMCEIDDKILLDMVDENSLIVYIKASP
                     EEEKVVLERAKAYPKPLFYSPERFDRWLSKYEQEEGVEVVEDIDPDNFSRWAFPRLFE
                     NRLPKYQFIADKYGVTIPSEAFRGVESEAAFLDVIVEGLHKQEAMKRRA"
     gene            3089..3520
                     /gene="rnhA"
                     /locus_tag="PK513_08015"
     CDS             3089..3520
                     /gene="rnhA"
                     /locus_tag="PK513_08015"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOO82431.1"
                     /translation="MSKVVEIYTDGACSGNPGPGGWGAVMRWNGHEKELCGGEAETTN
                     NRMEMMAVIQALKAIKGHPKIEIYTDSKYVMQGITEWLDGWKARGWKTAGKKPVKNVD
                     LWQRMDELVSRHDVTFHWVKGHDGHPENERADSLAVAGIPQ"
     gene            4044..5741
                     /locus_tag="PK513_08020"
     CDS             4044..5741
                     /locus_tag="PK513_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO82432.1"
                     /translation="MIIEEQSIEETDNAHDKEVSTEQTAVIDNTSSQDAAMSENNSVV
                     NNIKNFSNLDFIKAAGTDQENGDGFDRPSSIELNSFGSTYLGTQTVYENFVSLGQIFK
                     TIPLNLRAQPATQLRLSSYNSILKNLKEVDVFEQPYQDGKISSQRPDTFENDLYQSSN
                     TIGNVIGLTNISHKESDGTNTSENPTDTGPAQVIIGIIDQGAYASQFSNNDYLYINEG
                     ETPGDGIDNDNNGYIDDYTGYSLVNNSTTDTEHAGPENNGWIPYPVMTHGVGMTNNVI
                     DQLNAAETITGTAPDAKIMTLKFTETNLGSYFGVTRAIEAGLENGARIFSLSFSVDSE
                     SYFNLWSDMLAPHDAVLVTYAGSSGVDSGEPESGEMHADNIIKVTRIQSDGSNFFSGD
                     GIEFVEESGAHSSSISIVAGKVAAIWAQDPDMSLTDVRFALEKSASQDVPILVNNGAD
                     TATQYGIIDLATAIDIVQNGKITLIDGNSFTNPEQIIGFNEVMDRIDISDVLDGYDLV
                     DDAIADFLQITDNGMDSTVYVDADGGGDNFQAVALILGVTGLTDEQALEASGNIITV"
     gene            complement(5758..>7027)
                     /locus_tag="PK513_08025"
     CDS             complement(5758..>7027)
                     /locus_tag="PK513_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO82433.1"
                     /translation="LNAMVDAGYITQTQAKGDKSIPPKPTQKPTDALADRYYTDWIVD
                     SLDELIGTPSEDLIIETTLNPEIQKYVQDAVSTTIEKNGSERSISQGAMIVMRPNGAV
                     VALIGGRNYSSSQFNRATQAKRPPGSSFKPLVYLTALEQGWDPQSLIMDKPITSGRYR
                     PKNFGNKYYGEVTLEEALTYSLNTVSYQLMKETGPEAVIYTAQRMGITSELEPDLSLA
                     LGSSGITLLEMATAYSTIANGGYAVEPFGITKIISKDTGELYYQRPPHRTMKRVFAAH
                     NIQNLTSMMNSVMEYGTGRAAQIGLPAAGKTGTSNDSRDALFMGFTRELVGVVWLGND
                     DNSPMKHVTGGSFPAQIWASVMRRAAGQYPQMNQSDFAPASGFEELLGSLFFRSERDK
                     ELHMQTLGNIQPATGEGRKRSLKESLRYND"
BASE COUNT         1633 a         1335 c         2129 g         1930 t
ORIGIN      
        1 gggcattttg ccgccgtcac ggccagacag gccgatggtt ttgaggcctt tttcgcgcgc
       61 cattttgagg ccgcgcagga tgttttcact gttgcccgag gtggagatgg cgatggcgac
      121 gtcgccgggg tctccgaggg cttcgacttg ccgggaaaag aggtgatcga agccgtaatc
      181 attgccgatg gcggtgagtg cggaggtgtc ggtggtcagg gctatggcgg gaatgggggc
      241 gcggttattg atgtagcgta cagtgagttc ggtggcaagg tgttgggcat ctgccgcgct
      301 gccgccattg ccaaagaaca gtattttccc tccgctttca atagactgga tgcaagtgtt
      361 tagtgtttgc tcgaattcat ttttgagtgt ggcgaaggtt ttttctaagg cggcttggtg
      421 gtccgcggct tcgctgatcc agaatttttc aagttcaaac ataagcaagg cctgtttttt
      481 tatggtgtga tggttatggg tttgccaata taggtattgt cttttgctgc ttgttcctgt
      541 gcgttttggg ctccagattc aaaggtactc tttttcattt tttgggcctg gcgcaggagc
      601 ttttcttcag cttctttctg agcgatttca tcggcgattg ctttgcggtt gaaggtcagt
      661 tggtccggtg tgagctggaa gccaacgagg actttatagc gtgcgccatg gtctttgttt
      721 tcgatcggga tgatttggcg cagtttttcg gtgtaggttt gcgtggtttg gccaggctca
      781 tagcttaaag atgcggtgaa aatttctttg gcgagaattt cgcctctggc cgatgtgatg
      841 gcgacgaaaa acgggtagga gaaagagccc ggcgcttttc cctgcggtcc gagctcgccg
      901 gaaaagttca tgctcaggtc gatggtgacg gcgcgttcat catagttgca agcgctttgc
      961 atgttggtga tctcgacgct ggagatgagg ttataatcgc tttggtccgt gatttcggtg
     1021 aattcggaga gggactggag ttcaggaacg gcttccacag atgggcaatt gccgctgtag
     1081 acgagttgtt gtgcgctggt ggcggttgag tttttaaggg cggggagttt tatggattca
     1141 aggtctttga cgactccatc gaatgtttgg caggcggaaa gtcccaaaaa acaaatacca
     1201 agggcaaaaa atcttaaagg tttcatttgc tgtatcccga tgctagaaag cttgaatgtc
     1261 taagcagctt acgagagcgt atgacgcaaa gcaagagtat gatggacaat aaacgtaaag
     1321 taacgatttt actggcggcg ccgcgggggt tttgtgcagg cgtggatcgg gctattcaga
     1381 ttgttgagat ggcgctggag aaatatggcg cgccggttta tgtgcggcat gaaattgtgc
     1441 ataataaatt tgttgtcgat agtttgaagg ccaagggggc ggtgtttgtt gaggaactgg
     1501 acgaaattcc ggcggatgcg ccgggggatc agcggccagt gattttttcg gcgcacgggg
     1561 tggcgaagtc tgtgccggag gatgcgcagg cgcgagctat gttttatgtg gatgcgacat
     1621 gtcctttggt gagtaaggtt catcgggagg cggagcggta ttttgaggct gggcatcaga
     1681 ttattcttat tgggcataag ggacatccag aggtgattgg gacgatgggg caattgcctg
     1741 atggcgctgt tttgctggtt gaaacgcggg cggatgttga ggcactatgt gttcaggctc
     1801 ctgaaaatct ggcgtattgt acgcaaacga cgttgtcggt ggaggatacg gcggatattg
     1861 tggcggcgct tcgggcaaaa tttccggcta tttgcgggcc gcataaggaa gatatttgtt
     1921 atgcaacgac gaacaggcag gaagcggtga aggctatcgc gccgcaaagc gatgcggtga
     1981 ttgttatcgg cgcgccgaat tcgtcgaatt ccaaccggct ggtggaggtt tcgagtcagc
     2041 atggttgtgc gaaggcgttg ttggtgcagc gggcgagcga tattgactgg ggctggatgg
     2101 agggtgtgca aacgttgggg attacggccg gggcgagtgc gccggatgcg ttagtggatg
     2161 aagtgattga agcggcgcgg gcgcgttttg atgtttctat tcgtgaagtg gcgattacgc
     2221 aagagaatgt gaattttaag ctccctaaaa ttttagagca ggccgcataa acaaggaaag
     2281 agagccccat gcctattacg gtttctgaat ttaacgatat tgagcgcaag gtgattacgc
     2341 tgatgggaat gtccggcgtt gggaagacat atctttcgag tatgctggcg aggcagggct
     2401 ggtatcatta ttcgtgtgat tatgagattg gaacgcggtt tttgggcgat gaaattgtgc
     2461 agacactggg cgttgaaaat accattacac gcggtgatat ttcgcagctt tcagattatg
     2521 tcggacggtt ggggaaccct gataaagggg gaatgtctct ggaggagttt cggcgacggc
     2581 agaatgcgta ttttaatgcg gagtgcgcgg cattggcaag gttgcctgag cgtgtgaggt
     2641 tggcgcatga gcaaggtatt atgcatgttg tgaatgattc cacaggttct atgtgtgaga
     2701 ttgatgacaa gattttgctg gatatggtcg atgagaattc attgattgtt tatatcaagg
     2761 ctagccctga agaagaaaag gtggttttag agcgtgcaaa agcttatccc aagccgttgt
     2821 tttattcgcc ggagcggttt gatcgctggt taagtaagta tgagcaagag gaaggcgttg
     2881 aagtggtaga ggatattgat cctgataact tttcgcgctg ggcgtttccg cggctttttg
     2941 aaaaccgttt gccgaaatat cagtttattg cagataaata cggggtgacg attccttcgg
     3001 aggcgtttcg cggtgtggag agtgaggcgg cgtttttgga cgtgattgtt gaagggttgc
     3061 ataagcaaga ggctatgaag cggcgcgcat gagcaaggtt gttgaaatct atactgatgg
     3121 agcgtgttcc ggcaatcctg gtcctggggg ctggggggcg gtgatgcgct ggaacggaca
     3181 tgagaaggaa ttgtgcgggg gcgaagcgga gacgacgaat aaccgtatgg aaatgatggc
     3241 ggtgattcag gctttgaaag cgattaaggg gcatccaaag attgagattt ataccgacag
     3301 caaatatgtg atgcagggga ttaccgaatg gctggatggg tggaaggcca gaggatggaa
     3361 aacagcggga aagaagccgg tcaagaatgt tgatttatgg cagcggatgg acgaactggt
     3421 ttcccggcat gatgttacgt ttcactgggt gaaggggcat gacgggcatc cggagaatga
     3481 gcgggcagat tcgctggcag ttgcggggat accgcaatga ccaacttcaa tctctttcag
     3541 gtttaattcc tcgccgctta cggcgtaatc gtcatcgcga gcgaagcgat ccagtgccac
     3601 aaggataaat tctggattgc ttcaccacct ttggcggttc gcaatgacgt ggtacctcgc
     3661 cccttggggc gggggtgttc atttgcaggt atttgctttc tttttaggct tgttcacttg
     3721 gctttgtgcg ggtttggggc gttttagctg tgtattaatg aagtgttaag attcatcggc
     3781 agatgatggt tgcagagcca tttaagccat agccgggaag gtttaatgtt gatccaatca
     3841 ctccataatg tgggttaaaa taattaattt gctataaata ttaataaaat tttacataaa
     3901 ttcagtttaa tatattagaa gggaagggga tatattatta cgtttttagg aaaagcaaaa
     3961 taataacaat gttttatata tcctttcata tgttaaggcg tttgttttat atgcatagta
     4021 aataagctgg gttagtgatt tttatgatta ttgaagaaca aagtatcgaa gaaactgata
     4081 acgcacatga taaagaggtt tctactgaac aaactgctgt gattgataat accagttctc
     4141 aagatgcggc aatgtccgag aacaatagcg ttgtcaataa tattaaaaat tttagcaacc
     4201 tggatttcat aaaagctgcg ggcacagatc aggaaaatgg cgatggtttc gatagacctt
     4261 cgtctataga acttaatagt tttggttcta cttatctggg cacacagaca gtttatgaaa
     4321 attttgtatc gctggggcaa attttcaaaa ccattcctct caatttaaga gcacagcctg
     4381 cgacacaact gcggctttcg agctacaaca gcattctgaa aaacctgaaa gaggtagatg
     4441 ttttcgaaca gccatatcaa gatggaaaaa tatcaagcca gcgccccgac acttttgaaa
     4501 acgacctata ccaatcgtcg aacacaattg gcaatgttat aggactgaca aacatctctc
     4561 ataaagaaag tgacggaacc aacacatcag aaaacccgac tgataccgga cctgcacaag
     4621 ttatcatagg cattattgat cagggcgcgt atgccagcca attttctaac aatgattatt
     4681 tgtatatcaa tgaaggggaa acaccgggcg acggtattga caatgacaat aacggctata
     4741 ttgatgatta tactggctat agcttggtaa acaatagcac aacggatacc gagcatgcgg
     4801 gtcctgagaa taatggctgg attccctatc ctgtaatgac gcatggtgta ggcatgacga
     4861 ataacgtgat tgatcagttg aatgcggcgg aaacgattac aggaacggcg ccagacgcta
     4921 aaatcatgac tcttaaattt acagaaacaa atttgggttc atattttggt gtaacgcggg
     4981 ctatagaagc cgggctggaa aacggtgctc gtatcttcag cttgagcttc agtgttgatt
     5041 cagagtctta ttttaatctg tggtcggaca tgctcgcacc tcatgatgcc gttcttgtca
     5101 cgtatgcggg ttcttccggt gttgattccg gagagccgga atccggggaa atgcatgcag
     5161 ataatatcat aaaggttacc cgcattcaga gcgatgggag taacttcttt tctggagacg
     5221 gtatcgaatt cgtcgaggag agcggcgcgc attcctcctc tatctcgata gttgccggca
     5281 aggtcgccgc tatatgggcg caggacccgg atatgagcct caccgatgtt cgctttgcgc
     5341 ttgaaaaatc agcctctcag gatgtgccga ttctggttaa taacggcgca gatacggcca
     5401 cgcagtatgg cataatcgat cttgcgacgg ccattgatat cgtgcagaac ggaaagataa
     5461 ccctcattga tggcaattcc ttcacaaacc ctgagcagat tatcggtttt aatgaagtca
     5521 tggatcggat agacatttca gatgtccttg acggctatga tctggttgac gatgcaattg
     5581 cagactttct gcaaattact gataatggca tggattcaac ggtctatgtt gatgctgatg
     5641 gcggtggtga taatttccag gcggtcgcgc ttatcttggg cgtcacaggt ctgactgacg
     5701 agcaagctct tgaagcttca ggtaatatta ttacggttta ggattcagat tcacagatta
     5761 atcgttatag cggagggatt ctttgagtga gcgttttctg ccttcgccgg tggctggctg
     5821 gatatttccc aaagtttgca tgtggagttc tttgtctctt tccgagcgaa agaagaggct
     5881 gccaaggagt tcttcgaagc cggaagccgg ggcgaagtcg ctctggttca tttgcgggta
     5941 ttgaccggcg gcgcggcgca tgacgctggc ccagatttgt gccgggaagc tgccgcctgt
     6001 gacgtgtttc atgggggagt tatcatcgtt gccaagccag acgacgccga cgagttcgcg
     6061 tgtgaagccc atgaacaggg catcgcggct atcgttgctg gtgccggttt ttccagcagc
     6121 aggaaggccg atttgggcgg cgcggcctgt gccgtattcc atgacgctgt tcatcataga
     6181 ggtgaggttc tggatgttat gggcggcgaa tacgcgtttc attgtgcgat ggggcgggcg
     6241 ttgataataa agctcgccgg tgtctttgga tatgattttt gtgatgccga agggttcaac
     6301 tgcgtagcct ccattggcaa tggtggagta tgcggtggcc atttccagta gtgtaatgcc
     6361 gctggaaccg agggccagag aaaggtccgg ttcaagttct gaggttattc ccatgcgctg
     6421 cgcggtgtag atgacggctt cagggccggt ttccttcatg agttggtagg acacggtgtt
     6481 cagggagtaa gtcagggctt cttcaagcgt aacttcgccg taatatttgt tgccgaaatt
     6541 cttcgggcgg tagcgtccac tggtgatggg tttatccatg atcagacttt gcgggtccca
     6601 gccttgttcg agggcggtga ggtagactag cggtttaaac gaggagccag gcggtcgctt
     6661 ggcttgtgtg gcgcggttga actggcttga tgagtaattg cgtccgccga tgagggcgac
     6721 aacggcgcca ttggggcgca tgacgatcat ggcgccttgg gaaatagagc gctcgcttcc
     6781 atttttttct atggttgtgg agacggcgtc ttgtacgtat ttctggattt cagggtttag
     6841 tgtggtttcg atgatcaaat cttcgctcgg tgtgccgatg agctcgtcga gtgaatcgac
     6901 gatccagtct gtgtagtagc ggtcggccaa agcgtctgtg ggtttttgtg tcggtttggg
     6961 agggatggac ttatcgcctt tggcttgtgt ttgtgtgatg tagcccgcgt cgaccatagc
     7021 atttaga
//
LOCUS       DAOSWR010000107         6922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7095_length_6922_cov_6.083297, whole genome shotgun sequence.
ACCESSION   DAOSWR010000107 DAOSWR010000000
VERSION     DAOSWR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7095_length_6922_cov_6.083297"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..464)
                     /locus_tag="PK513_08030"
     CDS             complement(189..464)
                     /locus_tag="PK513_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82434.1"
                     /translation="MPEPALKDQKPGAVRVLLVEDDPVTRWMVRSALKKECEFVTAQS
                     ANNAFGLYASYQPDVVFLDIDLPDGNGHDVLKWIMRNDPGACVVTHG"
     gene            496..978
                     /locus_tag="PK513_08035"
     CDS             496..978
                     /locus_tag="PK513_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO82435.1"
                     /translation="MFFRLNVLQIALPPLRERKQDIPVLAGHFIERFCVNEGAIPKDI
                     SEATEKQLINYDWPGNVRQLENVINRAMVVSDGNVLNVENLSGLVSETETPSASSKTS
                     RNIRIISDDGDFKSINEIEQEAMQIALDHFDHNITQAAKVLGMAKSTFYKKMKNVQNN
                     "
     gene            1541..1921
                     /locus_tag="PK513_08040"
     CDS             1541..1921
                     /locus_tag="PK513_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82436.1"
                     /translation="MSDNHPLDGQYRITSTTSYKGPLEKRSDGETRIKNSQTQRTDDA
                     GCKWTSTFEILNDNEVKMISLADPSEAAIDFLLTAPDGSPSRNPVTYKTTLKLARKGD
                     KIQMSGQIEYGHDVVFLTMRKIGD"
     gene            complement(1918..2823)
                     /locus_tag="PK513_08045"
     CDS             complement(1918..2823)
                     /locus_tag="PK513_08045"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOO82437.1"
                     /translation="MDAHSFSQSSNHLLMIEPADFYANPETMETNAYQVDDHEPVEVT
                     SARALREFRELHDKLVESGVKITTAKALPQCPDMVFPNWFFSFPDGRLMLCPMLNKNR
                     QDERSEDIIALLTKIYPDVIDWTEYEEFGLALESTASIVSDRVNRRAYAALSARTDEG
                     LARKWADHIGYEIDFFETHSHTGIPVYHTDCVMWIGSTLAGICSECIVEEDRRRIVSS
                     LLDTHQVIEFSNDQLRTFCGNALEVVGHGGQKMLVLSEKALQALEEDQLEKCHQHFSR
                     LIYSPLPTLERYGGGSARCMLAELL"
     gene            complement(2956..3267)
                     /locus_tag="PK513_08050"
     CDS             complement(2956..3267)
                     /locus_tag="PK513_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82438.1"
                     /translation="MDHVHATALLKNPFSSKNWEKNIRQVSVEPCDVHEDPLGYRAVF
                     FLREDDERAETCEMFVVGASYLAQREYNLTKAGYVAPMTQKAIGLIEGKIGTRLPVEL
                     A"
     gene            complement(3398..4048)
                     /locus_tag="PK513_08055"
     CDS             complement(3398..4048)
                     /locus_tag="PK513_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO82439.1"
                     /translation="MSDRISSETPEATSSSYEVRQERRLTRDQDAYFNAQFDAVALYV
                     EPGSANCTVRKDEMLVATVGSGVLLAIYDPELKVGAMGYVMLPDAVLEHFPFLEQADR
                     DLVLKAFEPIEKCIGELKRRGAAKNRIRIRLFGGSSEGDDPEERGLKNTVFVQEYIFR
                     KGLQVLNADIGGPFIRRVHFFPATGRAVRRLLKRKDDFDNLHLAESDFNKKITIDT"
     gene            complement(4101..5075)
                     /locus_tag="PK513_08060"
     CDS             complement(4101..5075)
                     /locus_tag="PK513_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOO82440.1"
                     /translation="MVDGWLEAPYDLSGKRVWVAGHGGMVGAALVVRLRSEDCEILTV
                     SREGLDLRDQAATRAWIVQNKPDVVIIAAAKVGGILANSTYPADFLYDNMMIEANIIH
                     AAHEAGVEKLLFLGSSCIYPKEAAQPIGEDALLTGALEPTNEAYAVAKIAGIKLCQAY
                     REQYGRDFISAMPCNLYGPGDTFDEQGSHVIPALMMKAHAAKEIGADIFEVWGSGTPL
                     REFLYVDDLADGLVFLLKNYSGTMPVNIGVGEDISIRELAVIISETVGFTGRIAFDSS
                     KPDGAHRKLMDSSCIRNAGWTPKTTFQEGLSRTYQWYCEENKGIKKRA"
     gene            complement(5057..6124)
                     /gene="gmd"
                     /locus_tag="PK513_08065"
     CDS             complement(5057..6124)
                     /gene="gmd"
                     /locus_tag="PK513_08065"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO82441.1"
                     /translation="MTKTAFITGIAGQDGAYLAQFLREKGYSVHGLVRWDSYSDPIEG
                     LSRLDVLGLVSDDVSLHMGDVTDANNLNLLIREIRPDEIYNLAGLSQVQVSFETPAST
                     LQINTAGTLNILDAVRVNGLENYTRVYQASSSEMFGKARAPQNEDTKMEPCSPYGVAK
                     LAAYHLGKSYRESYGMYVANGILFNHESPLRGEDFVTRKITKAVAAVEAGSNDVLRLG
                     NLDSIRDWGDARDFVAGMWAILQRGAPDDYVLATGEAHSVREFAQAAFAHVGISLEWR
                     GAGVDEKGVEVKSGRVLIEVDRQLFRPSEVHHLLGDAAKARDVLGWTPKTTFEGLVAA
                     MVNADRAALHLDGGAAWSMAG"
     gene            complement(6209..>6922)
                     /locus_tag="PK513_08070"
     CDS             complement(6209..>6922)
                     /locus_tag="PK513_08070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOO82442.1"
                     /translation="EAGPAYGIWPRNWPLWTQVFLGMTTAEFGLYWAHRLAHEISWMW
                     RFHAVHHSVTKLWFLNTGRFHFVDSLISIVLGLWILLALGAPMEVVKWLSAITAFIGM
                     LTHCNVEMRFGLLNYIFNTPGLHRWHHSRLLYEGNRNYGENLMIWDQIFGTFYNHSYR
                     PSADIGMGDYMPEKFTYQILWPFLTNKMKQRLQTDFEPVPFGREGDADHPVPEFAKRK
                     SLFMLIVGLFQARNPRASV"
BASE COUNT         2012 a         1957 c         1381 g         1572 t
ORIGIN      
        1 cggtcataga tcgtagctta actcactgtc cgaattcttg ggaccacctc tctacttcgt
       61 aagcagcgta ggcgttgatg agcacgttat tcgccgttat gtagaacgtc aaggacaccg
      121 ggattcaggt caattccgcg aggaattgta atgcgcaaag cgcggcgcag ccccatagaa
      181 ccacgggttt acccgtgggt aaccacgcat gcgccgggat cgttacgcat aatccatttg
      241 agaacatcgt ggccattgcc gtctggcaga tcgatatcca gaaacaccac gtcgggctgg
      301 taactggcat aaagaccgaa ggcgttattg gcgctctggg cggttacaaa ttcgcattct
      361 tttttaagcg cgctgcgcac catccagcgt gtcactggat catcttccac cagcagaacc
      421 ctgacggcac ccggtttttg atctttcaag gccggttcgg gcaagggaag cgcctctaga
      481 aacttcaggg aggatttgtt tttccgcctg aatgtcctgc aaattgcgct gcccccctta
      541 cgggaacgca agcaggatat accggttctg gccgggcatt ttattgagcg cttttgcgtc
      601 aatgaaggcg ctattcccaa agacatatcg gaggctacag aaaaacaact tataaattat
      661 gactggcccg gcaatgtccg acagcttgaa aacgtgatta accgcgccat ggtcgtgagc
      721 gatggaaacg tgcttaatgt tgaaaatttg agcggactgg tatcggaaac ggaaacgcca
      781 tctgcaagca gcaaaacctc cagaaatatc cgcatcatct cggatgatgg cgattttaaa
      841 agcatcaatg aaatagaaca ggaagccatg cagattgctc ttgatcattt cgatcacaac
      901 attactcaag ccgccaaagt ccttggtatg gccaaatcca cgttttacaa gaaaatgaaa
      961 aatgtccaga acaattaggc tcagacccta ttgatgatgg attcacaaga taaaggattt
     1021 atgagattca cttgcggtga aagaagattt tatcatgagt gaattgtttt ggttatcgga
     1081 agagcagcta ttctggcgaa gcaacaacgc cagaatgaaa agcgcgaaga aaaaagaagt
     1141 ctgggggaca agcccccaga cttcaggcgt gtgtggggtt cctagttact ctgcccacca
     1201 gtgcggaact aaacactggc ttcgattatc acgcatcgcg gcacattggg ggaggattga
     1261 cccgaagtaa aaggccaaaa gcaccgctca agcgcgtgat aatactgtgg atggccaact
     1321 tttgcgaaat ttcacaaacc ggccataagc ttattaactt tatgctcacc aacatacagc
     1381 cacacacgct ttcaggtgaa taatttatat aattaaatac atttattatg taattgaggc
     1441 accttgtagc aagcgttatg aataatgtca aacaaaaaat cacaccctat tccaaagaac
     1501 acacctataa gctaagctag aactccaagg agaaaattca atgtctgaca atcacccact
     1561 ggatggccag tacagaatca ccagcacaac cagctataaa ggcccgctgg aaaagcgcag
     1621 cgacggcgaa acgcgtatta aaaacagcca aacacaacgc accgatgatg ctgggtgcaa
     1681 atggaccagc acatttgaga ttttaaatga caacgaagta aaaatgatat ccttggccga
     1741 tccatccgaa gccgcaattg attttctgct caccgcccct gatggatcac catcgcgtaa
     1801 ccccgtcacc tataaaacta ccctcaaact ggcgcgcaaa ggcgataaaa tccagatgtc
     1861 cggtcagatc gaatacggcc acgatgtcgt cttcctgacc atgcgcaaaa tcggggatta
     1921 gagaagctcc gccagcatac agcgcgcaga cccgccacca taacgctcta gcgtcggcag
     1981 tggactataa atcaacctgg aaaaatgttg atgacatttt tccagctggt cctcttcaag
     2041 cgcttgcaaa gctttttccg aaagcaccag catcttttgt cccccatgcc caacgacctc
     2101 cagcgcattc ccacaaaacg tacgtagttg atcattacta aactcaatca cctgatgtgt
     2161 gtcgagcaaa gacgacacaa tgcgcctccg gtcttcttca acaatacatt cgctacaaat
     2221 accagccagc gtgctgccaa tccacatcac acaatccgtg tgatacaccg gtatacctgt
     2281 gtggctatgc gtttcaaaga aatcgatttc atagcctata tgatctgccc atttacgtgc
     2341 caacccctca tcagttcgcg ccgacaacgc cgcataagcg cgtctgttca cccggtcaga
     2401 aacaatgctg gctgtacttt ccagcgccag accaaattct tcatattctg tccagtcaat
     2461 tacatccgga taaatctttg tcagcagggc aataatgtct tcactacgtt catcctgacg
     2521 gtttttattg agcattggac acaacatcaa acgcccatca ggaaatgaaa aaaaccagtt
     2581 cggaaacacc atgtccggac attgcggcaa cgccttggct gtcgtaattt tcacaccgct
     2641 ttccaccagc ttgtcatgca actcccgaaa ctcacgcaaa gcccgcgcgc tagtcacttc
     2701 aaccggctca tgatcatcaa cctgatacgc gttggtttcc atcgtctcag gattggcgta
     2761 aaaatcggca ggttcaatca tcaacaaatg attggaggat tgggaaaaag aatgtgcatc
     2821 catgatacaa accaaacttg cagggggaaa gaagcctaaa aggccttcag gcacaaacaa
     2881 gtatgcccga ttgggagata ttttacatcc ccaattagat aatttcttta gataaacgcc
     2941 aactttttga actaatcagg ccaattcgac cggtaaacgt gtaccgattt ttccttcaat
     3001 cagacctata gccttttgcg tcatcggcgc aacataaccg gccttggtca aattatactc
     3061 gcgctgcgcc agataagaag ccccgacaac aaacatttca catgtctctg cacgctcatc
     3121 atcttccctc aaaaagaaaa cagcacgata ccccaaggga tcctcatgca catcacaagg
     3181 ttcaacagag acctgacgaa tatttttctc ccagtttttg gaagaaaaag gattcttaag
     3241 caacgctgta gcatgaacat ggtccatagc caaaatcccc aaataaattc cttatactta
     3301 taagagatga ataatttatg caaaaaatga ggggaagtca agaaaaaact aaaaaaatca
     3361 ttatattaca atacactaaa caaacaaaag ttataactta tgtatcaatt gtgatttttt
     3421 tattaaaatc agattctgca agatgcaggt tatcaaagtc atcttttcgc ttcaacaagc
     3481 gccgaactgc acgccccgta gctggaaaaa aatgaacacg gcgaataaag ggcccgccaa
     3541 tatcagcatt caaaacctgc aaacctttac gaaaaatata ctcttgcaca aaaacagtat
     3601 ttttgagtcc gcgctcctcc ggatcatcgc cctcactgct tcctccaaaa agtcgaatgc
     3661 gtatgcggtt tttcgccgca ccccggcgct ttaattcacc gatgcacttc tcaatcggtt
     3721 caaaagcctt taaaacaagg tcccgatccg cttgctctaa aaaaggaaaa tgctcaagca
     3781 cggcatcggg caacataaca taccccatcg ccccaacttt aagctctggg tcataaatcg
     3841 ccaataacac tcctgaacca acagtggcaa ccagcatttc atccttgcgc accgtacaat
     3901 ttgcgctacc cggctcgaca tagagcgcta ccgcatcaaa ctgcgcattg aaataagcat
     3961 cctgatctcg tgttaaacga cgctcttgcc gcacttcata tgaagaagat gttgcttcag
     4021 gcgtttcaga tgaaattcga tcactcatgc aaacattata acgggaaagt taaaagaaag
     4081 tattaccaaa aatgcctgat tcaggctctt ttctttatac ctttattttc ctcgcaatac
     4141 cattgatagg ttcgagaaag cccctcctga aaagtagttt ttggcgtcca gcctgcattt
     4201 cgaatgcaag acgaatccat taatttgcga tgcgccccgt ccggcttact gctatcaaat
     4261 gcaatacgcc ccgtaaagcc aacagtttca gagattatca ctgccaactc acggatgcta
     4321 atatcctctc ccacaccaat attcaccggc atggtgccag aatagttttt gagcagaaac
     4381 acaagaccat cagccaaatc atcaacataa agaaactcgc gcaaaggcgt tccactcccc
     4441 cagacctcaa aaatatccgc tccgatctct ttagccgcat gcgccttcat catcaacgcc
     4501 ggaatgacat gcgaaccctg ttcatcaaac gtatcgcccg gcccataaag gttacacggc
     4561 atagcagaga taaaatcgcg cccatactgc tcgcgataag cctggcacaa ctttatccct
     4621 gcaattttag ccaccgcata cgcttcattg gtcggctcca gcgcacccgt aagcagcgca
     4681 tcctcaccaa tcggctgcgc cgcctctttt ggataaatac atgacgaacc caaaaacaac
     4741 aacttctcca cgcccgcctc atgggccgca tgaataatat tggcctcaat catcatatta
     4801 tcgtagagaa aatccgccgg ataagtgcta ttcgctaaaa tcccgccaac cttcgccgcc
     4861 gcaataatca caacatccgg tttattctga acaatccacg cccgcgttgc cgcctgatca
     4921 cgtaaatcaa gcccctcccg gctgacggtc aaaatctcac aatcctcaga tcgcaaccgc
     4981 accacgagcg cagccccaac catgccgcca tgcccagcca cccagacccg tttgccgctc
     5041 aaatcatacg gcgcctctaa ccagccatcg accatgccgc gcccccatca aggtgtaaag
     5101 ctgcgcggtc cgcattcacc atcgccgcaa ccagcccttc aaacgtcgtt ttaggagtcc
     5161 atcccaaaac gtcgcgcgcc ttggccgcat cgcccagtaa atgatgcact tcggaaggac
     5221 ggaataactg acgatcaacc tcaatcaaaa cccgcccgct tttcacctcc acgccttttt
     5281 catctacgcc tgcgccgcgc cattcaagcg atatacccac atgcgcaaaa gccgcctgag
     5341 caaactcacg tacgctatgc gcctcccctg tcgccaatac ataatcatcc ggagcgccgc
     5401 gctgtaaaat cgcccacatc ccggcaacaa aatcgcgtgc atcgccccaa tcccgaatgg
     5461 aatccagatt acccagccga agcacatcat tgctccccgc ctcaactgcc gcaaccgcct
     5521 tggtgatctt gcgagtaaca aaatcctccc cgcgcaacgg actttcatga ttaaacaaga
     5581 taccgttagc cacatacatg ccgtaggact cgcgatagct cttacccaaa tgatacgccg
     5641 ccagctttgc cacgccataa gggctgcacg gctccatttt cgtatcttca ttttgcggcg
     5701 cgcgcgcctt cccaaacatt tccgaagatg acgcctgata aacccgcgta taattctcca
     5761 gcccgtttac ccgtaccgca tccagaatat tgagcgtccc ggcagtatta atctgcaaag
     5821 tcgaggccgg cgtttcaaag ctcacctgaa cttgcgacag ccctgccaga ttataaatct
     5881 catccggacg aatttcgcga atcagcaaat taaggttatt cgcatccgtc acatctccca
     5941 tatgcaagct gacatcgtca ctcaccaacc ccaaaacatc aagccgcgaa agcccctcaa
     6001 tcggatcgct ataactgtcc cagcgcacca aaccatgcac actataccct ttctcgcgca
     6061 aaaattgcgc caaatatgcc ccgtcttgtc cggcaattcc tgtaataaag gctgtttttg
     6121 tcattcttac gtatggcctc aacgcataat tacgtatgca taaaaaatgc acgaaatcct
     6181 aagcttaagc aaagacttta tgaaagattt aaacgctggc gcgtggatta cgggcctgaa
     6241 acaatccgac gatcagcatg aacaatgatt tgcgctttgc aaattcaggc actgggtggt
     6301 ccgcatcacc ttcacggcca aacggcaccg gctcaaagtc cgtctgcaag cgctgcttca
     6361 tcttgtttgt cagaaaaggc cacaagatct gataggtgaa tttttccggc atataatccc
     6421 ccatcccgat atcggcagat ggccggtatg aatgattata aaacgttccg aaaatctgat
     6481 cccagatcat caaattctcg ccgtaatttc ggttcccctc atacaaaagc cggctatgat
     6541 gccagcgatg caggcctggc gtgttgaaaa tatagttcag cagcccgaac cgcatctcga
     6601 cattgcaatg ggtcagcatg ccaataaacg cggtaattgc gctcagccat tttactacct
     6661 ccatcggcgc gcccagcgcc agcaaaatcc agagccccaa cacaatactg atcagactgt
     6721 caacaaaatg gaaccggccg gtattcaaaa accacagctt ggtgacgctg tgatgaacgg
     6781 catgaaaccg ccacatccac gaaatttcat gcgccagacg gtgcgcccag tacaaaccga
     6841 attccgcagt tgtcatccct aaaaacactt gtgtccaaag cggccagttc cgcggccata
     6901 taccgtacgc tggcccggcc tc
//
LOCUS       DAOSWR010000108         6888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7142_length_6888_cov_5.149715, whole genome shotgun sequence.
ACCESSION   DAOSWR010000108 DAOSWR010000000
VERSION     DAOSWR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_6888_cov_5.149715"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PK513_08075"
     CDS             complement(<1..729)
                     /locus_tag="PK513_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82443.1"
                     /translation="MKKVPAALVAVVLSVSASAAFAQAAEGAQNNDILSLTPDAEPTT
                     NFDCSGKMAHEYAMNDYNQITAEDPALYSLSKRTLGFNGLEIKNLADRYGALRTALAE
                     KYETLFCGTVDRQNVSRKIYESIYKTGIFLRDVFELHGQMIAEEPEWFDRYYSDDYLK
                     MSIMHFLGVERPENAVDKQFLEGYAARRDELVPVYFAIDLATILGIEELPEMQAYLQT
                     LDRIMSEEYDKAFLGENQQDFLPLP"
     gene            complement(806..1225)
                     /gene="atpC"
                     /locus_tag="PK513_08080"
     CDS             complement(806..1225)
                     /gene="atpC"
                     /locus_tag="PK513_08080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOO82444.1"
                     /translation="MENNIQFELVSPEEKLVSQPVHMAVIPGDEGEFGVLAGHASLVA
                     SLRPGVVELYEQAGGEARKIFIAGGFADVSAEQCTVLAEEAVLVSDLDQTKIEQKIAD
                     LNEDLGIAEEAADKARIEKRLVLLKAKLSAVTGKLAA"
     gene            complement(1541..2995)
                     /gene="atpD"
                     /locus_tag="PK513_08085"
     CDS             complement(1541..2995)
                     /gene="atpD"
                     /locus_tag="PK513_08085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOO82445.1"
                     /translation="MAKAKAKSKATGAVGTILQVLGAVVDVQFNEGEVPAILNALETD
                     NQGNRLVLEVAQHLGENTVRAIAMDMTDGLVRGAEVVDTGAGISVPVGPEVLSRIMNV
                     TGEVIDEGPALKTKARWEIHRAAPAFEDQATEVEQLVTGIKVVDLLCPYAKGGKIGLF
                     GGAGVGKTVTIQELINNIAKGHGGVSVFGGVGERTREGNDLYHEMMDAGVIKEHDYKN
                     SKVALVFGQMNEPPGARARVALSALTMAEYFRDEEGQDVLFFLDNIFRFTQAGSEVSA
                     LLGRIPSAVGYQPTLSTEMGNMQERITSTNKGSITSIQAVYVPADDLTDPAPATTFAH
                     LDATTVLSRAISEMGIYPAVDPLDSTSRILDPRIIGQEHYDVANRVQQTLQQYKNLQD
                     IIAILGMDELSEEDKLVVARARKIQRFLSQPFHVAEVFTGTPGVFCQLEDTIKGFKAI
                     VDGQYDHLPESAFYMIGTIDQAVEKAKKMAAEAA"
     gene            complement(3022..3207)
                     /locus_tag="PK513_08090"
     CDS             complement(3022..3207)
                     /locus_tag="PK513_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82446.1"
                     /translation="MTDEVKVKVAVNRDWQEVQETAERRRDGWWVKSRRTASGKPVFP
                     SRESGTDSMNFTRAPGE"
     gene            complement(3232..4146)
                     /locus_tag="PK513_08095"
     CDS             complement(3232..4146)
                     /locus_tag="PK513_08095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOO82447.1"
                     /translation="MPSLRDYRDRIASVKSTRKITSAMKMVAASKLRKAQEQAEASQP
                     YAQCMADMMARVAGGVVVSDASSPLLAGTGSDQKHLLVIVTADRGLCGGFNGNLVKKA
                     RAKIKELQDMGKDVSIVCVGRKGRDLLRSDFGKKIEQKFIGVTGASKVEFADASKVTE
                     YVLERFDAGGFDVCHLVYNEFVSVLTQKPSVQQIIPFALPESEGVEKAPKKAGKTKEG
                     LSSPYDFEPEEEQILNALLPRNIGVQIFRSLLDSSAGEQAARMTAMDNATRNAGEMID
                     SLTLAYNRARQAYITKELIEIISGAEAV"
     gene            complement(4134..4403)
                     /locus_tag="PK513_08100"
     CDS             complement(4134..4403)
                     /locus_tag="PK513_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82448.1"
                     /translation="MENDVLGDLEKAFTAARRCGALCVDSHVFDDDRGGRMELLIAFS
                     SADHGEPRGIPLDDPKHAALVYAMGRALSGPLYAQRDGEVSLCQA"
     gene            complement(4407..5954)
                     /gene="atpA"
                     /locus_tag="PK513_08105"
     CDS             complement(4407..5954)
                     /gene="atpA"
                     /locus_tag="PK513_08105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOO82449.1"
                     /translation="MEIKAAEISEILKKQIADFGAQADVAEIGKVLSIGDGVARVYGL
                     DNVQAGEMVEFPGSIKGMALNLEADNVGVVVFGDDRNIKEGDIVRRTGKIVEVPVGKE
                     LLGRVVDGLGNPIDGKGPIKAKQTSRVEVKAPGIIPRQSVFEPMQSGLKAVDALVPVG
                     RGQRELIIGDRQTGKTAVAIDTIINQRTINKASDEKKHLYCIYIAIGQKRSTVAQLVK
                     ELEEQGAMEYSIVVAATASDPAPMQYLAAYTGAAMGEYFRDNGLHGLVVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYIHSRLLERAAKMSNDQGGGSLTALPIIETQAGDV
                     SAYIPTNVISITDGQIFLETGLFYKGIRPAINVGLSVSRVGSAAQIKAMKQVAGSIKL
                     ELAQYREMEAFAQFASDLDASTQKLLARGERLTVLLKQPQYSPLTVEEQVVVIFAGTK
                     GYLDGIAVSDVETYEARLLETLRSSGQGLLDRLAEEKKISDELEAKLREFIGDFTNGF
                     AGEKAAA"
     gene            complement(6010..6387)
                     /locus_tag="PK513_08110"
     CDS             complement(6010..6387)
                     /locus_tag="PK513_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOO82450.1"
                     /translation="MNEPLLLKDEVYQITGCAMSVLNELGRGFAEKVYENALAVEFGL
                     KDIPFSQQPGFEVHYKSQNVGLYVPDFIVFGKVIVELKTIDKIGSNEQGQVLNYLKAT
                     GLDVGLILNFKHSKLDWKRIVLS"
     gene            complement(6428..>6888)
                     /gene="atpH"
                     /locus_tag="PK513_08115"
     CDS             complement(6428..>6888)
                     /gene="atpH"
                     /locus_tag="PK513_08115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOO82451.1"
                     /translation="ASDDLRGAIRSPLYAEDSLLKAMGTLADHAKFQDITKNFLGLLV
                     KNGRLGGLPKILEAFAAALDKRRGAIAVNVDVAQDLSAKQQKELEAALSKAIGKEVAV
                     CANVKPDILGGMVVTVGSYMIDDSVRRKLERLKVSMGGGANENVSLKEVG"
BASE COUNT         1828 a         2062 c         1337 g         1661 t
ORIGIN      
        1 gggcaaagga agaaaatctt gttgattttc tccaagaaat gccttgtcat actcttcgct
       61 catgatccta tccaatgttt gaagataagc ctgcatttca ggcaattctt caatccctaa
      121 aatcgtagca agatctattg caaaatacac gggcacaagc tcgtcccgcc gcgcggcata
      181 cccttcaaga aactgcttat caaccgcatt ttctggacgc tcaacgccta aaaagtgcat
      241 aatactcatc ttaagataat catcggaata ataacgatca aaccattccg gctcttccgc
      301 tatcatttgc ccgtgcaact caaaaacatc tcttaaaaaa atccctgtct tataaatact
      361 ttcataaatt ttccgagaaa cattttgccg gtctacagtg ccacaaaaca atgtttcata
      421 tttttccgcc agcgccgtgc gcaaagcacc ataacgatcc gccagatttt taatttcaag
      481 accgttaaaa ccaagcgtcc gcttcgatag agaatacaac gccggatcct ccgcagtaat
      541 ctgattgtaa tcattcatcg catattcatg cgccatcttt ccagagcaat caaaattcgt
      601 tgttggctct gcatcaggag taagcgacaa aatatcatta ttttgcgccc cctcagccgc
      661 ctgcgcaaac gcagcgctgg cactaacact caaaacaaca gcaaccaaag cagcaggaac
      721 ttttttcatg aaaattgaat ccttttttca aattaaaaaa cggaccataa agaaaataat
      781 agcgttatgt caataaaata aagacttaag ccgccagttt gcccgtcacg gcggaaagct
      841 tggccttaag cagcaccaga cgcttttcga tgcgcgcctt gtcggcggct tcctcggcga
      901 tacccagatc ttcattgaga tcggcaattt tctgctcaat ctttgtttga tcgaggtcgc
      961 tcacaagaac ggcttcttca gccagcaccg tgcattgctc cgcgcttacg tccgcaaacc
     1021 cgcccgcaat aaaaatcttg cgcgcctcgc caccggcctg ctcataaagc tcaacaacac
     1081 cgggacgcaa agacgctacc aacgaagcat gcccggccag cacgccaaac tccccttcat
     1141 caccgggaat aacagccata tgcaccggct gggaaaccag cttttcctca ggagacacca
     1201 gttcaaattg aatattattt tccataatac tgaccctttt tggtttgcgt tgtcgttaat
     1261 ttacttaagc ttacttagct ttctgaatat tttctttgct caataagttt ttgcatgtac
     1321 cgcacctcaa actgtaaaac gggatgcaga taatccaaaa accacaagtc aacaaaatca
     1381 ttaacaatgt acccaacccc atgggccgca caggaagaac ttctttttcg caattgttac
     1441 aatacacttt gttcataact aaaatctcac ccttactgtc atccccgcac aaggcgggga
     1501 tccggatgcc cgcataaaag cgggcatgac agaatgaaag ttatgccgcc tctgccgcca
     1561 ttttcttggc tttttcaacc gcctgatcga tcgtgccaat catatagaac gcgctttccg
     1621 gcaggtggtc gtattgcccg tcaacaatcg ctttaaagcc tttgatcgtg tcttcaagct
     1681 gacagaacac acccggcgta ccggtaaaga cctcggccac atggaacggc tgtgacaaga
     1741 aacgctggat tttccgcgcg cgcgccacaa ccagcttgtc ttcttcagaa agctcatcca
     1801 tcccgagaat agcgatgatg tcctgcaggt ttttatattg ctgcaaagtc tgctgaacgc
     1861 ggttggccac gtcataatgc tcctgtccga tgatacgtgg gtcaagaata cggctggtcg
     1921 aatccagcgg atccacagcc ggataaatac ccatttccga aattgcacga gacagcaccg
     1981 tcgtagcgtc caggtgcgca aatgttgtgg ccggagccgg atcggtcaag tcatccgcag
     2041 gcacgtaaac cgcctgaatg gaggtgatcg agcctttatt cgtggacgta atgcgctctt
     2101 gcatattccc catctcggtg gacagtgttg gctgataacc cacagcggac ggaatgcgcc
     2161 ccagcaacgc agacacttcg gaacccgctt gtgtaaagcg gaagatattg tcgaggaaga
     2221 acaaaacgtc ctgaccttct tcgtcgcgga aatattccgc catggtcagc gcagacagcg
     2281 caacgcgcgc acgcgcgccc ggcggctcgt tcatttgacc gaacacaagc gccactttgg
     2341 agtttttgta atcatgctct ttaataacgc ctgcatccat catttcgtgg taaaggtcgt
     2401 tcccttcgcg ggttcgctca ccgacgccgc caaacacaga cacgccgccg tggcctttcg
     2461 cgatattatt aatcaattcc tgaatggtca cagtcttacc cacgcccgcg ccgccaaaca
     2521 gaccgatctt accgcctttg gcataaggac acagcaggtc aacgacctta atccccgtca
     2581 caagctgctc aacttcggtc gcctgatctt caaacgccgg cgctgcgcgg tgaatttccc
     2641 agcgcgcttt tgttttcagc gcaggccctt catcaatcac ctcacccgtc acattcataa
     2701 tgcgtgaaag cacttccggg ccaaccggca cagagatccc cgcgcccgtg tcaaccactt
     2761 ccgcgccgcg caccagaccg tctgtcatat ccatcgcaat cgcgcgcaca gtgttctcac
     2821 caaggtgctg tgccacttcc agaaccaggc ggttgccctg attgtccgtc tccagcgcgt
     2881 tcaaaatcgc cggcacttcg ccttcgttga actgcacatc cacaaccgcc cccaaaacct
     2941 ggagaatagt gccaaccgca cctgttgctt tgctttttgc tttcgctttc gccatttttt
     3001 tgtcctctta tcgtttaact cttactcacc cggggcacgg gtgaaattca tactgtctgt
     3061 cccgctttca cgactaggaa acacaggctt tccgctcgct gtgcgacgag atttaaccca
     3121 ccatccatct ctacgtcttt cagcagtttc ctgcacttct tgccaatccc tattaacagc
     3181 aactttaact ttaacttcat cagtcatatc aaatactcct tctactcaat cttacaccgc
     3241 ctccgcgccg gagatgatct caatcaattc cttggtaatg taagcctgac gcgcacggtt
     3301 ataggccagc gtaagactat caatcatttc cccggcattg cgcgtcgcat tatccatcgc
     3361 cgtcatccgc gccgcctgct caccggcaga gctatccagc aagctgcgga aaatctgcac
     3421 accaatattc cgcggcaaca aggcattcaa aatctgctct tcctctggct caaaatcata
     3481 cggagagctt aaaccctctt tggtctttcc cgcctttttc ggagcttttt ccaccccttc
     3541 gctctccggc aaagcaaaag gaataatctg ctgcacagaa ggcttttgcg tcaacacgga
     3601 aacaaattcg ttataaacca gatgacacac gtcaaatccg ccagcatcaa accgctccaa
     3661 cacatattcg gtaaccttgc tggcatcggc aaactcgacc tttgacgctc ccgtgacccc
     3721 gataaacttt tgctcaattt tctttccaaa atcactacgc agcaaatccc gtcccttacg
     3781 cccgacacag acaattgaaa catccttgcc catgtcttga agttctttaa tcttcgcccg
     3841 cgctttctta acaaggtttc cattaaaccc gccgcaaagc ccacggtcag ccgtcacaat
     3901 caccagcaaa tgcttctgat cactgcccgt tccagccagt aatggagaac tcgcatcgct
     3961 caccacaacg ccgccagcaa cgcgcgccat catatcggcc atgcactgcg cataaggctg
     4021 agacgcttcg gcttgttcct gcgccttgcg caacttggat gccgcaacca tcttcatcgc
     4081 agaggtgatc ttgcgggtcg atttaacgga cgctatccgg tctctataat ctcttaagct
     4141 tggcataacg acacctcacc atcacgctgg gcgtataatg gaccagacag cgctcttccc
     4201 atagcataaa ctaaagcagc atgtttaggg tcatcaaggg gaataccgcg cggttcacca
     4261 tgatcagcag aagaaaaagc tatgagcaac tccattctcc caccccggtc atcgtcaaac
     4321 acatgagaat ccacgcacaa cgcaccacat ctacgagccg cagtaaaagc tttttccaaa
     4381 tctcccaaaa catcattttc catagcttaa gctgccgctt tctcaccagc aaaaccattg
     4441 gtaaaatcac caataaattc acgcagcttg gcttccagct catcagaaat tttcttttct
     4501 tccgcaagcc tgtcaagaag accctgcccc gatgagcgca atgtctccag caagcgcgcc
     4561 tcataagtct ctacatcgga aacggcaatc ccgtcgagat aacctttagt tccggcaaaa
     4621 atcactacaa cctgttcttc aacggtcagc ggagaatatt gcggctgctt caaaagcacg
     4681 gtcaaacgct caccacgcgc cagcaatttc tgcgtcgagg cgtccagatc agatgcaaac
     4741 tgcgcaaacg cctccatttc gcgatattgc gcaagttcaa gtttaatcga acctgcaacc
     4801 tgtttcatcg ccttgatctg cgccgcagag ccaacgcgtg acacagacaa accaacgtta
     4861 atcgccggac gaataccttt ataaaacagc cccgtctcca ggaagatctg accatcggtg
     4921 atcgaaatca cgttggttgg aatataggcc gacacatcac cggcctgtgt ttcaatgatt
     4981 ggcaaagcgg tcaaagaccc gccaccttga tcatttgaca ttttcgccgc acgctccagc
     5041 aagcgagagt gaatatagaa cacatcgccc ggatatgctt cacggccagg aggacgacga
     5101 agcagcaagg acatttggcg atacgctacg gcctgtttcg aaagatcgtc ataaacaact
     5161 agaccatgca gaccgttatc acggaaatac tcacccatgg ccgcgccggt ataggccgcc
     5221 agatattgca tcggcgctgg atcagaagcc gtcgcagcga ccacgataga atattccatc
     5281 gcaccctgct cttcgagttc cttcacaagt tgcgccacag tcgaacgctt ctgaccgatg
     5341 gcaatgtaaa tacaatacaa atgcttcttc tcatcagacg ctttgttgat cgtgcgctga
     5401 ttaatgatcg tatcaatcgc aacagccgtt ttcccggtct gacggtcgcc aataatcaat
     5461 tcgcgttggc cccggccaac aggaacaagc gcatcgacag ccttaagacc tgattgcatc
     5521 ggctcaaaca cagattgacg aggaataatc cccggcgctt tcacttcaac gcggctggtt
     5581 tgctttgcct tgatcggacc tttaccgtca atcggattcc ccagaccgtc cacaacgcgg
     5641 cccaaaagct ctttcccaac aggaacctca acgatcttcc cggtccggcg cacgatgtcg
     5701 ccttctttaa tgttacggtc atcgccaaac accacaaccc cgacattgtc ggcctcaagg
     5761 ttcagcgcca tacctttaat actgccagga aattcaacca tctccccggc ctgaacatta
     5821 tccagcccgt aaacgcgcgc cacaccgtca ccgatggaca aaaccttccc aatttcggcc
     5881 acatccgcct gcgcaccaaa atcggcgatc tgttttttga gaatctcgga gatttctgct
     5941 gctttgattt ccattatttc aaatcctctt tcttataaac cgcgaataca cacgaatgaa
     6001 cgcgaatatt caagataaaa caatacgttt ccagtctagt tttgaatgct taaaatttag
     6061 gattaaacca acatccaaac ctgtcgcctt caaataattt aaaacctgtc cttgctcgtt
     6121 cgaaccgatt ttatcaattg tttttaactc aacaataacc ttaccaaaca ctataaaatc
     6181 aggaacataa agcccaacat tctggctttt atagtgaact tcaaaaccgg gctgctgaga
     6241 aaacggaata tcttttaaac caaactcaac agccaacgcg ttttcataga ctttttccgc
     6301 aaaaccacgg ccaagctcat taagaacaga catagcacac cccgttatct gataaacttc
     6361 atcctttaac aataacggtt cattcatagt cataaattcg tgtctattcg tgttcattcg
     6421 cggtttttta cccaacctct ttcaatgaga cattctcgtt cgcaccgccg cccatggaga
     6481 ctttcaaacg ctccagcttc cggcgcacgg aatcatcaat catataagac ccgactgtca
     6541 ccaccatacc gcccagaata tctggcttca cattcgcgca cacagcaacc tctttaccga
     6601 tcgccttgga caatgccgcc tccagctctt tctgctgctt cgcagacaaa tcttgcgcca
     6661 catcgacatt cacggcaatc gccccgcgtc gcttatccag agccgccgca aaggcttcaa
     6721 gtattttggg caaaccgccc aaccggccat tcttaaccaa cagccctaaa aaatttttgg
     6781 taatatcctg gaatttagca tgatcggcca aagtccccat agctttcaac aaagaatcct
     6841 cagcataaag cgggctacga atcgccccgc gcaaatcatc ggaagcct
//
LOCUS       DAOSWR010000109         6844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7215_length_6844_cov_4.738548, whole genome shotgun sequence.
ACCESSION   DAOSWR010000109 DAOSWR010000000
VERSION     DAOSWR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7215_length_6844_cov_4.738548"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(409..1053)
                     /locus_tag="PK513_08120"
     CDS             complement(409..1053)
                     /locus_tag="PK513_08120"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOO82452.1"
                     /translation="MSVFSKPHLLNSPRWKNMRVGLLGGSFNPPHAGHVHISLAALKG
                     LQLDAVWWLVSPQNPIKDVKALPLAKRIALCQKLVDHPKILVSDIEKDLGTSITYHSI
                     RKLKRRYPCAQFVWISGMDNALSLHKWHYWRELLDEIPMLHLTRSPARSLVSGCPYRM
                     YGHQKHMVIDHGSAYPLDSGISYWMMQKKMVNISSTEIRHNALKNNNKATKTAD"
     gene            complement(1072..2349)
                     /locus_tag="PK513_08125"
     CDS             complement(1072..2349)
                     /locus_tag="PK513_08125"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOO82453.1"
                     /translation="MSEALFQQSPQETVHTLGMQAKEASRILVQTSESNINAILNALA
                     VKLRDNVPILLEGNAKDLAAGEQKGLSPALLDRLALNEERIEGMAAGVETVANLPDPV
                     NRVLWETERPNGLKIQRVVVPIGVLGMIYESRPNVAIDAAALCLKSRNAVILRGGSES
                     LHSSLALHRLIQETLKEHRLPEGAVGMIPVPDRAAVDAMLAADQYIDVMIPRGGKGLI
                     ERVQNHARMPVFSHLDGICHVYIHKDADPQIAVEVTRNSKLRRTGLCNAAETLLLDQK
                     LDPEIAKAVLSKLLDEGCEIVGDKMAQALDSRIGVASAHDWGMEYLDKKISCAVVGGI
                     EEAVNHINTYGSHHTDSILTQDEETAAYFFKNVDSGSVMHNTSTQFADGGEYGFGAEI
                     GIATGKMHARGPVGLEQLCTYKYLVHGTGQTRP"
     gene            complement(2366..2854)
                     /locus_tag="PK513_08130"
     CDS             complement(2366..2854)
                     /locus_tag="PK513_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82454.1"
                     /translation="MTFLEDPSIWLLCSFIIFAGLIFRFGKNALLSMLDQRIASIREE
                     IKTAENLRVEAQEMLAQYQRKHKDAVKDAENIIANAQKQAKQIQKKAEDDLDEVIARR
                     EKQLQERLERMKQNAQAEILEYASSLAIAATREIISDKLDKKANEKLVAQAIKNVGKN
                     LH"
     gene            complement(2867..3589)
                     /locus_tag="PK513_08135"
     CDS             complement(2867..3589)
                     /locus_tag="PK513_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82455.1"
                     /translation="MNTTFLKFGAQASMALLWASEAFAADSEEFIGKDSSAPDYIVED
                     GAVHAVDKRGEAAIHVGEHAGEHVDVAHHASSGLPQMDPTWFPSQIFWLAVTFLCLYV
                     ILSRKILPEISGTLEARRMQIQEDLDSAQSLKEEAEDVHQAYDEILDGARKKASELFE
                     RAEEDIKTTTNKKLDELRERANKENTETEKQIEKAKTDAMADMYAVAAEIASLAAEKI
                     VGISTDIDQAKSLVKNIDKKAA"
     gene            complement(3602..3826)
                     /locus_tag="PK513_08140"
     CDS             complement(3602..3826)
                     /locus_tag="PK513_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOO82456.1"
                     /translation="MEVEAAKLIGAAIALIPLLGVGIGLGLIFASYNDAIARNPKSDE
                     ILGGKYLLFFALTEALAIFALGVSLLILFK"
     gene            complement(3923..4669)
                     /locus_tag="PK513_08145"
     CDS             complement(3923..4669)
                     /locus_tag="PK513_08145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOO82457.1"
                     /translation="MADPIHQFVITPIIPFEVSGFDLSFTNSALWMAIGAFVSSSFLM
                     LAMRKQAIVPSRMQAFSEMLYEFVAGMVRDNVGSHGRQYFPLIFTVFIIVLMGNVLGL
                     LPYSFTYTSHIIVTGVLALMIFLIVTAFGFFNHGLKFLTLFTPPGVPFVMQLLIVPIE
                     VLSFLIRPATLSLRLFANMMAGHLMLKVFAGFSTMMLGLGGIGLIAGLIPMFFNVALY
                     SLELLVAVLQAYIFAILSCIYLKDTVDLHH"
     gene            complement(4675..5043)
                     /locus_tag="PK513_08150"
     CDS             complement(4675..5043)
                     /locus_tag="PK513_08150"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOO82458.1"
                     /translation="MNSDLKDLEKKIREAKHGKDPDAEELSRIRKAQNSKMAMQAGYE
                     FVASVMLSAILGYFIDQWLGTAPLFLISLFLLGTCAGFLSIFRVSKNLGMGVGYSQLH
                     RDEKDDNKAPNANSNERTEE"
     gene            complement(5054..>6844)
                     /locus_tag="PK513_08155"
     CDS             complement(5054..>6844)
                     /locus_tag="PK513_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOO82459.1"
                     /translation="KGADELRPHVKAPKVLNIALSQIGVVETVKDGDALWESLKPGQA
                     LVSKDGTYWRWDGYCVKSSAPDRHAQQLEQQNKLESLIKKRPQIEKDVAVAEKALESA
                     QGQRVSLREKLQKTQSAMRACEAGLNEVRNEWSSLREEHARMQSEILRLEDVIAVTAQ
                     EIDELESALESDAKELTAYESADEENKEQKLEALRETLQEARQAHGEAVRAYDIYVQE
                     RSTRNARMQAIGDERLHLQNRAIRARERIKELNERTQALSGKLESLDSRPDDFEEDRA
                     KLLNMISELEDKRGDAADKLARVENEVAETAKALKEAEGVLGEAREARAHAQALMSSM
                     QEQRGAMSLSIEEKFDMKPEELPEHSAIDMDKNLGDLDSLKGKKENLIRERDNIGAVN
                     LRAEEEAAELEKEAGGLIHERNDLVQAIEELRSGIQKINVEARERLLAAFEHVNAHFQ
                     GLFVRLFGGGQAHLALVESDDPLNSGLEIFAQPPGKALQSLSLLSGGEQTMASIALIF
                     AMFLTNPSPICVLDEIDAPLDDANVDRVCDLLEEIAERGETRFLVITHHRLTMARMHR
                     LYGVTMAERGVSQLVSVDLQQSFDFLEEAA"
BASE COUNT         1731 a         1860 c         1475 g         1778 t
ORIGIN      
        1 catataatca gccaatccgg tttgctcatc cagtttaatg ctgacaatat cttcggcctt
       61 atctgcatct aacgactgct cgataaggct tttcagttca ctaggggtta agcgtccttt
      121 ggaaccgtga tgcggatcat gggttaaaaa ggccgaggcc tcctttcttg ttttatatta
      181 gtttctacga acataaaaaa agctgaacgc aaaggcccag cgggtgctgt gcacatacag
      241 gcacaagatc tgtggtccac aacgtactca caacgccgcc caatgagatt atgctgcatt
      301 gaacccccat cggtttgatc tgctactgcc gtcacattta gaccacatac tatcattata
      361 ggccgtttag ccctgcgcgg caacaatcat ccttgtcgca gtgcacgttt aatcggccgt
      421 ttttgttgct ttattattat ttttcaaagc attatgacga atttcagtcg aagatatatt
      481 gaccattttt ttctgcatca tccagtagga tatgcccgaa tcgagcggat aagcactgcc
      541 atgatcaatg accatgtgtt tttgatgtcc atacattcgg tacggacacc cggaaaccaa
      601 ggaacgcgcc gggctgcgcg tcagatgtag catcggaatt tcatcgagca attcacgcca
      661 gtaatgccat ttatgtaagg agagcgcatt atccattccg ctgatccaga caaactgtgc
      721 acacggataa cggcgtttaa gcttgcgaat ggagtgatag gtaatcgacg tacccagatc
      781 cttttcaatg tcggaaacaa gaattttcgg atgatcaaca agcttctgac ataaagcaat
      841 acgcttagcc agaggaagag ctttaacgtc cttaatcggg ttttgcggac tgaccagcca
      901 ccacaccgca tcaagctgca accccttaag cgccgccagg gaaatatgca catggcctgc
      961 atgtggcgga ttaaacgacc cgcccagcaa acccacgcgc atattcttcc agcgcggtga
     1021 attcagtaaa tgcggctttg aaaagacaga cataatggcc agtgtggctc gtcagggccg
     1081 ggtttgtcca gtgccgtgta ccagatattt gtaagtgcat agctgctcaa gccctacagg
     1141 cccgcgcgca tgcattttac ctgtcgcaat gccgatttcc gcgccaaaac cgtattcgcc
     1201 gccatcagcg aactgtgttg aggtattatg catgacgctg ccgctatcga cattcttaaa
     1261 gaaatatgcc gctgtctctt catcctgtgt caaaatactg tccgtatgat gcgatccgta
     1321 agtattgata tggtttacag cttcttcgat tccgcccaca acggcgcaac tgattttctt
     1381 atccagatat tccattcccc aatcatgcgc tgaagctacg ccaattcgac tatccaaggc
     1441 ctgagccatc ttgtcgccaa caatttcgca gccttcatcc agaagttttg aaagcacggc
     1501 ttttgcaatt tccggatcaa gtttctggtc aagcagcaac gtttccgcgg cattgcacag
     1561 gcccgtacgg cgcagcttcg aattacgcgt gacttcaaca gcaatttgcg gatcggcgtc
     1621 tttgtgaata tagacatggc aaataccgtc cagatgactg aataccggca tgcgggcatg
     1681 gttttgaacg cgctcaataa gccctttgcc tccacgcggt atcatgacgt cgatatattg
     1741 atcagccgcc agcatggcat caaccgccgc gcgatcagga accggaatca tgcccactgc
     1801 gccttccggc aatctatgtt cttttagtgt ttcctgaatg aggcggtgca acgccagcga
     1861 gctatgcaaa ctttccgaac caccacgcaa gatgaccgca ttacgagatt ttaaacacag
     1921 cgccgccgca tcaatggcta cattcggcct ggattcataa atcatcccca atacaccaat
     1981 cggcactaca acgcgctgaa ttttaagacc gtttgggcgc tcggtctccc acaaaacacg
     2041 gtttacaggg tccggcaaat tcgcaaccgt ctcaacacct gcggccatgc cttcaatccg
     2101 ctcctcattc aaagcaagcc gatcaagcag ggcaggggaa agtccttttt gttctccagc
     2161 agccaaatcc ttggcgttac cttccaaaag aatcggaaca ttgtcccgca acttcacagc
     2221 taaagcgttc aaaattgcgt tgatgttgga ttcgctcgtt tgaacaagaa tgcggctggc
     2281 ctctttggcc tgcatcccca atgtgtgcac agtttcctgt ggggattgct ggaaaagggc
     2341 ttcggacata ggcaggcctc aagaattaat ggagattttt accaacattt ttgatcgctt
     2401 gcgccaccag cttttcgttg gcttttttgt caagcttatc ggagataatc tccctggtcg
     2461 ctgcaattgc cagagaagag gcatactcaa gaatctcagc ctgcgcattt tgcttcatac
     2521 gctcaagacg ttcctgtaac tgtttttcac gccgtgcaat cacctcatcc agatcgtctt
     2581 ccgctttttt ctgaatttgc ttggcttgct tttgtgcgtt ggcgataata ttttcagcat
     2641 ctttgaccgc atctttatgc ttgcgctgat actgcgccaa catctcctga gcctcaacgc
     2701 gcaaattctc cgctgttttg atttcctcac ggatactcgc aatacgctga tccagcatgg
     2761 aaagcagggc atttttaccg aatctaaaaa tcagccctgc aaaaataatg aaagaacata
     2821 gaagccaaat actaggatct tcgagaaatg tcattacctt tgtcctttac gctgcttttt
     2881 tgtcaatgtt cttaaccaga gactttgcct gatcgatgtc cgtagatatg ccgacgattt
     2941 tctctgctgc cagactggca atttctgctg caactgcgta catgtcggcc atcgcatcgg
     3001 ttttggcttt ttcgatttgt ttttcagtct ctgtgttttc tttgtttgcg cgctctctaa
     3061 gctcatcgag ctttttattt gttgtggtct ttatatcttc ctcagctctt tcaaacagtt
     3121 cggaggcttt cttgcgcgca ccatcgagaa tttcatcata ggcctgatga acgtcttccg
     3181 cttcttcttt taagctttgc gcactatcaa gatcttcttg aatttgcata cgacgagctt
     3241 ccaaagtgcc agagatttcc ggcaaaatct tccgtgataa aatcacatac agacatagga
     3301 aagttactgc gagccaaaaa atctggctgg gaaaccacgt agggtccatt tgcggcaaac
     3361 ctgatgaagc atgatgtgcc acatccacat gctcaccagc atgttcgcct acatgtatag
     3421 ccgcttcacc acgcttatcg acagcatgca cagccccatc ttccacgata taatcaggcg
     3481 cagaagaatc cttgccaata aattcttcac tatcggcagc aaaagcctca ctggcccata
     3541 agagggccat agaggcttgt gcaccaaatt ttaaaaaagt ggtattcata ccatctccgt
     3601 cttacttgaa gaggatcagc aaagacacac caagagcgaa aatagccagc gcttctgtca
     3661 aagcgaagaa cagcagatat ttaccgccaa ggatttcatc agacttaggg ttgcgggcga
     3721 tcgcatcatt gtaagaggcg aaaatcaggc ccagaccaat accaacaccc agcaaaggga
     3781 taagggcaat cgcagcacca atcagttttg cagcttctac ttccattgtt aataaccttc
     3841 ctttttagtt taaatggttg ttaaagttga attcgtaaat tctttcccgt tatttgcagc
     3901 atttgcagcg catcctacag ttttagtgat gaagatcaac cgtatctttt aaataaatac
     3961 agctcaaaat cgcaaaaatg taggcttgca atacagccac gagaagctca agagaatata
     4021 gcgcgacgtt aaagaacatc ggaattaaac cggcaatcag cccaatgccg ccaagtccaa
     4081 gcatcatcgt gctgaaaccg gcaaatactt tcagcatcag gtgtccggcc atcatgttgg
     4141 caaacaaacg taaggacaag gttgcaggac ggattaagaa agacagcact tcaatcggca
     4201 cgatgagaag ctgcatgaca aacggtacgc ctggcggggt aaacaaagtc aaaaatttca
     4261 ggccatgatt gaaaaatcca aaggccgtaa cgatcaaaaa gatcatcagc gccaaaacgc
     4321 ccgtcacaat aatatggctg gtgtatgtaa aagagtaggg aagaagcccc aaaacattgc
     4381 ccatcagcac gataataaag acggtgaaaa tcagcgggaa atattgccgt ccgtgtgagc
     4441 cgacattatc gcgcaccatg ccggcgacaa attcatacag catttctgaa aaagcctgca
     4501 tacggcttgg aacgatcgcc tgcttgcgca tcgccaacat caaaaacgaa gacgatacaa
     4561 acgcgccaat ggccatccag agggctgaat ttgtaaaaga caaatcaaaa cctgaaacct
     4621 caaaagggat aatgggggta atcacaaact gatgaatagg atcggccacg gcgcttactc
     4681 ttctgttctc tcgttgctat tggcgtttgg cgctttatta tcgtcttttt catcgcgatg
     4741 caactgcgaa tatcccacgc ccatacctaa atttttggat acacggaaaa tggacagaaa
     4801 acccgcgcac gtgcccagta aaaacaagga gataaggaaa agcggcgccg tccctagcca
     4861 ctggtcgatg aaatagccca aaatggcgga taacataacg gaagctacaa attcatagcc
     4921 cgcctgcatg gccattttgc tgttttgcgc ttttctgatt cgcgaaagct cttctgcgtc
     4981 cgggtcttta ccgtgtttag cctcacgaat tttcttttct aaatctttca ggtcagaatt
     5041 catgctcgcg gttttaagct gcctcctcaa ggaagtcaaa actttgctgc aaatccacgg
     5101 agacaagctg cgagacgccg cgctcagcca ttgtcacgcc gtacagccgg tgcattcgcg
     5161 ccatcgtcag acggtggtgg gtaatcacca gaaaacgcgt ttcgccgcgc tcggcgatct
     5221 cttcaagcaa atcacacacg cgatcaacat tggcatcatc cagcggcgca tcaatttcgt
     5281 ccaaaacgca aatcggcgag gggttggtca aaaacatcgc aaagatcagc gcaatcgagg
     5341 ccattgtttg ttcgccacct gatagtagag acagggattg cagtgcttta cccggcggct
     5401 gggcaaaaat ctcaagaccc gaattgagag gatcatccga ttcaaccagc gccagatgtg
     5461 cctgaccacc accaaacagc cttacaaaca acccctggaa atgcgcgttt acatgctcga
     5521 aagctgccaa taaacgctct cgcgcctcga cgttgatttt ctgaatgccg ctgcgcagct
     5581 cttcaattgc ctgcaccaga tcattacgct catggattag cccaccggcc tctttctcga
     5641 gttccgccgc ttcttcctcg gcccgcaaat tcactgcgcc gatattatcc cgctcgcgga
     5701 tcaggttttc tttcttgcct ttcaacgaat ccagatctcc cagattctta tccatatcaa
     5761 tcgccgaatg ctctggcagt tcttcgggtt tcatatcgaa tttttcctca atgctcaggc
     5821 tcatcgcgcc gcgctgttcc tgcatcgaag acatcagcgc ctgggcatgt gcccgtgctt
     5881 cccgtgcttc acccaaaacg ccttccgctt ctttcaatgc cttggccgtt tcggcaactt
     5941 cattctctac gcgtgccagt ttgtctgcgg catcacctcg cttatcctcc aactctgaaa
     6001 tcatattcag caatttggct ctatcctctt cgaagtcatc tggacgagaa tcaaggcttt
     6061 cgagcttgcc agacagagcc tgggtccgtt cattcagctc tttaattcgc tcccgcgcac
     6121 gaatagcgcg gttttgtaga tgcagccgct cgtcaccaat tgcctgcatc cgggcgttgc
     6181 gtgtacttcg ctcttgcaca taaatgtcgt aagcgcgcac cgcttcgcca tgagcttgcc
     6241 gggcctcttg cagtgtctcg cgtaaggctt caagcttctg ttctttattc tcttcatcgg
     6301 cactttcata cgccgtcagt tccttggcat cactctccag cgcgctttcc aactcgtcga
     6361 tctcctgcgc tgtcaccgca atcacatcct ccagccgcag gatttcactc tgcatgcggg
     6421 catgctcttc acgcaaagat gaccactcat tacgaacttc attcaggccg gcctcacaag
     6481 cacgcatcgc gctttgcgtt ttttgcagct tttcacgcaa gctcacgcgc tggccttgtg
     6541 ccgattccag ggctttctcc gccactgcca catctttttc aatttgtggt cgcttcttaa
     6601 tcaggctctc aagcttgttt tgctgctcaa gctgttgtgc atgacgatca ggcgcagatg
     6661 attttacgca atatccatcc cagcgccaat aagtcccgtc cttggagacc agcgcctgac
     6721 cgggcttgag cgattcccac aaagcatcgc catccttcac cgtttccacc acgccaatct
     6781 gcgacagcgc gatattcaaa actttcggcg cctttacatg cgggcgcaat tcatccgcac
     6841 cttt
//
LOCUS       DAOSWR010000110         6725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7409_length_6725_cov_4.461619, whole genome shotgun sequence.
ACCESSION   DAOSWR010000110 DAOSWR010000000
VERSION     DAOSWR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7409_length_6725_cov_4.461619"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK513_08160"
     CDS             complement(<1..384)
                     /locus_tag="PK513_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82460.1"
                     /translation="MTKINIDKIASVTTNLALKHEEKLTDKLSCEMGTVLAVQVLEDK
                     SIYNELELPTGRMSKLKKGDILAVALGNRMALKGFVGRLPKKLKVDDIIHLLNFGGVA
                     GVCTSANVHEVGEPLRIRVLGGIERK"
     gene            complement(604..1803)
                     /locus_tag="PK513_08165"
     CDS             complement(604..1803)
                     /locus_tag="PK513_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOO82461.1"
                     /translation="MSASIRNSIQTLKDEIAEYRQALHENPQTAYEETFASGLVTEKL
                     TEWGIPFERGIAVTGIVATIEGERNTSGKAIGLRADMDALDIEEADNKPHRSKNPGKM
                     HACGHDGHTACLLGAAKYLSENRNFDGRVHLIFQPAEEGGRGAHRMIEEGLFKRFPCD
                     SIYGLHNWPQLPKGTVATRPGPVMASVDILHIVVKGKGGHAAMPHKVVDPIVTGSHII
                     TTLQTLVSRYRPPTEPVVLSITYFNAGTGAHNIIPNEARLTGTLRTYDESLRQALKIK
                     ICETARGIAKAMGGDIEYEFLEVLDPTINTPQETEFCANIAREIFGEHNVDADVTPSL
                     GGEDFGRMLFDIPGCYIWMGQGEEDPESNHSQGLHTPCYDFNDEIIPLAIEYWARIVE
                     KTLPLEK"
     gene            complement(1867..2037)
                     /locus_tag="PK513_08170"
     CDS             complement(1867..2037)
                     /locus_tag="PK513_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82462.1"
                     /translation="MAEYFKKQQILAILEKNQRLSVAPSPSETATAPSDAEESSEASL
                     NMAENAASPILT"
     gene            complement(2146..3171)
                     /locus_tag="PK513_08175"
     CDS             complement(2146..3171)
                     /locus_tag="PK513_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO82463.1"
                     /translation="MTFSPITQRRLSQFKANKRGFWSFWIFLSLLVIGLGAEFIANDK
                     PILIRYDGGYYFPTFQHYPETTFGGDFETETEYRDPFVMELIEAKGWMVWPPIRYSYD
                     TINYNLPQPAPSPPTGENIFGTDDQGRDVAARVLYGFRNSSTFGLILTLISSIIGIAV
                     GAIQGYYGGKIDLIMQRVIEVWSSMPQLYILIIFSSIFVPGFWVLLWILLLFSWISLV
                     DLVRAEFLRARNFDYVRAARALGVSNPVIMWRHVLPNAMVSTITFMPFILTGAITALT
                     ALDFLGLGMPPGSPSLGEVLRQGKTNLQAPWLGITAFVSLSLMLTLLTFIGEAVRDAF
                     DPRKTTL"
     gene            complement(3168..3299)
                     /locus_tag="PK513_08180"
     CDS             complement(3168..3299)
                     /locus_tag="PK513_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82464.1"
                     /translation="MNLNHRDTEAQSFYIVIASAAKQSSLDCRVGLAGLLAMTGEKE"
     gene            complement(3299..4405)
                     /locus_tag="PK513_08185"
     CDS             complement(3299..4405)
                     /locus_tag="PK513_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HOO82465.1"
                     /translation="MATYIFKRLLLMLPTLFGILLISFVIIQFVPGGPVEKMIAQLQG
                     HDMEATARFGGSSGGGDAVGAMSQTATGQGSKYRGAQGLDPEFIAELEALYGFDKPAY
                     ERFWIMLKNYARFDLGTSYYRDIGVLDLVLEKMPVSISLGLWSTLIIYLISIPLGIKK
                     AVRDGTPFDIWTSAAVFIGYAIPSFMFAILLIVVFAGGRYFDLFPLRGLVSDGWEEMS
                     FIELALDYMWHMVLPIAAMVISGFASLTLLTKNSFLDEINKQYVLTARAKGLSEKQVL
                     YGHVFRNAMIIVIAGFPAAFVSIFFTGALLIEVIFSLDGLGLLGYESVINRDYPVVLG
                     TLYIFALISLVMHLIRDIVYVMVDPRIDFEAKAA"
     gene            complement(4408..5025)
                     /locus_tag="PK513_08190"
     CDS             complement(4408..5025)
                     /locus_tag="PK513_08190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO82466.1"
                     /translation="MTTLIIARHGNTFGPRDTPTRVGRHTDLPLVEKGRAQAKAIGHY
                     LKENKMIPDVVYASHLKRTIETAEISIQESGVSNPVYQLGIFDEIDYGPDENKVEADV
                     IARIGKQALSDWDEHGLVPDGWKVDPDAIIQNWHNFADQIAAHDDDETVLVVTSNGIA
                     RFAPHITGDFEGFAAGHSIKLATGALGLLKHEDGLWRVLDWNVKP"
     gene            complement(5022..5792)
                     /locus_tag="PK513_08195"
     CDS             complement(5022..5792)
                     /locus_tag="PK513_08195"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manno-octulosonate cytidylyltransferase"
                     /protein_id="HOO82467.1"
                     /translation="MKTAIVIPARYGSTRFPGKPLAQIGGQSMLSRVVDLARSIENAD
                     VFVTTEDRRIADHATALGVECVMTPDDCPTGSDRVLAALRQCDEMPDFVVNLQGDAPF
                     TPLSVLQALIKAFENNPEVVTPVHRLSWDDFDRLREAKKTTPFSGTTAIVKDGRALWF
                     SKNIIPAIRKEDRSALLSPVYQHMGLYGFRVDILERFCALPQGYYEQLEGLEQLRMLE
                     NEIEVTAVPVDIDQGQIQSGIDTPEDLARAEEMLKALS"
     gene            complement(5793..>6725)
                     /locus_tag="PK513_08200"
     CDS             complement(5793..>6725)
                     /locus_tag="PK513_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO82468.1"
                     /translation="AQPVKDGRIVKEEIDNARPAGMQGFIYNIRRDVFKDRAVREALS
                     YAFDFEWSNKQFAYGTYTRTDSYFENSELASSGLPEGKELELLERFRGKIPDEVFTKT
                     YAPPVSDGSGNARANLRKATGLLNEAGWVLETDGVLAKDGAKLEFEILDANPMFERWV
                     LPFIKNLERIGVKANFRVVDSAQYQNRMNAFDFDMTISGFGQSSSPGNEQRDFWGSEK
                     AAIEGSRNYIGIQDPVIDQLIEELIQTSSREDLVAHVGAMDRILLWNHYVIPMWHYPK
                     WRIARWTHIERPDPLSDISPLISDTWWAKRKGAQ"
BASE COUNT         1748 a         1882 c         1479 g         1616 t
ORIGIN      
        1 tttacgctca attccgccca atacacggat tcgcagcggt tcgccaacct cgtgcacatt
       61 ggcgctcgtg caaacgcctg ctacgccgcc aaaattcaac agatgaataa tatcgtcaac
      121 tttaagcttc ttcggcagcc gtccgacaaa gcctttcaac gccatcctgt tacccagcgc
      181 cacggcaagt atatcacctt tcttaagttt gctcatccgc ccggtaggca gctccagctc
      241 attataaatg ctcttgtctt ccagtacctg caccgccagt accgtcccca tctcgcatga
      301 gagtttatcg gtaagctttt cctcgtgctt aagcgctaga ttggttgtca cggacgcgat
      361 tttatcaata tttatttttg tcatgttttc aatttcttat aggttacgac acaatgcaac
      421 gctttagcct ctcattcaag agtccatacc gtttaagcgt tcatatttta ccgaaaaatg
      481 ttaaggcaac aaaaaacgcc cgcttaaatc gatatgatta gcgagggcgt cgtcgggtag
      541 ttgtatgtgc aacagcgttg ttcataaacg ctataaagcg taaaaggtct gtacttgtca
      601 attttatttt tccaacggca gtgttttttc aacgatccgc gcccagtatt caatggctaa
      661 tggtataatc tcgtcattga aatcatagca cggcgtatgc agcccctggc tatggtttga
      721 ttcagggtct tcttcgcctt gccccatcca gatatagcag cccggtatat caaacagcat
      781 ccgcccaaaa tcctctccgc ccagtgaggg cgttacatcc gcatccacat tatgctcgcc
      841 aaaaatctct ctcgctatat tcgcgcaaaa ctcagtttcc tgaggagtgt tgatggtcgg
      901 atcaagaact tccagaaatt catattcaat atctccgccc atggccttgg caattccgcg
      961 cgcggtctca cagattttaa tcttaagagc ctgacgtaaa gactcgtcat aggtgcgcaa
     1021 agttccggtc agcctggctt cattggggat gatgttatgc gcgccggttc cggcattaaa
     1081 atacgtgatt gacagcacga ccggctcggt cggaggtcta taacgactga ccagtgtttg
     1141 caacgtcgtg atgatgtgag atcctgttac aattggatca accaccttgt gcggcatagc
     1201 ggcatgcccg cctttaccct taaccacgat atgcagaata tccacactgg ccatgaccgg
     1261 gcctggccgc gtcgccaccg tacctttagg aagctgcggc cagttatgca gaccgtaaat
     1321 ggaatcgcaa ggaaagcgct taaacaaccc ttcttcaatc atacgatgtg ctccgcgtcc
     1381 accttcctcg gccggttgaa aaatcagatg taccctgccg tcaaaattcc ggttttcgga
     1441 cagatatttt gccgcaccta gcaaacaggc cgtatgcccg tcatgtccgc acgcatgcat
     1501 cttgcccgga ttttttgatc tgtgcggctt gttatcagcc tcttcaatat ccagtgcatc
     1561 catatcggcg cgcaggccaa tcgctttgcc ggaagtattc cgttcgccct caatcgttgc
     1621 aacaatccct gtcacagcaa ttccgcgttc aaaaggaatt ccccactctg tcagcttctc
     1681 agtcacgagg cctgaagcaa aggtttcctc atacgccgtt tgcggatttt cgtgcagtgc
     1741 ctgccgatac tctgcgatct cgtcttttaa agtctgaata gaattacgaa tagaagcgct
     1801 cataaagact taactaatct gatgtggggc aaagttcaag caggctttta attataaaga
     1861 aaaacgctag gtcagaattg gagatgccgc attttcagcc atatttaaac tagcttcaga
     1921 tgattcttca gcgtcagagg gggctgtggc tgtttcagaa ggggagggag caacgctaag
     1981 gcgttggttt ttttctagta tcgcgagaat ttgttgtttt ttaaaatact cagccaaggg
     2041 gttcacgatg cgctccaaat aaacttaaat acattagggt cacctgatat gtacatacat
     2101 tagtgatgta atgcaacgat tttatagcgt ttatgttgta aaaaattaca gtgttgtctt
     2161 ccgcgggtca aacgcatcac gcaccgcttc accgataaaa gtcagcagtg taagcattaa
     2221 agagagggaa acgaaggctg tgattcccag ccatggggcc tgaaggttcg tttttccttg
     2281 ccggagcact tcccctaaag agggcgaacc gggcggcatg cctaggccta aaaaatcaag
     2341 cgctgtaagg gctgtaatcg ctccagtcag gataaaaggc ataaaggtta tggttgaaac
     2401 cattgcgttg ggcagcacat ggcgccacat aatcaccggg ttggacacgc ccagcgcccg
     2461 ggccgcgcgc acataatcaa aattccgcgc ccgcaggaat tctgcacgga ctagatccac
     2521 taatgaaatc caggagaaaa gcaacaggat ccaaagaaga acccagaatc caggaacaaa
     2581 tatggatgaa aaaataataa gaatgtaaag ttgcggcatg ctgctccaga cttcaatgac
     2641 gcgctgcata ataaggtcaa tcttgccgcc ataatatcct tggatagcgc ctacagcgat
     2701 tccgataatg gaactgataa gcgttagaat caggccaaat gttgaggaat tacgaaaccc
     2761 gtataaaacc cgcgccgcca cgtcgcgccc ttggtcatcg gtgccgaaaa tattctctcc
     2821 ggttggcggg gagggggccg gttggggcaa attataatta atcgtgtcgt acgaataacg
     2881 gatcggcggc cagaccatcc agcctttggc ttcaatcaat tccatgacaa acggatctcg
     2941 atattccgtt tccgtctcaa aatctccgcc aaaggtggtt tccggataat gttgaaaggt
     3001 tggaaaataa tacccaccgt cataacggat caggatcggc ttgtcattgg cgataaactc
     3061 cgcccccaga ccaatcacca aaagcgacag aaaaatccaa aagctccaaa agccacgctt
     3121 attggctttg aattgtgaca ggcgacgctg tgtgataggg gagaaggtca ttctttttcc
     3181 cccgtcattg cgaggaggcc tgcgaggccg acgcggcaat ccagactgga ttgcttcgct
     3241 gcgctcgcaa tgacgatata aaaactctgt gcctctgtgt ctctgtggtt taaattcatc
     3301 acgccgcctt cgcttcaaaa tcgatgcgcg ggtcgaccat cacatagaca atatcgcgga
     3361 tcagatgcat gaccagcgaa atcagcgcaa agatataaag agtgcccaaa acgaccggat
     3421 aatcacggtt aatcacactc tcatagccca gcaggcccaa accatccagt gagaaaatca
     3481 cctcaatcag caacgccccg gtaaagaaaa tcgacacaaa cgccgccgga aacccggcaa
     3541 tcacaataat catcgcatta cgaaacacat gcccgtacag cacctgcttt tcactcaagc
     3601 ctttagcccg cgcggtcagc acatattgct tgttaatctc atccaaaaag gaatttttcg
     3661 tcagcagcgt cagcgacgca aaacccgaaa taaccatcgc ggcaatcggc aataccatat
     3721 gccacatata atccagcgcc agctcgataa agctcatttc ctcccagccg tcggacacca
     3781 gaccgcgcaa ggggaaaagg tcgaaatacc gccctccggc aaacaccacg atcaacaaaa
     3841 tcgcaaacat gaaggacgga atcgcataac cgataaacac cgctgcgctg gtccagatat
     3901 caaacggcgt cccatcccgc accgccttct taatccccag cggaatcgaa atcagataaa
     3961 taatcagcgt actccacagc cccagcgaaa tcgacaccgg catcttttcc agcaccagat
     4021 ctaaaacgcc aatatcccgg taatagctcg tccccagatc aaaacgcgca taattcttga
     4081 gcataatcca gaaccgctca tacgctggct tgtcaaaacc atataaagct tccaattcgg
     4141 cgataaattc cgggtccagt ccctgcgctc cgcgatattt gctgccttga cccgtcgccg
     4201 tttggctcat cgcacccaca gcatcgccac cgcctgagct gccgccaaaa cgcgccgtgg
     4261 cttccatgtc atggccctgc aactgcgcga tcattttctc caccggcccg ccgggcacga
     4321 actggataat cacgaacgaa atcagcagta tcccgaacaa tgtcgggagc atcaataaca
     4381 agcgtttaaa gatatacgta gccatgctta aggtttaaca ttccagtcca gcacacgcca
     4441 gagtccatct tcatgtttca acagccccaa ggcgcctgtg gcgagcttaa tgctatgtcc
     4501 ggccgcaaag ccctcaaaat cccctgtaat atgcggggcg aagcgcgcaa tgccgttcga
     4561 ggtcacaacc aacactgttt catcgtcatc atgcgcggca atctgatcgg caaaattatg
     4621 ccagttctgg ataattgcat caggatcaac cttccacccg tcgggcacaa ggccatgctc
     4681 atcccaatcc gaaagggcct gtttgccaat ccgcgcgatc acatcggcct cgaccttatt
     4741 ctcatccggc ccgtaatcaa tttcatcaaa aataccaagc tgataaaccg gattgcttac
     4801 acctgattct tgaatagaaa tttccgccgt ttcaatcgta cgttttaaat gtgaagcata
     4861 aacaacatcc gggatcatct tgttttcttt gaggtaatgt ccaatcgctt tagcttgcgc
     4921 gcgccccttt tcgaccagcg gcaaatcggt atgccgcccc acccgtgtcg gcgtatccct
     4981 agggccgaac gtgtttccat ggcgagcgat gatcagcgtg gtcatgacaa ggccttcaac
     5041 atctcttctg cccgtgccaa atcttccggc gtgtcgattc ccgattgaat ttggccttga
     5101 tcaatatcaa cgggcacagc cgtgacctca atttcgtttt ccagcattct gagttgctcc
     5161 agcccctcaa gctgttcgta atatccttga ggcaaggcgc agaaccgctc aagaatatca
     5221 acacgaaacc catacagccc catatgctga tagacgggag agagcaaagc gctgcggtct
     5281 tctttgcgga tcgccgggat aatgtttttt gaaaaccaca aagcccgccc gtctttgaca
     5341 attgcagttg taccgctaaa cggcgttgtt ttttttgctt cgcgcagacg atcaaaatca
     5401 tcccaactca ggcggtgcac aggcgtcacc acttccgggt tgttttcaaa agctttaatc
     5461 agggcttgca aaacacttag cggtgtaaac ggcgcgtcgc cctgaagatt aaccacaaaa
     5521 tcgggcattt catcgcattg ccttagcgca gccaaaaccc gatccgaccc ggttggacaa
     5581 tcatcgggtg tcatcacgca ctccacgccc aacgcggtcg catgatcggc aatacgccga
     5641 tcttccgtag ttacaaatac atccgcattt tcaatgctgc gcgctaaatc cacgacgcgc
     5701 gaaagcatac tctgtccgcc gatctgcgcc aagggtttgc ccggaaaccg tgttgagccg
     5761 tagcgcgcag gaataacaat cgctgttttc atctactgcg cacctttccg ctttgcccac
     5821 catgtgtcag aaatgagcgg gctaatgtct gacagtggat cggggcgctc gatatgtgtc
     5881 cagcgcgcaa tccgccattt cggataatgc cacatcggaa tcacataatg attccacagc
     5941 aaaattctgt ccattgcccc gacatgcgcc accagatcct cgcggctgga agtctggatc
     6001 agctcttcaa tcaactgatc aatcaccggg tcctgaatgc cgatataatt gcgcgaacct
     6061 tcaatcgccg ccttttcgct gccccagaaa tcacgttgct cgttgcccgg cgagcttgat
     6121 tgcccaaacc cgctgatagt catatcaaaa tcaaatgcat tcatccggtt ttgatattgt
     6181 gcactgtcca ccacgcggaa attcgccttc acgccaatcc gctccagatt tttgataaac
     6241 ggtaataccc agcgctcaaa catcgggttt gcgtctagaa tttcaaactc gagcttggcg
     6301 ccgtcttttg caagcacacc gtcggtttct aaaacccatc cggcttcatt aaggagccct
     6361 gtggctttac gcagattcgc gcgcgcattg cctgacccgt cgctcacagg aggtgcatag
     6421 gtttttgtaa acacctcatc cggtattttc ccgcgaaagc gctccagcaa ttccagctct
     6481 ttcccttccg gtaatcccga agaagccagt tccgaattct caaaatagct atccgtgcgc
     6541 gtataggtgc cgtaagcaaa ttgcttgttc gaccactcaa aatcaaacgc gtaagacaaa
     6601 gcttcgcgca cggctctgtc tttgaacaca tcgcggcgga tattatagat aaacccctgc
     6661 atcccggcag ggcgcgcatt gtctatctct tccttcacaa tccgcccgtc tttcacaggc
     6721 tgtgc
//
LOCUS       DAOSWR010000111         6688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7466_length_6688_cov_4.977687, whole genome shotgun sequence.
ACCESSION   DAOSWR010000111 DAOSWR010000000
VERSION     DAOSWR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_6688_cov_4.977687"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..561
                     /locus_tag="PK513_08205"
     CDS             40..561
                     /locus_tag="PK513_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82469.1"
                     /translation="MGPPLIAIDENYRTSAAHIYALGDVANHHNLTPVAVQEGHVLAD
                     RLFNNQPERCVNYDNIATAVFSSPPIGTVGISEKDALDHGHKIDVYVSSFKPMKHQLT
                     GRDERSFLKMIVDQDSDRVLGLHMMGLDAPEIIQGFAMAINMGATKTDFDRTMAIHPT
                     SAEEFVLMRTKRD"
     gene            complement(570..1742)
                     /locus_tag="PK513_08210"
     CDS             complement(570..1742)
                     /locus_tag="PK513_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82470.1"
                     /translation="MISFFRAKISKNEHLQSLNSLRSILKDEEIGDWSRAVELTTVLD
                     DVCKAFDKDVKSLETYEECIFPDMNNATATTADTVEYLREWHVIGNAPSLPAMMAVSC
                     GVFGIESPDHVQTLLMAGVLGEVENDLPYHNNMHFKKVALQTMRMIAVHNDIFEGTAR
                     AFGPKEITMLLAAACIHDLDHDGLGNMIKGNFYQGRLERRAHEIVVPYFRATGMDEES
                     LTLLKSMLLATDVSPLGSVTNPLHQMKSAYRRHYKGEKGLHNTLHLDEELEIFELNPM
                     AAQMACLLQEADIATSGGLDYTVTQYETVQIYTEIKLYGARPSHVLDFLDKVCQRCFL
                     TDAGQRLFGANMARICALAEEDYANGDEPFPKAQHTDFILGTSANKSCKTDPSRLN"
     gene            1918..3309
                     /locus_tag="PK513_08215"
     CDS             1918..3309
                     /locus_tag="PK513_08215"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOO82471.1"
                     /translation="MTQGWNPESWRTKPIKQMPEYTDLAALEAVEGELSALPPLVFAG
                     EARSLQHSLGQAAQGQGFLLQGGDCAESFAEFSANHIRDTFRVLLQMAVVMTYGGSLP
                     VVKVGRMAGQFAKPRSSDTETQGDVTLPSYRGDNINSAEFTHEARIPDPQRLIRAYHQ
                     AASTLNLLRAFAKGGYADLNRVHGWNLDFVSDSPLKERYEDLASRIDDALRFMAACGV
                     TSETVPAIQQTDFYTSHEALHLNYEQAMTREDSLTGDWYDTSAHFLWIGARTHQEDHA
                     QIEFVRGIKNPLGLKCSPALESDELIRLIDVLNPENIPGRLTLIARMGHEQVEDKLSP
                     LLRKVKEEGRSVVWSCDPMHGNTYKADSGYKTRNFEHVLAEVRGFFAAHKAESTHPGG
                     VHFEMTGANVTECVGGAEAIGDEDLSNRYHTACDPRLNANQALELAFLIADELKAVRQ
                     SEQPQPVTAAAAE"
     gene            3311..4939
                     /locus_tag="PK513_08220"
     CDS             3311..4939
                     /locus_tag="PK513_08220"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOO82472.1"
                     /translation="MPESLTLTLAQLNPTVGDLRENAEKILDVWDESESDLVVFAEMA
                     LCGYPPEDLVLKPSFINSIHSFVESICEQSKDFKSAALISCPWRIEDEVYNAALLIEN
                     GQIVAVQPKHHLPNYGVFDEQRIFKAGPLPAPIDFRGYRLGILICEDMWYADVAAHLK
                     AEGAELLIVPNASPFETTKDETRLDIARARAGETGLPLLYVNQVGGQDELVFDGGSFV
                     MDGEGNAVFQAAEFTEETHGITFSGRRLSCPDSVPQFSPEEELYKALVLGLKDYIEKN
                     GFPGVLLGLSGGIDSALSAAIAVDALGPERVHCVMMPSPFTSEESLIDAQDCANALGC
                     PYETMPIDAAMEAFTSIIPGLSGIAHENMQSRVRGLILMALSNASGNMALSTGNKSEM
                     AVGYATLYGDMNGGFNALKDLYKMQVYALSKWRNAHKPTGALGPGGVVIPENILTKVP
                     TAELKENQRDQDSLPPYEELDDILECLIEHDMAMDEICARGHDEATVKQVWAMLDRAE
                     YKRRQAPPGVKITRRAFGRDRRYPITNKFFSNQE"
     gene            complement(4930..5472)
                     /locus_tag="PK513_08225"
     CDS             complement(4930..5472)
                     /locus_tag="PK513_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82473.1"
                     /translation="MSEQTEERIIRLGLRGMVYGQATEDQPYGSYGYDLEGEGPRRAT
                     IHVVASLADLYNQVADRDVLPAVESPSCEVDVFSARHKGDVVKRVVRDGPPVELRSFS
                     RDGDDQSGVNLFVFPKMGDENQLGIAGFNYAARLTPPIKGAGNVRLIEHFTRLNPIQT
                     QKEIEAALSSMIDIAHNAHS"
     gene            5712..>6688
                     /gene="gltX"
                     /locus_tag="PK513_08230"
     CDS             5712..>6688
                     /gene="gltX"
                     /locus_tag="PK513_08230"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOO82474.1"
                     /translation="MSVKVRFAPSPTGMLHVGNARTALMTWLFARSQGGRFLLRIDDT
                     DPERSKVEYELAIEDSLMWLGLNWDEKANQKDRLARYQEQIAKLQEAGRIYPCYETPE
                     ELGLMRKTLLSRSKPPIYDRAALSLTDAQIAKYEAEGRRPHWRFKLNHEPIEWNDHIR
                     GEVKFNGSDLSDPVVIREDGSPLYHLCSVIDDIDFDITHIIRGEDHVSNTATHIQMFE
                     ALGAVPPEFAHNALISDAEGGKLSKRLGSLSIRDLRETEGLEAMAVSSLLARLGTSDP
                     IEAFTSLEPLVESFDFSKFSRASAKFDPAEIFRLNAKIIHDTPFEAVNVR"
BASE COUNT         1683 a         1639 c         1699 g         1667 t
ORIGIN      
        1 ttcggtcata gatcgtagct taactcactg tccgaattct tggggccacc tctcattgca
       61 attgatgaga attaccgaac ctcggctgcg catatttatg ctctgggaga tgttgcgaat
      121 catcataact tgacgccagt ggccgtgcag gaaggccatg ttctggctga ccgattattt
      181 aacaaccagc cggagcgctg cgtaaattac gataatattg ccacagcggt gttttcatct
      241 ccgccgattg gcacagtggg aatcagcgag aaagatgcgc tggatcatgg ccataaaatt
      301 gatgtgtatg tctcttcgtt caagccaatg aaacaccagc ttacaggccg ggatgaacgt
      361 tcattcctta agatgattgt tgatcaggac agtgaccggg ttttaggtct gcatatgatg
      421 ggcctggatg caccggaaat cattcaaggc tttgcgatgg cgataaatat gggtgcaacc
      481 aagactgact ttgaccggac catggcgatc catccgactt cggctgaaga atttgtattg
      541 atgcggacga aacgagatta atgacaagat tagttaaggc gggagggatc agttttacag
      601 cttttattag cagacgttcc gagaataaaa tcggtatgct gcgcttttgg aaacggctcg
      661 tctccgttgg cgtaatcctc ttcagccaag gcacagattc gcgccatatt cgcaccgaag
      721 agtctttgcc cagcatccgt taaaaagcaa cgctggcaaa ccttatcaag aaaatccaga
      781 acgtggctgg ggcgcgcccc gtagagtttt atttccgtat aaatctgtac ggtttcatat
      841 tgcgtgaccg tgtaatccaa ccctccagat gttgctatat cagcttcttg cagcaaacat
      901 gccatctgcg ctgccatggg gtttaactca aaaatctcca gctcctcatc aagatgtaaa
      961 gtattatgga gtcccttttc acctttatag tgccgccgat aagctgattt catttggtgc
     1021 agcgggttgg tcacactccc taacggtgaa acatccgtcg ccagcagcat tgatttgagc
     1081 agcgttaaag attcttcatc cattccggtt gcacgaaaat acggcaccac aatctcatgg
     1141 gcgcggcgtt ccaggcgccc ctgataaaaa ttgcccttaa tcatgtttcc cagcccatcg
     1201 tgatcaagat catgaatgca ggcggcggcg agcagcatcg ttatttcttt cggaccgaac
     1261 gctctggctg tgccttcaaa aatatcatta tgcacggcaa tcatgcgcat agtctgcaac
     1321 gcgacctttt tgaaatgcat attattatgg tatggcaaat cattttcaac ctcacccaac
     1381 actccagcca tcagcaatgt ttgcacatgg tctgggcttt caatcccgaa aaccccacag
     1441 gacacggcca tcattgccgg caacgagggc gcatttccta tgacatgcca ctcgcgcaga
     1501 tactcaacag tatctgctgt cgtcgctgtt gcattattca tatcaggaaa aatacattct
     1561 tcgtatgtct cgagagattt cacatcttta tcaaacgctt tacaaacgtc gtctaggacc
     1621 gtcgtcagtt ctacggcacg ggaccaatcg ccaatctcct catcctttag tatagacctt
     1681 aaagaattaa gtgattgcaa atgttcattt tttgatattt ttgcgcgaaa aaaactgatc
     1741 atagccgtcc gatttccggt caaatttctt acctcgttac attgtacggt gttttaagac
     1801 cgttgtaaaa aataaaaata caattatacg ttgtatatca gttaggcttt gcgcaaagct
     1861 tcggtttcct ttataaattt ttgtcttata tattgtgtga tcaatggagt tttaagcatg
     1921 acacaaggtt ggaatcctga aagctggcgc acgaagccca tcaagcagat gccggaatat
     1981 acggacctgg cagctttgga ggctgtcgag ggagaactca gcgcccttcc gccactggtc
     2041 ttcgcaggag aagcgcgtag cctgcagcac agccttgggc aagcggcaca ggggcaagga
     2101 tttctgctcc aaggtggaga ttgcgccgaa agttttgccg aattcagcgc caatcatatt
     2161 cgcgatacgt tccgcgtatt gttgcaaatg gctgtggtaa tgacttatgg cggatctttg
     2221 ccggttgtta aagtcggacg aatggccggg caatttgcca aaccgcgctc cagcgatacg
     2281 gaaacgcaag gtgacgtaac tttgccgagc tatcgtggcg ataatatcaa cagcgcagaa
     2341 ttcacgcatg aagcgcgcat ccccgatccg cagcgcctca tccgcgcgta tcatcaagcg
     2401 gcatccaccc ttaatctctt gcgcgccttt gccaaaggag gctatgcgga tttaaaccgt
     2461 gtacatggct ggaatctgga ttttgtttcc gacagtccgc tcaaggaacg ctatgaagat
     2521 ttggcaagcc gtattgatga tgctttacgg tttatggcgg catgtggcgt tacatccgag
     2581 accgtcccag ccattcaaca gacagatttt tatacttctc atgaagcgct gcatctgaat
     2641 tacgaacaag cgatgacccg cgaagacagc ctgaccggag actggtatga tacgagcgca
     2701 cattttctgt ggatcggtgc ccggacgcat caggaagatc atgcacagat tgaatttgta
     2761 cgcgggatta aaaatccgct gggcctaaaa tgttctccag cattagagtc cgatgaactc
     2821 atacgcctga tagatgtctt aaaccctgag aatattcccg gccgcctgac cttgattgca
     2881 cggatgggcc atgagcaggt tgaggacaaa ctttctccgt tgctgcgtaa agtcaaagaa
     2941 gagggacgca gcgttgtatg gtcttgtgat ccaatgcatg gcaataccta caaggctgat
     3001 tcaggttaca aaacccggaa cttcgaacat gtcctggccg aggttcgtgg attttttgcc
     3061 gcgcataaag ccgaaagcac gcatccaggc ggcgtacatt ttgaaatgac cggcgccaat
     3121 gtaacagaat gtgtcggcgg cgccgaggcg attggcgatg aagatctttc gaaccgttat
     3181 cacacagcgt gtgacccccg cctgaacgct aatcaagctc tggagcttgc ctttttgatt
     3241 gccgatgaac ttaaggctgt acgtcagagc gaacaaccgc aacctgttac ggcggcggct
     3301 gccgagtaag atgcctgaga gcctcacact cacccttgct cagcttaatc caaccgtagg
     3361 agatctgcgc gaaaatgcag agaaaatact tgatgtatgg gacgagtctg aaagcgatct
     3421 agtggtgttt gccgagatgg cgttgtgcgg atatccgcct gaggatctcg ttttaaaacc
     3481 gagctttatc aattccatac attcttttgt tgaatcaatt tgtgagcaaa gcaaagactt
     3541 taaatcggca gcgctgattt cctgcccgtg gcgcattgag gatgaggttt ataatgcggc
     3601 gctgctgatt gaaaatggtc agatcgttgc tgttcaaccc aagcatcatt tacccaatta
     3661 cggagtgttt gacgagcaac gcattttcaa ggccgggccc ctgcccgcac cgattgactt
     3721 tcgcgggtat aggctgggca tcctgatttg cgaagatatg tggtatgcgg atgtggcggc
     3781 gcatcttaag gccgaaggcg cagagctttt aattgtgccc aatgccagcc cgtttgaaac
     3841 aaccaaagat gaaacgcgcc ttgatattgc ccgcgcgcgc gccggtgaaa ccggtctacc
     3901 cctgctttac gtcaatcagg tcggcgggca ggatgagttg gtgtttgacg gcggttcgtt
     3961 tgtgatggat ggtgaaggca acgccgtgtt tcaggccgct gaatttaccg aagaaaccca
     4021 tgggattact ttttccggta gaagattgtc ttgcccagac agcgtgccgc aattcagccc
     4081 ggaagaagag ctgtataaag ccttggtgtt ggggcttaaa gactatattg aaaagaacgg
     4141 gtttccgggc gtgctgctgg gcctatcggg tgggattgac agcgcgcttt cggcggcgat
     4201 tgcggtcgat gcgctggggc cggagcgcgt acattgcgtg atgatgccct ccccgttcac
     4261 ctctgaggaa agcctgattg atgcacaaga ctgcgccaat gctttgggat gcccttatga
     4321 aaccatgccg attgacgcgg ccatggaagc gtttaccagc attattccgg gcctgtcagg
     4381 cattgcgcat gaaaatatgc agagccgggt gcgcgggctg atcctgatgg cactgtccaa
     4441 tgcatcgggc aatatggcgc tgtcgacggg caataagagc gagatggcgg tcgggtatgc
     4501 aacgctgtat ggcgacatga atggcgggtt taacgccctg aaagacctct ataagatgca
     4561 agtgtacgcg ctttcgaaat ggcgaaatgc gcataaacca accggtgcac tggggccggg
     4621 cggcgttgtc attcctgaaa atattctgac caaggttcca acggcagagc tcaaagaaaa
     4681 ccagagagat caggacagcc tgccgcccta tgaagagctc gatgatattc ttgaatgctt
     4741 gatcgaacat gatatggcca tggatgagat ttgcgcgcgc ggacatgatg aagccaccgt
     4801 taagcaggtt tgggcgatgt tagacagagc ggaatataaa cgccgccaag ctccgccggg
     4861 agtcaaaatc acgcgccggg cctttggccg tgaccggcgc tatccgatta cgaataaatt
     4921 cttttccaat caggaatgag cgttgtgggc aatgtcgatc atagaagaca acgcagcctc
     4981 aatttccttc tgcgtttgta taggattaag tcttgtaaaa tgctcaatca atctaacgtt
     5041 tcctgcccct tttatgggag gagtcaaacg agcggcataa ttaaagcctg cgatgcccag
     5101 ttggttctca tcgcccattt tcggaaaaac aaacaaattt acacctgact gatcgtcgcc
     5161 gtcacgcgag aaagacctca actctaccgg agggccatcc ctgacaactc ttttaacaac
     5221 gtccccttta tgtcttgcac taaacacatc gacttcacaa ctggggcttt ccacagcagg
     5281 aagaacatcc ctatctgcta cttggttata caaatccgcc agagaggcga ccacatgaat
     5341 agtggcacga cgtgggcctt caccttctaa atcataaccg taggaaccat agggctgatc
     5401 ttctgttgcc tgaccataga ccattcccct tagccctagc cgaataattc tttcttccgt
     5461 ttgttctgac atttttattc accttatgtt acgcattaaa cgctatgaca aataaagacg
     5521 cgaaggcatt ctgtcaacag gggaataaaa aacacagcgc gcacagcgaa gacacagcgc
     5581 gcttttcctt gtatttaaat tccaaaaata ataaaagaat gcggataagg ttttgttttt
     5641 taaactgtaa attcaaagac catctctgaa cgcgctgtga ttgctgtgtt tttataaaaa
     5701 gaaagaaaaa gatgtctgta aaagttcgtt ttgccccttc ccccaccggt atgctgcatg
     5761 ttgggaatgc acgcacagcc ctgatgacat ggctgtttgc ccgttcgcaa ggcggacggt
     5821 tcctgctgcg cattgatgat acggacccgg agcgttcaaa ggtcgaatat gagctggcga
     5881 ttgaagattc cctgatgtgg ctggggctga actgggatga aaaggccaac cagaaggacc
     5941 gtctggcgcg ctatcaggaa cagattgcca aactgcagga agccgggcgc atttatccgt
     6001 gctatgaaac gccggaagag ctgggcttga tgcgcaagac cttgctcagc cggagcaagc
     6061 cgccgattta cgaccgggcg gcgctctccc tcaccgatgc acaaatcgcc aaatatgaag
     6121 ccgaagggcg acgcccgcac tggcggttca agctcaatca tgagccgatt gaatggaacg
     6181 accatattcg cggcgaagta aagtttaacg gcagtgattt gtccgaccct gtcgtgatcc
     6241 gcgaggatgg cagcccgctt tatcatctat gctcggtgat tgacgatatt gatttcgaca
     6301 ttacgcatat cattcgcggg gaggatcatg tctctaatac ggcgacgcat atccaaatgt
     6361 ttgaagcgct gggcgcagtt ccgccggaat ttgcccataa tgccctgatt tccgatgccg
     6421 aaggcggcaa gctgtccaag cgcctgggct ctttgagcat tcgggatttg cgcgaaacag
     6481 aagggctgga ggcgatggcg gtgagcagcc tactcgcccg tctgggcacg tccgatccga
     6541 ttgaagcctt taccagtctt gagccgcttg tcgagagttt tgatttttcc aaattctccc
     6601 gcgccagtgc aaaatttgac ccggccgaaa ttttccgtct caacgccaaa attattcacg
     6661 acacgccgtt tgaggctgtg aatgtgcg
//
LOCUS       DAOSWR010000112         6538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7712_length_6538_cov_5.327163, whole genome shotgun sequence.
ACCESSION   DAOSWR010000112 DAOSWR010000000
VERSION     DAOSWR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_6538_cov_5.327163"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1344)
                     /gene="ispD"
                     /locus_tag="PK513_08235"
     CDS             complement(133..1344)
                     /gene="ispD"
                     /locus_tag="PK513_08235"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOO82475.1"
                     /translation="MPSKVLQYNLNDLPAFHVLIASAGTGSRLGTAATPKQYLKIHNK
                     AVLRYSIETFLSMPTCASLQVIIGADDAELYHDAVRGLELPKPIIGSDKRNKSIFNGL
                     KEIHKVKNEDMILIHDAARPCIATDDIATLLMSLQTERAASLATPVSATLRRAGDGNK
                     AAETVPRDNLWAVQTPQGFLFGDLLKAHETANPHTNATDDTALVSAIGIPVTLVEGSA
                     SNIKITYPQDLIMAEKILATQTVPLTGLGFDVHAFDKNQCGPVRIGGIDIDHDHALKG
                     HSDADVALHALTDAILGTIGEGDIGQHFSPSDDAFKNMDSSLFLKKALELMHAQSAHL
                     NNIDLTIICEAPKIGPHAGPMRARLAKLTGLPENRINIKATTSEGLGFTGRREGVAAQ
                     AIVSVSAKEAA"
     gene            1383..3635
                     /locus_tag="PK513_08240"
     CDS             1383..3635
                     /locus_tag="PK513_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOO82476.1"
                     /translation="MNLSMFASVYKRFKDLTAVKSRNTQNAAVIGLVIASIISGIATY
                     GALTKTPPFGNDPKTVIWLLNLDLVLLLVLVGVVAQRLVGVWSGRKRGLAGSHLHVRL
                     VYIFSVLAAAPVIIMTVFSAVFFYFGVHTWFSERVSTAVNESQAVAEAYLREHTQTIR
                     ADTLAMANDLNREARLLLLNEQAFDQVMQTHSLLRDLSEAIIFDDSGRVLARSGLTFS
                     LEFEDIPLYAMEGADNGEVVIMTGGNEDRVRALVKLDNFVDAYLFVGRMIDPQVLSHL
                     AATKQATHDYAILQERLSDLQVTLGLIFVVVGLLLLMSAIWFGLVLARQLVQPISTLI
                     TTADSVRAGDLSAQVPPIKGIKEFDYLAQSFNRMTRRLQEQQNELLQANRQLDRRRHF
                     TETVLTGVSSGVIALDEKGLISVANASACELLGVEIEALSERDIGAIMPEISNLLDEA
                     HRQPEKITQGEIPFLRRDELRRTFLVRVAIERIGDQETGIILTFDDITELQSAQRKAA
                     WADVARRIAHEIKNPLTPIQLSAERLNRRYLKQIKDDPETFTRCTETIIRHVGDIGRM
                     VNEFSSFARMPEPVMHIEDLQEQIRDVVSLHKQAHCDVKIECADIAPGLIGNLDAQQI
                     RQAINNLVQNALDSIRGQKEQAQVGRIHLLAGRHEDNIFVAVSDNGPGFPEDQERASL
                     TEPYVTHKQKGTGLGLAIVKKIMEDHNGRIILGAPQWLQDYEGWQGLDGATAVLVLRS
                     VQSAESEEAA"
     gene            3651..5108
                     /locus_tag="PK513_08245"
     CDS             3651..5108
                     /locus_tag="PK513_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO82477.1"
                     /translation="MSSDILIVDDEADIRSLVRGILEDEDYTVREAASCEQAYAAVAK
                     KIPALVVLDIWLQGSKDDGLGILRKLKADHPGLPFVMISGHGTIETAVSAIKDGAYDF
                     IEKPFKSDRLLMMIARALETAHLRRENASLKKYNFGQADQLIGKSVAVQKIQQLVERV
                     APTNSRILIQGEAGTGKDMIARLIHSRSQRANEAFMALNCATLRPERLETELFGSEAG
                     VNGEGEHIGILEQSHGGTLVLDEVADMPLETQGKIVHTLQEQSFQRIGGQTTIETDVR
                     ILATTNRDLEAAIKAGAFREDLYYRLNVVQIEAPALRERKEDIAVLAEGLLQAQLRLL
                     GREERHFSSAALNALKAYDWPGNVRQLKNVIEWVCIMHSGQGSEMLGVDQLPPEVTGK
                     HVAVDGVNFSGGGGALEDDYLELSLREAREAFEKEYLLAQVARFDGNISKTAHFIGME
                     RSALHRKLKSLQIHSDAPQGQDGPLADPAVNRKRA"
     gene            5113..6489
                     /gene="trkA"
                     /locus_tag="PK513_08250"
     CDS             5113..6489
                     /gene="trkA"
                     /locus_tag="PK513_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOO82478.1"
                     /translation="MRAIICGAGQVGYNIAEYLSKEENDVTVVDLRRDLIAQINDELD
                     VNAVLGHASNPDVLKMAGANDADMIVAVTQSDEVNMVACQIGHSLFGIPKKIARVREQ
                     VYLQPEWSNLFSRAHMPIDVIISPEVLVAKDIYQRLAVPGTTFVNIIADGLAHMIGVI
                     CEEDCPVINTPLNQLHNLFPDLSFQVLAILRKNKPIIPDATDQLEVGDEVFFIVDTQH
                     MKRVMTAFGHEEDQARKIIIAGGGNIGTTLVDLIYTRSKNVQIKVIEQNQSRAEYLSE
                     RLENTVILNGSSLDKDILNEASIQSAETLVAVTNDDESNILGSLLAKQYGCERVITLV
                     NNEAYYPLVGPLGIDAMVSPRSIIVANIMQHVRRGRVKGLYNIRDGFAEVIEAEVSEN
                     SSIVNSTVEELNLPHEVIVGGVIREGEFIMPELSFTVRAGDDLIILASRTQAQNVEKM
                     FSVQVDLF"
BASE COUNT         1535 a         1242 c         1994 g         1767 t
ORIGIN      
        1 atagaatgat gccaaagggc agggcaccga gagagcccca ggtgcctggg gcgggtttca
       61 tgaaaccgca gccgaaccat gtggccagcc agatataggg ggcttttagg ttaagcttgg
      121 gaggtttgtt tgtcatgcgg cctcttttgc ggaaacagag acgatggctt gggcggcgac
      181 gccttcgcgc ctgccggtaa agccaagccc ttcactggtg gtggctttga tgttgatacg
      241 attttccgga aggccggtta gtttggccaa acgggcccgc atggggcccg catggggccc
      301 gattttgggg gcttcgcaga tgatggtgag atcgatattg ttgaggtgtg cgctttgagc
      361 gtgcattagc tccaatgctt ttttaaggaa aaggctgctg tccatgtttt taaatgcatc
      421 atcagatggc gagaaatgct ggccaatatc gccttcaccg attgtgccga ggatcgcgtc
      481 tgtcagagcg tggagcgcga catcggcatc ggagtggcct ttgagcgcat ggtcgtggtc
      541 aatatcgatg ccgccgatgc gcacggggcc gcattgattt ttgtcaaagg cgtgaacgtc
      601 aaagccaaga ccagtgagag gcacggtttg agtggcaaga attttttcgg ccatgatgag
      661 atcctgcggg taggtgattt tgatgttgct ggcgctgcct tcgaccagtg ttaccgggat
      721 tccgatggca ctgacaaggg cggtgtcatc ggttgcattt gtgtgcggat tggcggtttc
      781 atgcgctttg agtaaatcac caaagaggaa gccttgcggg gtttgcacgg cccagaggtt
      841 gtcacgtgga acggtttctg cagctttgtt cccatcacca gcgcgtctta gtgtagcact
      901 gacaggcgtt gcgaggctgg cggcccgttc ggtttgcaga ctcatgagaa gtgtggcgat
      961 atcatcagtg gcaatgcagg ggcgggcagc atcgtggatc aggatcatat cttcattttt
     1021 tactttatgt atttctttta atccattgaa tatactttta tttcttttat ctgatccgat
     1081 gattggtttg ggtagttcca gtccgcgcac tgcgtcatga tacaattctg cgtcatctgc
     1141 gccaataatg acttgcagtg aagcgcaggt tggcatggat agaaatgttt caatactata
     1201 gcgcagtaca gctttattat ggattttgag atattgtttt ggtgtggcag cggtgcccag
     1261 ccgtgaacct gttccggctg aggctatgag cacatgaaaa gcgggaagat cattgaggtt
     1321 gtattgtaag actttcgagg gcaaaagctt caaaatcctt gattagcttg ggattgacgt
     1381 atatgaattt atccatgttt gcatccgtgt acaagcgttt taaagatctc acagcggtta
     1441 aatcccgaaa tacccaaaat gctgcggtga ttgggctcgt gatcgcttcg attattagcg
     1501 ggattgcaac ttatggcgcg ttgacgaaaa cgccaccctt tgggaatgat ccaaaaactg
     1561 taatttggtt acttaatctc gatttggtct tgttgctggt tttagtcggg gtggtggccc
     1621 agcggctcgt tggtgtgtgg agtggccgta agcgcgggtt ggcggggtcg catctgcatg
     1681 tgcggttggt ttatattttt agtgtgctgg ctgcagcgcc ggtgattatt atgaccgtgt
     1741 tttcagctgt gttcttttat ttcggagtgc atacatggtt cagtgagcgg gtgagtacgg
     1801 cagtgaatga gtcgcaagcg gtcgccgagg cctatttgcg tgaacatacc cagacgatcc
     1861 gtgcggacac tttggcgatg gctaatgatt taaaccgcga ggcgcggtta ttgcttctta
     1921 acgagcaggc ttttgatcaa gtgatgcaga cgcattcctt gctgcgggat ttgtctgagg
     1981 cgattatttt tgatgatagt ggccgggtgt tggcccgcag cgggctgaca ttctcattgg
     2041 aatttgagga tataccgctt tacgctatgg aaggggcgga taacggcgaa gtggtgatta
     2101 tgaccggcgg taacgaagac cgggtgcgcg cgctggtgaa gctggataat tttgtcgatg
     2161 cgtatttgtt tgttggtcgg atgattgacc cgcaagtgct ctcacatctg gcggcaacga
     2221 agcaggcaac gcacgattat gccatattgc aggaacggct ttcggatttg caggtgactc
     2281 tggggttgat ttttgtagtc gtcggattgt tgttattgat gagtgcgatc tggtttgggt
     2341 tggtgctggc gcggcaattg gtgcagccga tcagtacatt gatcaccaca gccgatagtg
     2401 tgcgcgccgg ggatttatcg gcgcaagtgc ctccaattaa aggcattaag gagttcgact
     2461 atctggcgca gagttttaac cgtatgacgc ggcggctgca ggaacaacaa aacgagttat
     2521 tgcaggccaa ccgccagctt gatcgacgca ggcattttac cgagacagtg ttgaccgggg
     2581 tttcgtcggg ggtgatagcc ctggatgaaa agggtttgat cagtgtggcg aatgcttcgg
     2641 cttgtgagtt gttgggggtg gagattgagg cgttgagcga acgggatatt ggtgcgatta
     2701 tgcctgagat ttctaatctt ttggatgagg cgcaccggca gccggagaaa attacgcaag
     2761 gggagattcc attcttgcgc cgcgatgagt tgcggcgaac gtttttagtg cgcgtagcaa
     2821 ttgagcgaat aggggatcag gaaaccggaa ttattttgac ttttgatgat attaccgaac
     2881 tgcagtcggc gcagcgcaag gcggcgtggg cggatgtggc gcggcgaatt gcgcatgaaa
     2941 tcaaaaatcc gctgacgcct attcagcttt cagctgagcg gcttaacagg cgttatttga
     3001 agcagataaa agatgatccg gagactttca cacgctgcac ggaaacgatt attcgccacg
     3061 ttggggatat tggacgaatg gtcaatgagt tttcatcgtt tgcgcggatg ccggagccgg
     3121 tgatgcatat cgaggatctt caggagcaaa ttcgtgatgt tgtgagtttg cacaagcagg
     3181 cgcattgtga tgttaaaatt gaatgtgctg atatagcacc gggtttgatc ggtaatctgg
     3241 atgcacagca aattcgtcaa gcaattaata atctggtgca aaacgcgctg gattcgattc
     3301 gcgggcagaa ggagcaggcg caagtcggac gcatacacct tctggccggg cggcatgagg
     3361 ataatatttt tgtggcggtg agcgataatg ggccgggttt tcctgaagac caagagcgtg
     3421 ccagcctgac cgagccatat gttacgcaca aacaaaaggg aaccgggctg gggttggcta
     3481 ttgtgaaaaa gattatggaa gatcacaatg ggcgtattat tttaggtgcg ccgcaatggt
     3541 tgcaagatta tgaaggttgg caagggcttg acggcgccac ggcggtactg gtgttacggt
     3601 cggtgcagag cgctgaaagt gaagaggccg cgtaaggaaa tggggcgtaa atgagttcgg
     3661 atattcttat tgttgatgat gaagccgata ttcggagctt ggtccggggg attttggagg
     3721 atgaggacta tacggtgcgt gaagcggcga gctgtgagca ggcatatgcc gcggtagcaa
     3781 aaaaaattcc agctttggtc gtgcttgata tctggttgca gggaagtaag gatgacggac
     3841 tgggaatttt gcgcaaattg aaggcagatc atccaggatt accttttgtg atgattagcg
     3901 ggcatggcac gattgaaacg gctgtgagcg ccattaagga cggtgcttat gattttatcg
     3961 aaaagccgtt taagtctgat cgtttattga tgatgattgc gcgcgcattg gaaaccgcgc
     4021 atcttcgtcg ggaaaacgcc agtttgaaaa aatacaattt cgggcaagcg gatcagttga
     4081 ttggtaaatc tgtagcagtg caaaagattc aacagcttgt tgagcgggtg gcaccgacga
     4141 atagccgcat tttaattcaa ggcgaagcgg gtacggggaa ggatatgatt gcgcggttaa
     4201 ttcacagtcg ttcgcagcgg gcgaatgagg catttatggc gctaaattgt gcgacgttgc
     4261 gccctgagcg cctcgagact gagttgtttg ggagtgaggc tggtgttaat ggagagggag
     4321 agcatattgg cattttagaa caatcgcatg gcgggacgct ggtgttggat gaggtggcgg
     4381 acatgccgtt ggagactcaa ggtaagattg tacatacgtt gcaggagcaa agctttcaac
     4441 gtatcggtgg tcagacaacg attgagacag atgtgcgaat tttggcaaca acaaatcgtg
     4501 atctggaagc ggcgattaag gcgggtgcgt ttcgggaaga tctgtattat cggcttaatg
     4561 ttgtgcaaat tgaagccccg gcgcttcgtg aacgcaaaga agatatcgca gtgcttgccg
     4621 aggggctgtt gcaggcgcaa ttacgacttt tgggtcgtga ggagcgtcac ttctccagcg
     4681 cggcgctgaa cgctctcaag gcttatgatt ggccgggaaa tgtgcgtcaa cttaaaaatg
     4741 tcattgagtg ggtgtgcatt atgcatagcg ggcaaggtag tgaaatgctg ggggtagatc
     4801 aattgccgcc tgaggtgacc ggtaagcatg tagcggtgga tggcgtaaac ttttctggcg
     4861 gcggtggtgc gcttgaagac gattatcttg aattgagttt acgagaagca cgcgaggctt
     4921 ttgagaaaga atatctgctc gcgcaggtgg cgcgctttga tggcaatatt tctaaaactg
     4981 cccactttat agggatggag cgatcggcct tgcatcgtaa acttaaatca ttacaaatac
     5041 attctgatgc accacagggg caggatggtc cgttagcgga tcctgccgta aacagaaagc
     5101 gagcatagtg caatgagagc cattatttgc ggcgccggac aagtcggcta taacattgcc
     5161 gaatatcttt ccaaggaaga aaatgacgtt acggttgttg atttgcggcg tgatttgatt
     5221 gcgcagatta atgatgaact ggatgtaaat gcagttttgg ggcatgcctc gaacccggat
     5281 gttttgaaaa tggcaggcgc aaatgatgcg gatatgattg tggcggtgac gcaaagcgat
     5341 gaggtcaata tggtggcctg tcagattggg cattccctgt ttggtattcc caaaaagatt
     5401 gcgcgcgtac gggagcaggt ttatttgcag cccgaatggt caaatttgtt cagccgtgcg
     5461 catatgccta ttgatgtgat tatttctccg gaagtgctgg tggccaagga tatttatcag
     5521 cgtttggccg taccgggcac gacgtttgtg aatattattg ccgatggatt ggcgcatatg
     5581 atcggggtga tttgtgaaga agattgtccg gtgatcaata cgccgctgaa ccagcttcat
     5641 aatttgtttc cggacctttc gtttcaggtt ttagcaattc tacgcaagaa taagcctatt
     5701 attccagacg cgacggacca gcttgaagtt ggtgatgagg tgtttttcat tgttgataca
     5761 cagcatatga agcgcgtgat gacagctttt ggacatgaag aagatcaggc tagaaaaatt
     5821 attattgctg gcggcgggaa tatcggcacg acgttggttg atttgattta tacacgttca
     5881 aaaaatgtgc agataaaagt tattgagcaa aaccagagcc gggcagagta tttaagtgaa
     5941 cggcttgaaa atacggttat tttaaacgga agttctttgg ataaggatat tctgaatgaa
     6001 gcgtctatcc agagtgctga aacattggtg gcggttacca atgatgatga aagtaatatc
     6061 cttgggtcat tgcttgccaa acaatatggc tgtgagcgcg taattaccct agtgaataat
     6121 gaggcttact atccgttggt tgggccgctt gggattgatg ctatggtcag tccgcgctca
     6181 atcattgtgg ccaatatcat gcaacatgta cgccggggac gggttaaagg gctttacaat
     6241 attcgcgatg gatttgcaga agttattgag gctgaggttt ctgagaattc ttcgattgtg
     6301 aattctactg ttgaagagct taatttgcca catgaggtga ttgttggcgg ggtaatccgt
     6361 gaaggcgaat ttattatgcc tgagttgagt tttactgttc gagcgggaga tgatttgatt
     6421 attctggctt cgcggacgca ggcacagaat gttgaaaaga tgttctcggt gcaggtggac
     6481 ctattttaag ctctatgacc gcgcgttcta tgaaattggg aatattcgat tccggttt
//
LOCUS       DAOSWR010000113         6537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7716_length_6537_cov_5.643783, whole genome shotgun sequence.
ACCESSION   DAOSWR010000113 DAOSWR010000000
VERSION     DAOSWR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7716_length_6537_cov_5.643783"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK513_08255"
     CDS             complement(<1..839)
                     /locus_tag="PK513_08255"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOO82479.1"
                     /translation="MRRVVVTGLGMVSPLGVGVDHNWNEITSGKSGIKKIEHFDVSDI
                     TSKIAGIIPRTDSENPADGAFNPDLFVEPKEQRKIDDFITYGIAAAKEAIENSGWKPE
                     GEEDQNRTGVLIGSGIGGLETIYQTSTLMNDRGPRRISPFFVPAALINLISGHVSIKY
                     GFRGPNHSVVTACATGTHAIGDAARLIALDDADVMVAGGAEGAVCRLGVAGFAAARAL
                     TTSYNDRPEEASRPWDQDRDGFVIGEGAGIVVLEEYEHAKRRGATIYGEIIGYGLSGD
                     AHH"
     gene            complement(936..1175)
                     /locus_tag="PK513_08260"
     CDS             complement(936..1175)
                     /locus_tag="PK513_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO82480.1"
                     /translation="MSDIAERVKKIVVEHLGVDADKVSEGASFIDDLGADSLDTVELV
                     MAFEEEFSVEIPDDAAEKIQTVGDAVSFIKENAAE"
     gene            complement(1308..1973)
                     /locus_tag="PK513_08265"
     CDS             complement(1308..1973)
                     /locus_tag="PK513_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOO82481.1"
                     /translation="MHSLIIFDCDGTLVDSERLYNTVTAELLNEIGYDEYTPQLCLER
                     FSGKSWSTIRDELEEHHGEPMPADIVQRYIKIAGSRMDTDLKAARHASAVLKNLKAQT
                     QICIGSNGERGNVIKSLAVTSLLSYFEEDRIFTKIQVERPKPAPDIFLFACDKMQTPP
                     ANALVIEDSPAGVNAARAAGIDVLGFIGTAHDKAAQGQALKDAGANAIIDELIHIEHH
                     LKR"
     gene            complement(1979..2716)
                     /gene="fabG"
                     /locus_tag="PK513_08270"
     CDS             complement(1979..2716)
                     /gene="fabG"
                     /locus_tag="PK513_08270"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOO82482.1"
                     /translation="MFDLTGKTALVTGATGGIGGAIAKALHSAGATVGISGRNTEKLN
                     TLATELGKRVFVLPADLSQENAIADLVKAADEALGQVDILVNNAGLTRDGLSMRMSDE
                     DWQTVIDVNMTATFKLAKALQRGMMKRRHGRIISIASVVGVTGNPGQCNYVASKAGMI
                     GWSKAMAAEVASRGITVNCIAPGFIATAMTDALNDDQKEKISANIPAGKMGTSEDIAA
                     AALYLASDEAGYVTGQTIHVNGGMAMI"
     gene            complement(2824..3768)
                     /gene="fabD"
                     /locus_tag="PK513_08275"
     CDS             complement(2824..3768)
                     /gene="fabD"
                     /locus_tag="PK513_08275"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOO82483.1"
                     /translation="MTRAFIFPGQGSQSVGMGADLYATFPEAREVFEEIDESLGQNLS
                     KLMFEGPEEDLNLTENTQPALMAVSMAVVQVLKKQGGIDFKTAARFVAGHSLGEYAAL
                     TAAESFTIADAARLLKLRGQAMQKAVPVGIGSMAAIIGPSFEDVQAIAQEAAGSDICA
                     AANDNSVGQVVVSGHKDAVARAVELATARGAKKAVTLPVSAPFHCALMQPAADAMAEA
                     LAATDIKAPIVPVVANVTAAATSDPDEIRGLLVEQVTGTVRWRESVLWMKDQGVDEMI
                     ELGAGKVLCGLVRRIDRDISCANTGTPEEIETIINSLK"
     gene            4056..4349
                     /locus_tag="PK513_08280"
     CDS             4056..4349
                     /locus_tag="PK513_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82484.1"
                     /translation="MTQNIHTRIKHLLEETNDAFASSGTINMDYAGFAAMALSDFKNL
                     LGNPDLTDMELRRVIRSGEKKRRLKDPNGCWASFIAHYVARNANQNLKEQCTL"
     gene            4528..4893
                     /gene="rpsF"
                     /locus_tag="PK513_08285"
     CDS             4528..4893
                     /gene="rpsF"
                     /locus_tag="PK513_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009805007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOO82485.1"
                     /translation="MPTYETVFMTRQDLTEGQVKDLTDKYAKVITDQGGKILKTEQWG
                     LRTLAYKINKARKANYTLVEFEAPGAAVIELERLMRIDEDVMRCLSLRLDAPTEGPSV
                     MMEKNRDRDDDTTMKKEAA"
     gene            4893..5177
                     /gene="rpsR"
                     /locus_tag="PK513_08290"
     CDS             4893..5177
                     /gene="rpsR"
                     /locus_tag="PK513_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOO82486.1"
                     /translation="MSAADVPTVKRPFFRRKKTCPFTGPYAPAIDWKDTKTLSRYISE
                     RGKIIPSRITAVSQKKQRELAKAIKRARFMGLLPYVQTETEQRQARPPRS"
     gene            5198..5971
                     /gene="rplI"
                     /locus_tag="PK513_08295"
     CDS             5198..5971
                     /gene="rplI"
                     /locus_tag="PK513_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOO82487.1"
                     /translation="MSTQVILLERVEKLGNMGDVVSVKPGYARNYLLPQKKALRASKD
                     NVAYFEAQKKHLQAESDKQKKEAEKRAKKLAGTKVALIRQASESGQLYGSVASRDIAA
                     ALTEATGESIDRSMVKLNQNYKLIGLFPIDVTLHPEVTAEITINIARSPEEAEIQAKT
                     GKALVAEGHGDEPSEDERAAAADEVLEDALEEGALEAEKERQAEAEEKAAEDAAKAEE
                     RATKKAEKDAAKAAAAEAEEAAAEAESGEDTPAEEESEA"
     gene            6303..>6537
                     /locus_tag="PK513_08300"
     CDS             6303..>6537
                     /locus_tag="PK513_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOO82488.1"
                     /translation="MVHDLAFQALEGVSHVSGDATPNYRILPHNLDAEQGLLGALLVD
                     NRAVEKMGDFLKPAHFFMPAHQRIFETILTLIER"
BASE COUNT         1574 a         1666 c         1700 g         1597 t
ORIGIN      
        1 atgtggtggg catcgccgga caggccatag ccgataattt caccgtaaat ggtcgcgccg
       61 cgccgcttcg catgctcata ttcttccagc acgacgatcc cggcgccttc gccaatcaca
      121 aaaccatcgc ggtcttggtc ccacgggcgg gaagcttctt cagggcgatc attatagcta
      181 gttgtcaagg cacgcgcggc ggcaaagcca gccacaccaa ggcggcagac agccccttcc
      241 gcgccgccag caaccataac gtcggcatca tccagcgcga ttaagcgcgc agcgtcgcca
      301 atagcatggg ttccggttgc gcaggccgtg acgacggaat ggttgggacc gcggaaaccg
      361 tatttaatag agacatggcc agagatcaga ttaatcaggg cagcagggac aaaaaacggc
      421 gaaatgcgcc gggggccgcg atcattcatt agggtagaag tctggtaaat cgtttccagc
      481 ccgccaatac cagagcctat caacacaccc gtgcgattct gatcttcctc gccttcgggc
      541 ttccagccgg aattttcaat agcttcttta gcggcggcga tgccataggt aataaaatca
      601 tcgatttttc gctgttcttt cggttcgacg aacagatccg ggttaaatgc gccatcggct
      661 ggattttcac tgtctgtgcg tggaatgatt ccagcgattt tcgatgtaat atcagataca
      721 tcgaaatgct cgatcttttt aatgccgctc ttgccggaag tgatttcatt ccagttatgg
      781 tctacgccca cgcccagcgg tgaaaccata cccaatccgg tgacaacgac tcttctcata
      841 ctacttacct tttaaatgta aagacagccg cacaaacggc ggctggcatt aaaattatgt
      901 gcaataaatt gcacctcaag tgcttttaaa acagcttatt cagcagcatt ttctttgatg
      961 aagcttacag catcgccgac tgtttgaatt ttttcggcgg cgtcatcagg aatttccacg
     1021 ctgaattctt cttcaaaagc cataaccaac tcaaccgtat ccagtgagtc tgcgcccaaa
     1081 tcatcaataa agctcgcgcc ttctgagacc ttgtcagcat ctacgcccag gtgctcgact
     1141 acaattttct ttacgcgctc tgcaatgtca ctcatcgttc ttccttgtcc ttaattagaa
     1201 atcgttttcg tctttgtaat ttaataggtc cgaactttac cagcccattt cccgattgca
     1261 agacattttg tctgattagc ataagggcat ggaatgccaa atcattttca acgtttgagg
     1321 tgatgctcaa tgtggataag ctcgtcgatt attgcatttg ccccggcatc ttttaacgcc
     1381 tgaccctgcg cagccttatc atgggccgtg ccgataaaac ccaatacatc aattccggca
     1441 gcgcgcgctg cgtttacccc tgcggggctg tcttcgatca ccagcgcgtt tgcgggcgga
     1501 gtttgcattt tatcgcaagc aaagaggaaa atgtccggcg caggtttcgg gcgctcgacc
     1561 tggattttgg tgaaaatccg gtcttcctcg aaatatgaca aaaggctggt gacagcaagg
     1621 ctttttataa cattgccgcg ttcaccgttg gagccaatac aaatttgggt ttgggctttc
     1681 aggtttttta agaccgcact ggcatggcgg gcggctttca ggtccgtatc catgcgcgat
     1741 ccggcgattt tgatatagcg ctggacgatg tctgcgggca tcggctcgcc gtggtgttct
     1801 tcaagttcat cgcggatggt ggaccagctt ttgcctgaaa aacgctcgag acagagctgc
     1861 ggggtgtatt catcatagcc gatttcattg agcagctcgg cagtgacggt gttatagagc
     1921 ctctcgctgt cgaccagcgt cccgtcgcaa tcgaagatga tgagggaatg catacccact
     1981 aaatcatcgc catgccgcca ttgacgtgga tcgtctggcc ggtgacgtaa ccggcttcat
     2041 cggaagccag atagagcgca gcggcggcaa tgtcctcgga cgtgcccatt ttcccggcgg
     2101 gaatattggc gctgattttt tctttttgat catcgttgag cgcatcggtc atggccgtgg
     2161 cgataaagcc cggcgcgatg cagttgacgg tgatgccgcg cgacgcaact tcggcggcca
     2221 tagccttgga ccagccgatc atcccggcct tggaggcgac atagttgcac tggcccggat
     2281 ttcccgttac gccgacaacg gaagcgatac taatgatgcg gccatggcgg cgtttcatca
     2341 tgccgcgctg gagggccttg gcgagtttaa aggtcgcggt catattgacg tcaatgacgg
     2401 tttgccagtc ttcgtcgctc atgcgcatcg acagaccgtc acgggtcagg ccggcgttat
     2461 tgaccagaat atcgacctgg ccaagcgctt catcggcagc ctttaccaaa tcggcgatgg
     2521 cgttttcctg agagagatcc gcaggcagga caaaaacacg cttgcccagt tcggtggcca
     2581 gcgtattaag cttttccgtg ttgcgcccgg agatgccgac ggttgcgccc gcgctatgca
     2641 gagccttggc aatggccccg ccaatcccac ctgtggcacc ggttacgagg gctgtttttc
     2701 ctgttagatc gaacatgttt tctccttaaa atcgtcattg cgagcgtagc gaagcaattc
     2761 atctgcgaca agcttaaaaa ctggattgcc gcgtcgggct gcaccctcct cgcaatgacg
     2821 cagttacttc aatgaattga taatcgtttc aatttcttcc ggcgtgcctg tattggcgca
     2881 ggatatatca cgatcgatac gccgcacaag cccgcaaagc actttgcccg cacccagttc
     2941 gatcatttca tcaacgccct gatctttcat ccagaggaca gattcgcgcc aacggactgt
     3001 gccggtgacc tgctcgacca gaagcccgcg aatttcatcc gggtcgctgg tggctgcggc
     3061 tgtcacgttg gcaacaaccg gcacgatcgg ggctttgata tcggtcgcgg cgagggcttc
     3121 ggccatggca tcggcggccg gttgcatcag cgcgcagtga aacggggcgg atacaggcag
     3181 ggtcacagcc tttttcgcgc cgcgcgcggt ggccagttcg acggcgcggg ccacggcgtc
     3241 cttgtggccg ctgaccacga cttggccgac ggagttatca ttggcagcag cgcaaatgtc
     3301 actgcctgca gcttcttgcg caatggcctg cacatcctcg aaagacgggc cgataatcgc
     3361 ggccatggag ccaataccca ccggcacggc cttttgcatc gcttggccgc gaagctttag
     3421 cagtctggcg gcatctgcga tggtgaaact ttcagcggcg gtcagggccg catattcgcc
     3481 caaagagtgg ccggcgacga accgggcggc ggttttaaaa tcaatgccgc cctgtttttt
     3541 taagacctgc accacggcca tggaaacggc catcaaggcg ggctgcgtat tttcggtgag
     3601 attaaggtct tcttcgggcc cttcaaacat caatttcgaa agattttgcc cgagtgattc
     3661 gtcaatttct tcgaaaactt cgcgcgcttc aggaaaagtt gcatacaaat ctgctcccat
     3721 gcctacgctt tgtgatcctt gtcccgggaa tataaaagcc cgcgtcattt ttagctccaa
     3781 tcttttgttt tttcaatcat attagcgaaa tataatttcc tatttataat ggtaaataag
     3841 aattttatat cacctatcgt tacaaaacct ttgcaaaaag tttgcatttt ttttgtaaat
     3901 atgcgagttt ttatctgctt cgcataatct ggtgttttta gttcctatag cgctgagaga
     3961 aaattttcaa ctgcggagta tcaataagaa gtctcacctg aatgagacac cccataaaaa
     4021 gtaggccggt tttgaggatg aacggaggag acgacatgac acagaatatt cacacacgca
     4081 tcaagcattt gcttgaagag actaacgatg cttttgcaag cagcggtacg attaatatgg
     4141 attatgccgg atttgcggcg atggcgctgt ctgattttaa aaacttgctg ggtaatccgg
     4201 atcttacgga tatggaactg cgccgcgtga tccgtagcgg tgagaaaaaa cgccgcttga
     4261 aggatcctaa cggctgctgg gcaagcttta ttgctcatta cgttgctcgc aatgccaacc
     4321 aaaatcttaa agaacagtgt acgctataga tcttggcgaa atttataaag aaagtccttg
     4381 attttaactg ctggctgggc ttatagtccg cctcaattca taacctcgct ggtttaagaa
     4441 ggtttaagcc agctttttta gtgcgttcca tgccgaattg ttcggtcttt ttgggggggc
     4501 acgtaaagta accgagagga gggcaatatg ccaacttacg aaaccgtgtt tatgacacgg
     4561 caagatctta ccgaaggtca ggtcaaagac ctcaccgaca aatacgccaa agtcatcacc
     4621 gaccaaggcg gtaaaatcct gaaaactgag caatggggcc tgcgcacgct ggcttacaaa
     4681 atcaataaag cccgcaaggc gaattacacg ctggtggaat ttgaagcgcc aggcgcggct
     4741 gttattgagc ttgagcgcct gatgcgtatt gacgaagacg ttatgcgctg cctgtccttg
     4801 cgtttggatg ctccaaccga aggcccgtct gtcatgatgg aaaaaaaccg cgatcgcgat
     4861 gatgacacaa ctatgaagaa ggaggctgca taatgtccgc agcagatgta ccgactgtaa
     4921 aacgtccatt tttccgtcgt aaaaaaacgt gcccttttac aggcccatac gccccggcga
     4981 ttgactggaa agatacaaaa acactgagcc gttatatttc cgagcgcggg aagattatcc
     5041 caagccgcat tacggccgtg tcgcaaaaga aacagcgtga actggccaaa gccatcaagc
     5101 gcgcccgctt catgggtctt ttgccttatg tacaaactga gaccgagcaa cgccaagcgc
     5161 gcccgcctcg ctcttaaggt ttaaggagat ataagatatg tctacacagg ttattttgtt
     5221 agagcgcgtg gaaaagctgg gcaatatggg cgatgttgtg tccgtgaagc caggctatgc
     5281 acgtaactac ttgttgccgc agaaaaaagc tctgcgcgcc agcaaggaca atgttgctta
     5341 ctttgaagca cagaaaaaac atctgcaggc ggaaagcgac aagcagaaaa aagaagctga
     5401 aaaacgcgcc aagaagcttg ccggcacaaa agttgctctc attcgtcagg cttctgaaag
     5461 tggccaactt tatggttcag ttgcctctcg cgatattgcg gcagccctta ccgaagccac
     5521 gggcgagagt attgatcgct ctatggtcaa gcttaatcaa aactataaac tgatcggcct
     5581 gttcccgatt gacgtaacac ttcaccctga agtgacggcc gaaatcacga tcaacattgc
     5641 ccgctcaccg gaagaggctg aaatccaggc caagactggc aaagctctgg ttgctgaagg
     5701 tcatggcgat gagccatctg aggatgagcg cgctgcagcg gcggatgaag ttttggaaga
     5761 tgctttggaa gaaggcgctt tggaagctga aaaagagcgt caggcagaag ccgaagaaaa
     5821 agctgcggaa gatgctgcga aggctgagga gcgtgctacg aaaaaggccg agaaagatgc
     5881 tgcgaaagcc gctgctgccg aagctgaaga agcagcagct gaggctgaat ctggtgaaga
     5941 caccccggca gaagaagaga gcgaagctta agcttcttca cttggaattt taaaaaggtc
     6001 cgccacgcgc gggccttttt gtttggattt tatttcaatg taaaacacac aaagttcgca
     6061 gggttttgag agtatatgtg acttcaaaat ccgtcccaat accgtccagc gctggaactc
     6121 cgcatttaat gtgcggtccc ttgcagttcg gggcccttca catggccccc gtttttaaaa
     6181 cggggtttct tcaaactgta tgacttggcg aagtttgggt tgaatgcaat ccatccccgt
     6241 tattcacaga catgtttaca gcttttctgg tatggatgcg ggtgctcggt tatggtgggg
     6301 acatggtaca cgatcttgca tttcaggcgc tcgaaggcgt ctctcacgtt tctggagacg
     6361 ccacgccgaa ttaccgcatt ttgccgcata accttgatgc cgagcagggt ttgctgggcg
     6421 cgcttttggt ggataaccgc gccgtggaga aaatgggcga cttccttaag cctgcgcatt
     6481 tctttatgcc cgcgcatcaa cggatttttg aaacgatttt gacgctcatt gagcggg
//
LOCUS       DAOSWR010000114         6535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7722_length_6535_cov_5.092438, whole genome shotgun sequence.
ACCESSION   DAOSWR010000114 DAOSWR010000000
VERSION     DAOSWR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_6535_cov_5.092438"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..501
                     /locus_tag="PK513_08305"
     CDS             223..501
                     /locus_tag="PK513_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO82489.1"
                     /translation="MQSVLSSRIPCKSANGKIGEYEKSKALLQEAIERFDSNVYRVFL
                     AMTLFNLNEHAEALSLLLNVIVQTSQDEGIKRYGKAIDYYSDKLDMKW"
     gene            495..647
                     /locus_tag="PK513_08310"
     CDS             495..647
                     /locus_tag="PK513_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82490.1"
                     /translation="MVSPEGLGLFRSVPEQVKRLLDELRGADCFLVILTCAVMGCRRG
                     EALGLY"
     gene            complement(1168..2538)
                     /locus_tag="PK513_08315"
     CDS             complement(1168..2538)
                     /locus_tag="PK513_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO82491.1"
                     /translation="MKSFGKYITRHLACFAATIIVLFLVNAVSFGWTFHEVITRDYGD
                     ASPQNMLEDVAAASTQDGISDDARRKLQNNSIWAMYLDREGQCAWSVDKPVNLPDIYS
                     IQDVAVFSKGYLADYPVFIWNAGDGLLVLGYPKDSYTKLTSNYFSIHAIQTLPLYFSG
                     MLAVDLLLLFLAYYFSKRKIIRNTEPIVASIEALSSGKPVSLSGCGELSDVAESVKKA
                     SRIISRQNEARANWISGVSHDIRTPLSMIMGYAGRIAESSAAGSSVKEQANIIRNQSI
                     KIKELVQDMNLVSQLEYEMQPLHKAPARLSKLLRSYVADLINAGVPEIYSIGIEITPI
                     AETTVMNCDSRLISRAVSNLVQNSMKHNPQGCHIGLSLDRFDTSLILVIADDGIGISA
                     EKLQELDRKPHYMEGTDETLDVRHGLGLLIVRQVTEAHGGTMQISNATDHGFTTILNF
                     PIGIVE"
     gene            complement(2543..3244)
                     /locus_tag="PK513_08320"
     CDS             complement(2543..3244)
                     /locus_tag="PK513_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO82492.1"
                     /translation="MNRSFLHGKKLLLVDDEQDLLQMATAILADEGFENIITATSVRE
                     GIDAADQEKPDLAILDVMLPDGDGFSLMGRIRAITDIPIIFLTAKDEAADKLAGLRLG
                     ADDYIVKPFLPQELLLRVYAILRRCYKEDAPLVKLEGSTVDFSRAEVIKANEVIPLTA
                     KEHILLETLARSEGRIVTVDTLCEALWGDNPFGYENSLNAHVRRIREKIETDPSKPVS
                     LITVKGLGYKLHTRK"
     gene            3386..4150
                     /locus_tag="PK513_08325"
     CDS             3386..4150
                     /locus_tag="PK513_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016805738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO82493.1"
                     /translation="MDYSITTKHLTKNYKNFTSVSNVSLHIRKGSIYGLLGPNGAGKS
                     TTMKMLLGLTEPSKGSFTIDGKHFPADRLAILSEIGSFIEAPSFYANLTGRENLDIIR
                     RILRLPQSSVGDALDLVGLTEFGDRLAKKYSLGMKQRLGLAGALLGKPPILILDEPTN
                     GLDPSGIHEIRNLIKTLPALYDCTILISSHMLSEIELIADDIGILNHGRLLFEGSLSD
                     LRQGAQQSGFAADNLEDVFLSMVEKDNASRKRRAQL"
     gene            4147..4872
                     /locus_tag="PK513_08330"
     CDS             4147..4872
                     /locus_tag="PK513_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009994320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO82494.1"
                     /translation="MSTLAIELRKEKRTGVIPVLLAVGVLGAAYAFVNFFGRKDTLLN
                     LPLAPMDVLLTQLYGMIMILNMFGIVVAACMIYNMEFKGNAVKKIYMLPTSVPGMYFC
                     KFLILTVLLFIAIAIQNLALARIGMSDLPQGTFEMNTLTAFAAYSFITSMPVLSFMLL
                     ISSRFENMWVPLGVGVAGFLSGMALATSKSALLMIHPFVVMLKPAVAMVAQPDCTVTT
                     VSVAETIIFLVAGLWMAKHLRYE"
     gene            4888..5652
                     /locus_tag="PK513_08335"
     CDS             4888..5652
                     /locus_tag="PK513_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO82495.1"
                     /translation="MRFTELLKIEFMKVKRSKIVPLIFVAPLLVVASGIVNLSSYFTP
                     EYTNAWPAMFIQSALVYAYYLLPLSMIVVCVMIAGRETQNNGVLKMLTLPVGRYALSL
                     AKFCVLVVYLFMEMAVFFAVFIFAGLIATNTMGVKETLPILYLTKWCVWLFLTMLPSV
                     ATIWAITVLFEKPLFSVGLNLLLVIPGVLVANTPLWIAYPYCYSGYLVSCSLHEFTTA
                     SADTAFSMMPFLPCAILAFLLALGLAITQFGKKEMR"
     gene            5658..5954
                     /locus_tag="PK513_08340"
     CDS             5658..5954
                     /locus_tag="PK513_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82496.1"
                     /translation="MKNKTLQKLSVAALVASVLPLAAFLPALLNLTLSEGVRNIWAGA
                     NIAFILMGLSLSIVCMQSQDKKSIVNIVSTIISAFLLLIIIGIGVLALFVNFLQ"
     gene            complement(6184..6309)
                     /locus_tag="PK513_08345"
     CDS             complement(6184..6309)
                     /locus_tag="PK513_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82497.1"
                     /translation="MREEVLPRNQLANRPVRAVFAFFVICALVRKWLKTYWLMPG"
BASE COUNT         1624 a         1598 c         1528 g         1785 t
ORIGIN      
        1 aatatgcatg ctgaggtggt agaaatgcgc ttttgggatt ttagaaacac accctagttt
       61 atcacacatc cgtttagttg agtataaaca ttatgtgtat tcgctcattc aaaaaagctt
      121 cgggcaattg aggaaacacg acggcctttt cgccgtaaac agtccacatg gaaggcggta
      181 aaccgcgtga tgtgtccttt tgtaaaacaa gtgtattatt aagtgcaatc tgttctttct
      241 tcacgtattc cctgtaaatc ggctaacgga aagattggcg agtacgaaaa atcgaaagcc
      301 ctgcttcagg aagcgattga acggtttgat tccaatgtat atcgggtttt tttagcgatg
      361 accttgttta atctgaacga gcatgccgaa gcgctgtctc tcctgttgaa cgtgattgtt
      421 caaacgtctc aggatgaagg aatcaaacgg tacggcaaag cgattgatta ctactccgat
      481 aagctggaca tgaaatggtg agcccggaag gtttggggtt gtttcgctcc gtgccggagc
      541 aagtgaagcg gttgcttgac gaactgcgcg gcgcggactg ttttcttgtg atccttacat
      601 gcgctgtcat gggatgcagg cgcggcgagg ctttgggcct ctactgatcg ggtattgact
      661 ttgaaacatc cacgctggaa ttcaagcgcg cgctgatcgt cagcagcttg acaaacacag
      721 tagagataat ctccaagttc agtctcgctg ttcgaaaata caactttgaa tatctcaaga
      781 taactatccc gcaaatattc cttcactgat ttgccgccat ttgcgctgct ggttaaaaaa
      841 tttgcaatac ttttttttca tatagaatgc ctccaaagaa ttccaatcat aaaaaaccaa
      901 ttgatggcct cagcgcccga tttgggggca aattcggccg gcggcatgta tggcgctgca
      961 gtcctttcgc cggcgctcca ttttgtccct cgtatcctcc ggagacatga gccagtttat
     1021 attcaggcat tcgtcccggc agccgctaac gcccgctcga aagcaataat gataaaatcc
     1081 aatgtcgata aatactcggc aacagtactg cgaacgaatt aatttgcaat aattaggttc
     1141 gaatgtttaa tccttttaat tttggaacta ttccacgatt ccaatcggaa agtttaatat
     1201 tgttgtaaat ccatgatctg tagcgttcga tatctgcatg gtgcccccgt gagcctccgt
     1261 aacctgtcga acaattaaaa gtcccaaacc atgtcttacg tcgagcgttt cgtccgtgcc
     1321 ttccatgtaa tgaggttttc gatccagttc ctgcagtttc tcagcggata tcccaatgcc
     1381 gtcatcggca atcacaagaa tgagagaagt atcgaaacgg tcaagggata gcccgatatg
     1441 acacccttgc ggattgtgtt tcatgctatt ttgcaccaaa ttgcttacag cacgtgatat
     1501 taatcgagag tcgcagttca ttacagttgt ttcggcaatc ggcgtaattt caatcccaat
     1561 ggaataaatc tccggaacgc ctgcattgat taagtctgcc acataagaac gcaatagctt
     1621 tgataatctg gcaggcgcct tatgcagcgg ctgcatttca tattccagct gcgataccaa
     1681 attcatatcc tgcaccagtt cttttatttt aatactctgg tttcggataa tattcgcctg
     1741 ctctttaaca gagctgcctg cggcactgct ttccgcgatt cgtcctgcat agcccataat
     1801 cattgacaga ggcgttcgta tatcatgcga tacgccgctg atccaattag cacgcgcttc
     1861 gttctgcctg ctgattattc gtgacgcttt cttcacgctt tcagcgacat cggacagctc
     1921 tccgcacccg gaaagcgaaa caggctttcc gcttgagagc gcctcgatgg aggcgacgat
     1981 cggttccgtg tttcgaatga tttttcgttt agaaaagtaa tatgccagaa aaagaagcag
     2041 caagtcaacc gctagcattc ccgaaaagta tagtggtaat gtctggatgg cgtgaatgga
     2101 aaaatagttg cttgtcagtt ttgtatagct gtctttcgga taacccaata ccagcagtcc
     2161 gtccccggca ttccatataa aaacagggta atcggccaga taaccttttg aaaaaacggc
     2221 cacatcctga atggaataga tgtcgggcag attcaccggt ttatcgaccg accacgcgca
     2281 ttgtccctcc cggtctaaat acatagccca aatggaattg ttctggagct ttcggcgggc
     2341 atcgtccgaa ataccatctt gcgtagaagc tgcagcaaca tcttccagca tattttgagg
     2401 agaagcgtca ccataatccc gcgttataac ttcatgaaat gtccagccga aagaaactgc
     2461 attaaccaga aacaacacaa ttatagtagc ggcaaaacag gccaggtgtc ttgttatata
     2521 tttcccaaaa gatttcatag cttcacttcc tcgtatgcag cttataaccc aatcccttaa
     2581 ccgtgatcaa agaaaccggc tttgacggat cggtttcaat tttctcccgt atacggcgga
     2641 catgggcgtt caagctgttt tcatacccaa acgggttatc tccccaaaga gcctcgcata
     2701 acgtatcaac cgttacaatt cttccctcgc ttcgggcaag tgtttcaagc agaatatgtt
     2761 ctttcgccgt cagcgggatc acttcattcg ccttaattac ttctgcgcgg ctgaagtcga
     2821 ccgtcgatcc ctccagcttc accaacgggg cgtcctcttt gtagcaccgc ctcagtatcg
     2881 cataaacgcg cagcagtaat tcctgtggca agaacggttt cacaatataa tcgtccgcgc
     2941 caagtctcag ccctgccagc ttgtccgccg cttcatcttt tgccgtcaga aaaatgatcg
     3001 ggatatccgt aatggcacgt atccgtccca taagggaaaa cccgtccccg tccggcagca
     3061 tcacatccaa aattgccaaa tccggcttct cctgatcggc ggcgtcaatt ccctctcgga
     3121 cggaagttgc cgtaataata ttctcaaacc cttcatccgc caggatggcc gttgccattt
     3181 gaagcaaatc ctgttcgtcg tccaccagca gaagcttttt tccgtgtaaa aaagagcgat
     3241 tcattacatc acctccaaca aaaattataa catggcgcat cgatattcag actgcctcca
     3301 cgaaaaagta aggtaaaagt aagcgtacgg aaatgcaggc gtcatggcga cgctgtagta
     3361 tatggcgtcg aaaggagtgg tttatttgga ctatagcatt acaacaaagc acttaacaaa
     3421 gaactacaaa aattttactt ccgtcagcaa cgtttcgctt catatccgca aaggcagtat
     3481 ttatggcttg ctgggaccta acggagctgg aaagtcaact accatgaaaa tgcttttagg
     3541 attgaccgaa ccctcaaagg gcagcttcac catcgacgga aaacactttc ccgctgaccg
     3601 gcttgcaatt ctgtcagaga tcggctcctt cattgaggcg ccctctttct atgcgaacct
     3661 gacaggccgc gagaaccttg acatcatccg caggattctg cggcttccgc aaagttcggt
     3721 aggagacgcg ctggatctgg ttggccttac cgagtttggt gacaggcttg caaaaaaata
     3781 ctcgctggga atgaagcaac gcttggggct tgccggagcc ctcttgggaa agccacccat
     3841 tcttatatta gatgagccaa caaacggtct tgacccttca ggcatccatg aaatccgcaa
     3901 tttaataaag acccttcccg ctctttacga ctgcacgata ttgatttcct cccacatgct
     3961 gtcggaaatc gagctgatcg cagacgatat tggcattctt aatcatggcc gactgctgtt
     4021 tgagggaagc ctgagcgatc ttcgtcaggg cgcgcagcaa tccggtttcg ccgctgacaa
     4081 tctggaagat gtgttcctct ctatggttga aaaagataac gcgtcccgaa aacggagggc
     4141 gcagctatga gcacgctcgc aatcgaactc cggaaagaga aacgaaccgg cgtaatccct
     4201 gtcctgctgg cggttggcgt cttgggggcg gcgtacgctt ttgtcaattt ctttgggcgg
     4261 aaagatacgc ttctgaacct gccgctggct ccgatggatg ttctgctaac ccagctctac
     4321 ggcatgatca tgattttaaa tatgttcggc atcgtagtag ccgcctgcat gatttacaac
     4381 atggagttta agggtaacgc agttaaaaaa atctatatgc tgccgacaag cgtcccggga
     4441 atgtacttct gcaaattcct gatattgacc gttctcctgt tcattgcgat tgcaattcaa
     4501 aacctggcgc tcgcgcggat cggcatgtcc gatttgccgc agggaacctt tgaaatgaac
     4561 acgttgacag cctttgcggc atattccttt ataacttcca tgccggtgct gtcctttatg
     4621 ctgctgattt cgtccaggtt tgagaatatg tgggttcctc ttggggtagg cgttgccggt
     4681 tttttaagcg gaatggcctt ggccacgtcc aagtcggctc tgttgatgat tcatccattt
     4741 gtcgtgatgc tgaaacccgc cgttgccatg gtcgcccagc cggactgtac cgtgaccacc
     4801 gtttccgtgg ccgaaacaat catttttctg gttgccggac tatggatggc aaaacacctg
     4861 cgttacgaat gaggagggag gtttgaaatg cgttttacag aactgcttaa aattgaattc
     4921 atgaaagtga agcgttcaaa aattgtccct ttgattttcg ttgccccatt attggtggta
     4981 gcgtccggaa ttgtgaactt gagcagctat ttcaccccgg aatatacgaa cgcgtggccg
     5041 gccatgttca ttcaaagcgc actggtctat gcctattatt tgcttccgct gagcatgatc
     5101 gttgtgtgtg tcatgattgc cggacgggaa acgcaaaaca acggcgtctt aaaaatgctg
     5161 accctgcctg tcggccggta cgcactttca ctggcaaaat tctgtgttct ggtcgtttac
     5221 ctttttatgg aaatggcggt tttctttgca gtatttattt ttgccgggtt gattgcaaca
     5281 aatacaatgg gagtaaagga aacgctgccg atcctgtatt taacgaaatg gtgcgtttgg
     5341 cttttcctca ccatgcttcc tagcgtagcg acaatttggg caataacagt actgtttgaa
     5401 aagccattgt tttctgtggg actgaacctg ctgttggtga ttccaggtgt cctggttgcc
     5461 aacacaccgc tctggatcgc ctatccatac tgttacagcg gatatttggt atcatgctcc
     5521 ctgcatgagt ttacaacggc atctgccgat accgctttct cgatgatgcc gttccttcct
     5581 tgtgccattc tagcttttct gctggcttta gggctggcga tcactcaatt tggaaaaaaa
     5641 gaaatgaggt aattattatg aaaaacaaaa ctctgcaaaa actgagcgtt gcggcacttg
     5701 ttgctagtgt tctgccgctg gcggctttcc tgccggccct gctaaacctc actttatcgg
     5761 agggggtacg caatatttgg gccggcgcca atattgcttt tatcctgatg ggcctttccc
     5821 tttccatcgt atgcatgcaa agccaggata aaaagagtat tgtgaatatc gtttcaacaa
     5881 taatcagcgc cttcctgctt ttgattatca ttggaatcgg cgtgctggct ttatttgtga
     5941 atttcctgca ataagataat cctcgcgaag ctgcggcgca gtgcggttct cgctttggct
     6001 gacaggatcg tgatcttctc cggcagtgag tgggatattt ggttttgcgt acaattcgtt
     6061 gccggggaag ggcttaaacc tgcgacctcc gggttatgag tgcatattaa tgcggttata
     6121 taatcatatt gatgcatttg gggacgcata tggccgcaag aggttgcggc agattgcctt
     6181 acatcaacca ggcatcagcc aataggtttt gagccatttc cgtaccaaag cgcaaattac
     6241 aaaaaacgca aaaaccgccc gaaccgggcg gtttgccagt tggttgcggg gaaggacttc
     6301 ttcgcgcatt tcagacacgt ttgctagcat tccatgcttc gcaaacggtc tcgttcgcaa
     6361 agccggccgc taaaaccgcc ggcactgcgg cgtttttgtc accgcgaaac gacggccgcc
     6421 gcggcttcaa gtccttttga aacacttgcc aaaaagcaga agccatccaa aatggatggc
     6481 ttctgctttt tggttgcggg ggaaggactt gaacctacga cctccgggtt atgag
//
LOCUS       DAOSWR010000115         6454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7859_length_6454_cov_5.548367, whole genome shotgun sequence.
ACCESSION   DAOSWR010000115 DAOSWR010000000
VERSION     DAOSWR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7859_length_6454_cov_5.548367"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK513_08350"
     CDS             complement(<1..433)
                     /locus_tag="PK513_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOO82498.1"
                     /translation="MNVYYDKDCDLELIKAKKVAIIGYGSQGHAHAQNIKDSGVKTVI
                     IGLREGSASTAKAQGAGFTVIPPAKAAAEADVVMILAPDEHQADLYRDDIEPNLKQGA
                     ALMFAHGLNIHFGLIKPRADLDVIMVAPKGPGHTVRGEYQKG"
     gene            complement(561..983)
                     /locus_tag="PK513_08355"
     CDS             complement(561..983)
                     /locus_tag="PK513_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82499.1"
                     /translation="MKNLPLALLAGSTLLLTGCIDRETADTKLVKGCKAGIEVFLYEG
                     DRIEKIVSQTLRQPTGLGSGYREVTLNATITDGFHPHDERYTCMFMEEFGPFKMSYRA
                     SIYNIDLDGRIIGQKGYEIQGDLKEMQKLTGAVDAALK"
     gene            complement(988..1563)
                     /gene="ilvN"
                     /locus_tag="PK513_08360"
     CDS             complement(988..1563)
                     /gene="ilvN"
                     /locus_tag="PK513_08360"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008885116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOO82500.1"
                     /translation="MQAIKKKNRNSKSVYAPNPLQQEEESHTLAIVVENEPGVLARVI
                     GRFTGRGYNIDSLTVAETDHEENTSRITIVCSGTPAVIEQIKAQLRQIVPVKRVRDLT
                     TFGPHIERELALIKVAGTGEKRIEALRLADVFRAQPLDSTLESFVFELTGTTEKVNAF
                     IDLMKPLGLVDVSRTGIAAISRGSTTHDDKD"
     gene            complement(1592..3388)
                     /locus_tag="PK513_08365"
     CDS             complement(1592..3388)
                     /locus_tag="PK513_08365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HOO82501.1"
                     /translation="MSTTEAKNHAPKATPNNPDQMTGAEIIIQALIDQGVEVVFGYPG
                     GAVLPIYDEIFKNQDKIKHVLVRQEGGALHAAEGYARSTGKVGCALVTSGPGATNAVT
                     GLTDALLDSIPLVCITGQVPTFLVGTDAFQEADTTGITRPCTKHNYLIKDANKVADII
                     HEAFHVARSGRPGPVVIDIPKDVQFAKGAYKKALKPSLQTYQPKTEADRAAIEAAVAL
                     MANAKKPVFYTGGGVINAGTEASKLLRELVRETGFPITSTLMGLGAYPASDKQWLGML
                     GMHGTFEANLAMHDCDVMINIGARFDDRVTGRTDKFSPHSTKIHIDIDPSSINKNIHV
                     DIPIVADAASALKMMIDVWKKHRYKADREALDKWRKQIEGWRAKNCLAYKNSDTVIKP
                     QYALERLRDALAKDPREKYITTEVGQHQMWAAQFLPFNEPNRWMTSGGLGTMGYGLPA
                     AVGVQMAHKDSLVVNISGDGSVQMNIQEIGTAIQYKLPVKIFILNNSWLGMVRQWQEL
                     LHGERYSETYSESLPDFVKLAEAYGGVGLRAETPDELDDVIEKMLKTDRLTIVDVCVD
                     QRENIFPMIPSGKAHNEMILSPDADKFDKSLV"
     gene            complement(4040..5413)
                     /gene="hemN"
                     /locus_tag="PK513_08370"
     CDS             complement(4040..5413)
                     /gene="hemN"
                     /locus_tag="PK513_08370"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOO82502.1"
                     /translation="MRCNLRETMLKLDRPVPRYTSYPTAPHFQPLEDEQLYPQMLSRL
                     PERSRLSLYLHVPFCPKMCWYCGCHTKITQRYGPVEDYAHLMIREIEILADALGDKGQ
                     RISHIHFGGGSPGMLRARDFDLVMSRIRKSFTIEETAEIAIEIDPRELTEGRVQAYAD
                     NGVNRISLGVQDFNDNVLEAVNRPQPFELSEKAVALFRGRGVDRVNMDLLYGLPHQTP
                     ETMKATIEKVIALDPDRVALFGYAHVPWMKKHMRLIDEGALPDKTMRFDLFESGALAL
                     EQAGYFPVGIDHFAKGNDPLVQAAKEGRLRRNFQGYTDDAADALIGVGASSIGFLPGG
                     YVQNAVDMPLYREQILASKLTAAKYCPISAEDALRADVIERLMCDFGVDVGGLCARHG
                     FEQEHLDVEIAALEPYKGLDFVQMEGRVLTMNPKARMMVRIVASVFDAYIPNADTPVP
                     QRHSKAI"
     gene            complement(5444..5878)
                     /locus_tag="PK513_08375"
     CDS             complement(5444..5878)
                     /locus_tag="PK513_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO82503.1"
                     /translation="MHKTLVSELMTETVVRVGADDNLTTAAQKMKEADCGVLPVGNGD
                     NLQGMITDRDIVIRALAEGKNPDEAKVGDYMTNKVYACNEEDFLEDAADKMRSHNVSR
                     LIVRNHEGQVTGILSFGNILRKNADAEEVAKIVERAVFKVVA"
     gene            6062..>6454
                     /locus_tag="PK513_08380"
     CDS             6062..>6454
                     /locus_tag="PK513_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82504.1"
                     /translation="MLKHLCGVLVAGVAFVAAPLEGLATDSERAMLETMFKEADIIPD
                     GKLDEGEFDMYHFSAFVGMDSNHDGVLDKRECNDDCFGYNAGNGVTNQQSRPYQKLEF
                     AQTPYRFDAIDKRWSRFFGQLCGEVKLRS"
BASE COUNT         1447 a         1579 c         1669 g         1759 t
ORIGIN      
        1 cgcctttttg gtattcgccg cgcacagtat ggccgggccc tttgggggcg accatgatga
       61 cgtcgaggtc ggcgcgcggt ttgatcaggc cgaaatggat gttcaggccg tgggcaaaca
      121 tcagggctgc gccttgtttg aggttgggtt cgatgtcgtc gcggtacagg tcggcctggt
      181 gctcgtccgg ggcgaggatc atgacgacat cagcttcggc tgcggctttg gccgggggta
      241 tgacggtgaa gcctgcacct tgtgctttgg cggtggatgc ggagccttca cgcagaccga
      301 tgatcacggt tttaacgccg ctgtctttaa tgttttgcgc gtgggcatgg ccttgtgagc
      361 catagccgat gatggcgact ttcttggctt tgatcagttc gagatcacag tctttatcat
      421 agtaaacatt caggccggtc gtgttttcag gcgtcgtttg cgccgctgct tgtgtcataa
      481 tcgtgaacct tttttaaatt ctatatttgt gaaccgcata aaacagcctt tggacggcta
      541 aatcaagcta ttatttgttg ttatttcagc gctgcatcga ctgcgccggt tagtttttgc
      601 atctctttaa ggtcgccctg aatttcatag cctttttggc cgatgatgcg accgtccaga
      661 tcaatgttat aaatgctggc acggtacgac attttaaagg ggccgaattc ttccataaac
      721 atgcaagtat agcgttcatc atgcgggtga aagccatcgg ttatggttgc attcaacgtg
      781 acttcgcgat agccgctgcc cagacctgtg ggttggcgca gagtttgcga gacgattttt
      841 tcaattctat cgccctcgta aagaaacact tcgataccgg ctttgcagcc tttgaccagc
      901 tttgtatctg ctgtttcacg gtcgatgcag cctgttaata gcagggttga tccagcaagc
      961 agtgcgaggg gcaaattttt catggcgtta atccttatca tcatgggtgg tagagccgcg
     1021 ggaaatggcg gcaatgcccg tgcggctgac atcgacgagg cctaaaggct tcattaagtc
     1081 aatgaaggcg ttgacttttt ctgttgtgcc agtgagttca aagacgaaag attccagcgt
     1141 ggaatcgagc ggttgagcgc ggaaaacatc ggcaagcctt aaggcttcga tgcgtttttc
     1201 tccggtgccg gcaactttga tgagcgctag ttcgcgctct atatgcgggc cgaatgtggt
     1261 gaggtcgcgc acacgcttga cgggtacgat ctgccggagc tgggccttga tttgttcgat
     1321 aaccgctggc gtgcctgagc agacgatggt gatacgcgag gtgttttcct cgtgatcggt
     1381 ttcggcgacg gtcaagctgt cgatattata gccgcggccg gtaaagcggc caatgacgcg
     1441 ggctaaaacg cccggttcgt tttctacgac aatcgccagc gtatggcttt cttcttcctg
     1501 ctgcaacgga tttggcgcat aaacggattt tgaattgcgg tttttctttt tgattgcctg
     1561 gatcataatc ttgatgtcct tgttttagtc tttataccaa actcttatcg aacttgtcag
     1621 catcggggga caggatcatt tcattgtgag ccttgccgga agggatcatc gggaagatgt
     1681 tttctctttg gtcaacgcac acatcaacga ttgtcaggcg atcggttttg agcatttttt
     1741 caatgacatc atcaagttca tccggtgttt cagcgcgcag accaacacca ccataggctt
     1801 cggcaagctt gacgaagtcg ggcaggctct cggagtatgt ctcagaatag cgctcgccgt
     1861 gcaagagttc ctgccactgg cggaccatgc ccagccagct attatttaga ataaagatct
     1921 ttaccggtag tttgtattgg atggcggtgc cgatttcctg aatgttcatt tggacggaac
     1981 cgtcgccgga gatattgaca accagagaat ctttgtgtgc catttgtaca ccgacggcgg
     2041 cgggcaggcc gtagcccatg gtgccaaggc cgccggaggt catccagcgg ttgggttcat
     2101 tgaacggcag gaattgcgcg gcccacatct ggtgctggcc aacctcggtg gtgatgtatt
     2161 tttcgcgggg gtctttggca agcgcatcgc gcaggcgttc gagcgcgtat tgcggtttga
     2221 ttaccgtgtc gctgttttta taagcgaggc agttttttgc gcgccagcct tcgatctgtt
     2281 tcctccattt gtccagcgct tcacgatcgg ctttataacg gtgttttttc cagacatcga
     2341 tcatcatctt gagcgcgctg gcggcgtcgg caacgatggg aatgtcgaca tggatatttt
     2401 tattgattga gctggggtcg atatcgatgt ggattttggt ggaatggggt gagaatttat
     2461 cggtgcgtcc ggtgacgcgg tcgtcaaagc gtgcgccgat attgatcatg acgtcacaat
     2521 catgcatcgc gaggtttgct tcgaatgtgc cgtgcatgcc gagcatgccg agccattgtt
     2581 tgtcgctggc cgggtatgcg cctaggccca ttaatgtgga ggtgatcggg aagcccgttt
     2641 ctcggacgag ttcgcgcaaa agctttgagg cttcggtgcc ggcgttgata acgccgccgc
     2701 cggtgtaaaa aacaggtttt ttagcgttgg ccataagggc gacagcagct tcgattgctg
     2761 cgcggtcggc ttccgttttg ggttgatagg tttgcaggga tggttttagc gcttttttat
     2821 aagcgccttt tgcaaactga acgtctttag ggatatcaat aactacgggc ccggggcggc
     2881 cagagcgcgc gacgtggaag gcttcgtgta taatatcggc aaccttgttg gcgtccttta
     2941 ttaagtaatt atgtttggtg caggggcgcg tgatgccggt ggtgtctgct tcctgaaatg
     3001 catcggtgcc gaccaggaag gtcgggacct gtccggtgat gcaaacgagg ggaatggaat
     3061 cgagcagggc atcggtcagg ccggtgaccg cattggttgc gccggggccg gaggtgacga
     3121 gcgcgcagcc aactttgccg gttgagcggg cgtagccttc ggcagcgtgg agggcgccgc
     3181 cttcctggcg gaccaggacg tgcttgattt tgtcctgatt tttgaaaatt tcatcataga
     3241 tgggcagaac cgcaccgccc ggatagccaa agacaacttc aacaccctgg tcgatcaggg
     3301 cttgaatgat tatttccgca ccggtcatct gatcggggtt gttcggggtg gcttttgggg
     3361 cgtggttttt tgcttctgtt gtgctcatca ttttctcact ttaataaaat tgcatttgtt
     3421 ttaacgtctt ccacaggctt tacgcaatat aaaacaaaga tttgggccat aaaaaaacgc
     3481 cctttgatca atgtcttcgg gcgcttggtc ggtttgaaaa aattgacaga tcctagtgcg
     3541 cccgtgcctc caccagaagt acgacgaggt cgctgacgac gttcacgagg atgttgttca
     3601 aagttttcat gtctcttttc taacgctttt catggtcttt tgtaaacact taaagcgttt
     3661 tttcggattt tagtgatcct ctgttttgga ggaaggatcg cttagccctt aaaaacgctt
     3721 tggttgtatg gcctgtttcg cggcctttta tacaaggttt gtccttggtc tttgttttgg
     3781 tttcgacaga ccgtttttta tacaccctta ttagaaaacc gcttttttac gtttgtccgt
     3841 gtctgtcttg atagattaac ttaagcatta aaattacttt ttgtcaatat aaaaagtaat
     3901 aaaaatacgt atttttactt aaaaagaaat aatattactt tattgagttc agttttggga
     3961 ctgatttgcg gtgcagtgca atcgggcaaa gggttttgag tctagccaga ggtgttggct
     4021 atgttgtaga aagagcgtac taaatcgctt tggaatgccg ctgaggcact ggcgtatccg
     4081 catttgggat ataggcatca aaaacagacg cgacaattcg taccatcatc cgcgccttgg
     4141 gattcattgt caaaacgcgc ccctccatct gaacaaaatc aagcccttta tatggttcaa
     4201 gcgccgcaat ctctacatcc aaatgctctt gctcaaaacc atgccgggcg cacagccccc
     4261 ccacatcgac gccaaaatca cacatcaacc gctcaatcac atctgcgcgc aaagcatctt
     4321 ctgcactgat cggacaatat ttcgccgccg tgagcttgct cgccagaatc tgctcgcgat
     4381 aaagcggcat atccaccgca ttttgaacat acccacccgg caagaaacca atagaagatg
     4441 ccccgacccc aatcaacgca tcggcggcat catctgtgta tccttgaaaa ttccgccgca
     4501 agcggccttc ttttgccgcc tgtaccagcg gatcattccc ctttgcaaaa tgatcaattc
     4561 ctaccgggaa atagcctgcc tgctctaacg ccagcgcccc cgattcaaac aaatcgaaac
     4621 gcatcgtttt atcgggaagc gcgccctcat caatcaaacg catatgcttt ttcatccacg
     4681 gcacatgtgc ataaccaaac aacgccaccc ggtcaggatc aagcgcaatc actttttcaa
     4741 tcgttgcttt catcgtttcc ggcgtctgat gcggcaaacc ataaagcaaa tccatattga
     4801 cgcgatcaac cccgcgcccg cgaaacaacg caaccgcctt ctcactaagc tcaaaaggct
     4861 gtggccggtt cacagcctcc aaaacattgt cattaaaatc ctgaacgccc agactaatcc
     4921 ggtttacgcc attatcggca taggcctgaa cgcgtccttc ggtcagctcc cgcggatcaa
     4981 tctcaatggc aatctcggca gtttcttcaa ttgtgaaact cttgcggata cggctcatca
     5041 ccagatcaaa atcccgtgcc cgcagcatcc ccggcgaacc gccgccaaaa tgaatatgcg
     5101 agatgcgctg ccccttatcg cccagcgcat cggcgagaat ctcaatctcg cggatcatca
     5161 aatgtgcata atcctcaacc ggtccgtaac gctgcgtgat tttggtatga cacccgcaat
     5221 accaacacat tttcggacaa aacggcacat gaagatagag cgacaaccgc gacctttccg
     5281 gtaaacggct aagcatttgc ggataaagct gttcgtcctc aagcggctga aaatgcggtg
     5341 cggtcgggta gctcgtataa cgcggcactg gccggtcaag cttcaacatg gtttctcgca
     5401 agttacaacg catttttaag gccttaatgt cgcggttaag cgcttaagcc acaactttaa
     5461 aaactgcgcg ttccacaatc ttcgcaactt cctcggcatc ggcgtttttg cgcagaatat
     5521 tgccaaagga caaaataccg gtcacctggc cttcgtgatt acggacaatt aaacggctta
     5581 cattgtggga acgcatctta tcggcggcat cctcaaggaa atcctcttcg ttgcaggcat
     5641 aaaccttatt ggtcatgtaa tcgccgactt tggcttcatc cgggttcttg ccttccgcaa
     5701 gcgcgcgtat cacaatatca cgatctgtga tcatcccttg caaattatcg ccgtttccca
     5761 cgggcaatac accacaatcg gcctctttca tcttctgcgc tgctgtcgta aggttatcat
     5821 ctgcgccaac tctgacaacc gtttcggtca tcaattcact gactagtgtt ttgtgcataa
     5881 taaaactcca tctctaactt acccctcaca atgaagagta ttgttcgttc cgatcctaaa
     5941 gcaatatcaa aaaagcaaag gacgctgcaa gaaacaaagc ctgctttttt gacaaatatc
     6001 aagtgcttgc tttggcattt atgtttggct catggcgtag aaatagaaaa aggatcttgc
     6061 catgttgaag catctatgcg gtgttttggt tgccggggta gcgtttgttg ccgcgccttt
     6121 agagggctta gcaacggaca gcgagcgcgc tatgttggag acgatgttta aagaagcaga
     6181 cattattcct gacggcaaac ttgatgaggg agagtttgat atgtaccatt tttctgcctt
     6241 cgttggcatg gattccaatc atgatggtgt tttggacaaa cgggagtgta acgatgattg
     6301 ctttggctac aatgcgggga acggtgtgac caatcagcag tccaggccct atcaaaagct
     6361 ggagtttgca cagacacctt accgctttga tgccattgat aagaggtggt ctcggttctt
     6421 cggccagctt tgcggcgaag ttaagctacg atcg
//
LOCUS       DAOSWR010000116         6416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_7920_length_6416_cov_5.406068, whole genome shotgun sequence.
ACCESSION   DAOSWR010000116 DAOSWR010000000
VERSION     DAOSWR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_6416_cov_5.406068"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2105)
                     /gene="alaS"
                     /locus_tag="PK513_08385"
     CDS             complement(<1..2105)
                     /gene="alaS"
                     /locus_tag="PK513_08385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO82505.1"
                     /translation="MKTVNDIRSEFLSFFKGKGHTVVESSPLVPQNDPTLMFVNSGMV
                     QFKDVFTGKDKRPYTRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDDAIAFAWELVTKNFALPAEKLCVTVHSSDEEAAAIWKKVSGLADEKIIRIPTDDNF
                     WRMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNNVFMQYEQVDADTRI
                     DLPAQSVDTGMGLERIAATLMGSHDNYAIDIMRGLIEHSADLTGVDPDGDMSASHRVI
                     ADHIRCSAFLMADGVMPSNEGRGYVLRRIMRRGMRHAHLLGAQDPLMCKLFPTLLEKM
                     GEAYPELGRAESLIVETLKSEEERFKLTLDRGLKMLEEETEKVSSGKKFPGDVAFKLY
                     DTYGFPLDLTQDALKSQNIEVDEDGFNTCMEEQKAKARAAWSGSGDAATEKIWYDILD
                     DFGPTEFLGYEVEAAEGQILAIVKDGHRAEMLKSGEEGYIITNQTPFYAESGGQVGDK
                     GNLTHTNGDIEVIDTKKFVGSIFAHRAQTANDVKVGENVQMEVNHMRRSAIRANHSAT
                     HLMHEALRRVLGDHVAQKGSLQDAQRTRFDISHPKAIKPEEIARVEEIVAAEIKADTP
                     VTTRLMGLDEARETGAMALFGEKYDSEVRVVAMGTKDKNREFSIELCGGTHVKKTGEI
                     GPFKIISESALSAGVRRLEA"
     gene            2145..2897
                     /locus_tag="PK513_08390"
     CDS             2145..2897
                     /locus_tag="PK513_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOO82506.1"
                     /translation="MNEFVTLAKRLADEAGKIVKRYYRQPFDVISKEDESPVTIADRK
                     IEERMREILEDARPQDGIYGEEHGVKTGESGLTWVLDPIDGTKSFVIGRPTFGTLIAL
                     CEDSIPKLGVIDQPILGERWIGDGEQTFFNGRPVQCAPCASIETARIGSTTPAMFEAT
                     GPVYKNFNEGRFFWGGDCYQYGLMACGFVDIILEADMKPYDYAALVPIVQGAGGHISD
                     FSGAALILESDGSVVACGDPGLWPEMHKRLSA"
     gene            complement(2987..5083)
                     /locus_tag="PK513_08395"
     CDS             complement(2987..5083)
                     /locus_tag="PK513_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOO82507.1"
                     /translation="MLFLLGNWSLRLVRMRSFTVLAVVCLLVCAMPQSTRAQDTPVPD
                     KKPEKTESFLFFEVFNLFSSDKEEGPPTPESKIESTPQTMPEPKELTLETQLQESKPE
                     PQEQSDSGPGFNVLEFTASLLNFSTTPIPPQKPERIPVKLGVIEVLMQKTREGISKKP
                     ISRVQAELYKEIFEYQRKGDIRAADNMMKEIDDPRLLGHVLFQRYMHPTAYNSTFTEL
                     SGWLDLFADYPGADQVYKLAQARRPDGVNARIQVPGKAYGITRTSEPTMRLGKRYVSS
                     RARSDEDIRMLNDLNRNIFSLVRKGELSQAHEALQANAAILDNVEYDLLRGEIAAGYL
                     YSGNADEAEKLADQSVKRSGLHVPKAGWIAGLVAWRAKRYSAASRHFEVVARSPYASS
                     WTAAAGAYWAARAHMRTGNIKAVSIWLRRGLNQPRTFYGLISTRALGQDFDFNWKVPA
                     FTKEYMDILSEIPAANRAMALVLAGRSDLAQAELIRIRPENDDQHKALLSYASYANLP
                     GLALRAASSAANEQGSFYDAALYPTGPWQPQEGYKIDPALVHAIMRQESRFDPRAKSP
                     SGAKGLMQLMPATAKSVSDQKGARLDHPETNLELGQRYLEELLESSVVEDDLIYLLIA
                     YNAGPGNLAKWKSRWSDVKDPLLFIELIPSGETRAYVERVLSNYWIYRLREGQNTPTL
                     DAIAASQPAKYNAHAL"
     gene            complement(5145..6017)
                     /locus_tag="PK513_08400"
     CDS             complement(5145..6017)
                     /locus_tag="PK513_08400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO82508.1"
                     /translation="MNTTQMQTGAKAALQFYLDHGVDICLSDEPINRMLELKNTGILE
                     SPASSDQPVRQPDFQHSGIPASNIPLGKSAARNEAARRALACVKREELREAITEFDGI
                     GIKKTATNMVFSDGNPDAPVMLIGEAPGADEDRQGKPFVGVSGQLLDRILACIDLDRR
                     AEDAARAVYISNILNWRPPGNRTPAPAEIEVSLPFIERHIQLIQPKLLILCGSVSAKA
                     LLGRDESISRLRKIWHDYLPQTPELQEGAAPIPAIATYHPAYLLRTPAQKKAVWADML
                     LLQARRRELGLIEA"
     gene            6105..>6416
                     /locus_tag="PK513_08405"
     CDS             6105..>6416
                     /locus_tag="PK513_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO82509.1"
                     /translation="MLDSLRSDRENMEYDVVIVGAGPAGLACAIRLKQLDTDLSVCIL
                     EKGSEVGAHLLSGAVFETRALDELIPDWAEKNAPLHTPATNDAFLFLTPDKAYPFPTP
                     PQ"
BASE COUNT         1409 a         1583 c         1805 g         1619 t
ORIGIN      
        1 gcctcgaggc ggcgcacgcc tgcggacagg gcgctttcgg aaatgatttt gaacgggccg
       61 atctcgccgg tttttttgac gtgggtgccg ccgcagagtt cgatggagaa ttcgcggttt
      121 ttgtcttttg tgcccatggc aacaacgcga acctcgctgt catatttctc accgaacagc
      181 gccatcgcgc cggtttcacg agcttcatca aggcccatca ggcgggtcgt aacaggcgta
      241 tcggctttga tctcggcggc gacgatttct tcgacgcgag cgatttcttc gggcttgatc
      301 gctttagggt gggaaatgtc gaaacgggtg cgctgcgcat cctgcagcga gcctttctgt
      361 gcgacgtgat cgccgagcac gcggcgcaga gcctcgtgca tcaggtgggt ggccgaatgg
      421 ttggcgcgga tggcagagcg gcgcatatgg ttgacttcca tctgcacatt ttcaccgacc
      481 ttcacatcat tggcggtttg cgccctatgc gcgaaaatgc tgccaacaaa ttttttggta
      541 tcgatgactt caatatcgcc attcgtatgg gttaggttac ccttgtcgcc gacctgcccg
      601 ccgctttcgg cgtagaacgg agtctggttg gtgatgatgt agccttcttc accggatttg
      661 agcatttcgg cgcggtggcc gtctttgacg atggctaaaa tctggccttc ggcggcttca
      721 acctcatagc cgaggaattc ggtcggaccg aaatcgtcga gaatatcata ccagattttt
      781 tcggtggcgg catcgccgga gccggaccac gcggcgcggg ctttggcttt ttgctcttcc
      841 atgcaggtgt taaagccgtc ttcatcgact tcgatattct ggcttttgag tgcgtcctgg
      901 gtaaggtcga gcggaaaacc gtaagtgtcg tagagcttga aggcgacatc gcctgggaat
      961 tttttaccgc tggagacttt ttcggtctct tcctcaagca ttttaaggcc gcgatcgaga
     1021 gtgagtttaa agcgctcttc ctcggatttg agggtttcga cgatcaggct ttcggcgcgg
     1081 ccaagctccg gataggcctc gcccattttc tccagcagcg tggggaagag tttgcacatc
     1141 aaaggatctt gtgcgcccag caagtgcgca tggcgcatac cgcggcgcat aatccggcgc
     1201 agaacataac cgcgtccttc gttcgagggc atgacgccat cggccatcag gaaggccgag
     1261 cagcggatgt gatcggcgat gacgcggtga gatgcgctca tatcgccatc gggatcaacg
     1321 cccgtcaagt cggcggagtg ttcaataagg ccgcgcataa tgtcgatggc atagttatcg
     1381 tgagagccca tcaaggttgc ggcaatgcgc tcaaggccca tgccggtatc gacgctttgg
     1441 gcgggcagat cgatgcgcgt atcggcatcg acttgctcgt attgcatgaa gacattgttc
     1501 cagatctcga taaagcggtc gccatcttct tccggggagc ccggcggacc gccccagata
     1561 tgatcaccgt gatcatagaa aatttcagtg cacgggccgc acgggccggt atcacccatg
     1621 cgccagaagt tatcgtcggt ggggatgcgg atgatttttt catcagccag ccctgaaacc
     1681 tttttccaga tggcggcggc ttcttcatcg gaagagtgga cggtgacaca gagtttttcg
     1741 gcgggaaggg cgaagttttt ggtgaccagt tcccaggcaa aagcaatggc gtcgtctttg
     1801 aaataatcgc cgaaggaaaa attgccgagc atttcgaaaa aagtatggtg gcgcgcggta
     1861 tagccaacat tatcgaggtc gttgtgcttt ccgccggcgc ggacgcattt ttggctggtc
     1921 gtcgcgcggg tgtaggggcg cttgtccttg ccggtgaaca cgtccttaaa ctggaccatc
     1981 ccggaattca cgaacatcaa tgtggggtcg ttttgcggga ccagaggcga ggattcgacg
     2041 accgtgtggc ccttcccttt gaagaaactt aaaaattcgc tgcggatgtc attgacagtt
     2101 ttcatagcct gcgctctatt tccttattga gtgaaggagt atttatgaac gaatttgtaa
     2161 cgcttgccaa gaggttggct gatgaggctg gcaaaattgt taagcgctat taccgccaac
     2221 cgtttgatgt gatcagcaaa gaagacgaaa gtccggtgac gattgccgat cgcaagattg
     2281 aggagcggat gcgcgagatc cttgaagatg cgcgtccgca ggatgggatt tatggcgaag
     2341 agcacggcgt taagactggt gagagcgggc ttacgtgggt tctggacccg attgacggga
     2401 caaagagttt tgtgattggg cgcccgactt tcgggacttt gattgctttg tgcgaggata
     2461 gcatacccaa gctaggggtg attgaccaac cgatccttgg tgaacgatgg attggtgatg
     2521 gtgaacagac tttttttaat ggaaggcccg ttcaatgtgc gccttgtgcc tccatcgaga
     2581 ctgcccggat cggcagcacg accccggcga tgtttgaagc aaccggcccg gtttataaaa
     2641 atttcaatga agggcggttt ttctggggcg gagattgcta tcaatacgga ctgatggcct
     2701 gcggttttgt tgatattatt ctggaagccg atatgaagcc gtatgattat gcggcgctgg
     2761 tgcccatagt acagggtgca ggcgggcata tctctgattt tagcggtgcg gcattgatat
     2821 tagagagtga tggcagcgtt gtggcctgcg gcgatcccgg cctatggccc gagatgcata
     2881 aacgcctgtc agcgtaaatt aagagcgtca ttgcgagcgc cagcgaaaca atccaaaata
     2941 gattgccgcg tcgggctcaa cgccctcctc gcaatgacaa tatgttttac aatgcatgcg
     3001 cgttatattt cgcaggctgg ctggcggcga tggcgtccag tgttggggtg ttttgtcctt
     3061 cgcgcaggcg gtagatccag tagttggaca gcactcgttc gacataggcg cgtgtttcac
     3121 cagaaggaat tagttcaata aacagcagcg ggtctttgac gtctgaccag cggctcttcc
     3181 attttgccag attgcccgga ccggcattat aggcgatcaa gagataaatc aggtcatctt
     3241 caacaacgct cgattccagc aactcttcca aataacgctg tcccagttcc agattggttt
     3301 cgggatgatc gagccttgcg cctttctgat cggaaacgga tttggccgtg gcgggcataa
     3361 gctgcatcaa gccttttgcg ccgctggggc tttttgcgcg cggatcaaag cgtgattcct
     3421 ggcgcataat tgcatggacc aacgccggat cgatcttgta gccttcctga ggctgccagg
     3481 gcccggtcgg ataaagtgcg gcgtcataaa aactgccctg ctcattggcc gcagaactgg
     3541 cagcgcgcaa cgcaaggcca ggaagatttg cgtaagacgc gtaagacagc agcgctttgt
     3601 gctgatcatc attttccgga cggatgcgga taagctcggc ctgggccaga tcggagcgtc
     3661 ctgccagcac cagcgccatt gcgcggttgg ccgccggaat ttcgcttaaa atatccatgt
     3721 attccttggt aaaagccgga actttccagt tgaaatcaaa atcctgtccc agagcgcgcg
     3781 tagaaatcag gccgtagaat gtacgcggct ggtttagccc gcggcgcagc caaatgctta
     3841 cggccttgat attgcccgta cgcatatgag cgcgggcggc ccaatacgcg ccagcagcag
     3901 ctgtccagct tgaggcatag ggcgaacgcg caaccacttc aaaatggcgc gaagcagcgc
     3961 tatatcgctt agcgcgccaa gccaccaatc cggcaatcca gcctgccttc ggcacatgca
     4021 gaccagaacg ctttacgctt tgatctgcta atttttccgc ctcatcggcg ttaccactat
     4081 agagatagcc agcagcaatt tcaccgcgca ataaatcata ttcgacatta tccaaaatag
     4141 cggcattggc ttgcaaggct tcatgggcct gtgatagctc tccttttctg accaatgaga
     4201 aaatattgcg gttcagatcg ttaagcatgc gaatatcttc atcactgcgc gcgcgcgatg
     4261 aaacatatct tttccccagg cgcatggtcg gctcgcttgt acgcgtaata ccgtatgcct
     4321 tgccgggaac ttgaatcctt gcatttacgc cgtcaggcct gcgcgcttgc gccaatttgt
     4381 aaacctgatc tgcgccggga taatcggcga acagatccag ccaaccgctc agctctgtaa
     4441 aagtagagtt ataggctgta gggtgcatgt agcgttgaaa tagaacatga ccaagcaacc
     4501 ttggatcgtc tatttctttc atcatgttat cagctgccct gatgtcgcct ttacgctgat
     4561 attcaaaaat ttctttgtat aattcggcct gtactctgct tatgggtttt ttcgatatac
     4621 cttcacgggt tttttgcatc agcacttcta ttacgccaag tttgaccgga atcctttcag
     4681 gtttttgcgg cgggatcggc gttgttgaaa aattcagcaa agacgccgta aactccagaa
     4741 cattgaaacc aggcccagag tctgattgtt cttgtggttc cggttttgat tcttgcagct
     4801 gcgtttccag agttagctcc ttgggttccg gcatcgtttg cggtgttgat tcgattttgg
     4861 attctggagt tggcggccct tcttccttgt ctgaagaaaa caggttgaac acttcaaaga
     4921 acagaaaact ttctgttttc tcaggttttt tatccggaac aggtgtgtct tgcgcccgtg
     4981 tactttgagg catagcgcat acaagcaggc atacaaccgc cagcacagta aaactgcgca
     5041 tgcgcaccaa acgcaaactc caatttccga gcaaaaacag catggatgtg aaattacaac
     5101 aatggctgag gagtgtctat aattacaaaa gctaatgtga taaatcaggc ttctatgagg
     5161 cctaattcac ggcgccgtgc ttgcagtaag agcatatcag cccaaacagc ttttttttgt
     5221 gcgggtgtgc gcagcaaata ggccggatga taagttgcaa tggcggggat gggggccgcg
     5281 ccttcttgca attcgggagt ttgaggcaaa tagtcatgcc agatctttcg caggcgtgaa
     5341 atgctttcat cgcggcccag gagcgctttg gctgaaacgc tgccacacag aatgagcaat
     5401 ttaggttgta taagctggat gtgacgttcg ataaaaggca ggctaacctc aatttctgcc
     5461 ggggcaggcg tgcggttgcc cggcgggcgc caattgagga tgttggagat atagacggcc
     5521 cgcgcggcat cttcggcgcg gcggtccaaa tcgatgcacg ccagaatgcg gtcaagcaat
     5581 tgcccgctga cgccaacaaa aggtttgccc tgacgatcct catcggcgcc gggggcttcg
     5641 ccgatcagca tgacaggcgc atcggggttg ccatcggaaa acaccatatt ggtagcggtc
     5701 ttcttgatac caattccgtc gaattcggta atcgcttcac gcaactcttc gcgcttcacg
     5761 caagccagcg cacgcctggc tgcttcgttg cgcgcagcag atttgccaag cggaatattg
     5821 gatgctggaa tgccggagtg ttggaaatcg ggctgccgga caggctgatc gctcgatgct
     5881 ggtgattcca atattccggt attctttaat tccaacattc tgtttatcgg ctcgtcagat
     5941 aaacatatat ctaccccgtg atcgaggtag aattggagtg ccgctttggc gccagtttgc
     6001 atttgagtgg tattcattgg cttttgccct taaaagtcta tatttatgct tgaggtttta
     6061 ggttaaaata taagttcaat atatgaaaga ggttcatctc atttatgctc gattctctgc
     6121 gctctgatcg cgaaaatatg gaatatgacg ttgtgatagt aggtgcgggt ccggcggggc
     6181 tggcttgcgc tattcgattg aaacaattgg atacggattt gagtgtttgt attcttgaaa
     6241 aagggtcaga ggtcggagca catctgcttt cgggcgcggt ctttgaaacg cgggcgctag
     6301 atgagcttat tcctgattgg gctgagaaaa atgcgccgct gcatacgccg gcgaccaacg
     6361 atgccttcct gtttcttacg ccggataagg cttacccctt cccgacgcca ccgcag
//
LOCUS       DAOSWR010000117         6335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8073_length_6335_cov_5.271815, whole genome shotgun sequence.
ACCESSION   DAOSWR010000117 DAOSWR010000000
VERSION     DAOSWR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8073_length_6335_cov_5.271815"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..647)
                     /locus_tag="PK513_08410"
     CDS             complement(204..647)
                     /locus_tag="PK513_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82510.1"
                     /translation="MGQENLRETFAMRLIDIKPDKKHVVISMPQQILNDIFLIIGAAK
                     DKNDYALDDAVGMNDTPPTGSFDELIAELRNNNNDGTNEPVHITLTEKNLRHLSMLLE
                     TISDVMCMEDHFPSLLEKGITDEKAEEMVDQMWKIRNEAIPSPPT"
     gene            complement(721..2580)
                     /locus_tag="PK513_08415"
     CDS             complement(721..2580)
                     /locus_tag="PK513_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HOO82511.1"
                     /translation="MNNNPTLDNETFKQITAAALRTLAGEKDLEVTYSAAEKPVGKAP
                     LGGHPRLPEPGHNLPPAEKRLLRGCADAQALYIAHHDEALHRRLSLRDAQAQEAFNAL
                     EQARCEALGMARMRGVAGNLSAVLDEKCKRAGFDTLTSKDQAQLGDALHVLARLALSG
                     EDAPHSADTLVKLWEPTLQSSQVQTTLTVLKDNLHDQRVFAAQAMQLLETLDMPVDGR
                     GDNPGEDGEETESAPQADQPQDQEQDEDGEEQAGEESSAEAGMDDTYEGDDKPQDIDG
                     PGDDMGEMNDVPDDYGQEQSDPALQKRKDFLEGARGRYHVYTTQFDEEIDAAELADPG
                     ELTRLRQMLDTQLSAHQAIITKLANRLQRKVMAQQRRSWEFGLDEGILDPSRLARIIA
                     NPTVPVQYKAEKETNFRDTVVTILIDNSGSMRGRPIALAAMSADIISRTLERCGVGSE
                     ILGFTTRAWKGGKARELWMDQGRPPEPGRLNDIRHIIYKAADAPMRRTRKNLGLMLKE
                     GILKENIDGEALVWAYNRLARRPEARKILMVISDGAPVDDSTLSVNPSNILEADLRNV
                     IGWIEEKSDIEISAIGIGHDVTRYYTRAMTIADADELAKALIGQLEGLFEESK"
     gene            2579..3058
                     /locus_tag="PK513_08420"
     CDS             2579..3058
                     /locus_tag="PK513_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82512.1"
                     /translation="MSVFTTTSLRGEASVSTKQSSAVLWIASGAIHPRNDKGGKILHF
                     FIIACYRKFGETKMTKEKMFRVIGAKGEIPWELIAPHERQAQINHGQSLERLNGRGGL
                     NPEEMVAVLEDRPWRQMNSKEAINRINELWDEFEARKKPAHDFLGRSPTGSTTDGPA"
     gene            complement(3055..4068)
                     /locus_tag="PK513_08425"
     CDS             complement(3055..4068)
                     /locus_tag="PK513_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO82513.1"
                     /translation="MSFDITTIKAKEGKYSSIPNIKVNVRDVFGLDIDWQVPAFSEDS
                     ANVPAIDKSYQFDHDTTLAILAGFAHNRRVMVQGYHGTGKSTHIEQVAARLNWPCVRI
                     NLDSHVSRVDLIGKDTIVLREGKQITEWKEGLLPWSIQNPVALVFDEYDAGRPDVMFV
                     IQRILEQEGKLTLIDQNRVIRPHPAFRLFATANTVGLGDTSGLYHGTQQINQAQMDRW
                     NICVTLNYLPHDDEMNIMLAKFPELKTGEGRDMVDKMVRMADLTREGFINGDLSTLMS
                     PRTVLAWIENTLIFDGNRDFAFQLSFMNRCDEAERPLIAEYYQRCFDVELPASAIRNV
                     AVS"
     gene            complement(4118..4723)
                     /locus_tag="PK513_08430"
     CDS             complement(4118..4723)
                     /locus_tag="PK513_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOO82514.1"
                     /translation="MPKISLRPKSPEFQDHQAQTRHHRCDMPGCREEGGFRAPKDRSL
                     STHYWFCQDHIREYNKAWNFFEGMAACEVEEHMINSLYGDRPTWQYGVNGDAQEALYR
                     KAWQMYNGDNEPPPRSKDRARKDIPSALTPEYEALVIMGLEPPVTLSAIKARYKLLAK
                     KHHPDLNKGCLKSEELLKQINMAYTILKLAYEKFENLPERN"
     gene            4818..5078
                     /locus_tag="PK513_08435"
     CDS             4818..5078
                     /locus_tag="PK513_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOO82515.1"
                     /translation="MHMDELIKRLVEENLHPTHMELINESHKHADHAGDNGSGQTHYK
                     LIIVSEAYKGLSRIECHRITMVILDEAFKDGLHALSLKLSTP"
     gene            5180..5644
                     /locus_tag="PK513_08440"
     CDS             5180..5644
                     /locus_tag="PK513_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOO82516.1"
                     /translation="MQSSFVRSNVTEERTLNSEAKSTLLSRNITVAGRRTSVRLEPEM
                     WMALKEISKREKCTMHDICSLINARKNQNTSLTAAIRVFLMLYFRASSTEDGHSRAGH
                     GDFEHMKIRAKIMIKPAANAMGQGGKNIDYERIQLETNKIPDEDSRHMERAL"
     gene            complement(5677..>6335)
                     /gene="accA"
                     /locus_tag="PK513_08445"
     CDS             complement(5677..>6335)
                     /gene="accA"
                     /locus_tag="PK513_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011748623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyltransferase subunit alpha"
                     /protein_id="HOO82517.1"
                     /translation="GLGRFQGRPCVILGQEKGHDTESRIYHSFGMARPEGYRKAQRLM
                     AMASQFQLPVLTFIDTAGAYPGMGAEERGQSEAIAKSIESCLRTDVPIISVVIGEGGS
                     GGAVAIAVADRIYMLEHSIYSVISPEGCASILWRSGDYAQEAASALKLTAQDLYELKL
                     IDAIIAEPLGGAHRHHEETTASVGRQLQQGLKELTPWDGAELIKARRQKFLAFGRESL
                     "
BASE COUNT         1574 a         1632 c         1497 g         1632 t
ORIGIN      
        1 ttgattttct tgccaaagtt tactggaagg aaaatattgt gtggtcgccg ggctacttcg
       61 taagcagcgt aggcgttgat gagcacgtta ttcgccgtta tgtagaacgt caaggacacc
      121 gggattcagg tcaattccgc gaggaattgt aatgcgcaaa gcgcggcgca gccccataga
      181 accacgggtt tacccgtggg tatctatgtt gggggtgagg ggatcgcttc attgcgaatt
      241 ttccacattt gatccaccat ctcttcagcc ttttcatctg tgatgccttt ttcaagcaat
      301 gaagggaagt gatcttccat acacattaca tctgaaatgg tttctaaaag catggaaagg
      361 tgccggagat ttttttctgt cagggtaatg tgaacaggtt catttgtacc atcattgttg
      421 ttgtttcgca gctctgcaat gagttcgtca aaagaaccgg tagggggggt atcgttcatc
      481 cccacagcat catctaacgc atagtcattc ttgtctttgg cagcaccgat gatcagaaaa
      541 atgtcattaa gtatttgttg tggcatagaa atcaccacat gctttttgtc tggtttaata
      601 tctataagcc gcattgcgaa tgtttctctt agattttctt gtcccatttt tgaccctcgc
      661 gtctattcta aggcatagaa taccaaaaaa ccgttaatga aggaaataac cgggcagggg
      721 ctatttgctc tcctcaaaca acccttcaag ctggccgatc agcgctttgg ccagttcatc
      781 cgcatcggca attgtcatcg ctctggtata atagcgtgtt acgtcatggc caatccctat
      841 cgcgctaatt tcaatatcac ttttctcctc aatccagccg atcacattgc gcaaatccgc
      901 ttcgagaata ttcgatggat tcaccgacaa ggtcgaatca tcgaccggcg cgccgtcgga
      961 aatcaccatc aaaatcttgc gcgcctcagg cctgcgcgcc agccggttat acgcccagac
     1021 cagggcttcg ccatcaatat tctcttttaa gatgccctct ttcagcatca gcccgagatt
     1081 tttacgcgtc cggcgcatcg gcgcatcggc cgccttatag atgatatggc gaatatcatt
     1141 cagccgcccc ggttccggtg gacggccctg atccatccat aattcccgcg ctttaccgcc
     1201 tttccacgcc cgtgtcgtaa aaccgaggat ttcagaaccc accccgcaac gctctaacgt
     1261 gcgtgaaata atatctgcac tcatagccgc cagcgcaatc gggcgcccgc gcatagagcc
     1321 ggagttgtcg atcaggattg taacaacggt atcacgaaaa tttgtctctt tctcagcttt
     1381 gtattgcacc ggcacagtcg gattggcaat aatgcgtgcc agccgcgacg gatcaagaat
     1441 gccttcatcc agtccaaact cccaacttcg gcgctgttgc gccattacct tgcgttgcag
     1501 ccggttggct agcttggtga taatcgcctg atgcgcgctg agctgcgtat cgagcatctg
     1561 gcgcaggcgc gtaagctcac ccggatcagc cagttccgct gcatcaattt cttcatcgaa
     1621 ctgcgtagta taaacatggt aacgtccgcg cgcgccttcc agaaaatctt tgcgcttttg
     1681 caaggctgga tcgctttgct cctgcccgta atcatcgggc acgtcattca tctcgcccat
     1741 gtcatcgccg gggccatcga tatcctgcgg cttatcgtca ccttcatagg tatcatccat
     1801 accggcctcg gcgctggact cctcacccgc ttgttcctcg ccgtcttcat cctgttcctg
     1861 atcttgcggt tgatcggctt ggggcgcact ttcggtttct tcgccgtctt caccgggatt
     1921 atcaccgcgc ccatccaccg gcatatcgag agtttccaaa agctgcatcg cttgcgcggc
     1981 aaagacacgc tgatcatgaa gattgtcttt cagtactgtg agcgttgtct gcacttgcga
     2041 cgattgtagg gttggctccc aaagcttcac caatgtatcc gcagaatgcg gtgcatcttc
     2101 accgctgagc gccagccgcg ccagcacatg cagcgcatca ccgagctggg cttggtcttt
     2161 actggtcagc gtatcaaacc cggcgcgttt gcatttttca tccaaaacag ccgacaaatt
     2221 gcctgccacc ccgcgcatcc gtgccatccc cagcgcctcg catcgcgcct gttccagcgc
     2281 gttaaatgct tcttgtgcct gtgcatcacg gaggctaagg cggcggtgta gagcctcatc
     2341 atgatgggcg atatacagcg cctgcgcatc cgcacacccg cgcaatagac gcttttccgc
     2401 tggcggcaga ttatggcccg gctccggaag acggggatgc ccgcccagcg gcgctttacc
     2461 gacaggtttc tccgctgcgg aataggtgac ctctaaatcc ttttccccgg ccagtgtgcg
     2521 caaagccgcc gctgtgattt gtttaaaggt ttcgttatcg agtgtcgggt tgttattcat
     2581 gagtgttttt accacaacgt cattgcgagg agaagcttca gtgtcgacga agcaatccag
     2641 tgcggtgttg tggattgctt cgggtgctat acaccctcgc aatgacaaag ggggcaaaat
     2701 acttcatttt ttcataattg cgtgttaccg taaatttgga gaaacaaaaa tgaccaaaga
     2761 aaagatgttc cgggttatag gtgctaaagg tgaaatacct tgggaattaa ttgcacccca
     2821 tgaaagacag gcgcaaataa atcacggcca atccttagag cgactgaatg gtagaggtgg
     2881 tttgaatcct gaagaaatgg ttgctgtgct ggaggatcgg ccttggagac aaatgaactc
     2941 aaaagaagcg atcaatcgga taaatgaact ctgggatgag tttgaagcga ggaaaaagcc
     3001 agcccacgat tttcttggaa gatcgcctac tggttccacg acagacgggc cagcctaact
     3061 caccgccaca ttccgtatcg cactggcggg caattcaaca tcaaaacagc gctggtaata
     3121 ctctgcaatc aaagggcgct cggcttcgtc acagcggttc atgaaggaaa gctgaaacgc
     3181 aaaatcgcga ttgccgtcaa aaatcaaagt gttttcaatc caggccagca cggtgcgcgg
     3241 gctcatcaat gttgatagat cgccgtttat aaagccctcg cgggtaagat cagccatgcg
     3301 caccatttta tcgaccatat cacggccttc accggtttta agctcaggga atttggccag
     3361 cataatgttc atctcatcat catggggcag gtaattcagc gttacacaga tattccagcg
     3421 gtccatctgc gcttggttga tttgctgcgt cccgtgataa agcccggacg tatcgccaag
     3481 ccccaccgtg ttggctgtcg caaacagtcg aaaagccggg tgagggcgga tcacgcggtt
     3541 ctggtcgatc agggtcagct ttccttcttg ctccagaata cgctggatca cgaacatcac
     3601 atccgggcgc cccgcatcat attcgtcaaa caccagcgcc acagggtttt gaatcgacca
     3661 cggcagcagg ccttctttcc attcggtaat ctgtttgcct tctctcaaaa cgatggtgtc
     3721 tttgccgatt aaatcgaccc ggctgacatg cgaatcgagg ttgatcctca cacacggcca
     3781 gttcagccgt gccgcaacct gttcaatatg tgtggatttc cccgtcccgt gatacccttg
     3841 aaccattacg cggcggttat gggcaaaacc tgccaaaatc gccagcgtcg tatcgtgatc
     3901 aaactgatag ctcttatcaa tcgccggtac attggcgcta tcctcgctaa atgccggaac
     3961 ttgccaatca atatcaaggc cgaatacatc acgcacatta accttaatat tcggaattga
     4021 ggaatatttg ccctctttgg ccttgatcgt cgtgatgtcg aacgacatcc cggcggcttc
     4081 tttttcctta tccagttctt ctactgattc catgatgtta atttctttcc ggcaaattct
     4141 caaatttctc ataggcgagt ttaagaatgg tataggccat attgatctgt ttgagaagct
     4201 cttcggattt caagcagccc ttattcaaat ccgggtggtg ctttttcgcc agcagtttat
     4261 agcgcgcctt aatcgcgctc agcgtaacgg gaggctccag ccccataatc accaaagctt
     4321 catattccgg ggtgagggcg ctgggtatgt ctttgcgtgc gcggtccttg ctacgtggtg
     4381 gcggttcgtt atcgccgtta tacatttgcc aggccttacg atataaagcc tcctgcgcat
     4441 caccattaac cccatactgc caggtaggcc gatcgccata caggctattg atcatgtgtt
     4501 cttcaacttc gcatgcagcc ataccttcaa aaaaattcca agccttgttg tattcgcgga
     4561 tatgatcctg acaaaaccag taatgcgttg ataaggaccg gtctttgggt gcacggaaac
     4621 caccttcctc tcggcatccg ggcatatcac agcgatgatg acgcgtctga gcctgatggt
     4681 cttgaaattc aggcgatttg ggccgtaaag aaatttttgg cattaaagtc tatcaataat
     4741 gtaaatgtta accctatata atcatgatac agaatttctt cacaaagtgc catgaaaggg
     4801 caggtaagga ctaagttatg catatggatg aattgatcaa gcgtcttgtt gaagaaaatt
     4861 tacaccccac ccatatggag ttaatcaacg aaagccataa acatgcagac cacgccggtg
     4921 ataatggttc aggtcaaaca cactataaac tcataattgt gtctgaggcc tataagggtt
     4981 tgtccagaat agagtgccat agaataacga tggttatatt agatgaagct tttaaagatg
     5041 gattacacgc gctatcccta aagttatcca ccccttgaat aaaatagcaa aaaatatatt
     5101 tgcacccaga agttgcattg atatatacca tagggtgtat taagtgacgt aatgcaattt
     5161 gaaaaatatg ggggatgtta tgcaaagctc ttttgttaga tcaaacgtta cggaagaacg
     5221 tacgctcaac agtgaggcta aaagcacgtt gttgagccgc aatatcacag tagcaggacg
     5281 gcgtacatcc gtacgtctgg agccggaaat gtggatggcg cttaaagaaa tttcaaaacg
     5341 tgaaaaatgc accatgcacg atatttgcag tttaattaat gctcgcaaaa atcaaaatac
     5401 ttcattaact gcggcaattc gggtgtttct gatgctctat tttcgtgcat catccacgga
     5461 agatgggcat agccgtgctg gacatggcga ttttgaacat atgaaaatac gtgcaaaaat
     5521 tatgatcaaa cccgcagcga atgccatggg gcagggaggc aagaatattg attatgaaag
     5581 aatacagctt gaaacgaata aaatacctga tgaggatagc cgccacatgg agcgggcgct
     5641 atagcgcctt gtagggattc acaaaattaa gctgaattat aaggattcac gcccgaacgc
     5701 aaggaatttc tggcgtctgg ctttgatcag ttcggcgcca tcccagggtg taagctcttt
     5761 caagccttgc tgcagttgtc gtccaacgct ggctgttgtt tcttcgtgat gccgatgtgc
     5821 gccgcctagc ggttctgcaa taatagcgtc aatcaacttc agttcataca aatcttgtgc
     5881 cgtaagtttg agtgcgctgg ctgcttcttg agcataatca ccgctccgcc aaagaataga
     5941 agcacaccct tccggagaaa taacggaata aatagaatgt tccagcatgt aaatcctgtc
     6001 tgccacggca atggccaccg cgccgccgga acccccttca ccaatcacca cagatatgat
     6061 cggcacatcg gtgcgcaggc atgattcaat cgacttggca atcgcctcgg attgaccgcg
     6121 ttcctctgcg cccatacccg gatatgcgcc agctgtgtca atgaacgtca aaacaggaag
     6181 ctggaattgt gaagccatag ccataaggcg ctgggcttta cgatagcctt ccggacgcgc
     6241 catgccaaaa ctatgataaa tacggctttc ggtatcatgg cctttttctt gtcctaaaat
     6301 tacacaaggc cgcccctgaa aacgtcccag ccccc
//
LOCUS       DAOSWR010000118         6330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8078_length_6330_cov_5.623108, whole genome shotgun sequence.
ACCESSION   DAOSWR010000118 DAOSWR010000000
VERSION     DAOSWR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8078_length_6330_cov_5.623108"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PK513_08450"
     CDS             complement(<1..1134)
                     /locus_tag="PK513_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO82518.1"
                     /translation="MQEIRSLNPFPRIVKGCSICPDIRAAGHGLLTAIAVQRSEAFLW
                     GPVCIGCGIIVYFMLEYEPPALLGVATVFALLGFYRFVRPFEILRFVCLALLCGACGF
                     SAAAYRTQSVYTPILQKKMAPVQVSGTVESLEYFPNEKSTRAVLGAVRIEKLAEDETP
                     RRVRISFRNGEGLSVGAHIEVLAGLNPPSSPVLPGGFDFQRYMFFKGIGAVGFAYGTP
                     RRVEERGSGGLVARLQALRARIALNAIQKNGENGGVAAALLVGQHTAITESDQEAMRW
                     AGLAHMLAISGLHIGLFFGFVFFVVRFGLALSPRLALRYPIKKYAALCAMFAAVCYMF
                     LAGAGVPTQRAVLMTGVVFAAILLDRSPISMRLVAFAAIVILLI"
     gene            1206..2615
                     /gene="gltX"
                     /locus_tag="PK513_08455"
     CDS             1206..2615
                     /gene="gltX"
                     /locus_tag="PK513_08455"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOO82519.1"
                     /translation="MSIVTRFPPSPTGFMHIGTARTALFNWLYARKNGGKMLFRIEDT
                     DRARHNEEAVVALINAMKWLELDWDGEIVSQYEQKNRHIEIAEELLDKGLAYRCYCSQ
                     DELEEMRKKAREDGKPTFYDRRWRDKGHEDAPKGIEPTIRIKAPREGKSTIQDHVQGE
                     VTVNNTQLDDFIILRADGTPTYMLSVVVDDHDMGVTHVIRGDDHLNNTFRQNVIYDVL
                     GWEKPQYAHLPLILGPDGTKMSKRHGAASVEEYREMGYLPEAMRNYLLRLGWSHGDDE
                     IISDTQALEWFNLDHIGKAAARFDFDKLESLNAHYINESDPQRLIELCMPFYKQRHNI
                     DPDAQAQMRIMQSADELKSRAKTLLQFTDESAFYAKTAPFDYDEKAGALLGQDNLDVL
                     QALKTTLESLSDFTSENIQSAIKTIAADLRESKMGKVAMPLRAALTGTTVSPSIFHAT
                     EILGKTETCARIQNAIDRA"
     gene            2711..4021
                     /locus_tag="PK513_08460"
     CDS             2711..4021
                     /locus_tag="PK513_08460"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOO82520.1"
                     /translation="MGKEPESQNTFTLTNDETGESYKLPVLDGSTGPSVIDVRKLYAE
                     TGHFTYDPGFTSTGSCESRLTFIDGEKGILMHRGYSIEDLAEHSSYLEVCHALLYGDL
                     PNKKEMEEFERTITHHTMVHEQINYFYRGFRRDAHPMAIMCGTVGALSAFYHDSLDIW
                     DPEQRKISAHRLIAKIPTLAAMTYKYNVGQPFMYPRNDLSYAENFLYMCFGLPAEPYK
                     INPLLADAMNKILILHADHEQNASTSTVRLAGSSQANPFTCISSGIASLWGPAHGGAN
                     QAVLEMLDQIGDKKNIPEFIERAKDKDDPFRLMGFGHRVYKNFDPRAGVLKKSCDDIL
                     ADLGVQDPRLELAMELERIALEDDYFVSRKLFPNVDFYSGIILKAMGFPTSMFTVLFA
                     VARTVGWIAQWKEMIEEPTLRIGRPRQLYTGPVARPYVPMDKRK"
     gene            complement(4033..5190)
                     /gene="lpxB"
                     /locus_tag="PK513_08465"
     CDS             complement(4033..5190)
                     /gene="lpxB"
                     /locus_tag="PK513_08465"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOO82521.1"
                     /translation="MNKSVFFIAGEASGDSLGASLMRGLKKREGDLVKFSGIGGPLME
                     GQGFKSLLPMDDLCVMGLWEVVWQLPRLLRLIQGTVEEIEKCAPDVVVTIDLPDFNFE
                     VAQRLKKRGIFKGKIVHYVAPSVWAWRPGRAKKIAGYFDGLMCLFPFEPAYFTEHGLD
                     AAYVGHPLIEVDTAALDPKGFREDLHIQDGALCLGVLFGSRERELSSLSKPFLETIGA
                     LHEQYENLQLIAPTLPSLEYNVTNLLSGIDVPCNVVKDQNFKWDALASCDVALAASGT
                     VALELSYLGVPHIIGYKAHPLTALILKLVVKTKYAHLANILLNEEVVPEYLQTKCSAG
                     KLTRAMMRLLRYPEEQVKQKQAFARLHEKLQLGAGETPSERAAAYVLRMAA"
     gene            complement(5203..6048)
                     /gene="lpxI"
                     /locus_tag="PK513_08470"
     CDS             complement(5203..6048)
                     /gene="lpxI"
                     /locus_tag="PK513_08470"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593693.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOO82522.1"
                     /translation="MSAAEEKCLEGLSRIGIIAGGGALPRMLIAACQDRGIEPFIVAF
                     EGQTDPETPDGLDHIWVHLGATRKVIRTLRKSGIRDLVMLGSIRRPSLSELKPDFKTV
                     EFFAKEGWKSLGDDSLLRALRRFLEGEGFTLHGAQEFIPELLAPEGVIGRIKPHAAQR
                     RDIARGIEVLKALSRQDVGQAAVVQNGHVLGIEGAEGTDGLIRRCGDLKRKGHGAVLV
                     KLCKDTQDQDLDLPTIGPHTVDNIIQSGFSGLAVHAGKSFISQIDDVREKADSAGVFV
                     IGISL"
     gene            complement(6021..>6330)
                     /locus_tag="PK513_08475"
     CDS             complement(6021..>6330)
                     /locus_tag="PK513_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOO82523.1"
                     /translation="HAIIGGMSGVENDVIPYGRVKGERAHLHGLNLIGLERRGFQKED
                     IRHLQRAYNQLFGDEGTMDQRIESVSENYSGNETVTKIIEFAKGKDKFPLCQPPKKNA
                     "
BASE COUNT         1819 a         1885 c         1239 g         1387 t
ORIGIN      
        1 aatcaacaaa atgacaatcg cggcaaaagc cacaagacgc atcgagatcg ggctacgatc
       61 gagtaaaata gccgcaaata ccactcccgt catcaacaca gcccgctggg tcggcacccc
      121 tgctccggcc aaaaacatat agcaaaccgc tgcaaacatc gcacacagcg ccgcatattt
      181 cttaatggga taacgcagcg ccaaacgggg gctaagcgcc aacccaaaac gcacaacaaa
      241 aaacacaaac ccaaaaaaca gcccgatatg cagccccgaa atcgctaaca tatgcgccaa
      301 ccccgcccag cgcattgcct cctgatcact ttcagtaatc gccgtgtgct gccccaccaa
      361 aagcgccgcc gcaacgccgc cattttcacc gttcttttgt atggcgttca aagcaatccg
      421 cgcgcgcaga gcttgaagcc tcgcaaccaa tccaccgctt cccctctcct caacgcggcg
      481 cggagtacca taggcaaacc cgaccgcgcc aatgcctttg aaaaacatat aacgttgaaa
      541 atcaaaccct cccggaagca ccggcgatga aggcggatta agccctgcca gcacttcaat
      601 atgcgcacca acgcttaacc cctcaccatt cctgaagcta atccgcactc gcctcggcgt
      661 ttcgtcctca gctaatttct caatacgtac agcacccaaa acagcacgcg tgcttttttc
      721 attcggaaaa tattcaagag attcgaccgt tccagacacc tgcaccggtg ccattttttt
      781 ctgcaaaatc ggggtataaa cactctgcgt acggtaagcc gcagcgctaa atccacacgc
      841 accacacaac aaagccaaac agacgaaacg taaaatttca aaaggacgca caaagcggta
      901 gaatcctaac agcgcaaata ccgttgctac ccccaacaaa gccggcggct catactccaa
      961 cataaaataa acaatgatcc cacagcctat acacacaggc ccccacaaaa acgcttcgct
     1021 gcgctgcacc gcaatcgccg tgagcaatcc atgtcccgcc gccctgatat ccgggcatat
     1081 cgagcagcct ttcactatcc gcggaaacgg gttcaatgaa cgaatctctt gcatgagccc
     1141 ccaatgcgct ttaagaccat aattcggttt taaagaaaaa cactcagcat acaagcatat
     1201 tcatcatgag catcgtcact cgttttccgc cttctcccac cggtttcatg catatcggca
     1261 ctgcccgcac cgcgctattt aactggctct acgcccgtaa aaatggcggc aaaatgctgt
     1321 ttcgcattga agacacagac cgcgcccgcc acaatgaaga agcggtcgtt gccttgatta
     1381 acgccatgaa atggctggag ttggactggg atggagaaat cgtttctcaa tacgaacaaa
     1441 aaaaccgtca tatagagatc gctgaagagc tgctggacaa aggcctggcc tatcgctgtt
     1501 actgcagcca ggatgaactc gaagaaatgc gaaaaaaggc ccgcgaagac ggcaaaccaa
     1561 ctttttatga ccgaagatgg cgcgataaag gccatgaaga tgcccccaaa ggcatagagc
     1621 cgaccattcg catcaaagcc ccccgtgaag gtaaatcaac cattcaggac cacgtacagg
     1681 gcgaagtgac tgtcaataat acacaactgg acgatttcat tatcctgcgc gcagatggca
     1741 ctccgaccta tatgctttct gtggttgtag acgatcatga tatgggcgtt acacatgtca
     1801 ttcgcggcga cgaccacctc aacaacacct tccgccagaa cgtcatttac gatgtcctgg
     1861 gttgggaaaa gccgcaatac gctcacctgc cgctaatcct cggacccgat ggcaccaaaa
     1921 tgtccaaacg ccacggcgct gcaagtgtag aggaataccg tgaaatgggc tacctgcctg
     1981 aagccatgcg caattacctg ctacggctgg gctggagcca tggcgatgat gaaattatct
     2041 cagatacgca ggccctcgaa tggttcaatc ttgaccatat cggcaaagct gctgcccgct
     2101 ttgactttga taagctcgaa agcctcaatg cccattacat caatgaatcc gacccacaac
     2161 gtcttataga gctttgcatg cctttttata aacaacgtca caacatcgat cctgatgcgc
     2221 aagcgcagat gcggatcatg caaagcgcag atgaattaaa gtctcgtgcc aagacactac
     2281 tccaattcac tgatgaatca gcattttatg ccaaaactgc tccgtttgat tatgacgaaa
     2341 aagccggcgc gttattgggt caggacaatc tcgacgtgct tcaagcgctc aaaaccaccc
     2401 tcgaatcatt aagcgatttc acatccgaga atattcaatc cgccatcaaa accatcgccg
     2461 ctgatctgcg cgagagcaaa atgggcaaag tcgccatgcc gctgcgcgcc gcactgacag
     2521 gcaccaccgt ttcgccatca attttccatg ctactgaaat tcttggaaaa accgaaacat
     2581 gcgcgcgcat tcaaaacgcg attgatcggg cttgatcaat cgcaaaactt ctctacaata
     2641 atgcccagtt gcacaaccgc aaattctgcc tattctattt tcaatacaat caaacaatat
     2701 ggggatccca atgggtaaag aacctgaaag ccaaaataca ttcacactaa ccaatgatga
     2761 gacgggcgaa agctataagc taccggtact tgatggttca acgggaccga gtgtgatcga
     2821 tgtgcgcaaa ctttatgccg aaacaggtca cttcacctat gaccccggat tcacctcaac
     2881 cggaagctgc gaatctcgcc tgacatttat cgatggtgaa aaaggcattc tcatgcatcg
     2941 cggctacagc atcgaggatc tggccgaaca cagcagctac cttgaagtct gccacgctct
     3001 gctttacggc gacctcccta ataaaaaaga aatggaagaa tttgaacgca ccatcaccca
     3061 tcacacgatg gtgcatgaac agatcaacta tttctatcgc ggatttcgcc gtgatgccca
     3121 cccgatggcc attatgtgcg gcactgtcgg agcactctcg gctttctacc acgactctct
     3181 ggatatttgg gaccccgaac aacgtaaaat ctccgctcac cgcctcattg caaaaattcc
     3241 aacattggcg gcaatgacct acaaatacaa tgttggccag cccttcatgt atccgcgcaa
     3301 cgacctcagc tatgccgaaa actttctcta tatgtgcttt ggtcttccag cagaacccta
     3361 taaaatcaat ccgcttttag ccgacgcgat gaacaaaatt ttaattttgc acgctgacca
     3421 cgaacaaaac gcttccacct caaccgtgcg cctggccgga tcatcccaag ccaatccctt
     3481 tacctgtatc tcttccggta ttgcctcgct gtggggaccc gcgcatggcg gtgcaaatca
     3541 ggctgtgctt gaaatgctcg accaaatcgg cgacaagaaa aatatccctg aatttatcga
     3601 acgagccaag gacaaagacg atccattccg cctcatgggc tttggccacc gcgtttacaa
     3661 aaacttcgac ccgcgcgcag gcgttctcaa aaaatcatgc gatgatatcc ttgccgacct
     3721 aggagttcag gacccgcgtc ttgaactggc tatggaactt gagcgcatcg ctctggaaga
     3781 tgattacttc gtcagccgca aactcttccc taatgtcgat ttctattctg gaattatcct
     3841 caaggctatg ggatttccaa cctcaatgtt taccgtgctg ttcgccgtcg ccagaaccgt
     3901 tggctggatt gcacaatgga aagaaatgat cgaggagcca acattgcgca tcggtcgccc
     3961 gcgccagctt tacaccggcc cggtagcccg tccttacgta ccgatggata aacggaaata
     4021 aaaaaaccgg gatcacgcag ccatgcgcaa aacataggcg gcagctcttt cgctcggcgt
     4081 ttcaccagcc ccaagttgca atttttcatg caaccgggca aacgcctgct tttgctttac
     4141 ttgctcttcc ggatagcgca acagccgcat catcgcgcgc gtaagcttgc cggcgctgca
     4201 tttcgtctgc aaatattccg gcacgacctc ttcattcagc aaaatattag ccaaatgagc
     4261 atatttagtc ttcacaacaa gtttcaaaat caatgccgtc agcggatgcg ccttataacc
     4321 gataatatgc ggcacgccta aataagacag ctcaagcgca accgttcccg atgccgccaa
     4381 cgccacatca cacgatgcca acgcatccca tttaaaattc tgatctttaa caacgttgca
     4441 aggaacatca ataccgctca ataaatttgt aacgttatat tcaagcgacg gcaatgtagg
     4501 cgcaatcaat tgtaaatttt catattgctc atgtaatgcg ccaatagtct ccaaaaatgg
     4561 ttttgacaac gaactcaatt cgcgttcccg gctaccaaaa agcaccccca agcacaacgc
     4621 gccatcttgg atatgaagat cttctcgaaa acctttcgga tccagagccg ccgtatcaac
     4681 ctcgatcaaa ggatgcccta cataagccgc atcaagccca tgctctgtaa aatatgcagg
     4741 ctcaaatgga aacaagcaca tcaaaccgtc aaaatacccg gcaatcttct ttgcccgccc
     4801 aggccgccat gcccaaacac ttggcgcgac ataatgaaca attttacctt taaaaatgcc
     4861 ccgttttttc aaacgctgcg ccacctcaaa attaaaatcc ggcaaatcaa tcgtaacaac
     4921 aacatcaggc gcacattttt caatttcctc aacagtgcct tgaataagcc tgagcagcct
     4981 cggaagctgc caaaccacct cccaaagccc catcacacaa agatcatcca taggaagaag
     5041 actcttaaac ccttgcccct ccatcaatgg gccaccaatc cctgaaaact tgaccaaatc
     5101 tccttcgcgc tttttcaaac cccgcataag cgaggctcca agagaatctc cggaagcctc
     5161 tcccgcgata aaaaatacag acttattcat ctatcaaact ccttaaagac taatccctat
     5221 aacaaacacg cctgcactat cagctttttc gcgcacatca tcaatttgac taataaaact
     5281 cttccccgca tgcacggcca aaccactaaa accagattga ataatattat ctacagtgtg
     5341 tggaccaatc gtcggtaaat caagatcctg atcttgcgta tctttacaaa gcttcacaag
     5401 aactgcacca tgtcctttcc gtttcagatc accacagcgc cggattaaac cgtccgtccc
     5461 ttccgctccc tcaatgccca gaacatgacc attttgcact actgccgcct gcccaacatc
     5521 ctgcctgctc aatgctttaa gaacctcaat tccacgtgca atatcacgcc gctgcgccgc
     5581 atggggcttt atacgcccaa taacaccttc cggtgccaaa agctccggta taaattcttg
     5641 cgccccatgc aatgtaaacc cttcgccttc caaaaaccgc cgtaaggccc gcaacaagct
     5701 atcatcccca agactcttcc agccctcttt ggcaaaaaac tcaacagttt taaaatcagg
     5761 cttcaactca ctcaaggatg gcctgcgaat agaacccagc atcaccaaat cacgaatgcc
     5821 ggatttgcgc agcgtacgta taaccttacg ggtagcgccc aaatgcaccc agatatggtc
     5881 cagcccgtca ggagtctccg gatcagtttg accttcaaat gcaacaatga acggttcaat
     5941 acccctatct tgacacgcag caatcaacat tcgtggcaac gcaccgccac ctgcaataat
     6001 tccaatacgg gacaaaccct ctaggcattt ttcttcggcg gctgacataa tggaaacttg
     6061 tccttgccct tggcaaattc aataattttc gtaacagttt cattgccgga gtaattttca
     6121 ctcacgcttt cgatgcgttg atccatggtc ccttcatcac caaaaagctg attataggct
     6181 cgctgcaaat gacgaatatc ttctttttga aaccctcggc gctcaaggcc aatcaggttc
     6241 agtccgtgca aatgcgcccg ctcacccttg acccgaccat atggaattac atcattttct
     6301 acgcccgaca tgccgccaat aatagcatga
//
LOCUS       DAOSWR010000119         6280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8167_length_6280_cov_5.213173, whole genome shotgun sequence.
ACCESSION   DAOSWR010000119 DAOSWR010000000
VERSION     DAOSWR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_6280_cov_5.213173"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PK513_08480"
     CDS             complement(<1..1271)
                     /locus_tag="PK513_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO82524.1"
                     /translation="MVSTTLLKPWGPIMARKKQQRRQKQTKRGFKNWPRAWLVAGVFG
                     ATLCIMLLSFISIPLALLGLGIFGLMGIIETEAHERSFWQQAASFKFKTLKDRQDALV
                     KDMAKNTRDIEALKCKVRNDDPCEQPQAAVASEDMPSKRAPLPLGLDYKELPRSPRAV
                     KPRSRAPIIPAQNSRENFEDLSDSIVRELVHHALHEERVDVFVQPIQRLPQRQTRFYE
                     MFARIRARPGQYLPASRYMAIAEQDHLDDKVDNLLLLHCLKTLSESAHIKRAAPFFIN
                     VKNTTLKNGAFMKRLLGFVAKNRELAPRLIFEIRQRDFDDMPPALLEIMRGLGRLGCS
                     FSLDHVGSLQIDVADLQHFKVRYVKIDAAQLLAAAQNGGKEFSALHKAKRKLEANGIG
                     VIATKIENEEQMRKLLDFDLHYGQGYLFGKP"
     gene            complement(1222..2298)
                     /locus_tag="PK513_08485"
     CDS             complement(1222..2298)
                     /locus_tag="PK513_08485"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOO82525.1"
                     /translation="MVDVYKLARPFVFLMAPEQAHKAVIHALKAGLVPSAPKIDAPEL
                     EQTLWGLKFPNPVGLAAGFDKNAEVIGPAFKLGFGFVEAGTVTPRPQHGNPKPRIFRD
                     PSSEAVINRMGFPNAGMNAFKANLEKFLSQKDRPRGVVGLNIGMNKSQSDPAKDYSAL
                     IRMLGPMADYMVINISSPNTPGLRDLQSREPLLELLAIVKEERHKACGEHPPPVLVKL
                     APDLDEGQQEELAQVALEAEIDGVILTNTTLERPAGLPSDFAAEKGGLSGQPLGDKST
                     GIVRNFYRLTKGKIPIIGAGGVGNAAQAYEKIKAGASLVQLYSALVYEGPYVASAINR
                     GLLSLLKADGFDNIAQAVGADHGA"
     gene            complement(2308..2859)
                     /gene="pgsA"
                     /locus_tag="PK513_08490"
     CDS             complement(2308..2859)
                     /gene="pgsA"
                     /locus_tag="PK513_08490"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOO82526.1"
                     /translation="MMWTIPNILTIGRMALLPVMVALFFLPFAWAAWTCLGLYIIGAV
                     TDFLDGWIARRFNLQSEFGAMIDPISDKVFVVTIMLMLVAVGRIDGLMVLSVVIIIVR
                     EFTVSGMREYLGPKGVKLPVTRLAKWKTTLQMAALGFLIVGPYVFLATLIGNITLAGA
                     AVLTLITGWGYVKTGMDFIRKMP"
     gene            complement(2951..3652)
                     /locus_tag="PK513_08495"
     CDS             complement(2951..3652)
                     /locus_tag="PK513_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO82527.1"
                     /translation="MRVLLVEDDTSTAKSIELMLKSEGYIIDTTDLGEDGLEIGKIYD
                     YDIIILDLMLPDMDGYDVLKALRDAKVETPILILSGLTELDNKIKGLGYGADDYLTKP
                     FDKRELIARIQAIVRRSQGHSQSIIETGKVKVNLDTRTVEVAGSPLHLTGKEYGIIEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPEVKIIDVFICKLRKKIQDAADGDNYIETVWGRG
                     YVLRDPQAASQAASG"
     gene            complement(3804..4211)
                     /locus_tag="PK513_08500"
     CDS             complement(3804..4211)
                     /locus_tag="PK513_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82528.1"
                     /translation="MDVIIVTDIERAQSGSDGFLNCLKKAFNKRGVVHRMASYDGREY
                     DWITEAPQHAVGVVVADMRALSGGITPLQLLVSSVRNVPVLAISDGSILADAENMAKN
                     AGAKDFMLIEKGAIQIAEKALKIAQQPPTTPAP"
     gene            4329..5663
                     /gene="fliI"
                     /locus_tag="PK513_08505"
     CDS             4329..5663
                     /gene="fliI"
                     /locus_tag="PK513_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOO82529.1"
                     /translation="MDRLAQQMDARIKGLTQTETYGEVTKILGLLVEIVGFGAGLSIG
                     AHVHLRPNKNLNVPCEVVGFKDGHALLMPFGTLDGIGLGCKAYIQDTPPVITPDERWL
                     GRVINGMGEPIDGLGPLPQGGYPVPLKNKPPSPHSRQRLGKQLDLGVRAVNTFLATCQ
                     GQRMGIFSGSGVGKSVLLSMMARYTAADVSVIGLVGERGREVQEFLEDDLGPDGLARS
                     VVIVSTGDEPALMRRQAAYMTLAVSEYFRSQGRQVLTLIDSVTRFAMAQREIGLSAGE
                     PPTSKGYPPTTFGELSRLLERAGPGAPGEGSITGLFSVLVEGDDHNEPISDAVRGIVD
                     GHIVMNREIADRGRYPAINVLKSVSRSMPAILTDEQNEIIKRAKQVVTTYEDMAELIR
                     LGAYRRGSDSTVDEALVLYPQIEEFLTQHKDERTTIDESFARLARILGVGYG"
     gene            5656..5814
                     /locus_tag="PK513_08510"
     CDS             5656..5814
                     /locus_tag="PK513_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82530.1"
                     /translation="MAKKSKRKVKLWRLMALIVLVNLAFFVFYDVQTNTVWGLDKILQ
                     AMGYNHHG"
     gene            5807..6232
                     /locus_tag="PK513_08515"
     CDS             5807..6232
                     /locus_tag="PK513_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HOO82531.1"
                     /translation="MAKLNGLIRVHKHALDQKQKFVAELYRQAEELENQKTTLLNQLD
                     EEREKIQEFGVEMLSYFGPYSEAVKERVIEINEAATVLESRIEIAREDMRAAFAELKK
                     IQLTQQARENEEEKERNKKESGELDEIAIEGYRRQQRDE"
BASE COUNT         1523 a         1734 c         1503 g         1520 t
ORIGIN      
        1 tccggcttgc caaataaata cccttgcccg taatgcaaat caaaatcgag caatttccgc
       61 atttgctctt cgttctctat tttagtcgca atcacgccga tgccattagc ttcaagtttt
      121 cgttttgctt tatggagcgc gctaaattct tttccgccat tttgcgcagc agctagcaat
      181 tgcgccgcgt caatctttac atagcgcact ttgaagtgct gtaaatccgc cacatcgatt
      241 tgcaagctgc cgacatgatc gagcgaaaag ctgcatccga gccttcccag cccgcgcata
      301 atttccagta acgccggagg catatcatca aaatcacgct gacggatttc aaaaataagc
      361 cgtggcgcca gttcgcggtt tttcgcgaca aaaccaagca ggcgtttcat aaacgctcca
      421 ttctttaatg tcgtattttt aacattaata aaaaacggtg cggcgcgttt gatatgtgcg
      481 ctttcgctta aagttttcag gcaatgcagc aacaatagat tatcaacttt atcatcaaga
      541 tgatcctgct cggcaatcgc catataacgg ctggcaggca gatattgacc gggccgtgca
      601 cgaatgcgtg caaacatttc ataaaaacgt gtctggcgtt ggggcaggcg ctgtataggt
      661 tgcacaaaca catcgacgcg ctcttcatgc aaagcatgat ggaccagctc acgcacaatg
      721 ctatcgctca aatcttcaaa attttcgcgg ctattttgtg ccggtatgat tggagcgcgg
      781 ctacgcggtt tgaccgcccg tggagagcga ggcaattctt tgtaatccag accaaggggt
      841 aaaggtgcac gcttgctcgg catatcttcg cttgccacgg cggcttgcgg ttgctcacat
      901 gggtcgtcat tacggacctt gcatttaagc gcttcaatgt cgcgcgtatt ttttgccatg
      961 tcttttacaa gtgcgtcttg tctgtctttc agtgttttaa atttaaacga tgcggcctgt
     1021 tgccaaaagc tgcgctcatg cgcttcggtt tcaattatgc ccatcagccc gaaaatcccc
     1081 agtcccaaga gcgccagcgg gatggaaata aaactcagca gcataatgca gagcgtcgcc
     1141 ccaaacacgc ccgcaaccag ccacgcccgc ggccagttct taaatccgcg ctttgtttgc
     1201 ttttgcctgc gctgttgttt tttacgcgcc atgatcggcc cccacggctt gagcaatgtt
     1261 gtcgaaacca tcagctttca gcagtgataa taacccgcga ttaatagcgc tggctacata
     1321 tggcccttca taaaccaggg cgctgtaaag ctgaaccaga ctcgccccgg cctttatttt
     1381 ctcataggcc tgagcagcat tgcccacacc gcctgcgcca attataggga ttttcccctt
     1441 ggtcaggcga taaaaattgc gcacgatgcc cgtagattta tcgcccaaag gctggccgct
     1501 cagcccgcct ttttcggcgg caaaatcgct gggcaagcca gctggccgct ccagcgtcgt
     1561 attggtcagg atcaccccgt ctatttcagc ttctaacgca acctgcgcca gttcctcctg
     1621 ctgtccctca tccagatcag gcgccagttt caccagcacc ggcggcggat gttcgccgca
     1681 ggctttgtgc cgctcttctt tgacaatggc cagaagctca agcaatggct cccggctttg
     1741 taaatcgcgc aaacccggtg tattgggtga agaaatatta atcaccatat aatcggccat
     1801 cggccccagc atcctgatga gtgcgctgta atccttcgcc gggtcgcttt gacttttatt
     1861 catcccgata ttaaggccga caacgccgcg tggccgatct ttttggctaa ggaatttttc
     1921 aagattggcc ttaaacgcgt tcatcccggc attgggaaac cccatccggt tgatcaccgc
     1981 ttcgctggaa ggatcacgaa aaatgcgcgg cttcgggttg ccgtgctgcg gtctgggtgt
     2041 cactgtgccg gcctcaacaa atccaaaccc aagcttgaag gccggaccaa taacctcggc
     2101 attcttatca aatcctgcgg ccagcccgac cgggttggga aactttaacc cccataaggt
     2161 ctgctccagc tcgggtgcat caatcttcgg cgcagacggg accagtccgg ctttcaaagc
     2221 gtgaattact gccttatgcg cctgttcagg cgccatcaaa aatacaaacg gacgagcaag
     2281 cttataaacg tcaaccatat tactggttta aggcattttt ctgataaaat ccatgcccgt
     2341 cttcacgtat ccccagccgg tgatgagcgt cagcaccgcc gcgcccgcca gcgtgatatt
     2401 tccgatcagt gtcgcaagga aaacatacgg cccgacgatc aaaaaaccca atgccgccat
     2461 ctgcagcgtt gttttccatt tcgccagcct ggtcacggga agcttgacgc cttttggtcc
     2521 aagatattca cgcatccccg aaacggtaaa ttcccgcacg ataataataa cgaccgataa
     2581 aaccatcagc ccgtcgatcc gccccaccgc caccagcatc agcataatgg tgacgacaaa
     2641 aaccttgtcg gaaatcggat cgatcatcgc gccgaattcg ctctgcaggt taaagcgccg
     2701 cgcaatccat ccatcaagaa aatccgtcac cgcgccgatg atataaaggc caaggcacgt
     2761 ccacgccgcc catgcgaacg gcaggaagaa caacgccacc atcaccggca gcaaagccat
     2821 cctgccaatc gtcaatatat tcggaatcgt ccacatcatt ggaacattag aatgttggaa
     2881 ttttggaatg ttagaaaaaa cgaaacatat tcgttccaat attctaagat tccaatgttc
     2941 ctaaattcca tcacccgctc gccgcttggc tggccgcttg cgggtcgcgc agcacatagc
     3001 cgcggcccca gacggtttca atatagttgt caccatcagc cgcatcctga attttcttgc
     3061 gcagcttgca gataaacaca tcgataatct ttacttccgg ctcgtccatc ccgccataaa
     3121 gatggttcag gaacatttcc ttggtcagcg tcgtgccctt acgcaaggat agcagctcaa
     3181 taatcccgta ttccttgccc gtcagatgca aaggcgaacc ggcaacttca accgtgcgcg
     3241 tatccagatt aaccttaacc ttgccggttt cgataatgct ctgtgaatgg ccctgagagc
     3301 gccgtacaat cgcctgaatc cgcgcaatca actcgcgctt gtcaaacggc tttgtcaggt
     3361 agtcatcggc gccataccct aagcctttga ttttattgtc aagctccgtc aggcccgaga
     3421 gaatcaaaat cggtgtttcc accttcgcat cgcgcaacgc cttcaaaaca tcatacccat
     3481 ccatatccgg caacatcaaa tcgagaataa tgatgtcgta atcgtaaatt ttcccgattt
     3541 ccagcccatc ttcacccaaa tcggtcgtat cgatgatata gccttcggat ttcagcatta
     3601 attctatgct tttggcggtt gaggtgtcgt cttctacgag gagtacgcgc atggtattga
     3661 ttcccttgtg tttttcgatc tgttcagttc gttttcaagt tttacgaggg gggcgttgca
     3721 ccatgccgcg ttaaactttc ttaacaacta tattaacaaa tattaacaag gttaacaaat
     3781 tctttatttg ggatggatat tctttacggc gcaggggtgg tgggtggttg ttgtgcaatt
     3841 ttaagagctt tctctgcaat ctgtatagcc cctttttcta tcagcataaa atctttcgct
     3901 ccggcgttct tcgccatatt ttctgcgtca gcaagaatac tgccgtctga aatagctaga
     3961 acgggaacgt tccgaacaga actaacaaga agttgcaacg gagtgatacc acctgacaaa
     4021 gccctcatat cagcaacaac aacaccaacc gcatgttggg gtgcctcagt gatccagtca
     4081 tactccctgc catcatagct tgccatccga tgcacaacgc cccgcttatt aaaggctttt
     4141 ttgaggcaat tcaaaaagcc atctgaaccg ctttgcgcgc gctctatgtc cgtaacaata
     4201 atgacgtcca tgtgaatata tccaaattat taatattatg tataataaaa aacccataac
     4261 caatgcaagc gttttatgag ccgtcatttc cattcgtttt ttgaaaagcg ttctgtatac
     4321 tggaaaccat ggaccgttta gcacagcaaa tggatgcccg catcaagggc ctcacgcaaa
     4381 ccgaaaccta tggcgaggtt acgaagatcc tcggcctgct ggtagaaatc gtaggctttg
     4441 gcgctggcct gtcaatcggt gcgcatgtac atctgcgtcc caataaaaat cttaacgtcc
     4501 cttgcgaggt tgtcggtttc aaagacggcc acgcgctact gatgcctttt ggcacccttg
     4561 atggtattgg gctgggctgc aaagcctata ttcaggatac gcctcccgtc attactccgg
     4621 atgaacgctg gcttggccgg gtgattaacg gtatgggcga gccgattgac ggtctcgggc
     4681 cactgcccca aggcggctat ccggtcccgc tgaaaaacaa accgccgtcg ccccattcgc
     4741 gccagcgcct cggcaagcaa cttgatcttg gcgtgcgtgc ggtcaacacc tttttggcta
     4801 cctgtcaggg ccagcgtatg ggcattttct ccggctccgg cgttggaaaa tccgttctgc
     4861 tctcgatgat ggcgcgctat acggcggccg atgtttccgt gattggcctc gtcggcgagc
     4921 gcgggcgcga ggtgcaggaa ttcctcgaag atgatctcgg tcccgatggc ctcgcgcgct
     4981 cggtcgtgat cgtttcaacc ggtgatgaac cggccctgat gcgccgtcag gctgcctata
     5041 tgacgctggc ggtcagcgag tattttagat cgcaaggccg tcaggtcctc actctgattg
     5101 attctgtcac ccgctttgcc atggcccagc gtgaaatcgg actgagcgca ggagagcctc
     5161 ccacctccaa gggctatccg cccaccactt ttggagaact ttcacggctc ttggaacgcg
     5221 ccgggcccgg agcgccaggc gaaggctcca ttacggggct gttttccgtg ctggtcgagg
     5281 gcgacgatca taacgagcct atctccgatg cggtgcgcgg aatcgtcgat gggcatattg
     5341 tgatgaaccg tgagattgct gaccgtggcc gctacccggc gatcaatgtt ctgaaatccg
     5401 tgtctcgctc gatgcccgcc atcctcaccg acgagcaaaa cgaaatcatc aaacgcgcca
     5461 aacaagtcgt gaccacctac gaagacatgg ctgagctgat ccgcctcggc gcataccggc
     5521 gtggctcgga ttctacggtg gacgaggcgc tggtgctcta tcctcagatc gaagaattcc
     5581 tcacccagca taaagacgag cgtaccacca ttgacgaaag cttcgcccgc ctcgcccgca
     5641 tcttaggggt aggatatggc taagaagagc aaacgtaagg ttaaactatg gcggctgatg
     5701 gcgctgatcg tgctggtcaa tctcgctttt tttgtgtttt atgatgtgca aaccaatacc
     5761 gtatgggggc tagataaaat attacaggcg atggggtata atcaccatgg ctaaactcaa
     5821 cggattaatc cgcgttcata aacacgctct tgatcaaaag caaaaattcg tggccgagct
     5881 gtaccggcag gccgaagagc tggagaatca gaaaaccacg ctgctcaatc aactcgatga
     5941 agagcgagaa aaaatccagg aattcggtgt cgaaatgttg tcttattttg gtccctattc
     6001 tgaagcggtc aaggagcgcg tcatcgagat aaacgaggcc gctacggtcc tggaatcgcg
     6061 catcgaaatc gcccgcgaag atatgcgcgc ggcctttgcc gaactcaaga aaatccaact
     6121 tacgcagcaa gcccgtgaaa acgaggaaga aaaagaacgc aacaaaaaag agtccggcga
     6181 actcgatgaa atcgccattg aagggtatcg gcggcagcaa agggatgaat agcgtcattg
     6241 cgaggagtga ggccttgccg ccgaaggcgg caaggcgaac
//
LOCUS       DAOSWR010000120         6206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8304_length_6206_cov_5.138514, whole genome shotgun sequence.
ACCESSION   DAOSWR010000120 DAOSWR010000000
VERSION     DAOSWR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_6206_cov_5.138514"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1528
                     /gene="glnA"
                     /locus_tag="PK513_08520"
     CDS             101..1528
                     /gene="glnA"
                     /locus_tag="PK513_08520"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOO82532.1"
                     /translation="MTTALKFKNIAEFLKYIKENDIQYVDMNFTDLRGKWQHTTEYAP
                     ELDEYKLNRGIFFDGSSIAGWQPINDSDMVIKPDLGKVAIDPFAAQRTLKVFCDIHEP
                     NSGKPYALDPRSIAKAAEKHLKSSGLADTAYFGPEAEFFIFDDVKIHMETNRVSFEIN
                     SEEAPYNAGRHYDTGNMGHRPQTKGGYFPESPVDHLSDIRGEMLTVIQSMGVEVEKHH
                     HEVAPSQCELGIRFSTLLDSADNIQLYKHAVHNVANAYGKTATFMPKPVFGDNGSGMH
                     VHQSLWKSGKPLFAGDSYANLSENALYYIGGIIKHARSLNAFTNPTTNSYKRLVPGYE
                     APVLLAYSARNRSASCRVPYSASPKGKRIEVRFPDPTANPYLAFAAMLMAGLDGIENK
                     IHPGEAMDENLYDLSQEEQDKLPNVCHSLRSALEALREDHEFLLKGDVFTQSAINAYI
                     GLKEEENKAYEMMIHPIEYKLYYSS"
     gene            complement(1542..2777)
                     /locus_tag="PK513_08525"
     CDS             complement(1542..2777)
                     /locus_tag="PK513_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82533.1"
                     /translation="MCYNNVCVPFIALKESIRGVLEMISLHRICVLLVLVSGISWFFP
                     HSAFAQKVMEQYRKPMPEFDLLSSGEFTENTEPLVLKTFGDPALNFEIRIPKGWENMT
                     KGRPEDFSVTNKIMGEIVRFYGPPDIYSERSYLTVEAMSMDYYMTVEQWLMQFFLSSS
                     YNVQGLTVHDDTRAEALYVFIKNKVSYAVRASVYTNGKHVMYAQYVVPIERWHDEKVM
                     QKMVLDSFDPITPVKEYIEEMQRYQFLDLAEFMYPVSWELKALPIRTIDRMEVLLLNT
                     HYIHDGSHTGKIRLDGQIGIDLVSIYASETLDDELNYYKEDVAEKGLVMGDVIENRNE
                     FFLGDSYDFVDTQVFKVTDENERVIEHELWLTIMSAGDFYYFVSLFTPSRDDDYFKWA
                     RNVETYKLVVNEIKPRTAE"
     gene            2950..4998
                     /gene="parE"
                     /locus_tag="PK513_08530"
     CDS             2950..4998
                     /gene="parE"
                     /locus_tag="PK513_08530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOO82534.1"
                     /translation="MSDLFAPPSAASKNNYDASSIEVLEGLEPVRKRPGMYIGGTDER
                     ALHHLAGEILDNSMDEAVAGHADWIEIALEADYSLIIRDNGRGIPVDEHPKYPGKSAL
                     EVIMTTLHSGGKFSGKSYETSGGLHGVGISVVNALSDNMWVEVAREKKLYRQSFSRGL
                     PTSKLEDLGAINNRRGTTVSFHPDPDIFGSQARFKPGWLFQMARSKAYLYSGVEIRWK
                     CAHEIIPEDSKITPEAVFKFPNGLLDYLDATLHNRKTVIPNPFAGKADLPGNEGRVEF
                     AITWPENGEGFSHSYCNTVPTPQGGTHETGLRTALSRSLRAYGEMIGVKKANIITADD
                     VLGGAAILLSVFIKDPQFQGQTKEKLASSAVSKWVDSAIKDHFDLWLSGDPASATKLL
                     NAIILRAEERLRRKMDRVQARKSATQKLRLPGKLADCSRANAEDTELFIVEGDSAGGS
                     AKQGRNRETQAILPLRGKILNVVSATADKIRANQEIQDLIQALGCGAGRDFHLSKLRY
                     ERVIIMTDADVDGAHIAALLITFFYTQMRELVEEGHLYLAQPPLYRLVAGNNSAYAKD
                     DEHKDALMETKEFKGKKVEVSRFKGLGEMPAKQLKETTMNPASRTLLRVTLPEAAAAL
                     AIGLSGEELESLEGENPLEAGASQADIDDLVERLMGKNAEPRFQFIQDNAEFASDIDV
                     "
     gene            5034..5480
                     /locus_tag="PK513_08535"
     CDS             5034..5480
                     /locus_tag="PK513_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82535.1"
                     /translation="MNWIKRQLYRTIDYGHEAKRRAERRKSLQNFLLIPSVILSTSLI
                     WLLSLYCFSQWHAYIFPEETLANAEGIGPILVTVSPLFFALLFGMILGNKLVALFPTT
                     KRVLEQEAQKFSQTSYKESQKHLLRLSVIIIPLSFGLAIWGVFLPW"
     gene            5570..>6206
                     /locus_tag="PK513_08540"
     CDS             5570..>6206
                     /locus_tag="PK513_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82536.1"
                     /translation="MKRPILILALLIMTGAIGVTGYVWLNIPQHLETRLRQTMNEAGF
                     ENYTLPPAEKKLGQTVYQNIALDPDGFSTLENLTITYNPLKLALLGKINSLRLSGLDL
                     TGELDPQGKITIAGWNDVQSPLPQILKLKNTLIEIENARLSVLSQKWGGTSLSLDMQA
                     RPQRQHVNLQGRITATQRTLSATASLEGQISNKGFWDLRMELEQGKVTLPNL"
BASE COUNT         1852 a         1726 c         1203 g         1425 t
ORIGIN      
        1 gttcgagtcc catcagtcgc cccactcttg caaatcattc tagtcttgta aaatccacct
       61 tccaaagtag aatgctttgc aagaaaggac ccccctgctc atgaccaccg ctctcaaatt
      121 caaaaatatt gccgaattcc tgaaatacat taaagaaaac gacatccagt atgttgacat
      181 gaatttcacc gatctgcgcg gaaaatggca acacacaacc gaatatgcac cggaactcga
      241 tgaatacaaa ctcaatcgtg gcattttctt tgacggctcc tccattgcag ggtggcaacc
      301 aattaacgac tccgacatgg tcatcaaacc ggacctcggt aaagtcgcca ttgacccttt
      361 cgccgcacaa agaacactca aggtcttttg cgacatccat gaaccaaact caggcaagcc
      421 ttatgcgctt gacccgcgct ccatcgccaa agccgcggaa aagcatctca aaagcagcgg
      481 cctggctgat acggcctatt tcggaccaga agccgaattt ttcatcttcg atgacgtcaa
      541 aatccatatg gaaaccaacc gcgtcagttt tgaaatcaac agcgaagaag ccccttacaa
      601 cgcagggcgc cattacgaca ccggcaatat gggccaccgc ccgcaaacta aaggcggtta
      661 tttccctgaa agcccggttg atcatctctc tgacattcgc ggcgaaatgc tgaccgttat
      721 ccagagcatg ggtgtcgaag tggaaaaaca ccaccatgaa gtcgccccca gccagtgcga
      781 actcggcatc aggttctcga ccctgctgga cagcgccgac aacatccagc tttacaaaca
      841 tgcggtacac aacgtcgcca acgcctacgg caaaaccgcc actttcatgc ccaaacccgt
      901 cttcggagat aacggctctg gtatgcacgt ccatcaatct ttatggaaat caggtaaacc
      961 tctctttgcc ggtgacagct acgccaacct gtctgaaaac gcgctctatt atatcggcgg
     1021 cattatcaaa catgcccgca gcctaaacgc ctttaccaac ccgacaacaa atagctataa
     1081 acgactcgtt ccaggttatg aagcgcctgt attgctggcc tattccgctc gaaatcgaag
     1141 tgcttcttgc cgcgttccat acagcgcttc accaaaaggc aagcgcatcg aagttcgttt
     1201 ccctgaccca accgccaacc catatctggc tttcgctgca atgttaatgg ccggacttga
     1261 cggaatagaa aacaaaattc accctggcga agccatggac gaaaatctct atgatttgtc
     1321 acaagaagaa caagataaac tgccgaatgt ctgccattct ttacgcagcg cactagaggc
     1381 tctacgtgaa gatcatgaat ttctgctaaa aggcgatgtc tttacgcaaa gtgcaatcaa
     1441 cgcctatatc ggcttaaaag aagaagaaaa caaagcgtat gaaatgatga tccaccctat
     1501 cgaatacaaa ctatactatt cctcataata ccaacatttt tctattccgc agttctgggt
     1561 tttatctcat ttacaaccag cttatatgtt tcaacattac gcgcccattt aaaataatca
     1621 tcgtcacgtg aaggcgtaaa cagggaaaca aaataataaa aatcgcctgc cgacataatt
     1681 gtcaaccaca gctcatgttc aataaccctt tcattttcat ccgtaacctt aaaaacctgt
     1741 gtatcaacaa aatcatagct atcaccaaga aaaaattcat ttcgattttc aataacatcg
     1801 cccataacaa gccccttttc cgcaacatcc tccttataat aattgagttc gtcatcaaga
     1861 gtctctgaag cataaataga cacaagatca ataccaatct gcccatccaa acgaatcttc
     1921 ccagtgtgag aaccatcatg tatgtaatgt gtgttaagca ataaaacttc catacgatca
     1981 atcgtacgaa taggtaacgc tttcaactcc caactgaccg gatacataaa ttcagccaaa
     2041 tccagaaact ggtaacgctg catttcttcg atatattcct taaccggagt aataggatca
     2101 aaggaatcaa gtaccatttt ctgcatgact ttctcatcat gccaacgctc gataggaaca
     2161 acatactgag catacataac gtgcttaccg tttgtataaa cactggcacg aacagcataa
     2221 ctaaccttgt ttttgataaa aacataaaga gcctccgcgc gtgtatcgtc atggacagta
     2281 agcccctgca cgttatagct gctggaaaga aaaaactgca taagccactg ctcaacagtc
     2341 atataataat ccatactcat cgcttcaact gtcaaataac tacgctcgga atatatgtct
     2401 ggcggcccgt aaaaccgtac aatttctccc ataattttat tggtaaccga aaaatcttcc
     2461 ggcctgccct ttgtcatatt ttcccatccc ttgggaattc gaatttcaaa attcaacgca
     2521 ggatcaccaa acgtttttag aaccaaaggt tccgtatttt ctgtaaactc accggaggaa
     2581 agtagatcaa attcgggcat aggtttacga tattgttcca taactttttg agcaaaagcg
     2641 ctatgaggaa aaaaccaaga aataccggaa accaacacaa gaagcacaca aatcctatgt
     2701 aaacttatca tttctaaaac acctcttatg ctttctttca gggcaataaa tggaacgcaa
     2761 acattattgt aacacatctc ttggccaagt ttccaaaaac acggtcttaa ttcataccag
     2821 gcttaaaaat ccgtgtattc ccctctacac ataggctaaa gcccttgatt tgcaggccat
     2881 agaactttac acaaaaacaa agtacgattt tttttaacgc tcacgtacaa agtaacagag
     2941 tttaataata tgtctgattt attcgctcca ccttctgctg catcaaaaaa caactacgat
     3001 gcctcttcaa ttgaagtttt ggaaggactt gagcctgttc gcaaacgccc cggcatgtat
     3061 attggcggca cagatgagcg cgccttgcac cacttggccg gcgaaattct ggacaactca
     3121 atggatgaag ccgtagcggg ccacgccgac tggatcgaaa tcgcccttga ggctgattac
     3181 tctcttatca tccgtgacaa tggccgcggc atcccggttg acgaacaccc gaaatacccc
     3241 ggaaaatcag ctcttgaggt catcatgacc acactacact caggcggaaa attctcagga
     3301 aaatcatacg aaacctcagg cggtctgcac ggtgtaggaa tttccgtcgt caacgcccta
     3361 tccgataaca tgtgggtcga agtcgcccgc gaaaagaaac tctaccgcca aagcttttcc
     3421 cgcggcctcc caacttcaaa actcgaagat ttaggcgcca ttaacaaccg ccgtggcaca
     3481 accgtatctt ttcaccccga tcctgacatt ttcggctcac aagcccgctt caaacccggc
     3541 tggctgtttc agatggcccg ctccaaagca tatctatatt ccggcgttga aattcgctgg
     3601 aaatgcgccc acgaaattat ccctgaagac agcaaaatca cgcctgaagc cgtatttaaa
     3661 ttccccaacg gactgcttga ctaccttgac gccacgctac ataaccgcaa aacagtaatc
     3721 cccaacccct tcgcaggcaa agccgatctc cccggtaatg aaggtcgtgt tgaattcgcc
     3781 atcacatggc ctgaaaacgg tgaaggcttt agccattcct actgcaacac cgtaccaaca
     3841 ccacaaggcg gcacccatga aaccgggctg cgcaccgcac tctcacgaag cctgcgcgcc
     3901 tatggcgaaa tgatcggcgt aaagaaggca aatatcatca ctgctgacga tgtgctgggc
     3961 ggcgcagcta tattgctttc tgtctttatc aaagaccccc agtttcaagg ccaaaccaaa
     4021 gaaaaactcg ccagttcagc tgtcagcaaa tgggttgata gcgccataaa agaccatttc
     4081 gacctttggc tttccggtga cccggcttca gccaccaagc tactaaacgc catcatcctg
     4141 cgtgccgaag aacgcctgcg tcgtaaaatg gaccgcgtac aggcccgcaa aagcgccaca
     4201 caaaagctgc gtctgcctgg caaactcgcc gattgttcac gcgccaatgc cgaagacacc
     4261 gagcttttca tcgttgaggg tgatagcgca ggcggctcag ccaaacaagg ccgcaaccgc
     4321 gaaacacagg ccatcttacc cttgcgcggc aaaattctca acgtcgtcag cgccaccgcc
     4381 gataaaatcc gcgccaatca ggaaattcaa gatctcatcc aggctctggg ctgcggcgct
     4441 ggccgagact ttcacctctc caaactccgc tatgaacgcg tcatcatcat gaccgatgca
     4501 gacgttgacg gcgcccatat cgccgcgctg ctcatcactt ttttttatac tcaaatgcgc
     4561 gaacttgtcg aagaagggca cctctatctc gcccaacccc cgctctaccg cctcgtcgca
     4621 ggcaataaca gcgcctatgc aaaagacgat gaacacaaag atgcactgat ggaaaccaag
     4681 gaattcaaag gaaaaaaagt tgaagtcagc cgttttaaag gtcttggaga aatgcccgca
     4741 aaacagctca aagaaacgac catgaaccca gccagccgca ccttgctacg cgtaaccctg
     4801 cccgaagccg ccgccgcact cgctatagga ctgtccggtg aagaactgga aagtctggaa
     4861 ggcgaaaacc cgcttgaagc cggcgcttca caggctgaca tcgatgatct cgtcgaacgc
     4921 ctgatgggca aaaacgccga gccccgcttt caattcattc aagacaacgc cgaattcgcc
     4981 agcgatattg acgtttaggt aaaaacattc gcaccgcacc agggataaaa aacttgaatt
     5041 ggataaaacg ccaactctat cgcacaattg actacggcca cgaagctaaa agacgtgctg
     5101 aacgaaggaa aagtctccag aattttttat taattccgtc tgttattttg tccacttctc
     5161 taatctggct cttaagcctc tattgttttt cacaatggca cgcatacatc tttccagaag
     5221 aaaccctcgc caatgcggaa ggaatagggc caattctggt aaccgtctca cctctttttt
     5281 tcgctctttt gtttgggatg attctaggaa acaagcttgt cgcattattt ccaacaacca
     5341 aacgagtcct tgagcaagaa gctcaaaaat tttcccaaac ctcttacaag gaatcacaaa
     5401 aacacctgct gagactatcg gtaattatca tccctttatc gttcggatta gccatttggg
     5461 gcgtttttct tccttggtag gctttttagc tcttaccgca ggaatatgtg cgctgagttt
     5521 aaacccctaa cattgacgtt taaccaacat acagcgatga tgaggcgtca tgaaacgccc
     5581 gattttaatt ctcgcactcc taatcatgac cggagccatc ggcgtcaccg gttatgtatg
     5641 gctgaacatc cctcaacacc ttgagaccag actccgacaa accatgaatg aagcaggttt
     5701 tgaaaattat accctcccac cggccgaaaa gaaacttgga caaaccgtct atcaaaacat
     5761 cgctcttgac cctgatggtt tcagcacact cgaaaacctg acaataacct acaatcccct
     5821 gaaacttgct ctgcttggaa aaattaactc tctgcgcctg tccggcctcg atttaaccgg
     5881 agaactagac ccgcaaggca aaatcaccat agccggatgg aacgacgtac aatcccccct
     5941 tccacaaata cttaaactta aaaacacgct catagaaatt gaaaatgcac gcctgtcagt
     6001 gctcagtcaa aaatggggcg gcacatctct aagtctggac atgcaagcgc gcccacaaag
     6061 acagcacgtc aatctacaag gcagaatcac agctacgcag cgtacgctca gcgccacagc
     6121 cagtctggaa ggccaaatca gcaacaaagg cttctgggat ctacgcatgg aacttgagca
     6181 aggcaaagtc actctgccca atttgc
//
LOCUS       DAOSWR010000121         6111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8492_length_6111_cov_4.925363, whole genome shotgun sequence.
ACCESSION   DAOSWR010000121 DAOSWR010000000
VERSION     DAOSWR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8492_length_6111_cov_4.925363"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..925)
                     /locus_tag="PK513_08545"
     CDS             complement(275..925)
                     /locus_tag="PK513_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO82537.1"
                     /translation="MSEGEINNIYEALPQGQVESLVILLHGLGSNGQDLISLAPYFAA
                     KVPDAAFVSPDAPFVCDMVPPGYPNSYQWFSFQDRDPDKMLTEIETAAPILQKFIADQ
                     IEKYQIPANKTALVGFSQGTMMSLYAAPRYPKTLAGVLGYSGSMIGEAGPNKIPVRLI
                     HGQADDVVPVEAYAHARSMLERSGFEVSGYTTPGLSHSIDETGIKSGTEFLKSVLL"
     gene            931..1986
                     /locus_tag="PK513_08550"
     CDS             931..1986
                     /locus_tag="PK513_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HOO82538.1"
                     /translation="MASMIMRTTQSLFLFGLFFLAGNVACAQEPSVEQQIVERVKNSE
                     NKNFVSLSVENDSIGGGTDQFYTSGVRLSWFNVNTPVPPMIDEMADMVPTFDLNETTS
                     TLFTLGQNIYTPEDIRIAANQDNDRPWAAFLYGSVGLATLENNHIDELEVTLGIVGPE
                     ALGEQAQKFIHSHVTSSPTPKGWRHQLDFEPGLIISAQRRWPGAYHKTIGDYRLRAEP
                     NVNASLGNIYTYIGSGVSLSFGPYQGYLQDTPPRVRPAMPGSGYFETPDQNWSWYLFA
                     GLDGRLMGHNIFLDGNTFKSSHSVDKKPFVGDVSAGVAFTLGNYRLSYAINGRSKEFD
                     GQKGTSVFGSLTLSTRF"
     gene            2129..2443
                     /locus_tag="PK513_08555"
     CDS             2129..2443
                     /locus_tag="PK513_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO82539.1"
                     /translation="MKYDLLHIQGQPYVLVPLHDYRSMVSGGKNKGLPDEILDALTAR
                     EESPVKIMRKHRGMTQKELAALAGISRPYLTEIETGKKDGSIRAMKALADVLGVSVGA
                     LA"
     gene            2483..2965
                     /locus_tag="PK513_08560"
     CDS             2483..2965
                     /locus_tag="PK513_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO82540.1"
                     /translation="MFTYRLTQPWEYDEIAALNWLPQSVRDAFSRFSDRTKDIIAFKY
                     INNGIIEGVGIAKFKHNRLGTHIVIGHLNVKDGCRGQGIGSALLNHFLEISVQNHVPC
                     RLQVAPFNRGAKRLYGRFDFSVAPDEQDPGTLWMARYPLSFQPAHNAAHDFAGDSAAY
                     "
     gene            complement(2903..4390)
                     /locus_tag="PK513_08565"
     CDS             complement(2903..4390)
                     /locus_tag="PK513_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOO82541.1"
                     /translation="MKKLRDDAVYVGQSVGKEKLAQYLPLKWGNRHGLIAGATGTGKT
                     VTLQNLAEGFSDVGVPVFMADVKGDLSGICQPSEMQDFLTKRADIIGFGDEYKARAYP
                     AVFWDLFGDQGHPIRTTVSEMGPLLLARILELNDTQEGVLNIAFRVADEQGLLLLDLK
                     DLRALLNTVHEQRAEISKTYGNVSPQSVGAIQRSLLRLEDQGAADFFGEPALDLRDFM
                     RTDLDGRGHVNILAADKLMQSPRLYATFLLWLLAELFEELPEAGDADRPKLVFFFDEA
                     HLLFDDAPKALLDKVEQVVRLIRSKGVGVFFITQNPADVPDSILGQLGNRIQHALRAF
                     TPKQQKAIKLAAQTYRENDKFDVAEVITELGVGEALVSTLEGNGQPSIVQRTLIRPPS
                     SRLGPASPDAKTMQMKASGLGTKYNQTQDRESAYEVLQLRAKKAAEQAEKAQEAAENA
                     QPKHRSSRQSVGEAAVKSMVRSISSSIGRTIAREVMRGIMGGLKR"
     gene            complement(4552..5718)
                     /gene="metC"
                     /locus_tag="PK513_08570"
     CDS             complement(4552..5718)
                     /gene="metC"
                     /locus_tag="PK513_08570"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HOO82542.1"
                     /translation="MSDKKFKEDTLLTKLGRDPDDYHGIMNPPIVQASTIKYKDLEGY
                     ETKKGQKFTYANISHPLSNHFETAMAELEGGYKAVSSSTGLAPITAALLAFLKAGDHL
                     LTCDSIYPPTRIACDKLLGRFGVEVEYYDPCVGAGIKDLIKDNTAVIYLESPGSATFE
                     VQDVPAIVKEAKARGVTTIMDNTWASGLLFKPLAHGVNISVCAATKYISGHADVNLGY
                     AVADTEEHYKRLKSAHVDLGHCPGSLDLSLALRGLRTMKMRMKRQAASALQIAQWLQG
                     RDEVRRVYHVALPDHPTHALWKRDFSGSNGLLSILLKDYSKEQVSNFINVLELFPIGS
                     SWGGYMSLLQPQIEIGWRKAKKWNETGQLLRLQIGLEDPDDLIADLEQAFKKLK"
     gene            complement(5715..>6111)
                     /locus_tag="PK513_08575"
     CDS             complement(5715..>6111)
                     /locus_tag="PK513_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOO82543.1"
                     /translation="ANTPWGRIGLAICYDLRFPHLWRDMAKAGAQILTAPSAFTMPTG
                     QAHWEVLLRARAIETGSFVLAAAQTGEHEGGRQTWGHSMIISPWGEILSAMEREIGII
                     TAEIDLNEVEKARASIPALQHDRDYEIKI"
BASE COUNT         1405 a         1550 c         1617 g         1539 t
ORIGIN      
        1 taacatagtg cggttcatag tgctgatcag acctcgcgca tctaacactt tcattatacc
       61 ataaacccgc gcctttccta agtttttcgg tggtttctat gctgcacaac accaatttcc
      121 gtattgcaat atttttcata taccataata attttatgat tattcagtgg gttatacctc
      181 cttgcgaaag caaaaaagag gaactgtgca gtgcgatatt caagttgaat taatttataa
      241 aaaaatacgc cgcaacgctt tgaaaaaaca tttcttacaa taatacagat ttcaaaaatt
      301 ctgtgcctga ttttatccct gtttcatcga tagaatgcga taggcccggc gtggtgtaac
      361 cactcacttc aaagccgctg cgttctaaca tgctgcgtgc atgagcgtat gcctcgacag
      421 gaacaacatc gtcagcctgc ccatgaataa ggcgcaccgg gattttattt ggtcccgctt
      481 ccccgatcat agaaccggaa tagcccagta caccggccag cgtttttggg tatctgggtg
      541 cggcatacag actcatcatc gtgccctgag aaaagccaac cagcgcggtc ttatttgcgg
      601 ggatctgata tttttcaatt tgatcagcga taaatttctg caatatgggg gcggcggttt
      661 cgatttctgt gagcatttta tcgggatcac gatcttgaaa ggaaaaccat tgatagctgt
      721 ttggatagcc tggggggacc atatcgcaca caaacggcgc atcgggtgaa acgaacgcgg
      781 cgtcaggcac tttcgccgca aaatacggcg ccaatgagat aagatcctgc ccgttcgagc
      841 ccagcccgtg cagcaaaatc accagacttt cgacctgccc ttgcggcaat gcttcataaa
      901 tattgtttat ttcaccttca ctcatgctat atggctagca tgatcatgcg cacgacccaa
      961 agcctattcc tattcggcct gttttttctt gcgggcaatg ttgcctgtgc gcaagagcct
     1021 tcggttgagc agcagatcgt cgagcgcgtt aaaaactctg aaaataaaaa ttttgtcagc
     1081 ctgtcggtag aaaatgactc tattggcggg gggacggatc agttttacac cagcggcgtg
     1141 cgcctgagct ggttcaatgt gaatacacct gtgccgccta tgattgatga aatggcggat
     1201 atggtgccga cttttgatct caacgaaacc accagcaccc tttttacatt ggggcagaac
     1261 atctatacac cggaagatat tcgtattgcc gccaatcagg ataatgaccg gccatgggcg
     1321 gcgtttcttt acggttctgt ggggctggcg acactggaaa ataaccatat tgatgaactg
     1381 gaagtaacgc tcggcattgt tggcccggaa gctttgggtg aacaggcgca gaaatttatt
     1441 catagccatg tgacaagttc tccaactcct aaaggctggc ggcaccagct tgattttgag
     1501 cctggactta tcatctccgc gcagcggcgc tggccgggag cttatcacaa gaccatcggc
     1561 gattaccgtt tgcgcgctga gccgaatgta aacgcctctt taggaaatat ttatacctat
     1621 atcgggagcg gtgtcagcct ctctttcggc ccgtatcagg gatacttgca agatacgccg
     1681 ccgcgggtgc gtcctgccat gcccggatca ggttatttcg aaacgccgga tcagaactgg
     1741 agctggtatc tgtttgccgg gctcgacggg cggcttatgg ggcacaatat ttttctggat
     1801 ggcaatacat tcaaaagcag ccacagcgtc gataaaaagc cttttgtcgg cgatgtctcc
     1861 gcaggggtcg cctttacgct tggcaattat cggctctcct atgcgattaa tggacgctcg
     1921 aaagaatttg acgggcaaaa gggaacaagc gtttttggat ctttaacgct gtcgacacga
     1981 ttctagctcc ggcgtcattg cgagcgcacg tgaagcaatc catctattgg gcagaactca
     2041 gagatggatt tccgcgtcag accttctgcc ctcctcgcaa tgacgaaatg cagtgacaat
     2101 gtcgctttca gcccgtatat tctaatttat gaaatacgat cttcttcata ttcagggaca
     2161 accctatgta ctggtgccgc tgcatgatta ccgcagcatg gtcagcggcg ggaaaaataa
     2221 gggactgccg gatgagattc tcgatgcgct cacagcccgc gaagaaagcc cggtgaaaat
     2281 tatgcgtaaa catcgcggca tgacgcaaaa ggaactggcg gcgctggccg gaatttcacg
     2341 gccctatctc accgaaattg aaaccggaaa aaaagacggc tcgattcgcg cgatgaaagc
     2401 gctggccgat gtgctgggcg tgagcgtcgg ggcgctggct tagtttgaca agtttatact
     2461 caatatatta tacataatca ttatgtttac ataccgactt acacaaccct gggaatatga
     2521 tgagattgcg gccttaaact ggctgccgca gagcgttcgt gatgcgtttt cccgtttcag
     2581 cgaccggacg aaagatatca tcgcctttaa atacatcaat aatggaatca ttgaaggtgt
     2641 tggcattgcg aaatttaaac ataatcgtct gggaacgcat atcgtcattg gccacctgaa
     2701 tgtcaaggat ggctgtcgag gccaaggcat cggcagcgct ctgctcaatc attttttaga
     2761 gatttctgtt caaaatcatg ttccttgccg actgcaagtc gctcccttta atcgcggggc
     2821 caaacggcta tatgggcggt ttgatttttc tgtggctccc gatgaacagg accctggaac
     2881 actttggatg gccagatatc cgttatcgtt tcaacccgcc cataatgccg cgcatgactt
     2941 cgcgggcgat agtgcggcct attgaagagc tgattgaacg caccatagat tttacagccg
     3001 cttcgccgac gctttgacgg ctggagcggt gtttgggttg agcattctca gccgcctctt
     3061 gcgctttttc agcctgctcg gcggcttttt tggcgcgcag ctgcaatact tcataggcgc
     3121 tttcgcggtc ctgtgtctgg ttatattttg tgcccagacc gctggctttc atctgcatgg
     3181 ttttggcgtc aggactagcc gggccgaggc gtgaggatgg tgggcggatt agtgtgcgct
     3241 ggacaattga gggctggcca ttaccttcca gagtcgagac cagcgcctcg ccgacaccca
     3301 gttcggtgat gacttcagcc acgtcgaatt tgtcattttc gcggtaagtc tgggctgcca
     3361 gcttgatggc cttttgctgt ttgggggtga aggcgcgcaa ggcgtgctgg atgcggtttc
     3421 cgagctgacc caagatactg tcaggaacgt ctgcggggtt ttgagtgata aagaacacgc
     3481 caacgccttt ggaacgaatc agacgcacga cttgctccac cttatcgagc agcgccttgg
     3541 gcgcgtcatc aaaaagcaaa tgcgcttcat cgaaaaagaa gaccagtttc gggcggtcgg
     3601 cgtcaccagc ctctggcagt tcctcgaata attcagccag cagccagagc aggaaggtgg
     3661 cgtagagccg gggggattgc atcaatttat cggcggcgag gatgttgaca tggccacgcc
     3721 cgtcgagatc ggtgcgcata aaatcgcgta agtccagcgc gggttcgccg aagaaatcag
     3781 ccgcgccttg atcttcgagg cgcagaagtg aacgctgaat tgcgccgacg ctttgcgggc
     3841 tgacattgcc gtaggttttg gaaatttcgg cgcgttgttc atgcacggta ttcaggagtg
     3901 cacgcagatc tttaaggtcg agcagcagca ggccttgttc gtcggccacg cggaaggcga
     3961 tattgaggac gccttcctgt gtgtcgttaa gttcgagaat gcgcgccagc aaaagcgggc
     4021 ccatttcgga aacagtcgtg cggatcggat ggccctgatc cccaaacaaa tcccagaata
     4081 cggctgggta ggcccgcgct ttatattcat cgccaaaacc gatgatgtca gcgcgtttgg
     4141 tcaggaaatc ctgcatttct gagggctggc aaataccgga caggtcgccc ttcacatcgg
     4201 ccataaagac cggcacaccc acatcagaaa agccttcggc caaattttgc agcgtgacag
     4261 tttttcccgt tccagtcgcc ccggcgatca gaccatgacg gttcccccat ttcagcggga
     4321 ggtactgggc gagtttttct ttgccaacgc tttggccgac ataaacggca tcgtctctaa
     4381 gctttttcat gcttcattcc tctttgtcat tgcgagcgcg aagcaaatgc atagcatttg
     4441 ttcgcatttt tataaagcaa acaaatctgt gagttgcttt gcgcgaagca atccagaaac
     4501 agcaaaacaa aaatggattg ccgcgtcgtc tttgcctcct cgcaatgacg gttactttag
     4561 tttcttaaat gcctgttcca ggtcagcgat gagatcgtct ggatcttcaa ggccgatttg
     4621 cagacgcaag agttgtccgg tttcgttcca ttttttggct ttgcgccagc cgatttcgat
     4681 ttgcggctgg agcaggctca tatagccgcc ccatgaactg ccgatgggaa agagttccag
     4741 cacgttgatg aaattactga cctgctcttt ggaataatct ttgagcaaga tgctgagcaa
     4801 tccgttagaa ccggaaaaat cacgtttcca cagagcgtgg gttgggtgat cgggcaacgc
     4861 gacgtgataa acgcggcgca cctcatccct gccctgtagc cactgcgcga tttgcagggc
     4921 gctggcggcc tggcgcttca tacgcatttt catggtgcgc aacccgcgca gagccagcga
     4981 taaatccaac gagccggggc aatggccgag atcaacatgc gcggatttga ggcgcttata
     5041 atgctcttcg gtatcggcga cggcgtagcc caaattcaca tcggcatggc cgctaatata
     5101 tttggtcgcg gcgcagaccg agatattgac tccatgcgcc aaaggcttga acagcagacc
     5161 gctggcccaa gtgttatcca tgatcgtcgt aacgccgcgt gctttggctt ctttgacaat
     5221 ggcggggaca tcctgcacct cgaatgtcgc cgagccgggt gattccagat aaattacggc
     5281 ggtgttatcc ttgatcaggt ctttgatccc tgcccctacg catggatcat aatattcgac
     5341 ttcgacgccg aaacgtccca gaagtttatc gcaggcgatg cgggtgggcg gatagatgct
     5401 gtcgcacgtc agcagatgat cgcctgcttt taaaaaagcc agaagcgcgg cggtaatcgg
     5461 ggcgagaccg gtcgaagagc ttaccgcctt atacccccct tccaactccg ccattgccgt
     5521 ttcgaaatga ttgcttaagg gatggctgat attggcgtag gtaaatttct ggcctttttt
     5581 ggtttcgtag ccttccaggt ccttatattt aatcgtggag gcctgcacga tcggcgggtt
     5641 cattatgccg tgataatcat ccgggtcgcg gcccagtttg gttagaagcg tgtcttcttt
     5701 gaatttcttg tcgctcatat ttttatctca tagtcccggt catgctgaag cgcggggata
     5761 ctggcgcgcg ctttttcaac ttcatttaaa tcgatttcag ccgttatgat accgatttcc
     5821 cgctccattg cagacaaaat ctcgccccag gggctgataa tcatcgaatg cccccatgtc
     5881 tggcgtcctc cttcatgctc gccggtctgg gcagcggcca aaacgaagct gccggtttcg
     5941 atggcgcggg cgcgcagcag gacttcccaa tgcgcctgac cggtcggcat ggtgaaggcg
     6001 ctcggcgcag tgaggatttg cgcgcctgct ttggccatat cgcgccagag atggggaaag
     6061 cgcagatcat agcaaatggc caggccgatg cgcccccagg gggtattcgc g
//
LOCUS       DAOSWR010000122         6028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_8655_length_6028_cov_4.727942, whole genome shotgun sequence.
ACCESSION   DAOSWR010000122 DAOSWR010000000
VERSION     DAOSWR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8655_length_6028_cov_4.727942"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK513_08580"
     CDS             complement(<1..285)
                     /locus_tag="PK513_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOO82544.1"
                     /translation="MFLVIDNYDSFVHNLARYFEIAGVKTKIVRNDAITLKEIKALKP
                     EALILSPGPCTPKEAGICIEAVKTLGPTLPILGVCLGHQAIGEAYGALTLR"
     gene            411..1052
                     /locus_tag="PK513_08585"
     CDS             411..1052
                     /locus_tag="PK513_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82545.1"
                     /translation="MPKIVAIIKAVDEDRAVARILTRFNAVTAGTRWSDLDRFQCLRH
                     ALEDEADSLRSIGDRGRSVVSEALNKAVSGLSGQYNGDARHDVSKSVVWCGLLEDSDV
                     QRIVDRFASVSEGETDVVKTHRFVKVIMQEAEALERLNGKKEERMRVAIALQSAFEQV
                     PEGGGYRDQHALFAIEERKPVWKEYLSFCRAGVDQGVLTNEVQNNAEVELTHS"
     gene            complement(1038..2453)
                     /gene="pabB"
                     /locus_tag="PK513_08590"
     CDS             complement(1038..2453)
                     /gene="pabB"
                     /locus_tag="PK513_08590"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOO82546.1"
                     /translation="MTPFIRELPIRDPLEAFSALEHLPYSLLFDSADRDHPNARYSFV
                     VSHPIETLEYKDGSMQITNWEEQLNIEGEPFKIIKERIKKWVPESQPVRGLPPFQGGL
                     AGYFGYDVGRALEKLPQDAADNPDMPDLALGIYDQVLAFDHKKNQAWIITHAPSYHEA
                     RRKQDYVLGLIKHPTHHAPHQNTHLEWESNFDACDYMNMVDKVIEYIKAGDIFQANVS
                     QRFDANLPEGFSPFAHYRHLREVNPAPFAAYMNCGDIKISSASPERFLTVKDSRVETR
                     PIKGTRPHIANTALDQKYRYELENSDKDRAENTMIVDLLRNDLSKVCKVKSIKVSELC
                     KLETFASVHHLVSTIHGELKSGKTPLDLMKACFPGGSITGAPKIRAMEIIEELEPSRR
                     GPYCGAMGYIGFDGAMDSNILIRTLVYEDRKVSFQVGGGIVADSKPEDEYQETFYKAE
                     GLFRSFEPPRHETEEEILRVG"
     gene            complement(2713..5622)
                     /locus_tag="PK513_08595"
     CDS             complement(2713..5622)
                     /locus_tag="PK513_08595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOO82547.1"
                     /translation="MLIQTAPAPTTAITLPDIPALYVNARSAYMLTADGELKTLSHDQ
                     AAALIHKKPALVCHAPFCRAKLGNDVQFAAFDVLELFAFVHPATFSTPTVNGLCKTLG
                     LAEPTSLDDAPMSLLDITRALLGDLRADPHKAKADPLEIARVMGLNGKGWAWTPFVFS
                     ALGQPYDEDVPVNSKTGLNIWKNLPEWAEEAPPPPASHHGVTEDEARERLAKLLGPEA
                     EKREEQMQYASMIAKAFTPPISSQHNNPLPLGGEGQGGEASLHPPPIPLPQGRGDSSF
                     ITPTSIIHYSHIILAEAGTGVGKTLGYLAPASVWAEKNQGTVWISTYTKNLQRQIDQE
                     LDRLYPNPELKTVHVATRKGRENYLCLLNLEEAAAGAALAKHPDAVIAAGIMARWTAA
                     SKDGDLTGADYPGWLAGLLGYRHTSALADRRGECIYSACDHYHKCFVERSVRSAAHAR
                     IVVANHALVMISAALSTPGEDMPTRYIFDEGHHLFEAADSAFAAHLTAREARDLRRWI
                     LGNESGRRASRARGLKRRAEDLTEGMQDAEELLRKILNVAECLPMDGWTRRLRDGLPS
                     GPTEQFLAQIYKQIHARANGRDGPYSLETELLPVDPDVLTASHNLKTALQHLQKPMQS
                     LARIFKKKLANDEGLLDTDTRKRLEALSSSLERRATLTLQAWIQMLENLDKGPSSDST
                     SEFIDWMEIQRIDGHAVDVGLYRHHIDPMQPFATSIRPHIHGMAVTSATLRDSSQEDQ
                     ADWTSALKRTGAAYLNPNTETHSFASPFDYANQTKIFVINNVNKHDLDQVAGAYRALF
                     KAAGGGALGLFTAISRLRAVQSHIVDELEQSGLPLYAQHVDEIDTGTLVDMFRENEHA
                     CLLGTDAVRDGVDVPGNALRLIVFDRVPWPRPTILHKARRYAFGGRAYDEMITRLKLK
                     QAFGRLIRSEGDKGVFVMLDSGLPTRLQSAFPKNVEVVKTGLSEVITAIQKHLDT"
     gene            5759..>6028
                     /locus_tag="PK513_08600"
     CDS             5759..>6028
                     /locus_tag="PK513_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82548.1"
                     /translation="MDFWGFGKESGHDVIVRHPSLASRFADVGIPERTQTQNIYVSPQ
                     TQQSIDQLDPKLLGVVAEISGADNVIFISDDDIRPEIEKIDAQRYG"
BASE COUNT         1281 a         1319 c         1874 g         1554 t
ORIGIN      
        1 gcgcagggta agggcgccgt aggcttcacc gatggcctgg tgaccgagac atacgccgag
       61 gataggcagc gtggggccga gcgttttgac ggcttcaata catatgcctg cttctttcgg
      121 ggtgcatggg ccgggggaaa ggatcagcgc ttcgggtttt agggctttta tttctttaag
      181 cgtgatggcg tcgttgcgga cgattttagt tttgaccccg gcgatttcga aataccgcgc
      241 gaggttgtga acgaaggagt cgtaattgtc gatgaccaaa aacatggctc tttaaagcat
      301 attgaagggg ctgctggcaa tatatgaaaa ttctgtacca ttgtgcattt ttgagttgtt
      361 atttgctata aaaactgtat aagaattcct tcaaagcagg aggatattgt atgccgaaaa
      421 ttgtagcgat aataaaagct gttgacgaag atcgcgctgt tgctcggatt ttaacgcgtt
      481 ttaatgcggt tactgcagga acacgctgga gcgatcttga tcggtttcaa tgtcttcgcc
      541 atgccttaga ggatgaggcg gatagtttac gttcgattgg tgatcgtggt cggtctgttg
      601 tgtctgaagc gttgaacaaa gctgtttcag gactttccgg acaatataac ggggatgcac
      661 gtcatgacgt atcaaaaagc gttgtatggt gtggattgtt agaagattct gatgtgcagc
      721 gtattgttga taggttcgct tctgtatctg aaggggaaac agatgtagtt aaaacgcatc
      781 gttttgttaa agttattatg caagaggctg aggctttaga acggttgaat ggtaagaaag
      841 aagagcggat gcgtgttgct attgctttgc aaagcgcttt tgaacaagtt ccagagggtg
      901 gaggttacag agaccagcac gcactttttg ctattgagga aaggaagccg gtttggaaag
      961 aatatctttc tttttgcagg gcgggtgtcg atcaaggtgt acttacaaac gaagtgcaga
     1021 ataacgcgga agtggaatta acccactcgt aaaatttctt cttcggtttc gtggcgcggg
     1081 ggctcgaagc tgcggaagag gccttcggct ttatagaagg tttcctgata ttcgtcttcg
     1141 ggctttgaat cggcgacaat gccgccgccg acctggaagc tgactttccg gtcttcataa
     1201 accaatgtgc ggatgaggat gttgctgtcc atggcgccgt caaagccaat ataccccatg
     1261 gccccgcaat agggaccgcg ccgcgaaggt tccagttctt cgataatttc catggcgcgg
     1321 attttgggtg cgccggtgat ggagccaccg gggaagcagg ctttcatcag gtcaagcgga
     1381 gttttgccgc ttttgagttc accgtgtatg gtggagacca gatgatgcac gctggcgaag
     1441 gtctcgagct tgcataactc gctgaccttg atcgatttaa ctttacagac ttttgacaga
     1501 tcgttgcgca gtaaatcaac gatcatggtg ttttcggcgc ggtctttgtc gctattttcg
     1561 agctcgtagc ggtatttttg atccagtgcg gtgttggcga tatgggggcg ggtgcccttg
     1621 atgggacggg tttcaacgcg gctatccttg acggttagga aacgttctgg cgaggcggaa
     1681 gagattttta tgtccccaca gttcatatag gcggcgaacg gggccggatt tacttcgcgc
     1741 aaatggcggt aatgggcaaa ggggctgaaa ccttccggca ggtttgcgtc aaaacgctgg
     1801 gatacgttgg cttgaaaaat atcaccggcc tttatgtatt caataacctt atcaaccata
     1861 ttcatgtaat cgcaggcatc gaaattggat tcccattcga gatgggtgtt ctgatgcggc
     1921 gcgtggtggg ttgggtgttt gatcaggccc agtacgtagt cttgtttacg gcgggcttcg
     1981 tgataactgg gcgcgtgagt gataatccag gcctgatttt ttttatggtc gaaggccagt
     2041 acctgatcgt aaatgcccag cgcaaggtcg ggcatatcgg ggttgtcggc ggcgtcttgg
     2101 ggaagtttct ccagtgcgcg gccaacatcg tagccaaaat aaccagccag tccgccctga
     2161 aatggtggca agccacgaac gggctggctt tcaggaaccc atttttttat gcgctctttg
     2221 atgattttga agggctcgcc ttcgatattc agttgttctt cccaattggt gatttgcata
     2281 gagccgtctt tatattccag tgtttcaatc gggtgggaga cgacaaagga atagcgggca
     2341 ttgggatggt cacgatcagc gctgtcgaac agcagggagt atggtaaatg ttcaagggcg
     2401 ctgaacgcct ctagaggatc gcggattggc agttcccgga taaaaggcgt cattatgctg
     2461 gctcccttca cgcaaacatt ccctgttgtt gtattttttg ctattgcgag cagtttagac
     2521 tgcatttttg ttcacagcaa catttttagt tttgggcgat tctgtctata agagaattct
     2581 aaaatgaatt ttatccacat tttcgagatt ttcgtcattg cgagcgcaag cgaagcaatc
     2641 catctcttgt aagagctttt tgtgatagat tgccgcgtcg ctgacgctct tcgcaatgac
     2701 ggcgtgaggt tcttatgtgt ctaaatgttt ctgaatggcg gtgatgactt cggaaaggcc
     2761 tgtttttact acctctacat tcttgggaaa ggcgctttgc aggcgggtgg gcaggccgga
     2821 atcgagcatg acaaacacgc ctttatcgcc ctcgctgcgg atgaggcggc cgaaggcttg
     2881 tttgagtttg aggcgggtga tcatttcgtc ataggcgcgc ccgccgaacg catagcggcg
     2941 ggctttgtgc aatatagtcg ggcgtggcca gggaacgcgg tcaaaaacaa tcaggcgcaa
     3001 ggcattgccc ggaacatcta ccccgtcgcg cacggcatcg gtgccaagca ggcaggcatg
     3061 ttcattttca cgaaacatat cgaccagcgt gccggtgtcg atttcatcaa catgttgggc
     3121 gtagagcggc aggccggatt gttccaattc atcgacgatg tgggactgga cagcgcgcag
     3181 gcgcgatatg gccgtgaaaa gaccgagtgc tccgccgcct gcggccttga acagagcgcg
     3241 ataggccccg gcgacctgat cgagatcgtg tttattgaca ttattgataa cgaaaatttt
     3301 ggtctggttt gcgtaatcaa atggtgaggc aaagctgtgg gtttctgtgt tgggattgag
     3361 ataggccgcg cccgtgcgtt tcaaggcact ggtccaatcg gcttgatctt cctgtgagga
     3421 atcgcgcaag gtggctgagg ttactgccat accgtggatg tgaggccgga tgctggtggc
     3481 gaagggctgc atcgggtcga tatggtggcg atacaacccg acatcgacgg cgtgtccgtc
     3541 aatgcgctgg atttccatcc agtcgataaa ttctgaagta ctatcagagg agggtccttt
     3601 atcaaggttt tcgagcattt gtatccaggc ctggagcgtc agggtggcac ggcgctccag
     3661 agaagagctg agggcttcga ggcgtttgcg ggtgtcggta tcgaggaggc cttcatcatt
     3721 ggcgagtttc tttttgaaaa tgcgcgcaag gctttgcatc ggtttttgta aatgttgtag
     3781 tgcggttttg agattatggg aggctgtgag cacatcgggg tctaccggta aaagttcggt
     3841 ttctagtgaa tatggcccgt cacggccatt agcgcgggcg tggatttgct tataaatttg
     3901 cgcgagaaat tgctcggttg gaccggaggg gaggccgtcg cgcaggcgcc gggtccatcc
     3961 atccatcggc aggcattcgg cgacgtttaa gattttgcgc agaagctctt cggcgtcttg
     4021 catgccttct gttaagtctt cggcgcggcg tttgaggcca cgggcgcggc tggcgcggcg
     4081 gccgctttca ttgccgagaa tccagcggcg caggtcgcgg gcctcacgcg cggtgaggtg
     4141 ggcggcaaag gcgctgtcgg cggcttcgaa gagatggtgg ccctcatcga aaatatagcg
     4201 tgttggcatg tcttcgccgg gggtagagag tgcggcggag atcatgacca gtgcgtgatt
     4261 agcgacgacg atgcgggcgt gggcggcgct gcgcacggag cgttcgacaa agcatttgtg
     4321 gtaatgatcg caggcggagt agatgcattc gccgcggcga tcggccagag cggacgtgtg
     4381 gcggtatccc aagaggccag ccagccagcc gggatagtcc gcgccggtga ggtcgccgtc
     4441 tttgctggct gcggtccaac gcgccatgat tccggcggct ataaccgcat ctgggtgttt
     4501 ggcgagcgcc gctccggcgg ctgcctcttc gaggttcagc aagcagagat agttctcgcg
     4561 gcccttgcgg gtggccacat ggacggtttt tagctcaggg ttgggataga ggcggtcgag
     4621 ttcctgatcg atctggcgct ggaggttttt ggtgtaggtg gaaatccaca ccgttccttg
     4681 gttcttttcc gcccagacgc tcgccggggc aagatagcca agggttttac caacgccggt
     4741 acctgcttcg gcgaggatga tgtgggaata atggatgatg gatgttgggg taataaaaga
     4801 ggagtccccc ctcccttgag ggagggggat agggggaggg tgtaaagatg cctccccacc
     4861 ctgaccctcc cccccaaggg ggaggggatt attgtgttgg gaggagattg gcggtgtaaa
     4921 tgcctttgca atcatgctgg catattgcat ttgttcttcg cgtttttcgg cttcggggcc
     4981 gaggagtttg gcgaggcgct cgcgcgcttc gtcctcggtt acgccatggt gagaggcagg
     5041 tggaggaggg gcttcttcgg cccattcggg caggtttttc cagatgttta atccggtttt
     5101 tgaattgacg ggcacgtctt catcatatgg ctgtcccagc gcggaaaata cgaagggcgt
     5161 ccacgcccag cctttgccgt tcaggcccat gacgcgggcg atttcgagtg gatcggcctt
     5221 ggccttatgc gggtcggcgc gcagatcacc gagcagggcg cgggtaatgt ccagcaagct
     5281 cattggcgcg tcgtcgaggc tggttggttc ggctaggccc agtgttttgc acaggccgtt
     5341 tacagttggg gttgaaaagg tcgccgggtg gacaaaggcg aaaagttcga gcacgtcaaa
     5401 ggcggcgaat tgcacatcgt taccaagttt ggcacggcaa aagggtgcgt ggcaaactaa
     5461 tgccggtttt ttatggatga gggctgcggc ctgatcatgg gatagcgttt taagctcgcc
     5521 gtcggcggtg agcatataag cgctgcgcgc attcacgtac agcgccggaa tatcgggcag
     5581 ggtgatggcg gtggttggcg ctggcgcggt ttgaatcaac atgccttaga attagcattt
     5641 tcgaaggcca atagctgcaa aaagccgatt ttgcgcgcaa aaagcccctg ataaagcctt
     5701 ctttaacggt tttcctatag aattgtggtg agttcgagcg aaaaagaaaa gcggctatat
     5761 ggatttttgg ggctttggaa aagagagcgg gcacgatgtg attgtaagac accccagtct
     5821 tgcgagccgt tttgctgatg tcggaattcc tgaacgaact cagacccaaa atatatatgt
     5881 atctccgcaa acccaacaaa gtatcgatca gctcgatcca aaattgcttg gtgtggtcgc
     5941 agagatttcc ggcgcggata atgtgatttt tatttcggat gacgatattc gtccggaaat
     6001 tgagaaaatt gatgctcagc gttacggc
//
LOCUS       DAOSWR010000123         5793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_9176_length_5793_cov_5.506971, whole genome shotgun sequence.
ACCESSION   DAOSWR010000123 DAOSWR010000000
VERSION     DAOSWR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_5793_cov_5.506971"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /gene="nuoN"
                     /locus_tag="PK513_08605"
     CDS             complement(<1..1250)
                     /gene="nuoN"
                     /locus_tag="PK513_08605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOO82549.1"
                     /translation="MIDFNLIPVLPEIFLSLIGMGLLIVGASQGNVSTRVICWSAVCG
                     LALSALILLSVGWETRSVLGGMFVMDKFAGFMKLMIIIGLIASLALSVRYLLQEGMER
                     FEFPILVLFAGIGMMIMVSSNNFLALYMGLELQSLSLYVLAAFHRNSMRSSEAGIKYF
                     LLGALSSGMLLFGVSLIYGFTGSVNFGVVGATLSDLDGVPIGVTFGLVFVLAGLAFKV
                     SAVPFHMWTPDVYEGAPTAVTALFAIVPKIAAMGLLMRLLFEPFMALSEQWVQIIYFL
                     ALMSMFLGAFAGIAQDNIKRLLAYSSIGNMGYALIGVVAGSAAGAGAVVLYLFIYMIM
                     SAGVFAIVMCMRRRGMAVYKISDLAGLSRHAPGLAYGMAILMFSMSGIPPAAGFFGKL
                     AVFNAAVAEGYYILATLGVLTSVVA"
     gene            complement(1247..2785)
                     /locus_tag="PK513_08610"
     CDS             complement(1247..2785)
                     /locus_tag="PK513_08610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOO82550.1"
                     /translation="MVDFPLLSLMTFLPVAGVLIILMIRGDEGTVKQNARHMALYTSL
                     FTLAFALFMYTRFDGSSAEFQFVEQGGWLKPLGINYHVGVDGISVFFVLLSAFLTPVC
                     ILSAWDAVKVRVKEFMVAFLLLESFMIGTFIALDAVLFYVFFEGVLIPMFLIIGVWGG
                     PRRVYSAYKFFLYTLLGSVLMLIGLLALYAQTGSTDIPTLMDNPVATHLQYWLWLAFF
                     ASFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGVLLKMGGYGFLRFSLPMLPEASAYF
                     ADFVFALSVIAIIYTSLIALVQEDMKKLIAYSSVAHMGFVTIGIFSVTTQGIEGGIFQ
                     MLSHGLISGALFLCVGVVYDRMHTREIERYGGIVKNMPKYATVFMILMLGSVGLPGTS
                     GFVGEFLVLLGAFQVSTLVAALAATGVIIGAAYMLILYRRVVFGPRVNDDALSMPDLN
                     WRELGLLVPIVLLVLWLGVFPGVVTEKIGPSVDKLITEYNAKLNAAAVVETPETGMLQ
                     EVGE"
     gene            complement(2789..4843)
                     /gene="nuoL"
                     /locus_tag="PK513_08615"
     CDS             complement(2789..4843)
                     /gene="nuoL"
                     /locus_tag="PK513_08615"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOO82551.1"
                     /translation="MELLAVFLPLLAFVISFLFGKQLGDKGAQFITCSALIVAAFASI
                     SLFFDVAIGENSYTTELSSWIISGDLNVSWALRIDSLSVVMMCVINIVSACVHVYSVG
                     YMSHDPCKGRFMAYLSLFTFAMLMLVTADNLLQLFFGWEGVGLASYLLIGFWNHKNSA
                     NAAAVKAFVVNRVGDFGLALAMMAIFVVFGTLQFDDIFEAVPEMAGTYFSFIGQEYHA
                     LTLIGLLLFVGAIGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVARMSPL
                     YEYAPEALMVVTLVGGATAFVAATIGLTQFDIKRVIAYSTMSQLGYMFFALGVSAYGA
                     AMFHLMTHAFFKALLFLGAGSVIHAMSDEQDMRKMGGIWKKIPMTYGLMWVGSLALAG
                     IPPFAGFYSKDIVLEAAWADHTWFGNIAFWLGIAAAFMTAFYTWRLLIMTFHGKPRAS
                     EKVMSHIHESPAVMLGPLVVLATGALFSGMVFYEPFVGSGKAEHVAEIELDAHHTLDV
                     EIEHDIEAPVLEEEGVDSGMGREKFWGQAIRVQHQNDTLESAHHVPEWVKFLPLVMGL
                     LGIGLAYLAYMLLPGIPEKIKTIFAVPYALFFNKWFFDELYNAIFIKTSFMLGRLFWR
                     VGDQNTIDRLGPDGSAKSVQKLAGVISRFQSGYVFQYAFVMMVALIAIVSWFVFKSGQ
                     GF"
     gene            complement(4854..5165)
                     /gene="nuoK"
                     /locus_tag="PK513_08620"
     CDS             complement(4854..5165)
                     /gene="nuoK"
                     /locus_tag="PK513_08620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOO82552.1"
                     /translation="MFEIALGHYLTLAALLFTIGVFGMFLNRKNVIVILMSIELMLLA
                     VNINFVAFSAYLNDLTGQVFTMFILTVAAAEAAIGLAILVAFFRNKGSIAVEDISSMK
                     G"
     gene            complement(5177..5791)
                     /locus_tag="PK513_08625"
     CDS             complement(5177..5791)
                     /locus_tag="PK513_08625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOO82553.1"
                     /translation="MIVQALAFYLFAVILTLSALMVITARNPVHSVLFLILAFFNSAG
                     LFILLGAEFVGMIMVIVYVGAVAVLFLFVVMMLDISFANLRKGAMQYVPMGLLIGAVL
                     LSELVLIYGAWGFVPGVEQNLAAPSLRFEGATNTESLGQILYTDYILAFQLAGLILFV
                     AMIGAIVLTLRARPGLRKQVVAAQHRRKRKDVLEIHKVTPGTGG"
BASE COUNT         1918 a         1796 c         1042 g         1037 t
ORIGIN      
        1 gcaacaacag atgtaagcac cccaagcgtc gccagaatgt aatatccttc agccactgcg
       61 gcgttaaata ccgccagctt gccaaagaac ccggccgcag gcggaatgcc actcatcgaa
      121 aacatcaaaa tcgccatgcc gtaggccaac cctggcgcat gccgcgacaa ccctgccaaa
      181 tcgctgattt tatacaccgc catgccgcgc cgacgcatac acatcacaat cgcaaacact
      241 ccggcactca tgatcatata aataaacaaa tacaacacca cagcgcccgc cccggccgca
      301 ctgcccgcga caacgccaat caacgcatac cccatattcc caatcgaact ataagcgagc
      361 aaccgtttga tattatcttg tgcaattccg gcaaaagcgc ccaaaaacat cgacatcaat
      421 gccaggaagt agataatctg cacccactgc tcagaaagcg ccataaacgg ctcaaataac
      481 aagcgcatca acaagcccat cgccgcaatt ttcggcacaa tcgcaaacag cgccgtcacc
      541 gccgtcggtg caccttcata aacatcgggg gtccacatat gaaacggcac agcagaaacc
      601 ttaaatgcaa gccccgccaa aacaaacacc agtccaaacg ttacgccaat cggaacaccg
      661 tccaaatcgc tcaatgttgc gcccaccaca ccaaaattta cggaacccgt aaaaccataa
      721 atcaaagaaa caccaaacaa aagcatccca gaagacaagg caccaagaag gaaatattta
      781 atccccgcct ccgacgaacg catcgaatta cgatgaaatg ccgcaagcac atacaagctt
      841 aaagactgca gctccagccc catatacagc gctaggaaat tattgcttga gaccatgatc
      901 atcatcccga tcccggcaaa caaaaccaaa atgggaaact cgaaccgctc catcccttct
      961 tgcaacaaat aacgcacaga caaagccaat gaagcaatca gaccgataat aatcatcagt
     1021 ttcataaacc cggcaaactt gtccatcaca aacataccgc caagtacgct acgcgtttcc
     1081 caacccaccg aaagcagtat caaagcacta agcgccaacc cgcagacagc tgaccagcaa
     1141 atcacacgcg tagaaacgtt gccctggctc gcaccaacga tcaacaaccc cattccaatc
     1201 aaggacagaa aaatttctgg caatacaggg atcaggttaa aatcaatcat tctcccacct
     1261 cctgcaacat gcctgtttca ggcgtttcaa caaccgctgc agcgttcaac tttgcattat
     1321 attctgtaat cagcttatcc accgacggac cgattttttc tgtcacaacg cccggaaaca
     1381 cacccagcca aagcaccaga agaacaatcg gcaccagcaa accaagctcg cgccagttaa
     1441 gatcaggcat cgagagcgca tcatcattca cacgcggtcc aaacacaacc cgacgataca
     1501 agatcagcat ataagctgcg ccaataataa cacccgtagc cgccaaagcc gcaaccaaag
     1561 tgctcacctg gaacgcacca agcaatacga gaaattcacc aacaaacccg cttgttcccg
     1621 gcaaaccaac gctgcccagc attaaaatca taaacacagt cgcatatttc ggcatatttt
     1681 tgacaatgcc gccatagcgt tcaatttcac gcgtatgcat ccggtcataa acaacgccca
     1741 cacataaaaa gagtgctccg gaaatcaaac catgcgaaag catctgaaaa atcccgcctt
     1801 caatcccttg tgtcgtcaca ctaaaaatcc cgatcgtcac aaatcccata tgcgccaccg
     1861 aggaataagc aatcaacttc ttcatatctt cctgcaccaa cgcaattagc gaggtgtaaa
     1921 taatcgcaat aacactcaaa gcaaaaacaa agtcggcaaa atacgcactc gcctcaggca
     1981 gcatcggcaa agaaaatcgt aaaaatccat aaccgcccat tttcaaaagc accccggcca
     2041 aaataaccga gccggccgtc ggcgcttcga cgtgcgcatc gggaagccac gtatgcactg
     2101 gccacatcgg cattttcacc gcaaaactcg caaaaaatgc cagccacagc caatactgca
     2161 aatgcgtcgc tactgggtta tccatcaatg tcggaatatc ggtgctgccc gtctgtgcat
     2221 agagtgccag caagcctatc aacatcaaca ccgaacccag aagcgtataa aggaagaact
     2281 tataggccga atacacacgg cgaggacctc cccatacccc gataatcaag aacatcggaa
     2341 tcaacacacc ttcgaaaaag acgtaaaaca acaccgcatc cagcgcaata aatgttccga
     2401 tcataaagct ttccagcaac agaaacgcaa ccataaattc cttaacacgt accttaactg
     2461 cgtcccacgc cgacaagata cacaccggtg tcaaaaacgc cgaaagcaag acaaaaaaca
     2521 ctgaaatacc atcaactccg acatgatagt taatccccaa aggcttcaac cagcccccct
     2581 gttcaacaaa ctgaaattca gcgcttgatc catcaaagcg cgtatacata aacagcgcaa
     2641 aagccagcgt aaacaaagat gtataaagtg ccatatgccg ggcattttgc ttcacagtcc
     2701 cttcatcccc gcgaatcatc aaaatgatca acacgccggc aaccggcaaa aacgtcatta
     2761 atgaaagaag cggaaaatct accatagtct aaaacccttg cccgctttta aatacaaacc
     2821 acgaaacaat tgcgatgagc gcaaccatca tcacaaatgc atactgaaag acatatccgc
     2881 tctggaaacg gctaatcaca ccagccagct tttgaacgct cttcgcactg ccgtccgggc
     2941 ccaaccggtc aatcgtattt tgatccccaa cgcgccagaa gagccgtcca agcataaaag
     3001 aggtttttat aaaaatagcg ttgtacagct catcaaagaa ccacttattg aagaaaagcg
     3061 cataaggcac agcaaaaata gttttgattt tttcaggaat tcccgggagc agcatatacg
     3121 ccagataggc taaaccgatc cctaaaagcc ccataaccaa cggcaggaac ttgacccatt
     3181 caggcacatg gtgggcagat tccagcgtat cattttggtg ctgcacacgg atagcctgtc
     3241 cccagaattt ttctcttccc atgccgctat ccacgccttc ttcttcaaga acaggtgctt
     3301 caatgtcatg ctcaatttca acatcaaggg tgtgatgcgc atcgagttca atctcagcaa
     3361 catgctcagc cttaccgcta ccgacaaacg gttcatagaa gaccatcccg gaaaacaatg
     3421 cgcctgttgc caaaacaacg agcggcccca gcatcaccgc cgggctttca tgaatatgac
     3481 tcatcacttt ttcactcgcc cgcggcttgc cgtggaacgt catgattaac aagcgccacg
     3541 tataaaacgc cgtcataaag gctgctgcga tccccagcca gaaggcgata ttcccaaacc
     3601 atgtgtgatc cgcccatgcg gcctccagca cgatgtcctt ggaataaaat ccggcaaaag
     3661 gcggtatccc ggccagcgca agcgacccaa cccacatcaa accataggtc atagggattt
     3721 tcttccaaat cccgcccatc ttgcgcatat cctgctcatc actcatcgcg tgaatgaccg
     3781 aaccggcccc caggaacaac aaagccttaa agaacgcatg cgtcatcaaa tgaaacatag
     3841 ccgcgccata ggccgacacg cctagcgcaa agaacatata gccgagctgg ctcatcgttg
     3901 aatacgcaat gacccgcttg atatcaaact gcgtcagacc gatcgtcgcc gcgacaaacg
     3961 ccgtcgcccc gccaaccaat gtcaccacca tcagcgcttc tggagcatat tcatacaagg
     4021 gtgacatccg cgccaccagg aacacaccag cagtcaccat cgtcgccgcg tgaataagcg
     4081 ccgaaaccgg cgtcggcccc tccatcgcat caggaagcca cgtatgcaac cccaattgcg
     4141 ccgactttcc gatcgccccg acaaacaaca acaaaccaat cagcgtcaac gcgtgatatt
     4201 cctgaccaat aaagctgaaa tacgtcccgg ccatttccgg aactgcctca aaaatatcat
     4261 caaactgcaa cgtaccaaac acgacgaaaa tcgccatcat cgccagcgcc agcccaaaat
     4321 caccaacccg gttcacaaca aaagccttca ccgctgcggc attcgcactg ttcttatgat
     4381 tccaaaaccc gattaacagg taggaagcca acccgacccc ctcccagcca aagaacagtt
     4441 gcagcaaatt atccgccgtc accaacatca acatcgcaaa ggtaaacagc gacagatacg
     4501 ccatgaaccg acccttgcag ggatcatggc tcatataccc gaccgaatac acatgaacgc
     4561 acgccgatac gatattaatc acgcacatca tcaccacaga cagcgaatca atccgcagcg
     4621 cccatgaaac atttaaatca cctgaaataa tccaacttga aagctcagtc gtatagctat
     4681 tttcaccgat agccacatca aaaaacaggc taatcgaggc aaaagccgcc acgatcaaag
     4741 cactacatgt tataaactga gcgcctttat ccccgagctg ctttccaaac agaaaagaaa
     4801 tcacaaacgc aagcaaaggc aaaaagacag ctaaaagttc cattacacgc gccctacccc
     4861 ttcatactcg aaatatcttc gaccgcgata gaacctttat tgcggaagaa cgccaccaaa
     4921 attgccagcc cgatcgccgc ttcagccgcc gccaccgtta agataaacat cgtaaagacc
     4981 tgccccgtca aatcattcag ataggcggaa aacgccacaa aattaatatt caccgcaagc
     5041 agcatcaatt cgatcgacat caaaatcaca atcacgtttt ttcggttcaa aaacatgcca
     5101 aacacaccga tcgtaaaaag caacgccgcc aaagtcagat aatgtcccaa agcaatttca
     5161 aacatgccgc tccccattag ccccccgttc ccggcgtaac cttgtgaatt tccagcacat
     5221 ccttacgctt gcgccgatgc tgggccgcca ccacctgctt gcgcaaacca ggacgagcgc
     5281 gcagcgtcag aacaatcgca ccgatcatgg ccacaaataa aattaaaccg gcaagctgaa
     5341 acgccaaaat ataatccgta tataaaatct gccccaaact ttcagtattc gtcgcgcctt
     5401 caaaacgcaa agaaggcgcg gctaaatttt gctcaacccc tggaacaaaa ccccaggcac
     5461 cataaatcaa caccaactca ctcaatagaa ctgcgccaat caataatccc atcggcacat
     5521 attgcatcgc ccctttacgc aaattagcaa aggaaatatc aagcatcatc acaacaaaca
     5581 agaacaacac cgccaccgcg cccacataga caatcaccat gatcatcccg acaaattcag
     5641 cccctaataa tatgaacaat ccggcagaat taaaaaacgc aagaatgaga aacagtacgg
     5701 aatgcacagg gttgcgcgcc gtaatcacca tcagggcgct taaggttaaa atcacggcaa
     5761 agaggtaaaa ggcaagggcc tgaacgatca ttg
//
LOCUS       DAOSWR010000124         5777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_9216_length_5777_cov_5.728766, whole genome shotgun sequence.
ACCESSION   DAOSWR010000124 DAOSWR010000000
VERSION     DAOSWR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9216_length_5777_cov_5.728766"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..2781
                     /gene="clpB"
                     /locus_tag="PK513_08630"
     CDS             154..2781
                     /gene="clpB"
                     /locus_tag="PK513_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOO82554.1"
                     /translation="MDFEKFTEKSKGFIQAAQTLAMRYGHQSIEPEHLLKVMLDDSDA
                     LVSKLVKAADGNLARLKAGLETAIAKFPVVEGSGSQLRISTDFAKTLDSALNLAEKAG
                     DKYVTAERILQAMVLAKKLDIAGYLEDAGLSDTKLNQAINDMRKGRTADSASAEDQFD
                     ALNKYAHDLTAAAQDGKMDPIIGRDEEIRRTVQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVNGDVPESLKGKRLMSLDLGALLAGAKFRGEFEERLKAVLDEVKAATGEIVL
                     FLDELHTLVGAGKAEGSMDAGNMLKPALARGELHMVGATTLDEYRKHIEKDAALARRF
                     QPVFVSEPTVEDTISILRGLKEKYELHHGVRITDTAIVAAATLSNRYITDRFLPDKAI
                     DLVDEAASRIRMQVDSKPEALDELDRRIIQLKIEREALKKEKDKASKERLIKLEEELK
                     SLESQSADLSAQWAAEKESLNAGQKHTESLDKARIELEKAERDGDWAKASELKYSTIP
                     ELEQKIAEAVENGSGQGESKTTSSLINEEVTEDDIAGVVSKWTGVPVDKMLEGEREKL
                     LEMENKLRQRVVGQDEAVTAVSNAIRRARAGLQDPRRPMGSFLFLGPTGVGKTELTKA
                     LAEFLFDDDSAMIRMDMSEYMEKHSVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVL
                     FDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFSNTLLIMTSNLGAEHLVAQKDGAD
                     VEEVHSLVMEAVRAAFRPEFLNRLDEILLFKRLDREQMTGVVEIQLRYLHALLDGRHI
                     TLEIDDKAKTWIGDQGYDPAYGARPLKRVIQTALQNKLAEMILAGNIMDGSSVKISVK
                     GDALDFKVENPAGAKKSAA"
     gene            2896..3582
                     /locus_tag="PK513_08635"
     CDS             2896..3582
                     /locus_tag="PK513_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82555.1"
                     /translation="MKKTLLLLSVLSTGACAYAIDDSIQDVKIVTPGARGAACNAYVE
                     GLKYRVKPPQIINLYKSKEDLVVDCKAPGNRRKVVYIKPSIEPSAAWNITNAGVGLTW
                     DYASAALFRYPDVIEVNFTDTPVTDPPLPAQNSPDIRQPEEYQLEEFSPSAPRMNADR
                     SAPPVEILRRERASRTMSGYDSSAAFSEPSALDGGKGDLMDIIQDMRSDLNPASDNSA
                     DAPISLLPGQ"
     gene            3588..4994
                     /locus_tag="PK513_08640"
     CDS             3588..4994
                     /locus_tag="PK513_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOO82556.1"
                     /translation="MNDKLSKSYFFCGIGGSGMAPLAAIMAAKGENVSGSDRAYDRGQ
                     SPEKFEKLQKAGITLFPQDGSGLSAATDTLIVSSAVEETIPDVKAALDQGIPIQKRAE
                     LLASLFNSYESGISIAGTSGKSTVTGMSAVMLAELDLDPTVMNGGAIRNFETMPEGGQ
                     SLPSMRIGTSDIFVTETDESDGSIALYEPAIAVVNNIALDHKPVEELTALFKSFIGRA
                     RHAVVLNFDDQALRDMTPYASAPVYSYAITDESADLTAHNIKYQSAGVRFTAKCDGQS
                     HNVTLQVPGAHNVSNALAALSIARAMDIALQNAIPALEKFTGIARRLAVTGIRAGITV
                     IDDFAHNPDKISASLKTLKAFDGRLLVVYQPHGFGPLKLMGREIIESFAVHLTSGDQL
                     LMPEVYYAGGTADRSVTSKDVIGWAKEKGIDAHWFAMRDEILPYIKDHAREGDRIIVM
                     GARDDTLTDFAYKILDQV"
     gene            complement(4998..>5777)
                     /locus_tag="PK513_08645"
     CDS             complement(4998..>5777)
                     /locus_tag="PK513_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOO82557.1"
                     /translation="GKASPRAPRLIEMVWGNAKHPKVTLVGKGVAFDTGGLDIKPSSS
                     MALMKKDMGGAAHVIGLAKLIMDHGLPVRLRVLIPAVENVVGGAAYRPSDIIKSRKGV
                     FVENTNTDAEGRLILADALAYACEDKPELVVDYATLTGSARAGLGPDIPAMFSNNEEV
                     AQDIQKLAKKTEDPVWNMPLWQPYRKYLDSSNADIINSTGQAGDGIYSALFLETFVEK
                     DTDWVHLDCFSWEMSGRPGRPKGAADTGLRTVFAYLQGRFG"
BASE COUNT         1454 a         1561 c         1453 g         1309 t
ORIGIN      
        1 cagaggcgtc tgtgtaatat acaggttgta tgtgtctgat ttaaaggcgg ttttttgggc
       61 cgccttttta atgtttaaca cagaaaaatt cttcctttcg aaacttgact aaaatcattg
      121 aaaagttaca gcggaattct ataattcctc attatggatt ttgaaaaatt tacagaaaag
      181 tcaaaagggt ttattcaagc ggcgcagaca cttgcgatgc gctatgggca ccaatcaatt
      241 gagccggagc atctgcttaa ggttatgctt gatgattcag atgctttggt ttccaagctt
      301 gtgaaagccg ctgacggtaa tcttgctcgc ctcaaagcgg gtctggaaac cgcgattgcc
      361 aaattcccgg ttgttgaagg ctccggctca cagctccgga tttcaaccga ttttgcaaaa
      421 acacttgata gtgcattgaa tttagctgaa aaggcgggtg acaaatatgt cacagcagag
      481 cgcattctgc aggccatggt gctggccaaa aaactcgata ttgccggcta tctcgaagat
      541 gcaggcctca gtgacacaaa gctcaatcag gccattaacg acatgcgcaa aggccgcact
      601 gccgatagcg ccagtgctga agaccagttc gacgcgctta acaaatatgc tcatgacctc
      661 acagccgccg cgcaagacgg caaaatggac cctatcattg gccgtgatga ggaaatccgc
      721 cgcacggtgc aggtgctctc tcggcgtacc aaaaacaacc ccgttctcat tggtgagccc
      781 ggtgttggta aaaccgccat cgttgaggga ctggctcaac gcatcgtcaa tggtgatgtg
      841 cccgaaagcc ttaaaggaaa acgcctgatg tcgctcgacc ttggcgcact gctggccggt
      901 gcgaaatttc ggggcgaatt cgaagagcgt ctcaaagccg tgctggatga agtcaaagcc
      961 gccactggtg aaatcgttct gtttctcgat gagcttcaca cgctcgttgg tgcaggcaag
     1021 gccgaaggct caatggatgc cggcaatatg ctcaaacccg ctttagcgcg cggtgagctg
     1081 catatggttg gcgccacaac gctggatgaa taccgtaaac atatcgaaaa agatgccgcg
     1141 ctcgcccggc gtttccaacc ggtttttgtt tccgaaccaa cggttgaaga taccatctcc
     1201 atcttgcgcg ggctgaaaga aaaatacgag cttcaccacg gtgtacgcat cacagacacg
     1261 gccattgttg ctgcggccac gctgtccaac cgctatatca ccgaccgctt cctgcccgat
     1321 aaggcgattg atctggttga cgaagccgcc agccgcattc gcatgcaagt tgattccaag
     1381 ccagaagcgc tcgatgagtt ggaccggcgc ataatccagc tcaaaatcga acgcgaagcg
     1441 cttaagaaag aaaaggataa agcctccaaa gaacgcctca taaagcttga ggaagagcta
     1501 aaatctctcg aatctcaaag cgccgatctg agcgcgcaat gggcggctga aaaggaaagc
     1561 ctgaatgccg gacaaaaaca cacagaatct ctagataaag cgcgtataga gctggaaaaa
     1621 gctgaacgcg acggcgactg ggccaaggcc agcgaactga aatattccac tattccggaa
     1681 ctggaacaaa aaatcgcgga agctgttgaa aatggaagcg gacagggcga atctaagacc
     1741 acctccagcc tcattaatga agaggttacc gaagatgata ttgctggtgt ggtcagcaaa
     1801 tggaccggtg tgcctgttga taaaatgctc gaaggcgagc gtgaaaagct tcttgagatg
     1861 gaaaataaac tccgccagcg cgttgtgggg caggatgaag ctgttacggc cgtgtccaac
     1921 gccattcgcc gcgcccgcgc agggctgcaa gacccgcgcc gcccgatggg ttcattcctg
     1981 tttctcggcc ccaccggcgt tggaaaaacc gaactaacca aggctttggc cgaatttctg
     2041 ttcgatgacg acagcgcgat gatccgcatg gatatgtcag aatatatgga gaaacactcg
     2101 gtcgctcgcc tcataggcgc accgccgggt tatgtcggct atgaggaagg cggcgcactg
     2161 accgaagccg ttcgccgccg tccatatcag gttgtgctct tcgacgagat tgaaaaggcc
     2221 cacccggacg tattcaacgt tctgctgcaa gtccttgatg atggccgcct gacagacggg
     2281 cagggccgta cggttgattt ctctaacacg ctactcatca tgacctcgaa tctgggagca
     2341 gagcatctgg tcgcgcaaaa agacggggct gatgtagaag aagttcattc cctggtaatg
     2401 gaagccgtgc gtgccgcctt ccgcccagaa ttccttaacc gtctcgacga aattctgctg
     2461 ttcaagcgtc tggatcggga gcaaatgaca ggcgtcgtag aaatacagct ccgctatctt
     2521 catgctctgc tggacggtcg ccatatcacg ctggaaattg acgacaaggc aaaaacatgg
     2581 atcggggatc agggctacga tccagcctat ggcgcccgcc cgctcaagcg tgtaattcaa
     2641 accgcgctgc agaataaact tgctgagatg attctcgccg ggaatattat ggatggctca
     2701 agcgtaaaaa tctccgtgaa gggcgacgct ctcgatttca aggtcgaaaa tccggctggg
     2761 gccaagaaat ctgctgcata acgcgaatgg ctcgctgcgc ttgtcctctc atgttgcctc
     2821 gcgcgctgat ttccggtaga tttatcaaga cactcgagcg tcccgccctt tgctttatat
     2881 gtaagtccag accctatgaa aaaaacatta ttacttctat ccgttttatc cactggcgca
     2941 tgtgcctacg ccatagatga ttcaattcaa gacgtaaaaa ttgtcacgcc cggtgccaga
     3001 ggcgctgcct gcaacgccta tgttgaaggg ctgaaatatc gtgtaaagcc gccccaaata
     3061 atcaatcttt ataaatccaa agaggatttg gtggttgatt gtaaggcacc gggcaatcgc
     3121 cggaaggtcg tttatatcaa gccatccatt gagccgagtg cggcgtggaa cattaccaat
     3181 gcaggcgttg gtttaacttg ggattacgca tccgctgcgc tcttccgtta tcctgatgtg
     3241 attgaggtaa acttcactga taccccggta accgacccgc cgctgcctgc gcaaaatagt
     3301 cctgatattc gccagcctga ggaataccag cttgaggaat tttcgccatc tgcaccgcgc
     3361 atgaatgcag atcgttctgc gccgcctgtg gagattctcc gccgtgaaag agcctctcgg
     3421 actatgagcg gctatgacag ctcagcggct ttctcagagc caagcgcttt agatggcggg
     3481 aaaggtgatc tcatggatat aattcaggat atgaggagcg atttgaatcc cgcatcagac
     3541 aatagtgcag atgcgcctat ttcgcttttg cctgggcagt aggctccgtg aacgataagc
     3601 tatccaaaag ctattttttc tgcggtattg gtggatctgg catggcgcct ttggcagcaa
     3661 ttatggctgc gaagggggaa aatgtcagtg gttccgaccg cgcctatgat cgcgggcaaa
     3721 gccccgaaaa atttgaaaag ctgcaaaagg ctggtatcac actttttcct caggatggct
     3781 ccggtctaag cgctgccaca gacacgctta ttgtctccag cgccgtcgaa gaaaccatcc
     3841 ccgatgttaa agccgcgctt gatcaaggca ttcccatcca aaaacgcgcc gaattgctcg
     3901 ccagcctgtt taacagctat gaatccggca tctccatagc cggaaccagt ggcaaatcca
     3961 ctgtcaccgg catgagcgcc gtaatgcttg ctgaattgga tttagaccca acagttatga
     4021 atggcggcgc gatccgtaat ttcgaaacca tgccggaagg tggccaaagt ctcccatcca
     4081 tgcgtattgg caccagtgac atcttcgtca ccgaaaccga tgaaagcgat ggttcaatcg
     4141 cgctgtatga acccgccatt gccgttgtga acaatatcgc tctcgatcac aagcctgtgg
     4201 aagaactcac agcgctcttc aaaagcttca tcggcagggc gcgccacgcc gttgtgctga
     4261 atttcgatga ccaggccctg cgggatatga ccccttatgc cagcgcgccg gtttatagct
     4321 acgctatcac tgacgaatct gcggacctca ctgcgcacaa tatcaaatac caatctgctg
     4381 gcgtgcgttt caccgcaaag tgcgacggcc aaagccacaa cgttacgctg caagttccgg
     4441 gtgcgcataa cgtctccaac gcgctcgctg cgctcagcat cgcccgcgcc atggatatcg
     4501 cactacaaaa tgctattccc gcccttgaaa aattcaccgg aatcgcccgc cgtttggccg
     4561 taaccggcat tcgcgccggg attaccgtaa tcgatgattt cgcccataac ccggataaaa
     4621 tatccgccag cctcaaaaca ctcaaagctt tcgatgggcg gttgttggtg gtgtaccagc
     4681 cccatggctt tggtcccctc aaactcatgg gccgcgagat tatcgagagc ttcgctgtgc
     4741 atcttacctc cggcgatcaa ctcctcatgc ccgaagtcta ttacgctggc ggcaccgccg
     4801 atcgcagcgt tacttccaaa gatgttatcg gctgggcaaa agagaaggga atagatgcgc
     4861 attggttcgc tatgcgcgat gaaatcctgc cttatataaa agaccatgcc cgcgagggtg
     4921 atcgcattat tgtgatgggt gcgcgtgatg acacactcac cgactttgcc tataaaatct
     4981 tagatcaggt ctagcactta cccaaaccgt ccctgcaaat acgcaaagac cgtgcgcaga
     5041 ccagtatctg ccgcgccttt ggggcggccc ggacgacctg acatttccca tgaaaagcaa
     5101 tccagatgca cccaatctgt atctttttca acaaaagttt caaggaataa cgcgctgtaa
     5161 attccatcac ctgcttgccc tgtgctgttg attatatcag cattcgaact gtctagatat
     5221 ttacgataag gctgccacaa aggcatattc caaactggat cttcggtttt tttcgccagc
     5281 ttttgaatat cttgagcaac ttcttcatta ttcgaaaaca tcgccggaat atccggcccc
     5341 agccctgcgc gcgctgaacc cgtcagcgtc gcgtaatcca ctaccaactc cggcttgtcc
     5401 tcgcacgcat aggccagcgc atcagccaga ataaggcggc cttccgcgtc tgtattagta
     5461 ttttcgacaa aaaccccctt gcgcgatttt ataatatcgc ttgggcggta agccgcgccg
     5521 cccacgacat tttccaccgc cggaataagc acccgtaggc gaaccggcaa cccgtgatcc
     5581 ataatcagct tggccagacc gataacatgc gccgcaccgc ccatatcctt tttcataaga
     5641 gccattgagg atgaaggttt tatatccagc ccgccggtgt caaacgccac gcccttgccg
     5701 accagcgtca ctttcggatg tttcgcattg ccccagacca tctcaatcag acggggggcg
     5761 cgcggactgg ctttacc
//
LOCUS       DAOSWR010000125         5706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_9384_length_5706_cov_5.442400, whole genome shotgun sequence.
ACCESSION   DAOSWR010000125 DAOSWR010000000
VERSION     DAOSWR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_5706_cov_5.442400"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK513_08650"
     CDS             <1..431
                     /locus_tag="PK513_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO82558.1"
                     /translation="SWLEDILRLAGTLKLSALIVTLIIGATTIVAIAGAIRSRMAEHK
                     ADIELLHLMGASDLYITKQLQRHAVVLTLKGALIGVLSGLMILLMLSAFGGSGEGATI
                     PSMHLSTSQIIILLLLPGLIALIASQTARVTVLRVLGQMP"
     gene            477..1052
                     /locus_tag="PK513_08655"
     CDS             477..1052
                     /locus_tag="PK513_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOO82559.1"
                     /translation="MALILLWIGGFCVFAASAIMATPQSEEETTDAIVVLTGGKNRIQ
                     EGLSLFAQGRALHLFISGVHEDVKKYEILALWDGDHALPPCCVSLGYEATTTTQNAAE
                     TRKWIQKQDYSSIRLVTGDYHMMRSLMEMHHALPGVDIYAHPVHQTDFTIDTLHYWEL
                     LFSEYHKCMYRWLQLLFTPRIELLNFSELAK"
     gene            1056..2597
                     /locus_tag="PK513_08660"
     CDS             1056..2597
                     /locus_tag="PK513_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOO82560.1"
                     /translation="MKRAVIRSSVFNVLFYALTALACVLCLPTLALPRRYFMGIVKFF
                     VHKVYFLERFILGLDYEVRGSKHLPESGSFIVAAKHQSAYETMKLHILFKDPAVILKK
                     ELLSIPLWGLYLKKSDPIAIDRSSPDTAIASIQEGARRMKVLGRPIVIFPQGTRVHTD
                     SSATDKPYKVGVARLQEATDLPIIPMALNAGIFWPRNSWLKSSGCVIFEFLKPIKPGL
                     ERGRLLAKLEKETENVVQSLMNEAKEKALDTKPAGRRAAFWALFILGLAFGLYSFVWF
                     KAADQIKNEYVGALSDLTDAQTPVMPPMIAGYPGKLRLHKAEEMIVTDEGSVKVHDLG
                     AIGWPLPFLPITVTSGPIEIQNFKWGSALHFDSLFAKIKYDRDILNVYESALVQGDFT
                     GTLTGTADLKQEPVPALDMIIQFTNHQSLLQSLVASDIIETRMALFIGAGLSSLADES
                     GLISLPLQQKGETLYAGPLPIMTIRTENKNIRRKTKPPTPNPASLEPQEIPAMDLEEP
                     LHPSR"
     gene            complement(2518..3459)
                     /locus_tag="PK513_08665"
     CDS             complement(2518..3459)
                     /locus_tag="PK513_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HOO82561.1"
                     /translation="MSTVMFEKITIIGLGLIGSSLARVIKRKDLTQKLVAADTSESVC
                     TKVRELGLADVVTPDITKSVIGSDLVILAVPVGAVRAVIEQIAPLLMPGAVLTDVCSV
                     KSAVIEAAAPFLPESADLVPAHPIAGTEHSGPESGLVDLFDGRWCILTPPPETSIKAV
                     EKVTKFWEACGAQIEIMDAAHHDLVLGITSHLPHLIAYTIVDTADQLEDDLKAEVIKY
                     SASGFQGFTRIAASDPIMWRDIFMNNKEAVLEILQRFTEDLTAMQKAIRRGDGSYLQE
                     TFIRTRAIRREIIEMGAKVLPNPSLVSLEVPKKQDSE"
     gene            3531..4586
                     /locus_tag="PK513_08670"
     CDS             3531..4586
                     /locus_tag="PK513_08670"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOO82562.1"
                     /translation="MPLVAYKDLPAFKRLRKEGRTVLSPERAQSQEIRELHIGLLNMM
                     PDAALQATERQFFRLIGESNQIAQFYVHPFTLPELARSTETQSYIDQYYESFDQIKTD
                     GLDGLIITGANVSDPDLSKAPFWEPLQEVISWAWENVTSTLCSCLATHAVMQFQYAQT
                     RTPMDKKLWGVYPHRVLDRNHPLMRGVNTVFDVPHSRYNELTQEQFEAAGLKVLSVSD
                     DGDVHMAVSPDGFRQLLFQGHPEYDTISLYKEYEREVKRFIAKECANYPPFPDHYFND
                     DLKKLMKQLAAAVKRGQTDWPLTDDDMRQSIENSWGDSARSIIANWIGLVYQITNMDR
                     TKPFMNNVDPDHPLDGL"
     gene            complement(4604..5356)
                     /locus_tag="PK513_08675"
     CDS             complement(4604..5356)
                     /locus_tag="PK513_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO82563.1"
                     /translation="MYESVYDFKTFYNTRIGRIVRRVLQERIREIWPDVHGLSLMGIG
                     YATPYLRIFREEAERVFAIMPAGQGAHDWPHEGPNCVALAEQAELPIETNSVDRILMV
                     HNLEFTESPKPALQEIWRVLKSNGRLLVVTPNRAGLWARTEHSPFGHGTPYSLTQLTH
                     ALRDNLFVHETTEEALFVPPLKYSTLLKSAGFFERYGRSILPVVAGVHIVEASKQIYA
                     SADRSPGSTVRIRGRGVFMPKPVPNNFNKDNA"
     gene            complement(5367..>5706)
                     /locus_tag="PK513_08680"
     CDS             complement(5367..>5706)
                     /locus_tag="PK513_08680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013963987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOO82564.1"
                     /translation="ARRPEGKPMAGLWEFPGGKVDEGETPEFALMRELEEELGIETRP
                     TCYHPIAFASHSYDDFHLLMPLFACRKWRGDITLNEHQDGAWVKPVDMLDYPMPAADL
                     PLVTTLRDLL"
BASE COUNT         1458 a         1304 c         1506 g         1438 t
ORIGIN      
        1 aaagctggct ggaggatatt ttacgcctgg ccggaacatt gaaactttcg gcgttaattg
       61 taacgctgat tattggcgcg acgacgattg tggcgattgc tggagcaatt cgttcgcgca
      121 tggccgagca taaagccgat attgaattgt tgcatttgat gggggcgagc gatctgtata
      181 tcaccaagca actccagcgc catgcggtgg tcttaacact gaaaggagct ttgatcggtg
      241 tgctcagcgg attaatgatt ttgcttatgc tttcagcttt tggaggttct ggcgaagggg
      301 caactattcc tagtatgcac ctaagcactt cacaaatcat aattttgctg ctcttgccag
      361 ggctgattgc tcttatcgca tcacaaacag cgcgtgttac agtcttgcgt gtgttggggc
      421 agatgccgta aaagaaacag gtaatgcaaa tatttaaacg tgtttttttg agcttcatgg
      481 cgctgattct tttatggatt ggtgggtttt gcgtttttgc agcttcggcc attatggcca
      541 cgcctcagtc tgaagaggaa acaaccgatg ccattgtcgt gcttacaggt ggaaaaaacc
      601 gtattcagga agggctatcc ctatttgcac agggacgtgc tttacatctg ttcatcagcg
      661 gtgtgcatga ggatgtaaaa aaatatgaaa ttctggcatt atgggatgga gatcacgccc
      721 tgccaccatg ctgcgtcagc cttggatatg aagccacaac aacgacacag aatgctgccg
      781 aaacacggaa gtggattcaa aaacaagatt acagttcaat ccggttggtg accggtgatt
      841 atcacatgat gcgatctctt atggaaatgc atcatgccct gcctggcgtt gatatttacg
      901 cgcatccggt gcatcaaact gattttacca tcgacacgct tcattactgg gaattgctat
      961 ttagcgaata ccacaagtgc atgtatcgct ggctacaatt gcttttcact cccagaatcg
     1021 aacttttaaa cttctcagaa ctggcaaaat aactcatgaa gcgcgccgtt attcgttcat
     1081 ccgttttcaa tgtgctgttt tatgcgctga ccgctttggc gtgcgttttg tgtttaccaa
     1141 cactggcgct gccccggcga tatttcatgg gcatagtgaa attctttgtt cacaaggtct
     1201 attttctaga acggtttatt ctggggctgg attacgaagt gcgggggagt aaacatctgc
     1261 cggagagtgg ttcgttcatc gttgcggcca agcatcaatc agcatacgaa acaatgaagc
     1321 tgcatattct gtttaaagat ccggcggtga ttttgaagaa agagcttctt tcgattccgc
     1381 tttgggggct ttacttaaaa aagagtgatc caattgcaat cgaccgaagt agcccggata
     1441 cggcgattgc ctccattcaa gaaggcgcgc ggcgaatgaa ggtgctgggc cggccgattg
     1501 ttatttttcc gcaaggtacg cgcgtacata ctgattccag tgcgacagac aagccataca
     1561 aggtcggcgt agcgcgctta caggaagcta ctgatttgcc gattattccg atggcactca
     1621 atgccggaat tttttggccg cgtaatagct ggctcaaatc ttcgggctgc gttatctttg
     1681 aatttctcaa acctataaaa ccgggactag aaagaggcag gctgttggca aagttagaaa
     1741 aagaaacgga aaatgtggtg caaagcctga tgaacgaagc caaagagaaa gcgctggata
     1801 cgaaacccgc cggtcgaagg gcggcatttt gggcgctgtt tattttggga ctggcgtttg
     1861 gcttgtatag ttttgtgtgg tttaaggcag cagatcaaat caagaatgag tacgtgggcg
     1921 cgcttagtga tctcacggat gcgcaaaccc ctgttatgcc acccatgatc gccggttatc
     1981 ctggtaaact ccgtcttcat aaggcagaag aaatgattgt taccgatgaa gggagcgtga
     2041 aggttcacga tttaggtgcc ataggttggc ctttgccgtt tttgcctatc accgtgacca
     2101 gcggaccaat cgagattcag aatttcaaat ggggcagcgc tttgcatttt gactccctgt
     2161 ttgccaagat caaatatgac cgcgatattc taaacgttta tgaaagtgca ctggtgcagg
     2221 gtgattttac gggcacactc accggaacgg cagatttaaa acaagagccc gtaccggcgc
     2281 tggatatgat cattcaattc accaatcatc agagcttgct acaaagtctg gttgccagcg
     2341 atattattga aacacgcatg gcgctgttta taggcgcagg gctaagctct ctggctgatg
     2401 aaagcgggct tatcagcctg ccgttacagc aaaaaggcga gacgctttat gcggggccat
     2461 tgccgattat gacgatccgt acagaaaata aaaatatccg gcgtaaaacc aaacccccta
     2521 ctccgaatcc tgcttctttg gaacctcaag agataccagc gatggatttg gaagaacctt
     2581 tgcacccatc tcgataattt cgcggcggat ggcacgtgtg cggataaatg tctcctggag
     2641 atagctccca tcgccacggc ggattgcctt ttgcattgcg gtaaggtcct cggtaaagcg
     2701 ctggagaatt tcaagtacgg cctctttgtt gttcataaat atatcgcgcc acatgatggg
     2761 gtcggatgca gcaatgcggg tgaaaccctg aaacccggag gcagaatatt taatcacttc
     2821 agccttgaga tcatcttcga gctggtcggc ggtatcgaca atcgtgtagg cgatcaggtg
     2881 cggcaagtgt gaggtaatgc cgagtacaag atcgtggtgg gctgcatcca taatttcaat
     2941 ttgtgcgccg caagcttccc agaattttgt aaccttttcc acggctttga ttgaggtttc
     3001 cggtgggggc gtaagaatac accagcgccc gtcaaataaa tcgaccaggc cagattcggg
     3061 gccggaatgc tcagttcccg caataggatg agcgggcaca agatcagcac tttctggcaa
     3121 aaatggcgcg gcagcttcaa tcacggcaga tttaaccgaa cagacgtcgg tgagcacagc
     3181 gccgggcatt aacaaaggag ctatttgttc aataaccgct cgcactgcac cgacaggcac
     3241 ggccagtatc acaaggtcgc tgccaatcac gcttttggtt atgtcggggg tgacaacatc
     3301 ggcgaggcca agttcacgaa cctttgtaca aacgctttcg cttgtatccg cagcaacaag
     3361 cttttgagtt agatctttcc gcttgattac gcgtgccagt gacgagccga tcaaacccaa
     3421 gccgataatg gtaatttttt cgaacattac agtactcata ctctttgcct ttagcggata
     3481 ttcgcgtaca aaagacatat agatagcatg ttaaaaaatc aaagggaact atgccgctcg
     3541 ttgcctataa agatttaccc gcatttaagc ggcttcgtaa agaaggacgt acagtgcttt
     3601 cgccggagcg ggctcagagt caggaaattc gcgaactgca tattggtttg ctcaacatga
     3661 tgcctgatgc ggcgttgcaa gccacagagc ggcaattttt tcgtctgatc ggggaaagta
     3721 atcagattgc gcagttttat gtacaccctt ttacgctgcc ggaattagcg cgcagcacgg
     3781 agacgcaaag ctatatcgat caatattatg aaagttttga tcagatcaaa accgacggcc
     3841 tggacgggct aattattacg ggtgcaaatg tcagtgatcc ggatttgagt aaggccccct
     3901 tttgggagcc gttgcaagag gtgatcagct gggcgtggga gaatgtgact tcaacgctgt
     3961 gttcgtgcct tgccacgcat gcggtgatgc aatttcaata tgctcagaca cgaaccccga
     4021 tggataaaaa actctggggc gtttatcctc accgggtgct ggatcgcaat catccgctaa
     4081 tgcgcggggt gaatacggtt tttgatgtcc cgcattcgcg ctataacgaa ctcacgcaag
     4141 aacagtttga agcggcaggc ttaaaggttt tgagcgtgag tgatgatggt gatgtgcata
     4201 tggccgttag cccggacgga tttcgccaac tcctatttca ggggcatccg gaatatgaca
     4261 cgatcagcct gtataaagaa tatgagcgtg aggttaaacg cttcatagcg aaagaatgtg
     4321 ctaactatcc tccctttcct gatcattatt ttaatgatga tctcaaaaag ctgatgaaac
     4381 agcttgcggc ggcagtgaaa cgcgggcaaa cagattggcc gctcaccgat gatgatatgc
     4441 gccagagcat tgaaaatagc tggggggata gcgcacgctc cattatcgca aactggatcg
     4501 ggctggtgta tcaaatcacc aatatggatc gtacaaagcc gtttatgaat aatgttgatc
     4561 cggaccatcc tttggatggg ctttaagaat caagacctag atgtcaggca ttatctttat
     4621 tgaaattatt gggtacaggt ttgggcataa aaactccacg gccacgtata cgcacagtcg
     4681 aaccggggct acgatcggcg ctggcgtaga tctgtttgct ggcctcaacg atgtgaacac
     4741 cagcgacaac cggcaagata ctacgtccgt agcgttcgaa aaaaccggcg gatttaagca
     4801 gagtggaata tttaagcggt ggaacaaaca acgcttcttc ggtcgtttca tgtacgaata
     4861 aattatcgcg caaggcatgc gtgagttggg tgagtgaata tggcgtacca tgaccaaagg
     4921 gagaatgttc agtgcgcgcc cacaagccgg cgcggttcgg cgtaacaaca agcaggcggc
     4981 cattggattt aagaacgcgc cagatttcct gcaaggcggg cttgggggat tccgtgaatt
     5041 ccagattatg aaccatcaga atacggtcta cgctgttggt ttctatggga agttcggcct
     5101 gctcggccag cgcgacacag tttggtcctt catggggcca atcgtgcgcg ccttgcccgg
     5161 ctggcattat cgcgaagaca cgctctgcct cctcgcggaa aattcgtaaa tacggcgtag
     5221 cgtagccgat ccccatgagt gagaggccat gcacatcagg ccaaatctcg cggatacgtt
     5281 cctgaagaac gcgccggaca atgcgcccga tgcgggtatt gtaaaaggtt ttgaagtcat
     5341 aaacgctttc atacataagc gttaatctac agcaaatcgc gcagtgttgt caccaaggga
     5401 agatcggcgg caggcatagg ataatccagc atatcgacgg gctttaccca ggcgccgtct
     5461 tgatgctcgt ttagtgtgat gtctccacgc catttgcggc aggcaaaaag cggcatcaga
     5521 aggtgaaaat catcatagct gtgcgaggca aaagcgatgg ggtgataaca agtgggccgc
     5581 gtttctatgc cgagttcttc ttccaattcg cgcatcaggg caaattccgg ggtttcgccc
     5641 tcgtcaacct tgccgccggg aaattcccac agaccggcca tgggtttgcc ttcggggcgg
     5701 cgggca
//
LOCUS       DAOSWR010000126         5538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_9776_length_5538_cov_5.224695, whole genome shotgun sequence.
ACCESSION   DAOSWR010000126 DAOSWR010000000
VERSION     DAOSWR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9776_length_5538_cov_5.224695"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1018)
                     /locus_tag="PK513_08685"
     CDS             complement(176..1018)
                     /locus_tag="PK513_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82565.1"
                     /translation="MDIDISKLRERYEQACAIRANMKTLVDDLAAYPGMGCQKHSALE
                     AFSEGLLGKSVDDISIDTPQELQDLLPDISQEIWARQAETVLDDYAGILYARHYNHIL
                     FRKAMGEIVKDALLKSGLSAEFMDTQKAFDQNHSEEISRFREGLDILEREAVHNPMLF
                     EKIRAGRAEIAGDVLACDYTEDKDGLKTLITALGARSKISLAEIELYEASAQISFLYI
                     FERKCGFGLYNGRESRRKWNRDLMGMLKRVDEVLEKAGEEFGRLSVDCNSEIVRDDDS
                     FEFG"
     gene            1195..1416
                     /locus_tag="PK513_08690"
     CDS             1195..1416
                     /locus_tag="PK513_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82566.1"
                     /translation="MEIRSPLDKQSGITALFGALAAGAVLITALALSDIPAEEQGLTA
                     DYNNNSNNSFVFEIAEKDMTPPVPDYAQQ"
     gene            complement(1426..2706)
                     /gene="nuoF"
                     /locus_tag="PK513_08695"
     CDS             complement(1426..2706)
                     /gene="nuoF"
                     /locus_tag="PK513_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO82567.1"
                     /translation="MLNDEDRIFTNIYGWESFSLKQAQARGDWDNTKAILAKGRDWII
                     DEMKASGLRGRGGAGFPTGMKWSFMPKESDGRPHYLVVNADESEPGTCKDRDILRHEP
                     HKLLEGCLVAGFAMGAHAAYIYIRGEFQNEGSEVIKAIKEAYAAGLLGKNAAGSGWDF
                     DVVLHRGAGAYICGEETALIESIEGKKGQPRNKPPFPAMAGLYSCPTTVNNVETIAVA
                     PTILRRGGSWFSGLGRNEKNAGTKLFCISGHVNQPCNVEEVMGIPLKELIEKHCGGVR
                     GGWDNLLAVIPGGSSTPMLPKEMCENLHMDFDSLRELGTGLGTAGVIVMDKSTDVIKA
                     ICRLSQFYMHESCGQCTPCREGTGWVWRVMKRMVAGNASVEEIDMLFEVTKEIEGHTI
                     CALGDAAAWPVQGLIKHFRSEMEHRIMEYRKAAA"
     gene            complement(2707..3366)
                     /gene="nuoE"
                     /locus_tag="PK513_08700"
     CDS             complement(2707..3366)
                     /gene="nuoE"
                     /locus_tag="PK513_08700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOO82568.1"
                     /translation="MKKPFELHADYQPDSFEFNKAYQKRVDEAISLYPKGRQQSAVMP
                     LLDLAQRQVGEEGAKAKPPYGGWIPRAAMDKIAEILDMAPIKVYEVATFYSMYNTAPV
                     GKYLVQICGTTPCWLCGSDNIIKTCTKHLSIGMGETTKDGKFTLMEVECLGACVNAPM
                     VQINDDYYEDLTEGSMKEILDMLAEDMKPKIGSQKARKASMALSGPTTLKERAKKAGV
                     A"
     gene            complement(3367..4575)
                     /locus_tag="PK513_08705"
     CDS             complement(3367..4575)
                     /locus_tag="PK513_08705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOO82569.1"
                     /translation="MSAEKRDIDIVEETQIKPYTMNFGPQHPAAHGVLRLVLEMEGEI
                     VERADPHIGLLHRGTEKLIEHKTYTQAMPYFDRLDYVCPLNQEHAFVLATEKLLGISV
                     PKRGQYIRVLFAELSRVINHIFNITTFALDVGAVTPALWGFEEREKGMEFCERACGAR
                     LHANYFRTGGVSQDLPAGLLDDMLEWVEELPKFLTDLETLLTDNRVFKQRTVDIGIVS
                     KDQALNWGFVGPMLRASGVVWDLRKNQPYDSYEDFDFDIPVGKTGDCYARYLVRMAEI
                     YESLKIMKQAIEKMPDGPSRVNDYKVAPPPRGEMKSSMEAMIHHFKLFSEGFHVPAGE
                     TYTAVEAPKGEFGVYLVSDGTNRPYRCRIRAPGFAHLQALDFMSKGHQLADTVAIIGS
                     LDIVFGEIDR"
     gene            complement(4572..5234)
                     /locus_tag="PK513_08710"
     CDS             complement(4572..5234)
                     /locus_tag="PK513_08710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOO82570.1"
                     /translation="MATDALKDLADYIMENLDDSIASYDFFKGELSLNGNRENIVRIL
                     NFLRDDKECTFRVLVDICGADYPDRALGVGRFDVVYNLLSLRHNQRLRVKITTDEETP
                     VPSVVDIFSSAGWYEREVWDMYGVMFAGNPDLRRILSDYGFEGHPQRKDFPLTGYVEV
                     RYDDELKRVVYEPVKLSQDFRQFDALSPWEAMTDVQLSGDKKAAIPAHGWKASKEGRG
                     AG"
     gene            complement(5245..>5538)
                     /gene="nuoB"
                     /locus_tag="PK513_08715"
     CDS             complement(5245..>5538)
                     /gene="nuoB"
                     /locus_tag="PK513_08715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOO82571.1"
                     /translation="PRQSDVMIVAGTLTNKMAPALRRVYDQMPEPRWVVSMGSCANGG
                     GYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALVYGLLQLQKKIQREDTRIVR"
BASE COUNT         1509 a         1719 c         1015 g         1295 t
ORIGIN      
        1 aaacctccga cccctcacta cattgttgtt agcaagagcc cgccaagtcc gtttccgctc
       61 cgataacgcc tcaacgcggc tttctatcat atttcaccct cttttcacat aacttaagaa
      121 agaacatata aatatatttt ccagaagtca aaaacttcct caattttaaa aacctttagc
      181 caaattcaaa agaatcatca tcccgaacaa tctccgaatt acaatcaacg ctcaatctgc
      241 caaattcttc cccagccttt tccaaaacct cgtcaacacg ttttaacata cccatgagat
      301 cgcggttcca tttcctcctg gattcacgac cattatataa accaaaacca cactttctct
      361 caaatatata taaaaaagaa atttgcgccg aagcctcgta aagctctatc tccgcaagag
      421 aaatctttga acgtgcccca agagctgtaa tcagcgtttt aagaccatcc ttatcctccg
      481 tataatcaca cgcaagaacg tcaccggcta tctccgctct tcctgccctg attttttcaa
      541 aaagcatagg attatgtacc gcctcacgtt caagaatatc taacccctcc cgaaaacgcg
      601 aaatttcttc ggagtggttt tgatcaaatg ccttttgagt atccataaac tctgcagata
      661 gtccggattt taaaagcgca tctttaacaa tttctcccat cgcttttcgg aacaaaatat
      721 ggttataatg tctcgcatat aaaatccccg cataatcatc caaaactgtc tccgcctgac
      781 gcgcccaaat ttcttgagaa atatccggca acaagtcttg aagctcctgc ggagtatcaa
      841 tagaaatatc atcaacactc tttcccaaaa gcccttcact aaatgcttct aaagccgaat
      901 gcttttgaca gcccatccct ggataagccg caaggtcatc gaccaacgtc ttcatattgg
      961 cgcgaatagc acaggcctgc tcataacgct ctcttagctt tgaaatatca atatccataa
     1021 cgtttccgtt tcccaaggat cataatcttg gcaaccatag acaacaggca taattatgtc
     1081 aacaaatagc cgtgtatttt atagcacatt atacattttt attgacaaaa cacattacat
     1141 aacgtaaaaa ccactccaaa caactttatc gagcataata ttgggaacaa aaaaatggaa
     1201 ataagatcgc cattagataa acaaagtgga ataacggctc tgtttggcgc tctggccgct
     1261 ggagccgttc tgatcacagc cttagcgctt tcagacatac ccgcagaaga acaaggactg
     1321 accgctgact ataataataa cagcaataac agttttgtat ttgaaatcgc agaaaaagat
     1381 atgacaccgc ccgtaccaga ctatgcacaa caataagcca aaagcctaag ccgccgcctt
     1441 acggtactcc ataattctgt gctccatctc ggaacgaaaa tgcttgatca acccctgcac
     1501 cggccaggcc gccgcatcgc ccagcgcaca aatcgtgtgc ccttcaattt ccttggtcac
     1561 ctcaaacagc atatcaatct cttcaacgct ggcattgcct gccaccatcc gcttcatcac
     1621 gcgccagacc cagcccgtcc cttcacgaca cggcgtacat tgaccgcaac tctcatgcat
     1681 ataaaactgc gacaatcggc aaattgcctt gatcacatcc gtagacttat ccatcacaat
     1741 aacgcccgcc gtgccaaggc cagtaccgag ttcacgcaag gaatcaaaat ccatatgcaa
     1801 gttttcacac atctctttcg gcaacatcgg cgtcgaagac ccgcccggta tcacagccag
     1861 cagattatcc catccgccgc gcacaccgcc acaatgcttc tcaatcagct ctttgagcgg
     1921 aatgcccata acctcttcga cattgcatgg ctggttcaca tgccccgaaa tacaaaacag
     1981 cttcgtcccg gcattcttct cattccggcc cagcccggag aaccacgaac ctccacggcg
     2041 taagatcgtc ggcgccaccg caatcgtttc cacattattc accgttgtcg gacaggaata
     2101 cagccccgcc atcgccggaa acggcggctt attacgcggc tggcctttct tgccttcaat
     2161 cgattcaatc aacgcggttt cctcgccgca aatataagcc ccggccccgc gatgcaaaac
     2221 aacatcaaaa tcccaaccgg acccggcggc attcttcccc aacaatccag cagcataggc
     2281 ttctttaatc gccttaatca cttcagaacc ctcattctga aattccccgc gaatatagat
     2341 gtacgctgca tgcgccccca tcgcgaaacc ggcaactaaa cacccttcaa gcaatttatg
     2401 aggttcatgg cgtaaaatat cgcgatcttt acaagttccg ggctcagact catcggcatt
     2461 caccaccaga taatgcggcc gcccatccga ttctttaggc ataaaagacc atttcatccc
     2521 tgtaggaaaa cctgctccac cacgcccgcg caagccggag gccttcatct catcaataat
     2581 ccagtcccgc cccttggcca aaattgcctt agtattgtcc caatctccac gtgcctgcgc
     2641 ctgcttaaga gaaaaactct cccagccata aatattcgta aatattcggt cttcgtcgtt
     2701 aagcatttaa gccactcccg ccttttttgc ccgctcttta agagtcgtcg gtccggatag
     2761 cgccatcgag gctttacgtg ccttttgtga accaattttc ggcttcatat cctcggccag
     2821 catatcaaga atttccttca tgctcccctc ggtcagatcc tcgtaataat catcattgat
     2881 ctgcaccatc ggcgcattca cacacgcgcc aaggcattca acctccatca aggtaaattt
     2941 gccgtccttc gtggtctcgc ccataccgat actcaaatgc ttcgtacagg tcttgataat
     3001 attatcactc ccgcacagcc agcacggcgt cgtcccgcaa atctgcacca gatacttccc
     3061 cactggcgca gtgttataca tggaataaaa cgtcgccacc tcgtaaacct tgatcggcgc
     3121 catatcgagg atttctgcaa tcttatccat cgccgcgcgc ggaatccacc caccataggg
     3181 cggcttcgcc ttcgcgccct cttcacccac ctgacgctgc gccagatcca gcaaaggcat
     3241 caccgccgat tgctgcctgc ccttgggata cagcgagatc gcctcatcaa cacgcttctg
     3301 atacgcttta ttaaattcaa agctatccgg ctgataatca gcatgcaatt caaacggttt
     3361 tttcatctaa cgatcaatct ccccaaacac aatatcgagg ctgccgataa tcgccaccgt
     3421 atcggccagc tgatgcccct tgctcataaa atcaagcgcc tgcaaatgcg caaagcccgg
     3481 ggcgcgaatg cggcatctat aaggcctgtt cgtcccgtca ctcaccagat acacgccgaa
     3541 ctcgcctttg ggggcttcca cggctgtata ggtctcccct gccggcacat gaaagccctc
     3601 actaaacagc ttaaagtgat ggatcatcgc ctccatcgaa gatttcatct cgccgcgcgg
     3661 cggcggcgca accttgtaat cattgacgcg gctcggcccg tccggcatct tttcaatcgc
     3721 ctgtttcata attttcagag attcataaat ctcggccatc cgcaccagat agcgcgcata
     3781 acaatccccg gtcttcccaa ctggaatatc gaaatcaaaa tcttcatatg aatcataagg
     3841 ctggttcttg cgcaaatccc acaccacacc ggaagcgcgc agcatcggcc cgacaaagcc
     3901 ccaattcagc gcctggtcct tcgaaacaat accaatatcc accgtgcgct gcttgaaaac
     3961 gcggttatcc gtaagcaacg tttccagatc ggtcaggaat ttcggcaatt cttcaaccca
     4021 ctccagcata tcgtccagaa gccccgccgg aagatcctgc gatacaccgc cggtgcggaa
     4081 ataattcgca tgcaaccgcg ccccgcaggc ccgctcacaa aactccatgc ccttctcgcg
     4141 ttcttcgaac ccccatagcg ccggggtcac agctcccaca tccagagcaa aagttgtaat
     4201 attgaatatg tgattaatca cacggctcag ctcggcaaac agcactcgaa tatattgccc
     4261 ccgcttgggc acgctgatcc ccagcagctt ttccgtcgcc agaacaaacg catgctcctg
     4321 attcaaggga cacacataat ccagacgatc aaaatacggc atcgcctgcg tataggtctt
     4381 atgctcgatc agcttttccg tgccccgatg aagcagacca atatgcggat ccgcccgctc
     4441 gacaatctcg ccttccattt ccagaacaag ccgcaaaacc ccatgcgcag ccggatgctg
     4501 cggcccgaaa ttcattgtat agggcttgat ctgcgtctct tcgacaatat ctatatctcg
     4561 tttttctgcg ctcatcccgc accacgcccc tcttttgacg ccttccagcc atgcgcagga
     4621 atagccgcct tcttatcacc ggaaagctgc acatcggtca ttgcttccca cggcgataat
     4681 gcatcaaact ggcggaaatc ctgagacagt ttcacaggct cataaacaac gcgtttaagc
     4741 tcatcgtcat agcgaacttc aacataaccg gtcagcggaa aatccttgcg ctgcggatgt
     4801 ccctcaaaac cataatcgct caatatacgg cgcagatcag gattgcctgc aaacatcaca
     4861 ccatacatgt cccacacttc acgctcatac catcccgcag agctgaaaat atccaccaca
     4921 gacggcacag gcgtctcttc atctgttgta atttttacgc gtagccgctg attatgtcgc
     4981 aaggacagta agttatagac aacatcaaaa cggccaacgc ccaacgccct atccggataa
     5041 tccgccccgc aaatatcaac caaaacgcga aatgtacact ctttgtcatc ccgcagaaaa
     5101 ttcaaaatgc gcacaatatt ttcacgattg ccattcagcg aaagctcgcc tttaaaaaaa
     5161 tcataactcg caatggaatc gtccagattt tccatgatgt aatcagccag atccttaagt
     5221 gcatccgttg ccatcctcgc gcccctatct cacaatgcgc gtatcttcgc gctgaatttt
     5281 tttctgaagt tgtaacaacc cgtagacaag agcctctgcc gtcggcggac agcccggtac
     5341 atagatgtca actggcacaa cgcggtcaca tccacgcaca acggaatagg aataatgata
     5401 atacccgccg ccattagcac aactgcccat ggacacaacc cagcgcggct ccggcatctg
     5461 gtcataaacg cgccgcagcg ccggcgccat tttattggtc aaagtcccgg ccacaatcat
     5521 aacatcggac tgacgcgg
//
LOCUS       DAOSWR010000127         5485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_9916_length_5485_cov_4.781400, whole genome shotgun sequence.
ACCESSION   DAOSWR010000127 DAOSWR010000000
VERSION     DAOSWR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9916_length_5485_cov_4.781400"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK513_08720"
     CDS             <1..286
                     /locus_tag="PK513_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOO82572.1"
                     /translation="KAAILPLTAKEEHVPIAQKLYLELREEFPVDLDIKQNIGKRYAR
                     QDEIGTPYCFTIDNDTLEDQTVTVRERNTMAQERIHLDKVAEYLREKMKA"
     gene            322..783
                     /locus_tag="PK513_08725"
     CDS             322..783
                     /locus_tag="PK513_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82573.1"
                     /translation="MFLNLFKKCKWNSWAFAGAAFFIVILWFLTTYFTKSAPGADGSV
                     IYGFPLPFYRWGGFCGDGVCESFSSIFLLMDIVLLFTIPLIVSMLWAGFSGLVKKLLI
                     LTALGLMAAPWFTYMEAVLLASNIILVVFMMFAGIKILWLKRRKNSSKTKG"
     gene            complement(845..1714)
                     /locus_tag="PK513_08730"
     CDS             complement(845..1714)
                     /locus_tag="PK513_08730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOO82574.1"
                     /translation="MSVKTKVCETCDKLSNLPIIGGILKPKPRVSILRLSGVIADSGP
                     GRGGISHHKYEKFITDAFDEFDLQAVLLVINSPGGSPAQSALIGDQIRRLSEEKEVPV
                     YAFVEDVAASGGYWLAAAADEIYANETSIVGSIGVISAGFGFKDLINKYGVERRVHTS
                     GKNKSFLDPFTEEKPADLKRLKAIQGELHETFIRWVKERRGKKLKGKDEELFDGSFWS
                     AKTALELGLVDGFGEAKRFAKTKFGEEIKFVELGPERKLVSSLLGSGMKSSLAEDAIE
                     ALETKAVWSRYGL"
     gene            complement(1743..2465)
                     /locus_tag="PK513_08735"
     CDS             complement(1743..2465)
                     /locus_tag="PK513_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO82575.1"
                     /translation="MSNIIHILNKRIALTQHVGGFQTSIDAVLLAAACPAHAGERLLD
                     LGCGVGSASLAALKRIENTTLTGIDILPEVVELAKHNARLNAMVERTDFQCIDVRDFE
                     DLSFDHVICNPPFLDAGEHVRSPSQAKATAMGFGEDDMDLKDWVDCAHRSIAGQGSLT
                     LIHRADQIDEIIRAMGKRFGAVEIIPLWPKAGQEAKRVIVRARKHRKSPARIHAGIVL
                     HQENGDYTAHAENILREIAPIA"
     gene            complement(2570..3496)
                     /locus_tag="PK513_08740"
     CDS             complement(2570..3496)
                     /locus_tag="PK513_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOO82576.1"
                     /translation="MHELTNTIANNVLAAFSGQASKSVMNAIQSASAKTGVDFAYLMQ
                     QAKAESSFDPHAKARTSSATGLYQFIESTWLNTIEKHGYKHGISLEGKSRRQILNMRN
                     DPEIAANMAAEFASENQKFLEDHWAKGEKDIGATELYLAHFLGAGGAAGFLNARDENP
                     LQKAAYIFPEAAKANRNVFYEAGTSRPRTMDEIYAFFDKKFQIEGAPAPQAQPSAKST
                     PHSVVYQDIASEALLAFGVENSGFEYSRRSQDHLINRLFSRGHQELENIYGQHTASSP
                     YQALLHAPIEIMLLAELDLPTNPDITRHKSYL"
     gene            3597..5231
                     /locus_tag="PK513_08745"
     CDS             3597..5231
                     /locus_tag="PK513_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOO82577.1"
                     /translation="MSDYMPPIEDMQFLLHDVLGFENEDLDREMTESIFGEAAKLASE
                     VLAPLNLSGDQNGAKLGGTEVTTAPGWKEAYAGYRDGGWNAVPFDPEFGGQGLPYALA
                     FPVQEMWQGANTSFSLCPLLNQGAVEAINHHGSDEQKAAYLPKLISGEWTGTMNLTEA
                     AAGSDLGLIRTKAEPDGNAYKITGQKIFITYGEHDLAENIIHLVLARLPGAPDDIKGI
                     SLFIVPKFLEDGTRNAVKCIGLEHKLGIHASPTCTMDFDGATGYLVGQEHQGLKYMFT
                     MMNNARLSVGLQGVAIAERSYQQAKSFAAQRVQGKSFTTGERVTIDQHTDVQRMLLSM
                     KSQIEAGRALTYAAALALDNGETMRVDILTPLVKSWCTDMACEVTSIGVQIHGGMGFI
                     EETGAAQYYRDARILPIYEGTNGIQALDLTFRKTLRDNGESISAFIDDLRADAGDALS
                     PYFDMLEDATKALLDTGKQKDLDQVAAMATPYLNGFALIAAGVLLEKSNAALAERSDD
                     FAAEKRRTIAFYKGNILPMAKAHLHAAVHGAQCITN"
     gene            complement(5235..>5485)
                     /locus_tag="PK513_08750"
     CDS             complement(5235..>5485)
                     /locus_tag="PK513_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82578.1"
                     /translation="CRDAVGAAGRAYMECLSKANMFKPGTDPEEYCECVANTYVDMMN
                     TAAPRVSSRSIVRLQTYSYTACTNSQTGRPEVRFEDSR"
BASE COUNT         1334 a         1421 c         1344 g         1386 t
ORIGIN      
        1 aaaggctgcc atcctgccgc tgaccgcgaa ggaagagcac gtgcccatcg cgcaaaaact
       61 ctatctggaa ctgcgcgagg aattcccggt cgatctcgac atcaaacaaa atatcggcaa
      121 gcgctatgcc cgtcaggatg aaatcggcac gccctattgc ttcacgattg ataacgacac
      181 gcttgaggat caaaccgtca ccgtgcgtga gcgcaacacc atggcacaag agcgcatcca
      241 ccttgataag gtcgccgaat atctgcgcga gaagatgaaa gcgtagagtt ttaagacgca
      301 aagttagaaa ggttctatcg aatgtttctg aatcttttca aaaagtgcaa atggaatagc
      361 tgggcctttg caggggcagc gtttttcata gtcattttgt ggtttctaac gacatatttc
      421 actaagtcgg caccaggggc ggacgggtct gttatatatg ggtttccctt gccgttttat
      481 agatggggcg gtttttgcgg cgatggtgtc tgtgaatcat tctcctcaat attcctttta
      541 atggatattg tattgctgtt tacgattcct ttgattgtga gcatgctttg ggcgggattt
      601 tccggtttgg taaaaaaatt gctcatttta acagcgcttg gtttgatggc tgctccgtgg
      661 tttacataca tggaagctgt attactggca tcaaatataa tcttggtggt gtttatgatg
      721 tttgctggga tcaagattct ttggcttaaa agaaggaaaa actcttccaa aacaaaaggc
      781 taaaaagtct tcaaaacggg ccttgtttgt ctttgtctga ctttatgtcc tttgcattta
      841 aaaattacaa accataccgg ctccaaacgg ctttggtttc gagagcctca atcgcatctt
      901 ccgccagtga gcttttcata ccggaaccga gcaaggaaga caccagcttt ctctccggcc
      961 ccagctcaac gaatttaatt tcttcgccaa acttcgtttt tgcaaatctt ttcgcctcgc
     1021 caaacccgtc aaccagcccc agctccaacg ccgttttcgc actccagaaa ctcccgtcaa
     1081 acagctcttc atccttgcct ttaagctttt taccgcggcg ctccttgacc caccggataa
     1141 aagtttcgtg cagctctcct tgaatggctt ttaaccgttt tagatcggcg ggtttttcct
     1201 ccgtaaacgg gtccaaaaag cttttatttt taccggaggt atgaacgcgg cgctcaacac
     1261 catatttatt gattaaatcc ttaaaaccaa atccggcgga aatgacgccg attgatccga
     1321 caatcgaggt ttcgttcgca taaatttcat ccgccgccgc cgccagccag tatccaccgc
     1381 tcgctgccac atcttcgaca aaggcataaa ccggaacctc tttttcttcc gacaacctgc
     1441 ggatctgatc gccgatcagc gcactttgcg ccggagaccc gcccggtgag ttgatcacca
     1501 gcaacacggc ctgcaagtca aattcatcaa aagcatcggt gataaatttc tcatatttat
     1561 gatgactgat cccgccgcgc cccggcccgg aatcagcaat cacccccgat aagcgcaaaa
     1621 tagaaacgcg cggcttgggc ttgagaattc cgccaataat cggcagattc gagagtttat
     1681 cgcaggtttc acagactttc gttttcacgg acatatcaag ggttcctctt tgagcatgca
     1741 atttaagcta tgggggcgat ttcacgcaag atgttttccg cgtgcgccgt ataatcaccg
     1801 ttttcctgat gcaaaacgat cccggcgtga attctcgccg ggcttttgcg gtgcttacgc
     1861 gctcgcacaa tgacgcgctt ggcctcttgc cctgctttcg gccataacgg gataatctca
     1921 accgcgccaa agcgcttgcc catggcccgt atgatttcat caatctgatc ggcccggtgg
     1981 atcagtgtga gcgagccttg tccggcaatg ctgcgatggg cgcaatccac ccagtccttc
     2041 agatccatat cgtcctcgcc aaatcccata gccgtggctt tagcctgaga aggggagcga
     2101 acatgctctc ccgcatccag aaaaggcggg ttacagatca catgatcaaa acttagatct
     2161 tcaaaatcgc gaacatcaat gcactgaaaa tccgtgcgct ctaccatggc gttcagcctg
     2221 gcattatgct tagccaattc aacgacttct ggcaaaatat caattccggt cagtgttgtg
     2281 ttctcaatac gttttaacgc ggccaaagag gcgctgccca cgccgcagcc taaatccaga
     2341 aggcgttccc ccgcatgcgc cgggcaagca gcagccagca gcaccgcatc aattgaggtc
     2401 tgaaaaccgc ccacatgttg agtcagtgca atgcgcttat ttaaaatatg gataatattg
     2461 ctcatgaaga ctagctatag ccgtcattgc gaacgaagtg aagcaatcca gacaaaaaaa
     2521 tagattgccg cgcccatcct tcgggttgtc tcacaatgac gataaacgat tataaatacg
     2581 atttatgccg cgtaatatca gggttggttg gtaaatccag ctcggccagc agcataatct
     2641 caatcggcgc gtggagaaga gcctgataag gagaggaggc ggtgtgctgc ccgtagatat
     2701 tctcaagctc ttggtgtccg cgggaaaaca accggttgat caggtgatcc tgagagcgtc
     2761 tgctatattc aaaccctgaa ttttcaacgc caaaggccag caacgcctcg ctggcaatat
     2821 cctgatacac cacagagtgc ggcgtactct tagccgatgg ctgggcttgc ggagcagggg
     2881 cgccttcaat ttgaaacttt ttatcaaaga aggcgtaaat ctcatccatc gtacgcgggc
     2941 gcgaagtgcc agcttcataa aacacattgc ggttggcctt ggccgcttcc gggaaaatat
     3001 aggctgcttt ttgtagcgga ttttcatcac gcgcattcag gaagcccgcc gcgcctcccg
     3061 ctcccagaaa gtgcgccaga tacagctctg ttgcgccgat gtctttctcg cctttcgccc
     3121 agtgatcttc aagaaacttt tggttctcac tggcaaattc tgccgccata ttggccgcaa
     3181 tttccgggtc gttgcgcata ttcaggattt gccgacgaga cttgccctca aggctgattc
     3241 catgcttata gccatgtttt tcaatcgtat tcagccatgt gctctcaata aactgataca
     3301 gccccgtagc gctgctggtc ctggccttgg catgtgggtc aaagctgctc tcagccttgg
     3361 cttgctgcat cagataggca aaatcaaccc cggttttggc gctggcgctt tgaatcgcat
     3421 tcataacgct tttggaggct tggccgctaa aagccgctaa cacattgttt gcaattgtat
     3481 ttgttagttc gtgcatgagg tctgtatttc tcgttaaatt cttctacaat gtctcttcaa
     3541 aagtcttttc aaaagtagtc tgcaaccacc gtgccaaaat taaaagagag ccaaaaatga
     3601 gtgattatat gcccccgatc gaagatatgc agttcctgct tcatgatgtt ttagggtttg
     3661 aaaatgaaga tctggaccgg gaaatgacag aatctatctt tggcgaggcc gccaaactgg
     3721 ccagtgaagt gctggccccg ctaaatttga gcggcgatca aaacggcgca aagcttggcg
     3781 gcaccgaagt caccaccgca ccgggctgga aagaggctta tgccgggtat cgtgatggcg
     3841 gctggaatgc cgtgccgttt gatcctgaat ttggcgggca ggggctgcct tacgcgctgg
     3901 cttttcctgt tcaggaaatg tggcagggtg caaatacgag cttcagcctg tgtccgctgc
     3961 tcaatcaggg cgcggtcgag gcgattaatc atcatggctc ggatgaacaa aaggcggcct
     4021 atctacccaa actcatcagc ggtgagtgga ccggcacgat gaaccttacg gaggccgccg
     4081 ccggatctga tctcggtttg atccgtacca aagcggagcc tgacggcaac gcctataaaa
     4141 tcacgggtca gaaaatcttt attacctatg gtgagcatga tctggctgaa aatatcattc
     4201 atctcgttct tgcgcgcttg ccgggcgctc cggatgatat caaaggcatt tcattgttta
     4261 ttgttcctaa atttctggaa gatggcacac gtaacgctgt gaaatgtatc ggactggagc
     4321 acaagctcgg tatccacgcc tcgcccacct gcactatgga ttttgatggt gcgaccgggt
     4381 acttggtcgg gcaggagcat caaggcctca aatacatgtt cacaatgatg aataatgcgc
     4441 gcctgtctgt gggcttgcag ggcgtcgcga ttgcggaaag aagctatcaa caggccaaaa
     4501 gctttgccgc ccagcgcgtg cagggcaagt cattcaccac cggcgagcgc gttaccatcg
     4561 accagcatac ggatgtgcag cgcatgctgc tgagcatgaa atcacaaatt gaggcaggcc
     4621 gggcgttgac ctatgccgca gctctggctc ttgataatgg tgagaccatg cgcgtcgata
     4681 ttcttacgcc tctggttaaa agctggtgca cggatatggc ttgtgaagtc acgtcgatcg
     4741 gtgttcaaat ccatggcggc atgggcttta ttgaagaaac cggcgcagcg caatattacc
     4801 gcgatgcgcg cattttaccg atttacgaag gcacaaacgg cattcaggcg cttgatttga
     4861 cctttcgcaa aaccctgcgt gataatgggg aaagtatcag cgcctttatt gacgatctgc
     4921 gcgccgatgc aggtgatgcg ctaagcccat acttcgacat gctcgaagat gccaccaaag
     4981 cgcttctgga tacaggcaag caaaaagacc ttgatcaagt cgccgccatg gccacgccgt
     5041 atcttaatgg ctttgcgctt attgccgccg gggtgctatt ggaaaaatca aacgcggcgc
     5101 tggccgaacg ttcagatgac ttcgctgctg aaaagcgccg taccattgct ttttataaag
     5161 gtaacatcct gcccatggct aaggcgcatt tacacgctgc cgtgcatggc gcgcaatgca
     5221 taacaaatta aagactatcg actatcttca aatctaacct ctggtcgccc ggtttgtgaa
     5281 ttggtgcagg cggtataaga ataggtttga agccggacaa tgctgcggga actcacccgc
     5341 ggcgcagccg tattcatcat atccacataa gtattggcaa cgcattcgca gtattcttcc
     5401 gggtctgtac ccggtttgaa catattggcc ttgcttaggc attccatata agctcttccg
     5461 gccgcgccaa ccgcgtcgcg gcatt
//
LOCUS       DAOSWR010000128         5452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10006_length_5452_cov_5.199555, whole genome shotgun sequence.
ACCESSION   DAOSWR010000128 DAOSWR010000000
VERSION     DAOSWR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10006_length_5452_cov_5.199555"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..407)
                     /locus_tag="PK513_08755"
     CDS             complement(69..407)
                     /locus_tag="PK513_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82579.1"
                     /translation="MADLSKSFFSIADVYAQPLRIKPRALKAASAVWILVLSQYYSDN
                     TKESKKVGAKIVMDLNVKDDKKAHSTAAKYSEEFQDLLASVRKEGFDPKHLQNIEEFN
                     LKHPHSFRTN"
     gene            475..810
                     /locus_tag="PK513_08760"
     CDS             475..810
                     /locus_tag="PK513_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO82580.1"
                     /translation="MKKTKKTYSRYTLEALKLLSGQIKAARVESGIIAADLAARAGIS
                     RGLLYRIENADPSCSIGVVFEVASLLGLPLFNADLDDLKAKNKFIEDKLALLPAKVKA
                     KKIEVDDDF"
     gene            800..2062
                     /locus_tag="PK513_08765"
     CDS             800..2062
                     /locus_tag="PK513_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOO82581.1"
                     /translation="MTSSAPTKEGYVWIWLPGKVQPVVAGKLTLAGSKILFNYGQSYL
                     ERENAIPIDPKELPLKAGVQKNKSAEALHGCLRDAAPDAWGRRVILNKLLGRKGKNAD
                     TADLGEMAYLMESGSDRIGALDFQASPTDYVPRLSGNAPLETLLHSAELVEKGVPLSE
                     ELAQALHHGTSIGGARPKAMLEADDKKYIAKFSSSTDVYNVIKAEFMAMRLAKHCGLK
                     VADVRLETVSGKDVLLIERFDRQRSSAGWARKLMLSALTLLELDEMGARYASYQDLAE
                     IIRHRFADPQKTLEQLFARLVFNILCSNNDDHARNHAAFWDGKALKLTPAYDICPQLR
                     SGEEATQAMLIEGDNRSSRLATALAGARHFLLDEKKAREIIDNQIKTIHSKWDDVAQE
                     ASLTDIEYTLFKERLFLRPYAFEGYSES"
     gene            2067..2393
                     /locus_tag="PK513_08770"
     CDS             2067..2393
                     /locus_tag="PK513_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82582.1"
                     /translation="MNNNFIGTWLFTKIENYDLNPSDLGVIRFDAEGRGEFEFDVNYG
                     AMSCGHGQNIVHFGWEGNSECDPAHGTGWAELDENNLNEITGCIEFFQGDEFEFTAVK
                     HGTKNS"
     gene            2374..2898
                     /locus_tag="PK513_08775"
     CDS             2374..2898
                     /locus_tag="PK513_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOO82583.1"
                     /translation="MALKTLKFFLSKEKLQELAGEKVYARGVDYHAKDCVELLFYAPC
                     RAAAEVHGTHPYRVDFSVSENRNLEADCTCPAMHDWGFCKHAVATALLLLDNEPAKNA
                     PQPKQKPDNFAKTYPNIAGWIQDGWIEIGRDGESASMIRILDRGGLVWEGGTRHKSID
                     GMLAEAEEELKDWM"
     gene            complement(2918..3229)
                     /locus_tag="PK513_08780"
     CDS             complement(2918..3229)
                     /locus_tag="PK513_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82584.1"
                     /translation="MLLSSWQRIRAFIKKYESEKKPMFVKFRVHNILERAIEDGIELG
                     MNRAHKHTDTPDHDLIKQKIEDAIMEQLWEVIDMTESIKVETMSDFLNPRREDVKDKD
                     A"
     gene            complement(3695..4417)
                     /locus_tag="PK513_08785"
     CDS             complement(3695..4417)
                     /locus_tag="PK513_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HOO82585.1"
                     /translation="MTQSKTERFEMRLDQTMINQVDAWRSEQEDMPSRSEAVRRLIEA
                     GLDVQNSPVRIRDGEKLILAMLRDLYHHHKVKDGEIDPDFVMEAVWGGHYWSLDWQYQ
                     GLFHGHEDKRRTVREVVDILDMWDFLEGSFEKLTAKEKERFVKEMEPFTDVKFPGFDG
                     NNEAEHLGVLSFLTGKMDRFERFKKREANSHCPVLDSYRRMYAVFEPMRPTLTGGSMS
                     LSQIIDVLSARWPEDRRLKANQ"
     gene            4499..4759
                     /locus_tag="PK513_08790"
     CDS             4499..4759
                     /locus_tag="PK513_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82586.1"
                     /translation="MKLLKPKMLALLVPADREVVHHFGELEGFGLAAVQDGGGDVGRE
                     FGQADNFASFKARSSARSELIVKSVAINMFFIGLPPSFCTKS"
     gene            4899..5144
                     /locus_tag="PK513_08795"
     CDS             4899..5144
                     /locus_tag="PK513_08795"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOO82587.1"
                     /translation="MMKQNEHGQERESGVYYAPWEKSADRILTPFEHFIQRETASSLF
                     LIAATILALILANSIFSALYYDITHMKLGFTLGAIKL"
     gene            5179..>5452
                     /locus_tag="PK513_08800"
     CDS             5179..>5452
                     /locus_tag="PK513_08800"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOO82588.1"
                     /translation="MALFFFVVGLELKREILVGELASARKAALPIIAAIGGMALPALI
                     YYAVNPSGTAAQGWAIPMATDIAFAIGIMVFLGNRVPRALMAFLVAL"
BASE COUNT         1397 a         1245 c         1333 g         1477 t
ORIGIN      
        1 cttcggtcat agatcgtagc ttaactcact gtccgaattc ttgggaccac ctctgaaaac
       61 actaacaatt aatttgttct aaaactatga gggtgcttga ggttaaactc ttctatattt
      121 tgtagatgtt tcggatcaaa accttcttta cgaacacttg ccaaaagatc ttggaattct
      181 tcgctgtatt ttgctgctgt tgaatgtgct tttttgtcat ctttaacatt cagatccatt
      241 acaatcttag caccaacttt tttggattct tttgtattat ctgaataata ctgcgataat
      301 accaatatcc aaaccgcaga ggcggctttg agtgcgcggg gtttgatccg caagggttgg
      361 gcatagacgt cagcgatgga aaagaatgat tttgacagat ctgccaaact aacctataat
      421 gttttatata tagcgcatta aaatgtttaa tgctttaatt ataagacatt ataaatgaaa
      481 aaaacaaaaa agacatattc acgctatacg cttgaagccc tgaagctttt atcgggacag
      541 attaaggctg cgcgggtgga atcaggcata attgctgcgg atttggcggc gcgcgccggt
      601 atttcacggg ggttattata ccgcatcgaa aacgccgatc cgtcctgtag cattggggtg
      661 gtgtttgaag ttgccagttt gcttggattg ccgcttttta atgctgatct ggatgacctg
      721 aaggctaaaa acaaatttat cgaagataag ctggctttgc tccctgcaaa ggttaaggcc
      781 aagaaaatag aggtggatga tgacttctag cgcacccacc aaggaggggt atgtctggat
      841 ctggcttcct ggcaaggtac agcccgtggt cgctggaaaa cttacactgg ccggatcaaa
      901 gattttgttc aattacggtc agagttatct ggagcgtgaa aacgccattc cgattgatcc
      961 aaaagaatta cctttaaaag cgggggtaca gaaaaacaaa agcgcggagg ccttgcacgg
     1021 ctgtttacga gacgctgcgc cggatgcctg ggggcggcgt gtgattttga acaaactgct
     1081 cgggcgtaaa ggaaaaaatg ccgataccgc tgatttgggt gagatggcgt atctgatgga
     1141 atccggctca gaccggatag gtgcgctcga ttttcaggct tcgcctacgg attatgttcc
     1201 ccgattatca ggcaatgcac cgcttgaaac cttgctgcac tcagcagaac ttgttgaaaa
     1261 aggcgtgcct cttagtgaag aactggcgca ggctctccat cacggaacgt ccatcggcgg
     1321 cgcacgcccc aaagccatgc tggaagcaga tgataaaaaa tacatcgcta aattttcatc
     1381 gagcacggat gtttataatg tcatcaaggc cgaatttatg gcgatgcgcc tggcaaaaca
     1441 ttgcggtctg aaggtggctg atgttcggct ggagactgtg tcgggcaaag atgttttgtt
     1501 gatcgagcgt tttgatcggc aacgctcttc tgcgggttgg gcccgcaaat tgatgctctc
     1561 ggcgctcacg cttttggaat tggatgaaat gggtgcacgc tatgccagct atcaggatct
     1621 ggcggaaatt atccgtcacc gatttgcaga tccgcaaaaa acgctggagc agctttttgc
     1681 gcgtttggtg ttcaatattt tgtgcagcaa taacgatgac cacgcccgca accatgcggc
     1741 attctgggac gggaaggctc tgaaattaac cccggcctat gatatttgcc cacagcttag
     1801 aagcggtgag gaagcaacgc aagccatgct gatagagggt gacaaccgtt ccagccgctt
     1861 ggccacagcc ctggccggtg ctagacactt tttgctggat gaaaaaaagg ccagagaaat
     1921 tatcgacaac cagattaaga cgatccattc aaaatgggat gacgtggcgc aggaagcgtc
     1981 cttaacagat attgaatata cgcttttcaa agagcggctt ttcctaaggc cgtatgcttt
     2041 tgaagggtat tcggagagtt gaggcaatga acaataattt catcggcacg tggcttttta
     2101 caaaaataga aaattatgac ctcaatccat ccgatttagg cgtaatacga tttgatgcgg
     2161 agggacgtgg agagtttgaa ttcgatgtga actacggtgc tatgagctgt ggccatggac
     2221 aaaatattgt gcattttggt tgggagggaa atagcgaatg cgatcctgct cacggcacgg
     2281 gctgggcaga gcttgatgag aataatctca atgagataac aggttgcata gaattttttc
     2341 aaggtgatga atttgaattt acagcggtga aacatggcac taaaaactct taaatttttt
     2401 ctgtcgaaag aaaaactcca ggagctggcc ggtgagaaag tttatgcgcg cggagttgat
     2461 tatcatgcaa aagattgcgt agagcttttg ttctacgcac catgcagagc tgccgccgaa
     2521 gttcacggaa cacatcctta ccgcgtcgat tttagcgttt ccgaaaacag aaatttggaa
     2581 gctgattgca cctgtccggc catgcatgac tggggatttt gcaaacatgc cgtcgccacg
     2641 gctctgttac ttctggacaa cgagccggca aaaaacgctc ctcaaccaaa acaaaaacct
     2701 gataattttg caaaaaccta tcccaacatc gcgggctgga tacaggatgg ctggattgag
     2761 attggccgcg acggagagag cgcctcaatg atccgtattc tggatcgcgg tggcctggtc
     2821 tgggaaggtg gcacgcggca taaatccatt gatggcatgt tggctgaggc ggaagaagag
     2881 ttgaaagact ggatgtgatg tcttgcttga ttataaatta cgcgtcttta tcttttacat
     2941 cttctcggcg aggatttaaa aaatcagaca tagtttccac ttttatagat tcggtcatat
     3001 ctataacctc ccagagttgt tccatgatgg catcctcgat cttttgcttg attaggtcat
     3061 ggtcgggcgt gtctgtatgt ttgtgtgcgc gattcatgcc caactcgata ccatcttcga
     3121 tggcgcgctc taaaatatta tggactctaa atttcacaaa cataggtttt ttctcacttt
     3181 catatttctt aataaatgcg cggattcttt gccaagaaga caataacatg gaagcttatt
     3241 ttatcaagga aatgagagtg ttataaaacg ggataatgaa tgacaaacgc ggattcgaac
     3301 tggcgcattg acacctgaaa ttctctgtgc aatgatttaa caatcaaatc aaggcgttgc
     3361 agcggagaca aagttttaat ttgttcgggc tgcacagcca ttcatcactt tccaaactct
     3421 ttccagactt ttgcatcacc gccggaaggc ccagcgttcg tgggctttct ggccatgtta
     3481 ccgctcactt tccgccgccc tggccgcgta tatttttggc gttttcgtct atatacgggc
     3541 ctttttctct ccggaccgct accttcgctc ctggaaacag agcggtcgta aagcctttat
     3601 ttctgcggcc ttgcggatgg tgacataatc actggttctt agacggtgcg gataccaagg
     3661 gagatttaga gaaaaccgct aaagtagatt gctgctactg gttagctttc agccgtcgat
     3721 cttccggcca ccgagccgac aagacatcaa ttatttgtga gaggctcatg ctgccaccag
     3781 ttaatgtagg ccgcatcggt tcgaaaacag cgtacatgcg gcgataacta tctaggaccg
     3841 gacagtgaga attggcttcg cgttttttga aacgctcaaa tcgatccatc tttccagtaa
     3901 ggaaacttaa aactcccaga tgctcagctt cattatttcc atcgaagccg ggaaacttta
     3961 cgtcagtaaa aggctccatt tctttcacaa aacgctcttt ctcttttgcc gtaagctttt
     4021 caaagctccc ctccagaaag tcccacatat caagtatatc gacgacttcc cttacagtac
     4081 gccgcttatc ttcatgaccg tggaacagcc cctgatattg ccagtcaagg ctccaataat
     4141 ggccgcccca aacagcttcc atcacgaaat caggatcgat ttctccgtct tttactttgt
     4201 gatgatggta aaggtcgcgc agcatggcca gtataagctt ttccccatca cgaattctga
     4261 caggactatt ctgcacgtcc agccctgctt ctataaggcg tcttacagct tcagaacgcg
     4321 atggcatgtc ttcttgttcg ctgcgccatg catccacttg attgatcatg gtttgatcca
     4381 gacgcatttc aaagcgctct gttttgcttt gtgtcattta aaactctcct ctattacgta
     4441 gtaaaatcgt atatatgtac gtaatatacg gcataatcac ggcgattaca atagtaaaat
     4501 gaagttactg aaacccaaaa tgctggcgtt gctcgtccca gcggaccggg aagtcgtcca
     4561 tcatttcggc gagctggagg gctttgggct ggcggccgtt caggatggcg gtggtgatgt
     4621 cgggcgcgag ttcggtcagg cggataattt tgccagcttc aaggcgcgct ccagcgcccg
     4681 atctgagtta atcgtcaaat ccgttgctat caatatgttt ttcattggcc ttcctccatc
     4741 tttttgcacg aaatcgtaat attaagcata tcactttgcc agtaaatccg gatatcaaaa
     4801 caagcgcttt ccctttcgga gcgttgaccg cttttccttt taaaccacct ttatttgcgt
     4861 tataaaaaag caaagcaatt gcagtaaaaa ggcgcttgat gatgaagcag aacgagcacg
     4921 gacaggagcg tgaaagcggt gtttattacg cgccgtggga aaaatcggca gaccgcatcc
     4981 tgacgccgtt cgagcatttc attcagaggg aaacggcaag ctcgctgttc ctgatcgccg
     5041 caactatcct cgccctgatt ctggccaaca gcattttctc tgcgctttac tacgacatta
     5101 cccatatgaa actgggtttt acgctcggcg ctataaagct ttgaaaacac cctgcatcac
     5161 tgggtcaatg acggcttgat ggcattattc tttttcgtcg tcgggctgga actcaaacgt
     5221 gaaattcttg tcggcgaact ggccagcgcc cgcaaggcgg ccttgccgat catcgccgcc
     5281 atcggcggca tggcgcttcc cgcgctgatt tattacgcgg taaacccgtc cggaacggcg
     5341 gcgcaaggct gggcgattcc gatggcgacc gacatcgcct ttgccatcgg gatcatggtg
     5401 tttttgggaa acagggttcc gcgcgcgctg atggcgtttt tagtggcgct gg
//
LOCUS       DAOSWR010000129         5402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10128_length_5402_cov_4.770713, whole genome shotgun sequence.
ACCESSION   DAOSWR010000129 DAOSWR010000000
VERSION     DAOSWR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10128_length_5402_cov_4.770713"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..509)
                     /locus_tag="PK513_08805"
     CDS             complement(57..509)
                     /locus_tag="PK513_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82589.1"
                     /translation="MAGDLDKCLSLTAEYNESSLIASINRVMDMHVIDDPILLNAKEG
                     MYAVHDHVLIGDDIKLYTKQEFDEEFADKKPISYAQYVEDRNTISENRFHERMPAVAT
                     AGNGKLSGMNMRIEQIEKDAKEFGIELDTKSGVEVLPNPPSDDPACKF"
     gene            636..2036
                     /gene="leuC"
                     /locus_tag="PK513_08810"
     CDS             636..2036
                     /gene="leuC"
                     /locus_tag="PK513_08810"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOO82590.1"
                     /translation="MKPRTLFEKIWDDHVVDRQEDGTCLIYIDRHLIHEVTSPQAFEG
                     LRMAGRKVRRTENTLAVMDHNVPTTDRSQPIEQEDSRIQIETLRKNCAEFGVMLFDLH
                     DQRQGIVHIIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLI
                     QKPAKTMRITVDGALPDAVTAKDMILAIISKIGTAGGTGYVIEYAGEAVRALSMEGRM
                     TICNMSIEAGARAGLVAPDETTFAYIKGRPMAPKGEDFDKAVAYWKTLPSDEGATYDK
                     EVVLEASEIAPTVTWGTTPEDVVPITGCVPSPDDFADANKRASVQRMLEYMGLEPGTK
                     MTDIPVDKVFIGSCTNARIEDLREVARIAEGKKVSEGVEAMIVPGSGLVKAMAEEEGL
                     ADILIEAGFDWREPGCSMCLGMNPDQLQPGERCASTSNRNFEGRQGKGGRTHLLSPAM
                     AAAAAITGKLTDVREL"
     gene            complement(2031..2660)
                     /locus_tag="PK513_08815"
     CDS             complement(2031..2660)
                     /locus_tag="PK513_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82591.1"
                     /translation="MRDIKNKKKIITVSCFLAFFAGNVYAEEIAAYRTALPVFTISSA
                     QNAFQWNDPSYDYRLNTGAGERMPIEISAAETDTPLGADQTLSGNLAKSEEGEDYADK
                     ILAKIPYNKQLKSTWKLIDGDTDLYVKGLRVDRGNKGVEYSTSTMPFVGEVEGIKFKA
                     EAGEENKIKFESSRIPFIGNVEGFKFKSSVGEESSVSVRYTTSLEKLGL"
     gene            complement(2771..3073)
                     /locus_tag="PK513_08820"
     CDS             complement(2771..3073)
                     /locus_tag="PK513_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82592.1"
                     /translation="MKNSIGLLMLLAFLATMVKISAHAGEAQTYKAPENKADHGISYD
                     KEDKKLIVSFAGHDEEEGVLELFVAKDAMSANRPSPLASELNRISPAAGMQFRLEF"
     gene            3158..3859
                     /locus_tag="PK513_08825"
     CDS             3158..3859
                     /locus_tag="PK513_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO82593.1"
                     /translation="MENKWPDNFDLRDAPEIGNRFRGFIAEQLEALALYDQKRKDNAS
                     AEVIYIFHEHAQRVAKNVKRTCLHMGLSPLVAENMYWAVLPHDIGKRLLPVELWDQEE
                     KPSGRVKKVRRLHTLLGAQIVQEIFPDIEHPFKDLMMDIMRYHHEQMDGNGPHGVMGD
                     QLSAPVRLAAIVEAYDGWRIWRPHFGTRDISSPGVLKRMREEKGAEVFDMVLFEAFAK
                     MKMDDYKEGQILQDN"
     gene            3863..4468
                     /gene="leuD"
                     /locus_tag="PK513_08830"
     CDS             3863..4468
                     /gene="leuD"
                     /locus_tag="PK513_08830"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019954548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOO82594.1"
                     /translation="MKPFSTLKAVAAPLEMINVDTDIIIPKQFLKTVKRTGLGVNAFY
                     NIRYDDDGAPLPDFVLNKPEYTEAEILITGENFGCGSSREHAPWALLDMGFRCIVAPS
                     FADIFFNNSFKNGILPIELPQYQVDQLMGSAKNDPDGEIEIDLEKQTITRGNKFTFAF
                     DIDPFRKYCLLNGLDDIGLTLGKGTAIDNFEDKRTSKQPWM"
     gene            4476..5159
                     /locus_tag="PK513_08835"
     CDS             4476..5159
                     /locus_tag="PK513_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOO82595.1"
                     /translation="MIPPFKKKKPHKTREGKIALTLKAHAIDTVIDIGANNGQTRDYL
                     RFGGYEGDIISVEPLPALQRTLQNKAKRDPCWSVLPPLAIGDHDGTCLMNVSEASDMS
                     SALPSSSALMSALPKTRVVEQVEVPMKTLDTLLSDLNLEGRNVFVKMDTQGYEMRILE
                     NAPMALPALKGLQLELSLFELYEGETLYDDIIAFLKTRGFHAHMIVENNFSRKLNRQL
                     QVDGIFYKD"
     gene            5162..>5402
                     /locus_tag="PK513_08840"
     CDS             5162..>5402
                     /locus_tag="PK513_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO82596.1"
                     /translation="MTQKLVVLPGDGIGPEIVAEALKVLEWLREHAGLEVEVEHDLIG
                     GQAYDAYGTPFADETLKKCRSADAILMGSVGGPRWD"
BASE COUNT         1363 a         1250 c         1405 g         1384 t
ORIGIN      
        1 gaagcgctgg ctggccgata attcataatc gtcagattaa gttatgactt ttacaattaa
       61 aacttacacg caggatcatc tgatggaggg tttggcagca cctcaacgcc agatttagtg
      121 tctaattcta taccaaattc ttttgcatct ttttctattt gttcgatccg catattcatg
      181 cctgataatt ttccgttccc tgccgtagca actgcaggca ttctttcatg gaatctattt
      241 tcgctaatcg tattacgatc ttcgacatac tgcgcgtaac ttataggctt tttatctgca
      301 aattcttcgt caaattcttg cttggtatat aatttgatgt catcaccaat taaaacatga
      361 tcatgcactg catacattcc ctcttttgca ttgagcaaaa ttggatcatc aataacgtgc
      421 atatccataa cacgattaat tgaagcaatt agcgacgatt cattatattc cgctgtcaaa
      481 cttaaacact tatcaagatc accagccatg tttacacctt cttgttgttt attcctcacg
      541 tattaccttt attgacgggc ctgatgttct ttgtttaaat gcgcagcccg cgccacgtta
      601 aaattataaa tttaaggtct taaaatagag ttaaaatgaa gcctcgtacg ttgtttgaaa
      661 aaatctggga tgatcatgtc gtcgaccgcc aagaagacgg gacgtgtttg atctatatcg
      721 accggcatct gatccatgaa gtgaccagtc cgcaggcctt tgaaggcttg cgcatggccg
      781 ggcgcaaggt tcgccggact gaaaacacgc tggcggtgat ggatcataat gtgccaacca
      841 cggaccgcag ccagccgatt gagcaggaag acagccgcat ccagattgaa acgctgcgca
      901 agaactgcgc cgaattcggc gtgatgctct ttgatttgca tgaccagcgc caaggtatcg
      961 tccacattat cggcccggag caaggtttta ctctgcccgg catgacgatt gtttgcggcg
     1021 attcccatac ttcaacgcat ggcgcttttg gtgcgctggc cttcggtatt ggcaccagcg
     1081 aggtcgaaca cgtactggcg acgcaaacgc tgatccagaa acccgccaaa acgatgcgca
     1141 ttaccgttga tggcgccctg cccgatgccg tcacggccaa ggatatgatt ttggcgatta
     1201 taagcaagat tggcacggcg ggcgggacgg gctatgtgat tgaatatgcg ggtgaagctg
     1261 tgcgcgccct ttccatggaa gggcggatga caatctgcaa catgtcgatt gaagccggtg
     1321 cgcgcgccgg gctggtggca ccggatgaga caacatttgc ctatatcaag ggccggccga
     1381 tggcgccgaa gggcgaggat ttcgacaagg ctgtagccta ctggaaaacc ctgcccagtg
     1441 atgaaggggc gacctacgat aaagaagtgg tgctggaggc ctctgaaata gcgccgaccg
     1501 tgacctgggg gactacgcct gaggatgtgg tgcccattac cggatgtgtg ccctccccgg
     1561 atgattttgc ggatgccaat aaacgcgcgt cagtccagcg gatgctcgag tatatgggcc
     1621 ttgaaccggg tacaaagatg actgatatcc cggtggacaa ggtgtttatc gggtcgtgca
     1681 ccaatgcgcg aattgaggat ttgcgcgaag ttgcacgtat tgcagagggc aaaaaggtct
     1741 ccgagggcgt cgaggcgatg attgtccccg gctccggact tgtcaaagcg atggcggaag
     1801 aagaaggtct tgccgatatt ctgattgaag ccggatttga ctggcgcgag ccgggctgtt
     1861 cgatgtgtct ggggatgaac ccggatcaat tacagccggg agagcgctgc gcctcgacat
     1921 ccaatcgcaa ctttgaaggg cgtcagggca aaggcgggcg gacacacctg ctttccccgg
     1981 ccatggccgc agcggcggcg attaccggca agctcacgga tgtgcgggag ttataggccg
     2041 agtttttcca aagatgtggt gtagcgtaca gaaacgctgg actcctcgcc aacgctggac
     2101 ttgaatttga agccttcgac attgcctatg aagggaattc tggagctttc gaatttgatc
     2161 ttattctctt ccccagcttc ggccttaaat tttatgcctt caacttcacc aacaaagggc
     2221 atggtgctgg ttgaatattc aacgccttta ttgccgcgat caacgcgcag ccctttaaca
     2281 tacaaatccg tatcgccgtc aatgagtttc caggttgatt taagctgctt gttataagga
     2341 attttggcaa gaattttatc ggcgtagtcc tcgccctctt cgcttttcgc taaatttccg
     2401 cttaacgttt ggtcagcgcc tagcggcgtg tctgtttcag cggcgcttat ttctattggc
     2461 atgcgctctc ccgcgcctgt atttaagcga taatcatagg atgggtcgtt ccattgaaat
     2521 gcattttgag ccgaagatat ggtgaaaaca ggtaacgctg tgcgataagc cgcaatctct
     2581 tcggcataca catttccagc gaaaaacgcc agaaaacaag atacagttat gatcttcttt
     2641 ttatttttta tgtcacgcac ataattatta taacaaataa aatatatgcc caccaatttc
     2701 atatgcgctg caacgcaata tcagcgcggg attcagaagc ccttactccc ggaacaactg
     2761 tctattgacg ttaaaattcc aaacggaact gcatgcccgc ggcgggggag atgcggttaa
     2821 gttcactggc aagcggcgat ggcctgtttg cagacatcgc gtctttggct acaaacagct
     2881 ccagaacgcc ctcttcttca tcatggccag cgaacgacac aatcaacttc ttgtcttctt
     2941 tgtcatagga gatgccgtga tccgctttgt tctcaggcgc cttataagtc tgcgcttctc
     3001 ctgcatgcgc cgagatcttg accatagtcg ccaagaaagc cagcagcatt agcaatccaa
     3061 tcgaattttt catttgtttt gccctctttt tatggttttg ttacgcagcg atgacaaata
     3121 gcgtatcgca ataagcataa aattttacta aaacactatg gaaaataaat ggcctgataa
     3181 ttttgacttg cgtgatgcgc ctgaaatcgg taaccggttt cggggcttta ttgctgaaca
     3241 gcttgaggcg ctggcacttt atgatcaaaa gcgcaaggac aatgcctctg cggaagtgat
     3301 ctatattttc catgaacatg cacagcgggt ggcgaagaat gtaaagcgca catgcctgca
     3361 tatggggcta agtcctcttg tagcggagaa catgtattgg gctgttttgc cgcatgatat
     3421 tggcaagcgt ttgctgccgg ttgagctttg ggaccaggaa gaaaaaccaa gcggacgtgt
     3481 aaaaaaagtg cgccgcttac atacgctgct gggcgcacaa atcgttcagg aaatctttcc
     3541 tgatatagaa catcctttta aggacctgat gatggatatt atgcgttatc accatgagca
     3601 gatggatggg aatggtcccc atggtgtaat gggcgaccag ctctcagcgc cggtgcggct
     3661 ggcggcgatt gtcgaagcgt atgacggctg gcggatctgg cggccacatt ttgggacccg
     3721 cgatatttct tcgccaggcg tcttaaaacg catgcgcgag gaaaaaggcg cggaagtttt
     3781 tgatatggtg ttgtttgaag cctttgccaa aatgaaaatg gacgattata aagaaggcca
     3841 aatcttacag gataattaaa ttatgaagcc atttagcaca cttaaggccg tggccgcgcc
     3901 gctggagatg attaatgtcg ataccgacat tattatcccg aaacaatttc ttaagaccgt
     3961 gaaacgaacg ggtctgggcg tgaacgcgtt ttataacatt cgttatgacg atgatggggc
     4021 tcccctgccc gactttgtac tgaacaaacc ggaatatacc gaggcagaga ttttgatcac
     4081 gggtgagaat ttcggctgcg gctcgtcgcg tgagcatgcg ccatgggcac tgctggatat
     4141 gggatttcgt tgcatcgtcg caccgtcttt tgccgatatt ttctttaata acagctttaa
     4201 aaacggcatt ttgccgattg aattgccaca atatcaggtg gaccagttga tgggcagcgc
     4261 caagaacgac cccgatggtg aaattgaaat agatttagaa aaacagacta ttacgcgcgg
     4321 aaacaagttt acattcgcat ttgatattga cccgtttcgg aaatattgcc ttttgaacgg
     4381 gctggatgat attggcctga cgttgggaaa aggcacggcg attgataatt ttgaagacaa
     4441 gcgcaccagc aaacagccct ggatgtagcc gagcgatgat cccgcctttc aaaaagaaaa
     4501 aaccgcataa aacccgcgag ggaaaaatcg cgctgacgct aaaagcgcat gccattgata
     4561 cggtcattga tattggtgca aataacggtc aaacgcggga ttacttacgg tttggcggat
     4621 atgagggaga tattatctct gtcgagccgc tgcctgcctt acaaagaacg ctccaaaaca
     4681 aggccaagcg tgacccatgc tggagcgttt tgccaccact ggcgattgga gatcacgacg
     4741 gaacatgcct tatgaatgtg tccgaagcga gcgatatgag cagcgccctg ccctcctcca
     4801 gcgcgcttat gagcgctttg ccgaaaaccc gcgtggtgga gcaagttgaa gtgccgatga
     4861 aaacgcttga tacgcttctg tctgatttga atttggaagg caggaatgtg ttcgtgaaaa
     4921 tggatacgca aggctatgaa atgcgcattt tggagaatgc gccgatggct ctgcccgccc
     4981 ttaaagggct acagcttgag ctttcactgt ttgaacttta tgaaggcgaa acgctttatg
     5041 acgacattat cgcgtttttg aaaacgcgcg ggtttcacgc ccatatgatt gttgagaata
     5101 acttttcacg caaattaaac cgccagcttc aggtggatgg tattttttat aaggattaaa
     5161 catgacccaa aaacttgtcg tacttcccgg tgacgggatt ggcccggaga ttgtggcgga
     5221 agcgctgaaa gtgcttgaat ggctgcgcga gcatgccggg ctggaggttg aggtcgagca
     5281 tgatttgatc ggcgggcagg cctatgacgc ctatggcaca ccgtttgccg atgaaacgct
     5341 gaaaaaatgc cggagcgcgg acgcgatttt gatgggcagc gttggcgggc cgaggtggga
     5401 cg
//
LOCUS       DAOSWR010000130         5378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10192_length_5378_cov_4.970130, whole genome shotgun sequence.
ACCESSION   DAOSWR010000130 DAOSWR010000000
VERSION     DAOSWR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_5378_cov_4.970130"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1557
                     /gene="purF"
                     /locus_tag="PK513_08845"
     CDS             67..1557
                     /gene="purF"
                     /locus_tag="PK513_08845"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO82597.1"
                     /translation="MIADMPVFDDDKFHEECGVFGVFGSEDAAALTALGLHALQHRGQ
                     EACGVVSYDGQNYYSHKALGLVDSNFSDVSVIEKLKGHAAIGHNRYSTSGEPALRNVQ
                     PMYADMDFGGFSVAHNGNLTNAHMLRKELVKRGSIFQSTSDTETIVHLVAVSRKKSVA
                     DRLVDAVKQIKGAYSLVACAQSQIIGVRDPYGIRPLVLGRLGDAYILASETCALDIIG
                     ARFERDIESGEMVILDESGLKSLFPFKKQPKRFCIFEYIYFARPDSSMEGHSVYEMRK
                     NIGAELARENPVEGADLIVPVPDSGVPAAIGYAEASGVPFELGIIRNHYVGRTFIEPT
                     DKIRHLGVRLKHNANRKFIEGKSVVLVDDSIVRGTTSKKIVEMIKDAGAREVHMRISS
                     PPTIKSCFYGIDTPSSSELLAGQKSVEEIREYIKADSLAYISLDGLYKAVGETKRNAK
                     APQYCDACFTGDYPVELVDHDRNCADEKVTDLQERRVKPLKKLSKI"
     gene            complement(1547..2503)
                     /locus_tag="PK513_08850"
     CDS             complement(1547..2503)
                     /locus_tag="PK513_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO82598.1"
                     /translation="MTQELASQGSKQDSKRCVYDTELLARIEDLIRGFQAQVRDAQGS
                     ACEGAMVYGLRVLVALANMVLQKRDMFEIEPWHIISAHAPLSMPGEMLLRVKDFKNKN
                     MPPYEAIMAFYDHGYTAQIDGILFLAALGQFQRMRQTGREKQVSINISARSLRDSDFV
                     KVTLARLESLDLVSDEKVLIEIHESAPHLSLSRQVLELYRAVGVGFVIDDVGMNMNDV
                     LRLADFEGLAEFVKLDRHTVCAPEGEAYALDQVMSFVETLMPGTLCVAEGVQTAEHAW
                     DIVQKYPSILYAQGLYLPDEREVFQREFKAAQSDQGLASARS"
     gene            complement(2519..4204)
                     /locus_tag="PK513_08855"
     CDS             complement(2519..4204)
                     /locus_tag="PK513_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOO82599.1"
                     /translation="MPVQLIQHLKRRASQMAYNSVFYNWSLQGEAPERMVVRPVDPWP
                     GSMEAGKALCEGALMFEGAQMPFGAGCWQMDDLPEGWMAHLHGFSWLRDLRSLSGERG
                     MGAVARTHAKMMIRSWIEMNETWQPQSWRMDVAGQRLAMWISAYEFFSAVEFIEPEDE
                     EDFQNAFFDSCTRQARHLSRAIAHGEVDECRGPGRFYAVQGLLYAGIAFEGFEHWADQ
                     ALEQLAFELDAQIAGDGAHRSRSPAQLLEILQILLDIRMVLRAADRPLPEKVQHAIDR
                     MGPALRFFRYSDKHLALFQGAQEGAAERIDSVLQQAGARGKILSALPCTGYERLSLGR
                     TCVMMDCAGAPDWPYADSAHASPLSFEMSYGRARLFVNCGAHPLDEDWREALCSTPAH
                     TALSLDARNACGLQGRCGRGSFAPAVVLREDLRHAALLEASHNGYVPVNGFHHKRRIY
                     LSDQGHDVRGEDVLSADVLPVRPVDVAVRFHLHPKVMVSLIRDGQEALLRLPGGVGWR
                     FHQGSDLRNGRLELEDSVYLGCGTSIRKSKQLVIYGQIIDNSSKIKWAVQREG"
     gene            complement(4270..4929)
                     /gene="rpe"
                     /locus_tag="PK513_08860"
     CDS             complement(4270..4929)
                     /gene="rpe"
                     /locus_tag="PK513_08860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOO82600.1"
                     /translation="MPQKSVRIAPSILSADFSKLGEEVVAIDKAGADYIHIDVMDGHF
                     VPNLTFGAPVVKAIRGVTKKPFDVHLMISPVDPLIEDFVEAGADIITAHVEAGPHLHR
                     TLQAIKAAGVRCGVSLNPHTPIEAVQHVMNMVDLILIMTVNPGFGGQSFIPLLDKIAA
                     TRAMIEKSGRKIDLQVDGGVNSQTAKECIAAGADVLVAGTAVFKDGPESYAKNIEALR
                     G"
     gene            5186..>5378
                     /locus_tag="PK513_08865"
     CDS             5186..>5378
                     /locus_tag="PK513_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82601.1"
                     /translation="MLDPDEIEHLYKKAEEQSECARIRKQKEQTEQKARQKYHDGLSP
                     DELARRLRSRCNLNGLETLK"
BASE COUNT         1497 a         1502 c         1184 g         1195 t
ORIGIN      
        1 gaaatgcgtc agcagatgga ccagctgttt gatgaaaaaa cagatggttt gaatgagtga
       61 gatatgatga tagcggatat gccagttttt gatgatgaca agttccatga ggaatgcggc
      121 gtgtttggcg tgtttggtag cgaggatgcg gctgctttaa cggctcttgg tttgcatgct
      181 ttgcagcacc gggggcagga agcgtgcgga gtcgtatctt atgacggaca aaattattac
      241 tcgcataaag ctttggggct ggtggattcg aatttcagtg atgtgagcgt tattgaaaag
      301 ctgaaagggc atgccgcaat cgggcataat cgttattcaa caagcggtga accggctttg
      361 cgtaacgtac agccgatgta tgcggatatg gattttggcg gttttagcgt ggcgcataac
      421 gggaatttaa ccaatgctca tatgctacgc aaagagctgg tcaagcgcgg atctattttt
      481 caatcgacga gtgatacgga gacgattgtg catttggtgg ccgtgtcacg aaagaagtct
      541 gtggcggatc gtctggtcga tgcggtgaaa cagattaagg gggcgtattc gctggtggcg
      601 tgtgcgcaaa gccagatcat aggtgtacgt gatccgtatg gtattcgacc attggttttg
      661 ggacggctgg gtgatgctta tattctggcg tctgagactt gcgctttgga tattattggg
      721 gcgcgttttg agcgcgatat tgaatccggt gaaatggtga ttttggatga aagcggtttg
      781 aaatctctgt ttccatttaa aaagcaacct aaacgttttt gtattttcga gtatatttat
      841 tttgcgcgac ctgacagctc tatggaagga catagtgttt atgaaatgcg taaaaatatt
      901 ggcgcggagc tggcgcggga aaatccggtt gaaggcgctg atttgattgt acctgttccc
      961 gatagcggcg taccggcggc gattgggtat gcggaggcga gtggtgtgcc gttcgagctt
     1021 gggattattc gtaatcatta tgtcgggcgt acatttattg agccgaccga taaaatccgg
     1081 catttgggcg tacgtctgaa gcataatgca aaccggaaat tcattgaggg taaaagcgtt
     1141 gtgctggttg atgattctat tgtacgcggg acgacgagta agaaaattgt cgagatgatt
     1201 aaagatgccg gggcgcgaga agtgcatatg cggatttctt cgccgccgac gatcaaaagc
     1261 tgtttttacg ggattgatac accgtcatcc agcgagcttt tagccgggca gaaatcggtt
     1321 gaggaaattc gtgaatatat caaggctgat tcgctggctt atatttcact ggacggactt
     1381 tataaagccg ttggggaaac caagcgtaat gccaaggcgc cgcaatattg cgatgcgtgt
     1441 tttaccggtg attatccggt tgaactggtg gatcatgata gaaactgtgc cgatgaaaag
     1501 gtgactgacc tgcaagagcg ccgggtgaag cccctaaaaa aattatctaa gatctagcag
     1561 atgcaagccc ctgatccgac tgcgccgcct taaattcacg ctgaaacacc tcacgttcat
     1621 cgggcagata taatccttgc gcataaagaa tcgatggata tttttgtaca atatcccatg
     1681 catgttccgc tgtttgtacc ccttccgcga cacataacgt accgggcatc aaagtctcca
     1741 caaacgacat cacctgatca agcgcataag cctcaccctc tggtgcacac accgtatgtc
     1801 gatcaagttt tacaaactcc gccaacccct caaaatccgc cagacgcaac acatcattca
     1861 tattcatccc aacatcatca atcacaaacc cgaccccgac cgcccgataa agctccagaa
     1921 cctgacggct taaacttaaa tgcggcgcac tttcatgaat ctctatcaaa actttctcat
     1981 cgctgaccag atccagactt tcaagccgcg ctaaggtcac ctttacaaaa tcagaatcac
     2041 gcaaagaccg cgctgagata ttaatactca cctgcttttc acgcccggtt tggcgcatac
     2101 gctgaaattg accaagagca gccaaaaata aaatcccgtc aatctgcgcc gtataaccat
     2161 gatcgtaaaa cgccataatc gcctcataag gcggcatatt cttgttctta aaatccttca
     2221 cccgcagcag catctcgcca ggcattgaaa gcggggcatg cgcagaaata atatgccagg
     2281 gctcaatctc aaacatatca cgtttctgca aaaccatatt ggccagagcg accaacacac
     2341 gcaaaccgta gaccattgcc ccttcacatg ccgaaccttg cgcatcacga acctgtgctt
     2401 gaaaaccgcg aataagatcc tctatacgcg ccaaaagctc cgtatcataa acgcaacgtt
     2461 tagagtcttg cttagagcct tgagaggcca attcttgcgt catatacgca ggcctccctt
     2521 aaccttcgcg ttgcacagcc cactttattt tcgatgaatt atcaataatt tgaccataaa
     2581 taaccaattg tttgctctta cggatagaag tcccgcaccc caaatacaca ctatcttcca
     2641 gctccaaccg cccattgcgc aaatccgatc cctgatgaaa gcgccagcca accccgccag
     2701 gcaaccgcaa gagcgcctct tgcccatcgc gaatcagcga taccatcacc ttaggatgca
     2761 aatgaaagcg cactgccaca tccacaggcc gtaccggcaa cacatccgcg ctgagcacat
     2821 cctcgccgcg cacatcatgc ccctgatcag aaagataaat ccggcgctta tgatgaaacc
     2881 cattcaccgg aacatacccg ttatgagaag cctccagcaa cgccgcatgc ctcaaatcct
     2941 cacgtaaaac cacagcaggc gcaaaactgc cacgcccgca gcgcccctgc aaaccacaag
     3001 cattgcgcgc atcaaggcta agggccgtat gcgcaggcgt tgaacacagc gcttcacgcc
     3061 aatcttcatc cagcgggtga gcgccgcaat tcacaaacaa ccgcgcccgt ccgtaactca
     3121 tctcaaagct gagcggtgag gcatgcgcac tatctgcata cggccaatcc ggcgcgcccg
     3181 cacaatccat catcacacac gtacgcccca gactaagacg ctcatatccc gtgcacggca
     3241 aggcgctcaa aatctttcca cgtgccccgg cttgctgcaa aaccgaatcg atccgctccg
     3301 cggcgccttc ttgcgcaccc tgaaacaacg ccaaatgctt gtcgctataa cggaaaaacc
     3361 gtaaagccgg ccccatccgg tcaatagcat gctgaacttt ttccggcaaa ggccgatcag
     3421 cagcgcgcag caccatacgt atatcgagca aaatctgcaa aatctctaag agctgcgccg
     3481 gagagcgcga acgatgcgcc ccgtccccgg caatctgcgc atcaagctca aacgcaagct
     3541 gctcaagagc ctgatcagcc caatgctcaa aaccttcaaa cgcaatcccc gcatacagca
     3601 atccctgaac tgcataaaac cgcccgggcc cccgacactc atcgacctca ccatgcgcga
     3661 tggcgcgtga taaatgccgc gcctgacggg tgcagctatc aaaaaacgca ttctgaaaat
     3721 cttcctcatc ctcaggctca ataaactcaa ccgcactaaa aaattcatac gccgaaatcc
     3781 acatcgccaa tcgctgaccg gccacatcca tccgccagct ctgcggctgc cacgtctcat
     3841 tcatttcaat ccagctgcgg atcatcatct tggcatgcgt acgtgccacc gcgcccattc
     3901 cgcgctcccc gcttaaagac cgcaaatccc tgagccatga aaatccatgc aaatgcgcca
     3961 tccacccttc cggcaaatca tccatctgcc aacaccctgc accaaatggc atctgcgcac
     4021 cttcaaacat caacgcgccc tcacacaaag ccttgcccgc ctccatagag cccggccacg
     4081 ggtctaccgg acgcaccacc attcgctccg gcgcttcgcc ctgcaggctc cagttataaa
     4141 acacgctatt ataggccatc tgactggcgc ggcgtttcaa atgctgtata agctgaacag
     4201 gcatcgatcc tcttaaaacc ttgtaaattc ataaaaaacg cacaacgacc gccgcacgtt
     4261 caggtaaaat cagccccgca gagcttcgat attcttcgca taactttccg ggccatcctt
     4321 aaacacagcc gtcccggcca ccagaacatc cgccccggca gcaatacatt ctttggccgt
     4381 ctgcgaatta actccgccat caacctgtaa atctattttc cgcccagatt tttcaatcat
     4441 tgcgcgcgta gcggcaatct tgtccagcaa cggaataaaa ctttgcccgc caaaaccggg
     4501 gttcacagtc ataatcaaaa tcaaatccac catattcatc acatgttgga cagcctcaat
     4561 cggcgtatgc ggattaagcg aaactccgca cctgacgcct gcggccttaa tcgcctgcaa
     4621 cgtccggtgc aaatgcggcc ccgcctcaac atgcgcagta ataatatcgg ctcctgcctc
     4681 aacaaaatct tcaataagcg gatcaaccgg agaaatcatc aaatgtacat caaacggctt
     4741 cttcgtaact ccgcgaatgg ctttgaccac tggcgcacca aatgtcaggt tcggcacaaa
     4801 atgcccgtcc ataacgtcaa tatggatgta atcggcgccc gccttatcaa tcgcaaccac
     4861 ctcctcaccc aatttcgaaa aatcggctga caaaatcgac ggagcaatgc gaacagattt
     4921 ttggggcatt tcaaaactct taacaaccgt ggatagaact gtgaataaag cacctaagcg
     4981 cttcacttat aatagtttta acagagtttt atatgcaaat ctagaaatga gtgaaccatc
     5041 gtcgaattta tctatattta cgaaccaaaa aacacctccg tcattgcgag aagccctgcg
     5101 acgaagcaat ccataacacc acacctctac atctctatga ttaaaaaccc ttgacaaaat
     5161 aggaataata tcctatatat tgcacatgct tgaccccgat gaaatagaac atctatacaa
     5221 aaaagccgaa gaacagagcg aatgcgcacg aattcgcaag caaaaagaac agacggaaca
     5281 aaaagcgcga caaaaatatc atgacggcct atccccggac gaactggccc gccgtctccg
     5341 ctccagatgc aacctcaacg ggctagaaac cttgaaag
//
LOCUS       DAOSWR010000131         5365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10222_length_5365_cov_5.118832, whole genome shotgun sequence.
ACCESSION   DAOSWR010000131 DAOSWR010000000
VERSION     DAOSWR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10222_length_5365_cov_5.118832"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      164..257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            254..1318
                     /locus_tag="PK513_08870"
     CDS             254..1318
                     /locus_tag="PK513_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO82602.1"
                     /translation="MSQQPQNIAIIGAGITGLCSAYTMLEDGHGVTLYDPAGFPADNA
                     SFMAGGMLAPLAEIEHMPHEWIEAGLGSIALWAKFAEKHGDGLGFDFRQNGSLLLAHE
                     EDRYILQRFAAHLPAGKGEYTATSELEPALAEKFPQGLYLAEEAHINPAPAMHALCDY
                     LKAAGVTFIQEKADPTAISAQYDHVLDCRGMGAAWEDKNLRGVKGEIVIVHNTELALN
                     RPVRLMHPCYPLYIVPRPDHIFMIGATIIESSDNTQVSIRSGLELLSALYSLHPSFSE
                     AEIIEVKAGIRPSYPDNLPRIEISKNDNIIRCNGLFRHGFMLSPIIGRCVADHIAGGQ
                     NNFLNLLKKDRDEHHHQRAA"
     gene            1290..1490
                     /gene="thiS"
                     /locus_tag="PK513_08875"
     CDS             1290..1490
                     /gene="thiS"
                     /locus_tag="PK513_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOO82603.1"
                     /translation="MNIIINGRPKNFTAPLNLYDVLAQEEVIGMMIAVARNGTFVPKG
                     DYAHTILKDGDDIEIVAPMQGG"
     gene            1493..1948
                     /locus_tag="PK513_08880"
     CDS             1493..1948
                     /locus_tag="PK513_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82604.1"
                     /translation="MKKIFLLLAILLSVSACLKETRIDASSEEKLQKSIAEIMKELEP
                     DQQKNFSQSLKIIMFGDVENIMDLASIGQNTAIFKGIFQAKVDGKTAQEIVEMANKIS
                     FSRENTKPIAERAGVPITADDFLNAQRDGQKRAGNPILADDFLNTKRDE"
     gene            1941..2729
                     /locus_tag="PK513_08885"
     CDS             1941..2729
                     /locus_tag="PK513_08885"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOO82605.1"
                     /translation="MNKKKLVINETILKSRLFLGTAGYPSPQALGESIEAAQPGLITV
                     SLRRESKAGKGQDFWNILKNFDVPILPNTAGCHSPEEAITTAEMAREVFETSRIKLEI
                     IGDQYTLQPDPFGLLEATEALLKKGFEVYPYMTDDLVLAQRLADLGCKILMPWGAPIG
                     SGRGLNNPYALKQLRERFPNITLVVDAGIGAPSHAAQVMEMGYDAVLLNTAVAKAGHP
                     AEMARAFAQSIEAGRAAYLAGIIAPQENATPSTPLIDKPFWHQQ"
     gene            complement(3146..3484)
                     /locus_tag="PK513_08890"
     CDS             complement(3146..3484)
                     /locus_tag="PK513_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOO82606.1"
                     /translation="MAITRDFKETIMERAKRDAGFRRGMLMRGIAMLIAGDAEDVQVG
                     KSYIRDYINATIGFPKLSKRTGIPKESLMRMLSPSGNPSLSNLNQVTHTLLETEGVKE
                     ADSLRVSVQG"
     gene            complement(3559..3861)
                     /locus_tag="PK513_08895"
     CDS             complement(3559..3861)
                     /locus_tag="PK513_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO82607.1"
                     /translation="MAKIVEYKDHFSKWLSRVPAKHALRVTEALYRMELGNFGDHKSV
                     GEGVMERRIFGSPAIRIYYAMDGKELIVLLVGGTKSKQDKDIAKAKKLWGAYKSEK"
     gene            complement(3865..4209)
                     /locus_tag="PK513_08900"
     CDS             complement(3865..4209)
                     /locus_tag="PK513_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HOO82608.1"
                     /translation="MKLTNTACKNAKPTNKAVKMFDGQGLYLEVTTAGNKLWRLKYRY
                     NGKEKRIAFGAYPEVSLAEAREKRDKARKLLAEGHDPSLERKKRKAEILENAENTFEK
                     VAREWHEFNING"
     gene            complement(4502..>5365)
                     /locus_tag="PK513_08905"
     CDS             complement(4502..>5365)
                     /locus_tag="PK513_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82609.1"
                     /translation="KSYAAIDPKDLPDLLRAIAIPAKLALYFPYREYKKSLKTEDFKQ
                     ARSKCWRMSNAALSFFGQITQVAMHLSEEKIKEIVRNHFEKRLLDASEEYYFHTSLVR
                     NDPVEGFSPGVIKHMKGESEKLLSHYDSLKASHDYDDEQESIAQEILKFENIDSEANK
                     TEFFQLCLELVRAHYEAERINHAYLHAQEHEGKITDPYFEGCSNFFEQRAPEIFFEYR
                     GKPYYRATASSTLEDAYILFSKSGTISSSLYNPFHSTYFLSWTLLWLLKTCMLKNATP
                     VRIPFLPNCLN"
BASE COUNT         1435 a         1358 c         1190 g         1382 t
ORIGIN      
        1 aggttatgag cctgacgagc taccgggctg ctccatcccg cgatacaaaa aaacaaaaga
       61 ccgcaaggcc cttcaaagaa tggatggtga tatatagcgc cccgcccggt taaaagcaag
      121 tcacatcaat tgacttttta caaaataagc ataaattggc gaaaacactc accggcggtg
      181 cccataagga gctgagatta tacgcccggg acctgatcca gattgtactg gcgtaggaag
      241 cgtgagacga aagatgagcc aacaaccaca aaacatagcc attatcggcg ccggcattac
      301 tggactctgc tccgcctata ccatgcttga ggatggccat ggcgtaacac tctatgaccc
      361 cgcaggcttc ccggccgata acgccagctt catggctggc ggcatgctcg cgcctttggc
      421 cgaaatcgaa catatgcccc atgaatggat cgaagcgggc ttaggcagca tcgccctgtg
      481 ggccaaattt gcagaaaagc atggcgatgg cctaggtttt gactttcgcc aaaacggcag
      541 cctcctgctc gcccatgagg aagatcgcta tatcttacag cgctttgccg cccacctgcc
      601 cgccggtaag ggtgaatata ccgctactag tgagctcgaa ccagcactgg ctgaaaaatt
      661 cccgcaaggg ctatatctgg ctgaagaagc ccacattaat cctgccccgg ccatgcacgc
      721 cttgtgtgat tatctcaaag ccgcaggcgt aaccttcatt caggaaaaag ccgaccccac
      781 ggccataagc gcacaatacg accatgtcct tgactgccgc ggcatgggcg cggcatggga
      841 agacaaaaac ctgcgcggcg ttaaaggcga aatcgtcatc gtccacaata ccgaactggc
      901 cttaaaccgc ccggtccgcc tgatgcatcc gtgctatccg ctctatatcg tcccgcgccc
      961 tgaccacatc ttcatgatcg gcgccaccat catcgaaagt tctgacaata cacaggtcag
     1021 catccgctcc ggcctagagc tcctcagcgc cctgtacagt ctgcacccgt ctttcagcga
     1081 agccgaaatc attgaagtta aggccggcat tcgcccatcc taccccgaca atttgccgcg
     1141 catagaaata tcaaaaaatg acaacatcat ccgctgtaac ggcctgtttc gccatggctt
     1201 tatgctttct ccaataatag gacggtgcgt agcggatcat attgcgggcg gacaaaataa
     1261 tttcctcaac ctgctaaaga aagaccgcga tgaacatcat catcaacggg cggcctaaaa
     1321 acttcaccgc cccgctcaac ctctatgacg tgctcgcaca agaagaagtc atcggcatga
     1381 tgatcgccgt ggcgcgtaac ggcaccttcg tgcccaaagg cgattatgcg cacactatcc
     1441 tcaaagatgg cgacgacatc gaaatcgtcg ccccgatgca gggagggtag aaatgaagaa
     1501 aatatttttg ttactagcta ttttactttc tgtttcagca tgtctcaaag aaacaagaat
     1561 tgacgcttca agtgaagaaa agctacaaaa aagcattgca gaaattatga aagaactcga
     1621 accagaccaa cagaagaatt tctcacaatc tttgaaaatt attatgtttg gtgatgttga
     1681 aaatattatg gacttggcct ctataggaca aaacaccgca attttcaaag gcattttcca
     1741 agcaaaagtt gatggaaaaa ctgctcaaga aattgtagaa atggcaaaca aaataagttt
     1801 ttcccgtgaa aacacaaaac caatcgctga aagagctggc gtacccatta cggcggatga
     1861 ttttttaaac gcgcaaagag atggacaaaa aagagctggt aaccctattt tagcagatga
     1921 ttttttaaat actaaaagag atgaataaaa aaaaactcgt cattaacgaa accatcctca
     1981 aatcccgcct gtttttgggc acggctggtt acccgtcacc acaggcctta ggcgaaagca
     2041 ttgaggcggc acagcccggc ctgatcaccg tgtctttgcg ccgcgaaagc aaagccggta
     2101 aaggtcagga tttctggaac atccttaaaa atttcgacgt accgatcctg cccaataccg
     2161 ccggatgcca cagccccgaa gaagccatca ccacagccga aatggcccgc gaggtttttg
     2221 aaaccagccg tatcaaactt gaaatcattg gcgaccaata caccctgcaa cccgacccct
     2281 tcggcctgtt agaagccacc gaagccctcc tgaaaaaagg cttcgaagtc tacccctata
     2341 tgaccgacga tctggttttg gcccagcgcc ttgccgatct gggctgtaaa atcctcatgc
     2401 cctggggcgc gccgatcggc tctggccgcg gcctcaacaa cccttatgcg ctgaaacaac
     2461 tgcgcgagcg ttttcccaat atcaccctcg ttgtcgatgc cggcatcggc gcgccatcac
     2521 atgcggctca ggtcatggaa atgggctatg acgccgtttt actcaacacc gctgtcgcca
     2581 aagccggaca ccccgccgaa atggcccgcg ccttcgccca aagtatcgag gctggccgcg
     2641 ccgcctatct ggcggggatc atcgccccgc aggaaaacgc caccccttca accccgctga
     2701 tcgataaacc cttctggcac caacaataga tacccacggg taaacccgtg gttctatggg
     2761 gctgcgccgc gctttgcgca ttacaattcc tcgcggaatt gacctgaatc ccggtgtcct
     2821 tgacttcctg cgcaacggca agaacgcgga cggtagcccg gtctatgggg tgcctaacac
     2881 tgggtgccag tgttcctaat tccctcctgt aagtgcctga taaaaaagat ggatatttcg
     2941 cgcggattct ttatgcttgt ccgtgatctg ttgtacgggc aggtctgggc ttaagaaccc
     3001 aaatccaaag tggcaggtac atttcgcgtg tcgaaagttg gaaagagggt tatatagcgg
     3061 gctatttaga aggtgcaaaa ttgcttattg gtacatggcc tgaaaacaag gaaacacttt
     3121 gtcaaagcag cttaagtgcg ctcaattaac cctgcacaga aacccgtaag ctatcagcct
     3181 ctttcacacc ttctgtttcc agtaaagtat gggttacttg atttagattg ctgaggcttg
     3241 gattgccaga agggctcagc atgcgcataa ggctttcttt tggtatgccc gtgcgttttg
     3301 acagtttagg aaagccaatc gtcgcgttga tataatctct gatataggat ttacccactt
     3361 gaacatcttc cgcatcgccc gcaataagca tagcgatgcc gcgcataagc atgccacgac
     3421 gaaaaccagc gtcgcgtttt gccctctcca taattgtttc tttaaaatcg cgtgtaatag
     3481 ccatttttca ccttacccat ttctattttt atgctaacat atatgttatt atttggcaag
     3541 tcaaatgtgt taaaacgctt atttttcaga cttataagca ccccaaagct ttttagcttt
     3601 agcaatatcc ttgtcttgct tactttttgt gccgccgacc aaaagcacaa taagctcttt
     3661 accatccata gcgtaataaa tgcgaatagc gggactacca aaaatccgcc gctccatcac
     3721 gccttcaccg actgatttat gatcgccaaa gttccctaat tccattcggt aaagggcttc
     3781 cgtaacacgg agcgcgtgtt ttgccggaac tcttgaaagc cattttgaga aatgatcttt
     3841 gtactcgact attttagcca ttggtcaacc gtttatgttg aactcgtgcc attcgcgcgc
     3901 gactttctcg aaggtattct ctgcattttc aaggatttca gctttccgct tcttgcgttc
     3961 taaggaagga tcatggcctt cagcaaggag ttttctggcc ttgtcccgtt tctcccgcgc
     4021 ctcagctaga gatacctcag gataagcgcc aaatgcgatt ctcttttctt tgccattgta
     4081 acggtatttc aagcgccaga gcttgtttcc tgccgttgtg acctcaaggt aaagcccttg
     4141 gccgtcgaac atcttaacgg ctttgttggt cggtttggca tttttgcatg ctgtattggt
     4201 cagtttcatg tgggggcaag ctcatttctg ctttgggggc atgcacccta gcttgccccc
     4261 attttgtccg attgcacgca atctcttgcg aacagaaatg attatacatg aaagctgaaa
     4321 ccgttgcaaa cgctagcgtt ttcggtcatc agtgaacatc tatgaatgtc ggtgaagagt
     4381 aaaattggcg tcccgtacgg gagggggtgc caattttttg aaccccttgt aaaccgcagt
     4441 tttccaagtt tcgctattgt taaaattaac caaaatgtac aaatctctgt acaaaaatca
     4501 actaatttaa gcaatttggt aaaaatggaa ttcgaactgg tgttgcattt tttagcatgc
     4561 aagttttcag aagccataat agtgtccaac ttagaaaata ggtgctatga aatggattgt
     4621 agagagaaga tgaaatggtt ccagattttg aaaatagaat ataggcatct tctaaagtac
     4681 ttgatgctgt tgcgcgataa taaggtttcc cccgatattc gaaaaaaatc tccggagcac
     4741 gttgttcaaa aaagtttgag cacccttcaa aataaggatc ggttattttt ccttcatgtt
     4801 cttgagcatg taagtaggca tgattaatac gctcagcctc ataatgcgcc cttactaact
     4861 caaggcaaag ttgaaagaac tcggttttat ttgcctcact atctatattt tcaaatttta
     4921 gaatttcttg ggctatactt tcctgctcat catcgtaatc atgagaggct ttgaggctat
     4981 cataatggct taatagcttt tcgctttcgc ctttcatgtg tttgataaca ccggggctaa
     5041 agccttcaac ggggtcattg cgaaccaatg atgtatgaaa atagtactct tctgatgcat
     5101 ctaaaagccg cttttcgaag tgattgcgga cgatttcttt gattttttct tctgataaat
     5161 gcattgccac ctgcgtaatt tgcccaaaaa aggatagcgc ggcattgctc attctccaac
     5221 actttgaacg agcttgtttg aagtcctcgg tttttagaga ctttttgtat tctctgtagg
     5281 gaaaatacag tgccagtttt gcaggaatag ctattgctct taaaagatcg ggtaagtctt
     5341 tcgggtctat ggcggcatat gattt
//
LOCUS       DAOSWR010000132         5363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10225_length_5363_cov_4.907121, whole genome shotgun sequence.
ACCESSION   DAOSWR010000132 DAOSWR010000000
VERSION     DAOSWR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10225_length_5363_cov_4.907121"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="tolB"
                     /locus_tag="PK513_08910"
     CDS             <1..348
                     /gene="tolB"
                     /locus_tag="PK513_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566982.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HOO82610.1"
                     /translation="NVQRITFGQGRYANPVWSPRGDLIAFTRMYKGKFYIGVIRPDGK
                     GERLITNAYHVEGPSWSPNGRVLTYFKERPVGPGGTQRQAKIYTVDITGYNERWLRTP
                     QDGSDPAWSPLNP"
     gene            complement(416..892)
                     /locus_tag="PK513_08915"
     CDS             complement(416..892)
                     /locus_tag="PK513_08915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HOO82611.1"
                     /translation="MDKSALPYRPCVGIALFNAQGHVFVGERIDTPGAWQMPQGGIDE
                     GEDLEVAARRELCEEIGTDSAELLKISEQTTRYDLPAHLLGRLWDGRYRGQEQHWVAM
                     RFTGEDSDIRLDADQRPEFKAWQWVALSDTLKLIVPFKQDTYQRVIAMFSELSLRA"
     gene            complement(903..2030)
                     /locus_tag="PK513_08920"
     CDS             complement(903..2030)
                     /locus_tag="PK513_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOO82612.1"
                     /translation="MELMHSIQKRFSFKSFARGVFVLLAVYGALFGYAFLQADVTREY
                     LQSKLASQTVLIDGLSETGFIDAQDHLGAEDLVEAPSSAHGSEHSAIENISHEDNSSL
                     KDVVIQDAKSLGKAPVEGLFEDSAFGRLPIAKNAVETPFAAYRKPFVLNQERPFIAVV
                     VEQFGLSNALSEEMIAALPASVSFVLTPYSQNPERWVSEARADGHEVWLHLPMENKNF
                     PMEDPGAKGLLTRVSLQYNQDRIQWLMGRITGYVGVAAYTDDTLDNAGQMFKNMANDL
                     FRRGLGFLELNSEEKSFFEPIAEEMHTPHTAVQSYIDVVDPQRPAIKKALKQIDAQGG
                     TLVVVRPSPRNIPALKRWLESLQKRGIQIVPVSAVAAANNY"
     gene            complement(2040..3380)
                     /locus_tag="PK513_08925"
     CDS             complement(2040..3380)
                     /locus_tag="PK513_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO82613.1"
                     /translation="MVFTFHGVFAQNVDEPRTAGTQPAVEDHSDTYRQLDLFGDVFER
                     VRAQYVEETADHELIESAINGMLTALDPHSSYLNNEKFEDMQISTRGEFGGLGIEVTM
                     ENGVIKVVSPIDDTPAFRAGVLAGDYIVQIDDEPVMGLTLSEAVDKMRGKVGTEIDLL
                     ITRESADAPIEMTLVRDIIKIRSVRHRVEGNSGYVRITTFNQNADSGVKKAIQEIKAE
                     LGNKLNGYVLDLRNNPGGLLDQAIAVSDIFLDKGEIVSTRGRHEDDTKRDNATPGDLA
                     EGLPIIVLINGGSASASEIVAGALQDHRRAILLGTQSFGKGSVQTVIPLPGHGAMRLT
                     TARYYTPSGRSIQAKGIEPDILVEPAKIESYNIKRLREENLKGALDRKAVKDDSESTE
                     ESEGEDEEVQDYQLMRALDLLNGLMLYKGEPRAVNDNAPAPEAEPSAAETQSQP"
     gene            complement(3419..4537)
                     /locus_tag="PK513_08930"
     CDS             complement(3419..4537)
                     /locus_tag="PK513_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO82614.1"
                     /translation="MPAAMVQAQDAPVPPKKENLDTKLKNTKANKKALEADLKTIEKD
                     LNATKNSLVKTAKAIQKNEKELQNLDLKIADLEKEEKSLSEKLQNDRGSISRLILALE
                     RIRRLPPEALIVRPEAPLKTAQSAMLLEEILPALNKQATDLKVTLENLQSVSEDLQAK
                     RAAMVESSKKLEAQQRELNAMVKKRTRLYASTRNDIAAQQKKVAKISAQAKNLADLVS
                     RLDADRARGQEVAQANKKRIVRQEPPKSGQPRLPLPGIIKTGYNEPDNFGAPSKGLDI
                     EGRAGALVVAPMGGIIRFAGYFKNYGNMVILEHQKGWHSLIAGLENIDTVVGQSVGAG
                     EPLGVLHKSSTSQRPVLYYELRHKGKAVDPAKKFGDLS"
     gene            complement(4590..5045)
                     /locus_tag="PK513_08935"
     CDS             complement(4590..5045)
                     /locus_tag="PK513_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOO82615.1"
                     /translation="MNIEILACGRMKKGAHADLFQDYLKRMDWNVHVHEIESRYKEQK
                     KIHSDECVQILARIKPDCFVIALDERGKTMPSREIAAKFEMLQNESHAQVQCILGGAD
                     GLNDTIRARADFLLSFGRQTWPHMLARVMLLEQIYRSQQILKKHPYHRD"
     gene            complement(5063..>5363)
                     /gene="rsfS"
                     /locus_tag="PK513_08940"
     CDS             complement(5063..>5363)
                     /gene="rsfS"
                     /locus_tag="PK513_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOO82616.1"
                     /translation="DIVSIKLDEQTGLADYMIIASGTSSRHVSALTDKILKRLALNGI
                     KSVGVEGKDRSDWVAIDAGDVIIHLFRPEVRNFYNIEKMWGNFQPFEVVRDPLHA"
BASE COUNT         1380 a         1474 c         1131 g         1378 t
ORIGIN      
        1 aatgtgcagc gcatcacctt cggacagggg cgctatgcca acccggtctg gtcgccgcgt
       61 ggcgatctca ttgccttcac gcgcatgtat aagggcaaat tctatatagg cgtcatccgc
      121 ccagatggca aaggtgaacg tctgatcacc aatgcctatc atgtcgaagg tccaagctgg
      181 tcgcccaatg gccgtgtcct gacatatttt aaagaacgcc ccgttggccc aggcggaacg
      241 cagcgccagg ccaaaatcta tacggtggac ataacaggat ataacgagcg ttggctgcgc
      301 accccgcaag acggctccga cccggcctgg agtccgttaa acccataaag gcgagagcag
      361 aaaagtcatt gcgagaaggc cgtaaggccg acgctacaat ccatagattg cttcgctacg
      421 ctcgcaatga cagctcagaa aacatcgcaa taactctctg ataagtatct tgcttaaacg
      481 gcacgatgag tttgagcgta tcgcttagag ccacccactg ccaggcttta aattccgggc
      541 gctgatcagc atccagccga atgtcgctgt cctcgccggt aaaccgcatc gccacccagt
      601 gctgttcctg ccctcgatag cgaccgtccc agagcctgcc cagcaaatga gcaggtaaat
      661 catagcgcgt agtttgctcg ctgattttca acagctccgc gctatccgta ccaatttctt
      721 cgcacagctc tcgccgcgcc gccacttcca aatcctcacc ctcgtcaatg ccaccctgcg
      781 gcatctgcca ggcaccgggc gtatcaattc gttcaccgac gaaaacatgc ccctgagcat
      841 taaataaagc gatcccgaca cagggtctat agggcagggc gcttttatcc attttaaaat
      901 ccctaataat tatttgccgc cgccacagcg gaaaccggta cgatctggat accacgtttt
      961 tgcaaagact ccagccaacg tttgagcgcc ggaatattac gcggagacgg gcgcacaacc
     1021 accagcgtcc cgccctgcgc atcaatctgc ttcaacgcct ttttaatcgc cgggcgttgc
     1081 ggatcgacaa catcaatata gctttgtacg gccgtatgtg gagtgtgcat ttcctcggct
     1141 atgggctcaa aaaaactttt ttcttcgctg ttcaactcaa ggaagccaag cccgcgccta
     1201 aaaagatcat tcgccatatt tttaaacatt tgtccggcgt tatccaaagt atcgtcagta
     1261 tacgctgcca cgccgacata gccggttatc cgccccatta accattgaat acgatcctga
     1321 ttatattgaa ggcttacccg cgtcagcagg cctttcgcac caggatcttc cattggaaaa
     1381 ttcttgtttt ccatcggtaa atgcagccaa acctcatgcc cgtcggcacg cgcttcacta
     1441 acccatctct caggattctg gctataaggt gtaagaacaa aagaaaccga agcaggcaac
     1501 gccgcaatca tttcttccga tagagcattc gaaagaccaa actgttctac gactacggca
     1561 ataaagggcc gctcctgatt aagcacaaac ggtttacggt aagccgcaaa aggcgtctca
     1621 accgcattct ttgcaatggg gagacgacca aacgcgctgt cttcaaacaa gccttcaacg
     1681 ggtgccttac caagcgactt agcatcttgg atgacgacat ccttcaagct gctgttgtct
     1741 tcatgagaga tgttctctat agcgctatgc tctgaaccat gtgcagaaga aggtgcttca
     1801 acgagatcct ctgcgcccag atgatcttgc gcgtcaataa aacctgtctc gctcagacca
     1861 tcaatcaaaa cagtctgact tgccagcttg gattgcagat attcccgcgt aacatcagcc
     1921 tgcaaaaagg cataaccaaa caaggcgcca tagacagcca gaaggacaaa cacgccacgc
     1981 gcaaagcttt taaaagaaaa acgcttctga atgctgtgca tcagttccat aaaaatcctt
     2041 tacggttgac tttgcgtctc tgctgctgac ggttcagcct caggggcagg agcgttgtca
     2101 ttcaccgcgc gcggctcacc tttatacagc atcaggccat taagaagatc aagagcgcgc
     2161 atcagttgat aatcctggac ttcttcatcc tcaccttcgc tctcttccgt actctcacta
     2221 tcatccttca cggcttttct atcaagtgca cctttcagat tttcttctcg caaacgttta
     2281 atattatagg attcgatctt agcaggctca acaaggatat ccggctcaat gcctttagcc
     2341 tgaatagagc gccccgatgg cgtatagtaa cgagctgttg tcaaacgcat agcgccatga
     2401 ccgggcaacg ggattactgt ttgtacagag cctttaccaa aagattgtgt tccaagcaaa
     2461 atcgcacggc gatggtcttg caatgcgccg gcaacaatct ccgatgctga agccgatcca
     2521 ccattgataa gaacgataat tggcaaacct tcggccagat cgcccggtgt agcattgtca
     2581 cgcttggtat catcttcatg tcgcccgcgc gtcgagacaa tctcgccctt atctaaaaat
     2641 atatcagaaa ccgcaatcgc ctgatccagc aagccaccgg gattattacg caaatccagc
     2701 acataaccat tcagcttgtt accaagctca gccttaattt cctgaatagc ctttttaaca
     2761 ccgctatctg cattttggtt gaaagtggta atgcgtacat agccggagtt gccttcaact
     2821 cggtgacgta cagagcgaat cttgatgata tcgcgaacca acgtcatctc aattggcgca
     2881 tctgcactct cacgagttat gagaagatca atctccgtgc caaccttgcc gcgcatttta
     2941 tcaacagcct ctgagagtgt taaacccatc acaggctcat catcaatttg cactatgtaa
     3001 tctccggcca gaactccggc acgaaacgca ggagtatcat caattggtga aaccactttg
     3061 ataacgccgt tttccatcgt aacttcaatt cccaagccgc caaactcgcc gcgtgtgcta
     3121 atctgcatat cttcaaattt ttcattatta agataggatg aatgcgggtc cagcgccgta
     3181 agcatgccat taatagcact ttcgatcagt tcatggtctg ctgtttcctc aacatattgc
     3241 gcgcgcacgc gctcaaacac gtcgccaaac aaatcaagct gacggtaagt atcgctgtga
     3301 tcttctacag caggctgtgt gcctgctgtg cgcggctcgt ccacgttctg tgcaaaaaca
     3361 ccgtgaaatg taaacaccat aagggtaaga gcaacaagaa acagtgtcag tatgcgcatc
     3421 agcttaaatc tccaaatttt ttggccgggt ccacggcttt gcctttatgg cgcagctcat
     3481 aatacaaaac aggtctttga ctggtagaac ttttgtgaag cacacccaaa ggctcgccag
     3541 ctccaacaga ttgtccgacc actgtatcaa tattttccaa acctgcaata agactatgcc
     3601 atcctttttg atgttcaagg atcaccatat tgccataatt tttaaaatac ccggcaaaac
     3661 ggataattcc gcccattggc gcaacaacca aagcccccgc gcgtccttca atatcaagcc
     3721 ctttgctcgg tgcaccaaaa ttatctggct cattatagcc ggtcttgatt ataccgggca
     3781 ggggcaggcg tggctgccca gactttggcg gctcttgtct gacaatacgt tttttattgg
     3841 cttgcgctac ttcctgtcct cgtgcgcggt cagcatccaa acggctaaca agatcggcaa
     3901 gatttttcgc ttgtgcagaa atctttgcaa cctttttctg ctgcgcagca atatcattgc
     3961 gcgtactggc ataaagccgg gtacgctttt taaccattgc attcagttcg cgctgctgcg
     4021 cctccagctt ttttgaagac tcaaccatag cggcgcgttt agcctgcaaa tcctctgata
     4081 cactttgcag attctcaagc gttactttca gatctgttgc ctgcttgttc agggccggta
     4141 aaatctcttc cagcaacatc gcgctttgcg cagttttgag tggcgcttcg ggacgcacaa
     4201 taagcgcttc tggcggcaag cggcgaattc gctccagcgc caaaataagc cgtgaaatcg
     4261 acccgcgatc attttgtaat ttttctgaaa gagatttttc ttccttttcc agatccgcaa
     4321 ttttcaaatc cagattttgc agttctttct catttttctg aatcgccttg gccgttttga
     4381 ccagagaatt tttagtcgcg ttcaaatctt tttctatcgt cttaagatca gcttccagcg
     4441 cttttttgtt ggcctttgta tttttcagct tggtgtctag gttttctttc ttcggcggaa
     4501 ctggggcatc ttgagcctgc accatcgcag caggcataaa cagaaacaat aaaactagaa
     4561 acaggcgcat agaacttagg tctcaaaatt taatcccgat gatagggatg ttttttcaaa
     4621 atttgttggc tcctgtaaat ctgctctaaa agcatcacgc gtgcaagcat atgcggccat
     4681 gtttggcggc caaagctgag gagaaaatcg gcacgcgcgc gaatcgtatc attcaaacca
     4741 tccgcaccgc ccaaaataca ctgcacttgc gcgtggcttt cattttgcaa catctcaaat
     4801 tttgccgcaa tctcacgcga tggcatggtc ttgccgcgtt catccaatgc aatgacaaaa
     4861 caatccggct taatacgcgc caaaatctga acgcattcat cgctgtgaat ttttttctgc
     4921 tctttataac gggattcaat ctcgtgaacg tgtacattcc aatccatccg cttcagataa
     4981 tcctgaaaca gatccgcatg cgcacctttt ttcatacgcc cacaggcgag aatttcaata
     5041 ttcatgaaat atatactaag cgttatgcgt gtaacggatc tctgacaact tcgaagggct
     5101 ggaaattccc ccacattttt tcgatattgt aaaaattgcg gacctcaggc ctgaacagat
     5161 gaatgatcac atcccctgca tcaatagcaa cccagtccga tcgatccttg ccttccacac
     5221 caacgctctt aataccgttg agcgccagtc gcttaagaat tttatctgtc agcgcactca
     5281 catgacgtga cgacgttcct gatgcaatga tcatataatc agccaatccg gtttgctcat
     5341 ccagtttaat gctgacaata tct
//
LOCUS       DAOSWR010000133         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10462_length_5270_cov_4.439693, whole genome shotgun sequence.
ACCESSION   DAOSWR010000133 DAOSWR010000000
VERSION     DAOSWR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_5270_cov_4.439693"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..843
                     /locus_tag="PK513_08945"
     CDS             172..843
                     /locus_tag="PK513_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82617.1"
                     /translation="MKKIFRLSSVCALALCAGVSSAAADVAMWRDVGSKMSLTYDDRW
                     DAVSNQNPGDVLTIRAPNTAAHGLYNFAGCKVNIENDGRFKIYPRRNAGTIQRTAFSR
                     DYWERYFGLYNDVVVHEGTDNNGLGEGFASMASFSFEDYKGAKVKKRGIAFASHYRNR
                     VYLVECSAEESVYHEWHNAFLNVIKSVDFHDGTNFAITGYYRDFLQDKTLKVRGPSVF
                     EDGYY"
     gene            943..1860
                     /locus_tag="PK513_08950"
     CDS             943..1860
                     /locus_tag="PK513_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO82618.1"
                     /translation="MQIYLPIAEMAVSAETVFMLSAFVGLLSGIFGVGGGFLTTPFLI
                     FFGIPPAVSVATQASQLVASSMAGAMGHFRRGNVDIKMGLVMVSGGAVGSVVGIFIFK
                     LLRYLGQIDFAISFLYIILLGGIGILMIMESFVSVLKKKEGVRKAFNDSKVSPWVLSL
                     PYKMRFPRSKLFVSALVPAGIGFVGGVLASVLGIGGGFLIVPAMIYILGMPPLLVAGT
                     SLFQVIFTTAAATIMHALMNHTVDIVLGLILILGGVVGAQFGVVLARFFKGQTARVTL
                     AIIILSVCLKLVVDLFVMPVDLFSAEYVR"
     gene            1878..2639
                     /locus_tag="PK513_08955"
     CDS             1878..2639
                     /locus_tag="PK513_08955"
                     /inference="COORDINATES: protein motif:HMM:NF021148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HOO82619.1"
                     /translation="MNSFSAIAFFFVLGVYVVSAFSEIAAAEPLQFDIADDYVEITTG
                     FTGTTLSMIGYRKGRGDIVLTLEGPPRSTVVRQKSQVFGAWVNRETLQFDNIPSYYDF
                     ALSVDDEKKLMSDELLKEYHVGLNALRVVPKEKRIDEDKIKLFQSALLRYKQQHQLYS
                     QKAGAVEFLGDDFFRVDFHLPAHVPAGNYTVRALLVGKGKVVYEDAQSLRVGLTGFSS
                     DIYEFSRNHAFLYGALCVLIACFAGWLSNFIVRRS"
     gene            2673..3161
                     /locus_tag="PK513_08960"
     CDS             2673..3161
                     /locus_tag="PK513_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO82620.1"
                     /translation="MTKAETKKDDVRSLLPDTDGGVFLVVVDDSSEFDAALRRVATLV
                     KQKRGHAAVLYVIEDEGFLHWNFIEKQIQRDKRAEAEEALWKAAQRFYELSGCIPGIY
                     IKEGKTYREIAQILNTDPTIRALVLGASSSSSNPLISYFTGKGLSELRVPLMIVPETL
                     NS"
     gene            3218..3784
                     /locus_tag="PK513_08965"
     CDS             3218..3784
                     /locus_tag="PK513_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOO82621.1"
                     /translation="MFIQTEATPNPATVKFLPGQSVLDRGTAEFKTTGEASEAPLVQR
                     LFGLQGVEGVFLGADFISVSKDEDTDWSVLKPMVLGALMEHFSTGQPVVLEAYYQGKG
                     LSEDKGKDDEISAQIRELIETRVRPMVAMDGGDIVFDRFEDGVVFLHMRGACSGCPSS
                     TATLKMGIENMLKHFVPEVVEVRPAENF"
     gene            complement(3696..4826)
                     /locus_tag="PK513_08970"
     CDS             complement(3696..4826)
                     /locus_tag="PK513_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO82622.1"
                     /translation="MNRLLLQCALTLLLIAVLLSPVYSAASESTQNHAVILTYHRIGE
                     DNLPDENLTIEQFTTHIHEIKTGGYNVLPLTTILEDFETGTLLPPHTIAITFDGAHKS
                     ALENAVPLLLKAKLPFTIFYAALNLDQEDPASANWNDLKKLAKNKNITLASLPTSYNH
                     IAYKTRTEMLTALNEARRRHREEFGHETPYLAYPFGEYSLALKDLAKTQGFKAAFGLH
                     SGAIDATSDRFALPRFSMTEHYGDLERFRLVTRAIPLTVTDIEPPDPDLKTAPFFTGF
                     TLPDALASKSKNLSCFISGEGRAKTEILGNRIEIREDTGLLDNDRIRLNCTLPGPLSE
                     NDEPQWYWLGLLYFRSSQPDELQRPQEQNASTYSQYPSSKSP"
     gene            4882..>5270
                     /locus_tag="PK513_08975"
     CDS             4882..>5270
                     /locus_tag="PK513_08975"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HOO82623.1"
                     /translation="MDEKMKDLLFREARTHNVWLEKDVPDALLREIYDLMKFGATAAN
                     SCPARIVFVKTPEAKARLKPHLDEGNVEKTMAAPVTAIIAHHLEFYEKLSQLFPHADA
                     RSWFAGNEALIAESAFRNGSLQGAYFM"
BASE COUNT         1106 a          913 c         1603 g         1648 t
ORIGIN      
        1 aaacccataa tatcccgaac ttcagctacg gttctgttcg cgatattatg ggtttctggc
       61 acgtcggtta accggttatt catattccct ctggcaaaat ggttgtgttt gaggcataat
      121 catgggagtt cacggcgttt cagccaccca tattttcttt ggggcaatca gatgaaaaaa
      181 atatttagac tatcaagtgt ttgcgcgttg gctctttgcg ccggtgtatc ctctgccgcc
      241 gcggatgttg ctatgtggcg ggatgtgggc tctaagatga gcctgaccta tgatgatcga
      301 tgggacgctg tttccaatca aaatccgggg gatgtcttga ctattcgcgc tccgaatacg
      361 gcggcgcacg gactgtataa ttttgccggg tgcaaggtga atattgagaa tgatggacgg
      421 tttaagattt atccgcgccg gaatgccggg accattcagc gtacagcctt tagcagggat
      481 tactgggagc ggtattttgg gctgtataat gatgtcgttg tgcatgaggg gacggacaat
      541 aacggtttag gcgaggggtt tgcctcgatg gcgtcatttt cgtttgaaga ttataaaggt
      601 gcgaaggtga aaaaacgcgg gattgccttt gcttcgcatt atcggaacag ggtttatctg
      661 gttgaatgtt cggcggaaga aagcgtgtat cacgaatggc ataacgcgtt tttgaatgtg
      721 attaagtcgg ttgattttca tgatggaaca aattttgcga ttaccggata ttatcgtgat
      781 ttcttgcagg ataagacgct gaaagtgcgc gggccgagtg tttttgagga tggctattat
      841 tagttatcct gtttttttca gttttatgtc ataaaatctt gaaaatttga cagtttttcg
      901 gcactgtggg ggcacagtaa ggaaacacga tacttctagt ccatgcagat ttatttgccg
      961 attgctgaaa tggcggtttc tgcggaaacc gtttttatgt tgagcgcgtt tgtcgggctc
     1021 ttgtctggta tttttggcgt tggcggcggt tttttaacaa cgcccttctt aatatttttt
     1081 ggtattcctc cggcggtgtc ggtggcaacg caggccagcc agcttgtggc ttcgagtatg
     1141 gccggagcga tggggcattt tcggcggggt aatgttgaca tcaagatggg gttggtgatg
     1201 gtttccggcg gggctgtggg gtcggttgtg gggattttta tctttaagtt gttgcgttat
     1261 ctggggcaga tcgattttgc aatttcattt ttatatatca ttttgcttgg cggtatcggg
     1321 atacttatga ttatggaaag ttttgtgtcc gtgcttaaga aaaaggaggg cgtgcgcaag
     1381 gcttttaatg atagcaaggt ttctccctgg gttttgtcgt tgccttataa gatgcgtttc
     1441 ccccggtcaa agctgtttgt gagtgcgttg gtgcccgccg ggattggttt tgttggcggg
     1501 gttctggctt ctgttttggg gattggcggc ggttttttaa tcgttccggc tatgatttat
     1561 attttgggta tgccgccttt gctggtggct ggcacgtctt tgtttcaggt gattttcaca
     1621 acggctgcgg caacgattat gcatgcattg atgaaccata ctgttgatat tgttttaggg
     1681 cttattctta ttttaggcgg cgttgttggt gcgcagttcg gggttgttct tgcccggttc
     1741 tttaaggggc aaacagcgcg tgttactttg gcgatcatta ttttgtcggt gtgtttgaag
     1801 cttgttgtcg atttgttcgt tatgccggtt gatctgttct cagcggagta tgttcgatga
     1861 cctgctgtcg acgttttttg aattctttta gcgcgatagc gttctttttt gtacttgggg
     1921 tttatgttgt tagcgctttt tcggagattg cggcggcgga gcctctgcaa tttgatattg
     1981 ctgatgatta tgtcgaaatt acaacgggtt ttaccggtac aactttgtcg atgatcggat
     2041 atcgaaaagg gcgtggggat attgttttaa cgcttgaggg gccgccgcgt tcgactgtgg
     2101 tgcgtcagaa aagccaggtt ttcggggcct gggtgaatag ggaaactttg cagtttgata
     2161 atattccttc ttattatgat ttcgccttaa gcgttgatga tgaaaagaaa ttgatgagcg
     2221 atgagttgct taaggaatat catgttggtt taaatgcgtt acgggttgtg cctaaagaga
     2281 aacgtataga tgaggataaa attaagctct ttcagagcgc tttgttgcgt tataaacagc
     2341 agcatcagct ttattcgcag aaggccgggg cagtcgagtt tttgggggat gatttttttc
     2401 gggtggattt tcatttgcca gcgcatgtcc cggcgggaaa ctatacggtg cgggctttgc
     2461 ttgttggtaa gggcaaggtc gtgtatgagg atgcgcaaag tttacgggtg gggttgaccg
     2521 gttttagctc tgatatttat gagttttcac gaaatcatgc gtttttgtat ggggctctgt
     2581 gcgttttgat tgcatgtttt gccggatggt tgtcgaattt tattgtgcgg cgcagctagg
     2641 ataaaggctg tggatgcttt aaggtgtttt ttatgacgaa agctgagaca aagaaagacg
     2701 atgttcgctc attgctgccg gatacagatg gtggggtgtt tttggttgtc gttgatgata
     2761 gctcggagtt tgatgcggcg cttcggcgtg tggcgactct tgttaagcag aaacgcggtc
     2821 atgcggctgt tttgtatgtg atagaagatg aaggtttttt gcactggaac ttcattgaaa
     2881 agcaaattca gcgtgataag cgggcagagg ctgaggaagc cttatggaag gctgcgcagc
     2941 gtttttacga gctttcgggg tgtattcccg gaatttacat caaggaaggt aaaacatatc
     3001 gtgaaattgc gcagatcctt aatactgatc cgacgatccg cgcactggtt ttaggcgctt
     3061 cgtcttcgtc ttccaatcct ttgatttctt attttactgg taaggggtta agtgaattgc
     3121 gagtgccgct tatgattgtg cctgaaacgc ttaactcctg acttgatttt aatggtcgcc
     3181 tgtcttattt cagctatcgg aaagggactt tttactaatg tttattcaaa cggaagcaac
     3241 gccgaacccg gcgacagtta agtttttgcc tggacaaagt gttttggata ggggaactgc
     3301 ggagtttaag accacagggg aggcgtcaga ggcgccgttg gttcagcgct tatttggttt
     3361 acaaggcgtt gagggggtgt ttttaggggc agattttatt tcagtgtcca aggatgaaga
     3421 cacggattgg tcggtgctta aacccatggt tttgggagcc ttgatggagc atttttcaac
     3481 ggggcaaccg gtggtgctgg aggcgtatta tcaaggcaag gggctttccg aggataaggg
     3541 caaggatgat gaaatttcgg cgcagatccg cgaattgatt gaaactcggg tgcgtcctat
     3601 ggtggcgatg gatggcggtg acatcgtttt tgaccggttt gaagatggtg tggtgttttt
     3661 gcatatgcgt ggggcgtgtt cagggtgccc aagttctacg gcgactttga agatgggtat
     3721 tgagaatatg ttgaagcatt ttgttcctga ggtcgttgaa gttcgtccgg ctgagaactt
     3781 ctaaaataga gcagaccgag ccaataccat tgcggttcgt cattttcaga taggggaccg
     3841 ggaagtgtgc agtttaggcg gatgcggtcg ttgtctagca ggccggtatc ttcacgaatt
     3901 tcgatgcggt ttcctaatat ctcagttttg gcacggcctt cgcctgagat gaagcaggag
     3961 aggtttttgc tcttgctggc taatgcatcg ggaagggtga agcctgtgaa aaagggagcg
     4021 gtttttaagt ccgggtctgg tggctcgatg tcggtcacgg tcaggggtat ggcacgggtg
     4081 acgagacgga aacgctcgag gtctccgtaa tgttcggtca tactaaaacg cggcagggcg
     4141 aagcggtcac tggtggcgtc aattgcgcca gagtgcaggc cgaaggcggc tttgaaccct
     4201 tgcgtttttg cgaggtcctt aagcgccagg ctgtattctc cgaatgggta ggcgaggtat
     4261 ggcgtttcgt ggccgaattc ttcgcggtgg cgcctgcgtg cttcgttcag ggcggtcagc
     4321 atttctgtgc gggttttgta ggcaatatgg ttgtaggacg tggggagcga ggccagcgtg
     4381 atatttttgt ttttggcgag tttttttaaa tcgttccagt tggcgctggc ggggtcttct
     4441 tgatcaaggt ttagcgcggc ataaaaaatg gtgaatggta attttgcttt caaaagcaag
     4501 ggcacagcgt tttccagtgc ggatttgtgg gcgccgtcga aggtgatggc gatggtgtgc
     4561 ggtggcagta gcgtacctgt ttcaaagtcc tcaaggattg tcgtaagcgg caggacattg
     4621 tagccgccgg ttttaatttc atggatatgg gtagtgaatt gttcgattgt tagattttca
     4681 tcagggagat tatcttcgcc aatgcggtga taggtaagga ttacagcgtg gttttgcgtg
     4741 ctttcggatg cggcgctgta aaccgggctt aacagtacgg ctatgagcaa aagtgttaaa
     4801 gcgcattgaa gaagtaatct attcatgctt tttgtgtagt tgagttttgt cgtaaagaaa
     4861 aggatatatc gtatgagcgc tatggatgaa aaaatgaagg atttattgtt tcgagaggcg
     4921 cgcacacata atgtgtggct ggaaaaagac gtgcctgatg cgttattgcg cgagatttat
     4981 gatttgatga aatttggggc gacggcggcg aattcttgtc cggcgcggat tgtgtttgtg
     5041 aaaacaccgg aggccaaggc tcgtttaaag ccacatttgg atgagggtaa tgttgagaag
     5101 acgatggcgg cgccggtgac ggcgattata gctcatcatc tggaatttta tgagaagctg
     5161 tcgcagcttt tcccacatgc agatgcacgg agctggtttg ccggtaatga ggcgttgatt
     5221 gccgaaagtg cgtttcgcaa tggtagtttg cagggggcgt attttatgat
//
LOCUS       DAOSWR010000134         5264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10485_length_5264_cov_5.363985, whole genome shotgun sequence.
ACCESSION   DAOSWR010000134 DAOSWR010000000
VERSION     DAOSWR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10485_length_5264_cov_5.363985"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK513_08980"
     CDS             <1..662
                     /locus_tag="PK513_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOO82624.1"
                     /translation="RGAPEPYRMFTSRAEYRLRLRADNADQRLTDYGIEIGCVGVSRE
                     KLWREKSKKLSTAQVLAEGLQASPNELEAHGIMIKKDGQRRNVYDLLAYPDIDWVQLE
                     RVWPELKAIDLDIREQIECDALYANYMERHEADIAAFRKDEALALPKMLDYAEVGSLS
                     NEVRMKLEQARPATLGAASRIPGVTPAALVALLRHVKRKNSSQKLRRQSSRRGSETHV
                     A"
     gene            652..1272
                     /gene="rsmG"
                     /locus_tag="PK513_08985"
     CDS             652..1272
                     /gene="rsmG"
                     /locus_tag="PK513_08985"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOO82625.1"
                     /translation="MWPNSLQINVSRESMEKLEIYHALLLKWQKAVNLVSPKTLDEAW
                     QRHFADSAQLAGCVSRESSVLVDLGSGAGFPGLVLAIIRSELDVHLVESDERKCQFLR
                     TVSRETNVDITVHNARIESVKLGVIPDIVSARALADLKMLLDYCGPWIAVNPQLECLF
                     MKGAKARDEIIQAREFYDFECEVLPSKTDPAASILSLRDMSPKSRV"
     gene            1368..2222
                     /locus_tag="PK513_08990"
     CDS             1368..2222
                     /locus_tag="PK513_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO82626.1"
                     /translation="MKNLPARTVSPSFKTHDYPRLLAVANQKGGVGKTTTAINLATAL
                     SAVGKRVLIVDLDPQGNASTGLGVRRSLIKSSSYDVLFGDVAVEDAAVKTKVPRLSIV
                     PSSVHLSGAEIELVTAHSREYRLREALRRPLMGQGGPYDYVILDCPPSLNLLTLNALV
                     AVDSVIVPLQCEFYALEGLSHLTKTVERVRKQFNPRLDIQGVVLTMYDQRNNLTAMVE
                     RDVRQFFGDKVYKTVIPRNVRLSEAPSFGLPAIVYDMKCAGAQAYIHLASEVIKRERQ
                     IKKALLAA"
     gene            2275..3195
                     /locus_tag="PK513_08995"
     CDS             2275..3195
                     /locus_tag="PK513_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO82627.1"
                     /translation="MDPKSRGLGRGLNALFEDDEGVYPQVDPEGHIQGRKRDMVGIDQ
                     LEPGPGQPRTIFRDGALDELSESIKLHGVLQPLLVREHKDNSGQYQIIAGERRWRAAQ
                     KAQLHEVPVIVLSITDAEAFEIALIENLQREDLDPIDEATGYQKLIDEHEYTQEKLAE
                     SLGKSRSHIANMIRLLQLPLVVRDFLSDGKITIGHARALITVENAEALVKEVIAKNLS
                     VRETEKLVAESQGRAQTTKVRASKSSPAKDVDTLALEEDISGKLGMRVSIDSRDGKSG
                     RLSVQFKTLDQLDVLIRRLSEESGSGRLSA"
     gene            complement(3192..4088)
                     /locus_tag="PK513_09000"
     CDS             complement(3192..4088)
                     /locus_tag="PK513_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO82628.1"
                     /translation="MAQPLNDNRQYLKAAGFLLGSSFMIVLVNMFAKLLTENGLTPLE
                     VLFYRNLGGFALVTLTILSTRQFQRFKTKRPKAQFIRALTGNICLWLIIWANALLPLA
                     TVSAILLSAPIIATVLSPLFLNEKTSKFRGLCIAAGLTGALVITQPGMENFTPSYIVA
                     LMGALASAIVILYLRSLGQNEDPLTTTFYLFGWGSLFAGLCLPFFWTGLPSAYALLAG
                     IIISGFLSQFFKNNAYKHGEISFLSPLIYFNIVWATLLGWAVWKEIPEIHVYIGCAII
                     ICSNLLITWREQKMSQPHREVF"
     gene            complement(4091..5107)
                     /gene="holA"
                     /locus_tag="PK513_09005"
     CDS             complement(4091..5107)
                     /gene="holA"
                     /locus_tag="PK513_09005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO82629.1"
                     /translation="MKLAFRDIEPFVKAPNKAARVILVYGPDNGLMKERSATIGKTVV
                     TDLNDPFNVATLSTEIFLEDPARLPDEAGAISMMGGDRLIRIEGTSDKITTLIKEYLQ
                     TPSDSALVILEAGELGPRSTLRKLCESAKNAAAVPCYVEDERDLSRLIRETLTSSQIG
                     IDQDALTWLSANISGDRQKVRSELEKLITYKGNDNTPVTLDDVTACCGQTSAVALDDL
                     IYATAGHNTAQALKTYRQLMEEGVSFVVILRSLQNHFLRLHLIKSLIERGDSADSAMK
                     ALSPPIFFKQQHAFKTQVNRWSKEGLSKVLQKLMDLEAQCKQTGAPVETLCAQAVLGI
                     SKSR"
BASE COUNT         1217 a         1064 c         1582 g         1401 t
ORIGIN      
        1 cgcgcggtgc gccggagccg taccggatgt ttaccagccg tgcggaatat cgtttgcggt
       61 tacgggcaga taatgcggac caacgtttga cagattacgg tattgaaatc ggatgcgtgg
      121 gtgtttcacg tgaaaagctt tggcgagaga aatccaaaaa gctgtcaacg gcgcaggtgc
      181 tggccgaagg attacaggct agcccaaatg aacttgaggc gcatgggatt atgattaaaa
      241 aagacgggca gcgccgtaat gtttatgatt tgctggctta tccggatatt gattgggtgc
      301 agcttgagag agtgtggcct gagcttaagg ccattgatct ggatatccgt gagcagattg
      361 aatgtgatgc gctgtatgca aattatatgg agcggcatga ggctgatatt gcggcgtttc
      421 gtaaggatga ggctttggcg ttgcctaaga tgctggatta tgcagaagta ggcagtttgt
      481 caaatgaggt gcgcatgaaa ctggaacagg cccgcccggc gacgttaggt gcggcttcgc
      541 ggattcccgg cgtgacaccg gcggcgctgg tggcgttgct caggcatgtg aaacggaaga
      601 attcgtcaca gaaactcagg cgacaatctt cacgtcgggg gtcggagacc catgtggcct
      661 aattcgctac agatcaatgt ttcacgtgaa tccatggaga agctggagat ttatcatgcg
      721 cttttgctta aatggcaaaa ggcggttaat ctggtaagtc cgaagacgct ggatgaggcg
      781 tggcagcggc attttgcgga ttcggcgcag ttggccggat gtgtttcacg tgaatcatcg
      841 gttctggtgg atttggggag tggcgcaggt tttccgggtt tggtgctggc cataatccgg
      901 tcagaattgg atgttcatct ggttgagtcg gatgagcgta aatgtcagtt tttgaggacc
      961 gtttcacgtg aaacgaatgt ggatataaca gtgcataatg cgcgtataga atctgtaaaa
     1021 cttggggtaa ttccggatat tgtgagcgca cgggcgctag cggatcttaa aatgttgctg
     1081 gattattgcg gaccgtggat tgctgttaat ccgcagttgg aatgtttgtt tatgaagggt
     1141 gcgaaggcac gcgatgaaat aatacaagcg cgggaatttt atgattttga atgtgaggtt
     1201 ttgccgagta aaaccgatcc ggcggccagt attttaagtt tgcgtgatat gtctccaaaa
     1261 tcccgtgtat aagccttggt ggtatggcct gaaagcttga ccaacagcga gaatcgacgt
     1321 tattgtgtgt ctattccatt ttgtatcgat tcgagaggtt catgtccatg aaaaatcttc
     1381 ccgcccgcac tgtttcccct tctttcaaga ctcatgatta cccgcgtttg ctggcggtgg
     1441 cgaaccagaa aggcggggtc gggaaaacga caacggcgat caatttggcg acggcgcttt
     1501 cggcggtggg taagcgggtt ttgattgtag acctggaccc gcagggtaat gcttctaccg
     1561 gattaggcgt tcgccgttcg ttgattaaaa gcagctctta tgatgtgttg tttggggatg
     1621 tggcggtgga agatgcggcc gtgaagacca aagtgccgcg gttgtcgatt gttccgtctt
     1681 cagtgcattt gtctggtgcg gagattgagc ttgtcacggc gcatagccgt gaatatcgtt
     1741 tgagagaagc gctgcgccgc ccgttgatgg ggcaaggcgg gccttatgat tatgtgattc
     1801 tggactgccc accctcgttg aatttgttga cgctgaacgc gttggtggca gtggattcgg
     1861 tgatcgtgcc gttgcagtgt gagttttacg ctctggaggg gcttagtcat ctgaccaaga
     1921 ccgtcgagcg ggtgcgtaaa cagtttaatc cacggctgga tattcagggt gtcgttttga
     1981 cgatgtatga tcagcgtaat aatttgacgg cgatggttga gcgtgatgta cgccagtttt
     2041 ttggtgataa agtttataag actgttatac ctcgcaatgt gcgcttgtct gaggcgccgt
     2101 cctttggttt gccggcgatt gtttatgata tgaaatgtgc gggcgcgcag gcttatatcc
     2161 atttggccag cgaggtaatt aaacgcgagc gacagattaa aaaagcgttg cttgcagcgt
     2221 aaagcgattc tagagtttca cgtgaaacga tttctttttt gaaagggtta tggaatggat
     2281 ccaaaaagtc gtggtttggg gcgcggattg aatgccttgt ttgaagatga tgaaggtgtt
     2341 tatccacaag ttgatccaga agggcatatt caggggcgca agcgtgatat ggttggtatt
     2401 gatcagcttg agccggggcc gggacagccg cggactattt tcagagatgg cgcgctggat
     2461 gagctgagcg aatcgattaa attgcatggc gttttgcaac cattgctggt gcgagagcat
     2521 aaggataata gtgggcaata tcagattatt gccggtgagc ggcgttggcg ggcggcacaa
     2581 aaggcgcaat tgcatgaggt gccggtgatt gtgctgagta ttacggatgc tgaagcgttt
     2641 gaaattgcgt tgattgagaa tttgcagcgt gaggatcttg atcctattga tgaagcgact
     2701 ggctatcaaa agctcatcga tgaacacgaa tatacgcagg aaaaactggc ggaaagcctt
     2761 ggaaagagcc gttcgcatat tgcaaatatg attcggttgt tgcaattgcc gcttgtggtg
     2821 cgggattttt taagtgatgg aaaaataaca atcggccacg cgcgggcttt gattacggtc
     2881 gagaacgctg aggcgttggt caaggaagtg attgccaaaa atttgagcgt gcgtgaaacg
     2941 gaaaagcttg ttgccgaatc gcaagggcgg gcacagacta cgaaagtgcg ggcttcaaag
     3001 tcctctccgg caaaggatgt tgatactttg gccctggaag aagacatttc gggtaagctc
     3061 gggatgcgcg tatcgattga cagccgagac gggaaaagcg ggcggttgag cgtacagttc
     3121 aagacgcttg accagcttga cgttcttata cggcggcttt cggaagagag tggttccgga
     3181 aggctgtctg cctagaacac ctcccgatga ggttggctca ttttttgttc tctccatgtg
     3241 atcaggaggt ttgagcatat aatgatggcg cagccaatat agacgtggat ttccggtatt
     3301 tctttccaga ccgcccagcc caaaagcgtg gcccacacaa tgttgaaata gataagcggg
     3361 ctgagaaaag agatttctcc gtgtttatag gcgttgtttt taaagaactg gctaagaaag
     3421 ccgctgataa ttattccggc caataaagca taagcgcttg gcaggcccgt ccagaagaag
     3481 ggcaggcaaa gtcccgcaaa cagcgatccc cacccaaaca ggtaaaatgt cgtggtcagc
     3541 gggtcttcgt tttgtcccag gcttcgcaga tagagtatga caattgcgga ggccaaagca
     3601 cccatgagcg cgacgatata ggatggtgtg aaattttcca tgccgggctg cgttattaca
     3661 agcgcaccgg tcaggcctgc ggcgatgcat aatcctctga atttgcttgt tttttcgttc
     3721 aaaaagagcg gtgatagcac cgttgcgatg atgggtgcgc tcagcagaat tgcggatact
     3781 gttgccaaag ggagtagtgc gtttgcccat ataataagcc acaggcaaat gttaccggtg
     3841 agggccctta taaattgcgc tttgggacgt tttgttttaa agcgctggaa ttgtcttgtt
     3901 gataaaatgg tcagtgtgac gagcgcaaag cccccgagat tcctgtaaaa taaaacctcc
     3961 agcggcgtta atccgttttc tgtcaaaagc ttggcgaaca tgtttacgag gacgatcata
     4021 aagcttgagc ctaacaggaa cccggcggcc tttagatatt gacggttatc gtttaacggt
     4081 tgcgccatgt ttagcgggat ttgctgatgc cgagcacggc ctgggcgcag agggtttcca
     4141 cgggcgcgcc ggtttgtttg cattgggcct ctaaatccat aagcttttgt aatactttgg
     4201 ataatccttc tttagaccag cggtttactt gcgttttaaa ggcgtgttgc tgcttgaaaa
     4261 aaattggcgg gcttaaggct ttcatagctg aatctgccga atctcccctt tcaataaggc
     4321 ttttgattaa atgcaggcgc aggaagtgat tttgcaggct gcggaggatc acaacaaaag
     4381 acaccccctc ctccatcaat tgacgatagg ttttaagcgc ttgggcagta ttatgcccag
     4441 cggtagcata gataaggtca tcgagtgcga cagcgctggt ctgaccgcaa catgcggtga
     4501 catcgtctaa tgttacaggg gtgttatcgt tgcctttgta ggtaatgagt ttttcgagtt
     4561 cgctgcggac tttttggcga tcaccggaga tattggcgct gagccaggtg agtgcgtcct
     4621 ggtcgatgcc gatttgggag cttgttaatg tttcgcggat caggcgagac agatcgcgct
     4681 catcttctac gtaacaaggc acggcggcag cgttttttgc tgattcgcag agtttacgaa
     4741 gggtgctacg ggggccgagt tcaccggctt cgaggattac cagcgctgaa tctgaggggg
     4801 tttgcagata ttccttgatt agtgtggtga ttttgtcgct ggtaccttcg atccggatca
     4861 ggcgatcgcc gcccatcatc gagattgccc cggcctcgtc aggcaggcgg gccgggtctt
     4921 cgaggaaaat ttcggtggat agcgtggcga cattaaacgg gtcgttcagg tctgtaacga
     4981 cggttttgcc aatggtggcg ctgcgctctt tcattaggcc gttgtccggg ccgtagacga
     5041 ggattaccct tgcggccttg tttggcgctt tgacgaaagg ttcgatgtcg cgaaaagcca
     5101 gtttcatatt cgagatccta tagcatgtgc cgggcgggtc aattgttctt acattgatgg
     5161 ccataaacta taaacatacc gactggtcgg tatgtttatg cttgttgtta tttataggca
     5221 cgtaaaaacc aaggggcttg ccccgtggat ggagtgcgtc cagt
//
LOCUS       DAOSWR010000135         5243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10531_length_5243_cov_7.633385, whole genome shotgun sequence.
ACCESSION   DAOSWR010000135 DAOSWR010000000
VERSION     DAOSWR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10531_length_5243_cov_7.633385"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..464)
                     /locus_tag="PK513_09010"
     CDS             complement(111..464)
                     /locus_tag="PK513_09010"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOO82630.1"
                     /translation="MRHFYLSDTQWEAIKPHLPPDRGRKPRVDDRRVISGIIHVLQSG
                     CRWRDAPSEYGSYTTLYNRYNRWSARGIWQYLFREIVGALSDERDMAAIDSTHIKAHR
                     SAGGGKGGRMLRRSA"
     gene            complement(719..1663)
                     /locus_tag="PK513_09015"
     CDS             complement(719..1663)
                     /locus_tag="PK513_09015"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HOO82631.1"
                     /translation="MSSSISLSTKIAGSTLSSCLYNASGPLCSTAEELHAIGKSHAGA
                     ILSKSATLQERQGNEEPRYVDTRWGSINSMGLPNKGFEFYAQFAQTAKVYEKPYFMSV
                     SGLSHEDNRRIIKELANFENIAAIELNLSCPNVPGKPQTGYDFEQSRHLLELVFTAAK
                     QPIGVKLPPYFDMPHFEMMAAVLNDFPISFVTCINSIGNGLVIDPDSESVVIKPKHGF
                     GGIGGDFIKPTALANVRKFYTLLDKKVSIIGCGGVKTGPDAFEHILCGASAVQIGTQF
                     MREGTGCFERIANELTDLMRQKGYQNIESFKGKLRDIT"
     gene            1807..2190
                     /locus_tag="PK513_09020"
     CDS             1807..2190
                     /locus_tag="PK513_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82632.1"
                     /translation="MHKNNSKHFCLNVFIILGLVLSNLAPLCANKASAKIFLEICTAN
                     GLQVIALNGDAPDDGDPAPGDKPNRKMCSYCVASHMARVIPDLPDALHSQIRRTNISI
                     VSYSDDLTLLLQAKRYEARAPPYAA"
     gene            2260..4347
                     /locus_tag="PK513_09025"
     CDS             2260..4347
                     /locus_tag="PK513_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOO82633.1"
                     /translation="MHNIFKYSAFGASLLVSSFLVAPSYAEEIIEVDPIAVEALMMMS
                     YPASASYDSSDAASPLRYSDGADYLGSLPGVTAARFGGHGLEPFIRGQSQGQLNIVNG
                     DSYVFGGCPNRMDPPSAYMNIQLRDTVTVIQGYQSVLNGFGGSGGSVIVEQGRPDFGE
                     ALAVTGLVQGGYDGNSEMWNAGANVTAGTLLGYANAYGSYKDAENYEDGDGDEVRAAF
                     IERSGGLKVGYTPEGAHLSLGADYRKINDALFPGAGMDSPLSKGQTFKAALERDLDGK
                     IFQRVKVSTYASLVDHIMDNFSLRPFTAAMPLRVDSQSDTYGAKLEKDFVLFDQPVES
                     VLEWRRNNRNADRIVNTTGALQSLLWPDITINEASLAGESAYDLGDKSRLVVGGRYDY
                     VHVDYGRADTPAPAAGNRTPNDIYSQFYGYGASSKTEHNLGGLLRLEHDYSASTLFYT
                     GLSRSVRTADATERGLANFMGAGGATSWVGNPDIDPEKHHQMDIGFDITEPVWSFGGS
                     AYANYVEDYILRDSARSQPGILVNFPDADIYRNINAFLAGFEVRGFWRFASDWTLEAD
                     ATCTFGADIDAGRALPQIPPLQGKVGVTWQALDYLEVSGKARWALEQTRVDTNSLSST
                     GRDVDKTNGYAVFDLEGTITEFDPASLNFGVKNIFDSTYANHLNRSNISDPTEVQVNE
                     PGRSFYVQLRVPF"
     gene            4521..5072
                     /locus_tag="PK513_09030"
     CDS             4521..5072
                     /locus_tag="PK513_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOO82634.1"
                     /translation="MEYAPIFVTLVQEPSPLPQTPEKIESPALQNDRRDDVQKALDLT
                     LADPVPAPTEKNEKKRESSSITRTAAQASSMRRQNSAYAGGGRNAEIRYQDMVRAAID
                     SYRVYPREARRRKLEGHAVIQIRISRSGAITESRFVKTTGHHILDQAALNMVKAASPL
                     PAIPSSLGKSSVSMNIPIGFKLK"
BASE COUNT         1296 a         1196 c         1321 g         1430 t
ORIGIN      
        1 ggcaaccgcc cctttgatat cggcgcgttg cccttgcgtg atgtacaaag ccagcggacg
       61 gcatttcata tcgctgatcg cgtacagctt ggtgttgcgt ccgccacggc ttatgccgat
      121 cgcctgagca tgcgcccccc ctttccgccg ccggctgatc tgtgcgcttt gatatgcgtg
      181 ctgtcaatcg ccgccatgtc ccgttcgtcc gataacgcgc caacaatctc gcggaacaga
      241 tattgccaga tgccgcgcgc agaccagcga ttataacgat tataaagggt ggtgtaagaa
      301 ccgtattcgg acggcgcatc acgccagcgg caacccgatt gcaaaacatg aatgatgccg
      361 ctgatcacac ggcggtcatc cacgcggggc ttgcgtcccc tatctggcgg caagtgaggc
      421 ttgatcgctt cccattgggt gtctgaaaga taaaaatgac gcatagtttg gctcctttct
      481 caaatcacca aaccattgaa tcataaaatt acctgtgtgt ttagacccta gtctcagatg
      541 acagatattt taaaaccgcc aacgaaacag aactcgaagc cataaaaatt ccccccgagc
      601 attttagtca taatattact caagccgcta aagttcttgg catggcaaaa tctacatttt
      661 ataagaatat gaaggcccga tatttgccaa tttctgtcca aatttgcccg cgcgcatatc
      721 atgttatgtc tcttaatttt cccttaaagc tctctatatt ttggtagcct ttttgccgca
      781 taaggtcggt gagttcattg gctatgcgtt cgaagcatcc tgtcccttcg cgcataaatt
      841 gtgtgcctat ttgcacagcg cttgcgccac ataaaatatg ctcgaaagca tccgggccgg
      901 tttttacgcc gccacagcca ataatagata cttttttatc aagcaaagta taaaatttac
      961 ggacattggc aagggctgtt ggtttgatga agtctcctcc tatgcctcca aatccgtgct
     1021 ttggtttaat aacgacagat tctgaatccg ggtcgatcac cagaccattg ccgatactgt
     1081 taatacatgt gacaaaagag atgggaaaat cgtttaatac ggcggccatc atttcaaaat
     1141 gcggcatatc gaaatatggt ggaagcttca cgccgatagg ctgttttgct gctgtaaaaa
     1201 ccaattcgag aagatgacgg ctctgttcaa aatcatatcc tgtttgcggt tttccgggaa
     1261 cattggggca tgaaaggttt aattcaatgg cggcaatgtt ctcgaaatta gccagctctt
     1321 taataatgcg gcggttatct tcatggctta gtcctgagac agacataaaa tagggtttct
     1381 cgtaaacttt cgcagtttgt gcaaattgag cgtaaaactc aaatccttta ttgggcagtc
     1441 ccatgctgtt aatactgccc cagcgcgtgt caacatatcg gggttcttca tttccctgcc
     1501 gctcctgcag ggttgcgctc ttgctcaaaa ttgcgcccgc atgacttttt ccaatagcgt
     1561 gtaattcctc tgcggtggag cataaaggtc ctgaagcgtt atacaggcac gaggaaagag
     1621 tggaaccggc tatttttgtg gaaagggaaa ttgatgaaga catgtggtta ttctttcaga
     1681 tgagacggat gaattcacat atctattgat agtaaactca ttatagaaga gcaacgagaa
     1741 aagttttatg tgttttcttt gtttggaatg gtttagaaat tgcagagaag tagcaggttt
     1801 agatctttgc ataaaaataa cagcaaacat ttttgtttaa acgtttttat tattctgggg
     1861 cttgttttat caaacctcgc gcctctttgc gccaataaag catctgcgaa aatatttctg
     1921 gaaatttgca cggcaaacgg tcttcaagtt attgccctta acggggatgc accggatgac
     1981 ggcgatcccg cgcctggtga taaaccaaac aggaaaatgt gctcttactg tgttgcttct
     2041 catatggcgc gggttattcc tgatctgccc gatgcgcttc actcgcaaat tcgtcgtact
     2101 aacatatcca tcgtctccta tagcgatgat ttgaccttat tgcttcaggc aaaacgttat
     2161 gaagctcgcg caccccctta cgccgcttaa gacccatttt taatattctt aaagccatcg
     2221 cacagttggt ctgtgcgctt atatgtgtaa ggaaaaacaa tgcataacat tttcaaatac
     2281 agcgcctttg gcgcgtccct gcttgtcagc agttttttag ttgctccgtc ttatgcggaa
     2341 gaaatcattg aggttgatcc gattgcggtt gaggccctca tgatgatgtc ttatccggcc
     2401 agtgcttctt atgatagttc tgatgcggca tctcccctgc gctattcaga tggggcagat
     2461 tatctgggat ctttgccggg ggttacagcg gcacgttttg gcggacatgg gcttgagcct
     2521 tttattcgag ggcagtcaca agggcagttg aatatcgtca atggcgattc ctatgtattt
     2581 ggagggtgcc caaaccgtat ggacccgccg agcgcttata tgaacataca gctccgcgac
     2641 accgtaacag ttattcaggg ctatcagtct gtgctcaacg gattcggagg ctctggcggc
     2701 agtgtgatcg tcgaacaagg gcggcctgat tttggtgaag ctctggcggt cactgggctt
     2761 gtgcagggcg gttatgacgg caatagcgaa atgtggaatg ccggggccaa cgttacggca
     2821 ggcacgttac ttggctatgc gaacgcctat ggttcttata aggatgctga aaattatgaa
     2881 gacggcgatg gtgatgaagt acgcgccgct ttcatagagc gctcgggcgg cctgaaagtt
     2941 ggttatacac cggaaggggc gcatctatct ctgggcgcag attaccgcaa gatcaacgac
     3001 gctctgtttc ccggcgcggg catggactcg cccttatcca aagggcaaac attcaaggcc
     3061 gctttggaac gagatctgga tggaaagatt ttccaaagag tgaaagtcag cacctatgcg
     3121 tctttggtag atcacatcat ggataatttt agtttgcgtc cgtttacagc ggccatgcct
     3181 ttacgtgttg attcgcagtc cgacacgtat ggtgcaaagc tggaaaaaga ttttgtcctt
     3241 tttgaccagc ccgttgagag cgtgctggaa tggcggcgga ataaccgtaa tgccgacagg
     3301 attgtgaaca caacgggcgc tcttcaatca ctgttatggc cggatattac gatcaatgaa
     3361 gccagtcttg ccggtgagag cgcttacgac cttggtgata agagccgttt ggtcgtgggt
     3421 gggcgttacg attatgtgca tgtcgattat gggcgtgctg atactcctgc cccggcggca
     3481 ggaaatagaa cgccgaatga tatttattct cagttctatg gctacggcgc gtcatcaaaa
     3541 acagagcata atctgggcgg ccttctgcgt ctggaacatg attatagtgc tagcacgctg
     3601 ttttacaccg gtttgagccg ctccgttcgc acggcggatg caacggaaag aggtcttgcg
     3661 aactttatgg gcgcaggtgg agcaacttcc tgggttggaa atcctgacat tgatccggaa
     3721 aaacatcatc aaatggatat aggttttgat attacggaac ctgtctggag ttttggtgga
     3781 tcagcctatg ccaattatgt ggaggattat atcttgcgtg attctgctcg ttctcaaccg
     3841 ggtattttgg tgaattttcc tgatgctgac atataccgca atattaatgc ctttctggcg
     3901 gggtttgaag ttcgtggatt ctggcggttt gcttcggact ggacgcttga ggcagatgcg
     3961 acttgtacgt ttggcgcgga tattgatgcc ggacgggctt tgccacaaat tccgcctttg
     4021 caaggtaaag taggcgtaac atggcaagct ttggattatc tggaggttag tgggaaggcg
     4081 cgctgggcgc tggagcaaac gcgagttgat acaaattcgt taagctcaac aggccgggat
     4141 gttgataaaa ccaatggtta tgccgttttt gatttggaag gaacgatcac cgaatttgat
     4201 cctgcgtcgc tcaattttgg ggtgaaaaat atatttgatt ccacttatgc caatcacctt
     4261 aatcgctcca atatttccga cccaacggaa gtgcaggtga acgaaccggg ccggagtttt
     4321 tatgttcaac tgagagttcc gttttgatgg tggctttgaa aacccataaa cgcctgtctt
     4381 ccgctatacc gtcttttaaa accagtatag cggggacagc tttatcgttt tgtgcgtcta
     4441 ccgtgttgca tatgagcgtc atcgggggga tcgtggcact ctcctcgtat atccatgacg
     4501 ctggcggagg agccgggaaa atggagtatg cacccatttt tgtaactctc gttcaagagc
     4561 cttcgcctct tcctcaaaca cctgaaaaga ttgaaagtcc cgctttgcaa aatgaccgta
     4621 gggatgatgt gcaaaaggca cttgatttaa cgcttgccga tcctgttcca gcgccaaccg
     4681 aaaaaaatga aaaaaagagg gaaagctcct cgattaccag gacagcagct caagcatctt
     4741 caatgcgcag acaaaacagc gcgtatgcag gtggtggaag aaacgcagaa attcgctatc
     4801 aagatatggt gcgtgcggca atagattctt atcgcgtcta tccacgcgag gcccggcggc
     4861 gaaaactcga ggggcacgcg gtgattcaaa ttcgtatcag ccgaagcgga gccatcactg
     4921 aaagccgttt tgtaaaaaca acagggcatc atattcttga tcaggccgct ttaaatatgg
     4981 tcaaggccgc cagcccctta cccgctatac cttcttcctt aggcaagtct tctgtctcaa
     5041 tgaacattcc tattggtttt aagcttaaat aatcgtacct gtttgtgata agttaggagc
     5101 gcttaaggac gtaaggcgac actccccatt ggtcactact ccaccgtgac ggatttggcc
     5161 agattgcgcg gttggtcaac atcggtgcct tttgtaacgg caacgtggta agcgagaagc
     5221 tggatcggta cggcatagag aat
//
LOCUS       DAOSWR010000136         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10568_length_5229_cov_5.848860, whole genome shotgun sequence.
ACCESSION   DAOSWR010000136 DAOSWR010000000
VERSION     DAOSWR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10568_length_5229_cov_5.848860"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1473)
                     /locus_tag="PK513_09035"
     CDS             complement(52..1473)
                     /locus_tag="PK513_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82635.1"
                     /translation="MSEVNASDTRRIESIDDVIDTYAGVGADAKAGIEVELAFFDPES
                     ADLAPMTIAQNKVAKNTTNAEYDGDFVRNEPTSEMLEVGSIARGPKDLRMIMDDTNKK
                     IVCLTQKAATIGLKRSYFQHLPNKSAADLLNNVMDIERYQAFFAPPRKDMQAIAAYFS
                     VCKSNQVSISYRSPGHLLENVRRLYCLAPFLFMITDNAAPFNEGHAFTGHVGMHHRTS
                     LKDRGGVPPYIYSAKTGDDYIHGHGDHVINNPLFVYYDEHGKLIRLPSGTWTSFNKLR
                     EQGLNTATNYFFAESILWPDIKIAALKDENNQVVNHRYEARMFGVGLHQHQTALLLTA
                     TLAFDPEFAKQVDILLARSGLNQMQPKTLQKPLEAAYKAAQNHNGRFLNVPYGNTTML
                     AFAQKFATLIENSTYLEGLEDELGPLLTICHTGCTDSKVNALLFNTLESALTFQRDYD
                     PVIFKNPNLCAYTLFEKQLKKLK"
     gene            complement(1604..1891)
                     /locus_tag="PK513_09040"
     CDS             complement(1604..1891)
                     /locus_tag="PK513_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82636.1"
                     /translation="MLIVPFRNDNRETIEIRYVVPDGRPGPAALVPLTTISREVFNDY
                     GQDEPVEAAFEKAVTAVLNKTSLPKAHDELQGLANRSVQEHAEELLTIINH"
     gene            complement(1931..2944)
                     /locus_tag="PK513_09045"
     CDS             complement(1931..2944)
                     /locus_tag="PK513_09045"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOO82637.1"
                     /translation="MAVILPDGHPAIENLRQEGCKNVIPASQAPKDNVVRVAVANTMS
                     NVVETETQFARAFAESGLNVELVFYSPSTKIAAAHEKSDSKKEQHRLKYHKPLSEIRN
                     DKTINGIVLTGFPGANLDLRQKTGSTDDKGNEFYNEFIDLLDYARKSNTPTLATCWAA
                     HVALNHFYGIERLKREAKLTGIFELAVTNANSPLVKGIEHKFPMPVSRYYYSDEIALR
                     AQPNLNILASSEETGAAIVSEKKGPFIYMSGHLEYLANTLLKELLRDFQKKGTDAPYP
                     ENLDPFYQKRTWAAANTAFYSNLANLFNASRTNKLAAARILQPHRTDDWSYTTQEPAA
                     VYR"
     gene            complement(3294..3824)
                     /gene="lptE"
                     /locus_tag="PK513_09050"
     CDS             complement(3294..3824)
                     /gene="lptE"
                     /locus_tag="PK513_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOO82638.1"
                     /translation="MQVTSYKIKIALFLVFATCTLQLTSCGLHPVYGVNKYTAVGVEE
                     HLAAIEIGGIPDREGQFLRNALIDRFYRAGRPVNPLYKLTVSPVSESRRDLDVTIDSD
                     TTRGQLTLKTNISLIDKQTGKVLLQRPLRSIASYNILASEFANRVSEQNTRENALNGL
                     ARQIEEQLALHFKHAQ"
     gene            complement(3835..>5229)
                     /locus_tag="PK513_09055"
     CDS             complement(3835..>5229)
                     /locus_tag="PK513_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO82639.1"
                     /translation="NSQWLDGMSVEDAKAEVIKRCENAGTGFGTTQFRLRDWGISRQR
                     YWGCPIPIIYCEDCGAVPVPEDQLPVTLPDDISFNKPGNPLAHHPTWKNCACPKCGKD
                     AQRETDTFDTFFESSWYQFRYCDPHNETAPVDKDKAKYWMPVEQYIGGVEHAVLHLLY
                     ARFFTKAMRDCGYVDCDEPFTGLFTQGMVTHETYQDANGKWLFPSEYEALTDKTKAKI
                     GPSIKMSKSKKNVIDPQDIIDTYGADAARLFILSDSPPERDLEWTEAGIEGAWKFINK
                     LHRTLMEARTSLPGADSATPLDFSDNATNLRRASHKTIVGVAKAIEDFAMNKAVAQIR
                     ELSNMINGFKPGNDSDRWALREAFETLTQLFNPMMPHLAEELWAQLGHSSLLADEPWP
                     KADKTLLTDDTVTLGVQVNGKVRAQINLAPDADEATARETALSQDNVKKFIEGKQVRK
                     FIYVPGKIVNVVAA"
BASE COUNT         1121 a         1112 c         1501 g         1495 t
ORIGIN      
        1 acaagcgcag cttgaactaa agtgatacca cgggccttgg cccgtggagt ttcacttcag
       61 ttttttgagt tgtttttcaa acagcgtata ggcgcagagg ttcgggtttt taaaaatgac
      121 agggtcgtaa tcgcgttgaa aagtcagggc cgattcgagc gtattgaaaa gtagcgcatt
      181 gactttggaa tcagtgcaac cggtatggca aatggtgaga agggggccga gttcgtcttc
      241 aaggccttcc aaatatgtag aattttctat aagcgttgca aatttttgtg cgaaagccag
      301 cattgtggtg ttaccgtagg gaacattgag gaatcgaccg ttatggtttt gggcggcttt
      361 gtatgcagct tctagcggtt tttgaagcgt tttgggttgc atttggttca agccggagcg
      421 tgcgagcaaa atatcgactt gtttggcaaa ttcagggtca aaggccagcg tggcggtgag
      481 cagcagtgca gtttggtgct ggtgcaggcc gacaccaaac atgcgggctt catagcggtg
      541 gttgactacc tgattatttt cgtctttcag ggcggcgatt tttatatccg gccagagaat
      601 actttcggcg aaaaagtaat tggtggctgt gtttaggccc tgttcacgca atttattaaa
      661 gctggtccat gtaccggagg gcaggcggat aagtttgccg tgttcgtcgt aatagacaaa
      721 aagcgggttg tttatgacat ggtcgccatg gccgtgaata taatcatcgc cggttttggc
      781 gctgtatata tagggcggga cgccgccgcg gtcttttaat gaggtgcggt gatgcatgcc
      841 gacgtgccct gtaaaagcgt ggccttcatt gaatggtgcg gcattgtcgg taatcataaa
      901 taaaaagggc gcgaggcaat aaaggcggcg gacattttcg agcagatgtc cggggctccg
      961 atagctgatc gagacttgat tggatttgca gaccgagaaa taggcggcaa tggcttgcat
     1021 gtccttgcgc ggcggggcga aaaaggcctg atagcgttcg atatccatga cgttattgag
     1081 caggtcggca gcgcttttgt tgggcaggtg ctggaaataa gagcgtttaa ggccgatagt
     1141 agcggctttt tgcgtgaggc agacgatttt tttgttggta tcgtccatga tcatgcggag
     1201 atctttgggg ccacgggcaa tcgagccgac ttcgagcatt tcggatgtgg gctcattgcg
     1261 gacgaaatcg ccgtcgtact ccgcgtttgt ggtgtttttg gcgaccttgt tttgcgcaat
     1321 tgtcatcggg gcgagatcag cgctttcggg gtcgaagaag gcgagttcaa cttcgatgcc
     1381 ggctttggca tccgcgccta cgcccgcata ggtgtcgatg acatcgtcga tcgattcgat
     1441 gcgccgcgtg tcgctggcat ttacttcgga catatgtcgg gcccctttct ccttacttac
     1501 cttgcgtaat atgcggtagt tcaggttgaa aggcaaattt gctggataaa taaaaacgcc
     1561 agcacaaaga gggtgccagc gtttttaggg tttctccatt ttatcagtgg ttgatgatgg
     1621 taagaagctc ttctgcgtgt tcttgaacgc ttctgttggc gagtccttgt aactcatcgt
     1681 gtgctttggg caggcttgtt ttattaagca cagccgtaac cgctttttca aaggcggctt
     1741 ctacgggttc gtcttgacca taatcgttaa atacttctcg acttattgtt gtaagaggaa
     1801 cgagggctgc ggggccgggt cttccgtccg gtacaacata gcgaatttcg attgtttctc
     1861 gattatcatt tctaaaggga actatcagca ttttcttttc ctttcgttgt aaaatatttt
     1921 tgcagatgtt ttagcggtaa acagcggcgg gttcttgtgt tgtataagac cagtcgtccg
     1981 tgcggtgggg ttggagtatt cgcgcagcgg ccagcttatt cgtacggctt gcgttaaaaa
     2041 gattcgccag attgctatag aaggcggtgt ttgccgcagc ccatgtgcgt ttctgataaa
     2101 aagggtcaag attttcgggg tagggtgcat ccgtgccttt cttttgaaaa tctcttagca
     2161 attcttttag taaagtgttg gctaaatatt cgaggtgacc gctcatatag ataaaaggcc
     2221 cctttttttc tgaaacaatc gctgcgccgg tttcttccga acttgctaaa atgttcaggt
     2281 ttggctgggc tcgcagtgcg atttcatcgg agtagtagta acgtgaaacg ggcatgggga
     2341 atttatgttc tatgccttta accagtggcg aattcgcatt ggttacggcc agttcgaaga
     2401 tgccggtaag tttagcctca cgttttagtc tttcgatgcc gtagaaatgg ttgagcgcga
     2461 cgtgagcggc ccagcatgtt gccagcgtcg gcgtattgct tttacgggca taatcaagca
     2521 ggtctataaa ttcattgtag aattcattgc ctttgtcgtc tgtggagcct gttttttgtc
     2581 tgaggtctaa gtttgctccc gggaaacctg tcaggacaat tccgttaatg gtcttgtcgt
     2641 ttcgaatttc agagagcggt ttgtggtatt ttaaacggtg ctgttctttt ttactgtctg
     2701 atttttcgtg ggcagcggcg atttttgttg aggggctgta aaagacaagc tcaacattca
     2761 ggccgctttc ggcaaaggcg cgggcgaact gcgtttccgt ttccacaacg ttggacatgg
     2821 tgttggccac ggccacacgc acgacattgt ctttaggggc ttgcgacgcc gggataacgt
     2881 ttttgcagcc ttcttgtcgc aaattctcaa ttgccgggtg tccatctgga agaataacag
     2941 ccatgtttta aagtccattt ccttaaatat tggctgtgct gataacagag cctgatagcg
     3001 gaaaaccgaa atcaaaaaag ccatgcgtgt tagcacagtg gttaaataca atatctgtcg
     3061 ggaatgggtg gacgtttgtc cgcggtcatg ccccgccagg tttccaaaat tcaggagacc
     3121 acgggcggga tcaaaatagc gttggtttga tctttgccgt ggcgctttag cttttttaac
     3181 gcggtgcaag ctgagtccat caaatgccgc ggaagtgcta taagccatcg tttttgctta
     3241 tagaccagat ataaaacgat cgtcaagata tttttctgta aagttatgtg cgtttattgt
     3301 gcgtgtttga aatgcagggc gagctgttct tcaatttggc gggcgaggcc gttcagcgcg
     3361 ttttcacggg tgttttgttc cgagacgcga ttggcaaatt cgctggccag aatattgtag
     3421 ctggcgatgg atcgcaaagg gcgttgcagg aggactttgc cagtttgctt atcaatgaga
     3481 gagatattgg ttttaagggt taattgtccg cgggtggtat cggaatctat agtgacatcg
     3541 agatcgcgtc ggctttccga gacgggtgag accgttagct tgtaaagcgg gtttaccggg
     3601 cggccagcgc gatagaaccg gtcgatcaat gcattgcgca gaaactggcc ttcgcgatca
     3661 gggatacctc cgatttcaat tgcagcgaga tgttcttcga cgccgacagc ggtgtattta
     3721 tttacgccat agacagggtg gaggccgcag gatgttagtt gcaaggtaca agttgcaaag
     3781 accaagaaca gcgcgatttt tatcttgtaa cttgtaactt gcatcttgta cctcctaagc
     3841 tgccaccacg ttgacgattt tgccgggcac gtagatgaat ttgcgcacct gtttgccttc
     3901 gatgaatttt ttgacattat cttgcgacag cgcggtttcg cgggcggtgg cttcatcggc
     3961 gtctggagcg aggttaattt gcgcgcggac cttgccgttg acctgtacgc cgagggtgac
     4021 agtgtcatcg gtcagaagcg ttttatcggc tttgggccat ggttcatcag ccagcaggct
     4081 agagtgacct aattgtgccc atagctcttc ggctaggtgc ggcatcatcg ggttgaaaag
     4141 ctgagtaaga gtttcgaatg cttcgcgcaa agcccagcgg tctgaatcat tcccgggttt
     4201 aaagccattg atcatattag acagttcacg gatttgcgct acggccttgt tcattgcaaa
     4261 atcttcaatg gctttggcga cgccgacgat tgttttgtgt gatgcgcggc gtagattagt
     4321 cgcgttgtct gagaaatcaa gcggcgttgc actatctgca ccgggcagac tggttcttgc
     4381 ttccattaat gtgcggtgaa gtttattgat aaatttccag gcaccttcga tgccggcctc
     4441 cgtccattcc agatcacgtt cgggcgggga gtcagagagg atgaatagcc gtgccgcatc
     4501 cgcgccgtaa gtgtcgataa tgtcctgcgg gtcgatgacg ttctttttcg atttggacat
     4561 tttgatcgat gggccgattt tggctttggt tttatcggtc agagcttcat attcggatgg
     4621 gaagagccat ttgccattgg cgtcctgata agtttcgtgc gtgaccatgc cttgtgtgaa
     4681 caggcctgtg aatggctcat cgcaatcgac gtagccgcag tcgcgcatgg ctttggtgaa
     4741 aaagcgggcg tagagcagat gaagcacggc gtgttcgacg ccgccgatat attgctcgac
     4801 cggcatccag tatttggctt tgtctttatc aacaggcgcg gtttcattat gcgggtcgca
     4861 atagcggaac tgataccacg agctttcaaa gaaagtatca aacgtgtcgg tttcgcgctg
     4921 ggcatcttta ccgcatttcg gacatgcgca gtttttccat gtcgggtggt gggcaagcgg
     4981 gttgccgggt ttgttaaagc ttatatcatc ggggagtgtc acgggaagtt ggtcttctgg
     5041 aaccgggacc gcgccgcaat cctcgcaata aatgatgggg atcgggcagc cccaatagcg
     5101 ctggcgggag atgccccaat cgcgcaggcg gaactgcgtg gtgccaaaac cggtgcctgc
     5161 gttttcgcag cgcttgatga cttccgcttt ggcatcttcg acgctcattc catcgagcca
     5221 ctgggaatt
//
LOCUS       DAOSWR010000137         5223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10582_length_5223_cov_5.176084, whole genome shotgun sequence.
ACCESSION   DAOSWR010000137 DAOSWR010000000
VERSION     DAOSWR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_5223_cov_5.176084"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK513_09060"
     CDS             <1..252
                     /locus_tag="PK513_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO82640.1"
                     /translation="YEPVWAIGTGKTASVDDVVEMHGLIREQLQELVPNADKVRILYG
                     GSMKPENAKELLSTENVDGGLIGGASLSAESFLAIARAA"
     gene            273..623
                     /gene="secG"
                     /locus_tag="PK513_09065"
     CDS             273..623
                     /gene="secG"
                     /locus_tag="PK513_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOO82641.1"
                     /translation="MESVLLVIHLFLALAIIGLVLLQRSEGGGLGIGGGSGGLGGLAT
                     PQGTASLLTRATAICAAAFFCTSLILGVLAGRSTSQQVSLMDALSETPASIEAPLDEV
                     EQKPEEAPSVPIAD"
     gene            626..2260
                     /locus_tag="PK513_09070"
     CDS             626..2260
                     /locus_tag="PK513_09070"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOO82642.1"
                     /translation="MTRYIFITGGVVSSLGKGLGSAALGALLQARGYKVRLCKLDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTGVPARKNDNITTGQVYTEVLGKE
                     RRGDYLGATIQVIPHITNEIKDFILEKEGTADFVLCEIGGTVGDIESLPFLEAIRQFG
                     NEVGHDRALYVHVTLLPYIPAAGELKTKPTQHSVKELMSYGIRPDILLCRADREIHQE
                     ERRKIALFCNLAEKNVIPALDVSSIYEVPLSYHAEGLDHQVMECFGIKDIANLDLSIW
                     EQIVRRIKEPESTVKIAVVGKYTNLADSYKSLNEALIHGGVANKVKVKLKFIEGEDFE
                     GGVNLAEHLQGVHGILVPGGFGKRGTEGKIKAVEYARKHNIPYFGICLGMQMAIIEAA
                     RNLCGIEDATSSELSDGGTHVIGLMTEWETEEGGKEQRSEDTDLGGTMRLGAYPAKLV
                     EGSRVAEIYGSEEISERHRHRYEVNINYREQLEDQGLVFSGLSPDGELPEIVERTDHP
                     WFVGVQFHPELKSKPFDPHPLFVSFIEAALKQSRLV"
     gene            2262..2705
                     /locus_tag="PK513_09075"
     CDS             2262..2705
                     /locus_tag="PK513_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOO82643.1"
                     /translation="MKAVLDTNIFVGALQRRDGVNRAVLKQAFLDDITPLMSEALYSE
                     YQSLMGRAHLFEDSSFNAQECEDFFDDFCSICRWVDVYYRWRPNLHDEADNHVLELAI
                     SGGAETLITWNKKDFRHGNLVLPEIAILTPVEYFRMQREQSVKNH"
     gene            2756..2962
                     /locus_tag="PK513_09080"
                     /pseudo
     CDS             2756..2962
                     /locus_tag="PK513_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007303342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
     gene            complement(3068..3763)
                     /locus_tag="PK513_09085"
     CDS             complement(3068..3763)
                     /locus_tag="PK513_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82644.1"
                     /translation="MARMKDIRAAQRAAATGNPTQTKVSPKKLSKEQNQRRIHGNQGE
                     TPEMHQIRKILRKERQNFNDYQAICKKATSDQHSSITAQDINNYTDGADMKSHHIPPL
                     KHALQELGLFKAQTKPQQEGKKTSKQENGLNGEGISDNFWTTVKVDNTYPNNGDINLD
                     RIAAVSEALHTATGIHVNKLKTLLVQGIEMTKALEANASKHNNESKIPAITVRLRPDQ
                     QAALVDAYNTLDI"
     gene            3892..4713
                     /gene="kdsA"
                     /locus_tag="PK513_09090"
     CDS             3892..4713
                     /gene="kdsA"
                     /locus_tag="PK513_09090"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOO82645.1"
                     /translation="MKTVSVDKLQIANDRSFVLIAGPCQMESRDHAFDMCGALVEITK
                     NLDIPFIYKSSFDKANRTSLHGVRGMGLDGALPVFADLKREFGVPVLTDIHTAEQCAD
                     VAEVVDVLQIPAFLCRQTDLLVTAAKTGAVINVKKGQFLAPWDMKNVAAKISESGNDQ
                     ILLTDRGTSFGYNTLVSDMRGLPIMARTGYPVIIDATHSVQQPGGQGASSGGDREMVP
                     VIARAAVAVGVAGVFIETHQDPDNAPSDGPNMVKLEDMRGLLEILMKLDKITKNA"
     gene            4777..5175
                     /locus_tag="PK513_09095"
     CDS             4777..5175
                     /locus_tag="PK513_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82646.1"
                     /translation="MTTIPGASQYIVSARLANTHGLPAQSSTLFGTGGSALSLLDAGR
                     SLNANNGIGLSARSRQLTNQLLQSTASEANALFSYAVGGSSTVEAAQTQILALRAKTP
                     TSQLARSLVQVVDDGGVAGGETGQNVDTEA"
BASE COUNT         1142 a         1058 c         1574 g         1449 t
ORIGIN      
        1 tatgagccgg tgtgggcgat cggcacggga aaaacggcga gcgtggacga tgttgtagaa
       61 atgcatggtc ttattcgtga gcagcttcaa gagcttgtgc cgaatgccga taaggtgcgg
      121 attttgtatg gtggctcgat gaagccggag aatgcgaaag agcttctatc cacagaaaat
      181 gtcgatggcg ggctgatcgg cggcgcctct ttaagcgctg aatcgtttct ggctattgca
      241 agggcggcat aaatcaatta gaaagcaatc ccatggaaag cgtacttctc gttatacatt
      301 tgtttttggc tttggccatt atcgggcttg tccttttgca gcgctctgaa gggggcggtc
      361 tggggattgg cggcggcagc ggcggtcttg gtggtcttgc aactccgcaa ggaacagcca
      421 gcttgctgac gcgcgcgacg gcgatttgcg cggcggcgtt tttttgcacc agccttattc
      481 ttggcgtttt ggctgggcgc agcacttcgc agcaggtcag tctgatggat gctttgagtg
      541 aaacgccggc ttcgattgag gcgccgcttg atgaagtaga acaaaaacct gaagaagcgc
      601 cgagcgtacc aatagctgat tagccatgac acgatatatt tttattactg gtggcgttgt
      661 ttcctctctc ggaaaaggcc tcggctccgc tgcgcttggt gcgcttttgc aggcgcgggg
      721 atataaggta cgtttatgta agcttgatcc ttatctcaat gttgatccgg ggacgatgag
      781 tccgtttcag catggcgagg tgtttgtgac cgacgacggg gcagagacgg atctggatct
      841 ggggcattat gagcgtttta ccggcgttcc ggcgcgcaag aatgacaata ttaccacggg
      901 gcaggtttat accgaagtgc tggggaaaga acggcgcggt gattatctgg gtgcgacgat
      961 tcaggttatt ccgcatatta ccaatgaaat caaagacttc attttagaga aagaaggtac
     1021 ggcggatttt gtgctgtgtg agattggcgg aacggtgggc gatattgaat ctttgccgtt
     1081 tttggaggct attcgccagt ttggtaatga ggtcgggcat gatcgcgcgc tttatgtgca
     1141 tgtgacattg cttccttata ttccagcggc aggagagttg aaaacaaagc cgactcaaca
     1201 ttctgtaaaa gagcttatga gctatggcat tcgcccggat attttgctgt gccgggcgga
     1261 tcgtgagatt catcaagaag agcgccgcaa gattgcactg ttttgtaatc ttgctgagaa
     1321 aaatgtgatt ccggcgctgg acgtttcttc gatctatgaa gtgccgctgt cttaccatgc
     1381 cgaggggctg gaccatcagg tgatggaatg tttcggcatc aaagacatcg ctaatctcga
     1441 tctttcgatt tgggagcaga ttgtccgacg gattaaagag ccggaaagta cggtgaaaat
     1501 cgcggttgtc gggaaataca ccaatttggc ggatagttat aaatcactca atgaagcgct
     1561 cattcatggc ggcgttgcca ataaggttaa ggttaaactg aaatttattg agggtgagga
     1621 ttttgaaggc ggtgtaaatc ttgcggagca tctgcaaggt gtgcacggta ttttggtgcc
     1681 gggcggtttc ggcaagcgtg gcacggaggg gaaaatcaag gctgtggaat atgcgcggaa
     1741 gcataatatt ccgtattttg ggatttgtct gggtatgcag atggcgatta tcgaggcagc
     1801 gcgcaattta tgcgggattg aggatgcgac gtcgagcgag ttgtctgacg gcgggacgca
     1861 tgtgatcggc ctgatgaccg aatgggaaac cgaagagggc ggtaaggagc agcgcagcga
     1921 ggataccgat ttgggcggga cgatgcgtct gggggcctat ccggccaagc tggttgaagg
     1981 ttcacgcgta gccgagattt acggcagtga agagattagc gagcgtcatc gtcaccgtta
     2041 tgaggtgaat atcaattatc gggagcagct agaggatcag ggtctggtgt tttccggttt
     2101 atcaccggat ggggaattgc ctgagattgt cgagcggacc gaccatccgt ggtttgtcgg
     2161 ggtgcagttt catcctgaac tgaaatccaa gccgtttgat ccgcatccac tgtttgtcag
     2221 ctttatcgaa gcggcgctca agcaaagccg tttggtttag gatgaaggcg gttcttgata
     2281 ccaatatctt tgtgggcgct ttacagcgcc gcgacggagt aaaccgggcg gtattaaaac
     2341 aggccttttt ggacgacatc acgccgttga tgagcgaggc gctttatagc gaatatcaaa
     2401 gcctgatggg gcgcgctcat cttttcgaag acagcagttt caatgcgcag gagtgcgaag
     2461 atttttttga tgatttttgc agcatttgcc gctgggtgga tgtgtattac cgctggcggc
     2521 caaatttgca cgatgaggcg gataatcatg tgctggagct ggcgatttcc gggggcgcgg
     2581 agacgctgat tacctggaat aaaaaggact ttcgccacgg gaatctggtt ttgccggaga
     2641 tcgctattct tacgcctgtg gaatattttc ggatgcagcg ggaacaatcc gtaaaaaacc
     2701 attgacagtg atagcttata tgctatcata agatagtaaa gaaaggaggt gcgctatggc
     2761 cactttgacg attcgtatgg cggatgataa agccaaccgt ttgaaagaac tcgcaaaatc
     2821 aagggatatc agcctcaata agttgattga agaatgggcc acgatgggga tcgcggaatt
     2881 tgatgcgcag gcgtcctttt tggcgcgggc ggcgcgtggg tctcgtgaac gtgggctggc
     2941 cgcccttgaa atgcttgatg cgcgggatga cgaacctcaa tccgcgcatc aaacgctgca
     3001 cgattccgaa accggcggtt tcaaacataa agacgattga agccgcgccg gggtagggcc
     3061 tgttggttta aatgtcgagt gtgttatatg cgtccaccag ggctgcttgt tggtctggtc
     3121 ttaggcgtac cgtgatggct ggtatttttg attcgttgtt gtgtttgctt gcgtttgctt
     3181 ccaaggcttt ggtcatttct ataccttgaa ctaacagggt tttcagcttg tttacgtgaa
     3241 tgcctgtagc cgtatgtaag gcctcgctta ccgctgcaat tctatctagg tttatgtcac
     3301 cgttattggg gtaggtgtta tccactttta ctgttgtcca gaaattgtct gaaatcccct
     3361 cgccgttgag gccgttttct tgttttgagg tttttttgcc ttcttgttgc ggttttgttt
     3421 gcgctttgaa caggccaagt tcttgtagcg cgtgcttcaa tggtggaatg tgatgagatt
     3481 tcatgtctgc gccatcggta taattattaa tgtcttgtgc tgtgattgat gagtgttggt
     3541 cgcttgtcgc ctttttacag attgcttggt aatcgttaaa attttgacgt tcttttctta
     3601 ggatcttacg gatttggtgc atttcgggtg tttcgccttg gttgccatga atgcgtcgct
     3661 gattttgttc tttgcttagt ttttttggtg aaacctttgt ttgagttggg tttccggtgg
     3721 ctgctgctct ttgcgctgct ctgatgtctt tcattctggc cattgctaat ccttgccgct
     3781 tctttgatta aaaaatgcac aatagtgtta tttatatgta aaaatcaagg gttttattga
     3841 aaatgtttat ggggcttccc atgagtgagg aagtgtttta gaactaaagc catgaaaacc
     3901 gtatctgtag ataaacttca aattgccaat gacaggtcgt ttgtgctgat tgccgggccg
     3961 tgccagatgg aatcgcgtga tcatgcgttc gatatgtgcg gggcattggt tgagattacc
     4021 aagaatctcg atatcccctt catttataaa agctcgttcg ataaagccaa tcgaacgtct
     4081 ttgcatggtg tgcgtggaat ggggctggat ggtgctttgc cggtgtttgc tgatcttaag
     4141 agagaatttg gcgtgccggt gctgactgac attcacacgg cggagcaatg cgctgatgtg
     4201 gctgaggtcg ttgatgtgct gcaaattccg gcgtttttat gtcggcagac ggatttgctg
     4261 gtgacggcag cgaagaccgg agcggtgatt aacgtcaaaa aaggccaatt tctggcgccg
     4321 tgggacatga agaatgtggc agcgaaaatt tccgagagcg gcaatgatca gattttgctg
     4381 accgaccgag ggacaagctt tggctataac acgctggttt cggatatgcg gggcttgccg
     4441 attatggcgc ggaccgggta tccggtgatt attgatgcga cccatagtgt tcagcagcct
     4501 ggtgggcagg gtgcttcctc tggcggagac cgggagatgg tgccggtgat tgcgcgcgca
     4561 gcggtggctg tgggcgtagc cggagtgttt atagaaacgc atcaggaccc ggacaatgca
     4621 ccctcggatg ggccaaatat ggtgaaatta gaggatatgc gcggtttact ggaaatactc
     4681 atgaaactcg acaaaatcac caaaaacgcg tagaatataa gggtaggggt agtaagccct
     4741 tgttgatttt gaataaagca ggaggcttga gcacacgtga cgaccattcc cggagcatcg
     4801 cagtatattg tttcagcgag acttgctaat acgcatggtt tacctgcgca gtcgtctacg
     4861 cttttcggga cgggcggcag tgctctgagt ttgcttgatg ccgggcgctc cttgaatgcg
     4921 aataacggga ttggcctgtc ggcgcgttct cgccagttga ccaatcagct tttgcaatcg
     4981 acagcttcgg aagctaatgc gctgttttct tatgccgttg gcgggtcttc tacggtggag
     5041 gcggcgcaga cacagattct ggcgctgcgg gcaaagacgc caaccagtca acttgcgcgg
     5101 tctttggttc aggtggtcga tgatggcggc gtggccgggg gcgaaaccgg gcagaacgtt
     5161 gatactgaag cgtgatatct ttctcaggct gttttcttat gccggttggg ctttttcaat
     5221 ttg
//
LOCUS       DAOSWR010000138         5175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10738_length_5175_cov_5.537500, whole genome shotgun sequence.
ACCESSION   DAOSWR010000138 DAOSWR010000000
VERSION     DAOSWR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10738_length_5175_cov_5.537500"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1291
                     /locus_tag="PK513_09100"
     CDS             314..1291
                     /locus_tag="PK513_09100"
                     /inference="COORDINATES: protein motif:HMM:NF017135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HOO82647.1"
                     /translation="MMNLRDGFEVVAVRGSALDSVIRQYNQLYTQARKGFLSSERLIC
                     DFDCARAVLDCSLSLVNVTSDDQDILKQLAGNDQVHATNASTAEEILHFRAGGAGKTK
                     QTFALVKPGAEDGEDRQVLTAIYGDFVKVETAADSSIPGSSLRGNVHEILTAPVQDIE
                     APNLVTFYSVTNLGGVKGVAGMLINRGLAHLSDNVPSLKAAVTLSPAWAFSRWAEGQD
                     DVPDLRDLSEQQQMQMVYRYVCDAGVKDNARNLHLFDNGASVRWIQVRNIPELMGGKD
                     DVYGDGEIVVKVNYGYDFNSEVRQKQKQKNNNGDVVVSGELASVLDLAA"
     gene            1464..1667
                     /locus_tag="PK513_09105"
     CDS             1464..1667
                     /locus_tag="PK513_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOO82648.1"
                     /translation="MATGTVKWFNPEKGYGFIVPDNGGKDVFVHISAVEAAGLRTLDE
                     GQKVEYELQENRGKEAAGNLKAA"
     gene            1771..2565
                     /locus_tag="PK513_09110"
     CDS             1771..2565
                     /locus_tag="PK513_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HOO82649.1"
                     /translation="MSEGAFAQETGQAQQPAARKTSSVQVDVRDGYRYISGFGVPHHA
                     KDQARALGIVSTPYAFRVPVKPKNTPRVTPWAPGMIFGVALDGVPIKTAVDGYWNDNQ
                     NWLKLKRKFDAYGGFIDEGPGAYMYGAIPSGLVSKDLSHVGYAADGFPIFVSKNNKFT
                     SSYRLLEGRRSDPPNGPGGVYDGAYFRDYNYIPGAGVLDRCNGVNIKGKYYIYILTPD
                     FLRIPKCWRGIPDPTFFSASSKAGSNLIVNEDDDEETRQMKNRQRR"
     gene            2720..2905
                     /locus_tag="PK513_09115"
     CDS             2720..2905
                     /locus_tag="PK513_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82650.1"
                     /translation="MEACSTASGGVMFVPDHIEGGSRPLSYREFVQQEHSEKIIGPFG
                     RKRVDEIPASRVREKVA"
     gene            2999..4300
                     /locus_tag="PK513_09120"
     CDS             2999..4300
                     /locus_tag="PK513_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO82651.1"
                     /translation="MIRLVETSTEFELSPTLAANEMVARCRAEGRQVLHMGFGQSPFP
                     VPERLQEALRAGVHRKEYLPTGGLEELRDTVAAYYKNKIGLDTDRYDVIVAPGSKLIL
                     YALQMAIEGDLLMPVPSWVSYKPQARMLRTEVIKVPTTQDDSGFHIDPEELRRVIKSA
                     RDQGQNPSKIILNYPSNPTGLTIAEKELEAIAKVCVEEDIFIISDEIYGFVAFDGVYR
                     TIAKYAPSHTAITSGLSKHLSLGGWRVGIGFIPKAVDGLYDLMCNIASETWSCVPSPI
                     QQAVIEAYKGHGDIEAHIRACTDIHGLMNRYIAAGLRDLGVYAPEPQGAFYNYPDFEA
                     HRAGMAGAGIYTSKELADALMRGCGLASLPGTAFGAEPEVLTLRLSGCDYDGAEVLAA
                     FYAGEALDEAFIRKNAPRVCAAVEAFGAFLEGLGARYKIPA"
     gene            4388..4927
                     /gene="ppa"
                     /locus_tag="PK513_09125"
     CDS             4388..4927
                     /gene="ppa"
                     /locus_tag="PK513_09125"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOO82652.1"
                     /translation="MDISKISIGDDAPNVVNAVIENPLGGVPVKYELDKASGAMFVDR
                     FLHTAMHYPGNYGFIPHTLSGDGDPVDILAIGRTPVMPGAVMPLRPVGVLMMEDDGGQ
                     DEKIIAVPGPSLHPYHADVIEYTDLRPINREQIEHFFMHYKDLESGKWSKTLGWAGVD
                     EAHKLILEGIERAKQDKAA"
     gene            complement(4992..5129)
                     /locus_tag="PK513_09130"
                     /pseudo
     CDS             complement(4992..5129)
                     /locus_tag="PK513_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1199 a         1005 c         1499 g         1472 t
ORIGIN      
        1 gtgggcagcc atcattaaat cgctggcggc ggcgtcacag ccgggttttg tgagtatgaa
       61 caggttgagc atcgcgttgg cggtgctttc gtccataccg gctgtaaatt tttttactct
      121 cgatttttcc gggcctgtta gcatttttgt acctttgtca ttggttattg gcagagggta
      181 gcagaatttt ctttgattat gcaaacttaa aaagcttttg tgtattggcc gggatggcgc
      241 atgcttgaca attgttttta tgtgcgctaa aagatttttc ataatttttt atagtgataa
      301 tcgagaaaat ggcatgatga atttaagaga tggttttgag gttgtggctg ttcgcgggtc
      361 tgctttagat agtgttatta ggcagtataa ccaactgtat acgcaagctc ggaaaggttt
      421 tttatcatca gagcgcttga tctgtgattt cgactgtgcc cgggcggttc ttgattgcag
      481 tctttcgttg gttaatgtga cgtcggatga tcaggatatt ttgaagcaat tggctggtaa
      541 tgatcaggtt catgcgacta atgcttctac tgccgaagag attttacatt ttcgtgccgg
      601 cggggccggg aaaaccaagc aaacatttgc gctggtgaag ccgggggccg aggatggtga
      661 agaccggcag gttttgacgg ctatttatgg cgattttgtg aaggttgaaa cggctgcgga
      721 tagctctatt cccggctcgt ctttacgcgg taatgttcac gagattttga cagccccagt
      781 tcaggacatt gaggcgccga atcttgtgac tttttacagt gtgaccaatc tgggcggtgt
      841 gaagggggtt gctggtatgc tgattaaccg gggacttgcg catctttcgg acaatgttcc
      901 gtcactcaaa gcggcggtta cgctttcacc ggcctgggct tttagcaggt gggctgaagg
      961 ccaggatgat gttcctgatt tacgggattt gtctgaacag caacaaatgc agatggttta
     1021 tcgttatgta tgtgatgcgg gagttaaaga taatgcgcgc aatttgcatc tttttgacaa
     1081 tggcgcatct gtcaggtgga tacaggttag aaatattccg gagttgatgg gcggaaaaga
     1141 tgatgtctat ggtgacggag agatcgtggt aaaggtaaat tacgggtatg atttcaattc
     1201 tgaagtgcgc cagaagcaaa agcaaaagaa taacaatggt gatgtcgttg tttctgggga
     1261 gttggcgtcc gttcttgact tggcagcttg attttatgcg tactaagggg tgagccgttt
     1321 tttccttgct ctttgtggat aaacagcgtt taatgccatt atctttggat gacttcgcgc
     1381 tttgactaaa aatagctcga ataccgaata ccccaaggag ctatttttga aaacttaaaa
     1441 aaaacgcgaa ggagtatcgc aatatggcaa ccggaaccgt aaaatggttt aatcctgaga
     1501 agggctacgg ctttattgtc cctgataatg gcggcaagga tgtgtttgtt cacatttctg
     1561 ctgttgaagc tgcaggtctt agaacacttg atgaaggcca aaaggtcgag tatgaattgc
     1621 aagaaaaccg tggcaaggaa gccgctggta atctgaaggc ggcttaaatt aattaaaagg
     1681 cactttaaat tgatgccagg tgagtttatg aagcgcgccg ttttaaattc tataatcgcg
     1741 tcccttattt ttttaggggg cgctttttgt ttgtctgaag gggcttttgc tcaggagacg
     1801 ggtcaggcgc agcagcctgc agctcggaaa acatcgtccg tgcaagtgga tgtgcgcgac
     1861 ggttatcgtt atatcagcgg ttttggcgtt ccgcatcatg caaaagatca ggcgcgggcg
     1921 ttgggcattg tttcgacgcc ttatgcattt cgtgtgcctg taaagccgaa aaatacaccc
     1981 agagtgacgc cttgggcgcc gggtatgatt tttggtgttg ctcttgacgg tgtaccgatc
     2041 aagacggctg ttgatggtta ttggaatgat aatcagaact ggcttaagct caagcggaag
     2101 tttgatgcgt atggcggttt tattgatgag gggccgggcg cttacatgta tggcgctatc
     2161 ccttccgggt tggtgagcaa ggatttaagc catgtcggct atgctgctga tggttttcct
     2221 atttttgtaa gtaagaataa taaatttacc tcaagttatc gtttgcttga agggcgtcgt
     2281 tccgatccgc ccaatgggcc gggtggtgta tatgacggag cttactttcg tgattataat
     2341 tatattcccg gtgcgggggt actggatcgt tgcaatggcg tgaatattaa agggaagtat
     2401 tacatttata ttttaacacc tgatttcctt cgtatcccaa aatgctggcg tggtattcct
     2461 gatccgactt tctttagcgc ttcttctaag gctggtagca atttaatcgt gaatgaagat
     2521 gatgacgaag aaacgcgtca gatgaaaaac cgtcagcgcc gttgaggttt tttaaaaaat
     2581 cctgcttttg aaataaaatg tcttttttat gctgcagtgc acagtttttg tgcactgcac
     2641 gactataatt tgtaagatag tgcaaaaaaa tatactctga gcgtgtattt tacgaatcgc
     2701 catgaaagga gatcaggcaa tggaagcatg ttcaacagca agcgggggcg ttatgtttgt
     2761 tcccgaccat attgagggtg gcagtcgccc gctttcttac agggaatttg tgcagcagga
     2821 acattctgag aagattatcg gtcctttcgg tcgtaagagg gtggatgaaa ttcctgcttc
     2881 tcgagttcgg gaaaaagtag cttaagagcg ccgcaggctt gacatagcga gcgctgtgtg
     2941 ttgcgttgtt tttccgtggt gtcttgtgac gttataatct tttaggaagg ctatcgttgt
     3001 gattagactg gttgaaactt ccaccgagtt cgagttgtct ccgacattgg cggcgaatga
     3061 gatggtggcg agatgccgtg ccgaaggtcg gcaggtgttg catatgggct ttgggcagtc
     3121 gccgtttccg gtgccagagc gtcttcagga ggctttgagg gctggtgtgc accgtaagga
     3181 atatcttccg accggcgggc tagaagagtt gcgcgatacg gtcgccgcct actataaaaa
     3241 taaaatcgga ctggatacgg atcggtacga tgtcatcgtt gcgccgggca gcaagcttat
     3301 tctctacgcc ctgcaaatgg cgattgaggg ggacttgctg atgccggtgc caagctgggt
     3361 aagttacaag ccgcaggcgc ggatgctgcg taccgaggtc ataaaagttc cgacaacgca
     3421 agatgattca ggctttcata tcgatccgga agaattgcgg cgtgtgataa aatctgcgcg
     3481 ggatcaaggg cagaaccctt ccaagattat actgaattat ccttccaatc caacggggct
     3541 gacaattgcc gagaaagaat tggaggccat tgccaaggtg tgcgtagaag aggacatctt
     3601 tatcatctcc gatgaaatct acggttttgt cgcttttgac ggtgtgtacc gcacgattgc
     3661 aaaatatgcg ccgagccata cggcgatcac atcaggccta tccaagcatc tttcgctggg
     3721 tggttggcgg gttggtatcg ggtttatccc gaaagctgtg gacgggctgt atgatctgat
     3781 gtgcaatatt gccagcgaga catggtcgtg tgtgccgtcg ccgattcagc aggcggttat
     3841 cgaagcctat aaggggcatg gggatattga agcgcatata cgggcctgca cggatattca
     3901 cggattgatg aacaggtata tcgctgcggg tttacgtgat ctgggtgttt atgcgccaga
     3961 gccgcagggg gccttttaca actatccgga ttttgaggcg caccgcgccg ggatggccgg
     4021 agccgggatt tatacgtcga aagagcttgc ggatgcactg atgagaggtt gcgggctggc
     4081 gtctttgccg gggacggcct tcggggctga gcctgaggtg ctcactttgc gcttgtcagg
     4141 ttgtgattat gacggtgcag aggttttggc ggctttttac gccggggaag cacttgatga
     4201 agcctttatt cgcaagaatg cgccgcgtgt ttgcgcggct gtggaggcgt ttggcgcgtt
     4261 tttggaaggg ctaggcgcac gttataaaat tcccgcataa atccgctatt ttaattttga
     4321 taaaggactt gctcttcaag tggttgatgg gtaagactgc ttgcattatt gaaatgaaag
     4381 gattgctatg gatatttcca aaatctctat tggcgacgat gcgccgaatg ttgtgaacgc
     4441 tgttatcgag aacccgcttg gaggcgttcc tgttaagtat gagttggaca aggcatctgg
     4501 cgccatgttt gttgatcgct ttttgcacac ggcgatgcac tatcccggta actacgggtt
     4561 tattccgcat acattgtccg gtgatggaga tccggtcgat attcttgcga ttggccggac
     4621 accggtgatg cccggcgctg tgatgccttt gcgtccggtt ggtgtgctga tgatggaaga
     4681 tgacggcggg caggatgaga agatcatcgc tgtgccgggg ccatccttgc acccgtatca
     4741 cgcagatgta atagagtata cggatttgcg tccgattaac cgtgagcaga tcgagcattt
     4801 ttttatgcac tacaaggatc tggagagcgg caagtggtcg aagacgctcg gatgggccgg
     4861 tgttgatgag gcgcataaac tgattttgga aggtattgag cgtgccaagc aggataaggc
     4921 tgcgtaaggc gttaaaggtt agactaatac atcttttttt taaagacttg actagggtcc
     4981 ggactcatag cttaaatcca gaaacataag agggaagcaa ggcatagagc ggcgaagaag
     5041 tttctggcga gtttgtcgta tcttgttgcc acgcgccgcc agtctttgag gcgtccaaac
     5101 attcgttcca cgacattgcg gtttttatgc ggtccgtagg acagaacacc acgggtttac
     5161 ccgtggatga atgag
//
LOCUS       DAOSWR010000139         5175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10739_length_5175_cov_5.226172, whole genome shotgun sequence.
ACCESSION   DAOSWR010000139 DAOSWR010000000
VERSION     DAOSWR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_5175_cov_5.226172"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="lptD"
                     /locus_tag="PK513_09135"
     CDS             <1..1018
                     /gene="lptD"
                     /locus_tag="PK513_09135"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOO82653.1"
                     /translation="RRERGEQDVQRLSVGSGWKRRLVSDYGLLTRVDANVRGDVYHTT
                     DRVVATTASGRSRSSTDTRVFPQFHMQSSYPMARPFERFQVRVEPLVAFTFAPNLTGE
                     DSIPNEDSNDVQIDASNVFESNRFPGLDRVEDRSRVTYGLRSGLFGYEGSFGDVFLGQ
                     SYRFDEDDNPFPAGSGLERQESDYVGQISASYKDIYDLDYRFQMGSNDLNSKRHEIDA
                     NADWNRFRLNVRYLYAGELEGTDIEESREQIDASAQFYFDKEWRSRFGATQDLGKEPG
                     LRKAFVGLDYLGQCLFWSFTGERNLTRDASGDSSTEILFRVGLKNLGEFEESSLRSVH
                     ESGG"
     gene            1096..3006
                     /locus_tag="PK513_09140"
     CDS             1096..3006
                     /locus_tag="PK513_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO82654.1"
                     /translation="MNILVDPTIHMWFGLFITVAAVVSFMREKVSIEITSLAVLTALL
                     VFGQVFPLPDAAGKNQIDSTALLSGFANPSLIAVLALLVMGQGMIQTDSLRILTSMFA
                     RSGAKGGWLTIFALMVFVCVMSAFMNNTPLVIIAIPIVQVLAHYVGLSESRILIPLSY
                     IAILGGMTTLIGSSTNLLVSSAMEDLGYSPLGFFEFVVPGAMLAVVGFVYVFFLLPRF
                     LPDRANMVRDLAGSKKEFVAEIDVAEDSKLIGTQCVEGRFPGLPDLNIKLIQRGGHLI
                     LPPFEGYAIESGDILIISSTRDSLTALLAQHPAFLLSEEQAQVMQKTAAQDIVEGDEG
                     GSTGNEVEALETRVLAEVMITPASRLIDMSLEHANFQRQFGVVVLGIQRRAKVVRRRL
                     GRIRLESGDVLLVAGPHSTVNAMRESTDFIVLSGSKRDLPVLGKTPIAAGIFISAIGL
                     AAAGLISIPVAAIAGAAGMIATGCLNIRQATRAIDRKIFLLVGSMLALGVILESSGAA
                     RSIAEGLLTLPFVDTPLSVLAVLFILVAIATNVLSNNACAILFTPIAMNLAGSIDVPP
                     GLNFDISHLFAVTVIFAANCSFASPIGYQTNLLVMGPGHYRFRDFARAGIPLVLLLWG
                     AYIFIVQYYYGL"
     gene            3044..3769
                     /locus_tag="PK513_09145"
     CDS             3044..3769
                     /locus_tag="PK513_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82655.1"
                     /translation="MRLSSYNPQDRYRRRNSERMAGFMVVIGVLLLAFGLGFWLGGQN
                     AGQQEWFLRTRNEALLSERETLQDSITQSRAEAQTAQARYKQLQEAYEDMLPEGPLRD
                     LVALLKQQLDEGRSAERLEFLIRSARPPRNCSEPETKRFVVMSPAYDGPESQISLAEG
                     ALIVKGKGRSAVNASGQPEAWYDPSKEVVIDFIVDGALMKSKSGVMPLRHSVVVENRE
                     YRLNVAEGARSFAKVTYDSCDYP"
     gene            complement(3932..>5175)
                     /locus_tag="PK513_09150"
     CDS             complement(3932..>5175)
                     /locus_tag="PK513_09150"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HOO82656.1"
                     /translation="NMLPIRILGELEAGGLNVAELPWQKGALTLEGTVHEPQMILAAK
                     SAGIEGLELALDIPNMRAPSLFSGTLHTDKLANLFDYLKEQKKLPVPRDTLTFLEQLN
                     NIDIIFANQKNLIFNIKNEEQNIDIKGNITIKKEGGYTGEIQAALLPLSSLTSTPDAL
                     GTVNMQGDFTTTANKISGTLQFQLAKHSMLPYGPIPLTLSGAKIKLKDWKTLSGPLEK
                     SLPCAIRNFKPEHNCSLSLQIRKSQITPSALTIKTLGTKIDAPSDQDKNKKTLLKINE
                     IDLKDLLQAFKKSNWNAAGYLEGSAILYKENGKLIIDNLYLKNKGVGILKLKEGALFD
                     LMEMEEFEKETMKLALENFHYDLLEIKASGALPDDVHISVFGKGKNPHLMQGHSFSLD
                     FKIKPNFAPLIDTLFPATKTE"
BASE COUNT         1065 a          935 c         1537 g         1638 t
ORIGIN      
        1 gcgtcgtgaa agaggcgagc aggatgtgca gcgtttgagc gttggtagtg gttggaaacg
       61 ccgcctagtg tctgattatg gattgctaac gcgagtcgat gcgaatgtgc gtggggatgt
      121 gtatcatact accgatcgag tggttgcgac gactgcgtct ggacgcagtc gttcgtcgac
      181 cgatacgcgg gtttttccgc aatttcatat gcaaagcagt tatccgatgg cgcgtccgtt
      241 tgagcgattt caggtccggg ttgagccgct ggtggcgttt actttcgcgc ctaatctaac
      301 gggggaggac agtattccta acgaagatag taatgatgtg cagattgatg ctagtaatgt
      361 ttttgagtct aatcggtttc ccggtcttga ccgggtggaa gatcgcagcc gggttactta
      421 tggtttgcgc agcgggcttt ttggttatga ggggagtttt ggagatgtgt ttttggggca
      481 gagttaccgg tttgatgagg atgataatcc gtttccggcc gggtctgggt tagaacgtca
      541 ggagtccgat tatgtcgggc aaatttctgc aagttataag gatatttacg atctggatta
      601 ccggtttcaa atggggagca atgatttgaa ttccaaacgc catgaaattg atgcaaacgc
      661 ggattggaat cgttttcgat taaatgtgcg gtatttgtat gcaggtgagc tcgaaggcac
      721 cgatattgaa gaaagccggg agcagattga cgcttcggcg cagttttatt ttgataagga
      781 gtggcgcagc cgttttggag caactcagga tttagggaag gagccaggtc tgcgtaaggc
      841 ttttgtaggc ttggattatc tggggcaatg tttattctgg tcttttaccg gagagcggaa
      901 tttaacgcgg gatgcttccg gggatagtag cactgaaatt ttatttcgtg ttggcttgaa
      961 aaaccttggt gagtttgagg agtcttcttt acgatcggtt catgaaagtg gtggttgaaa
     1021 ccagtccctt tttgcgaata tgatggccat aattcttaaa ccatagacgt atccaaatcc
     1081 aggagttttc agcacgtgaa tatccttgtt gatcccacaa tccatatgtg gttcgggctg
     1141 tttattacag ttgcggctgt ggtttcgttt atgcgcgaaa aggtttcgat tgagattaca
     1201 agtctagccg tgctgactgc cttgttggtt tttggacagg tatttccctt gcctgatgcg
     1261 gctggaaaaa accagattga ttcgacggcg ttgctttcgg ggtttgccaa tccgtccttg
     1321 attgccgtgc ttgcgctgct cgtcatgggg caggggatga tacaaacaga ttccttacgg
     1381 attttgacgt ctatgtttgc gagatcaggc gctaaaggcg ggtggcttac gatttttgcg
     1441 ttgatggttt ttgtctgtgt gatgagcgcg tttatgaata atacaccgct agtcattatc
     1501 gcaattccaa ttgtgcaggt attggcgcat tacgtgggct tatcggaaag ccgtatttta
     1561 attccgctta gctatattgc gattttaggc gggatgacga cgctgatcgg ctcgtccact
     1621 aaccttttgg tttccagtgc gatggaggat ttgggttaca gtccgcttgg tttctttgag
     1681 tttgtcgtgc cgggggcgat gttggctgtg gtcgggtttg tttatgtatt ttttctgtta
     1741 ccacgttttt tgccggatcg tgcgaacatg gttagagatc tggccggatc aaaaaaggaa
     1801 tttgtagcgg aaattgatgt tgcagaagat tctaagttga ttggtacgca gtgtgttgag
     1861 gggcgttttc caggtttgcc ggatttgaat atcaagctta ttcagcgcgg cggccatttg
     1921 attcttccac cgtttgaggg gtatgcgatc gaaagcggtg atattttgat tatttccagt
     1981 acacgcgata gtttgacggc tttgctggcc cagcatcccg cgtttttgtt gtctgaggag
     2041 caggcgcaag ttatgcagaa aactgctgcg caggatattg ttgaaggcga tgaagggggc
     2101 agtacgggta atgaagtgga agcgctggaa acacgggttt tggcggaggt tatgattacg
     2161 ccggcttcac ggttgattga tatgtctttg gagcatgcga attttcagcg tcagtttggg
     2221 gttgttgttc tggggattca gcgtcgggca aaggttgtgc ggcggcggtt ggggcgtatt
     2281 cgtttggaat ctggcgatgt tttgttggtt gccggtccgc acagcacagt caatgctatg
     2341 cgtgagagta cggattttat tgtgctttca ggatcaaagc gtgatcttcc tgtgttgggg
     2401 aaaacgccga tagctgcggg gatttttatt tctgctattg ggcttgcggc ggctggcttg
     2461 attagtattc ctgtggcagc aattgccgga gcagcaggta tgattgccac cggatgtttg
     2521 aatattcgtc aggcgacgcg ggcgattgat aggaagattt ttctgttagt tggttctatg
     2581 cttgcgcttg gtgtgatttt ggagtcttca ggtgcggcgc gttcaattgc tgaagggttg
     2641 ttgactttgc cttttgttga tacgccgctt tctgttttag ctgtgctttt tattctggtg
     2701 gcaattgcga ctaatgtgtt gagcaataat gcgtgtgcga tcttatttac accgattgcc
     2761 atgaatctgg caggaagtat tgatgttccg ccggggctga actttgatat ttctcatctt
     2821 tttgctgtga cggtgatttt tgcggcgaat tgttcttttg cttctccgat tggttatcaa
     2881 acgaatttgc tggttatggg gccgggacat taccggttcc gggattttgc gcgtgccggt
     2941 attcctcttg tcctgctttt gtggggtgcg tatattttta tcgtgcaata ttactacgga
     3001 ctttaaagta ggcttggaat aagcttcgaa gaatataggt atcatgcgac tatcatctta
     3061 taaccctcag gatcgttatc gacgccgcaa ttcggagagg atggccggat tcatggttgt
     3121 aattggcgta ttgctgctgg ctttcgggtt ggggttttgg ctgggggggc aaaatgccgg
     3181 gcagcaggag tggtttttga ggacacgaaa cgaagctttg ttgagtgagc gtgaaacgtt
     3241 gcaagacagt attacgcagt cacgagcgga ggcgcagacg gcgcaggcac gttataagca
     3301 attgcaggaa gcttacgaag atatgttgcc ggaggggcct ttgcgggatt tggtggcgct
     3361 tctgaaacag cagcttgatg aggggcgttc tgcggagcgg ttggagtttt taattcggag
     3421 cgctcgtccg ccgcgtaatt gcagcgagcc ggaaacgaaa cgtttcgtgg tcatgagccc
     3481 ggcttatgat gggccggaga gtcagattag tttggctgaa ggggcattga tcgtgaaggg
     3541 aaaggggcga tcggcggtga atgcgagcgg gcagcctgag gcgtggtacg acccttcaaa
     3601 ggaggttgtg atcgatttta ttgttgacgg ggcattgatg aaaagcaaaa gcggtgtgat
     3661 gccgctgcgt cattctgtgg tggtagaaaa ccgtgaatat cgtctgaacg ttgcggaagg
     3721 ggcgcgttct tttgctaaag tgacttatga tagttgtgat tatccctaga atctttaatc
     3781 tctttcgggt ttaattcctc gccgtttgcg gcgtaatcgt tatcgcgagc gcagcgaagc
     3841 gatccagtgc cacaagaata aattctggat tgcttcaccg cctttggcgg ttcgcaatga
     3901 catgatacct cgcctcttgg ggcgggggtg ttcattcggt ttttgtggct ggaaacagtg
     3961 tgtcgatgag cggagcgaag tttggtttga ttttgaagtc aagtgaaaag ctgtgtcctt
     4021 gcattaggtg tgggttttta cctttgccaa atacgctgat atggacatca tcgggcagcg
     4081 cccctgacgc tttgatttcc agaaggtcgt agtggaagtt ttccagtgcc agtttcattg
     4141 tttccttttc aaattcttcc atttccatga ggtcgaacaa ggcgccttct ttgagtttta
     4201 agattccgac acctttattt ttcaggtaga gattatcgat tatgagcttg ccgttttctt
     4261 tataaaggat tgcgctgcct tcaaggtagc ctgccgcgtt ccagtttgat tttttaaatg
     4321 cttgaagcag gtctttgagg tcaatttcgt tgattttgag taaagttttt ttatttttgt
     4381 cttggtcgga tggggcatcg atttttgtgc ccaacgtttt gatggttaag gcgctagggg
     4441 tgatttggct tttacgaatt tgcagtgaca gggagcagtt gtgttctggt ttgaagttac
     4501 gaatagcgca ggggagcgat ttttccagag ggccggaaag agttttccag tctttcagtt
     4561 tgatttttgc gccgctgagc gttaggggga ttgggccata gggcaacatg ctgtgtttag
     4621 ccagttgaaa ctggagcgtg ccggatattt tattggctgt ggttgtgaag tcaccttgca
     4681 tattgacggt gccgagggcg tcaggggtgg atgttaggct cgatagtgga agcagggcag
     4741 cttgaatttc acctgtgtat cctccctctt tttttatggt tatatttcct tttatatcaa
     4801 tattttgctc ttcattttta atgttaaata ttaagttttt ttggttggcg aaaataatat
     4861 cgatattgtt gagttgttct agaaaagtca gggtatctct ggggaccggg agtttctttt
     4921 gttctttcag atagtcgaaa agatttgcca gtttatctgt atgtaaagtt cctgaaaaaa
     4981 gcgaaggcgc acgcatattg ggaatgtcga gagcgagttc aagcccttca atgccagcgg
     5041 attttgcagc gaggatcatt tgcggttcat gaactgttcc ttcaagtgtc agggcgcctt
     5101 tttgccatgg caattccgcg acattaaggc ctcctgcttc cagttcaccg agaatgcgga
     5161 ttgggagcat gtttt
//
LOCUS       DAOSWR010000140         5169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_10759_length_5169_cov_4.480250, whole genome shotgun sequence.
ACCESSION   DAOSWR010000140 DAOSWR010000000
VERSION     DAOSWR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10759_length_5169_cov_4.480250"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK513_09155"
     CDS             <1..228
                     /locus_tag="PK513_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO82657.1"
                     /translation="QLNPYAVLEGALIAARAVGLDCGPMSGFDAAGVKDEFFADSAVQ
                     PNFLCNLGYGDASALHPRAPRLTFDEACSIL"
     gene            240..935
                     /gene="tsaB"
                     /locus_tag="PK513_09160"
     CDS             240..935
                     /gene="tsaB"
                     /locus_tag="PK513_09160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOO82658.1"
                     /translation="MSDFQNILALDTALGGCVAAVVAGEREAVRCEAMVRGQAERLIP
                     FAQEVMEEAGLDYGDLDAVLCTIGPGAFTGLRIGMSAARAFGLSLDIPVIGVTTLQAL
                     AMNYVAEHGLDCSVVLETKRSDFYIQDFNAQGLAVSAPRAELAEVLLKALPRNSVLIG
                     DGVVRFINEIHGLWNDVDYGYGLADMAMVAQVLQDQGLVDGVFERDARPVYLRDADVS
                     MPKKASRILEGAG"
     gene            1024..1428
                     /locus_tag="PK513_09165"
     CDS             1024..1428
                     /locus_tag="PK513_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HOO82659.1"
                     /translation="MTITINREEYLAFTTEIVSAYLSNNTIQAGEIPSVIEQVYKTLV
                     NVNADSHVSTDRPQPAVPIKKSVMPDYIICLEDGKQLKMLKRHLKTAYGMSPEEYRER
                     WGLPMDYPMVAPNYAAQRSKLAKDIGLGTKGR"
     gene            complement(1425..2228)
                     /gene="proC"
                     /locus_tag="PK513_09170"
     CDS             complement(1425..2228)
                     /gene="proC"
                     /locus_tag="PK513_09170"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOO82660.1"
                     /translation="MSAQPNIVLIGCGKMGGALLQGWLDQNIDANFSVIEPHDISITE
                     ITHHKTVEAAAKDLKSAALIVLAVKPQTMATLCTELKNHINEDTTILSIAAGQSLTRF
                     SQYFAPTQPVIRAMPNTPAAIGKGMSIAVANKHTSQSAKALAETLLKTAGKFEWTDDE
                     SLLDAVTALSGSGPAYVFYLIEMLEKAGNKAGLPIEMATTLARQTVIGAAALAEAQSE
                     IPAKTLRKNVTSPGGTTAAALEILMDGRTQNLFNEALQAATARSRELNS"
     gene            complement(2235..2738)
                     /locus_tag="PK513_09175"
     CDS             complement(2235..2738)
                     /locus_tag="PK513_09175"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOO82661.1"
                     /translation="MTTQLETYEDDIHPLDSVEDVLSANNWTFERMAEDELAVDIMGR
                     SGSYKLLFIWQGEMHALQFCAQFDLQIAGNNIKIAREVLLELNESLWMGHFDLPAKNT
                     RPAFRHTCLLRGSSPALITELIDDMVDIALAQCERHYPLFTLLASANDINDAALSLAM
                     METAGES"
     gene            complement(2762..2998)
                     /locus_tag="PK513_09180"
     CDS             complement(2762..2998)
                     /locus_tag="PK513_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HOO82662.1"
                     /translation="MIKSKDKMLDDIAHVADGTLSIFSGLGENIREDVKTRIDEIAHR
                     LDLVPREDFDRLEALVQTQEKRIQTLEKSLKTKK"
     gene            3134..3946
                     /gene="lgt"
                     /locus_tag="PK513_09185"
     CDS             3134..3946
                     /gene="lgt"
                     /locus_tag="PK513_09185"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO82663.1"
                     /translation="MWEFPNIDPVAISLGPIDIRWYALAYLAGFLLGWWYCKRLADLD
                     ENRRPDKLDIDDFLPWAIAGVILGGRFGYVVFYNLEYYTAHPAEILMLWEGGMSFHGG
                     ALGVIVALLIYPSIHKFSHLRLADLVCACVPLGLFFGRVANFINGELFGRVTNSPLGM
                     VFPRGGPYPRHPSQLYEAVLEGLVLFLILFVLIRSDWVRARPGIVSGMFLAGYGCARA
                     FVELFREPDSHIGFVFGGAVTMGQVLSTPMIVVGLGLVIYAVARKRSDAQSA"
     gene            3930..4940
                     /locus_tag="PK513_09190"
     CDS             3930..4940
                     /locus_tag="PK513_09190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO82664.1"
                     /translation="MPSPLEEIVRQKIRADGPMLIGAFMALALGHPEHGYYMKGDPFG
                     RAGDFVTAPEVSQLFGEMIGVWAADLWMQMGAPERLAMVECGPGRGTLMADILRASRN
                     VAGFHDALRIHLVEMSPVLRGKQREALAEYEVVWHEDFKGVPGDVPMIVIGNEFLDAL
                     PFEQYVGDVQRKVGLDEDAQLVFVPMEGDVYEFAPYRNDFVKCVCSRLKAQGGAALFI
                     DYGHVHSGPGDTFQAIKGHEYVDVLSHIGDADLTSHVNFEALQRGVDIGVAGPVEQGD
                     FLRALGIGLRARMLAEKADVQQVDMIEKGLHRLIDSDQMGSLFKVIGFYYHDKPLAPA
                     GF"
     gene            4909..>5169
                     /locus_tag="PK513_09195"
     CDS             4909..>5169
                     /locus_tag="PK513_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOO82665.1"
                     /translation="MTNLLLQQDSDPLYFKHSDFVGLQNNTVFHGFFSRRGGVSRDIF
                     AALNCGPGSGEALSIVQKNRQIVSEIVGCEAKNLLSLHQIHSN"
BASE COUNT         1019 a          957 c         1668 g         1525 t
ORIGIN      
        1 caactcaacc cgtacgccgt cctcgaaggc gcgctgatcg ccgcccgggc ggtgggtctg
       61 gattgcgggc cgatgtcggg ctttgatgcg gcgggcgtga aggacgaatt ctttgcggat
      121 agcgctgtgc agccgaattt cctgtgtaat ctggggtatg gggatgcatc tgcgctgcat
      181 ccacgggctc cgcgattaac atttgatgag gcgtgctcaa tactttaagt aaagatgcta
      241 tgagtgactt tcaaaatata ctggcgctgg atacggcgct ggggggatgt gtggctgcgg
      301 tggttgccgg tgaacgcgag gctgtgcgct gtgaggcgat ggtgcgcggg caggctgagc
      361 gtctgatccc atttgcgcag gaagttatgg aagaggcagg gctggattat ggggatcttg
      421 atgctgtgtt gtgcacgatc gggccgggag cgtttacggg cttgcggatt gggatgtcgg
      481 cggcgcgggc ttttgggctg agtttggata ttcctgttat cggggttaca actttgcaag
      541 cgttggctat gaattatgtt gctgagcatg gtttggattg cagtgtagtt ttagaaacga
      601 agcgtagcga tttttatatt caggatttta atgcgcaagg gttggctgta tctgcgccgc
      661 gggcggaatt ggctgaggtt ttgttgaagg ctttgccaag aaatagtgtg ttgattggtg
      721 atggcgtcgt acgatttatt aatgaaattc atgggttatg gaatgatgtt gattatggat
      781 atggattggc ggatatggcg atggtggcgc aggttttaca ggaccaaggg cttgtagacg
      841 gcgtttttga gcgtgatgca cggccagtgt atttacggga tgcggacgta tcgatgccca
      901 aaaaagcgtc tcgtatttta gaaggtgcgg ggtgagtatt gttcttcggc gcattttctt
      961 gacaaatatt ttaccttacc tagattgtac tttgtgttcc atttctgaag agagggcgtt
     1021 ttcatgacaa taacaataaa ccgggaagaa tatcttgctt ttacaacgga aattgtttcg
     1081 gcttacttgt ctaataatac aattcaggcc ggggaaattc cgtcggtgat tgagcaggtt
     1141 tataaaacgc tggtcaatgt gaatgcggat agccatgtta gcacggatcg tcctcagccg
     1201 gcagttccga ttaaaaagtc tgtgatgccg gattatatta tttgtcttga ggatggtaag
     1261 cagctcaaaa tgcttaagcg gcatttgaaa acggcttatg ggatgagtcc ggaggaatat
     1321 cgtgagcgct gggggctgcc gatggattac cccatggtgg cgccgaatta tgcggcgcaa
     1381 cgctcgaagc tggctaaaga tattgggttg ggtactaaag gtcgttaaga atttaattcg
     1441 cggctgcggg ctgtagcggc ttgcagagct tcgttgaaga ggttctgggt gcggccatcc
     1501 atgaggattt caagcgcggc ggcggtggtg ccgccggggc tggtgacgtt tttacgtaag
     1561 gttttggcgg gtatttcgct ttgtgcttcg gccagcgcgg cggcgccgat gacggtctgg
     1621 cgggcgaggg tggtggccat ttctataggg aggccggctt tgtttccagc tttttccagc
     1681 atttcgatta aataaaagac gtaggctggg ccgctgcctg agagggcggt gacagcgtcg
     1741 agcagggatt catcgtctgt ccactcaaat tttccggcgg ttttgagcag ggtttcggcc
     1801 agtgctttgg cactttggct ggtgtgttta ttggctacag cgatgctcat gcccttgccg
     1861 atggcggcag gggtgttggg catggcgcgg attaccggct gggtgggggc gaaatattga
     1921 gaaaatctgg ttagagattg tccggcagcg atagacagga tggtggtgtc ttcgttgata
     1981 tggtttttca gctctgtgca gagtgtggcc atagtttgtg gtttgacagc tagcacgatg
     2041 agggcggcgg attttaagtc tttggcggcg gcttcaacgg ttttgtggtg cgttatttcg
     2101 gtgattgaga tgtcgtgcgg ttcaatgacg gagaagtttg cgtcgatatt ttggtcaagc
     2161 cagccttgta ataaggcgcc gcccatttta ccgcagccga tcagaacaat gttgggctga
     2221 gcgctcatag ggtcttatga ttccccggct gtttccatca tcgccagtga cagcgctgca
     2281 tcgttgatgt cattggcgct ggcgagcagg gtgaagagcg ggtagtggcg ttcgcattgg
     2341 gccagagcga tatcgaccat gtcatcgatt aattcggtta taagtgcggg agatgagccg
     2401 cgcagcaagc aagtgtggcg gaatgccggg cgtgtgtttt ttgcaggcag gtcgaaatgg
     2461 cccatccaaa gggattcgtt tagttccagc agcacttcgc gcgcaatttt gatgttgtta
     2521 cccgcgattt gtaaatcgaa ttgggcgcaa aattgcagag cgtgcatttc cccttgccag
     2581 ataaagagca acttatagct tccgctgcga cccatgatat cgacagccag ttcatcctcg
     2641 gccatgcgtt cgaatgtcca gttgttggca ctgagcacat cttcaacact gtcgagcggg
     2701 tggatgtcgt cttcgtaggt ttcaagctgc gtggtcataa agtctttccg tgattgttgt
     2761 tttatttctt tgtctttaga gatttttcta aagtctgaat gcgtttttcc tgagtctgta
     2821 cgagtgcttc gaggcggtca aaatcttcgc gtggcacaag gtcaaggcgg tgggcaattt
     2881 catcaatgcg tgtttttacg tcttcacgaa tattttcacc taaaccactg aaaatactta
     2941 atgttccgtc tgcaacatgg gcgatgtcat ccagcatttt atctttgctt tttatcattg
     3001 ttccagctct tgtgtgtagc tttgagtcag tatcatggct ctgtgtagag tccaaattgt
     3061 tttgagattt ttaatcttta ggcctgtgga aatttttatt tctattttgg ggtgatgatt
     3121 tataaagggg gccatgtggg aatttccaaa tatagatcct gtggcgatca gtttagggcc
     3181 gattgatatt cgttggtatg cgctggcata tttggccgga tttttgcttg gctggtggta
     3241 ttgcaaacgg ctggctgatc tggatgagaa ccggcgtccg gacaagttgg atattgatga
     3301 ctttttgccc tgggcgattg ccggggtgat tttgggtggg cgcttcggat atgtggtgtt
     3361 ttataatctt gagtattaca cggcgcatcc ggcagaaatt ttgatgttat gggagggggg
     3421 gatgtccttt catggcgggg cgctaggggt aatcgttgcg ctcttgattt atccgtccat
     3481 tcataaattt tcgcatctgc ggctggctga ccttgtgtgt gcgtgtgtgc ctttggggtt
     3541 gtttttcggg cgggtcgcca attttatcaa tggtgaattg tttgggcggg tgacgaattc
     3601 gccgttaggg atggtgtttc cgcgcggtgg gccatatccg cggcatccaa gccagcttta
     3661 tgaggcggtg ctggaagggt tggtgttgtt tttgatcctg tttgtgttga tccggagtga
     3721 ctgggtgcgg gcgcggcccg ggattgtatc gggtatgttt ttggccggct atggctgcgc
     3781 gcgggcgttt gttgagctgt tccgcgagcc ggattctcat attggatttg tatttggcgg
     3841 ggcggtgacg atgggacaag tgttgagtac gccaatgatt gtggtagggc tggggctggt
     3901 tatttatgcg gtagcgcgga aacgatccga tgcccagtcc gcttgaagag atcgtaagac
     3961 agaaaattcg tgcggatggg ccgatgttga tcggcgcgtt tatggccctt gcgctggggc
     4021 atcctgagca tggttattat atgaagggcg acccgtttgg gcgggctgga gattttgtaa
     4081 ctgcgcctga ggtttcgcag ctttttggtg aaatgatcgg cgtatgggcg gcggatttgt
     4141 ggatgcagat gggcgcgcct gagcggctgg ccatggtgga atgcgggccg gggcgtggga
     4201 cgttgatggc tgatatattg cgtgctagtc gaaatgtagc agggtttcat gatgctttgc
     4261 ggattcatct ggttgagatg agtccggtgt tgcgcgggaa acagcgagag gcgttggccg
     4321 aatatgaggt ggtttggcat gaagatttta aaggtgtgcc gggtgatgtg ccgatgattg
     4381 tgattggcaa tgagtttttg gatgctttgc cgtttgagca atatgtgggt gatgtgcaac
     4441 gcaaggtcgg gctggatgaa gacgctcagt tggtttttgt gccaatggag ggtgatgtat
     4501 atgaattcgc tccgtatcga aatgattttg tgaagtgcgt ctgtagtcgc ttaaaggcgc
     4561 agggcggggc agcactgttt attgattacg ggcatgtgca ctcagggccg ggcgatacat
     4621 ttcaggcgat taagggccat gaatatgttg atgtgctgag tcatattggt gatgcggatt
     4681 tgaccagcca cgtgaatttt gaggctttac agagaggtgt tgatattggt gttgccgggc
     4741 cggtggagca gggggatttt ctgcgggcgc tcggtattgg tttacgggcg cggatgttgg
     4801 cagaaaaagc cgatgtgcag caggtcgata tgatcgaaaa aggtttgcat cggttgattg
     4861 attcagatca aatgggatcg ttgtttaaag tgattggatt ttattatcat gacaaacctc
     4921 ttgctccagc aggattctga tccgctttat tttaagcata gtgattttgt ggggctgcaa
     4981 aataatacgg ttttccatgg tttttttagc cgtcgcggtg gtgtaagccg ggacattttt
     5041 gccgcgctaa attgtggacc cgggtcgggg gaggctttaa gtattgttca gaaaaaccgc
     5101 cagattgtga gcgaaattgt cgggtgtgag gctaaaaact tgttgagttt acatcagatc
     5161 catagcaac
//
LOCUS       DAOSWR010000141         5071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11053_length_5071_cov_5.148923, whole genome shotgun sequence.
ACCESSION   DAOSWR010000141 DAOSWR010000000
VERSION     DAOSWR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11053_length_5071_cov_5.148923"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK513_09200"
     CDS             <1..217
                     /locus_tag="PK513_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82666.1"
                     /translation="AALLAQPEIMSAVIYGDAQAHLSAEIVPASLHADITGAVQRANA
                     ELPDYARIKDFQIKTKENDHELLQQAG"
     gene            192..>518
                     /locus_tag="PK513_09205"
     CDS             192..>518
                     /locus_tag="PK513_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HOO82667.1"
                     /translation="MSFYNRLVKETEDQRQELYSVPQLMDGIQGNISKETYIAYLTEA
                     YHHVSHTVPFLMTMGSKIPQSKKWMHKAIIEYQGEEVGHEEWILNDIESAGGDKEAAH
                     NSAPNLE"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <529..870
                     /locus_tag="PK513_09210"
     CDS             <529..870
                     /locus_tag="PK513_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HOO82668.1"
                     /translation="QVLISYNYDYMTRKNPVGFFGMVFMLESTSTQIANKGADAVKDK
                     LGLPKKAFSYLYSHGELDISHMKFFEETVNKITDPNDQAAIIEVAQNTFRLFANVLRA
                     IPHEGQVKHAA"
     gene            860..1636
                     /locus_tag="PK513_09215"
     CDS             860..1636
                     /locus_tag="PK513_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO82669.1"
                     /translation="MPLKGKTVYITGGTGGIGTPLVQYLENAGAEVTAHDIEKEGDLV
                     ANMDKICAYLKDNTPDILINMAGYNVLDYCENQNLEAIVDLNMMVPMRLSQAVLPGMK
                     ARNSGQIINMGSMTALIPLPHLTGYVAAKAGLKGFNDALRRELGGTNIKLTHIAPRAV
                     KTPMNSGVKAEVNKRTKVKYDDPSDVARWIFEAIIEQKPDVRFGWPERFFAFMHANFP
                     FIIDNGLQKNRQIGEDALNTHHQTKEIKDEKTTTLHRAAS"
     gene            1599..2348
                     /locus_tag="PK513_09220"
     CDS             1599..2348
                     /locus_tag="PK513_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82670.1"
                     /translation="MKKLPLYTALLLSITLPVHAEDVWTIRTPQTQTAPQTVETSVGQ
                     MAVSPMDQDIAMLQKEWAHIKYQMSNEDQQLEAIHRLEAQAASVSSKYPGNAEPKIWE
                     GIILSTDAGIVKGMSALGKVKKAKVLFETSLKQNPTALDGSAHTSLGSLYYQVPGWPV
                     AFGDDEEAEKHLKHALQLNPNGIDTNFFYGDFLLQDDRLEEAKAYLNRALQAPDRPNR
                     ELADAGRRQEIKAALAQIDQKMKDKSKPRYN"
     gene            2361..3017
                     /locus_tag="PK513_09225"
     CDS             2361..3017
                     /locus_tag="PK513_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82671.1"
                     /translation="MRVLLVEDDMMLGKATAEGLKTSFAVDWCESAEDASAALKATPY
                     DLVILDINLPGQSGLDLLKNLRRANDDRPVLFLTARDTVPQRIEGLNAGADDYLVKPF
                     DLDELIARSSALIRRSQGRANPEIVCGDISYDSTAKQAKQNGKTVKLSGRELAVLEIL
                     MTHLSKVISKTQIEEHIYDWDSGDIESNTIEVHISALRRKLGKDLIKTIRGVGYMIEK
                     "
     gene            3014..4354
                     /locus_tag="PK513_09230"
     CDS             3014..4354
                     /locus_tag="PK513_09230"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO82672.1"
                     /translation="MNKNYSLRRRLILWISLSILCATLLALLASYIFASHEVEEVYDA
                     QLVHYAKVLLQLTQHEIMEDEGFDLGIENPDLQHKYERKFSFRIWVDNEIITQSPNTK
                     AFAGFEAPPGFSNQNIDDHEWRFFVFIVPENKIKIEVSESYDIRYELIVQFIAALILP
                     ALMFLPVIFLIIWIGVRKILKPVVKISAMVDKRGSSDLSPIHTNKAPQEIIPLLQALN
                     RLFTRIEKSFVREREFTDNAAHELRTPLAAMKTQTQVLLKKAQAMPDCREGLDNLHAS
                     IDRASKMVDQLLAFSRLQANQIEFETVDLSSLAQDILKDISPLAVKKRIEMRADIAPV
                     ITIKGNRNALEIMLRNLIDNAIKFTPADGRIDVSVFEEGHKAVLKIFDTGPGIKDTEK
                     DKVFERFYRVQKNKKGSGLGLSMAKWVCDVHGAEIYLSDNDPNGSIVTFSMKRT"
     gene            complement(4619..>5071)
                     /locus_tag="PK513_09235"
     CDS             complement(4619..>5071)
                     /locus_tag="PK513_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82673.1"
                     /translation="SYTGAAVGGVTGADSKCQALADAEGLGGTYKAWISDSNGLTAPS
                     ASFTQASIPYRLVNGRRIADDWADLINAANPTTITIDETGALQVNKKVWTNVHTNGNQ
                     INLSGNCSDWGSTAGSAYNGESWRLDSYWSYSNATACSTALHLYCFEQ"
BASE COUNT         1374 a         1157 c         1273 g         1257 t
ORIGIN      
        1 agccgcgctg ctggcccaac ctgaaattat gagcgctgtc atctacggtg atgcgcaggc
       61 ccatttaagc gcggaaatcg ttccggcctc tttgcacgct gatatcacag gggccgtgca
      121 gcgggctaat gcggaattgc cggattacgc ccgtatcaag gattttcaaa ttaaaacaaa
      181 ggagaatgac catgagcttt tacaacaggc tggttaagga aacggaagac caaaggcaag
      241 aactctacag tgttccgcaa ctgatggacg ggatacaggg taatatctcg aaagagacgt
      301 atattgctta cctgacggaa gcctatcatc atgttagtca caccgtacca tttctgatga
      361 ccatgggttc aaaaattccg cagtccaaga aatggatgca caaagccatt atcgagtatc
      421 agggagagga agtcggccat gaggaatgga ttctgaatga cattgaatcc gctggcggtg
      481 ataaagaagc cgcccacaac tccgcaccca atctggagnn nnnnnnnnca ggtgctgatc
      541 tcttacaact acgactacat gactcgcaaa aacccggttg gatttttcgg catggtgttc
      601 atgctggaaa gcacctcaac acagattgcc aataagggtg cagatgcggt taaagacaag
      661 ctgggactgc cgaagaaggc gttttcctac ctctattcac atggtgagtt ggacatctct
      721 cacatgaaat tctttgagga aacggttaat aaaatcaccg atccgaacga ccaggctgcc
      781 attattgagg tggcgcagaa tacattccgg ctgtttgcca atgtcctgcg cgctattccg
      841 catgaagggc aggtgaaaca tgccgcttaa aggaaagacc gtttacatca ccggaggaac
      901 gggtggaatt ggcacaccgc ttgtacagta tctggagaac gccggtgctg aagtgaccgc
      961 ccatgacata gagaaagagg gtgatcttgt cgctaacatg gataaaatct gtgcttatct
     1021 gaaggataac acgccggata ttcttatcaa catggctgga tataatgtgc ttgactactg
     1081 cgaaaatcag aatttagaag ctattgtcga tctgaacatg atggtaccga tgcggctgag
     1141 ccaggctgtg ctacctggta tgaaggctcg caactccggc cagattatca acatgggttc
     1201 gatgaccgcg cttatcccgc tgccgcatct gacaggttac gttgccgcca aagcgggatt
     1261 gaaaggtttt aacgatgcct tgcgccggga actgggcggt acgaatatca aactcaccca
     1321 cattgcgccg cgagccgtta aaacaccgat gaatagcggt gtaaaggcgg aagtgaataa
     1381 acgcaccaag gttaaatatg atgatccttc cgatgtggcg cgatggattt ttgaggccat
     1441 tatagagcaa aaacctgatg tgcgctttgg ttggccggaa cgtttctttg cctttatgca
     1501 tgccaacttc ccttttatca ttgataacgg tctgcagaaa aaccgtcaga ttggcgaaga
     1561 cgctttaaac actcaccacc agacaaagga gattaaggat gaaaaaacta ccactttaca
     1621 ccgcgctgct tcttagtatc actctgcccg ttcatgcgga agatgtttgg acgatacgaa
     1681 ctccgcaaac ccagacagcg ccgcagacgg ttgaaacctc tgtcggacaa atggccgtct
     1741 cgccgatgga ccaggatatc gcgatgctgc aaaaagaatg ggcacacatc aaataccaga
     1801 tgagcaacga ggaccagcaa cttgaagcca ttcacaggct agaggcgcag gccgcgtccg
     1861 tgagctccaa atatccgggc aatgctgaac cgaaaatctg ggaagggatt attctttcca
     1921 ccgatgccgg tatcgtcaaa ggtatgtctg cactgggcaa agttaaaaaa gccaaagtgc
     1981 tttttgaaac ttccctgaaa caaaatccga cggctttgga tggttcagcg catacttcgc
     2041 tcggttcgct ctattatcag gtgccaggct ggccagtggc tttcggggat gacgaggaag
     2101 ctgaaaagca tttaaaacat gccctgcaac tcaacccgaa tggtattgat acgaactttt
     2161 tttatggaga ttttctgctg caggatgacc gtctggaaga ggctaaggcc tatctaaacc
     2221 gggcactgca ggctcctgat cgtccgaacc gcgaactggc cgatgccggg cgcagacagg
     2281 aaatcaaggc tgctcttgcg caaattgatc aaaagatgaa agataagagc aagcctcgat
     2341 acaattaaag gattggctgt ttgcgcgtac ttctggtcga agatgatatg atgttgggaa
     2401 aagccacggc agagggactg aaaacttctt ttgccgtgga ctggtgtgag agcgcggaag
     2461 atgcctcagc ggctcttaaa gccacgcctt acgatctggt gatccttgat atcaacctgc
     2521 cggggcagtc cggccttgat ctgctcaaaa atttacgcag agccaatgac gaccggcctg
     2581 tgctttttct gaccgcgcgg gatacggtac cgcagcgcat agagggctta aatgctggag
     2641 cggacgatta tctggttaag ccctttgatc tggatgaact gattgcccgg tcaagcgctc
     2701 tgatccgccg ctcgcaaggc cgggctaatc ctgaaatcgt atgcggtgat atcagctatg
     2761 actctacagc caaacaggcc aagcagaacg gcaagacagt taagctgtcc gggcgcgaac
     2821 tagctgtgct ggaaattctg atgacccatc tgagcaaggt gatcagcaag acgcagatcg
     2881 aagaacatat ctatgattgg gacagtggcg acattgaaag caacacgatc gaggttcata
     2941 tttccgcgct gcgccgcaag ctcggcaagg atttgatcaa gacaatacgc ggtgtgggtt
     3001 atatgattga aaaatgaata aaaactattc tctccgccgc cgtctcattt tatggattag
     3061 tttgtccatc ctttgtgcaa cgctgcttgc cttgctggcc agttatatct ttgcaagcca
     3121 tgaagtcgaa gaagtttatg acgcgcagct tgtccattac gccaaagtgc ttttacagct
     3181 tacccagcac gaaatcatgg aggatgaggg ttttgatctg ggcattgaaa acccggattt
     3241 acagcataag tacgagcgta aattcagttt tcgtatctgg gtcgataatg agattattac
     3301 gcagtccccc aacacaaaag cctttgcagg ctttgaagcg ccgccgggat tttccaacca
     3361 gaatattgat gatcacgaat ggcgtttttt tgtttttatt gttccggaaa acaagatcaa
     3421 gattgaagtt tctgagagtt atgatattcg ctacgagttg attgttcagt ttatagcagc
     3481 gcttattctg cctgcgctta tgtttcttcc tgtgattttt ttgattatct ggattggcgt
     3541 gcgcaagatc ttaaaaccgg ttgtaaaaat ctcggctatg gtcgataagc gtggcagcag
     3601 tgatctctct cctatacata caaataaagc accgcaagaa attataccac tcctgcaggc
     3661 tttaaaccgt ttatttaccc gtattgaaaa aagctttgtc agagaacgtg aatttacaga
     3721 caacgccgcg catgagttgc gcacgccgct ggcagcgatg aaaacacaaa cgcaagtttt
     3781 gcttaaaaag gcgcaggcca tgccggattg cagggaagga ttggacaatc ttcatgcatc
     3841 cattgatcgt gcaagcaaaa tggttgacca gcttttggct ttctcacgat tacaagccaa
     3901 tcaaatcgaa tttgagacgg tcgatctttc ttctttagct caagatattt taaaggacat
     3961 atccccgttg gctgtgaaaa aaagaattga gatgagggcc gacattgctc cggtgattac
     4021 aatcaaaggc aacaggaatg cgcttgaaat catgctgcgt aatttgattg acaatgccat
     4081 taaattcacc ccagcggatg ggcggattga tgtttctgtt tttgaagagg gtcataaagc
     4141 tgttttaaaa attttcgata ccggccccgg catcaaagat actgaaaagg acaaggtttt
     4201 cgagcgtttc tatcgcgtcc agaaaaacaa gaaaggcagc ggccttggcc tgtccatggc
     4261 caaatgggtg tgtgatgtgc acggcgcaga gatatatcta agcgataatg accccaacgg
     4321 ttcgatcgtt acatttagca tgaagcgcac ctgatgcggt ggattttaat tatagcctct
     4381 attttgggag cctcttcctg tggccataaa atgtagctta actttgccac aaagctgtcc
     4441 aacaaagtag gaccacctct atacaacatt tggaagatgc ctgtgctgac gtcatacaga
     4501 cgggggattg ataatgtggt gaatgtgatg gtgataattt aaggcccggc gcgccgcgca
     4561 gtccatggtt ttgtatctct ttgtttggga tagtaagaat ataccggaaa gagaaaggtt
     4621 attgttcaaa acagtagagg tgtaaagctg ttgagcaagc tgtcgcattg ctatatgacc
     4681 agtaagagtc aagccgccat gattccccat tgtacgcaga tcctgctgtt gagccccagt
     4741 cgctgcaatt accgctaaga ttaatctggt ttccattagt gtgcacgttt gtccaaactt
     4801 tcttgttcac ctggagggct ccagtttcat ctatggtaat tgtggttgga ttcgctgcgt
     4861 ttatcaggtc agcccaatca tcggcaatgc ggcgcccatt aacaagccga taggggatcg
     4921 aggcttgagt aaagctggcg gatggtgcgg taagcccatt tgaatcacta atccaagctt
     4981 tataggttcc gcccaaacct tccgcatctg ccaaggcctg gcatttgctg tctgctcccg
     5041 ttaccccgcc aacggcagct cccgtataag a
//
LOCUS       DAOSWR010000142         5023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11210_length_5023_cov_6.032609, whole genome shotgun sequence.
ACCESSION   DAOSWR010000142 DAOSWR010000000
VERSION     DAOSWR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11210_length_5023_cov_6.032609"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK513_09240"
     CDS             <1..299
                     /locus_tag="PK513_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO82674.1"
                     /translation="TPQDLALVDARKAHEMFQMVGVPILGIIENMSTHICSNCGHEEY
                     IFGHGGAREEAEKLGVAFLGEIPLSANIRSKADAGEIFDLGDDIVKMFVAIQSG"
     gene            425..2404
                     /locus_tag="PK513_09245"
     CDS             425..2404
                     /locus_tag="PK513_09245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO82675.1"
                     /translation="MLKISEYYDPHPSFYAVAAALWLVGFGFALMLLEAYEAESFLLA
                     FLCMGACVICVLAAAPLSALRAVISAPIVWLALGFWALAALSAAFSDIPFVSFIYFCF
                     FSVFPLSAFVVACGGSCALLKPLGYGAAVVFGALALSCLTQYFFMPEMLFHGTVNWPL
                     ANPNSLAGLLSLGFFAAVGWMLAAPGRGQANIGLAFAVLLFAALLTTGSRGAFAAMIP
                     AVALFLWGLRGYLDQHKRCLGALVVLSVLMFVAMSVLTPLGVKSPASFIETTLSGAQS
                     VLWSRPAIWAAALEVFRAHIWSGTGVGTFFLYYPEVRSAGDPSTAGLMVHSDPLQFAV
                     EMGVMAPLLFYGFILAAVWMSVRALKKLPLDDARLVCILAPFCALMALIIHAHITFHF
                     QVLSILMVAGVLLGFWFLQVRAVLYASIQETEEPYCNPCVRWGLAVPVVGILSIFSQF
                     QVSEILVNRAQNDLMVGAGESYANKINRAGRLSYNQNARALVAATHLPLGIVQLNAPL
                     MPRDALQTLEGQLCSLLDRAELANPRLAQIYFARAEMAAYIQPFLPEKGEGERYDVAE
                     ELRKALAIDPLHLGSRMKLADLSLRVGRMEEALDILEPGLNWWYKNQRPRLFLEKTYG
                     LARQLGRDEIADKAALEFVRYFPEEKLPGMEREYE"
     gene            2397..3044
                     /locus_tag="PK513_09250"
     CDS             2397..3044
                     /locus_tag="PK513_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82676.1"
                     /translation="MSEEFASPALLSILCVLMGAAVFFAPHVWLLERLRNSAVQSVSL
                     GRAVMGAAMAALFTAALFSQIYALIDGHGVLWPEVFVLAALAIGSFILGRLRGMGLSR
                     LFVKVPLAWFVINLKVFGFMLATAVIIGLFTAVNIPVRESVFVTGAVWIAALYKLHVS
                     VQRQNSGKEKEAFHRLLWPLVLGLLFWMLPLLLNELIHSEGFPEMLHRSPPLQRA"
     gene            3185..3712
                     /locus_tag="PK513_09255"
     CDS             3185..3712
                     /locus_tag="PK513_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HOO82677.1"
                     /translation="MNDEDDILRYDKMVEAALRGVVKKSIEEVMEQHGTTGGMPGEHH
                     FYITFLTDFPGVKIPDYLRERYPGEMTIVLQYQFSDLNVDKEKMNVTLSFNNVPERLE
                     IPLAAISIFADPSVNFALQFQPLGESLEDADLAILDELDDDPDDDGSGGGKGKSGKGG
                     GDTGQVISLDQFRKK"
     gene            3726..4634
                     /locus_tag="PK513_09260"
     CDS             3726..4634
                     /locus_tag="PK513_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82678.1"
                     /translation="MYSPDPGPYKVLLKRGEFVDAARLNDDGSARVVPFKLYYPVEHD
                     QGGLPTILWSHGFGGNRDGASFISRYLASHGYNLLHITHRGTDSSLWEGKPGHPWDIL
                     RKAQVSRETTLNRFYDVPFVLDQLPVWAADNPDVGAHMDLSRFGMSGHSFGAMTSQVM
                     AGQMFPDASGKLISLREPRFRAGILYSPVPIAHLTDAAETDIYGPIDIPLLHMTGTQD
                     SSPLHDFGYERRLAVHDYSGAAEKYLQVLEGGDHMVYAGSRGKLGANPLRDEHEAQIK
                     AAALAFWDAQLKDDEAARAWVKENLS"
     gene            4655..>5023
                     /locus_tag="PK513_09265"
     CDS             4655..>5023
                     /locus_tag="PK513_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO82679.1"
                     /translation="MSVGMDEYAVEIADLKKIYAGNKKSPEKEALKGVSLNIPRGSIF
                     GLLGPNGAGKSTIINIMAGLVMKSSGMVNIWGIDIDKDLRNAKAAIGIVPQELAFDAF
                     FTPAQALDLMAGLYGVPKDQR"
BASE COUNT          968 a          937 c         1574 g         1544 t
ORIGIN      
        1 caacgccgca ggatttagcg ctggttgatg cgcgtaaggc ccatgaaatg tttcagatgg
       61 tcggtgtgcc gatcttgggc attatcgaga atatgagtac gcatatttgc tcgaattgcg
      121 ggcatgagga atatattttc ggtcatggag gggcgcggga agaagcggaa aaactcggcg
      181 tggccttcct gggtgaaatt cccttaagcg ccaatattcg aagcaaagcc gatgcgggag
      241 agatttttga tttgggtgat gacattgtga agatgtttgt ggcaatccag agcggctgac
      301 ttaagaatgg atggggctat tctatccaag ccgaagaggt atgatggtat catgtacgag
      361 agaagaatct cgtaaatctt ttcttgctgc tatggattgc ttcgcttacg cttgcaataa
      421 taccatgttg aaaatatctg aatattacga tcctcatcca tcattttatg ctgttgctgc
      481 ggcgctttgg cttgtcggct ttgggtttgc gctgatgctg ttggaggctt atgaggcaga
      541 gagttttctg ctggcttttt tatgcatggg ggcttgcgtg atttgcgtat tagctgctgc
      601 gccgttatca gcactcagag ccgtaattag tgcgccgatt gtgtggttgg cactggggtt
      661 ttgggcgctg gcggcgttga gcgcggcttt ttcggacatt ccttttgtca gttttattta
      721 tttttgtttt tttagcgtgt ttcctttgtc tgcgtttgtg gtggcttgtg gcggttcatg
      781 cgcgttgttg aagcctttgg gttatggagc ggcggtggtg tttggcgcac tggcactttc
      841 gtgcctgacg caatattttt ttatgccgga gatgcttttt catggcaccg tgaactggcc
      901 gctggccaat cctaattcac tggcagggct tttgtctctt gggttttttg cggctgtcgg
      961 gtggatgttg gcggcgccgg ggcgcgggca ggcgaatatt gggttggcgt ttgcggtttt
     1021 gttgtttgcg gcgttgctta caacggggag ccgcggggcg tttgcggcaa tgattccggc
     1081 tgtggctctg tttttgtggg gtctgcgtgg gtatcttgat cagcataaaa ggtgtttggg
     1141 cgctttagtg gttttaagtg ttttgatgtt tgttgcgatg agcgtgctta cgcctttggg
     1201 ggtgaaatcg ccggcgagtt ttattgagac gacattgagc ggtgcgcaat cggttttatg
     1261 gagccgtccg gcgatttggg cggcggcgct ggaagttttt cgtgcgcata tttggagcgg
     1321 gacgggggtt ggcacgtttt ttctgtatta tccggaagtg cgcagcgctg gagacccttc
     1381 gaccgcgggg ttaatggtac atagcgatcc gttacagttt gctgttgaga tgggtgttat
     1441 ggctccgctt ttattttacg gttttattct ggcagcggtt tggatgagtg tacgcgcttt
     1501 gaaaaagctg cctttggatg atgcgcgtct ggtttgtatt cttgcgccgt tttgcgcttt
     1561 gatggctttg attattcatg cgcatatcac atttcatttc caagttttat cgattttgat
     1621 ggttgcgggg gtgttgctcg gtttttggtt tttacaggtg agggctgttt tgtatgcatc
     1681 gatacaagag actgaagaac catattgtaa tccttgcgtt cgttggggtt tggctgttcc
     1741 tgttgttgga attttatcca tttttagcca atttcaggtg agtgaaattt tggtcaatcg
     1801 ggcacaaaat gatttgatgg taggggcagg cgaatcttat gcgaataaga ttaatcgggc
     1861 cgggcggctg tcatataatc agaatgcacg agcgcttgta gctgcgacgc atttaccgct
     1921 ggggattgtt cagttgaatg cgccgttaat gccgagagat gctttacaaa cactggaagg
     1981 ccagctttgt tcattgcttg accgggcgga gcttgcaaac ccgcgtttgg cgcaaattta
     2041 ttttgcacgg gcggaaatgg cggcctatat ccagccgttt ttgcctgaga agggggaggg
     2101 tgaacgttat gacgtggcgg aggagttgcg aaaagcgctg gcgattgatc cgctgcattt
     2161 ggggtcacgg atgaagctgg cggatttgtc tttacgtgtt gggcgcatgg aagaggcttt
     2221 ggatattttg gagccggggc ttaactggtg gtataaaaat cagcgcccac gcctgttttt
     2281 agaaaagacg tacgggttgg cgcggcagtt ggggcgtgat gagattgcag acaaagcggc
     2341 tttggaattt gtgcggtatt tccctgaaga aaagttacct ggtatggagc gggaatatga
     2401 gtgaggaatt tgccagtcca gcgcttttaa gtattttgtg tgtgctcatg ggggccgcgg
     2461 tgttttttgc gccgcatgtc tggttattgg agcgtttgcg caattcggcc gttcagagtg
     2521 tttcattggg gcgggcggtg atgggggcgg ccatggctgc gctttttaca gctgcgcttt
     2581 tttctcaaat ttatgcgtta attgatgggc atggcgtact ttggccagaa gtttttgtgt
     2641 tagcagcttt ggctataggg agttttatct tgggtcgcct tcggggcatg gggctttcgc
     2701 gcctgtttgt taaagtgccc ctggcgtggt ttgttattaa tctgaaggtc tttggtttta
     2761 tgctcgcgac agcggttatt attggtttat ttacagctgt taatattcct gtgcgtgaat
     2821 ctgtttttgt tacaggcgcc gtctggattg ccgcgcttta caagttgcat gtgtctgtgc
     2881 agcggcagaa ttcggggaaa gagaaggagg ctttccacag gttgctttgg ccattggtgc
     2941 tggggttgct tttctggatg ttgccgctgc ttttgaatga gcttatccat tctgaaggtt
     3001 ttcctgagat gcttcatcgc tcaccgccgt tacagcgcgc ttaaaagcta tcccgatttt
     3061 gtccacaggc aagagtgggt tatttgtatc tacgggctgg tcttttatgg gtgtttcatg
     3121 cgattcaaga ggaagggtat ttctactctt gatttaatgt tccttatttg cactgaatat
     3181 atccatgaat gacgaagacg acatattgcg ctatgacaag atggttgaag ctgccttacg
     3241 cggggtggtc aaaaagtcta ttgaagaggt tatggagcaa catggaacaa ccggtggcat
     3301 gcccggtgag caccatttct atattacgtt tttgactgat tttccggggg tgaagattcc
     3361 cgattatttg cgggagcgtt atcccggtga gatgacgatt gttttgcaat atcaattttc
     3421 tgatttgaat gttgataaag aaaaaatgaa tgtgacgctg tcgtttaaca atgtgccaga
     3481 gcggcttgaa attcctttgg cggcgatcag tatttttgct gatccgtcgg tgaattttgc
     3541 gttgcagttt cagccattgg gcgaatcgct ggaggatgcc gatctggcta ttcttgatga
     3601 gttggatgat gatccggatg atgatggctc cggcggtggt aaaggcaagt ccggtaaggg
     3661 cggtggtgat accggtcagg tgatttcact ggaccagttc cggaagaaat aggctgggtg
     3721 cccttgtgta ttctcctgat cctggcccgt ataaagttct tttgaagcgt ggggaatttg
     3781 ttgatgcggc gcgtctaaat gatgacgggt cagcgcgcgt tgttccgttt aagctgtatt
     3841 atccggtgga gcatgatcag ggcgggttgc cgacgatttt gtggagtcat ggatttggcg
     3901 gcaaccgcga cggggcgtcg tttatttcgc ggtatctggc ttctcatggt tataatctgt
     3961 tgcatattac gcatcgcggg acggattcga gcctgtggga aggcaagccc gggcatccgt
     4021 gggatatttt gcgcaaggcg caagtgagcc gcgagacgac gctcaaccgg ttttatgacg
     4081 tgccgtttgt gctggatcaa ttgcccgtat gggcggcgga taatccggat gttggcgcgc
     4141 atatggattt atcgcggttt gggatgtcgg ggcattcatt tggcgcgatg accagccaag
     4201 tgatggccgg gcagatgttt cctgacgctt cgggaaagct tatttctttg cgtgagccgc
     4261 gattcagggc cgggattttg tattcaccgg tgccgattgc gcatcttaca gatgcggcag
     4321 aaacagacat ttacgggccg attgatattc ctttgcttca tatgacaggg acgcaggatt
     4381 cttcgccgct gcatgatttt ggctatgagc ggcgtttggc ggtgcatgat tattcgggcg
     4441 cggctgaaaa atatctgcag gtgctcgaag gtggcgatca tatggtctat gcgggttcgc
     4501 gtggtaagtt gggggctaat cctttgcggg atgaacatga ggcgcagatt aaggcggcgg
     4561 cgctggcgtt ttgggatgcg cagcttaagg atgacgaagc ggcgcgggcg tgggttaaag
     4621 aaaatctatc ctagactttg tgtggtgatg gtttatgtct gtaggcatgg atgaatatgc
     4681 cgttgaaatc gccgatctaa agaaaattta tgcgggtaat aagaagagcc cggagaagga
     4741 ggctttgaag ggagtcagcc ttaatattcc gcgtggttca attttcgggc ttttggggcc
     4801 taacggggca ggaaaatcca caattatcaa tattatggcg ggccttgtga tgaaatcatc
     4861 gggcatggtg aatatctggg gcattgatat tgataaggat ctgcgcaatg ccaaggcagc
     4921 gattgggatt gtgccacagg aactggcctt tgatgcgttt ttcacgccgg cgcaagcgct
     4981 ggatttgatg gcgggcttat atggcgtgcc gaaggatcag cgc
//
LOCUS       DAOSWR010000143         5013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11240_length_5013_cov_4.853772, whole genome shotgun sequence.
ACCESSION   DAOSWR010000143 DAOSWR010000000
VERSION     DAOSWR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11240_length_5013_cov_4.853772"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..943)
                     /locus_tag="PK513_09270"
     CDS             complement(77..943)
                     /locus_tag="PK513_09270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO82680.1"
                     /translation="MKSAIKKLLPQSVINAARALRDGHAVNTLAPLNFTAENLKPANS
                     LEFSSIWADKDIATCWQKDCDEISSVFPESDKWGGINPGDRRALYYLISALKPQNVLE
                     VGTHIGASSVYIAKAMSRYAQEEASLTTVDILDVNDPQTGAWAGLGMERSPQGNLEAL
                     DCGELCNFVVNSAQDYMAALADDDGKRFDFIFLDGDHTAKGVYHEMALALKILKPGGV
                     ILLHDYYPDGQALFRDGNIIYGPFKALESVRGQAPNVTVKPLGTLAWPTKQGSSNTSL
                     ALVMQSAGEDGE"
     gene            complement(950..1849)
                     /gene="pdxS"
                     /locus_tag="PK513_09275"
     CDS             complement(950..1849)
                     /gene="pdxS"
                     /locus_tag="PK513_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOO82681.1"
                     /translation="MGKTSGSNTVINIKTGLAEMLKGGVIMDVVTAEQAKVAEDAGAV
                     AVMALERVPSDIRKAGGVARMSPPEVIEQVMAAVSIPVMAKCRIGHFAEAQILEAMGV
                     DFIDESEVLTPADEEYHINKHDFKVPFVCGAKDLGEACRRIGEGAALMRTKGEPGTGN
                     IVEAVRHMRKINAQISRVTNMDESELMSYARDLGAPYEILKMVRAEGKLPVPNFAAGG
                     VATPADAALCMQLGAETVFVGSGIFKSDNPEKFARAIVKATAHFDDPAVVLEASKELE
                     GVAMRGTEISEIEEPMKMANRGW"
     gene            complement(1950..3005)
                     /locus_tag="PK513_09280"
     CDS             complement(1950..3005)
                     /locus_tag="PK513_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HOO82682.1"
                     /translation="MSAIIPKIDGPFLKGMGMVVMLAACAGALTALPVLKTLHIHGLI
                     IAMVLGMVYANSARKYIPESWISGIDYAARRVLRLAIVLYGFRLTFQDIAAIGVSGVV
                     ISALMVGLTFLIGYVVGVKLLKIDREVTILTAAGAAICGAAAVLATEAVIKAKPYKSM
                     VAVATVVIFGTLAMFLYPAAYRLELVPMNLDEMGVYIGGSVHEVAHVVAASAAVDPAT
                     SDAAVIVKMVRVMMLAPFLILLGLWFWRGAENIAQKNEEGQKPALIPWFAIYFIAVAG
                     FNSLHLLPAQMVSSLISFDTFLLTMAMGALGMETSITKFKGVGLKPVYLALLLFIWLI
                     FGGFFITKIVMGALGGI"
     gene            3106..3765
                     /locus_tag="PK513_09285"
     CDS             3106..3765
                     /locus_tag="PK513_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82683.1"
                     /translation="MTDNKSIGIMPGSYNPVHPGHVDTAQKVRDQHRLSEIWLLPITQ
                     STTKRSLQAEFNQKVQMCKIIAMPHSQWLKVSTAYRDHGSGLIGQLQSYKRTIEDISE
                     KNLDTDLYIVGGEDFAKRLLWAARMLHIAAAMSNVVSAGKKINWGVVINASKRLEKAS
                     DILRTPILTTGRSTLTSSSEIRQQFAAGSTPGVLPEELATYIAEHKLYKDKDPDYSLV
                     A"
     gene            complement(3775..3951)
                     /locus_tag="PK513_09290"
     CDS             complement(3775..3951)
                     /locus_tag="PK513_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82684.1"
                     /translation="MRNHPLFVLSNDLSNSAMSTQDKDLEKEDIKPLTGVHIAPKGHG
                     AGCACCMPEEENDQ"
     gene            3950..4195
                     /locus_tag="PK513_09295"
     CDS             3950..4195
                     /locus_tag="PK513_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82685.1"
                     /translation="MSAPLVETLSLEQAKEAIYTSLTNDNEDIDLHIANLKAAMNAAG
                     EKEAVFAPDRLSQNNRAGRKMMQSYFKKRGVKVVFAS"
     gene            4185..4940
                     /locus_tag="PK513_09300"
     CDS             4185..4940
                     /locus_tag="PK513_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO82686.1"
                     /translation="MRASSFIYAPSQEALNILHQDGDVLVLSKPAGLLSVPGKAPEHA
                     DCLETRAKEQFPGSLLVHRLDMDTSGIFVMAMNKAAQANLGKQFERRKVQKTYIARVW
                     GVPEEREGCVDLPLRCNWENRPLQMVCHEHGRLSQTDWRIIEHSASPLLRAQRSNPEC
                     PGMDGRVGSDDPSRNDEIFKISRVELKPLTGRSHQLRVHMAALGHPILGDRFYAHERA
                     LNAADRLQLHAESITLHHPKDGTLTTFTDTCPF"
BASE COUNT         1319 a         1417 c         1108 g         1169 t
ORIGIN      
        1 gtttcctttc tctagtgcaa gtttgaagca atatgaacaa aattaaagat tatgtcaata
       61 atatttgtgc caacctctat tcgccatctt caccggcgct ctgcatgacc aacgccaaag
      121 aggtattgct tgagccttgc ttcgtcggcc acgccagcgt tcccagcggc ttgaccgtaa
      181 cattcggcgc ttgaccccgc acactttcca gcgccttaaa cggcccgtat ataatattcc
      241 cgtcccggaa caaggcttgc ccgtccggat aataatcatg cagcaaaatc accccgccgg
      301 gctttaaaat tttcagcgcc agcgccattt catgataaac gcccttggcc gtatgatcgc
      361 catcaaggaa aataaaatca aaacgttttc cgtcgtcatc agctagcgcc gccatatagt
      421 cttgcgccga attgacaaca aaattgcaca attccccgca gtccagcgct tccaggtttc
      481 cctgcggaga acgctccatc cctaggccgg cccaagcgcc tgtttgcggg tcgttaacgt
      541 ccagaatatc cacggttgtc aggcttgctt cttcttgcgc atagcgagac atcgctttgg
      601 cgatatagac gctggaagcc ccgatatgcg tgccaacctc cagcacattt tgcggtttta
      661 acgcgctgat cagataataa agcgcacgcc gatcaccggg attaatgccg ccccatttgt
      721 cactttcagg aaatacactg gaaatttcgt cgcagtcttt ttgccagcat gtggcaatat
      781 ctttgtctgc ccaaatggat gaaaactcaa ggctattggc aggttttaaa ttttctgccg
      841 taaaattaag cggagccagc gtgttaacag catgcccatc gcgtaaggca cgcgctgcgt
      901 ttataacaga ctgaggcaaa agctttttga tggcgctttt catagcgctt taccaccctc
      961 tgttggccat tttcatcggc tcttcaatct cggaaatttc cgtgccgcgc attgccacac
     1021 cttccagctc tttcgatgct tccagtacaa ccgccggatc atcgaaatgc gcggtcgctt
     1081 tcacgatggc acgcgcgaat ttttcagggt tatcggattt gaaaatcccg gaaccgacaa
     1141 acacggtttc tgcgccaagt tgcatacaca gcgctgcatc tgctggtgta gctacgccgc
     1201 ctgccgcaaa gttcggcacc ggtaatttac cttcggcccg caccattttc aaaatttcat
     1261 acggcgcgcc cagatcccgc gcatagctca tcagctcaga ttcatccatg ttggtcacgc
     1321 gggaaatctg cgcgttaatt ttgcgcatgt ggcgcacagc ttcaacaata ttgccagtgc
     1381 ccggctcacc cttggtgcgc atcagcgccg caccctcgcc aatccggcgg catgcttctc
     1441 ccagatcctt cgcgccacag acaaacggca ctttaaaatc atgcttgttg atatgatatt
     1501 cctcatccgc aggcgttaga acttcgctct cgtcgataaa gtcaacgccc atggcctcaa
     1561 gaatttgagc ttcggcaaaa tgcccgatcc ggcacttggc cattacgggg atcgacaccg
     1621 ccgccatcac ctgctcgata acttccggcg gactcatccg cgcaacgcca ccagccttgc
     1681 gaatatccga aggaacgcgt tccaaagcca taacagccac cgcgccagca tcctcggcca
     1741 ccttggcttg ctctgccgtt acaacgtcca taatgacgcc gcctttcagc atctcggcca
     1801 ggccggtctt tatattgatt acggtgttag agccgctcgt tttgcccatg actatacaat
     1861 ccttatattg tgtgcgctcc ggcacttaaa tctacgaaag agcgctattt atacatatct
     1921 atggcgtaaa tgcaaagagt ctatcgtgtc taaataccac ccagcgcgcc catgacgatt
     1981 ttggtaataa aaaatccgcc aaaaatcagc cagatgaata ggagcaaggc cagatagacg
     2041 ggcttaaggc ctacgccttt gaatttcgta atgctggttt ccatgcccag cgcacccatc
     2101 gccatggtca gcaaaaacgt atcaaagctg atgaggctgc tgaccatctg cgcaggcaac
     2161 aaatgcaagg agttaaatcc ggccaccgca ataaaatata ttgcgaacca tggaatcaga
     2221 gcaggctttt gcccttcctc atttttttgt gcaatattct cggccccgcg ccagaaccat
     2281 aaacccagca agatcaggaa tggcgccagc atcattaccc gcaccatttt tacaatcacc
     2341 gccgcatctg aagtggctgg atccaccgct gcgctggccg cgaccacatg ggcgacttcg
     2401 tggaccgatc caccaatata cacgcccatt tcatccagat tcatgggcac tagctcaaga
     2461 cggtatgcgg cgggatacaa aaacatagcc agagttccaa agatgacaac cgttgccacg
     2521 gcgaccatgc tcttataggg cttggctttg ataacagcct cggtcgccag caccgccgcc
     2581 gcgccacaaa tcgccgcccc cgcagccgtg agaatagtaa cctccctatc aatcttgagc
     2641 agctttaccc caaccacata accaatcagg aaggtcagcc ccaccatcag tgcgctgatc
     2701 acaaccccgg aaacgcctat ggctgcaatg tcctgaaagg tcaggcgaaa cccgtagagc
     2761 acaattgcca gccgcaaaac ccgccgcgcc gcataatcaa tcccgctgat ccagctctcc
     2821 ggtatgtatt tgcgagcgga attcgcgtaa accatgccca agaccatcgc gatgatcagg
     2881 ccgtggatat gcagcgtctt aagaacaggc agggcggtca aagcacccgc gcaggctgcc
     2941 agcataacca ccatccccat gccctttaaa aaaggtccat caattttggg aattatcgcg
     3001 ctcataaaca aacctataaa attatgaaaa tattgacatt ttgtcttttt ggttttacaa
     3061 gctcgatact acaataaaat accgtttaaa acctgagtta aggccatgac tgataataag
     3121 tcgataggca tcatgccggg atcctataat ccagttcatc ctggtcacgt tgatacagca
     3181 cagaaagtca gagatcaaca cagactttct gagatttggt tgttgccaat cacacaatct
     3241 acgacaaaac ggagcttaca ggctgaattt aatcaaaaag ttcaaatgtg caagattata
     3301 gccatgccgc attctcagtg gcttaaagtt tcaacggcgt atcgtgatca cggaagcggc
     3361 ctgattggac agttacaatc atacaaaaga acaattgaag acatatcaga aaaaaatctg
     3421 gacaccgatt tatatattgt gggtggagaa gactttgcca aaagattgtt atgggccgcg
     3481 cgtatgctcc acattgctgc tgcaatgagc aatgttgtga gtgcgggtaa aaaaataaac
     3541 tggggagtgg tcattaatgc tagcaaacgt ttggaaaaag catcagatat tttaagaaca
     3601 cccatactaa caacaggccg aagcacactg acatcttcaa gtgaaatcag gcagcaattt
     3661 gccgcaggaa gtacgcctgg ggttcttcca gaagaattgg caacctacat tgccgagcat
     3721 aagctctaca aagataaaga ccccgattac tcattagtag catagacttt gagtttattg
     3781 gtcattctct tcctccggca tgcagcacgc gcatcccgct ccatgcccct tcggcgcaat
     3841 atgcactccg gttagcggct taatatcttc tttttcgaga tctttatctt gcgtgctcat
     3901 agcgctgttt gataaatcat ttgaaagaac aaataaagga tgattacgca tgagcgcccc
     3961 ccttgtcgaa accctttcac tcgaacaggc caaagaagcc atttacacct cgctgaccaa
     4021 cgacaatgag gacatagatc tgcatatagc caatctcaag gctgccatga acgctgctgg
     4081 tgaaaaggaa gcggtctttg cgcctgatcg tttaagtcag aataaccgcg caggccggaa
     4141 aatgatgcaa agctatttca aaaagcgcgg cgtgaaagtt gtctttgcga gctagctctt
     4201 tcatttacgc gccctcgcaa gaggcgctca atattctcca tcaagatgga gacgtgctgg
     4261 tgctctctaa acccgccggg ctgctcagcg ttccgggcaa agcgccggaa catgccgatt
     4321 gccttgaaac acgcgccaaa gaacaattcc ccggaagcct gctggtccac cgcctcgata
     4381 tggatacttc cggcatattc gttatggcga tgaataaggc tgctcaggcc aatctcggca
     4441 aacagttcga acgccgaaaa gtgcaaaaaa cctatatcgc ccgcgtttgg ggcgtgccgg
     4501 aggagcgcga aggctgcgtc gatcttcccc tgcgctgcaa ttgggaaaac cgcccgctac
     4561 agatggtctg tcacgaacat gggcgccttt ctcaaaccga ctggcggata atcgagcata
     4621 gcgcctctcc gttattacga gcgcagcgaa gcaatccaga atgtcccggt atggatggcc
     4681 gcgtcgggtc tgatgaccct tctcgcaatg acgaaatatt caaaatatcc cgcgttgaac
     4741 tcaagcccct caccggccgt tctcaccaac tccgcgtgca tatggcggcg ctgggccatc
     4801 ccattctggg tgatcgcttt tatgctcatg aacgcgcact caatgccgca gaccgcctcc
     4861 aactccatgc tgaaagcatc acgcttcacc accccaaaga cggcacgctc accaccttca
     4921 ccgatacttg tccattttaa taaccaaata gcatgacttt acaattctgc cgatcacatt
     4981 aatgtttggt actgaacaga taaacaggag aac
//
LOCUS       DAOSWR010000144         5012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11244_length_5012_cov_4.851321, whole genome shotgun sequence.
ACCESSION   DAOSWR010000144 DAOSWR010000000
VERSION     DAOSWR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11244_length_5012_cov_4.851321"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PK513_09305"
     CDS             complement(<1..549)
                     /locus_tag="PK513_09305"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOO82687.1"
                     /translation="MSLRSIIKSTGGYLPEKILTNQDLEKMVDTTDEWITQRSGIKSR
                     HIAADDQTTADLATFAGRAALEASGLEPGEIDGVIVATTTPDQTFPAVAVKVQAALEI
                     PAGLAFDVQAVCSGFIYALATADNFIKCGQARRILVIGAEKLSSIVDWNDRTTCVLFA
                     DGAGAIILEGQESADDISARGIH"
     gene            complement(568..876)
                     /locus_tag="PK513_09310"
     CDS             complement(568..876)
                     /locus_tag="PK513_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005789 - endoplasmic reticulum membrane
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PigN domain-containing protein"
                     /protein_id="HOO82688.1"
                     /translation="MSEDKNTKKQSNKKKRNIYYALVGCFAGMVVAFFLKDTPFAYLV
                     AVLFPVFFIYDVLGGVLGTKSLIGKERKKEWSFARIELIVTGSFYIGFMLLFTILKML
                     "
     gene            complement(888..1922)
                     /gene="plsX"
                     /locus_tag="PK513_09315"
     CDS             complement(888..1922)
                     /gene="plsX"
                     /locus_tag="PK513_09315"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOO82689.1"
                     /translation="MSRSRIIALDAMGGDYGPESVIPGAVLTLKAHPQLGFILFGDEG
                     QIGPLLDRESALKAVSTVVHTEHKILSNEKPSAVLRKSKGSSMRLAIEAVKEGRATAV
                     VSAGNTGALMALAKMVLTPLPGIHRPAIASIFPTRQSDTIMLDLGANVLVDAENLVQF
                     AVLGAVFAKLHKNVAQPSVGLLNVGSEEMKGPDHVREAAAILSEVEFPGHYYGFVEGD
                     DITKGTVDVVVSDGYAGNVALKTAEGVGSLTTHLFKKNVMRDPLGILGSMVAYFALKR
                     FQHQVDPRRYNGGVFLGLNGLCVKSHGGCDALAFSSAVKLAAELAEQGYVEMVGREIE
                     CLSEQNLLIE"
     gene            complement(2025..2204)
                     /gene="rpmF"
                     /locus_tag="PK513_09320"
     CDS             complement(2025..2204)
                     /gene="rpmF"
                     /locus_tag="PK513_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018996927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOO82690.1"
                     /translation="MAVPKRKTTKSKRNMRRAHDALSTENWVEDANTGEPKRRHHIDL
                     KTGTYKGKQVLEARD"
     gene            complement(2293..2904)
                     /locus_tag="PK513_09325"
     CDS             complement(2293..2904)
                     /locus_tag="PK513_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOO82691.1"
                     /translation="MSKAPKRILETEWSHLFDVEAMNKAEETLTIAPDALACGRLSQR
                     LGVESVDALEADIRVSHHAGEMSYYIKGQIRADVTQACVVTLEPVHSHIEDDFEAWYA
                     DPEVAVSITKARHEKLNEKGHGELPILDEKDDPEALIEGKIDLGELVTQYLSLSINPY
                     PHAEGVEFEELERKMGALEEDDLVKNPFAALKDWKDKISGSES"
     gene            complement(2910..3398)
                     /locus_tag="PK513_09330"
     CDS             complement(2910..3398)
                     /locus_tag="PK513_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HOO82692.1"
                     /translation="MTRDVYANVLVCVRQPKFYEKYGVPDTFDGRFDLMVLFIFLVFQ
                     RMLEEGRDEALHFNQALFDVCFEDMDQTLREMGIGDMGVPKHMRKMMKAFNGRMHAYE
                     QALTDGDLAAALRKNLYGSAVEADEKKIAKMEVKALEILAFLKIQDMDAILSGKIAFE
                     KE"
     gene            3519..3974
                     /gene="bamE"
                     /locus_tag="PK513_09335"
     CDS             3519..3974
                     /gene="bamE"
                     /locus_tag="PK513_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOO82693.1"
                     /translation="MILQISKKTLLLSAAILTLGTSACTPMVAQRGNMVEDFQMQQIT
                     PHESTRSDVLRNLGSPTSKSTFDPNVWYYIGQKTEKRGILDPQVVEERIILVAFNEEG
                     FVEAIQDIDRERMNIPYAREKTPTHGNETTVMQQFLGNLGKFNPQTGEQ"
     gene            complement(4037..>5012)
                     /locus_tag="PK513_09340"
     CDS             complement(4037..>5012)
                     /locus_tag="PK513_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOO82694.1"
                     /translation="IRGLAISMEATAWPVLVICGGIYVAYDLAGLYGIAISATSMLSL
                     AGMVVALDAYGPVTDNAGGIAEMADLPEGVRNTTDALDAVGNTTKAVTKGYAIGSAGL
                     AALVLFAGYTEEIAHYLPELADIRFDLTDPYIVIGLFLGGLLPYLFSALSMTAVGRAA
                     ASVVIEVRRQFKEIPGIMEGKAKPEYGTAVDLLTKAAIGEMVVPSLLPVLAPAVLWVV
                     IYKVTGDMAAAFQCLGAMLLGTIVTGIFVAISMTAGGGAWDNAKKYIEDGNYGGKGSD
                     AHKAAVTGDTVGDPYKDTAGPAVNPLIKIANIVAILLVAIVAKFATGS"
BASE COUNT         1402 a         1568 c          896 g         1146 t
ORIGIN      
        1 gtgaatgccg cgcgcggaaa tatcgtccgc actctcctgc ccttcaagaa taatcgcccc
       61 ggccccgtca gcaaacaaaa cgcacgtcgt ccggtcattc caatcaacaa tagaagaaag
      121 cttctccgcg ccaatcacca gaatgcgccg cgcctgcccg catttaataa aattatccgc
      181 cgttgccaac gcatagataa agccgctaca aaccgcctgc acatcaaaag ccagcccggc
      241 tggaatctct aaagccgcct gcaccttcac cgccaccgcc ggaaatgtct gatccggcgt
      301 cgttgtcgcc acaatcacac catcaatttc gcccggctca agcccggaag cttccaaagc
      361 cgcacgcccg gcgaaggtag ccaaatccgc cgtcgtctga tcgtccgctg caatatgccg
      421 ggatttaata ccgctgcgct gcgtgatcca ctcgtcggtc gtatccacca tcttctccaa
      481 atcctgattg gtcaggattt tctccggaag atagccgcct gttgatttta taatggagcg
      541 taacgacatt gttctttttc tcagaggtta taacattttc aaaattgtaa acagcagcat
      601 aaatcctata tagaaagaac cagtcacaat aagctctatt ctagcaaagg accattcttt
      661 cttacgctct ttgccaatca aagacttagt tccaaggacc ccacccaaaa catcatatat
      721 aaaaaagacc ggaaacaaga cagccaccaa ataggcaaag ggtgtgtctt ttaaaaagaa
      781 tgcgacaacc ataccagcaa aacagccaac cagagcataa taaatattac gcttcttttt
      841 attactttgc tttttggtat ttttatcttc actcacaaaa aacgtcccta ttcaataagc
      901 aaattctgtt ctgacaaaca ctcaatctcg cgcccgacca tctcgacata accctgctcc
      961 gccaattccg cagccagctt caccgcagaa gaaaacgcca gcgcgtcaca ccccccgtgg
     1021 cttttgacac acaaaccgtt caagcccaaa aacaccccgc cgttatagcg gcgcggatct
     1081 acctgatgct gaaaacgttt cagcgcaaaa tacgccacca tgctccccaa aatccctaaa
     1141 ggatcgcgca tcacattctt tttaaacaaa tgcgtagtca aactccccac gccttccgcc
     1201 gttttcagag caacattccc cgcataccca tcactgacca caacatcaac cgtgcctttg
     1261 gtaatatcat cgccttcaac aaaaccatag taatgccccg gaaactcaac ctcgctcaga
     1321 atcgccgcag cctcccgcac atgatcaggc cccttcattt cttccgagcc cacattaagc
     1381 agccccacac tcggctgagc cacattcttg tgcaatttag caaacacagc gcccaaaacc
     1441 gcaaactgca ccagattttc tgcatccaca agcacatttg ctcccagatc cagcataatc
     1501 gtatcactct ggcgcgtcgg aaaaatactg gcaatcgctg gacggtgaat tccgggcaaa
     1561 ggggtaagca ccatcttcgc cagcgccatc aaagcccccg tattgcccgc agacacaaca
     1621 gccgtcgcgc gcccctcttt aacagcctca atcgctagac gcatcgaact ccccttgctc
     1681 ttccgcaaga cagccgaagg tttctcatta ctcagaattt tatgctccgt atgaacaacc
     1741 gtgctcacag ccttaagtgc agactctctg tccaaaagcg gcccaatctg cccctcatcc
     1801 ccaaacagta taaatccaag ctgcggatgc gccttcagcg tcaacaccgc ccctggaata
     1861 acactctcag ggccataatc gccgcccatg gcatccaatg ctataattcg cgaacgagac
     1921 aagaaaacaa tccttgatat tcagataaag caaaagccct catgaaaggg ctttccaatt
     1981 ttgacaatat ttgcagacaa aagccagccc tttaaagctc gaccctaatc gcgcgcttca
     2041 agtacctgct tacctttata agtaccggtc ttcaaatcga tatggtgacg gcgcttcggc
     2101 tcacccgtat tggcatcttc aacccaattc tcagttgata aagcatcatg cgcacggcgc
     2161 atattacgct tagactttgt tgtttttctt tttggaacag ccatagctct tatcctttta
     2221 agtaacttga gcgcgttttt tacagccttt gccctcccaa cgcaaggatt ttctttcgct
     2281 taggaacaag acttagcttt cactgccaga aatcttatct ttccaatctt ttaacgccgc
     2341 aaacggattt tttaccaaat catcttcctc taaagctccc attttccgct caagctcttc
     2401 aaattcaaca ccttctgcat gcggatacgg attaatagaa agagataaat attgcgttac
     2461 caactccccc agatcaattt tgccctcaat caaggcttcc ggatcatcct tctcatccaa
     2521 aatcggaagc tccccatgtc ctttttcgtt caacttctca tgacgcgcct tggtaataga
     2581 caccgccact tccgggtccg cgtaccaagc ctcaaaatca tcctcaatat ggctatgcac
     2641 cggctccaaa gtaactacac aagcctgcgt aacatcggcg cgaatctggc ctttgatgta
     2701 atagctcatc tccccggcat ggtgcgaaac ccgaatatca gcctccaaag catctacact
     2761 ctccacaccc aaacgctggc tcaatcgccc gcacgccaac gcatcagggg caatcgttaa
     2821 agtctcctca gccttgttca tcgcctccac atcaaacaaa tgcgaccact cagtctctaa
     2881 aattcttttc ggcgcttttg acatctcact tattccttct caaacgcaat tttacccgac
     2941 aaaattgcat ccatatcctg tatctttaaa aatgccagaa tttctaacgc ttttacctcc
     3001 attttggcaa tctttttctc gtctgcctcc accgcgctgc catacaaatt cttgcgcaac
     3061 gccgccgcca aatccccatc cgtcaaagcc tgttcataag catgcatccg cccgttaaat
     3121 gctttcatca tcttacgcat atgtttcggc acccccatat cgccaatccc catttcacgc
     3181 aatgtttgat ccatatcctc aaaacacaca tcaaacagcg cttggttaaa gtgcagagcc
     3241 tcatcgcgcc cttcctcaag catccgctgg aaaaccaaaa aaatgaacag caccatcaga
     3301 tcaaaccgtc cgtcaaacgt atctggaacg ccgtattttt cataaaattt aggctgacgc
     3361 acacaaacca agacattcgc atacacatcg cgagtcattt gtttacatgg atttcttctc
     3421 attaaaaggt gaagcatttc agcaagcata agttttcttt tttttcagcg cgcactatag
     3481 tataaatctc cacccatgaa acataaaaag atacagccat gattcttcaa atatcgaaaa
     3541 aaacgcttct cctttcagct gcgattctaa cacttggaac aagcgcctgc acccccatgg
     3601 tcgcccagcg cggcaacatg gtcgaagatt ttcagatgca gcaaatcacg ccgcacgaaa
     3661 gcacacgctc cgatgtcttg cgcaatctcg gctcaccaac cagcaagtca acattcgatc
     3721 ccaatgtctg gtattacatc ggtcagaaaa ccgaaaaacg aggcattctc gaccctcagg
     3781 ttgtggaaga acggatcatc ttggtcgcct ttaatgagga aggctttgtc gaagccattc
     3841 aggacatcga ccgcgagcgc atgaacatcc cttatgcacg agaaaaaacc ccaacccacg
     3901 gtaatgagac gactgttatg cagcaatttc tcgggaatct gggtaaattc aacccgcaaa
     3961 ctggcgagca ataatacaac aaaaaagccc cgccaaaccg gcgggacttt cttaaattct
     4021 gttataggct gaacgcttaa gaaccagtag cgaatttcgc cacaatcgct accagcaaaa
     4081 tcgccacgat attggcaatc ttgatcaacg gattcaccgc cggcccggcc gtatccttat
     4141 acggatcgcc aacagtatcg cccgtcacag ccgctttatg agcatcggag cccttgccgc
     4201 cataattacc gtcttcaata tatttcttgg cattgtccca cgcaccgcca ccggccgtca
     4261 tggaaattgc cacaaaaata ccggttacaa tcgtcccgag caacattgcc cccagacatt
     4321 ggaatgccgc agccatgtcg cccgtcacct tatagatgac aacccacaaa acagccggag
     4381 ccaatacagg taagagcgat ggcacaacca tttcaccaat cgccgctttg gtcagcaagt
     4441 ctacagccgt accatattcc ggcttggcct tgccttccat gatacccgga atctccttga
     4501 actgcctgcg cacctcaata accacagaag ccgccgcgcg ccccactgcc gtcatcgaca
     4561 gcgcactgaa caaatacggc aacaacccgc ccagaaacaa accgatcaca atatacggat
     4621 cagtcagatc aaaacgaatg tcggccagct ccggcagata atgcgcaatt tcttccgtat
     4681 aaccggcgaa aagcaccaac gcggccaaac cggcactgcc aatcgcatag ccctttgtta
     4741 cagccttcgt tgtattgccg accgcatcca acgcatccgt cgtattgcga acgccctcag
     4801 gcagatccgc catctcggca attccgcccg cattgtcagt caccggacca taggcatcaa
     4861 gcgccacaac catcccggcc agtgaaagca ttgaagttgc agaaatcgca atgccataca
     4921 atcccgccag atcataagca acgtaaatcc cgccacaaat caccagcacc ggccacgcag
     4981 ttgcctccat ggaaatcgcc aatccgcgga tc
//
LOCUS       DAOSWR010000145         4983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11351_length_4983_cov_6.050528, whole genome shotgun sequence.
ACCESSION   DAOSWR010000145 DAOSWR010000000
VERSION     DAOSWR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11351_length_4983_cov_6.050528"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2511
                     /locus_tag="PK513_09345"
     CDS             178..2511
                     /locus_tag="PK513_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOO82695.1"
                     /translation="MGFKRTSEGRIFFQTSEDEPGKSAQQDNQSPNAPKINAGNSQNF
                     ETPFNLQRKTATRPSPQQPAQASQPTQMQIIALLKTLNERLKITQGEREKMARELKIY
                     RQTIEELQTKADHSDRAYQELSEKISAGGNSAHAESLARDALQELEETRRLLLDLETK
                     ATRADHGVSALKKLQADQAEKMVVSINHAAALTKRVKDTEERQEQVSARVDDALSQQA
                     RLSRRIDKAVEERTRFLRKLERIEETVLQTRDSLNAKAMVLLTDQGIAAQNELNEHGA
                     AARFAPGEYAAQAHAAQKTAVQDRQNQTLQVIGVGLLVTAAVLGGWLVSELQKAEDTE
                     FTQAKISSVPAQTVTNQVGKPTNVANMDWRIEEDTSAFDQQSAPMDNMPAVNPEQNDI
                     GTLNLNDQAQVEALLEGSPDAVAAALNNIAPGDDVMPDNIIAPKIAVETQSVEEPLQK
                     STGLALEAEANAPSATAVEKAFEPLKAQDPRGMIKPDSALPDVVKNIEAQAFEGISEA
                     QHDLAAIYTAGHGGVKQDYKRAAFWFEQAARRGVANAAYNLGVLNHQGLGGTKNLKKA
                     IEWYNRAAALGHPEAQYNLGIAYIEGIGVPYDAEKAARYFQNAAENNIMEAAYNLGLI
                     YENGLLGQAEPDRALVWYKTAADHGSPEAKQALEQLAKTLNVTLDDVNDLADRMKVME
                     KSSVSTEEQAATQNASADVSNTQITTAQVQEYLMRAGLYPGPADGISGPLTQDAIRSY
                     QRMHNLNPDGTASENLLSHMLANVIGENKVEQGSRAQ"
     gene            complement(2515..3423)
                     /locus_tag="PK513_09350"
     CDS             complement(2515..3423)
                     /locus_tag="PK513_09350"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOO82696.1"
                     /translation="MSAQLQHLFEVFAPAKINLYLHVTGRQGDGYHTLDSLISFADIG
                     DKISLEPAESLSLEITGAFTRVFKGKDISAAPDSTNLVIKAAWALARLAGKSPVFKIT
                     LEKNLPLGGGIGGGSSDCAAAIWAMLEHWNLPHDLDGLDALMLSLGADVPVCFACQTT
                     RVRGIGDILDPAPVLPEIPIVLAHPGKACNTQDVFRRFAGGFAELLYLPRNISDAETL
                     CAFLSRTENALTRAAIQCVPEIQNVLNAFDVQRDCLLARMSGSGSTCFGLFDDENAAK
                     NAAAALAYENPDWWVKSGWLGMPARY"
     gene            complement(3420..>4983)
                     /locus_tag="PK513_09355"
     CDS             complement(3420..>4983)
                     /locus_tag="PK513_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO82697.1"
                     /translation="GRYMDYILKKEPDNPALLKRAMVLAIGAGDYDRAMTFAHNIIEN
                     YESTNALSLMFLTAEAFRKQDYNAAATYIQTMPEGGLSAFIMPMLYSWSSASLGHYDV
                     DDLQDNSIHLYHAILVSDFMDKKDNIKSMLKQALTLGELSPEDVERIADIYAHIGETK
                     SAAALYEQAMRIESENETLLKKKEAAEKGESPEMFVRVETPAQGLGEAFYDMARLLSH
                     DFSDESARVFARLALFLNPEDTHSRLLLADIAARNERYADAIALYKGIEPKDSDFLKA
                     RRSAADLLYEQGRSDDAIAELQLLVNDFNDLESLIQIGNIHRINENFKDAIKNYNMAI
                     NTLINTLGEVSEDYWYLYYVRGMALEQDGQWDKAEADLKAALNFQPDHPYVLNYLGYA
                     WADQGNNLDKALKMIESAVTLRPNDGYITDSLGWVYYRMGRYKDAVPHLERAVELMPY
                     DPTINDHLGDAYWKVGRTIEAEFQWIRAKNHSENEELILAIEQKLLEGLNDLKTVKQA
                     RSERNKSATEMQ"
BASE COUNT         1347 a         1315 c         1183 g         1138 t
ORIGIN      
        1 cttcggtcat agatcgtagc ttaactcact gtccgaattc ttggggccac ctctaagccc
       61 cctcttaatc tgtggatgag cagacatcgg cattgaattt atttgcctgt attttacaaa
      121 attcgctata gttcgattca tatacaaaaa acacttgagt aacggagcga ttccaccatg
      181 ggattcaaaa gaacatcgga agggcggatt ttttttcaga cctccgaaga cgagccgggg
      241 aaatctgcac agcaagacaa ccaatcccca aacgctccaa agatcaatgc gggtaattcg
      301 cagaactttg aaacaccttt taatttgcag cgcaaaaccg cgacacgtcc ttctccacag
      361 cagcctgctc aagccagcca gcctacgcaa atgcaaatca tcgctttgct caaaactctg
      421 aatgagcgat taaaaatcac ccagggtgaa cgcgaaaaaa tggcgcgtga gttgaaaatc
      481 taccgccaga cgatcgaaga gttgcaaaca aaagccgatc acagcgaccg tgcctatcaa
      541 gaactttccg aaaagatcag cgctggtggt aacagcgctc atgctgaaag tctcgcccgc
      601 gatgccctgc aagaacttga agaaacgcgg cgtttattgt tggatttaga aaccaaagcc
      661 acgcgcgcag atcacggtgt ctctgcgctg aaaaaactgc aggctgatca ggccgaaaaa
      721 atggtcgtga gcatcaatca tgctgccgcg ctgaccaaac gtgttaaaga cacagaagag
      781 cgtcaggaac aagtcagcgc ccgtgttgat gacgcactca gccagcaagc acggctctct
      841 cgcaggattg acaaagctgt agaagagcgt acgcgtttct tgcgcaaact tgaacgcatc
      901 gaagaaacag ttctgcaaac tcgagattcc ctgaacgcca aggcgatggt gctgttgacc
      961 gaccagggta tcgccgcgca aaacgagttg aatgaacacg gcgccgcagc ccggtttgct
     1021 cctggtgaat atgccgcgca ggcccatgcc gcacagaaaa cagccgtgca agaccgtcag
     1081 aaccagacgc tgcaagttat cggcgtcggc ctgcttgtta ctgcagccgt gctgggcgga
     1141 tggttggtca gtgagttaca aaaagcagaa gatacagagt ttacacaggc gaaaatttcc
     1201 agcgtacctg cacaaaccgt taccaatcag gttggcaagc caacaaatgt ggcaaacatg
     1261 gattggcgca ttgaggaaga tacctcggcc tttgatcagc aaagcgcacc gatggataat
     1321 atgcctgctg tcaatccgga gcaaaatgac attgggactc tgaatttaaa tgatcaggcc
     1381 caggtcgaag cgctgctgga aggcagcccc gatgcggtcg ctgcggcgtt gaacaacatt
     1441 gcgccgggcg acgatgtcat gccggataat atcattgctc ccaaaatcgc tgtcgaaacg
     1501 cagtcagtag aagaaccgct gcaaaaaagc accggtctcg cgctcgaagc tgaggcgaac
     1561 gcaccgagcg cgacggcggt ggaaaaggct tttgagccgt tgaaagccca ggacccgcgc
     1621 ggaatgatca agcctgattc tgcgctgccc gatgttgtaa aaaacattga agcccaggct
     1681 tttgaaggta tttccgaggc gcagcatgat ctggctgcca tctacacagc gggccatggc
     1741 ggcgtcaaac aggattacaa acgcgcagct ttctggttcg aacaagccgc tagacgcggc
     1801 gttgccaatg ctgcctacaa tctcggtgtg ctcaaccatc aaggtcttgg cggtacaaaa
     1861 aacctgaaaa aagccatcga atggtataat cgcgccgccg ctctcggtca tccggaagcg
     1921 caatataatc tcggcatcgc ttatatcgaa ggcataggtg tgccttatga tgcggaaaaa
     1981 gccgcgcgct atttccaaaa tgcggccgaa aacaacatca tggaagctgc ttacaatctg
     2041 ggcctgatct atgaaaatgg ccttttaggg caggccgaac ctgaccgtgc attggtctgg
     2101 tataaaaccg ctgccgatca cggaagccct gaggccaaac aagcgttgga acaactcgcc
     2161 aaaaccctga atgtgacgct cgatgatgtt aatgatctgg ctgatcggat gaaagtgatg
     2221 gaaaaatctt ctgtatcaac cgaagagcag gcagcgaccc aaaacgcatc tgcagacgtt
     2281 tcaaatacgc aaattaccac cgcacaggta caggaatatc tcatgcgcgc aggcctgtat
     2341 cccggtcccg ccgatggaat cagcggcccg cttacgcaag atgctatccg gtcttaccaa
     2401 cgtatgcata atttgaatcc cgatggcacg gccagtgaaa acctgctcag ccatatgctg
     2461 gccaatgtca ttggcgaaaa caaggttgag caaggctctc gcgcgcaata gatattaata
     2521 ccgtgcgggc attccaagcc agccggattt cacccaccag tctggatttt cgtaagcaag
     2581 cgccgctgcc gcattttttg ccgcattctc atcgtcaaac aatccaaaac atgtcgaacc
     2641 tgatccgctc atccgtgcga gcaggcaatc tctttgcaca tcgaaagcgt ttaaaacatt
     2701 ttgtatctct ggcacacatt gaatagccgc gcgggtgagt gcattttccg ttctgctcag
     2761 aaaagcacac agggtttctg cgtctgaaat attgcggggc aggtagagca attcggcaaa
     2821 gccaccggca aaacgtctaa aaacgtcctg cgtattacaa gccttacccg gatgtgcaag
     2881 cacaataggt atttccggca aaacgggtgc aggatcgaga atgtcgccaa tgccacgcac
     2941 acgcgtggtt tgacaggcaa aacagaccgg cacatctgcc cccagagata acatgagcgc
     3001 atccagcccg tccagatcat gcggcaaatt ccaatgctcc agcatcgccc agatcgccgc
     3061 cgcgcaatca cttgagccgc cgccaattcc tccgcccaaa ggcagatttt tctccaaagt
     3121 aattttgaaa accggacttt tcccagccaa tcgtgccaac gcccacgcag ccttgatcac
     3181 taaattggta gagtccggcg ccgcgctaat gtctttgcct ttaaagacgc gtgtaaacgc
     3241 tcctgtgatt tcaagtgaca agctctcggc aggctcaagg ctaatcttgt cgccaatatc
     3301 cgcaaaagaa ataagtgaat ccagcgtatg ataaccatcg ccctgccgac cagtgacatg
     3361 caggtaaaga tttatttttg caggcgcaaa aacctcaaaa aggtgctgca actgcgcgct
     3421 cattgcattt ctgtagcgct tttattgcgc tctgaacggg cttgcttcac agttttaaga
     3481 tcgtttaagc cttctaaaag tttttgctca attgctaaaa taagctcttc attttcggaa
     3541 tggtttttcg cgcgaatcca ttgaaactca gcttcgatcg tacgcccgac tttccaatag
     3601 gcatcgccca gatgatcgtt aatggtcgga tcataaggca tcagctcaac ggcacgctcc
     3661 agatgcggta cggcatcttt atagcgtccc atccggtaat acacccagcc caatgagtcg
     3721 gtgatatagc catcattggg acgcaaagtt acggcgctct caatcatttt cagcgccttg
     3781 tccagattgt tgccctgatc cgcccacgca tagccgagat aattcagaac ataaggatga
     3841 tcgggctgaa aattaagtgc ggcttttaaa tccgcttccg ccttgtccca ctgcccgtcc
     3901 tgctcaaggg ccatgccgcg cacgtaataa agataccagt aatcttcact cacttcgccc
     3961 agggtattga tgagcgtatt aatggccata ttataatttt tgatcgcgtc tttaaaattt
     4021 tcattgatgc gatggatgtt gccaatttga ataagagatt caagatcatt aaaatcattc
     4081 accagaagtt gtagctctgc aattgcatcg tcgctccgcc cttgttcata aagcaagtcg
     4141 gcagcgctgc gccttgcttt aagaaaatcg ctatccttag gctctatacc tttataaaga
     4201 gcaatcgcat cagcgtatcg ttcgttgcgc gcggctatat cagcaagcaa taaacgcgag
     4261 tgagtgtctt cgggatttaa aaagagggcc aaacgagcaa aaacacgtgc gctttcatca
     4321 ctgaaatcat gagaaagcag gcgtgccata tcataaaatg cttctcccaa accttgcgcc
     4381 ggggtttcca cgcgcacaaa catttcggga ctctcgcctt tttccgcagc ctctttcttt
     4441 ttaagaagcg tctcattttc cgactcaatt ctcatcgcct gctcataaag tgcagcggcg
     4501 ctcttggtct caccaatatg ggcataaata tcggcaatgc gttcaacatc ctcaggacta
     4561 agctcgccaa gggtcaaagc ctgttttagc atgcttttga tgttatcttt tttgtccata
     4621 aagtcggata ctaaaatggc atgatacaga tgaatgctat tatcctgcag atcgtcaacg
     4681 tcataatggc caagcgatgc gctggaccag ctatagagca tcggcatgat aaaggcggac
     4741 aggccacctt caggcatcgt ctggatataa gttgccgccg cattataatc ttgcttgcgg
     4801 aacgcttcgg ctgtcagaaa catcagcgac aacgcattgg tgctttcgta attctcgatg
     4861 atattatggg caaacgtcat tgcccggtca taatctccgg ccccgatcgc cagcaccatt
     4921 gcgcgtttga gcagcgcggg attgtccggc tctttcttca gaatgtagtc catgtagcgc
     4981 ccg
//
LOCUS       DAOSWR010000146         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11380_length_4976_cov_5.193253, whole genome shotgun sequence.
ACCESSION   DAOSWR010000146 DAOSWR010000000
VERSION     DAOSWR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11380_length_4976_cov_5.193253"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK513_09360"
     CDS             complement(<1..205)
                     /locus_tag="PK513_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82698.1"
                     /translation="MCKDKKTGEITIHSAVSPDEIKDQADENVLIGLFPHVWPEDWKS
                     KLDNKMEDTHKTYIELIREQIEKA"
     gene            330..1646
                     /gene="eno"
                     /locus_tag="PK513_09365"
     CDS             330..1646
                     /gene="eno"
                     /locus_tag="PK513_09365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO82699.1"
                     /translation="MTAIIDIHAREILDSRGNPTVEVDVQLESGAKGRAAVPSGASTG
                     AYEAVELRDGDKGRYLGKGVRKAVEFVNTEIYEALIGMDGEEQMYIDQAMVELDGTGN
                     KGRLGANAILGVSLAVAKAMADELDMPLYRYVGGAYAHTLPTPMMNIINGGAHADNPV
                     DIQEFMIMPIAAESFSEALRAGAEVFYALKKELSSAGLNTNVGDEGGFAPAVKSSEEA
                     LDYIMKSIKAAGYKPGEEITIALDAASSEFYKDGQYHLAGEGKVLSSDEMVRYYEDLC
                     AKYPIVSIEDGLDEDDWQGWSALTAALGDKVQLVGDDLYVTNASRLARGIDEKAGNAV
                     LVKVNQIGTLSETLETIEMAKKAGFGIVLSHRSGETEDSTIADLAVATNAGQIKTGSL
                     SRSDRVAKYNQLLRIEEELGPQAAYEGAGFLRAPVSFGAKAKKNAA"
     gene            1789..2100
                     /locus_tag="PK513_09370"
     CDS             1789..2100
                     /locus_tag="PK513_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOO82700.1"
                     /translation="MNKLSDHSYFLRENILPLIGVCLCLYFSYHTLLGNRSVLKLYAL
                     EKQIETMSQKKTFTVAAKDSLQKKVIMMRPGNVDKDLLEEQVRLVLGYRQSDELVFLG
                     N"
     gene            2334..3284
                     /gene="pdhA"
                     /locus_tag="PK513_09375"
     CDS             2334..3284
                     /gene="pdhA"
                     /locus_tag="PK513_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOO82701.1"
                     /translation="MLQKLYREMLLIRRFEEKAGQLYGMGLIGGFCHLYIGQEAVVTG
                     INSVQQPQDTVVTSYRDHAHMLACGMDPKGIMAELTGRIDGYSRGKGGSMHMFSKAEN
                     FFGGHGIVGAGTAIGAGLGFSHKYKEDDGVAVAYMGDGASNQGQVAEVYNMAALWDLP
                     VLFVIENNQYGMGTSVKRSSAGQLYKRGEGYGIPGEQVDGMDVFAVQKAAKQALDYVR
                     SGNGPYILEVMTYRYRGHSMSDPAKYRTKEEVASMKEDRDPITALKGLMQAAGVDEAE
                     IKAIDKEIKDIVNEAAQFAQDSPEPAPEELWTDVLVEVDE"
     gene            3580..>4976
                     /locus_tag="PK513_09380"
     CDS             3580..>4976
                     /locus_tag="PK513_09380"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOO82702.1"
                     /translation="MSINILMPALSPTMTEGNLAKWLKSEGDKVEAGDVIAEIETDKA
                     TMEVEAVDEGVLGKILVAEGTENVSVNDVIAVLLEEGESADDVDASSLRAEGEAIQSK
                     GQADEEGGLPCRARAPRNDAQQTEGKVIENRDILYAQGAVLEPSPFDEASFTEIQDMT
                     VREALRDGMAEEMRADVNVYLMGEEVAEYQGAYKVSQGLLDEFGAKRVIDTPITEHGF
                     AGMAVGSAMNGLKPIVEFMTWNFGMQAIDHIINSAGKTLYMAGGQLGCPIVFRGPNGA
                     ASRVGAQHSQCYASWYAHVPGLKVVAPWSGADAKGLMKAAIRDPNPVLILENELLYGE
                     THSVPVSDEFVIPLGRAKIERSGSDVTIVAFSIMVGKALKAAEKLAEQGIDAEVINLR
                     TIRPLDRYTILESVKKTNRIVTCEEGWPFAGIGAEIAALIGEHAFDYPDAPLARVAAA
                     DVPLPYAANLEALALP"
BASE COUNT         1147 a          948 c         1547 g         1334 t
ORIGIN      
        1 gggctttttc aatttgttct ctgatcaatt ctatataggt tttatgtgtg tcttccatct
       61 tgttatccag tttagatttc cagtcctctg gccatacatg cgggaacaga ccgattagga
      121 cattttcatc tgcttgatcc tttatttcat ctggtgatac tgccgaatgt attgttattt
      181 cccctgtttt tttatctttg cacataattt ccgtattgta tttagtcaag tttttttgac
      241 attgttccga ttgacaggtt cgtcgtcgcg tttgtattgt cccgaaagaa aaacgcgtaa
      301 tgtctgaccc taataaggag tgcctttcta tgactgccat tattgatatt catgctcgtg
      361 aaattctgga tagccggggg aacccgactg tggaggttga tgtgcagctt gaaagcggcg
      421 cgaagggccg ggcggcagtg ccgtcggggg cttctacggg ggcgtatgag gcggtggagt
      481 tgcgtgatgg agataagggc cgttatctcg gtaagggtgt gcgcaaggct gtcgaatttg
      541 taaataccga gatttatgag gcgctgattg gtatggacgg cgaagagcag atgtatatcg
      601 atcaggcgat ggtcgaactg gacgggactg gaaataaggg ccgcttgggg gccaatgcga
      661 ttttgggcgt gtcgctggcg gtggccaaag cgatggcgga cgagctggat atgccgcttt
      721 atcgttatgt tggcggggcg tacgcgcata cgctgccgac gccgatgatg aatatcatca
      781 atggcggggc gcatgcggat aacccggttg atattcagga atttatgatt atgccgattg
      841 cggcggagag cttttctgaa gctttgcgtg caggcgcgga ggtgttttat gcgctgaaga
      901 aagaattatc atctgccgga ttaaatacca atgttggtga tgagggcggg tttgcgcctg
      961 ctgtgaagtc atctgaggaa gcgctggatt atattatgaa gtcgattaaa gcggctggct
     1021 ataagccggg cgaagagatt acgattgcgc tggatgcggc ttcgagcgag ttttataagg
     1081 atggtcaata tcatctggct ggcgagggga aggttttgtc gtcggatgaa atggtgcgct
     1141 attacgaaga tctgtgcgcg aaatacccga ttgtttcgat tgaagatggt ttggatgaag
     1201 atgattggca ggggtggagt gcgcttacag ccgctcttgg tgataaagtg cagcttgtcg
     1261 gcgatgatct gtatgtgacc aatgcaagcc gtttggcacg cgggattgac gagaaagcgg
     1321 gtaatgctgt gctggtgaag gtcaatcaga ttgggacgtt gagcgagact ttggaaacca
     1381 ttgagatggc gaagaaagcc ggtttcggga ttgttctgtc gcaccgcagc ggcgaaacag
     1441 aggattctac gattgctgat ctggcggtgg caaccaatgc cgggcagatc aaaacgggtt
     1501 ctttgtcgcg ttcagaccgg gtggcgaaat ataaccagct gctacggatt gaagaagagc
     1561 tggggccgca ggcagcttat gaaggggcag gattcttgcg tgcgccggtg tctttcggcg
     1621 cgaaggcaaa gaaaaacgcg gcttaaaaaa cataaaaagt ttatgtgcta aaatgtgagt
     1681 catttgaatc gtttagcgca tatgttcttt ttttgttcac attaaattgt tgaatctatt
     1741 gtattttttg ccttgttcac agactcggaa tgtgattcac tcaaggacat gaataagctt
     1801 agcgaccata gttatttctt acgtgaaaac attttacctc ttatcggggt gtgtttgtgt
     1861 ttgtattttt cttatcatac gcttttgggt aatcgttcgg tgttgaagct ttacgcgttg
     1921 gagaagcaaa tagagacaat gtcgcaaaaa aagactttta cggtggcggc gaaagattct
     1981 ttgcagaaaa aagtgattat gatgcgtcca ggcaatgttg ataaagattt actggaagag
     2041 caggttaggc tggtgctggg ttacagacag tccgacgaat tggtgttctt aggtaattaa
     2101 attacaaaaa ttcctgtttt ttatttttta tcctttcttt tcagtatgtt gcgccgcaca
     2161 aatatgctat ttttgctggt cgcctcttgt gaaatcgtgt aattcatctg tcttgatttc
     2221 aacagttctg ttctttcaat gatggaggcg ccaaaatcgt ggccagttca aagaccaaaa
     2281 agaaacctaa gctcaaaact gtatcgaaca atgagtcaat tccgtctgtg gagatgttac
     2341 aaaagctcta tcgcgagatg cttttgattc gccggtttga agaaaaggct gggcagcttt
     2401 acgggatggg cttgattggc gggttttgtc acctttatat cggtcaggaa gccgttgtga
     2461 cgggaattaa ttctgtgcag cagccgcaag atacggtggt gacgtcgtat cgcgatcatg
     2521 cacatatgct ggcgtgcggg atggatccga agggcattat ggccgagctg acgggacgca
     2581 ttgacgggta ttcgcgcggg aaaggcggtt ccatgcatat gttttcgaag gcagagaatt
     2641 tttttggcgg gcacgggatt gtcggggcag gcacggcgat tggcgcgggg cttggctttt
     2701 cgcataaata taaagaagat gacggcgtgg cggtggccta tatgggggat ggcgcgtcga
     2761 atcaggggca ggtggctgag gtttataaca tggccgctct gtgggatttg ccggtgttgt
     2821 ttgtgattga gaataatcag tatggcatgg gtacatctgt gaagcgttct tccgccgggc
     2881 agttgtataa acgcggcgaa ggttacggca ttcccggtga gcaggtagac gggatggatg
     2941 tgtttgctgt gcagaaggcg gcgaagcagg cgcttgatta tgtgcgcagc ggcaacgggc
     3001 cgtatattct tgaggtgatg acatatcgtt atcgcgggca ttcgatgtct gatccggcga
     3061 aataccggac caaggaagag gtggcctcaa tgaaggagga tcgcgatccg attacggcgc
     3121 ttaaggggct gatgcaggct gcgggcgtgg atgaagccga gatcaaggca atcgacaagg
     3181 agatcaagga tatcgtcaat gaagcggcgc aattcgcgca ggacagtcca gagcctgcgc
     3241 cggaagagtt gtggacggat gttttggtgg aggtggacga gtgatgtttt atctttgtat
     3301 gccgataaat tctctctgtt ggtctggtct tagtcctgcc gtgccaccac ttttgattgc
     3361 acgttttacg cgtcgatctt tggtagatac ttccagaaag gatgctagtc tttggggcgt
     3421 acaactattg aaggcgttta aaaagcactt tttccagtat cttcccgatt gggtttgctg
     3481 tctgaattct cttttaattt caaaaaagag gtcatcacca ttcactgtac gcataactat
     3541 aaacccctca aaacgatttc gtaacaaagg cttttaatta tgtcaataaa tattttgatg
     3601 ccggcgctgt cgccgacgat gacggaagga aatcttgcca agtggctgaa gtccgagggc
     3661 gataaggttg aagctggcga cgtgattgcc gagattgaga ccgacaaggc gacgatggag
     3721 gttgaagccg tggatgaagg tgtgctgggc aaaattcttg tggctgaagg caccgaaaat
     3781 gtgtcggtga atgatgtgat tgctgtactt ctggaagagg gtgagagcgc tgatgatgtt
     3841 gatgcatcgt cattgcgagc cgaaggcgaa gcaatccagt ctaaagggca agcagatgaa
     3901 gaaggtggat tgccgtgtcg tgcgagagct cctcgcaatg acgctcagca aacagaaggc
     3961 aaagtaattg aaaaccgcga tattttgtat gcgcaagggg ctgtgctgga gccgtcgccg
     4021 tttgatgaag cctcttttac tgaaattcag gatatgacgg tgcgtgaggc gttgcgcgac
     4081 ggtatggccg aggaaatgcg cgccgatgta aatgtctatc tgatgggtga ggaggtcgcc
     4141 gaatatcagg gtgcgtataa ggtaagccaa gggcttttgg atgaatttgg tgccaagcgc
     4201 gtgattgata cgccgattac cgagcatgga tttgccggga tggctgtggg atctgcgatg
     4261 aacggtctga agccaattgt ggaattcatg acctggaact ttgggatgca ggcgatcgac
     4321 catatcatca actctgccgg gaagacactg tatatggccg gtgggcaatt gggctgtccg
     4381 attgtgtttc gtgggccaaa cggggcggcg agccgggtcg gggcgcagca ttcgcaatgt
     4441 tatgcgagtt ggtatgcgca tgtgccggga ttgaaagttg ttgcgccttg gtcgggggcg
     4501 gatgccaagg gcctgatgaa ggcggcgatt cgtgatccga acccagtgct gatccttgag
     4561 aatgaattgc tgtacgggga aacgcacagt gtgccggtga gtgatgagtt tgttattccg
     4621 ctggggcggg cgaagattga gcgcagcggt tcggatgtca cgatcgtggc gttttcgatt
     4681 atggtgggca aggcgctcaa ggcggcagaa aagctggctg agcaggggat tgatgccgag
     4741 gtgatcaatt tgcgcacgat ccgcccgctg gatcgctata cgattttgga aagcgtgaag
     4801 aagactaacc gtattgtaac ctgcgaggaa ggctggccgt ttgcgggaat aggcgcggaa
     4861 attgcggcgc tgatcggcga gcatgcgttc gattatcccg atgcgccgct ggcgcgcgtg
     4921 gcggcggcgg atgtgccgct cccctatgcg gcgaatttgg aggctttggc cctgcc
//
LOCUS       DAOSWR010000147         4955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11446_length_4955_cov_5.038163, whole genome shotgun sequence.
ACCESSION   DAOSWR010000147 DAOSWR010000000
VERSION     DAOSWR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11446_length_4955_cov_5.038163"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK513_09385"
     CDS             complement(<1..329)
                     /locus_tag="PK513_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOO82703.1"
                     /translation="MENIKYYLSWALWPALFVICMSVAGYGFSHDAPVLYFNIAYVFL
                     ILSLFFLEKWMPYEREWLKPDGQNTASILHTISSKGSVQLLFLFSGVIGLADYITPMD
                     QEGYSVWP"
     gene            complement(418..1458)
                     /locus_tag="PK513_09390"
     CDS             complement(418..1458)
                     /locus_tag="PK513_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOO82704.1"
                     /translation="MKKILVTGATGFIGHAFTKELLATGHKVLGVDNLNDYYDVALKH
                     ARLAELEGQSGFTFQQLDLADREAMTALFEAEKPQIVVNLAAQAGVRYSLDHPHAYVD
                     SNLQGFVNVLEGCRHSGVEHLIFASSSSVYGANTKVPFAVSDNVDHPISLYAATKKAG
                     ELMAHSYAHLYGIPCTGLRFFTVYGPWGRPDMAYFKFTKAIYEGKPIDVYNDGDMMRD
                     FTYIDDITAAMMKLLDHVPEQDASWDSKKPDPSFSNAPYRLYNIGHNHPEQLMDMIHI
                     LEAETGQHAEKNFMPMQPGDVYATYADVKGLEEVIGFKPETSLAEGLKRFVDWYKSYY
                     SIGDEAAASLRA"
     gene            complement(1471..2433)
                     /locus_tag="PK513_09395"
     CDS             complement(1471..2433)
                     /locus_tag="PK513_09395"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOO82705.1"
                     /translation="MIKSANPESIAEAAEILKSGGLVGMPTETVYGLAADATNGQAVA
                     KIFAAKGRPSFNPLIIHVTDLEQAGEFVVVNDWARAMSQAFWPGPLTLILPKKENTQI
                     SELASTGLETLAVRVPAHKVARALIKAACVPLAAPSANASGTLSPTTAAHVYDSLGDK
                     VDIILAAGACAVGLESTVLDLSGEAPLVLRPGGISAEDISKVLGVEVRYDAGDSVAPK
                     SPGQLLKHYAPNLPVRLNAIDLKEDEALLAFGRTKFMIGSDFPAELVCNLSENKDLYE
                     AAANLFAMLKVLDESGAKGIAVMAVPEVGIGIAINDRLRRAAQA"
     gene            complement(2430..3515)
                     /locus_tag="PK513_09400"
     CDS             complement(2430..3515)
                     /locus_tag="PK513_09400"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOO82706.1"
                     /translation="MSEHKVYRAIGLMSGTALDGEIDVALVETDGHAFVKPLGFYAHP
                     YDLSVRDNVRACFGKRVPDAQTREAEALVTDLHIAAVKASGFEADVIGFHGQTITHAP
                     KDSFTWQLGDGAWLAVETGMDVVNDFRSADMKAGGQGAPLTPLYHAAIMVGQDKPVAV
                     LNLGGVANVTYIAEDGDLCAFDCGPGNALMDDFIKHRRGVAFDKDGMLASKSLPHVGI
                     LEGFLADDYFSLPGPKSLDRNSWSVDIVRDLSDEMGMATLFAMSVGAIEKSVKGLPNV
                     PKVIYVAGGGRKNKYLMEVLSKRLGGFWRPKSKMKSIDDVGYDGDAIEAECFGYLAVR
                     SLLGLPLSLPETTGIARPVSGGVLHKA"
     gene            3534..4829
                     /gene="tyrS"
                     /locus_tag="PK513_09405"
     CDS             3534..4829
                     /gene="tyrS"
                     /locus_tag="PK513_09405"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO82707.1"
                     /translation="MTKYKSEFLNILTERDFIHQCSDFNGLDAKLSKGVQSAYIGFDA
                     TADSLHVGSLVQIMMLYWFQQCGHKPITLMGGGTTRVGDPSGKDESRRMLTETDIEKN
                     IGGIGLIFGNYLRYGSGRTDALMVNNADWLDQLNYIDFLRDYGPHFTINRMMGFESVK
                     LRLEREQPLTFLEFNYMILQAYDFVELRQRFGTILQMGGSDQWGNIINGVELWRRTSP
                     REKSKGFSVGNYSGPATDPVFALTTPLLTTASGAKMGKTADGAVWLNADKLPAYDFWQ
                     YWRNTEDADVGRFLKLFTTLPMDEIARLEAVQGAEINEVKKVLAFEVTKLCHGETAAR
                     EAADTAAKVFEQGSIGADLPCVQLDLSAGVTFIDAACAAGLTTSKGEARRIISQGGAK
                     VNGQPVTDPAFTLTADMISEGDAIKISTSKKKHALVKAS"
BASE COUNT         1318 a         1571 c         1003 g         1063 t
ORIGIN      
        1 ggccagaccg agtacccttc ctgatccatc ggcgttatat aatcggccag cccgatcacc
       61 ccgctaaaca ggaagagtaa ctgcacactg cctttcgagc tgatcgtatg caggatcgag
      121 gccgtattct gcccatcggg tttcagccat tcgcgctcat acggcatcca tttttccaga
      181 aaaaacagcg acaaaatcaa aaacacatag gcaatattga aatacagcac tggcgcatca
      241 tgcgaaaagc cgtatcccgc cacgctcata caaatgacaa acaatgccgg ccacaaagcc
      301 caggataaat aatatttaat gttttccatg ctcaatctcc atttaatgtc attgcgagga
      361 gtaagaccga cgcaacaatc catccctgaa acaattacaa tagatggatt gcttcgctca
      421 cgctcgcaat gacgcagcgg cctcatcgcc gatgctgtaa taacttttat accaatcgac
      481 aaaccgcttt aacccctcgg ccaaagacgt ttccggttta aaaccgatca cctcttccag
      541 ccccttcaca tcggcatagg tcgcataaac atcaccgggc tgcatcggca taaaattctt
      601 ctcggcgtgt tgtcctgttt ctgcttccaa aatatggatc atatccatca attgctcagg
      661 atgattatgt ccaatattat ataaacgata cggcgcgtta ctaaatgatg ggtctggttt
      721 ttttgaatcc caacttgcgt cttgttcagg cacatgatca agcagcttca tcatcgctgc
      781 ggtaatatca tcaatatagg tgaaatcgcg catcatatcg ccgtcattat aaacatcgat
      841 cggcttgccc tcataaatcg ccttcgtaaa tttaaaatac gccatatccg gacgccccca
      901 cggcccgtac acggtaaaaa accgcaaacc cgtacacggg atgccgtaaa gatgcgcata
      961 ggaatgcgcc attaactcac ctgctttctt tgtcgccgca tacaaactga tcggatgatc
     1021 gacattgtca gaaaccgcga acggcacctt cgtattcgcc ccgtaaaccg aagacgatga
     1081 agcaaaaatc aaatgctcca caccgctgtg gcggcagccc tccagcacat tcacaaaccc
     1141 ctgcaaattc gaatcgacat aggcatgcgg atgatcgagc gaataacgca caccggcttg
     1201 ggccgccaga ttgacaacaa tctgcggctt ttccgcctca aacaacgccg tcatagcctc
     1261 acgatccgcc agatcaagct gctgaaaggt aaaaccgctt tgcccctcaa gttcggccaa
     1321 tcgcgcatgt ttcagcgcaa catcgtaata atcattcaga ttatcaaccc cgagcacttt
     1381 atgcccagtc gccagcaatt ctttggtaaa ggcatgccca atgaaaccag tcgcccctgt
     1441 cacaagtatc tttttcattg gcctatccta ttacgcttgc gccgcccgcc gcaagcgatc
     1501 attaatcgca ataccgatcc cgacttccgg cacggccatc acagcaatcc ccttcgctcc
     1561 gctctcgtca agcactttca gcatcgcaaa caaattcgcc gccgcttcat acaaatcctt
     1621 attttcactc aaattacaaa ccagttccgc cggaaaatca ctcccaatca taaattttgt
     1681 tctgccaaaa gcgagcagcg cctcgtcctc tttcaaatca atcgcattta accgcaccgg
     1741 caaattcggc gcataatgct tcagtaattg ccccggcgac tttggcgcca cagaatcgcc
     1801 tgcatcatac cgaacttcaa caccaagaac tttggatata tcctccgcac tgatcccgcc
     1861 cggacgcaaa actagcggcg cttcaccgct caaatccagc accgtggatt ccagccccac
     1921 cgcgcacgcc ccggccgcca aaataatatc caccttatcc cccagcgaat catacacatg
     1981 cgccgccgtt gtcggactaa gcgttcccga cgcattcgca ctcggcgccg ccaagggtac
     2041 acatgccgcc tttatcaacg cccgtgccac cttatgcgca ggcacgcgca ccgccagcgt
     2101 ttccagcccc gtactcgcca gttcggaaat ctgcgtattc tctttctttg gtaaaatcaa
     2161 cgtcagcggc cccggccaga aagcttgcga catcgcgcgc gcccagtcat taaccacaac
     2221 aaattccccc gcctgctcca gatccgtgac atgaatgatc aagggattaa agctcggccg
     2281 tcctttggcc gcaaaaatct tcgccacagc ctgcccattg gtcgcatccg ccgccagacc
     2341 gtaaacagtc tcagtcggca taccgaccag cccgccggat ttcaaaatct cggcagcctc
     2401 ggcaatactt tcaggatttg cgctcttgat catgctttgt gcaatacacc accggatacg
     2461 ggccgtgcaa tccccgtcgt ttcgggcaaa ctcagcggta aacccaacaa agagcgtacc
     2521 gccagataac caaaacactc cgcctcaatt gcatcaccgt cataaccaac atcatctata
     2581 gatttcattt tacttttagg cctccaaaaa ccacctaaac gcttggataa aacctccatc
     2641 aaatatttat tttttcgtcc gccaccagcg acataaataa ccttgggaac atttggcaac
     2701 cccttcactg atttttcaat agcccccacg ctcatagcaa acaacgtcgc catccccatt
     2761 tcatctgaca agtcacgaac aatatcaaca ctccaactat tacggtccaa cgatttggga
     2821 cccggcaaac taaaatagtc atcggcaaga aaaccctcaa gaatacccac atgtggcaag
     2881 ctttttgagg caagcatacc atccttatca aaggcaacgc ctcgcctgtg cttaataaaa
     2941 tcatccatca acgcattccc cggcccgcag tcaaacgcac ataaatcgcc atcttccgca
     3001 atatacgtca cattcgccac accacccaga ttcaaaactg ccaccggctt atcctgacct
     3061 accataatcg ccgcatgata cagcggcgtc aatggcgcac cctgcccccc ggctttcata
     3121 tccgctgaac gaaaatcatt caccacatcc atacccgtct ccaccgccag ccatgctccg
     3181 tcaccgagct gccatgtaaa actatccttc ggagcatggg taatggtctg gccgtgaaag
     3241 ccaatcacat ccgcctcaaa accgcttgcc ttcaccgccg caatgtgcaa atccgtcacc
     3301 agagcttccg cttcacgcgt ctgcgcatcg ggcacgcgtt taccaaaaca cgcccgcaca
     3361 ttatcccgca cgctcaaatc ataaggatgc gcataaaacc ccagcggttt aacgaaagcg
     3421 tgcccgtcgg tttccactag cgcgacatca atctccccgt ctagcgccgt tccggacatc
     3481 aggccaattg ccctatagac tttatgctca ctcatgctta tacaatttgg gttatgacga
     3541 aatacaaatc agaattttta aacatcttaa ccgagcgcga cttcattcac cagtgctctg
     3601 atttcaacgg ccttgatgcc aagctgagta aaggcgtgca aagcgcatat atcggctttg
     3661 acgccaccgc tgactccttg catgtcggtt ccctcgtcca aatcatgatg ctttactggt
     3721 tccagcaatg cggccacaag ccgatcaccc tgatgggcgg cggcaccacc agggtcggcg
     3781 acccttcggg caaggatgaa agccgccgga tgctcactga aaccgatatc gaaaaaaaca
     3841 tcggcggcat cgggctgatc ttcggaaact acctccgata cggcagcggc cgaaccgacg
     3901 cgctgatggt gaataacgcc gactggcttg accagctcaa ctatatcgac ttcctgcgcg
     3961 attacggccc gcactttaca atcaaccgta tgatgggctt cgagtccgtc aaactccgcc
     4021 ttgagcgaga acaaccgctg accttcctcg agtttaacta catgatcttg caggcttacg
     4081 actttgtcga attacgccag cgcttcggca ccattttgca aatgggcgga agcgaccagt
     4141 ggggaaacat tatcaatggc gttgaattat ggcgacgaac ctcgccaaga gaaaaaagca
     4201 aaggtttcag cgttggcaat tactctggac cagctacaga tccagttttc gccctcacca
     4261 ctccgctgct taccaccgcc agcggcgcaa aaatgggcaa aaccgccgat ggcgcggtct
     4321 ggctcaacgc cgacaagctc cccgcatacg acttctggca atactggcgc aataccgaag
     4381 acgccgatgt cgggcgtttc ctcaagctct tcaccacctt gccgatggac gaaatcgccc
     4441 gcctcgaagc cgtgcagggc gcagaaatca acgaggtcaa aaaagtactg gccttcgaag
     4501 tcaccaaact ctgccacggc gaaaccgccg cacgcgaagc cgccgacacg gctgccaaag
     4561 tcttcgaaca aggctcgatc ggcgcggatt taccctgcgt tcaactcgac ctctccgcag
     4621 gcgtaacctt catcgatgcc gcctgcgccg ccggactcac cacatcgaaa ggcgaagccc
     4681 ggcgcatcat cagtcaaggc ggcgcgaaag taaacggaca gcccgtcacc gatcccgcct
     4741 ttaccctcac cgccgatatg atcagcgaag gcgatgccat caaaatctca acttcgaaga
     4801 aaaaacacgc actggtaaaa gcgagctaaa aaatttattt ctgctcgaac agcacgcgcc
     4861 gtaaaattcc tggcgtcaga actgacagcg ggtttaccga aacttttgga tcctcgcttg
     4921 atcccttcat cgtataggtc gccgcgatca gagcg
//
LOCUS       DAOSWR010000148         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11529_length_4929_cov_5.366434, whole genome shotgun sequence.
ACCESSION   DAOSWR010000148 DAOSWR010000000
VERSION     DAOSWR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11529_length_4929_cov_5.366434"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..379)
                     /locus_tag="PK513_09410"
     CDS             complement(68..379)
                     /locus_tag="PK513_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO82708.1"
                     /translation="MKIYVTDQNGVEHALDAVEGWRVMEIIRDHGLPIKAECGGACAC
                     ATCHVYVDENWLSKLAMMRDDEEEMLDEAFDVQDNSRLSCQIIMDESLDGLKVRLACY
                     D"
     gene            complement(389..1189)
                     /locus_tag="PK513_09415"
     CDS             complement(389..1189)
                     /locus_tag="PK513_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82709.1"
                     /translation="MASFDFVESTSQAYRFVWNRRVDVVRFSAMVLVLKILFFVGFVA
                     FDIQKEALRQGLLLLPIFFMEGWVIATLVIMALHAHETRRETRSSILPPAEDTARNIK
                     ASMIVYVLIKLMLSFVVGSAYHGQQTIPDAPPPDPSLQTFILAMMLIVFLIWAFRFLW
                     IYIPVVMGKSVRTYLIRFRAYSDSFPLLGVWVLCFVPMMLFMILLSEFYGTVMGVLGV
                     GDSSIVFETGMAVIQAFIDFVLSLVSSLAVAYGVYSVFNNENKKTDIW"
     gene            1286..2320
                     /locus_tag="PK513_09420"
     CDS             1286..2320
                     /locus_tag="PK513_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOO82710.1"
                     /translation="MPIAPHRRHNKPIAIWLYSCAFMVFAMIVIGAITRLSESGLSMV
                     EWRPLMGALPPLNDAEWERVFTLYQESPEFEKKNTWMQIDDFKTIFFWEWFHRLWGRL
                     IGIVFALPLIFFWLCKMIPHGYHLKLLGLFVLGGAQGFMGWYMVKSGLVDQPAVSHYR
                     LAAHLGLAFIIFASLLWVGLSLTFKTRRYANRALNIHGWIVLVFIAITVLWGAFTAGL
                     DAGLIYNETFPKMGGEWIPPDFWKYDALLPNVLENHSGVQFIHRWLAILTVGMVLSLW
                     MHAMAKKQSFRALHAAVIMALLQLCLGIATILSGVELHIATTHQAGAVILWALILICI
                     YQTWPHKRVC"
     gene            complement(2501..>4929)
                     /locus_tag="PK513_09425"
     CDS             complement(2501..>4929)
                     /locus_tag="PK513_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82711.1"
                     /translation="NVNDMSMDTWNGRYGPPTQDQRMYLYVRNNWHHMKEIFKGDTSF
                     FFNKSGNRFNHGLTYEEAVAQLSPEDRATLDAGMKALDRVSAYTYLVERRPSEKYEDR
                     SYYFELRQDRYVPNVLAADLKNKFGEKADPVAKQEQSGPKKDEDSPQQSLTNKEKEEI
                     PGRPDSNLLEDDGLPPANAAKLATWATSGYGAAKAFNKAGQIAQVVPEASAKHAAAAM
                     KILRIADNAEVGSRKQIDALNRASGLMKEVGLTEAQLRLQGALNGTHPIPESKLDKAG
                     RALKLLVAADNAPQYSPDIARAHELAQKLMDKAGLSETQVRLLGLQNGTEILPEGKEK
                     TANRVLRALEIADNAQTAAKRTQAMESAQRMMKAQGLTEAQVRAFGLAQQNPKAVKEL
                     TKINLDIETKTKQPTKANPAAAADTNTTTKAKQLESTAAAKPSSDTPDKKDHIIKRIE
                     NIIESAKHPSISEKERASRLKSAERLMKKHGITEDQIKEQTPSKTTETTSRKTAQKDA
                     YKITSSEGKALLSERETSKALTLTTKLDEIPKIEAPNILKRATQGLSKLFKRAGKGGA
                     AISKSVFGGTLAGYAAGAGVGITIAAAETSHLESKHDFLIASNLLTKDQHTALANLYG
                     WYIGSMIADPSTVTSEYLIKDAIKDLGLNPVVTEMVSPDMIIDVLEKGLTDAEVKAIK
                     EQFTDKDKLAEMYTSIAKAHGLPEKVTVEINGKPQEISIGVAMLDPKAAKEIWTKYTK
                     LIPDGNDNSDNPEHITRYVADESVNPTERRGLLYLETVNEMTATTTQMVQEYEKTQEM
                     LHTPSNIIAP"
BASE COUNT         1110 a         1170 c         1145 g         1504 t
ORIGIN      
        1 gaagcgctgg ctggccgata attcataatc gtcagattaa gttatgactt ttacagttta
       61 gatgtaatca atcatagcat gccaagcgca ccttcaaccc atccagactt tcatccatga
      121 taatctgaca cgataggcgt gagttatctt gcacgtcaaa tgcttcatca agcatctctt
      181 cttcgtcatc acgcatcatc gccagcttgc ttaaccagtt ctcatcaaca taaacatgac
      241 acgtcgcaca cgcacaagcc ccgccgcatt cggccttaat cggcaatcca tgatcacgaa
      301 taatttccat cacgcgccac ccttcaaccg catccagcgc atgctcaacc ccgttttgat
      361 ctgtcacata aattttcatt tcacactctc accaaatatc ggttttctta ttctcattgt
      421 taaaaactga atacacgcca taagcaacag caagactaga aaccagcgaa agcacaaaat
      481 ctataaaagc ctgaataaca gccatgccgg tttcaaatac gatagaacta tcaccaacac
      541 ccaaaacccc cataaccgtg ccataaaatt cactgagtaa aatcataaac aacatcattg
      601 gcacgaaaca caaaacccaa acacccagca aaggaaacga atccgaatat gcccggaaac
      661 gaatcaaata ggtgcgtaca gatttcccca ttacaaccgg aatataaatc cacaaaaagc
      721 gaaacgccca gatcaaaaat acaataagca tcatcgccaa tataaatgtc tgcaaacttg
      781 gatcgggtgg gggagcgtct ggaatggtct gttgaccgtg ataagccgag ccaacaacaa
      841 aggacagcat cagtttgatc aatacataca caatcatgga agctttaata ttccgcgcag
      901 tatcctctgc aggcggcaaa atagagctgc gcgtttcacg ccgggtttca tgcgcatgca
      961 gcgccataat caccagcgtc gcaatgaccc agccttccat aaaaaaaatc ggtaaaagca
     1021 agagcccctg ccgtaaggcc tctttctgga tatcaaaagc aacaaaacca acaaaaaaga
     1081 gaatcttaag aactaaaacc attgccgaaa agcgcaccac atccacccgt ctgttccaaa
     1141 caaagcgata cgcctgcgat gtactttcta caaaatcaaa actagccacg acgatttttc
     1201 tctttatatt cacctgtata aaccaagatc atacacaatt gtgctttttg taaaagcgct
     1261 gaaatacagg aaaagggttt ttaaaatgcc tatcgcaccc caccgccgac acaataaacc
     1321 tatcgccatc tggctttatt cctgcgcctt catggtcttt gccatgatag tcataggcgc
     1381 aattacgcgc cttagtgaat ccggcctctc aatggttgaa tggcggcctt tgatgggcgc
     1441 tctgcccccc ttgaatgacg ccgaatggga acgcgttttt acgctttacc aggaaagccc
     1501 tgaattcgaa aagaaaaata cctggatgca aattgacgac ttcaaaacca tctttttctg
     1561 ggagtggttt caccgccttt ggggccgtct tattggaatc gtttttgccc tgccactcat
     1621 tttcttttgg ctctgtaaaa tgatccccca cggctatcac cttaaacttc ttggactatt
     1681 tgtactgggc ggcgcccaag gttttatggg ctggtatatg gtcaaaagcg gtcttgtcga
     1741 tcagccagca gttagccact atcgcctcgc cgcacatcta gggctggcat ttatcatctt
     1801 cgccagcctg ctttgggtcg gattatcact cacattcaaa acccgccgct acgcaaaccg
     1861 tgcccttaat atccatggtt ggatcgtttt ggtctttatc gccatcactg tcctctgggg
     1921 agctttcaca gcggggctgg atgcagggtt aatatataac gaaacctttc cgaaaatggg
     1981 cggcgagtgg attcctccgg atttttggaa atatgatgcg cttctaccca acgtccttga
     2041 aaatcatagt ggcgtgcaat tcattcaccg ctggctggcc atccttactg ttggtatggt
     2101 tctaagcctc tggatgcacg cgatggcaaa aaaacaaagc tttcgtgcgc tccatgccgc
     2161 cgtaataatg gcactgctgc aattatgttt aggaatagcc acaattttat caggcgttga
     2221 gctgcacatc gcaaccacgc atcaggccgg tgccgttatc ttatgggcac tgattttgat
     2281 ctgtatctac cagacatggc ctcataagcg cgtctgttag gctcaggact cattaaattt
     2341 gccatatgac gatcgtagcg agctgctatg cggggcgcca gtctttgagt ttggcgaagc
     2401 tgttttcaat gatatgccgt tttttataga gttggtgttc aggccgcctt ttgttttgtt
     2461 gatagcacgt ggaacatttc cttagctgta aaaggcgcca ttagggggca atgatgttgg
     2521 atggtgtgtg cagcatttcc tgcgtttttt cgtattcctg aaccatctgt gtggtggttg
     2581 cggtcatttc gttgaccgtt tcgagatata gcaggccacg gcgttctgtt gggtttacgc
     2641 tctcatctgc aacatatcgt gtgatgtgtt cagggttgtc gctgttgtcg tttccgtctg
     2701 gaattaattt tgtatatttt gtccatattt cttttgcggc tttagggtca agcatggcca
     2761 cgccgattga aatctcttga ggtttgccgt tgatttccac tgtgactttt tcaggtaagc
     2821 cgtgcgcttt tgcaatgctt gtatacattt ctgctagttt atctttatcg gtgaattgtt
     2881 cctttatggc cttcacttcg gcgtcagtta gtcctttttc caagacgtct atgatcatgt
     2941 cggggctgac catttcggtg acgaccgggt tgaggccgag atctttgatg gcgtctttga
     3001 tgaggtattc actggtaacg gtcgagggat cagcgatcat agagccgata taccagccgt
     3061 aaaggttggc tagcgctgtg tgttggtctt ttgtcagaag attggatgct ataagaaagt
     3121 cgtgcttgct ttccagatgg ctggtttctg ctgcggctat ggttatgccg acgcctgcgc
     3181 cagcggcata accagccaag gtcccgccaa aaacagattt tgaaattgcc gcgccgcctt
     3241 taccggcgcg cttgaagagt tttgaaagac cttgagttgc tcttttcaag atattggggg
     3301 cttcaatttt cggaatttca tcaagttttg ttgttaacgt aagtgcttta gatgtttctc
     3361 tttcagaaag gagtgctttt ccttcagaag aggtaatctt gtaggcatct ttttgtgctg
     3421 tttttcttga agtcgtttcg gtggttttcg aaggtgtctg ttctttgatt tgatcttctg
     3481 ttatcccgtg ttttttcatt aaacgttctg cactttttaa tctgcttgca cgttcttttt
     3541 cacttatgct tggatgcttt gcgctttcaa taatgttttc aattctttta attatatgat
     3601 cttttttgtc aggagtgtcg cttgatggtt ttgcagcagc tgtgctctca agctgtttag
     3661 cctttgttgt cgtattcgta tccgcagccg cagccgggtt tgcttttgtc ggctgttttg
     3721 tctttgtttc tatgtctagg tttatttttg ttagctcttt aacagctttc ggattttgct
     3781 gcgcaaggcc gaaggcgcgg acctgtgctt cggtcaagcc ttgggctttc atcattcttt
     3841 gagctgattc catggcttgc gttcgtttgg ccgcagtttg cgcgttgtcg gcaatttcca
     3901 gggcgcgtag aactctgttt gcagtttttt ctttgccttc aggaaggatt tcggtgccgt
     3961 tttggaggcc gaggagacgg acctgcgttt cgctgaggcc ggctttgtcc atgagttttt
     4021 gtgcgagctc atgagctctt gcgatgtccg gagagtattg cggtgcattg tcggcggcga
     4081 ccaataattt aagtgcgcga cctgctttgt ccagcttgct ttccgggatg ggatgggtgc
     4141 cgttaagggc gccttgcagg cggagctgcg cttcggtgag gcctacttct ttcatgagcc
     4201 cgcttgcgcg gtttaaagcg tcaatttgtt ttcttgagcc aacttctgcg ttatcggcaa
     4261 ttcttagaat tttcattgct gctgctgcgt gtttggctga agcctcagga acaacttgtg
     4321 ctatttgtcc ggctttgttg aaggcttttg ctgcgccata gcctgacgtt gcccaagttg
     4381 ctaattttgc tgcatttgcc ggcggaaggc cgtcatcttc cagtaaattg gaatctggtc
     4441 tgccgggaat ttcttctttt tctttgttgg taagactttg ctgcgggctg tcttcgtcct
     4501 ttttggggcc gctttgctct tgtttggcta ccggatcggc tttttctcca aatttatttt
     4561 taaggtctgc ggctaacacg tttggcacat atcggtcctg acgcagttcg aagtaatagc
     4621 ttctgtcttc gtatttctca ctggggcggc gttcgaccag ataggtgtag gctgagacgc
     4681 ggtccagcgc tttcatgcct gcatcaaggg ttgcgcgatc ttccgggctg agttgtgcaa
     4741 ccgcttcttc gtaggtcagg ccgtgattaa agcgattgcc gcttttatta aagaaaaagc
     4801 ttgtgtcgcc tttaaagatt tctttcatgt ggtgccagtt attacgcacg taaagataca
     4861 tgcgctggtc ttgcgttggc gggccgtagc ggccgttcca ggtgtccatg ctcatgtcgt
     4921 tgacgttgc
//
LOCUS       DAOSWR010000149         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11726_length_4868_cov_5.675047, whole genome shotgun sequence.
ACCESSION   DAOSWR010000149 DAOSWR010000000
VERSION     DAOSWR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11726_length_4868_cov_5.675047"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK513_09430"
     CDS             <1..341
                     /locus_tag="PK513_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOO82712.1"
                     /translation="AEVHDASAFGEVVQAENLGFCPRGEGGIFAEKGETSLKGRIPIN
                     PSGGLISRGHPIGASGLAQVHELVTQLRGRAGKRQVKNARLALAENGGGALGTEEAAM
                     CIHILEAPSR"
     gene            472..1980
                     /locus_tag="PK513_09435"
     CDS             472..1980
                     /locus_tag="PK513_09435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO82713.1"
                     /translation="MITPKENKSLLEREPRFEDSFISLQAIPKIPKLQLRETRALISH
                     ARRFNDEVARPMALKTELKTFQDPDYIPWELVNKANEWGFYTMFIPKLFGGQGYAMPA
                     NCYVIEELSSACVGLANVAFVHYLGFMTVLASWNIPLINRVCRDVAEGERTGNPCLIS
                     LAITEPGAGTDTEETQLLDRGKITCYAEKVKGGYVVNGSKIFISNGHVSTWCALVAYT
                     DLKKPSANTVTFAVKTGTKGFTFGKHEDKMGQRACVASELIFEDCFIPDDCVMTDSRY
                     LPNSQIREAADLVRQYIHYVVEVTRASVGAFGTGVARGAYEQALKFAAETEVEGKLLI
                     NHEWAQIMLAEMYKNVVTGRLTYTEANYAHSLPGGVFHFLQLKPVYYYLKFIPQFVID
                     MFFVPFYKMKIVDRLIRKIFVDGQSMDAQERSSGYASIAKFVGTDMGVKNSQMALEMM
                     GQAGLKHEVGAEKILRDSKLLQIYEGTNQLNRLNLFDCLIARCVPQAKVFEE"
     gene            1993..3114
                     /locus_tag="PK513_09440"
     CDS             1993..3114
                     /locus_tag="PK513_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO82714.1"
                     /translation="MLTTIKCELKSFEDLAHSFAAKELTANREERDRYPFGPFFDSVI
                     DKAYEVGILGATLPEECGGIGQDVSALCIILDHISAADASLAGIIFTNALSQEIILAA
                     GNKEELSSITSQASNANAALIACPSFANPSETGVALEAVKIKDTYMLNGAIEYVVLGG
                     LSGHALVPAKIRGQEGYSYFLINLNDKGIYKNQPVFSLGLHACPAVDINVAGVEAKLI
                     GKEGAGAHYFNQASDRMHSAVAAIQCGIMKGSFNEALDYSQQRCQGGKEIIHWSGLRM
                     ILGQMAVQVKIAEMVVSEAAMAVENREPQWNLYARAAALHLSELACTLTTDGIQCLGG
                     NGYMKDYGQEKRFRDAKHTQALLGLSPMRKLSMIRDMLK"
     gene            3377..>4868
                     /locus_tag="PK513_09445"
     CDS             3377..>4868
                     /locus_tag="PK513_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO82715.1"
                     /translation="MYVLANLRIAGSIAKSLLTFFKDEMGRGTLGANLKSMIKAQEIT
                     EDMSWAELIEEKARKYGDRTFLLFEDKEFSFRQMDENANRVANYLLTLGAGKSKGVAI
                     IMGNSPQYLDVYIGSQKIGMYSIPINTSLRGDSLLYILDHSDAEFVVIDEEFLDAYKK
                     IADKVEKRKTVIVNRSTPAGESALPQGMLNLSDAYGQKPNKPNIKYDKNDICFILYTS
                     GTTGLPKGVMYRYGKTTVKLLSIPANALYKKSDILYTSLPLFHGNALWLCVTQAMHRG
                     CKVVLARKFSASKFWDEMRKYKVTEFNTIGAMIPILMKQPPKENDRDNCVRFTISAAC
                     PVDEWEKFEKRFGIKIYEAYGSVDGGGKSIMNMGNAPVGSIGKPAPNTVYRLVDNEGN
                     DVPDGTPGQLIFESKGKKKSVEYFKNEKAGNDKLKGGWIYTGDLVRRDKNGYLYFVGR
                     NAEFMRIKGENVSAYEVEHTIQKHPSVLEAAVYAVPSELAEDEIMAC"
BASE COUNT         1317 a         1166 c         1262 g         1123 t
ORIGIN      
        1 tagccgaagt acacgatgct tcggccttcg gtgaagtggt gcaggcggaa aatctaggct
       61 tttgtcccag aggcgaaggt ggaatttttg ccgaaaaagg agaaacgagc ctgaagggcc
      121 gcattcccat caatcccagc ggcggtttga tttcgcgcgg ccatcccatc ggtgcatccg
      181 gtctggccca ggtccatgaa ctggttaccc agttaagagg cagagccgga aaacgtcagg
      241 tcaaaaacgc cagactggcc ctggcggaaa acggcggagg cgctctgggt acggaagaag
      301 cggcgatgtg catccatatt ctggaagcgc cgtcacgtta aattgacaat aagtattggc
      361 aggctgttgc gagatccact tttgacgagc atagagtacc gcacttcttg aaattcgaag
      421 aacaaaggta ttccaaattg ttcagacata attaatgcgg aagggggtat tatgatcacg
      481 ccgaaggaga acaaatcttt attggaacga gagccgcgtt ttgaggacag ctttatcagt
      541 cttcaggcca ttccgaagat tcccaagttg cagctcaggg aaacgagagc cttgattagc
      601 cacgcgcgca gattcaacga cgaagtcgcc aggcccatgg ctctcaaaac ggaattaaag
      661 acttttcagg atccggatta catcccgtgg gaactcgtca ataaggccaa tgaatggggt
      721 ttctacacca tgtttatccc caaattattc ggcggccagg gatatgccat gccggcgaac
      781 tgttatgtca ttgaggaact atcttccgcc tgtgtgggtc ttgccaatgt tgcttttgtt
      841 cactatctcg gttttatgac cgtactggca tcatggaata tcccgttgat caacagagtc
      901 tgccgtgacg tggccgaagg ggaacgcacc ggcaatccct gtcttatttc tctggcgatc
      961 accgaaccgg gcgccggcac cgatacggaa gaaacgcaac tgctggaccg cggcaagata
     1021 acctgctacg ccgaaaaggt caaaggcggt tatgtggtca acggtagcaa aatatttatt
     1081 tccaacggtc atgtgtccac ctggtgcgct ctggtggcct acacggattt gaaaaaacct
     1141 tccgccaata cggtaacctt tgcggtgaag accggcacga aaggattcac gttcggcaag
     1201 catgaagata aaatgggtca aagggcatgc gtggcgagcg aactgatttt tgaagactgc
     1261 tttataccgg atgactgcgt tatgactgat tcgcgttatt tgcctaactc ccagatccgc
     1321 gaggccgccg atctggtgcg tcagtatatc cattatgtgg tggaagtcac acgcgcctcg
     1381 gtgggggctt tcggaaccgg cgtcgcccga ggggcttatg aacaggccct caaatttgcc
     1441 gctgaaaccg aggtggaagg caaactgttg atcaatcacg agtgggcgca gatcatgctg
     1501 gccgagatgt ataagaatgt tgttacagga cgtctcacct atacagaagc caattatgcg
     1561 catagcctgc ccggcggcgt ctttcatttc cttcaactca aaccggttta ctattatctt
     1621 aaattcattc ctcagtttgt catcgatatg ttctttgttc ctttctataa aatgaaaatc
     1681 gtggatcggt tgatcaggaa gatttttgta gacgggcaat ccatggacgc gcaggaacgt
     1741 tcttccggat acgcatccat tgccaaattt gtcggcacgg atatgggcgt gaaaaacagt
     1801 cagatggcgc tggaaatgat ggggcaggcc ggtttaaaac acgaggttgg ggcggaaaaa
     1861 attctgcgtg attccaaact gctgcaaatt tatgaaggca ccaatcaatt aaaccgtctt
     1921 aatcttttcg attgcctgat tgcgcgttgt gttccccagg ccaaggtatt cgaggaatag
     1981 gaggaaatat aaatgctgac aaccataaaa tgcgaactca agtcgtttga agatctggcg
     2041 cacagctttg cggcaaaaga gcttaccgcc aaccgggaag agcgcgaccg ctatcctttc
     2101 ggtccttttt tcgactccgt tatagacaaa gcctatgaag tcggaatttt aggcgccacg
     2161 ctgccggagg aatgcggcgg cattggtcag gacgtgagcg ccttatgcat tatcctcgac
     2221 cacatcagcg cggcggatgc cagccttgcc ggaatcatat tcaccaacgc tttatcccag
     2281 gaaatcatcc tggccgcggg caacaaagaa gagctctctt ccatcacgag tcaggcgtcc
     2341 aacgctaatg ccgctctgat cgcctgcccg tcctttgcca atccctccga aaccggcgtt
     2401 gcactggaag ctgtcaaaat taaagacact tacatgctga acggcgccat cgaatacgtc
     2461 gttctgggcg gcctttccgg tcacgcgctg gttccggcta aaatcagggg acaggaaggc
     2521 tacagttact ttttgatcaa cctgaatgac aagggcatct acaaaaatca gccggtattc
     2581 agtctgggac tccatgcctg cccggccgtg gatatcaatg ttgcaggtgt agaagcaaag
     2641 ctgatcggta aagaaggagc aggagcccat tacttcaatc aggcttccga ccggatgcac
     2701 tcggccgttg ccgccatcca gtgcggcatc atgaaaggct cttttaacga agccctcgat
     2761 tacagccagc agcgctgtca gggtggaaaa gaaattatcc actggtcggg actgagaatg
     2821 atcttaggcc agatggccgt tcaggtgaag attgccgaga tggtcgtctc cgaagcggcc
     2881 atggctgtgg aaaacagaga accgcagtgg aacctctacg ccagagcggc ggcgcttcat
     2941 ttgtcggagc tggcctgcac actgacgacg gacggcatcc agtgtttagg cggaaacggc
     3001 tatatgaagg attacggcca ggagaaacgg tttcgcgacg cgaaacacac gcaggcactg
     3061 ttgggactgt ctcccatgcg caagttgtcc atgatcaggg acatgttgaa atagaaaaat
     3121 cacgggtatg aaccccaaag caagctttgg aaactattcc gcagcaagct gcggggtatt
     3181 aaaccctccg ctgcgcggga ttgttcgact tacaaatttc aatgcactac gtttttgaaa
     3241 tttgagtctc acaataaaac gttcctaccc ccccccaagt cggtaggtaa ccgaaggaac
     3301 cctgatagcg tcttcaggcg atatcagggt tcctgactac tcagaaattt attaatttat
     3361 ttagagaggt acgaaaatgt atgtgttagc gaatttaaga attgcaggtt cgattgcaaa
     3421 aagtttatta acatttttta aagatgagat ggggcgtgga acgctcgggg caaatttaaa
     3481 gagtatgatt aaagcgcagg aaatcacgga agatatgtcc tgggctgagc tcatcgaaga
     3541 aaaagcccgc aagtatgggg acagaacatt tctgcttttc gaagacaagg aattttcctt
     3601 tcggcagatg gacgaaaacg ccaacagggt agccaattat ctcctaacgc tgggcgccgg
     3661 caagagcaag ggcgttgcca tcatcatggg caacagcccc cagtatctgg acgtctatat
     3721 cggttcgcag aaaatcggga tgtattccat tcccatcaat acatcgttgc gcggcgacag
     3781 tcttctctat attctggacc acagcgatgc ggaatttgtc gttattgacg aggaattcct
     3841 cgatgcctac aaaaaaatag cggacaaggt agaaaaaaga aaaacagtaa ttgtgaaccg
     3901 atctacccct gcaggcgaat ctgcccttcc tcaagggatg ttgaatcttt ccgacgctta
     3961 tggtcaaaaa ccgaacaagc caaatataaa atatgataaa aacgatattt gcttcatcct
     4021 ttacacttcg ggaacaaccg gcctgcccaa gggggtaatg taccgctacg ggaaaaccac
     4081 ggtgaagtta ctttcgatac cggcaaatgc actgtacaaa aaatcggata ttctctacac
     4141 cagtctccct cttttccacg gcaacgctct ctggctctgc gtcactcagg ccatgcaccg
     4201 tggctgcaag gtggtactgg ccagaaaatt ctccgcgagt aaattctggg acgaaatgcg
     4261 caagtacaag gttacggaat tcaataccat cggcgccatg attcctatct tgatgaaaca
     4321 gccgccgaag gaaaatgatc gtgacaactg cgtacgcttt actatatcgg ccgcatgccc
     4381 ggtggacgaa tgggagaaat tcgaaaagag attcggcatt aaaatctatg aagcttacgg
     4441 ctccgttgac ggtggaggca agagcatcat gaatatggga aacgctccgg tcggttctat
     4501 cggcaaaccg gcacccaaca cagtatatcg gctggtggat aacgaaggca acgatgttcc
     4561 ggacggcact ccgggtcaac tcatttttga atccaaaggc aagaaaaaat cagtggaata
     4621 ttttaaaaat gagaaagccg gcaatgataa gctgaaaggc ggctggatat acaccggaga
     4681 cctggtcaga cgggataaga acggttatct ttactttgtc ggacgcaatg cggaattcat
     4741 gcgcatcaag ggcgaaaacg tttccgccta tgaagtggaa catacgattc aaaaacaccc
     4801 ctcggtgctc gaagctgctg tttatgccgt cccctcagaa ttggccgaag atgaaatcat
     4861 ggcctgtg
//
LOCUS       DAOSWR010000150         4862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11745_length_4862_cov_4.948409, whole genome shotgun sequence.
ACCESSION   DAOSWR010000150 DAOSWR010000000
VERSION     DAOSWR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11745_length_4862_cov_4.948409"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /gene="mutS"
                     /locus_tag="PK513_09450"
     CDS             <1..1415
                     /gene="mutS"
                     /locus_tag="PK513_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO82716.1"
                     /translation="TAELQDKLTQALETEPPMLARDGGFIRTGFFKKLDDLKTLKSES
                     KRLIAGLQSQYQKDTGIDALKIKFNNVLGYFIEVPARRADPLMEKGPESPYIHRQTMA
                     SAVRFTTTALAELERDLSSAGEKALAIELEIFQQLTEECVAASEEIGQRARAIATLDM
                     ATALAQLASDQNYCRPLLDNSLAFTLESARHPVVETALKKDHETFVPNDCTLTPDNHL
                     WLLTGPNMAGKSTYLRQNALIAIMAQTGSFVPAKKAHIGIIDRVFSRVGASDDLARGR
                     STFMVEMVETAAILNQATDRSLVILDEIGRGTATFDGLSIAWACVEHLHDVNKCRALF
                     ATHYHELTALTSRLPALSCHSMQVKEWQGDIVFLHSVAPGAADRSYGIHVAKLAGLPE
                     SVIARARSILEKLQTGEQSGNLARLSEDLPLFSLADNKQSAAHINPALEKLETITPDT
                     LSPREALDVLYALKTLLKDS"
     gene            1555..2634
                     /locus_tag="PK513_09455"
     CDS             1555..2634
                     /locus_tag="PK513_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO82717.1"
                     /translation="MTNAKTTAPQKDTITTDAIIIGAGPCGLFCAFELGLLDMKCHFV
                     DILDRPGGQCTELYPEKPIYDIPGYPEITGQELTDKLMKQIEPFHPTFHLNQMAEKLE
                     KLDNGNWQLTTDIGTILEAPVVIIAAGGGSFVPKKPPIPGIEEYEGASVFYAVRKMEQ
                     FKDKKLVIVGGGDSALDWTLNLQPLAKQLTLIHRRPEFRAHMDSVNKMRSLENEGKIN
                     FQLGQVKELKGENGQLSSVIIEHKDKTTTKIECDCLLPFFGLTMKLGPIADFGLNLHE
                     NLIPVDTEKFETSTPGIFAVGDINHYPGKLKLILSGFHEAALMSRQAFRYIYPDAKLK
                     FEYTTSSSSLQEKLDVPTAKEKKTA"
     gene            complement(2670..3686)
                     /gene="cysK"
                     /locus_tag="PK513_09460"
     CDS             complement(2670..3686)
                     /gene="cysK"
                     /locus_tag="PK513_09460"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOO82718.1"
                     /translation="MNSPADKSAKPALDSRPLRPDGESRGKIYNNLLETIGNTPLVRV
                     PKFVGKYGLEADILCKLEFFNPVSSVKDRIGFAMIEAAERAGKITPGKSVLVEPTSGN
                     TGIALAMVAASKGYRLILTMPESMSLERRKMLRFLGAELVLTPAEKGMKGAIARAEEI
                     VSEKGEAFMLSQFDNPANPQIHRETTAMEIWNDTDGAVDILVAGVGTGGTVSGVSKVL
                     KEKKPGFLAYAVEPAESPVISGGEPGPHKIQGIGAGFVPDNLDTTLIDGVVKISSEEA
                     IETAREVARLEGIPCGISSGAAMAAAKQIAKMPESKGKQIVVMIPSIAERYISTALFE
                     DIED"
     gene            complement(3752..4090)
                     /locus_tag="PK513_09465"
     CDS             complement(3752..4090)
                     /locus_tag="PK513_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOO82719.1"
                     /translation="MTFVVTDVCIKCKYTDCVEVCPVDCFYEGENMLVISPDECIDCG
                     VCEPECPIDAILPDSDSKAEKWVEMNREYSEKWPNITSTRAPMPDAEKMKDVPNKMEE
                     HFSPKPGEGD"
     gene            4360..4809
                     /locus_tag="PK513_09470"
     CDS             4360..4809
                     /locus_tag="PK513_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82720.1"
                     /translation="MTHQIIDPDDAILRESVSYFLENSEAPSASTYDFERIDLNEDGR
                     RDALILFKTPYGYWCGTHGCTMLILRAHNNHFSLVNDIQPIREPVYISPAKSHGWKNL
                     LVRVSGRWDKAKNVVMAYNGEKYPNDPSNLPPFDQYNDDEYVRALYN"
BASE COUNT         1331 a         1587 c          924 g         1020 t
ORIGIN      
        1 ttaccgccga attacaagac aagctcaccc aggccctcga aacagaaccg ccaatgcttg
       61 cgcgcgatgg cggcttcatc cgcacaggct ttttcaaaaa gctcgacgac ctcaaaacgc
      121 taaaaagcga gagcaaacgc ctcatcgccg gcctgcaaag ccaatatcaa aaagacaccg
      181 gcatcgacgc gctcaaaatc aaattcaaca acgtgctggg ctattttatc gaagtccctg
      241 cccggcgcgc cgatccgctg atggaaaaag gccccgaaag cccatacatt caccgccaga
      301 caatggcgag cgccgtgcgc ttcaccacca ccgctctcgc cgaacttgaa cgcgacctct
      361 cttccgctgg tgaaaaagcc ctcgccatcg aactcgaaat cttccagcaa ctcaccgaag
      421 aatgcgttgc ggcttccgaa gaaattggcc aaagagctcg cgccatcgcc accctcgata
      481 tggctaccgc actcgcccaa ctcgccagtg accaaaatta ttgtcgccct cttcttgata
      541 acagcctcgc ctttaccctc gaatccgccc gccacccggt cgtcgaaacc gcgctcaaaa
      601 aagaccacga aaccttcgtg cctaatgatt gcacccttac ccccgacaat cacctctggc
      661 tgctcaccgg ccccaacatg gcaggaaaat ccacctattt gcgccaaaat gccctcatcg
      721 ccattatggc gcaaaccggg tcattcgttc cggcaaaaaa ggcgcatatt gggatcatcg
      781 atagggtttt cagccgtgtc ggagcatcag atgacctggc gcgtggacga tccaccttca
      841 tggtcgaaat ggtcgaaacc gccgccatcc tcaaccaggc caccgaccgc tccctcgtta
      901 tccttgatga aatcggccgc ggcaccgcca cctttgatgg cctgtccatc gcctgggcct
      961 gcgtcgaaca cttgcacgat gtcaataaat gccgcgccct tttcgccacc cattaccacg
     1021 aacttaccgc cctgacatca cgcctgcccg ccctgtcctg ccactccatg caggtcaagg
     1081 aatggcaagg agacatcgtt ttcctccaca gcgttgcccc cggcgctgcc gatcgttcct
     1141 acggcatcca cgttgccaag ctcgccggcc tgccagaatc cgtcatcgcc cgcgcccgga
     1201 gcattctgga aaaactccaa accggtgagc agtccggtaa tctcgcccgg ctttccgaag
     1261 acctgccgct attctccctc gccgataaca agcaaagcgc cgcgcacatc aaccccgcac
     1321 tcgaaaagct cgaaaccata acacccgata cgctctcccc acgcgaagcc ctcgacgtgc
     1381 tctacgccct caaaacactc ctcaaagact cataacctat acttgaccac cctaaagccc
     1441 cccctttaag caaagccacc ttgcattcac ctgtataaaa tattatatcg acaacagaat
     1501 aaaaacagca gaaaaaacaa aaacacagca caagacaaca caaggataaa ggctttgaca
     1561 aacgccaaaa ccacggcccc gcaaaaagac acaataacca ccgatgccat tatcataggt
     1621 gcaggcccct gcggcctgtt ctgcgctttc gaacttggcc tgctggatat gaaatgccat
     1681 ttcgtcgaca tcctcgaccg ccccggcggc caatgcacag agctttaccc cgaaaaacca
     1741 atttacgaca tccccggcta cccggaaatc accggccagg aacttactga taagttgatg
     1801 aaacagatag aacccttcca tccgacattc caccttaacc aaatggctga aaaactggaa
     1861 aagcttgata acggcaactg gcaactcacc accgacatcg gcacaatcct cgaagccccc
     1921 gtcgtcatca tcgctgccgg cggcggcagc tttgtcccca aaaagccccc catccccggc
     1981 atcgaagaat acgaaggtgc atcagtcttc tatgccgtgc gcaaaatgga acaatttaaa
     2041 gacaaaaaac tcgtcatcgt tggcggtggt gattctgcac tggactggac cctgaacctt
     2101 caaccactgg ccaaacaact caccctcatt catcgccgcc ctgaattccg cgcacatatg
     2161 gacagcgtca ataaaatgcg cagcctcgaa aacgaaggga aaatcaattt tcagctcggt
     2221 caggtcaaag agctcaaagg cgagaacggc cagctttcct cagtaatcat cgaacataaa
     2281 gacaaaacaa ctacaaaaat cgaatgtgat tgcctgctgc ccttcttcgg cctgaccatg
     2341 aaactcggcc ctatcgcaga tttcggcctg aatttacacg aaaacctcat ccccgtggat
     2401 acggaaaaat tcgaaacatc aacgccgggc atcttcgccg tcggcgacat taaccattac
     2461 cccggcaaac tcaaactcat cctctccggc ttccatgaag ccgctctcat gagccgccag
     2521 gccttccgct atatctaccc cgatgccaaa ctgaaattcg aatacacaac ctccagctca
     2581 agccttcagg aaaaactcga tgtcccgacc gccaaagaaa agaaaactgc ttaggatcaa
     2641 aaaatctaca taacgtagat aataaccgct taatcctcaa tatcctcaaa aagagccgta
     2701 gaaatatacc tctccgcaat tgacggaatc atcaccacaa tctgcttgcc cttgctttca
     2761 ggcattttcg caatctgctt ggccgctgcc atcgccgcgc ccgatgaaat accgcaagga
     2821 atgccctcca gccgcgccac ctcgcgcgcc gtttcaatag cttcttcact gctgattttg
     2881 actacaccgt caatcaacgt cgtatccaga ttatccggca caaaccccgc gccgatcccc
     2941 tgaatcttat gcggccccgg ctcaccaccg gaaatcaccg gactttctgc cggctccacc
     3001 gcataggcca aaaaccccgg ctttttctct ttaagcacct tggaaacgcc gctcacagtg
     3061 cccccggtcc cgacaccggc aacaagaata tccacagcac catccgtatc attccatatc
     3121 tccattgcgg ttgtttcacg gtgaatttgc ggattggccg gattatcaaa ctgactaagc
     3181 ataaacgctt cgcccttctc agaaacaatc tcctcagccc gcgcaatcgc gcccttcatt
     3241 cctttttccg ccggtgtcaa caccagctcc gcccccaaaa agcgcagcat cttgcgccgc
     3301 tccaaagaca tgctttccgg catcgtcaaa atcaaccgat acccctttga tgccgccacc
     3361 atcgccagcg caatacccgt atttccagac gtcggctcca ccaaaacact cttgcccggc
     3421 gtaatctttc ctgcgcgttc agcagcctca atcatcgcaa aaccaatccg gtctttcacc
     3481 gaagacaccg gattaaaaaa ttccagcttg cataaaatat cggcctcaag cccatatttt
     3541 ccaacaaatt taggcactcg cacgagcggc gtattcccga ttgtttccaa aaggttattg
     3601 taaatcttcc cgcggctctc tccatcaggc ctcaacggcc ttgaatctag cgcgggcttc
     3661 gcagatttat cagctgggga attcatactc taatctcctt actcaaatta gctcctcaaa
     3721 cacacaaaca tttcagacga aaagacaaag attaatcacc ctcgccaggt ttcggactaa
     3781 aatgctcttc catcttattc ggaacatctt tcatcttttc cgcatccggc atcggcgccc
     3841 gcgtgctggt aatattgggc catttttcag aatattcccg gttcatctcc acccactttt
     3901 ccgccttgct atctgaatca ggcaaaatcg catcaatcgg gcactccggc tcacacacac
     3961 cgcaatcaat gcactcatcc gggctaataa ccagcatatt ctcaccctcg taaaaacaat
     4021 caaccgggca aacctcaacg caatcggtat acttgcactt tatacacaca tctgtgacca
     4081 caaaggtcat ggcacattct ccttatatct atgcgcttga ttatcaataa aaccgctcaa
     4141 aaacattaag ccccaaaccc ttataacgca aggggtctga taggaaaatt tatatgtgag
     4201 aaactccata aagccactaa agctcaatag acgcttcaac aaaaccgcga taaccttata
     4261 actatgcgca ccctgtattt tttgacatgc ttttgcctgc ttccgctttt actggggggc
     4321 tgttcgcatc ctcctccacc agacgccgag ctcactccaa tgacacatca aataatagac
     4381 cccgatgacg caatcctcag agaatccgtc agctacttcc tcgaaaatag cgaagccccc
     4441 agcgcatcga cttatgactt cgaacgcata gacctgaacg aagatggccg ccgcgacgct
     4501 ttaatcctgt tcaaaacacc ttacggttac tggtgcggaa cacatggctg caccatgctt
     4561 attctgcgcg ctcataacaa ccatttctca cttgtcaacg acatccagcc cattcgcgaa
     4621 ccggtctata tcagccctgc aaaaagccac ggctggaaaa acttgctcgt acgcgtatct
     4681 ggccgctggg ataaagctaa aaacgttgta atggcatata atggagaaaa atacccgaac
     4741 gacccatcaa atttgccccc attcgaccaa tacaatgatg acgaatatgt ccgcgcactt
     4801 tataattaac tgtgaaactc cacgggccaa ggcccgtggt atcactttag ttcaagctgc
     4861 gc
//
LOCUS       DAOSWR010000151         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11788_length_4847_cov_5.805092, whole genome shotgun sequence.
ACCESSION   DAOSWR010000151 DAOSWR010000000
VERSION     DAOSWR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11788_length_4847_cov_5.805092"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK513_09475"
     CDS             complement(<1..250)
                     /locus_tag="PK513_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO82721.1"
                     /translation="MGDVRHFLDLDDIDPDVLKSILKKAHALKAQKYNPPQIFTGLSL
                     AMVFDKRSTRTRMSFEIAMKQLGGHTLVMSMDEMQITGA"
     gene            complement(337..891)
                     /locus_tag="PK513_09480"
     CDS             complement(337..891)
                     /locus_tag="PK513_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82722.1"
                     /translation="MYKTEGEAIDGHLFLGQSLYYGNEKVFETAVRAIKKGFHTNQRE
                     VIMSGDLSCSFFNLQRGENGISHKLVEGRYMSLRHPQTFHENLVLWADMIVPDLVRNG
                     FFGKETGPDKQRAQIIAGSCHHKDCLFAFLHYGDELSERTLDIAERWLPETDSLRKAI
                     SFAGDSQANLKTSIAGQRLDTLGL"
     gene            complement(965..2167)
                     /locus_tag="PK513_09485"
     CDS             complement(965..2167)
                     /locus_tag="PK513_09485"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HOO82723.1"
                     /translation="MSIDAKKIIDDSFRYLVKTYAPQPVVMDHGKGCYAFDTSGKKYV
                     DCAAGIAVASLGHAHPAMLKTINEQAARMMACQASYTTKEKFKAAKLLVETSCFDLVY
                     FSNSGTESVEAALKCARKWAYDEKGEDCNEIIAFHNSFHGRTYGSASVTEKRNSQPFF
                     APYLPGVHFATFNDIDSVKTLATDKTAAVIIEPIQGEGGLTPANPEFLKALRQFCDEK
                     NICLIFDEVQAGFGRLGALYAYQSFGVEPDIVTWAKGMGSGFPVGAMAAKRKFGDALE
                     VGMHGTTYGGNPLACAVVVTVLEEMLKPGFMEHVNKISGILFDGLNEIKRSSNKITDI
                     RGKGLMIGVDTVFDIKNLMRALQSNGLMTTQAGKETLRLTPPLILSEDEAKEALEIIE
                     KTLSEMGE"
     gene            complement(2183..3082)
                     /locus_tag="PK513_09490"
     CDS             complement(2183..3082)
                     /locus_tag="PK513_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82724.1"
                     /translation="MSKKTKMPEFFINAFYESKFKDNFFVIKAGGKIIEDEGALENLL
                     SNIRDLTLQGIRVLLIYGGGHAMDEEAEKCGVKVNKIGGKRVTNAANMEIARHVLGGD
                     LSLSVSAMMAKIKLDGLSFNCVPPSWMRVVLKPKTEGDDFAGAVEDVYARPINRLFRS
                     VNFVACTCLAQTGDGVTCNINADTIATEIAIGTQAHKLIFLSDVDGVKIGGRTAEIIT
                     AEQIDGYIADGTATGGMQVKLENCKAALDAGVRRIHLLSGLRQDALKKEIYEPVGPGT
                     MLFKDSERQSYENEVQAHKLIAG"
     gene            complement(3091..4083)
                     /gene="argC"
                     /locus_tag="PK513_09495"
     CDS             complement(3091..4083)
                     /gene="argC"
                     /locus_tag="PK513_09495"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOO82725.1"
                     /translation="MIKASIIGITGYTGLEQLRMLLAHPNVEIVHLTSRQHQRVAIGE
                     VYPHLAHLDLTITNTDHMKVAEDSDVVFLALPHKTAQDIVADLHGSVKVIDLSADYRL
                     DDAAVYEKYYTAHNHPELLKEVVYGAPEFNAEKIAVASTVSNPGCFALLAQLMLYPFA
                     GLIERADIMAVTGSSGSGKSASDGTHHPVRNHNMKSYNINKHRHIPEITRSAQIDESQ
                     LNFVPTSGPFSRGIFASAFVHLKADIEPLDLAEQAYNEAPFVRLQDTPALANVVGSNF
                     CDLSFVKGVGQSVIVQGALDNLVKGAAGCAVQNMNLMFGLDETAGLDTLTPLYP"
     gene            complement(4192..>4847)
                     /locus_tag="PK513_09500"
     CDS             complement(4192..>4847)
                     /locus_tag="PK513_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82726.1"
                     /translation="IGQAALYKPVQSMKSNVPLIITTGTSMDSGKTTVAIEIVKTLTR
                     MGMKLAGAKLTGVGAARDLFKMHDYGIYNGVSFVDCGITSTAQMPDAEMVAVAKGAID
                     HLSKSNPDAIMIEFGDGLMGKYGVEAILRDPQIQKNVRFHIGCARDPVGAIGMADFCE
                     QIGLPIDLMSGPVTDNQVGKDILKERKNMMTYNAFSPDNAWLDLVIARWAVEYQDAA"
BASE COUNT         1217 a         1352 c         1011 g         1267 t
ORIGIN      
        1 cggcccccgt gatttgcatt tcatccatgc tcatcaccag cgtatgcccg ccgagctgtt
       61 tcattgcaat ttcaaagctc atccgtgtgc gtgtcgagcg cttatcaaag accatcgcca
      121 gactcagccc cgtaaaaatc tgcggcggat tatatttctg cgctttgagc gcatgcgctt
      181 tctttaggat agatttcagt acatcaggat caatgtcatc caaatccaga aaatgtctaa
      241 cgtcgcccat atttttcctc ttttgtataa ttggcaaaaa ccatctatct cggccatgaa
      301 atacacagat gtttaaaaga gcaagtgctt tttgtcctac aggccaagag tatctagccg
      361 ttgtccagct atagaggtct tcaggttggc ttgagaatca cccgcaaaac tgatggcctt
      421 tcttaagcta tcggtctcag gcaaccatct ttctgctata tcaagagtgc gctcggaaag
      481 ctcatcgcca taatgtagaa aagcaaataa acaatctttg tgatggcacg aacctgctat
      541 gatttgggca cgctgcttgt caggacctgt ttctttacca aaaaagccgt ttcgcacaag
      601 gtcaggaaca atcatatcag cccataaaac aagattttca tgaaaagttt ggggatgccg
      661 caaagacatg tagcggcctt ccacaagctt gtgtgaaatt ccgttttcac ctcgttgaag
      721 gttaaaaaaa ctgcaagaga ggtcgcccga cataatgact tctctctggt ttgtatgaaa
      781 accttttttg atagcgcgaa cggcggtctc gaaaaccttt tcattgccgt aatataaaga
      841 ctgacctaaa aataaatgac catctatagc ttcgccttca gttttataca tagccgcatc
      901 tttcagttgt gttaaaggag ggcaaagtat agaagggcgc aagttatgtc aacaaaaaag
      961 ccagctactc tcccatctcg ctcaacgttt tctcgatgat ttccagcgct tctttcgctt
     1021 cgtcctcgct caggatcaag ggcggggtca ggcgcaaagt ctctttaccc gcttgcgttg
     1081 tcatcagccc gttactctgc aaagctctca tcaggttttt aatatcaaaa accgtatcca
     1141 cgccgatcat cagccccttg ccgcgaatat cggtgatttt attagaagag cgtttaatct
     1201 cattaaggcc atcaaataaa atgccggaga ttttattaac atgttccatg aaaccgggct
     1261 taagcatttc ctcaagcaca gtgaccacca cggcgcacgc cagcgggttc ccgccatagg
     1321 tcgtgccgtg catgccaacc tcaagagcat ccccaaattt tctcttcgca gccatcgcac
     1381 caacaggaaa gcctgatccc atccccttgg cccatgtcac gatatccggc tcaacgccaa
     1441 aggactgata ggcatagagc gcacccagac gtccaaaacc ggcctgcacc tcgtcgaaga
     1501 tcagacagat atttttttca tcacaaaatt gccgcagcgc cttcaaaaat tcggggttgg
     1561 ccggtgttaa tccgccttca ccctgtatag gctcaatgat aacagcggcc gttttatctg
     1621 tggccaaggt tttaacgcta tcaatgtcat tgaaggtagc aaaatgcacc cctggtaaat
     1681 acggtgcaaa aaacggctgg gaattgcgtt tttctgtcac gctggcgcta ccataagtcc
     1741 gcccgtgaaa gctgttatga aacgcgatga tctcgttgca gtcttcgcct ttttcatcat
     1801 aagcccattt gcgggcacat tttaatgccg cttcaacgct ttcagtcccg ctattggaaa
     1861 aataaaccag gtcaaaacag cttgtttcta ccagcaactt ggccgcctta aatttttctt
     1921 ttgtggtata ggatgcttga cacgccatca ttcgtgccgc ttgctcattg atggttttga
     1981 gcatcgccgg atgcgcgtgt cctaacgatg ccaccgcaat cccagcagcg caatcaacat
     2041 atttcttgcc ggaagtatca aacgcgtagc agcccttgcc gtggtccatc accacgggct
     2101 gcggcgcata agtcttgacc agatatcgaa atgaatcatc aatgattttt tttgcatcta
     2161 tgctcatgat ttttgggcct ttctatcctg ctataagttt atgggcctgt acttcatttt
     2221 cgtaagattg acgttcgctg tctttaaaca gcatcgtgcc cggcccaaca ggctcataaa
     2281 tttctttctt aagcgcatct tggcgtaaac cgctcagcag atgaatgcgc cgcacgcccg
     2341 catccagagc cgccttgcaa ttttccagct taacctgcat cccgcccgtg gccgtgccgt
     2401 cggcgatata cccgtcaatc tgctctgcgg taataatctc cgccgtgcgc ccgccaattt
     2461 tcaccccgtc aacatcggat agaaagatga gcttatgcgc ctgcgtgcca atcgcaatct
     2521 ctgtcgcaat cgtatcagcg ttgatattgc acgtcacgcc atcgcctgtt tgcgccagac
     2581 aggtgcaagc cacaaaattg acactgcgaa acagccggtt tatgggacgc gcgtaaacat
     2641 cttcaaccgc gccagcaaag tcatcgcctt cggtcttggg tttgaggacg acacgcatcc
     2701 aactcggcgg cacacagtta aaagacagcc catcaagctt gatctttgcc atcatcgctg
     2761 aaacgctcag cgataaatcc ccgcccaaaa catgccgggc aatctccata ttggcggcat
     2821 tggttacgcg cttgccgccg attttattaa ccttaacgcc gcacttttca gcctcttcat
     2881 ccatcgcgtg cccaccgccg taaatcaaca gcacccgaat accctgcagt gtcaaatcac
     2941 gaatattcga cagcaaattc tccaatgcgc cttcgtcttc gataatttta ccgccagcct
     3001 taatgacaaa aaagttgtcc ttaaatttgc tttcataaaa agcattgata aaaaattccg
     3061 gcattttcgt tttcttgctc ataggacctc ttacgggtat agcggggtga gcgtgtctag
     3121 ccccgcggtt tcatctaaac caaacattaa attcatattt tgtaccgcgc accccgccgc
     3181 gccttttaca agattatcca gagcgccttg cacaattacg ctttgcccca caccctttac
     3241 gaatgacaaa tcgcaaaaat tcgatcctac cacgttggct agcgcaggcg tatcttgtaa
     3301 tcgaacaaac ggcgcctcgt tataagcctg ctctgccaga tccaaaggct caatatcagc
     3361 tttcaaatgc acaaaggcgc tcgcaaaaat cccgcggcta aacggccccg atgttggcac
     3421 aaagttcaac tggctttcat cgatttgcgc gctgcgcgta atctccggaa tatgccggtg
     3481 cttgttgatg ttataactct tcatattgtg gttgcgcacc gggtgatgcg ttccgtcact
     3541 tgcggatttg ccagagccgc tggaacccgt caccgccata atatcggcgc gctcaataag
     3601 tccggcgaaa ggatatagca tgagctgtgc cagcagtgca aaacaccccg gattagaaac
     3661 cgttgaggct actgcaattt tttcagcatt aaattccggc gcaccataga ccacttcttt
     3721 gagtaactcc gggtgattgt gtgccgtata atatttctca tacaccgccg cgtcatccag
     3781 ccggtagtcc gccgacagat caatcacctt cacgctgcca tgcaaatccg ccacaatatc
     3841 ctgcgcggtt ttatggggca gggcgaggaa caccacatca ctatcctccg ccaccttcat
     3901 atgatcggta ttcgtaatag ttaaatccag atgcgctaaa tgaggataca cttcgccaat
     3961 agcaacgcgc tgatgctgac gcgaagtcag gtgtacaatc tccacattag gatgcgccag
     4021 cagcatccgt agttgttcca gacctgtata tccagtaatg ccaatgatcg atgctttaat
     4081 cattttgttt ctcccgtcat tgcgaggagc gcagcgacgc ggcaatccag ttttacacat
     4141 tcttctctgg attgcttcgc tatgctcgca atgacgttgt atctataagg actaggccgc
     4201 atcctggtac tccacggccc agcgggcaat caccagatcc agccatgcat tgtccggact
     4261 aaacgcgtta taggtcatca tatttttccg ttctttcaaa atatctttcc caacctggtt
     4321 gtccgtcacc ggtccgctca tcaaatcaat cggcaaaccg atctgttcgc aaaaatcagc
     4381 catcccaatc gccccgaccg gatcacgcgc acagccaata tgaaagcgca catttttctg
     4441 aatttgcgga tcgcgcaaga tggcttcaac gccatatttc cccatcagcc cgtcaccgaa
     4501 ttcaatcata attgcatcag gattggattt tgataaatga tcaattgcgc ccttggccac
     4561 ggcaaccatc tcggcgtcag gcatctgtgc agttgatgta atcccacaat caacaaacga
     4621 cacaccgtta taaatgccgt aatcatgcat cttaaacaaa tcgcgcgccg ccccgacacc
     4681 cgtcagtttt gccccggcca gcttcatacc catccgggtc agcgtcttga caatctcaat
     4741 cgccaccgtt gtctttccgc tatccatcga agttccggtt gtaatgatta gcggcacgtt
     4801 gctcttcatg ctctgcaccg gcttgtacaa ggcggcttgc ccgatat
//
LOCUS       DAOSWR010000152         4842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11810_length_4842_cov_5.544600, whole genome shotgun sequence.
ACCESSION   DAOSWR010000152 DAOSWR010000000
VERSION     DAOSWR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11810_length_4842_cov_5.544600"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PK513_09505"
     CDS             <1..594
                     /locus_tag="PK513_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO82727.1"
                     /translation="AGVRRLEAITGEAVEKYKQDKQAAAQAVLDKLAADYAALCKDLE
                     ALGGQPEKVDLGDEEALRVANKKLQKQISDQRRKQGASASDNDIKDIGGVKFIGKVLE
                     DFPPKDLKPMADDLKKKLGSGVIALVATNDGKASIVVAVTEDLTAKINAVDLVKLGAE
                     KLGGKGGGGRPDMAQAGGPDASAANDAVTGIEKAMAG"
     gene            complement(601..1587)
                     /locus_tag="PK513_09510"
     CDS             complement(601..1587)
                     /locus_tag="PK513_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82728.1"
                     /translation="MSEKTKFNAMTNRHDFHEVVDELFAALRPQKQQKNFFNALSAQK
                     REELTTAFNRTSMARSTFLQYMEDLARLIGHKYVTDFVILDSPKDLEKAAEKMFDEDR
                     NMHDLNRGRVLFTSYEQYELFIRRFVQKKGSKKFHETWQDKITVIEGKQDDYLKKCRK
                     SGYAGAYHFDVQVACGKDQKCAELEIQMMPKDMVGTYDLSHHLYDLIKVIEYESKKRA
                     LSDAENTVLRALITANTALWDHAALQTGFIQARERPRPHYVSEQDYAESSALLTTLLG
                     RLYAKNENKRTRKERWLIAALEEAGYALNAVYHLQKQNRPNAHQDFYETPEN"
     gene            1730..2152
                     /gene="ndk"
                     /locus_tag="PK513_09515"
     CDS             1730..2152
                     /gene="ndk"
                     /locus_tag="PK513_09515"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOO82729.1"
                     /translation="MATQRTFSIIKPDATRRNLTGAINAKLEAAGLRIVGQKRIHMTR
                     QQAEGFYAVHKERPFFGELVEFMSSEPVVVQVLEGEDAVAKNREVMGATNPQEAAPGT
                     IRKEYAVSIGENSVHGSDSLENAKIEIEYFFKPEEIVG"
     gene            complement(2169..>4842)
                     /locus_tag="PK513_09520"
     CDS             complement(2169..>4842)
                     /locus_tag="PK513_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO82730.1"
                     /translation="KTQLFTLYLEAAPGVVNSIAPSIMSTKSYQMHIVSPSLLVMVFW
                     AFLLGVAGFFLAFYSLQKSEGFVFFSSYFIFGGLLFYAYGSAFYTPSALIATSLNAMV
                     VLPTIAAVFIAKRFLELHIGQDLANLLSLIDALSVGVGAGLCLLLAGFSSVLDEYLLL
                     MPSLVTMLFLCAVSMAQAQQGRYGALFLAAGFAASFAGLLSLFLAAMGAGNTGFLLSI
                     YWGLLAPQALLFVLATLQNIQMNQREEFSNVARENRAAQSLARIKQSQESADQARLLR
                     VIERERELMAELREREMQRTQEMRKAKEAADEANRAKSAFLALVSHEIRTPMNGIVGM
                     LRLLMDTNMTKEQTEYTQAIQNSGDTMMALLNDILDFEKIESGNMHLEIIDFDMVQLV
                     DDVVTLMSGHAAGKGIKIKAQIQDGFPESLKGDPTRLRQVLLNLVSNAVKFTDQGHIT
                     IQLGFEPKDDEENRRIYAISCSVRDTGIGIAEEVQKSLFNPFIQAEKSTSRKYGGTGL
                     GLAICRRLIESMGSTIQLDSKPGEGSTFSFVLTMRGGQKAFSENAHDMEQGSLPQRII
                     EPLRIMVIEDNEMNRRVLEGFLNKGKHQIIMAENAEEALDILEKTPEDQRFDVVITDI
                     RLSGMDGMNFTRALRQFEINAHACTPVIALSGNVSAEDREEYASANMNGFLAKPLDPK
                     ALEALLIKVSEDELDVPVKTEARQTRAPILNVVPPKTPSSSSDRAPIEDMITGLAFDP
                     EIELEDDFDSFQIAHDSSGQNHEEYDSSSENAEDRNLFDSALLQGLIDSLPKGQFDEL
                     LQSFLDKTDELVETLQNAKASAMGVETIYDRAHELKGMAANFGLTGVSTVAANIEAGA
                     KNADLDAALKAIEGLGDINEKAQAALKAWASSYS"
BASE COUNT         1250 a         1212 c         1155 g         1225 t
ORIGIN      
        1 gcaggcgtgc gccgcctcga ggccatcacc ggcgaagcgg tcgagaaata taaacaggac
       61 aaacaagccg ccgcgcaagc tgtgctggat aaactggccg ctgattatgc cgcgctgtgc
      121 aaagaccttg aggccctcgg cggccaacct gaaaaggtcg atcttggcga tgaggaggcc
      181 ttacgcgtcg ccaataaaaa gctgcaaaaa caaatctccg atcagcgccg caagcaaggc
      241 gcctcggctt ccgataacga tatcaaggac attggcggcg tgaaattcat tggcaaggtg
      301 ctcgaagatt tcccgcccaa ggaccttaaa cccatggccg acgatctgaa gaaaaaactc
      361 ggctccggcg tgattgcatt ggtggccacc aatgacggca aagcctccat cgttgtcgcc
      421 gtcaccgaag acctgaccgc caaaatcaac gcggtcgatc tggtcaaact cggcgcggaa
      481 aaactcggcg gcaaaggcgg cggcggccgt cccgatatgg ctcaggccgg cggtcccgat
      541 gccagcgcgg ccaatgacgc ggtgacgggg attgaaaagg cgatggcggg ttaaagcccg
      601 tcagttttca ggtgtttcat agaaatcctg atgcgcattg ggcctgtttt gtttttgtaa
      661 atggtagaca gcgttgagcg catagcctgc ctcttccaga gcggcaatta accaccgttc
      721 tttcctggtt cgcttgtttt catttttcgc ataaaggcga ccgagcaaag ttgttaaaag
      781 cgccgaggac tcggcatagt cttgttcgga tacatagtgt ggacgcggtc tttcacgggc
      841 ctgaataaaa ccggtttgga gcgcggcatg gtcccataaa gccgtattgg cagtaatcaa
      901 agcccttaga acagtgtttt cggcatcaga tagagcgcgc ttcttggatt cgtattcaat
      961 aacctttatg aggtcgtata aatgatgcga gagatcatat gtcccgacca tatctttggg
     1021 catcatttgt atttcaagtt cggcacattt ctgatctttc ccgcaagcaa cctgaacatc
     1081 gaaatgatat gcgccggcat aaccgctttt ccggcatttt ttaaggtaat catcttgctt
     1141 tccttcgatg acggtaattt tatcctgcca tgtttcatga aactttttgg agcctttctt
     1201 ttgcacaaaa cgcctgatga aaagctcgta ctgttcataa gaggtaaata aaactctccc
     1261 ccggttcaga tcgtgcatat tacgatcttc gtcgaacatt ttttccgcag ccttttccag
     1321 atctttgggg ctgtcgagga taacgaaatc agtgacgtat ttatggccta taagtcttgc
     1381 cagatcctcc atgtattgaa gaaaagtgct ccgcgccatg cttgttcgat taaacgccgt
     1441 ggtgagttct tctcgttttt gtgcggaaag agcattaaaa aagttctttt gctgtttttg
     1501 gggacgaagt gcggcaaaaa gttcgtccac gacctcatgg aagtcgtggc ggttggtcat
     1561 ggcgttaaat tttgtttttt cagacatgtg cttcaccact gtacaaaatt ttccatattt
     1621 gtagagttgt tgttttgatt ttgcaacata aaaatcccct cttgcccttc atggcctgca
     1681 cagctataac gtgcgaacat tttgaattta tagccaaaag gataaaaaca tggccacaca
     1741 gcgcacattt tcaatcatta agcccgatgc gacccgccgc aacctgaccg gcgcgattaa
     1801 cgccaagctc gaggcagcag gcctgcgcat cgttggccaa aagcgcattc acatgaccag
     1861 acagcaggcc gagggctttt acgctgtgca caaggagcgc ccgttttttg gtgagctggt
     1921 ggaatttatg agctccgaac cggtcgttgt gcaggttctt gaaggcgagg atgctgtggc
     1981 caagaaccgt gaggttatgg gcgcgaccaa cccgcaagaa gccgcgccgg gcacaatccg
     2041 caaagaatat gctgtatcga ttggcgaaaa ctccgtccac ggttctgaca gccttgaaaa
     2101 tgctaagatc gaaattgaat atttcttcaa gccggaagag attgtcggct aacgacaaaa
     2161 gcgtagctct aagaatatga cgaagcccag gcttttaatg cagcctgggc tttttcattt
     2221 atatctccca aaccttcaat agccttgaga gcagcatcca gatctgcatt tttagcaccc
     2281 gcttcaatat tggcagcaac cgtactcacc ccggttagtc caaaattagc ggccataccc
     2341 tttaactcat gcgctcggtc ataaatggtc tctacgccca tggcgctcgc tttcgcattt
     2401 tgcagggttt caaccaattc gtcagtttta tccaaaaaac tctgcaataa ctcatcaaat
     2461 tgacctttag gaagactgtc aattaacccc tgtaacagcg ctgaatcaaa aagattacgg
     2521 tcctccgcat tttcggaaga actatcatat tcttcatgat tttgaccaga agagtcatgc
     2581 gcgatttgaa aagaatcaaa atcatcctcc agttctatct ccggatcaaa agccagaccg
     2641 gtaatcatgt cctctattgg cgcgcggtcc gaggaggagg acggggtttt tgggggtacg
     2701 acgttcaaga taggagcgcg tgtctgtctt gcttcggttt tcacaggtac gtctaattca
     2761 tcttcgctca ccttgatgag cagagcctcc agcgctttcg gatcaagagg cttggccaga
     2821 aaaccgttca tgttggcgct agcgtattcc tcgcggtctt cggcgctgac attgcccgat
     2881 agagcaatca ctggcgtaca ggcatgcgca tttatttcga actgacgcaa cgcgcgcgtg
     2941 aagttcatcc catccatgcc gctcaggcgg atgtcggtaa tgacgacatc aaagcgctgg
     3001 tcttcaggtg ttttttcaag gatatctaag gcttcttcgg cgttctctgc cataattatc
     3061 tgatgcttgc ctttgttcaa aaagccttcc agcactcgcc ggttcatctc gttatcttca
     3121 atcaccataa tacgcaaagg ttcaatgatg cgctgtggga gggagccttg ttccatatca
     3181 tgggcgttct cgctaaaagc tttttgtccg cccctcattg tcaagacaaa ggaaaaagtg
     3241 ctgccttcgc ccggtttgct atcaagctgt attgtactgc ccatggactc aataagacgg
     3301 cggcaaatag ccaaccccaa acctgttccg ccgtatttgc gtgaagtgct tttttcggct
     3361 tgaataaaag gattaaagag actcttttgc acctcttcgg ctatgccaat tccggtatct
     3421 cgcacggaac aactaatggc atagatgcgg cggttttctt catcatcctt tggttcaaat
     3481 cccaattgaa tggtgatatg tccttgatcg gtaaatttaa ccgcattact gactaaatta
     3541 agtaaaacct gacgcaagcg ggttgggtcg ccctttaggc tttcaggaaa tccatcctga
     3601 atttgggcct ttatcttaat gccttttcct gcggcgtggc ctgacattaa cgtgacaacg
     3661 tcatcaacaa gctgcaccat gtcgaaatca atgatttcaa gatgcatatt gccgctttca
     3721 attttttcga agtcgagaat atcattgagc agcgccatca ttgtatcgcc tgaattttga
     3781 atggcctgcg tatattcagt ttgttctttc gtcatgttcg tatccataag caggcgcaac
     3841 atacccacaa tcccgttcat gggggtacga atttcatggc taaccaatgc caggaaagca
     3901 gacttggcgc ggtttgcttc atcggcggcc tctttggcct tacgcatctc ctgtgtacgc
     3961 tgcatttccc gttcacgcag ctcagccatc aattcgcgtt cacgctcaat cacgcgcagt
     4021 agccgggcct gatctgctga ttcttgtgat tgcttgatgc gcgcaagcga ttgcgccgcc
     4081 cggttctctc gcgccacatt tgaaaactct tcgcgttggt tcatttgaat attttgcagc
     4141 gtagccagaa caaagagcaa agcttggggt gctaataggc cccaatatat cgaaagaaga
     4201 aatcctgtgt tacctgctcc cattgccgca agaaacagag aaagtaagcc tgcaaagctg
     4261 gcggcaaagc cagccgctaa aaacagtgcg ccgtatcgcc cctgctgggc ttgcgccatg
     4321 ctcaccgcgc acaaaaaaag catggtgacg agcgagggca taaggagtaa atattcgtcc
     4381 agaacactgg agaaaccagc caagagcaga caaagtccgg ctccgacccc gacactgagc
     4441 gcatcaatca ggctcagcaa attagccaga tcttgcccga tatgaagctc taaaaaccgc
     4501 ttggcaataa aaaccgccgc aatggtaggc aaaaccacca ttgcgttgag tgatgttgcg
     4561 ataagagctg aaggcgtata aaaagccgac ccgtaagcgt aaaataaaag ccctccaaaa
     4621 ataaaatacg aactgaaaaa gacaaaacct tcacttttct gcaggctata aaacgcaagg
     4681 aaaaacccgg cgacgccaag gagaaaggcc cagaaaacca taaccaggag tgaagggctg
     4741 acaatatgca tttgatagga ttttgtgctc ataatagaag gcgcaatgct gttcacaacg
     4801 ccaggagcgg cttccagata caaagtgaaa agctgggttt tc
//
LOCUS       DAOSWR010000153         4829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11858_length_4829_cov_4.811479, whole genome shotgun sequence.
ACCESSION   DAOSWR010000153 DAOSWR010000000
VERSION     DAOSWR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_4829_cov_4.811479"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3011)
                     /locus_tag="PK513_09525"
     CDS             complement(<1..3011)
                     /locus_tag="PK513_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO82731.1"
                     /translation="MARFFIDRPVFAWVIAIIIMLAGVLSIRALPIEQYPKVAPPTVA
                     INASYPGASAETVENSVTQVIEQRLTGIDHLRYFTSSSQDGSMSITLTFEPEADPDIA
                     QVQTQNKVQGAVTQLPQEVQALGVTVTKSNDSFLLVVGVYSEDGEMSQGELGDMLVST
                     FREPISRVNGVGNVRIFGDQHAMRIWLDPAKLYSYNLTPLDVQSAVQIQNTDISAGQL
                     GGMPAAPNQQINATVKAQARLESVEDFERIVLRVNTDGSQVRLRDVATLELGSESYDR
                     IVRYKRKPAAGMAITLATGANALQTAELVKHKVAELSEYMPEGVKVVYPLDATPFIEL
                     SIESVVHTLIEAVVLVFFVMLLFLQNLRATLIPTIAVPVVLLGTFAVLLAFGFSINVL
                     TMFAMVLAIGLLVDDAIVVVENVERIMSEEDLSPKEATRKSMDQITGALVGIAVVLST
                     VFIPMAFFSGSAGAIYRQFSITIVSAMGLSVIVALILSPSLCATLLKQHKGEKRRADT
                     GFFGWFNKYFNKGRDGYQKSASFIAGRAFRFFMVYIVLVGGMMGIFMQLPGSFLPNED
                     QGTMYLMVNTPPSSTAERTLESVKQVEDYFLEQQKDTVEHLFTVVGFSFAGSAQNAAF
                     GFVGLKDWDERTQDDQSVFAIAQAAGGALYQIKDAMAFAFFPPPIRELGNSSGFDFQL
                     VDRSGIGHEALMNARNQLLGAAGQNSKLTSVRPNGLSDVPQFKIDIDSEKASALGLSL
                     SDINRTLQIAWGSSYVNDFVDKGRIKRVYLQADAPHRMMPEDLDNWYVRNNTGEMVPF
                     NTFSSAEWTYGSPKLERFNGNSSVNIQGSPAPGVSTGAAMEEIQKIVSTLPEGLGLEW
                     YGLSYEERQTGAQAPALYALSILIVFLCLAALYESWAVPFAVMLVVPLGILGAVVAAY
                     IFGLPNDVYLQVALLTTVGLASKNAILIVEFAKDLRKQGHSLMEAVSIAAKQRFRPII
                     MTSRAFMLGVTPLALASGAGAASQNAIGIAV"
     gene            complement(3017..4177)
                     /locus_tag="PK513_09530"
     CDS             complement(3017..4177)
                     /locus_tag="PK513_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013786043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO82732.1"
                     /translation="MKKIFPLLMLALLLAGGGWYWWLKNDDGTQNASSAAARPPQAVA
                     VLELKPQRITFTQQLPGRVSPYRQSQVRPQVDGIITERLFEEGADVEKGQQLYQIDDA
                     RYKAALNSAKADLASARANVKTVEARAKRYEELVKINAVSEQEYDDVKAEFDQAMAAI
                     AVAQAALDVAQVNLDYTKVYAPISGRISRSFVTEGTLVTANQEQFLATITQLDPVYID
                     MQQSGSEAIYLRSRMMGKETVSVHLMLDEKTGMDYPHEGSLKFSEVTIDETTGSIALR
                     ALIPNPDSLLLPGLFVRATLDLGQDDVLLVPQRATIRTPEGDLTVWVVDESGKAQPRP
                     VKVEQAYTDNWIVKDGLKAGDTIIVEGYQKVGPGAPVSSVPWQQSETSGGKE"
     gene            4290..>4829
                     /locus_tag="PK513_09535"
     CDS             4290..>4829
                     /locus_tag="PK513_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOO82733.1"
                     /translation="MRRTKEESEKTREDLLNAAVRIFSEKGVARSTLDEIAKAAGVTR
                     GAVYWHFENKTQIFEALHERLHRPFVDMILEDLEKDHPEPMVQLRDLCIQIMLDLEKD
                     EQRQQTLILFFMKSDYSGDLAPYKDKHREKKAESMRLFKRYIEKAKKAGKLNTQADPE
                     LLTQCISCFMKSILFEYLDN"
BASE COUNT         1249 a         1281 c         1161 g         1138 t
ORIGIN      
        1 atgacggcaa ttccgatagc attctggctg gcagcacctg cgccgctggc tagcgcaagc
       61 ggcgttacac ccagcataaa ggccctggag gtcataatga tggggcgaaa acgctgcttg
      121 gccgcgatag aaacagcttc catcaaacta tgtccctgtt tccgcagatc cttggcaaat
      181 tcaacgatca gaatagcgtt cttggatgct agcccgactg tcgtcagcaa tgctacctgt
      241 agatatacgt cattggggag accaaaaata taggccgcca caaccgcccc taaaataccc
      301 agggggacaa ccagcatcac ggcaaacggc acagcccagc tttcatagag cgcggccaga
      361 cacaaaaata cgatcaggat agacagagcg tacagcgccg gagcctgcgc tcccgtctgg
      421 cgttcttcat aagacaggcc ataccattca agacctagcc cttccggtaa ggtgctaaca
      481 attttctgta tctcctccat cgccgcgccc gtacttacgc ccggcgcagg tgacccctgg
      541 atattgacag atgaattccc gttaaaacgc tccagcttgg gcgagccata tgtccattcc
      601 gcgctggaga aagtgttgaa cggcaccatt tctccggtat tattgcgaac ataccagtta
      661 tcgagatcct ccggcatcat acggtgcggc gcatcggcct gcagatagac gcgcttaatc
      721 cggcccttat caacgaaatc attcacatag ctggatcccc atgcgatttg cagcgtccgg
      781 ttgatatcgc ttaaagacaa gccaagcgca gaggcttttt cgctatcgat atcgatcttg
      841 aattgcggca catcactcag tccattgggg cgcacactgg tgagtttaga gttttgtcct
      901 gccgcgccca aaagctgatt ccgtgcattc atcaatgctt cgtgcccaat tccactacgg
      961 tcgacaagct gaaaatcaaa accggacgag tttcccagtt cgcggattgg cggcggaaag
     1021 aaagcaaaag ccatggcatc cttgatctga tacaaagcgc cgcccgcggc ctgcgcgata
     1081 gcgaatacgc tttggtcgtc ttgcgtgcgc tcatcccagt cttttaaacc aacaaaaccg
     1141 aacgccgcgt tttgggcgga acccgcaaag ctgaagccga caacggtgaa gaggtgttca
     1201 accgtgtcct tttgttgttc aaggaaataa tcctcaacct gtttaacgga ttccagcgta
     1261 cgctccgccg ttgaactggg aggagtatta accattaaat acattgtccc ctgatcttcg
     1321 ttgggcagaa acgagccggg aagctgcata aatataccca tcatcccacc gaccagaacg
     1381 atgtatacca tgaagaagcg aaatgctcga cctgctataa aagaggcgct tttttgatac
     1441 ccgtcgcgtc ccttattaaa atatttatta aaccaaccaa agaatcctgt atccgcgcgt
     1501 cgtttctcac ccttgtgttg tttcaaaagt gtcgcgcaaa gtgaaggaga gagaatgagt
     1561 gctacgatga cagacagtcc catagccgat acaatcgtga ttgagaattg acggtaaatc
     1621 gcgccggcag agccgctgaa aaaggccatc gggataaaaa ccgtagacag cactacggca
     1681 atgccgacca gagcgcctgt aatctgatcc atggatttac gcgtggcctc cttgggcgat
     1741 aagtcttcct cgctcatgat gcgttcgaca ttttccacaa ccacaattgc atcatcgacc
     1801 aaaagcccga tagccagaac catggcgaac atagtcagca cattgattga aaacccgaaa
     1861 gctaaaagca ctgcaaaagt tcccagtaaa acaacaggaa cggcgatcgt cgggatcaag
     1921 gttgcccgca gattttgcaa aaacagcagc atcacgaaga agacaagaac aaccgcctct
     1981 atcagtgtgt gaacaacgga ttcaatggag agttcgatga aaggtgttgc atcaaggggg
     2041 tagacgactt ttaccccttc gggcatatat tccgacagtt ctgccacctt gtgcttaaca
     2101 agctcggctg tctgcaatgc attggcacct gttgcaagtg tgatggccat tcccgccgcc
     2161 ggttttcttt tatagcgcac aatcctgtcg taactttcgg agccaagttc cagtgtcgct
     2221 acgtccctga gccgtacttg cgatccatcc gtattgaccc gcaaaacgat acgttcgaaa
     2281 tcttcaacgg actccagccg tgcctgcgct ttgaccgtcg cattaatttg ctggttcggg
     2341 gcggcgggca tgccgccgag ttgacccgcc gaaatatcgg tgttctggat ttgaacggca
     2401 ctttgcacat ccaaaggggt caaattgtaa ctatagagct tggcaggatc gagccatatc
     2461 cgcatcgcat gttggtctcc gaatatacgg acatttccga ccccgttcac gcgcgagatt
     2521 ggctcacgga aggtgctaac cagcatatcg ccgagttcgc cctgtgacat ttcaccgtct
     2581 tccgaataaa cgccaacaac cagcaggaag ctgtcattgg attttgtcac ggtaacacca
     2641 agagcttgca cctcttgcgg cagttgcgtg actgcgcctt gcaccttgtt ttgcgtttgc
     2701 acctgtgcaa tatcgggatc tgcctctggc tcaaacgtca aggtgatgct catagagccg
     2761 tcctgactgc ttgaggtgaa atagcgcaaa tgatcgatcc ccgtcaggcg ctgttcaatg
     2821 acctgcgtga ccgaattttc aaccgtttcg gcggaggcac cggggtaact tgcattaata
     2881 gcgacagtgg gcggagcgac ttttggatat tgctcaatcg gcaaggcgcg gatcgataaa
     2941 acgccggcca gcataatgat aatggcgata acccatgcaa aaacggggcg gtcaataaaa
     3001 aaacgagcca taaattttac tccttaccgc ctgatgtttc gctttgctgc caaggcacag
     3061 aggagacagg cgcaccgggg ccgactttct gatagccttc gacaatgatg gtatcgccgg
     3121 cctttaaccc atcctttaca atccagttat ctgtataggc ttgctcaacc ttaacaggac
     3181 gcggctgcgc ttttccggat tcatcaacaa cccagaccgt caaatcgcct tccggcgtgc
     3241 gaattgtcgc ccgctgcggc accaggagaa catcatcctg ccccagatca agcgttgccc
     3301 gcacaaagag acccggcagc agtaaactat ccgggtttgg aatcagggcg cgcaaagcaa
     3361 tagaaccagt cgtttcatca atcgtcactt cggaaaattt caaactgcct tcatgggggt
     3421 aatccatgcc tgttttttca tccagcatta aatggaccga gaccgtctcc ttacccatca
     3481 tgcgggaacg caaataaatt gcttccgatc cggattgctg catatcgata tagaccgggt
     3541 caagctgggt tatggttgcc aggaattgtt cctgattggc tgttaccaaa gtgccttcgg
     3601 ttacaaaaga tcggctaatt cgcccggaaa tcggggcata cactttcgta taatccagat
     3661 tgacttgcgc cacatccaga gccgcctgcg cgaccgcaat agcggccatg gcctgatcga
     3721 actcggcttt cacatcgtca tattcctgct cgctcaccgc attgattttg acaagctctt
     3781 cgtagcgttt tgcgcgggct tcaacggttt ttacattcgc gcgggcgctg gccagatccg
     3841 ccttggcgct attcagcgcc gccttatagc gggcgtcgtc aatttgatac agttgctggc
     3901 ctttttcaac atccgcccct tcttcaaaca ggcgctcggt gatgattcca tccacttgag
     3961 ggcgcacctg cgattgacgg tagggcgaaa cacggccggg aagttgttgc gtaaatgtaa
     4021 ttctttgcgg ttttaattcc agaaccgcca cggcctgcgg cggccttgcc gcagcggaag
     4081 acgcattctg tgtgccgtcg tcgttcttga gccaccaata ccaaccacct ccggcgagca
     4141 ggagcgccag cattaataaa gggaatattt tcttcatatt ttgtgtttcc ttttcctgct
     4201 gtctttcgaa gctggacaaa tataaaaatt acagctatgt ttaaccatac atttatgtat
     4261 gtatgatgtc aagacaaagg ataactctta tgagacgcac gaaagaagag tcagagaaaa
     4321 cgcgtgaaga cctgttaaac gccgctgttc ggattttttc tgaaaaaggg gtggcacgca
     4381 gcacgttgga tgaaattgca aaagcagcag gcgttacacg gggcgctgtc tattggcact
     4441 ttgaaaacaa aacgcagatt tttgaggctt tacacgagcg cctgcatcgc ccttttgttg
     4501 acatgatcct ggaagacctg gaaaaagacc atccggaacc gatggtgcag cttcgggatt
     4561 tatgtatcca gatcatgttg gatctcgaaa aagatgaaca aagacagcaa acccttattc
     4621 tgtttttcat gaaaagcgac tattccggcg atcttgcacc ctataaagac aaacatcgcg
     4681 agaaaaaagc ggagagcatg cgcctgttta aacgttatat cgaaaaagcc aaaaaagccg
     4741 gaaaattaaa tacacaggcc gaccccgaac ttttaacaca gtgtatttcg tgctttatga
     4801 aaagcatcct ctttgaatat ctggataac
//
LOCUS       DAOSWR010000154         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11868_length_4826_cov_6.133934, whole genome shotgun sequence.
ACCESSION   DAOSWR010000154 DAOSWR010000000
VERSION     DAOSWR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11868_length_4826_cov_6.133934"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..909)
                     /locus_tag="PK513_09540"
     CDS             complement(124..909)
                     /locus_tag="PK513_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOO82734.1"
                     /translation="MEHWDLHKIKDRHLVYKHLVPSAWDKPKPSPVTHLILILILFSL
                     ILFTLETELSFQSKFETTLTVINMIVAVLFGIEYGLRVWTCVENPKFQGKWGRLKFAL
                     TPLALIDLVAFLPTLIFIGDTSTYWLRIVRVVRILRLLKLGRYSSSIKMVVNSIRKSW
                     RELVVTFCASLFFLYLSAVLLYFVESDAQPEGFGSIPRAIWWAVATLTTVGYGDVYPI
                     TALGKLCAGIIALIGVAIVALPAGILAGSFIEEFKNQRDRGER"
     gene            1028..2746
                     /locus_tag="PK513_09545"
     CDS             1028..2746
                     /locus_tag="PK513_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOO82735.1"
                     /translation="MAGYASAWADGVDYNFSITGIEDEKLESRLKERSLLVSLADKKP
                     ANMAALNRRIKSDLKSFKIVMQEHGYFGASLKQDITEDSGKKKVTLTIDKGALYTIEN
                     FDIEWGGEQKPDVEAISSLAFPLNQTALPETLLDAEQQLFNALMQNGYYEPQSKGKEL
                     RVHHNKQSVSVRLSIVPGPKAVFGATDITGFKRLEEPFVRRRIAWNEGEVFNIEKIAR
                     TRKKLNRSGVISNTEVQYGEVTRGNALPVTLAVKEGKHRSVGAGVAYSTTLELVGNVF
                     WEHRNLFGGAEKFRVKLEGGTQTYGLSANLNKPDLWGNEKLGWQNSVVFKRELLEAFD
                     KDTATYGTTLNYKYSGTSAISGGFTIEQNRIEEGGEDKESFTMVSVPLTYRYDNTNDA
                     LDPREGLRFNVGITPYQVLNEQNSFVVTSTGASHYLPLGENFVWANRARAAVIHGQRL
                     EDVPADKRLYAGGGGSVRGYGYQLLGPLDAENDPTGGRMAFEVGTEGRVKVTEDIELI
                     GFIEGGRVSEDMEFNSDADFFWSAGGGVRYHTPIGPLRFDLAVPLDKRDVDNEFQFYI
                     SIGQAF"
     gene            2747..>4826
                     /locus_tag="PK513_09550"
     CDS             2747..>4826
                     /locus_tag="PK513_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82736.1"
                     /translation="MKRAALKFLIWAAGTTLLLATLVILAGALGLSWLGGSESGRNWI
                     AGQISALASSEDLTVEINGIQTLNSRHVAVSSIALKDQGGAFATLKNVEIDFVAEHLL
                     AGNLHINRIGVKEASLSRLLPEGEEGKATNAFPDIPDLHIESLNIENINLAPEITGKS
                     EMLALDGTLLLSPVLHKNHISLQLEDKNTANLMADITFAPEVDDLKLQASIKDNGQGL
                     LARLSGLPLIDVSLEGAGSLENWEGQLQATTGEAFKTEGQVVLNLKAKQPVLTLAGKT
                     QYEGYKADGDATLTFDHKTEKLDFVFSGDFEEEDYALRGLSLRGQAKPEKTFFGAGHG
                     TVNLSCTGNTQNVVDLETVENIDLLTGAVFEAQGRIKGDQIDITASRIKTDRFNLTAT
                     GIVDLESNQTKLATKTDVADLSWLSSDFTGSSTIQAQISGSVEPLDLKAPVQITTQNF
                     KTVWPDVNEALGITPSAKADLSYTDKLGIKNGVLKGAKLKALNFSGTIGDSQTRLNMA
                     MNYFEHDIKMVLNIKGGDLVFDPITITGDLGKLNGNATYNLDQEKVQAALTLKPDNNN
                     TFSAKLSGQSESLSYTGDWRGEGAYPFTFKYEGAIDNQQNTVVKLSKFSGDYGPNRLA
                     LKNPAILTFKEGGAEVDNLAFGLNDGMLGAFGKFSAKEFSVTLKANNLPANLNVFPFV
                     FDGRFAGDISL"
BASE COUNT         1348 a         1011 c         1235 g         1232 t
ORIGIN      
        1 ggaaaccctc aaatactgat tctgagagac aagctcgtgt agcggttttg ttttatttgg
       61 catggttttt tccctttcat gattctatag gcatgatttt atcagaaagc attgcaagaa
      121 aaactatctt tcaccccgat cacgttgatt tttgaattct tcaataaagc ttcccgcaag
      181 gatacccgca ggcagggcga caatggctac accgatgagt gcgataatgc ctgcgcaaag
      241 tttgcctaaa gctgtgattg gataaacatc gccatagcca accgtggtta aggtggccac
      301 agcccaccag atggcccgcg gtatgctgcc aaagccctcc ggctgggcat cactttctac
      361 aaagtataaa agtacggcgc tgagatagag aaaaaacaag gaagcgcaaa aggtcacaac
      421 aagttcgcgc cagcttttgc ggatggaatt gacaaccatc ttgattgagc tagagtaacg
      481 ccccagcttt aacagtcgta aaatacgcac aacacgaaca atgcgtaacc aataggtgct
      541 ggtatcgcca atgaagatca gcgtaggcag gaaggcaact agatcaataa gggccagggg
      601 tgttagggcg aatttgagcc gcccccattt cccctgaaat ttcggatttt cgacgcatgt
      661 ccagacccgc aaaccgtatt caatgccgaa taaaaccgca acaatcatat tgatcaccgt
      721 cagcgtggtt tcgaatttgg attggaaaga cagctcggtt tccagtgtaa ataaaatcag
      781 gctgaacagg atcaaaataa ggatcaggtg agttactggc gagggttttg gtttgtccca
      841 tgcgctgggg accaagtgct tatagaccag gtggcggtct tttattttgt gtaaatccca
      901 gtgttccatt gtaagaatcc tgcatgtctc ctaataatca gactatatta acttataaat
      961 taatatagta ttttaagcca gttacatgag agcaattgcg tacgctattt tatgcagcct
     1021 tttccttatg gcaggatatg cttccgcgtg ggcggatggt gtcgattaca atttttcgat
     1081 taccggcatc gaagacgaaa aactggaatc caggctaaaa gaacgctctt tgcttgtttc
     1141 actggcggat aaaaaaccag ccaatatggc ggcactaaac aggcgcatta aatccgatct
     1201 caaaagcttt aaaatcgtta tgcaggagca tggctatttt ggcgcgtcct tgaagcagga
     1261 tataacggag gacagcggca agaaaaaagt gacgctcacc atagataagg gcgcgttata
     1321 tacaattgaa aattttgata ttgaatgggg tggggagcaa aagcctgatg ttgaagctat
     1381 ttcttccttg gcgtttcctt taaaccaaac ggctttaccg gaaaccttgc tggatgcaga
     1441 gcagcagctt tttaatgcac tcatgcaaaa cggctattac gaaccgcaat ccaaaggcaa
     1501 agaactacgc gtacatcaca acaagcagag cgtttctgtt cgcctgtcca ttgtccccgg
     1561 ccccaaggcc gtttttggtg ccaccgatat tacaggtttt aaaaggctgg aagagccatt
     1621 tgtacggcgg cggattgcgt ggaatgaagg cgaggttttt aacattgaga aaatcgcccg
     1681 cacccgcaaa aaactcaacc gtagcggcgt gatttcaaat accgaagtgc aatatggtga
     1741 ggtcacgaga ggaaacgcat taccagttac gttagctgtg aaggagggca agcatcgaag
     1801 tgttggcgca ggcgtggcct attcaacaac gctggagctg gtgggaaatg tattttggga
     1861 gcaccgtaat ctctttggcg gagcggagaa attcagggtc aaactggaag gcgggacgca
     1921 gacttacggc ttgagtgcta atttgaacaa gcctgacctt tggggcaatg aaaagctggg
     1981 ttggcaaaat tcagttgtgt ttaaacgcga gcttcttgaa gcctttgaca aagatactgc
     2041 aacctacggc acgacgctaa attataaata ctccggtaca tcggccattt ccggtggttt
     2101 tacaattgaa caaaaccgca ttgaggaagg tggcgaggat aaggaaagct tcacaatggt
     2161 ctctgtgccg ctcacctacc ggtatgataa taccaatgat gcactcgatc cacgggaagg
     2221 tttgcgtttt aacgttggga taacgccgta tcaggtttta aatgagcaaa acagttttgt
     2281 ggtcactagc acaggcgcca gccactattt accgctgggt gaaaattttg tctgggccaa
     2341 tcgggcgcgt gctgccgtta ttcatggtca gcgtttggaa gatgttcccg cagataaaag
     2401 gctctatgcg ggtggcggcg gttcggtgcg cggttatggg tatcagcttt tggggccgct
     2461 cgatgccgaa aacgacccca cgggcgggcg catggccttt gaagtaggga cggaaggccg
     2521 ggtcaaggta acagaagata tagagcttat tgggttcatt gaaggcgggc gcgtaagcga
     2581 ggatatggaa tttaacagtg atgccgattt tttctggagc gcaggcggtg gcgtgcgtta
     2641 tcacacgccc attggcccgt tgcgctttga tctggccgtg ccgctggata agcgcgacgt
     2701 ggacaatgag tttcagtttt atatcagcat aggacaggcg ttttagatga aaagagccgc
     2761 tttaaaattt ctgatatggg ccgcagggac aaccctgcta ttagctactc ttgttattct
     2821 agccggagcc ttggggcttt catggctggg tggcagcgag agcggccgca actggattgc
     2881 cggtcagata agtgccttgg caagttctga ggacttgacc gtcgagatta atggtattca
     2941 aacgctcaac agccgccatg tggctgtttc cagtattgcc cttaaagatc aaggcggcgc
     3001 atttgcgacg cttaaaaatg ttgagattga ttttgtggcc gagcatttgc tggccggtaa
     3061 cctgcatatt aatcgtattg gtgttaaaga ggcttcattg agccgtcttc tccccgaagg
     3121 cgaagaaggt aaagctacaa atgcatttcc tgatattccc gatctgcata ttgaaagttt
     3181 aaacattgaa aacataaatc ttgcacctga gatcacggga aaaagcgaga tgttggcact
     3241 ggatggaacg cttttgcttt cgcctgtact gcataaaaac cacataagtt tgcagctgga
     3301 ggataaaaat acagccaact taatggctga tataaccttt gcgccggaag tagatgattt
     3361 aaaactacag gcttcgatca aggataacgg acaaggatta ttggctcgtc tgagcggttt
     3421 acccctgatt gatgtatcac ttgaaggtgc cggctctctg gagaattggg aagggcaact
     3481 gcaagccaca accggcgaag cgtttaaaac cgaaggacaa gtggttttga atctgaaagc
     3541 aaagcagcct gtcctgactt tggcgggcaa aactcaatat gaagggtata aagccgatgg
     3601 tgatgcaacc ctaacatttg atcataagac cgaaaagctg gattttgttt tcagtggcga
     3661 ttttgaggaa gaagattatg cattgcgcgg tttatctcta agaggacagg caaaacctga
     3721 aaagacgttt tttggagcag gacatggaac tgtaaatctc tcttgcaccg gcaacaccca
     3781 aaatgttgtt gatctggaaa cagttgaaaa tattgacctt ttaacgggcg cagtttttga
     3841 ggcgcaaggg cgcataaaag gcgatcagat tgacattacg gccagccgga ttaaaacaga
     3901 tcgctttaat cttacagcaa ccggcattgt tgatttggaa agcaatcaga ccaaactggc
     3961 gacaaaaact gatgttgccg atctaagttg gctttcatcc gattttacag gctcaagcac
     4021 cattcaagcg caaatcagcg gcagcgtaga gccgcttgat ttgaaagccc ctgtgcaaat
     4081 tacaacgcag aatttcaaga ccgtatggcc tgatgtgaat gaggctttgg gtataacgcc
     4141 gagcgcgaag gctgatctgt cttatacgga taagcttgga atcaagaacg gggttttaaa
     4201 aggggcaaag cttaaggcgc tgaatttttc aggcacgatt ggtgatagtc aaaccaggct
     4261 taacatggcc atgaactatt tcgagcatga catcaaaatg gttttgaata tcaaaggcgg
     4321 tgatcttgtt tttgatccca ttacgatcac gggcgattta ggtaaactaa atgggaatgc
     4381 cacatacaat ctagatcaag aaaaagtgca ggctgcactg acgttgaagc cggataataa
     4441 caatacattt tcggccaaac tttccggcca gtctgaaagc ctgtcttata caggagattg
     4501 gcgtggcgaa ggggcatatc cgtttacctt caaatatgaa ggggcaattg ataatcagca
     4561 aaatacagtt gttaaactct ctaaattttc cggtgattac gggcccaacc gtttggcctt
     4621 gaaaaaccct gctatcctta cttttaagga aggcggcgca gaggttgata atcttgcttt
     4681 tggcctcaat gacggtatgt taggtgcttt cggaaaattt agcgcgaagg aattttcggt
     4741 cacgctcaaa gcgaataatc tgcctgccaa tctcaatgtt tttccttttg tttttgatgg
     4801 acgctttgcc ggagatataa gcctgt
//
LOCUS       DAOSWR010000155         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11920_length_4813_cov_5.299916, whole genome shotgun sequence.
ACCESSION   DAOSWR010000155 DAOSWR010000000
VERSION     DAOSWR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11920_length_4813_cov_5.299916"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /gene="rpsC"
                     /locus_tag="PK513_09555"
     CDS             complement(<1..400)
                     /gene="rpsC"
                     /locus_tag="PK513_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO82737.1"
                     /translation="MGQKVNPIGLRVGINRTWDSRWYANRDYAEKLVEDLKLREYILE
                     HLKAAGISKVIVERAAKNTKVTVHTARPGVIIGKKGADIEKLRSQLAARAGGDVSLNI
                     VEVRKPELDAQLAAEGVAQQLERRVSFRRAM"
     gene            complement(420..716)
                     /locus_tag="PK513_09560"
     CDS             complement(420..716)
                     /locus_tag="PK513_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82738.1"
                     /translation="MSELKISFRKHGKSDEFFVDIPVAGIDELIRLGEAETKERVYGD
                     VWKLVASSAPVSLIDGSEVLATSEEILNGVNGHMIQNKDGNFIRVEQQPAGTEL"
     gene            complement(755..1132)
                     /gene="rplV"
                     /locus_tag="PK513_09565"
     CDS             complement(755..1132)
                     /gene="rplV"
                     /locus_tag="PK513_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOO82739.1"
                     /translation="MGQDKNARRVKDNEAMAYGKYFQTSPQKLNLIAQTIRGKNAGRA
                     LIDLEFSNRRMAGDVRKLLQSAIANAENNHGLDVDRLVVAEASVGKTLKMKRFRARAR
                     GRAARIEKKFSNMMIVVRETEEA"
     gene            complement(1138..1416)
                     /gene="rpsS"
                     /locus_tag="PK513_09570"
     CDS             complement(1138..1416)
                     /gene="rpsS"
                     /locus_tag="PK513_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO82740.1"
                     /translation="MARSVWKGPFVEKSILKKVEEARNAGKNTVIKTWSRRSTILPNF
                     VGLTFGVHNGKKFIPVVISDQMIGHKLGEFAPTRTYHGHGADKKAKKG"
     gene            complement(1430..2254)
                     /gene="rplB"
                     /locus_tag="PK513_09575"
     CDS             complement(1430..2254)
                     /gene="rplB"
                     /locus_tag="PK513_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO82741.1"
                     /translation="MALKKYNPTSPGQRQLVQIDRKDLYKGKPEKTLTEGLTKTGGRN
                     NAGRITYRHIGGGHKRRYRMIDWKRDKWDIEGTVERLEYDPNRTAFIALITYADGEKR
                     YIVAPQRLNVGDKIIAGEKVDVKPGNAMPLRRMPVGTIIHNIEMKPGKGAQMIRSAGT
                     FAQLVGRDQGYAQVKLSSGELRVVRGDCIAVVGAVSNPDHMNTNDGKAGRQRWKGKRP
                     HVRGVVMNPVDHPHGGGEGRTSGGRHPVTPWGVPTKGYKTRKNKATDKYIIRRRKK"
     gene            complement(2318..2524)
                     /locus_tag="PK513_09580"
     CDS             complement(2318..2524)
                     /locus_tag="PK513_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82742.1"
                     /translation="MSFVKAARKTAEQLALRDIQNDAARGEGLGITDSLVAAATRDAR
                     QGEGPVECCLLGEVQQDYESHFGL"
     gene            complement(2546..2860)
                     /locus_tag="PK513_09585"
     CDS             complement(2546..2860)
                     /locus_tag="PK513_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO82743.1"
                     /translation="MAKAKKQEATVKNYNTVIAPHITEKATMGSEHGQVTFKVAKDAT
                     KPEIKEAVEALFDVKVKAVNTLIQKGKTKRFKGIMGKRSDFKKAIVTLEDGQTIDTGT
                     GV"
     gene            complement(2865..3488)
                     /gene="rplD"
                     /locus_tag="PK513_09590"
     CDS             complement(2865..3488)
                     /gene="rplD"
                     /locus_tag="PK513_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO82744.1"
                     /translation="MKIAVKTLDGKASGEITLDKDIFGAEVRQDILHQMVNYQLAARR
                     SGNHKAKTRCEVSGTGKKPWSQKGTGRARAGDLKRNIDRGGAVVHGPVVRSHAIQLPK
                     KIRQLAMKSALSSKAAQGKLIILDEAKAKDHKTKPMAAALKKLELDNALIVGGKEVDV
                     NFARATANLPRIDVLPSAGANVYDILRRDVLVLTKDAVKDITEKLKG"
     gene            complement(3492..4262)
                     /gene="rplC"
                     /locus_tag="PK513_09595"
     CDS             complement(3492..4262)
                     /gene="rplC"
                     /locus_tag="PK513_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO82745.1"
                     /translation="MSVERQRTGLIARKEGMTRIFDEDGRHVPVTVLKLDECQVVGVR
                     TEEKDGYSAVALGAGKAKIKRTSKANRGVFAKAKIEPKKKVVEFRVANENLLEIGAQL
                     SVNHFVSGQFVDVTATSKGKGFAGGMKRHNFGGLRASHGVSVSHRAHGSTGQCQEPGR
                     VFKGKKMAGHLGDERVTTQNLQIVAVDEEDGLILVKGAVPGAKQGWVLINDAVKKPAH
                     KDAPFPAGLKETAKPQAAKEEAPAEETKTEETSEKKED"
     gene            complement(4326..4652)
                     /gene="rpsJ"
                     /locus_tag="PK513_09600"
     CDS             complement(4326..4652)
                     /gene="rpsJ"
                     /locus_tag="PK513_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO82746.1"
                     /translation="MDAQSIRITLKAFDHRILDTASSEILNTAKRTGASVRGPVPLPN
                     RITKFSVIRSPHVNKNSQEQFEMRTHKRLLDVIDPTPQTIDALMKLDLPAGVDVEIKI
                     GQMQAA"
     gene            complement(4683..>4813)
                     /gene="tuf"
                     /locus_tag="PK513_09605"
     CDS             complement(4683..>4813)
                     /gene="tuf"
                     /locus_tag="PK513_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030606.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOO82747.1"
                     /translation="GDNINGMEVELIAPVAMSEGLRFAIREGGRTVGAGVVSKIVE"
BASE COUNT          930 a         1306 c         1171 g         1406 t
ORIGIN      
        1 tcatggcgcg gcggaaggat acgcggcgct caagctgctg ggcaacgcct tcggcggcca
       61 gttgtgcatc gagctcgggc ttgcgtactt caacgatgtt caaggacacg tcgccgcctg
      121 cgcgagcagc gagttggctg cgcagttttt caatgtccgc accttttttg ccgataatca
      181 cgccggggcg cgccgtgtga acggttactt tggtgttttt tgccgcgcgc tcgacaatca
      241 ctttcgagat cccggcggcc ttgagatgct caaggatgta ttcgcggagt ttaagatcct
      301 caaccagctt ttcggcatag tcgcggttcg cataccagcg gctgtcccat gtgcggttta
      361 tccctacgcg cagtccaatt ggattaactt tctgtcccat gttttctcct atatcgctat
      421 tataattccg tgcccgcagg ctgttgttct actcttataa aattaccgtc tttgttctgt
      481 atcatatgac cgttcacgcc atttaaaatt tcttcgctgg ttgccaaaac ttcgcttccg
      541 tctatgagag aaacgggagc ggagcttgca acaagtttcc agacatcacc gtacaccctc
      601 tcttttgttt cagcctctcc taaccttata agttcgtcaa ttcccgcaac agggatgtct
      661 acgaagaatt cgtccgattt tccatgtttt cgaaatgaaa tttttaactc agacattctt
      721 tactccttgt tacgcagttt aacccctttt aagtctatgc ctcttctgtt tcgcgcacga
      781 caatcatcat gtttgagaat tttttctcaa tgcgtgcggc gcggccacgg gcgcgggcgc
      841 ggaaacgctt cattttcagt gttttaccga cgctggcttc ggctacgacc agacgatcaa
      901 catccagacc gtggttgttt tccgcattgg cgattgccga ttgcaacagc ttgcgcacgt
      961 cgccagccat gcggcgattg gaaaattcca gatcgatcaa agcgcgaccg gcattcttgc
     1021 cacggatggt ttgtgcaatc aggttgagct tttgcggcga agtctgaaaa tacttcccgt
     1081 aagccatcgc ttcattatct ttaacgcggc gtgcgttttt atcctgtccc attgtcctta
     1141 acctttcttg gctttcttgt ctgcgccgtg accgtgatat gtacgggtcg gtgcgaattc
     1201 acccaattta tgaccgatca tctggtcgct gatcacaacc ggaatgaact ttttgccgtt
     1261 gtgaacgccg aatgtcaggc cgacaaagtt cggcaaaatt gtgctgcggc gtgaccaagt
     1321 tttgatcacc gtgtttttgc cggcattgcg agcttcctcg acttttttga ggatgctttt
     1381 ttcaacaaac gggcctttcc atacactgcg tgccatgtcc tagttctcct tatttcttac
     1441 gtcggcggat aatgtattta tccgtcgctt tgttcttgcg tgttttatac cctttggtcg
     1501 gtacgcccca cggcgtcaca gggtgacgac caccggatgt gcggccttca ccaccaccat
     1561 gcgggtgatc aaccgggttc atcacaacgc cgcgaacatg cgggcgtttg cctttccagc
     1621 gctgacgacc ggctttaccg tcgttggtgt tcatatgatc ggggttggat acagcgccaa
     1681 caacggcgat acagtcgcca cgaaccacgc gcagttcgcc ggaggaaagt tttacctgcg
     1741 catagccctg atcacgcccg acgagctggg cgaatgttcc ggcagagcgg atcatctgtg
     1801 cacctttgcc gggcttcatt tcgatgttgt gaatgatggt tcccacaggc atgcggcgca
     1861 agggcatcgc attgccgggt tttacatcca ctttttcacc ggcgatgatt ttatcaccga
     1921 cattgagacg ctgtggagca acgatgtagc gtttttcccc atctgcatat gtgatcagag
     1981 cgataaaggc tgtgcggttc gggtcatatt cgaggcgctc gactgtacct tcgatgtccc
     2041 atttgtcgcg cttccagtcg atcatgcggt agcggcgctt gtggcctccg ccgatgtggc
     2101 ggtaggtaat gcggcctgca ttgttgcgcc cgccggtctt ggtcaaacct tcggtgaggg
     2161 tcttttccgg cttgccctta tacagatcct tgcggtcaat ttgcaccaac tggcgctgac
     2221 ctggcgatgt ggggttatac ttctttaatg ccattttctt cttcctttat tcacccgttc
     2281 tcggcgggcc accatagtta aaattcctta tcagaggcta aagcccaaaa tgactttcgt
     2341 agtcttgttg tacctctcct aaaagacagc actctaccgg accctcacct tgccgcgcat
     2401 ctctcgtagc ggcagctaca agactgtctg taatgccgag tccttcacca cgcgccgcat
     2461 cattttggat atctctcaat gccagctgtt ctgctgtttt tcttgctgct ttaacaaaac
     2521 tcatcttttt tctcctttac tcagtttaaa ctccggtgcc tgtatcaatt gtctggccat
     2581 cttccaaggt tacaatcgct tttttgaaat cggagcgctt gcccatgatg cctttaaagc
     2641 gtttggtctt acccttttga atgagcgtat tgacagcctt gaccttgaca tcgaagaggg
     2701 cttcgaccgc ttctttgatt tcaggcttgg ttgcgtcttt ggcgacttta aatgtcacct
     2761 gaccgtgctc ggaacccatt gtcgcttttt ccgtgatatg cggcgcgata acggtgttgt
     2821 agttcttgac ggtcgcttct tgtttttttg cttttgccat ggccttaacc tttcaatttc
     2881 tctgtgatgt ccttgacggc atcttttgtc agcaccagaa catcgcggcg caggatgtcg
     2941 taaacatttg caccagcgct tggcaataca tctatgcgcg gcaggttggc ggtggcgcgg
     3001 gcgaaattga cgtccacttc cttgccgcca acgatcaagg cgttgtccag ttcgagcttt
     3061 ttcagtgctg cagccatcgg cttggttttg tgatccttgg ctttggcttc atccaggatg
     3121 atcagcttgc cttgggcagc cttgcttgaa agtgctgatt tcattgccaa ttgacgaatt
     3181 ttcttcggca attggatcgc gtgtgagcga acaaccggac catggacaac agcgccgcca
     3241 cggtcgatat tgcgcttcag gtcgcctgcg cgggcacggc ctgtgccttt ttggctccag
     3301 ggttttttac cggtgccgga cacttcacaa cgtgttttgg ctttgtgatt gccagagcgg
     3361 cgcgccgcca gttggtaatt caccatctgg tgcaaaatgt cctgacggac ttcggcgcca
     3421 aagatgtctt tatcaagcgt aatttcgcct gaagctttac catccaatgt tttgactgcg
     3481 attttcatct cttagtcttc cttcttttct gaagtttctt cagtttttgt ttcttcagcc
     3541 ggagcttctt ctttagcagc ttgaggcttg gcagtttctt tcaaaccggc gggaaacggt
     3601 gcgtctttat gcgcaggctt cttaaccgca tcattgatca gaacccatcc ctgttttgcg
     3661 ccggggactg cgcccttaac caaaatcaaa ccgtcttctt catcaacagc aacgatttgc
     3721 agattttgtg ttgtcacgcg ttcatcgcca aggtgaccgg ccattttctt gcctttaaag
     3781 acccggcccg gttcctgaca ctggccggtt gagccgtgcg cacggtggga aaccgacacg
     3841 ccgtgcgaag cccgcagacc gccgaagttg tggcgcttca tcccgccggc gaagccttta
     3901 cccttggacg ttgccgtgac atcaacaaac tgaccggaga caaagtggtt cacgctgagc
     3961 tgcgcgccga tttcgaggag gttctcattg gcaacgcgga attccacgac ttttttcttt
     4021 ggctcgattt ttgccttggc aaacacacca cgattggcct tggatgtccg cttgatcttg
     4081 gctttacccg caccgagcgc gacagcgcta tagccgtctt tttcttcggt gcgcacgcct
     4141 acaacctgac actcatcgag ctttaatact gttacaggca catgacggcc atcctcgtca
     4201 aaaatgcgag tcatgccttc cttacgtgca atcagtcctg ttctttgtct ttcaacactc
     4261 attttctttt ccttcccaga ggttggcctt aagtaaccaa cgtagagtca aaacaatcca
     4321 ttattttaag cggcttgcat ctggccaatc ttgatttcaa cgtccacgcc tgcgggcaaa
     4381 tcaagcttca tcaaagcgtc gattgtctgc ggagtggggt caattacatc taaaagtctt
     4441 ttgtgagtcc gcatttcgaa ctgctcttgc gagtttttgt tcacgtgcgg agagcgaatg
     4501 acagaaaact ttgtaatacg gttgggcaaa ggcaccggac cacgtacaga agcgccggtg
     4561 cgctttgcgg tgttcaggat ctcgctgctg gcggtatcca gaatgcgatg atcaaatgcc
     4621 tttaatgtga tccgaatgct ttgcgcgtcc ataactttgt ttccttctta cgctttaatc
     4681 acttactcga cgattttgga gacgacgccg gcaccgacgg tgcggccgcc ttcgcggatt
     4741 gcaaagcgca gaccttcgct catcgcgaca ggcgcgatca gctcgacttc cataccgttg
     4801 atgttatcgc cgg
//
LOCUS       DAOSWR010000156         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_11993_length_4795_cov_4.599367, whole genome shotgun sequence.
ACCESSION   DAOSWR010000156 DAOSWR010000000
VERSION     DAOSWR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_4795_cov_4.599367"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1066
                     /locus_tag="PK513_09610"
     CDS             386..1066
                     /locus_tag="PK513_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOO82748.1"
                     /translation="MNENTPNEHREEHGMAKTSLAGRIRRYLLTGIVVTAPITITIYL
                     TYVFLTFIDDKVSRIIPKEWYEGFYGETAIPGLGLIVALTFFIVIGWFATNFLGLLII
                     RVYEYILDRMPVIRTVYGAIKQIFETIMASQSSAFREAVMLEYPRKGVWSIGFVTGVS
                     KGEVQRVTDEETINVFVPTTPNPTSGYLLFVPKKELVYLSMTVEEAVKLVVSAGIITP
                     PDRGGNSK"
     gene            1082..1786
                     /gene="cysE"
                     /locus_tag="PK513_09615"
     CDS             1082..1786
                     /gene="cysE"
                     /locus_tag="PK513_09615"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOO82749.1"
                     /translation="MSLVEDIKSIQSRDPAAPTFLEVVFGYNGFHALLFHRLNNAIWR
                     LGLRALARFIANIGRILTGIEIHPEAKIGKRLFIDHGTGVVIGQTAIIGDDVTIYHGV
                     TLGGVGRSDQRGEKRHPTIEDGAMIGAGAQVLGDITVGKHAKVGSNSVVTNDIPEGKT
                     ALGIPARVIGGDEVARGYGMPSREEMEEVTFTIDCIVKEMGHIKRELNIEVPSPATKP
                     KKKPAAQKKPTAKKSA"
     gene            complement(1778..3871)
                     /gene="recG"
                     /locus_tag="PK513_09620"
     CDS             complement(1778..3871)
                     /gene="recG"
                     /locus_tag="PK513_09620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOO82750.1"
                     /translation="MSRPFELDALFRSLTALPGVGPRNAKLFEKLCGGPKVLDLLWHF
                     PVDFIDRRFSPAIAEAPEGRVATMEVTVTNHIPNARRNQPYRVKVRDESGVMTLTFFH
                     ARKDWIEKQLTERENVIVSGKIEYYQGNPQMVHPDIAAVEERTSLETVEPIYPLTAGL
                     TNKVVRKAMTGALGFVSKLPEWLEPEYKKRQKWDDWDVALASAHEPENMQALLSEHPA
                     RARLAYDELLANQLTLALVRHRQKRIGGRVYKCNSVLRGKLLEALPFALTRAQKRALT
                     EIDKDMHAPTRMLRLLQGDVGSGKTVVAAMAMLSAVETGAQAAIMAPTEILARQHAES
                     LKPLLEACGARFVILTGRDKGKTREVLLEQIANGPEKNGAQIVIGTHALFQEGVEFAD
                     LGLAVIDEQHRFGVHQRLMLTAKGRGTDVLVMTATPIPRTLTMTAYGDMDVSRLDEKP
                     PGRKPVETLLIPNDKLEDMIEGLARKIKDGARVYWVCPLVEESEVLDLAAAEERFDIL
                     RYRFGKVGLVHGRMKAQDKDEVMQRFAAGELDILVATTVIEVGVNVPEATIMVIEHAE
                     RFGLSQLHQLRGRVGRGRDQSYCFLVYAGPLGETAKERLSIMRQTEDGFLIAEKDLEL
                     RGAGDILGVKQSGLPVFRLANLSVHGELLAAARDDAQLIMERDPELESERGKALKTLL
                     YLFERDQAIQYLRSG"
     gene            complement(3905..>4795)
                     /locus_tag="PK513_09625"
     CDS             complement(3905..>4795)
                     /locus_tag="PK513_09625"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOO82751.1"
                     /translation="RAHDITLDEIGAHLTKGALKDKSGGWLSRVLGYLGAAFIFGGLA
                     LFITMIWDDLNSPARVIITYGPGIVAFILGIMVLKDERYEKASTPLFLKSAVLLPTGM
                     FVFLHEYADGDDAQLAAMIVFGILSLQFLLPFFKLQRTSLLFFAFLFWNSSVGILMER
                     AEVPGELIGIGLGVSIMAVAWTIDRTQYRAIAPFYYFLGSVGLLWSVFDAVEGIPVID
                     ILYLPLTIFLMLVSTRMHSRTLLLVSTFALLGFLGYYTDEYFADVTGWPIALMLMGFM
                     LIGVSAYAVKLGRQIGKKPL"
BASE COUNT         1203 a         1372 c         1110 g         1110 t
ORIGIN      
        1 agcgcaatta taagcatgag aggcgttata taggcaaaca aagacgcatt aatattgccg
       61 gacagacgtc tttctttttc ccttctgtca agaagctttg atacgcgcca gtcattggaa
      121 atcctgcttt ttttaatggc ctgaaaaggg agggaactca ttttatgctc gtatcttaag
      181 gctacctgag aatatgagaa ctaataggtg ttctcgtgaa tataataccg acagcacagt
      241 gtacaataaa aactattttc aagtctactg cctaaatcct tgttctgttc ctataatcca
      301 ctgatataat agacaaagga agcgtcatcg cattctctta ctttccccat tgattttcac
      361 tgtagggcgc ggcatgatgc gcgtcatgaa cgaaaacacc cccaatgaac atcgcgaaga
      421 gcatgggatg gccaaaacca gcttggcagg gcgcatccgg cgttacctgc taacggggat
      481 tgtcgttacc gcgccaatta caatcaccat ttacctcacc tatgtgtttc tgactttcat
      541 agacgacaag gtttcccgga tcattccaaa agaatggtat gaaggttttt atggcgaaac
      601 cgcaattcct ggccttggac ttattgtcgc gctaacattt tttattgtca ttggctggtt
      661 cgcgaccaac tttcttggtc tcctgattat tcgtgtctat gaatacatcc ttgatcgtat
      721 gcctgtgatc cgcacagtct atggcgcgat taaacagatt ttcgaaacca tcatggccag
      781 tcagtcgagc gcctttcgcg aagctgtgat gctggaatat ccacgtaaag gcgtttggtc
      841 catcggtttt gtaaccgggg tgagtaaagg tgaagtccaa cgcgttactg atgaggaaac
      901 gatcaacgtt tttgtgccca cgacccccaa cccgacttcc ggctatttgc tgtttgtccc
      961 gaaaaaagaa ctggtttatc tcagtatgac ggtcgaagag gctgtcaagc tggtggtttc
     1021 tgcaggtatt atcacgccgc cggatagagg tggaaattcc aagtaaggat aatgacgccc
     1081 tatgagttta gttgaagata taaaatccat tcagtcccgc gacccggccg cgccaacttt
     1141 tttggaggtc gtattcggct ataacggttt tcatgctttg ctgtttcatc gtctcaataa
     1201 cgccatatgg aggctggggc tgcgcgcgtt ggcacgcttt atagccaata tcggacgcat
     1261 tctgaccggc atcgaaattc acccggaagc caaaatcggc aagcgtctgt tcatcgatca
     1321 cggcaccggc gtggtgatcg gccagaccgc catcattggc gatgacgtta ctatttatca
     1381 cggcgtgacc ctcggcgggg tcgggcgttc agaccaacgc ggcgaaaaac gccaccctac
     1441 gattgaagat ggcgcgatga tcggtgccgg tgcgcaagtg ctcggcgata tcacggttgg
     1501 caaacatgcg aaggtcggct cgaactctgt tgtgaccaat gacatccccg aaggcaagac
     1561 cgcactgggt attccggctc gcgtcatcgg cggcgatgaa gtggctcgtg gttacgggat
     1621 gccgtcccgc gaggaaatgg aagaagtcac tttcaccatc gattgcatcg tcaaggaaat
     1681 ggggcatatc aagcgcgagc tgaacatcga agtcccgtct ccggccacta agccgaaaaa
     1741 gaaaccagcc gctcaaaaga agccaacggc aaagaaatca gcctgaccgt aaatattgta
     1801 ttgcctgatc gcgctcgaat agatagagca gggttttcag cgctttcccg cgttcgcttt
     1861 ccaactcagg gtcacgctcc ataattaatt gcgcatcatc gcgcgccgcc gccagcaatt
     1921 ccccgtgcac tgataaattc gccagcctga agacgggcag tccactttgc ttaaccccca
     1981 gaatatcacc cgcgccacgc aattccagat ccttttcggc gattaaaaac ccgtcctcgg
     2041 tctgacgcat aatcgacaaa cgctctttcg cggtttcccc cagtggtccg gcatatacca
     2101 aaaagcaata gctctgatcc ctcccacggc ccacgcgtcc gcgcaactgg tgaagttgcg
     2161 acagcccgaa ccgctcggca tgctcaatca ccatgatggt tgcttccggc acattgaccc
     2221 cgacctcaat caccgttgtg gctaccaaga tatccagctc tccggcggca aagcgctgca
     2281 tcacctcgtc tttgtcctgc gccttcatcc gcccgtggac aaggccgacc ttgccgaaac
     2341 gataacgcaa aatatcaaac cgttcttccg ccgcggccag atcaagcact tcggattcct
     2401 caaccagcgg gcacacccaa tacactcgcg ctccgtcctt gatctttcgt gcgaggccct
     2461 caatcatatc ctccagcttg tcattgggga tcaatagtgt ttccaccggt ttgcgtcccg
     2521 gcggcttttc atccagacgg ctgacatcca tatcgccata ggctgtcata gtcagtgttc
     2581 gtgggatcgg cgtcgctgtc atcaccagaa catcggtgcc gcgtcccttg gctgtcagca
     2641 tcaaacgctg atgcacacca aagcggtgct gctcgtcaat tacggcaagg cccaaatcag
     2701 caaactcaac accttcctga aaaagagcat gcgtaccgat gacaatctgc gcgccatttt
     2761 tttccgggcc gttcgcgatt tgctccagta aaacctcgcg cgtcttaccc ttgtcccggc
     2821 ctgttaaaat cacgaatcgt gcaccgcaag cttccagcaa tggttttaaa ctttccgcat
     2881 gttggcgtgc caaaatttca gtcggagcca ttatagcagc ctgcgcgcca gtttctacag
     2941 ccgaaagcat tgccatcgcc gccaccaccg ttttcccact gcccacatcg ccctgcaaca
     3001 ggcgtagcat ccgcgttggc gcatgcatat ctttatcaat ctcggtcaag gcccgctttt
     3061 gtgctctggt cagtgcaaag ggcagggctt ccaacaactt accacgcaaa acgctgttgc
     3121 acttataaac ccgcccgcca attcgttttt gacgatgacg caccaaagcc aatgtcaatt
     3181 gattggcgag caattcgtca taagccagtc gcgcccgcgc cggatgctcg gataacaatg
     3241 cttgcatatt ttccggctca tgggcgcttg ccaacgctac gtcccaatcg tcccatttct
     3301 ggcgtttctt atattccggc tcaagccatt ccggcaattt agaaacaaac ccaagtgcgc
     3361 ccgtcatagc cttgcgcaca accttattcg tcagtcccgc tgttaaaggg taaatcggct
     3421 cgacagtttc cagactggtg cgttcctcca ccgctgcaat atccggatgc accatttgcg
     3481 ggttgccctg ataatattcg attttgccgc tgacaatcac attttcccgc tcagtgagct
     3541 gtttttcaat ccagtcttta cgggcatgga aaaaggtcag cgtcataacg ccgctttcat
     3601 cacgcacctt aacgcgatag ggctggttgc gccgtgcatt agggatatgg tttgtcaccg
     3661 taacttccat cgtcgccacg cgcccctccg gcgcttcggc aatggccgga gaaaaccgcc
     3721 ggtcgatgaa atccaccgga aaatgccaga gcaaatcgag taccttcggc ccgccacaga
     3781 gcttttcaaa cagcttggca ttgcgaggcc ctacaccggg caaggcggtc aggcttcgaa
     3841 acagcgcatc aagttcaaac ggtcgggaca tggtctttaa gtcttacgcg atatgtcgcg
     3901 cggcttaaag gggcttttta ccgatttgcc gccccagctt caccgcgtag gcgctcaccc
     3961 cgatcagcat aaagcccatc agcatcaacg caatcggcca acccgtcaca tcggcgaaat
     4021 attcatcggt gtaatatccc aaaaagccca gcaaagcgaa agttgaaacc agaagcaaag
     4081 tccggctatg cattcgcgtg ctgaccagca tcaaaaatat ggtaagcggt aaatagagaa
     4141 tatcaatgac gggtataccc tcaacagcat caaacaccga ccacagcaaa ccgacgctcc
     4201 ctaaaaagta ataaaaagga gcaatggcgc ggtactgcgt acgatcaatt gtccaggcca
     4261 ccgccataat cgagactccc aacccaatgc cgatcaactc gccggggacc tcggcgcgct
     4321 ccatcaaaat gccgactgat gaattccaga acagaaacgc aaaaaacagc aggcttgtac
     4381 gctgaagttt gaaaaacggc aacaggaatt gtaaagataa aattccaaag acaatcatgg
     4441 ccgccaattg tgcatcgtcg ccatcggcat attcatgcag gaaaacaaac atacccgtag
     4501 gcagcaacac tgcgcttttc agaaataacg gcgtcgaggc cttttcatag cgctcatctt
     4561 tcaaaaccat aatgccgagg ataaaggcaa caatccccgg cccataggta ataatcaccc
     4621 gcgccgggct gttcagatcg tcccagatca tcgtgataaa cagcgccagc ccgccaaaaa
     4681 taaacgccgc gcccagatag cccaaaaccc ggctcaacca gccgccgctt ttgtctttca
     4741 acgccccctt ggtcagatgt gcaccgattt catccagcgt aatatcatgc gcccg
//
LOCUS       DAOSWR010000157         4790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12015_length_4790_cov_5.343400, whole genome shotgun sequence.
ACCESSION   DAOSWR010000157 DAOSWR010000000
VERSION     DAOSWR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12015_length_4790_cov_5.343400"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1241
                     /locus_tag="PK513_09630"
     CDS             84..1241
                     /locus_tag="PK513_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO82752.1"
                     /translation="MAARKPISLLKPEDNFAETWPEEPDRFTEELVDPALLWCVKQNS
                     SDITIQSDRPIYNEIHGTLYPATFRSIDAADMAVFLSKIYGPEALARLASGRDLDVSY
                     EIRPDRYTRIRFRVNITAILSKGRDAAQITMRVLPSEPPTFKDLAIEEEIIEAWAPRQ
                     GLVVITGPTGSGKTTLLAAGNRHLLERPEGCGKMLTYEAPIEYVYDTIASPRSLVSQS
                     EIPRHLPDFAKGVRNALRRKPEIILVGESRDKETINASIEASQTGHAVYTTTHTIGVA
                     NTIQRMLSSYDMNEREERAIALMETLRLIVTQALVPRVSGGRCGVREWMRFPDEVREK
                     LMDMPFTDWPNETQRMINQYGQTMQRSAEIVFEAGIIDRRHYLLLSSSTGA"
     gene            1338..1646
                     /gene="icmT"
                     /locus_tag="PK513_09635"
     CDS             1338..1646
                     /gene="icmT"
                     /locus_tag="PK513_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HOO82753.1"
                     /translation="MSAAEELENLQEKMNWHWRNSMRVVRFFAFDARAAFIGIFLLFR
                     LTSWRLWLLFIGNLVFFRYLENKGLTVPAAMRNLRAWIVGVERPGWVAVKRRKFTDYG
                     "
     gene            1784..2980
                     /locus_tag="PK513_09640"
     CDS             1784..2980
                     /locus_tag="PK513_09640"
                     /inference="COORDINATES: protein motif:HMM:NF022136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HOO82754.1"
                     /translation="MAMKLFSFYRGSDMRMAGIAFLAVLFSVTTTYAGFEWVPGQPAP
                     VVAVESAPSPAPVPPAQELLMQEPQPLNPLPYEPTPMPDSGPVIKVKSMGASSESAPI
                     SQSAQQRVILPDDAPETARSSLAINPFPQGKPAAVAPAPEIVVPPTVTANEQQSAEQA
                     VVGFGTDMPLALALQQIAPPGYAFSFGESVNPGAKVSWTGGKPWIEVMNEMIAPLGLQ
                     ASVRGKVIFVHNERHSAVAPSENLIEPSAGGQGMADPTVLLPEDMVDGMRRQNVQDPG
                     IQGKVQPQETIAALDKRALESVNDIREPQSQEQVWEAEAGDSLKQTLQKWGKTGNFNI
                     DWQAMHDFTVQSDILVAGQINKALKTLVTDGLYTTAKPTFTFVNNHDEAQSSTLIIQD
                     PQSASL"
     gene            complement(2975..4186)
                     /locus_tag="PK513_09645"
     CDS             complement(2975..4186)
                     /locus_tag="PK513_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO82755.1"
                     /translation="MTGISTLGQALTRINLINDQNIQLNNLTTQLATGKITQRFSGLN
                     NNILASKRARANFQSLDTYINNIKNADRRINQMLAAIEEFQAQAENFANSLVQFSQES
                     THQKGEVIYYDDPLTTTVEITQVGMNDSEPDIDLKTMQDLASNIFNFMGDLLNVQEGD
                     VYLLGGADSLTQPYNNNGTLDAALSTLITNWKDETLPAATNLTSDELISALRSRNVAD
                     DPYAVTDTIIGYSAALSAGNVGDVFIRVDERIEVKYTALANERAFRDIMVAASFIKNE
                     NLPPIADAYAEPYTPGDPTLADGAPGATLDDMKDNFYQVFNELVGMVNNALDDIDTIR
                     FRLESARARIEEVRRTHIADQNALQNTIDDVENADLNDVAVKINMLQIQLDASYRVTA
                     SVQQLSLVNFL"
     gene            complement(4224..>4790)
                     /locus_tag="PK513_09650"
     CDS             complement(4224..>4790)
                     /locus_tag="PK513_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOO82756.1"
                     /translation="NDFVNPDFGGDLKLIGGPFDSNGAGYGAPPATTARGAIDNGANI
                     ISALFGTYNISGAAIIDQTPVSSTNYGSETDASLPVPIPTLPFREEYLGPNADISTGL
                     IGSQRIIDFAQKMINEHSQETILINARIQDEESLRDVLETQLLDESGVNLDEELGHLI
                     VVQTAYSASARVLTAVDELFQELLNAVR"
BASE COUNT         1205 a         1096 c         1135 g         1354 t
ORIGIN      
        1 accggcgatg gcaatgaaat gcgcatcggt gatcgtgcca tacagatcac aggggttcca
       61 gagctgatga caggatctaa tgaatggcag cccgcaaacc gataagcctc ctcaaacctg
      121 aggataattt tgctgaaacc tggcctgaag aacctgatcg gtttactgaa gagttggttg
      181 accctgcctt actttggtgt gtgaagcaaa attcttccga tattacgatt caatcagacc
      241 gaccgattta caacgaaatt catggtacac tttatcctgc aacctttcgt agcattgatg
      301 cagcagatat ggctgttttt ctgtcaaaaa tctatggccc ggaagcacta gcccgtcttg
      361 cctcaggtcg tgaccttgat gtgtcttatg aaattcgacc tgatcgttat acgcggattc
      421 gcttccgtgt gaatattact gcaattttat ccaaaggtcg cgatgccgct cagattacca
      481 tgagggtttt accaagtgag ccgccaacct ttaaagattt ggctattgaa gaagaaatta
      541 tagaggcgtg ggcaccgcgt cagggtcttg tggttattac cgggccaaca ggttcaggaa
      601 aaacaacgtt gctggccgca ggaaaccgac atcttttgga acgcccagaa ggatgcggca
      661 aaatgctcac ttacgaagcg ccgatcgaat atgtttatga cacgattgca agcccgcgct
      721 ctttagtttc tcaatctgaa attccaaggc atttgccaga ttttgccaag ggtgtgcgga
      781 acgccttgcg gcgaaaacct gagattatcc ttgttggtga gtcgcgcgat aaagaaacta
      841 taaacgcctc tatcgaagcc tctcagaccg gacacgccgt ttatacgaca actcacacga
      901 tcggggttgc taataccatt cagagaatgc tttccagcta tgatatgaac gagcgcgaag
      961 aacgcgctat tgccctaatg gaaacgttac ggctcattgt aacgcaagcg ctggttcccc
     1021 gtgtgagtgg cgggcggtgc ggcgtacgag aatggatgag atttcctgat gaagtgcgtg
     1081 aaaaacttat ggatatgccc tttacagact ggccgaatga aacccagcgg atgattaatc
     1141 aatacggaca gaccatgcag cgatcggctg aaattgtatt tgaagctgga attatagatc
     1201 gccgacatta tcttttgctt tcttccagca ctggcgctta atgaatattt tgcgtttatt
     1261 tactgtacct taacatttac ctctatagaa ttttattata aactgaactt tcggtactaa
     1321 attagtgagc ttgggcaatg tcagcagcgg aagaactcga gaatctccag gaaaaaatga
     1381 attggcactg gcgcaattcc atgcgcgttg tgcggttttt tgcctttgat gccagggcgg
     1441 cgtttatcgg aatttttcta ctgtttaggc ttacaagctg gcgtttgtgg ctgcttttta
     1501 ttgggaattt agtttttttc cgctatctgg aaaataaggg tttgactgtg cctgccgcga
     1561 tgcgtaattt acgtgcatgg attgtcggtg ttgagcgtcc aggctgggtt gccgtgaaac
     1621 gacgcaaatt cacggattat ggctaatatt ttcatccacc ctcaccttag aatttcataa
     1681 cagcattgct tgtgttttgt cgcgcggcgc tttatggtcg tatgagtttt cgtgataaat
     1741 aggcttaggt taagagccga gtcagtaagt aaggatcttc gagatggcga tgaagctttt
     1801 ttctttttac cggggttcag atatgcgtat ggcgggcatc gcttttcttg cagttctgtt
     1861 tagtgtaacg acaacatatg cgggatttga atgggttccg ggacaacctg ctcctgtggt
     1921 cgcggttgag agcgccccga gtccggcgcc tgtccctcct gcacaagaac ttttaatgca
     1981 agagcctcaa cctctcaatc ctcttccgta tgagccaaca ccgatgcccg attcaggacc
     2041 tgtcattaag gttaaatcca tgggggcatc gtctgaatct gcacccatat cgcaaagtgc
     2101 acaacagcgc gttatattgc ctgatgatgc ccctgaaact gcacgctcat ccttggcgat
     2161 taatcctttt cctcaaggta agcctgcagc cgtggccccg gcacccgaga ttgtagtgcc
     2221 accaacggtt acagcgaatg aacagcagag cgctgagcag gctgtcgttg gttttggcac
     2281 ggacatgcct ttggcgcttg cattacaaca aattgctcct ccgggttatg cattttcttt
     2341 tggcgagagc gttaatccag gagcaaaggt ttcctggaca ggtggtaagc cgtggattga
     2401 ggttatgaat gaaatgattg cgccgcttgg ccttcaggcc agcgtacgtg ggaaagttat
     2461 cttcgtacat aatgaacgtc attctgccgt ggccccttca gaaaacctta ttgagcccag
     2521 cgccggaggg cagggtatgg ctgatccgac cgtcttgctt ccagaagata tggttgatgg
     2581 tatgcgtcgg caaaatgttc aagacccagg tatacagggc aaagttcaac cgcaagaaac
     2641 aattgctgcg ctggacaaac gggcgctgga atctgttaac gatattcgtg aacctcaaag
     2701 tcaggaacaa gtctgggaag ctgaagcggg tgacagtcta aaacaaactc ttcaaaaatg
     2761 gggtaaaacg ggcaacttta atattgattg gcaagctatg catgatttta cggttcaatc
     2821 cgatatcctt gtcgccggac agatcaataa agcccttaaa acccttgtca ccgacggtct
     2881 atatactacg gcgaaaccaa cttttacttt tgtgaataat catgatgagg cgcaaagctc
     2941 tacgctgatt attcaagacc cgcaaagcgc ttctttataa gaagtttact aagcttaatt
     3001 gctggacact ggccgtaaca cgataagatg cgtcaagttg tatctgtaac atgttaattt
     3061 taactgcaac gtcgtttaaa tcagcatttt caacgtcatc aattgtgttt tgcaatgcat
     3121 tttggtctgc tatatgcgtt cggcggacct cttcgatgcg cgcacgtgca ctttccaagc
     3181 gaaatcttat tgtatcaatg tcatccagcg cattattcac catgccgaca agctcattaa
     3241 acacctgata gaagttatcc ttcatatcat caagggttgc gcccggcgct ccgtcagcca
     3301 acgttgggtc gcccggtgtg taaggctccg catatgcgtc ggcaataggc ggtaaatttt
     3361 cgtttttgat aaatgaggcc gcaaccataa tatctcgaaa tgcccgttca ttggccaacg
     3421 cggtgtattt cacttctata cgttcatcaa cacgaataaa aacgtcgcca acattacctg
     3481 cgctaagtgc ggctgaataa ccaatgattg tgtccgtcac ggcgtaagga tcatccgcca
     3541 cgttgcgtga gcgcaatgcg ctaatcagtt cgtcgcttgt taaattcgtt gctgcaggca
     3601 gtgtttcgtc cttccagttt gtgatcagag tgcttagcgc cgcatccaat gttccattgt
     3661 tgttataagg ttgcgttagt gaatctgcgc cgcctagaag atagacatcg ccttcttgta
     3721 cgttgagtaa atctcccata aagttaaaaa tgtttgaagc cagatcctgc attgttttta
     3781 gatcaatatc cggttcagaa tcattcatac cgacttgcgt tatttcaact gtggtcgtaa
     3841 gtggatcgtc gtaataaatg acctcacctt tttgatgagt actttcctgt gaaaactgaa
     3901 cgagagaatt agcaaagttt tcggcctgcg cctgaaattc ttcgattgca gccagcatct
     3961 gattgatgcg tcgatccgca ttttttatat tgttgatata ggtgtccaga gactgaaaat
     4021 tggcccgagc acgctttgaa gccagaatgt tattgttcaa cccgctgaaa cgctgggtaa
     4081 ttttacccgt agcgagttgt gtagtcaggt tattcaactg gatattctga tcgttgatta
     4141 aattaatgcg ggtcaatgcc tgtcctaatg ttgaaatgcc cgtcattttt tgctccttaa
     4201 tctttcaaat ttttgtaaat gccttatcgc accgcgttta aaagttcctg aaataattca
     4261 tctacagcgg tcagtacccg cgccgacgcg ctataggccg tttgaacaac tatcagatgt
     4321 ccgagttctt catcaaggtt cacccctgat tcgtctaaaa gctgcgtttc caaaacatcg
     4381 cgcaaagact cttcgtcctg aatccgcgca ttaattaaaa tggtttcctg ggaatgctca
     4441 ttaatcatct tttgtgcaaa atcgataatt cgttgcgaac caatcaaccc agttgatatg
     4501 tctgcattcg gacctaaata ttcttcacgg aatggcaatg tcggtattgg cacgggcagt
     4561 gaagcgtcag tttccgatcc gtaattcgtg cttgaaaccg gcgtctggtc aatgatggcc
     4621 gcgccggaaa tattataggt tccgaacaat gcggaaatga tattcgcccc gttatctatt
     4681 gcgccgcgcg ctgtcgttgc cggtggtgcg ccgtagcctg cgccattgga atcaaacggc
     4741 ccgcctatta attttaagtc tccgccaaag tctggattta cgaagtcatt
//
LOCUS       DAOSWR010000158         4760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12111_length_4760_cov_5.400638, whole genome shotgun sequence.
ACCESSION   DAOSWR010000158 DAOSWR010000000
VERSION     DAOSWR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12111_length_4760_cov_5.400638"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK513_09655"
     CDS             <1..480
                     /locus_tag="PK513_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOO82757.1"
                     /translation="IIFEGQEGADDISARGIHSTHLYADGRLRDLLYTNGGTSSTRTA
                     GHIVMQGREVFKYAVSFMADVVNEALEENNITADQIDWLVPHQANIRILEATAKKLGL
                     PMDKVVTTVDHHGNTSAASIPLALDEAAADGRIKQGDLLLLEAMGGGLTWGSALVRF"
     gene            627..923
                     /locus_tag="PK513_09660"
     CDS             627..923
                     /locus_tag="PK513_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOO82758.1"
                     /translation="MDNRTITRADLAEAVYEEVGLSRNESSDLVEAVIDEISDELVKG
                     ENVKISSFGSFSVREKGERIGRNPKTGVDVPISPRKVLVFRASHVLKDRINSGE"
     gene            960..1511
                     /locus_tag="PK513_09665"
     CDS             960..1511
                     /locus_tag="PK513_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO82759.1"
                     /translation="MNQVYSQKGPIVERDEQAEDLAHETAEKITGPLTGKEVERGPVK
                     QPKTRKSASAFRTISEVADILDVQQHVLRFWETKFSQIRPLKRGGGRRYYRPEDLELL
                     KRIHHLLYTEGYTIKGVQKLLKEKGKAGLLEDKPEAQNNNEPSVSASVKGQLAMGMSS
                     KQRSALQNMVNELKELRKILKSE"
     gene            1600..1676
                     /locus_tag="PK513_09670"
     tRNA            1600..1676
                     /locus_tag="PK513_09670"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1634..1636,aa:Pro,seq:ggg)
     gene            1725..1801
                     /locus_tag="PK513_09675"
     tRNA            1725..1801
                     /locus_tag="PK513_09675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1759..1761,aa:Pro,seq:cgg)
     gene            1973..2284
                     /gene="cutA"
                     /locus_tag="PK513_09680"
     CDS             1973..2284
                     /gene="cutA"
                     /locus_tag="PK513_09680"
                     /inference="COORDINATES: protein motif:HMM:NF041095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOO82760.1"
                     /translation="MIFVYVTYPSEQDAAKISKALLEDRLVACANIFPAHRSLYWWEG
                     VIQDETEVAVIYKTRRELFEPVEKAIKSKHSYDVPCIVSLPVEKAQQDFVSWMREQVR
                     T"
     gene            2397..2861
                     /gene="rplM"
                     /locus_tag="PK513_09685"
     CDS             2397..2861
                     /gene="rplM"
                     /locus_tag="PK513_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO82761.1"
                     /translation="MSKTTSAKPTEVEKKWIIVDAQDVVLGRLASIIALRLRGKHKPS
                     YTPHVDDGDNVIVINAEKVRLTGKKREDDIFYWHTGYPGGIKQRSKGQILDGQHPERV
                     IEKAVQRMLPKGPLGYKVFSNLKVYAGSEHPHEAQKPEVFDVASLNPKNKRG"
     gene            2867..3409
                     /gene="rpsI"
                     /locus_tag="PK513_09690"
     CDS             2867..3409
                     /gene="rpsI"
                     /locus_tag="PK513_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOO82762.1"
                     /translation="MAKKETEKKDEAVKDLKDLGDAVKVEDVKAEASAADKKPEEAPA
                     KRVQKLDKQGRAYATGRRKDAVARVWIKPGSGKVTVNGRDQATYFARQTQRLILNQPF
                     LIVNSVGKFDVVCTVKGGGLSGQAGAVRHGISRALENFAPEMRPALKKAGMMTRDARI
                     VERKKVGKHKARRTKQWAKR"
     gene            complement(3646..>4760)
                     /locus_tag="PK513_09695"
     CDS             complement(3646..>4760)
                     /locus_tag="PK513_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO82763.1"
                     /translation="TPGHVVYENDLMQLIQYEPQTHGVFKRPLLIVPPWINKFYILDL
                     QPQSSFIRWAVEQGHTVFCISWVNPDAKLAQKGFDAYMFEGLIAALDEIKKITGEPDC
                     NAVGYCLGGTLLATTLAWLKAKKQEKRIASTTFLTTLVDFQDSGDMKLFMDDEQIDEM
                     IEMMSRHGVLKAEELQRTFSLLRANDLIWSFVVNNYLMGKEPFPFDLLYWNDDSTNMP
                     AKMHSFYLQNMYRDNLLSHPGGIEIDGIKIDVSKVKTPAYFLSTKEDHIAPWKATYET
                     TQMLSGPKTFTLAASGHIAGVINPPSKEKYCFWSANDTPKSPEDWLKNAEETAGSWWP
                     HWQEWIKHYAGGQVEARHIKAPIEPAPGRYVQVKSN"
BASE COUNT         1153 a          944 c         1343 g         1320 t
ORIGIN      
        1 attatttttg aagggcagga gggtgcggac gatatttccg cgcgcggcat tcactctaca
       61 catctttatg cagatggcag gcttcgtgat ttactatata ccaacggcgg gacctcaagt
      121 acgcggactg cagggcatat cgttatgcag gggcgtgaag tgtttaaata tgccgtgtct
      181 tttatggctg acgtggtgaa tgaagcgctg gaagagaata atattaccgc tgatcagatc
      241 gattggctgg tgccgcatca ggccaatatc cgcattttag aggctacggc gaaaaagctg
      301 gggttgccca tggataaggt tgtgacgacg gtcgatcatc atgggaatac gtcggcggcg
      361 tccattcctc tggcgttgga tgaagccgcc gccgacgggc ggatcaagca aggggatctt
      421 ttgttgctgg aagcaatggg tggtggtttg acctggggca gcgctctggt gcgtttctaa
      481 gtcttgatcc tgctggtgca ttcttaagtc tttaggctag ccctttgaag aagaatcatt
      541 ttttggggtt aagttctttc caaatattga cgcgctggtt ttaggtcgtt agtctgagct
      601 tattcatgaa tttggagtag aaatcgatgg acaatcgtac gattacacgc gcggatctgg
      661 ctgaagccgt ttacgaagaa gttggattat cacgtaatga atcatctgat ctggtggaag
      721 ctgtgatcga tgaaatttcc gatgaattgg tgaagggcga gaatgtaaaa atttcatcct
      781 ttggcagttt ttctgttcgg gagaagggtg agcggattgg ccgcaatccc aaaaccggtg
      841 ttgatgttcc gatttcacca cgtaaagttt tggtgttccg ggcgtctcat gtattgaaag
      901 accgcattaa tagtggcgag tagtcaatta tagttaaata gtcataagga attttaggta
      961 tgaatcaggt ctattcgcaa aaaggtccaa ttgttgaacg cgatgagcag gcggaggatc
     1021 ttgcgcatga aacggctgaa aaaattaccg gtcctttgac aggaaaagaa gtggagcgcg
     1081 gccctgttaa gcagcctaaa acgcgtaaat cggcttctgc gtttcggacg attagtgaag
     1141 tagcggatat tctggatgtg cagcaacatg tgttgcggtt ctgggaaact aaattttcgc
     1201 aaattcgtcc actcaaacgt ggcggcgggc ggcgttatta tcgtcccgag gatctggagc
     1261 ttttgaagcg gattcatcat ttgctttata cggaaggtta tacaatcaaa ggcgtgcaga
     1321 agcttttgaa ggaaaagggt aaggccggtt tgttggaaga caaacccgaa gcgcaaaata
     1381 ataatgagcc aagcgtgagc gcgtctgtga agggacagct tgcgatgggg atgtcttcga
     1441 agcagcgcag tgctttgcaa aatatggtta atgaacttaa agaacttcgt aaaatactta
     1501 agtctgaata aggcttgagg gctgtggctc ctggtaattc gcgtttgctt tttcctgagc
     1561 aatcatatag aaaaatcatt tgcaagtttt ggccatgatc ggagtgtagc gtagcttggt
     1621 agcgcgttcg gctgggggcc gaaaggtcgt gggttcaaat cccgccactc cgaccatttt
     1681 ttgcatagca ctcaacttaa tgtaaaatgg gtgtgtttta tcggcgggct gtagcgcagt
     1741 ctggtagcgc gcttcgttcg ggacgaagag gtcggaggtt caaatcctct cagcccgacc
     1801 atccactttt attttgtgtt ttcttggttt tttgatggct atagctatgg tttttgctct
     1861 tggacatatt aagattgaag atccggtgtt cctggcgccg atgttgggta tttcggattt
     1921 gtcgtttcgc cggattgacg attttttttg aagcaggaga aaggttggcg gcatgatttt
     1981 tgtttatgtg acatatccat ctgaacagga tgcagcgaaa atatccaaag ctttgctgga
     2041 ggacaggctg gtggcgtgcg cgaatatatt tccagcgcat cgttcgctgt attggtggga
     2101 gggggttatt caggatgaga ctgaggtggc ggtaatttat aaaacgcgcc gggagttgtt
     2161 tgagcctgtt gagaaggcga ttaagagtaa acattcttat gatgtgccct gtattgtttc
     2221 tttgccggtg gaaaaggcgc agcaggattt tgtgagctgg atgcgggagc aggttcgaac
     2281 ttaagatatt ggtataataa taccgtaatt ttatccttct gtttttattc atttttattg
     2341 acatgtcggt gtatttgcgc gataaccacc cgactttttc aataaggaat aaaattatga
     2401 gcaaaactac atctgcaaaa cccacagaag tcgagaaaaa gtggatcatt gttgatgcac
     2461 aggatgttgt gctgggccgt ttggccagca tcatcgcgct tcgtttgcgt ggaaagcata
     2521 agccaagtta tacgccgcat gttgatgacg gcgataacgt gatcgtgatt aatgccgaga
     2581 aggtgcgctt gacgggtaag aaacgtgaag atgacatctt ttactggcat acaggttacc
     2641 ctggtgggat caagcagcgc agtaaggggc agattctcga tggtcagcat cctgagcgcg
     2701 ttattgagaa ggccgtgcag cgaatgttgc cgaaagggcc tttgggctat aaggttttca
     2761 gcaatctgaa agtctatgcc ggtagcgagc atccgcacga ggcgcaaaag cctgaagtgt
     2821 ttgatgttgc atcactgaac cccaagaata agagaggcta agcgatatgg ccaagaaaga
     2881 aactgaaaag aaagacgaag ctgtgaaaga tcttaaagat ttgggcgatg ctgtgaaagt
     2941 tgaagatgtc aaagctgaag cttctgcagc ggataaaaag cctgaagaag cgcctgcaaa
     3001 gcgtgtgcaa aagctggata agcagggtcg cgcttatgcg accgggcgcc ggaaagatgc
     3061 tgtggcgcgt gtctggatca agcccggtag tggtaaagtg accgtgaatg gtcgtgacca
     3121 agcaacttat tttgcgcgtc agacgcagcg tttaattttg aatcaaccgt tcctgattgt
     3181 gaacagcgtt ggcaagtttg atgtagtttg cactgtgaag ggcggcggtt tgtccggtca
     3241 ggcgggcgct gtacgtcacg ggatttcccg cgcgcttgag aattttgccc ctgaaatgcg
     3301 tccggccttg aaaaaagctg gtatgatgac ccgcgatgcg cggattgttg agcgcaagaa
     3361 ggttggcaag cacaaagcgc gtagaacgaa acaatgggct aaaagataat aaatttgctc
     3421 gtacgctgcg ttatcctgcg gtctcggtgt gctcacgtat tttatatacg ctccgcgcac
     3481 ctcgccttgg atgcctagcg tacaaacaaa ttgtattact tttacgcttt ataagaattt
     3541 gtgatctcct ctaccaaagt tcacaaaaca agaaacgctc cgggtaatct taatgatttt
     3601 tcggggcgtt ttttgtacgt ttaccggtta ttataggtcc cgaccctagt tgctttttac
     3661 ctgcacataa cgtcccgggg cgggttcgat gggtgctttt atatggcggg cttcgacctg
     3721 gccgccggcg tagtgtttga tccattcctg ccagtgtggc caccatgatc cggcggtttc
     3781 ttcagcattt ttaagccagt cttcggggct tttcggtgtg tcgtttgcgc tccagaaaca
     3841 atatttttct tttgacggtg ggttgataac cccggcgatg tggccggagg cggccagcgt
     3901 aaaagttttt ggaccgctca acatttgtgt ggtttcgtaa gtcgctttcc acggggcaat
     3961 gtgatcttct ttcgtggaaa ggaaataggc cggggttttg accttgctta cgtcaatttt
     4021 tatgccgtca atttcgatgc cgccggggtg gctgagcaaa ttgtcgcgat acatgttttg
     4081 caagtaaaag ctgtgcattt tggcaggcat attggtggag tcatcgttcc aatacagcag
     4141 gtcaaatggg aaaggctctt tgcccatcag gtagttattc accacgaagg accatataag
     4201 atcgttagcg cgtagcagag agaaggtgcg ttgcagttct tctgctttga gaacgccgtg
     4261 gcgactcatc atttcgatca tttcatcaat ttgttcatca tccatgaaca gcttcatatc
     4321 gccagaatcc tggaagtcga ccagagtggt cagaaaggtt gtagaagcga tgcgtttttc
     4381 ctgttttttg gctttgagcc aggccaacgt tgtggccagc agcgttccgc cgaggcaata
     4441 accaacggcg ttgcagtcgg gttcgccggt gatttttttg atctcatcga gggcggcgat
     4501 tagcccttcg aacatatagg cgtcgaaacc tttttgcgcc aattttgcat cagggttgac
     4561 ccacgagatg caaaagaccg tatggccttg ttcgactgcc cagcggataa aggagctttg
     4621 tggctggagg tccagaatat aaaacttatt gatccagggg ggcacaatca aaagcgggcg
     4681 tttgaatacg ccgtgcgttt gcggttcata ctggatcagt tgcatcagat cgttttcata
     4741 aacgacatgc ccgggggtgg
//
LOCUS       DAOSWR010000159         4704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12335_length_4704_cov_5.312110, whole genome shotgun sequence.
ACCESSION   DAOSWR010000159 DAOSWR010000000
VERSION     DAOSWR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12335_length_4704_cov_5.312110"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK513_09700"
     CDS             complement(<1..452)
                     /locus_tag="PK513_09700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOO82764.1"
                     /translation="MKLEFEVTDGCRALLPYGVSVVVPCFNAGDYLESAVSSVLRQNL
                     AHDEFEVIIINDGSTDEETLKCLDRIEARNNANVRGLHQENKGQSATRNRGIREANFA
                     YIAGLDADDMYASDPKFLGKYGLYLQRAAQIGVDEHVIRRYVERQGHR"
     gene            complement(510..3398)
                     /locus_tag="PK513_09705"
     CDS             complement(510..3398)
                     /locus_tag="PK513_09705"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOO82765.1"
                     /translation="MTQSSENRTFLSGPNSEYIAHLYGQFLADPHMVDASWRDFFDGL
                     DDSETALLQELGGASWTPPENSKAARRFGVAVEDPANDSDAPRRRAGDRPSHEESKQA
                     ASDSIRALMLIRSYRMRGHLMADLDPLEMREKTFHPELEPSHYGFTPSDMNRPIYIGG
                     VLGLEYASINEIVGVLQETYCGKIGVEFLHLSDPNEKQWVQERIEAPHNRTEFTVNGK
                     RAILQRLIAAEHFEQFLHKKYPGTKRFGVDGGEALIPLMEQIMKRGGQLGLEEIVIGM
                     AHRGRLNVLANVMGKPFTTIFAEFQGHSSTPGDVLGSGDVKYHLGTSSDRDFDGNNIH
                     LSLTANPSHLEFVNPVVIGKARAKQIQRGGVESTSVLPLVLHGDAAFAGQGVTAETLM
                     ISELSGYRVGGTIHIVINNQIGFTTMPKFSRSGPYCTDVAKMLSVPIFHVNGDDPEAV
                     VHVARIATEFRQKFKRDVVIDMYCYRRYGHNEGDEPAFTQPLMYKKIADQVPTRKKYA
                     NQLVEERVITEEEGEAMVNEFDAYLEEAFQATKDYAPNAADFLGGAWEGLKVASGDAR
                     RGETGISAEELQAIGKTLITVPEGFAINRKLQRVLDAKTKMLETGEGFDWATAEALAF
                     GSLLEDGYAVRLSGQDVGRGTFSHRHAIWYDQENEEKHIPLKHIAPDQPRFEAHDSPL
                     SEMAVLGFEYGYSLADPKTLTLWEAQFGDFANGAQVMIDQFISSGESKWLRMSGLVLL
                     LPHGFEGQGPEHSSARPERFLQMASEDNWQVCNCTTPANYFHALRRQMLRDFRKPLIM
                     MTPKSLLRHKLAVSDIDMFTDGSTFHRFLWDSDQDKLVPPEKIKRVILCTGKVYYDLL
                     VERRDREIDDVLLLRLEQIYPFPQSALAEELKPYKNADVVWCQEEPENQGYWFFVDRR
                     IEKALGEAGGKAKRPSYVGRKSASAPATGLMSRHMQEQAKLVDEALSV"
     gene            complement(3477..4025)
                     /locus_tag="PK513_09710"
     CDS             complement(3477..4025)
                     /locus_tag="PK513_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82766.1"
                     /translation="MSSLSDFSDTDRALLVCLPYKVGVFVSYADDEGGERDDKQEMDR
                     LEECIMAIAGLHISKPFTAEIMRETVEMREQWSQWAAQSWHVPEEAEQAVKVLKSRVS
                     DEELKNYRAALMEIATTVAQAYGEFGEFDDPDEGGGIFGSILAKVTKNLSSLTQDDAD
                     HPMNISAAEDSAISQLRTALTL"
     gene            complement(4036..4596)
                     /locus_tag="PK513_09715"
     CDS             complement(4036..4596)
                     /locus_tag="PK513_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82767.1"
                     /translation="MSVLDQLSAQQRTLIVSLPYRAGLWVSYSDDEGGEEAHQKELTA
                     LANLIDGFSQQVFGSELLQYIMCETLKRKDEWVNWSGDLEKIPGECAEALQVLSAHVD
                     EKDVKVYAMRLMELGEAVAVAFREHDKPSGAEKFKLFLAYALTVLKARLKKKPVKSFE
                     QFLNISTDERRALDRLASALKVDYSI"
BASE COUNT         1247 a         1443 c          935 g         1079 t
ORIGIN      
        1 tcccggtgtc cttgacgttc tacataacgg cgaataacgt gctcatcaac gcctatctgc
       61 gcagcccttt ggagatataa tccatatttc cccaaaaatt tcggatcact tgcatacatg
      121 tcatcagcat ccagccctgc tatatatgcg aagttcgcct cccgtatgcc ccgattacgc
      181 gtagcagatt gccccttgtt ttcctgatgt aaacccctca cattcgcgtt gttacgagct
      241 tcaattctat ccaaacattt cagggtttct tcgtcagtag agccatcatt aataataatg
      301 acttcaaact catcatgcgc caaattctgc cgaagaaccg aactcacagc actctcaaga
      361 tagtctccgg cattaaaaca aggaacaacg acacttacac catagggaag tagcgcccta
      421 caaccatccg tcacttcgaa ttcaagcttc atttcacaat ccttaagcta agattatgaa
      481 aaataacgca tcaatagggt aattgcaaac taaacgctta gcgcctcatc gaccagcttc
      541 gcctgctctt gcatatgccg gctcatcaac cccgttgccg gcgcggacgc agacttacgc
      601 ccgacatagc ttggccgctt cgccttgcca ccagcctcac ccagagcttt ttcaatccgc
      661 cggtcaacaa agaaccaata accctggttt tccggctctt cctgacacca taccacatcg
      721 gcatttttgt aaggcttaag ctcttcggcc agcgcgcttt gcgggaacgg ataaatctgc
      781 tccagacgca aaagcaaaac atcgtcgatc tcccggtccc gccgctcaac cagcaagtca
      841 taataaacct tcccggtgca taaaataacg cgcttgattt tttccggcgg aaccagttta
      901 tcctgatcac tatcccacaa gaagcggtgg aaagtgctgc cgtcggtaaa catatcgata
      961 tccgacaccg ccagcttatg ccgcaacaag gatttcggcg tcatcataat caaaggcttg
     1021 cggaaatcac gcagcatttg tcggcgcagc gcatgaaaat aattcgccgg cgtggtgcag
     1081 ttacacacct gccagttatc ttccgacgcc atttgtaaga accgctcagg ccgcgccgag
     1141 gaatgctcgg gcccctgccc ttcgaacccg tgcggcaaaa gcaaaacaag acccgacatg
     1201 cgcagccatt tactctcccc ggaggaaata aactggtcga tcatcacctg cgcgccattc
     1261 gcaaaatcac caaactgcgc ttcccacaat gtcagggttt tcggatcggc cagcgaatac
     1321 ccatattcaa accccagcac agccatttcg gacaaaggtg aatcatgcgc ctcaaaacgc
     1381 ggctggtccg gcgcaatatg tttcaacgga atatgctttt cctcgttttc ctgatcatac
     1441 cagatcgcat ggcggtgcga gaacgtcccg cgcccgacat cctgaccgga taaacgcacg
     1501 gcataaccat cctcaagcaa tgaaccaaac gccagcgctt cggccgtcgc ccagtcaaac
     1561 ccttcaccag tttccagcat cttggtctta gcatcgagca cacgttgcaa tttgcggtta
     1621 attgcaaacc cctccggcac ggttatcagc gtctttccaa tcgcctgtaa ttcctctgca
     1681 gaaatccccg tttctccacg acgcgcatcc ccagacgcca cttttagacc ctcccaggca
     1741 ccgccgagaa aatcagccgc attcggcgca taatctttcg tagcttgaaa cgcctcttcg
     1801 agataggcgt caaactcgtt caccatagcc tcgccctcct cctcagtgat aacgcgctcc
     1861 tcaacaagct gattggcata tttcttacgt gtgggcacct ggtcggcaat ttttttgtac
     1921 atcaaaggct gagtaaaggc cggctcatcg ccttcattgt gaccatagcg gcgatagcaa
     1981 tacatatcga tcaccacatc acgcttgaat ttctgacgaa actcggtcgc gatccgcgcc
     2041 acatgcacca cagcttccgg atcatcgcca ttcacatgga aaatcggcac cgagagcatc
     2101 ttcgccacat ctgtgcaata cggaccggag cgcgaaaatt tcggcatcgt cgtaaaaccg
     2161 atctggttgt taatcacaat gtgaattgtc ccccccacgc ggtaaccgga aagctctgaa
     2221 atcatcagcg tttccgccgt cacgccttgc cccgcaaacg cggcatcgcc gtgcaaaaca
     2281 agcggcaaca cactcgtaga ctctaccccg ccgcgttgaa tttgcttcgc gcgcgccttg
     2341 ccaatcacca ccgggtttac aaactcaagg tgcgacggat tcgccgtcaa ggacaggtga
     2401 atattattac cgtcaaaatc gcgatcgcta gaagtcccaa ggtggtattt cacatcaccg
     2461 gaaccaagca catcgccggg cgttgaagaa tgcccctgaa actcggcaaa aattgttgta
     2521 aacggttttc ccatcacatt ggccagcaca ttcaaacgac cccggtgtgc catcccgatc
     2581 acgatttcct ccagaccaag ctgcccgccg cgcttcataa tctgttccat caacggaatc
     2641 aaagcctctc cgccatcaac cccaaagcgc ttcgtgccag gatatttttt atgcaggaat
     2701 tgctcaaaat gctccgccgc aatcaaacgc tgtaaaatcg cgcgcttacc attcaccgta
     2761 aattcagtgc ggttatgcgg cgcctcaatg cgctcttgca cccattgctt ttcattcgga
     2821 tcagaaagat gcaaaaattc aacaccgatt ttcccgcaat aggtttcctg caacacccca
     2881 acaatttcat tgatagacgc atactccagc cccaaaaccc cgccgatata aatcggccgg
     2941 ttcatatcag atggcgtaaa accgtaatgc gaaggttcaa gctccggatg gaatgttttc
     3001 tcccgcattt ccaacggatc gagatcggcc atcaaatgcc cacgcatgcg ataagagcgg
     3061 atcaacatca aagcacgaat ggaatcgctc gcagcctgct ttgattcctc atgcgaaggc
     3121 ctgtcaccag cgcggcgccg cggcgcatca gaatcattcg ccggatcctc taccgcaacg
     3181 ccaaaccgcc gcgccgcttt tgaattttcc ggcggcgtcc agcttgcccc gcccagctcc
     3241 tgcaaaagag ccgtctcgct atcatcaaga ccgtcgaaaa aatcccgcca gctcgcatct
     3301 accatatgcg gatcggccag aaactgcccg taaagatgcg caatatactc gctgttcgga
     3361 ccggacagga acgtacggtt ttcagatgat tgtgtcacaa atttaggcct cctagccgct
     3421 tagaaattct atcaatatga gccgaatatt ctactacaca gaaacagaca agaaaactac
     3481 agcgttaacg ccgtgcgaag ttgtgaaatc gcgctatctt cagccgcact gatattcatc
     3541 ggatgatctg catcgtcttg ggtcaaagac gacagattct tggtaacttt tgccaagata
     3601 ctgccaaaaa taccaccccc ttcatccggg tcgtcaaact caccaaattc tccataggcc
     3661 tgcgctacag tcgtcgcaat ctccatcaac gccgctctat aatttttaag ttcttcgtct
     3721 gaaacacgcg acttcaacac cttaactgcc tgctcggcct cttcaggaac atgccaactt
     3781 tgtgccgccc actgcgacca ttgttctcgc atttcgacag tttcacgcat aatctctgca
     3841 gtaaaaggtt tagaaatatg cagaccagca atagccatga tgcattcttc caatctatcc
     3901 atttcctgtt tatcatcacg ctcaccaccc tcatcatcgg catagctgac aaacacgccg
     3961 accttataag gcaagcacac cagcaacgcg cgatccgtat ccgaaaaatc tgaaaggcta
     4021 ctcataatga attccctata tgctgtaatc taccttcaaa gcgctggcca aacggtctaa
     4081 tgccctgcgc tcatccgtac tgatattcaa aaactgctca aaagatttaa ccggtttttt
     4141 cttaagccga gctttaagga cagtcaaagc ataggccaga aaaagcttaa acttctcagc
     4201 gccggaaggc ttgtcatgct cacgaaacgc cacagcaacc gcttccccca actccatcag
     4261 acgcatcgca taaactttca catccttctc atcaacatga gcactcaaaa cctgtaaagc
     4321 ctcggcacat tctcccggaa ttttttcaag atcaccagac cagttcaccc actcatcttt
     4381 gcgttttaag gtctcacaca taatatattg caaaagctcg gaaccaaaaa cctgctggga
     4441 aaaaccatca atcaaattcg ccagcgccgt cagctccttc tgatgcgcct cttccccacc
     4501 ttcgtcatcg ctgtaactca cccaaagccc ggcccgatag ggcagactga caatcaaggt
     4561 gcgctgttgc gcgctcaact gatccaaaac gctcataata aaaaggctcc ccggctccgc
     4621 cgtagcttat ttcttaagtc tacaacgaaa cctgaggagc atcaaagagg gaaatacggt
     4681 ttttggaaaa gcccggccat ggta
//
LOCUS       DAOSWR010000160         4697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12360_length_4697_cov_5.513356, whole genome shotgun sequence.
ACCESSION   DAOSWR010000160 DAOSWR010000000
VERSION     DAOSWR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_4697_cov_5.513356"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK513_09720"
     CDS             complement(<1..298)
                     /locus_tag="PK513_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HOO82768.1"
                     /translation="MSQLEFEKPILELEAKIAELRNVSSDSAVNIADEVKRMQDKSSK
                     LLQQAYAKLNPAQKVQVARHQNRPHFKDYLDALITDFTPLAGDRNFADDHALIGG"
     gene            complement(440..1432)
                     /locus_tag="PK513_09725"
     CDS             complement(440..1432)
                     /locus_tag="PK513_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOO82769.1"
                     /translation="MARPGRPAMPKPNLHPSVDAFLDMLTTERGAALNTRQAYWRDLA
                     DVSSYLRNSKNSDIDRATTDQLKAYLKDLSEKTHIKGANKSKIAVRTVARRLSALRQF
                     YRFMVSEGTRSDDPTSTIESPKQGRTLPKTLSEAEVTQLITTAAEPGTPESVRLVCLL
                     EMLYATGLRVSELVGLPMSAIGEDSEFLTVAGKAGRERMVPLSDPAQEALKNYMDTRK
                     RFIGTESQANQEQWLFPSRTSDSGHLTRQRFAQLLKDLARNAEVDEDRVSPHILRHAF
                     ATHLLSRGADLRSVQKMLGHADIATTQIYTHIVGENLKKTVKDKHPLSKKAGAK"
     gene            1743..1970
                     /locus_tag="PK513_09730"
     CDS             1743..1970
                     /locus_tag="PK513_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82770.1"
                     /translation="MMRKHYNPVAGTSMMTGSCSIVTGTKAKKMNPPKISKANKTPSE
                     SKNFDIVWDFSVNVEIWNDNKGLLWALQCKK"
     gene            1988..2557
                     /locus_tag="PK513_09735"
     CDS             1988..2557
                     /locus_tag="PK513_09735"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOO82771.1"
                     /translation="MAVEENLKSGILSSLTKPIALVGMMGAGKSHVGRELAEILGLKI
                     YDSDKVIEDKAGQSVADIFKNFGEKKFRGAEHNTIIELLQRGPCIIATGGGALSNPET
                     LEVLKEGSIMIWLDADFETLWERVQKSQTRPLLQTDNPEKKLKTLMQDRHPLYAQSHI
                     HFKMNAGSQQRAHEALIKALYEYLNIDKV"
     gene            2582..3754
                     /gene="aroB"
                     /locus_tag="PK513_09740"
     CDS             2582..3754
                     /gene="aroB"
                     /locus_tag="PK513_09740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOO82772.1"
                     /translation="METKIVTINLDARAYDIFIGHSLLFRIGDFIPQNLEGRNVFIVT
                     DKNVEPYAARVRSLIADSGTGICDLKVLPAGEKTKSFKHAQEVCTWMLAGGTNRDSLV
                     LAIGGGVIGDLTGFCASIVMRGVPYVQVPTTLLSQVDSSVGGKTGINTAEGKNLIGSF
                     YQPTAVIADIETLETLPRRELLAGYAEVVKYGLIEDAGFFTWLEANGERVCNLDQDAV
                     AHVLEVSCKAKARVVQADELEHGRRALLNLGHTFGHALESEAGYNGALLHGEAVSIGI
                     VMAFDLSYRMGMCSQADVERVEQHLSAMGLPTRAAAIEDLSTSVDALVVAMSHDKKIK
                     DGKLTFVLVSGIGEAFLSDDVTLDLVRDVLRESLGGETKITQKGIKERWKSAFSSL"
     gene            3727..>4697
                     /locus_tag="PK513_09745"
     CDS             3727..>4697
                     /locus_tag="PK513_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOO82773.1"
                     /translation="MEIGILLSVTAIIVLMLLSAFFSGAETALTAASRVRLLSKESDG
                     DVRAALVNKIRDQKDRMIGALLLGNNMVNILASALATSVLIKIFGETGVVYATLIMTM
                     LVLIFAEVLPKTYALHHAEGMSMRIAPIIRIVIMVFAPITETVTWIVRNVLRLFGIDI
                     SKVAAGSHLELLRGAIEMHQGPEQETQEQRAMLRSILDLADVDLEDIMTHRKNVTMID
                     ADQPMDVIIKEVLDSPFSRLPVWKDNTDNIIGVIHVKLLLCEMHERHGSVESITLENI
                     MMEPRFVPETTNLYEQLQAFRERCEHFAVVVDEYGAMMGIVTLEDIL"
BASE COUNT         1208 a         1089 c         1159 g         1241 t
ORIGIN      
        1 gtccccctat cagggcatga tcatcggcaa agttacgatc accggccagt ggcgtaaaat
       61 ccgtaatcaa cgcatcgaga tagtccttaa aatgcggacg gttttgatgc cgggcaactt
      121 gcactttttg cgccggatta agcttggcat aggcctgctg aagcaacttc gaggatttat
      181 cctgcatccg cttaacttca tcggcaatat ttactgcgct atcgctgctg acattgcgca
      241 attccgcaat ttttgcttct agttccaaaa tgggtttttc aaattcaagc tgactcatat
      301 gagtattcca tgtttggtgc gcacgtaatg atgcgctcat ttcattattg tgggacttga
      361 tagcaaatca ggcgcccaag ttcaatgaag agtgttcagt gaacatcggt cgcctgatgg
      421 aaatttggtt agaaatttct tacttcgcgc cggctttttt cgacagagga tgcttgtctt
      481 taacggtctt tttaaggttt tcaccaacaa tatgcgtgta aatctgagta gtagcaatat
      541 ccgcatgccc cagcattttc tgcacactgc gcaaatcagc gccgcggctc agcaggtgcg
      601 tcgcaaacgc gtgacgcaaa atgtgcgggc taacacggtc ttcgtcaact tcagcattac
      661 gcgccagatc tttcaaaagc tgtgcaaaac gctgacgggt caggtgaccg ctgtcagatg
      721 tgcgtgatgg gaacaaccac tgttcctgat tggcttgact ttccgttcca atgaaacgct
      781 tgcgtgtgtc catgtaattt tttaacgctt cttgcgcagg atcggacaaa ggcaccatgc
      841 gctcgcgccc ggctttcccg gcgacagtca aaaactcact gtcttcgcca attgccgaca
      901 tcggcaagcc cacaagctcg gaaacacgca agcctgtagc ataaagcatc tccagcagac
      961 aaactagacg aacactctcc ggagtacccg gctcagccgc agtcgtgatc aactgtgtca
     1021 cctcagcttc gctgagtgtt tttggcaaag tgcgtccctg tttaggactc tcaattgttg
     1081 aagtgggatc atcgctgcgt gtcccttcgg acaccataaa gcggtagaat tgacgcaggg
     1141 ccgacaagcg ccgcgctacg gtacgcacgg cgatcttgga tttgtttgcg cctttaatgt
     1201 gcgtcttctc actcagatct ttaagatacg ctttcagttg atcggtcgtg gctctgtcga
     1261 tgtcgctgtt tttactgtta cgcaaatacg agctgacatc agcgaggtca cgccagtaag
     1321 cttgacgggt gttaagcgcc gcgccgcgtt ccgtcgttaa catatctaaa aatgcatcca
     1381 cagaagggtg gaggtttggt ttgggcattg ctggacggcc tggacgtgcc ataatatttc
     1441 tcctttaaat tctattgtct taaagccttg gtaggcatca agaacatctt tatcccacac
     1501 agtacgtacc tctagaggcc tcacgcaacg atctggtttt tccttaaatt caaccttagt
     1561 atgcgtattt tgcaaaactt catagcagct ttttaaagcc agacccacat taaaatggag
     1621 tttagaagtt aggcattaca taatacacaa gccacttgag cgtcaagcat tttcataaat
     1681 tatttctgtt ttgtcaatgc ggtgactaat attgacataa ttatcattaa taaaggtatt
     1741 ttatgatgcg aaaacactat aatccggtag ctggaacttc gatgatgact ggttcctgtt
     1801 cgatagtgac aggtacaaaa gccaaaaaaa tgaatccccc gaaaataagc aaagcaaata
     1861 aaacgccaag tgagagtaaa aattttgaca tcgtttggga tttctccgtt aatgttgaaa
     1921 tttggaacga caataagggc ttactctggg cgttgcaatg caaaaaatga aaaatggact
     1981 atagacgttg gctgttgagg aaaatttaaa gtctggcatt ctttcaagtc tcacaaagcc
     2041 catagcattg gttggcatga tgggagcagg caaaagccac gttggccgag aacttgctga
     2101 aatcctgggg ctaaagattt acgattccga taaagtgatt gaagacaagg caggtcaaag
     2161 cgttgctgat atattcaaga atttcggtga aaaaaaattc agaggcgccg agcacaatac
     2221 gatcattgaa ttactgcagc gcggcccatg cataatagcc acaggcggcg gcgcactgag
     2281 caatcccgaa acgctcgaag ttttgaaaga gggcagtatc atgatttggc tggacgcgga
     2341 ttttgaaacg ctgtgggagc gcgtacaaaa gtcgcaaaca cgtccgttgt tacaaacaga
     2401 taatcccgag aaaaaactga aaaccttgat gcaagaccgc catccgctct atgcgcagtc
     2461 tcacattcat ttcaaaatga atgccgggag ccagcaacgg gcccatgagg cgttgataaa
     2521 agcattgtat gaatatctga atatcgataa ggtttgaggc tgttttatac gcgaagaagg
     2581 catggaaaca aaaatcgtta cgatcaatct ggatgcaaga gcctatgata ttttcattgg
     2641 ccattcgctg ctgttcagaa taggagattt tattcctcag aaccttgaag gccgtaatgt
     2701 ctttatcgtt accgataaaa atgttgagcc ttatgcggcg cgtgtgcgca gtctcatcgc
     2761 tgacagcggc acgggaatct gtgatcttaa ggttttgccc gctggcgaaa aaactaaatc
     2821 tttcaaacat gctcaggagg tttgcacctg gatgctcgct ggtggcacca atcgtgattc
     2881 gctggtattg gccattggcg gcggcgtaat tggagatctc acaggctttt gcgcctccat
     2941 cgttatgcgc ggcgtaccct atgtgcaggt ccccacaaca cttctttcac aggtcgatag
     3001 ttccgttggc ggtaaaaccg gcattaatac cgccgaaggc aaaaatctga taggctcatt
     3061 ttaccagccc accgccgtaa ttgccgatat tgaaacgctt gaaactttgc cacgccgcga
     3121 gttgctggcc gggtatgcag aagttgttaa atacggtctt attgaagatg caggattttt
     3181 cacctggttg gaggcgaatg gcgagcgtgt ttgcaatctt gatcaggatg ctgtcgccca
     3241 tgttctcgag gtcagctgca aagccaaagc cagagtagtg caagccgatg aattagagca
     3301 tggacgccgt gctttactca atttgggcca caccttcggt catgctttag aatctgaagc
     3361 tggttacaat ggtgcgcttc tgcacggtga agccgtttca ataggtattg tgatggcttt
     3421 tgacctgtct tatcggatgg ggatgtgttc acaggccgat gttgaacgtg ttgaacagca
     3481 tctctccgcc atggggctgc caacacgcgc tgccgcgatc gaagatttaa gcaccagtgt
     3541 tgatgcattg gttgtcgcca tgagtcatga taagaaaatt aaggacggca agctgacttt
     3601 cgttttagtt agtggcatcg gcgaggcgtt tttaagcgac gatgttacgc tggatttagt
     3661 gcgcgatgtc ttgcgcgaat ccctcggcgg agaaaccaaa atcacacaga aaggtattaa
     3721 agaaagatgg aaatcggcat tctcctctct gtaactgcaa tcattgtttt gatgttgctt
     3781 tcagcgtttt tttctggtgc agaaactgct ctgactgcgg cttctagggt tcggctgctc
     3841 agcaaggaaa gtgacggtga cgtccgcgcg gcgctggtca ataaaatcag agatcaaaaa
     3901 gatcgcatga tcggtgcgtt gctgctcggc aacaatatgg tcaacatcct tgcttccgca
     3961 ttggccacca gcgtcttgat caagattttt ggcgaaaccg gcgttgtcta tgcaacgctg
     4021 atcatgacca tgcttgtgct gatctttgct gaggtcttgc ccaaaaccta cgccctccat
     4081 catgcagagg gcatgtcgat gcgcattgcg cccataatcc gtatcgtgat tatggtcttc
     4141 gcgcccataa cagagacagt cacctggatt gtacgcaatg tactgcgcct gttcgggatc
     4201 gacatttcca aggttgccgc tggatcgcat ctggaacttt tacgtggtgc gattgagatg
     4261 catcagggcc cagagcaaga aacacaggaa cagcgcgcta tgttgcgctc gatcctcgac
     4321 ctagccgatg tcgacctcga ggacattatg actcaccgta aaaatgtcac tatgattgat
     4381 gctgaccagc ctatggacgt gatcatcaaa gaggtgctcg acagcccgtt tagccgcctg
     4441 ccggtctgga aggacaatac cgacaacatt attggtgtca tccatgtcaa gttgcttctg
     4501 tgtgaaatgc acgaacgtca cggcagcgtg gaatcaatca cattggaaaa cattatgatg
     4561 gagccgcgct ttgtccccga aaccacaaat ctctatgaac agcttcaggc ctttcgcgag
     4621 cgctgcgaac acttcgcagt tgtggttgat gagtatggtg ccatgatggg tatcgtaacg
     4681 cttgaagaca ttcttga
//
LOCUS       DAOSWR010000161         4564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12865_length_4564_cov_6.037037, whole genome shotgun sequence.
ACCESSION   DAOSWR010000161 DAOSWR010000000
VERSION     DAOSWR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12865_length_4564_cov_6.037037"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK513_09750"
     CDS             <1..255
                     /locus_tag="PK513_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOO82774.1"
                     /translation="VDILRMLLKIQAAQHDVATKLLSSTEDLEAIAMEDDADVPALKG
                     WRYEVFGREALAVKKGALAVGLKGSKIVKYKVDAQTDLWD"
     gene            288..830
                     /locus_tag="PK513_09755"
     CDS             288..830
                     /locus_tag="PK513_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO82775.1"
                     /translation="MKRLLLLRHAQALPANGGQDFDRALSPKGLEDARALGQALRQRG
                     LIPDYILCSSAKRTQQTCKESLFQTDEHKIHSSFSKDIYSASRGDLFHLIQNVRDNPA
                     TLMLIGHNPAIYELAVMLAAAGNDAVLARLSNGYQPASLSIIEAPIDDWAALDPDKCQ
                     IVDLLDPLDYNAPARPTRWT"
     gene            complement(853..1557)
                     /locus_tag="PK513_09760"
     CDS             complement(853..1557)
                     /locus_tag="PK513_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOO82776.1"
                     /translation="MVKNGAAQQIPLDLALRPAHGMEDFLIGSSNAEAIAWIDRWPDW
                     PAPLLVISGPAASGKTHLAAVWGEKTGATLINPDMLARHSAEDLAAKGERLVIDGIDL
                     WIGDLEAETTLFHLYNIIREERRSLLVTSRMNPTQSDFVIADLASRFRAAPLATIKAP
                     DDLLLGSVLIKLFSDRQLLVGNEVISYILPRMERSFLAARDIVENADKMAYARKRGVS
                     IPLIRQVLTDLQSDLS"
     gene            complement(1558..2685)
                     /locus_tag="PK513_09765"
     CDS             complement(1558..2685)
                     /locus_tag="PK513_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO82777.1"
                     /translation="MNPRNHALFWSLAFAAFLGLVFIFKAVLLPFVLGLAIAYFLNPL
                     VGALGRIKLGRGLAAMVILILFFIVVGTFLALLAPVLYKQSLQLIEDLPSYIDMVLVY
                     LEPHMQRVSEVLGQENGADLKALLSKHSGSVAQVTKQLLQGMLAGGQAVLDMISVLVF
                     TPIIAYFMMKEWMHITAWVEDLLPQQQKNTILDLLKQIDVKISGFVRGQISVAVILGL
                     SYAIALTTAGLKYGFLIGLASGLLSIIPMVGSAFGLVISVAVAWFQTGDLTFVAIIAA
                     IFLIGQIIEGNILSPKIVGESVGMHPLWVFFALLAGGSLFGILGMLLAVPVAAVAGVL
                     LAFAITQYKTSPYYGQSKEPEKQPAKKPKKSSKKSSKKKEA"
     gene            complement(2698..4020)
                     /locus_tag="PK513_09770"
     CDS             complement(2698..4020)
                     /locus_tag="PK513_09770"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HOO82778.1"
                     /translation="MVILYKTFAKARTALSVLGTCFFMLLTVPSTAQAFVESLFAVDN
                     VTVDITAENALKAREQAFEEAQVKAFDILAARMLSENELKTFERPPVMSISTMIKDYE
                     VSNEKLSSKRYIGTYKFRFEDRSVKRYFSGQGAQYTDVVSKPVLILPFLETGQGTILW
                     SYQNVWMQAWSTVPDLSSGLVPLVVPLGDLGDVSDIGDDEALTYSRRALDNMLRRYDA
                     SEAVIAIAKAEGTALNVQLYRTDRTQPEYVHQIFERALPGQDPNQIYIRAVQSVKQVL
                     RSDWKKKTVVDARQHGTIEARVSFASLQEWSDLQRRLQRVNGVSNIALKALSLKEACV
                     EITFDGTIERLKLALEQADLYLNERRMQNNTGSMNTDYSQGMHGGFLDGNDKKVQTVY
                     ELTTRHMPQAAPYQEPSYQTPLRPNTPYPRPDNNAGQNQGQPYYQSQF"
     gene            4116..>4564
                     /locus_tag="PK513_09775"
     CDS             4116..>4564
                     /locus_tag="PK513_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOO82779.1"
                     /translation="MSESAYKQAGVDIEAAAKLVENIKPHIAKTKRSGVMSGIGGFGA
                     LFDLKDTGYTDPVLVSGTDGVGTKIKIAIDSGVHDTIGIDAVAMCVNDIVVQGAEPLF
                     FLDYYATGRLDLDIAADVIKGIADGCEQAGAALVGGETAEMPGMYSEG"
BASE COUNT         1147 a         1106 c         1208 g         1103 t
ORIGIN      
        1 gttgatattt tgcgcatgct gctcaaaatt caggcggcac agcatgatgt tgctacaaag
       61 ttgctatctt ctactgaaga cctagaggct atcgcaatgg aggatgacgc agacgttccg
      121 gcgctaaaag gctggcgtta cgaggtgttt gggcgcgaag ctctggcggt taaaaaagga
      181 gcgttggcag tggggctcaa aggctctaag attgttaaat ataaggtcga cgcgcaaacg
      241 gatttgtggg attagagtca ggacctgaaa taaaggattg aaacgttttg aaacgtctgc
      301 ttttacttcg ccatgctcaa gcgctgcccg ccaatggcgg gcaagacttt gatcgcgcct
      361 taagtccgaa agggcttgaa gatgcgcgcg ctttagggca ggcgcttcgt caacgcggat
      421 tgatcccgga ttatatttta tgctcttcgg ccaaacgtac gcagcaaacc tgcaaagagt
      481 cgttatttca aaccgatgag cacaaaattc acagcagctt tagcaaagac atttatagcg
      541 ccagccgcgg agatttgttt cacctaatcc aaaatgtgag ggacaatcct gcaaccttaa
      601 tgcttattgg gcacaatccg gcaatctatg agttggctgt tatgctcgct gctgctggta
      661 atgacgcggt gctggcgcgg cttagtaacg ggtatcaacc tgcctcttta agcattattg
      721 aagcgccgat tgatgattgg gcggcgcttg acccggataa atgccagatt gtcgatttgc
      781 ttgatccgct ggattataac gccccggcgc ggccgacacg ctggacatag gtttttgggc
      841 cttgcgttta cgtcacgaca agtcgctttg cagatccgtc agaacctgac gaatcaaggg
      901 aatggatacg cctctttttc gtgcatatgc cattttgtct gcattttcta caatgtctct
      961 tgccgcaaga aatgagcgtt ccatgcgcgg caggatatag ctaatcacct cgttgccgac
     1021 aagcaattga cgatcgctaa ataattttat gagcacagag ccgagcaaca gatcatccgg
     1081 cgctttaatg gtggcgagcg gcgcagcgcg gaaacgcgag gccaaatcgg caatcacaaa
     1141 atcgctttgg gtcgggttca tgcggctggt gacaagtagg ctgcggcgtt cctcgcggat
     1201 aatgttataa aggtggaaca gggttgtttc agcttccagg tcgccgatcc acaggtcaat
     1261 gccgtctata acaagacgct cccccttcgc ggcaagatct tcagcgctat gccgagcgag
     1321 catatcggga ttaatcagcg ttgcaccggt tttttcgccc cacacagcag cgaggtgtgt
     1381 tttcccgctt gccgccggac cgctaatcac cagcaaaggc gctggccagt caggccagcg
     1441 gtcgatccag gctatggcct cagcattgct ggagccgatt agaaaatcct ccatgccgtg
     1501 cgcagggcgc aaggccagat cgagcggtat ttgctgagcc gcgccgttct tgaccattta
     1561 tgcctctttc tttttggagg actttttaga agattttttg ggcttttttg cgggttgttt
     1621 ttccggctct ttgctctggc cgtaataggg gctggttttg tattgcgtga tggcgaaagc
     1681 cagcaatacg cctgctaccg cggcaaccgg cacagccagc aacataccca aaatgccgaa
     1741 caggctgccg cctgccagca atgcaaaaaa gacccaaagc gggtgcatgc cgacgctttc
     1801 gccaacaatt tttgggctaa gaatattgcc ttcaataatc tgcccaatca ggaatattgc
     1861 ggcgatgatg gcaacaaagg ttaggtcccc ggtctggaac catgcgactg caacactgat
     1921 tacgaggcca aaagcagagc caaccatagg aattattgag agcaaaccag aggcaaggcc
     1981 aataagaaaa ccatatttga ggccagcagt cgttagggcg attgcgtagg agagtcctaa
     2041 aattaccgca acgctgattt gtccgcgtac aaacccggat attttgacat caatttgttt
     2101 gagcaaatcg agaattgtat ttttttgctg ctgcggcaag agatcctcca cccaagcggt
     2161 tatatgcatc cattctttca tcatgaaata ggcaatgatc ggggtaaaca caagcacaga
     2221 gatcatgtcg aggaccgcct ggccaccggc gagcattcct tgtaagagtt gctttgtaac
     2281 ctgtgcgacg ctaccgctat gtttactaag cagggctttg aggtcggcac cgttttcctg
     2341 ccccaacact tcagaaacac gctgcatatg cggctctaga tagacaagca ccatatcaat
     2401 gtagctaggt aaatcttcta tcaattgcag gctttgtttg taaagaacag gggcaagcaa
     2461 cgctaaaaag gtgccaacga caatgaaaaa cagaatcagg ataaccatcg ctgcgaggcc
     2521 tcggcccagc ttgatacggc ccaaggcacc cacaagcggg ttcagaaaat aggcgatggc
     2581 aagaccgagc acaaaaggta aaagcaccgc tttaaaaata aagacaaggc cgagaaatgc
     2641 ggcaaaagcc aaagaccaaa acagggcatg attgcgcggg ttcattttac gaccttttta
     2701 aaattgcgat tgatagtacg gctggccctg attttgtcca gcgttattat cggggcgcgg
     2761 ataaggggta ttggggcgta gcggcgtttg ataggagggt tcttgataag gcgcagcttg
     2821 cggcatgtgt cgagtcgtga gttcgtatac cgtttgcacc tttttatcat taccgtctaa
     2881 aaacccaccg tgcatgcctt gcgaatagtc tgtgttcatg ctcccggtgt tattttgcat
     2941 gcgccgctca tttagatata gatcagcttg ttccaaagcc agctttagac gttctatcgt
     3001 tccatcaaat gtaatctcga cacaggcttc tttgagggaa agcgccttca gggcaatatt
     3061 ggaaacccca ttgacacgct gcagcctgcg ttgaagatcc gaccattcct gcagtgaggc
     3121 aaagctcaca cgcgcttcaa tcgtgccgtg ctgacgggca tcgaccactg tctttttctt
     3181 ccaatcactg cgcaggactt gtttaacact ctgcacggcg cgaatatata tctggtttgg
     3241 atcctgacct ggtaaagccc gttcgaaaat ctggtgcaca tattcaggct gcgtacgatc
     3301 ggtgcgataa agctgaacat tcagcgctgt gccttcagcc ttcgcaatgg cgatgacagc
     3361 ttcacttgcg tcataacgcc ggagcatatt atccagcgcg cgccgcgaat aagtcagcgc
     3421 ttcatcatcg ccaatatcgg atacgtcgcc gagatcgccg agaggcacga cgagaggcac
     3481 aagcccgctg ctcaaatccg gcacagtaga ccatgcctgc atccaaacat tttgataaga
     3541 ccagagaatt gtgccctgcc cggtttcgag aaatggtaaa atcagcactg gtttgctcac
     3601 cacgtcagta tattgcgcgc cctggccgga gaaatagcgc tttacgctac gatcttcgaa
     3661 gcgaaattta taggtcccga tatagcgctt ggaggacagt ttttcgttgc tgacctcgta
     3721 atctttaatc atggtcgata tgctcataac cggcgggcgc tcgaatgtct taagctcgtt
     3781 ttccgagagc atgcgtgcgg ccagtatatc gaaggcctta acctgtgcct cttcgaaggc
     3841 ttgctcgcgg gctttcaaag cgttctcagc cgtaatatcg accgtcacat tgtcaacggc
     3901 aaacaaggat tcaacaaagg cctgcgcagt ggaaggcact gtgagcagca taaaaaaaca
     3961 tgtgccaagt acgcttaagg ctgtacgagc cttcgcaaaa gtcttataga gtatgaccat
     4021 gggcaagccc cttgtttatt ttgggattct cacgtgagag tgccttttgt tataacgggc
     4081 tgcaagaatt attggaagta ttaagagata aagtcatgag tgaaagcgca tataagcagg
     4141 ccggggtgga tattgaagca gcagccaagc tggttgaaaa catcaagccg catatcgcca
     4201 agaccaagag atccggggtg atgagcggca ttggcggttt tggcgcgctg ttcgatttga
     4261 aagatacggg ctataccgac cctgttttgg tttcgggcac agatggcgtc gggacgaaga
     4321 tcaaaatcgc gattgattcg ggtgttcacg acacaatcgg gattgatgcg gtggcaatgt
     4381 gcgtgaatga tattgtcgtg cagggcgcag agccgctgtt tttcctcgat tattacgcca
     4441 ccggccgatt ggatttagac atcgccgccg atgtgatcaa aggcatcgcc gatggttgtg
     4501 aacaagccgg cgcggcgctg gtcggcgggg aaaccgctga aatgcctggc atgtacagcg
     4561 aagg
//
LOCUS       DAOSWR010000162         4527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_12993_length_4527_cov_4.559258, whole genome shotgun sequence.
ACCESSION   DAOSWR010000162 DAOSWR010000000
VERSION     DAOSWR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12993_length_4527_cov_4.559258"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PK513_09780"
     CDS             <1..1152
                     /locus_tag="PK513_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO82780.1"
                     /translation="MFLAGAGVPTQRAVLMTGVVFAAILLDRSPISMRLVAFAAIVIL
                     LIAPESLLSASFQMSFGAVAGLVAFYSAIRPFWSALYRKAGWIRRAGLYVLGVSVTSV
                     VATLATAGFALYHFQQLAVYGLPANILAMPILAFVVMPFAVLHFLLMPFGMEGIALSV
                     MGWGIGLILDVAHFVSGLDHAVLHGRVWGGFALALFSAGFVMLVLLKGRLRIIAAIAF
                     VLCIIEILYVEQPDILISSNSKLMAIRQDNGSLLYSDLQKERFVREQWAQGFGLQKQK
                     AQKWPKEGQNGNLYCAEAGCRLERAGSRVAFLYDAQGFEEECLWADVIIAPDPLPKCE
                     GARVIDRFTTWRGGAHAITLREGDAPEIVAVEDRRGQRPWVARFQQEWL"
     gene            complement(1219..1890)
                     /gene="lexA"
                     /locus_tag="PK513_09785"
     CDS             complement(1219..1890)
                     /gene="lexA"
                     /locus_tag="PK513_09785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOO82781.1"
                     /translation="MLTKKQRDLLLFIHQRLSVDDVPPSFEEMKEALGLKSKSGIHRL
                     ISGLEERGYIERMPHRARALEIKKLPDGIKNTGRETHDATNAITQMSTANQEFDQVPL
                     YGKIAAGTPIAAIRNEGEFLQIPSGFAGNGELYALTIEGDSMINAGINDSDIVIIQRC
                     DTAENGAIIVALIDDEEATLKRLRRAGGKIVLEPENDAYEPRILEPERVKVQGRLVSL
                     MRSYH"
     gene            complement(1993..2781)
                     /gene="trpC"
                     /locus_tag="PK513_09790"
     CDS             complement(1993..2781)
                     /gene="trpC"
                     /locus_tag="PK513_09790"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOO82782.1"
                     /translation="MSVLHEICKKKRAHVEKQKNAHPLNEIKNRIADQSPPRGFIDRI
                     KTVNAAGIALITEVKKASPSKGLIRADFDPASIAKIYEDNGAACVSVLTDEPYFQGTD
                     AHFISARTAIELPMIRKDFMIDPYQIYESRALGADCILLIMAALDDSLAAELYGLATD
                     LGMDVLVEIHDAEELERALTLNPAMIGINNRNLKTLSVDVQTSFNLVSDIPENCVKIA
                     ESGLSDHNTLTKLKTAGFSGFLVGESLMRQDDIRSAVKKLRTAI"
     gene            complement(2785..3783)
                     /gene="trpD"
                     /locus_tag="PK513_09795"
     CDS             complement(2785..3783)
                     /gene="trpD"
                     /locus_tag="PK513_09795"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOO82783.1"
                     /translation="MDFYDGKTPLSENQMIEAMNQIMSGEVSDKALSTFLVNMADRGE
                     TVEELTGAARVMREKAVHIKAPYGSVDCCGTGGDKSGTYNISTAVALVAASCGVCIAK
                     HGNRAASSKSGAADVLEVLGVNLDISQERLEEALETIHFGFLMAPNHHKAMKHVMPVR
                     KVLGRRTIFNLLGPLSNPADTRHQLLGVFDKRWVSPIAETLQKLGTRRAWVVHGQDGL
                     DEITTTGPTHIAMLDAEGHIEETTLTPEDFGLGTAQPEKLLGGSAKENAQALRAVLEG
                     QKCAYRDIVLANTAAVLVIAEKTTDLKSGVRMAVDAIDSGLAMQTLKDYIAFSRGT"
     gene            complement(3797..4363)
                     /locus_tag="PK513_09800"
     CDS             complement(3797..4363)
                     /locus_tag="PK513_09800"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOO82784.1"
                     /translation="MLTLIDNYDSFTYNLVQYLGDLGVETQVHRNDQISVDEVLAKKP
                     AGLMISPGPSDPDHAGICLDLVKAAAEKEIPLIGVCLGHQTIGQAFGGKIVKTSPLHG
                     KISQITHEEKSLFAGLPSPIEVTRYHSLIIERESLPECLEITAKTEDGIIMGVMHKTL
                     PINGVQFHPESIATEHGHAMLKNFIALL"
BASE COUNT          901 a          954 c         1360 g         1312 t
ORIGIN      
        1 atgtttttgg ccggagccgg cgtgccgacc cagcgggccg tgttgatgac gggggtggta
       61 tttgcggcta ttttactcga tcgcagcccg atctcgatgc gtcttgtggc ttttgccgcg
      121 attgtcattt tgttgattgc gccggaaagt ttgctgtcgg cgagttttca gatgtcgttt
      181 ggtgcggtgg ccggattggt ggcgttttat agcgccattc gtccgttctg gtcggcttta
      241 tatcgcaagg ctggatggat acgccgtgcg gggctgtatg tgcttggtgt ttcggttacg
      301 agtgtggttg cgacgttggc gacggcgggg ttcgcgctgt atcattttca acaattggct
      361 gtctatggct tgccggctaa tattctggcg atgccgattt tggcttttgt ggtgatgccg
      421 tttgcggttt tacacttttt gttgatgccg tttgggatgg aagggattgc gctcagtgtt
      481 atgggatggg gaataggttt gattctggat gttgcgcatt ttgtttcggg tttggatcat
      541 gcggttttgc atggcagggt atggggtggt tttgcgttgg cattgtttag cgcaggcttt
      601 gttatgctgg ttttgctgaa ggggcgtttg cggatcattg cggcgatagc gtttgttttg
      661 tgtattatcg agattttata tgtagaacaa ccagatatac taatttcttc aaattctaaa
      721 ttaatggcga tccgtcagga taacgggtca ttgttgtatt cggatctgca aaaagagcgg
      781 tttgtgcggg agcagtgggc gcaaggattt ggattgcaga agcaaaaagc gcaaaaatgg
      841 ccgaaagagg ggcaaaacgg gaatctgtat tgtgcagagg ctggctgccg gttggagcgg
      901 gcaggcagcc gtgttgcttt tttgtatgat gcgcaagggt ttgaggagga atgtttgtgg
      961 gcggatgtga ttattgcgcc tgatccgttg ccgaaatgtg agggggcgcg ggttattgac
     1021 cggtttacga catggcgtgg cggagcacat gcgattacgt tgcgcgaggg cgatgcaccg
     1081 gagattgtcg cggtcgagga tcggcgtggt caaaggccat gggtggcacg gtttcagcag
     1141 gaatggctat agatcattgt attagctgtt aaaacaggat taaccagtct gttttgcagt
     1201 agcgttatgg gcgggttttc aatgatatga gcgcatcagg ctgacgagcc gcccctgaac
     1261 tttgacccgt tccggctcaa gtattcttgg ttcataggca tcgttttcgg gctctagcac
     1321 gatcttgccc ccggcgcgac gcaggcgttt aagcgtggct tcttcatcat cgatgagggc
     1381 gacgatgatg gcgccgtttt cggctgtatc gcagcgttga ataatcacaa tatcggaatc
     1441 gttgatgccg gcgttgatca tcgagtcgcc ttcaatggtc agggcgtaaa gctcgccgtt
     1501 gccggcaaat ccggagggga tttgtaaaaa ttcgccttca ttgcgaatgg cggcgatggg
     1561 cgttccggcg gcgattttgc catagagcgg gacttgatcg aattcttggt tggcggttga
     1621 catttgcgta attgcatttg tcgcatcgtg tgtttcccgt cctgtatttt ttatgccatc
     1681 ggggagtttt ttgatttcca gtgcgcgggc acggtgcggc atgcgctcga tatagccgcg
     1741 ttcttccagt ccgctgatca ggcggtggat gccggattta gatttcaacc ctaacgcctc
     1801 tttcatttct tcaaaggagg gggggacgtc atcaacactg agtcgttggt gaataaaaag
     1861 caacaaatcg cgttgttttt ttgtaagcat ggtctttttc ctcttccaat gtaatttctg
     1921 cgcgcgggca ggttcatgac aagatattac gcagatatac gctttttgtt ctactttagt
     1981 tcttgatctg tgtcaaatag ctgtgcgaag ttttttgacg gcgcttctga tgtcgtcctg
     2041 acgcatcagg ctttcaccga cgaggaaacc gctgaaacca gcggttttga gttttgtaag
     2101 tgtattgtga tcggacaggc cgctttcagc gattttgacg cagttttcag ggatatcact
     2161 taccagatta aaagaagttt gaacatcgac gcttagggtt ttgaggttac ggttattgat
     2221 gccgatcatg gcggggttaa gcgtgagcgc acgctcgagt tcttcggcat catggatttc
     2281 gacgagtacg tccatgccga gatctgtggc tagcccgtaa agctctgcgg ccagagaatc
     2341 atctagcgcg gccatgatca gcaagatgca atcagcgccc aaagcccgcg attcataaat
     2401 ctgatatgga tcaatcatga aatccttgcg gatcattggc aattcaattg cggtgcgtgc
     2461 agagatgaaa tgtgcatcag tgccttgaaa atacggttca tcggtaagca cagagacgca
     2521 ggcggctcca ttgtcctcat agattttggc gatactcgcc gggtcaaaat cggcgcggat
     2581 tagtcctttg ctgggcgagg cttttttaac ctcggtgatc agcgcgattc cggctgcatt
     2641 taccgtcttg atgcgatcga taaaaccgcg cggtggagat tgatctgcga tgcggttttt
     2701 aatttcattt aagggatgtg cgtttttttg tttttcaaca tgagcgcgct tttttttaca
     2761 aatttcgtgg aggacgctca tgtttcaggt tcccctcgaa aatgcgatat agtctttgag
     2821 agtctgcatg gccagaccgc tgtcgatggc atcgacagcc attcgaacgc cgctttttag
     2881 gtctgtggtt ttttcagcga ttaccagtac agcggcggtg ttggccagta ctatatcgcg
     2941 ataggcacat ttttgtcctt ccagaacagc acgcaaggct tgagcatttt ctttggcgct
     3001 gccccccagc agtttttccg gttgggctgt tccgaggcca aaatcttccg gcgtgagcgt
     3061 ggtttcctcg atatggcctt ctgcatccag catggcgatg tgggtggggc cggttgtggt
     3121 gatttcatca agaccgtcct gtccgtggac aacccaggcg cgcctggtgc caagtttttg
     3181 caaggtttca gcaatggggc tgacccagcg tttatcaaag actccgagga gttggtggcg
     3241 tgtatctgcg gggttggaaa gggggccgag cagattgaaa atggtgcgcc gtcccagcac
     3301 tttgcgtacg ggcatgacat gtttcatagc tttgtggtgg ttgggggcca tgagaaagcc
     3361 gaaatgaatg gtttcgaggg cctcttcgag gcgttcctgg gaaatatcga ggttaacacc
     3421 aaggacttcg aggacgtcgg cagcgccaga tttggaactg gcggcgcggt taccgtgctt
     3481 ggctatgcat acgccgcagc tggcggcgac gagggcaacg gcggtggaaa tgttgtatgt
     3541 gccggattta tcgccgccgg tgccgcagca atcaacgctg ccgtatgggg ctttgatgtg
     3601 gaccgctttt tcgcgcatga cgcgggcggc gccggtgagc tcttcgacgg tttcgccgcg
     3661 atcggccata ttgacaagga aagtagagag ggctttatcc gagacttctc cgctcatgat
     3721 ctggttcatg gcctcgatca tttggttttc gcttagcggt gttttgccat cgtaaaaatc
     3781 catgatgatg tatcctttat agcaatgcga tgaagttttt cagcatggcg tgcccatgct
     3841 cggtggctat gctttcggga tgaaactgga cgccgttgat cggcagggtt ttatgcatga
     3901 cgcccataat gatgccgtct tcggttttgg cggtgatttc gagacattcc ggcaggcttt
     3961 cgcgttcgat aatcagtgag tgatagcgcg tgacttcaat cggcgagggc aggccagcaa
     4021 agaggctttt ttcctcatga gtgatttgtg aaattttacc gtgaaggggg ctggttttga
     4081 cgatttttcc gccaaaggcc tgacctatgg tttggtggcc gaggcacacg ccgatcaggg
     4141 gtatttcttt ttctgcggcg gctttaacaa gatcaaggca aataccagca tggtcaggat
     4201 cgctcggacc gggggaaatc ataagcccgg ctggtttctt ggccagaact tcgtcaacac
     4261 tgatttgatc gttgcggtgg acttgtgttt ccacacctaa atccccgaga tattgcacga
     4321 gattgtaggt aaagctgtcg taattatcga tgagagtaag catggaggca tttatggcgt
     4381 aaacaggcct gtgaatgcaa gggctaatat tgtttgacga tggttgttaa gctttgaaaa
     4441 tatggatatt attgcgctat gaaacgctat gcaaacccgc gcggaactgt tctgttcacg
     4501 cttattgccg ttattttgat gctgatt
//
LOCUS       DAOSWR010000163         4517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13027_length_4517_cov_5.810847, whole genome shotgun sequence.
ACCESSION   DAOSWR010000163 DAOSWR010000000
VERSION     DAOSWR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13027_length_4517_cov_5.810847"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK513_09805"
     CDS             complement(<1..555)
                     /locus_tag="PK513_09805"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOO82785.1"
                     /translation="MSAPTTQIDFKNKSPEYFKTLADHCASFKGADTKRSITQMTVTL
                     ALFTAALIGLTLSVQSGNWLLYALLLIPTGGLLTRIFIFQHDCGHGSYFKTRKANDNV
                     GRALSILTWTPYSFWRRTHNMHHASSGNLDKRGYGGIETITLNEFKSLPPAKQRFYRL
                     YRNAYLLLGLGTPLFVLVVQRFLVT"
     gene            complement(664..2481)
                     /gene="typA"
                     /locus_tag="PK513_09810"
     CDS             complement(664..2481)
                     /gene="typA"
                     /locus_tag="PK513_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013695204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOO82786.1"
                     /translation="MTKNLRNIAIIAHVDHGKTTLIDSLMKQSGMFRENQNVDVCVMD
                     SGDLEKERGITILAKPTSIQWGDVRINIIDTPGHADFGGEVERVLNMADGVILLTDAA
                     EGPMPQTKFVLGKALAQGLNPIVIINKIDRPDGRPEEVVDEVFDLFVSLDANDKQLDF
                     PIMYASGRDGWCVKELDDPRENLTPLLDLVLKHVPAPSGDKDAPFAMLATLLDSDPFL
                     GRCLTGRVINGCAKVNDTVKAINLNGDLIENGRLTKLLTFDGTQRVPVSEVSVGDIIC
                     IAGLTKASVSDTIGAPALNTPVASTPIDPPTMAVMITVNDSPFAGTEGKKVTSTMIRQ
                     RLLNEAEVNVAITFKESESKDSFEIGGRGELQLGVLIETMRREGFELTVSRPRVLFKD
                     VDGQCHEPIEEVTIDVDEEYASTVVDKMNRRKSEMTDMRSSGSGKTRITFLAPSRGLI
                     GYQSQFVTDTRGTGVFNRVFHSYAPYKGDIPQRRHGALISTDQGQAVAYAIFNLQDRG
                     VMFVEHQTPVYAGMIVGEHSRDNDLEVNVLKGKKLTNVRASGSDDAVTLTPPRKMSLE
                     DMMAYINDDELLEVTPTNLRLRKLHLCPHERKKASRAAS"
     gene            2636..3070
                     /locus_tag="PK513_09815"
     CDS             2636..3070
                     /locus_tag="PK513_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOO82787.1"
                     /translation="MSEIPIVGVQQAHEWLQNGEAVIFDVRDQGEYDEEHIPGAVLNP
                     LSMFSVQNVEQICQNRKIIFQCKSGKRAQMALERYMKEGPCARGENHLFCLEGSLPGW
                     KAAGLPTEKGRKVISLERQVMSVFLENNGNTLKQNTINKSKG"
     gene            complement(3154..4335)
                     /locus_tag="PK513_09820"
     CDS             complement(3154..4335)
                     /locus_tag="PK513_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO82788.1"
                     /translation="MSFIPRNAEKILKKLAKSYPAVVVTGPRQSGKTTLTQAVFSDKP
                     YVSLEDPDMREFADTDPRGFLAQYPKGAILDEVQRCPDIFSYLQTRLDADKKMGQFIL
                     TGSQQFGLISGISQSLAGRTAKISLLPFSLQELQQAGKAPKKLEDLLFKGLYPPIYDR
                     KLEAGIWHANYIDTYVERDVRQLINVRDLSTFQRFVRMCAARTGQLLNLSDLAGDCGI
                     THNTAKAWVSVLEASYIIHLLQPHHKNFNKRLIKSPKLYFYDTGLAAWLLNIQTPEQL
                     ATHSARGQLFETWVVSELIKYRYHQALGSNLYFWRDHKGHEVDVLIEQADKLIPVEIK
                     SGQTVASDFFDGLKKFQDIAGKESGQGTLIYGGSSSQTREAFNVIGWQDIGAVEKALK
                     A"
BASE COUNT         1084 a         1010 c         1223 g         1200 t
ORIGIN      
        1 cgttactaaa aagcgttgaa cgacgagcac aaagagcggc gtaccgaggc cgagcagcag
       61 atatgcattg cggtagagtc ggtagaagcg ttgtttggcg gggggtaagg atttaaattc
      121 attcagtgtt atggtttcaa tgccgccgta gccgcgtttg tcgaggttgc cggaactggc
      181 atggtgcatg ttgtgggtac gccgccagaa gctataaggg gtccaagtca ggatgctgag
      241 ggcgcgtcca acattgtcat tggctttacg ggttttgaaa taggaaccgt ggccgcaatc
      301 atgttggaaa atgaaaatac gggtcagcag gccgccagtg gggatgagga gcagtgcata
      361 gagcagccag tttccacttt gaaccgagag ggttaagccg atgagcgctg cggtgaacag
      421 cgccagcgtg acggtcattt gcgtgatgct gcgctttgta tctgcccctt taaagcttgc
      481 gcagtgatcg gcaagggttt tgaaatattc tggagattta tttttaaagt cgatttgggt
      541 ggtgggggcc gacataggaa ctcttcattt gttttttgca attaatggat aagtgttgca
      601 tttacactat gcgctgccaa ggcatagtgg gtaattatcc aaagttctct aaaagaagaa
      661 ttgttagctt gctgcgcggc tggctttttt acgctcatgc gggcagaggt ggagtttgcg
      721 cagacgtaag tttgtcgggg tgacttccaa taattcatca tcgttgatat aggccatcat
      781 gtcttcaagg ctcattttgc gtggcggtgt taacgttacg gcatcatcgc tgccagaggc
      841 gcggacgttg gtgagttttt tgcctttgag gacgtttact tccagatcat tgtcacgact
      901 gtgttcgccg acaatcattc ctgcgtaaac aggggtttgg tgttcaacaa acattacgcc
      961 gcggtcttgc aggttgaaaa ttgcataggc gacggcctgg ccttgatctg tggagatcag
     1021 cgcgccgtgg cggcgttgcg ggatgtcgcc tttatatggc gcgtaggagt ggaatacgcg
     1081 gttaaatacg cctgtgccgc gggtatcggt cacgaactgg ctttgatagc caatcagacc
     1141 gcgtgagggg gcaaggaatg tgatgcgggt tttgccagaa ccggatgagc gcatatcggt
     1201 catttccgat ttgcggcggt tcattttatc aacgaccgtg gaagcgtatt cttcgtcaac
     1261 atcaatggtg acttcttcga tcggctcgtg gcattggccg tctacatcct tgaagagaac
     1321 acgtgggcgc gacaccgtca gttcaaagcc ttcacggcgc atggtttcta tcaaaacgcc
     1381 aagctggagt tcaccgcggc cgccgatttc gaatgaatct ttgctttcgc tttctttaaa
     1441 ggtaatggct acattgacct ctgcctcatt gagtaagcgc tgacggatca tggttgaggt
     1501 gacttttttg ccttcggtgc cggcgaaagg ggagtcgttc acggtgatca tgacggccat
     1561 tgtgggtgga tcgatggggg tggaagctac gggtgtgttc aacgcgggcg cgccgattgt
     1621 atcggataca gacgctttag ttagacctgc gatgcagatg atgtcgccga cactgacttc
     1681 gctaacggga acgcgctgcg tgccatcaaa ggtcaggagc ttcgttaaac gcccattttc
     1741 gatgagatca ccattcaaat ttatagcttt tacggtgtcg tttactttgg cgcagccgtt
     1801 aattacgcgg ccagtcaggc agcggccaag gaacggatca gaatcaagca aggtggcgag
     1861 cattgcaaag ggggcgtctt tatcgccact tggcgcagga acatgtttca ataccaaatc
     1921 gagcaggggc gtcaggtttt cacgcgggtc gtccaattct ttaacgcacc agccatcccg
     1981 gccagaggcg tacataatcg ggaagtcgag ctgtttgtca ttggcgtcta aggatacgaa
     2041 gaggtcaaag acctcgtcga caacctcttc cgggcggcca tcggggcggt caattttatt
     2101 tataatgacg atggggttca ggccttgggc cagggctttt cccagaacaa attttgtttg
     2161 gggcattggg ccttcagcgg catcggtcag taaaattaca ccgtcggcca tgtttaatac
     2221 tcgctctact tcgccgccaa aatcggcgtg gcctggtgtg tcgataatat tgatacgaac
     2281 gtcaccccat tgaatggagg ttggtttggc aagaatggta atgccgcgct ctttttcaag
     2341 atcaccggaa tccatgacgc aaacatcgac attctggttt tcgcgaaaca tgccgctttg
     2401 tttcataagg gagtcgatca gcgtggtttt gccatggtcg acatgggcga tgattgcgat
     2461 gttgcgtaag ttttttgtca tggtccgttt ttctttcttt ataacccgca aagtcctata
     2521 ggagtgcggt ccaaaaggga agtaaaataa atgcgttttt gtgtatgcag ccttgactcg
     2581 tacttatata ttattaaatg cttatacgta attttctttt ttgaggattt tgattatgtc
     2641 tgaaataccg attgtcgggg tgcaacaagc gcacgagtgg cttcaaaatg gcgaggctgt
     2701 gatttttgat gtgcgtgatc agggggaata tgacgaggag catattccgg gcgctgtttt
     2761 gaacccttta agtatgtttt cagtgcaaaa tgttgaacag atctgtcaga accgaaaaat
     2821 tatttttcag tgcaagagcg ggaaacgggc gcaaatggcg ttggaacgct atatgaaaga
     2881 ggggccttgc gcgcgtggtg agaaccattt gttttgtctg gagggtagtt tgccaggctg
     2941 gaaggcagct gggttgccga cagaaaaagg gcgcaaggtg atttcgctgg aacgtcaggt
     3001 catgagcgtg tttctagaaa ataatggcaa cacactaaaa caaaatacaa taaacaaatc
     3061 aaaaggttag agtaaagctg cttttgccgc aattcgttac ttattgtccc tttttgccgc
     3121 caactgtccc accagtgtcc cttggaattg gggttatgct ttcagagcct tctctacagc
     3181 cccgatatcc tgccagccga ttacattgaa cgcttcccgt gtttgcgagc tagaaccgcc
     3241 atagatcaga gtgccctggc cggattcttt gccagcgata tcctgaaatt tcttcaagcc
     3301 atcaaagaaa tcgctcgcca cagtctggcc agattttatt tctaccggga tcagtttatc
     3361 ggcctgctcg atcaaaacat caacctcatg ccccttgtga tcgcgccaga aatacaggtt
     3421 tgatcccaaa gcctgatgat aacggtattt gatcagctct gatacaaccc atgtttcaaa
     3481 cagctgcccg cgcgccgaat gtgttgccag ctgttcgggc gtttgaatat ttaaaagcca
     3541 ggctgcaagg cctgtatcgt aaaaatacag cttgggtgat ttaatcagac gcttgttaaa
     3601 attcttatga tggggctgca gcagatgaat aatataactg gcctccaata cagagaccca
     3661 tgccttggcg gtgttatgcg taatgccgca gtccccggca agatcggaca ggtttagaag
     3721 ctgtcccgtt ctggcagcgc acatgcggac aaaacgctgg aacgtgctta aatcacggac
     3781 attgataagc tggcgcacgt cccgctcgac ataggtatcg atatagttcg cgtgccagat
     3841 accggcctcc agcttacgat cataaatcgg tggatacaga cctttgaaca acagatcttc
     3901 cagctttttg ggcgctttac ccgcctgctg taattcctgc agactgaacg gcaaaagtga
     3961 aattttggcc gtccgtcccg ccaaactctg ggatatgccc gaaatcagtc caaattgctg
     4021 agagccggtc aggataaact gccccatctt cttatcggca tccagacggg tttgcaggta
     4081 ggaaaagata tcaggacaac gctgcacctc gtcaagaatg gcgcctttgg gatattgcgc
     4141 cagaaagccg cgcgggtctg tatcggcgaa ttcgcgcata tcgggatctt ccagagacac
     4201 atagggttta tcagaaaata cggcctgcgt gagcgttgtt ttgcctgatt gacgcggccc
     4261 ggtcacaacg acagccggat agctcttcgc cagctttttt aatattttct ctgcgttacg
     4321 aggaataaag ctcatagggg taatttactg ctattagtac aaattgtcaa ttcaatttac
     4381 aatttgtacc aaaccacttg acctgccccc tataattcag tccagttttt agttaggctc
     4441 aggacctata aacaggatcg attagggtcc ggactcacag cttaaatcca gaaacataag
     4501 agggaagcaa ggcatag
//
LOCUS       DAOSWR010000164         4506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13069_length_4506_cov_3.497192, whole genome shotgun sequence.
ACCESSION   DAOSWR010000164 DAOSWR010000000
VERSION     DAOSWR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13069_length_4506_cov_3.497192"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2677)
                     /locus_tag="PK513_09825"
     CDS             complement(<1..2677)
                     /locus_tag="PK513_09825"
                     /inference="COORDINATES: protein motif:HMM:NF023351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HOO82789.1"
                     /translation="MLIPLHWGFSASPVNAGETSSSLVFQKLDNIDPEALSPEEAKSL
                     IRELKSLAQKEEAASSSLYKSTPYPKKEREYSAWERRIAQEVIATGQMLEGDPRFDDV
                     VSTLAQRGRSILPDGSNVTQDWVRENLQDVLPAIDNKMAAAAMNAATGEIARGTGDMA
                     NGFAQSQIGSQIGLSGEGQSYDLGGNLKQTAIRSGLEGVKAAASASDIYALNHLELEY
                     SLSGDGIDEYSVLTVQPLWDSADLRHNIFAQASYANKQVEDIGADTSQRRDTVNAGLA
                     YRYITPDEQHMFGANAFFDHQWPYHHNRMSLGLDYKTSLYGVAFNKYVGLSDWRGRDD
                     GYEEKALGGEDLEVSGRLPQAPELEVFAKGYHWSQEKTPVINPDGDDIWGYQFAAEYT
                     PVNAFTIRSQATRDNEMDDMEGQITMRLNYRFGQGWDDLWKRPTYNLDSVLDRRFEKV
                     RRNNEIRVQVRQDPDVTARVTFAQGANVSVGQSLAFGTTITTGGAGGDGATVVFGNGA
                     RLDVGQNTQVRIEKDQIVLITGIIQFTSGSGGITVIAVPGGTIDLIGTDVDVRVSGGT
                     TTLRVRDGAADFTDDTGTTRVNTEELAEAQDGDGLAPQIRAEGTAIYETHTSEAHTQL
                     DLVGPQPSNPKAAPFADEAVSVAGTLATGNTLTFTVPLTGSVTVTGMPRLRFTLGGLD
                     RLADYASGSGTSTLVFTYDVVGADETLSNIVAEEIEKNGGTLTGTNGAPMVRTVSGGL
                     SGTVPDVTMPTIAGFTAVSSGGDPAGIGDIITVTLNANEDLVQSGTPSLTLNIGGTSR
                     TANFSAINAGNAEFTYTVQAGDNDADGITITAITVAANELEDASGNDLDTTFALPYNL
                     SLDVSTILLGLSSCPSGDLSAPANSGCARLFGADPT"
     gene            complement(2897..3364)
                     /locus_tag="PK513_09830"
     CDS             complement(2897..3364)
                     /locus_tag="PK513_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82790.1"
                     /translation="MSATQKLIIVFFSAFLCFGFVKPAYAESEDACAIWICLPGGFPQ
                     GCSGAYSEFRHRIKKGRPPLPELSSCTTGPNGEKVSGHYQLGYERYEPCEDGYVLRES
                     QQYNQAKCYVARCAPGRYSNDNHYCDSYQAVLRPKPNYVKMWVDGDYLGQYFY"
     gene            3387..3980
                     /locus_tag="PK513_09835"
     CDS             3387..3980
                     /locus_tag="PK513_09835"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOO82791.1"
                     /translation="MDSIKTVKQKMKRLQFFFGVTALALSVFSWPAFATVYEFQGDGN
                     VVVFEAHDYLSKAHLPKTGYAVFAPIKKEAYNRIIESASKKYKLDKNLIHAVIYVESS
                     YRPNALSPKGAQGLMQLMPGTAKKYGVKDAFDPDQNISGGSRYLRDLLDLYEGNRTLA
                     LAAYNAGENAVRKYNGVPPFEETINYIEKVETALRSF"
     gene            4149..4460
                     /locus_tag="PK513_09840"
     CDS             4149..4460
                     /locus_tag="PK513_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HOO82792.1"
                     /translation="MSKNINFNVQGVLFFLAFSLIIMLPDMAYAQTALFDSGTNFLTA
                     LQDLLTNTWARIIAIIAICGLGILWMTGRLAWGVAASIIGGIILIFGAPAIVDSISGN
                     I"
BASE COUNT         1095 a         1209 c         1059 g         1143 t
ORIGIN      
        1 cagttggatc ggccccgaac agcctcgcgc agccggaatt ggccggggca gacaaatcac
       61 cggacgggca agatgacaga cctagaagga tcgtacttac atcaaggctt aaattatatg
      121 gcagagcaaa tgtcgtatcc agatcgttac ctgacgcatc ttccagctcg ttcgcggcga
      181 cagtgatcgc ggtaatcgtg atcccgtccg cgtcattgtc ccccgcctgc accgtgtagg
      241 taaattcggc gttaccggca ttgatcgcgg agaaattcgc ggtgcgggac gtgccaccaa
      301 tattcagcgt taagctcggc gtgccgcttt gcacaaggtc ttcattcgcg tttaatgtca
      361 ccgtgattat atcgccaatt cctgccgggt cgccgccgct cgataccgcc gtaaagcctg
      421 caatcgtcgg catggtgaca tccggcaccg tgccggacaa tccgccgctg accgtccgca
      481 ccatcggcgc gccgtttgtg ccggtcaacg taccgccgtt cttctcgatt tcctcggcaa
      541 cgatattgga caatgtttca tccgcaccca caacatcata agtaaagaca agcgtggatg
      601 tgccgctgcc gctcgcataa tcggccagac gatccagacc gcccagggta aagcgtagcc
      661 tcggcattcc ggtgaccgtg acgcttcccg tcaatggcac ggtaaaggtc agtgtattgc
      721 ccgtagccag cgtgccggcc acactgaccg cttcatcggc aaaaggcgcg gctttcggat
      781 tggaaggctg cgggccgacg agatcaagct gcgtgtgcgc ttcacttgta tgtgtctcat
      841 aaatggccgt accttcggcg cggatttgcg gtgccagccc atccccatcc tgtgcttcgg
      901 ccagttcttc cgtattcacg cgcgttgtac cggtatcgtc tgtaaagtcc gctgcgccat
      961 cacgcacccg cagcgtcgtg gtgccgcccg atacgcgcac atccacgtcc gttcctatca
     1021 ggtcgattgt accgcccggc acggcaataa ccgttatgcc gccactgccg ctggtgaatt
     1081 gtataatccc ggtaatcagg acgatctggt ctttttcaat ccgcacttgc gtattttgtc
     1141 ccacatccag ccttgcgccg ttgccgaaga ctaccgtggc tccgtcgccg cccgcgccgc
     1201 cggtcgtaat cgtcgtgccg aaggccagag attgcccgac actgacattt gccccttgtg
     1261 caaaggtcac gcgcgccgtt acatcagggt cttgacgcac ctgtacccgt atttcattgt
     1321 tccggcgcac tttctcaaaa cgccgatcca gaacggaatc aagattgtaa gtcgggcgct
     1381 tccacaaatc atcccatccc tgcccgaaac gataattcag gcgcatggta atctgccctt
     1441 ccatatcgtc catttcattg tctcttgtcg cttgggaacg gatcgtaaag gcattcaccg
     1501 gcgtgtattc cgccgcaaac tgatagcccc atatatcgtc cccgtccgga ttgataacag
     1561 gtgtcttttc ctgactccaa tgatagccct tggcaaacac ttccagttcc ggggcttggg
     1621 gcaaacgccc cgaaacttcc agatcttcac ctcctaatgc tttttcttca taaccgtcat
     1681 cacgtccgcg ccagtcggac aggccgacat atttattgaa cgccacaccg tacaggctgg
     1741 ttttataatc cagccccaac gacatgcgat tatggtgata cggccactgg tgatcgaaga
     1801 aggcattggc cccgaacata tgctgttcat ctggtgtaat gtagcggtaa gcaagcccgg
     1861 cattcaccgt atcgcggcgc tggcttgtat ccgcacctat atcttcaacc tgtttgttgg
     1921 cgtaggaagc ctgcgcgaaa atgttatgac gcaaatcggc actgtcccag agcggttgga
     1981 cggttaaaac cgagtattca tcaattccgt cacccgaaag ggagtattcc agttccaggt
     2041 gattcagagc ataaatatcg cttgccgacg ctgctgcctt taccccttcc aggccgctgc
     2101 gaatggccgt ttgtttaaga ttgccgccta gatcatagct ttgcccttcc ccggataagc
     2161 caatttgcga tcctatctgt gattgggcaa aaccgttcgc catgtcgccg gttcctctgg
     2221 caatctctcc cgttgccgca ttcatcgccg ctgccgccat tttattatca atcgcgggta
     2281 aaacatcctg caaattctcc cgcacccagt cctgcgtcac gttagaacca tccggcaata
     2341 tcgagcgacc acgctgcgcc aacgtggaca ccacatcatc aaatctgggg tcgccctcca
     2401 gcatttgccc cgtggcaatg acttcctgcg cgatacgccg ctcccacgcg ctgtattcac
     2461 gctctttttt gggataaggg gtgcttttat ataatgaaga agaagccgcc tcctctttct
     2521 gcgccaaact tttcagttca cgaatcaggc ttttcgcttc ttcaggagaa agggcttcgg
     2581 gatcaatatt atcaagtttt tgaaatacca gagaggatga agtttcccct gcattgaccg
     2641 ggcttgccga aaatccccag tggagcggga tgagcaaaag gaacataagt ccagtggtaa
     2701 aatgtctaaa ctgttttccc ggcaagcgca tatcgaacaa aatctgggtt gaatacaatc
     2761 gtgagcggaa aacacttaga attccacagt ttatattata aaaatcgggg gggggggaca
     2821 aggggtaatt tagaaaaaac gccagatttt caagaacttt gcaaaaaatc caccgattta
     2881 acaaccgcaa gaggtgttag taaaaatact gccccagata atcaccatca acccacattt
     2941 ttacgtagtt gggttttggg cgcaaaacgg cttggtagct gtcgcaataa tgattatcat
     3001 tgctgtatct tcccggcgcg catcttgcaa catagcactt tgcttggttg tattgctggc
     3061 tttcccgcaa tacatagcca tcttcacaag gctcgtagcg ttcatatcct aactgataat
     3121 ggccgctgac tttctcccca ttcgggccgg tagtacatga cgatagctcc ggcaatggtg
     3181 ggcgtccctt ctttatccga tgcctaaact cactgtaagc accactacac ccctgcggaa
     3241 agccgcccgg taagcatatc caaatagcgc aggcatcctc cgattccgca tacgctggct
     3301 tcacgaagcc aaaacaaaga aaggccgaaa aaaagacaat aattagtttt tgggttgcgc
     3361 tcataactca aaatatacaa tatcagatgg attccataaa gactgttaag caaaaaatga
     3421 agcgattgca atttttcttt ggtgtaactg cgttagctct gtcggtattt tcctggcctg
     3481 cgtttgctac tgtatatgag tttcaggggg acggaaacgt ggttgttttt gaagctcacg
     3541 actatctctc taaagcccat ctacctaaaa ccggctatgc tgtcttcgca cctatcaaaa
     3601 aggaagcata caatcgtatt attgaaagtg cctctaagaa atataaactc gataaaaatc
     3661 tcattcatgc tgtgatttat gtggaatcct cctatcgccc caatgccctc tcaccaaaag
     3721 gcgcacaggg gcttatgcaa cttatgcctg gaacagcaaa aaaatatggc gttaaggatg
     3781 cgtttgatcc agaccagaat atcagcggcg gatcgcgtta tctgcgggat ttactagatc
     3841 tatatgaagg aaaccggact ctggccttgg ccgcttacaa cgcgggggaa aatgctgtca
     3901 gaaaatacaa tggtgtgccg ccatttgagg aaacaataaa ttacatagaa aaggttgaaa
     3961 ccgctttaag aagcttttaa gattcctgta ccctgtggat tgaaatctaa ttatagttga
     4021 aaatttcaac tttccgtatt ttaactcttg aactttgtaa cccaatgttg cactctcgct
     4081 atattcagca atggtgtgac aaacggcggg caataatgcc tgctgaagac atataagggt
     4141 tagaagctat gagtaaaaac ataaacttta atgtgcaggg tgtacttttt ttcctcgcat
     4201 tctccttaat cataatgttg cctgacatgg cttatgctca aacagctttg tttgactcag
     4261 gaaccaattt cctgacagcc ctacaggatt tgctcacgaa tacgtgggct agaattatag
     4321 cgattattgc catttgcggc ctcggcattt tgtggatgac tggacggctc gcgtggggcg
     4381 ttgcggcgtc aataatcggc gggattattt tgatttttgg agcgcctgct atcgtcgata
     4441 gtattagcgg aaacatttaa caactagata cgacatggaa gaggatcagg taagcattga
     4501 tcccct
//
LOCUS       DAOSWR010000165         4499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13105_length_4499_cov_5.075608, whole genome shotgun sequence.
ACCESSION   DAOSWR010000165 DAOSWR010000000
VERSION     DAOSWR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13105_length_4499_cov_5.075608"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2722)
                     /locus_tag="PK513_09845"
     CDS             complement(<1..2722)
                     /locus_tag="PK513_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82793.1"
                     /translation="MAVNTPDVQKILLGKVRDWLNTNHDALNALVPNDHAQKAALTIN
                     EIPELSALPNSTEIVSKTAQQAHAILEQFDNQALRENFANEIMPALQVIAISEAIKTD
                     LENNTISLETQAGTFQIKWNHMRNGTINPELENGFSYRTHDGKKLESLDAQNAINSAL
                     IDLTAGPLIQGEMAKVIARRLQETDRDADDKINFRDIRAVRQGFLSQTGRLTPNNFID
                     IATIPAFYKAMELVKDDVEADVFERVMKDAESDIKMAQEAFSALTETQTPAQPDAPAQ
                     QKKPPAEKPAASTQQENPPAAAKKPEITKPATEKSATTEEPKAKAKAKPKNETIVLSD
                     YLREEVQYATLLLERAFAEGKSHKYLFYNEPDKSLDPATLAQIRDTLITAVSKYGQEY
                     YDGDKYILGPDDKTRYTFSKEGYIYSYQIIDGKPTDKETIGKITDGLNLEIAEEGEFK
                     GKLVGQIKPNPIVVALINDIRKDLFAVDIDAEQEKLALEQRKLEDKQNRLIEDYFNAN
                     PALASLEDEFREALKKHDNADPFKNTAIAKKIREQSADKETFDLFLVAILTNQQSMNE
                     LGAKSQNFGQERADISAIANLFRVMSDDKLYDPNKLSELYGKADFKPDNILSLNAYTW
                     LEGYKGDHPLAVEALYLGLDEERKQAILQAFEGKTQVTLQEARVFVRRQIDLRAIKTL
                     DPGTYADAEKAGKLDTIAGQNAFIAANHATLRGVSQAMMEGRFNPDERDVKIAFKAFG
                     TLSIQDPRIRRHINRMMTLTDNTRPNAKYAQYVLTPDELETLTKDKVKELTDVGIIKS
                     PPGTEFKALASVAKEKVLQSILAQPLWNDPGRPGFEAYERLMLQYYESHINNYHAWHN
                     GDVNDQTMDTWNGRYGGPTQDQMMYLYVRNNWHHMNEIFKGDTSFFFNKSG"
     gene            complement(2970..3869)
                     /locus_tag="PK513_09850"
     CDS             complement(2970..3869)
                     /locus_tag="PK513_09850"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOO82794.1"
                     /translation="MALIPYDDRDGFIWMDGKMLPWREAKIHYLTHALHYGTQVFEGE
                     RAYDGHIFKSREHSQRLHNSAKIIYMDMPVSVDELENIKQEVLKANNLENAYIRAAAW
                     RGAEQMGIDVAGTKTHVAIAAWDWGSYFDPAIREKGISLKTSTWRKPAPNTSPTAAKT
                     ASLYNLSCMVKVEVKKAGYTDALMLDHEGYVAESTGANLFAVKNGQIHTPIADRFLNG
                     ITRQTVMSLARERNISVEERRIKPEELESFDEIFLTGTAAEVTAVGKIDDITYSVGPI
                     TRKLHEAYEDLIHGRTQDVKQSA"
     gene            3938..>4499
                     /locus_tag="PK513_09855"
     CDS             3938..>4499
                     /locus_tag="PK513_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82795.1"
                     /translation="MMMSKSSQENYPKDGGSYEAWPHVLVVDDDRRIRDLLCRYLSEQ
                     GFVVIAAADAFEATALLESFEVDVLVVDVMMPGKTGLEFTAGYRESGSVVPILLLTAL
                     GETEDRITGLEAGADDYLSKPFEPRELVLRLNAILKRTAKPVHVERSLKIGPWVFDPV
                     HEELSAGGDVVRLTSAEAQLLKALGAR"
BASE COUNT          847 a          918 c         1318 g         1416 t
ORIGIN      
        1 tgccgctttt attaaagaaa aagcttgtgt cgcctttaaa gatttcattc atgtggtgcc
       61 agttattacg cacgtaaaga tacatcatct gatcctgcgt tggcccgccg tagcggccgt
      121 tccaggtgtc catggtttgg tcgttgacgt caccattgtg ccaggcgtga tagttattaa
      181 tgtggctttc gtagtattgg agcatcaggc gctcataggc ttcgaagccc gggcggcctg
      241 ggtcattcca cagcggctgt gcaaggatgc tttgcaggac tttttctttt gcgacagagg
      301 ccagagcctt gaattccgtg cccggtgggg attttataat tccgacgtct gtgagttctt
      361 tgaccttgtc ttttgtaagt gtttccagtt catccggcgt caggacatat tgcgcgtatt
      421 ttgcgtttgg acgggtgttg tccgtcaggg tcatcatgcg gttgatgtgc ctgcggattc
      481 tcgggtcttg tatgcttagc gtaccgaagg ctttgaaggc gattttgaca tcgcgttcgt
      541 cggggttgaa gcggccctcc atcatggcct ggctgacgcc gcgcagggtt gcgtggttgg
      601 cggcgatgaa ggcgttttgc ccggctatgg tatcaagttt gcctgctttt tcggcgtcgg
      661 cgtaggtgcc tgggtccagg gttttgatgg cgcgcaggtc gatctggcgg cggacaaaaa
      721 ctctggcctc ttgtaaagtg acttgcgttt tgccttcgaa cgcttgcagg atggcttgtt
      781 ttctctcttc gtcaagcccc agatagagcg cttcgacggc aagggggtgg tcgcctttat
      841 agccttcgag ccacgtatag gcgtttaatg aaagaatatt atccggttta aagtctgctt
      901 tgccgtatag ctccgatagc ttattcgggt cataaagttt gtcgtcgctc atgactcgga
      961 agagattggc aatggcgctg atgtctgcgc gttcctggcc gaagttttgg ctttttgcgc
     1021 cgagttcgtt catggattgt tgattggtca gaatggcgac gaggaagaga tcaaaggtct
     1081 ctttatcggc gctttgctcg cggattttct ttgcgatggc cgtgtttttg aacgggtcgg
     1141 cgttgtcgtg ttttttgagg gcttcgcgga attcatcttc tagacttgcc agagccgggt
     1201 ttgcattaaa atagtcttca atgagtcggt tttgcttgtc ttcaagcttt ctttgttcga
     1261 gggcgagttt ttcctgctcg gcatctatat cgacagcgaa gaggtctttt ctgatgtcgt
     1321 tgatgagggc aacaactatc gggttgggtt tgatctgccc gacgagcttg cctttaaatt
     1381 cgccctcttc ggcaatttca agattcaggc catcggtgat ttttccgatg gtttctttgt
     1441 ctgtcggttt gccatcgatg atttggtagc tgtagatata gccttctttg ctgaatgtgt
     1501 agcgggtttt atcgtccggg ccgaggatgt atttgtcgcc gtcgtaatat tcctggccgt
     1561 atttagaaac tgcggttatt aacgtgtcgc gaatttgcgc gagtgtggct ggatcgaggg
     1621 atttatccgg ctcattgtaa aacagatatt tatgtgattt gccttcggcg aatgctcgct
     1681 caaggaggag ggtggcgtat tgcacttctt ctctaagata atcagagagg acgatggttt
     1741 cgttttttgg ctttgccttt gcctttgcct ttggctcttc tgtggtggcc gatttttctg
     1801 tagcaggctt tgtgatttct ggcttttttg ctgcagctgg agggttttct tgctgcgttg
     1861 atgctgcagg tttttcggcc ggaggttttt tttgttgggc tggcgcgtcc ggttgtgccg
     1921 gtgtttgggt ttcggtgagg gcactgaagg cttcttgcgc catctttatg tcggattctg
     1981 cgtctttcat aactctttcg aaaacatcag cctctacatc atctttgacc aattccatcg
     2041 ccttgtagaa tgcaggaatg gttgcgatgt ctataaagtt gttaggggtt agacgcccag
     2101 tttggcttaa aaagccttgt cttactgcgc gaatgtcgcg gaaatttatt ttatcatctg
     2161 catctctatc agtttcttgc aggcgccggg caataacttt ggccatctcg ccttgtatga
     2221 gagggcctgc ggttaggtct attagagcgc tgttgatagc gttttgtgcg tctaaacttt
     2281 cgagtttttt gccatcatga gttctgtaag aaaagccatt ttccagttcc gggttgatgg
     2341 taccgtttcg catgtggttc catttgatct ggaaagtgcc ggcctgtgtt tccagcgaaa
     2401 ttgtgttgtt ttcaaggtcc gtttttatcg cttcgcttat tgctatgacc tgaagggcag
     2461 gcatgatttc gtttgcgaaa ttttccctga gcgcttggtt gtcgaattgt tccagaatgg
     2521 cgtgtgcttg ttgcgccgtt ttgctgacaa tttctgtaga gtttggcagc gcgctgagtt
     2581 cggggatttc attgatcgta agcgctgctt tttgcgcatg gtcgtttgga acaagcgcat
     2641 tgagggcgtc gtggttggtg ttgagccagt cgcggacttt gcctagcaaa attttttgaa
     2701 catctggggt gttaaccgcc atcaatatac cctctgttta aacgccgcta atttttaagc
     2761 tttttgtcgt tttaattatt atcgttatgc aaatggtata gaagaaagat taacaaagca
     2821 ttaattcatt gttatttaag ggtaatactg attttatggg gccgtaattg caagttgcgg
     2881 caaggcgatg cggtaattca taccctctgt ggcacaagtg gattgcttcg cttgcgctcg
     2941 caatgacgct ttgtatcctt agcgaggttt tatgcgcttt gctttacgtc ttgtgtgcgg
     3001 ccatggatga ggtcttcgta ggcttcgtgc agtttgcggg tgatggggcc aacgctatag
     3061 gtaatgtcat caattttacc aacggctgtg acttcggcag cggtgccggt gaggaaaatt
     3121 tcatcaaaag attcaagttc ttcgggcttt atacggcgtt cttctacact gatgtttctt
     3181 tcgcgggcca atgacatgac agtttggcgg gtgatgccgt taaggaagcg atcagcgatg
     3241 ggggtgtgga tttgcccatt tttgacggca aagaggttgg cgccggtgga ttctgcgaca
     3301 tagccttcgt gatcaagcat aagggcgtcg gtgtagccgg cttttttgac ttcaaccttg
     3361 accatgcaac ttagattgta gagtgaagcg gttttagccg cggtggggga tgtgttgggt
     3421 gcgggtttgc gccaggtgga cgttttcagg gaaatgcctt tttcacgaat tgccgggtca
     3481 aaatagctgc cccagtccca ggcggcgatg gcgacatggg tttttgttcc ggcaacgtcg
     3541 atgcccattt gttccgctcc gcgccatgct gctgcacgaa tataggcgtt ttcgaggttg
     3601 ttggctttaa gaacttcttg cttgatgttt tcgagttcat ctacggaaac cggcatgtcc
     3661 atatagatga ttttggcaga gttatgcagg cgctgcgaat gctcgcggct tttgaagatg
     3721 tggccgtcat aggcgcgttc accttcaaag acttgtgtgc cgtaatgcag agcgtgggta
     3781 agatagtgta ttttggcttc gcgccaaggc agcatttttc catccatcca gataaagcca
     3841 tcacgatcat cgtaaggaat taaagccatt tgaggtgtac tccgcgttaa attcggctat
     3901 aaattacggt aaactaacag gatttgtaaa ccacttcatg atgatgagca agagttcgca
     3961 agaaaattac ccgaaagatg gcggttcgta tgaggcttgg ccgcatgttc tggtggttga
     4021 tgatgaccgg cgcattcggg atttgctgtg ccggtatttg agtgagcaag ggtttgtggt
     4081 gattgcggcg gcagatgcgt ttgaggcgac ggcgttgctt gaaagctttg aggtggatgt
     4141 gcttgtggtg gatgtgatga tgccgggcaa gacggggctg gaatttacag ctggataccg
     4201 ggagagcggc tcggtagtgc cgattttgtt actgacagcg ctgggggaaa cagaagaccg
     4261 gattacgggt ctggaagcgg gggcggatga ttatttgtcc aagccttttg agccgcgaga
     4321 attggtgttg cgtcttaatg cgattttgaa gcgcacagcc aagcctgtgc acgtggagcg
     4381 aagcctaaag attgggccgt gggtgtttga tccggtgcat gaggagctta gcgcaggcgg
     4441 tgatgttgtg cggttgacaa gcgcagaggc gcagctgctt aaggcgttgg gcgcgcgtg
//
LOCUS       DAOSWR010000166         4431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13413_length_4431_cov_4.429159, whole genome shotgun sequence.
ACCESSION   DAOSWR010000166 DAOSWR010000000
VERSION     DAOSWR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13413_length_4431_cov_4.429159"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK513_09860"
     CDS             complement(<1..336)
                     /locus_tag="PK513_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOO82796.1"
                     /translation="MFPGSLGQSLAGKALERGDWGYECVNIRDFGEGVHKTVDDTPYG
                     GGAGMVMYADVIEKALLSIQPQLSSRRRPGSGVGNKKIPDQVRDDSMRLIYLSPRGKP
                     LTQSLVKELS"
     gene            complement(372..1196)
                     /gene="rimM"
                     /locus_tag="PK513_09865"
     CDS             complement(372..1196)
                     /gene="rimM"
                     /locus_tag="PK513_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOO82797.1"
                     /translation="MTNTVKTARTLRKNQTDAEKLLWSKLRNRQFENLKFRRQHPVPP
                     YVADFYCEDLKLIVEIDGGQHTPEKDRERQKYIEDQGYKIIRFWNNDVLSNIDGVLEK
                     LSLSIPSPKPSPQGEGLTSRICVGRIASAHGVKGLVKLLPFCEDPALLEQALDFKITL
                     KNPMGKYMLAEIEGVKDRNAAEALRNTELFVTRDQLPEIDDDDTFYFEDLIGMTCVDE
                     NGMEIGHVKAVENFGAGDLLEVQLKSGQEVLLPFTDEYVPEIGETVTIRNYERLML"
     gene            complement(1317..1844)
                     /gene="rpsP"
                     /locus_tag="PK513_09870"
     CDS             complement(1317..1844)
                     /gene="rpsP"
                     /locus_tag="PK513_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOO82798.1"
                     /translation="MALAMRLSRGGRKARPFYRIVVADKRMARDGRYIEKLGTYTPLL
                     DDKDENRVKLVEDRIKYWLSQGAQPSERVAIFLGKAGIIDMPAQPNRPKKSEQSEKTK
                     LRIEDKKEKQTAAAEAAKAAEEEAKAAAEAAAAAPGAPAEDAAPAETEAPAEDAKVEE
                     TPAEEPKAEDEEKAE"
     gene            complement(1872..2216)
                     /locus_tag="PK513_09875"
     CDS             complement(1872..2216)
                     /locus_tag="PK513_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82799.1"
                     /translation="MDKLSQRNIYVIRFLILVVHTLIPAIPVIVAGWLAFSLYDFFGI
                     GIIAHASAYALAYFVSLFFCIRSIKRQNLKAATINTAITSVLNIAVFLLCLFPRDSQP
                     HNVKRTFKTHEL"
     gene            complement(2340..3911)
                     /gene="ffh"
                     /locus_tag="PK513_09880"
     CDS             complement(2340..3911)
                     /gene="ffh"
                     /locus_tag="PK513_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOO82800.1"
                     /translation="MFESLSEKLGGVFSKLRGKGALSENDVMAVSREIRVALLEADVA
                     LPVVKDFIASIKDKAVGQDIIKGVNPAQQVIKIVHDELVEMLGSETDALKFSSPPSVY
                     LMVGLQGSGKTTSTAKISKLLSDKQNKKILMASLDVYRPAAQEQLKQLGEQTEIATLP
                     VVEGQKPLAITKRAMDTARKEGYDVVMLDTAGRLSIDEALMDEVAAIQKEAKPIETLL
                     VADAMTGQDAVQTAKAFDERVPLSGIMLTRVDGDARGGAAMSMKAVTGKPIKLIGVGE
                     KWDEIETFHPERVAGRILGMGDVVSLVEKAVETVDSEEALKMAKKFKKGQFDFNDLLS
                     QIQQMKKMGGMGAMMKLMPGLSSMAGKLDEAGLDDGFVKRQEAIIYSMTKAEREKPDL
                     LNMRRKQRIATGSGTSVQDVNKIVKQLKQMQTMMKKMRKMGTGKMMGMMKDMMGGKAD
                     ELELMAGSMDPDALGHDMAGLGQSGGLGPNPFTGGAGGLPGLGGTGLPGLGGGMPGLP
                     SHGGKKKDRKKGKRK"
     gene            4161..>4431
                     /locus_tag="PK513_09885"
     CDS             4161..>4431
                     /locus_tag="PK513_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO82801.1"
                     /translation="MMRFKKMHGLGNDFVIIDTRTQSVTIGHARIQDICDRHFGVGCD
                     LLALIEPSEKADVFARFYNADGSESAACGNATRCIADIVMKERGTD"
BASE COUNT         1012 a         1259 c         1023 g         1137 t
ORIGIN      
        1 gctcagctct ttaacaagac tttgcgtcaa aggtttcccg cggggagaga ggtaaattaa
       61 cctcatgctg tcatcccgga cttgatccgg gatctttttg ttacccacac cagatcccgg
      121 cctgcgccgg gatgacaatt gtggttggat cgaaagcaac gctttctcga tcacatccgc
      181 gtacatcacc atgcccgcac ctccgccata gggtgtatca tccacggtct tgtgcacgcc
      241 ttcaccaaaa tcacggatat tcacgcactc ataaccccaa tccccacgct ctagagcttt
      301 cccggccaaa gactgcccaa ggcttcccgg aaacatttca ggaaagagtg tcagaatgtc
      361 gaaatgcaga ctcacagcat cagcctttca taattgcgga tggtcacagt ttcgccaatt
      421 tcaggaacat actcatcagt aaaagggagc agcacttctt gcccggattt aagctgtacc
      481 tccagcaaat cacccgcgcc aaaattctcg accgctttca catggccgat ctccatgcca
      541 ttttcatcaa cgcaagtcat gccgataagg tcttcaaaat agaaggtatc atcatcgtca
      601 atttcaggga gttgatcgcg cgtaacaaac aactcagtgt ttctaagcgc ttcggcggcg
      661 ttgcggtcct tcacgccttc tatttccgcc agcatatatt tgcccatcgg atttttgagc
      721 gtgattttga aatcaagggc ctgctcaagc agggcagggt cttcgcaaaa cggcaaaagc
      781 ttgaccaacc ccttcacgcc atgtgcgctg gcaatcctgc cgacgcatat acggctcgtt
      841 aagccctctc cctggggaga gggtttgggt gaggggatac tgaggctgag tttttccaaa
      901 acaccgtcta tattgcttaa aacatcatta ttccaaaaac gaataatctt atacccttga
      961 tcctcaatat atttttgacg ttctcgatct ttctcaggcg tatgctgacc accatcaatt
     1021 tcaacaataa gcttgagatc ctcgcaataa aagtctgcga catagggcgg aacgggatgc
     1081 tgacgacgaa atttgaggtt ttcaaattgc cggttgcgca gtttcgacca gaggagcttt
     1141 tcggcgtctg tttggttttt ccgaagtgtt ctggcagttt ttacggtatt ggtcataatt
     1201 cccctcaccc actgcggcta aagcgcttca cgcttaagcc tcgtaatccc tctcccttag
     1261 ggagagggtg gtctgagcga aagcgaagac cgggtgaggg aggatacttc acgagattac
     1321 tctgcttttt cttcgtcttc agccttcggc tcttcagcag gagtttcttc aactttagcg
     1381 tcctcagccg gagcttccgt ttcggcaggc gcagcgtctt cagccggtgc tccaggtgca
     1441 gccgccgcag cttccgctgc cgctttggct tcctcttcag cagctttagc cgcttcagca
     1501 gccgcagttt gcttttcttt cttgtcttca atccgcagct tggtcttttc agactgctct
     1561 gattttttcg ggcggtttgg ctgcgcaggc atgtcgataa tcccggcttt accgaggaaa
     1621 attgctacgc gttcggatgg ttgcgcccct tgggatagcc agtatttaat gcggtcttca
     1681 accagtttca cgcggttttc atccttgtca tccaaaagcg gggtatacgt gcccagcttt
     1741 tcaatatagc ggccatcgcg cgccatgcgc ttgtccgcca caacgatgcg gtaaaatgga
     1801 cgggcctttc gtccgcctct ggatagtctc attgcaagtg ccatttcatt ttctccttta
     1861 attaatgttg gttacaattc atgagtttta aaagtccttt tcacattgtg tggttgagaa
     1921 tctcttggaa aaagacaaag taaaaaaaca gctatattaa gaacactggt gatagcggta
     1981 tttatcgtcg cggcttttag attttgtctt ttgatggagc gaatacaaaa aaacaacgat
     2041 acaaaataag ctaaagcgta ggcgctggca tgagcaatga taccaatacc aaaaaagtcg
     2101 tataacgaaa acgccagcca tcctgctacg ataacaggaa ttgccggtat gagcgtatgc
     2161 acaaccagaa taagaaacct gataacatat atgttacgtt gtgacagttt atccatcgtt
     2221 tttcccctcc attctgctgt cattgcgagg aggccgtagg ccgacgcggc aatccagact
     2281 ggattgcttc gcggagttta tgcccctgag ggtgctcgca atgacggggt aggcgaatgt
     2341 cattttcgtt tccccttctt gcggtccttt ttcttaccgc catgagaggg aaggccgggc
     2401 atgccgccac ccaaaccggg cagacccgtg ccgccaaggc cgggcaaacc gcccgcgcca
     2461 ccggtaaacg ggtttggccc caaaccccca gactggccaa ggcccgccat atcatgaccc
     2521 agagcatccg ggtccatcga cccagccatc agctccagct catcagcttt gccacccatc
     2581 atatccttca tcatgcccat cattttgccg gtgcccatct tgcgcatctt tttcatcatc
     2641 gtttgcatct gcttgagctg cttcacgatt ttgttaacat cttgcacaga tgtcccggac
     2701 ccggtcgcaa tgcgctgctt gcgcctcata ttaagcaaat cgggtttttc gcgctcggcc
     2761 ttggtcatgg aataaataat cgcctcctga cgcttcacaa aaccatcatc cagtcccgcc
     2821 tcatcaagct tgcctgccat gctggaaaga ccgggcatca gcttcatcat cgcgcccata
     2881 ccgcccattt tcttcatctg ctgtatttgc gagagtagat cattaaaatc aaactggccc
     2941 tttttgaact ttttggccat tttcagggct tcttcggaat ccaccgtctc cacggccttt
     3001 tccaccaaag acaccacatc gcccatcccc aaaatgcgcc ctgcaacccg ctcaggatgg
     3061 aaggtttcaa tctcgtccca tttctcgcca accccgatca gcttgatcgg cttgccggtc
     3121 acggccttca tgctcatcgc cgccccgccg cgcgcatccc cgtctacgcg cgtgagcata
     3181 atgccggaaa gcggcacgcg ttcgtcaaaa gccttggccg tttgcaccgc gtcttgcccg
     3241 gtcatcgcat cggccaccag cagcgtttca attggcttgg cctctttctg gatcgccgca
     3301 acctcatcca tcaacgcttc gtcaatggac aaccgcccgg cggtatcgag catcacaacg
     3361 tcatagccct ctttacgggc cgtatccatc gcgcgctttg tgatggcgag cggcttttgc
     3421 ccttcgacaa ccggaagcgt cgcaatctcc gtttgctcac caagctgctt gagttgttcc
     3481 tgcgccgccg ggcggtaaac gtcgagcgaa gccatcagga ttttcttgtt ctgcttatcg
     3541 ctcagcagct tggaaatctt cgccgtcgag gtcgtttttc ccgaaccctg caaacccacc
     3601 atcaggtaca cggagggcgg ggaagagaat tttagagcat cggtctcact gcccagcatt
     3661 tcaaccagtt cgtcatgaac aatcttaatc acctgctgcg ccgggttcac acccttgatg
     3721 atgtcctgac caacagcttt gtctttaatc gaggcaatga aatctttgac gacgggcagg
     3781 gcgacatcgg cctccagcag cgcaacgcgg atctcacggc tcaccgccat gacatcattt
     3841 tcgctcaaag cccctttgcc tctgagtttt gaaaatacac caccaagctt ttcgctcaaa
     3901 gattcaaaca tctgcaaacc gttattaaca ataaatctta gccccgctga gcgttcgcgc
     3961 cgagcgaggg gtaccgttaa gcactgactt caaaacaaac atgtcagtga aatttgggtg
     4021 gaaaatatgg gatatgccct atattgtcaa ggactttaac gctttgcgtc attgcgagcg
     4081 gagcgaagca atccagtaga ccacatggat tgccacgtca ggcctccggc cctcctcgca
     4141 atgacgatgt tgggaaatga atgatgcgct ttaaaaaaat gcacgggctg gggaatgatt
     4201 ttgtaatcat agacacgcgc acgcagtctg taaccatcgg ccatgcgcga atacaagaca
     4261 tttgcgaccg gcatttcggt gtcggctgtg acttgctggc acttattgag ccttctgaaa
     4321 aggccgatgt ctttgcccgc ttttacaatg ccgacggttc tgaaagcgcc gcctgcggca
     4381 atgcaacgcg ctgcatcgcc gatatcgtga tgaaagagcg cggcactgat c
//
LOCUS       DAOSWR010000167         4418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13461_length_4418_cov_4.723585, whole genome shotgun sequence.
ACCESSION   DAOSWR010000167 DAOSWR010000000
VERSION     DAOSWR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13461_length_4418_cov_4.723585"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PK513_09890"
     CDS             <1..1829
                     /locus_tag="PK513_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82802.1"
                     /translation="TAKLDPSGGEIQNMGQLPAHEVSQRFSGVVGVSPTLFSSINDAA
                     AEQGTQPVNGPQEQPRENFDAEGTLDHNNDGEVDGSVEGGDDAAGNGDGAPLDGAKDP
                     ALMDGEPVKLTGEDGLAPKPMMNPMQTGMMSTDPMGMHPMGMNPTGTMNATAGMETLD
                     GGKNTMNRMNLLRNMANGDHANGDGILPSPPTGENMQDPSTIIPQNGGNDTTPPKHVS
                     DLNSFAQTQISTAPNEFFATSDNMIWDYHFDKEFIDPDGGDLHFELSAGTKDILNTTS
                     GITTWTFDNANGHLTINTASLPGDFNFNIQVRAIDQAGNAGSFENYTFHLEQKTGTFP
                     STVDSTGTDVLTGSANADTTTLGGSSTTTNKTIFAGDGDDNILIYDYGYNNTIHLGNG
                     ENIFVVTDGATGNTAIGGFEKDTFNMGGVQNHAIGMDGDDIFKINFSTGPAKTELETS
                     GNSNILMDGGHSSYRMPFLLHGQTPPASELGRGDTLSLEGTGVELDFTLVDNNYFRGI
                     ERIDLLNGSTTGAGITLGLNDVINMTDHNNILLIRADSNDCLTFVPGDFANFTKTENK
                     YLDDNLTYTATTGDDTNFTMYSNGNVTLLIEDNGATVSGLPA"
     gene            complement(2033..2761)
                     /locus_tag="PK513_09895"
     CDS             complement(2033..2761)
                     /locus_tag="PK513_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO82803.1"
                     /translation="MNMGLAKSTAASAVVFFAVAAADMVTNKAQAAFIKLNFDVSNFE
                     NSFGPVPAGITGTEGTIRIDTGTGTDLSSIPSLALLTGVDVSGSFQAGGETIGISSSP
                     FMVVDDGGISGTTDQLRLLFDAEANSWGITGVQVGLTGDYTVFTGKDLGTILLDASSL
                     NSLNDRFAQLRFDNIGGVLETNNPELSFAEVNANTVPEPAVPALLGAGLAGLGLALRR
                     REDDVVENNDNGPGADMYSLPRIN"
     gene            complement(2904..4388)
                     /gene="glpK"
                     /locus_tag="PK513_09900"
     CDS             complement(2904..4388)
                     /gene="glpK"
                     /locus_tag="PK513_09900"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOO82804.1"
                     /translation="MSKAETILAIDQGTTSSRAILFSVGGAVLDVQQKELKLYYPEKG
                     WVEQKPDDIWDDTRTACAKILEKCEKRPAAIGITNQRETTIVWDRETGEAVYNAIVWQ
                     DRRTAEVCAALKDQGYEAMVASKTGLLLDPYFSATKIAWILEHVPGARARAEAGELAF
                     GTVDCYLLWKLTGGKVHATDVTNASRTMLYNIVEQCWDDDLLALFDISASLLPEVRDN
                     IADFGVSDEETIGAALPIGGMAGDQQAALIGQGCLTPGMIKSTYGTGCFVLINIGSEF
                     RRSQNRLLTTPAYRVDGQIQYAIEGSIFVAGAALQWLRDGLELFEDARETESLAYSVS
                     DNNGVYFVPAFTGLGAPHWKPDARAMITGMSRESTKAHVVRAALEAQAYQTLDLLGAM
                     EDDGGHKADVIRIDGGLAANGFVCQFLADMLQRPVEVPAVVETTAWGAATLAGVQAGV
                     FKDLEAVAQGWKAARRYGPQMGAEARDALYDGWKAALSMLLQEN"
BASE COUNT         1283 a         1322 c          904 g          909 t
ORIGIN      
        1 aaacagcaaa gctcgaccca tccggcggtg aaatccagaa tatgggacaa ttgccggcac
       61 atgaagtttc acaaagattt tccggggtcg taggcgtatc tccgacgcta ttttcatcca
      121 tcaacgatgc cgccgccgaa caaggaacgc aacctgtcaa cggaccgcaa gaacaaccgc
      181 gtgaaaattt cgacgcggaa ggcacactcg accacaataa cgacggcgaa gtcgatggct
      241 cggttgaagg cggtgacgat gccgcaggca acggcgatgg cgcacctctg gatggtgcca
      301 aagacccggc cttaatggat ggcgagcctg taaaattaac cggcgaagat ggcctcgcgc
      361 ccaaaccgat gatgaatcct atgcaaaccg gcatgatgag cacagacccc atgggcatgc
      421 acccaatggg tatgaatccg accggaacga tgaacgcaac cgcaggcatg gaaacgcttg
      481 acggtggcaa aaacaccatg aacagaatga atctgctaag gaatatggcc aacggcgacc
      541 acgcaaacgg tgacggcata ctgccgtcac cgccgaccgg agaaaatatg caagatccaa
      601 gcacaatcat tccacaaaac ggcggaaacg acacaacccc gccaaaacac gtcagcgatt
      661 tgaactcatt cgcacaaact caaatcagca ctgcgccgaa tgaatttttc gcaacatcag
      721 acaatatgat ctgggattat cattttgata aggaatttat tgatccggat ggaggagatt
      781 tacactttga attaagtgcg ggcacaaaag acatactcaa cacaacatca ggaattacaa
      841 cttggacttt tgataacgca aatggccacc tcacgattaa taccgcctca cttccaggtg
      901 actttaattt caatatccag gtccgcgcca ttgatcaggc aggaaacgcc ggcagttttg
      961 aaaactatac gttccaccta gaacaaaaaa ccggaacttt tccatcgacg gttgacagca
     1021 caggaacaga cgttttaacc ggaagcgcca atgcagacac cacaacacta gggggatcaa
     1081 gcaccacaac caacaaaacc atttttgcag gcgatggcga tgacaacata ctcatatatg
     1141 attatggata caataacaca attcatctgg ggaatggtga aaacatattc gtagtcacag
     1201 acggagcaac cggaaacacg gccattggcg gatttgagaa agatacattc aacatgggcg
     1261 gtgtccaaaa ccacgccatt ggcatggacg gcgacgatat ctttaaaatt aacttctcaa
     1321 ccggcccggc gaaaacagaa ctggaaacct caggaaactc caacattctc atggatggcg
     1381 gccacagctc ctaccgcatg ccgttcctgc tacacggcca aacaccgccg gcaagcgaac
     1441 ttggccgcgg cgacaccttg tcactggaag gaaccggggt agagctggat ttcacgctcg
     1501 ttgacaataa ctatttccgg ggaatagagc gcatagatct cttaaacgga agcacaaccg
     1561 gagccggcat aacactcgga ctcaatgatg tcatcaacat gaccgatcac aacaacattc
     1621 ttcttatccg cgccgacagc aatgactgtc taacttttgt tcctggagat tttgcaaact
     1681 tcacaaaaac agaaaataaa tatctggatg ataacctcac atacacagcc acaacaggag
     1741 acgacaccaa cttcaccatg tattcgaatg gaaacgtcac ccttctgatt gaggacaacg
     1801 gcgcaactgt aagcggactt cccgcataaa cacaacgtct aaaaaaacac ataaaaaaag
     1861 accgccctga atgcgcggtc tttttaaatg atattgacga aaaattatag cctacatcat
     1921 ataaaatgat cataaaaacc gtcattgcga ggaagcgaag ctgacgaagc aatctaggtt
     1981 ttccgctgga ttgccgcgct ccctacggtc gctcgcaatg acgaaaattg aatcaattta
     2041 tccggggtag gctatacata tccgcgcccg gcccattgtc attattttca acaacgtcat
     2101 cttcacgacg gcgcagtgcc aaacccaaac cagcgagccc cgccccgaga agagcaggca
     2161 ctgcaggctc tggaacggta ttagcattaa cttcagcaaa ggaaagctcc gggttgttgg
     2221 tctcaagtac accgcctata ttatcaaatc gaagctgagc aaatcgatca ttcaaactat
     2281 tcaaactaga cgcatccaac aaaatagtgc ctaaatcctt gccagtaaac accgtataat
     2341 cgccagttaa accaacttgt acaccagtaa tgccccagga attagcctct gcatcgaaaa
     2401 gcaaacggag ctgatcggtt gttccggata ttccgccatc atccacaacc ataaaaggag
     2461 aggaagaaat acctatagtc tcaccgccag cctggaaact cccggaaaca tcgacccctg
     2521 taagcaaagc aagggacgga atcgaagaaa gatccgtccc ggtgccagtg tcgatccgaa
     2581 tagtcccctc agttccagta atgcctgctg gaaccggccc gaaagaattt tcaaaatttg
     2641 aaacatcgaa attcaactta ataaacgccg cctgcgcttt attcgtcacc atatctgccg
     2701 cagcgacagc gaagaacacc accgcacttg ctgctgtact ttttgctaaa cccatattca
     2761 ttattctgcg tctcccattt catttacgca acgtattcaa aacttttaaa tcaaaaatgc
     2821 tataagatca atcaactata gagcgaacca tcaacattgg gcgcttttat atagtaaatt
     2881 tacgtaaaac aaaagaagtt ttttcaattt tcttgcaaaa gcatactcaa agccgctttc
     2941 cagccatcat agagcgcatc ccgcgcctcg gcgcccatct gcggcccata acgccgcgcg
     3001 gccttccacc cctgcgcaac cgcttctaaa tccttaaaca ccccggcctg caccccggcg
     3061 agcgtagccg cgccccacgc cgttgtttcc accaccgccg gaacttcaac cggcctttgg
     3121 agcatatcgg ccagaaactg gcagacaaag ccgttggccg ccagcccgcc gtcaatgcgg
     3181 ataacatcgg ctttatgccc gccatcatcc tccatagccc ccagcaaatc cagcgtctga
     3241 tacgcttgcg cctccagcgc cgcgcgcacc acatgcgctt ttgtgctctc ccggctcata
     3301 cccgtaatca tcgcccgcgc atccggcttc cagtgcggcg cgcccagccc cgtaaaggcc
     3361 ggcacaaaat acaccccgtt attatcggaa acgctatatg ccagactttc cgtctctcgc
     3421 gcatcttcaa acagctccaa cccgtctcgc aaccactgca gcgccgcccc ggccacaaag
     3481 atcgagcctt caattgcgta ttgtatctgc ccgtccactc ggtacgcagg cgtcgtcaga
     3541 agccgatttt gcgagcgcct aaactcactc ccaatattta tcagcacaaa acaccccgtg
     3601 ccataggtcg atttaatcat ccccggtgtt aagcagccct gcccgatcag cgccgcttgc
     3661 tgatccccag ccatcccgcc aatcggcagc gccgcaccga tggtctcttc atcactcacg
     3721 ccaaaatccg cgatattatc ccgcacctcg ggaagcaagc tcgcagaaat atcaaaaagt
     3781 gccaacaaat catcgtccca gcactgctcc acgatattat acagcatcgt gcgcgatgca
     3841 ttcgtcacgt ccgttgcatg aactttcccg cccgtaagct tccacagcaa ataacaatcg
     3901 accgtgccaa acgccaattc acctgcttca gcccgcgccc gtgcaccggg aacatgttcc
     3961 aaaatccacg cgatcttcgt cgccgaaaaa tacggatcca aaagcagccc cgtcttactc
     4021 gcaaccatcg cctcatagcc ctgatctttc aaagccgcac aaacttccgc cgtgcgccga
     4081 tcctgccaga caatcgcatt ataaaccgct tcccccgtct cccggtccca aactatcgtc
     4141 gtctcacgct gattggtaat cccgatcgcc gccggacgct tttcacattt ttctaaaatt
     4201 tttgcgcaag ccgtccgcgt atcatcccaa atatcgtcag gcttttgctc aacccagcct
     4261 ttttcaggat aatacaactt aagctctttt tgctgcacat ccaaaaccgc accaccgaca
     4321 gaaaataaaa tcgcccgact actcgtcgta ccctgatcaa tcgctaaaat cgtctcggcc
     4381 ttactcacgc cagcaccttt ttcttcaaac ccggcaac
//
LOCUS       DAOSWR010000168         4303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_13991_length_4303_cov_5.691384, whole genome shotgun sequence.
ACCESSION   DAOSWR010000168 DAOSWR010000000
VERSION     DAOSWR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_4303_cov_5.691384"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1058)
                     /gene="trpS"
                     /locus_tag="PK513_09905"
     CDS             complement(51..1058)
                     /gene="trpS"
                     /locus_tag="PK513_09905"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO82805.1"
                     /translation="MKRILSGMQPTSHLHLGNYLGALKNWVALQEEPDSQCLYMVADL
                     HAVTVPQEPEALTLATREIAAAFIAGGIDPSKSAIFVQSAVPGHSELMWLLATMTQMG
                     KLERMTQFKDKAGKNAERAGLGLFAYPVLMAADILLYKTTHVPVGEDQKQHLELARDI
                     AATFNARFGGDVLLPPEPYIVGEATRVMSLQDGSKKMSKSDDSEKSRINLTDDADIIA
                     KKIKKAQTDPDPLPEAVEGLEGRAGAANLVTIYAALAGISKADVLAEYAGQGWGVFKP
                     ALAELAVEKLSPITARMNELMADPAQIDAILAQGNAKANEIANRTVAEVRDIMGFWSA
                     H"
     gene            complement(1061..2596)
                     /gene="murJ"
                     /locus_tag="PK513_09910"
     CDS             complement(1061..2596)
                     /gene="murJ"
                     /locus_tag="PK513_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOO82806.1"
                     /translation="MKLAKAMATVAGLTGLSRIFGFIRDIMTAAILGAGPVADAFFVA
                     LKLPNFFRRVTAEGAFSVAFVPLYTESVEKEGQEEADAFASNVFMVMLSMLSVVVLLA
                     LAGMPWVISAIAPGFIDDPVRFDAAVQLSRITFPYLLLMSLTALLGGVLNALNRFAPF
                     AFAPVLFNLCLISALLLSGYFADAGQALSFGVLFAGVLQLIWLVVAARRSGVYLRFKR
                     PVFDVKIKRVFKLMGPGVIGAGVMHINLFADMVIASFLSAGSISYLYYADRLNQLPLG
                     VVGIAVGTALLPMLSKALAKGDMDEGRDLFNRALEMCLLLALPAAAALAFFGRDLVGG
                     LFERGAFDYQDSQATVMVLTGYAFGLPAYICVKVFSTVHWASQDTKTPVKISVIATVI
                     NIALSLLLIQYIGVAGIALATGLTGWLQFIFHVKALKGKRVEFDDRLKRASLKIFWAT
                     GLMVGGVVFLMLAIDNDYAMSKMQRMIITVVSGLGIYSVLVLVFGLVNLDQLKRFLKK
                     KKV"
     gene            2711..3457
                     /gene="ubiE"
                     /locus_tag="PK513_09915"
     CDS             2711..3457
                     /gene="ubiE"
                     /locus_tag="PK513_09915"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOO82807.1"
                     /translation="MQNPESTQFGQRTVTPEEKTGLVRGVFDSVADNYDLMNDLMSGG
                     LHRLWKDTFVRRIRPKNNMKFLDVAGGTGDIAFRIKKKSPGSDITVFDLNPNMLEVGR
                     DRAIDRGWINDFEWITGNAANLPFEDNSFDVYTIAFGLRNVTLIDEALREATRVLKPG
                     GRFFCLEFSHVREPLLAKAYDLYSYNIIPRIGQIVAKDRDSYQYLVESIRKMPQQEEL
                     KNRMENAGLKNCSFSNLSAGIVAIHRGTKL"
     gene            3586..4098
                     /locus_tag="PK513_09920"
     CDS             3586..4098
                     /locus_tag="PK513_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82808.1"
                     /translation="MNNFTKSIVYSGVVLAAGLVAIFAIYNNMGTSGNNISYIEPAAG
                     SEAAEGIAEGVSGFASSIQEAAGEAAETVSEMTETTMETAADAADATAEASDDVYDAM
                     AEIADDAVEATEEAASETTEAIEDAAATNPDEETAETIEHAADSIEDTIETEPASGDA
                     IIEKAKEMMQ"
     gene            4170..>4303
                     /locus_tag="PK513_09925"
     CDS             4170..>4303
                     /locus_tag="PK513_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOO82809.1"
                     /translation="MTQNLKEKSILLIISGGIAAYKSLELIRLLKKAGCTVRCILTKG
                     G"
BASE COUNT         1263 a         1287 c          852 g          901 t
ORIGIN      
        1 gattatgcct caaacacaac cattttgcca gagggaatat gaataaccgg ttaatgggcg
       61 gaccaaaagc ccataatatc ccgaacttca gctacggttc tgttcgcgat ttcattcgct
      121 tttgcgttgc cttgggccaa aatcgcatca atctgcgccg ggtcagccat taattcattc
      181 atccgtgccg ttataggaga aagcttctcc actgcaagct cagccagagc aggcttaaat
      241 acgccccagc cctgcccggc atattccgcc agcacatccg ccttcgaaat acccgccagc
      301 gcggcataaa tcgtcaccag attggccgcc cccgcgcgcc cttcaagccc ttcaaccgcc
      361 tcaggcaatg gatcagggtc agtctgcgcc ttcttgattt tcttggcaat tatgtccgca
      421 tcatcagtca gattaatccg cgatttctca ctatcatccg acttgctcat cttcttgcta
      481 ccgtcttgca aactcatcac ccgcgtcgcc tcaccgacaa tatacggctc aggcggcaac
      541 aaaacatccc cgccaaaacg cgcattaaat gtcgcggcaa tatcgcgcgc cagctccaaa
      601 tgctgctttt gatcctcgcc caccggcaca tgcgtggtct tatataataa aatatccgcc
      661 gccatcagca ccggataggc aaacagcccc aaacccgccc gttcggcgtt cttccctgcc
      721 ttatccttga actgcgtcat gcgctccagc ttgcccatct gcgtcatcgt ggccaaaagc
      781 cacatcagct cagaatgccc cggcaccgca gactgtacaa aaattgccga ttttgacgga
      841 tcaattccac ccgcaataaa cgccgccgca atctcgcgcg tcgccaatgt caaagcctcc
      901 ggctcctgcg gcacagtaac cgcatgcaaa tccgccacca tatacaaaca ctggctatcc
      961 ggctcttcct gcaaagccac ccagtttttc agcgccccga gataattccc cagatgcaaa
     1021 tgtgatgtcg gctgcatacc tgacaaaatg cgtttcattc ttatactttc tttttcttta
     1081 aaaatcgctt tagctgatca agattcacta acccaaaaac caaaactaaa acactataaa
     1141 tccccaaacc tgaaaccaca gttatgatca tccgctgcat tttactcatt gcataatcgt
     1201 tatcaattgc cagcatcaga aaaacaaccc caccgaccat caaaccagtc gcccaaaaaa
     1261 ttttcaatga cgcccgtttg agcctatcat cgaattctac gcgttttcct ttaagggctt
     1321 taacatgaaa aatgaactgc aaccaacccg ttaatcctgt tgcaagggca atgcccgcta
     1381 ctccgatata ctgaataagc agcaaactca acgcaatatt gataacagta gcaataacgg
     1441 aaatcttcac aggtgtttta gtatcctgag aagcccaatg aacagtagaa aaaactttta
     1501 cacaaatata ggctggcaag ccaaaagcat agccggttaa gaccatcact gttgcctgac
     1561 tatcctgata atcaaaagcc cctcgctcaa acaaccctcc aacaagatcg cgaccaaaga
     1621 atgccagagc cgccgccgca ggcagcgcca gcaacaaaca catctccaag gcgcggttaa
     1681 acaaatcccg cccctcatcc atatcaccct tggccagcgc cttagagagc atcggcaaca
     1741 atgccgtgcc caccgcaatc cctaccacgc ccagcggtaa ctgattgagc cggtcggcat
     1801 aataaagata ggaaatcgaa cccgcactca ggaaagacgc aatcaccata tcagcaaaca
     1861 aattgatatg catcacgccc gcgccaatca caccggggcc catcaactta aacacacgct
     1921 taattttcac atcaaatacg ggtctcttga accgcaaata caccccggac cgccgcgccg
     1981 ccacaaccag ccagatgagc tgcaaaacgc ccgcaaacaa caccccaaaa ctgagcgcct
     2041 gcccggcatc agcaaaatac ccactcagca acaacgccga aatcagacaa aggttaaaca
     2101 gcacaggagc aaacgcaaac ggcgcaaacc ggttcagcgc attcaaaacc ccgcccaaga
     2161 gcgctgtcaa cgacatcaac aacaagtaag gaaacgtaat tcgcgaaagc tgaaccgccg
     2221 catcaaaccg caccgggtca tcaataaaac ccggcgcaat cgcagatata acccacggca
     2281 tcccggccag agccagcaaa accaccactg acaacatcga caacatgacc ataaacacat
     2341 tactggcaaa cgcatcggcc tcttcctgcc cctccttctc tacgctttcc gtataaagcg
     2401 gcacaaaggc gacactaaat gcgccctccg ccgtcacccg gcgaaagaaa ttcggcaatt
     2461 tcagcgcgac aaaaaatgca tctgcaactg gccccgcgcc caaaattgcc gccgtcatga
     2521 tatcacggat aaatccaaaa atgcggctaa gccccgtcaa gcccgccacc gtcgccatcg
     2581 cttttgcaag tttcatagaa aactacttac catctgataa tcgtcattgc gaggagcttt
     2641 tgtgacgaag caatccattt taacgaattt ctggattgca tcactccgtc cgcaatgaca
     2701 gtatgccaag atgcaaaacc ctgaaagcac acaattcgga cagcgcactg tcacccccga
     2761 ggaaaaaacc ggactcgtgc gcggtgtatt cgattccgtc gccgacaatt acgacctcat
     2821 gaacgacctg atgtccggcg gcctgcaccg tctgtggaaa gatacgttcg tccggcgcat
     2881 tcgccccaaa aacaacatga aatttctcga tgtcgcgggc ggcaccggcg acatcgcctt
     2941 tcgcattaag aaaaaatcgc ccgggtccga catcaccgtc ttcgatctca accccaatat
     3001 gctagaagtt ggccgtgacc gtgcgattga tcgcggctgg atcaatgatt ttgaatggat
     3061 caccggcaac gccgccaacc tccccttcga ggataacagt ttcgacgtct acaccatcgc
     3121 ctttggtttg cgtaacgtga ccctcatcga tgaagccctg cgcgaagcca cccgtgtcct
     3181 gaaacccggc gggcgcttct tctgtcttga attctcccat gtgcgcgaac cgttactcgc
     3241 caaagcttac gacctctact cctataatat catcccgcgc ataggccaaa ttgttgccaa
     3301 agatcgtgac agctaccaat accttgttga aagcatccgc aaaatgccgc agcaggaaga
     3361 actcaaaaac cgcatggaaa atgcagggct taaaaactgt tcattttcaa atctttccgc
     3421 cggaatcgtc gcaattcacc gtggaaccaa gctgtaaaca caccgctaat ttgccataat
     3481 tacaatggct tgtcgttttt tattgactcg caagtctcga ttgcgcaaaa gtggccattc
     3541 acaaaccact atataactgt gagaattaag gagaggacta ctctaatgaa taactttaca
     3601 aaatcaatcg tctattccgg tgttgtactg gccgcaggtc tggttgcaat tttcgcaatc
     3661 tacaacaaca tgggaacatc tggaaataac atatcataca ttgaacctgc agcaggttca
     3721 gaagccgctg aaggcatagc tgaaggcgtc tctggctttg ctagttcaat ccaggaagca
     3781 gctggcgaag ctgctgaaac tgtttctgaa atgacagaaa caacaatgga aacagccgct
     3841 gatgcagccg atgcaacagc cgaagcttct gatgatgttt atgacgccat ggctgaaatt
     3901 gctgatgatg ctgtcgaagc cacagaagaa gcagcaagcg agactactga agcaatcgaa
     3961 gacgccgcag caacaaatcc ggatgaagaa acagccgaaa ccattgaaca cgcagctgac
     4021 agcattgaag ataccattga aacagagcct gcttccggtg atgcaatcat cgaaaaagca
     4081 aaagaaatga tgcaataaga ctcgctctta aagcaaaagt ttaaaagccc tgctagccat
     4141 agcagggctt tttctttata aacagcgtca tgacacaaaa tcttaaagaa aaatctatcc
     4201 tcttgattat cagcggcggc attgccgctt ataaatcact tgagctcatc cgcttactga
     4261 aaaaagccgg atgcactgtg cgctgcatcc tcaccaaagg cgg
//
LOCUS       DAOSWR010000169         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_14774_length_4149_cov_4.770396, whole genome shotgun sequence.
ACCESSION   DAOSWR010000169 DAOSWR010000000
VERSION     DAOSWR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14774_length_4149_cov_4.770396"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1599
                     /locus_tag="PK513_09930"
     CDS             148..1599
                     /locus_tag="PK513_09930"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOO82810.1"
                     /translation="MKLKNKKLLKDKAYIDGVWVDGPARYDVTNPADGEVIGCAPDLG
                     AGETRAAIAAAEKAFPAWAKKTAKERAAILRRWYELIVEHVDDLAVILTTEQGKPLAE
                     AKGEILYGASFVEWFAEEGKRVYGDLIPSPVAGARISVLRQPVGVSAAITPWNFPSAM
                     ITRKVAPALAAGCTIVCKPAGNTPFSALALAVLADEAGFPAGVFNVITSDDPAPIGRE
                     LCENPAVRKLSFTGSTAVGRLLMRQCADTIKKLSLELGGNAPFIVFDDADIEQAVDGA
                     MACKFRNAGQTCVCANRIYVQDTVYDEFARKLAVKIKALKVGDGLEEGTDIGPMIDEK
                     GMEKVQDHIADALEHGAELVTGGQPHEAGEFFFEPTLLGGMTKEMKIAREETFGPVAP
                     LFRFSDEAEVIDLANDTIYGLAAYFYSKDMARVIRVSEALEYGMVAVNKPILSTEVAP
                     FGGIKQSGLGREGSKYGIDEYLEMKYVLMGGLK"
     gene            1596..2717
                     /gene="ald"
                     /locus_tag="PK513_09935"
     CDS             1596..2717
                     /gene="ald"
                     /locus_tag="PK513_09935"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOO82811.1"
                     /translation="MIIGVPREIKKLEHRVGLVPNSVVEFVRNGHEVWVESGAGAGIN
                     FSDEDYEAAGARIAETAEAVFDHAEMIIKVKEPQPQEFKLLKPHHVLFTYLHLAADRP
                     QAIGLIESGCTAIAYETVTGGDGRGLPLLQPMSEIAGRLSVQVGAYHLQKHKGGEGRL
                     IGGVPGVKPAKVLIIGGGVAGFHAAQMATGLGANVTILERNNERMRFLDEYFIGRANI
                     VYSSTGALEEMAKNADLVIGSVLIPGASAPKLIRREMLSEMIPGSVFVDIAIDQGGCA
                     ETSRATTHDDPTYVVDNVVHYCVANMPGAVPLTSALALNHSVLPYALQLANKGWKQAL
                     SYDLNFLEGLNVCNGKITHRAVAEALDMDLFPTASMLVA"
     gene            2790..2866
                     /locus_tag="PK513_09940"
     tRNA            2790..2866
                     /locus_tag="PK513_09940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2824..2826,aa:Asp,seq:gtc)
     gene            3017..3388
                     /locus_tag="PK513_09945"
     CDS             3017..3388
                     /locus_tag="PK513_09945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOO82812.1"
                     /translation="MAPQDFLLTYLPILLFIVIAAGVSLVMILGSLLAGRQNPDAEKL
                     SAYECGFDAFSDARTKFDVRFYLVCLLFIIFDLEIAFLFPWAISLGEIGVFGFWSMVV
                     FLGVLTVGFIYEWKKGALEWE"
     gene            3385..3726
                     /locus_tag="PK513_09950"
     CDS             3385..3726
                     /locus_tag="PK513_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82813.1"
                     /translation="MTEIQWQEVSQKINALVEMAAQLSAVFRDADENPYRDVSGVYPE
                     TTTQEQYAALDEGTDSRVIEMRKQFEQALALFMKHVSDSGIGEEQLAVLMESTGANDR
                     LDRALQLAGVS"
     gene            3790..>4149
                     /gene="nuoB"
                     /locus_tag="PK513_09955"
     CDS             3790..>4149
                     /gene="nuoB"
                     /locus_tag="PK513_09955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOO82814.1"
                     /translation="MPPAEEQDVVPAAISKTLDEKGFLLTSTDALFDWARTGSMWPMT
                     FGLACCAVEMIHSYMSRYDLDRFGIIPRPSPRQSDVMIVAGTLTNKMAPALRRVYDQM
                     PEPRWVVSMGSCANGGGY"
BASE COUNT          910 a          824 c         1323 g         1092 t
ORIGIN      
        1 ttgggacgag tgatgagagt gcgacggctt gaattcgctc tgttgtcatt gcgaggaggc
       61 cgcatggccc cgacgcggca atccagcaat ggtgcttgca ggctctggat tgcttcgcta
      121 cgctcgcaat gacaataaag ggaaaacatg aaactgaaaa acaaaaagct tcttaaagat
      181 aaggcctata tcgatggggt atgggttgat ggtccggcgc gctatgatgt caccaatccg
      241 gctgatggtg aggtgattgg ctgtgcgccg gatttggggg caggcgagac gcgcgcggcg
      301 attgcggcgg cagaaaaggc ttttccggct tgggcgaaga aaaccgcgaa ggaacgggca
      361 gcgattttgc ggcgctggta tgagcttatt gtcgagcatg ttgatgatct ggcggtgatt
      421 ctgacgactg aacagggcaa gcctttggcc gaggccaagg gggagattct gtatggggcc
      481 agctttgttg agtggtttgc cgaggaaggc aagcgggttt acggcgacct tatcccctca
      541 ccggttgccg gggcacggat tagcgtttta cgccagcctg taggtgtaag tgcggcgatt
      601 acgccgtgga attttccatc ggcgatgatt acgcgcaaag tcgcgcccgc cttggcagct
      661 gggtgtacga ttgtgtgtaa accggcgggc aatacgccgt tttcggcgtt ggcgttggcg
      721 gtgctggccg atgaggctgg gtttccggct ggtgtgttta atgtgattac tagtgatgat
      781 ccggcgccga tcggtcgaga gctttgcgaa aacccggcgg tgcgcaagtt gtcctttacc
      841 ggttcgacgg cggtaggacg gttattgatg cgtcaatgcg cggacacgat taagaagctg
      901 tcgctggagc tgggcgggaa tgcgccgttt atcgtgtttg acgatgccga tattgagcag
      961 gccgtggacg gagcgatggc ttgtaaattc cgcaatgcgg gtcagacctg cgtatgtgcg
     1021 aaccggattt acgtgcagga cacggtttat gatgaatttg ccaggaagct ggcggtgaag
     1081 attaaggctt tgaaagttgg tgacgggcta gaggagggca ccgatatcgg tccgatgatt
     1141 gatgaaaaag gcatggaaaa ggtacaggat catatcgccg atgcgctgga gcatggggcg
     1201 gagcttgtca ccggcggtca gccgcacgag gccggtgaat tcttttttga gccgacattg
     1261 ctgggtggta tgaccaaaga aatgaaaatc gcgcgagaag agacgttcgg tccggtggcg
     1321 ccgctgtttc gtttttctga tgaagccgaa gtgattgact tggcgaatga cacaatttac
     1381 gggttggcgg cgtattttta ttctaaagat atggcgcggg ttatccgcgt atctgaggcc
     1441 ttggaatacg gtatggtagc ggtgaataag ccgattcttt cgacagaagt cgcgccgttt
     1501 ggcggaataa aacagtcggg tctgggtcgt gagggttcta aatacgggat agatgagtat
     1561 cttgaaatga aatatgtatt gatgggaggg ttgaaatgat catcggcgta ccgcgagaga
     1621 ttaaaaagct agagcatcgc gttgggctgg tgcccaattc tgtggtggag tttgtgcgca
     1681 atggtcatga agtctgggtt gaaagcggcg cgggcgcggg gattaacttt agcgacgaag
     1741 attatgaagc cgccggggcg cggatcgctg aaacggctga agcggtcttt gaccatgccg
     1801 agatgattat taaggttaag gagccgcagc ctcaagaatt taaattgttg aagccgcacc
     1861 atgtgctgtt tacctatttg catctggctg cagataggcc tcaggcgatc ggtttgattg
     1921 agtcaggttg cacggcgatc gcttatgaaa ccgtaacggg cggtgacggg cgcggcttgc
     1981 cgcttttgca gccgatgagt gagattgccg ggcggctatc tgtgcaggtg ggggcctatc
     2041 atttgcagaa gcataaaggg ggtgaagggc gtttaattgg cggcgttccc ggtgtgaagc
     2101 ctgcgaaagt ccttattatt ggcggcggcg tggccggatt tcatgcggcg caaatggcaa
     2161 ccgggcttgg cgcgaatgtt acaattttag agcgcaataa tgagcggatg cggtttttgg
     2221 atgagtattt tatcggccgg gccaatattg tgtattccag caccggggcg cttgaagaaa
     2281 tggcgaagaa cgctgatctg gtgatcggtt ctgttctcat tccgggtgct tcggcgccaa
     2341 aattgattcg tagagaaatg ctgagcgaga tgattccagg ctcggtgttt gttgatattg
     2401 caattgatca gggggggtgt gcggaaacat ccagggcgac tacgcatgat gatccaactt
     2461 atgttgttga taatgttgtg cattattgcg tggcaaatat gcctggtgcg gtaccgctca
     2521 cttcagcgtt ggcgctcaat cattcggtgt tgccttatgc gctgcaattg gcaaataagg
     2581 gatggaaaca ggcactttct tatgacctaa atttcctgga aggtctgaac gtttgcaacg
     2641 gaaagattac gcatagagcg gtggctgaag cgctggatat ggatctgttt ccgacagcat
     2701 cgatgttggt agcataattt ttacaattca gccgtaaaga ccgttgacag aacaggcttt
     2761 aacttgataa gaaaacgaac ctcgaatgcg cgggtgtagc tcagttggtt agagcgccgc
     2821 cctgtcacgg cggaggccgc gagttcaagt ctcgtcactc gcgccatttg agattttaaa
     2881 aaattccctt tttaatttga tgaagtgagt acgcggattt taaaaaattt gcgtgaattc
     2941 ccttgaattc cctgtttcct tttgggcatt gcggtgttaa aacggaggca gattaacaca
     3001 cgaattagac ctgctcatgg cacctcaaga ttttctgtta acgtatttac cgattctgct
     3061 gtttattgtg attgcggctg gagtgtcgct tgtgatgatt ttgggatcgt tgctggccgg
     3121 gcggcaaaat ccggatgcgg aaaagctttc ggcttatgag tgcgggtttg atgcgttttc
     3181 cgatgcgcgt acaaaatttg acgtgcggtt ttatctggtt tgcttgctct tcattatctt
     3241 tgatttagag atcgcgtttt tgtttccctg ggccatatct ctgggcgaaa taggtgtgtt
     3301 cggattttgg tctatggtgg tgtttttggg tgttttgact gttggattta tttacgaatg
     3361 gaagaaagga gcgttggaat gggaatgact gagattcagt ggcaggaagt ttctcagaaa
     3421 attaatgctc tggtggaaat ggccgcgcag ttaagcgctg tttttcgcga tgctgatgaa
     3481 aacccttatc gggatgtttc gggggtgtat cctgagacaa ccactcagga gcagtatgcc
     3541 gctttggatg agggtacaga ttctagagtt attgagatga ggaaacaatt tgagcaggca
     3601 ttggccctct tcatgaaaca cgtaagtgat tctggaattg gtgaggagca attggctgta
     3661 ttgatggaaa gtacaggggc caatgatcgt ttggatcggg cccttcagct tgctggggtg
     3721 agttaagaaa tggggcagga caagcatata atcgctgcgc cggcgtacga ttttcgtacg
     3781 gacgtcaaaa tgccgccggc ggaggagcaa gacgtcgtgc ctgcggcgat ttccaagact
     3841 ctggatgaaa aggggttttt gctcacctct acagatgcgc tgtttgattg ggcgcgtacg
     3901 gggtcaatgt ggccgatgac gtttggtttg gcgtgctgcg cggtggagat gatccattcc
     3961 tatatgagcc ggtatgattt agaccgtttc ggcattattc cacggccatc accccgccag
     4021 tccgatgtta tgattgtggc cgggacgttg accaataaaa tggccccggc cttgcgacgc
     4081 gtttatgatc agatgccgga gccgcgctgg gttgtgtcga tgggatcatg cgccaatggc
     4141 ggcgggtat
//
LOCUS       DAOSWR010000170         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_15097_length_4092_cov_5.937825, whole genome shotgun sequence.
ACCESSION   DAOSWR010000170 DAOSWR010000000
VERSION     DAOSWR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15097_length_4092_cov_5.937825"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1530
                     /gene="hisS"
                     /locus_tag="PK513_09960"
     CDS             121..1530
                     /gene="hisS"
                     /locus_tag="PK513_09960"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO82815.1"
                     /translation="MAKQNKIYKPKPVSGFPEWLPEYRAVEQCWLDKIRAVFESYGYC
                     SIETPAVEELEVIAAKGEDVDKEIYVLERLQADPDEKKDARLALHFDQTVPLARYVAQ
                     HFADLTFPFKRYQMQKVWRGERPQMGRMREFYQCDIDVINVDSLPIHFDAEVAAICYE
                     ALEALDMGRVQLRVSNRKILMGFLESLNVPDKKTNGETTVKLSDETKAEIIRDIDKLE
                     KIGKKVLLSRLEQTLLVVGELGFNFQTFIEPFLNLAETKIKPGANILELFQNNSEIDS
                     SNEMLREGIEELQFVLDQLAHLPEHAVVADLSIVRGLDYYTGTVYETQLLDVPEFVGS
                     VCSGGRYDDLAGSYINKHLPGVGISIGFTRLFDVLRQAGKIEPGLKSPADVLVVLPSE
                     ERRDEAAQAARTLRERGVKVELYHAPQKVGKQIAYAEKKGIPHVWFPPFEDGQAHEVK
                     NMSTGKQMQADPKEWNPDE"
     gene            1523..2197
                     /locus_tag="PK513_09965"
     CDS             1523..2197
                     /locus_tag="PK513_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82816.1"
                     /translation="MNRFLSLRTLVKQSRIWIAASAFALLAMTVMSALAQDEEAQTKK
                     EQPAEEPQGHTYSPDFCEFSVTFPSAPYAARRCEDEGKDRCYDLVSYTQVYEMSSTIN
                     FRIICNPIDSAVYDEYSAEVMQATLRAMTRKTVVEEYNSGFREGDGYKQAGLAGAGKV
                     GRTPMIYIAQLWIGKQSAFSVEAELIGEAHETADQAFSDILKTVRYSGVKDVEPPVKT
                     KAPERD"
     gene            complement(2194..2595)
                     /locus_tag="PK513_09970"
     CDS             complement(2194..2595)
                     /locus_tag="PK513_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOO82817.1"
                     /translation="MSQETLNELIEDLALFDDWEERYRYLIDLGRALPAMDVSLKNDE
                     SLVRGCTSRVWIVPEIRDGEFYFKADSDAHIVRGLIAILDKTYQGVKLSKLGAIDIES
                     AFAKMGLDQNLSPNRRNGFFSMVERIKSFAT"
     gene            2717..4021
                     /gene="clpX"
                     /locus_tag="PK513_09975"
     CDS             2717..4021
                     /gene="clpX"
                     /locus_tag="PK513_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOO82818.1"
                     /translation="MSKSSDKTDSKNTLYCSFCGKSQHEVRKLIAGPNVFICNECVEL
                     CMDIIREEDKTQLVREGENVPAPSEIKQVLDDYVIGQEAAKRVLSVAVHNHYKRLEHG
                     GTGSGSDVELAKSNILLVGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDV
                     ENIVLKLLQASDYNVERAQKGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLME
                     GTVASVPPQGGRKHPQQEFLQVDTANILFIAGGAFAGLDKVIAARGRGTTIGFGADVK
                     DLDERGIGELFKELEPEDLLKYGLIPEFVGRLPVIATLEDLDEDALITILTEPKNALV
                     KQYKKLFDIEGVELTFEDSALTAIAKKAIERKTGARGLRSILENLLLDTMFELPDIEG
                     LEEVIINKKTVEDGKDPVMVIADDKKKKKKPAGKKDKEAAKA"
BASE COUNT         1065 a          899 c         1161 g          967 t
ORIGIN      
        1 ccatgaccct ccttcggtca tagatcgtag cttaactcac tgtccgaatt cttggagcca
       61 cctctattcc atcgcgaatt aaagcttgtc ttcattgcag cgatcccgta agcaaagcga
      121 atggctaagc aaaataaaat ctataaaccc aaacccgtaa gtggttttcc tgaatggttg
      181 ccggaatacc gcgccgttga gcagtgctgg ctggataaaa tccgcgccgt gtttgaaagc
      241 tatgggtatt gttcgataga gacgcccgcc gttgaggaat tggaggtgat tgccgccaag
      301 ggcgaggatg tcgataaaga gatttacgtg ctggagcgct tgcaggccga cccggatgag
      361 aaaaaagatg cgcggctggc gctgcatttc gaccagacag tgccgctggc gcgctatgtc
      421 gcacagcatt ttgccgatct gacctttccg ttcaagcgtt accagatgca aaaagtctgg
      481 cgcggtgagc gcccgcagat gggccggatg cgtgaatttt atcagtgcga catcgatgtc
      541 atcaatgtcg attccctgcc gattcacttt gatgcagaag tcgcggcgat ctgttatgag
      601 gcgctcgaag cgctggatat gggtcgcgtg caattgcggg tgagtaatcg gaagattttg
      661 atggggtttt tggaaagctt aaatgttccg gataaaaaaa ctaacggcga aactacagtc
      721 aaactctcag atgaaaccaa agcagaaatt ataagagaca tcgacaagct tgagaaaatc
      781 ggaaaaaaag tcttgctatc aagactcgaa caaacccttt tggtggtcgg agagttaggg
      841 tttaattttc agacatttat agaacctttt ttgaatcttg cggaaactaa aataaagccc
      901 ggtgcaaata ttctggaatt gtttcaaaac aatagcgaga ttgattcaag taacgaaatg
      961 ctgcgtgaag ggattgaaga acttcaattt gtactggacc aactcgccca cctccctgaa
     1021 catgcggtgg ttgccgacct gtccatcgtg cgagggctcg attattacac cggcacggtc
     1081 tacgaaacgc aactgctcga tgtgccggaa tttgtcggtt cggtgtgcag cggcgggcgg
     1141 tatgacgatc tggcgggcag ttatatcaac aagcacttgc ccggcgtggg gatttcaatc
     1201 ggctttacgc ggctgtttga cgtactgcgt caggcgggca agattgaacc ggggctgaaa
     1261 tctccggcgg acgtgctggt ggttttacca tcggaagagc ggcgcgatga ggccgcgcag
     1321 gccgcgcgga ccctgcgtga acgtggagtg aaggtcgagc tgtatcacgc accgcaaaaa
     1381 gtcggcaagc aaatcgctta tgccgagaaa aaagggattc cgcatgtctg gtttccgccg
     1441 tttgaggatg gacaggcgca cgaagtgaaa aatatgtcca caggtaagca gatgcaggcc
     1501 gatccgaaag agtggaatcc tgatgaatag gtttttgtca ttgcgaacgc tagtgaagca
     1561 atccagaatc tggattgccg cgtcggcctt cgctctcctc gcaatgacgg tgatgtctgc
     1621 attggcgcaa gacgaagaag ctcaaactaa gaaagaacag cccgctgaag aaccgcaggg
     1681 gcatacatat tcccctgatt tttgtgaatt ttccgtgact tttcccagtg ccccttacgc
     1741 tgcgcggcgc tgtgaggatg agggtaaaga tagatgttat gatctggtga gctacacgca
     1801 ggtttacgag atgtcctcaa ccatcaactt tcggattata tgcaacccga ttgattctgc
     1861 cgtctatgac gaatacagcg ccgaggtgat gcaggcgacc ttgcgggcca tgacacgcaa
     1921 aacggttgtt gaggaatata atagcggttt tcgtgagggt gacggctata agcaggcagg
     1981 gcttgcaggc gcgggcaagg ttgggcgcac accgatgatt tatatcgcgc agctgtggat
     2041 tggcaaacaa tcggcctttt ccgttgaggc ggagttgatc ggcgaggcgc atgagacggc
     2101 ggatcaggcg tttagcgata tattgaaaac cgtgcgttac agcggcgtta aagatgttga
     2161 gccgccggtg aagaccaagg caccggagcg ggattaggtt gcaaaagatt taatccgttc
     2221 gaccatggag aagaagccgt tacggcgatt gggggagagg ttctggtcga ggcccatttt
     2281 ggcgaaggcg gattcgatgt cgatagcgcc aagttttgaa agctttacac cctgataggt
     2341 tttatccaga atcgcgatca gaccccggac gatgtgggcg tcgctgtcag ccttgaagta
     2401 aaattcacca tcgcgaattt ccgggacaat ccagacgcgc gaagtacagc cccggaccag
     2461 cgattcatcg tttttcagcg atacatccat ggcgggtaaa gcgcgcccga gatcaatgag
     2521 atagcggtag cgctcttccc aatcatcaaa aagtgcgaga tcttctatca gttcattcaa
     2581 ggtttcctga ctcatgagca gttgtataac atttattttc gggagaaaaa caacaaattc
     2641 attatataag gcttgcgcgc ctgccttcgc gttcgctaga ttgggagagt aacataaaga
     2701 atggacccct ctttccatga gcaaatcttc agataaaacc gatagcaaaa ataccctgta
     2761 ttgttctttt tgcggcaaaa gccagcatga ggtgcgtaag ctgattgctg gcccgaatgt
     2821 tttcatttgc aatgaatgcg ttgagctgtg catggatatt atccgtgaag aggataagac
     2881 acagcttgtt cgcgaagggg aaaatgttcc tgccccgtct gagatcaaac aggttttgga
     2941 tgattatgta attggccagg aagctgccaa gcgcgtgtta tctgtggcgg tgcataacca
     3001 ttacaagcgt ctggagcatg gcggaactgg ctcaggcagc gatgtcgagt tggcgaagtc
     3061 aaacatcttg ctggttggtc ctaccggttg cggtaaaaca cttttggcgc aaacactcgc
     3121 gcgtattttg gatgtacctt ttacgatggc cgatgcgacc accctgaccg aagcaggcta
     3181 tgtgggtgaa gatgtggaaa atattgtgct caaattgcta caagccagtg attacaatgt
     3241 tgagcgcgcg caaaaaggga ttgtgtatat tgatgagatc gacaaaattt cgcgcaagtc
     3301 tgataacccg tcgattaccc gcgatgtgtc tggagaaggt gtgcagcagg ccttgctaaa
     3361 actcatggaa ggcacggtgg cctctgtgcc gccgcagggc gggcgcaagc acccgcagca
     3421 ggaatttttg caggttgata cggccaacat cctgtttatt gccgggggtg cgtttgcggg
     3481 ccttgacaaa gtgatcgcgg cgcgggggcg aggcacaaca attgggtttg gcgcggatgt
     3541 caaagatttg gatgagcgcg gcattggcga gctatttaag gaactggagc cagaagatct
     3601 gctcaaatac gggctcattc ctgaatttgt tgggcgtttg ccggtgattg cgacgctgga
     3661 agatctggat gaagatgcac tgatcaccat tttaaccgaa ccgaaaaatg cactggtcaa
     3721 gcaatataag aagctgtttg acattgaagg tgttgagctt accttcgaag attccgcttt
     3781 aacggcaatc gccaagaagg ccattgaacg caaaaccggg gcgcggggtt tgcgctccat
     3841 tttagagaat ctgttgctgg atacgatgtt tgagcttcct gatattgagg ggctggaaga
     3901 ggttatcatc aacaagaaga cggtagagga tggcaaagac ccggtcatgg tgatcgctga
     3961 cgataagaag aaaaagaaaa aaccggccgg taaaaaggac aaagaagcgg cgaaggctta
     4021 agccccagta tgcggcctta tgtgtaaaag tcataactta atctgacgat tatgaattat
     4081 cggccagcca gc
//
LOCUS       DAOSWR010000171         3895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_16243_length_3895_cov_5.226562, whole genome shotgun sequence.
ACCESSION   DAOSWR010000171 DAOSWR010000000
VERSION     DAOSWR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16243_length_3895_cov_5.226562"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="dgt"
                     /locus_tag="PK513_09980"
     CDS             complement(<1..769)
                     /gene="dgt"
                     /locus_tag="PK513_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOO82819.1"
                     /translation="MNSPNPQPSYNYCALPLAAYACVPEQSRGRVHEEKDSAHRSAFQ
                     RDRDRIIHASAFRRLKYKTQVFVYDEGDHYRTRLTHTLEVAQITRSIARALMVNEDLA
                     EAIALAHDLGHTPFAHIGEEYLAKCMAPYGGFEHNDQSLRVLTTLERKYPRWVGLNLT
                     WETLEGVVKHNGPHDMSPSGADIAVRELQEQIDLGLDSFASIEAQVAALSDDIAYNNH
                     DVEDGLRAHLFSLKDLEEVRLLGEVIAGVRDEYGDLNS"
     gene            799..1116
                     /locus_tag="PK513_09985"
     CDS             799..1116
                     /locus_tag="PK513_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006925186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOO82820.1"
                     /translation="MSLNIDESAIKRIEHLRTTKDQPGLCLRITVEGGGCSGFQYIME
                     LTESAKSKDLTFADCIVTDDISLPFLEGATIKFKDDLIGSEFIIDNPNAQSGCGCGSS
                     FSV"
     gene            1215..1979
                     /gene="xth"
                     /locus_tag="PK513_09990"
     CDS             1215..1979
                     /gene="xth"
                     /locus_tag="PK513_09990"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOO82821.1"
                     /translation="MKIASWNVNSLKARRAHVENYLASSGLDVLMVQELKGLDFPHED
                     FEKLGWHTAAVTQKTYNGVAIFSKTPVKVIKDALPGDKDDEQARYLEFETGGIRLIDI
                     YLPNGNPVGTEKFTYKLDWIERLYKRLKTLREDNIPFAIGGDFNVIPEDKDCYDPKKW
                     MDDALFQPESRAAFRKLLHLGLTEAFRAMHNGAGHYTFWDYQAGAWPKNHGIRIDHFL
                     LSPELADRLINCTIDKALRALDKPSDHTPIIIEIES"
     gene            1976..2596
                     /locus_tag="PK513_09995"
     CDS             1976..2596
                     /locus_tag="PK513_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82822.1"
                     /translation="MKHLLAFFSLLLLIACTTDESTYQHYLSNKGLESQLPERFQHCH
                     GYGCKYITPVRFTQSEWSEIEALFTPPLQSPEAERERIKTAIGLFEQKVGAKTGTSVD
                     IHGTFFKTGKYQLDCVDESTNTTVYLSLLQQKGLIRFHRVEGPSVRLPLIHAGRWPHQ
                     TAVITELNTSEFFAVDSWFHDNGAPAEIISLKEWKEGWKPNADSDS"
     gene            2637..3527
                     /locus_tag="PK513_10000"
     CDS             2637..3527
                     /locus_tag="PK513_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82823.1"
                     /translation="MNNFLKLSVLALTGISALGLNACGPHEPFREQVAARLASPAWMI
                     KRPVESGPFIITAFERMHERGKSANIYIEGAGEADETMIKGVLFNPTPRNPVALHLAS
                     MDKADNVAYLARPCQYSGLRDPESACDESYWGDAQFNAETVTSYHSVLDNIKARYGVT
                     EFNIVGYNSGATLAALLAGQRKDVKSLRTVAGHFDIVALSGAMESLKNVPQHHFIGGQ
                     DETAPPAYLENYLQALGDTPCVDYTFIQEAAHEKGWVNKWPELLKNDVPECYIPPEPE
                     FIPIEKPEPIYVPRMGGSKK"
     gene            complement(3815..3889)
                     /locus_tag="PK513_10005"
     tRNA            complement(3815..3889)
                     /locus_tag="PK513_10005"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3855..3857),aa:Phe,seq:gaa)
BASE COUNT         1051 a         1173 c          836 g          835 t
ORIGIN      
        1 ggctgttcaa atcaccatat tcatcgcgca caccggcaat cacttctcca agcaaacgca
       61 cctcttcaag atccttgagg ctgaacaaat gcgcgcgcag cccatcctca acgtcatgat
      121 tattgtaagc aatatcatcg gaaagcgccg ccacctgcgc ctcaatgctg gcaaaactat
      181 ccagccccag atcaatctgc tcctgcaact cgcgcactgc aatatcagct ccgctgggtg
      241 acatatcatg cggcccgtta tgcttcacca ccccttccag cgtttcccaa gtcagattga
      301 gcccaaccca gcgcggatat ttccgctcca gagtcgtcaa cacgcgcaag gactggtcgt
      361 tatgctcaaa cccgccatag ggcgccatgc acttggccag atactcttca ccgatatgag
      421 caaacggcgt atggcccaga tcatgcgcca gcgcgatcgc ctcggccaga tcctcattca
      481 ccatcagcgc ccgcgcaatc gagcgtgtaa tctgcgccac ctccagcgta tgggtaagcc
      541 gtgtgcggta atgatccccc tcatcataga caaacacttg cgttttatat ttcaaccgcc
      601 gaaacgcact ggcatgaata atccggtccc ggtcccgttg aaacgcgctg cgatgcgcac
      661 tgtccttttc ctcatgcacc cggccacgag attgttccgg cacgcacgca tacgccgcca
      721 acggcaacgc acaataatta taggaaggtt gtgggtttgg actgttcatc tgaaactcta
      781 acgctatata atagagttat gagcttaaac attgacgaaa gcgccatcaa gcgcattgaa
      841 cacttacgca caaccaaaga ccaaccgggc ctttgcctac gcatcacggt tgagggcggc
      901 gggtgttcag gctttcaata catcatggaa ctcaccgaaa gcgcgaaaag caaagaccta
      961 acctttgccg attgcatcgt caccgacgac atttccctgc ccttcctcga aggtgcaacc
     1021 atcaaattca aagacgacct aatcggctct gaattcatca tcgataaccc caatgcccaa
     1081 tccggttgcg gttgcggtag ttctttttcg gtataaccaa caccgtcatc acaacacaaa
     1141 gattgcatgg acaagccatg caataacgaa gaatctgtgt tgatctgtgt tcatccgtgg
     1201 ttaaacttcc ttccatgaag atagcctcat ggaatgtgaa ctctctgaaa gctcgccgcg
     1261 cgcatgttga aaactatctt gcaagcagcg ggctggatgt cctgatggtg caggaactca
     1321 aaggcctcga cttcccacat gaggactttg aaaagctcgg ctggcatact gccgccgtca
     1381 cacagaaaac ctataacggc gtcgccatct tctccaaaac cccggttaag gtgatcaaag
     1441 acgccctgcc cggcgataaa gacgatgaac aagcccgcta tctcgaattt gaaaccggcg
     1501 gcatacgcct gatcgatata tacctgccca acggcaaccc cgtcggtacc gaaaaattca
     1561 cctataaact cgactggata gagcgcctgt ataaacgcct gaaaaccctg cgcgaagaca
     1621 acatcccctt tgccattggc ggcgatttca acgtcatccc agaagacaaa gattgttacg
     1681 atcctaaaaa atggatggac gatgccctct tccagcctga atcccgcgcc gccttccgca
     1741 aactcctcca tctcggcctg acagaagcct tccgcgccat gcataacggc gccggacact
     1801 ataccttctg ggactaccag gccggggcat ggcccaaaaa ccacggcatc cgcatcgatc
     1861 attttttact ctcccccgag cttgccgatc gcctgataaa ctgtacaatt gataaagcgc
     1921 tgcgcgcact tgacaaaccc tctgaccata cacccatcat tatcgaaatt gaatcatgaa
     1981 acacctgctg gcatttttca gcctcctgct cttaattgct tgcaccaccg atgaatcaac
     2041 ctatcagcat tatttgagca acaaaggcct tgagagccaa ctccccgaac gtttccagca
     2101 ttgccacggc tatggctgca aatacatcac gcccgtccga ttcacccaaa gcgaatggag
     2161 cgaaatcgaa gccctcttta cgccccccct acaatcgccg gaagccgaac gcgagcgcat
     2221 aaaaaccgcc atcggcctgt tcgaacaaaa agttggagct aaaaccggca ccagcgttga
     2281 cattcatggc accttcttca aaaccggtaa ataccaactc gattgtgtcg atgaaagcac
     2341 caacaccacc gtatatctat ctctactcca gcaaaaaggc ctgatacgct tccaccgcgt
     2401 cgaaggccca agcgtccgcc tgcctctcat tcatgccgga cgctggccac accaaaccgc
     2461 cgtcatcacc gaattaaaca ccagcgaatt cttcgccgtc gattcctggt ttcacgataa
     2521 tggcgcccct gctgaaatta tttcactaaa agagtggaaa gagggttgga aaccaaatgc
     2581 agacagtgat tcttaaaatt gactatgcta gagtattcaa aaatacgggg tatctcatga
     2641 ataattttct caaactctca gttttggcct taactggaat cagcgccctc ggcctcaatg
     2701 cctgcggccc gcacgaacct ttccgcgaac aggtcgccgc gcgcctggcc agcccggcat
     2761 ggatgataaa gcgccccgtt gaatcaggcc cattcatcat caccgccttt gaacgtatgc
     2821 atgaacgtgg caaatctgcc aacatttaca ttgaaggcgc aggcgaggcc gatgaaacga
     2881 tgatcaaagg cgtactattc aaccccacac cgcgcaaccc tgtagccctg cacctcgcct
     2941 caatggataa agccgataac gtcgcctatc tcgcccgccc atgccagtat tcaggcctgc
     3001 gcgatccaga aagcgcctgc gatgaaagct attggggcga tgcccaattc aacgccgaaa
     3061 ccgtaacctc ttatcacagc gtcctcgaca atattaaggc ccgatacggc gtcaccgaat
     3121 tcaacattgt cggctataac tccggcgcca cactggccgc actgctggcc ggacagcgca
     3181 aagacgtaaa atccctgcgc accgttgccg gacattttga catagtcgcc ctttcaggcg
     3241 ccatggaatc cctcaaaaat gtgccgcaac atcacttcat tggtggccag gacgaaaccg
     3301 ccccgcccgc atatttggaa aactacctgc aagctttagg tgacacccct tgcgttgatt
     3361 acacctttat ccaagaagct gcacatgaaa aaggctgggt caacaaatgg ccggagcttt
     3421 tgaaaaacga tgttcctgaa tgctacatcc cgcccgaacc ggaattcatc ccgatcgaaa
     3481 aaccggagcc catttacgtt ccacgcatgg gtggttccaa gaaataaatt tgaaaaaatg
     3541 aataaccctg ccgcaagagg ctaggtatcc tatcattgca agcctccgaa gacggtgaag
     3601 caatccagac aggatagggt ttttgcgggt ttgaccggga tagtttttgt agcaaatgta
     3661 gcaaatacag taaatatcaa agcaatatag atacccacgg gtaacggaaa caacataccg
     3721 cctccgggct cattcatcca cgggtaaacc cgtggtgttc tgtcctacgg accgcataaa
     3781 aacaaaattt ccactaactc attgattttt aaaatggtgc ccgggggtag aattgaacta
     3841 ccgacacggg gattttcaat cccctgctct accactgagc tacccgggca tgttt
//
LOCUS       DAOSWR010000172         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_16540_length_3849_cov_4.797839, whole genome shotgun sequence.
ACCESSION   DAOSWR010000172 DAOSWR010000000
VERSION     DAOSWR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16540_length_3849_cov_4.797839"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..624
                     /locus_tag="PK513_10010"
     CDS             55..624
                     /locus_tag="PK513_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOO82824.1"
                     /translation="MNAATSLEQCPALVLNADYRPLSYFPLSIWPWQEAVKAIFRDTV
                     TVISEYDRVVHSPSFDMKLPSVLALKEYVPRARSPAFTRFNVFLRDRWQCQYCANTFK
                     THELTFDHVIPRSRGGRTNWENIVAACQKCNTQKGNRMPHEVNMYPIHEPRQPMLHEL
                     QGHGRKFPPNFLHKSWGDFLYWDTELDEL"
     gene            768..1121
                     /locus_tag="PK513_10015"
     CDS             768..1121
                     /locus_tag="PK513_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82825.1"
                     /translation="MFNHLTNLAHTRSITGAVGFFLFHVMLLVGLSTFLGHYLVTLGV
                     IEGSVGSFFDGGSIHTMIGAIWTLVLGSTILTGKKLTGDIMSVVIMFLGVYLAYTVNV
                     MLGMVVVSYLTTLGK"
     gene            complement(1308..1889)
                     /locus_tag="PK513_10020"
     CDS             complement(1308..1889)
                     /locus_tag="PK513_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOO82826.1"
                     /translation="MMVSFIRKLLSCRVLVLLGITGVSVFSLLMALTAEVYFGLEPCI
                     LCVYQRFPFLAVAILGLIGLAMKSKPAVQRVIIALSGLTFLVNSIIASYHTGVEQKWW
                     ASAFEGCAVPPLGDEPQTILENILSAPTANCAEIPWQDPIIGLSMANYNALMCFGLFV
                     VCILSFMLLRNTQKTEGCHHAQHQCGMENKETD"
     gene            complement(1886..2512)
                     /locus_tag="PK513_10025"
     CDS             complement(1886..2512)
                     /locus_tag="PK513_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HOO82827.1"
                     /translation="MNEQPRKISKVLIDLQKTLPEEKICTFDLLEALHERGFGFLLFI
                     FALPAALPLPGLGVNVIIALPLLFLTAQQALGRHSIWIPEKMKYKSISRARFEAMLTA
                     GLPFITKVELLVRPRLGFITHGLFGNLIGVAGLVMALSICVPLPLTNTVPAMGIALMA
                     LGVIMRDGLAVLTGAILGLIWVAMLAYVLIFLGTEGLDLIKETIRSLI"
     gene            2767..>3849
                     /locus_tag="PK513_10030"
     CDS             2767..>3849
                     /locus_tag="PK513_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOO82828.1"
                     /translation="MAKHTLMGGKLHVYKRENSSYWQCSAYLGGRNHRTSTKEESLPH
                     AKDFAEGWYLDLKCKDLRGDLRNEKTFKDAAERFIQEYEIITLGERNPEYVETQRRRI
                     ETHLMTFFGKMGLSEITSGKVQEYRIWRRETSMDKYGKVPARSTMHQEVVALRQTLKL
                     AVRNGWLDRLPDISEPYKASGKITHRGWFSPDEYKKLYEATRRRAKKPTRERYAWESE
                     QLHDFVLFMANTGLRPDEAKRLEYRDVEIVEDWDTGETILEIEVRGKRGVGYCKSTKG
                     AVEPFKRLVERNKPKPTDRVFPKLHRELFNTILKEEDLKVDREGNNRTAYSLRHTYIC
                     LRLMEGADIYQIAKNCRTSVEMIEKYY"
BASE COUNT         1085 a          914 c          947 g          903 t
ORIGIN      
        1 actatcggac ctccgaccgt tgccaacggt aaaaaacgtt aggagtgtgg gcacatgaat
       61 gcagcaacct ctcttgaaca atgtccagcc cttgtgctga atgctgacta taggccgctg
      121 agttattttc cgctttcaat atggccttgg caggaagctg tgaaggccat ttttcgagat
      181 acggtcacgg tgatatcgga atatgatcgt gttgtccact caccgagttt tgatatgaag
      241 ctgcccagtg tgctggctct gaaagaatat gtgccgaggg cgcgaagccc ggcctttaca
      301 agatttaatg tgttcctgcg tgatcgctgg caatgccaat attgtgcaaa cacatttaaa
      361 acgcatgaac taacttttga tcatgtaatt ccgcgctcca gaggagggcg tacgaactgg
      421 gagaatatcg ttgcggcatg tcagaaatgc aacacgcaaa aaggcaaccg catgccgcac
      481 gaagtaaata tgtatccgat ccatgagcca aggcagccta tgctgcatga gcttcagggg
      541 cacgggcgaa agttcccccc gaactttcta cacaaaagct ggggagactt tctgtactgg
      601 gatacggaat tagacgaact ctaatagggt tcaaaataaa gtttcggctt tgtcacggag
      661 aacggtttag aaaataaagc tcctttgaca ctgctggaaa agacgcggac aaggccacgg
      721 ttggacttat gtttccgctt aaccacgtgc aacgaaagga gtgtattatg tttaatcatc
      781 ttacaaatct tgcacatact cgttcgatca caggtgcagt agggtttttc ctattccacg
      841 ttatgctgct ggtcggatta tcaactttcc tgggtcacta tctggtgaca ctgggtgtga
      901 ttgaaggttc tgtcgggagt ttcttcgacg gaggctcgat ccataccatg atcggtgcga
      961 tctggacttt ggtcctgggc agcacaatcc tgacaggaaa gaaactgaca ggcgacatca
     1021 tgtctgtagt gatcatgttc ctcggcgtgt atctagcata caccgtgaac gtgatgctcg
     1081 gtatggtcgt tgtgtcttac ctgaccacat tgggtaagta acagaactaa aatattgaaa
     1141 gagccggggt ggcgtaaagc gccccggctt ttttgttcct cttaaaaacc ttgactttcc
     1201 gcggtctttc gcgctatgaa cctttgtttg cagcattctg agtaaaaggt tctaaggtca
     1261 tgtttgacga tttatttgat ttttccaaga aacgcacact gaagcaatca gtcggtttct
     1321 ttattttcca ttccacattg gtgctgggcg tgatggcagc cttcagtctt ctgggtgttt
     1381 cttaaaagca taaagctcaa aatgcatacc acaaacagcc cgaagcacat cagcgcattg
     1441 taattcgcca tggataggcc gatgatcgga tcttgccacg gaatttctgc gcagttcgcc
     1501 gttggtgcgc tcaggatgtt ctctaaaata gtctgcggtt catcccccag aggcgggaca
     1561 gcgcagccct caaaggccga agcccaccat ttctgctcca cgcctgtatg gtaagaggcg
     1621 ataatgctgt tgaccagaaa ggtcaggccg ctaagggcga tgatgacccg ctgcaccgca
     1681 ggcttcgatt tcatcgccag tccaataaga cctaaaatgg ctacagccag aaatggaaaa
     1741 cgctggtaaa cacaaagaat gcaaggctcc agcccgaaat acacttctgc cgtcagcgcc
     1801 atcaacaagg aaaataccga aaccccggta atgccaagca ggacaagcac gcggcaggac
     1861 agcaatttgc gaataaacga aaccatcata ttaatgatct aatcgtttct ttgattaaat
     1921 ccaacccttc cgtacctaaa aatataagaa cataagccag catagcaacc catatcaggc
     1981 ccagaatcgc tccggttagc accgccagcc cgtcgcgcat aatcacaccc agcgccatca
     2041 gggcaattcc catcgcgggc accgtattgg tcagaggcaa cggcacacag attgacagcg
     2101 ccatgacaag cccggccacc ccgatgaggt tgccaaacaa accgtgcgtg ataaagccca
     2161 gccgcgggcg aaccaaaagc tcgactttgg tgatgaaggg caggccagcc gtaagcatag
     2221 cctcaaaccg tgcccggctg atggacttat atttcatctt ctccgggatc cagatcgaat
     2281 gccgtcccag cgcctgctgc gctgtcagga acaacagcgg cagcgcgata atcacattca
     2341 cgcccagccc cggcaacggc agcgccgcgg gcagtgcaaa gataaacagc aaaaatccaa
     2401 aaccgcgctc gtgcagggct tcgagcaagt caaatgtaca aattttttct tctggtaaag
     2461 ttttttgaag atcaattaaa actttggaga ttttgcgcgg ctgttcattc atccgaacaa
     2521 aataacgaaa ttggtattga aataaaacca ataaacgctt attgataacc gctattcgat
     2581 gtggtgcgcc tctgtggcgg aactggtaga cgcgcgggat tcaaaatcct tacttatact
     2641 tccctttcaa aattaagttt tctttaaaaa cagtctgtta agcttgtttt taggccagat
     2701 ttcttggttt tgcacactta cacaactctt acacaaaatg acacagtgtg tcgggaaggt
     2761 acgaaaatgg cgaaacatac gctaatggga gggaaactcc acgtttacaa acgcgaaaac
     2821 agcagttact ggcagtgctc agcttacctc ggaggaagga atcataggac cagcaccaag
     2881 gaagaaagtc taccacatgc taaggacttt gccgagggct ggtatctcga tcttaaatgt
     2941 aaagatttac gcggtgactt aaggaatgaa aaaacattca aagacgccgc agagcgattt
     3001 attcaggaat atgagattat cacgctgggt gaaagaaacc cggaatacgt tgagacgcag
     3061 cgtcggcgta ttgaaacgca ccttatgact tttttcggta aaatggggct gtctgaaatc
     3121 acctcgggca aggtgcagga atatcgcatc tggcgcaggg aaacctcaat ggataaatac
     3181 ggtaaggttc cggccagaag cacaatgcat caggaagttg ttgccttaag acaaacctta
     3241 aagctagcgg tccgaaacgg ttggctggat cgtttgcccg atatttccga accctacaaa
     3301 gcttcgggta aaattacgca tagaggctgg ttttcaccgg acgagtacaa aaaactctac
     3361 gaagccacgc gcaggcgggc gaaaaaaccc acgcgcgaac gctatgcgtg ggaatcagaa
     3421 cagttgcacg actttgttct gtttatggcc aatactggcc ttcgtcccga tgaggccaaa
     3481 cggctcgaat acagggacgt ggaaattgtc gaagattggg acaccggtga gactattctg
     3541 gaaatagagg tgcgcggaaa gcgcggtgtg ggatactgca aaagcaccaa aggggctgtt
     3601 gagcctttca agcgccttgt ggaaagaaat aaaccaaaac ctacggatag ggttttcccc
     3661 aagctgcaca gggagttgtt taacaccatc ttgaaggaag aagacttaaa ggtcgaccgt
     3721 gaaggcaata accgcacggc atatagcctc cgccatactt atatctgtct gcgcctgatg
     3781 gaaggggcgg atatttacca aattgctaaa aactgccgta caagcgtcga aatgattgag
     3841 aaatattat
//
LOCUS       DAOSWR010000173         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_17150_length_3757_cov_5.797137, whole genome shotgun sequence.
ACCESSION   DAOSWR010000173 DAOSWR010000000
VERSION     DAOSWR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17150_length_3757_cov_5.797137"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PK513_10035"
     CDS             complement(<1..1795)
                     /locus_tag="PK513_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO82829.1"
                     /translation="MIQFKRLRINGFKSFVDRTELDIGPGLNGVVGPNGCGKSNLVEA
                     LRWVMGENSARRMRGDGMEDVIFNGTSKRSARNIADVSLLLDNSSRVAPAAYNGADEI
                     EVVRRIERDRGSNYKINGKNVRARDVQMLFADTVTGANSPAMVSQGRITQIINAKPLE
                     RRLVLEESAGISGLFARRHEAELRLKAADTNLIRIEDILGSMEGRLGALKRQARQATR
                     YRNVSTQIRQMEILIAWMEWQRLDERMSATRRSFEEAESQVVARLSVVSALTKTQGTQ
                     SEDLPALRQAEAEIAAGLQTQKLALQRLEDESGRIDQLIEETKEQLEQAQTDKGHEEA
                     SLEESSGLLEKLEVEHSAIAAEQKSEDSKLEEKDAARAMLEAKVLELEERYNALMQGT
                     AEAKARKNALTQRIESNTRRKETLEARRDQAQSDLKGVRNAQPAKGRNFEKEITDQEK
                     AKEDYDQKIETLETDLETVAGDIDRAQEALNTAKNKKAEFDTEISVLYSVLKLEDEGD
                     FAPVLQDVSAAQGFEKALSRALGDSLMASLDEKASSTWLPVKSTAALPALPKGADELR
                     PHVKAPKVLNIALSQIGVVETAKDGDALWESL"
     gene            complement(1792..2676)
                     /locus_tag="PK513_10040"
     CDS             complement(1792..2676)
                     /locus_tag="PK513_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO82830.1"
                     /translation="MADKTSKTDKKKDKGATAKSSDTKIFIGVGILFLFLAIAYGMSL
                     GPIEIPEAPKANIEESEIDEIEQAYSEDGMAIDDAEEVVADDIEVEVSENLETSSPAF
                     DLAAAKKERVLGDPSAPIKITEHSSLTCGHCGKFHTETFKELKAAYLDTGEAYLVFSD
                     FPLNAPALHATMAARCIADNQKYFEFIGELFETQKDWAGENNYLSLLESKAEAYGIDK
                     ATFAACVQNQELQDSILKRMQAVQKQWDISSTPSFVIDNQVVLSGARPYDAFDKAIKD
                     AVTEIHGGASPEPTNEGE"
     gene            complement(2845..3585)
                     /locus_tag="PK513_10045"
     CDS             complement(2845..3585)
                     /locus_tag="PK513_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82831.1"
                     /translation="MVFRLVFFLFFMTLASPLFAFAQEGNVEINFETLENYSPPPMFE
                     QAVPDEVPARSEPVTKVSEPLARVVAPLPQHKPQHSGFIRQPNSQIDEGKLVIQTAQD
                     ILRAIDGDTSDVVPQPEANVVQTHSKPATTLKDLSSMSNDKAFEIVLPFEAYKTELSD
                     VQKRIVLQQVLIRLQKYDDATLEIRSYASSANNVESGARRTALARALVIEALLMDKGI
                     QETRIFLRPLGAVHGEQENAAVLSISRY"
BASE COUNT          860 a         1056 c          816 g         1025 t
ORIGIN      
        1 tgagcgattc ccacaaagca tcgccgtcct tggccgtttc caccacgcca atctgcgaca
       61 gcgcgatatt caaaactttc ggtgccttca catgcgggcg caattcatcc gcaccttttg
      121 gtaaggcggg cagggcggct gtgcttttca ccggcagcca ggtacttgaa gctttttcat
      181 ccagtgacgc catcaagctg tcgcctagcg cccgcgataa tgccttttca aaaccctgcg
      241 ccgccgaaac atcttgcagc acaggcgcaa aatcaccttc atcttctaat ttaagaacac
      301 tatacaacac actgatttca gtatcaaatt cagctttctt attctttgcc gtattgagcg
      361 cctcttgtgc ccggtcaata tcgcctgcca cagtttccag atctgtctcc agcgtctcaa
      421 ttttctggtc gtaatcttct tttgcctttt cctgatccgt aatctctttt tcaaaattcc
      481 ggcccttggc aggctgcgca ttgcgcaccc ctttcaaatc gctttgcgct tgatcacgcc
      541 gtgcctccag cgtctcttta cgccgtgtat tgctttcaat ccgctgcgtc aacgcgtttt
      601 ttcgtgcctt ggcttcggct gtgccctgca tcaaggcgtt atagcgctct tccagctcca
      661 ggactttagc ttccagcatc gcccgcgcgg catctttttc ttccagcttg ctatcctcgg
      721 acttctgctc agccgcaatc gcgctatgct ccacctccag cttttcaagc aggccgctgc
      781 tttcctctag tgaggcctct tcgtggccct tgtcggtttg cgcttgctcc aactgctctt
      841 tggtttcctc aatcaattga tcaattcggc cgctttcatc ttccagccgt tgcagggcaa
      901 gcttttgcgt ttgcaatcca gccgcaatct ctgcttcagc ctgacgcaac gccggcaaat
      961 cttccgattg cgtaccttgc gttttggtca gcgcactgac cacgcttaaa cgtgccacca
     1021 cctggctttc cgcctcttca aagctccggc gcgtggcgct catccgctca tccagccttt
     1081 gccactccat ccaggcgatc agaatttcca tctgacggat ttgcgttgaa acattgcggt
     1141 aacgtgtagc ctgccgggcc tgacgtttca atgcgcccag ccgcccctcc atactcccta
     1201 aaatatcttc aatgcggata aggtttgtat cggcagcttt aagccgcagc tcagcttcat
     1261 gtcggcgggc aaatagtccc gaaattccag cggattcttc caaaaccagc cgccgttcca
     1321 gcggctttgc attgataatc tgtgtgatcc gtccttgcga gaccatcgcc ggggaatttg
     1381 ctccggtcac tgtatcggca aacaacatct gcacatcacg ggctcgtacg ttcttgccgt
     1441 taattttata gttagacccg cggtcacgct caatccggcg tacaacttcg atctcatcgg
     1501 cgccgttata ggccgccggc gccacgcgcg aggaattatc cagcaggaga gaaacatcgg
     1561 cgatattgcg cgctgaacgt ttcgatgtgc cgttaaaaat cacatcttcc atcccatcgc
     1621 cgcgcatgcg ccgcgcagaa ttttctccca tcacccagcg caacgcttca acgagattag
     1681 actttccaca accattcggg ccaaccacgc cattaaggcc cggaccaata tcaagttcgg
     1741 tccggtcaac aaacgactta aagccgttaa tacgcagtcg cttgaattga atcattcgcc
     1801 ctcattcgtg ggttcgggag aagctcctcc atgaatttcg gtcacagcat ctttgatggc
     1861 tttatcaaaa gcatcatagg gccgtgcgcc actgagcacc acctgattat cgatcacaaa
     1921 gctcggggtg gaactgatat cccactgttt ttgtacagcc tgcatgcgtt tcaaaatgct
     1981 gtcctgcagc tcttgatttt gcacacaagc ggcaaaagta gctttgtcta tgccatatgc
     2041 ctccgccttg ctttcgagga ggctgagata attattttct ccagcccaat ctttctgcgt
     2101 ctcaaacagt tccccgataa attcaaaata cttttgatta tcagcaatgc accgcgccgc
     2161 catcgttgcg tgcagagcag gcgcgttcaa cggaaagtct gagaacacaa gataagcctc
     2221 gcccgtatca agataggcgg cttttagctc tttaaaggtt tcagtgtgaa atttcccgca
     2281 atgcccgcag gttagcgaac tatgttcggt aattttgatc ggtgcagacg gatcgcccaa
     2341 aacccgttct ttcttcgccg ccgccaaatc aaacgctggc gaagacgttt caaggttttc
     2401 ggagacctcc acttctatat cgtctgcgac aacctcttcg gcatcatcta tagccatgcc
     2461 atcttcgcta taggcctgct caatttcatc gatttcagat tcttcaatgt tggcctttgg
     2521 cgcttcggga atttcaatag gacctaagga catcccatag gcaatggcga ggaataaaaa
     2581 caatatcccc acaccaatga aaatcttcgt atcggacgac ttcgcagtcg cacctttatc
     2641 tttcttttta tccgttttag aagtcttgtc agccattggt cacggtcctt tataaaggtg
     2701 ttgttcacat aatgcatctg atcgtcagat gtcatagcag cctttgtttt tgaaatgcaa
     2761 ctaaaattcc cactctaaag cccatgatgt caatgtttct gcgggatcaa ggggggatta
     2821 tccacaagtg atagggaaga ggtttcaata tcgggagata ctcagaacag cagcattttc
     2881 ctgttcgccg tgcacagcac caagaggacg aagaaaaata cgtgtttctt gaatgccctt
     2941 atccatcagc agggcttcaa ttaccaacgc gcgcgcaaga gcagtccgtc gtgctccgct
     3001 ttcgacatta tttgcgctac tggcgtaaga gcgaatttca agagttgcat catcatattt
     3061 ttgtaaacgg atcaacacct gttgtaagac gatgcgtttc tgtacgtcgc tcaattcagt
     3121 tttataagct tcaaatggca gaacaatttc aaaagcctta tcattactca ttgagcttaa
     3181 atctttgagt gttgtagctg gcttagaatg tgtctgcacc acgttggctt ctggttgagg
     3241 cacaacatct gaggtatctc cgtcaatcgc gcgtaaaata tcctgagcgg tttgtataac
     3301 aagtttacct tcatcaattt gcgaattcgg ctggcgaata aaaccggaat gttggggttt
     3361 gtgctgaggt aaaggggcca ccacccgcgc cagtggttct gacactttgg taacaggctc
     3421 tgatcgggca gggacctcat caggcacagc ctgctcaaac attggcggtg ggctatagtt
     3481 ttccagtgtc tcaaaattaa tctcgacatt gccttcttgt gcaaaagcga aaagcggtga
     3541 agccaaagtc atgaaaaaca ggaaaaaaac cagtctaaac accattctgc ggcctcacgc
     3601 agcgcttttc agcacctttt tgatgtcact aaagatgcct tcattagacg ccagaatatt
     3661 accggtctcc aggggattgg ctttatgatc atccaattcc gcaataaaac ctccagcttc
     3721 cttgacgatc aagataccgg cggccacatc ccagggc
//
LOCUS       DAOSWR010000174         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_17195_length_3751_cov_4.352814, whole genome shotgun sequence.
ACCESSION   DAOSWR010000174 DAOSWR010000000
VERSION     DAOSWR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17195_length_3751_cov_4.352814"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PK513_10050"
     CDS             complement(<1..959)
                     /locus_tag="PK513_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOO82832.1"
                     /translation="MSFQAHIILGITLFGLTIFSLTVSAPAQNAQAVVAPWSVDNTDA
                     QSRIRQYQNFKQLQTRPLQELSANDTSYEYSPSALQKHANRRAQQPYNPLSQRLPEPR
                     PPLPSQTQTVLSALENMYSERIIDNLNQFGYDLFGVPNSNLRGVLENAAKSAPSIPSG
                     AVQDDFILAGGDELEIFFTGQRTDRGTYNVNSQGLLIIPDFPPIPAAGRSIGQVRISI
                     EAAAGNLHNTKPYVSLASVRQISVLVVGHVKKPGRRTLTVFHTILDALMEAGGIEKTG
                     SLRQIKLVRDGRSTQIDLYALLLYGSTHIDIQLRDGDRIIIPS"
     gene            complement(1182..2819)
                     /locus_tag="PK513_10055"
     CDS             complement(1182..2819)
                     /locus_tag="PK513_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOO82833.1"
                     /translation="MLLHIAGAVCLLLWGTRMVKLGFVRAYGTSLHRALLYSTRNRFY
                     ACASGIGVTSLLQSSTATALLLISFVKKSTIPLTAALAVLIGADIATTLVAQILTLNL
                     SWLSPALLILGVSGHIMHEHGGRKRHIFRIVIGLGLMLLALTLIKQASAPLTHSGTLP
                     LILAPLEFDPITAILFSAILTWVMHSSLAAVLLFASLASNNIIDLELGALLVLGANLG
                     GGFIPFIATYKDGVIARRITFGNIIMRTAILALTFFFMDTALGVVEIMSLDTARDLIN
                     LHMGFNLVLAFLFLPNVQWIARLCEKLLPEKTSLPPKAYTPLYLDNKALTTPVVALAG
                     AARETLRMAEMVQDMLEKTIEAFKQNDTTLAKAISEEDNTIDRLYYEIKLYMTKLTQE
                     ALDPKESDRYLQIITFATNLEHIGDIIDNSLMELAKKKIKKKERFSDKGWAEIKDFHR
                     SVVENMRMAQTIFLSEDPSLARNLIEQKRIVSDAAKSSSALHFERLRSGLPETIATSS
                     LHLDIIRDYRRINSYVTSVAYAILENAEKYKKQRKKR"
     gene            3580..>3751
                     /locus_tag="PK513_10060"
     rRNA            3580..>3751
                     /locus_tag="PK513_10060"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          951 a          707 c         1036 g         1057 t
ORIGIN      
        1 atggagggaa taatgatgcg gtcgccatcg cgcaattgta tatcgatatg ggtggagcca
       61 tagagcagca gggcgtagag gtcaatctgt gttgagcggc cgtcacgcac gagtttgatc
      121 tgccgcaagc ttccggtttt ttcgatgcca cctgcttcca tcaatgcgtc gagaatggtg
      181 tggaagacgg tcagggtccg gcgccctggt ttttttacgt gacctacaac cagcacacta
      241 atttggcgga cgctggccag tgaaacatag ggtttggtgt tgtggaggtt tccggctgcg
      301 gcttcgatgg agattcggac ttgaccgatt gagcgtccgg cggctggaat cggtgggaaa
      361 tccgggatga tgagcaggcc ttgtgagttt acgttgtagg ttccgcgatc ggtgcgctgg
      421 cccgtgaaga agatttcgag ttcatcgccg ccagcgagta taaaatcatc ttgcactgca
      481 ccggaaggga ttgaaggtgc cgatttagcc gcgttttcaa gaacaccgcg caggttacta
      541 tttgggacac cgaataaatc atagccaaac tgattcaaat tatcgatgat acgctcggaa
      601 tacatatttt cgagggcgga aaggactgtt tgcgtttgtg aagggagagg tggtctcggt
      661 tctgggaggc gttgggatag tgggttgtac ggttgttgtg cgcgtctatt tgcgtgtttt
      721 tgcagggctg acgggctgta ttcatagctg gtgtcatttg cgctcaattc ttgaaggggc
      781 cgggtctgga gctgtttaaa attttgatat tgccggatgc gactttgtgc gtccgtgtta
      841 tcaacgctcc atggggcgac gacggcctga gcgttttggg caggcgcact tacggtcagg
      901 ctaaatattg ttaagccaaa taacgtgatg cctaagatga tatgtgcctg aaaggacatg
      961 agccaagaat atgcccgtaa gaaattgaat ctattcaacc tttgtaagta tacgcataca
     1021 tatgcagtct tgtgaattcc tttcataggg ctatcccctt aaagttggca tttaggctcg
     1081 gtgacgctat acaggaggta tgagtattta tatgatcaag ctggttgtgg gcgtggagag
     1141 ccttgagggt tttgtaaaaa attgttttta gtgctggtta tttagcgttt ttttcgctgt
     1201 tttttgtatt tttcagcgtt ttccaaaatt gcataggcta cggatgtgac gtagctgttg
     1261 atgcggcgat aatcgcggat gatgtccaga tgtagcgaac tggtggcgat ggtttcaggc
     1321 aatcctgaac gcaagcgttc aaaatgcagg gctgaacttg atttggcagc atcgctgacg
     1381 atccgttttt gttcaattaa atttcgggcc agactagggt cttctgacaa aaagattgtt
     1441 tgagccatgc gcatattttc aacaacactg cggtgaaagt ccttaatctc ggcccatcct
     1501 ttatcggaaa aacgttcttt tttctttatt ttcttcttag ccagttccat caggctgttg
     1561 tcgataatgt cgccgatatg ttccaagtta gtggcaaagg taatgatttg aaggtagcga
     1621 tctgattctt tggggtcgag ggcttcttgc gtcagctttg tcatgtaaag tttaatctcg
     1681 tagtataggc gatcgatagt attgtcttct tcgctgattg ctttggctag tgtggtatcg
     1741 ttttgcttga aggcttcaat ggttttttcc agcatatctt ggaccatttc cgccatcctt
     1801 aatgtttcgc gtgcggcgcc ggccagggcg acgaccggag tggttagcgc cttattatca
     1861 agataaagcg gggtataagc ttttggtggc agagatgttt tttcaggaag aagcttctcg
     1921 cacagtctgg caatccattg cacgtttggt aagaaaagaa atgccagcac gagattaaat
     1981 cccatatgca gattgatgag atcgcgggct gtatcgagac tcataatttc aacaactccc
     2041 agagctgtgt ccataaagaa aaaggtcagc gccagtatag cggtgcgcat gataatgttt
     2101 ccaaatgtta tgcgtcgggc gataactcca tctttatagg tggctataaa gggaataaag
     2161 cctccaccga gatttgctcc cagtaccagc agcgctccaa gctctaagtc tatgatgttg
     2221 ttgctggcca atgaggcgaa caagaggact gcggccagtg aggagtgcat gacccatgtc
     2281 aatatggcgg aaaatagaat tgcagtgatt ggatcaaatt caaggggggc taaaattagg
     2341 ggcaatgtgc ctgagtgtgt tagtggggca gatgcctgtt tgatcagagt cagtgcgagc
     2401 agcatgagcc ccagtccaat cacaatacga aaaatgtggc gtttgcgccc gccatgttca
     2461 tgcataatgt gtccggaaac gcctaaaatc agaagagccg gggacaacca agataagtta
     2521 agggttagga tttgagctac gagcgttgtg gcaatgtccg cgccgataag aacagcgagt
     2581 gcggcagtca gtggaatggt gctttttttg acaaaggaga taaggagcag ggctgttgcc
     2641 gttgagcttt gcaagaggga tgttacacca ataccggaag cgcaggcgta aaagcgattt
     2701 cgtgtgctgt ataacaaggc tcgatgaagc gatgtgccgt aggcgcgcac aaagccgagc
     2761 ttgaccatac gtgtgcccca aagcaacaag caaacagcac ctgcaatgtg aagaagaatt
     2821 agagatacgg gaatgggatg actccttaaa gttttttatt tgagcgtcat ggaatatcca
     2881 gcgttagcgc gaaggcgttt gtctagtgta gcgtgtgtag aaataaaatc cattattata
     2941 taattgtttg ggagtatgtg ttttatttgc ccctatcttg tttaaactct taatattttg
     3001 ttgttggaag gtaagtgcag gggtgttttt tgttgttttt tcggggtgga cttaaaaaaa
     3061 ttaaaaaaag atgaaaaaaa ggtttgacgg gcgggagcga gatgcgtata tatccctcca
     3121 cgcctcgaga cgggggagcc tgaagcgcgg cgtgaagcac taaaaatcat agatttttga
     3181 ttgctgagtt gacgatctga ttatggcgga ttataagagg atcatcgctt gaaaagttca
     3241 gagacgaata acaagaagtt ctaaggcttc tttttattcg gttttgtgcc ggataggttc
     3301 ttgaacatcg tgaataggaa agaaggaata cgcaggcagc agtgctttgg cctcatttgt
     3361 gaggtgtgta ttgaatgcac ttaaataaag aagactgttg tttaatttaa gcgtatgtct
     3421 ttcatgacgc acgtgatcat ttatatgatc acaaacagtt tggttagagt gtacaagctt
     3481 taaccaagca gcaaattaag caggtctttg aagaagtcgt cgtctttatg atgacgcatt
     3541 acttcaatac caaaaattct tttgggcatt gatggattca aacttaagag tttgatcctg
     3601 gctcagaacg aacgctggcg gcaggcctaa cacatgcaag tcgaacgatc cttcgggata
     3661 gtggcgcacg ggtgcgtaac gcgtgggaat gtacacctcg gtaaggaata actgctggaa
     3721 acggtagcta ataccttata atgactccgg t
//
LOCUS       DAOSWR010000175         3630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_18060_length_3630_cov_5.327273, whole genome shotgun sequence.
ACCESSION   DAOSWR010000175 DAOSWR010000000
VERSION     DAOSWR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_3630_cov_5.327273"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..720)
                     /locus_tag="PK513_10065"
     CDS             complement(61..720)
                     /locus_tag="PK513_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOO82834.1"
                     /translation="MNPQIKKRLVRTALFSLIALVIGAGVGWVQIQMQAARVTTKDGK
                     QLDTIMPVAGLNIGGPFTLSDHTGAAVSEKTYAGKYKLIYFGFTYCPAICPTELQKMS
                     RIISALEKNHPEALENVQPLFISVDPERDTVPVMREYVGLFHPRLVGLTGTTPQVDLV
                     KKRYRVFASKVEDETNQDYTVDHSSFTYLMGPNDELIGMYRMKDTADQIYQDLAKRLG
                     L"
     gene            complement(775..1323)
                     /locus_tag="PK513_10070"
     CDS             complement(775..1323)
                     /locus_tag="PK513_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82835.1"
                     /translation="MQLMAESGDTRYQCYFMETDDLNDPAGLEMMLDARRLDFPYAIS
                     EGTRKAGIDILRATLNDPDQASFILWDSQNKEVVGRSIITYLSVSADNTPEITGLFVK
                     PALQGQHLVDVLYEGQIFALSEAGYGAAKVIISRENMRSVNAATRNGFYPIPSDASGG
                     LYLKKTIKQSPGVANALNIQSA"
     regulatory      1417..1523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1518..1862
                     /locus_tag="PK513_10075"
     CDS             1518..1862
                     /locus_tag="PK513_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HOO82836.1"
                     /translation="MPHIIVEYTDTLSSSEDIPKLLAKLHKDLASRESIDIHGIKTRA
                     IPVRYTVVGDGHEPDKMIHITLRLLPGRADDLKKQMAAGLVEVAREVTHHDDRISITA
                     EVVDMHAESYVK"
     gene            1948..>3630
                     /gene="thiC"
                     /locus_tag="PK513_10080"
     CDS             1948..>3630
                     /gene="thiC"
                     /locus_tag="PK513_10080"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOO82837.1"
                     /translation="MSPKDGPNKDGICPSTTHVTRGSLPASKKVYIGEHKVAMREILL
                     TNDDKITVYDTSGPYTDEAKAGNIDIMVGLCKIREGWIRGRGDVEEVEGRNVQPIDNG
                     YKDQTALDRRKVKHERFPASPEKPLRAKPGQNVTQMHYARKGIITPEMEYIAIRENQA
                     REQVHNNKGGEGFGANLPDFITPEFVRKEVAEGRAIIPANINHPESEPMIIGRNFLVK
                     INANIGNSAVTSSIGEEVDKMVWATRWGADTIMDLSTGKDIHDTREWILRNSPVPLGT
                     VPIYQALEKVGGIAEDLTFDIFADTLEEQAQQGVDYFTIHAGVRLPFIQYTADRITGI
                     VSRGGSIMAKWCMAHHRESFLYTKFDEICEIMKAYDVSFSLGDGLRPGAIKDANDRAQ
                     FAELKTLGELTRIAWDHDVQVMIEGPGHVPMHLIKENMEKELAECHEAPFYTLGPLTT
                     DIAPGYDHITSAIGAAQIGWYGTAMLCYVTPKEHLGLPNRDDVKTGVITYKIAAHAAD
                     LAKGHPGAQRRDDALSLARFEFRWNDQFNLSLDPETARSMHDETLPAEGHKVA"
BASE COUNT          995 a          978 c          854 g          803 t
ORIGIN      
        1 tcgcttcgct cgcgatgacg attacgccat aaacggcgag gaattaaacc cgaaaagaga
       61 ttaaagcccc aaacgcttgg cgagatcttg ataaatctga tcagccgtat ctttcatccg
      121 gtacatgccg ataagctcat cgttcggtcc catcagatag gtaaaggacg aatgatcgac
      181 cgtgtaatcc tgattggttt cgtcttcaac cttgcttgca aaaacgcggt agcgtttttt
      241 gacaagatct acctgcggcg tggttccggt caatcccacc aatcgtggat gaaatagccc
      301 gacatattct cgcatgaccg gaaccgtatc gcgctccgga tcaacagaaa taaagagtgg
      361 ttgcacattc tccagcgcct ccggatggtt tttctccaac gcgctgatga tgcggctcat
      421 tttttgtagt tccgtcgggc aaatcgccgg gcagtatgta aagccgaaat aaatcagttt
      481 atattttccc gcataagttt tctcgctaac cgcagcgcct gtatggtcgc ttaaggtaaa
      541 cggcccgccg atattcagcc ctgccaccgg cataatagta tctagttgct taccgtcttt
      601 cgtggtcaca cgcgccgctt gcatctgaat ctgcacccac ccaaccccgg ctccaataac
      661 aagagcaatc aggctaaaca atgccgtacg caccaagcgc ttttttattt gagggttcat
      721 agaaagagaa aatagttctt cgccgcgtta aaaacaacca ggaaattata acgattacgc
      781 agattgaata ttcaaagcat tagctacgcc gggtgattgt ttgatagtct tttttaaata
      841 caaaccgcca gaagcatctg atggaatagg ataaaaacca ttgcgagtcg cggcattcac
      901 agaacgcata ttttctctgg aaataataac tttagctgcg ccatatcctg cctcgcttaa
      961 tgcaaagatc tgcccttcgt aaagaacatc aacgagatgt tgcccctgaa gggctggttt
     1021 tacaaaaaga ccggtgatct caggagtgtt atctgcgctt acagataaat atgtaattat
     1081 actgcgccca acaacttctt tgttttggct atcccataaa ataaagctgg cttgatccgg
     1141 atcattcaaa gtcgccctta aaatatcaat tcccgctttt cgcgttcctt cagaaattgc
     1201 ataaggaaaa tcaagacgtc tggcatcaag catcatctca aggccggcag gatcattaag
     1261 atcatccgtt tccatgaaat agcattgata gcgtgtatct ccactttcag ccattaattg
     1321 catagaatgg cttatagcat aagttataca taacggcaaa tattgtgcct taaacaacat
     1381 tgacaaattt cataaatctg ccaacttcta gggcattaac aaccttcggg gtgcctcgtc
     1441 gaagccttgg caaagacggg ctgagaaaga cccgtagaac ctgttcggct aataccggcg
     1501 tagggaaagg aaaacacatg ccgcacatca ttgttgaata tacagacacg ctttcttcat
     1561 ccgaagacat ccccaagctt ctggctaaac tgcacaaaga cctggcctct cgcgagagta
     1621 tcgacatcca cggtatcaaa acccgcgcga ttcctgtgcg ctataccgtt gttggtgacg
     1681 gccatgagcc cgacaagatg attcacatca cgctgcgcct tttgccgggc cgggccgatg
     1741 atctcaaaaa acaaatggct gccggtctgg ttgaagtcgc gcgtgaagtt actcatcacg
     1801 atgaccgtat ttcgattact gccgaggtcg tcgatatgca tgctgaaagc tatgtgaaat
     1861 aaggaatgat aaataagcag cggagtatcc gtatccttta aacagttcac attatgtaaa
     1921 ataatataga aggaaagaac tctaaaaatg tcccccaaag atggcccaaa caaagatggc
     1981 atatgcccat ccaccaccca tgtcactcgc ggaagcctgc ccgcctccaa aaaggtctat
     2041 attggcgagc ataaagttgc catgcgcgaa attttgctca ccaacgatga caagatcacc
     2101 gtttacgaca cctccggccc ttacacggac gaagccaagg cagggaatat tgatattatg
     2161 gtcggattat gtaaaatacg tgaggggtgg attagaggca gaggcgatgt tgaagaggtt
     2221 gaaggccgga acgtccagcc catcgataac ggctacaagg atcagaccgc cctcgatcgc
     2281 cgcaaagtga agcacgagcg cttccccgcg tcccctgaaa aacccctgcg agcaaagccg
     2341 ggccagaatg tcacccaaat gcactacgcc cgcaaaggca tcatcacccc cgaaatggaa
     2401 tatattgcca tccgcgaaaa tcaggcgcgt gaacaagtcc acaacaacaa aggcggcgaa
     2461 ggcttcggtg caaacctccc ggacttcatc acccccgaat tcgtccgtaa ggaagtcgcc
     2521 gaaggccgcg ctatcatccc cgccaatatc aaccaccctg aatccgaacc gatgatcatt
     2581 ggccgcaact tccttgtgaa gatcaacgcc aatatcggga attcggctgt gaccagctcc
     2641 atcggcgagg aagtcgataa aatggtttgg gccacgcgct ggggcgctga caccatcatg
     2701 gacctttcaa cagggaaaga catccacgat acccgcgaat ggattttgcg caattcgccc
     2761 gtaccgctcg gcactgtgcc aatctaccaa gcgttggaaa aagtcggcgg catcgccgaa
     2821 gacctcacct ttgatatctt tgccgacacg ctggaagaac aggcgcagca gggcgtggat
     2881 tacttcacga ttcacgcggg cgtccgcttg ccttttattc aatacactgc ggatcgtatt
     2941 actggaatcg tttcccgtgg tggctcgatc atggccaaat ggtgcatggc ccaccaccgc
     3001 gaaagcttcc tgtatacaaa attcgatgag atttgcgaaa tcatgaaggc ctacgacgtg
     3061 agcttctccc tcggcgatgg cctgcgcccc ggcgcgatca aggacgcgaa tgaccgcgcg
     3121 caatttgccg agctgaaaac ccttggcgaa ctgacgcgca tagcctggga ccacgacgtg
     3181 caggtcatga tcgaagggcc gggccacgtc cccatgcacc tgattaagga gaacatggaa
     3241 aaagaactgg ccgaatgtca cgaggccccg ttttataccc tcgggccgct caccaccgac
     3301 atcgcgccgg gctacgacca tatcacttct gcgataggag cagcgcaaat cggctggtac
     3361 ggcaccgcga tgctgtgcta cgtaacgcca aaggagcatc tgggtctccc gaatagagac
     3421 gatgtaaaaa ccggcgtcat aacctacaaa atcgccgccc acgcggccga tctcgccaag
     3481 gggcatccgg gtgcgcaaag acgggatgat gctttgagtc tcgcgcgttt cgaattccgc
     3541 tggaacgacc agtttaattt aagcctcgac cccgagacgg ccagaagcat gcacgatgaa
     3601 accctccccg ccgaggggca caaagtcgcc
//
LOCUS       DAOSWR010000176         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_18297_length_3598_cov_5.793395, whole genome shotgun sequence.
ACCESSION   DAOSWR010000176 DAOSWR010000000
VERSION     DAOSWR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18297_length_3598_cov_5.793395"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PK513_10085"
     CDS             complement(<1..1271)
                     /locus_tag="PK513_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO82838.1"
                     /translation="MQQAALKTSPKSQDDFLADGHTPMMAQYLTLKDRHKDCLLFYRM
                     GDFYELFFDDALKASETLDITLTKRGKTAKENGGTDIPMCGVPYHAYEPYLAKLIRAG
                     FKVAICEQTESPEEAKLRAKAEGRPASKTLVNRDVVRVVTQGTLTEDALLQPHENNYL
                     SALTDVGNQYGLAWTDLSTGSFTVQPVLKDQIQTALERIAPREIVIPDTLATELGHDP
                     RHSPQARSLFDSQNAKKKLEDLFDVNTLEGFGAFSRAEIAAAGALIDYIQRTQIGKIP
                     YLEKPRQMTASANMEIDAATRRNLEITRTQSGERKGSLLATIDRTITPPGARLLQTCL
                     SAPLIDIDEINARLNRVEYFTGQSPLRNLLREQLKAMPDMERALSRLSVGRGGPRDLA
                     MIRDGLMQIEIIRAALQNHDGAKEVLSDLLTA"
     gene            complement(1375..2028)
                     /locus_tag="PK513_10090"
     CDS             complement(1375..2028)
                     /locus_tag="PK513_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82839.1"
                     /translation="MKNNSYLILAIVFLFTFTPPSRAEEEAICSSLETCLEMAKEGNA
                     EAQYILAMLYLKEATEIAEENEFEHKTNPRARELYTQHTKWKKEALDQNYAPAQVYEG
                     YQQYTKKFIVPEPSLQEQKDGLQKGAELFLKAAKQGDRRGQFLRAIAVAEDDNTSNDI
                     EGYAWMLLAWKQGLRKYPYRPEIPTKPFLDSYKESLSESDRKEALLLSAEYKTFYSK"
     gene            complement(2032..2484)
                     /gene="dut"
                     /locus_tag="PK513_10095"
     CDS             complement(2032..2484)
                     /gene="dut"
                     /locus_tag="PK513_10095"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOO82840.1"
                     /translation="MSEVRVKLMPLEHAVGLQLPTYATEQSAGMDLSAALEEALELGP
                     GDRALVPTGLSIALPEGYEAQIRPRSGLALKHGVTVLNTPGTIDADYRGEIKVILINH
                     GQHAFTIERGQRIAQMVVEKFETVNWDVVKTLEETDRGEGGFGSTGTE"
     gene            complement(2481..>3598)
                     /gene="coaBC"
                     /locus_tag="PK513_10100"
     CDS             complement(2481..>3598)
                     /gene="coaBC"
                     /locus_tag="PK513_10100"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOO82841.1"
                     /translation="KGGEQFVTPLSVSALCEQEALTGDTYKMDHIHLSRTADAIVIAP
                     ASANIIAQIAHGLAQDLATTALLAADKPALIAPAMNHKMWHNPATQANIATLQSRGHT
                     IIGPEEGDMACGERGLGRMSQPEDILKAVLSNIPTFQHSNILKGRTALVTSGPTLEPL
                     DPVRFIGNRSSGKQGHAIATALAAHGADVTLVTGPVALPDPPGIKTIHIETAQEMLNA
                     CENALNTKEAIDIAVCAAAVSDWSPTKLQNHKIKKRAGKTPPSIALKENPDILKTLST
                     HKNRPMLIIGFAAETENLAKNAADKLKRKKCNWILANLVGQPAQKTFGEDQNHVYLIT
                     SSKTQDWKPMSKDAIATRLVKEIANYFETKAVQDAAE"
BASE COUNT          651 a          754 c         1154 g         1039 t
ORIGIN      
        1 gcggtgagga gatcggagag aacctctttt gcgccatcat gattttgtaa tgcggcgcgg
       61 atgatctcta tttgcatcag tccgtccctg atcatggcga gatcgcgcgg gccgccgcgc
      121 ccgactgaca ggcgtgagag ggcgcgttcc atgtcgggca tggctttgag ctgctcacgg
      181 aggagattgc gcaaagggct ttggcctgta aaatattcga cgcggttcag gcgcgcgttg
      241 atttcatcaa tgtcgatcag tggagcagac aggcaggttt gtagcaggcg cgcgccgggc
      301 ggcgtgatgg tgcggtcgat tgtggccagg agggagcctt tacgctcgcc gctttgggtg
      361 cgggtgattt cgagattacg gcgggtggcg gcgtcaatct ccatattggc cgaagccgtc
      421 atttgccggg gtttttcgag atacggaatt ttaccgatct gggtgcgctg gatatagtcg
      481 atcagcgctc cggcagcggc gatttcggcg cgggagaatg cgccgaagcc ttcgagtgtg
      541 ttgacgtcga agaggtcttc cagttttttc tttgcgtttt ggctgtcgaa taaggaacgg
      601 gcttgcgggc tgtggcgcgg gtcatggccc aattctgtgg cgagtgtgtc agggatgacg
      661 atttcgcgcg gagcaatgcg ttccagcgcg gtctggattt ggtctttgag gacgggttgc
      721 accgtaaatg aaccggtgga caggtctgtc caggccaggc cgtattggtt gccgacatcg
      781 gtgagggctg agagatagtt gttttcgtgg ggctggagga gcgcgtcttc ggtcagggtg
      841 ccctgggtga cgacgcgcac gacatcgcgg ttgacgaggg ttttggaagc ggggcggcct
      901 tcggctttgg cgcgcagctt tgcttcttcg gggctttcag tttgttcgca gatggcgact
      961 ttaaagccgg cgcgaatgag tttggcgagg tagggttcat aggcgtgata agggacgccg
     1021 cacatgggga tgtccgtgcc cccattttct ttggcggttt tgccgcgttt ggtcagggtg
     1081 atgtcgagag tctcggaggc tttgagcgca tcgtcgaaga agagttcata aaaatcgccc
     1141 atacgataga agagcaggca gtctttatgg cggtctttga gcgtgaggta ttgcgccatc
     1201 atcggcgtgt ggccgtcggc gaggaagtca tcttgtgact ttggggatgt ttttaacgca
     1261 gcttgttgca tagggtttat ttaaaccact aagggcgttc aggtcacgaa gaaaaaaatt
     1321 taaaccacag tgaacacagg agaagcacag gggcggtttg tggatggatt ttatctattt
     1381 gctataaaag gttttgtatt ctgctgataa tagtaaggct tcttttctgt cagattccga
     1441 aaggctttct ttataagaat ccaagaatgg tttagtaggt atttccggcc tgtagggata
     1501 ttttcttagc ccttgtttcc aagcaagcag catccatgcg taaccttcaa tatcatttga
     1561 tgtattatcg tcttctgcta cggctattgc tcttaaaaat tgaccgcgtc gatctccttg
     1621 tttagcggct ttcagaaaca attcagctcc tttttgaagg ccatcttttt gttcttgcag
     1681 gctgggttct ggaacaataa attttttggt atattgttga tagccttcgt aaacttgcgc
     1741 aggggcgtag ttttggtcca gagcttcctt tttccatttg gtgtgttgtg tgtataactc
     1801 tctagctctt ggattcgttt tatgctcaaa ttcgttttct tcggctattt cagttgcttc
     1861 ttttaagtat aacattgcca ggatgtactg cgcttctgca tttccttctt ttgccatttc
     1921 caaacaagtt tcaagagatg agcatatggc ttcctcttct gctcgagaag ggggagtaaa
     1981 agtaaaaaga aaaactattg ctaatattag atatgagtta tttttcattt attattccgt
     2041 gccggttgag ccgaagccgc cttcgccgcg gtcggtttct tcgagggttt tgacgacgtc
     2101 ccagtttacg gtttcgaatt tttccacgac catctgggcg attctttgtc cgcgttcaat
     2161 ggtgaaggcg tgttggccgt gattgatcag gatgaccttg atttcaccgc ggtagtcggc
     2221 atcgatggtg ccgggggtgt tgaggacggt gacgccatgt ttgagggcga ggcctgagcg
     2281 cgggcggatt tgagcttcat agccttcggg cagggcgatg gacaggccgg tgggaaccag
     2341 tgcgcggtcg ccggggccga gttcgagggc ttcttcgagg gcggcggaca ggtccatgcc
     2401 ggcggattgt tcggtggcgt aggtggggag ttgaagtcct acggcgtgtt ccaaaggcat
     2461 cagtttaact cttacttcgc tcattctgct gcgtcctgta ctgcttttgt ttcaaagtag
     2521 tttgctattt ctttcactag gcgggtggca atggcgtctt tgctcatggg cttccagtcc
     2581 tgagtttttg aagaggttat aagatagacg tgattctggt cttcgccaaa ggttttttgg
     2641 gcaggctggc caacgagatt ggcgagaatc cagttacatt tctttcgttt gagtttgtcg
     2701 gcggcgtttt tggccaagtt ttcggtttcg gcggcaaagc cgatgatgag catcgggcga
     2761 tttttatgtg tgctcaaggt tttgaggatg tccgggtttt ctttgagcgc gatggagggt
     2821 ggggttttgc cggcgcgttt tttgatttta tggttttgaa gtttggttgg agaccaatcc
     2881 gatacggcgg cggcgcacac agcgatgtca atggcttctt tggtgttgag ggcgttttca
     2941 caggcgttta gcatttcctg ggcagtttca atatggatgg ttttgattcc gggcgggtct
     3001 ggcagggcga ccgggcctgt gacgagggtg acatcggcgc cgtgggcggc cagagcggtt
     3061 gcaatggcat ggccttgttt gcctgatgat cgattgccga tgaagcgtac cgggtcgagg
     3121 ggttcgaggg tcgggccgga ggttacaagg gccgtgcggc cctttagaat gttggaatgt
     3181 tggaatgttg gaatgttgga aagaacggct ttaaggatgt cttcgggctg gctcatgcgt
     3241 cccaagccgc gttcgccgca ggccatgtcg ccttcttcgg ggccgataat agtatgaccg
     3301 cgggattgca gcgtggcgat attggcctgt gttgccgggt tatgccacat tttgtggttc
     3361 atcgccgggg cgatgagggc gggtttgtcg gcggccagta aggcggtggt ggccagatcc
     3421 tgggccagtc cgtgggctat ttgagctatg atattggcgc tggccggggc gatgacgata
     3481 gcgtcggctg tgcgtgagag atggatgtga tccattttgt atgtatctcc ggttagcgct
     3541 tcttgttcgc acagggcgga gacgcttaaa ggcgtgacga attgctcccc gcctttgg
//
LOCUS       DAOSWR010000177         3551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_18672_length_3551_cov_4.529462, whole genome shotgun sequence.
ACCESSION   DAOSWR010000177 DAOSWR010000000
VERSION     DAOSWR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18672_length_3551_cov_4.529462"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PK513_10105"
     CDS             complement(<1..1408)
                     /locus_tag="PK513_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOO82842.1"
                     /translation="MADSQISVAPEDSQDGELYLVDGSGFIFRAYFAMTYSGRGEMTN
                     PDGVPVSAVFGFTQMLMKLLNDYHAPYLAVVFDPKGGSFRNNLYPEYKANRDVPPEDL
                     VPQFPLVREATRAFDIPALEQPGYEADDLIAAYTKRALELGKRVVIVSSDKDLMQLVQ
                     PGVRMLDPMKNKWVGEAEVVEKFGVKPEHVVDVQALAGDSTDNIPGVPGIGVKTAAQL
                     INEYGSLEELLARAEEIKQPKRREKLVNHAQDARVSEQLVRLDQDAPLPLPIEELKIH
                     DPDKPELMAFLQKHAFNSIIKRLGGEFVSVSPAQARTLEEDSRLRGEDTKRAPEIKDN
                     QYTLINDEKTLQEWIDDAYKRGAVCIDTETTALTPAKAKLVGISLTSEVGKAAYIPLA
                     HVAEQADLLGETQGADVPQLPMDKALGMLKPLLEDESVMKVAHNMKYDWQIFAQAGIH
                     VAPCDDTMLISYVLDGSSH"
     gene            complement(1879..2703)
                     /gene="rlmB"
                     /locus_tag="PK513_10110"
     CDS             complement(1879..2703)
                     /gene="rlmB"
                     /locus_tag="PK513_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOO82843.1"
                     /translation="MKKHSKKDKIKHSGKPDTRRHKNPIPQEQRLRTASLYGFHAVRE
                     AWLNENRRVQALYCTQSALDGFQEALETTRKKGLERPAPQILNKQSLEKILPLGAVHQ
                     GLALDCSALDDVGIEDILIRERKNERSLLIILDQVTDPHNVGAILRSACAFGAAGMIV
                     QKKHAPELTGVLAKTACGALEHVPVAFETNLSRCIETLQENGYFVYGLDERGAALATA
                     NIGGKSVLVLGAEGPGLRRLVKEHCDGLLALPMRGPMPSINVSNAAAVALCEFSKN"
     gene            2792..2877
                     /locus_tag="PK513_10115"
     tRNA            2792..2877
                     /locus_tag="PK513_10115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2826..2828,aa:Tyr,seq:gta)
     gene            complement(3170..>3551)
                     /locus_tag="PK513_10120"
     CDS             complement(3170..>3551)
                     /locus_tag="PK513_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82844.1"
                     /translation="SVGVNRVIVDQDELQIDINPLKMRQLLQTHLNLVISEKSALPNL
                     MISVPFTIRRAYRGAIQIQSDHTETDFLDMQPTQLKNLIRGIIWREEHFAGMAMGDIA
                     KSEGLSKAGIRKIIMGSFDVLMAI"
BASE COUNT          882 a          941 c          823 g          905 t
ORIGIN      
        1 tgtgcgaaga accgtccagc acgtaggaga tcagcattgt gtcgtcgcac ggagctacgt
       61 gaattccggc ctgcgcaaaa atctgccagt catatttcat gttatgcgcg accttcatca
      121 cgctttcgtc ctccagtaaa ggtttaagca tccccagcgc tttatccatc ggcagttgag
      181 ggacgtccgc accttgggtc tcgcctaaga ggtcagcttg ctcggccaca tgagcgagtg
      241 gaatataggc cgccttgccc acctcactgg tcagggaaat acccactaat ttggccttgg
      301 ccggggtcag cgccgttgtc tccgtatcga tacacaccgc accgcgctta tatgcatcat
      361 caatccattc ttgcagggtc ttctcatcat taatgagagt atattggtta tcttttattt
      421 caggtgcacg cttagtgtct tctccacgca agcgagaatc ttcttccaag gtccgcgctt
      481 gcgcgggaga aacagaaaca aactcaccac ccaatcgctt gataatcgaa ttaaaagcat
      541 gtttctgcag gaatgccatt aactccggct tgtccgggtc gtggattttt agttcctcga
      601 tcggcagcgg cagcggcgca tcctgatcca gccgcacgag ctgttcggac acgcgcgcat
      661 cctgcgcatg attaaccagt ttttcgcggc gcttgggctg tttaatttct tcagcgcgcg
      721 ccagtaattc ttccagggac ccatattcat tgatcagttg cgccgccgtt ttcacgccaa
      781 tgcccggcac gccgggaata ttatcggtgc tatccccggc cagcgcttgt acgtccacaa
      841 cgtgctccgg cttaacgccg aatttctcaa cgacttccgc ttcgcccacc catttattct
      901 tcatcggatc aagcatgcgc acgccgggct ggacgagctg catcaaatcc ttatcagaag
      961 atacaatcac gacccgtttg cccaactcca gcgcccgttt ggtataagcc gcaatcaaat
     1021 catcggcttc atagcccggt tgttccagcg ccgggatatc aaaggcccgc gtagcctcac
     1081 gcaccagtgg aaattgcggc accagatctt ctggcggcac atcgcggttg gccttatatt
     1141 cgggataaag attattgcga aagctaccgc ccttggggtc aaatactacc gccagatatg
     1201 gcgcgtgata gtcgttcaac aatttcatca gcatctgtgt aaagccgaac accgccgaaa
     1261 ccggaacacc atcggggttg gtcatttcgc cgcgaccaga ataggtcatc gcgaaatacg
     1321 cccggaatat aaaccccgaa ccatcaacaa gataaagctc accatcttga gagtcttcgg
     1381 gagcaacaga aatttgtgaa tcagccatgt cgttaaagtg gcccaagctt ctggcggagt
     1441 ccagagttga tgcttagcca taaacaaaaa aaaccccgca tgcaggggga gagcatgcac
     1501 gggggcggga agtatgggtg aaaccagcct gaagccctac aacagggaaa agagctttaa
     1561 gccttagcgg gtttccgggg gtaacaattg caaaggtaat ttttacgcag gaaaggttgc
     1621 aggaaaggaa ggagcggcct tacgctccga taaaaccgaa aacaagatac gataatgtga
     1681 gattcattat gcgctctgcg cagactcgac tgggcaccga taagctccat gattgcacat
     1741 ccctctactt tttactgccg ttgtccggct cattcttgag aaatcttctt tagcgatttc
     1801 cttgcccttt aattgtacat tgacggctta agagatgtca aaaattttac taaaattgta
     1861 tgtaaagtta gactggatct aatttttgga aaattcgcac agcgccaccg ctgccgcgtt
     1921 tgagacatta atgctcggca tagggccacg cataggcaag gcaagcagac catcgcagtg
     1981 ttctttaact aggcgtcgca gccccggtcc ttctgcgccc agaaccaaaa cggatttccc
     2041 gccaatatta gcagtagcaa gcgccgcgcc gcgctcatct agaccgtaaa caaaatagcc
     2101 attttcctgc agagtttcga tacagcggct taagttcgtt tcaaaagcca caggcacatg
     2161 ctccaatgcg ccgcaggccg tcttggccaa aacgccggtt agctcaggcg catgtttctt
     2221 ttgtacaatc atccctgccg ccccaaatgc acaggccgaa cgcaaaatcg cgcccacatt
     2281 atgcgggtcg gtaacctgat caagaataat cagcaagctg cgctcatttt tgcgctcacg
     2341 aataagaatg tcttcaattc cgacatcgtc cagcgcgctg cagtccagcg ccaaaccttg
     2401 atgcaccgcg ccaagtggca aaatcttctc taaagattgt ttattaagaa tttgcggcgc
     2461 cggacgctct aagccttttt tccttgtagt ctccagagct tcctgaaaac catccagcgc
     2521 actttgtgta caatacagcg cctgaacgcg acgattttca ttcagccacg cctcacgtac
     2581 cgcatgaaat ccataaaggc tagcagtacg taaacgctgc tcttgcggta tagggttttt
     2641 gtgacggcga gtatccggtt tgccgctatg cttgattttg tcttttttcg agtgtttttt
     2701 catcgcccag cagattacag gcttgacatc aggggtgaaa gtcttttttc tgctgtttaa
     2761 atctcacacg ggattttctt attatatgtg tggaggggtg gtcgagtggt taatggcacc
     2821 tgactgtaac tcaggcctct tcggagtacg ctggttcgaa tccagccccc tccaccattt
     2881 agtccggctt tctggacatt cggagaattt ctccccgcaa gccccgctga cagcggggtt
     2941 tttgttttgg cgcatcgccg agacacacag agaatttcat ttttgggctc agaactacca
     3001 tttttctcta cagcgcattt ttgagtgtcc cgagatcctg aattccctgt ttatcaggga
     3061 attaacaggg aatttttcta aattttgaat gctgaagatg aaacaaagcc agagaatatc
     3121 tgtgcttcag aacaattcac aatcaaaata aacagggaaa tatcgagaat tatattgcca
     3181 taagcacatc aaagctgccc atgatgatct tacggatgcc agctttggac aaaccttcac
     3241 tcttggcaat atcgcccatc gccattccgg cgaaatgttc ctcacgccag ataatgccac
     3301 ggatcaggtt ctttagctgt gtcggctgca tatccaagaa atcagtttcc gtatgatccg
     3361 attgtatctg aatggcacct ctgtaagctc gtctgatggt aaaaggtacg gagatcataa
     3421 ggttcggcaa tgcagatttt tctgaaatca caagattgag atgcgtctgc aaaagctgtc
     3481 gcatcttcag aggattgata tcgatttgaa gctcatcctg atccacgata actctgttta
     3541 cgcccacgct c
//
LOCUS       DAOSWR010000178         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_19224_length_3480_cov_5.362920, whole genome shotgun sequence.
ACCESSION   DAOSWR010000178 DAOSWR010000000
VERSION     DAOSWR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19224_length_3480_cov_5.362920"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="narZ"
                     /locus_tag="PK513_10125"
     CDS             complement(<1..471)
                     /gene="narZ"
                     /locus_tag="PK513_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005972290.1"
                     /note="with NarYV catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli;
                     expression of nitrate reductase Z is not dependent on
                     nitrate levels; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOO82845.1"
                     /translation="MSYFIDRITYFSQNREKFSGGHGVTTEENRAWEQAYRDRWAHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLITWETQQTDYPRTRPDLPNHEPRGCARGASYSWY
                     IYSANRLKYPMVRKRLLKLYRKAKETTKDPVDAWAAIQADEELRREYQQVRGRGG"
     gene            complement(492..2102)
                     /locus_tag="PK513_10130"
     CDS             complement(492..2102)
                     /locus_tag="PK513_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO82846.1"
                     /translation="MAKDIQTWDPENEEQWKSGGASIAWRNLWISIPCLLCGFAVWLY
                     WGVITVQMLNLGFPFEQSELFTLSAIAGLTGATLRIPSTFFIRLAGGRNTIFFTTALL
                     ILPALGTGFALQDKDTPLWMFQLLALLSGFGGGNFASSMSNISFFFPKKVQGLSLGLN
                     AGLGNAGVTTMQILIPLFMTFAAFGGEPMILENTSGTLIGKIEAGSETWIHNAGFVWV
                     AILVPLVIAAWFGMNNIRAEHVSPNIAPAPVAFFQISVMLLIGFITATFGLWLMLPEA
                     ANGSGFGVSKWLVLPIVIALTVFLLKMIPGETGQNLKRQYKIFNHSHTWIMTIIYTMT
                     FGSFIGYAASFPLAIKVIFGFQHVVNEAGIMTHDIANPNGPSALMYAWMGPFIGAFIR
                     PLGGWISDKMGGALVTQICSVIMVASALGVAYYMKAAYASATPEQYFVPFFVLFLVLF
                     AATGIGNGSTFRTIAMVFDREQAGPVLGWTSAVAAYGAFIIPKVLGEQIKLTTPEIAL
                     YGFAAFYAFCCLLNWWAYLRPGAEHKNP"
     gene            2282..>3480
                     /locus_tag="PK513_10135"
     CDS             2282..>3480
                     /locus_tag="PK513_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO82847.1"
                     /translation="MELENKATALWANFFKVNMVQIRTFHMTWFAFFLCFFAWFGIAP
                     LMKVVREELALTKEQVGWTIIASVAVTIFARLLIGWLCDRIGPRLTYTWLLVLGSLPV
                     MGIGLAHNFETFILFRLMIGVIGASFVITQYHTSVMFAPNVVGQANATSAGWGNLGGG
                     VTQFVMPLIFSIFVLGFGFSDEFGWRASMFVAGIVCFITGIAYYFFTQDAPDGNYKDL
                     RAAGKMPEKKQVTGGYFRAMKDYRVWLLFLIYAACFGIELTVNNVAALYFADYFDLSL
                     VAAGMTAAAFGLMNIFARTLGGVFGDNFASLWGLKGRAMWLFIVLFCEGLALMLFSQM
                     NVLFLAIPTLILFSLFTQMSEGATYSVVPFINRKALGAVAGIVGAGGNAGAVAAGFLF
                     KGSMDWAT"
BASE COUNT          858 a          884 c          871 g          867 t
ORIGIN      
        1 tccgccgcgt ccgcgtacct gctggtattc acggcgcagt tcttcatcag cctgtatagc
       61 agcccaggca tcgacaggat ctttcgttgt ttcctttgcc ttgcgataca gcttgagcag
      121 ccgcttgcgc accatcggat atttcaagcg gttggcagaa taaatatacc agctgtaact
      181 cgccccacgc gcgcaaccgc gcggctcgtg gttgggcagg tcaggccgcg tccggggata
      241 atctgtctgc tgcgtttccc acgtaataag cccgtttttg acataaattt tccatgagca
      301 ggagcctgtg cagttcacac catgcgtgga ccggacgatt ttgtcatgtg cccaacggtc
      361 acggtatgcc tgttcccaag ccctgttttc ttcagttgtt acaccgtgcc cacctgagaa
      421 cttttcacga ttctgggaaa aatacgtgat tcggtcaata aaatagctca tcgtggtttc
      481 tctcgtttct cttaaggatt tttatgctct gcgccaggac gcaggtaggc ccaccagttc
      541 agaaggcagc aaaacgcata gaaggctgca aagccgtaaa gcgcaatttc cggtgttgtc
      601 agcttaatct gttcgcctaa aactttgggg ataataaagg cgccgtaagc ggcaacggca
      661 gaggtccatc caagtacagg cccggcctgt tcacggtcga agaccatggc aatcgtgcgg
      721 aacgtcgagc cgttgccaat ccctgtagcg gcaaacagca caaggaacag aacaaagaac
      781 ggcacaaaat attgctcagg cgttgccgac gcataggctg ctttcatata gtaagccaca
      841 cccaaagcac tagccaccat aattacggag caaatttgcg tgaccagcgc cccgcccatt
      901 ttgtcggaaa tccagccgcc cagggggcgg ataaaagcac cgataaacgg gcccatccac
      961 gcatacatca gggcactggg accgttgggg ttggcaatgt catgcgtcat gataccggcc
     1021 tcgtttacga catgctgaaa accgaagatc actttgatgg ctagcgggaa agaagccgca
     1081 tagccgatga aagatccaaa tgtcatggta taaatgattg tcatgatcca tgtatgcgaa
     1141 tggttgaaaa tcttatactg acgctttaga ttttgacccg tttctccggg aatcattttg
     1201 agcagaaaca ccgttaacgc gataacaatc ggcagcacaa gccatttgct gaccccaaaa
     1261 cccgaaccat tcgccgcttc gggcaacata agccacaagc caaacgttgc cgtaataaag
     1321 ccgatcagca gcatcacgga aatttgaaag aatgcaacag gagcgggcgc gatatttggg
     1381 gaaacatgct cggcacggat attgttcata ccgaaccatg cagcaatgac aagcggcacc
     1441 aaaatagcaa cccagacaaa accggcattg tgaatccatg tctcggatcc ggcctcgatc
     1501 ttgccaatca acgttccgct cgtgttttca agaatcatcg gctccccgcc gaaagcggca
     1561 aatgtcataa agagcggaat caatatctgc attgttgtca caccggcatt tcccagacca
     1621 gcattcaaac ccagagaaag cccctgcacc tttttcggga agaaaaagct gatattggac
     1681 atggaagacg caaaattccc gccgccaaaa ccggaaagga gagccagaag ctgaaacatc
     1741 cacagcggtg tatctttatc ttgcagcgca aaacctgtgc ctagggcagg gaggattaaa
     1801 agcgcagtcg taaagaagat tgtgtttcgt ccaccggcca gacgaataaa aaacgtggaa
     1861 ggaatgcgca gcgtcgcgcc ggttaatccc gcaatggccg acaaggtaaa cagctcggac
     1921 tgctcgaacg ggaagcccag attgagcatt tggacggtga taacacccca gtagagccag
     1981 acagcaaagc cacacagcag gcagggaatg gaaatccaca aatttcgcca ggcaatcgaa
     2041 gctccgccgc ttttccattg ctcttcgttc tccggatccc atgtttgaat atctttggcc
     2101 attcttagct tccttccaaa tttataaatg ccgactccgc atttttgcag ttttagaaaa
     2161 gatggacttg attcagatca aggaggttgt ctgatttcat atctagcttc tgaaaaagag
     2221 gcaaaagcca cctgcaaatc tacctccatc ccgccctcaa tccagaatcg gagtaaacac
     2281 catggaactc gaaaataaag ctacggccct gtgggcaaac ttcttcaagg tcaatatggt
     2341 ccagataaga acatttcaca tgacgtggtt tgccttcttt ctgtgtttct ttgcctggtt
     2401 cggcattgcc cctcttatga aggttgtccg tgaagaactt gcactgacca aggaacaggt
     2461 tggctggacc atcattgctt ccgtggcagt gactatcttt gcccggcttc ttataggatg
     2521 gctgtgcgac cggatcggtc cgcgcctgac ctatacatgg cttcttgttc taggctcttt
     2581 acccgttatg gggattggtt tggctcataa tttcgagaca ttcatcctgt tccgcttgat
     2641 gattggtgtg attggcgcgt catttgttat tacgcagtat catacttccg tcatgttcgc
     2701 cccgaatgtt gtggggcagg caaatgcgac aagtgccgga tgggggaatc ttggcggcgg
     2761 cgtaacgcag tttgtcatgc ctctgatttt cagcatattc gttttgggct tcggtttttc
     2821 cgatgaattc ggctggcgcg cttccatgtt tgtcgccggg atcgtctgtt tcatcacagg
     2881 gattgcatac tatttcttta cccaggatgc tccagacggc aattacaagg atcttcgtgc
     2941 ggccggaaaa atgccggaaa agaaacaagt caccgggggg tatttcagag cgatgaaaga
     3001 ctaccgggtc tggcttctgt tcctgattta cgcagcctgt ttcggcattg aactcaccgt
     3061 caacaacgtt gccgcccttt atttcgcaga ctactttgat ctgagccttg tcgcagcggg
     3121 tatgacagcg gcggcgttcg ggctgatgaa tatttttgcc agaacgttag gcggagtttt
     3181 cggcgacaac ttcgcatcac tctggggttt gaaaggccgt gcgatgtggc tttttatcgt
     3241 cttattctgc gaaggtctgg ctttaatgtt attttctcag atgaacgtcc tgtttctggc
     3301 tattccaacg ctgatccttt tcagcttgtt tacgcaaatg tcggaaggag caacttattc
     3361 tgttgtgccg ttcatcaaca gaaaagctct cggtgctgtg gctggtatcg tcggagctgg
     3421 agggaatgcc ggcgccgtgg ccgctgggtt tttgttcaaa ggttctatgg actgggcaac
//
LOCUS       DAOSWR010000179         3409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_19864_length_3409_cov_5.515206, whole genome shotgun sequence.
ACCESSION   DAOSWR010000179 DAOSWR010000000
VERSION     DAOSWR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19864_length_3409_cov_5.515206"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK513_10140"
     CDS             complement(<1..656)
                     /locus_tag="PK513_10140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOO82848.1"
                     /translation="MAVTSPILNVMIKAAEKAARSLIRDFGEVEQLQVSQKGPGDFVS
                     AADKRAEELIYEELKKGQPTYGFLMEESGERAGSNPDFRWIVDPLDGTTNFLHGIPHW
                     CISIALEAKGELTHGLIYDPVKDEMFTAEKNGGAFIHRNKRLRVSGRQDMMRATIATG
                     APRRAVVDQERFLREYAAALNVSPGLRRFGAAALDLAYVAAGRYEAFWERKLQPWDVA
                     A"
     gene            complement(656..1222)
                     /gene="efp"
                     /locus_tag="PK513_10145"
     CDS             complement(656..1222)
                     /gene="efp"
                     /locus_tag="PK513_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO82849.1"
                     /translation="MKVNAITLRSGNVIDYNGKLWAVVKNDIQQPGKGASVAQVELRD
                     IRSGSKDNVRFRTQETVERVRLEQDNYQFLFADGEDYTFMHQETFEQIVINKELIGQS
                     AAYLVEGMIVEIETFEGEPLSVKLPDTVTVEIVEAEPVVKGQTATTSYKPAILANGEK
                     VMVPPHIESGTRIVVKSEDGTYVERAKD"
     gene            complement(1219..1686)
                     /locus_tag="PK513_10150"
     CDS             complement(1219..1686)
                     /locus_tag="PK513_10150"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOO82850.1"
                     /translation="MRPLSEATAKIAGKSFERKYIALGRIVNNWRDIVGEKLADKGQP
                     VKIHYRKKKGQKKPDATLEIAANSADATLLHYQKDLILERINQIFGERWITAIKFVNV
                     ASNTQTKTFKKPKAPLTPAEKKTLSDVLLNIEDEDMKARLEHLGQSIIMDEKA"
     gene            1728..2825
                     /gene="mutY"
                     /locus_tag="PK513_10155"
     CDS             1728..2825
                     /gene="mutY"
                     /locus_tag="PK513_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOO82851.1"
                     /translation="MTVTIANSKLSVNDIEKVRGQLLDWYDAHARDIPWRAKAGQAPD
                     PYHVWLSEIMCQQTTVQAVKAYYLKFLELWPSVQDLAAADQDDVMAAWAGLGYYARAR
                     NLHKCAKVVVNELGGVFPSRQEQLKKLPGIGDYTSAAIAAIAFNQPTTVMDGNVERII
                     ARFFAVREPLPKAKPQLKALVHQFYDDFTVRPGDLAQSFMDLGAGICIPKIPRCGLCP
                     LQGACQAHRQGIAAELPAKIKAKTRPQKYGNIYWISNDQGQVLFHQRQEKGLLGGMLG
                     LPTSNWKADNTTLTHPEYINTPTPLHIQVQHTFTHFDLKLDLFKATLKAKLPIKDIKL
                     CWLCPMSAGNKLPSVFKKAHKILVSPNKQKP"
     gene            2898..>3409
                     /locus_tag="PK513_10160"
     CDS             2898..>3409
                     /locus_tag="PK513_10160"
                     /inference="COORDINATES: protein motif:HMM:NF027470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HOO82852.1"
                     /translation="MFKKIGVWIGVIIVGVMASVSYAQESPEQGPVIYESPTIKNLSQ
                     LYWSLSKFKPDENGKIDDIYIDNFLFINECSLYQEFSDNEFEWVNIRESARNFLEENK
                     SGFPLRFEYTQPLRLAEYDLESQTFDVWEPYKINGIRRFEILAEDLFEDICNQPAKYA
                     IEGYPKGLFA"
BASE COUNT          922 a          867 c          741 g          879 t
ORIGIN      
        1 gctgccacat cccagggctg gagtttgcgt tcccaaaagg cttcataacg tccagcggca
       61 acataagcca gatccagcgc cgcagcgcca aaacgccgca aaccggggga aacatttagc
      121 gcagcggcat attcccttaa aaaacgctcc tgatcaacaa cagcgcggcg cggcgctcca
      181 gttgcgatag tagcacgcat catgtcctga cgcccggaaa cccgcaggcg tttattacgg
      241 tgaataaacg ccccgccatt tttttcggct gtgaacatct catcttttac cggatcataa
      301 atcaaaccgt gcgtcaactc gcctttagct tctaatgcaa tcgaaataca ccagtgcgga
      361 atgccgtgca ggaagttcgt tgttccgtcc agaggatcga caatccatcg aaaatcagga
      421 ttagagccag cgcgttcacc gctttcttcc atcaggaagc cataagtcgg ctggcctttt
      481 ttcagctctt cataaatcag ttcttcggcg cgtttatcag cagcgctgac aaaatcaccg
      541 gggcctttct gcgaaacctg aagctgctca acttcgccaa aatcacggat caatgatcgc
      601 gcggcttttt ccgccgcctt gatcatcaca ttgagaatag gggacgtaac agccatcaat
      661 ccttcgcccg ctcgacatag gtgccgtctt cactcttcac cacaatccgc gtacccgatt
      721 caatatgcgg cggcaccatc actttttcgc cattggccaa aatagccggt ttataagatg
      781 tcgtagcggt ttgacctttc acaacaggct cggcctcgac aatctcaacc gtaactgtgt
      841 cgggcaattt aacgctcagc ggctcacctt caaaagtttc gatctcaaca atcatcccct
      901 caaccagata cgccgcactc tgtccgatca gttctttgtt aataacaatc tgttcaaaag
      961 tctcttggtg cataaaggta taatcctcac catcggcaaa caggaattga taattgtcct
     1021 gctccaggcg tacgcgttca acagtttctt gtgtccgaaa acgcacgtta tccttgctgc
     1081 cgctgcgaat atcgcgcagt tcaacttgcg ccaccgacgc gcccttgccg ggctgctgaa
     1141 tgtcattttt tactacggcc caaagcttgc cgttgtaatc gatcacgttc ccggatcgta
     1201 gtgtaatagc gttaaccttc atgctttttc atccattatg attgattgac ccagatgctc
     1261 gaggcgggct ttcatatcct catcctcaat attcaacaac acatcggata aagtcttttt
     1321 ttcggcgggc gtcaaggggg cttttggttt tttgaacgtt ttcgtctgcg tattagacgc
     1381 aacattgaca aatttaatcg ccgtaatcca gcgctcgcca aaaatctgat tgatccgttc
     1441 taaaataagg tctttttgat aatgaagcaa tgtcgcatcc gcgctattgg ccgcaatttc
     1501 cagcgtggca tcaggttttt tctgtccctt cttcttgcga taatgaattt taaccggctg
     1561 ccccttatcg gcaagcttct ctccgacaat atcgcgccag ttatttacaa tcctccccag
     1621 cgcaatatat ttacgctcaa agctctttcc cgcgatttta gccgtagcct ctgacagggg
     1681 gcgcatgcta ttcaaactct ctactttatt gtgaagccgg aacgaccatg acagtgacca
     1741 tagcaaacag caaactttcc gtaaatgata ttgaaaaagt tcggggacaa ttgctggatt
     1801 ggtatgatgc tcatgctcgc gatattccat ggcgagctaa agcagggcag gcacccgatc
     1861 cctatcatgt ctggttatcc gaaattatgt gccagcaaac caccgtacag gcggttaagg
     1921 cgtattatct gaaatttctt gaattatggc caagcgtaca agatcttgcc gccgccgatc
     1981 aggatgatgt aatggccgca tgggcaggtc ttggctatta cgctcgcgca cgtaacctgc
     2041 ataaatgcgc caaagttgta gtgaatgaat tgggtggcgt atttccatct aggcaggagc
     2101 aactcaaaaa actcccaggc ataggggact acaccagcgc cgcgatcgct gccattgcct
     2161 tcaaccagcc caccactgtc atggacggta atgttgaacg catcatcgct cgtttttttg
     2221 ctgtacgcga accattacca aaagcaaaac cacagttaaa agcgcttgta catcaattct
     2281 atgacgattt tactgtacgt cccggcgatt tggcgcaaag ctttatggat ttgggggcgg
     2341 gtatttgcat ccccaaaatc ccgcgctgcg gcctctgccc tctacaaggg gcttgtcagg
     2401 cgcatcggca aggcatcgcc gccgagctac ctgcaaaaat caaagccaaa acccgcccgc
     2461 aaaaatacgg caatatctac tggatcagca acgatcaagg gcaggttctg tttcatcagc
     2521 gccaagaaaa agggctgctt ggaggtatgc tgggcttgcc tacctctaat tggaaggcgg
     2581 acaataccac cctcacacac ccagagtata tcaatacacc aactccctta catattcagg
     2641 ttcagcacac attcacgcat ttcgatctga aactcgattt attcaaagca acgctaaaag
     2701 cgaaactgcc catcaaagat ataaaattgt gctggttatg cccgatgagt gcaggaaaca
     2761 aactgcccag cgtatttaaa aaggcgcaca agatactggt gtcgcccaat aaacaaaaac
     2821 cttgaagttg cgagttaacg ctaaccccag tataattaaa caatgaggat gcgcataaac
     2881 agaaagtgaa tggggtaatg tttaaaaaga taggggtgtg gattggtgtt attatagtag
     2941 gtgttatggc gtctgtttcc tacgctcaag aaagtcctga acaagggccg gtcatatatg
     3001 aatcccccac aattaaaaac ctttctcagc tctactggtc attatcaaaa ttcaagccgg
     3061 acgagaatgg caaaattgat gacatataca ttgataattt cttatttatt aatgagtgta
     3121 gcctgtatca agaattctca gataatgaat ttgaatgggt aaacataaga gaatctgcca
     3181 gaaattttct tgaagagaat aaatctggat tccctctgag atttgaatac acccagccgc
     3241 tgcggctagc agaatacgat cttgagagtc agacattcga tgtatgggaa ccctataaaa
     3301 ttaatggaat tcggcgtttt gaaattttag cagaagacct gtttgaggat atttgtaatc
     3361 agccggctaa atatgcaatt gagggttatc caaagggcct gtttgcaga
//
LOCUS       DAOSWR010000180         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_19923_length_3403_cov_4.735663, whole genome shotgun sequence.
ACCESSION   DAOSWR010000180 DAOSWR010000000
VERSION     DAOSWR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19923_length_3403_cov_4.735663"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PK513_10165"
     CDS             <1..750
                     /locus_tag="PK513_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOO82853.1"
                     /translation="GGGVHMGYPLSERWRQGLNYRMERNEITDVQSDASRFIRGQEGT
                     RDTSALSQRLTYDSRDSTVFPTEGLLYWLNAEVAGLGGDAKYVSGKTGVSYYYPVFDQ
                     VVLNAMAETGIIEGYSDTDVQINERYYLGGSTLRGFESAGVGPRDRTTGDALGGQVFY
                     RGTLESSFPIGLPSELGISGHAFGDVGTLTELDDNGPEIQDEASLRASLGLGLSWRSP
                     FGPVRADFAVPVSKEDFDQEEHFRFNFGTRF"
     gene            805..1344
                     /locus_tag="PK513_10170"
     CDS             805..1344
                     /locus_tag="PK513_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOO82854.1"
                     /translation="MRLFCLFLSFFSVFIWPVCSQAAGSIGVVDVAYILSKSQAAQNI
                     QAQRQSVREAFLADISKTEQALRAEEKKILDQRGKISQEDYLKLRQVYEANLLELRKD
                     AQQKKRALEEASNVAMNVLREELYVVVQEIANERGFELVISNKNVIAGEKSLDITKET
                     LEIINKNLKEVPLKIEEVE"
     gene            1344..2366
                     /gene="lpxD"
                     /locus_tag="PK513_10175"
     CDS             1344..2366
                     /gene="lpxD"
                     /locus_tag="PK513_10175"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOO82855.1"
                     /translation="MADSRFFVKSKDFSLRELVELTGSELGAGAAPERVLSDVAPLDI
                     AGPDDLSFLDNVKYKDQFLTTRAGACFVHPSVTELAPRNVCLLLNVHPYKAYALAAQA
                     FYPDAKPNGEISEQAYIDPSARIGGECTIGPGVVVGAGVEIGEASWIEANSVIGDNVR
                     IGAYCRIGSNATLSHCHIGDYTRLYPGVRVGQDGFGFAIDPKGHVKVPQLGRVIIEDH
                     VEIGANTTIDRGAGPDTIIGQGTWIDNLVQIGHNVRIGRGCVIVAQVGISGSTVIEDF
                     VVIGGQAGVTGHLHIGSGARIAAQSGVMRNVPAGEEYMGSPAMPIRDNMKQVALLKRL
                     IKKNKG"
     gene            2397..2846
                     /gene="fabZ"
                     /locus_tag="PK513_10180"
     CDS             2397..2846
                     /gene="fabZ"
                     /locus_tag="PK513_10180"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOO82856.1"
                     /translation="MSTIDINRIMEMIPHRYPILLVDRVLELMPGESATSLKNVTMNE
                     PHFMGHFPGYPVMPGVLIVEAMAQTAAIVVVEALGAQAEGKVVYFMTIDNARFRRPVT
                     PGDSLHIHVEKLQSRAHVWKFKGTASVEGKICAEATFSAMITDKESA"
     gene            2937..>3403
                     /gene="lpxA"
                     /locus_tag="PK513_10185"
     CDS             2937..>3403
                     /gene="lpxA"
                     /locus_tag="PK513_10185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOO82857.1"
                     /translation="MSAPQIHPSAIIDEKAVLADGVKIGPYCVIGPDVSLGADVELKS
                     HVVIDGCTEIGAGCTIYPFVSLGAAPQDLKYAGEKSRLIIGQNNTIREHTTMNPGTAD
                     GGMVTRVGNNNLFMMSTHVAHDCQVGNHVIMANNATLGGHVQVGDFVIIGGLAA"
BASE COUNT          798 a          603 c         1022 g          980 t
ORIGIN      
        1 ggtggcgggg tgcatatggg ctatccgctt tctgagcgtt ggcgtcaggg tttgaactat
       61 cgtatggagc gtaacgagat taccgatgtg caaagtgatg cctctcgctt tattcgtggt
      121 caggaaggga cgcgggatac gtctgcgctg tcgcagcgtt tgacctatga tagccgcgat
      181 agcacggtct ttccgacgga gggattgttg tactggttga atgcggaagt ggccggtttg
      241 ggcggtgatg ccaagtatgt ttcagggaaa acgggcgtgt cttattacta tccggtgttt
      301 gatcaggtgg ttttgaatgc tatggctgaa accgggatta ttgaagggta tagcgatacg
      361 gatgttcaga tcaatgaacg ctattatctg ggtggcagta cgcttcgcgg ttttgaaagc
      421 gcaggcgtcg ggccgcgtga ccggacgact ggtgatgctc ttggcggtca ggtcttttat
      481 cgtggaacgc tggaaagctc tttcccgatt ggtttgccga gtgagcttgg tatttcaggg
      541 catgcatttg gcgatgtcgg gacattgaca gagttggatg ataacggtcc ggaaattcaa
      601 gatgaggcca gtttacgggc aagcttggga ttggggttgt cgtggcgctc tccattcggg
      661 cctgtgcgcg ctgactttgc tgtgccggtt tccaaggaag actttgacca agaagagcat
      721 ttcagattta actttggcac acgcttttaa atctgatact catgcaaggc gttataattt
      781 tgttatagct atgaagtggg tatcatgcgt ttattttgtc tgttcttaag tttttttagt
      841 gtttttatat ggcctgtgtg ctcgcaggcc gcagggtcta tcggtgttgt tgatgttgct
      901 tatattttga gtaaatcaca agcggctcag aatattcagg cgcagcggca gagtgtgcgg
      961 gaggcgtttt tagctgatat ttccaagact gagcaggctt tgcgggcgga agaaaaaaaa
     1021 attcttgatc agcgcggtaa gatttcacaa gaagattatt tgaaacttcg tcaggtttat
     1081 gaggctaatc tccttgaact gcgtaaagac gctcaacaaa agaagagagc cttggaagag
     1141 gcttccaatg tggctatgaa tgtgttgcgc gaagagcttt acgttgttgt gcaagagatt
     1201 gccaatgagc ggggatttga gttggttatt tctaataaaa atgtcattgc gggtgagaag
     1261 tcgcttgata ttaccaaaga gacgcttgag ataattaata aaaatttgaa ggaagtgcct
     1321 ttgaaaattg aggaggtcga atagtggcgg actctcggtt ttttgtaaaa tccaaggatt
     1381 ttagcttgcg ggagcttgtg gaactgacgg gttctgaact cggggctgga gcggcgcctg
     1441 aacgcgtttt gagcgatgta gcgccgctgg atatagccgg gccggatgat ttgagttttc
     1501 ttgataatgt aaaatataaa gaccagtttt tgacaaccag ggccggggca tgttttgtgc
     1561 atccatctgt aacggagcta gcccctcgaa atgtctgttt gttgcttaat gtgcaccctt
     1621 ataaggctta tgcgttggcg gcgcaggcat tttaccctga tgccaagccg aatggtgaga
     1681 tatcagagca ggcgtatatt gatccttctg cgcgtattgg tggtgaatgt acaatcgggc
     1741 cgggcgtagt ggttggcgcg ggtgttgaaa tcggagaggc aagctggatc gaggcgaata
     1801 gcgttattgg tgacaatgta cgtattggcg catattgtcg cattggttcg aatgcgacgc
     1861 tttcgcattg tcatattggt gattatacgc ggctgtatcc gggcgtacgt gtggggcaag
     1921 atggttttgg gtttgctatt gatcctaaag gacatgtcaa agttcctcaa ttgggccggg
     1981 tgattattga ggatcatgtc gaaattggcg cgaatacgac gattgatcgt ggggcagggc
     2041 ctgatactat aattgggcaa ggtacatgga tagataatct ggtgcagatc ggtcataacg
     2101 ttagaattgg acggggatgc gtgattgttg cgcaggtagg gatttctggt tcaacggtaa
     2161 ttgaagactt tgtcgtgatc ggtggccagg ctggtgtgac cgggcatttg cacattggca
     2221 gtggtgcgcg tatcgctgcg caatctggtg ttatgcgcaa cgtgccagct ggagaagaat
     2281 atatggggtc tccggcgatg cctattcgcg ataatatgaa acaggttgct ttactgaaac
     2341 gtttgattaa aaagaataaa ggataatttt ggtagactta taggattaat cagatcatga
     2401 gcacaattga tataaatcgt attatggaga tgattccgca ccgttatcct attttactgg
     2461 tggatagggt tcttgaattg atgcctggtg agagtgcaac aagccttaaa aatgtgacga
     2521 tgaatgagcc gcattttatg gggcatttcc cggggtatcc tgttatgccg ggggttttga
     2581 ttgttgaggc gatggcgcag acagcggcga tcgttgttgt ggaagctttg ggcgcgcagg
     2641 cggaaggcaa agttgtttat tttatgacga ttgataatgc gcgtttccgc cggccggtaa
     2701 cgcctggtga ttccttacat atacatgttg aaaagttgca atcgcgcgct catgtctgga
     2761 aatttaaggg cacagcgagc gttgaaggca aaatctgtgc ggaggcaaca tttagcgcga
     2821 tgatcaccga taaagaatcg gcctagtttt tcttctttaa tttgtaacgc agtgcaacaa
     2881 tccgtgattt gtgcaaacgt ctttctgagc ctagtatttt gaaaaaaggt tagagtatga
     2941 gtgcgcccca aattcaccct agtgccatta ttgatgaaaa ggctgtgttg gccgatggtg
     3001 tcaagatcgg tccatattgc gtgattggtc cggatgtttc tttgggcgca gatgtggagt
     3061 tgaaatcgca tgtcgtgatt gatggatgca cagaaattgg cgcaggttgt acgatttatc
     3121 cgtttgtgtc tttgggggcc gcaccacagg atttgaaata tgccggtgag aagtcccggc
     3181 tcattattgg tcaaaacaat acaatccgcg aacatacgac aatgaatccc ggcactgcgg
     3241 atggggggat ggtgacgcgt gttggtaata ataacctatt catgatgagc acacatgtgg
     3301 cgcatgattg ccaggtgggt aatcatgtga ttatggcgaa caatgcaaca ttgggtgggc
     3361 acgtacaggt tggtgatttt gtaattatcg gcggcttggc ggc
//
LOCUS       DAOSWR010000181         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_20216_length_3373_cov_4.219711, whole genome shotgun sequence.
ACCESSION   DAOSWR010000181 DAOSWR010000000
VERSION     DAOSWR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20216_length_3373_cov_4.219711"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1287)
                     /gene="odhB"
                     /locus_tag="PK513_10190"
     CDS             complement(13..1287)
                     /gene="odhB"
                     /locus_tag="PK513_10190"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOO82858.1"
                     /translation="MTQIVVPTLGESVSEATVAQWLKKEGEAVKADEPIVELETDKVT
                     LEVNAPADGVIVKITVGEGETVEVGALLGEMGEGAAANDTPAAPAKDEAPKEEAPKTV
                     EAAPAPAKEEVASAPEASADAKTSPSVRKMLSDNNLEAAQVPASGERLSKEDVQKFIA
                     DNKGAKAASLSPPPSAPAPASDAHPPRTETGPREERVRMTKLRQVIATRLKEAQNNAA
                     MLTTFNEVDMGPVMELRKAYQDQFVKKHGIKLGFMSFFVKAAINALKEFPAVNAEIDG
                     TDIVYKNYYDIGVAVSTPQGLVVPVIRDADQMSMAEIEAKIVDLGVRARDGKLSIDEM
                     TGGTFTITNGGIFGSMLSTPILNAPQSAILGMHNIVQRPMVMPNGAIEARPIMYLAHS
                     YDHRIIDGREAVSFLVRIKQAIEDPARLVLDV"
     gene            complement(1375..2271)
                     /gene="thiL"
                     /locus_tag="PK513_10195"
     CDS             complement(1375..2271)
                     /gene="thiL"
                     /locus_tag="PK513_10195"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOO82859.1"
                     /translation="MAEFDLIEKYFAPLSMDGLKNDTAVLDIPAGHQLVVSSDTSNRG
                     DHFVEEMSAFDAGRKCLRSNVSDLVSSGADPLAYQLNLALPKDLDEDWLAGFADGLAA
                     DQALYNIALSGGDTTGMRAGGLSVSITAMGLVPTGRAVLRSGARAGDVVVITGILGDA
                     YLSRLHMPSPPVVLAPLVRKYAKAAVDISDGILADLGHICSVSGLGARLEFDKIPLSD
                     KGRAALRDGKASSEDLLTWGEDYELAMAVSPSDVEAFQKQAGALGVALSVVGVFEAVA
                     GVKVFDQDGHEMSFSKTGWQHF"
     gene            complement(2404..3030)
                     /gene="thiE"
                     /locus_tag="PK513_10200"
     CDS             complement(2404..3030)
                     /gene="thiE"
                     /locus_tag="PK513_10200"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOO82860.1"
                     /translation="MVKPFNPSIYFVADPLLCAGRDIVDVVAAALRGGVTMVQYRNKF
                     DPMPDILAEAAMLQEWMTPGVAFIINDHIDVAQAVGAAGVHLGQGDSSPEEARAKLGA
                     AAIIGQTAFTPEHMAAIDPGVVDYVGTGPFYETQTDKGKPVLGAERFAALVELSPVLV
                     VGIGGIAPENARAVLEAGAHGVAMMRSISEAPDVEAAARAFQLSLRGG"
     gene            complement(3071..>3373)
                     /locus_tag="PK513_10205"
     CDS             complement(3071..>3373)
                     /locus_tag="PK513_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82861.1"
                     /translation="AQSAAQPHRTTGLPVGIYEHLYGDTNPDQSFDRLHNQFCGRMRQ
                     KRALHERGQRQKGGCLTLRDRFNFGLDWLHWRLESLNSSSVRKPDLDLQATYIDPD"
BASE COUNT          857 a         1083 c          707 g          726 t
ORIGIN      
        1 aaatatggag cgttaaacat ccagaaccag acgcgcaggg tcttcaatcg cctgtttgat
       61 ccgcacgagg aaactgaccg cttcgcgccc atctataatc cggtgatcgt aagaatgggc
      121 cagatacatg atcggacgcg cctcaatagc cccattcggc ataaccatcg gacgctgcac
      181 aatattatgc atcccgagaa tcgccgactg cggcgcgttc aaaatcggcg tagacagcat
      241 cgaaccgaaa attccgccat tggtaatggt aaacgtcccg ccggtcatct cgtcaatcga
      301 caacttcccg tcacgcgccc gcacgcccag atcaacaatc ttggcctcaa tctcggccat
      361 gctcatctgg tccgcatcgc gtatcacagg cacaaccaaa ccctgcggcg ttgagacagc
      421 cacgccgata tcataataat ttttataaac gatgtctgta ccgtcaattt cggcatttac
      481 cgccgggaat tctttcagcg cattaatcgc cgccttcacg aagaacgaca taaagcccag
      541 cttaataccg tgcttcttta caaattggtc ctgataagct tttcgcagtt ccatcactgg
      601 gcccatatcc acctcgttaa aggtggtcag catcgccgca ttgttttgcg cctctttcaa
      661 acgcgttgcg atcacctgac gcagcttggt catacgcaca cgctcctcac gcgggcccgt
      721 ttcagtgcgc gggggatgag catctgaagc cggggcgggc gcacttggcg gtggactcaa
      781 tgacgcagcc ttcgccccct tgttatcagc aataaatttc tgcacatctt ctttgctcaa
      841 gcgctcacca ctggccggaa cttgcgccgc ctcaagattg ttatcagaca gcatcttgcg
      901 tacagaagga gacgtcttgg catctgctga agcttccggc gccgatgcga cctcttcctt
      961 cgcaggtgca ggagccgctt caacagtttt aggcgcttcc tctttcggag cctcatcttt
     1021 tgccggagcc gcaggcgtat cattggcagc cgccccctca cccatctcac cgagcaaagc
     1081 tccaacctca acggtttcgc cttcacccac agtaatcttg acaatcacgc catcagccgg
     1141 agcattcact tccagcgtca ccttgtcagt ttccagctca acaatcggct catcggcctt
     1201 caccgcttcg ccttcttttt tcaaccattg cgcgacagtc gcttcgctaa cggattcacc
     1261 taatgtggga acaacaatct gggtcatgag tatcctgctc gtgtttataa atatgctctt
     1321 aaaaatctac gctgaggtcg taccaaaggc aagcatgaac accgataaat acagctaaaa
     1381 atgttgccag cctgttttgg aaaaagacat ttcatgacca tcctgatcaa aaaccttcac
     1441 acccgccaca gcctcaaaaa caccgaccac agacaacgcc acacccaaag cccccgcctg
     1501 tttttggaac gcttcaacat ctgaaggaga aacagccata gccaattcat aatcctcccc
     1561 ccatgtcaga aggtcttcgg aactggcctt tccatcccta agcgcagcgc gccccttatc
     1621 ggatagcgga attttatcga attccagccg cgcccccagc ccggaaacac tgcaaatatg
     1681 cccaagatca gccaaaattc catcagaaat atccaccgcg gccttcgcat atttccgcac
     1741 caaaggcgcc agcaccaccg gcggcgaagg catatgcaaa cggcttaaat acgcatcccc
     1801 cagtatgccg gtaatcacca ccacatcgcc ggcgcgcgca cccgatcgca aaacagccct
     1861 gcccgtcggc accaagccca tcgccgtaat cgaaacgctt aagccccccg cgcgcatccc
     1921 cgtcgtatcc ccgccggaca gcgcgatatt ataaagcgct tgatccgccg ccaaaccatc
     1981 ggcaaacccg gctagccaat cttcatccag atctttcggc aaagccagat tgagctgata
     2041 agccagagga tctgcaccgc tggacaccaa atctgaaacg ttagagcgca aacatttccg
     2101 ccccgcatca aacgcgctca tctcctcaac gaaatgatcc ccgcggttag aagtatcact
     2161 actcaccacc aattgatgcc cggccggaat gtccagcacc gcagtatcgt tcttcaaccc
     2221 gtccatcgac agcggcgcaa aatatttttc tatgagatca aattcagcca tttctactcc
     2281 tgtcattgcg agcgcgaagc aaatgcatag catttgttcg catttttata aacaaacaaa
     2341 cctgcgattt tctttgcgcg aagcaatcca gaaaaacaat tctatagatt gccccgtcgg
     2401 ctttcagcct cctcgcaatg acaattgaaa cgcccgcgca gccgcttcca cgtccggcgc
     2461 ttcactgatg ctgcgcatca tcgccacgcc gtgcgccccg gcctccagaa cggcccgcgc
     2521 attttccggc gcaatcccgc cgatccccac aacaagcaca ggcgataatt caaccagcgc
     2581 cgcaaacctt tccgctccca aaaccggctt gcccttatca gtttgggttt cataaaacgg
     2641 ccccgtgccg acataatcaa ccacgcccgg atcaatcgcc gccatatgtt cgggtgtaaa
     2701 cgccgtctgg ccgataattg ccgccgcgcc cagtttcgcc cgcgcctctt ccggcgaact
     2761 gtccccctgc cccagatgca ccccagccgc acccacagcc tgagccacat cgatatgatc
     2821 attgataata aaagcaacgc ccggcgtcat ccattcctgt aacatcgccg cctcagccag
     2881 aatatccggc atcggatcaa acttgttgcg atactgcacc atcgtaacgc ccccgcgcaa
     2941 agccgcagcc acgacatcaa caatatcccg ccccgcacat aacaacggat cagccacaaa
     3001 atagatggag gggttgaaag gtttcaccat ggtgtaagct ataataatag taagacacaa
     3061 acagcagaac ttaatctgga tcgatatatg tcgcttgcaa atccaagtcc ggttttctga
     3121 cagaagaaga gttaagcgat tctaatcgcc agtgtaacca gtccaaacca aaattaaaac
     3181 ggtcgcgcaa agtaagacat ccaccttttt ggcgttgccc cctttcatga agggcacgct
     3241 tctgacgcat tcgaccacaa aattggttat gcaatcgatc aaacgattgg tcgggatttg
     3301 tatctccata aagatgttca tagataccca cgggtaaacc cgtggttcta tggggctgcg
     3361 ccgcgctttg cgc
//
LOCUS       DAOSWR010000182         3366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_20282_length_3366_cov_5.213531, whole genome shotgun sequence.
ACCESSION   DAOSWR010000182 DAOSWR010000000
VERSION     DAOSWR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20282_length_3366_cov_5.213531"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..397)
                     /locus_tag="PK513_10210"
     CDS             complement(68..397)
                     /locus_tag="PK513_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HOO82862.1"
                     /translation="MAKNRPYREFRAVEEEYFRNHPEEIETYIEEIFDEFAQDNNTAA
                     LLASLRVIAKVKGITNLAEEAQMTRQGIQHALSSKGNPRFDNVNAIMKAMGYRLMPEK
                     IDMVHAS"
     gene            complement(390..728)
                     /locus_tag="PK513_10215"
     CDS             complement(390..728)
                     /locus_tag="PK513_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO82863.1"
                     /translation="MAQPTEPKTVMVYQDENGKEPFTDWLNGLRDQKGRRAILKRIGR
                     LELGLYGDCEPVGEGVLELRIFLGPGYRVYFGEDGDNIVVLLSGGDKGSQDRDIKTAK
                     EFWKEYKDHG"
     gene            complement(906..1511)
                     /locus_tag="PK513_10220"
     CDS             complement(906..1511)
                     /locus_tag="PK513_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HOO82864.1"
                     /translation="MVLENKLNITNQVELAKAEEKLSKQKAKQLFESGDINKIEVGTF
                     TGLAKIHQYLFEDIYDFAGKVREVNIAKGNFRFAPLMYLRQSLDHINAMPQGNFDEIV
                     EKYVEMNIAHPFREGNGRATRIWLDLIFKKEIGKVVDWNRVDKDAYLSAMERSVVKDV
                     EIKALLKDAMTDKINDRETYMKGIDVSYYYEGYSEFKTEEL"
     gene            complement(1571..1771)
                     /locus_tag="PK513_10225"
     CDS             complement(1571..1771)
                     /locus_tag="PK513_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO82865.1"
                     /translation="MERNVRLNWQGFVEEAVRRRKEQKLTQEQLSVLAGVSKPTLNSF
                     EQGKTTIKLDSALKILKVLGLA"
     gene            complement(2111..2296)
                     /locus_tag="PK513_10230"
     CDS             complement(2111..2296)
                     /locus_tag="PK513_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82866.1"
                     /translation="MQDIIEKHQPDLWIYGHTHECDDQAIGKTRIISNQLGYPNRHGG
                     FECAGFDERGKEIEIKK"
     gene            2273..2554
                     /locus_tag="PK513_10235"
     CDS             2273..2554
                     /locus_tag="PK513_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO82867.1"
                     /translation="MLFDDVLHNLLFLRKVLKNLGFSGVDEAASGRKAIELAQQNTYD
                     LIFMDCQMPEIDGFEAATMIREREEKIGDIKIIAVTADAIANTARRSFF"
     gene            complement(2586..2738)
                     /locus_tag="PK513_10240"
                     /pseudo
     CDS             complement(2586..2738)
                     /locus_tag="PK513_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2742..2990)
                     /locus_tag="PK513_10245"
                     /pseudo
     CDS             complement(2742..2990)
                     /locus_tag="PK513_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013842.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(3023..>3366)
                     /locus_tag="PK513_10250"
     CDS             complement(3023..>3366)
                     /locus_tag="PK513_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOO82868.1"
                     /translation="AANSINIKDLKPEELLLLEDGHCLRDHALSACDLQLPKTRKTFS
                     ATSLPTLIQMVKAGYGITLLPEMACTPETIPPGIRLLPFNDKHPPTRQIGLCWRRGDP
                     RARDYEMLAKV"
BASE COUNT          770 a          835 c          798 g          963 t
ORIGIN      
        1 ttcggtcata gatcgtagct taactcactg tccgaattct tgggaccacc tctaattgaa
       61 ccggctttta ggaagcgtgc accatatcga ttttttcagg catcaggcga tagcccatgg
      121 ctttcataat ggcatttaca ttatcaaagc gaggattgcc cttgctggac aaagcatgct
      181 ggataccttg gcgcgtcatc tgcgcctctt cagccaaatt cgtgatgcct ttgacttttg
      241 caatcacgcg caaagaggca agtaacgctg ctgtgttgtt gtcctgcgcg aactcgtcaa
      301 aaatttcttc gatgtaggtt tcgatctctt cagggtggtt gcggaagtat tcttcctcaa
      361 cggctctgaa ttctctgtat ggtctgttct tagccatgat ccttgtactc cttccaaaat
      421 tcctttgcgg ttttgatgtc cctgtcctgg gagcctttat cgccacctga gagcaacacc
      481 acgatattgt cgccgtcctc tccaaaatag acccggtatc caggcccaag gaatattcgc
      541 aattctaaaa cgccttcgcc gaccggctca caatcgccat acaatcccag ctccagcctt
      601 ccaatccgtt tcagaatggc acgccgccct ttttgatcgc gaaggccgtt cagccagtcg
      661 gtaaacggtt ctttaccgtt ttcatcctga tagaccatga ctgtcttcgg ctcagtcggt
      721 tgtgccatat tcctatcctt ttaactatag catagccaaa tatatttggc aataaaaaac
      781 gccaaatgta tttggcagca atatattgtc cattgattct aatggcattt tcaatccctt
      841 cctaaaatca tgacagcttc agtaccttca aaatcttgag agtgctattg agcttgattg
      901 cggtcttaca gctcctcggt cttgaattcg ctgtaaccct cgtagtaata gctgacgtca
      961 atgcctttca tataagtttc gcggtcattg attttgtcgg tcatggcatc ttttaacagg
     1021 gctttgattt ccacatcctt gacaacgctg cgctccatcg ccgagagata cgcatcttta
     1081 tcaacgcggt tccaatccac gaccttgccg atttcttttt taaagatcag atccagccag
     1141 atgcgtgttg cacgcccgtt gccttcgcgg aagggatgcg cgatattcat ttcaacgtat
     1201 ttttcgacga tttcatcaaa gttgccctgt ggcatggcgt tgatatgatc cagagactgg
     1261 cgcaagtaca tcaggggtgc aaaacggaaa ttacccttgg cgatattgac ctcacgaacc
     1321 ttaccggcaa aatcatagat gtcttcgaac agatattggt gaattttggc aaggccagta
     1381 aatgttccga cctcaatctt gttgatgtca ccggattcaa aaagctgttt ggctttttgt
     1441 ttgctgagtt tttcctcggc ctttgccaac tcaacctgat ttgtgatgtt cagtttattt
     1501 tctaaaacca ttttattcct atccttctaa ctctatcata gcccaatata tttagtaaca
     1561 caccactgac tcacgccaga cccagcacct tcaaaatttt gagagcgcta tcgagcttga
     1621 tggttgtctt gccttgttcg aagctgttga gcgtgggctt gctgacgcct gccagaaccg
     1681 acagttgctc ctgcgtgagc ttttgctcct tgcggcgtct tactgcttct tcgacaaagc
     1741 cttgccaatt aagacgaacg ttccgttcca tcgctttcct atcagtttaa tgagattgtc
     1801 cttgaaatca gccccaccga gtggcgcctc ttggattgcg tccttggcgg cctcgatgtg
     1861 cttctccagc ccctgtacgg ccatgttgat ggcggctgac cgccatcgat atagctgtta
     1921 tcgtaggtga aagtgacacg gcctcctggc tcctcacgca aatagcccgc gaagatgtcc
     1981 ttgtaataga ccttacccca taataaattt gccacggtta aatcctttta actcaaaagc
     2041 ccttatcggg ctataaagta aaatataact tacttttgtt aatttttcaa caattaaagt
     2101 aaagcacatt ttacttttta atctcaatct ccttgcccct ctcatcaaac ccggcgcatt
     2161 cgaagccgcc atgccgattg gggtagccca gctggtttga aataatccgg gttttgccga
     2221 tggcctgatc atcgcattca tgcgtgtggc cgtaaatcca gagatccggc tggtgctttt
     2281 cgatgatgtc ctgcataacc tgctgttcct gcgcaaggtt cttaaaaatc tcggcttttc
     2341 cggggtggat gaggcggcaa gcggcaggaa ggccatagag ctggcgcagc aaaatacata
     2401 cgatttgatt ttcatggatt gccagatgcc ggagattgac gggtttgaag ccgctaccat
     2461 gatccgggag cgcgaggaaa aaatcggtga tataaaaatc attgccgtca ccgccgacgc
     2521 aatagccaat acagcaagaa ggagtttttt ctgatcatga tgacttccgg cagggggtgt
     2581 atagcctacg ccacataaaa tggttcaatt ttgaaaagcg cgtcaataga gcagaagagt
     2641 tgttttctga tggctttggt ttgagcccat ttttgttcga tggggttgag gtctggagag
     2701 taaggcggca agaaaagcag ggcatgaccg accccttttt cgttgacccc cagcctgcgc
     2761 tcccgttcgt actggtaagc atcaggatgg gccaaaacat ctctggccag ggccttcatg
     2821 tcgatcttgg ttgcgggctt gttgcgggac atcttcggtt ccgggtgttt aagccaccgt
     2881 acaacactgg ccacacccac acaaaaccgg gacgccgtct cggctatcgt aaggccttcc
     2941 ttctcgctga cagataagac tttacggcgg aaatcggatg aatacgtcat ccatgaagtg
     3001 taatcaattt atatgagttt gactatactt ttgcgagcat ctcataatcg cgggcgcggg
     3061 ggtcgccgcg gcgccagcac aggccgattt gccttgtagg cgggtgtttg tcgttaaagg
     3121 ggagcagacg gatgcccggc ggaattgttt cgggcgtaca ggccatttcc ggcaggaggg
     3181 tgatgccgta gcccgctttg accatttgga tcagggtggg cagtgaggtt gccgaaaagg
     3241 ttttgcgggt tttgggaagt tgaaggtcgc aggcggacag ggcatggtcg cgcaggcagt
     3301 gaccgtcttc gagtaagagt agctcttctg gcttcaagtc tttgatattg attgaatttg
     3361 cggctg
//
LOCUS       DAOSWR010000183         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_20535_length_3337_cov_4.981414, whole genome shotgun sequence.
ACCESSION   DAOSWR010000183 DAOSWR010000000
VERSION     DAOSWR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20535_length_3337_cov_4.981414"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PK513_10255"
     CDS             <1..1737
                     /locus_tag="PK513_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO82869.1"
                     /translation="PIQQFDRTVRTTLSRLGMSPAQAYQLGYIKELLNGEIRASLIQQ
                     KAADTGIQVSTQQVAANIKKLIAPMTQPGQSPQDVLAQILRAQGMSEDQLTASIRREM
                     SVNMLGNAVQSGFLKTSETMTRDLAAYENEQRNVDYILFKDADLKEIEQPADEQLLKL
                     YESTKEAYAIPEMRKSKLITIKMDTLKDSLEISDEEIKDVYERNISSYTQPETREIEQ
                     VILSDADQAEDIAKKIEAGASLKDATKEVTGNTTDYIPATSVEKTALLEELQEPVFTA
                     KEGDLLGPIESGLGYHVAMLITITPAHTTSLETAKKDIRTELTETRLLDAQYDLAASL
                     DDYLAASEPIETIKENLSVEIKDLPATNRFGIGENDKSAFNEEFGPDAQNMVSILFEL
                     GEGEASPVTELTDGRMSALLVENITEKSYKPFEDQKKALKKRWIDDSRRVQNKMHTLK
                     LLTTAQGDGTSIKDLAKAKGRTLSSAKDLKRASEIEQPLTKAALGQIFEAKQNELFAL
                     DLDEGAAIMVVTNTTIPEKLKDEELKAAQNTHLQEMQNEAYTLYINSLRKKYGVRVNE
                     KLLQMVYAPQDN"
     gene            1741..3252
                     /gene="trpE"
                     /locus_tag="PK513_10260"
     CDS             1741..3252
                     /gene="trpE"
                     /locus_tag="PK513_10260"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOO82870.1"
                     /translation="MNQSQPALSEFEHHFTAGRSQLLTHELPGDLETPVGTYLKLCMD
                     APYSFLLESVEGGNVLGRYSIIGMAPDLLWSCKKGSIEIKNANGAPIKDDAPDALASL
                     RAQVQACHIDEIPDGLPPMAASGLFGYIGYDMIRLIEDIPDNNPDDLQIPDSVLMRPS
                     ILVIFDNVKQIIILVTPVYEHANNTDKDARTIYKQAAKRLNQTIEQIFTYVDRNRLKS
                     RSNIETPLDVQSNMTREEYHDAVRKAVNYTFEGDIFQVVPSQRFSTDFDLPSFELYRS
                     LRRVNPSPFMFHVALGDFQLVGASPEILVRVRDNEITIRPIAGTRKRGKDTTEDKALA
                     EELLADTKERSEHLMLLDLGRNDVGRVAEFGSVKVTDSFFVELYSHVMHIVSNVTGKL
                     RKDLDIVDAMFAGFPAGTVSGAPKVRAMEIIDELEVSRRGFYAGGVGYLSGNGMLDTC
                     IALRTALVKDGKLYVQAGGGVVADSDPEFEYQETVNKSRAIIHAAELAIENAK"
BASE COUNT          983 a          946 c          720 g          688 t
ORIGIN      
        1 cctatccagc aatttgaccg cacagtgcgc acaaccctca gccgtttagg catgtccccg
       61 gctcaggctt atcaactggg ctatattaaa gaactattga atggagaaat tcgcgccagc
      121 ctcatccagc aaaaagccgc cgatacaggt attcaggtca gcacacagca agtcgccgcc
      181 aatatcaaaa aactcatcgc ccctatgaca caacccggtc aaagcccgca agacgtcctt
      241 gctcaaattt tgcgcgccca aggcatgagc gaagaccagc tcaccgcttc tattcgtcgt
      301 gaaatgagcg tcaatatgct gggcaatgct gtacaatccg gctttctcaa aacttcagag
      361 acaatgaccc gcgatcttgc agcttatgaa aacgaacaac gcaatgttga ttatatcctg
      421 ttcaaagatg ccgatctcaa agaaattgaa caacccgcag acgaacagct tctaaaactc
      481 tatgaaagca caaaggaagc ctacgctatc cccgaaatgc gcaaaagcaa gctcatcacc
      541 attaaaatgg acactttgaa agacagcctt gaaatttccg atgaagaaat caaagatgtc
      601 tatgagcgta acatctcttc ttatactcag cctgaaacac gcgaaatcga acaggtaatc
      661 ctttctgatg ccgatcaagc cgaagatata gccaaaaaaa ttgaagctgg agcctccctt
      721 aaagacgcaa caaaagaggt caccggtaac acaactgact acatcccggc tacgtcagta
      781 gaaaaaaccg ccctgcttga agaactccaa gaaccggttt ttaccgcaaa agaaggggac
      841 ctgctcggcc ccattgaaag cggcttgggt tatcacgttg cgatgctcat aaccatcacc
      901 cctgctcata caacttcact tgaaactgca aaaaaagaca tccgcacaga gcttacagaa
      961 acccgcctgc tcgatgcaca atatgatctg gcagccagcc tcgatgatta tctggctgct
     1021 agcgaaccca tcgaaacgat caaggaaaat ttgagcgttg aaattaagga cctgcccgcc
     1081 accaatcggt ttggcattgg tgaaaacgac aagagcgcct tcaacgaaga attcggcccc
     1141 gatgcgcaaa atatggtctc gattttattt gaactcggcg aaggggaagc ctctcccgtg
     1201 acagaattaa cagatggtcg tatgagcgcc ctgttggttg aaaacatcac cgaaaaatca
     1261 tacaagccct tcgaagacca aaaaaaagcg ctcaaaaaac gctggatcga cgactcccgc
     1321 cgcgttcaaa ataaaatgca tacacttaaa ttactcacca cagcccaggg tgacggcaca
     1381 tccatcaagg atcttgccaa agctaaaggc cgcacattga gcagcgccaa agaccttaaa
     1441 cgcgccagcg aaatcgaaca gccgctaacc aaagccgccc ttggccagat cttcgaagca
     1501 aagcaaaatg aactctttgc tctcgatctt gatgaaggcg cggcaatcat ggtcgtaaca
     1561 aacaccacca ttccggaaaa gctcaaagac gaagagctaa aggctgcaca aaacacacac
     1621 ctgcaagaaa tgcaaaacga agcctatacg ctttacatta atagcttgcg taaaaaatac
     1681 ggtgtacgtg tcaatgaaaa actcctccag atggtttacg ccccgcagga caattaataa
     1741 atgaaccaaa gccaacccgc actaagcgag tttgagcacc acttcacagc tggacgctct
     1801 caacttctca cccatgaact ccccggcgac ttggaaacgc ctgtaggcac atatctaaaa
     1861 ctctgtatgg atgcccccta ttcctttttg ctggaatcgg tcgaaggcgg caacgtactg
     1921 ggacgttact ccatcatcgg catggcccct gacttgctat ggtcatgcaa aaaaggaagc
     1981 attgaaatca agaacgccaa cggcgcgccc attaaagacg atgcccccga tgcactggcc
     2041 tcactacgcg cacaggttca ggcttgccat attgatgaaa tccccgatgg tttacccccc
     2101 atggccgcat ccggcctgtt cggctatatc ggctatgaca tgatccgtct gatcgaggat
     2161 attccagaca acaaccctga cgatctgcaa atccccgaca gcgtccttat gcgcccgtcc
     2221 atactggtga tttttgataa tgtgaagcaa atcatcatcc tggtcacacc cgtttatgaa
     2281 catgcaaaca acacagacaa agacgcccgg acaatttata agcaagccgc aaagcgtctg
     2341 aatcaaacaa tcgagcaaat ctttacatac gtcgatcgca accgcctcaa atccagatca
     2401 aacattgaaa cgcctctgga tgtgcaatcc aacatgactc gtgaagagta tcacgatgcc
     2461 gtgcgcaaag cggtgaacta cacatttgaa ggcgatattt ttcaggtcgt ccccagccaa
     2521 cgcttttcca ccgatttcga cctgccgtct ttcgagcttt atcgctccct gcgccgggta
     2581 aacccctcgc cttttatgtt ccatgtagcc cttggcgatt tccagcttgt cggcgccagc
     2641 cccgaaattc tcgtacgtgt acgcgataac gaaatcacta tccgccccat cgccggcacc
     2701 cgcaaacggg gcaaagatac cacggaagat aaggcattgg ctgaagagct cctcgccgat
     2761 accaaagaac gatccgaaca tttgatgctg cttgatctgg gccgcaatga tgtggggcgc
     2821 gtcgccgaat ttggcagcgt caaagtcacc gactcattct tcgttgaact ctactcccac
     2881 gtcatgcata tcgtctccaa cgtcaccgga aagctgcgca aagatctcga tattgtcgac
     2941 gctatgtttg caggcttccc cgccggaacc gtcagcggcg cacccaaagt tcgcgccatg
     3001 gaaatcatcg acgaactgga agtcagccgc cggggttttt acgcaggcgg cgttggttat
     3061 ctatccggca acggcatgct tgatacctgc atcgccctgc gcacagcgct tgttaaggat
     3121 ggaaagctat acgtacaagc cgggggaggc gtcgtcgccg acagcgaccc ggaattcgaa
     3181 tatcaggaaa ccgtcaataa atcccgcgct attatccacg ctgccgaact ggccatagaa
     3241 aacgcgaagt aagaagccgc cgccacttct tgcaatgaca aaaaagatag gcttaaggcg
     3301 cctgtcccag cattttcttg agaatagcct cacgcgc
//
LOCUS       DAOSWR010000184         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_22677_length_3135_cov_4.399675, whole genome shotgun sequence.
ACCESSION   DAOSWR010000184 DAOSWR010000000
VERSION     DAOSWR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22677_length_3135_cov_4.399675"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /gene="pheT"
                     /locus_tag="PK513_10265"
     CDS             <1..1792
                     /gene="pheT"
                     /locus_tag="PK513_10265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO82871.1"
                     /translation="AVKIDDREGCPQFLGRQIKGVKNGPSPDWLQKQLKAVGLRPISA
                     LVDITNFICMDLGRPLHAYDGDKLKGNIVVGTSKGGEELDALNDKSYELASGAVTIND
                     ESGVIGLGGIVGGTSTGCDEGTTNVFLEVAYFKPERIARTGRALGIESDARYRFERGI
                     DPAFLPEAMEIFTAMVLEICGGQASEVVAAGEQPDWQRTIEYDPAYCEQLIGLNVPQE
                     QQAEILEALGFEVSGKASALKITPPSWRGDVFGRADIAEEIARIIGFDAIPSESVQGL
                     VVPAAAETVLGAAMRKSRAALSARGLNECVTWSFLGREQAEAFGANDNATSALTLQNP
                     ISSEMNVMRPSILPNLIEAAQRNEARGYPDAALCEIGPVFAGVKPEDQNIVACGIRTG
                     ANAVRHWSDATSARRVDLYDAKADAIAALEACGAPGENAQITADAPAYFHPGRSGVLR
                     LGKNVMATFGEIHPGILEDMGIKFPVVGFEVMLNNIPQAKQKGTEKPMLNLEPLQPLT
                     RDFAFLVDDGVKAADLIRAAMAADKTLISDASIFDIYTGKGVEDDKKSVALSITIQPK
                     GETLTDKDLEAMMQKVIYLVAQKCGGVLRG"
     gene            complement(1811..2182)
                     /locus_tag="PK513_10270"
     CDS             complement(1811..2182)
                     /locus_tag="PK513_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO82872.1"
                     /translation="MRPRTSFLAARMPDSFLEATQLWQGTCNAAVFNDWLERQLCALL
                     NDNCVVVMDNATFHKSNKTKTLIEGKGAVLLFLPPYSPDLNPIEQDFGALKTSGSTIM
                     KNPSTKLLKRIIKYSNDYKMP"
     gene            complement(2236..2481)
                     /locus_tag="PK513_10275"
     CDS             complement(2236..2481)
                     /locus_tag="PK513_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82873.1"
                     /translation="MKTRVYSASFFLLLAIAPGLCACQSLRAPSMSGDPAKMSSDMLC
                     YRAQTTGKAELKAEAARRNLDCRAEIENDPLLTNQRY"
     gene            complement(2635..2979)
                     /locus_tag="PK513_10280"
     CDS             complement(2635..2979)
                     /locus_tag="PK513_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOO82874.1"
                     /translation="MTTSLPPCPKCSSPYTYEDGALLVCPECAHEWSAAGEETEIIRD
                     SVGNELQSGDTVTIAKDLKVGNNTIKVGTKVKNIRVLPREEWIGDHDIDARVPGFGPM
                     GLKSSVVKKVAD"
BASE COUNT          705 a          768 c          907 g          755 t
ORIGIN      
        1 cgctgtaaaa attgatgatc gtgaaggctg cccgcaattt ctgggccgcc agatcaaagg
       61 cgtcaaaaac ggcccgtctc ccgactggct gcaaaagcag ctgaaagccg taggcctgcg
      121 cccgatttcc gcgctggtcg atattaccaa ctttatctgt atggatcttg gacggccttt
      181 gcatgcctat gacggcgata aacttaaggg taatatcgtg gtcggcacgt ctaaaggcgg
      241 cgaggaattg gatgcgctta atgataaatc ctatgagctt gcttccggcg cggttacgat
      301 taatgacgag tccggtgtca tcggactggg cgggattgtc ggtggcacat ctacaggatg
      361 cgatgaaggc acgacaaatg tgttccttga agttgcgtat ttcaagcctg agcgcattgc
      421 ccgaacgggg cgggctctgg gcattgaaag cgatgcacgt tatcgtttcg aacgcggcat
      481 tgatccggcc tttttgccag aggcgatgga aattttcaca gcgatggtgc tggaaatttg
      541 cggcggacag gccagcgaag tcgttgcagc gggtgaacag cctgactggc aacgtacgat
      601 tgaatatgat ccggcttatt gcgagcagct catcgggctg aatgttcccc aagaacaaca
      661 agcagaaatc ctcgaagcgc tgggttttga ggtttccggc aaagcatctg cgctcaaaat
      721 cacgccgcca agctggcgcg gggatgtttt cggtcgcgcc gatattgcgg aagaaattgc
      781 gcgcattatt ggctttgatg cgatcccatc tgaatctgtc cagggcctag ttgtgcctgc
      841 ggcggctgaa actgttttgg gcgcagcaat gcgcaaaagc cgcgctgcat tgagcgcccg
      901 agggctaaac gaatgcgtca catggtcgtt tttaggccgc gagcaagccg aggcttttgg
      961 tgcgaatgac aatgctacct ccgcgctcac cttacaaaac cctatatctt cggaaatgaa
     1021 tgtgatgcgc ccgtcaatcc tgccgaattt gattgaagcg gcgcagcgta acgaagcgcg
     1081 cggatacccg gatgcagctc tgtgtgaaat cggaccagta ttcgcaggcg taaaacctga
     1141 agaccaaaac attgtggcct gcggcattcg caccggcgca aacgctgtgc gccattggtc
     1201 tgatgctacg tccgcacgcc gcgtcgattt atatgatgcc aaggccgacg cgattgcagc
     1261 gcttgaagcc tgcggcgccc ccggagaaaa tgctcaaatc actgccgatg cgcctgcata
     1321 tttccatccc ggtcgctccg gcgttttacg gctgggcaaa aacgtgatgg ccacatttgg
     1381 cgaaatccat cccggcattc ttgaggatat gggtataaag ttcccggttg tcggctttga
     1441 agtcatgctg aataacatcc cgcaagccaa acaaaaaggg accgaaaaac cgatgctgaa
     1501 tctggagcca ctccagccat tgacgcgtga ctttgcgttc ctggtcgatg acggggtaaa
     1561 agccgcggat ttgatccgcg ccgcgatggc agcggataaa accttaatca gcgatgcctc
     1621 gatcttcgac atttacaccg gcaagggcgt tgaggacgac aagaaatcgg ttgcactgtc
     1681 gatcaccatt cagcccaaag gcgaaaccct gaccgacaaa gaccttgagg ccatgatgca
     1741 aaaagtcatc tacctcgtag cacaaaaatg cggcggcgtt ttacgaggtt aattaaactc
     1801 ggatggaagg ctatggcatt ttatagtcat ttgaatattt aattatacgc ttcaataatt
     1861 tcgtcgatgg atttttcatg attgtactgc ctgatgtttt gagggctccg aagtcttgct
     1921 cgatagggtt gagatcggga gagtatggtg gcaggaagag caggacagct ccttttcctt
     1981 caatcaacgt tttggttttg tttgatttgt ggaaggttgc gttgtccatg acgacaacgc
     2041 agttatcgtt gagcagcgcg cagagctgcc tttccagcca gtcgttgaag acagccgcgt
     2101 tgcaggtccc ttgccagagc tgtgtggcct ccagaaagct atcgggcatt cttgccgcca
     2161 aaaacgatgt gcggggacgc atatgatcga gcattatttg tttcaaaagc cggggacgga
     2221 tttgcggatg aaggattaat agcgctgatt tgtcaggagc gggtcgtttt cgatttcagc
     2281 gcggcaatca agattgcgcc gggcggcttc ggctttgagt tcggccttac cggtggtttg
     2341 agcgcggtaa cacagcatat cgctgctcat cttcgccgga tcgccggaca ttgacggcgc
     2401 gcggagcgat tggcaagcgc aaaggcccgg cgcaatcgcc aaaagaagga aaaatgacgc
     2461 gctataaact cttgttttca tagcattttt gcctgatcct cctttttgtt cgccattttt
     2521 tgccggtggg ggcgggagtg tttattttaa accacaaaga ccacctggcg ccttgcttag
     2581 gccgtaaacc cgtagccatc tccgccgatt ttttagattt cgtcaagaaa aaatttaatc
     2641 agcgaccttc ttaacgacgg aggatttgag gcccatcggc ccgaagccgg gaacgcgcgc
     2701 gtcgatgtcg tggtcaccga tccattcttc cctgggcagg acacggatgt ttttcacctt
     2761 ggtgccgact tttatggtgt tgttgccgac cttaaggtcc ttggcgatgg tgacggtgtc
     2821 accgctttgc agttcgttgc cgacggaatc gcggatgatt tcggtttctt cgcccgcggc
     2881 ggaccattca tgcgcgcatt cggggcagac gagcagcgca ccgtcctcat aggtataagg
     2941 ggaggagcat tttgggcatg gggggagaga ggtggtcatg gggttatatt gtcatcccga
     3001 gcgcagcgaa gcaatccaaa atctagattg ccgcgtcggg ctaaagccct cctcgcaatg
     3061 acgagagggt tattcgcacc agtgtttgga ggtggggcag tcgaggtggt gtttggtttg
     3121 gcctgcgggg caggt
//
LOCUS       DAOSWR010000185         3056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_23566_length_3056_cov_5.227924, whole genome shotgun sequence.
ACCESSION   DAOSWR010000185 DAOSWR010000000
VERSION     DAOSWR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23566_length_3056_cov_5.227924"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK513_10285"
     CDS             complement(<1..185)
                     /locus_tag="PK513_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOO82875.1"
                     /translation="MTTENIALLKALGAKMDFLNQRQRVISQNIANTDTPGYRPKDLK
                     PVDFGNVLQNVTKGKSGV"
     gene            275..643
                     /locus_tag="PK513_10290"
     CDS             275..643
                     /locus_tag="PK513_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOO82876.1"
                     /translation="MVDEYDDGRKVSHKDRLTPLNIKKKSKRQTAVAVQEGEALGDAP
                     VIRAAGRGKLAEKILQLAFENDIKVREDSALAEMLAKIELDSPIPSEAFMAVAEILAY
                     VYQANGKPNPFNVSLEELED"
     gene            733..1023
                     /locus_tag="PK513_10295"
     CDS             733..1023
                     /locus_tag="PK513_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOO82877.1"
                     /translation="MDSVGGEGLALIKMLLALVFVLGLMGVLALVMKRLGLSGQGNMP
                     GTKRRLKVIETAPIDARHRMALIQRDDVQHLVIFGPNGETVVETGIAPPDND"
     gene            1151..1882
                     /gene="fliP"
                     /locus_tag="PK513_10300"
     CDS             1151..1882
                     /gene="fliP"
                     /locus_tag="PK513_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414654.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOO82878.1"
                     /translation="MTVVFFMPTEAFAQAINIDLGQDGDGTVTGRVVQLVVLLTVLSL
                     APSILIMMTSFTRIVVVLSFLRTAIGIQQTPPNTVMISLALFLTFFIMAPTLQAAYET
                     AVVPLINEEIDEVQAFERGVDPFKKFMITHVREQDLRLFMEMNKSEMPEDAMEVPLQV
                     LIPSFMISELRRAFEIGFMLFLPFLIIDLVTASILMSMGMMMLPPVTISLPFKIIFFV
                     LVDGWYLIAGSLIRSFDPANVPIPG"
     gene            complement(1869..2540)
                     /locus_tag="PK513_10305"
     CDS             complement(1869..2540)
                     /locus_tag="PK513_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82879.1"
                     /translation="MQEQCADIYEFSGIQIVSSFKELTEYEAWKKTPIAVLHRKLCGD
                     FNGLAQKFAGWLDIRAEERIQLMLSHQALNNNVCDVFDQIHPKLTDDQILAMEQILCD
                     MASTRHFETVLNIEGERYDPVTKNWHHDDGWKQGRILCAYNTIATEGIQNSDAEHQKD
                     EFYKRKGEARIFKINTGDMTRHAVKNTAADSNPFIHRRPKSAEDMGPRLLLQSIAGAG
                     WITLE"
     gene            complement(2594..>3056)
                     /locus_tag="PK513_10310"
     CDS             complement(2594..>3056)
                     /locus_tag="PK513_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82880.1"
                     /translation="SLLRARALSRKGRPDQALALLNELERTSDMNRLRADIAWTAGYW
                     DDAAEALDDVIVDQNISLTRPLDDENTALLLQRAVSLNLAGDRIALANMREKYSDLMA
                     QTEKAKVFEVITRPRQSGALADRETLLSVVSEVNLFEEFLNTYKNARPPSN"
BASE COUNT          696 a          673 c          847 g          840 t
ORIGIN      
        1 acgccggact tgcccttggt cacgttttgc agcacgttgc cgaaatctac gggtttaagg
       61 tctttggggc ggtaaccggg cgtatcggta ttggcgatgt tttgtgaaat tacgcgctgg
      121 cgctggttca aaaagtccat tttcgcgccc agggccttta aaagtgcaat attttccgtc
      181 gtcattgtct tataccttac ttatactcta tttatatggc cgcattttga gtttgcggct
      241 cttatgggtt taacaatgcg aaagccgtgc caagatggtt gatgagtatg atgatgggcg
      301 gaaagttagc cacaaagaca gactaacccc tttaaatata aagaaaaaat caaaacgtca
      361 gacggcggtg gcggtacagg aaggtgaggc gctgggtgat gcgccggtga ttcgcgcggc
      421 cgggcgggga aaacttgccg agaagattct gcagcttgcc tttgagaatg atattaaggt
      481 gcgcgaggat agcgcgctgg cggaaatgct ggcgaaaatt gagctggata gccctattcc
      541 gtcggaagcg tttatggcag tggcggagat tttggcctat gtttaccagg cgaatggaaa
      601 gccgaatccg tttaatgtgt cgctggaaga attagaggat tagaatgcac gaaaccccgg
      661 attcaatccg gggtccatgt gaattttgta tagaccccgt gttaaacacg gggtttcgaa
      721 ggagaaaaac ttatggatag tgtgggcgga gaaggtctgg ctttgataaa aatgctgctg
      781 gcgctggtgt ttgtgctggg gttgatgggc gtgctggcac tggtgatgaa gcggctgggc
      841 ttaagcggac agggtaatat gccggggaca aaacggcgat tgaaggtgat tgagaccgct
      901 cccatcgatg cgcgccaccg gatggcgctg atccagcgcg atgatgtgca acatctggtg
      961 atattcggac cgaatggcga aacggtggtt gaaacgggga tcgcgccgcc tgataatgat
     1021 taacatgttc tcccgattct taatgttatt gcgagcgaag caaagcaatc cagcaaaaaa
     1081 atggattgcc gcgtcaggcc ttgttttaaa agcaaacaaa gctgcgcttt gctttgcggc
     1141 gctcctcgca atgacggtgg tgttttttat gccaaccgag gcgtttgcgc aggcaattaa
     1201 tatcgatctg gggcaggatg gcgatgggac ggtaacgggt cgcgttgttc agcttgtcgt
     1261 tttgctgacg gttttgtctc tggcgccgtc aattttgatc atgatgacga gctttacacg
     1321 cattgtcgtg gtgctgtcgt tcctgcgcac agcaatcggg attcagcaaa cgccgcccaa
     1381 tacggtgatg atttcattgg cgttgttcct gacatttttc attatggctc ctaccctgca
     1441 ggccgcctat gaaacggctg tggtgccgtt gatcaatgaa gaaatcgatg aggtgcaagc
     1501 ctttgagcgc ggggtcgatc cgtttaaaaa gtttatgatc acgcatgtgc gcgagcagga
     1561 tttacggctg tttatggaga tgaacaagag tgagatgccg gaggacgcga tggaggtgcc
     1621 gttgcaggtg cttattccct ctttcatgat ttcggagctg cgccgcgcgt ttgagatcgg
     1681 ctttatgctg tttttgccgt ttttgattat cgatctggtg acggcgtcga tcctgatgag
     1741 tatggggatg atgatgttgc cgcccgtgac gatttccctg ccgtttaaga ttattttctt
     1801 tgtgctggtc gatggatggt atttgattgc cggatcgctg atccgcagtt ttgatccggc
     1861 gaatgtgcct attccagggt aatccacccc gcaccggcaa tactctgcaa taacaatcgc
     1921 ggccccatat cttcagcgct ttttggtctg cggtgaataa acgggtttga atctgccgcc
     1981 gtatttttaa ctgcgtgacg agtcatgtcg cctgtgttta ttttaaatat tcgcgcctca
     2041 ccttttcttt tgtagaattc atctttttga tgttctgcat cactgttttg aattccttct
     2101 gtggctattg tgttataagc acacaaaata cgtccctgct tccacccatc atcatgatgc
     2161 caatttttcg ttaccgggtc gtagcgttca ccttcaatat taagaactgt ttcgaaatgt
     2221 ctggtggagg ccatatcaca caatatttgt tccattgcca aaatttggtc atctgtgagt
     2281 ttagggtgga tttgatcgaa aacatcgcaa acattgttat ttagcgcttg atgagaaagc
     2341 ataagctgga tacgttcttc ggctctaata tccagccacc ccgcaaactt ttgagccaag
     2401 ccgttaaaat ctccacataa ctttctgtgc aaaacagcga taggcgtttt tttccacgct
     2461 tcatactccg tcagttcttt gaaagaacta acaatctgga tccccgaaaa ttcataaata
     2521 tcagcgcatt gctcttgcat ttcgcaccct caaataaaaa agcgaaaata cacaagctct
     2581 tcgattatgt caattaatta ctgggcgggc gcgcgttttt gtaggtgttg aggaattctt
     2641 cgaagagatt cacttcggaa accacagaga gcagggtttc gcggtcggcc agagcgccgg
     2701 actgacgtgg gcgggtgatg acctcaaaca ccttggcttt ttccgtttgt gccatcaggt
     2761 cggaatattt ctcgcgcata ttggccagcg ctatgcgatc accggccaga ttgagagaga
     2821 cggcgcgctg caagaggagc gcggtgtttt catcgtccag ggggcgggtc agggaaatat
     2881 tctggtcaac tatcacatca tccagcgctt cggcggcatc atcccaatag ccagcggtcc
     2941 aggcgatgtc ggcgcgcagg cggttcatat cggatgtgcg ttcaagctca ttgagcaggg
     3001 ctagcgcctg atcgggacgg cctttgcgcg acaatgcgcg ggcgcggagt aaggaa
//
LOCUS       DAOSWR010000186         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_26356_length_2851_cov_5.468884, whole genome shotgun sequence.
ACCESSION   DAOSWR010000186 DAOSWR010000000
VERSION     DAOSWR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26356_length_2851_cov_5.468884"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..369
                     /locus_tag="PK513_10315"
     CDS             103..369
                     /locus_tag="PK513_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82881.1"
                     /translation="MKDPERMADFILKHRKGWNEEDLAALVQDKKTRDLYISDPIPVY
                     LLYYTVWVNDQGGLVYGQDLYGFDDNLIKMLKDIDGIFIPVDNT"
     gene            533..1114
                     /locus_tag="PK513_10320"
     CDS             533..1114
                     /locus_tag="PK513_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOO82882.1"
                     /translation="MFENTGNVLGSSLNSIDRREILKLGLAGVLATVVPLGFGSDAQA
                     ARGTWRVGFRHAHTGESFNGVYRVGDKYLPEAFERLNYVLRDFRTGEVFPMDPRVIDI
                     ISLVQRKASQREPLEILSGYRSPKTNANLRRVSTGVAKNSFHMYGQAIDLHIKGYRTN
                     SLRKIAMNLRAGGVGYYPKSDFIHVDTGKVRSW"
     gene            1180..1680
                     /gene="folK"
                     /locus_tag="PK513_10325"
     CDS             1180..1680
                     /gene="folK"
                     /locus_tag="PK513_10325"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOO82883.1"
                     /translation="MILIGLGANLPSRYGMPEETLGAAVAALATVGLEIAKVSSVWRS
                     APVPVSEQPWYCNAVVVVETALSAKVLMGVLQGVEHDFGRVRAERNAPRVLDLDLLAY
                     GDEVCVDSVCTVPHPRMHQRAFVLKPLQEIAPAWRHPVFGLSVGDLVEKMPEGQEIEI
                     SQILAA"
     gene            1686..2531
                     /gene="folP"
                     /locus_tag="PK513_10330"
     CDS             1686..2531
                     /gene="folP"
                     /locus_tag="PK513_10330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO82884.1"
                     /translation="MTRKCADINVSNLHPFAGGPHIMGVVNVTPDSFSDGGKFFNSDK
                     AIAHGLQMLSEGATILDVGGESSRPGAEAIDTEEEISRVVPVIEGLLGKAPWVSIDTR
                     NAKTMEAALNAGANIINDISALSYDPDSLSVAAEAGVPIFLMHMQGTPQIMQKNPLYS
                     NVVKDVSQYLQQRIELCETHRIDVEMLVCDPGIGFGKTLEHNLLLLRNIKEFHGLGVP
                     ILLGTSRKSFIGKISNDEPPDDRLAGSLASALYGYSQGVQIFRVHDVAAHVQAFKVFD
                     TIQNV"
     gene            complement(2543..>2851)
                     /locus_tag="PK513_10335"
     CDS             complement(2543..>2851)
                     /locus_tag="PK513_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO82885.1"
                     /translation="FDGWPDVPNGETHIDFFERVKRGKTKALTHSDDPVLIVCHGGVM
                     RAFGEIHGIPTPGKFQNAHLYEFIPKPENLKFPWAVYDYRLCPKLKTLLKSESTIYQI
                     "
BASE COUNT          661 a          491 c          809 g          890 t
ORIGIN      
        1 cgggttttga tgcccaatgc ctataatatt tatctgcatg acacgaatga gcggcattaa
       61 tttgagcggg cgaatcgcgc ggcttcttcg gggtgtgtgc ggatgaaaga cccagagcgg
      121 atggcggatt ttatccttaa gcatcgcaag ggttggaatg aagaggactt ggcggcttta
      181 gtgcaggata aaaagacgcg cgatctttat atttcagatc caatccctgt ttatctgctg
      241 tactatactg tgtgggttaa tgatcagggt gggcttgttt atggtcagga tttgtatggt
      301 tttgacgata atttaataaa aatgcttaag gatattgacg gaatctttat tcctgtggat
      361 aatacttgag gttaggaacg gttttttgcg ccttttcggg tgtgtgaagc attttatgga
      421 gaagcaggct ggttttcttt ggttttggcc tttttctcgt tcgaaaattc tgttagttct
      481 ttcaagttaa agttttaaaa gtcttataac aataattcat caaaggtggc gaatgtttga
      541 aaacactgga aatgtgcttg gttcgagttt gaactctatt gatcgtcgtg aaattttgaa
      601 gcttggtctg gctggagttt tggcaaccgt tgtgcctttg gggtttggtt cggatgcgca
      661 ggctgctcgc ggaacttggc gcgttggttt tcgccacgct catacgggtg aaagttttaa
      721 tggtgtttat cgggttggtg ataaatattt gcctgaagcg tttgagcgtt tgaattatgt
      781 tttgcgtgat tttcgcactg gtgaagtgtt tccgatggac ccgcgggtga ttgatattat
      841 ttctctggtg cagcgtaagg cttctcaacg tgagcctttg gaaattttgt ctggttatcg
      901 cagtccgaag acaaatgcga atttgcgtcg ggtgagtacg ggtgtggcca agaattcttt
      961 tcatatgtac ggtcaggcga tcgatctgca tatcaagggt tatagaacaa atagcctgcg
     1021 taagattgcg atgaatttgc gtgcgggcgg ggttggttat tatcccaaaa gtgactttat
     1081 tcatgtagat actggcaaag tccgtagctg gtaatctctc tttatcattt attattaatg
     1141 agctagagtg cagccttctg attttacagg aggctgtttt tgattctgat tggattaggg
     1201 gcgaatttgc caagccgtta tggtatgccg gaggagactt taggggcggc tgtagcggca
     1261 ctggcgactg tcgggcttga gattgcgaaa gtgtctagtg tttggcgcag tgcgccggtg
     1321 ccggtgtctg agcagccgtg gtattgtaat gcggttgttg tggtggaaac ggctttgtct
     1381 gcgaaggttc ttatgggcgt cttgcagggt gttgagcatg attttggacg tgtgcgagct
     1441 gagcggaatg cgccacgagt tttggatctg gatttgctgg cctatggtga tgaggtgtgc
     1501 gtggattccg tgtgcacggt accgcatccg cgtatgcatc agagagcctt tgttctcaag
     1561 ccgttgcagg aaattgcgcc agcgtggcgg catccggttt tcggcttgtc tgtcggagat
     1621 ttggttgaaa aaatgcctga ggggcaggag attgaaattt cacaaatatt ggcggcgtga
     1681 tagaaatgac ccgaaaatgc gccgatataa atgtttcaaa tctgcaccct ttcgcgggcg
     1741 gtccgcatat tatgggtgta gtcaatgtga cccctgatag ttttagtgat ggtggtaaat
     1801 tttttaattc tgacaaggcg atagcacatg gtttgcagat gttgtctgag ggtgcgacga
     1861 ttctggatgt tggcggggag tcctcgaggc ctggtgcgga agctattgat acagaagaag
     1921 aaatttcgcg tgtggtgccg gttattgaag ggctgcttgg taaagcgccg tgggtcagta
     1981 ttgatacgcg caatgcgaaa actatggaag ccgcacttaa tgctggggca aatattataa
     2041 atgatattag tgccttaagc tatgatcctg atagcttgtc tgtggcagcg gaggctggcg
     2101 tgccgatctt tttgatgcat atgcagggaa ctccgcagat tatgcagaaa aatcctttgt
     2161 atagcaatgt tgtaaaagat gtttctcagt atttacagca acgcattgaa ttatgtgaga
     2221 cgcaccgtat cgatgtagaa atgcttgttt gtgatccggg gattggattt ggcaaaacat
     2281 tagagcataa tctgttactg cttagaaata taaaagaatt tcatggtttg ggggtgccta
     2341 tcctgcttgg gacgagccgg aagagcttta ttggtaaaat ttcgaatgat gaaccgccag
     2401 atgaccgttt agcaggttca ttggcttcgg cactttatgg atattcgcaa ggcgtgcaaa
     2461 ttttccgcgt gcatgatgtg gctgcacacg tacaggcgtt taaagttttt gatacgattc
     2521 agaatgtgta aggtgtaaaa ttttagattt ggtaaatcgt ggattctgat tttagtaaag
     2581 tttttaattt tgggcaaaga cgataatcat aaaccgccca agggaatttt agattttccg
     2641 gcttaggtat aaattcatac aaatgggcgt tctggaattt tcctggtgtg gggatgccgt
     2701 ggatttcacc aaaagcgcgc ataaccccgc cgtggcatac aatcaatacc gggtcatctg
     2761 aatgagttaa agcctttgtt tttccccgtt tgacccgttc aaagaagtca atgtgcgttt
     2821 ctccattcgg cacgtcgggc cagccgtcaa a
//
LOCUS       DAOSWR010000187         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_27395_length_2782_cov_5.886322, whole genome shotgun sequence.
ACCESSION   DAOSWR010000187 DAOSWR010000000
VERSION     DAOSWR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27395_length_2782_cov_5.886322"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK513_10340"
     CDS             complement(<1..293)
                     /locus_tag="PK513_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82886.1"
                     /translation="MYTERGNVLWFILIAVALLAALTMVLSRSGSTVDQSGDIEQQRV
                     KASQILRTAKSIEAGIQQMRLRGVSENDMSFWHDSNGDNTEDGSDTYYNANCT"
     gene            complement(297..1490)
                     /locus_tag="PK513_10345"
     CDS             complement(297..1490)
                     /locus_tag="PK513_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOO82887.1"
                     /translation="MQNTKASPHIKPENIDVTEISAASHPQYMDHEKIYKIHAPEQNI
                     TAFVGIHNTALGPALGGIRFKYYEDENTALTDVLRLSEAMTWKNAAGNLDHGGGKSVI
                     MAPSADLRHPTDEILAIFAQGLNIINAGTPVYFGAEDMNTDETSMNKLLETTRWIKGG
                     TAPSPDVVGGTPSPLTAIGVFECMKIAARHKLGKENLKDVHISMQGLGNVGGALAQYL
                     HEQGAILTGCDVVDSVFEDLETKGVQVVRVGLNEIYDAPADIFAPNAIGGTLTDTNIK
                     RLKTADVQIVCGAANNQQQDQSGGHQSRLMHDLDMLYCPDYIVNAGGVIWVAKVGQDA
                     KTVTKEISTGVPRRFEEILQIYESATDQDMASLAARYARRRVETAQSQNSQNTGTETH
                     NMIGA"
     gene            2028..2714
                     /locus_tag="PK513_10350"
     CDS             2028..2714
                     /locus_tag="PK513_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82888.1"
                     /translation="MKKLVFVFSFLLIAFSPVVVWAEDKAEEVPKKNEVLEAADAFMK
                     DLDERSQRHFSIMYGNYNLIKVVESVRDHVGMAVEACGETNPDMKEPLETRFDEWKAA
                     IKPIMEEADANVGNMILAQEYAKPREIKKFFKLVDTVRKDKDKEVEKVPVTSPEACQT
                     LLENMDKTQPEMTRLLKTTLVSLPMVMQQQDQEQKEEEARVAAEKEAAEKEAAERAKA
                     EEEAGEAETR"
BASE COUNT          675 a          527 c          786 g          794 t
ORIGIN      
        1 atggtacagt tggcgttgta gtaagtgtcg gagccgtctt ctgtattgtc gccattgctg
       61 tcatgccaga aagacatatc gttttcagag acgccgcgca ggcgcatttg ctggattccg
      121 gcttcgatgc ttttggctgt gcgcaggatc tggctggctt tgacgcgttg ctgttcaata
      181 tcgccgcttt gatcgacggt tgatccgcta cggctgagca ccattgtgag cgcggcgagc
      241 agtgcgacgg cgatgagaat aaaccacagg acatttccgc gttcagtgta catgctttaa
      301 gctcctatca tgttgtgtgt ttcggtccca gtgttttgtg agttttgaga ttgggcggtt
      361 tcaacgcgcc gacgggcgta gcgggcggcg agggaggcca tgtcctggtc agtggcggat
      421 tcgtagattt gcaagatttc ttcgaagcgg cgcgggacgc cggtgctaat ctcttttgtt
      481 acagtttttg cgtcttgtcc gacttttgca acccagatga caccgcctgc attgacgata
      541 taatcagggc aataaagcat atccaggtcg tgcatgaggc ggctttggtg gccaccggat
      601 tgatcttgtt gctggttgtt ggcggcgccg cagacgattt gtacgtctgc ggttttgagg
      661 cgtttgatgt tggtgtccgt taatgtgcct ccaatggcgt tgggtgcgaa gatgtctgct
      721 ggtgcatcgt aaatttcatt aaggccgaca cgtacaacct gtacgccttt agtttcgaga
      781 tcttcaaata cggaatcgac gacgtcgcag cctgtaagaa ttgcgccttg ttcgtggaga
      841 tattgcgcga gtgcgccgcc tacattgcca aggccttgca ttgagatatg cacatcttta
      901 aggttttctt tgccgagctt gtggcgggct gcgattttca tgcattcgaa tacaccgatg
      961 gcggtcaggg gagagggcgt gccgccaacg acatcaggag agggcgcggt gccgccttta
     1021 atccatctgg ttgtttcgag cagtttattc atgctggttt catccgtgtt catgtcttca
     1081 gcaccgaaat agacgggtgt gcctgcgttg atgatgttga gcccttgcgc aaatatagcc
     1141 agtatttcat cggttgggtg gcgcagatcg gcagatgggg ccatgattac gcttttgccg
     1201 ccgccgtggt caaggtttcc ggcggcattt ttccaggtca tggcctcgga gaggcgtaaa
     1261 acatctgtca gcgccgtatt ttcatcttcg taatatttga agcggatgcc gcccaaggct
     1321 ggtccgagag ctgtgttgtg gatgccaaca aaagcggtga tattttgctc tggcgcgtgg
     1381 attttataga ttttttcatg gtccatatat tgggggtgag acgcagcaga aatttctgtg
     1441 acgtctatgt tttccggttt gatgtggggc gaagcttttg tattttgcat gattcttact
     1501 tttatataat tattttaatg ggttaagagg ccataaaatg gctttatgcg caagtaaaat
     1561 cctgaaaaat ctagaagagt agaacgtagg aaaatattac aaaattcatc tttaaaatta
     1621 aagaaattca tatcgaattt tatttacttt ctctttagat tttatatgtt atgttaaata
     1681 tatagggtaa gtgatcagta actccggtgt gatcctttcc ttcaattggt agaaagacca
     1741 gtgatttagg gacaaattgg cttcttttga gacgagaagc cggaattggg caagggaagg
     1801 cccgcttaat aaaaaattag ggttcgtgtc tgaaaacgtt cagacaaaag gttttaaaaa
     1861 tatttttggt ttatctctta tgtcatgcgc actggcctgt atgatgagtt tgggaattgt
     1921 gttgccccat tttattcccg gacaaaaaag catacaggct ttttttttgc ccgtttacgg
     1981 gctattttta tgtctgcaat attttaaaaa cagtgagcgc gtgatccatg aaaaagttag
     2041 tatttgtatt ttctttcctg cttattgcct ttagccccgt agtcgtttgg gccgaagata
     2101 aagcagaaga ggtgcctaag aagaatgaag ttttagaagc ggccgatgcg ttcatgaaag
     2161 atctggatga gcgaagccaa cggcatttta gtattatgta tggaaattat aatcttatca
     2221 aggtcgttga atcggtgcgt gatcatgtgg ggatggcggt ggaagcttgc ggtgagacta
     2281 acccggatat gaaagagcct ctggaaacgc gttttgatga atggaaagcg gcgattaagc
     2341 cgattatgga agaggctgat gcaaatgtcg gtaatatgat tttggcgcag gaatatgcga
     2401 agccgcgtga gattaagaaa ttttttaagc tggttgatac ggtgcgcaag gataaagaca
     2461 aggaggttga aaaagttccg gtgacgtcgc ctgaggcgtg tcagactttg ttggaaaata
     2521 tggataagac tcagcctgaa atgacccggc ttttgaaaac aactttggta agtttgccga
     2581 tggtcatgca gcagcaggat caggagcaaa aggaagaaga agccagagta gcggcggaga
     2641 aagaggctgc ggagaaagaa gcggcggaga gagcaaaggc ggaagaagaa gccggtgagg
     2701 ctgagaccag atgatagcca agaacagcgc cgtttgtaaa aaagccgtat acgcatgaga
     2761 atccttcaag ttatggccgg gg
//
LOCUS       DAOSWR010000188         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_27680_length_2765_cov_5.081550, whole genome shotgun sequence.
ACCESSION   DAOSWR010000188 DAOSWR010000000
VERSION     DAOSWR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27680_length_2765_cov_5.081550"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..512
                     /locus_tag="PK513_10355"
     CDS             117..512
                     /locus_tag="PK513_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HOO82889.1"
                     /translation="MGLLSFLGVLSPAHINFFTMTSGGEKVGSDQLGNIYYRAKPRLG
                     YKRERRWVMYNGVPEASNVPPEWHGWLHYQTDEIPEEGAESFRRPWQKPHQPNMTGTN
                     QAYRPPGHILEGGQRDKATGDYKPWMPPE"
     gene            541..1008
                     /gene="mlaD"
                     /locus_tag="PK513_10360"
     CDS             541..1008
                     /gene="mlaD"
                     /locus_tag="PK513_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOO82890.1"
                     /translation="MKRSVVETALGALVVIVAVSFLMFSYKLSNIGAGSDGYEVIANF
                     AGIGGLAAGDDVQISGVKVGTITAVELDEKTYLARVHIKVGNEVKLPLDTAALISSES
                     LLGGRYLALEPGADEEYLGPGGVIEYTQAPQNLEQLLGQFIFNQQGENAAPSQ"
     gene            989..1525
                     /locus_tag="PK513_10365"
     CDS             989..1525
                     /locus_tag="PK513_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HOO82891.1"
                     /translation="MPHLHNKAALLFLTGAVSLCVAALLISSRADAKPSIDYPIVKLR
                     SLDKITARTEVFEANVGRTIKFGSVYIKVQACRKAPELEKPEAAAFLQVWDAPPAAEG
                     EETEPEWIFSGWMFASSPGLSAMDHPIYDVWVLDCLKLENEREQPMIEEPEAFDEIRE
                     GEGVVSEEAAPDQPVIAQ"
     gene            complement(1526..2221)
                     /gene="aat"
                     /locus_tag="PK513_10370"
     CDS             complement(1526..2221)
                     /gene="aat"
                     /locus_tag="PK513_10370"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOO82892.1"
                     /translation="MQLVMTPELLIEAYRQGLFPMAYSGESAYVHWICPEERGQLPIE
                     DLHVSKSLKKTVLNNIKRNGPYKILIDTAFERVMRECAAAQEDRPETWINEPIIEVYC
                     ALHKRGMAHSVEVWEDEKLVGGVYGVSLGGAFFGESMFSRKSNTSKIALVHLCARLWK
                     AGYLIFDTQFVNDHLLQFGAYEVSHCDYIENLNAAVKKQTDFTLSDTPGLDEATLVNE
                     YFEMRKKHLPGEN"
     gene            2314..2649
                     /locus_tag="PK513_10375"
     CDS             2314..2649
                     /locus_tag="PK513_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82893.1"
                     /translation="MSNIRVFTLVAIAGGSALISGCVDDGTPVSRQCPPESEKQFLFT
                     FVDNSDASNVNPDRLEVTPMSGKEKGLPHIFNTQNMDVMKVQAVSEKLCAGRLKLEDV
                     NPADLAADL"
BASE COUNT          763 a          648 c          654 g          700 t
ORIGIN      
        1 aagtgtttgt aatttctctt gcaaattcat atggggggtg tccttttcat tttttcgtga
       61 tattaaattc tatcgagctt ttgtaaagaa aaacacaagc gaattatagg catattatgg
      121 gattattaag ctttctaggc gttttatcgc ccgcgcatat caacttcttt accatgacat
      181 caggtggaga aaaagtcgga agcgaccaac tcggcaatat atattaccgc gccaaaccgc
      241 gccttggcta taagcgtgaa cgccgctggg tcatgtataa tggtgtacct gaggcttcaa
      301 atgtgcctcc agaatggcat ggctggctgc attaccagac cgatgaaatt cccgaagagg
      361 gtgcagaaag tttccgccgt ccatggcaaa aaccgcacca gcctaacatg acaggcacta
      421 atcaagcgta tcgcccgccg ggtcatattc ttgaaggcgg acaaagagac aaggccacag
      481 gggattataa accctggatg cctcccgaat agatcgagtt gaaaagaaaa ggatagatgc
      541 atgaaacgct ccgtagttga gaccgccctt ggcgcattgg ttgtgattgt cgccgtgagt
      601 tttctgatgt tcagctataa actctccaat atcggcgcgg gcagcgacgg ttatgaagtg
      661 attgccaatt ttgctggcat cggcgggctt gccgctggcg atgacgtaca aatcagcggg
      721 gtaaaagtcg gcacaatcac cgctgtagaa cttgatgaaa aaacctatct ggcgcgtgtt
      781 cacatcaaag ttggaaatga agttaaactt ccgctcgata ccgcagcttt gatcagcagt
      841 gaaagtcttc tcggtgggcg ttatctagcg ctggaacctg gcgctgatga agaatatctc
      901 ggccccggcg gcgtcattga atatacgcag gctccacaaa atctggagca actgctaggc
      961 cagtttattt tcaaccagca aggtgaaaat gccgcacctt cacaataaag cagcgctgtt
     1021 attcctgacc ggagcagtgt cactgtgtgt agcggcgctg ctcatatcat cgagggccga
     1081 tgcaaagccc agcattgatt atccaattgt taaacttcga tcacttgata aaatcaccgc
     1141 ccgcacagag gtgtttgaag ccaatgtcgg gcgcacgatt aaattcggtt cagtctatat
     1201 taaggtccag gcctgccgta aagcgccaga gctggaaaaa ccggaggccg ccgctttcct
     1261 gcaggtctgg gatgctccac ctgctgctga aggggaggaa acagagccag aatggatttt
     1321 tagtggctgg atgtttgcgt cctcaccggg gctctcggct atggatcatc ctatctatga
     1381 tgtctgggta cttgattgtc tcaagctgga aaatgaacgt gagcaaccaa tgatagaaga
     1441 acctgaggct ttcgatgaaa ttcgtgaagg tgaaggtgta gtatctgaag aggctgcgcc
     1501 cgatcagccg gtaatcgcac agtaattaat tttcgccggg cagatgcttt ttgcgcattt
     1561 caaaatattc gttcaccagc gttgcctcat ccaagcccgg cgtatccgaa agcgtaaaat
     1621 cggtttgttt tttcaccgcc gcgttcaaat tctcaatata atcgcagtgt gaaacctcat
     1681 aggcgccaaa ttgtaacaaa tgatcattta caaactgcgt atcaaatatc agatagcccg
     1741 ccttccaaag ccgcgcacat aaatgaacca gtgcaatttt gctggtattg gacttgcggg
     1801 aaaacatact ttctccaaaa aaggcgccgc caagggaaac gccgtaaacc ccgccaacaa
     1861 gcttctcatc ttcccaaact tcaacgctat gggccatccc gcgcttatgc aatgcgcaat
     1921 agacttctat gataggctca ttgatccagg tttcaggacg atcttcctgt gccgctgcac
     1981 attcgcgcat aacacgctca aacgctgtat cgattaatat tttatacgga ccgtttcgtt
     2041 ttatgttatt aagaacagtt tttttgagag attttgaaac atgcagatcc tcaatcggca
     2101 attgcccgcg ctcttctggg caaatccaat gcacatacgc actctctccg ctataagcca
     2161 tcgggaataa tccctgccta taagcttcaa tcaacaattc cggcgtcatc acaagctgca
     2221 tagcgccatt atagcaaaag cacagctttt acaaaatatc ttgactgcac aaagagggtg
     2281 atatagaaaa taaacgtaat tatgaggatg aatatgtcaa atataagagt tttcacttta
     2341 gtagctatag ccgggggttc agcattaatc agtggctgtg tggatgatgg gacgcctgta
     2401 agccgtcaat gtccaccgga aagcgaaaaa cagttcctgt ttacctttgt tgataacagt
     2461 gatgcgtcga acgtcaatcc ggatcgcctg gaagtcacac caatgagcgg caaggagaag
     2521 gggcttcctc atatttttaa cacgcaaaac atggatgtaa tgaaggtaca agcagtatcg
     2581 gagaaacttt gtgctgggcg tttaaagcta gaagatgtta atcccgcaga cctggcagcc
     2641 gatctttagc gatctttaag ctgctcttcc aaccagtgaa tcgtataatc gccgctcaga
     2701 aaagcatctt accgtaaaat ccagtcatgc agcggcaggg tcgttttaat cccgctaaca
     2761 atggt
//
LOCUS       DAOSWR010000189         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_30219_length_2615_cov_5.477344, whole genome shotgun sequence.
ACCESSION   DAOSWR010000189 DAOSWR010000000
VERSION     DAOSWR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30219_length_2615_cov_5.477344"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK513_10380"
     CDS             complement(<1..327)
                     /locus_tag="PK513_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017051410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82894.1"
                     /translation="MYKVGDKITVNDKMQSGYSYRLDAPMGKNFDPAFKPHFTPKEML
                     ELGVFEGKYCNDCRDEFPDDWFENAKISERADASLNCFKIKSRQPLQVWQQKGWIAPP
                     DVRGWFQ"
     gene            complement(333..>2615)
                     /gene="pepN"
                     /locus_tag="PK513_10385"
     CDS             complement(333..>2615)
                     /gene="pepN"
                     /locus_tag="PK513_10385"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOO82895.1"
                     /translation="RLEGLYKSGDTYCTQCEAEGFRTITYYPDRPDVLTVFTVRVEAS
                     KAHYPVLLSNGNRVDHGDSDGGRHYAVWHDPFPKPCYLFALVAGDLTHIQDTYTTKSG
                     RAVELYIYVRPGDEDQCGHAMESLKKSMKWDEDTYGLEYELDMFNIVAVNDFNMGAME
                     NTSLNIFNTALVLAAQETATDQDFSRVESVIAHEYFHNWSGNRVTCRDWFQLSLKEGL
                     TVFRDQEFSADMNSRAVQRIDDVTHLRRLQFSEDAGPLAHPIRPDNYIEINNFYTMTV
                     YEKGAEVIRMLRMILGAENYRKGTDLYFSRHDGQAVTCDDFVACMEEASRIDLSQFKL
                     WYSQAGTPKIAFKGEYNPKNKTYSIELTQEIPDTPGQNNKKPMHIPVAIGLLGDNGQD
                     LIETQVLDLRDGQQRFVIEDVGQAPVPSILRGFSAPVRLENELSNADLTFLMLHDSDG
                     FNRWEAGQALILRMLDQMIDGDSKEICPKFMDTIGALIEDALDPVADKALMARALSVP
                     SVGLIGQQRAVIDPDGIHKARQALLAAIKRNYKPQLEKLYKANRSADAFSLSPEAMGR
                     RALRNVVLGLLSATNGTGCARLAKAHYETADNMTDRVAALARLADNQNAERDEAFDDF
                     YARFKAYPLVVDKYFALQAGAVRKDIIQNLNKLKQHSDFNIKNPNRVRSLYAAFAMNN
                     PIGFHAVDGSGYGFLCDEILELNTINPQIAARLLTPMREWRRYTEDRQTKMRSCLERI
                     VGTKDLSPDVYEIASKSLKG"
BASE COUNT          661 a          760 c          537 g          657 t
ORIGIN      
        1 ctgaaaccag ccgcgtacgt ctggcggggc aatccagccc ttttgttgcc agacttgcaa
       61 aggctgacgg ctcttgattt taaaacaatt cagagacgca tccgctcgtt cactgatttt
      121 tgcattctca aaccagtcat cggggaattc atcccggcaa tcattacaat attttccttc
      181 aaatacgccc agttcaagca tttctttggg cgtaaaatgc ggcttaaaag ccggatcaaa
      241 atttttaccc attggtgcat ccagccggta gctgtaaccg ctttgcattt tgtcattgac
      301 ggtaatttta tcgccaacct tgtacatcag gctcagccct ttagactttt gctggcgatt
      361 tcataaacat ctggagaaag atctttcgtt cccacaatcc gctccagaca gctacgcatt
      421 ttggtctgac gatcctccgt atagcgcctc cattcccgca tcggcgtcag cagccgcgcc
      481 gcgatttgtg gattaatcgt gttcaactcc aaaatctcat cacacaaaaa gccatatcct
      541 gatccatcca cagcatgaaa accgatcggg ttattcatcg caaaggccgc atagagtgac
      601 cgaactctat taggattttt tatattaaaa tcagaatgtt gtttaagttt attaagattt
      661 tgaataatat ctttgcggac cgccccagcc tgcaacgcaa agtacttatc cacaaccagt
      721 ggataagcct taaaccgcgc ataaaaatca tcaaacgcct catcacgctc agcgttttga
      781 ttatccgcca gccgagccag cgctgccacc cggtcggtca tattatcagc cgtctcataa
      841 tgcgccttgg ccagccttgc gcatcccgtt ccgttcgtcg cgctcagcag gccgagaaca
      901 acattacgca gcgcccggcg gcccattgcc tcaggcgaaa gcgaaaacgc gtccgcactt
      961 cggtttgctt tatacagctt ttccagctgc ggcttataat tccgtttaat tgcggccagt
     1021 aaagcctgac gcgccttatg aatgccgtcc ggatcaatca ccgcacgctg ctgtccgatc
     1081 aagccgaccg agggcacgct taacgcccgc gccatcaacg ctttatcagc caccgggtcc
     1141 agagcatcct caatcaacgc gccaatcgta tccataaatt tcggacaaat ctccttgctg
     1201 tccccatcaa tcatctggtc cagcattcgc aaaatcagcg cttgccccgc ctcccagcgg
     1261 ttaaacccgt cactgtcatg gagcatcaga aaagtcaaat cagcattgga aagttcgttc
     1321 tcaagacgta caggcgcaga aaacccacgc aaaatcgatg gcaccggggc ttgtccaaca
     1381 tcttcaataa caaagcgctg ttgtccgtca cgcaaatcta aaacctgcgt ttcaattaaa
     1441 tcctgaccat tgtcacccaa taagccaatc gccaccggaa tatgcatcgg ttttttattg
     1501 ttttgccccg gcgtatcggg aatttcctgc gttaattcta tactatatgt cttgtttttc
     1561 gggttatatt cacctttgaa ggcaattttc ggcgttccgg cttgcgaata ccatagctta
     1621 aattgcgata aatcaattct gcttgcctct tccatacagg caacaaaatc atcgcaggtc
     1681 accgcttgcc catcatggcg cgagaaatac aaatccgtac ccttacgata attctccgcc
     1741 cccaaaatca tccgcagcat gcggatgacc tcggcccctt tttcatacac ggtcatcgtg
     1801 tagaagttat tgatttcaat gtaattatcc ggccggattg gatgggcgag gggaccggca
     1861 tcctcagaaa actgcaaacg ccgcagatgc gttacatcgt caatccgctg cacggctcgt
     1921 gaattcatgt ccgccgaaaa ttcctgatcc cgaaaaaccg tcagcccttc tttgagtgac
     1981 aactggaacc agtcccggca agtcacgcgg ttcccgctcc aattgtggaa atattcatgc
     2041 gcaattacac tttccacgcg gctgaaatcc tgatccgtcg ctgtttcctg cgccgccagc
     2101 acaagggccg tgttaaagat attcagcgac gtattctcca tcgctcccat attaaaatcg
     2161 ttcacagcga caatgttgaa catatccagc tcatattcca gcccgtacgt atcctcatcc
     2221 catttcatgg acttctttaa ggactccatt gcatgcccgc attggtcctc atcacccggt
     2281 cgcacataga tataaagctc aacagcgcgg ccggacttcg tggtgtacgt gtcctgaata
     2341 tgcgtcaaat cccccgcgac cagcgcaaac aggtaacaag gcttggggaa agggtcgtgc
     2401 cacaccgcat aatgccgccc gccgtcagaa tcaccatgat caacccggtt gccgtttgac
     2461 aacaaaaccg ggtaatgtgc cttgctggct tcaacacgta cagtgaaaac cgttaaaaca
     2521 tcagggcggt ctggataata ggtaatcgtt cgaaaccctt cggcctcaca ttgtgtacaa
     2581 taagtatccc cggacttata cagcccctcc agccg
//
LOCUS       DAOSWR010000190         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_30992_length_2575_cov_5.080556, whole genome shotgun sequence.
ACCESSION   DAOSWR010000190 DAOSWR010000000
VERSION     DAOSWR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30992_length_2575_cov_5.080556"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK513_10390"
     CDS             complement(<1..792)
                     /locus_tag="PK513_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOO82896.1"
                     /translation="MAYKNFDDLSLSPTSGAANETLQINGPSVDLPDASYVKDAALER
                     DGADLVLDGPQGTLTIEGYFSAAETPDLVAPNGETLTPELVNSFARSPAQYAQSHSMN
                     DESPVGSVDEISGEATITHLDGSVEHITQGMPIYQGDVIETAADGAVNIAFIDETSFA
                     VSEDARLAIDEYVYDPSTESGSQDFSVLKGMFVFTSGLIGRDDPDDVNIDTPSGSIGI
                     RGTIIAGNVDTGEITVVEGAIVVRDLSGHEMTLATQFETAKLDPTG"
     gene            complement(1023..1808)
                     /locus_tag="PK513_10395"
     CDS             complement(1023..1808)
                     /locus_tag="PK513_10395"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOO82897.1"
                     /translation="MSNLKGKTAIITGSTSGIGLGIAQGLAAAGVNIVMNGFGDPDEI
                     ETHRTALEKQGIKAIYHAADMTRPNEIKDLISTARTELGSVDILVNNAGIQYVAPIED
                     FPPEKWDAIIAINMSSAFHTIHHALPLMRKQGWGRIINIASAHGLVASPYKSAYVTAK
                     HGILGLTKTVALECAENNITVNAICPGYVKTPLVEGQIADTAKARGISEEEVVNDVLL
                     KAQWTKKFVSVEQLSGLALFLCSDAAENMTGGHISVDGGWTAA"
     gene            complement(1982..2380)
                     /locus_tag="PK513_10400"
     CDS             complement(1982..2380)
                     /locus_tag="PK513_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOO82898.1"
                     /translation="MSIADELQILKFKPEERIISQGKHSDKAYMILSGKVRVFMEDGT
                     KIVELATLGEDEIFGESAIFSGEAYGANVEAVEESELYVITKDSLNEMLKSTDPIIRA
                     LIQMLIARLNATNEALLKSETREFIDVALI"
BASE COUNT          551 a          547 c          705 g          772 t
ORIGIN      
        1 gccagtcggg tcgagctttg ctgtttcgaa ctgtgtggcc agtgtcattt cgtgaccgga
       61 aagatcgcgc acaacaattg cgccttcaac aactgtgatt tcgccagtgt caacatttcc
      121 tgcaatgatg gttccgcgga tgccgatcga gccgctgggg gtgtcgatat tcacatcatc
      181 gggatcatcg cggccaatga ggccggaggt gaaaacaaac atacctttga ggacggaaaa
      241 gtcctgcgat ccggattctg ttgaaggatc ataaacatat tcgtcgatgg ccagacgtgc
      301 gtcttcagat acggcaaagc tggtttcatc aatgaaggct atattcactg cgccgtcggc
      361 tgcggtttcg ataacgtcgc cctggtatat gggcatgcct tgtgtgatgt gctcaacaga
      421 gccgtccaag tgtgtgattg tcgcctcgcc ggagatttca tcaacgctgc cgaccgggct
      481 ttcatcgttc atgctatggc tttgagcata ttgagcgggg ctgcgggcaa aggaatttac
      541 aagttcgggt gtcagggttt cgccattggg ggcaacgaga tcaggggttt ctgccgcaga
      601 gaaatatcct tcaattgtga gcgtgccttg tggaccgtca agaacgagat ctgcgccgtc
      661 gcgctcaagt gcggcgtctt tcacgtatga ggcgtcaggc aggtctacgg acgggccgtt
      721 gatctgcagc gtttcgtttg ctgcgccgga tgttggtgaa agtgaaagat cgtcgaaatt
      781 tttgtaagcc attgtcccta tcccttattt cttacgctaa gtgtacccca aaattgttca
      841 aaagtatttc ataaccctat aggccgcata tttgcagctc atcccttatt ttacggtaaa
      901 aagtcttaaa aagagctgat ttttacgtaa aattttatgt aattatatct acaaggttat
      961 atgttgtcgg tagcggtttg aaagctgttc ttctttgtaa cgtattgatt tattgtgata
     1021 ttttatgcag ctgtccagcc gccatcgacg gagatgtgtc cgcccgtcat gttttcggcg
     1081 gcgtcggagc ataaaaatag cgccaggccc gataattgtt caacggaaac gaatttttta
     1141 gtccattgcg ctttgagtaa aacatcgttg acgacttctt cttctgaaat gccgcgggct
     1201 ttggctgtgt cggcgatctg gccttcgacc agcggggttt tgacgtaacc ggggcagatg
     1261 gcgttgacgg tgatattatt ctcggcgcat tcaagggcga cggtttttgt gaggcctaat
     1321 atgccatgtt tggcggtgac ataggccgat ttataagggg aggcgacgag gccgtgggca
     1381 gaggctatgt tgatgatgcg gccccaacct tgtttgcgca tcaggggcag ggcatggtgg
     1441 atggtgtgga aagcgctgct catgttgatg gcgatgatgg cgtcccattt ttcggggggg
     1501 aagtcttcga tgggggcgac atattggatg cctgcgttat tgacaaggat gtcgacgctt
     1561 cccaattcag tgcgggcggt agaaatcaaa tctttaattt cattgggccg ggtcatgtcg
     1621 gcggcgtgat agattgcctt tatgccttgt ttttccagtg cggtgcggtg ggtttcgatt
     1681 tcatcgggat caccgaagcc gttcattacg atattgacgc cggcggcggc cagtccttgt
     1741 gctatcccta agccgatacc gctggtggat ccggtgatga tggcggtttt tcctttgaga
     1801 ttactcattc tttgtctccg cttcgatgaa aatgcgcttg attaaagatg ggccgttttg
     1861 atttctctgt ccagtgtttg ggtaatttat tctatgcgac cggtctttaa tccgttttta
     1921 aatttgagag agatgttggc gaggatagtt ttttagagcc tgaagccatg gagcgttgtt
     1981 tctatatcag ggcgacgtct atgaattccc gggtttcgct tttcagtagt gcttcgttag
     2041 tggcgttgag gcgtgcgatc agcatctgga tgagggcgcg tatgatagga tccgtgcttt
     2101 taagcatctc gttgagtgag tccttggtga tgacatagag ttcgctttct tcaacggctt
     2161 caacgtttgc gccgtaggct tcgccgctaa aaatagcgct ttcgccgaag atttcgtctt
     2221 cacccagcgt ggcgagttcg acgattttgg taccatcttc cataaaaacc cggacctttc
     2281 ctgacaaaat catataggct ttatcggagt gtttaccttg tgagataatc cgttcttcag
     2341 gcttaaattt aagaatttgt aattcgtctg caatactcat attttttccc tcaaccttaa
     2401 ttctaatatt ttttgggaaa tgaataaagc ataagagtgt tttttagggt gatttttatg
     2461 atgaaggggt atggacaaga gcggtccttt ttcctataac gctctatcgt ttgatctctc
     2521 aaacatgccc gggtagctca gtggtagagc aggggattga aaatccccgt gtcgg
//
LOCUS       DAOSWR010000191         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_31010_length_2574_cov_5.354903, whole genome shotgun sequence.
ACCESSION   DAOSWR010000191 DAOSWR010000000
VERSION     DAOSWR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31010_length_2574_cov_5.354903"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..893
                     /gene="rfbD"
                     /locus_tag="PK513_10405"
     CDS             87..893
                     /gene="rfbD"
                     /locus_tag="PK513_10405"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOO82899.1"
                     /translation="MTRKTCDLTDKNSVAHTVRNLYEEKNIRIFINAAAYTAVDKAED
                     EPAAALAVNWYGAGHLATEISKYEDAVLIYLSSDYVFDGKNRTPYEENAPANPLSVYG
                     LSKFAGEIVTRSFVSRHIILRTSWVFGADGQNFLKTMLALGKEKDELRIVNDQFGAPT
                     YSCDVAKALWHIAKTVQKPGFKEWGLYHYTGQGPVNWYDFAAHIFTQHKKNGGKVPHN
                     LIPVPSIAFPQKASRPSYSYMSCARIKKVFGLEQSDWKKGIRETLMLLFL"
     gene            complement(1014..1946)
                     /locus_tag="PK513_10410"
     CDS             complement(1014..1946)
                     /locus_tag="PK513_10410"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009605070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO82900.1"
                     /translation="MKLIAGNSNRPLAEAISSYLNVPLTQASLKRFSDMEVFVEIMEN
                     VRGEDCFFIQSTSFPANDHLMELLIAIDALKRGSARRITAVIPYFGYARQDRKTGSRT
                     PISAKLVANLITAAGADRVLTMELHAGQIQGFFDIPVDNLVAGPVFVPQILDEFDGEE
                     ICIVSPDVGGVVRARAMAKRLNASLAIIDKRREKAGVSEVMHVIGEVKGKVCILVDDI
                     VDSGGTLCNAAVALKENGAKSVYSYVVHGVLSGGAVSKIEASPMEKIVITNTIAATES
                     VSSSDSVEQLSVAPLIGEAMRRINEERSISALFK"
     gene            complement(2067..>2574)
                     /locus_tag="PK513_10415"
     CDS             complement(2067..>2574)
                     /locus_tag="PK513_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOO82901.1"
                     /translation="AMVTDVSGLALGVLTADCAPVLFYGKKSDGAPVVGAAHAGWGGA
                     LKGVLDNTLAMMLELGAVPESIRACIGPCIGQKSYEVRDDFADTFLEEDGRNEVFFTA
                     GRCAGHLQFDLPGYCAAKLGRAGVKNVLIKDLDTYFNEEDFFSYRRATHRHEKDYGRQ
                     ISVIMIQE"
BASE COUNT          755 a          734 c          474 g          611 t
ORIGIN      
        1 tcggtcatag atcgtagctt aactcactgt ccgaattctt gggaccacct ctcagatcca
       61 gtctctgagg atcataaggc gtaccgataa cacgaaaaac atgcgacctt actgataaaa
      121 attctgttgc ccataccgtc cgtaatctct acgaagaaaa gaacattcgt attttcatta
      181 atgccgcagc gtatacagca gtagacaaag ccgaggacga gccagcagca gcacttgctg
      241 ttaattggta tggagcaggg catttagcta cagaaatttc taaatatgag gatgcagttc
      301 ttatttattt atcgtcagat tatgttttcg atgggaagaa ccgaacacct tatgaagaaa
      361 acgcacccgc taatccactt tcagtatatg gcctatcaaa atttgctggt gaaattgtca
      421 cacgttcttt tgtttcacgc catattatct tgcgaacctc atgggttttt ggcgcagacg
      481 gacaaaactt tctcaaaacc atgcttgcat taggcaaaga aaaagatgaa ttacgaattg
      541 taaatgacca gtttggcgcg ccaacctaca gttgcgacgt tgcaaaagcc ctatggcaca
      601 ttgccaaaac cgttcaaaaa cccggcttta aagaatgggg gctttatcat tacacaggtc
      661 aaggacctgt gaattggtat gattttgccg ctcatatttt tacacaacat aaaaaaaacg
      721 gcgggaaagt accacacaat cttattcctg taccatccat agcgtttcca caaaaagcat
      781 caagaccatc ttattcctat atgagttgcg cacgcataaa aaaggttttt ggccttgagc
      841 aaagcgattg gaaaaaaggc ataagagaaa ccttgatgct actgttcctc taacaaattt
      901 ctattgacga tacagtcatt taaagtactg atccggcaca ctgggcgcat ggccaatggc
      961 aaagcctttg tccttcagtt ccccaaacat ttttgctctg ctgtaaaaat aacctactta
     1021 aacagtgctg aaatagagcg ttcctcatta atccggcgca ttgcctcacc aatcagcggc
     1081 gcgacagaga gctgctcaac gctgtcgctg gaactcacag actcggtcgc cgcaattgta
     1141 ttggtaatca caattttctc catcggagac gcttcaatct tgctcaccgc cccgcccgat
     1201 aataccccat gcacaacgta ggaatacaca gacttggccc cgttttcttt cagcgccacc
     1261 gccgcattac acaacgttcc gccggaatcg acaatatcgt caaccagaat gcagactttg
     1321 cctttaacct ccccgattac atgcataacc tcggaaaccc cggccttttc ccggcgttta
     1381 tcgataatcg ccaaagacgc attcaaccgc ttggccatcg cccgcgcccg cacaaccccg
     1441 ccaacatccg gcgaaacgat gcaaatttcc tcaccgtcaa attcatcgag aatttgtggc
     1501 acaaacaccg gcccggccac cagattatcc accggaatat caaagaaccc ttgaatttga
     1561 ccagcatgca gctccatggt caaaacccgg tccgcgcccg ccgccgtaat caaatttgcc
     1621 actagcttgg ccgaaatcgg cgtccggcta cccgtcttac gatcctgccg cgcatacccg
     1681 aaatacggaa tcaccgctgt aatccgccgc gccgacccgc gtttgagcgc atcaatcgca
     1741 atcaacagct ccatcaaatg gtcattggcc ggaaaagatg tcgattgaat aaaaaagcaa
     1801 tcctcgccgc gcacattctc cataatctca acgaaaactt ccatatcgga aaaacgcttc
     1861 aaactcgcct gggtcaacgg cacattcaaa taagaagaaa ttgcttcagc cagcggacga
     1921 ttggaattcc cggcaatcag tttcatttta accctaactt gttattttta ttaaaaaata
     1981 cgcatatcaa gctgcatacg agcgcactat acggaaaaat caccagcaaa atcaagcacg
     2041 cttttggata aaaaacccgg ttatgatcac tcttgaatca taataaccga aatctgccga
     2101 ccataatctt tttcatgccg atgcgtcgcc ctgcgataac tgaaaaaatc ctcttcatta
     2161 aaatacgtat ccagatcctt gatcagcacg tttttaacgc ccgcccggcc cagcttcgcc
     2221 gcgcaatagc ccggcaaatc aaactgcaaa tgccctgcgc accgacctgc cgtgaaaaac
     2281 acctcattcc taccatcttc ctctaaaaac gtatctgcaa aatcatccct cacctcatag
     2341 gatttctgcc caatgcacgg ccctatacag gcgcgaatac tttccggtac agcgccaagc
     2401 tccagcatca tagctaacgt attatccaaa acccctttca acgcaccacc ccagccagca
     2461 tgtgcagccc ccacaaccgg cgcaccatca cttttttttc cataaaataa cactggcgca
     2521 caatccgccg ttaaaactcc caacgccagg cctgacacat ccgtaaccat cgca
//
LOCUS       DAOSWR010000192         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_31762_length_2536_cov_4.262394, whole genome shotgun sequence.
ACCESSION   DAOSWR010000192 DAOSWR010000000
VERSION     DAOSWR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31762_length_2536_cov_4.262394"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..366
                     /locus_tag="PK513_10420"
     CDS             142..366
                     /locus_tag="PK513_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HOO82902.1"
                     /translation="MHHQHHKGFIAFILSWKGVALILALAGLSYYLLTQHLDHLAVAL
                     PYLILLACPLMHIFGHGHGGHGDHNKEDKE"
     gene            363..1700
                     /locus_tag="PK513_10425"
     CDS             363..1700
                     /locus_tag="PK513_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014997020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOO82903.1"
                     /translation="MSHNHDRSAHYKENSLTLIGAVSMGTGVMIGAGIFALTGQVAEL
                     AREWFPLAFLAAAVITGFSAYSYVKMSNAYPSAGGIAMFLEKAYGKTFMTAGCALLMY
                     FSMVINESLVARTFGTYTLQLFDGGKDSWLVPALGVGLLAFAFLVNILGNKFIQTFSF
                     FMAFLKVAGIALLAAGGLWASGLSFESISVKPEETTPGGFLGATALAILAYKGFTTIT
                     NSGGEIKDPHKNVGRAIIIAIAICVVLYLMVTLAVGGNLSINEIITAKDYSLAEASRP
                     AFGEYGLWVTVGFAIIATVSGVIASVFAVSRMLAMLTDMKLVPHSHFGMPGDIQKHTL
                     VYTIVLAMVLTVFFDLSRIASLGAIFYIIMDIAVHWGVLRYLRKEIKAKAFVLITAII
                     LDLIVLSAFLIVKAQTDMLVIYVSLAGMIFVFAGERLFLKHYSRSDEGHSHGA"
     gene            1690..>2536
                     /locus_tag="PK513_10430"
     CDS             1690..>2536
                     /locus_tag="PK513_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOO82904.1"
                     /translation="MEHKHHKHSHENHSCCHHEHGKKAKPVKIPAGANVIYTCPMHPE
                     IRQEGPGNCPICGMALEPETITGEEGENPELTDFRRRFWIGLILTLPVFALEMGAHLL
                     NFHHYISGTASNWIQLVLATPVVLWAGWPFFERGWQSLKSRNLNMFTLIAMGTGVAWV
                     YSVIATVAPGLFPDTFKGHEGAVAVYFEAAAVIVVLVLLGQLLELKAREQTGGAIRAL
                     LDLAPKTARRINENGEDEDVPLDQIKAGDLLRVRPGEKVPLDGVVTEGSSAVDESMVT
                     GESMPV"
BASE COUNT          577 a          574 c          697 g          688 t
ORIGIN      
        1 ttctggatgc acagcgcact ctgctagagt cagaagatca gtatgaacgg gcacgatacg
       61 aaacactggc cgcacgcgta gagttattta aggccatggg cggcggctgg gaagaagaat
      121 aagaaaaaag gagttagaat tatgcatcat caacatcata aaggttttat agctttcata
      181 ttgtcatgga agggcgttgc cctgattttg gctttagccg ggctgagtta ttaccttctg
      241 acgcagcatt tggatcatct ggctgtggcg ttaccgtatc ttattctgct ggcctgcccc
      301 ctcatgcaca tattcggcca tggtcacggc gggcatggag accataacaa ggaggataag
      361 gaatgagcca taaccacgac cgttcagcac actacaaaga aaacagcctg accttaatcg
      421 gcgcggtttc aatgggaacc ggggtgatga tcggggccgg gatattcgcc ctgaccgggc
      481 aggtggcgga gctggcgcgg gaatggttcc cgctggcttt tctggcggcg gcggtgatta
      541 cgggctttag cgcttactct tacgtgaaaa tgtccaacgc ctatccgtct gcgggcggca
      601 ttgccatgtt tttagagaaa gcctacggca agacttttat gacggccggg tgcgcgttgc
      661 tcatgtattt ttccatggtt attaacgaaa gccttgtcgc gcggacgttc ggtacctata
      721 cactgcaact ttttgacggc gggaaagata gctggctggt tccggctttg ggggttggac
      781 ttctggcctt tgcgtttctc gttaatattc tcggtaacaa attcatccag acattttcgt
      841 tcttcatggc cttcctgaaa gttgccggta ttgcgctatt agctgcaggc ggtttgtggg
      901 cctcgggttt aagttttgaa agcatttccg tgaaaccgga agaaaccacg cccggcgggt
      961 ttttaggggc aacggcgctg gccatactcg cttataaagg ctttaccacc atcaccaaca
     1021 gcggcggcga aattaaagac ccgcataaga atgtcgggcg ggcgattatc attgcgattg
     1081 ctatttgcgt tgttctttat ctcatggtca cgctggcggt tggtggtaat ctttcgatta
     1141 acgagattat tacagccaaa gattattctc tggccgaggc ttcacggcct gcctttggag
     1201 aatacggcct gtgggtgacg gtcggctttg ccattatcgc caccgtgtcc ggtgtgattg
     1261 ccagcgtgtt tgcggtgtcc cgaatgctgg ctatgctcac cgatatgaaa ctggttccac
     1321 atagtcattt cggtatgcca ggcgatattc aaaaacatac gctggtctat acgattgttc
     1381 tggccatggt gctgacggtc ttttttgacc tcagccggat tgcctcactc ggcgcgattt
     1441 tttacatcat catggatatt gctgtgcatt ggggcgtatt gcgttatctg cgcaaagaga
     1501 ttaaggccaa agcctttgtg cttatcacgg cgattattct ggatttgatc gtattgagcg
     1561 cattcctgat tgtaaaagca caaaccgata tgctggtgat ttatgtttcg ctggcgggca
     1621 tgatctttgt ttttgccgga gagagactat ttttaaaaca ctattccaga tcagatgaag
     1681 gacactcaca tggagcataa acaccataaa catagtcacg aaaaccattc atgttgtcat
     1741 catgagcatg ggaaaaaagc aaagcctgtt aaaattcctg ccggtgccaa tgtgatttac
     1801 acctgtccta tgcacccgga aatccggcag gaagggccgg ggaactgtcc aatatgcggc
     1861 atggcgctgg agccggaaac gatcaccggc gaagaaggcg aaaacccgga actgactgat
     1921 tttcgccgcc gtttctggat tggtcttatc ctaacattgc cggtttttgc gttggaaatg
     1981 ggcgcacatc ttttaaattt tcatcattat atcagcggta ctgcttcaaa ctggatacaa
     2041 cttgttttgg caacgcctgt agttttatgg gcaggatggc cgttttttga acgcggctgg
     2101 caatccctga aaagccgcaa tctgaatatg tttactctta tagcgatggg aacaggtgta
     2161 gcgtgggttt acagcgttat tgctacggta gcgcccggct tatttcccga tacgttcaaa
     2221 ggacatgaag gtgctgttgc cgtttatttt gaagcggcag cggtaattgt cgtgcttgtt
     2281 cttctcggtc agctcttgga attaaaggcg cgcgaacaga ctggtggggc tatccgtgcg
     2341 ctgctcgatc tcgcgccgaa aaccgcacgc cggattaatg aaaatggcga ggatgaagat
     2401 gtgccgcttg atcaaataaa ggcgggcgat ttgctccgcg tacgtcccgg tgagaaagtg
     2461 ccgctggatg gtgttgtgac cgagggcagc agtgcggtcg atgaatctat ggtcactggg
     2521 gaatccatgc ccgtta
//
LOCUS       DAOSWR010000193         2481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_32986_length_2481_cov_4.043281, whole genome shotgun sequence.
ACCESSION   DAOSWR010000193 DAOSWR010000000
VERSION     DAOSWR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32986_length_2481_cov_4.043281"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PK513_10435"
     CDS             <1..1084
                     /locus_tag="PK513_10435"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOO82905.1"
                     /translation="EVMGLALPGANGIPAEDHTKESYAKACGEAVVECLKNGILPRDI
                     VMRKSLENAAAIVAATGGSTNAGLHLPAIAHEAGIDFPLEEVAEIFKKTPLIANLRPG
                     GKYVAKDLYDIGGVGVVIKELLDNGFLHGDCLTITGKTLAENYADVRFPEGQDVVFPC
                     SNPIHPTGGVVGLTGNLAPEGAIVKIAGLEEFVFEGPARIFDGEEAAFRSVQAREYED
                     GDVIVVRYEGPKGGPGMREMLSTTAALYGQGAGSKVALITDGRFSGGTRGFCIGHVGP
                     EAAVGGPIGLIQDGDIIRIDAEAGTLEVLLSDEELAARRSEWKPRKTNYNSGALWKYA
                     DQVGPARDGAVTHPGAKEETHIYVEL"
     gene            1166..>2481
                     /locus_tag="PK513_10440"
     CDS             1166..>2481
                     /locus_tag="PK513_10440"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOO82906.1"
                     /translation="MTRKFLLYSGLVVGAAIFAVPIVPVSAQEPVSAEQLLNVQAGVS
                     GAIYATFEDRAACKAFKDVKALEVFYSQRGYEPYWVSRSRAKTNARDFLEIIEKSWRH
                     GFNPYSYHLKRLHELVGQRDEALLGELDVLLSDAYLRLAQDLTGIRVNPGSLKSHWRY
                     WRAPLAGAYLFERLNAQRDIEDLIESFEPQGQTYKQIQRELVTLVNSKPEDYEKVLPI
                     RMKGLLKPNERDESVPDLRLRLNAGRPQTDDPYLYDDRLAAAVIRFQRDNSLKDDGIV
                     GQQTLDILNRAREHKILQLVANLERLRWVEEEKPDTFVVVNVPSAMLWAVEGGHVAFE
                     MPVIVGRKERPTNIFRAEIHGVRLNPDWTVPPTIKRDDILPKLREDPEYLTHKGMQLI
                     SGRGEGAMTLDPLVFDWENVTEEELKDLRMVQIPGAHNPLGSIRVL"
BASE COUNT          539 a          456 c          823 g          663 t
ORIGIN      
        1 ggaagtgatg gggcttgctt tgccgggtgc taacggcatt ccggcagagg atcatacgaa
       61 agaaagctat gccaaggctt gcggtgaagc tgtggtggag tgtcttaaaa atgggatctt
      121 gccgcgtgat attgtgatgc ggaaatcttt ggaaaatgcc gcggcgattg tggcggcaac
      181 gggcggcagc accaatgcgg ggctgcattt gccggcaatt gcgcatgagg cgggaattga
      241 ttttccgctg gaagaggtgg ctgaaatctt caagaaaaca ccattgattg ccaatttgcg
      301 ccccggcgga aaatatgtgg ccaaggatct gtatgatatt ggcggtgttg gcgtggtgat
      361 taaggaattg ctggataatg gctttttgca tggtgattgt ctgacgatta cgggtaaaac
      421 gctggctgaa aattatgctg atgtgcgctt tcctgaaggt caggatgtag tgtttccatg
      481 ctctaacccc attcatccga ctggcggcgt tgtcggtttg acaggcaatt tggcaccgga
      541 gggggctatt gtaaaaattg ccgggctgga ggagtttgtt tttgaagggc cggcgcggat
      601 ttttgatggt gaagaagcgg cgttcaggtc ggtgcaggcg cgtgagtatg aagacggtga
      661 tgtgattgtt gtgcgctatg agggtcccaa aggcggtccg gggatgcgtg agatgctctc
      721 gacgacggcg gctttatacg ggcaaggggc cggaagtaag gttgccttga tcactgatgg
      781 gcgtttttcc ggcgggacgc gcggtttctg tatcggtcat gttgggccgg aagctgccgt
      841 gggcggtccg atcgggctta ttcaagatgg tgatattatt cgtattgacg cggaggctgg
      901 tacgctggag gttctgttat ctgatgaaga gctggcggcg cgccgcagcg aatggaagcc
      961 gcgtaagacg aattataatt ccggcgcttt gtggaaatat gccgatcagg ttggcccggc
     1021 gcgtgatggt gcggtaacgc atccgggggc gaaggaagag actcatatat atgtggagct
     1081 ttaggtgcgc gcttagttcg tgaaaaatta ttgttttcgt gttacgttgc ccgggagtga
     1141 ttcacacttt cctttgatgt tgtttatgac gcgtaaattt cttttgtatt cgggtttggt
     1201 tgtgggcgct gcaatatttg cggtgcctat tgttcctgtg agcgcacaag aaccggtgag
     1261 cgccgagcag ttgctcaatg tgcaggcagg tgtcagtggt gcgatctacg ctacatttga
     1321 agaccgtgcg gcatgcaagg catttaaaga cgtgaaagcg ctggaggtat tttatagcca
     1381 gcgtgggtat gagccgtatt gggtttcgcg ctctcgggca aaaacgaatg cgcgggattt
     1441 tctggagatt attgaaaaga gctggcggca tggctttaat ccttatagct atcatttgaa
     1501 acggctgcat gaattggtgg gacagcgcga tgaggcactt ttgggtgagc ttgatgtgtt
     1561 gctcagtgat gcttatttac gcttggcgca agatttgacg ggcattcggg tgaatccggg
     1621 ttcattaaag tcgcattggc gttattggcg tgcgccgctt gccggggcgt atttatttga
     1681 gcgattgaat gcgcaacgcg atattgagga tttgatcgaa tcttttgaac cgcaggggca
     1741 gacttataag cagatacagc gggaattggt tacactggtg aatagtaagc cggaagatta
     1801 tgagaaagta ttgccgattc gtatgaaggg attattaaag ccgaatgagc gggatgaatc
     1861 tgtgccagat ttgcgtttgc gactgaatgc gggtaggccg cagacggatg atccgtattt
     1921 gtacgatgat agacttgctg cggcggttat tcggtttcag cgggataata gcctgaaaga
     1981 tgatggaatt gtcgggcagc aaactctgga tattcttaac cgcgcgcgcg agcataaaat
     2041 cttgcagctt gtcgctaatc ttgagaggct tcgctgggtt gaagaggaaa agcccgatac
     2101 cttcgtggtg gtgaatgtgc cttcggcgat gctctgggcc gtggagggcg ggcatgtggc
     2161 gtttgagatg cccgtgattg tggggcgtaa ggagcgtccg acgaatattt ttcgtgcgga
     2221 aattcatggt gtgcgactta atccggattg gactgtgccg ccgacaatta aacgtgatga
     2281 tattttacca aagttgcggg aagatcctga atatctgacg cataagggga tgcagcttat
     2341 cagtgggcgc ggggaagggg cgatgacgct tgatccgttg gtgtttgatt gggaaaatgt
     2401 tacggaagag gagttgaaag atctgcggat ggtgcagata cctggcgcgc ataatccgct
     2461 ggggagtatc cgggttttga t
//
LOCUS       DAOSWR010000194         2353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_35931_length_2353_cov_4.470409, whole genome shotgun sequence.
ACCESSION   DAOSWR010000194 DAOSWR010000000
VERSION     DAOSWR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35931_length_2353_cov_4.470409"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="purM"
                     /locus_tag="PK513_10445"
     CDS             <1..789
                     /gene="purM"
                     /locus_tag="PK513_10445"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOO82907.1"
                     /translation="QGAEPLFFLDYFATGKLDTGVAEDVIKGVAKGCELSGCALIGGE
                     TAEMPGLYADGDYDLAGFTVGAVEREDIITGADIGDGDTLLGLASSGLHSNGFSLVRK
                     IIESTQGFDYEASAPFAKGQTMAEALLTPTRLYVKSILAALTIRDDVGKPAIKGMAHI
                     TGGGLLENVPRMMPKGLCAEIDCTAWELPSVFKWLMEAGNLNANDMGTTLNAGIGMVV
                     AVPKAYKDAVRTSFEENGERVYEIGRVTKGGAPIVLRNAEECWG"
     gene            791..1390
                     /gene="purN"
                     /locus_tag="PK513_10450"
     CDS             791..1390
                     /gene="purN"
                     /locus_tag="PK513_10450"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOO82908.1"
                     /translation="MSADKLNLAVFISGNGSNLQALIDACKDPDFPARIALVVSNRPG
                     AYGLERAKKAEIPAITIDHKNYTSRSEFENALQDTLKDYPVDLICLAGFMRILSAGFV
                     NRWTNKIINTHPALLPKFGGKGMYGEHVHRAVLDAGEPQSGCSIHYVIPEVDQGPVIV
                     QKAVPVQAGDSVETLAARVIAQEHIAYPEAVRMIAEKQL"
     gene            1469..1624
                     /locus_tag="PK513_10455"
     CDS             1469..1624
                     /locus_tag="PK513_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82909.1"
                     /translation="MNHNSPIEDDPQQLQHANDLWRSFLHKSKISILLIVLVIIGLAL
                     AFVPASS"
     gene            1720..>2353
                     /locus_tag="PK513_10460"
     CDS             1720..>2353
                     /locus_tag="PK513_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HOO82910.1"
                     /translation="MDMIIVQAASKLKLTAAKALICFVLFALAFGVPVYCSFNDAQAQ
                     ITPSSRVELINDHERVFIGKNIYVTPDPERKFNFETLAKRHINNLKGTRQTSNVVHMG
                     AAPQPMWMIFSVTNKSRYDDWVLHFGDLFDGRFGLIRSIALYNASSGETIIKSPTADA
                     EAVHFERLQSNALALDIPAGKTQLFTLYLEAAPSVVNSIAPSIMSTKSYQM"
BASE COUNT          572 a          503 c          660 g          618 t
ORIGIN      
        1 cagggcgcgg agccgctgtt tttcctcgat tattttgcaa caggcaagct tgataccggc
       61 gttgcggaag atgtgattaa aggcgttgcc aagggctgtg agctttcagg ctgcgcgttg
      121 attggcgggg agacagccga gatgccgggt ctgtatgcag acggggatta cgatctggcg
      181 ggttttacag tgggtgcggt tgagcgtgaa gatataatca ccggcgcgga tattggcgat
      241 ggtgacactc tcttggggtt ggcatcttcg gggctgcatt cgaacgggtt ttcgctggtg
      301 cgtaagatta tcgaaagtac acaagggttt gattatgagg cgtctgcgcc gtttgcaaag
      361 ggccaaacca tggccgaagc cttgctcacg ccgacgcggc tgtatgtgaa atcgattttg
      421 gcggcgctca caatccgtga tgatgtgggc aaacctgcga ttaaggggat ggcgcatatt
      481 acaggcggag ggctgctgga aaatgtgccg cggatgatgc cgaagggctt gtgtgcggaa
      541 attgattgta cagcgtggga attgccgtct gtgttcaagt ggttaatgga ggcgggcaat
      601 ctgaatgcta atgatatggg cacgaccctg aatgccggca taggcatggt tgtggccgtc
      661 ccaaaggcct ataaagacgc tgtgcgcaca agttttgagg agaacggcga gcgtgtttat
      721 gagattgggc gggttacgaa aggcggtgcg cctatagtct tgcggaatgc tgaggagtgc
      781 tggggctagg atgagcgcag ataaactcaa tctcgccgtt tttatttccg gtaatggcag
      841 caatctgcaa gccctgatcg atgcgtgcaa ggaccctgac tttccggccc gcattgcgct
      901 agtggtttcg aaccggcccg gagcgtatgg tttggagcgc gcgaaaaagg ctgaaatccc
      961 tgctattaca atcgatcata aaaactatac gagccgtagc gaatttgaaa atgcgctcca
     1021 ggatacgctc aaagattatc cggtggattt gatctgtctg gccgggttta tgcgtatttt
     1081 aagcgcgggt tttgtcaatc gctggacgaa caaaatcatc aatacgcacc cggccctgct
     1141 gccgaaattc ggcggtaagg ggatgtatgg cgaacatgtt catagagctg ttctggacgc
     1201 tggcgagccg cagagcggtt gcagtattca ctatgtcatt cctgaggttg atcagggtcc
     1261 ggtgattgta caaaaggccg tgccggttca ggcaggcgat agcgtggaaa cgctggcggc
     1321 gcgcgtgatt gcgcaggagc atatcgcata ccccgaagct gtgaggatga ttgctgaaaa
     1381 gcaactttaa aaaaccatac ttgaaatcga tttcattttc ccgtctaatt ttcaggaaag
     1441 agactacaag ccttaaaaga ggatcagtat gaatcataat tcccccatag aagacgaccc
     1501 ccagcaattg cagcatgcta acgatttatg gcgcagtttc ttacacaaaa gcaaaatcag
     1561 cattttactg attgttctgg ttatcattgg attggctctg gcgttcgtgc ctgcaagctc
     1621 ttagaagtaa aatattccgc atttttatca aaagcttaac cttttccagc gtaaattgac
     1681 gtatagtatc tggtgggtgg ggttttaaaa aaatttcgga tggatatgat cattgttcag
     1741 gctgcatcaa aactaaaact aactgccgct aaggcgttga tatgttttgt gctttttgcg
     1801 ctggcattcg gtgtgccagt ttactgctct ttcaacgatg cgcaggcgca aattacccct
     1861 tcctcacggg tagagctgat taatgatcat gagcgcgttt tcattggcaa aaacatatat
     1921 gtgacgcctg atcctgaaag aaaattcaac tttgaaactt tggctaaacg ccatatcaac
     1981 aacctgaaag gtacgcgcca gacaagtaat gtcgtacata tgggtgcggc gccacagcct
     2041 atgtggatga ttttttctgt gactaataaa tcacgatatg atgattgggt tttacatttt
     2101 ggggatttgt ttgacgggcg ttttgggctg atccgctcca ttgcgcttta taatgccagc
     2161 agcggcgaga cgattataaa atcaccaaca gctgatgctg aggccgtgca ttttgagcgg
     2221 ttgcagtcca atgctctggc gctggacatt cctgccggta aaacccagct tttcactttg
     2281 tatctggaag ccgcccctag cgttgtgaac agcattgcgc cttctattat gagcacaaaa
     2341 tcctatcaaa tgc
//
LOCUS       DAOSWR010000195         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_38149_length_2266_cov_4.165084, whole genome shotgun sequence.
ACCESSION   DAOSWR010000195 DAOSWR010000000
VERSION     DAOSWR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38149_length_2266_cov_4.165084"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /gene="asnB"
                     /locus_tag="PK513_10465"
     CDS             complement(<1..1617)
                     /gene="asnB"
                     /locus_tag="PK513_10465"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO82911.1"
                     /translation="MCGITGFYTFKADRPRTELHVIGKNMADALSHRGPDAGGLWQDP
                     DLPLVLGHRRLSIIDLSEDGAQPMISASERYVTVFNGEIYNFRTLKKDLEKHGHTFKG
                     RSDTEVILAAIEAWGLSLALQKLSGMFAFALWDRKDRTLSFARDRLGKKPLYIGWAGA
                     NLIFGSQLEALRTHPDFIPALNPEALQAFLTLGYVEAPLSIYEQIWQLPPGHRLTLDL
                     TALRPGENLPAQMKPYWQASSALEQAKTRPNPLNENALIDEFESLLSQCVSDRMISDV
                     PLGAFLSGGIDSSSIVALMQKHASQPVKTYAIGFEDAGYNEAPFAAKIAAHLGTDHHE
                     HFCTQKDALDLIPRLPEIYDEPFADISAIPTLLVSRFARESVSVALSGDGGDEMLGGY
                     SRHVQGPQIWKNMHKMPRILRIGLSNIITRMPVNSLDQLNRKHPQLGVKLHKVADILS
                     LSSQEDIYRRLIGKGAQGYYQKELSLPGVELSFAEKMMFWDTTGYLPHDILTKVDRAS
                     MAYGLEARAPLLDTRIFDYVWALPENVKIRG"
     gene            1692..>2266
                     /locus_tag="PK513_10470"
     CDS             1692..>2266
                     /locus_tag="PK513_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82912.1"
                     /translation="MNIDLLFTAAGEAGLISSENLVKKVAGVVFDIATGTLTFEYVDM
                     DFLELNIPVEKEFNAALDVCPQIHIGAIKDGNIAQAYQVPFMFLDDPYRGEAFQGLAP
                     PSQPLEAFDYFVKSTVFGQPVHREDLGDESAMGCVLGDAVPSALQFAPHLARRHQMEI
                     APKSAPNAPGPTMPGLGAGGGHASGSAIGRIT"
BASE COUNT          493 a          552 c          664 g          557 t
ORIGIN      
        1 gccacggatt ttgacatttt ccggcaaggc ccagacatag tcaaatatcc gcgtatcgag
       61 caatggcgca cgggcttcca agccataggc catggatgcc cggtcaactt tggtgaggat
      121 gtcgtgtggc aaatagccgg tcgtgtccca gaacatcatt ttttcggcaa aggagagttc
      181 gacgccaggc aggctgagtt ctttttggta gtacccttgc gcgcctttac caatcaggcg
      241 gcgatagatg tcttcttgtg agctcagcga gagaatgtct gcgactttgt ggagtttaac
      301 gcctaattgc gggtgtttgc ggtttagctg gtccagtgaa ttgaccggca tgcgagtgat
      361 tatattggat aagccgatgc gcaaaatacg gggcattttg tgcatgtttt tccagatttg
      421 gggcccctga acatggcggc tataaccacc cagcatttca tcgccgccgt caccggaaag
      481 cgctacgctc acgctttcgc gggcaaagcg gctgaccagc aaggtgggaa tagctgaaat
      541 atcggcgaag ggttcgtcgt aaatttccgg caagcgcggg atgagatcca gcgcatcctt
      601 ttgtgtgcag aaatgttcgt ggtgatcggt gcccagatgc gcggcgattt ttgcggcaaa
      661 aggtgcttcg ttatagcctg catcttcgaa accgatggca taggtcttca cgggctggct
      721 cgcatgtttt tgcatcagcg caacaattga tgagctgtcg atgccgcccg agagaaacgc
      781 gccgagtgga acatcagaga tcatacgatc gctgacgcac tggctgagca gcgattcgaa
      841 ttcgtcgatg agcgcatttt cgtttaaggg attaggccgg gttttggctt gctccaacgc
      901 gctagaggcc tgccaatagg gtttcatctg cgcgggcaga ttttctccgg gcctgagcgc
      961 tgttaaatca agcgtaagac ggtggccggg tggaagctgc cagatttgct cataaattga
     1021 caacggcgct tcgacataac cgagcgtcag aaatgcttgc agcgcttcgg gattcagggc
     1081 cgggatgaaa tccggatgcg tgcgcagggc ttcgagttgc gagccgaaga tgagattggc
     1141 cccggcccag ccgatataga gtggtttttt ccccagccgg tcgcgggcaa agctgagcgt
     1201 gcggtctttg cgatcccaca aggcgaaggc aaacatgccg ctcagttttt gcagcgcgag
     1261 gctgagaccc catgcttcaa tggcggcgag aatgacttca gtatcgctgc ggcccttaaa
     1321 agtatggcca tgcttttcga gatccttttt cagggtacgg aaattataaa tttcgccgtt
     1381 gaatactgtg acgtagcgct cactcgcgga gatcatcggc tgcgcgccgt cttcgctcag
     1441 atctatgatt gacaggcggc ggtgacctaa caccagcggc aagtccgggt cttgccagag
     1501 cccgcctgcg tcggggccgc gatggctcaa cgcatcggcc atatttttgc cgatgacgtg
     1561 caactcggtg cgaggccggt ctgctttaaa tgtataaaaa cctgttatac cacacataaa
     1621 ttcactgttt ctcgtcaggc tgaatatctg ataaaatgaa ttatactata cgcagagggt
     1681 taaacaatct catgaatatt gatcttcttt ttacggccgc gggagaagcg ggtctgattt
     1741 ccagtgaaaa cctggtgaag aaggttgctg gcgttgtgtt cgacattgct acagggactt
     1801 tgacgtttga atatgtcgat atggattttc tggaattgaa tatcccggtg gaaaaggaat
     1861 ttaacgcagc gttggatgtg tgcccgcaaa ttcatatcgg cgcaattaaa gacggtaata
     1921 ttgcccaggc ttatcaggtg ccgtttatgt ttctggatga tccgtatcgt ggtgaggcct
     1981 ttcaaggcct cgccccgccg tctcagccgc tggaagcttt tgactatttc gtgaaatcca
     2041 ccgtgtttgg gcagcccgtt caccgcgagg atttgggcga tgaaagcgcg atgggctgcg
     2101 ttctaggcga tgcggtgccc agcgctttgc aatttgcgcc gcatctggca cggcggcatc
     2161 agatggaaat cgcgccaaaa tctgcgccga atgcaccggg accgacgatg cccggtttgg
     2221 gcgcaggtgg gggccatgcc agcggtagtg ccattggccg catcac
//
LOCUS       DAOSWR010000196         2144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_41653_length_2144_cov_5.839636, whole genome shotgun sequence.
ACCESSION   DAOSWR010000196 DAOSWR010000000
VERSION     DAOSWR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41653_length_2144_cov_5.839636"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..340)
                     /locus_tag="PK513_10475"
     CDS             complement(110..340)
                     /locus_tag="PK513_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82913.1"
                     /translation="MYAKIRDLLTSAEQTFNIIEEYAALSPKRKVLVCDHFFIEGHEI
                     YENTVRLLWPEATKADMKKLSNFLILLKSTSH"
     gene            514..1257
                     /locus_tag="PK513_10480"
     CDS             514..1257
                     /locus_tag="PK513_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO82914.1"
                     /translation="MVYNSPAIILTNPQMGENIGAAARAMLNFGLTDLRLVNPRDGWP
                     NQRAIDMSSGALERMPPVGVFDTLGESISDLQLAYATTARRRDMIKPVLTPRTAAKDA
                     RGRLQNRQKIGFVFGAERTGLTNDEVALCSYIIQVPTNPEFSSLNLGQAVLLMAHELF
                     QAADDTSGRVMDTGDSPPALQEDCENFFKRLERELEAGGFFTAKDLKPTMVRNIRNIF
                     TRSELSMQEINTLQGIVSVLIGKKKAAEK"
     gene            complement(1291..1908)
                     /gene="rpsD"
                     /locus_tag="PK513_10485"
     CDS             complement(1291..1908)
                     /gene="rpsD"
                     /locus_tag="PK513_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO82915.1"
                     /translation="MSGKRISAKHKIDRRMACNLWGRTKSPYNLRQKGPGMHGERRGK
                     LSDYGQQLMAKQKLKGYYGNIGEKQFRSYYKEADRMRGDTGQNLIGLLECRLDAVVYR
                     ASFVPTVFAARQFVNHKHVTVNGKIVNIPSYQVKPGDVIEVREKGKAIPMVISATQQP
                     EREVPEYVDVDLKAMKATFLRIPALEDVPYPVQMEPNLIVEFYSR"
BASE COUNT          541 a          497 c          514 g          592 t
ORIGIN      
        1 acctgccttg tggggatagg ggaagcggca ggcggctttg gtttcacccg ttgatgtgga
       61 cccttaaaaa aacccctttt aaaccttagt tttgggtgaa accttattct taatgcgatg
      121 tacttttgag caaaatcaaa aaattactga gctttttcat gtcggccttt gtggcctcgg
      181 gccagagcaa acgcactgtg ttttcatata tttcatggcc ttcgataaag aaatgatcac
      241 aaaccagaac tttccgttta gggctaagcg cagcatattc ttcgataatg ttaaaagtct
      301 gctctgcgct tgttagcaaa tctctaattt tggcgtacat cctctttact cctttacgct
      361 catgcattgg tggcatagcg ttttaaatcg caattcttaa tgaagtgtta acaaaagcta
      421 acaaatttat gtaaatctgt aaaatttaat ttgattctct tttgtttttc aatattttaa
      481 gctcataaac gcttattgga actggtgtaa aaagtggtct ataacagccc ggcaatcatc
      541 ttaacaaacc cgcaaatggg ggaaaatatc ggcgcagcgg cgcgggcgat gctgaatttc
      601 ggtctcaccg atttacggct tgtgaaccct cgagatggct ggccgaacca acgtgcgatt
      661 gatatgtcct caggcgcgct ggagcggatg ccgcccgtgg gcgtctttga tacgctgggc
      721 gagtccattt ccgaccttca actggcttat gctacaacgg cgcggcggcg cgatatgatc
      781 aaacctgtgc ttacaccgcg gacagccgcc aaagatgcgc gggggcgctt gcagaaccgt
      841 cagaaaattg gctttgtgtt tggggccgag cgcacagggc tgaccaatga tgaggttgca
      901 ctatgctcat acattattca ggtgccgacc aatccggagt tttcttctct caatctcggg
      961 caagcggttt tgttgatggc gcatgagctg tttcaggctg ctgatgatac atccgggcgc
     1021 gtgatggaca cgggagacag cccccctgct ctgcaagaag attgtgaaaa cttttttaaa
     1081 cggctggagc gtgaactgga agctggcggc ttctttacag cgaaagacct caagccaacg
     1141 atggttcgta atatccgtaa tattttcacc cgttcggagc tcagtatgca ggaaatcaat
     1201 acgctgcaag ggattgtttc ggtgttgatt ggcaagaaaa aagccgctga aaaataagcg
     1261 gctctttgaa ttctcggttt tgaaacctga ttatcgtgaa taaaattcga cgatcaggtt
     1321 cggttccatc tgcaccggat atggcacgtc ttccaaagcc ggaatgcgca ggaacgttgc
     1381 tttcatcgct ttcaggtcaa catccacata ttcaggtact tcacgctcag gctgctgggt
     1441 tgcagaaata accataggaa tagcttttcc cttctcgcgc acttcaatca catcgccagg
     1501 cttcacttgg taagacggaa tgttcacaat tttaccattc acggttacgt gcttatggtt
     1561 tacaaactga cgcgccgcaa aaaccgtagg cacaaaagac gcacggtaaa caaccgcatc
     1621 gaggcggcac tccagcaagc caatcaggtt ttgacctgtg tcgccgcgca tacggtcagc
     1681 ttctttatag taactgcgaa attgtttctc gccaatattg ccataatagc ctttaagctt
     1741 ttgcttggcc attaactgct gaccgtagtc ggagagcttg ccgcggcgtt cgccgtgcat
     1801 gcccgggcct ttttggcgaa ggttataagg agatttggtc cgcccccaca ggttacaagc
     1861 catacggcgg tcaattttat gttttgcgga tatgcgctta ccactcataa ttaaggtcct
     1921 ttcatagaca aatcatttaa gttcatgtcc cgattcccct tttaggattg atacaatgat
     1981 caacgcgggc atatgtgccc gcttctcagg aatatgggga tttatagaga tttttggaag
     2041 aatgtcaaag aaaatgtcac aaacacaaaa ggttacagat atttatgtta tagataccca
     2101 cgggtaaacc cgtggttcta tggggctgcg ccgcgctttg cgca
//
LOCUS       DAOSWR010000197         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Alphaproteobacteria bacterium isolate AMR_MDS_2132
            NODE_52764_length_1860_cov_4.945152, whole genome shotgun sequence.
ACCESSION   DAOSWR010000197 DAOSWR010000000
VERSION     DAOSWR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864199
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Alphaproteobacteria bacterium (wastewater metagenome)
  ORGANISM  Alphaproteobacteria bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:20:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,099
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 2 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Alphaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52764_length_1860_cov_4.945152"
                     /isolate="AMR_MDS_2132"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1913988"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PK513_10490"
     CDS             complement(<1..1014)
                     /locus_tag="PK513_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOO82916.1"
                     /translation="MSSLLLITVACGILALIYSALASRQIMAQSAGNARMQEIAGAIQ
                     EGAAAYLARQYRAIAVVGTAVAGLLYLLLGWHVAIGFIIGAFLSGLAGYNGMLVSVKA
                     NVRTTQAATESLGKALNVAFKAGAVTGMLVVGLGLLGVTGYYLYLRYSLGLSGLEGEA
                     AMSATRHVLEGLVALGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRN
                     PAVIADNVGDNVGDCAGMAADLFETYAVTVVATMLIAFSTFAPGAVEHMMVLPLFIGA
                     ICILGSVAGTFFVRLNEQKEGNDTSIMRALYKGFAASAIFSALLIAVALANIGVDMAV
                     PLVD"
     gene            complement(1282..>1860)
                     /locus_tag="PK513_10495"
     CDS             complement(1282..>1860)
                     /locus_tag="PK513_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO82917.1"
                     /translation="FFILVVAATIWLIVKRAGSLTVFYPERAIPRPWDLLPLALFALG
                     ALIVLGGGAAGAYFYFFQSAEASAVDTIDDHKDDKKKDDHSGHYEFVELDPLILPIVD
                     NNGVSQTVNLVVTLEVVDAKARAKVEMITPRLKDAFIQDMYGVLNKHAALKGGVIQVD
                     ILKKRLNAVSARVVGEDTVHDVLLQVVQQRPI"
BASE COUNT          571 a          586 c          337 g          366 t
ORIGIN      
        1 atcgaccaaa ggcacagcca tatcaacacc gatattcgcc aatgccacag caatcaacaa
       61 cgccgaaaaa attgcactcg ccgcaaagcc cttgtaaagc gcccgcataa tgcttgtatc
      121 attaccttct ttttgctcat taagacgcac aaaaaacgtc ccggccacag aacctaaaat
      181 acagatcgca ccaataaaga gtggcaaaac catcatatgc tcaaccgccc ccggagcaaa
      241 cgtgctaaac gcaatcaaca tcgttgccac cacggtcacg gcataggtct caaacaaatc
      301 cgccgccatt ccggcacagt caccgacatt atcaccgaca ttatcggcaa ttaccgccgg
      361 attacgtgga tcatcctcag gaatgcccgc ctcaactttt cccaccagat ccgcgccgac
      421 atccgcgccc ttggtaaaga ttcctccacc caaacgcgca aaaatcgaaa ttaacgacgc
      481 cccaaagcca agagccacca gcccttccag aacatgacgc gtcgcgctca tcgcggcctc
      541 accttcaagg ccgctcaaac ccagactgta acgcaaatac agataatatc ctgtcacacc
      601 aagcagaccc aacccgacaa ccagcatccc ggtcacggcg ccagccttaa acgccacatt
      661 cagggccttg ccaagacttt ccgttgccgc ttgcgtggta cgcacattcg ccttaacaga
      721 aacaagcatc ccgttataac cggccagccc cgataaaaat gcaccgataa taaaaccaat
      781 cgccacatgc caaccaagca gcaaatacaa aagcccggcc accgccgtgc caacaaccgc
      841 aatcgctcta tattgccgcg ccagatacgc cgccgcacct tcctgaatcg cccctgcgat
      901 ctcttgcata cgcgcatttc ccgcgctctg agccataatt tgccgtgacg ccaaagcgct
      961 gtaaatcaac gccaaaatcc cacaagctac cgtaatcaat aacagtgaag acatatcccc
     1021 tccctcactt gttgtttgta ataatcacac ataaacacac ccaagaacct actgaatttg
     1081 ttcaaggatt actcgaaatt gtacccatcc caaacatcaa ggcaagacga ttttacaaaa
     1141 cacacttaaa actcgcgaaa aaacagggga caaaaaaaac cgcccaaatg gcggtaaaag
     1201 tttttttgat gtatagaggg ttcacatcaa ggtttaaaat cgcgatagaa ttccaccctg
     1261 taaacggaag gatctaaatt cttatatggg ccgctgttgt acaacttgaa gcaacacatc
     1321 atgcaccgta tcttctccga ccactcttgc gctcacagca ttaaggcgct tcttcagaat
     1381 atcgacctga ataacgccgc ccttgagcgc cgcatgctta ttgagaacac catacatatc
     1441 ctgaatgaaa gcatccttaa gccgcggcgt aatcatctca accttagccc tggctttcgc
     1501 atccacgact tcaagcgtaa ccaccaaatt cacagtctgg cttacgccat tattatcaac
     1561 aatcggaaga attaaaggat caagctccac aaactcataa tgtccgctat gatcatcttt
     1621 tttcttatca tctttgtgat catcaatcgt atcgacggca gaagcctctg cagattgaaa
     1681 gaaataaaaa tacgcgccag cggctccacc acccagcacg atcagcgctc ccaacgcaaa
     1741 caatgccagg ggcaggagat cccagggccg tggaatcgcg cgctcaggat agaacaccgt
     1801 cagcgacccg gctctcttga cgatcaacca gatcgtggcc gccacgacca ggatgaaaaa
//
